LOCUS       DASJTC010000001        92023 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.001, whole genome shotgun sequence.
ACCESSION   DASJTC010000001 DASJTC010000000
VERSION     DASJTC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 92023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.001"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(554..2167)
                     /locus_tag="U4B19_000001"
     CDS             complement(554..2167)
                     /locus_tag="U4B19_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2075775.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2308..3216)
                     /locus_tag="U4B19_000002"
     CDS             complement(2308..3216)
                     /locus_tag="U4B19_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2075776.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3399..4523
                     /locus_tag="U4B19_000003"
     CDS             3399..4523
                     /locus_tag="U4B19_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2075777.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4750..5667
                     /locus_tag="U4B19_000004"
     CDS             4750..5667
                     /locus_tag="U4B19_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2075778.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5660..6493
                     /locus_tag="U4B19_000005"
     CDS             5660..6493
                     /locus_tag="U4B19_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2075779.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6576..7694
                     /locus_tag="U4B19_000006"
     CDS             6576..7694
                     /locus_tag="U4B19_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2075780.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7688..9586
                     /locus_tag="U4B19_000007"
     CDS             7688..9586
                     /locus_tag="U4B19_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEN2075781.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9580..10803
                     /locus_tag="U4B19_000008"
     CDS             9580..10803
                     /locus_tag="U4B19_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2075782.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10889..12823)
                     /locus_tag="U4B19_000009"
     CDS             complement(10889..12823)
                     /locus_tag="U4B19_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2075783.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13049..13705
                     /locus_tag="U4B19_000010"
     CDS             13049..13705
                     /locus_tag="U4B19_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN2075784.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13779..14132)
                     /locus_tag="U4B19_000011"
     CDS             complement(13779..14132)
                     /locus_tag="U4B19_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN2075785.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14324..15253
                     /locus_tag="U4B19_000012"
     CDS             14324..15253
                     /locus_tag="U4B19_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEN2075786.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15264..16100
                     /locus_tag="U4B19_000013"
     CDS             15264..16100
                     /locus_tag="U4B19_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEN2075787.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16348..17124
                     /locus_tag="U4B19_000014"
     CDS             16348..17124
                     /locus_tag="U4B19_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2075788.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17300..18043
                     /locus_tag="U4B19_000015"
     CDS             17300..18043
                     /locus_tag="U4B19_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2075789.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18036..19616
                     /locus_tag="U4B19_000016"
     CDS             18036..19616
                     /locus_tag="U4B19_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEN2075790.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19867..20346)
                     /locus_tag="U4B19_000017"
     CDS             complement(19867..20346)
                     /locus_tag="U4B19_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN2075791.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20362..21114)
                     /locus_tag="U4B19_000018"
     CDS             complement(20362..21114)
                     /locus_tag="U4B19_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEN2075792.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21231..21301
                     /locus_tag="U4B19_000019"
     tRNA            21231..21301
                     /locus_tag="U4B19_000019"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21263..21265,aa:Gly,seq:ccc)
     gene            21662..22387
                     /locus_tag="U4B19_000020"
     CDS             21662..22387
                     /locus_tag="U4B19_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEN2075793.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22642..22908
                     /locus_tag="U4B19_000021"
     CDS             22642..22908
                     /locus_tag="U4B19_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEN2075794.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22905..23336
                     /gene="ruvX"
                     /locus_tag="U4B19_000022"
     CDS             22905..23336
                     /gene="ruvX"
                     /locus_tag="U4B19_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEN2075795.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23361..23666
                     /locus_tag="U4B19_000023"
     CDS             23361..23666
                     /locus_tag="U4B19_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEN2075796.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23747..24892)
                     /locus_tag="U4B19_000024"
     CDS             complement(23747..24892)
                     /locus_tag="U4B19_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2075797.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24953..25600)
                     /locus_tag="U4B19_000025"
     CDS             complement(24953..25600)
                     /locus_tag="U4B19_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2075798.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25794..26087
                     /locus_tag="U4B19_000026"
     CDS             25794..26087
                     /locus_tag="U4B19_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075799.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26131..26442
                     /locus_tag="U4B19_000027"
     CDS             26131..26442
                     /locus_tag="U4B19_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075800.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26439..27063
                     /locus_tag="U4B19_000028"
                     /pseudo
     CDS             26439..27063
                     /locus_tag="U4B19_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27204..27362
                     /locus_tag="U4B19_000029"
     CDS             27204..27362
                     /locus_tag="U4B19_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075801.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27363..27725
                     /locus_tag="U4B19_000030"
     CDS             27363..27725
                     /locus_tag="U4B19_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075802.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27762..28610
                     /locus_tag="U4B19_000031"
     CDS             27762..28610
                     /locus_tag="U4B19_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEN2075803.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28600..30075
                     /locus_tag="U4B19_000032"
     CDS             28600..30075
                     /locus_tag="U4B19_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEN2075804.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30341..30748
                     /locus_tag="U4B19_000033"
     CDS             30341..30748
                     /locus_tag="U4B19_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEN2075805.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30751..30966
                     /locus_tag="U4B19_000034"
     CDS             30751..30966
                     /locus_tag="U4B19_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075806.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30970..31350
                     /locus_tag="U4B19_000035"
     CDS             30970..31350
                     /locus_tag="U4B19_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2075807.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31484..31639
                     /locus_tag="U4B19_000036"
     CDS             31484..31639
                     /locus_tag="U4B19_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEN2075808.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31708..32181
                     /locus_tag="U4B19_000037"
     CDS             31708..32181
                     /locus_tag="U4B19_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEN2075809.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32178..33872
                     /locus_tag="U4B19_000038"
     CDS             32178..33872
                     /locus_tag="U4B19_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2075810.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33838..34023
                     /locus_tag="U4B19_000039"
     CDS             33838..34023
                     /locus_tag="U4B19_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075811.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34027..35202
                     /locus_tag="U4B19_000040"
     CDS             34027..35202
                     /locus_tag="U4B19_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2075812.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35195..36718
                     /locus_tag="U4B19_000041"
     CDS             35195..36718
                     /locus_tag="U4B19_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2075813.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36774..37058
                     /locus_tag="U4B19_000042"
     CDS             36774..37058
                     /locus_tag="U4B19_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN2075814.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37045..37380
                     /locus_tag="U4B19_000043"
     CDS             37045..37380
                     /locus_tag="U4B19_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2075815.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37675..38301
                     /locus_tag="U4B19_000044"
     CDS             37675..38301
                     /locus_tag="U4B19_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075816.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39043..39879
                     /locus_tag="U4B19_000045"
     CDS             39043..39879
                     /locus_tag="U4B19_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEN2075817.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLINLYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            39943..40374
                     /locus_tag="U4B19_000046"
     CDS             39943..40374
                     /locus_tag="U4B19_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2075818.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(40436..41758)
                     /locus_tag="U4B19_000047"
     CDS             complement(40436..41758)
                     /locus_tag="U4B19_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEN2075819.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41912..43552)
                     /locus_tag="U4B19_000048"
     CDS             complement(41912..43552)
                     /locus_tag="U4B19_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEN2075820.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43885..44355
                     /locus_tag="U4B19_000049"
     CDS             43885..44355
                     /locus_tag="U4B19_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEN2075821.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            44577..45680
                     /locus_tag="U4B19_000050"
     CDS             44577..45680
                     /locus_tag="U4B19_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN2075822.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            45964..46644
                     /locus_tag="U4B19_000051"
     CDS             45964..46644
                     /locus_tag="U4B19_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2075823.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            46734..47768
                     /locus_tag="U4B19_000052"
     CDS             46734..47768
                     /locus_tag="U4B19_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN2075824.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            47782..48117
                     /locus_tag="U4B19_000053"
     CDS             47782..48117
                     /locus_tag="U4B19_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075825.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48101..50581
                     /locus_tag="U4B19_000054"
     CDS             48101..50581
                     /locus_tag="U4B19_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075826.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            50637..52196
                     /locus_tag="U4B19_000055"
     CDS             50637..52196
                     /locus_tag="U4B19_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2075827.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52193..53557
                     /locus_tag="U4B19_000056"
     CDS             52193..53557
                     /locus_tag="U4B19_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2075828.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            53728..54798
                     /locus_tag="U4B19_000057"
     CDS             53728..54798
                     /locus_tag="U4B19_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2075829.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            54813..55481
                     /locus_tag="U4B19_000058"
     CDS             54813..55481
                     /locus_tag="U4B19_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2075830.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55501..56079
                     /locus_tag="U4B19_000059"
     CDS             55501..56079
                     /locus_tag="U4B19_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2075831.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56480..57391
                     /locus_tag="U4B19_000060"
     CDS             56480..57391
                     /locus_tag="U4B19_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2075832.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(57769..58686)
                     /locus_tag="U4B19_000061"
     CDS             complement(57769..58686)
                     /locus_tag="U4B19_000061"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEN2075833.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            58888..59325
                     /gene="zapA"
                     /locus_tag="U4B19_000062"
     CDS             58888..59325
                     /gene="zapA"
                     /locus_tag="U4B19_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEN2075834.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59383..59931
                     /locus_tag="U4B19_000063"
     CDS             59383..59931
                     /locus_tag="U4B19_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEN2075835.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            60046..62406
                     /locus_tag="U4B19_000064"
     CDS             60046..62406
                     /locus_tag="U4B19_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEN2075836.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62493..62807
                     /gene="trxA"
                     /locus_tag="U4B19_000065"
     CDS             62493..62807
                     /gene="trxA"
                     /locus_tag="U4B19_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEN2075837.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            62989..64767
                     /gene="uvrC"
                     /locus_tag="U4B19_000066"
     CDS             62989..64767
                     /gene="uvrC"
                     /locus_tag="U4B19_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEN2075838.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            64912..65811
                     /locus_tag="U4B19_000067"
     CDS             64912..65811
                     /locus_tag="U4B19_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2075839.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            65804..67066
                     /locus_tag="U4B19_000068"
     CDS             65804..67066
                     /locus_tag="U4B19_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2075840.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            67227..69125
                     /locus_tag="U4B19_000069"
     CDS             67227..69125
                     /locus_tag="U4B19_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEN2075841.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(69455..70174)
                     /locus_tag="U4B19_000070"
     CDS             complement(69455..70174)
                     /locus_tag="U4B19_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEN2075842.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            70511..70843
                     /locus_tag="U4B19_000071"
     CDS             70511..70843
                     /locus_tag="U4B19_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075843.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            70830..72833
                     /locus_tag="U4B19_000072"
     CDS             70830..72833
                     /locus_tag="U4B19_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEN2075844.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            72852..73322
                     /locus_tag="U4B19_000073"
     CDS             72852..73322
                     /locus_tag="U4B19_000073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEN2075845.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73343..73927
                     /locus_tag="U4B19_000074"
     CDS             73343..73927
                     /locus_tag="U4B19_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEN2075846.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            73942..74928
                     /locus_tag="U4B19_000075"
     CDS             73942..74928
                     /locus_tag="U4B19_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEN2075847.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            74918..75229
                     /locus_tag="U4B19_000076"
     CDS             74918..75229
                     /locus_tag="U4B19_000076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEN2075848.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            75251..77032
                     /locus_tag="U4B19_000077"
     CDS             75251..77032
                     /locus_tag="U4B19_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEN2075849.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77025..78401
                     /locus_tag="U4B19_000078"
     CDS             77025..78401
                     /locus_tag="U4B19_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEN2075850.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78408..79043
                     /locus_tag="U4B19_000079"
     CDS             78408..79043
                     /locus_tag="U4B19_000079"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEN2075851.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            79073..80428
                     /locus_tag="U4B19_000080"
     CDS             79073..80428
                     /locus_tag="U4B19_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEN2075852.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80620..82014)
                     /locus_tag="U4B19_000081"
     CDS             complement(80620..82014)
                     /locus_tag="U4B19_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN2075853.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            82106..83077
                     /locus_tag="U4B19_000082"
     CDS             82106..83077
                     /locus_tag="U4B19_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEN2075854.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            83074..83856
                     /locus_tag="U4B19_000083"
     CDS             83074..83856
                     /locus_tag="U4B19_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEN2075855.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84025..84396)
                     /locus_tag="U4B19_000084"
     CDS             complement(84025..84396)
                     /locus_tag="U4B19_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2075856.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84530..84973
                     /locus_tag="U4B19_000085"
     CDS             84530..84973
                     /locus_tag="U4B19_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEN2075857.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85016..85825)
                     /gene="yidA"
                     /locus_tag="U4B19_000086"
     CDS             complement(85016..85825)
                     /gene="yidA"
                     /locus_tag="U4B19_000086"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEN2075858.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(85844..87211)
                     /locus_tag="U4B19_000087"
     CDS             complement(85844..87211)
                     /locus_tag="U4B19_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2075859.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(87195..88025)
                     /locus_tag="U4B19_000088"
     CDS             complement(87195..88025)
                     /locus_tag="U4B19_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEN2075860.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            88188..88625
                     /locus_tag="U4B19_000089"
     CDS             88188..88625
                     /locus_tag="U4B19_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEN2075861.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88701..89321
                     /locus_tag="U4B19_000090"
     CDS             88701..89321
                     /locus_tag="U4B19_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEN2075862.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            89510..90016
                     /locus_tag="U4B19_000091"
     CDS             89510..90016
                     /locus_tag="U4B19_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEN2075863.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            90033..90932
                     /locus_tag="U4B19_000092"
     CDS             90033..90932
                     /locus_tag="U4B19_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEN2075864.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            91019..91903
                     /locus_tag="U4B19_000093"
     CDS             91019..91903
                     /locus_tag="U4B19_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN2075865.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
BASE COUNT        30402 a        15609 c        18941 g        27071 t
ORIGIN      
        1 tgttaaataa ataggaacaa aaaaattaaa ttatttaata cacacattag ggatcattca
       61 ttgaaggatt agaagacaac gaggatggcg aaaaatatga ttacaataga aaagtcgttc
      121 taaaccaatc agttcctagt atcacacaac gcaagccagt cgatttggtg agattagagg
      181 agtgaaagca aactttatta tataagaaaa aatattcgaa agggatgcta gtttaggtac
      241 tagtgctgat agattaatat gatgcctcgt aatattaggc gtagttactc tagagacatc
      301 actatggact atgatttgtc tcagagtcac aacatgtttt tttatgaaaa gaaacagcta
      361 aaataattaa attgcgcaat gtttaaactt tcttaaacta taacctaata aatgtgaaga
      421 aataatgcac aatttcttca catttatctt ctattctata tacataccaa ccaacaaccc
      481 tatatacttt ttcattttta ctcctcaagt aaaaataact cctagaccgc ggctacccac
      541 cgcggtcgtt tttttattct tcaatcaaaa tcgctttgat acccaaacct gccaagattc
      601 gggcagaagt ataagtattc aatcctcttc tgaaagtaat gtaaactttt ttatcttttg
      661 ggatttcatt gattcgatct cgtaactcat tcattggaat attttttgtt gccgagatac
      721 tgccagaagg tgctttatta ggttcacgta tatctaaaaa gaacgcttct ttcaaatcgt
      781 tttcgggtat atcacttgct ttgatcgttt ctacgatatt cgtcatttga ttgattgcga
      841 cataaccagc tatattcaac gggtcacggg tagtagaata cggtggagag taaggcaact
      901 ccaaatcagg caaatcaaag actgtcaagt ttccggtgat tgctacagaa agttctccca
      961 tccgtttatc gacacctttt tctccaaatg cttgtccacc taaaatacga ccgctatctg
     1021 catcaaaaat cagctttaga tttaatcttg tagcttctgg atagaaatag gcatggtcaa
     1081 agggagtgat aaaaacagtt ttataatttg ttatgcctga tgcttttaat tcatgttctg
     1141 tcattcccgc ataacttgct gtatgatcaa agatttttgc gacacctgca ccaatatatc
     1201 cgcgatagcg cataggcgta ccatttaaaa tatctgccag catatgtcct tgacgattgg
     1261 ccgcactgga aagcatactt tggatcggct gaccagtcac tacactagtc gtttcgataa
     1321 tatcgccaat cgcatagatg tccggcagat tggtttgtaa ctggtcatca acaatgatct
     1381 gacctgtatc tgataattgt atgccggttg ctttcaccac ttcattcttc ggagaaacac
     1441 caacagcaaa aattagcata tcagcagaaa ggacggagcc atctgataat actatttctc
     1501 gacctttatc acggatctca gtaactcttg tttctaaatg aacggctaat ccttctttca
     1561 gcaatttgtc ataaactaaa ttagcaattt ctggatcata agggaaagcg acttgtggca
     1621 attgatcgat taaagtcacg tctaagccgc gatgcttgaa attctcagct aattccagac
     1681 ccataacacc tgcaccaaga atgacgacat gttgtggatt tttttcttca aggaacgatt
     1741 tgattcgatc cgcatccgtc acactacgga ggacaaagcc gttttctgct tcaaaaactc
     1801 cgggaatatc cggataatct ggtctagcac cagaagagat gatcaactta tcgtaatctg
     1861 tttttatttc ttcatttgtg gataggtctt taactgttaa tcgcttagta gaaggatcga
     1921 ttgctgttac ttcgtgttta gtaaagacat cgatgttgtt ttttgttttc aatatttctg
     1981 gtgtacgttc gatcagcgag tcacggtcag tgatcacacc tcctaaataa taaggcaaag
     2041 cacaacttgc ataagaaata ttttctccgc gttcatagat aatgatttcg tgcgcttcat
     2101 tcaatcgacg gaaacgagtg gcaaatgaag gaccgccagc tacaccgcca atgacaacaa
     2161 ctttcataaa aacatctcct tttctacgtt gatcgtacta tgttatggta aaaaacagca
     2221 aataattgtg ccggaaaatt attgtttatt ttgaatagac agaaaatata gagaaataat
     2281 ttgtggataa aacctaaaaa tttttttcta ttttaaggat agataatact ttgaagggga
     2341 taaccctgta tattttttaa aagctgaaga aaaatgatgg atcgaatgaa atcctaatat
     2401 atctgcaatt tctgtatagg ttaattcatt ttctagcacg agacgttttg ctttttccat
     2461 ttttagttct ttaaaatagc taataacact ttttccattc acttttctaa aatgtttttt
     2521 taagctggct ggactgataa aaaaaatttg ggctaattgt tcaagcaaaa aattttctga
     2581 aagatgttct tctaaatatc gtatgattgg atcaactaat tcatctgaat aaagaatatg
     2641 ctttttttta ggaatgactt tctcatttga aggtattgtc aataaagcaa gttcctgttg
     2701 ataatgcctt ggtaaggaag acaattgaat tttttgcaga caataagtaa ctaataagga
     2761 aagcaaatga atgctcatct gttggcatag tatgaaaaaa gaattgattt cctgatcata
     2821 ggaaatagct ggcttactat gttttagata atcaattttg aactgttcat ttttcagaaa
     2881 agtaatgata gatgttattt cttgatctat agaaaaagat tgtcccgtaa tatgtgctaa
     2941 taattgctca tcaaaataaa actggataat gataaaagtt tgtgtttcct tttgagtaga
     3001 aaagatttgg acttcttgct gtttatcaat gaataaaagg gaaggattat ctagttctat
     3061 agatttatgt gcaaattcag catataaatt agcgctttca tttattagta gtcgaaaata
     3121 gggagcagca gataaatgaa gatgggtatc tatggaatag gttagtgttt ttttgacaag
     3181 attggaggat tggttgtttg ttaacaaaag aaccactttc ctttctcttt ttatataaaa
     3241 agctttctat ttataattaa tgactattat aacaaaaatg aaaagccaaa acgacaagta
     3301 aaatattttt ttatataaat atttgtgtta ctaattccgt taatatacat aagtgaaagc
     3361 gttttatata agaacttgaa aagaaagaag ggatagacat gaaaaaaaga tacaaattgg
     3421 tgatagcttt gctaaacatt accctattat tcagcttaag tgcatgtgga aaaactaacg
     3481 aagaagtaac agcaaacatc gcagatccaa tgataaaaaa agccactaca ttagaagtat
     3541 atgtcgcaaa cggcaaactg acaaactatg ttgaaaatgc tgcaaaaatc tataaccgca
     3601 aaactggagc aaatattgac ttaaaaattg tgaatgtagc ttcgggaaca gcaacagtac
     3661 aaatgatcac cccaaaattg gtaacacacg aagatatgcc ggatattatc tccatggcag
     3721 acacttcagc agcaggtgtg ctggaaaaat ttcaagatgc tttttatagt gctgatgaat
     3781 atggatttta tgacaaatat ggcgatcagt tttatgatca aaaattgaat attttgcgtc
     3841 aacaaacgag taatgaaacg attattcctt gggctagtga ttttacccca gcactttgtt
     3901 attatcaacc aaaaaaattt gctgaaatag gtgtagattt tgaagaaatc agttcttggg
     3961 aagaatatat ccaggtggca aaagcattaa aagaaaaaac tggtagtttt ggaattgcct
     4021 tgccagaatc tggggaccaa gaattgttta tcaacatgct agcccagcag aatcaatctt
     4081 tgcttgatga agaaggaaat atccgtctga atactaaaga agcgaaacat gctgcaaagt
     4141 taatcaaaga aatgatcgac tcagatattg tacatttcta cggtgcacaa gatgctgaga
     4201 aagcatttca agaatctgca atgtttgttg ctggcggctg gtatgctacg aatatgtctt
     4261 tgaattttcc tgatgcatct gacaaatggc gcatggctcc actgattcct ttttcaaaag
     4321 aaaatccagg aaaaggtgca gtatcaggag gatcaagttt ttatgtccct aaagatgcaa
     4381 aaaatccaca agtggcacaa caatttctta catttatgct gacagatgaa gaatgcttag
     4441 ccaacgcact ggagctgggc gtggctacat ctaactcatt ggcctatcaa acagaagcag
     4501 ctgagaaaaa gtttgactac taaggaggac aaaaatacta tgaactattg aataaaatca
     4561 atgaaaatac atcaaatact gttttaccag ccacttattc agatgctacg tcttatttga
     4621 ctaccgctgc atataactat tggcaaacag gtgattttga aaattcatat ctaaaagaag
     4681 cagaaaattt tgctcataag tacggcgtaa aattaactgg tgattaaaaa atattaggag
     4741 gacttaacga tgatgcaatc ttcgttacca agcaagacaa taaagaacag caagaaaaaa
     4801 gcaacagata cgtctcataa aaaaagtgct tatggattgg tcacaccttt tgttattttc
     4861 tttattgtga cgattttgat tcccatcatt atctcgtttg cttttagttt tatgaaaatt
     4921 ggctactcat ttgaatttgt tggatttgac aattatctag ctatcttccg agatccattg
     4981 tttattaaat cttacacgaa tgttttgatt atcatggcag gatcaattcc aataacgatg
     5041 ctattagcta tttactttgc tgttttattg aattcagcac agttaaaagg aaaaggattt
     5101 tttagaactg tctattatat tccaaccatt acatcaacgg tggctgtagc ttccgtattc
     5161 atgacttttt tcaatccgac tgggttattc aatggatttt tgaatgcgct aggaattaac
     5221 ggtgtacagt ggttaacaaa tcctttttgg atacgaatat caatgatcat cacgatgatt
     5281 tggcttagtg taggatataa cactatttta tttttagctg gtcttcaagg gatttctaaa
     5341 gagatatacg aaagtgctga aatagatgga gcatcgaaat ttcggcaatt ctttttatta
     5401 acgatcccca tgctaaagcc aatcatttta atggccacag ttttagccac tatcaatggc
     5461 ttgggtaatt tcaatatccc aaatatattc tttgggactt ccaatggacc agaaaattca
     5521 gctttggtcg taggagtcaa tttatataaa acaagttttg aaatggttga ttttggaaaa
     5581 gcctctgcta ttgcctggac gatggtgttt gtatctgtta tattatcggc tattcaattt
     5641 aaattcggag gtgataaaaa tgactaaaca aaagatacaa agcagaattt ttacgtacgt
     5701 cattattgtg tttggtgcct taatattttt gatgccatat ttatatatga ttgtagcttc
     5761 aacgcaaagt aatcaagaaa ttttaagtgg cggaatcaat ttcaagtttg gggatatgct
     5821 attaaaaaac tggcaagcca tcatggcgag atataattat cccttgatta tttggaattc
     5881 gattgttatt gctgtaatca gtacagtttt agctacagcc agttctacaa tggcgggtta
     5941 tgcattagca aagtatcgtt ttatggggaa taaactgttg ttcaatacag ttatgctatc
     6001 taggatggtg ccttttttta ccacattgat tcctttattt tacattatga gtcgtttagg
     6061 attatcgaat acttatgcag gtgtgatcat cccttcgatt gccagtacaa cttcagtgtt
     6121 tatgatgcga caatatagca gtcaatttcc tacagcactg atggaggctg ccagaatcga
     6181 tggtgccaat gagtgggtga tttttacgaa aatcgctgta ccgattttat cacctacgat
     6241 tattactagt ggtttactta tctttatggg aagttggaat caatatctat ttccattagt
     6301 catgctttca gataaagata aatatacggt tcccttaatt atcaaaaata tgacggtttc
     6361 tagtacaggt gagccaatta attatggcgc aattatgcta attttagcaa cttcagtgat
     6421 cccaatggtc ttgatttatg tgtgggcgca gtctaaattt aaggagaatg acattggagc
     6481 tgcaaataag taaaaaaaat aacgaatgtt aaccatctag ttcgttctag gagcaactcc
     6541 acattcataa aaattaaaag aatggcggga tatccatgaa tgaacagacg aaggaatggt
     6601 caagaaaaat tttgaatgta gcttctgaaa agatagatgc ggaattagac agaatgggga
     6661 ataaaattcc atatatttct tacaacggaa aatatacaga cgtaataaag gacagaggaa
     6721 tcgactggtg gaccaatggt ttttggagtg gtattttatg gcagctcttt cacaagacaa
     6781 aacgagaaaa gttcaaagag gcagcaatac aacaagaaca attattagaa tctgccctgt
     6841 accatttttc agacgttcat catgatgtag gttttttatt cttaccatcg gctattgcac
     6901 attatcaaca gtctcaaaac aaacatagtc gacaactagg gttacatgca gctacggtac
     6961 ttgctggacg ctataatatt tcagggaact atttagtagc atggaatgac cataaacctg
     7021 gttggatgat tattgatagt atgatgaatt taccattact gtattgggct tcgagggaaa
     7081 ctggtgatcc gaggttctct aaaattgctc ggcagcatgc agatacaaca gctaagtatc
     7141 tagtgaggaa agatggatca accgggcata tcgcagaatt tgatgcagaa tcagggaaat
     7201 atattcggtt acttggcggg caaggataca atgagaattc ttgttggagc agaggaaatg
     7261 catgggcttt atatggattc gcgttaagtt atcgttacac aaaaaatccg tactatctag
     7321 ctatagcaga aaagatagca gtaaatttta ttcagaaaat cgaaaaaact ggttatattc
     7381 ctctcattga ttttgctgta aaagattcga atattgatac cagtgctgga gtatgcgctg
     7441 catgcggtct tctggaatta gcaggatatg tttcctctgt acgatcaaaa atgtatcatg
     7501 aagctgctat caatatactc caaaagacag aaaagcacta tgcaaattgg aatatggatg
     7561 aagatgggat cattggaggc ggagcagaag cataccatag agaactgact tataatgtgc
     7621 cactgatcta ttctgattat ttttttattg aaggcttact tcgattagaa cagtcggaat
     7681 tagctatttg gtagtaaaaa gaggagaaaa tacaatgaaa catccagtac cactattaag
     7741 gaccagacag gattactttg acttatttaa aaagataaat caaccactcc agccgtttta
     7801 tcgagcaagt ggcgcacaaa ttgattatat acatcaaggg gtaggatatg gaaataaaat
     7861 tgcaggaatg gaaggattct ctcgggtttt atggggagcg gggcccgcaa ttgaccaatt
     7921 agatgaacga tggttgaaat taattctatc aggaataaaa gctggaacag atccttccca
     7981 tcccgattat tggggagaaa tacatgaaag agaccagcgt atggtagaaa tgcccgcaat
     8041 gttattagcg ttatatcatc atgaacaatt attatggaaa aaattagata caggagaaaa
     8101 aagccagatt attcattggt tctcgcaaat ttttgaatat gaatgtgcag atggtaattg
     8161 gcaatttttt aagataattg tcggaacaat tatccaaaaa tttggttatc cagttaaaga
     8221 atcagcactg aaagaagcat ttgataatat agaaaattgt tatcttcaaa atggctggta
     8281 tcgagacagt tcaagaggtc gccaagatta ttataatcct tttgcatttc attattatgg
     8341 tttgatttat agcgtactag aaccagaaaa gcccattagc cagatatata agaaccgagc
     8401 tattgatttt tctagagact atattcattt ttttgcggaa gatggtgcaa atgtgccttt
     8461 tggtcgaagc atgatttatc gttttgcagt ttcaagcttt tggtcagcta tgttgtgggc
     8521 cgatcttttg ccttttaaat taggtgaaat caaggggtta gtcaatcgga atataagatg
     8581 gtggatgcaa aaagaaatat ttgatgaaaa aggtatcctg aatattggtt atacctaccc
     8641 tcaattgaca ttgactgaac cctataatag tacgttatct ccattatggc tgaataagat
     8701 ttttttattg ttggcgctac ctgaagacca tgagtattgg acggctaaag aaatgcctat
     8761 gcctattcgt gaaggaacta aacttctttc agaagccaat ttgctcatac aacatgataa
     8821 tggacataca ttttttttga atgcgggaca attagggcca aatttccata cattgaccaa
     8881 tgaaaaatat ttaaaatttg cttattctag tcaatttggt ttttcgatac cgcgagcgaa
     8941 tcaattgaaa gcagaaatgg caatggattc aatgctaggg attcagcgag cggacacata
     9001 tatcacgacc agttttaaga atgagagcat caaaacctat ggacagttca tcgttcgaaa
     9061 taatgtacga gacattgtat gtaagaaaga ggtacttgcc tctacatggg aacctacttt
     9121 acaggcaaaa atcagaactt ggcttattcc ttttgaaggc tggcagattc gcgtacacaa
     9181 agtagaatta gatacagaat atgttctata tgagacaggg tttgctattg aatgttctcc
     9241 tgagaaagaa ggaataatca tagaagaaga caaggaaaac tatagggtga gtgaagctgg
     9301 cttttcaggt attatctgtt taagtgacga tactattaaa cgaaaatcca cagctattat
     9361 gggtttgccc aatacaaact tgatgacatg ggaaaacact tttattcctg gattagaggg
     9421 tacatttgga aaaggaaccc attggctcgg aacaggagtg gttgctcatc aagatcggga
     9481 ttatagttta gaagtatgga acaatcgtcc caaaattgat tttgatggga caaccgggct
     9541 aactatccaa aataaaaaca ataaaaaaag actaaaatta tgctaaaggc tggtggggaa
     9601 atgcttgata aagtgatgtt tgaagagata atacatccag aaaaatggat aaatgaatgt
     9661 tataaagaat taatggaaaa agaatggaat aatgagatcg agcgttgtcg cagacacacg
     9721 gagaggttaa tcaaacgtat cagtaacagt cagtatcaat tcccaagcga gtgtcctata
     9781 aaaggacgtt atccattaag agacaatatt ggttggacag aaggattttg gacaggaatt
     9841 aattggttgt tatttgagaa gacgggtgat cctttatttc aacaaacagc tgaaaaagca
     9901 gatatagatt ttatccacag gattgactgt caaattatca ctaatcatca cgatcttggt
     9961 tttttgtatt ctttatcaac cgtagcagat tataaaataa cgggaaatcg aaaaatgaag
    10021 gaagcttcaa ttgatgctgc agaaacatta cttcagcgtt tttgggagaa ggcaggagtt
    10081 atacaagctt ggggagatcc tagtgatcct tcacaacaag gtagaatgat catggattgt
    10141 tgcatgaatc ttccattatt gtattgggcg agtgaagaaa ctggaaatca aaaatataaa
    10201 aaagctgctc aggctcatat tcaaaatgca gcagattatt tagtaagaga agatggttca
    10261 acttatcata cattttatat ggatgtcaaa gatggaaaac caagatatgg aagtacgcat
    10321 caaggttata gtgataattc ctgttgggct agaggccaag catgggggat ttacggtttt
    10381 gcattaagtt atcgttattt gaaagaacaa aaatattttg atttaagcaa aatattagcc
    10441 aattatttta tgaatcggtt gccagaagat accatttgtt attgggattt gatctttgat
    10501 gattctagca gagaagaaaa agatacatct agtatggtga tcatggtttg tggattatta
    10561 gaattaatag aatcaggtct attagacaaa acagaaaaga acttatacca gtctgctgtt
    10621 tggaaaatta tgcgacaatt gaaagaaaga tatgctacaa cggaagaaga agatggcttt
    10681 ttaacacagt ctgtttataa taagcccaaa aataatgggg taaatacacc ttgtatttgg
    10741 ggtgattact attatttaga agctttagtg agaatgacta gtaattggtc ttcttattgg
    10801 tgaaaaaggt aaatatggaa tttttgtttg ataaaataaa aaaagaaaaa ccaaaacagc
    10861 atgaatggtt tctgttttgg ttttttcgtt aattttcata ttcttctaat tccaagctta
    10921 gttcttccca ttccgtcaat tttgtttctt gatcttgtct agcagcttct aaatcttggt
    10981 tcagctcatt gagttttacg tgatcttcca ggttttctgg cgcgaccatt tcttgttcca
    11041 actgatcgat ccattcatcg atccgcgcca tttcttcttc aacctgagta atttttcgtt
    11101 gaaggctacg gataagcttt tgctgttctt tgctttggta ataatcagat ttgttcgtgg
    11161 gagcaggagc agattctggc tcatcttgtg ccaacaattc ggcgatttct tcttcttcct
    11221 gctttttctc taaataataa tcatagtctc ctagatagac tttgctgcca ttttcagaga
    11281 gttcgacgac ttttgtcgcg atccgattga tgaaatatcg gtcatgggaa acaaaaagca
    11341 gtgtgccgtc ataatcgatc aatgcatttt ctaatacttc tttgttgtca atatctaaat
    11401 ggtttgttgg ttcatccaaa atcaaaaaat tttctttgtt catcgctaac ttggctaaag
    11461 caacccgtgc tttttcaccg ccacttagca gagggattgg tttttctaca tcttcgccag
    11521 aaaataagaa agcacctaaa gcacttcgga tctctttttc tggagtggta ggatgctcat
    11581 cccacaattc tgctaggatc gtcttgtttg tatgcagatc agcttgacct tgatcgtaat
    11641 agccgatggt gacatttgtc cctagcgttt cttttccacg gataaaaggg atctggtgaa
    11701 tgattgattt cagcaacgtc gatttcccga taccattcgg acctactaga gcaatcgctt
    11761 cttgacgacg gatatctaga ttgattggtt cagataaggt gacattatca tatccgatag
    11821 cggcatcttc gatttgcaag actacatttc cagaaggctt ttctatactg aatagaaagt
    11881 ttgcttgttt ttcatcccct tgaggacgat ccagccggtc cattttttct aatgtctttc
    11941 tgcggctttg cgctcgtttt gtggtagagg cgcgaacaag gttccgagca acgaagtctt
    12001 ctaatttatg gatttctgtt tgctgctttt cataagcctt ccattcactt gctaactgtt
    12061 ctgctttcaa atctagatat ttcgaatagt tacctttgta atggcgcatt tttcttctgc
    12121 ttaattcata gacttcattg actactttat ctaaaaaata acggtcatgg gaaacaatca
    12181 ataatgcacc agtatagctt tgcagataat tctcaagcca ggaaagtgtc tcgatatcta
    12241 aatggttcgt tggttcatct agaatcaaaa tgtcagggtg ttgcaaaagc atgcgagcta
    12301 aagctaaacg tgtgcgctgt ccgcctgata aagtatcgat tttctgatca taaaatgaag
    12361 aatcgaattt aaacccatgc aatacggaac gaatttcatt ttcataacca tagccgtttc
    12421 gatctgaaaa atcgtgttgc agccggtcgt attcttttaa ataagaacta taagcaggtg
    12481 tgtctggcgt accttcgcta atgaaaatct ctagctcgcg catccgctct tccatttgtc
    12541 taacttgttc aaaagctttc agcatctcgt tccaaactgt ttcattggaa tcaagtcctg
    12601 tgtcctgagc gaggtatccg agagtcgctg ttttattttt agcgatcttt ccttcatcag
    12661 gtgcttcaat tccagcaatg atctttaata aagtcgattt tccagcgcca tttctgccaa
    12721 ctaaagcgac acgagcgcct gtactgattt ctaaatgtat gttttcaaat aagacttctg
    12781 caccaaaatg gcgagcaatc tgattggctt gtaataatat catggaatcc tcttttctac
    12841 tttgtgaatt ctcctcaaat aaaaaactat ttttcaatta gtagtctacc ataaattcca
    12901 ttatactgcg agatttgcag aaatgataaa taaatgaata aagaataaaa tttcacaata
    12961 tctgagcaat ggaattgatt ttttcctata aatactgcta tgatggagaa atcaaagatg
    13021 taattagaca atacgtggag gtcatttcgt gaaagatcat attattccaa aggcgacagc
    13081 taaaagactg cctttgtatt atcgctattt aaggatttta aataatgctg gaaagaaaaa
    13141 agtgtcttca actgaattaa gtgaagccgt tcaagtagat agtgcaacga tccgtcgaga
    13201 tttttcttat tttggcgaat taggaaaacg tgggtatggt tatgatgtag aagatcttat
    13261 gcactttttt gcaaagacat taaacgacga tcaattgaca aatgttgcgc tgatcggtgt
    13321 agggaacttg ggaagtgctt tactaaaata caaattccat caaagtaata gcatacgtat
    13381 cagttgtgct tttgatgtga atgaggaaat cgttgggcgc atcgtagacg gtatccccgt
    13441 ttatccaatg gaagatatgt cagaacaaat caagatccaa caaatcgaag tagcaatttt
    13501 aacgataccc gctgaaagag cacaagaagt agtcaatcag ttaacggaag caggaatcaa
    13561 aggaatcttg aactttaccg ctgctcgatt gaatgctccg tcagatgtat tgatccaaaa
    13621 tgttgatttg acgaatgaat tgcaaacctt gatctatttc ttgcattctg acgcaaatgc
    13681 gcattccgaa aatacagcag aataaaaata aaaagcgatt gaacaaatga aggcaaaact
    13741 ttcacttgtt caatcgcttt tttctgtttg gacaacgatt attttgttcg gaagatttct
    13801 tcataagaaa gggaatcatc ttctgctgtc agttcatcta gtagataacg gattttttta
    13861 aagtcgatga accgtgcttg ccgaaagact tcttttcctt gatgaaaaat caggacaact
    13921 ggcgcagtca gcacttcaaa tttactagct acttctggtg tttcaaatgc atccaaatga
    13981 agtgccggaa tatcataatg tgtgagaagt tcctctagtc gtggacgaac agcgtgacaa
    14041 acactgcaat taggcatcga aagataaatg acggcaacgg atgactgttg cagtatgtct
    14101 tgaacttcag tcaagttagt ggtttgtttc atttttattc tcccttttat gatagaagtg
    14161 atatgtatga gaccttaatt taatgggaat aacagaaaaa gtcaattgaa gtgctttggt
    14221 aaaaaataag agttgtgtaa aaatttacag agcaacttgt tttttttgaa taaaagcgta
    14281 tatttgcaaa gcgaatgaac tagaaataaa aaggaggaaa ctcatgtttt cttttattcc
    14341 aaattcggtt ttgcagatga gtacgatttt cttatcgatc gttattgaag cattaccttt
    14401 cgtattatta ggctgtctta tctcaggcgc cttacaagta tttttgaccc cagaacgtgt
    14461 gaagagatgg cttcctgaaa atcgtatctt ctctattttt actggttcga tcttaggatt
    14521 tttctttcct tcatgtgaat gcggcatcgt accgatcgtt catcaatttg ttaaaaaagg
    14581 cgtaccagtt cacacagcgt ttgcttttat gctgactgct ccgatcatca atccaatcgt
    14641 gattttttcc acttttatcg catttggcaa ttcttggcag atggcaagct ggcgggtgat
    14701 aggcagcttt tttgttgccc ttgtgattgg tgtctggctt gcctattttc aaaaagacgc
    14761 tgtattgaaa gaaaaaatca cgtctaaagt agcagaaaat caccatcatc atgtccatga
    14821 agaagaacaa agtgagaaga atgaaccaaa aaatcagctg atccatcaaa ctgggcatgt
    14881 gttgacacat tcaatcgacg aattttttga tacaggtcgt tatttgatca ttggcggatt
    14941 aatcgcagct gcgatgcaga cttatttacc aactagaatt atgctaacgc tcggctcaac
    15001 aaagatttta gccattatca tcatgctgtt attggccttt acgatgtctc tttgctcaga
    15061 agcagatgcg tttataggat cttctttgtt aagtctgttc ggcacagcac ctgtagctgc
    15121 ctttttggtc tttggcccaa tggtcgatat caaaaatcta ctgatgatga aacgatattt
    15181 tacaggacgc tttattttat cgctgatcgg actgattgcc cttagcgtta ttggattcac
    15241 attatttatt taggagggaa aatatgctgc ggtttttgat tttatctggg tatttcgaat
    15301 tgatgatgta cttgcaagtt tctggcaaac tcgatcaata tatcaatgtc cattatcgct
    15361 acttagctat tctttcaatg attttatctg ctcttttggc tttagttcag ctgtatttgt
    15421 gggtcaaaaa cggaaaagca gaagaaaagg cacacgagca cgatcatgac catgggttgt
    15481 caaaacctta tcaacgtgga atcgcttatg tactgctagc tttgccagta atcgtgggga
    15541 cgttgtttcc aacagttagt ctcgacacta cgatcgtaga agccaaagga tttaatttcc
    15601 cagtaagtaa agaatcagta ggggatccgg agatgcagac acaatactta aagccggata
    15661 ccagtattta tttcaacaaa tcagactact tagaccaaat gaatgagttg aaagaaaagt
    15721 atggggataa gcaaactatc aagatcacag atgaaaacta tttagaagtt atggagctaa
    15781 tttataatta ccctagcgaa ttcattggca aaaaaatcag ctatgaagga tttgtttacc
    15841 aaacaccgaa cgatagcaat acagatatct tcttgttccg attcggcatt atccattgtg
    15901 tggcagattc aggagtcttt ggtttgttga cgcatatgcc ggaaggagta acggttaaaa
    15961 atgatgaatg gtataaaata gaaggaacca tccaatcaga ctattatcaa ccattcaagc
    16021 gtgaaatccc atctgtagtt gttacaaacg ctgagaaagt ctctgctccg aaaaatcagt
    16081 atgtgtatcg ttctttttaa aagaacaaaa taaataaaca tagctcagac agctgacacg
    16141 caaggacgtc ggttgtctgg ctttttttgc atgatgtatt catttgtttg atatttattt
    16201 tagaagcagc atcctgaaag tatttgatat cggtttcaaa ggtgttggaa aagattgtga
    16261 agagaattac aatttattcc taacatgtat gttgaaaagt tgtatgaaat gattttgata
    16321 gtaaaatatc gagtggaggt actttttatg gtaacgcgta tatttatttt ggatgatagt
    16381 ttggtttttg aaaaaatgct agtagaaatt ttggaagaat ccagaatttt attaagtcct
    16441 tggaaatttg agatcgttca gggattgaac gttatacaat ttgttgaatt cattaaaaat
    16501 aatgaaatct attcctcaga tatttttttc ctagacatcg atttgaacgc ctattatagc
    16561 ggtatcgaac taggcgaaat gcttagagaa aagaatgaaa agtgtaaaat tgtttatcta
    16621 acgtctcatc cgaataaagc aataactgtt attaatcgaa agattacgcc tagtggttat
    16681 ttgataaaag aattcactta tgaaaaaaat aaaaaaagca tccaaagtta cttattgaaa
    16741 gaaaaagaaa actatcctgt tcaacatgct acgaaaaaaa ggtggacaac tgtccgttat
    16801 ggtaatagcg atcactatat ccattacgat gaaatccttt atctaatgag catgccaggt
    16861 tataaaaata aaataggcct agtattgaaa gatgaagaga ttctcgtaaa cggcacaatt
    16921 aaaaaatata aaaaatcact cgaatatgag tatttatgta aagatttaaa agcttatatc
    16981 attaatactg aaaacattac atctattagc agagtaaacg aaatagtaac atttgcaaat
    17041 ggagaacaac tagagatggg aaagagaagt attgataaac taaagaattt tatgcaagaa
    17101 aagatagaaa atgaatcatt ttaaagaaaa caaagactca tatcttacct gttagaagag
    17161 tgttttcctg tagaagagaa caacccaagt agcagcctag tcgtttctat ccttttttgt
    17221 tatgcactct ttttgacatt tactccacca ataacgaagg gtacaataaa aagtaataac
    17281 aaacaaaagg agtacagaaa tgattgaatt catcaatgta agtaaaagtt acgggaaaaa
    17341 acaagcatta aaagacttgt ctctctcgat aagacaaggg gaaatttttg gatttttagg
    17401 acataatggt gccggaaaat caactacgat taaaagttta gttagtatta ttgaaccatc
    17461 atcgggaacg attttagtag atggaatgaa attgacggaa aatcgattga gtatcaaaca
    17521 aaaaatcggt tatgtgcctg attccccaga tattttttta caattgacgg ctggagaata
    17581 ttgggacttg atttcagctg cctatgaatt agacacacaa aaaaaagaaa agcgtttagc
    17641 agaattgaca gcgctttttg atatgtatag tcatcaaaat gaaacaatcg caagtttttc
    17701 acatggaatg cgtcaaaaaa cgattttgat cggcacgttg ctgccagatc cagatatttg
    17761 ggtattggat gaacctttgc aaggacttga tcctcaagca gcatttgatt tgaaagaaat
    17821 gatgaaggct catgccgcaa aaggaaaaac agtcattttt tctacacatg tattggatac
    17881 cgcacaacag ttatgcgata aactggctat tttgaagaaa ggtgaattga tttatcaggg
    17941 ggcagttagt gaattattga atggctcacc tgatgaaacg ctagaaaaga tttatttgaa
    18001 aatggctgga aggcaagcga caggagatgg accgtatgta taagcgacaa ttattagaat
    18061 taattcgagt taatctttta tatgttaatc cacagctcac gaataaagca agagagaaag
    18121 gaaaaaaggg aaagcgatta actatctatc ttttgtctca aaatcttttt tcagggctga
    18181 tttttttact gatttacgga tttgctatgt ttgctatgaa ctttgttaaa ttgccaggtt
    18241 tctttactta ttacgtggca ctttttggta tgttaggttt aactcaaagt atatcagtca
    18301 tttataatat cttttttgaa ggaaatgacc tgcaagtatt tttgcctctg ccttttggac
    18361 aagaacaggt tttttcagca aagattttgg tagttgcttt gaccatcatt ccttttgttt
    18421 ttccgcttct agtcatgttt attttaaccg gatggcgagc tgatctaggg attttgctta
    18481 ctatcattat ctcaatcctt ttgttttctc tatttttgat tattcttttt tgtctggcta
    18541 gtttgattgt ttttggtttg gctcgaacag cagtgtttaa aaaacataga aaaacagttt
    18601 ctagtatctt gcttggcagc tcgatgctga tcgcatttgc tggaatcatg tggatgaatc
    18661 atcaaacggg gactattgaa aatgagttaa cagatcggca tccgatttcg atattattgc
    18721 cattctatga tgctgccagt cagccacttt ctcaacaagg tttattgggt tggggaatac
    18781 tagctatcac ggctctggga tttttccttg ctgtaaaaca gctgatagta ccaaagctta
    18841 cagagcaact gactgattca aacagtgagt tcaaaataaa aagaacccat aaaaagaacc
    18901 aaaatgtgca tcagctttta tttagttaca atgctcaatt agtaaaagaa cctaatttga
    18961 ttatgcaagt tctttcttct tccttgttaa ctccaatcat ttttattctt gcttttgccc
    19021 taggaggcga aataaaatta actgatttag ataataagtt aatcggtgtt gttttcctgg
    19081 tagggatcgc attggctttc ttaacagtta atcagacttc ttttatcagc aatcttattt
    19141 ctttggatca agaaaatttt ctgtttatcc gttctttgcc aatctctatg aaccaatatt
    19201 taaaagaaaa gttcagattc ggatggatat tgcagagttt tttaactggt gggatcgcat
    19261 tattaagcgg actgtctttt cagctgcctt tgttttttat ccctagcttg ctatttggtt
    19321 ctttattcgg ctgctactta ttgtcactac gttatttcgc tagagattat cggttgctgc
    19381 tgctgaactg gaccaatatt aaccaattat ttaacagagg ttcaggaagt ttgggtttag
    19441 tcgcaactat gatgggttca ttgatcatca gtattattct tatcgtctta tacggaatgt
    19501 tggctttgtt ttatccattt tggttagtga atattcctgt gatcggttta gttctaatac
    19561 tttccgctct atggatcgtt tattatcaac gaacgttttg gaaaaaattt gaataaagaa
    19621 gaggggccgt gacaaaaatc atgtcgcagc ccctttttta cagatagagg gtgttcaaaa
    19681 gtcagtttct cagtattaag tcaattttat gtaaaaatgt gagaagctat ttttacaaaa
    19741 ctttttttaa tactcataat tacttcttat tgctcctgcg gttacttgtc gcaatgtttg
    19801 tgagatattt tgtcatcgct caaggctgaa cgtctctttc acaacctttt ctttgttaca
    19861 cttcgtttat ttaaaaagcc gtttttttcg tttcatcatg attgcgtcac gctttacttt
    19921 gaaattacgg atgttatggt taaagacttc tgataataca agacctaaag cgattgctcc
    19981 agcaatcata attgcttgga ccgcataatg agccgcttct cgataggatt cagtaacgag
    20041 atttcgaacc gcttgataag ctaaaccacc agggactaga ggaaccatcc cgggaatatt
    20101 gaaaatcgtt acgggcattt tcaaacgttt agagaaaaga tcgctgatga atgctactcc
    20161 taaagaacca agcaatgaac caaaagcagc agatccgcct atttgaacac agatccagta
    20221 gatcatccag ccaaaagcac cagaaagacc acaacaaatc aatgaacgcc taggtacatt
    20281 tgtaataacg gcaaatgccg cagtcgctaa aaaactaaaa gaaaactgaa taaatatcgt
    20341 ccccatactt gtgttccttt cctaataaaa aagctggaaa ataaaagcaa tcgcaaaacc
    20401 gatcatcgta gcagtcatca tcgcttctgt tccgcgagaa actcctgaga cataatgtcc
    20461 agctagcaaa tcacggatag cgttcgtaat ttgaactcca ggaactaaag gcatcacaca
    20521 tccgataatg attagatcct gattgcttcc taagccaaaa cgtacgctgt agatagcggc
    20581 tatgccaatc aataaagaag ataaaaattc cgataaaaat ttgattctaa aaaatttcaa
    20641 actatataga taagctgcgt atcccaatcc tccaatgact aaagtcacag gtaaatcggt
    20701 catctttccg ccaaataaca ccataattgt tccactgatc actgccgcgc taacgatttg
    20761 caaccagaca ggaaagaaag aacggtctcg gtcgattgct tgcagttcgc agtaaagttc
    20821 ttctaatgtt agctctgaag caacatattt acgtgaaaga tcatttactt ttactacttt
    20881 ttctaaatta atcgaacggt tggtaatttg ttccattcgg atggtcgagg tacgatcaag
    20941 accaataaag agaccggttt gagtgacata gctgaccaaa cggaaatttc cagaagctaa
    21001 agcaattcga ctcatcgtat cttctacccg atacatctcc gcattgcttt ccatcatgat
    21061 tttcccagcg agtaaacaag tgtccagcaa cagatctaat tcttttcccg tcatagcatc
    21121 ttctccaata attgattgat tagttttatt ttactacaaa acataccaga agcgtaaaga
    21181 ataaaaaaac ttgaggaagg aataaaagag gcgtatactg tttttgtatt gcgggtgtag
    21241 tttagtggta aaatcccagc ttcccaagct gatgtcgcgg gttcgattcc cgtcacccgc
    21301 ttaaaatagg ttgtgaaaaa gctgctctgc aacaagtaat aagaacagcc aagcaaaaac
    21361 aacttcttat gtttttaact tggatggact tattaccgca gatgcaagct tttgcgcacc
    21421 gtttaccaag cggttgtgaa atcagtgtat tgtcttgagt atgaagaaaa tttttgaata
    21481 ataataaata ccttacagat agagtggtga ctgattagag acgaggaatc cgtctctttt
    21541 ttgtattttt aagccatctt aaaacaggtt ttattttttg ctgtttctat ttctttctag
    21601 tatttattta taggggaaca tatgttaaga tttagggagc tgttagtaaa ggagtaaaga
    21661 aatgagcaaa aaaacaacca aggcgatcgt ggggatcgtc gctgctgtga ttgcaggggc
    21721 attcggtgtt tccaccacac aaaaagattc agatatcttt cagcagatta caactatttt
    21781 tgattccaca ccacaagctt ctcaagtagc ggatgtgcca gaatatgatg gaaaacatca
    21841 agaaatagaa atcaatggga atcagccgga atttacaaaa gaggatctta gcctttccaa
    21901 aggaacatgg gaaagctatt caaatattga taaattgaac agagtaggac aagccaatgc
    21961 tatgcttgga aaagatttgt tccctactga aaagagagag cctttgtata ttgacccgac
    22021 aggttggaaa caaaagaaat taagtgacgg gcaatggtta tataatcgca gccatttaat
    22081 tggtttccag ctgactggtc aaaataacaa cattaaaaac ttgatgacag ggacacgttc
    22141 gttgaatgct ccatacatgc ttgctcacga aaatgatatt gcggcatata tcaaagaaac
    22201 aaatcatcat gtacgctacc gtgtcactcc tcattttgag gggaatgaac tggtagccag
    22261 gggagtgcaa ttggaagcag aaagcattga agacaaaaaa attgaattca atgtctttat
    22321 ctacaatgtt caagatggct acacgatcaa ttatgagaca ggacaagcaa caaaaacaaa
    22381 aaattagtag cagctattcg aattttttat gaatattatg aaaaaattgt gtaaaaacat
    22441 acattcaaag aaatatagcg tatactttat gaaaagagta cttttgataa agctacagaa
    22501 aaaaggaaaa aaacatacaa atagtgtttc ttaatttatt cggaagtttg gaatatttgt
    22561 ttgtagtttt ccctttcttc gagtaaaata aggttgtaga aggtaagctg gaattacctt
    22621 gcgggaaaga ggtgtagaat catgggtttt acagatgaaa ccgtacgttt tggttttgac
    22681 gatagtcgca aaaaagaagt gagcgaaaca ttagcgattg tttatcgggc tttggaagaa
    22741 aaaggctaca atccaatcaa tcagattgtc ggctacttgc tttctggaga cccgtcctac
    22801 attcctcgtt atcgtgatgc tcgaaatttg attcgccgtc acgaacgcga tgagatcatg
    22861 gaagtaattg tcaaggacta cctctcaaat catggagtga atttatgaga gtaatgggct
    22921 tggatgtcgg ttctaagaca gtaggtgttg ctatcagtga cccctttggc tggacagctc
    22981 aaggagtaga gatcattcgg atcaatgaag cagaagaaga gtttggcttt gatcggttga
    23041 aggaattagt agcacaatat gaagtagacc ggtttgtagt tggattacct aaaaatatga
    23101 acaattccat cggaccaagg gcagaagctt ccatgtcata cggagaaaag atcaaagaac
    23161 tattctcctt gccagttgat tatcaagatg aacgattaac cactgttcag gcagaacgta
    23221 tgctagtaga gcaggcaaat acttcaagag ccaaacgaaa aaaagtcatc gataaattag
    23281 cagctgtgat gatcttacaa aattatttag acggctcagg taagcaatta ttttaataat
    23341 aaaagaaaag aggtaatctt atgacagaac ataaccattc ccacgaccat gaaggacacg
    23401 aacacatcac attggtggat gaccaaggaa atgaaacatt atatgaaatc ctactaacag
    23461 ttgacggaca agaagaattt ggacgtaact atgttctttt atatcctgct ggtgtttcag
    23521 aagatgaaga tgtagaatta caagcttatg catacatcga aaacgaagat ggaacagaag
    23581 gcgagttaga acaaatcgaa acagacgcag aatgggacat gattgaagaa gtattcaata
    23641 cattcatggc ggaagaagaa gaatagacta taaagctagg agaaaccttg atatgatagg
    23701 gtttctcctt tttttatttt ttactgtatg ccatattgta tgccatctag tcaatattta
    23761 agtgttgtac taatttttca acagtctttt ctttcgtttc ttttgccaag tgaccgtaac
    23821 aatcgatcgt ctgactaatt tttgtatgcc gtaatctagt ttgaacttct accatagtag
    23881 ctccagcgtc caagagcatt gaagcatgag tgtgtcttgc tacatgcaca ccgagaaatg
    23941 gaaccatagc tttttcacaa atagtaatca atcttgcata aatagcgtgg tgtgtgatta
    24001 gttctttata gataccacaa aaaatcatag tcggatcagt tacaccaagt gtcagcatat
    24061 atttccgctg attaatctgc cattttttta atatagaaat tgttctttca tctagtggaa
    24121 gaaccccagc actacaatca gttttaggat cactgatttt atagccattt ttggattggc
    24181 ttagtgtttt attgatggtg accgtatttt cttgaaaatc aatgtctgac caattcaatg
    24241 caagggcttc actgatccta accccgctat aaaagagaaa gcgtagtaac gttttatcaa
    24301 agtcgtttcg ataagtttca ggtttgctgt ttaggtaatc aaacaaggtg atcagttgtt
    24361 ctttactgta aactttgacc ttttctttat gcttagtggt ggttctttta ggcattaata
    24421 cgttgtctag tggattagtc tcaataatcc caagattgat agcgtagcta aaaatacgct
    24481 tagaagttga atgaagtacc ttgtattggt cagtagtttc agcccagcta tttacaattt
    24541 gttgggcttt ttttaccgtg acttcattca cacgaagatt tccatatttt ggaagaatcc
    24601 actttctaaa cttgatttca gttttcatga aggtggcttc tcggacagtg gttttatatg
    24661 tttctagcca caagtcataa acttctttaa acgtttcgtt gttcgctttt ttaataccgt
    24721 ttttatcgta gtccacttgc aagcgtgaca aggcaagctg ggcttctttt tttgttttgt
    24781 aacctcgtct acgaacctta tcaggtttgc cagtcatttt gttcattcca agataggctt
    24841 catagagcca aaatttacct tttttagttt catattgctt gaatgttgcc ataatagttt
    24901 tccctccata gtgtagcaag ccgtgggggc ggttggagga ggcagtttat tattattttg
    24961 aatagtgttt atttagctca gtaacaagag ctgtgaactc ttttagaaga tcattttcat
    25021 aaaattcttg atactctttt ttctcatctg tatttttttc tgaatcatta attaatgagt
    25081 catatcgtaa aagcgtattt agaagagctg cgagataagc aattggggta ctcccgtcag
    25141 gaaattcaat tcgagatcct gttttaagat attttattgc gtttgttaaa taaatcttct
    25201 gtgagacagt caggttatct tcttcgatgt tattgatagc attagaaaga ttagaatcaa
    25261 ttatagagtt atttttttca atagtttttt tcaattgctt ttcaagcaca tttcgcttaa
    25321 tttcgggcat catatgcata tctcttaatg tataagatcc gtttaataga taggtagtgg
    25381 atacatctaa tacctttgaa agttgtttaa gcctggtgtt attgggaaga ttatagccat
    25441 tttcccaatt actaacggca cctttactgg ccggtgggga taatttacct ccaagttgtt
    25501 ccattgagag tcccttttca attctagcca gtctaattct ttgtcctgtc tccactgctt
    25561 cggggtattt ttttctagcc acattttcac caccattcat atattttctc tactatatca
    25621 cgatttaaat atgtgtgaaa cataaaagtt caaaaaagta tgttaaaatt cttgcaaatg
    25681 aaaaggtatg atggtatatt gagtttataa gaggtataga aatgtattca agagcctctt
    25741 gcattttttt aaaatctttg tatagaaatg tatgctaacg aggggggata aatatggaat
    25801 taaaaattga caaactcacg cttgatgatc cagcttttaa aattattgac gatcgagttg
    25861 ttcaacaagt agagcaaact caaggaacgt tactttcatt attagaaaaa aagtaccagt
    25921 ttccaaaata tatgaacaag gcacaagctg ctaagtatat gaacgtttca tacaatacta
    25981 tgatgcagaa atatgttcct aacggtttaa agttgattat agttgatgga gtcgtaagaa
    26041 tttcacaaga tgaatgtgat cgctttatgg aagaatacca aaaataaacg caagccgtgg
    26101 gggcaaaggg attaatagga ggaaataata atggaatcga cacaaaataa agcaatcgaa
    26161 aaagtattat caacaattct tacagaagac acagcggcag aattagcaaa cttggaaggc
    26221 aaagcgctag aagaaacgtt tgaatgtttg tacgaacaaa tggactatca aaaattgcta
    26281 ccacaagaac caacggtaag cggagtattg cgtggcttga acgatttagt acaagcagag
    26341 tttaaagaac gactttctat tgaagagtat caagaaattt tgtatcagca agttgagttg
    26401 ttagcgagct tgctagggct tgaactggag gacgttgaat gaaaactgaa atttggaacg
    26461 gacacattat aagatttgtc gatattaatg atgaatggtg ggcagtggcg aaagatgttg
    26521 cggaagcgtt aggtcttaaa caggttacta gagccattca ctctttgcct aaagatgggg
    26581 ttactactag taaggtcatc gattcattgg gcagaacaca agatgtaaac atcatcaacg
    26641 aaaaaaaata tttaccgcat ggcattcaaa agccgtaaaa aagaagccga ggcatttcaa
    26701 gactggattt tcgacatcat caaagaacta cgccagtcca caggacttga aggctttcaa
    26761 gtattccgca tgctagacaa agaacaccaa aaagaagcaa tgactaaatt aagccacgct
    26821 attactgaac caaaaccagt agactacatt aaagcgaatg tgatagccaa taaagctgta
    26881 tcaactattt atggtcattc caaaatggta aaaaagaaag atatgactcc tgaaatgttg
    26941 gttgatcgtg aacctatttt agatgagaca gtagaattaa tgactgttaa agaaaaatat
    27001 gggttgcagt tttcagtgag tgagaaaatt tataatcgtt ctgctgaatt gcagacaact
    27061 taggagcatt aacatgatcg agaacaaaga aaaagcctta tccataacag tcttggcgga
    27121 caggataaag gcaacttaat tgggataaaa cccgatattc tttgttccga ttataccata
    27181 ttaaaaaatc ggagggaacc attatgaaac gaaacttaat tataggaagc attttaatcg
    27241 gcggaatttt cggcttatta ccattaattg caaaaatagc tatctttgca ggattagcgt
    27301 tatatctctt cactgcttat gacgagtacg actatcaatt aagaactgga gggcataagt
    27361 agatgtacaa aacaaaacta ttaaaccaac tcgatagcct tgaattagag gaaattaatc
    27421 aagggatcgc tgaactagaa aataatatcg ggaaaaccta ctttggaaat tcattcaatg
    27481 aaaaactaac agttttgtat gtactcaaaa aacatgcaga acacaaaata atttgtcgag
    27541 aaatcaacga gctaaaaaat cagattttaa ccgcatggtt aaacattact gatatgcaag
    27601 aagcaagggt gaaaacattt aatacatggg taaagtatca gaatcaatta aaaggtgctg
    27661 aatttgttcg ggatgggttg aaatacgaac ttgagcagtt gaagctcatg gaggtgtctg
    27721 aatgatttat accagtattt tgaaaaagga ggagaggttt attggcaaat aattatcaag
    27781 cagcaacgga attacaaaag tcgggatggt caatttatcc cttatctcct ggaactaaaa
    27841 ttccaataaa aggctctagt ggttggaatg atgcaactaa cgattctaat caaattaatg
    27901 aatggtggac ggaagatgca actagaaata ttgggttaat gcttgaagat catcaaatgg
    27961 tggtagttga cctagatcaa cattctgaaa cacgaaacgg cgttgagaat tataaaaaac
    28021 ttgcttcaat gtatgatcca tttccttcta cttatacgga aatcacacca ggtaaaggaa
    28081 tccatttctt ctttaaaatt cctgatggtg taattatcca acaagaaaca ggtgcatttg
    28141 ctgatatctt cgggcgtgat gataaaggta aaagtttaag cgggattgat attatcacta
    28201 aaggagttcc aatagcgcca actatgatta tttcaagaag tgttgggaga gcatatgaac
    28261 ctattgaaaa cgtatctcta aataatattg caccagctcc cgattggcta gtgaacctac
    28321 tgctaaaaaa accgcaagaa ataaagactc aataccgacc cgtagcaaaa acagggacag
    28381 caaaaaaatt agatatgatt gttcaaggcg ctggcgaagg tagtagaaac gatcatttaa
    28441 ctaagttatg cggttggtta ctatggcacg gtgtagataa tcaaacttta gcagaattga
    28501 tttatacagc aaatgcgtac aatgaaccac cgctaaaaga taaagaagtg aatcaaatta
    28561 ttcgatcgat gattaaaaaa gatttgaggg ggaaagctca tggctcataa gcataaatta
    28621 gatagtattt tagactttcc cgaagccagc gagcgtgaag acaatatcat tgaattgaaa
    28681 acctggatgt cacgattaag atgcaataaa gacgatcaga ttaaaagtaa cagtgttgtg
    28741 aatgcagaat tgattttaac caatgatagc aatttagctg gaacaattgc ttataacgaa
    28801 tttagcggat acattcacct attaaaagat tctccttgga tcaatcgtac cgctggagaa
    28861 tgggaagaca gttttgagga tgccttaacg gcatatattg aagaaaacta caatgtagtt
    28921 tttgatgaca acaaaataca caaagcagta gtaaatgtgg ctagaaagaa tgttttcaac
    28981 ccagtaaaag aacgtatcga aaaagttaaa tgggatcaac agcctagact ggaaacactg
    29041 tttattgacc ttttaggggt agacgacaac ctttacacac gagaagtaac aaaacgttgg
    29101 atagtcggat cagtcgcacg tatctataaa ccaggaataa agtttgagat cgttccagtt
    29161 ttggatggac cgcaagggat tggtaagagt accgttccag cattacttta cacggatgat
    29221 tttttcaccg attcattaga ttcgctaggt gaaaagaaag atgattatat gcagcttcaa
    29281 ggcaatgtaa ttattgaact tggagaatta tcttcaatga acaaaactaa aattgaacag
    29341 ataaaaaact tcatcagtgc caagatcgat aaaattagac ctccttatgg tcggaatgtc
    29401 atagcttggg caaggcaatg tgtatttatt ggaacgtcga atgatgggca gtatttgaaa
    29461 gatgatacag gaaaccgccg tttttatcct ttgccttgta agaataagcc aaagatgaat
    29521 ccgtttaaaa caaaggatga atattttctt caagtgttag ctgaagcaaa ggtgcttttt
    29581 gataaaggac aacgaatcta ttttagtccc gatgaagata aagaagtctt agaaattgca
    29641 aaagactatc aagaggatgc caagatggag aatccaatta aagaagcaat cactaaatat
    29701 ttggaaatgg aaatcccgta taaatgggaa gaagctccag cgtgggcgag acgttcttat
    29761 tatcagaact atcctgacag taaatgtaat gagaaaaatt tacagcattt ctataatgga
    29821 gctactatgc aacaagagtt ttatttagtt gatagcgtac tcaccgctga tattttggaa
    29881 gctgtttttg ataaacaagc aaaggattta ttgaatggtc gatcagatgc agaaactaaa
    29941 aagattgcct tgattattag cagtattcct ggttgggaac ggaagcaact gccaaaaaga
    30001 aataaacgac gaggatttta caataagtca aatgcctatg aaaataaaaa tcgagctgga
    30061 aagtacaaaa agtaacgggt agtaacgggt cagtaacacc tagcccgtta ctagtttacc
    30121 cttactgccc caagggatac agcaatttgg taacggggta acaccttatt tccttaaaac
    30181 ttttgaaata gttagctaat atgctgggga ataatgttac tggattttac ccgttacctg
    30241 ttaccaaaac gctagaaccc ttgtccccgt tgggattgag tggtaacggg tacctgttac
    30301 gtaggtgtta ccacgtgtta cgaatcaagg aggaaagtaa atgctcaata ttatttcaac
    30361 aaacaaagca ccgaactttc aatatacaga tgaaatggat cggtttctaa tgaacacgct
    30421 ggcatttagt gtgggattgg taacagaaga ttattccaca tttgatccag aagtattaaa
    30481 gataatggaa gaagaacctg attggctgca ggagtcagta gcgtggtgtc aatcgctagt
    30541 agtgggatca ttggtagata gtggcaacta tgatgataca ggtgagctaa tggatgaatt
    30601 taattgtctg cttaacctat atgatcgagc gagacaacga gagcttacat cgaatgagga
    30661 taatttgttt ttaaacatcc atgataagtt cttggctttg ctactaacag atgatgaatt
    30721 gataactaat ttattggagg tagaataatt atgcatatgg aaagtattag atacttcgat
    30781 ggtgagaaac acattgagta ttcaaacact caagaagatg tagactttat tagttttgag
    30841 gaagataaaa tcactaaagt taattttaaa gacggaacgt gcttgaagat agtttcgcct
    30901 tatattgaat acaaatcaaa atgggaacaa gatgattctg acgaacgaga tcgctttggt
    30961 tggtaatcta tggcagttaa aaaacaatgt aatcatgctg gatgtaaaac attgattgat
    31021 tataggcaga agtattgtgg gaagcataaa gcaaagcaaa cggcaattaa gcgtgaagaa
    31081 agaaagcaaa gcgaaggtaa atattttcaa ttctatcaaa gtagaacatg gcgaaaggct
    31141 tcatacttgt atcggttaaa tcatccagta tgtgaggatt gcctagaaga aggcttgata
    31201 aggaaagctg atgttgtaga tcacaaaatt gaactaaaag atgattggaa tagacggtta
    31261 gatgaaacta attttcgttc tttatgccat gcacaccata attccaagac agccaatgaa
    31321 agacaaagac gagaaaagag taccctttga gttaaggggg catagtgtga aacttactga
    31381 caatcgatgc ctactcattt tggtacaaat aaccgttgtg aaaaggcata agggtaatta
    31441 caaataacga ttacacttgt aatcacaagt gaaaaagtat ataatgaaag taggagattt
    31501 attgaaaaat acaattgtta ttactgataa aacagggaaa cagcgcacca ttgagttacc
    31561 tcattttggt caagtgacca ttcaaatgca gaacggaaaa atcatttata tagataaatt
    31621 agataaagaa aaattctgat cgaaaaacga aggaatgtga gcttaattgc ttacgttcct
    31681 ttttcttttg tctgaaagga ggacaacatg ggacacccta aaatattaca cgatacaaaa
    31741 ggcaatatat caagtgaaga aaaagcaatc cgtgtggacg ctagagaaga attgttcaag
    31801 cagcaaccat taataaatat cacgccccct gactggatgg cagcgagcgc tagaagtgaa
    31861 tggaatcgta tcgtaccaac attaaaaaaa gattatccat tgagtgaagc agattatggt
    31921 tcattggtag cctattgttt agcctttgct cgaatgaaaa cagccgaagc cgagataaga
    31981 aaatcaggta cgtttatcac atgcgaaaac ggagtaaaga aagccaatcc agcggttcgg
    32041 gttcaatcac aagctatgag tgatttgaaa aaacaagcca cctcactagg tatgacctta
    32101 gaatcacgat caaaactagc tttgaacaag gttaaaaatg atgaacccga agacccgttc
    32161 aaagagttga tgaattcatg aatgattaca ttgagaaagt cctatcaggt aagctgattg
    32221 cacccaagaa aattatccaa gcgtgtgagc gtcatataag cgatttggag cgttctaagt
    32281 cagatagctt cccttatgtg ttcgatgaag aacaagccac caaagcgatt aaattcattg
    32341 agttgctacc atctacggac ggtaaagcaa tcaagatgtt aggatttcaa aaattcattc
    32401 taggcagtct ttatggctgg cgtactaaag aagggaatta caggcgattc aatcgagcgt
    32461 ttaccagtat gagccgtaag aatgggaaaa cgtatatcgc aagtggcatg gctgccaatg
    32521 cgttgattat ggaacaagaa ccagcagaag caaggcaagt attgtttgta agtaacgctt
    32581 tgaaacaagc taaattgggc tatgatatgc tgtctaattc acttagaaac gtggtcaagt
    32641 ctagtaagtt tttaagacca caactgaaaa ttatgaactc taagattcag cacttgccct
    32701 ctaattcgtt cgctatggca ctggctagtg aaaccagcac gctagatggg tttgcaccaa
    32761 caaccgcaat tcttgacgag tggcacgaag caaaaactcg taaaacgtac aacgtcatta
    32821 agtcaggaat gacccaacaa aagaacggct tattgtgtgt tattagtacc gctgggcttg
    32881 atttaaacgt tcctatgtac gaggaatact tattgttaga tcgtgtgcta aaaggcgaag
    32941 aacaagccga caggtacttt atagcgatat gggaattgga cgatcccgaa gaaattcacg
    33001 atcaagagaa atggatcaaa gccaatccga tttttgaaag tgaagaaatc aaaaaagtaa
    33061 tgattccaac cattcaagat gatgtgaacc ttgctttgaa acaaaataac cttaattctg
    33121 tattggtgaa aaacttcaat ttatggagac aagcgagtga ggacagctat atgattgctg
    33181 aagactggca ggcaaccgaa gtagaaccac aagatattac aggcaagccc gtttatatcg
    33241 gagtggattt atctaaaaca gatgatttaa ctagcgtttc atggatcgta ccgctagata
    33301 acggcgaact ttattgtgat tcacatagct ttgtagccac caaatatggg cttcaagaca
    33361 aagaaaagcg cgacggtttg ccttatcgag aactagaaaa agctggtgag tgttccatta
    33421 ctcaattaga aagtggaatc gtggactatg accaagtatt tcagtttatt caagatttga
    33481 ttcaagaaaa tgatttggaa tgtatgggga tttgttatga cccgtataac gctaattcgc
    33541 ttatcagtaa agctgaaaaa gccaactacc caatgttaga agtaagacaa ggaacgatta
    33601 ctctaaacgt tccgacccga acttttagag aacaagttta tgaaggcaac gttattcaca
    33661 ataaaaatac gattctcacc catgcagtga acaacgctat tttaaaaacg gataacaacg
    33721 gcattcagat taataaatca aagaacagta acaaaattga tccaatagct gcattaatca
    33781 atgcctatgt gtttgcaatg gattacttca ccacaacgga aggagcgaaa gcagacaatg
    33841 aattttatac aagtgaagaa ttttctttct aattacattc ataccgttct tttacttctt
    33901 ggattggtgt gtgtgttggt tgctatcacc ttactaacaa atgtctacta tggcttgtta
    33961 gcgctgggca tagtgcttat tggggtagcg gtcatgctaa atacagaaca gaagggaggt
    34021 taaaagatgg cattttttaa agcgagacaa aatacaacgg gagatccttt cctacaacat
    34081 gtggtatcaa tccaatcgga tgattacacc accagcttta caagcgttcg tgcattaaga
    34141 aatagtgatg tgtttgcagc cgttcgaatt attgctagtg atattgcttc aagtccgatt
    34201 caattggtca aaaacaatat gccacaagct gataatgaac tggtgaagtt actaaacgag
    34261 aaacctaatt cagaaatgga tggttggcat ttaaagtttg ctttggcggt caatatgttg
    34321 ctaaacggta atagctttgc agagattaaa cgcaacggtg aaaaggtaga agaaattcac
    34381 ttattaccta actcaagtgt aacggttact caattagata atggtacgtt gtcttatcag
    34441 attggtgata aaaaaagacg tgtgaagtct agcgatattt tgcacttcaa atatttcacc
    34501 caagatggtt taacaggatt accaccactt tatgcgttac gtgatgaact gaaaattcaa
    34561 caagctggca accgcacatt gcacaatttc tttactcgtg gtgtcagtgg gtcaggtatt
    34621 ttgaaggttc ataagtcaga tttagacgga tcagcaaaga acgcaattcg tgaaaagttt
    34681 gaagaagcca acggttcaag tagtggagat aatgctctta gaacgatcat tcttgatgaa
    34741 acaatggact acaaaacatt agaaataaac accgatgttt tgaagcttat caattccaat
    34801 gattggaaca caaaacaaat tgccaaggcg tttggtgtac cgattgagcg tttaggcgtt
    34861 gaaaatgaac attctagcac cgttcaaagt aatctccagt atatccaaag tacgctgatt
    34921 cattacttta atgtgtttgt gagtgaatta gatacgaaac ttgaaaccaa tatccgcttt
    34981 aattccgatc agttactaga gactgatcct gaaacaaaag taaaaaatgt attggatcag
    35041 gtcaaagggt cacttctcac gattaatgag gggcggtcga aaatggggct accccccatg
    35101 gatggtggcg atcgtttact agcaagtttg aactttacgt atttagatac gttggagaaa
    35161 tatcaattaa aagaacagga aggagttaca ccagttgaat aatgaagaag aaaaggaaaa
    35221 acggctgaca gaagaagctg agctaacagc cgattctccc aaagtagaga aagaaaatga
    35281 aaaacaacca acagacggca aaactatttc aggctatgcg ttgaaattcg ggcaaccgtc
    35341 aaaagattta ggcggctttg tagaagtgat tacacccgaa gcattaaaag aggtggattt
    35401 atcaaatgtg ttcttattat atggacacga ttattcaaag ccactagcta gtgtcaaagc
    35461 aggtacgttg aaattgaatg tcgatgatac aggattacat tttgaagcag agctaaccga
    35521 cacgacctat tcaaatgatg tgtatgagaa tatctcaaaa ggggttgttg attctatgtc
    35581 ctttgggttc gtgcttggat tagattcatt cgacaaaaaa gaagatggca caattgaacg
    35641 atcaatagat aaaatcaaag cacttaatga aattagtgtc gtgaccgttc ccgcttatga
    35701 ttcatcaaat gtccaagtaa ataagcgttc ttacgaatcg tttatgagta acaaccaagc
    35761 aaaacaaaca aacaatagct tagaatccac ttctaaagca caaaaggaga gtaataacat
    35821 ggaaaaaact ttaatcgata acgaaaaaac agaattgcgt gggtatgaag aatatatccg
    35881 ttcacaaggc gaagtgcgtg atggagtcac tactgtaaat gcagcggcag ttgttcccga
    35941 agaagtaatc ggtgaagtct ttgatttgaa acgttcaaat tataacttag ctcaatatgc
    36001 aacagtaaaa acagtatcaa atggacaagg taaatatcca gtagcaacta accaacaagc
    36061 agtgttagca acaaaagctg aacttgctga aattggtgat attgacgctg aaatgtttac
    36121 ttcagttgat tataaagtag aaactcgtgc tggtaagatt gccttatcaa atgaggttgt
    36181 ggaagattca gcagtgaata ttgtacaaga ggtcaaagat cagttagcga aattggtaga
    36241 aaacactgac aataagcata tcatggattt attaaaaaca ttcactaaga aaacggctgc
    36301 tacgttggac gatttgaaac aactatacaa tgtggcatta gacccagcat taaataaaat
    36361 ggtaattcta aaccaaagcg gatattacca cctagataca ttgaaagatt cagatggacg
    36421 ttacatttta caacccgatg tgacagcacc tagtggtaaa tcattattcg gtatgccagt
    36481 agtattgatt gcagatacat tgtttgccaa tcctaaagct ggaacattcc ctatgattat
    36541 gggggatatt gcacaatcta tctttgttgc tcgtagaaat caagtaacga ctcaatggga
    36601 aaaattcgat tactactcac aaggacttgc agtgatcgtt cgcaacgatt ataagaaaat
    36661 tgatcaaaat gcttcagtgt atattgagtt tacgccagtt gtaacaccaa aagtatagaa
    36721 aaatattggg cggcggttta tcccactgcc tttttttcat taggagagat aaaatggtaa
    36781 ttttggatag cataaaaaaa agtatgcgga tcgatcacac tattgatgat gactttattc
    36841 aacaattgat tgatacagca ggcgaatata taaaaagtgc tattgatagt agcgcaacgg
    36901 ataaagatat ggataattat cagcaatttg atttggcggt gtcattactt actcaacatt
    36961 ggtatttgaa ccgtcaagaa gccagcagcg aacggatacc agtaacggta caagcattag
    37021 tacaacaaat gagaggtgct tattatgcca atcattaaga atgtaaatga attgactgag
    37081 agagtcaagt ttaaaaagac aaaacgggta aaagatgaag atgggcagat ggtggatagt
    37141 gaagaaactg tatttgaatg ttgggctaat gtgcgttcac aaatgctaaa agacgtgctt
    37201 gctagtgtag gtaccattct tgaaggaacg ttgacgttta ttattcgata cgatcaagat
    37261 tatgaactaa caaacgatat gaaagtagct tggaaaaaca agagttataa aattatttca
    37321 atcaatgaag gaacggcgtt taaagattat acaacgatca tagctaaaca gatttcttag
    37381 aatgattaca cttgtaaacg ttgtgggtat ttagtataat taaggtagta aatgaagggt
    37441 tagctgcctg aattttgcaa gacttagcta gtcgaaatct attgttggac tgaaaattgt
    37501 aggtgtggtt gcaaacatgc tggactaaga aagatgatag atgatgaacg ttcaatttta
    37561 atacaggcat gctaggactg attggatggt tttggcaagc tgattgtgta gaaatcaaag
    37621 aacacgtctg ttgtggacgt gttttttttg ttataattca aaagaggtga tttaatggac
    37681 aaagcagagt ttacaaattt ttattggaag tattatctac atttagaaaa tgagtttatt
    37741 aataccacag attttgtgat gctcgatgag aataatttca aaacattctc tattgaatat
    37801 caaaaactac tactagcgat tggttcggag tgtgagatta ttttcaaaga actttgcgga
    37861 tttgatagca gtagcaataa aagcataact gattttaaag ctgttattaa atctagcgaa
    37921 ttaatgtgtt tggataatgg tgttagggtt atgaattctt taacattgac ttcactaaaa
    37981 ccttttggag atgggtggcc tgaagataca ccagattggt ggaaaatata taacagagta
    38041 aaacatggtc gttcttcaaa ttataaggaa gcaaatttgg aaaatgtgtt gtatgcttta
    38101 gcaagtctat atttattaga ggaatatttg cataagaaag taatactaga aggtgaagta
    38161 gactttataa gtccagagtc aaatctattt acgttatctt gggaaagaaa acattcgtcg
    38221 atgcaaaaag tgacgtttga aaaagtggat tcaaattatg tagctccact cattgatttt
    38281 aaagaacaag aagaaattta aaaaccccca cggcttattg aagtttttgt atgccatatt
    38341 gtatgccatt tggtttaaat cgcttgaaaa atagtgatag ataactctaa ttcatttagt
    38401 cataaacgct gttttgataa cctttatagt tctttgatat gggtttctca ttcatggcgg
    38461 aagaagaaga atagaacaaa agaatgaatt aataagatat aaaaaaaggg attgtgacaa
    38521 acgtcttgtc acaatccctt tttccaaata aacgatgttc aatcagctgc ccctcggcat
    38581 tcgctcgaaa aacggcgaaa tatgaagaac tattttgcca tttgtttctc taataatcag
    38641 gtcaaaacgt tgacttcaca gtctcttcct taaacggtgt tctcctgcca actcctcggt
    38701 cacaagtcac aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact
    38761 cggagctgaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag cttgcatctg
    38821 cggtaataag ttcaaccaaa cgaaaattat gaggagctat ttttgtttgg ctgttcttat
    38881 tacttgatgc aggacagctt tttcacaacc tcttttcgtt tagcactaaa tttccttaaa
    38941 aagtagaaaa gctttcgaag cctcgtttgt ttcttttact gtataataaa agaaaagagt
    39001 aacaatcgct aaaagatata aacaagagaa aggaaataca agatgattgg tctgatttat
    39061 tttgtggtga tcgttctggc gaacacagtt ggcgctattt ccgggatggg tggaggcgtg
    39121 ttgatcaaac cgattcttga tcttatcggc gcccattctg ttgcagggat ttctttttac
    39181 tcaacagttg cggtattcac gatgtccatt gtttcgactg tgcgacaagt atcaagcggg
    39241 aaatcactga attggcagat tgtcggttgg gtatctagtg gagctgttgt tggaggagta
    39301 gtcggcaaca ttgtattcga agttttttta cagttatttg aaaacgagaa gcatgtgcag
    39361 ctgatccaaa tttttttgac ggtattgacc ttggtttttg cgttcttcta caccaagcat
    39421 catcagccaa aatttcattt gacgtcatgg acttggtacc ttatatgtgg gggggttctg
    39481 ggctttttag ctagtttttt aggtattggt ggtggaccag tcaatgtttc tttgttgatg
    39541 ctgatgtttg cattgccaat aaaagaagca acactatatt ctctatctac gattttcttt
    39601 tctcaactgg caaaattggt aacgattgct ttaacaagta gctttatgcg ctttgattta
    39661 tctatgttgt tttatgttat ccccgctgct gttattggag gtttgtgggg agcaagattt
    39721 agcagggttt tgtctccaaa aaaagtgacc tttatttttc aagtaattgt cctcgtggtt
    39781 cttttgatca atctttacaa tgctttcgtc atcttacaaa cattaggaga atctagacta
    39841 acgcaagtca aaatcactga aacatattgt tctaaatgaa aaaaggagta tactgtgata
    39901 aaaaaattca tttaaaaaaa taaaaattgg ctggtgattt ggatgttgcg ggaaatgcgt
    39961 ttaggagatg cagaagcact acgagaaatc aatgcggaac aattaggata tgatgtacca
    40021 ttaaccgtta cggtgagaca aatgaagaaa ctgttgaagg agccagaaaa aaattttttc
    40081 ttggtctatg aagatcctac ttctaaaaaa gcagctggct atgtacaagc ggagtcctat
    40141 gaatcgattt tcagcgatcc gatgttcaat attatggcac ttgctgtgtc taaacatgct
    40201 gaaaaaagag gaatcggaaa ggccttgatg actggcgtag aagaagcgaa aagacgagga
    40261 atatcagcaa taagacttaa ttctgcagaa tacagagtag aagcacaccg attttatgaa
    40321 cgcatcggtt atcattccga taaaatgcaa aaaagatttt tgaaaatcct gtaaattttt
    40381 atcaaaaaat acctggtcat caagccgata cttgatgatc aggtattttt tttgactaag
    40441 aaagtaattg catcaagaaa cctgagatga tcaaaacgat ggcaccacca agccgattgc
    40501 ccatttgagc aaaagcaata agttccatcc gattagctgc agataagaca gctacatttc
    40561 ctgtaccacc catcgagttg ttgcataagc cagcagtaat agttgattcg attggatata
    40621 aaccaaacaa acgaccgaca aatcctgaaa ctactgaaat cgcgataaca cttgtcaaac
    40681 ataagacaac aaattgccat gtcaaagcag aagccaatac atttagattc aataacgcaa
    40741 taccgatacc ggcaagaaca gctgctgtca agtttttgac aatcaagttg ttgaacatga
    40801 tagctgcatc ttcgtaatat tctggtaata ggtttgcgat tttacagata acaacgataa
    40861 tgatcatgaa tgcgtaagca tggactttag gaacaaaata gttacagatt tgtcctaaga
    40921 tgaagaaagt caatgatacc atcaaaccga cacctaattg tggaatggaa ggttttacat
    40981 ttctaggttt tccatcgcca aggtcgcctt tttccattaa tttaccatta ccgttgaatt
    41041 ttggcattga ttgtcccgca cgagcaacta acgctgcacc aatgatagct aggacatttg
    41101 tcattgctgt cgcaggaatc aggcgggaaa tgatagaaga agcgtccgta cctaaaacat
    41161 ttgcataaat cgttgataaa ggtacagctc cggctccaac accaccagcc attgcgggca
    41221 atgagatata taaaacggaa tttgcaaaac catttccgat aagcatacct acgagtccta
    41281 cggcaaaaaa ggagacgacc attgaaataa gtgcaacggg aataaaacga acagaagctt
    41341 tcatcaatag attacggttc ataccaagga tgctaccagt aatcaaggca gcaatataaa
    41401 agtctaaaaa gcccatattg ctgacaaagt tttctgtgga ttttactaca tactcaggta
    41461 agatcccaaa tgtagcaatc gcggcagagg caaagatcgc aaaaacggag ccaccaccta
    41521 aataactttt gacgataggg atttttgttc cgataaaatg aaataaatta ccgaaaatga
    41581 ccaaaacagc gattgggcca atcatattcg tcggtaattt tcctaaagcc attgcaatga
    41641 tcaggacaag gaccatgatc aaataaaggg gcaaacttac gccggtaatt tttgttgacc
    41701 aaaacccttc ttttttcttt tcctcaactg cagttgcggt tgttgttttt tgtgccattt
    41761 ttacagatcc tttcttattc tgtatcgtat gaaaaaagcc aaagagaagc ggcaaaagag
    41821 cagtagttgg ctggctgctg ttttgccgca gtcgcttttt tctttttaga cgtcatacaa
    41881 agtttctgta tgttctttta ctataacaat cttaatcttc taaagattta tattcaggaa
    41941 cccatttagc agattcaaca gcttctttga tatccgtgat ttcttcacga ttcaattttt
    42001 gtgcaacaac tgattttgcg acagtttcag caatcgtttg agagaattca gtgatttttg
    42061 ctactggagg caagatagct gctcctggtt ttgttacatc aacgattccg cctaatgctc
    42121 gactagcttg agaaatgatt tcagcattta ctcgtgtagc tgttgaagca atcaaaccaa
    42181 gccctaaacc tgggtacatc aacgcattat ttgcttgacc gatttggtaa gttacaccgt
    42241 tatattccac atcagcagca gggatacctg ttccaactaa agcttttccg tccgtccatt
    42301 caatcaaatc ttttgctttt gcttctgcca attttgttgg attagacaaa ggcatgatga
    42361 ttggacgtgg ggtatgagcc gccatttctt tcacgatttc ttcagtgaat gctccaggtt
    42421 gggtagaagt accgatcatg atcgttggat ggattgcttt tacaactgct tctaagtttg
    42481 tcaattcatc agcattagtg aattctgagc gtttacgagc gaatgggatt tgaccaggag
    42541 tcaatccttt tgtatcttca aacaataaac cctgtttatc tacttgatag aaatgttggc
    42601 gtgcttcttc ttcaggtaca ccttgacgga tcatttcatc taaaaggatg tttgcgatac
    42661 ctacaccagc agtaccagca ccaaatgtca agatcgtttg atctttcaat tgctcacctg
    42721 atatattcaa tccacctaat acaccagcta gtacaacgat tcctgtgcct tggatatcat
    42781 cattgaaagt agtgatttta tcttcgtatt tttctaagat cgttgcagca tttcctcgtc
    42841 caaaatcttc ccaatgtaat aacaattctg ggaataattc agtagctgat tgaacgaatt
    42901 gatcaatgaa ttcgtagtaa gtttcgcctt ctacacgagc atgacgattc cctaagtata
    42961 atggattatt caataattct tcgttattag taccagcgtc gattgataca ggcaagactt
    43021 gtgcaggatt gataccagcc gcagcagtgt agaccatcaa tttaccgata gcgatgtcaa
    43081 caccgttcac accccagtca cccataccta agattccttc tgcatctgtc acaacgatca
    43141 aacgaatgtc gcgtccatct gctgcgtttt tcaaacttgc tttgatattt tctggttcgt
    43201 cgattgaaag aaatgcagca tcttgtggtt tcaagaaaat ttcgttgtat tgttcgattg
    43261 aatccgcaac gacaggatca taaacgacag gcatgaattc aactaaatgt tcgcccatca
    43321 atttataaaa caatgtccgg ttcgtattga atagattcat taagaagata cgtttttcca
    43381 aatcggtctg tttgcttaag tattgaccgt aagcttggac agattgttgt tccaatgttt
    43441 ggacagtact tggaagcata ccagtgattc cgtattttgc ccgttcttct tttgtaaaag
    43501 ctgttccttt atttagaaaa ggattattta ataagtttag ccctgtaagc atgaatgatt
    43561 cctcctaagt tttaatacaa acgaagtata ggagtcagat gaggatatag tcaaaaagag
    43621 caggtataat aaattttatt tatatgagta atagagtcaa aagaaatcaa aatagcggta
    43681 tttgttcttt ttatatcaac aatccttgct tgattactat gcgtatctct tatatgctaa
    43741 agaaagaaaa gtagaagaat aaaggatatg ttagatagat aaatagaatc tctgaagaat
    43801 aaatcacgtg atcgtgacga taaaatacac ttacgtcagg tgataaaaaa tacataagta
    43861 gaaaactaag aggaggaaca gaaaatgaaa gaagttcaat tgatcagaaa aagcgaacta
    43921 tcagaaggtg gttgcaatgc atgcggcgta gtagaagcaa caagctatac attaaagctt
    43981 ggtgccaaca aagcgataat ctctgagttg acagtaggcg gtcttgtgga cagtctggcg
    44041 cttgctgaag gttttattgg agaagatatt tatgagatgt tttcagaaat ccgtcagtta
    44101 aaaaaagggg aaaactgcat agaggttcat catgagagcc cgaatgtgag attcaaacgt
    44161 ggtgataatg agatgatctt caataatcat gtgtccgatc acaccgagct atatggaatc
    44221 gtgaatcaaa tactaactga gctttttgga ttgggacctt atgcgttcaa ggaagaaaac
    44281 ggtaatccaa aattaaacga agaatggcaa gaaacgattg aaatacaaag aaacaacccg
    44341 cacttatttc aataggcatc cacagtattt cgtaaaaggg actttgacac gacttttgtc
    44401 aaagtcccat cgttttttat ctatttgtgt tctaaacatt tctttcagag tagtaacaac
    44461 tggaatgact gaaatgtaaa cagattattt gcctgtatca atacggaaga gtaatataaa
    44521 gtgtgtaaac gctatcatat tattcattca attttttata gagggaaggc attgaaatgg
    44581 aaaaaacgta tcaagcaggt acaaatgaag gaatagtgga ttttatcaat atggaagatt
    44641 tagaaatagc cgcttcacaa gtgattccag ctggtggtta tggttacatc agcagtggtg
    44701 ctggcgactt atttacttat caagaaaatg aacgggcatt caatcatcag ctgattattc
    44761 ctcatgtatt aaaagatgta gaattacccg atacaactac acattttgac aaagaaacgc
    44821 tgactgcacc aatcatcatg gcaccagttg ctgctcatgg cttagctcat gtgaaggcgg
    44881 agaaagcttc tgcaaaaggt gtagcagatt ttgggacgat ctatacagca agttcttacg
    44941 cttcttgtac attagaagag ataagagaag caggcgggga gaaagcaccg caatggtttc
    45001 aattttatat gagcaaagat gatgggatca atttggatat tcttgaagtg gccaagcgta
    45061 atggagcaaa agcaatcgta ttgacagcgg atgcaacagt tggcggaaac cgtgaaacag
    45121 accgcagaaa tgggtttacc ttcccactgc caatgccgat cgttcaggct tatcaatcag
    45181 gagtaggcca aacgatggat gctgtgtaca aatcttccaa acagaagctt agtccaaaag
    45241 atgtagaatt tattgctgct cattctgatt tgccagtcta tgtgaaaggt gtccagtcag
    45301 aagaagatgt ctatcgttca ttagaatctg gtgctggcgg aatctgggta tctaatcatg
    45361 gtggccgtca actagatggt ggtccagcag cgtttgattc gttgcaatat gtggcagaag
    45421 cagtagataa acgtgtacca atcgtgtttg acagcggtgt acgacgcgga cagcatgttt
    45481 tcaaagcaat cgcatcaggt gcagatcttg ttgctattgg cagacccgtt atttatggat
    45541 tgtctttagg aggaagtaca ggagtccatc aagtgtttga tttctttaaa acagaattgg
    45601 aaatggtcat gcaattagct ggaacacaaa cagtagaaga tatcaaaaaa ataaaattgc
    45661 gtgagaatcg ttttatttaa gaaacaaatg acgaaaaaaa taggagtgcg gcagatgatg
    45721 ccgtactcct atttttatag ttcaaggatg tcactaatag aataatttga aacgaaaaaa
    45781 taaaattgaa atattttttc atacttaata aaaaaggaag aatattcatg taaacgattt
    45841 tattgttttt ttctctttta tacaaaaagg gtcataattt caagtaagcg gatgaattca
    45901 tgaggatttt ttgcatactg tctctactat acttaggtca ctaagaaaaa ggagtgattt
    45961 attatgaaaa agaacatgat tgggacatta ttactttcaa cacttcttct aggcggcttg
    46021 gcaacaccag cttttgctga gggtcaagcg acttcaaaag gagatattac ctttactgaa
    46081 ccaacaaata ccgtggagcc tcttaaccct actgatcctt caaaaccagt agagccagca
    46141 gatccggaaa atccagcaac tggccagaca ggtcctttaa cactggatgt agttcccgaa
    46201 ttgccatttg gtacacacga gattgaaagc ggaacaaaaa cgtatcaagt agacgcttca
    46261 aaaaacgata caccttatct ccaagtatca gatcgtcgag gtgtaggtgc agatggccaa
    46321 gcacaagggt ggaacgtgac agtttcggtt tcagattttg taaacggttc acaagttttg
    46381 caaggcgctg aattagattt tggaacatcc actgttaaaa gtacgtcaga taacgaatcc
    46441 actggaccaa ctagtcaaac tgtaactggg ttgagtaaag caagtgcagc gacaccaata
    46501 tttacagcag caaaagatca aggtctggga acatggcttt ctgtttatga tccagcaaat
    46561 atcacactga aagtgccgaa agcagcagct ggaacattca cggctgattt gacttggaac
    46621 ctcgtggctg gcccagtagc atagagtatg aaataaattc aacaaactgc ggtgaaaata
    46681 ctataggtga aggatttttt accgcagttt ttgttgaaag gatgtggtgg acaatgaatc
    46741 aaagtaaacg actgtttgtt tcgttttttt ctattttaag tctatttttc atttttccgt
    46801 caatcagcca agcaaaggat tcagcaggag actttggaat caaaccggta tttccagaga
    46861 accaaataga taaagcaatc ggttattttg atttattagt ggcaccggaa caaaatcaga
    46921 cactagaagt aattatcagc aattcttcag atgaagaacg aacatttgag gtctctgtca
    46981 atccggcggt aacgagtgat ggtggaacga tcgattatag tcaaaaaaac ccaacgttag
    47041 atgagacatt gccgtttgat gtccgtgacg ttttgctcat tgcgaaaaag gaaatcaatg
    47101 tatcagcaca tgcagaaaca actgtaccta tagaagtgaa aatcccagca aaaagcttta
    47161 agggacgtgt attagcgggt attcatgttt cacctaaaga agaagctgaa acagaaaatg
    47221 ctacagaagg agcacaaatc aaaaatcgta ttgcttataa tctcgcagtc gttttacaag
    47281 aaagtcaaga gacgatagaa ccggatctga aacttttatc aggagatttg gacgaggtca
    47341 acgcaaagcc aactgttcag cttcgtttcc aaaatcctca gcctaggatt atcagcaatt
    47401 tgatatttac gagcaagatc ttttatgaaa atcagcttta tattgaaaat acgtctaatg
    47461 catttttagt tgcaccaaat tcgaattttc acttgaatct tgatttagct ggtgataaag
    47521 caaaagccgg cgattaccgc gcagaaatca tagcaaaatc aggcgacagt aatgaatggc
    47581 gatttacaca aaactttacg atcaaaaaag aaaaagcaca aaaagtaaat gaaaattccg
    47641 tttttgcagt acaagaacaa tctttcccat ggctttatgc aggattggtt gctgcagttc
    47701 tagtgttatg gttgattttg ctgtttttgt gggtccacaa gaaaaagaaa aaaggagaaa
    47761 caacatgatg aggtgatttc gttgaaaaca tcgatcttat tgggagggat tattgcaagt
    47821 ttgctgttct tcagctggca agagccagtc caagcagata ccaaaagttc aggtacgatt
    47881 acattttatg atctaagtcc aattccgcat ccaagtggtt cagtcaatgg cgcatcaaaa
    47941 cagcagacac agctaccgat ttataaagaa tctaatcaac atggcgtatt cccggagacg
    48001 aatgaacaaa agatccgttt tctttatttg atcattggta tagaattatg gcttcttgcg
    48061 tggttgttgt tatggagaag aaaggaaaag gaggatgaac atgttgctac aaaataaatt
    48121 ttcatttttt cgctccttct tttttacttc gctttttgtt ttaagtatat ttatcttttc
    48181 accttcggca gaagctgttg gagcaacgcc tcctgccatt tcttatgatc catacacgta
    48241 tgaaaatatc gtacctccaa atggtggacg tagtgcagtc aatggcggga agcttgtcca
    48301 actaactggg gctaaccaat tgcctaaatt gactgctact tatttgaacg atggttcaaa
    48361 tgatccttgg aaaggtggca cggtccgttt tcattgggaa agccatgtga aactaacaaa
    48421 tgtacttgat ttggtgaata tcactgcaca aggatttgca tttgtggcga tcatcaattt
    48481 gccggaacat gtatcaagca gtgatgtatt aaaagcaatt gactggaaca acgcctactt
    48541 gtatattgcc ggtgccccca tcaaggttac tagcggaagt ttattatcag caggaagcca
    48601 taccttgcgt tttgggatgg ggtcgtggga tacaaatact ctattgacag atattattgc
    48661 tattattacg cgaggcgaat tcaacgtgga cgatatcccg cttcaatttg atgttacagc
    48721 taatgtcgcg aagatgacag aaaacggaga taaatatgat acgagtccaa acaaaatttt
    48781 gaccaagggt aaattacaac cttctatgtc taaaaaggcc gttttttctg ttgattttta
    48841 tgattcgaat aatattatcc aagatcgtat gttcaattcg attttagctc cagctgcagg
    48901 tagattttat cctaaattaa ctggcggaaa cttgaatcca ggagcatatg cattttctgc
    48961 aagtgcagag ataaatacgt ggaatagtta catctcacca agtgattcaa caggaaaata
    49021 taatacattt gctgatacca cagaatacgt ggatggaaat aaccagaacc ttccaggctc
    49081 taccaatagt cggacattga cgatggggct gactgaagga cgttttgatc agttgccatt
    49141 tagtcgcttt gatcgcgtag tcaactattt tacaggaaaa aacgttacac aaggagcaat
    49201 actgactcat actccagcaa gcacaagtgg attgaatcag acagccaata tcgtgtacag
    49261 tgggaaagat gcgcaaggcg gattgctttc tcctgttgct ttaaaggtat tggagcagta
    49321 caagctagac ggaacggttt taccaacaaa cttaacagcc gatagtcagt ggggaaagct
    49381 cctttcttct gcatcgtacc aagtcacggc caattggtca gcaactgaac taaaaaaggg
    49441 ttcgatccat tacaggcttt attcgaaagg aacccaaaaa ttagcgacag gatggacaga
    49501 tcaacttttc caaacgatcc aatcaaataa cgggggtgta aatacagcta cttctacact
    49561 acctgcattg gcttcagggc aatattattt tgattatcgc ttggtggacg atgtacttac
    49621 taacatgtat ccgtcttttg cgtacaaatg gcagtctgaa caagctggaa tcaccactct
    49681 tcctcaaata acagtggcta attttccatc tgtgagtgca gatagtcagc taaaaaacct
    49741 tagcagacaa cctgagacag gagatcccct gtctgctcta tcaggagata cgattcaaga
    49801 aaatgtgaca ttcacgttaa caaaatctgg ggatgcgata accgacaaaa aaatcacgat
    49861 ctcacttcct gaaaatacta cttatatcaa tggttcctta aaattaaatg gtacaactat
    49921 cgcagatacc ggcattcaac aaggagtttc tgtcccagca gatctattga gcaaaatagg
    49981 agataccatt catcttactt acaactatca attaaatact gtagatactt ctttacaatc
    50041 cgtcagcata ctgacaaagg cagctgtact ttccagcaat attacattag cagatggtgc
    50101 taagcttcct aacccagttg tccaaacatc tgctaaaaca attcttgtcc ccaaacaaga
    50161 attaacatta gttaatgtac cagatgattt tacatttggt aacgatctcc ccaaaccgtt
    50221 gaagacaagt tattatgaat caaaaggcga tttttctttt gatgtgcggg atactcggct
    50281 tccaagcacg tctccttggc aactgacagg tacattgact tctttattta agaatgatca
    50341 aggtcaagaa ttaagcggaa caaaattata cttcaatcat tctggatcaa agcaattgat
    50401 tcagcaaggt caaaacacgt tgatttatga gagtgatgga acagctaaag gagaggttct
    50461 cgttgacttt cccgataccg atggtttact cttagaggta aatagcagta cgaatgctca
    50521 acctggcgca acgtatcaag gcatggtcac atgggaactg acagctgggc caaccagcta
    50581 agtgattaga atttgtatgg atctgtgata actgtcacag atcctctttt tcaaggatga
    50641 gaaggaaact tctgctgtta aaatcagaaa tgcttgaagg agtggacttg gaacttttca
    50701 gtttgcttga aaaaaatgaa caacggcaaa tcaaactttt ccaactttta ttagaggaaa
    50761 aaggagaggt ttccgttaat aacgtactta cccgcttatc agttgatcgg gctaccttga
    50821 aagaagatct gttttattta caagctaact tggaaaatat caaacaattg gaaattgtta
    50881 tccaagaagg gaatatttta ttaatcagac atggaaatat agcagcagtc gatgtttatt
    50941 atcattgtta cttgcgctat tctacaaaat atcagatact tctttatctg ttggaaaatg
    51001 gctcatttga tcggcagaaa cttgtcatgg aacttaatat cagttcggct actttgtctc
    51061 gcagagtgaa ggaattaaat gattctttaa atgagtttca tctgaaaata aaaagcgggc
    51121 ggctgaccgg tccagaaagt caaattcgtt atttttattt ccaattattt tggtttggac
    51181 gtccctatct tgaaaacaaa aaagaataca gtgatttatc aggaaacact tttttagatc
    51241 tgctgcaaaa agagtttagt tttgctatta cagaagaagg acgattgaaa tttgaaatat
    51301 ggctttccat tatgaagaag cgaatgcgct gtacgaaaaa atccaaacca gattatagca
    51361 atcttcctgt tggattgttt caaacgacac aatttttatt ggacctccat acatttttga
    51421 cccgttactt ctttcaatat gcctttcctt ggaatcaaaa tgaaacgttg gttttttatc
    51481 ttttcatgat cagtaacttt acattagata cacataactc gcatgtccag gagtttttga
    51541 taagcgaaaa atacaaagac gacgtagtag ctcaattgaa tcgaacgttt aaacaatcga
    51601 tagaagaata tttctctttt tatgtttttc cagaagagat ttcgcaaaag attgcgttga
    51661 cagtgatgca aaatcacgct cattttgcct attttagagg atggatgaat atttttggcc
    51721 aagaaggatt aaaacagcgg ttaactgaag tagaagataa acaattcctg gttttatgtg
    51781 aaagattggt cagacaaaca tttgctattg ttgattttac tgaagtagac gaggaaaatg
    51841 cccgctttga attatttgct cgctatgcat ctattttacg gacgctgatc aagcagttag
    51901 aattacgtct gcaaattgct tatgatcttc cttatgaaag gctaatggcg gatatcgtga
    51961 aagaaatcat ccaggaacaa ttgctgaata cgatcaaata tgatttagtc gaatacgaga
    52021 aagaaaagca gtatgatatc attttgacta gccaattgaa agagtacccg tcacagaagt
    52081 ccgctaaagt ttttgtattc actagcaacg aatataatta tgatttcccc tatttgaacc
    52141 aatttttaaa agaatgttat ttagaaaaat tgaatttaag gatgaataaa acatgaaaat
    52201 cgtgatcatt ggtgcttccc atgaaggcat cacagctgcc ctaaatctaa gaaaacttca
    52261 accgaaagca gaagttttac tgatcgatga agatcataaa gatggactag gctatgtttc
    52321 aaatggaatc aacctttatt taaaaggaaa gatacgtagt ttagctgaag ttgcccataa
    52381 catgcggact cttcaagaaa gtggggcaaa actgatcacc gaatggagag taactgaact
    52441 tgatccagac aaacatcagc tcatcctcgc atccaaagaa ggaaaaaacg agaccataac
    52501 ctatgataaa ttaatcgtag cgacaggttc ctctcctgtc accttgtata aacaaattga
    52561 agctgaaaat gtctacacct ataaaaatct agtgcaatct aagcaagtac ttgccgcttt
    52621 aaaagaagca aaagaagtag tgatcttcgg tgctggttac attggattgg aattagcaga
    52681 tgctttgaga aataaaggat acattattca tctagttgat tatatgccga atgttttatc
    52741 aagatatttc gataaggata tgatcaacag ttttcaaaat cagctgcaaa ctaagcaaat
    52801 aaatttttat ccaaatgaat tcttgatcga ttggaaaaaa tcagaagaga aagtagtatc
    52861 cgtacaactt ctgtctcaaa caataaaggc agatatggtc attttttctg cccaaactcg
    52921 tcctaatacg acattgttaa aagaaaaagt ggctttgtat gaggatgaga ctgtgatagt
    52981 caatgaatat ctgcagacaa gtgatccgga catctatgcg attggagata tcgtcccagt
    53041 cagttttgac aaaaataaac ggcatctctt tttaccttta gttactcgag ctgtacatat
    53101 ggcaagagca gtagcgctaa ctttgagtgg tcagccaacc gcttacgacc tcagacaaaa
    53161 gataacagca gcagtcatta tagactactt tttaggaact gtagggctga cagaagatga
    53221 agcgccattc ttggaacaaa ataccggttc gtgttcagga gaatttgatc tatttccaca
    53281 atacgacgaa gaaaacaaaa cagtaaatgc aaagctgatc taccatccgg atacattaga
    53341 aataattggc ggacagctga taagccaaga attcttatta tcagatttga acctgcttgc
    53401 ggatatcgtg aaacacaaaa ctacgattcc acagctagca gttgaggatt ttggatttct
    53461 gtctgaatac acctctcgtt ttcactacct caatgagtta gcttttaaag tactagctaa
    53521 gagagcaaat agcaatagag tagataaaag actgtgagaa atatcacggt cttttattgt
    53581 gtggggatat aataaatatt aatcaatcga gaataaaaaa ttcatataat gaaatagcgg
    53641 aagaattttt tgagcataat attcgccaaa aatttatcaa gtgataaaat tgttgtataa
    53701 aatgattatt tcaggaggag gagttaaatg tttttagcat taaatgaaat tatgcattca
    53761 aagttacgtt atgcgttagt tgcaggggtg atgtttttga tcgcctacct ggtatttttt
    53821 ttgactgggt tagcttatgg cttagctcaa gataatcgaa cagcagtcga caagtgggag
    53881 gcagattcga ttgtgctttc gaaagatgca aattcaaatc tagggatgtc catgattact
    53941 aagaagatag cagaagaagt cgaaggtggg aaagtggcgt atcttgccca aacacctgga
    54001 gtcgtgacta gtaaagacag tacagaagaa ggaaaaatca atgttagctt cttcggtatc
    54061 gataaaaatc aattcatcat gcccaatctt gttgaaggaa aagcttttga taacgatgat
    54121 gaggcagtag gcgatattag tttgaaagaa gaatatggtc tagccatagg agatacagtt
    54181 aagttatctg gtagtgacaa gacgtttaaa ctaactggct ttacggatca tgccaaattc
    54241 aatgtctcac cagttctcta tacaaccatc aatgcttacc aagaaatccg cttcgaaaaa
    54301 gaagatacaa gtgaaaatgc acggattaac gcaatagttg tcagaggtaa aatcagtaat
    54361 ttacctgaag atttagaaca gatcaaaatt tctaaattca tcaatgaatt acctggatat
    54421 aatgcgcaag tattgacttt tggatttatg atcggatttt tgatcgttat tgcggctatt
    54481 gtgatcggga ttttcattta tgtactgacg atgcaaaaga ttaatatctt tggtgtaatg
    54541 aaagcacaag ggatcacagg aggatttatt gcgcgttctg tagtggcaca aacattcatt
    54601 ttatcatttg ttggtattct tcttggtcta ttaggaacag tgggaacatc ccttgttcta
    54661 cctgatgcgg ttccattcca aagcaattgg ctgttttttg gcgtcattag cttgttgatg
    54721 ttagtagtag ctgtgttagg cgcactattt tctgttcgga caatcgtaaa aatcgatccg
    54781 ttgaaggcaa ttggataaga aggaggaaga acatgaaagc aatagaattt aaagaagtag
    54841 aaaaaagttt ccaagatggc gaccaagtag ttaaagcatt gaaagaaaca accatgagta
    54901 tcgataaagg ggaatttgtt gcaatcatcg gtccttctgg ttcaggtaaa agtacttttt
    54961 tgactttggc aggcggattg cagacgccta gcagtggctc tgtatttatc aatgatcaag
    55021 aattcagtaa tcaaaaagaa agcgaacgcg ttaaactacg ttttaaagaa attggcttta
    55081 ttttgcaagc ttccaatctg gttccttttc tgacagtaaa aaagcagtta ttattagtag
    55141 ataagattaa acatgaacat cgtcaaaaag aagcaaacga attatttgag cagcttggcg
    55201 tggataagtt gatcaacaag tatccagaag aactatctgg tggagaacgg caaagggtcg
    55261 caatcgctcg tgcattgtac aatgatccgt ccattattct agcagatgaa ccaactgcta
    55321 gtttggattc agaaaaggca cgagaagtag tcgatatctt ggcaaaagaa tcaaaagaca
    55381 aaaataaagc aacgatcatg gttactcacg atacacggct gattgacaaa tgcgatagaa
    55441 tatttatgat ggaagatggt gtattgaaag aaaaagaata accaaaaagc aggtggaaaa
    55501 atggtccatc gatacaacaa agatacacag caaaaaattt tgaaagaagc gactgacttg
    55561 tttatgtcca agggatatct cggcacttca acaagagaaa tcgcacaaaa agttgggatc
    55621 acccagccca atctctatca ttatttcggt gataaagaaa aactatatac ggcagtttta
    55681 gaaaaccact taaaagatgt tggaaaagct ttgagagaaa tcgttcaaac aagcgaaacg
    55741 ggatttcaag taaccttgac aaaaatggcg cagtttttga ttgagacaca tctggtcgat
    55801 ttgtttctga tgcttcacga tcttgaaagc aatctttcaa aggaaacaag agatcatctt
    55861 ttctttctat ggaagaaaaa ttatcgtgaa ccttttgaag aaatcttttc agcaaaccaa
    55921 tctgtgctaa gaaatgggat ttcaaaagaa atcgcagcaa ggcatttttt cttagtgtta
    55981 gcaccttaca tcactcggtc gaaaagacct gtggaagaac cgctaaatgt ggcgcaattg
    56041 atcgatttat atttgaatgg tgtattagga gaagcgtaaa gctgttatat agggagaaaa
    56101 tagttaggtg aagaagcaag atatgacgaa gatcgttata tcttgcttct ttatataggt
    56161 agtttttcat aaatacaaat ttgttattct ctcatgatta tcagggtata tttttattta
    56221 ttatgtcggt gttccaggaa gtgttttaat ttaataacga agaaattaat tgtttgtaga
    56281 atgaaggaaa ctatttattg ggtagatgaa tcaccacgaa tagtcaaaag gagatttcag
    56341 gatatgccaa aaatgaaaaa aatcatttta gcagtaatgt tattttctac ggttatatct
    56401 tttacagccc cttttttgct tacttaagct gattttaagt tttggccctt agtctggatc
    56461 tacgaggtga gagtcaagaa tgaaaaaaat aattggagct tttgtatttg caatcattat
    56521 cattattatc tgttttcaaa tagatgtttt tggtcaaaaa aagatagttg gacaaacggt
    56581 cagcagtacg aacagtacgg ttacaaagac aaaggaatca tttgatgata cagaacaaga
    56641 gacattaatc aaaatcggaa aaaatgaccg tacactatat acagatgact cgctaactac
    56701 cccacttgca actataaacg gcggagagct ggcggagttt ctttctgaaa caaaaaatgc
    56761 ttatcagatc aagacaaatg atggctatac tggatattta gccctcgaag atggaaccaa
    56821 agtcacaaag aatatacaaa caaaaccgga agagctatct gatgcagtga ttgtattaga
    56881 tcctgggcat ggcggaaatg atactggggc attaagcaat gatgaacaaa cagaagaaaa
    56941 agatataaca ttaagtacag ccataaaagt aaaaaaggcg ctagaggatg ctggagcaac
    57001 ggtttatttg acccatacta cagatgaatt agtacagctt ggagatatat gcgactacag
    57061 tgaagaaaaa aaggcagatg tctttatcag tttacacgca gactctacgg aatatgcgaa
    57121 tgaagcaact ggaatcacga catattatta ctatggacaa gaggaaacat tagcacagac
    57181 aattgctgac tccttcactg atctgcctct ggattcaaga ggaatctcca ctgggaacta
    57241 tcaagttctt cgagaaaatc ttcaaccttc tattttaatc gagatgggtt atatgaataa
    57301 tgatagtgat ttaaaggaat tagtaacaga cagttatcag cagcagattg ctgcaagttt
    57361 gacacaagca ttgaccactt attttcagta ataaataagc ccagccaaac aaaaatagct
    57421 cctcatattt ttcgtttggc cgggcttatt atcgcagatg caagctttta aacaccgttt
    57481 aaggaagagg tcgtgagcct gccgttcaac tctgagtcac aaggagcaat ttcaaaaaac
    57541 agcttctcat gtttgatgac tattgtgact tgtgaccgag gagttggcat gagaacaccg
    57601 tttaagaaaa aggttgtgaa atcaacgttt tgttctgagt attagagaaa caaacggaaa
    57661 aatagttcct catattttgc cgtttgttga gctaataccg aggatcagat gattgaacac
    57721 cgtttattcg gaacaaggga ctgtgacatg acttttgtca cagttccatt atttttttag
    57781 tagcttcaaa gctttttctg tattggcaaa atgaagtttg gcatattttt caagcatagg
    57841 catgccgcct ttctccaata gttttgccgc tacttggtca gatttcgttc cagctcctga
    57901 cggaagggtg attccagctt cagctgatgc tttgtctaac tcttccaaaa agctggcacg
    57961 actgatgata gaagcagcag caacagctaa atgatattgt tctcctttag taacgaagta
    58021 cagtttttct tgtaattgat ttttttctaa acgcgcatat tttttaaaat ttgcttccgg
    58081 cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc tcgatttttt ttagcagcaa
    58141 atggatcgct tgattgtgca aagcgacttt catccgaacg gcattgtatt ttggttggat
    58201 ctcattgtat ttttctggag ataagacaag cagtttatag gggatggttt tttttaagat
    58261 gtcagcaagc tggatgattt ggctatcttt taactctttt gaatctctaa caccaagttt
    58321 acgtaattct gcaagctgat cacgtttcac ataagcagca catactgtta gcggaccgaa
    58381 atagctgcca tttccgacct catcactacc taaaacggat aattgactga tattttttgg
    58441 caaagaagag gctgtttttg ctggcttggc ttgtttatcc tgtgcctccc cccatttatc
    58501 agcttctatt gctgcattcg taccttgaaa catcactttt ccggatgtat aagcggtaat
    58561 ggtcgtcccg tctttttttg cagcaaagct agtataagga atctgtttat ttaacaaata
    58621 agagcgatag tatgctttca tttgactaag ttggtcgttt gttgctttga taatgacgtt
    58681 gctcataaaa ttctcctcgt ttttatacat aaaactatac cgcaactcac gacttttcgt
    58741 tttctaagag attgcgagac atagcatttt tttaaagttt atcatataat agttaagtat
    58801 gtgaaaaata tttgtggaga acggtgaaag aaccaacttc atggtacact aaataaaact
    58861 ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa aaacacgcta caaagcggtg
    58921 attgccaacc aaacatatac tattatcgga cgggaaacca agcatcatat ggatatcgta
    58981 acgaagctga tcaatgaaca gttagcagaa ttgaaacaat tatcaccaca aatggataat
    59041 gaacaagcag caatcttgat ggctgtcaat gcgctttcgg atcaattgaa gaaacaagaa
    59101 agaatattgg agctggaaga agaaacagca gaattaaaga aaaaaatgat caaatttaca
    59161 gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga acgaagctcg ggaagtattg
    59221 aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg tggaagcaca acagatttta
    59281 aacgagaaac gaaaaggaca aattaaacaa aaatcttcta attaagatcc ttttatcaaa
    59341 ataagaaaaa tttaaaaaag ttagaagaga aaggaacgta gcatgctgag tttgctgatt
    59401 ttatttttat tgctaattgc gtttttttct ggagcacgtc gcgggtttgc tttgcaaatc
    59461 atattcgcga ttggatatgt attgtcattt attgctgccc agcattttta caaaccatta
    59521 gcaagtcatt tggaattata cattccatac ccagctgtca ccccaacatc aaagttagca
    59581 ttttttgatc aggtatttgc ctttcaccta gatgaagctt tttatgcagg aactgctttt
    59641 ctgattattt tgctaattgg ctggctgctt acccgatttg tgggggtttt tgtccatgga
    59701 ttgacgtatg tgccgatctt gagacaggta gactggattg caggaggcat cctgtcacta
    59761 atcatggctt acgttactat atttttgatt ttgcagttac ttgcctttgt tccattagat
    59821 gtggtccaaa atcaattcgg cgagcatagt ttcgctcgtt ttatcgttga gagaacgcca
    59881 tttcttacca acaagatgta tgagctttgg gtaacgaata ttttgaatta aggaaaatag
    59941 tgctggaaaa tatttcgttt tttgtacaga gtagtttatt taagaaggct gcgtagcgta
    60001 tgtccggcac agctcttttt tcatagtgaa agataaggtg ataaaatgaa taaccgaatc
    60061 cttgaaacat tggaatttga aaaagtcaaa cagatggtca gacaatttgt agtgactgca
    60121 caaggaaaag aagaactggc agaattggta ccagtgagtg agaaacaaac gatcacaaac
    60181 tggcttcaag aaactgagga tggattgaaa gtgcaacgtc tgcgaggcgg gatccctata
    60241 cctaaaattg aaaatatccg tccccatatg aaacggattg agatcggagc agacttgaat
    60301 ggagtggagc ttgcacaagt ttccagagta ttgtctacga caagcgaatt gaatcgtttc
    60361 attgatgatc tttctgacag tgaaatcgaa tttgcacgtt tgtatatgtg ggcagatcag
    60421 ttagtcacga tccctgtttt gagccgccgt ttaaaggaag caatcgatga agacggacgc
    60481 gtgactgatg acgcttctcc tgagttaaag agcataagac agaacattcg gcgaagtgaa
    60541 caagctgttc gtgaacagtt agatgggatc gtccgtggga aaaatgcaaa gtatttaagt
    60601 gatgcgatca tcaccatgcg aaatgaccgc tacgttatcc cagtcaaaca agaataccgc
    60661 ggtgtatttg gcggtgtagt ccacgatcaa agtgcttcag gtcagacttt atttattgaa
    60721 ccaaaacaag tggtggattt gaataatcgt ttgcgtcaat accaaatcgc tgaacgaaat
    60781 gagattcaac gtattttaag tgaactttct gctgaacttg ttccccatcg tcaagaaatc
    60841 atccacaata cttatgtgat tggtaaaatg gatctgatga atgcaaaagc acgatttggt
    60901 aaagaagtaa aagccatcgt gccgggaatc agtgaagaca atcatgtcgt cttgaaacaa
    60961 gcaagacatc cactgatcga tcaggaaaaa gtagtttcta atgacatcac aatcggaaaa
    61021 gactatcaag ccatcgtcat tacaggacct aacacaggtg ggaaaacgat cacgttgaag
    61081 actttaggtc tgttacaact gatggggcaa gcaggattac cgattcctgc aggtgaagaa
    61141 agtcaaatcg gtatttttga ggaagtattt gcggatatag gagacgaaca gtcgattgag
    61201 caaagtctat cgaccttttc ttctcatatg acgaacactg tagatatttt atcaaaagtc
    61261 aatgaaaaaa gtctggtatt atttgacgag ctcggtgcag gaacagatcc gcaagaaggt
    61321 gctgctctag caatcgcaat cttagatgat cttggtaaaa agtctgctta tgtgatggca
    61381 acaacgcatt atcctgaatt gaaagtttat ggatataatc gggcaaatac gattaatgcc
    61441 agcatggagt ttgatgtgga tacattaagt ccaacgtatc gtttgctgat tggcgttcct
    61501 ggacgaagca atgcgtttga aatctctagc cgattaggtc ttgatacaga agttattgat
    61561 gaagcgaagc agttgatgaa tgatgagagc caagatttaa acgaaatgat cacagattta
    61621 gagaatcgcc ggaaaatggc agagaccgaa tatctggaaa tgcgtcattt tgtttctgag
    61681 gcacaagaac ttcacgatga tttgaaagaa gcttacagct atttctttga agaacgtgaa
    61741 aaagaaatgg aaaaagcaaa gaaaaaagca aatgaagtcg tgtcagaggc ggaagaaaaa
    61801 gcagaaaaaa tcattgctga tattcgtaaa atgcaacagc agattgggca aggaaatgtg
    61861 aaagagcatc agttgatcga tgcaaagacc caattagcaa atctacatca agaagaaact
    61921 ttgaagaaaa ataaagtatt gaagaaggcg aaagaacaaa aaacgttgaa accgggagac
    61981 gaagtgttgg ttacgactta cggccaaaga gggacgcttt tacgtaaaaa cgggaatcag
    62041 tggcaagtag aaatcggtat attaaaaatg aatgtgtccg aagacgagct gacgcctgtt
    62101 gctcctcaaa aagagccgac acaacgagtt atccatgctg tacgatctga atctagtagt
    62161 cacgtgccga atcaattgga ccttcgagga aaacgatacg aagaagcgct aagtgaggta
    62221 gatcaatatt tggattctgc cattctagct ggttatcctc aagtgactat tgtccacgga
    62281 aaaggaacag gcgcacttcg caagggaatt actgattatc ttaaaaacca tcggagcgtc
    62341 aaaagctttg agtttgcacc ggcaaaccaa ggcggaaatg gtgcgacaat tgttaaattt
    62401 aagtaaagct tactaaaggt aagcagatgg ttcgctatcc aagtatagaa atgctatact
    62461 agtcatagat tttaaaaatg gaggtctttg atatgtcaca agtaattaca gataagacat
    62521 ttaatgaaga aacagataaa ggacttgtat tgattgattt ttgggcaact tggtgtggtc
    62581 catgtcgtat gcaagcaccg attttggatc agttggaaca agaatatgat gaagaagaat
    62641 tcagaattgc taaaatggat gtggatgaaa atccagaaac tcctcaacaa ttcggtatca
    62701 tgagcattcc aacattaatg ttgaaaaaag atggtcaagt agtagaaaaa gctgttggtg
    62761 ttcattcaaa agaacaacta cgtcaaatga tcgatcaata tttataaaaa cttacagcaa
    62821 aaataagctg agggtgggac tgaatccagt tccgtcctcg gtttttttgc ggttgagtta
    62881 aggttattca aattaatatt ttttgagtat aattacgttg tcaggctgga ttgttttttt
    62941 gacagacatg ctataataac tttaattgaa tatgaagggg agtaccagat gaacgaacga
    63001 atcaaaaaca aattagcttt acttcctgat cagcccggct gttacttgat gaaagataaa
    63061 aatggaacga tcatttatgt cggcaaagcc aaaatattaa aaaatcgtgt ccgctcatat
    63121 tttactggta gccataatac gaaaacggaa cggcttgtta gtgaaatcga agactttgaa
    63181 tacatcgtta cagaatcgaa tatcgaagca ttgctcttag agatcaatct gataaagaaa
    63241 aatgatccta aatacaacat tatgttaaag gatgataaaa cctatccttt ccttaagata
    63301 acgaatgaaa aatatccccg tttggtgatt actcgtaagg tactaaaaga caaagcactg
    63361 tattttggac catatcccga tgtcggtgcg gcgaatgaga caaagaagat cctcgatcga
    63421 ttatttcctc tgagaaaatg caaacctagt cagacgaagg agccttgtct ttactatcat
    63481 ttgggacaat gcctgtgtcc ttattatttc gacgtagatc cgaaagtcta tacgaaaatc
    63541 gttgatgaag tgaaaaactt tttaaacgga ggacacgaaa aaatcgagtc agagatccat
    63601 cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa aagctgcaga ataccgagac
    63661 cagatccgtg ccattgagac aatcatgact cgccaaaaaa tgacaaatac tgatttactg
    63721 gatcgagatg tatttggtta tgcgattgat aaaggatgga tgtgtgtcca agtgtttttc
    63781 gtaagacaag gaaagctaat tgaacgagat gtttcaatgt ttcctttcta taacgaagca
    63841 gaagaagatt tcttgactta cctcggtcag ttttatcagg aaaatgagca tttcattcct
    63901 aaagaagtct tgattcctga tacgatcgac aagcccagtg tagaagcttt gttagctacg
    63961 aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag ttgaattggc gagtaagaat
    64021 gcctcagttg ctttgaatga acggtttgat ctgattgcca gaaaacagga acggacgatc
    64081 ggagcagttg aacgtttagg aaacgcgatg aatatcccga ccccaatacg tatcgagtct
    64141 tttgataact ctaatatcat ggggacgaat cctgtggctg cgatggtcgt attcattgat
    64201 ggaaaaccgg caagaaaaga atatcggaaa tataaaataa aaacagtcaa cggaccggat
    64261 gattatgcat caatgagaga agtcatctat cgcagatatt cgcgagtgat tcgcgaaggg
    64321 ttgcctttac ctgatttgat cttgatcgat ggaggaaaag gacaagtaga tgtggcaaaa
    64381 gatgtcttag aaaatcaatt aggaatcgat attcctattg ctggcatggt aaaaaacgat
    64441 aaacataaaa caaatgagtt gctattcggt acagatttgg ctgtagtacc gcttgagcga
    64501 aattcaccag agtttttctt actccagcga atccaagatg aagtccatcg attcgcaatc
    64561 actttccatc ggcagctacg cagtaaaaac agttttgctt cccgattaga tgaaattgaa
    64621 gggttaggac caaaacgaaa aaaaatgctg cttaaagagt tcaaatcgtt gaaaaatatc
    64681 acagcagcca cgacagaaga attacaagaa atcggactgc cgaaaaatgt cgcacaaaac
    64741 gtatttgata aattacatca ggaatgagga aagggcactt tacaaagaaa gtaaagtgtc
    64801 ctatcatttt atgtacaaca aaaagagcgt ttacgttttg ttggtaaata ataaatcgct
    64861 tacgtctggt gtctaaggat atatgtgatt tcttgaagaa ggagtgaaga aatgttagaa
    64921 gtcaaggatc ttgttaaaac ttttgggaac tacacagctg tcgatcatgt ctctttccaa
    64981 atccccgatg gaaaaatcat ggggctaatt ggccaaaacg gggctgggaa gacaacgact
    65041 tttcgtttga tcttggattt tttgacacaa gaccaaggag aagtgttatg gaatggccat
    65101 catcttgggg agaaagatta tgatgtcatc ggttatcttc cggaggaacg gggactttat
    65161 ccgaaagtaa cgattcaaga ccaactgctt tattttgcag aacttagagg gaaatcaaga
    65221 aaagagattg aaccaaaaat cgacttttgg atggaaaaat ttcaggtaaa agggaaaaag
    65281 acggataaag taaaatcttt gtctaaagga aatcagcaaa aagtccagct gatcgccacg
    65341 ttaatccatg aacctaaatt gatcattttg gatgagccat tcagcgggtt agatccggta
    65401 aatgctgaat tgctaaaaga tggaatattg gaattgaaaa aacatggttc atgtgtcatt
    65461 ttttctagcc acaatatgga taatgtagaa aaaatctgcg atcatttgat catgttgcga
    65521 aacggaaaaa tggttttgaa tggaaaagta catgagattc gtgaatcatt tggccgtacg
    65581 aagttattct tggaatctgg tttaagccaa caggaagtag aagaaatcga aggcgtgaaa
    65641 aaagtgatcc ttcatgaaga caatacgtta gagattacct tagaagatcc tgaagctggg
    65701 aaagaaatct ttactcgtgc gactcagttc ggctacattc ctatgtttaa ccagcaaccg
    65761 ccaactttgg aagagatttt caaattgaaa gcaggtgaaa tcgatgcgta agttttggat
    65821 tattaccaaa gatgtctatt taaaaaatgt taagtcgatt tctttcttga tcatgatatt
    65881 agtccctttt atcatgctgg gtatcattta tgtagcagga aactttgctc aacaaaatag
    65941 tgatgtcaat aaaatcggcg tattggctga taatgagcaa atcagtcagc aaatgagcca
    66001 agtcaaaaca gatgattttg agttcaaagc agttgattct gaagaaaaag cacaaaaaga
    66061 gctttcggaa gaaaaaatcg atgcgtattt agtagtctca actgagaatg ataagataaa
    66121 tgggatgctt tatagcgaaa attcattagg acaaagtaca caactactga tacaacaaca
    66181 actgactggc ttgcaatcga tgcttcgagc gagtcaatta ggcatttcac cagaagaagt
    66241 agctagcttg agccaacaag caagtttgac tcgacaaaaa gtcaattttg attcaaaagg
    66301 aaaaatgaca gtcggagaag ataactcgac agtccaatac gtcgtaagtt atgtagcaac
    66361 gattgtcttg ttcattatta ttttgacgta tgcgcagatc attgcacaag agatcgcttc
    66421 tgaaaaaggc acgcggatca tggaagtgat cctgtccagt acgactgcac aaaagcattt
    66481 ttacggaaaa ttaacaggtg ttttacttgt tgcattaacg caaatggcat tatacggtat
    66541 tatttttggt ataggataca gtcaattcaa agatatggaa atggtcaaaa atatattaga
    66601 cggtatctca ttggataact tgattggacc attcttgtgg tattctcttc tattcatgat
    66661 ctttggcatt ttgatttttt ctgttttagc agctttatgt ggctctttgg tcaacaaagc
    66721 agaagataca gcaaaagcaa ttttgccagt gacttatcta tctctaggcg gatacatgct
    66781 tggattgata ctaggagctt cagatccaaa caatatcgtt attcggatca cttcgtatat
    66841 tccattctta tcttcatata ttatgccgat tcgcttagca aatgagacag tcggtatggg
    66901 tggagcattg atttccttgt tggtacttgt agccgtaaca ttcttattga tgatcttgtc
    66961 tgcaaacatg tacaaatcga atgtactcgt ttatagcgaa ggcggattat ggagttcatt
    67021 gaaacaatcg atttcaatta tgaggaatga acgaaaaaaa gcataaaaca atcctaaaat
    67081 acagaaggaa gctggacatc tattgttccc agcttctttt tgtatagacc tattttattt
    67141 attttactat attggatata tattcattga aaatagtgta taacaacttt ataatattga
    67201 gtataccaat ttttggaggc gttttaatgg accgttattt agatttactt tcagaaaaat
    67261 tcccctcaac ggaggatgtc atcacggaaa tcatcaatct cgaagcaatt ttgcagcttc
    67321 caaaaggaac agagttattt ttgagtgata tacatggcga atttcctgct tttgatcata
    67381 ttctgcgtat cggttcagga aatcttaaag aaaagatcaa agaccttttt tctgaacaaa
    67441 tgacggaaac ggatatcagc caattcacta tttttacggc ttatccagaa tatgcattaa
    67501 cgactgattg gtacaaagaa caagataaat cgcagctgat ccatcatttg atcgatcttt
    67561 tacggtttac gacagtcaaa tatacccggt caaaagttcg aaaaggattg ccgaaagaat
    67621 atagctatat cattgaggaa ctcttgtata tcgatgaccg tatcaatgga aaaaaagact
    67681 atgtaaaaaa aatcattgaa caacttgttt tgatgaagga agaagaaaaa tttttaacga
    67741 aacttgcaca gacgatccaa aaatctgtca tcgatcattt gcatatcgtt ggagatattt
    67801 ttgaccgagg aactcaagca gccaaagtga tggatcgaat catggatttt tcttctgtag
    67861 atatccaatg gggaaatcat gatattttgt ggattggtgc gtattgcggc tctgaggcat
    67921 gtttactgac acttttacgg attgcagcac gctataatta tttatttgaa ttagagaaag
    67981 aatatgggtt gaatttacgt ccattgtttt cttttgctca tcaaacttat cagaagaatc
    68041 cagtatttgc gcctaaaaac agtaaaaatc tatccgaaag agagcaagaa gaattagaaa
    68101 aaatccatca ggcactttct atccttcaat tcaaatcaga acatcagtta ttggatcgtc
    68161 gacctgaatt taaaatggac gatcgtaaat tgctggaaaa aatcgactat gaagcatcaa
    68221 cgattgattt agaaggtcag ctctatgagt tgaaaggaac ttgttttcaa acgatccagc
    68281 cagatgatcc aaaaaaattc ttggaagagg aacaaaaagt gattgatagt ttgatgtatt
    68341 cattccagca ctctttgaga atgcaaaaac atatgacgtt tttgatcgaa aaagggggaa
    68401 tgtatctcac gaacaatcag catgtgctgt ttcatggctg tatacctgta gacgagaagg
    68461 gacagctctt agcatttgaa ttagaccaat cttatcgcgg aaaagaactg ctgggctttt
    68521 ttgaaaaaca gataactgaa agcgcaaaag atctgactgc aaaagaagat ttagcaaccg
    68581 atttgatttg gtatgcgtgg agtgggccat tttcgccttt attcggcaag agtaaaatgg
    68641 cgacttttga acgttatttt ctcacagagc ctcatacgca cgtcgagaaa agcaatcctt
    68701 attatcactt gcgagatgaa gagtggttca gtgaaaaaat tttagcagaa ttcgacacaa
    68761 aagaagaagg gtcagcaatc attaatgggc acacgccagt aaaagtaaaa aaaggggagt
    68821 caccgatcaa agcaaatgga aaagtatttg tcattgacgg aggtttatcc aaagcttatc
    68881 aaaaaacgac aggaattgcc ggttattcac tattatgcaa ttcttatggt ttccaaatcg
    68941 tgacacatta tccgtttttg cctattgaac aacaatttgc aaaaaaatcc gaccaaacaa
    69001 atgtgaaaaa agtaatcgaa cagcaactac cacgcaaact caatcgtgat acaacaaaag
    69061 gtatggaact gcagcagcag attacccagc tcaaaaggct gttggactat cgaaaggacg
    69121 attgagaaga attccagtga acattgttcc atagactgga gactgagaca aaagtcgtgt
    69181 cgcagtctct ttttccggat aaacggtgtt cttctgccaa ctcctcggtc acaagtcaca
    69241 ctattcaaca aacatgaaaa gctgtttttt gaaattgctc cttgtgactc agagttgaac
    69301 ggcaggctca cgacctcttg gtataaacgg tgttcaaaag ccagtttctc ggtattaagc
    69361 caaatttatg caaaaatgtg aaaagccatt ttcacaaaat ttttcttaat actcgaaact
    69421 aaacagcttt ttcacaaccc cctcagaaag tctattaaaa ttcattcaca aagaaatcga
    69481 ataagcgctg ttcagatgga tgtacacggt gggtaagttc cggatgccat tgaacaccga
    69541 ggattttatg catatcatct ttgctttgaa ccgcttcaac gagtccatcg ttagaaaaag
    69601 cgatggcttt taataaaggt gaaagatctt tgactgcctg atgatggtaa gaattcacaa
    69661 aatatggatc ggggagaagg ttgcttaaca aactgtctga aacaatcttg acttcgtgag
    69721 tcgtaaattg cggggcagtg ggctgttgtt catgttttac tgaccacttt ggatataatg
    69781 aaagatcttg ataaagtgta ccttccaaaa cgacattcaa tagttggagt ccccggcaga
    69841 ctgcaaaaat tggttttctt tgtttcaatg cttccaagat cagtgcctgt tcaaattggt
    69901 ctcgttgaat atttgtttcc gttagctttg gatgaggatc ttccatgtaa agttgtggac
    69961 tgacatcttg accacctgca agaataagtt tgtcgatttg tccaatcagt tgaggagctg
    70021 aatctggaga catgactggc agaataagag gcaatccttg agcatctaaa actgcatcca
    70081 cgaatccttg cggtgtatag ctcacttgat ttccttggaa agtatctgtt gctcgaatca
    70141 gttgatttcc ggcaatgccg atgattggtt tcatttgtat tccccctaaa gtagacttat
    70201 tgtaacaagg atttaaaaaa aattaaacta tgtaagactt gcccctatac ttatctgttc
    70261 ttgctgtgtt actatctgta aagagaaatt tcgtgtgaaa ttcatgacga tagttgaaaa
    70321 tattgcttaa tttgttataa taaaaagtaa gcagaaataa aaaggttggg cgtaagcttc
    70381 aaggatctag ttcttcaagg ggtgagaagg acattttatt gaaggggcct gcctatacta
    70441 agttatcttt agaggtacat actttcaatg aaaaaagaaa gcatgtacct cttttttaaa
    70501 tcgaaaggaa gtgaagcaag tgttagaagc tttggaacaa atccgaaaag cagaagaaga
    70561 aaatgaaaag cgtgctcaaa cactgcggca gcagctgaag atctatgcaa cagaaaaaga
    70621 gcaggaattg gaaacgatcc aaacaaacta tcgacaacaa ctaaaacagt tggtggaaga
    70681 aaaagaacaa tttgaaatac aagaattaga aaaagaaaaa atgaaattga aagcagaagc
    70741 agacaaggtg caagcaactt tgaaagatca atatcaaaag catcagagtc aagcgatcgg
    70801 tgcgatcatt gaaagggtga aagaaactta tggccgtcac taagatggaa aaagtgaccc
    70861 tcatttcaga taaaagaaat caggagagga ttttacaagc agtacaagga atccaaaacg
    70921 ttgaaatccg ggatctgtat caaaatagaa caaacaacca gtgggtggag aagtattttc
    70981 cagataccac gattattgat aaagaagcaa aattaagcgc tttaggtaat cggttgaaag
    71041 acatccaaga agctatccaa ttcatacaac accacggcga tagccgtcaa aagaaactcc
    71101 atttgaaacg tcaagaactt tccttacaag atttggaagc caattattcc gaagaaaatt
    71161 tcataaataa attgaaagaa gtccttgatt taaaagaaaa gtgggaacaa ctaactgaag
    71221 aacgtgatcg tttagaagaa gaggaagaat ggcttcttca ttggcaacag ctggatattg
    71281 ctccaagtat gtatgcaagt aagacgacca tttttgagat ggggactgtg agtgcagcaa
    71341 attttgaaag tttcaaggaa gaactgagta agcttgatga cgtttatatg gaagaaatag
    71401 attatacgcc aaaacaagtt tatctttcgt atgttgcttt aaaaaaatca gttgctgtta
    71461 tagaagaaat tgctgggaaa tacgggttca ataaagaaac gtatccttat gaacaaaatc
    71521 ctaaagaaaa actgcaagag accaagcagc aattatccat tgttcacgag cagcaaaaga
    71581 agataacgac agcgttaggt cagtgcagtg gctatatcca agaatttgaa tgggtagaag
    71641 aagtcacact tgccgttgca gaacgagaaa aaataaaaga acggtttatc catgcgactt
    71701 atctgatcgt tttgcaaggt tgggtagatg cagaagaaaa acagaatttg ctgcatgtgt
    71761 taaacgaaac cgtatcagaa gaagatatat acgtcagttt cgaaacacca aactcagaag
    71821 agattcagca ggaagtccct actaaattga aaaaccatcc gcttgtcgaa ccttttgaat
    71881 tgctgactga aatgtacagt ctgccaaaat atgaagaagt agatccaaca ccttggatga
    71941 caccatttta tcttgtcttc ttcgggatga tggtcgcaga tgttggttat ggtttgctga
    72001 tgctcattgg gacaatacta gctcaaaaac tacttgttct accacgcggg atgaaacgtt
    72061 tcgtaaaatt ctttgagatt ctctcgatac catcgatcat ctggggattg atctacagtt
    72121 catttttcgg acagtcatta ccaaaagaac tgtttggcat ccgcttgcct ttcccaatcc
    72181 tatctacgac ggatgacgtc aatacgattt tgattttatc cgtgattttc ggtctgatac
    72241 agattttggt tggattattt gtggcagcaa aggaaaatat caggagaaaa gattacttga
    72301 gtgctgtcag tgatggattt gcctggcaag ggatcctaat tgggatcgtt attgcgttgg
    72361 ttggtgccat gttgataaaa aatacagcat ttgtttacct tggcgctggg ctatcgatca
    72421 ttgctgcctt atgtatcttg atcgttccga ttatccaaac gccgtctaaa gtcaaaggtg
    72481 ctgccaaagg tgcgtataat ctttacggac tgaccggcta tatcggtgat ttagtcagtt
    72541 atacacgttt gatggcattg ggtatttcag gcggaagtat tggagcggca ttcaatatgc
    72601 tagttgcttt catgccgcca gctgctcgat tcagtgtagg gatccttttg atcatcgctt
    72661 tacacgcact gaatatgttt ttgacactgc taagtgccta tgtccatggc gcacgattgc
    72721 agtatgtcga attttttggg aagttttata caggaggcgg acgtgccttt gatccgctga
    72781 aaacggcgga aaaatacgtc aacatcaatc acaagaaaaa aaacaataaa taattggagg
    72841 atttttatat aatgatggat tacttaatta gtcaaaatgg tggaatggta tttgcggtat
    72901 tagcgatggc aacagcgacg attttttcag ggatcggttc agctaaaggt gttggaatga
    72961 caggtgaggc agcagcagca ttgacaacaa gtcaacccga aaaattcggg caggctctga
    73021 ttctgcaatt gcttccaggc acgcaaggtt tgtatggatt cgttattgcc tttttgattt
    73081 ttatcaactt aggaaatgac atgtcagttg tacaaggact gaactttcta ggtgcttctc
    73141 tgccgattgc ttttacaggc ttgttttcag gtattgccca aggaaaagtc gcagcggcag
    73201 gtattcaaat tttagcgaaa aaacctgaac atgctacaaa aggaatcatt tttgctgcga
    73261 tggttgaaac atacgcgatt ttaggtttcg ttatttcctt cctattagtg ttgaatgcct
    73321 aagcataaag gaggaaaagc agatgaacgc cattgaaaaa atcatctctc agatgaacga
    73381 agcagcagag caggaaagag cggcgttaga acaagaagag cgaatgaaga tcgatcaaaa
    73441 ctttgaacaa aaacgtacgc aagttgaaac agaacatcaa aaacaaaaag aaaaacaaat
    73501 cgaattactc gaaaaaaaat accgtcagct aaggaatcgc cagcaagtag aagtacgaca
    73561 agaaaatttg aatgcgaagc aggaattttt aagacgattg tttgcagatg cggtaactga
    73621 aatggaaaat tgggatgaat ctgaacaaat ccagtttatc aaaaatgcat tgtattcatt
    73681 acctttgaca ggaaaagtag cattcattgc tggagaaaaa tcagcagcct atctttctca
    73741 gacgttgttg gatgaatgga acaatgaact tccctttatg atggttttaa gtgatgaaac
    73801 tgttgctgat caagcgggtt ttctgataaa cgatcaagga gttcagtata attttctttt
    73861 cagcagcctt gtacaagata tccaaggaac gatgagtttt gagattgcca atcaattatt
    73921 cgaataagga gggatcagtg tatggaatac cacgaactaa atcccttgat tcgcggaaga
    73981 gagcttgaac ttttgtccaa tgagacattt gaacggatga ttcaaacaaa ttctgtcgaa
    74041 gcacttggtg aaatattgaa atccacgatt tacagtccat atatttatga cggatatgaa
    74101 tatgacttcg aacagaatct tttaaaggag cagagccgtc tatttcgctg gttaaaggaa
    74161 tcagcaccgg agcctgaaat cgtttggatc tatacgatgc gttatacttt ccataatctg
    74221 aaggttttga caaaagctga gatgacaaat caaaatctgg accatttgta tatcgacgat
    74281 ggattttatt cattagaaac cttgaaggat gccattcata cgcaagtttc aaatgaactt
    74341 cctgaaagca ttatggacag tatacgagaa gtacgtgatt attttgaaga gtctaccatt
    74401 cttcagggaa ttgacgtgat ttatgatcga cagtttttga cagagcagcg acggcttgcc
    74461 gataagctag gatatcaaga attgctgaca gaagtcattg cttttattga cttgaccaat
    74521 atcacaacga tggctcgagg aatattgcag aaaagaacat atgggttcat gacgacggta
    74581 ctttccagct cgggaagtat cgaaaaagaa gtatttttga actttgtaga taaagatatg
    74641 gaagattaca cgaattttct attagagaca gattatgctg attttctgcg ccctgtcatc
    74701 cgtgagggaa atattgatct cgtaaagctt gaacagttga aagatgatta tctaagttca
    74761 ttgtatcaag aagcacaaac ccaggctttc ggaccactgc cactactagc ctttttgaac
    74821 gcaaaagagg tagaaagcaa aaaccttcgt ttactggtta tcggcaaaag aagccatttc
    74881 gaatcagaaa caatcagaaa gcgggtgaga caagtctatg acgcataaga tcggtgtagt
    74941 tggcgacaaa gattctgttt tgccattcaa attgtttggc tttgatgttc gttatggtac
    75001 aaccaagcaa accgttcgtc aggcaattga agaaatggca aagaatgagt atggcgtgat
    75061 ttatgtcaca gagcaatgtg cagcattgag cccagaaacg atcgaacgtt acaaggaaca
    75121 aatgatacct gctatcgtat tgattcccaa tcatcaagga tcattaggga tagggctgga
    75181 agaaattcaa aaaaacgtag aaaaagcagt aggtcaaaat atcttatagt atgaaaagga
    75241 gatagaaatt ttgcaaattg gaaaaatcat aaaagtctcc ggacctcttg taatggctga
    75301 gaacatgtct gatgccagta tccaagacat gtgtctagtg ggagatctag gagttatcgg
    75361 agaaattatc gagatgcgtg gcgatgtggc gtctattcaa gtatatgaag agacttctgg
    75421 aattggtcca ggcgaaccgg ttcgctctac tggtgaagct ttatctgttg aattaggacc
    75481 agggatcatt tcacaaatgt ttgacggaat ccaacggcca ttagatacct ttatggaaat
    75541 cacacaaagt aatttcttag ggcgcggagt tcaattgccc gcattggacc atgagaaaaa
    75601 atggtggttc gaaccaacag tagaagccgg agaaacggtg agtgctggag atgttatcgg
    75661 gatcgtagaa gaaacaaaag ttataaaaca taaaatcatg gtgccaaatg ggataaaagg
    75721 aacgatcaaa acaatcgaaa aaggttcatt cactattgat gaaacagtat gtgtcattga
    75781 aaccgaaaat ggtgacaaaa atttgacgat gcttcaaaaa tggccagtaa gacgtagtcg
    75841 gccaatcaaa gaaaaattaa acccggatgc acccatgatc actggacagc gagtgattga
    75901 tacattcttc ccagtgacaa aaggaggagc agcagctgta cctggtcctt ttggtgcagg
    75961 gaaaacagtc gttcagcatc aaattgcaaa atgggccgat gtggacatgg ttgtctatgt
    76021 gggatgtggc gagcgtggaa atgagatgac ggatgtttta aatgaattcc ctgaattgat
    76081 tgaccctaat acaggagaat cgttgatgga acgtacggta ttgattgcta atacatcaaa
    76141 catgccagta gcagctcgag aagcttctat ctatacggga atcacaattg cggaatattt
    76201 ccgtgacatg ggttatgacg ttgctatcat ggcagactcc acttcacgat gggcagaagc
    76261 acttcgtgag atgagcggac gtttggaaga aatgcctggt gatgaaggtt atccagctta
    76321 tcttggttca cggcttgcgg aatactatga acgatctgga agagtcattg cattaggatc
    76381 agaaggtcgc gaaggaagta taaccgcaat cagtgctgta tccccgtctg gaggggatat
    76441 ttcggaacca gtcacacaaa atacattgcg ggtcgtaaaa gtattctggg gtcttgattc
    76501 cagtttagcc caaaaaagac attttccttc gatcgattgg atacaaagct attctcttta
    76561 ttcaacagaa gtagggaagt atatggatca tatccttcaa gctgactggg catccatggt
    76621 caccgagggc atgcgtattt tgcaggaaga acagcagctg aatgaaatcg tgcgtttggt
    76681 agggattgat tccctttcag ataatgaccg tctgacatta gaagtggcaa aatccattcg
    76741 tgaagattat ttgcagcaaa atgcttttga tgatgtcgat acattcactt caagagagaa
    76801 acaattcaaa atgttgaaga tgatcttgac ctttggtgaa gaaggacgcc gtgcgttgtc
    76861 gttaggtgct tatttaagtg agatcatgga cggtaccgta tcgattcgtg aaaggatcgg
    76921 acgaagcaaa tatatcccgg aaacagaatt agaaaaacta gatcaaatta ttggtgaaat
    76981 caagcaaacg atgcaaacga tcgtttcaga aggaggcatg agcaatgatt aaagaatatc
    77041 gtacgatcgg tgaagtcgtc ggtccattga tggcggttga aaaagttgct ggtgtcaaat
    77101 atgaagaatt aatcgaagtc cgtatgcaaa atggcgagat tcgtcgtgga caagtattgg
    77161 aagtccaaga agataaagca atggtccaaa tattcgaagg aacaagtggt atcaatttac
    77221 gtgactcttc tgtacgattt ttaggccatc ctttagaact aggtgtatcg gaagacatga
    77281 tcggtcgagt attcgatggt ttgggacgac cgaaagataa cggaccagag atcctgcctg
    77341 aaaagcattt ggatattaac ggagaagtaa tcaatccagt agctcgggat tatccagatg
    77401 agtttatcca aacaggtatc tctgcaatcg atcatttgaa tacattagtt cgtgggcaaa
    77461 aattaccagt attttctggc tctggtcttc ctcataaaga attagctgct cagatcgcgc
    77521 gacaagcaac ggtactcgat tcatcagatg atttcgccgt agtctttgct gctattggaa
    77581 tcacttttga agaagctgaa tttttcatgg aagattttcg tcaaacgggc gcaatcgacc
    77641 gttctgtcat gtttatgaat ttagcaaacg accctgcaat cgaacgtatc gctactcctc
    77701 gtatggcttt gactgctgca gaatatcttg cttatgaaaa gggaatgcat gttttggtta
    77761 tcatgacaga catgaccaac tatgcagagg cattgcgaga gatttctgca gcacggcgag
    77821 aagttccagg aagaagagga tatcctgggt atctttacac gaacttagct actttattcg
    77881 aacgagcagg aagaatccgc ggactaaaag gatcggttac tcaaattccg attctaacaa
    77941 tgccagaaga cgataaaact catccaatcc ctgacttgac tggttatatt acagaaggac
    78001 agatcatctt gactagagag ctttacaaga gtgggattca gccaccaatt gatgtactcc
    78061 cgtctctttc gcggttaaaa gataagggaa ctggacccgg aaagacaaga gaagaccatg
    78121 cagctacgat gaatcagctt tttgcagcct atgcacaagg aaaacaggca aaagaattag
    78181 ccgtagtttt aggtgaatct gctttatctg atgtagacaa gatctatgcg aagtttgctg
    78241 agcggtttga aaaagaatat gtcaatcaag gattctacac gaaccgttcg attactgaaa
    78301 cacttgatct agggtgggaa ctgctatcga tgttgccaag aaccgaatta aaacgaatca
    78361 aagacgacat gctggacaaa tatctgccag aagggaagtg agttctgatg gcacgattga
    78421 atgtgaatcc gacccgcatg gaattgactc gattgaaaaa acagttgaca actgccacaa
    78481 gagggcataa attattaaaa gataagcaag atgaactgat gcgtcaattc atcctgttga
    78541 ttcgaaagaa caaccagctg cgtcaagaaa tggaagagtc tatgacagaa gcaatgtctg
    78601 attttgtatt ggcaaatgct tcgatcacgg aacctttcat cgaagaatta tttgttttgc
    78661 cggcggaaaa cgttgatttg tctatcgtag acaaaaatat catgagtgta aaagtacctg
    78721 tgatgaattt tgaatatgat gaacagctga cagacacacc tttggaatac gggtatttga
    78781 attccagtgc cgaactggat cggtcaatcg atcggtttac taccctgctt ccaaaacttt
    78841 tggaactgac agaaatcgaa aaaacttgtc aactgatggc gagtgaaatc gaaaagacac
    78901 gtcggcgagt caatgcatta gaatatatga cgatccctca attagaagaa acgatctatt
    78961 atattcgtat gaagctggaa gaaaacgagc gcgcagaagt aacaaggttg atcaaagtaa
    79021 aaaatatggg taccactgac taagtggtat ctttttttaa aagaggaaaa cgatgattaa
    79081 acaaagcaaa caaataaaga aacgtttgcc ggctgttcag cttctcgcat taggattttt
    79141 tctcctgatt ttagttggcg gcggcctact taccctacca ttttttagta atagtgggca
    79201 tgggacaaat tttatcgatg cgcttttcac tgcaacttct gctgtctgtg taacgggatt
    79261 gacaacattg aatacagcag agcattggaa cagtgctggt caatttttga tcatgctttt
    79321 gattgagatc ggcggtcttg gttttatgat ggttccgatt ctatgctttg cattgataaa
    79381 gaagaaagtc agtctcagca cacgtattat attaaaagaa gcgctgaatc tagaagaagt
    79441 atcaggtgtg atgaagctca tgctttacat cctaaaattg gcctttgtga tccaattgat
    79501 tggtacggtt gctttatctt ttgtatttgt gcctgaattt ggctggttga aaggtatctg
    79561 gtacagtttg ttccatgcaa tctctagttt ttgtaatgca gggtttgatt tgttaggaga
    79621 cagtttagcc aatcaccagc aaaatgttta tttgatcatg gtggtatctg ctttgatcat
    79681 ttctggtggg ttaggcttca tcgtatggcg tgatttactg gaatatcatc agaaaaagaa
    79741 gatgacgatt cattcaaaaa tcgcattaac cgttacaggt acccttttat taggcggatt
    79801 cgttgtgttc tttttcacag agcgcaatgg ggcaacgcta gtggaaggga atatgtttca
    79861 acgattggca aacacctttt ttatgagtgt gacacctaga acggctggat attattcagt
    79921 tgattacctc catatgagcc atgcaggtct gattttaacg atgtttttga tgtacatcgg
    79981 cggaacatct gggtcgacgg ccggcggatt gaaaacaact acgttaggtg tgttgctgat
    80041 ccagatgcgt tcgatgttga aaggacgtac gagagcggaa gcatttggcc gtaccatccg
    80101 acaaggcgca gtgttgcggg cacttacttt attttttgtt acgttgacgt tgtgtgtagt
    80161 cgccatcatg attttatcgg tgactgaaac gatacctaaa acttctggca tcgaatatat
    80221 tgcttttgag gtcttctctg cgtttggtac agtaggtctt acgatgggat tgacgccaga
    80281 cttgactgca atagggaaat tgatcattat agcgcttatg tacattggac gagttggtgt
    80341 attgactgtg gtcttctctc tgatgacaaa agcacaaaga acacaatcaa aatacaaata
    80401 tccagaagaa agtgtcatga ttggataatt tcatcttctg acagaaacct agtgatttag
    80461 taaaataaga aaataaaata atttttcttg cttcttggat actttaagtg caaaatgttc
    80521 gtttattttt tgaggcacta cccgttgttg aacctcgggt agtgcctctt ttttattgaa
    80581 aggaatcaat aagatttgat tcctttattt atcaataggt tataattcat atttttttat
    80641 tttttctttt acgatactta gtccagaagc taaaatttca aggttattag aggataggga
    80701 aattcgaacg aatgcatctg gttgcgcacg tgtagtaaag cgtctggaat gataaagtcg
    80761 gataccgtta gcaagaaagt cattctcaat tatcttaggg tcaatctcag gatcaactgg
    80821 aagaattcga tagtaaggga cacgattcgt tggtttttct actttgaaaa gagaatcaaa
    80881 aagctgattg gctctttttg taagacttaa tttagtttct aagatcctat ttgcttcatt
    80941 tgaatggagg atttgagtga tgacttccac atctaatcca gaagttttca catttatatt
    81001 aaaaattgct tgttctatct tgctttggac atgtactgga actacaagat aagcaactcg
    81061 caatccagaa caaataaatt ttgtcattcc agctaagtag attgtttggt taggtaataa
    81121 attttggaaa ggaggaatga tcgtatgttg atagtaagtt gttaaaaatg aatggatatc
    81181 atcttcaata acccatagac cttctttttc aatcacttcg gctaattgct ctcgtctttt
    81241 gtgagagatt tgaaacccta taggattatt acaagaaggc ataagaaaga caccatgaat
    81301 cttctttttt ctacattcct gaatcaatat atcaggtcgc attccttgct cgtcaaaatc
    81361 aattggaacg atctctaaat gatataactg agccaattca atgaaattag cgtatgtata
    81421 tcggtctgtt gctattctat cacctggaga gaaaagtgca gaaagtgtga tagccaatcc
    81481 attttgaaca ccggaaacaa tagatagaga ttctaaggac ggaatgactc cttgagtttt
    81541 taaccaattt gctgcagtag tcaattgaaa atttgtgcct ttgggatctt ggtaattaag
    81601 caaaccaaca agatttgggt tattactagt tgattgaata aacgggacaa cgaatttatt
    81661 acattcttca aaagaactaa ccagaccaaa atcaatgata tgttcggtta cttcattggt
    81721 agagatggtg atggactcaa agacattggg agaaataaat gtgccacttc caatattcgt
    81781 atacagcaaa cctttctcaa ttccatattt ataagccttg ccaacggtag taaaatttaa
    81841 atctaaataa tcagctagct ctctttggga gggtaactga gtgtttttct gtaatttatt
    81901 ctttaaaata tcttgttcta attgttgaat caacgatcga taataaggac gcttcaattt
    81961 ttgtttgtct ggtcgccaag ttaacgggta attgtcataa gaattaatag gcattttctc
    82021 cactccttaa aattgtatac catacaattt taccattgat tgtattgatt ttagaagtta
    82081 atcttttgtg aggagggaat tgtctatgaa aattattaat tttgaggaag tagtagcttc
    82141 ttttagtttt gaagaagcga ttcaagtaat gaaaaaatgt ttttttgatt atgaaagtgg
    82201 taaaatcagc caaaatcctc gattggtgga aattttacct gacggaaaag gcaaaaatat
    82261 gtttgcgatg atgcctgctt atttggggga gaacagatat tttggcacaa aagtcattac
    82321 cgcttttccg gataatcatc agtatcattt accttctcat ttaggagaga ttttcttgtt
    82381 tgatgctcaa aacggactac ccaaagcatt gataaacgca aatgcagtca cctggattcg
    82441 aacagctgct gtatctgcgc tggcaacaga ttacttagca aatccgcatg cagaaacact
    82501 tgctttaatt ggagctggtc aacaagcatt gtcgcatttg actgcgattt ctaccattcg
    82561 tcccatcact acagtgtttg tttacgatat tttagaagaa agacgaaata cttttatcgc
    82621 taaagctaaa aaacagtatc ctaaaataac gtttataaat gcaatatcag tagaagatgc
    82681 agtacgtcca gcggaaatca tttgtacgtt gacctctagt aaagaggcat ttcttcaaaa
    82741 agagtgggta agggaagacg cacatatcaa tgctgtgggc acatttactc ctgatacaag
    82801 agagataagt acatcgttga tggaaacttc tcaaatctat gtagatgatt actcagctgc
    82861 cttaacagaa agcggggacc tgcttatagc catggcagaa ggagatttag aggaatcttg
    82921 tattgttggt acattaggcg aattagtttc tgagaaaaaa acattttata aaggagaatc
    82981 gaggccgacg attttcgacg cagttggtct agctattgaa gatctttgtt gtgcagaata
    83041 tatttataac aaattagaga aaggggcttt aaaatgaact tagattatta cgtagttgat
    83101 gcatttgcag atgaggtgtt caaaggaaat ccagcagcgg tttatgtgct agaagaatgg
    83161 ctgccagaag gtacgatgca aaaaattgcc attgaaaaca atttatctga aacagcattt
    83221 acagtgaaga aaaaccaaga atttgagctt agatggttta cgccagatcg agaaatcgat
    83281 ctttgtggtc acgcgacatt agcaacagca tttgttttat ttaactatta caaaatacct
    83341 gatgaaacga ttaaattttc tactcaaagc ggtaatctat ttgtgacaag aattaaagat
    83401 tattattaca tggattttcc tagtattatg ccgaaaaaag tgccgatatt gtctgaatat
    83461 gaagaagcga ttggggctaa gattaaagaa gcctacttag cacgtgattt atttttcgta
    83521 ttagaagatg aaaaaacggt ggctaaattg cagccagatt ttacagctat caaaaatttt
    83581 gaattaggaa taggagtcat cgtgacagca gaaagtgtgc aaaaagactt tgtttctaga
    83641 accttctttc caaaattaac aatcaatgaa gatccagtat gtggatcagc acattctaat
    83701 cttattcctt attgggcaga aagaatgaat aaaacaaaat tgtcagctta tcaaatgtca
    83761 tcaaggggcg gggatctaga atgtgaatta aaagaagatc gtgtgataat aggaggaaaa
    83821 gcagttttgt tttctaaagg tacctcgttt atttgatttc tttcaaatga attaaaaata
    83881 aattggtgga gagtggcaac cttatagcta caatggttgt taaaaaaaga cgcaaacttt
    83941 ttcaatttaa tgaaaagttt gcgtctcatt ttaattattg gtgtttgttt tttattcaat
    84001 caagagtaaa atgccccata ctaactatcc tcggatgact tcgattttat aaccatcagg
    84061 gtcaatgaca aagtagtaag aaggcgcagt gccaggtaat ccttttaagt cagtaatctc
    84121 aagaccagca gctttatgtt gctcgtgtaa tttttctaga tcatcagctg ctatagcgat
    84181 atgaccatag ccgtctccta gatcatacgc accatgatca tagttgtaag tcaactctaa
    84241 ttcatagtca tccccaggaa gtgtcaaata aacaagtgta aatttgtttt ctggaaaatc
    84301 acggcggcgg ctttcttcaa aaccaaatgc cttttgataa aattcgattg atgcttcaag
    84361 atctttcact cgaacacatg tgtgagccat tttcatagat tcatctctcc ttttcctttt
    84421 actcaatcat aacaaaaaga agtaaggaaa tagttgattt gtgcttgttc atgagaaatt
    84481 tctcatgtaa aatggataga aaataaaact gttaaggtgg aaactattca tggataaacc
    84541 aatttttggt gaaaaaaagc aatttgctga ttatcaaacc cgttatgcag catatattgt
    84601 catagaaaga gatcagcaga ttgcagtaat cgaggctcca aatggtgctt tttttcttcc
    84661 tggcggggaa attgaaggag aagagacgaa agaagaagcg attgtccgtg agctgataga
    84721 agaaatggga attgtggcag aaatttcttc ttatctggga gaagctgatg agtattttta
    84781 ctcgaattat cgtgcaactt attattataa tccaggatat ttctttgttg ctgattcttg
    84841 gaagcgtata ggagaaccaa ctgaaaagac caataaaatt tggtgggtga gccctcaaga
    84901 agcgcttgtg aaattgaaac gtggtagcca ccgatgggca gtcgagagat ggatcgaaaa
    84961 aaataaaaac tgaaagaaag cgagtagatc agctttctct cagttttttt atttattaca
    85021 gtacatattt tttgattgct tcagctacac cgtgctcgtc gtttgatgaa gtgatgacat
    85081 ttgctgcttc tttgacaaga gaaacggcat ttcccattgc tactcctaga ccagcatatt
    85141 caatcatgga taagtcgttc tcgttatctc cgatagccat gatttcatct tgatcaatac
    85201 caaggtgctg tgcgagattc gcaactgccg cacctttact tgcttcttta tttagtactt
    85261 cgaagtaata gggcgcactt ttgaccgttg tgtatttatc acggaaagtt tgcggtaaac
    85321 gcgcaattgc tgcatctaaa atttctggtt cgtcgatcat catcattttg acgatgttca
    85381 tgtctggtgt catttcttca ggtgttcgat attttagcgg catcttgact aaacttgctt
    85441 cgtggatcgt ataagcgctg atatctcgat taggcgtgta gatcgctttg tcatcgatcg
    85501 aatggagatg actgccgaca cggcgtgcca ttacttcgat ctctaggaaa tcttcatgtg
    85561 tcagtccgtg acgggcgatg attttacctg atttcgtttg ttgtacgagt gccccgttat
    85621 aagtaataac gtaatcatta tcttgaaaaa ggttcaactc gtttaactgt tcctggacac
    85681 ctggtaaggg acggccagtg cataaaacga tcgaaacacc ttttgcaaca gcagcggcaa
    85741 tcgcttcttt tacttctttt gttacttctc ttttctcatt taataaagta ccatcgatgt
    85801 cgatagcgac tagtttgata gacataattt cctccaatat attttaattt atttcaatca
    85861 atgcgccatt acggatatgg ctggcaaatt cttgataagt ttcgtcaaat aaatcataat
    85921 gatcacgcaa ggacggatca accatttctt ttggaaagaa aaatcgctca tctccttgtt
    85981 cttgtcccgc taatgcagca acaagctgac taacctgtga taattctatc aaagttccgt
    86041 catttcgcaa aatctcgatt tgtgtgcgat gtctgccttg ttctgggcga taaaaatcat
    86101 aaggtagatc gtagctagaa ttcacagcag tgtaataaac aggattatat ccgacctttt
    86161 ctactaacaa agtgagttct tggactaatt ttgaatcccg gttgccagaa aaagtagcag
    86221 atttcagtgg tttacgattc aaaaaacgtt tagctaaatc atttaaaata ggatcagcag
    86281 attcagacca ttgagtaaag taagtgccca agaccccatc atcaagtttg aggtattctt
    86341 ctaaagtaaa gcttccgtca agaaatggca gaagtaattg agaatggagt tcaaaactgc
    86401 tggcatcttc ttgatacaac tcgtgtgcgc gatgaagcag atgctccaaa attacttcca
    86461 taccacggga aacaggatgg aagtacactt gcacatacat ttgatatcgg ctaacgatat
    86521 aatcttccac tgcatgcatg ccactcatgg aaaaagcgat cccgccttca taaggacgaa
    86581 tcacacgtaa tattcgggta agatcaaatg ttccatattc tgttccagtg aaataagcat
    86641 cccgaagcaa atagtccatt cgatctgcat cgatctgact agaaatcatt tggacaactt
    86701 gtgggttggg gtaagttttt tggatcacac tagctacctt ttcaggaaaa ccttcttcta
    86761 ctcggttaag aatctgatac acttctgttt ctggagaagt gatgatctct acagtgatgg
    86821 cttcatgatt cgtatggaag atatgttcaa atgtatgaga gtaaggacca tgaccgacat
    86881 catgcaacag tgccgcacat aaagtcacca gccgttcctg atcatcccag cctccgttgc
    86941 ctattttttc ttttgaaaaa tttcgagaaa agatatcgca gattcgtctt gaaatctcat
    87001 aaacgcctaa tgaatgagta aaacggctat gttctgctcc gtgaaaagtg aaagaagaag
    87061 tgcctaattg tttaatgcgt cgtagacgtt ggacttcttt cgaattgatc aaatctaaga
    87121 ttacttgatg ctggacatgg atatagttat ggactggatc acgaaaaact ttttccatag
    87181 gcagcatttg atatttataa ggggttgtca taatgtagct cctttatgtt attacgttgt
    87241 tccattctct ggatttggtt gttccattgc tccagctgga tttctttttt tagccagtcg
    87301 gtagaatcct gtaaatcact tgcagaatac agcttttcaa aagcttttgc aaatgcttct
    87361 ttgacttctt ttacaaaaag gggttggtca agcagttctt caagtgttgc cattgtagca
    87421 ggatccactg caggatagcc atttgtacca aagtcttctt ttaaagcaga ttgataaaat
    87481 cgcttcatga cttgcccacg tttttcctga ttaccgttga cacttaagta catcataaca
    87541 gaaatcccat ttttgatccg acgctgagcg ataccggcaa attttttgcc atggatgctt
    87601 aaatcataag ttccaggaca ataagaatgg gtgatttcga atgcttgtat cggtgccttt
    87661 ggaaaagcgt atttagtcaa taggagcatt tgctcgtaag cgtcatctat ggttattttt
    87721 tttgtgtcac tttgggggaa aatcaaagaa atattcaaaa ttccttcatc tgatacgatc
    87781 cctaatccac ctgaatttct gatcactgga gcgtaacctg catcttccag tgtttggatc
    87841 ccgtcactta gaaaaggagt tcgaacatcc ttcataccta agatgactgt gcagtcatat
    87901 tgccagaaat gtaaaaagat ttgctggtgt tctttagaat attccgttaa tatatctgtt
    87961 aatgcaaaag gggaaaagtc gtttttttga taaatgtgct gatctagtcg aatcgcaggg
    88021 ttcattcaac cggctccttt gtataaattc atattttcta caatattata ctctaaaatc
    88081 aaagagttgt tttggaaaaa aggttttctg taaaaatgga caagttggtt gacaaaacaa
    88141 aagaaactag gaacaatgta gctatgagaa gtaaaggaga atgaataatg gaattatcac
    88201 aaggaacacc gatttcaatc aaattgcgta caaaagtaaa acagcaaggg gaagtgcaag
    88261 atttttattt tgatttgaaa gggcaaatgg tgacaattgg cgatacgctg tacatacgtt
    88321 ataaagagat tcaggaggag acgggcgatg agattcctgt aacgatcaaa ttagttccag
    88381 atggtcatgt acagctgatc cgtgcaggag aaatgcggat gcgtttgaaa tttggctata
    88441 aagaacggtt agaaacatcc tatcgaacac catacggtat gatccagatt gctactttta
    88501 cgaaagaact gcatgttagt ttgaaagacc gtcccacagc agggaaggtt cgaatcgatt
    88561 atgatctgtt catgggaccg gaaaaaatcg gcgaatatta tttgaccttg gattttacag
    88621 cgtaaatata aaagaaaagt ttgatttttt gtgtctgatt ttgtatgata aggagtagac
    88681 tgtgaaagga cgtgtcctga ttggaaatta gtgtatttga aggtgcaaac aaaagcgaat
    88741 tatcaatgat cgaagtagca cacgcgatct tagaacaacg tggcgatgtc atggattttt
    88801 ctgatttagc taaccagatc caaaactatc ttgggaaatc agacagtgaa attcgtgatt
    88861 cactcgcaca attttatact gacttgaata ttgacggcag ctttatctca ttaggggata
    88921 atcgttgggg cttgcgttca tggtatgcta tcgattcgat cgatgaagaa gtaaaccatg
    88981 gaatggacga agaggatgaa gatactccac gtcgccgtaa acgcaagaaa gtcaacgcct
    89041 ttatcaatga tgatgaagac gcgatcgatt acaatgacga tgacccagaa gatacagatc
    89101 tgacagaaga agatgacgat gatctgtttg atgacgatga tgatgaagat gaagaaatcg
    89161 ctgcttataa ttctgacttg caagaaatcg gagcagatga cccgacagat gatgaagaag
    89221 acttgccagg aaatatcgaa gaagatttaa gtatcataga cgaagacgat gatgatgaag
    89281 attatgaaga ggaagaattt tctgactctg aaaaagaata aacattattt tcttacgaga
    89341 ggttgtgaca gagacgatca gcctatcaga ataaggaggt gtgacacgat ttttgtcaca
    89401 ccttcttttt tgtatataaa aatcccaagt aaatacataa aaaataaaaa aattgaaact
    89461 tttagcgcac cttgtacgtc atattaagtg aggcagaaag gagtggagaa tgatttttac
    89521 tggatataag aaaaaacggc agatcgaaaa ataccgtcac ctgctgaatg gactcatcga
    89581 aactgattat caaaaaatgt tccgtatcac gatgaattac gtacatgata aagaagaggc
    89641 cttagatgtg atgcaagata gttttcataa agccctgaac tattttgaaa aagaaaaaga
    89701 gatcgagcat tttagtgcat ggttttatcg gatattgatc cgcacggcat tagatagttg
    89761 gcgcagacaa aaaaggacgc ctgtggactt atttgaaatt caagatatcc cccaacagtt
    89821 acctttagaa acttccgttg cagagctcca tatgatttta gataaaatag acagtccgga
    89881 aaaagagatt ctgatcctct acttttttga aggatttcgt ttgaaagaaa ttgcgactat
    89941 cttggatatg aatgaaaaca cagttaaaac gaaaatgtac cgctcattga atgctttacg
    90001 tcaaatccta gaatgaagga gagaagagtg ccatgtattc ccaaaaagaa aatttaaaaa
    90061 aactaaagaa aacttataaa gaacaagcaa ttcccaaagg gcttaaagag gagatccgcc
    90121 agcgttttat tatcgaagag caatcattta taaaagcaaa gcggagaaag agacgtctcc
    90181 aagggttcgg agtatctttg gctatcgtaa tgattacaac aagtagcctt ctttttaata
    90241 gtcaagttcg aagctttgca gaagatttac ccatcttagg atcagtcatt gaattgatct
    90301 taggcgaacg ctttactgat cgatctgaaa aaatcgatat tcaggtaccg aagatcaata
    90361 cacaaaatga gaaagaaaat aaaacaattc atggattaaa tcaaaaatac tttcgcgaag
    90421 gacaagcaga ttttgaaaaa gctgaaaaag aatatggaaa ttttgaaacg gatcattacc
    90481 aagttttggg agattatcag aaaatcgtag acgataaccg gtttctcgtg attgaaaggc
    90541 agatcaccca aacagcagca gatagccatg ttgaaaaaag atacgacaca atcgacaaga
    90601 aaaacagtgt ccagctctca cttccacttt tattcaaaga tgacacctat ctatctgttt
    90661 tgacaaaaga agtcaaacgg cagatggccg aacaagtcaa ggaagatcca tctaagtact
    90721 attggactga gcaagatatt caagaaggga cgatagaaaa accaacactt gttacgccta
    90781 cacgaagttt ttatttgaac aaagagcatc aattagtttt gactttttct caatacgaaa
    90841 ttgctcctgg ttatatgggt acaccagaat ttgtcattcc taagtccgtg acaaaaacga
    90901 ttttggcatc ggaagattat ttggatcatt agacgagaag tagttgcgac tagcagtttc
    90961 aattttcaag taagtacaag taaataataa gaagagaaaa agagaaagaa gagatatagt
    91021 gaaaagaaat tatttagtta tcagtattat tgctttatcc gtgcttttgt ttttcgaaat
    91081 tacttcggtg gcctccttga tcaccgaagt aaaaggacaa acacactctt cggagcattc
    91141 gtcacaatct ttcaaacaat cagaacaatc aaccattaaa agccaatcaa ctgaaacagc
    91201 taacgtcaaa gaagccattc aagaatcagc ggaaaaggta caaagtcagt catcacaatc
    91261 aacacaagac agtacaaaaa cgagccgaga tccggctgtc aaaagcgtcg caatcagctt
    91321 tgatgatgga ccaggagcga ccacaacacc gcagcttctg cgaatcttaa aagaaaaaaa
    91381 cgtgcatgcc acttttttcg tcttaggaga aaatacggcg cagcatcctg aaatcgtgaa
    91441 gcagacagct gaagcagggc acgagattgg caatcacaca tacgatcatc agaatctggc
    91501 cactctttct gcccagtcga tgacggagga agtgacgaaa gcagacactg agatcaaaaa
    91561 agtcactggc aaaaccccca cttttgttcg tccgccctat ggttctgtca cgaatgtagg
    91621 agcaacgatc atccagcgac cgatcatcga atggtctgtt gattcagaag attggaagac
    91681 aagaaatcct gatttgattt tgcagaagat ccaagcaaca gtatacgatg gggcaattat
    91741 tttattccat gatatctatc ctgaaacgat tcgtgccgtt cctcaagtga tcgattatct
    91801 gaaagagcaa gggtatcgca tcacgacagt tggggattta ttagggcatc caacggctgt
    91861 cgagaactat tatggcagaa acgatcatcg gccagttcag taagtgagac aagtatcttt
    91921 aataaataac acattttaat aagtagttga acacataact accttacttg caaaggctta
    91981 acaatatcta tacaggtaca acaaaaaact gtccaccaat ttt
//
LOCUS       DASJTC010000002        72956 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.002, whole genome shotgun sequence.
ACCESSION   DASJTC010000002 DASJTC010000000
VERSION     DASJTC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.002"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(8..1582)
                     /locus_tag="U4B19_000094"
     CDS             complement(8..1582)
                     /locus_tag="U4B19_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN2075866.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(1837..2346)
                     /gene="mreD"
                     /locus_tag="U4B19_000095"
     CDS             complement(1837..2346)
                     /gene="mreD"
                     /locus_tag="U4B19_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEN2075867.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(2350..3204)
                     /gene="mreC"
                     /locus_tag="U4B19_000096"
     CDS             complement(2350..3204)
                     /gene="mreC"
                     /locus_tag="U4B19_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEN2075868.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(3359..3997)
                     /locus_tag="U4B19_000097"
     CDS             complement(3359..3997)
                     /locus_tag="U4B19_000097"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEN2075869.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3998..4648)
                     /locus_tag="U4B19_000098"
     CDS             complement(3998..4648)
                     /locus_tag="U4B19_000098"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEN2075870.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4661..5560)
                     /gene="rsgA"
                     /locus_tag="U4B19_000099"
     CDS             complement(4661..5560)
                     /gene="rsgA"
                     /locus_tag="U4B19_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEN2075871.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5725..7794)
                     /gene="pknB"
                     /locus_tag="U4B19_000100"
     CDS             complement(5725..7794)
                     /gene="pknB"
                     /locus_tag="U4B19_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEN2075872.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7791..8531)
                     /locus_tag="U4B19_000101"
     CDS             complement(7791..8531)
                     /locus_tag="U4B19_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEN2075873.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(8544..9902)
                     /gene="rsmB"
                     /locus_tag="U4B19_000102"
     CDS             complement(8544..9902)
                     /gene="rsmB"
                     /locus_tag="U4B19_000102"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEN2075874.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9902..10840)
                     /locus_tag="U4B19_000103"
     CDS             complement(9902..10840)
                     /locus_tag="U4B19_000103"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEN2075875.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10833..11324)
                     /gene="def"
                     /locus_tag="U4B19_000104"
     CDS             complement(10833..11324)
                     /gene="def"
                     /locus_tag="U4B19_000104"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN2075876.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(11339..13747)
                     /gene="priA"
                     /locus_tag="U4B19_000105"
     CDS             complement(11339..13747)
                     /gene="priA"
                     /locus_tag="U4B19_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEN2075877.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(14000..14305)
                     /locus_tag="U4B19_000106"
     CDS             complement(14000..14305)
                     /locus_tag="U4B19_000106"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN2075878.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(14310..14924)
                     /gene="gmk"
                     /locus_tag="U4B19_000107"
     CDS             complement(14310..14924)
                     /gene="gmk"
                     /locus_tag="U4B19_000107"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEN2075879.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(15411..15695)
                     /locus_tag="U4B19_000108"
     CDS             complement(15411..15695)
                     /locus_tag="U4B19_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075880.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15713..16603)
                     /locus_tag="U4B19_000109"
     CDS             complement(15713..16603)
                     /locus_tag="U4B19_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEN2075881.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16623..16841)
                     /locus_tag="U4B19_000110"
     CDS             complement(16623..16841)
                     /locus_tag="U4B19_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEN2075882.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16811..18511)
                     /locus_tag="U4B19_000111"
     CDS             complement(16811..18511)
                     /locus_tag="U4B19_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEN2075883.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18778..19551
                     /locus_tag="U4B19_000112"
                     /pseudo
     CDS             18778..19551
                     /locus_tag="U4B19_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19632..20201)
                     /gene="thiT"
                     /locus_tag="U4B19_000113"
     CDS             complement(19632..20201)
                     /gene="thiT"
                     /locus_tag="U4B19_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEN2075884.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(20267..20369)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20375..21346)
                     /locus_tag="U4B19_000114"
     CDS             complement(20375..21346)
                     /locus_tag="U4B19_000114"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN2075885.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(21440..22813)
                     /gene="glmU"
                     /locus_tag="U4B19_000115"
     CDS             complement(21440..22813)
                     /gene="glmU"
                     /locus_tag="U4B19_000115"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEN2075886.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(23316..24134)
                     /gene="purR"
                     /locus_tag="U4B19_000116"
     CDS             complement(23316..24134)
                     /gene="purR"
                     /locus_tag="U4B19_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEN2075887.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(24301..25125)
                     /locus_tag="U4B19_000117"
     CDS             complement(24301..25125)
                     /locus_tag="U4B19_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2075888.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(25098..25778)
                     /locus_tag="U4B19_000118"
     CDS             complement(25098..25778)
                     /locus_tag="U4B19_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2075889.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25801..26739)
                     /locus_tag="U4B19_000119"
     CDS             complement(25801..26739)
                     /locus_tag="U4B19_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN2075890.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26984..27847)
                     /locus_tag="U4B19_000120"
     CDS             complement(26984..27847)
                     /locus_tag="U4B19_000120"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEN2075891.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(27989..30562)
                     /gene="mprF"
                     /locus_tag="U4B19_000121"
     CDS             complement(27989..30562)
                     /gene="mprF"
                     /locus_tag="U4B19_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN2075892.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30703..31509
                     /gene="proC"
                     /locus_tag="U4B19_000122"
     CDS             30703..31509
                     /gene="proC"
                     /locus_tag="U4B19_000122"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEN2075893.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(31588..33144)
                     /gene="rny"
                     /locus_tag="U4B19_000123"
     CDS             complement(31588..33144)
                     /gene="rny"
                     /locus_tag="U4B19_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEN2075894.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(33555..34604)
                     /gene="recA"
                     /locus_tag="U4B19_000124"
     CDS             complement(33555..34604)
                     /gene="recA"
                     /locus_tag="U4B19_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEN2075895.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34713..35954)
                     /locus_tag="U4B19_000125"
     CDS             complement(34713..35954)
                     /locus_tag="U4B19_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEN2075896.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(36194..36772)
                     /gene="pgsA"
                     /locus_tag="U4B19_000126"
     CDS             complement(36194..36772)
                     /gene="pgsA"
                     /locus_tag="U4B19_000126"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEN2075897.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36981..37904)
                     /locus_tag="U4B19_000127"
     CDS             complement(36981..37904)
                     /locus_tag="U4B19_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2075898.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(38643..39929)
                     /locus_tag="U4B19_000128"
     CDS             complement(38643..39929)
                     /locus_tag="U4B19_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN2075899.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39922..41190)
                     /locus_tag="U4B19_000129"
     CDS             complement(39922..41190)
                     /locus_tag="U4B19_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEN2075900.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            41442..41903
                     /gene="mscL"
                     /locus_tag="U4B19_000130"
     CDS             41442..41903
                     /gene="mscL"
                     /locus_tag="U4B19_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEN2075901.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41962..42408)
                     /locus_tag="U4B19_000131"
     CDS             complement(41962..42408)
                     /locus_tag="U4B19_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2075902.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(42501..42746)
                     /locus_tag="U4B19_000132"
     CDS             complement(42501..42746)
                     /locus_tag="U4B19_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEN2075903.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42861..43439)
                     /locus_tag="U4B19_000133"
     CDS             complement(42861..43439)
                     /locus_tag="U4B19_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2075904.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(43516..44046)
                     /locus_tag="U4B19_000134"
     CDS             complement(43516..44046)
                     /locus_tag="U4B19_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEN2075905.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(44050..44910)
                     /gene="rlmB"
                     /locus_tag="U4B19_000135"
     CDS             complement(44050..44910)
                     /gene="rlmB"
                     /locus_tag="U4B19_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEN2075906.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44903..45298)
                     /locus_tag="U4B19_000136"
     CDS             complement(44903..45298)
                     /locus_tag="U4B19_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEN2075907.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(45295..46707)
                     /locus_tag="U4B19_000137"
     CDS             complement(45295..46707)
                     /locus_tag="U4B19_000137"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEN2075908.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46704..47240)
                     /gene="cysE"
                     /locus_tag="U4B19_000138"
     CDS             complement(46704..47240)
                     /gene="cysE"
                     /locus_tag="U4B19_000138"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEN2075909.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(47326..47565)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(47562..49022)
                     /locus_tag="U4B19_000139"
     CDS             complement(47562..49022)
                     /locus_tag="U4B19_000139"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEN2075910.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(49192..50349)
                     /locus_tag="U4B19_000140"
     CDS             complement(49192..50349)
                     /locus_tag="U4B19_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEN2075911.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(50383..51753)
                     /gene="radA"
                     /locus_tag="U4B19_000141"
     CDS             complement(50383..51753)
                     /gene="radA"
                     /locus_tag="U4B19_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEN2075912.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51816..52343)
                     /locus_tag="U4B19_000142"
     CDS             complement(51816..52343)
                     /locus_tag="U4B19_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEN2075913.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            52573..53379
                     /gene="proB"
                     /locus_tag="U4B19_000143"
     CDS             52573..53379
                     /gene="proB"
                     /locus_tag="U4B19_000143"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEN2075914.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            53376..54623
                     /locus_tag="U4B19_000144"
     CDS             53376..54623
                     /locus_tag="U4B19_000144"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEN2075915.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54699..55043
                     /locus_tag="U4B19_000145"
     CDS             54699..55043
                     /locus_tag="U4B19_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075916.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(55160..55486)
                     /locus_tag="U4B19_000146"
     CDS             complement(55160..55486)
                     /locus_tag="U4B19_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075917.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(56101..57303)
                     /locus_tag="U4B19_000147"
     CDS             complement(56101..57303)
                     /locus_tag="U4B19_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2075918.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            57420..57935
                     /locus_tag="U4B19_000148"
     CDS             57420..57935
                     /locus_tag="U4B19_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2075919.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(58134..58580)
                     /locus_tag="U4B19_000149"
     CDS             complement(58134..58580)
                     /locus_tag="U4B19_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEN2075920.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(58627..60105)
                     /gene="msr(C)"
                     /locus_tag="U4B19_000150"
     CDS             complement(58627..60105)
                     /gene="msr(C)"
                     /locus_tag="U4B19_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN2075921.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(60459..61088)
                     /locus_tag="U4B19_000151"
     CDS             complement(60459..61088)
                     /locus_tag="U4B19_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2075922.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(61072..61536)
                     /locus_tag="U4B19_000152"
     CDS             complement(61072..61536)
                     /locus_tag="U4B19_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2075923.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(62023..63291)
                     /locus_tag="U4B19_000153"
     CDS             complement(62023..63291)
                     /locus_tag="U4B19_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN2075924.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(63522..64259)
                     /locus_tag="U4B19_000154"
     CDS             complement(63522..64259)
                     /locus_tag="U4B19_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2075925.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(64326..64895)
                     /locus_tag="U4B19_000155"
     CDS             complement(64326..64895)
                     /locus_tag="U4B19_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN2075926.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            65023..65895
                     /locus_tag="U4B19_000156"
     CDS             65023..65895
                     /locus_tag="U4B19_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2075927.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            66237..67109
                     /locus_tag="U4B19_000157"
     CDS             66237..67109
                     /locus_tag="U4B19_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2075928.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            67106..69064
                     /locus_tag="U4B19_000158"
     CDS             67106..69064
                     /locus_tag="U4B19_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2075929.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(69396..70247)
                     /locus_tag="U4B19_000159"
     CDS             complement(69396..70247)
                     /locus_tag="U4B19_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2075930.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            70370..70837
                     /locus_tag="U4B19_000160"
     CDS             70370..70837
                     /locus_tag="U4B19_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEN2075931.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(71100..71582)
                     /locus_tag="U4B19_000161"
     CDS             complement(71100..71582)
                     /locus_tag="U4B19_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEN2075932.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(71682..72545)
                     /locus_tag="U4B19_000162"
     CDS             complement(71682..72545)
                     /locus_tag="U4B19_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN2075933.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        20414 a        15633 c        13091 g        23818 t
ORIGIN      
        1 atcattctta catgctgaca gcaaagtcag gggcaaacca ttgaactgaa ccaactttta
       61 cgctttcgcc tggttgagga gcatggatat attgtcctcc gcctaaagca atcgctacgt
      121 ggtaagttcc gccttgtgac ccccagaata acaagtctcc agcttttgct tgagaaactg
      181 aaatttttgt tccagctgat tcttgaggaa ctgtccaacc accgatgtca cgaccagtta
      241 cttgcatgta aacatagcgt gtgaaccctg agcagtcaaa gccacttgga tctttaccgc
      301 cccaaacata tggagtacca atatatttgt aagcttctgc tacgattgct gcaccattca
      361 cacttccaga aggagctgga gcaggtgtta caggttttgg ttgttcaggt gttggtgttg
      421 gcgttggagc cggagtacta cttcctgtgc cgtttcctgt atcaacactt tgatctgttg
      481 atggtgttgt aggcgcaggt gttgttgatt cttctgttgt tgaagattca ggcactgttg
      541 ttgattcttc tgttgctgaa gattcaggca ctgttgttga ttcttctgtt gctgaagatt
      601 caggcgctgt tgttgattct tctgttgctg aagattcagg cgctgttgat tcttctgttg
      661 aagattcagg tgttgttgat tcttctgttg ctgaagattg tgtagctgat gatgattctt
      721 cagtcattga agattgtgta gctgatgatt cttctgttgc tgagctttgt gcagtagatg
      781 aagcttgagt agcttgtgct tcagcctctg cttgttcgcg tgcttctttt tcagcttttt
      841 cttgtgcagc ttgttgacga gcttgttctg ctagacgttg ttgttcacga atacgagctt
      901 gctctgcttc agcttctgct ttttgacggt tcaagtcagc tttcttgtct tcagcagttg
      961 cttgttcagc agctaatgaa gttttcagta cgttcaaatc agcttgttta gataaaagat
     1021 cgcctttttg agattccaaa gcagcttggt tttcagctaa ttcttttagt ttagcatcgt
     1081 tttcagcttt tttgtcttct acagcttttt tatcttgttt ttgttgttcc atcaagtcgt
     1141 tgtttgcttt taccatcgtt gtcattgctt gaacgcgtcc aatagcatct gctaatgaat
     1201 cagcattcaa tacagcatcg atgtagtttg agcttgtgtt gcttacttgt gcttcgcgtg
     1261 cttgtttttg gattgtatct tcacgttttt caatacgttc ttgtaaatct gcaatgtctt
     1321 ttactaattg tgcagattct tgacgcaatg tatcttgttt tgctaatagg tcttgtgctt
     1381 gtgtgttgat ttcagaaact tggctttcta aggcatcgat ttgtgattgt gcatctgctt
     1441 gttgattttt caagtctgca atcttttgat cttgctgttg tatctgagaa tcaaaatcgt
     1501 ccgccgcggc agcgatcggt gatgctacgg cagtcaaggt cattgaacat accattactg
     1561 ctgatattaa actctttttc actcttcatt cctccgactg gcttaattta ataaatatct
     1621 aaatatattt aagtctttct taacgatata aaaattgtac catagcagtg tgtcaacgat
     1681 ataatagtta tgttacaaaa atgtttcaaa acaactttta aaaatgacaa aactgttcgt
     1741 ttcagaaaat gatcatgaaa aagccgcgtt aagtctagca tttcttaaga taacaacgat
     1801 tctttttcgc tttcgaccta aaaataaaaa aagaaattat tcatcactga ataatttctt
     1861 aaagggaaaa atgaataata caaatataat catgttcaac agcagtgtag gtcctaaaaa
     1921 ccttgtaata aaatacgtgt tatttactac tgctaattta aaaatcaact ggaccaccat
     1981 cgtaaacaat tcataacccg tgacaaagat gatcatactg aaaaattcag taaagatgtt
     2041 gacatggata acgtctttca ttacatacat caaccataca attaaaggga gagctactgc
     2101 atagataccg ataacgccaa tatagtaagc gtcatagata gccccgagaa caatcgtggt
     2161 aatcagtaaa taacgttttt caaacgccat actgcaacat aataatgcca atatcagaaa
     2221 gtgggcattt gacatatagg tgcctttcga ccactcccct agcattcttg ttaaatgacc
     2281 atctatcaac atcaaaaaga acaaaacgat tggcagatag tacttcattg tttctttctt
     2341 caacatggac taatcctccg ctagccgttg gacaatcgtc acaactgaca cgtcatacat
     2401 ttctgcataa ggtttcacat aaacttcccg atccagccca taactatctg gtttcgtttt
     2461 aataaccgta ccgatcggca agttagctgg agaattccct ccaagaccgg atgtctggac
     2521 aacatcccct tctttgatat ccgtatcacc agttaattgg gtcaccacta aggcatggag
     2581 cttttcatca tagtttttaa gcaaaccaaa cgcttcgcca ttagccgaag atacccgtac
     2641 tggaaaatga ttggagcttt cattagagga tgacagtaat tcgattttag acgaagccgt
     2701 attgacctcg atcactcggc caatcaatcc tttttgcgac atgactgcca tatttgcttc
     2761 gataccgtct ttagaaccct tgtctacgat cagcatgtcc tgccaactgt caggcgaacg
     2821 agtaatgaca tttgccgtta ctttttcgta actagtcagc gtctgattca actcaagttc
     2881 ttttttcaac gcttcgattt ctctctcgta attcttgttt tgctgtgaaa cgtcatcata
     2941 gctatcgatt ttttctttta gccgctcatt ttcggcataa gttgaaaaaa gattatggac
     3001 agaatctact ccatttcgta cccaccgtac tggggcagag attgttttat caaccattgc
     3061 aacactgtca ttaacgatgg attggaagaa attgggcttt ccttcattcg ctcgctgcgc
     3121 agcggtcaca cttagtatcg tcaccacgat gataacaata accaaggtaa taataatatt
     3181 tttattagga ttgaacttct tcacaaagac acctcgaatt aattagactc ttgtgttcaa
     3241 ttttatcact aaatgccgtg aaacaaaagg taagttgctc ctcttaagct tttttttagc
     3301 aaataataga ttctgtcatt tttagcctga aagacaaatt tttttatttg tttatggatc
     3361 aatctttgct ttgaataact gcaatcatac catctgaaaa agaaaattct gcggtatctg
     3421 aaagaaattc gttacttgca taagatgtcg gatattctac ttctttattt tccaatacat
     3481 acttgctgcc ttttaatgta agattcgaga cgggagtcaa gcagcaataa gctaaatact
     3541 tcatatctgg tatctgtctt actatatgct ctccaggcaa ataataagac aaataatttt
     3601 gacaatctct gataataagc tgatgggcaa agggtttgaa gcgaggttcc atacctaacc
     3661 agagattggc taaaagatga tccaatcttc cgcccgtagc tccaatcaaa gtgacttccg
     3721 cttgcgggta acgttcaaat gtttttaaga tacctaactg tgtatctgta tcgtcttttt
     3781 ctgcagggga acgggtatac tctttggctt ttgcaaatac ttccttctgc tcttcgttcg
     3841 acaatgaatc aaaatcaccc actgccagat ctactgggta gcctgcttga agcaggtaca
     3901 gcgcaccacg gtctatcccg acataggcat cgtatttgga cagcagagac tgcggccagt
     3961 tttgcggatt acctccagca gctaataaaa ttttcattta gtctaatgcc tcttttaatc
     4021 gttcgatttg tccgactgga ttttcggctc catagatata tgatcctgca acgaagacat
     4081 ccgcccctgc atctttacag atttttgctg tttctggaac gattccgcca tctacttcga
     4141 ttgcataagt ccaattgttc tctctacgta gatcagcgag ttccgtaatt ttttctactg
     4201 tttcttcaat aaagctttgt ccgccgaacc caggattgac agtcatgact agtacttgat
     4261 cagccaaagg caaaacatgt ttgatcatgg agactggtgt tcctggattg ataactactc
     4321 cagctttaac tcctaagctt tttgtcattt gaagtgcccg atgaatatga ggagttgctt
     4381 cgacgtggac agtaatgata tctgctccag cttttgcaaa tgctggaatg tagttttctg
     4441 gatcaacgat catcagatgg acgtctaacg gtaatttagt aacaggacgg attgcctgaa
     4501 caatatttgg ccctaatgtg atatttggga caaatcggcc atccattaca tctacgtgga
     4561 tgtaatctgc gcctcctttt tccactaact caatgtctct ttgtaaattg gcaaaatctg
     4621 cactcaaaat ggatggtgct atcttcattc ttgtttcctc ctattttcgt ttaccttctt
     4681 tttttgcata cattggtttt cttttttcga tttcttgtaa gaattgcaaa taattgtcgt
     4741 aacgtgtctg tgcgatacca cctgtttcta ctctatgttt tacttcgcag ccaggttctt
     4801 tagcatgcat gcactctcta aatttgcaat ggacagaagc ttctacaaat tcaggaaatt
     4861 gtctcggcag ttctcctgct tcaattgtca aaaagtcaat cgagctaaat cctggtgtat
     4921 ctgctactaa cccgccatag ataggaatca gttccacatg acgtgttgta tgccgtcctc
     4981 ttccaagtgc atcagaaatc tctcctgttg ctaaattcag ttccggtgat atttgattca
     5041 gtagcgtcga ttttcctgct ccagactgac ccataaagac cgatagccgt tctgggaagt
     5101 aacggatcag ttctgtcgta tcacccgtat gattgggtac aatcaccgga tagcctattt
     5161 tagtataggt ttgcttgatc tcttcgattt tttctgggtc gtctgacaag tcggtctttg
     5221 tcaaaaaaat aatcggttcg atcgactcgt attccaaggt aactagaaag cggtcaagta
     5281 gattataaga aaatgcaggc tctacgatac ttgtgacaat gattccaaga tcaacatttg
     5341 tgactggtgg gcgaactaat tgattttttc tcggcaagat ttccagaaga tagccgtctg
     5401 tttgattttc actctcaaag atcacctgat ctccgacaag cggcgtgatt ttccgattcc
     5461 ggaaatttcc tcttcctctt gtttgatagg tctctccgtt tgcgtataca tagtaaaaac
     5521 cgctgattgc ttttctgatt tgtcctttga taagtgccac ctccgttttt gtttatttta
     5581 ccatacgaag aggaaagtag gtgttattcc atgcaaactt ctcaaagtat atggcttgaa
     5641 aattgatcaa aagagataga aacacaaaaa agagaatgca acaaatcaat gtcgcattcc
     5701 cttctgatat attttattca gcacttattt tgaactttct gaggtgctag ctgatgaact
     5761 tgtggagctg gaagattctt cttttgaaga gttgcttgtg ctactggagc tacttgagct
     5821 gctagaactg tctgaagagt ctttttcaga actagaagac gctggatctg gtcctttaga
     5881 gtaaattacg ttcacaacca ttcctttggt aattgtcgtt cctgcccctg gtactgtacg
     5941 aatcacttta tctttatcca ccgtgtttga atattcggaa gtctcatgcc cgatatattc
     6001 tgcaccgaca ctagctaaat aactttgtgc ttcgcttttt gtatagccgg agatatcatt
     6061 caatttaggc tctgttcctt ggcttacgac aaaattgatg taagtttctt ctgctacaac
     6121 ttcacccgag gctggatcct gactgatgat cgaacctgat ggtacagtgt cgctatagtc
     6181 ataagtagca ctaatattat tgatattgaa tccttgtgta acaagccaat tacgaacagt
     6241 ttcgtagttt tctcctacat gactactcaa agtcactgtt tctggtcctt tgctgactgt
     6301 gaaagtaatc gtgtctgttt taggatcgac ttctgtatct tctgatggag attgcgaaat
     6361 gatcattccg ctttctacct ctgaactgaa ttcctcttct ttttgaatca agtcagcaga
     6421 aaaccctaat tctttcaaac ttgcttcggc atcttcataa cttttaccac tgtaatcatc
     6481 taatttgatt tttttcgtgc cgctactaat atacagagtg acttcgcgtt tttgcttgac
     6541 tgtcgttccc gctgccgggt ctgtcttcac tacattacct tcttcgattt tgtcatctgc
     6601 aatttcttct gtttttgttg ctacttctaa tttcgctttt gcgagtgttt ctctagcaga
     6661 agcttcagaa agatcagaaa catcaggaac aacaacttct cctcgcccgc cagaagcaaa
     6721 gaatacagct gttccgattc ctaaaacagc aagaatagct aataggatca gccaccattt
     6781 tttctgtcct ttctttttag gatcaggcag aacttcttcc tcctctggta aggtttcgtt
     6841 ttctggcttg tcttttggca gaggcataga tttgaatgaa tcaggcattg gtgcatcttc
     6901 cgtaaggggt gtgataactt ttgtttcatt atccatcaca tgaggctgcc atttttcttc
     6961 gtttgcacga gctggtgata atactgtaga aagatcatca gacatttcct cggctgactt
     7021 ataacggtct gcaggttctt tagccgttgc ccgtaaaatc acgttttcca gtgcttgtgg
     7081 cacatttgga tcaagtgctt ttatagatgg aagatcatct tgaaaatgtt ttaaagcaat
     7141 tgttacagca gattcaccat caaacggtac gctacccgtc aacatctcat aaaggatgat
     7201 ccctaaagca taaatatctg actgcttcgt tgccatactt cctctggcct gctcagggga
     7261 cagataatga acagatccta gcatcgtatt cgtctgcgtg atcgatgttt cagataaagc
     7321 gatagcaatc ccgaaatccg tgatttttac aacaccttca ttatcgatca gtacgttttg
     7381 gggtttcaaa tctcggtgga tgatttgatg gttatgagct aaagaaatag cagaaaggat
     7441 ctgctgcata atatttactg ctgtttcata aggaacagga taatgtgttt ggatatatcg
     7501 tttcaagtcc attcctttga cgtattccat caccagatac tgcatgttgt cttcttcacc
     7561 aacatcgtaa acactaacaa tgtttggatg aacgagttct gttgctgcta aagcttctct
     7621 ctgaaatcgt cgaatcgcag tctggtcatt ttgaaagtca aatcgtaaga ctttaactgc
     7681 tacatcacga tccaagatta aatcgtgcgc caaaaataca tttgccatac caccgctacc
     7741 gatatttcca gtgatttgat aacggccatt taattttttg cccaactcga tcatacactg
     7801 tcctccttat attcgataag aagaacggtg atattatccg ctccgcctgc ttcattggca
     7861 cgatcaatca gctcagttac tttatgagac aatgggcctt cttgattgat gatcgttcct
     7921 atttcttctt cagacagcat gttcgtcaag ccatctgaac ataacagcag tcgatctttt
     7981 agttgccaaa gatgagtaga aacatctact tcgactgttc caggcatgcc gacagaacgt
     8041 gttaaaatat ttttccttgg atggttcact gccatttctt cgctgatttc tcctgatttg
     8101 actagttcat tgactaaaga atgatcctcc gtcaattgca tgattttatc ttcacgaatc
     8161 aaataagctc tgctatcccc aacattggca atcgtgaacc agtcatctga cagagcggcc
     8221 gctacgattg ttgttcccat accgttatat tccggctgtt cttgtccacg ctgataaatc
     8281 agcatatttt cttcttgaat cgattgaatg aaccattggg cgattttttc atcgtcaaca
     8341 gcttcttgtt tttcccaagc ttcgcctaga ttcgttaccg ccatctgact ggcaatgtct
     8401 ccggcacgat gaccacccat cccgtctgct aatacagcaa gcttgagacc tgaacggctg
     8461 gtaaatacac tggcatagtc ttgatttgtg ttacgtcgtc ttcctacatc tgattgatat
     8521 tcaatctgca ttttttcacc tcattacttt ttgcgcaaac aacaaatgaa aaatccatct
     8581 gtgtaaaact gatgcggata caaagtcaac attttctctt cgattgatga ttgtagcata
     8641 ggattggtca aaacatcaat cctctcaaaa tcagggtgtc tttccaagaa agcagaaaca
     8701 acgtcttggt tttcttcttt taagatcgta caagtgctat aagtcagaat accggaagac
     8761 tttaaagttg gtgcgcagct ttctaaaatt tccaattgga tttttggtaa attcgctaat
     8821 tcatccggct gttttttata acgaatatct ggttttcgcc gaagcagccc aagacctgaa
     8881 caaggcgcgt ctactaaaat ccgatcaaac tgctctgctt cgaactgttt actgacttct
     8941 ctagcatcca tcttttgagc gtagacagca gaagagacac ctagacgtgc tgcattatct
     9001 tcaatgagtt tgactttgtg ttcgtgtaca tctaaagcga ccacttctcc accgcatgac
     9061 ggatctaaaa aagaagcaat atgcgtcgtc ttacctccgg gtgctgcaca ggcatctaag
     9121 acatgatgtt caggctcgat ttgcatagac ggcgctacaa gcatcgaact ttcatcttgg
     9181 atcgtcatga cgccatcgtg gaacaatgag ctagaagcta aatgcccttt tttcgctact
     9241 ataccgtatg gactgacttg gcttttttca gctgtgatct cttcttcttg caaaacagtg
     9301 atggcctgat ctctggaaag tcggcgtgta tctactcggg cactagcatg gctaggctga
     9361 tatagcgata aaccgagctt ttcagtttct tcgtatccga tttgatccac cagtcgtttc
     9421 gtcatccata gaggcaggct gattttgaca gaaagtcttt cgataggatc agcaatgcct
     9481 tgcagatctg gtgctccttg acgctgataa ttcctcaaga caccatttac aaatttccct
     9541 gtcccaggat ttccttttac ttttgcgatt tcgaccgcct cgtttaaaat agcatgtgca
     9601 gggactttat ctaaaaattc taattggtat acagaaagat aaagaagcat cttgacccag
     9661 tcatccactt tttttgcttt agcgatcaat ggtgcaacgt aaaattccaa tagtcgctga
     9721 tggctaatcg taccatagat caattctgta aataaaccca catctttttg atttagctcg
     9781 ctttgtttga tcatctcatt caacaaaaga ttcgagtagg cgccaccctt gttcactcgc
     9841 tctaatgtca gtaatgccat atatcgtact gattttttca tttttttagg gatttttttc
     9901 attatttcac ctgctgacca atctgaactt gctgaccgct tccgttaagg aaatcatgta
     9961 cggcctgttt tcctttacca gctggctgta attcattgat ggctaagacg gtctgtttac
    10021 cgcaagcaat ccatagattt tttttgtctt tccggatgat cgtaccaggt gctttgtctg
    10081 tagtttctgc caatacttca accgataata atttccagcg tatgccctca taggtcgtga
    10141 atgcaattgg ccaaggacgc attccgcgta cttggttgtc aatttcttct gcagttttat
    10201 gccaatcgat tgcttcttgt tcccgtgtga tgtttggcga aaaagttgct tctgactcgt
    10261 cttgaggtac tggtttttgt ccatccaaaa tattgggcaa tgtttctagc aatagctttc
    10321 ttcctaaaag gctaagcttt tcaaacatcg tccctacatc atcttgttta gtgattggta
    10381 tgctttcttg cgcatagatt ccgcctgcat ccattttttt gatcatttcc atgatcgtta
    10441 caccagtttc tttctcccca ttcatgatgg cataatgaac aggcgcacct ccccgatatt
    10501 taggaagtaa agaagcatgg acattgattg cccctaattt tggtacttgc aataactttt
    10561 caggcaagaa ttggccaaat gccgcagtga cgatcacatc aggcaccaat tcaatgattt
    10621 cttccatttc tggcgaaccg gagattttct ctggctgcag cacacggata ccgtgcttca
    10681 gagccgcttt ttttaccggt gtcggcgtaa ttactcgttt ccgcccaact ggacggtcgg
    10741 gctgtgttac cactgcctgg atctcgtaac ctgattctat cagcccttca agaatcggca
    10801 cagaaaaagc aggtgtgccc ataaatacta gcttagtcat caagatgttc ctccatatat
    10861 tcttcaagtt cttcttccgg gatctgctgt atcattttct caatgaacaa gataccgtct
    10921 aagtgatcga tttcatgctg gaaggcacgg gctaaatagc caaaagccgt cacttctatc
    10981 tcttctccgt ctcgatcata ataacgcacg gtcacttcat ctgctcgctt gaccgttcca
    11041 tagacatgcg gaatactgag acaaccttct acgtcaaggc tttcaccttt tgcttcgatg
    11101 atttctgggt taatcagttc aaatttctcg ccttcatcta cttcgatcac agcaattctc
    11161 ttgttttgtc ctacttgagg agcagcgatc cctattccgt cattcgcaat cattgtctca
    11221 tataaattgt ctaataaagc gattgtctcg tccgtgatta catcgattgg ctgagcagtt
    11281 ctttttaatt tatcattagg gtggatcaat ataggatagc gcataatttg gccctttctt
    11341 aaataaagtt taatggttcg ttgtcaattg ccacaaaaag accttgtcgc tgttctttct
    11401 ggcttccatt caaaatttca tccaaaacat gaggaagatt cggttcatgt ttatatttga
    11461 tgattagttg ataataatat cggtttttca ctctcaaaat catgctgggc gttggcccta
    11521 atagcaagct ttgagggctt aacacttctt ttagctgatt ggcaatctga aagattttct
    11581 ttgccgctac ttgttcttca ggatggctgg cggttatttt cacagtaaag taataaggag
    11641 gatagcctcc acgatgccgt acatgcattt cctgctgata aaactgctca taattttgtt
    11701 ttttagccaa ttctatggcg taatgttgag gattgaatgt ttggatcacg acttcgcctg
    11761 ctttttccgc tcgccctgct cgtcctgaaa cttgggtaag caattggaat gtatgctcac
    11821 tagaacgaaa atccggtaaa ttcagggagg tatcagcatt caacacccca actaatgtca
    11881 cttctggaaa gtccaaacct ttagcaatca tttgtgtccc taataatata tctgcttctt
    11941 ttgcaccgaa tttttgtaaa atctgttcgt gtgccccttt tcttcgagtg gtatctacat
    12001 ccatacgcaa aatcctagct tgcggataaa gttctcggag ttcctcttct actttttgag
    12061 tacctgttcc atagtagcgg attttattgc cgccacaatt aggacagcga ttgggaatag
    12121 gttcttcatg tccgcaataa tggcagcgca ttgaacgtgt atccatatgc agtgtaagag
    12181 agatatcgca attaggacat ggaagaacaa atccacagtc cctacacatc acaaaagacg
    12241 aatatcctcg acgattgagc agtaggacag tttgttcttt tttgtctaat cgattttgta
    12301 ttttttcctg caaattagca gaaaaagtag aggtccgatg attctgaaac tcttcacgca
    12361 tatctacgat ctcaatagct ggaagctgtg cagctgcttt ggctcggtgg tttaactgca
    12421 ataactgata aacgcctttt tgagctcgtg ctcttgattc taaagagggc gtcgcgcttc
    12481 ctagaactat tgggcaatga tgatatttac tgcgccaaat tgctagatct ctcgcatgat
    12541 aacgcggtgt ctcatcttgt ttgtaacttg attcatgttc ttcatcgata atgatcagcc
    12601 cgatattttt cagcggggca aaaatagcag atcgtgcgcc tactactact tgcgcttctt
    12661 cccgctcaat cttgcgccat tcatcatatt tttcaccttg ggaaagtccg ctatgtaaga
    12721 ccgcaactga ttctccgaag cgtcctttga atcgctcgac catttgcgga gtcaatgcga
    12781 tttcaggcac taacataata gccgttttat tttctgacaa aacatcagcg attgcctgaa
    12841 gatatacttc tgttttcccg ctgccagtga ttccctccag caaaaagacc tgatcttttt
    12901 gctgctgacc tgctgtgatg atttgttcga cagcattttt ttgttctgca ttcagttcca
    12961 atgccgtcgt ttggtcaaat actcggtctt tataagggtc gcgataacgt tctgattcta
    13021 taaactccag ccagcccttt tcttttcctt gattcaagac agcagtactg attcctttat
    13081 ttttgaaata agcaattggt tcttcttggc tcagttcaga taaacacaaa agaagttctt
    13141 tttgtttctt ggcattttgc cgtaattttg cccattcttc ttcgatttgc ttttcagtta
    13201 acaaagaacg gaccaaacga atcgttttta ctttatttcg tgtatggact tcatatttga
    13261 tatctactaa ttgctcttgg cgccagcgaa tcaattgaga aaggctgcct tcttcttctg
    13321 cttccttcca ggaaatcact tctcgctgat caaacaatac ctgaacttct ggtgtgtctg
    13381 ccaaaggata gatcaattta tcatattctg ctttcatcac actcggaagc atcgtttgta
    13441 gacaagtgat tttgaatgcg taagtaattt ttttcatata atcagctaat gaaagtaatt
    13501 cttcattgac caccggagca aggtctagca aacgaataac tgccttcaaa gatggattag
    13561 ctgattcttc tgatttttgg atagccataa caaatccttg gacatgacga ttgccattac
    13621 caaaagggac ttctactcgc atcccgactt gcaaagctgt ttccatttca gagggaacca
    13681 aataagtgaa tggctgatcg gtctgcatag taggtacgtc cacaatgact tcagctgtta
    13741 atgtcatggt tcgtttcctc ctttttttgt cttttcttat caagcgacaa aaagttcctt
    13801 gcctatttta ctagagatcg atggatttgt cttatcttct gccaaatcct aacaaaaaat
    13861 taacagcaga taagaaaaaa aagaactgtg tcaaaaagtt cagacacagc cttttctacc
    13921 agacaataag tggcgaatgt ttgaaaagga aacagctggg tttgcttttc tttttagatg
    13981 ttcgctctat ttttctttat tacatttttt gctcttcacg gatacgtgat tccaattcgt
    14041 gttgttcacg ctctttttgt gcttgacgtt cctcttcttc catcttcaaa cgttcacgtt
    14101 ttaattcagg atgtggatca ttgactacat ttcctgcatc gatttcttct aaagcttgac
    14161 cgacgctttt tactgaatca aaagaatcta gagtaggctg tgcacttgca tccaattcat
    14221 gcgcgcgttt acttgctaaa atcactagag aatattttga atttacacga tctaatagtg
    14281 agtcaattga aggttttaac atcataagac tacatctcct ttaacatttt aatatattta
    14341 ccgattacgc gatctactcg aaaatgttca ctggcaataa tgtttttgat tcgttcaact
    14401 gctagcggca cttcatcatt tacaacagca tagtcgtaaa gagccatcat ttcgatttct
    14461 tcttttgcaa ctttcattcg tttttcgatt acttcatctg catctgttcc tcggcctacg
    14521 atccgtgatt ttaattccgc taaatctggc ggagtcaaaa agatgaatac accatcgggc
    14581 actttttctt tgacttgttt tgctccttgg acttcaatct ccaagaaaac atcttttcct
    14641 tcatccaatg tcttgttgac atatgacaaa ggagttccat aataattccc gacatattct
    14701 gcgtattcca gcatctctcc agcttcgatc attgattcaa actcttcttt gcttcggaag
    14761 aaataatcaa cgccttcgac ctccccttca cgcattttac gggtagtcat tgagatcgaa
    14821 tactgaaaat cattatcctc actctcaaaa attgcttttc gtactgtgcc ttttcctaca
    14881 ccggatggac cagacaacac gattaataat ccacgctctg acatgacgac gtcctttcaa
    14941 ctagtctttt tgtacttatc gtttattttg cacttttttt ctgcaacttt caaggttatc
    15001 tttaattata gctagaaatt ggtaaaaaag ctaccctgta ccaagaggtc gtaaaaaagc
    15061 tgtcctgcat caagtaataa gaacagccaa acataaacag cttctcatgt ttttcgtttg
    15121 gttggggtta ttatcgcaga tgcaagcttt tgaacgccgt gtataagagg tcgtgaagaa
    15181 gccgattagt ttcgagtatg aagaaaaatt ttgtgaaaat agtttctcac atttttgcat
    15241 aaatttggct taataccgag aaaatggttt ttgaacatca cttattcaaa ataaaaaaag
    15301 attgtgacac gaattttgtc acaaccttta aatgtattcc tagagcaaag aagctaagcg
    15361 aagaaatcca attctgttta tttattgtat ttctttgttc agcatccttg ctaaaagccc
    15421 atattggaca aagtattcac tacacccatg atctcatctt ttcgagaacg tgctttttct
    15481 gtcggatctt ctacctcttc tgtgtcaatt cgaacaataa atttccgatt catcgtcacg
    15541 taagcaccgt cgttgatcaa aataaacagt tcgttctcat tatagtctgc acaggcatct
    15601 ttccatacgt caaagccgtt tttttcagaa gggatatccc gtttaaccac agtttttcca
    15661 cttaattcgt ggaacgttac gtcataatat ttcattatct ctacctcttt cgtcattcaa
    15721 tattttgtac ttgttctctg attttttcta agatcgtctt agactggacg acttgatttt
    15781 taatttcgat tgcatttgat ttggaaccga tcgtgttgat ttcccgattc atttcttgga
    15841 tcaggaaatc taattcccga cctacaggac attgtgcttc gataagttca tctagttttt
    15901 gtacatggat cgccagacga tccagttcct cgtgaatatc tccgcgttcc aacaagatgg
    15961 ctagctccgt caacaaccgt tgctcatcga ctttcgctcc aagaaattcc tccagctttt
    16021 tttgatacct tatttgaaat tccttttcat agatttctac aaaactatcg agttttttta
    16081 gtacttcttt gaattcttca gtattctttt tcaacactaa agctaaagct tgaccttctt
    16141 tttctcgact cgcctcaata gaatcaagtg cgcttttcaa tgtatctaga gctaactgtg
    16201 ccaattcttc catgctctct tccggttttt cttctaccgt tacgaagttt tcgtccagca
    16261 cgaagcgttc cgccatccga ctaattgaca cttgattttg actaccgtat cgttgttgaa
    16321 ttccgtctgt caattgagtg ataagtgaat caatcaattc ccagttgatg acgatctgtt
    16381 tttcgttttt gccaatataa gctagattca caaatacttc gattcgaccg cggcttaact
    16441 gctgtttaat aagctgacga aaatcgtttt caacaaagtt caggatttta ggacttcgta
    16501 tttgaacatc tagaaaacgc tggttgacac tttttatttc aatatctaac tgatacgctt
    16561 ttgtttcgcg gatggctttg ccaaaaccag tcatactttt cattcgacag ggtccttcct
    16621 cattacatat tttctattgc tttttgcagt tgttcaaact cttcgttcgt caatgtcagg
    16681 cctttcccca ttttttcatg gtctggcgcc caatctcgaa gatcgaattt tggcgggcga
    16741 ccattccagc tgactaggtt cagttcttta cgccacccct tgttgttttc tgacaatata
    16801 gcgatttctt ctacgatttc atatgaaaat tcttgtgcca tggttgttcc tccttgagat
    16861 aatataaata taggatcagt aatgtgtcaa ctggttgctt aagcaattca tgctgcaaac
    16921 gatccttgcg ttttccttgt agcagataat caaaataaac agttttcgtg cgctcatttt
    16981 gtattttttg ctggtatttt cgataccttc gccattcttc agtacttaat tgaccaattt
    17041 gtgccaataa atcatccggc atacttttta tatcttgttg gcagatcatt tgaatcatca
    17101 tgtctacttg atagatttca ttgggcagta aaaaatgttt ttttgctaaa agttctagca
    17161 tcgccaatcc gcatgtctct ggtacgaccc tgttcttata taaattacgc agtttttgcc
    17221 aaaattttat gcttttgaaa aaagataatt ctaactgaat ttcttttgtt aagtaagaca
    17281 aaaatcctcg ccagtatgtt cttgcaggtt taacagaatg aaatgtatac cagttcccat
    17341 agtaatagcc taatcgtttt ttcatttcca gatagcctaa ttgtaaattt aattggttcc
    17401 tttggctatc aaaaagcaga aagctgccta gtgtccacaa tgtttgacat ttccaatgga
    17461 taaaaatgtc tggcatacga atttttttcg ctgggcctgg cccttgcaca tctacgacaa
    17521 atgcctctgt tgctcctttg ttaatcgcaa tatcaatagg tattttgttg cgatacccac
    17581 cgtctgcata tttatttttt ccgatctttc gataagccat agcgggataa aatgaagcag
    17641 aggcgagtat ccaatctggt atttcttctg tttttgtctg ttggatatca gtcactactt
    17701 ccgctagttc tggcagttta gtagacaccg tatataaacg tattttggga ttatgtttga
    17761 gtaaggacaa atccaaattg tctttgacca tcttttccaa tggagcagaa gaaaccccgc
    17821 cttcaattag tgctgttttc gtcatttggc gtctttctcg atagaagcgt tcacgaaggt
    17881 cttcaactgc agccatttct ggtaacgcaa gcacttgtct tgtcgacagc tttttccata
    17941 aattcacagc ttttttcata tctccttgta aaatcaatgc gccgttcaac gcacctaccg
    18001 aggtacctgt gataatctca aagttgatat catgttcctt cagcgcctgc cacacaccaa
    18061 tctgataagc accgtgcgct cctcctctac ctaaaacaag tgctgtatga tagaccagct
    18121 ttttatttaa ttgtgtggtt ccttgatagc cgtttttatg aaaaatacgc aaccattctt
    18181 ctgatagagg agattgaaat aaaaaacaac agttttcttt aataaaaaaa cgagcgcaag
    18241 cttccatata ccgaaaaaat tttttccatt caaaagtttc accttcaatc aacagattat
    18301 taaaaataac atctcctcta cttttagcaa tagtaaaata gacggtatat tttttcgata
    18361 ctgctttaaa agcacattgc ttttttgcag aacgccaaaa agcgtctcta ctttcttgat
    18421 gactttgaaa aaatggaagg ggttgttttg cttgctggat aggcgaatac agcgacattt
    18481 tctgtgaaaa gtaaactttt tttatctcca tcgcgctctc ctttttcttc aagtatacca
    18541 gtacgtcata aaaaggtcat gagtctcgta ttcgactttg tagattccat aagaaaacaa
    18601 ttcataaaac ctcctttaaa taaaaaaata caaaaatcac aaaaattaat tgaaaatgag
    18661 agtgttttca atgaaaacgt gattttgtaa tggaatttta aaataaaaga aacaagcttt
    18721 tttaatgcaa atgttgtaaa ataaaagaga ttaagaggaa ataagaggag gaacatgatg
    18781 actgagtcag tttatgtaca tattgatacc acaagtaatg ctgttttaac aaaagggctg
    18841 actgctactg actttgcaaa cagtatcgtg cattttcctc aaaacttgct cttgcttgat
    18901 ccatctgctt ctgctggaga atatgaatcc catacagggc tgaaagtaat ccgtggtaca
    18961 gaaaatattc agcgtttctt ttcctcttct cgtaacaggc gttgattcga tgaattgaag
    19021 tggatcgact ttactgattt aacgatgctg aaagaattga cacctttaga aatttcagaa
    19081 ttattatatt ttggtcatat gaaaacacat cttcattcgc cgttttttta taaactacag
    19141 aataattttg tatacttcga tcttaatgat cagctgaatc ggatttatta tcgctattta
    19201 gatgaatttt ataggatttt tgctagtaaa ttaagttcga tcttgtcaga taagatcaat
    19261 gtaaaaaaat cttttttcaa aaaagaaaca cctgttgaaa aaatcagtgt ggaattattg
    19321 aaagaaatgc gtaatttgat gcaggaaggg atcatttttt cttttgatca agttggtttg
    19381 atcgatggta aatatcgtat cccaatctat gtgattgaag attatatttg gaaattaaaa
    19441 ggcacccggt atcgagaaga ggaaatcatc gctactttaa tctactctgt caccgatcga
    19501 cactggcaat tggtagaaga tgccgatctt tcttatctgc cattgaagta gatctatttc
    19561 ttatcaaaaa ataaaaagga aacaagaaac taggacatga ttctgtctca agtttcttgc
    19621 ttctttttta tttacagctc ctgtcgttta tctggcacaa aaatcgaagg agtcgtttgt
    19681 tggatcccta ataaaacgat gatcgttaca atagctgtac tcaacccgct ggcaccattc
    19741 attacgaaag aaaagagcca aggattcatc ccccatagtg catacgctcc ccagaaaacc
    19801 actcctgcaa taaaatgcca aaaatatctg gaaagtgcgc caatcaatgc accaaacacg
    19861 atgctttttc taatttgttt cgtattatcg tctacatagc ctttttggac atttgaagcc
    19921 atgagccctg ctgcgcctgc aaaagtatag gcaatcgggt actcaatcaa cacttgaacg
    19981 acacttaaat aataaacttg tcccaacgga aaatgtagga tgccccaaat gaaacctgcc
    20041 agcatccctg cctttgtccc tcttcttaac gcaaaaagag tcaatggaat ctgccctaat
    20101 gaaatagaaa aactgctgcc gatctggatc ggaatcaacg acaacaccat cgctaacgct
    20161 gcaacaatcg ttccttctac ccaaactcta ttttttccca taaaaaaact tcctcctttg
    20221 aataaaaaag tcacaaagga ggaagcagct ttcgaaaata gtcaaacagc tgtttattca
    20281 caattcctac gctcgcataa ccgatacagg tacgaagggt ttagaaatag attcaatctc
    20341 agcccaattg gactcccctt tgtgagttta tttattattt attttttgtt tcaaacaatg
    20401 gactaactgg tttgttttca tggatacgtt tgattgcatc tcccattagt ccgccaacac
    20461 ttacttcatc gattttgtcg attttacgat cttctggaag atagatcgag tctgtaacaa
    20521 ctagacgctc gatagctgaa tcttctattc tttgtaaagc agggccggat aatactggat
    20581 gtgtacatga agcatacaca ctagttgcac cagcttcttt caaagcattg gcagccaatg
    20641 tgatcgtacc tgctgtatcg atcatatcat cgatcagtac acaagttttg cctttgactt
    20701 gtccaatgat attcattact tctgcaacat ttgcttttgg acggcgtttg tctatgatcg
    20761 caatcggaga tttcaaaaat tccgctaatt tacgtgcccg tgtgacacca ccatggtctg
    20821 gtgaaacaac gacaacatca tcgcctttga ttcctttttc taagaaataa ttggcaataa
    20881 gtggcgcacc catcaaatga tcgactggaa tatcaaagaa accttggatt tggactgcat
    20941 gaaggtccaa agtcagcatt cttgttgcac cagctttttg aatcatgttt gctactaatt
    21001 tcgctgtgat tggttcccgt gcacgtgctt tacggtcttg acgcgcatag ccataataag
    21061 gcatgactac gttgattgtt tttgcacttg ctcgtttcag tgcgtcaatc atgatcagca
    21121 actccatcaa gttatcattg actgggctgc tcgttgattg aataacatag acatgcgatc
    21181 cacgaatact ttcttcaatg tttacttgaa tttctccatc actaaattga gtaacagatg
    21241 attttcctaa ctcgacgccg actgctgctg caattttctc tgctaatggg cgattggaat
    21301 ttaacgcaaa aatttttaat cttgggtcaa agtaatgttt tgacatgggg acctccactt
    21361 tctttttcac aactctagaa ttctacctaa taaatagtag tcatattttt taaataaatc
    21421 aagtgatttt gcaaaagtct tagtttagat aagggagttt ttttgcatat ccttctttat
    21481 tgacttgtct tgctctcgca attgccagcg catattctgg aatattgtct gtaattgttg
    21541 acccggcagc aatcgaactg tttttagcta cctcgacagg tccgatgata ttcgtgctag
    21601 aaccaataaa gctgtggtcg ccgacagtcg tatgatgttt gttctttcca tcatagttga
    21661 cgaagacaac accacatccg acattgattt cttctcccag cgtagcatca ccaacataag
    21721 tcaagtgacc gactttagtt cctttgccga tttgagcatt tttcacttcc acaaagtttc
    21781 cgatatggac gttttcgcca atttcagctt ttggtcgtaa atgtgcataa ggtcccacat
    21841 ctgcatgttt cttgacatgg ctgctttcaa tgactgagtt ttcaatgacg acgtgatcct
    21901 caatcctact gtctacgatt tttgaatgtg acccgatgac acaatcagaa ccaatgactg
    21961 tatttccttg gatttgtaca cctgcttcga tcaatgtgtc tggaccgatt tctacgcctg
    22021 catcgatgta tgtcgttgct gaatcgacaa agcttacgcc attgaccata tgcatctggt
    22081 taattcgctt tctcatgatc tcatttgctt tagctaacgc gatccgatca ttgactccca
    22141 ttgattcttc aaaatcatcc gtttgataag cagcaactgt atggccttct tcttttaaga
    22201 tttcgatgat atctgttaga tagtattcac cttgagcatt gtttgttcca actttgctta
    22261 acgcatcgaa caaagcttcg ttgtcaaaac agtaagttcc tgtattgatt tcttgtacaa
    22321 gagcttcttc tggtgttgca tctttttgtt ccacgatttt ttctacgatc ccaatatggt
    22381 cacggatgat tcgtccatat cctgttgggt tttctgcttg tgccgtcaag atcgttgcac
    22441 ttgcattttt tccttggtga tattcaaaaa gattgttcag cgtttcagta gtcaataatg
    22501 gtgtatcgcc actgataact aacgttgttc ctttttttcc ttccaagaaa gaagcagctt
    22561 gcaaaacagc atgtcctgtt cccaattgtt ctgcttgtaa cgcatattta ctgcgttctc
    22621 ctagttgtgc tttaacttgt tctgctccgt gtccaacgat cgtaatcact tcatctggtt
    22681 tcgtttcact gacccgatta atgatatgtt ctaccatagg tttaccagaa acgggatgta
    22741 aaactttata taattttgac ttcatgcggg tacctttccc tgccgccaaa ataattgcat
    22801 aacgtgcgtc caactgcgtc actcctaaat atagtttgct gatttcagtt tatcccagta
    22861 tacccatttt ttcaacaaaa caagtgattt tcttttatcc cagcgttttc tggataaaaa
    22921 aatagataga ccataacggg cctatctatt tggtaaccgg tgctctcctg ccaactcctc
    22981 ggtcacaagt cacactactc ataaaacatg aaaagctgtt ttttgaaatc gtttcttgtg
    23041 acttagagtt aaacggtagg cttacaacct cttcattaaa cgtttttcaa tcagctgacc
    23101 ttcggtatta gctcaataaa cggcaaaata tgaggagcaa tttttccgtt tattgagcta
    23161 atactcagga caggacgctg atttcacaac ctctagataa acggtgttca aaagccagtt
    23221 tctcggtatt aagccaaatt tatgaaaaaa tgtggaaagc cattttcaca aaatttttct
    23281 tactactcga aactaagcgg ctttttcaca acctcttatt ctgaaaaata atttcctggc
    23341 acaaccgtaa tatttttcgt ttgagtatct acatctttga cataaagcag tgacgtataa
    23401 tcatcgatgg cgcgatgacc gttgaatttt gattcggcaa atacagtgat gccaacaagt
    23461 tctgattcga attcttcaat caagctcttc attccgttga ctgttccgcc gcctttcatg
    23521 aaatcatcca ctacaaggac acgtgaacca cgtttcaaac ttctcttaga cagttccatc
    23581 ttttcaattc tttcagaaga tccagacaca tagttgacac ttactgttga tccttctgtt
    23641 atttttgagt cccggcgtac aataacgaat ggcgcattca gataatagga cacagcttgg
    23701 gcaataggta cgccttttgt ggctacagtc attacagcat cgattttttc accaaggtat
    23761 ttagaagcaa tgattcttcc aacttgacgc agcagttcag gttctcctaa caaatcagag
    23821 agataaacat atcctcctgg aagcagacgg tcttgttctg atagacgttc tgctagagat
    23881 tcaatatatt ttttcgcctc ttcatatgaa atttcaggga tgaacaatac accacctgca
    23941 gcacccggga ttgtttccaa ggttccatac cctcgttctt taaatgtttt cttgatgatt
    24001 cccaaatctt cactgataga agatttagca gattcataac gttcggcaaa gcttgttaag
    24061 gaaatcaatt catgcgggtg attcaataaa tactgtgtca tgtcaactag acgttcactg
    24121 cgacgtactt tcacctattc caactccttt taagaaattt tatcgtttta cattatataa
    24181 gtttttcttc aaaaattcga gcattttatg aaaaaataag aaaatagttc ggtatttatt
    24241 aaaataaaaa gaagcctata aaaaattttt cgtttttata aggcttcttt tctttataat
    24301 ttagttattt tttcttctga aggcaaccaa tattcgtttg atgatattta caatcaggaa
    24361 caaagcaata aagactaatg tgattgttgc tcctggaggt gtatctagat aaaacgagct
    24421 agttagtcct gacagcatcc ctagaaatcc tacacaaaca ctgatagcaa tcacgacatt
    24481 gaagcttttg cctaatcgca taccgattgc tgcgggaaga accatgattg cggatatcaa
    24541 aagtgcaccg gcaatcggga tcatcacagc aatagccaca ccagtaatca cattgaacat
    24601 catcgacatc aaatgaacgg gtagcccatc cacgtgggcg gtatcttcat caaaagtcag
    24661 aacatacatc ggccgtttga ataaaaagaa caatataccg atggcaacga ataagattcc
    24721 taaaaagatg acttgctccc atgtaatggt cacgatcgaa ccaaacagat aggattggat
    24781 acttgttgct gaattccctc cgctcaaatt catcaatact aacgctagcg ccaaaccacc
    24841 agccattaaa atggcaatgg agatttctga ataggttcgg tagatcgttc gcagatactc
    24901 caatatcaca gctgctaata caacgactag catggtcgta agtgtaggat taaggttcaa
    24961 aaagaatcct aacgctaccc ctgctaaaga gacatgagaa agcgtatcag ccatcagcga
    25021 ttgtcttctg atcactaaaa acacacctag catcggggcg attccggcaa tgaagatagc
    25081 ggcaagaaac gctcttctca tgaattcata tgaaagcaac gccattgaga atcctccttt
    25141 ctcaccagtc ggatctgtct gtccgcatac tgtttgatat cttcatgatc atgggtgatc
    25201 attagaactg atttcccatg gacatgcgca ctatgtcgga gaagacgata aaattcattt
    25261 ctggattctt catccatacc tgttgttggt tcatcaagaa caaataagtc agggtcagta
    25321 gcaaaaatac gcgctaaact gattcgctgc ttttgtccgc cagacaattc acctattcgc
    25381 cgatttcgca tatcccacat ccctacagct tctaaagcac gctgtacatg ttgatgatct
    25441 ttttcagaga gacgcttgaa ccagcggcct cttggatatc ttcctgaacg gactaattcg
    25501 atcaccgtac taggaaaacc tgcattgaaa gatgcaactt gttgaggaat ataaccgata
    25561 cttaattttt ttccttcttg atttttctca gcaatcttta tcattccttt tgaaggtttc
    25621 agtaatccta acgatgcttt caccaaggtt gacttggctg ctccattttc tccagttaaa
    25681 atgacaaatt cacccgcatc tacatgataa gagacatctt ctagtacagg ttcatcatca
    25741 tagtaaaagg tcaaatcaga tatttcgata taacgcacct tacaacctcc attctaaaac
    25801 ttaattgata ctttctttta aagctttcag atttttttcc atgacagaaa tataattttc
    25861 gcctgctttg atttgttcat tagtcaagct ttctaaagga ttcaacactt ctagcttcac
    25921 tcctgtttca ttcgacaatg tctcagctac cttcgaagag gcattttctt caaaataaat
    25981 cactttgatg ttgtttttct tcacaaattc ttttagctca gctaagcgac ttggtgtggg
    26041 ttcctgatct ggtgaaacgc ccgaaatcgc tacttgtttt aagtcgtatt gattcgctaa
    26101 ataagcaaaa gcagcatgct gagtgacaaa tgtgcggttt gttgcatctt ttagctcatc
    26161 ggagtatttt tgatccagtg cttcaagctt tttgatataa gcatcggcat tcttcgtaaa
    26221 agtttccttt ttatctggat atttttcgcc taattcttca gcaatcgttc tgacttcttt
    26281 tatagctaat accggatcga gccaaacatg tggatcgtat tcatgtgaat gaccatgccc
    26341 ttcttctgat tcttcatgtt cgtctgcttc ttgcgattct aaacggtcga ttcctttagc
    26401 tgcttcgatc atcaaggtct gtttgctatc cactgaatcc tttaacgaat ccacaaaaaa
    26461 ttccatgtct gaactattat acacgaaaac atctgcatcg gaaatttctg ctctttcttt
    26521 tgcacttggt tcgaaatcat gcggctccgt tccagcaggg atcaaaagtt tcacgtttcc
    26581 ttcatcccct acgatctctt ttgtaaaatc atacatcgga taaaaagtcg taacgatcgt
    26641 caaatcctct tttttgtctg cttccttgtt cgtatttcca catgctgcaa acagtaaagc
    26701 acctatcatt accgtagctg tcaaaagata ttttttcatt tatatgctcc tttcaatgaa
    26761 caaatcggaa ttttacgatt tattaacgtg aactaatgta tcaaaataaa aaaacacagt
    26821 caatagaaat ccatcaattt ctgatttaag catgtatatt cttgattaaa tcgtaaaatg
    26881 gatgagttgc tgctcatatc tagccatttc actacaataa aaaactgaag aataccattt
    26941 taggattcct cagtttttca tcacctattt tacttcttat attttattgc agggttcgta
    27001 ctaaataaac ttcctcacaa aaacctttta acccattata tactctctgc gctcttgaat
    27061 aatgttgaca caaggcaaaa acggttggtc cgcttccact catcaatgca gcatctgctc
    27121 cgtacttgat catacgctct ttgatttgat ggatgactgg atgccgcttt cccgttatat
    27181 cttctaaact gtttcccatt tttttgatca tctgctgata atcttgctct cggatggctt
    27241 cagcaagtcc gtggatgtct tgatgttgga tggcttgcgt atctacttct ctaaacactg
    27301 tacgtgtaga gacactcatt cttggtttga ccaacacaac ccagcactgt ggcatagaag
    27361 gtaatacttg gatttcttcc cctttgccac cgacaaatgc cgtacttccg tagacacagt
    27421 aaggaacatc tgtaccgact tccaaaccga tttctgccag ttcttctaac gaacagccca
    27481 gattccataa ttggttgatc cctcttagag cagctgcaca gtctgtactt cctccgccta
    27541 atccagctgc tactggaata ttcttcttaa caaagatctt gacaccggtt ttgatattgt
    27601 atttattttt tagcaataaa gcagcttgat aaacattgtt ccgttcatct accggtaaaa
    27661 atgctttatt tgtttctaca atgattttgt cttctgttat ttcttcaaaa aatagatgat
    27721 ctgccaagtc tacgctcgac atcaccattt ccaactcatg atacccatct tgccgtctat
    27781 ataatacatc cagtcctaaa tttatcttgg ctggcgcttt ttcaatcaat tccatcctct
    27841 cacccattcg aaaagtctaa gtcgcaagta ttctatcata gggaaaaata aaagtatagc
    27901 ttatgtctgc ctagtggttg attttgtcat cgtttcaaca gctttcacag aattttctct
    27961 tcataatcca aacaagttct agtgattctt ataattctgt gagcacttct tctgccaaac
    28021 tcggaacatt tcctttttct tttcctatca gactgagtag ctgcatggta gcaaatccga
    28081 aattacttcc tttcctataa gcaaaataac gtccttccca atgatcgaca tattttaatt
    28141 tcttttcata agcagactgg aaggaataga aaaaattacc ataattatat acgatatgca
    28201 tgactttttc ttgaaagaaa gaaaaatctg tttctccaac attagctaat ggagctaagc
    28261 ctagattggc ttccgagtag cccattttct cgtatacaat aaacagattg gctaacagat
    28321 agtcagctaa ttcctcagat tctttaggta agacccgcaa caaatcatat cccgcctgct
    28381 tcttcgtcat cggctgagcg gtgatgaagc caacgacttt ttgatcttta cggacaatac
    28441 cgacaccgct agataataca tatgatttat caaatcgtcc cacagagaag tatttttctc
    28501 gtgcaccttg aagccatttt tgtgaaactt catttacagc ttgatgtaat tcttccggca
    28561 gttgatcgta ccaagtaaaa tggtatcctt ctttcatcaa acgttgatat tcaagttgct
    28621 gactcagccg agacaagtct tctgttgcta gatcaacaat ccctgcttca cctactttcg
    28681 tgaagtgaaa acccaattca tgaagcatca ctacgtagct ttctgatatc ttataaaacg
    28741 caagctgata gcctaaacaa tctgcttgtt tcataaactg attcgttgct gcccgcatca
    28801 ttttaggatt gccaactgga tctcctaaaa caaagcattt gtttcctttg atctgataac
    28861 caaaaacgac ttgatcttgg tcgtcttctt ggtaataaaa ataactgtaa cctgacaagc
    28921 gaagctgatg gcttgtagct gtgcctccgt atgtttccaa taaacggtca aaacggtctt
    28981 tttcgtactc actgccaagc ttcgtagtat cttcagctaa atattgataa agcgccacta
    29041 aagctagcaa tgagatgccc aaaccaataa gccctgagaa ccacacatca tctgaaggaa
    29101 acaaaataaa atgatttcct gttattcgac cgactaaaaa gcgtccgctg tgatagcctg
    29161 caattgcgta aacgataaac aaaaagccga acaagctagc atcaaagatt atagctcccc
    29221 aagaataaac gaattttgtt cgataaaatt cttttctgga taaccacaca gctaacagaa
    29281 tcaagacata taccaatatc aactgccaag ataacgtacg tgtgattgta tttaaaatac
    29341 caaaagatag taaagcaatc gtaggcagaa atgccttttt gacttttact gaaagtgctc
    29401 tagcaaggcc gagtaataaa aagccaacca atagattcac tgtctgatct aaaaaattga
    29461 atgagaacgg caataatact tgaaatagcc gactgattgc agacagatta gtgattgttg
    29521 atagaagtac catcatgatc cccgcaaaat agacagcact tacgagcacg atatgcgcaa
    29581 ccttttggaa aaacagtcgc ggaatgttat ctaaaaatcg attgattttg actcctgtat
    29641 gtgtgatgaa caatagaagt cccgtcataa aaggtagaac ataataaaac aaccgataat
    29701 ataatagcca aactactgct acatcttgcc gaacagacaa ttgactcatt cctaagatca
    29761 tcaaaacgtc aaaagtcccc attccacctg ggaccatcgt cagcattcct atcaatgtag
    29821 caatcatgaa cataggatag atcgaaagca tcgaaatatc tattctcatc aacttgccga
    29881 tgatcaaaaa taccgcaagg gctcccgtcc attgtccaaa ggatgccagt agcagaccaa
    29941 gcaagccttt tggataaaac tcgaagaaca atgtctttct ctttagataa gcaaaaaaga
    30001 aaatgactgg tgctaaaaaa ctaccgccga aaagccagat ccaatagctt cgaaaaatac
    30061 tttctgactg aataaaaaag acatcaataa atgtaagaaa cgaccataca gacaaaccag
    30121 tcagcataaa tagtgcgact tttgatacag tggctaaaac catttttcta tttgtctgct
    30181 ttccataaaa actagcccgc agacttgctc ctaccacacc accaaaaccg gcgagattgt
    30241 tgatcgtgtt tgttacccac gcagcaagaa gaaactcttt tctttccatt tttggttttc
    30301 cttgtttttc taagactctg atcgtcaccc aatcatacaa caacatcgga gtgacaccca
    30361 gcagtccagc cagaatcatg agcaggatcg tcatgcggct ttgttgtccc atcctttgcc
    30421 aaatatcttg ccaactcatg ccattagcaa tatttgcgac ttgatttgcc acaaagacta
    30481 acacggagcc gaaaaaaatc agttttaaaa tcagactatg ttcctttatc cattgcagca
    30541 tcaagtatcg tttatttttc acattttccc attcctaact tgtatacttt tgtgtcatac
    30601 cccaatgtta tcttatttat tttaattttt ccactttaga cctgttcttt gcttgcgatg
    30661 atagaaaaag aaagatatcc taatgctaaa ggagaggata acatgaaagt aggatttatc
    30721 ggcacaggga atatggcaca agctattatt gatggtttat taaaaaaagg aacagtctcg
    30781 aaagaagagg tgttcgtttc cagtggacat tttgaaaatg cacaaaaatt tgctgctgaa
    30841 actggcgtcc atgcatgcaa aagtaatcag gaagttgcat cacaatcaga tatcgttctt
    30901 ttagccgtga aaccaaaaat cattgaaaaa gtattggaag aactaagaga atatagccag
    30961 aaagtcttat tcatttctat tgcttcagga aaatcattaa tagatttaga aacagcgctt
    31021 gaagatcctc aggcctccat cgtacgtgtg atgccgaatg tcaatgtcag cgtaggagca
    31081 ggcgtttctg cgatttgcaa aaatgaagct gccacggagg aacagcttca gcaagctacg
    31141 atgatttttg aagcaatcgg cagcgtctat cctttagcag aatcagattt cagtacattc
    31201 attggccttg ctggcagctc acccgcattc gtttacatgc tgatcgatgc gatgggacgg
    31261 actggtgtac tgaatggttt gtccaaacag acagccactg agattgctgc aaaagcaatt
    31321 atgggttctt gtcaaaaatt cctttcaagt gataaaaatc catgggaact tgtagaccaa
    31381 gtttcttctc ctggcggtac aactgttgca ggtgtagttg ccttagaaga agcaggattc
    31441 atcccagcaa tcatcaaagg aatcaatacg acgatcgaga aagatcttga gatgttgaat
    31501 aaaaaataat gatagcaatg ttttttcgtg ccttacaaaa aaagaagagc tgtggctttt
    31561 gccgcggctc ttcttttgta ttttttatta tttagcataa tcagttgcac gagtttcccg
    31621 aataacgatt actttgatgt gtccaggata atccagttct tcttcgattc gtttacgaat
    31681 atcacgtact aaacgaacag cgtctaaatc cgagatttct tctggcttga ccattacccg
    31741 aacttcgcgt ccagcttgaa ctgcgtaact tgattctaca ccggcgaagc tgttcgaaat
    31801 attttccaga cttcttaaac gacggatgta gttttctaat gattcacttc gcgcaccagg
    31861 tcttgctgct gagagcgcat ctgctgcagc tacaaggaca gagataacag aagtagcttc
    31921 cacatctcca tgatgagaag cgatcgcgtt gattactact gggttttctt tgtacttggc
    31981 agctaattcc gcaccgatct caacgtgaga tccttcaatc tcatggtcaa gtgctttccc
    32041 aatatcatgg agcaacccgg cacgttttgc taattggata tcttcgccaa gctctgctgc
    32101 taagatacca gctaattttg caacctcgac cgaatgcttc aagacgtttt gtccataact
    32161 agttctgaat cgcagacgtc cgatgatctt gattagatct ggatgaagtg tatgagcacc
    32221 gacttcaaac gctgcctctt ccccgtattc gcgaatacgt tcatccattt ctttacgaga
    32281 tttctcgacc atttcttcga tacgcgctgg atggattcgt ccatcttgta tcagtttttc
    32341 caaggtcatc cgtgcgattt ctcttcggat tggatcgaat ccggaaagaa caactgcttc
    32401 cggtgtatca tcgatgatta aatcgatacc agtcagcgtc tcaagcgttc gaatattacg
    32461 accttctcgt ccaatgatac gacctttcat ttcatcattc ggaagggtaa cgactgaaac
    32521 agtcgtttct gaaacttgat ctgctgcgca gcgttgaatc gctaatgata ataagttttt
    32581 cgctttgcga tctgcttctt ctttggcacg ctgttcagat tcttttacca ttagagttaa
    32641 ctcatgattc agctcttctt cagtcgattt catgatgaca tctcgtgcgt cctcttttga
    32701 taaaccagca attttttcta gttctttttg ttgatcttca atcatttttt caacgtcttt
    32761 ttctcgctct tcaattaatt gctgttttga actaagtgct tcttctttac tttcaagtga
    32821 gttctcgcgt ttttctaacg agtcatcttt acgattcagc gtttgttcgc gttgaatcaa
    32881 acgattctct tgagatttta attctaactt actttctctc agctcacttt caacttcaga
    32941 acggtatttc tgattttctt ccttagcttc taacaatgct tcttttttta gagtttctgc
    33001 ttcttttttt gcactatcga tgattccagc agctgatgat tgtgcaccag ctatttcttt
    33061 ctcgtgacgt gactttgcga ccataaaccc taaaccaaga ccgacaatta aaccgatgat
    33121 agcgaggaga atgttgaata ccataaaatc cacctccata ctatcttttt catttttatc
    33181 atctatacag cttctatgat aaattattct aaactcgtca atatgcatct tcgcatatat
    33241 gtgtacaaaa aacatgcaca actttattct aatgtttgta ccccgtagtg tcaactttaa
    33301 ataaacaaag aaacggcttt ctacttattc attaaacgat ggtttttaaa ataaatgaca
    33361 ctagtttcat ataggtaaac ctttaaattg agaaactttt ctattaaatt attctttgta
    33421 gatgaacaga tatttttatt ttatgtaccc actcgtataa ttaaataact cacgatttct
    33481 ttattcaata aaaaaacggc aggtcaagga gagtacctac tcttcagacc agccgttttt
    33541 gtttatcata atttttattc ttctactaaa ggcaattctt cttgtccttt ttcatcttct
    33601 gggacagcga cttcttcacc aataccataa gctgctctaa ctagagcaga aacttctgcc
    33661 atcatttcag gatgattagc catatagatt ttagcgtttt cccgtccttg accaatccga
    33721 tcttctttat aagaatacca agcaccgctt ttatcaacaa tgtctttttc tactgccatg
    33781 tctagaagtt ctccttcttg tgagatccct aaaccataca tgacatctac ttcagcgatt
    33841 ttgaaaggag gtgctacttt gttttttacg actttgatct ttgtacggtt accaacaata
    33901 tctgtacctt gtttcaattg ttctgcacga cgtacttcca aacggatcgt tgcgtagaat
    33961 ttcaatgcgc gtcctccagg agtgatttct ggattcccaa acatcacgcc tactttttca
    34021 cggatttggt tgatgaaaat agcgattgtt tttgttttgt tgatcgaacc agagagttta
    34081 cgcaatgctt gagacatcaa acgtgcttgt aacccgacat gtgagtcacc catttcgccg
    34141 tcgatttctg ctcgtggaac taaagcagca actgaatcga ctactacgat atctacagcc
    34201 ccacttgata ctaaagcatc agcgatctct agaccttgtt ctcctgtgtc aggctgtgaa
    34261 agaagtagtt catcgatatt cacacctaat ttttgtgcat attgcggatc taacgcatgc
    34321 tcagcatcaa tgaaagcggc cgttccgcca tttttttgta cttctgcaat agcgtgtagt
    34381 gcaactgttg ttttacctga actttcaggt ccatatactt cgatgatacg tccacggggg
    34441 taaccgccga cacctaaagc gacatctaac gcaagagagc cacttggaat tgtagagatt
    34501 tgttggtcga ttttttcgcc caatttcata atagagcctt taccatagct cttttcaatt
    34561 tttttcagtg cagcatctaa ggctgcttta cgatcatctg ccaatacgat aatcctcctt
    34621 atatttacaa atcagagcaa catttcatta tccttatcat atctttttta gtgaaaaaaa
    34681 gcaagaaaaa accgaacaaa tattcgcatt tatcattttc ttaaaagctg ttttctcaaa
    34741 agatcaaatc cttgcataac cgcattatat ctgatagcag cacgatctct ggcaaagtga
    34801 tatttttccg aaaccacgcc gtctttagat gaaatagcga tccatactgt tcctgcagat
    34861 tgaccttcta acgtgtccgg tccagccact ccggtgaaag ctacactgta atctgttctt
    34921 gctatctcac gtgcttgaat ggccatctgc tccacacatt ctttgctgac tgttccatat
    34981 tctgctaata gctccggatc aatctccaga aaaccagctt ttgtttggag cgagtaggta
    35041 acgaatccgc ctggaaatac ttcagataca ccagcaatgt cgcctaatgc ggcttggaag
    35101 gcgccggctg tcaaactttc cgctgctgtt acagtctgct tgttttcttt cagtagttcc
    35161 actacgactt ttgctagcga attatcatcg ccgtatccat aaaaatattc ccctactcgt
    35221 tcttggattt tttcttctaa tgctaaaaga gcttgattcc ctgcctgaac atcatttgtt
    35281 ttcactgtca gtcgtaatgt cacttcattt ggtttggcat atggtgcgat cgttggattg
    35341 atctgtgtct caatcagatc ttttagctca gtgaccagtc gcgattcacc aatcccataa
    35401 aagcgcaaaa ctttagaaat tagtttttct tcagaaggaa aacgttcctc tagtaaagga
    35461 cgtacttgtt ccacaaacat tggtttcaac tcattaggcg gaccgggaag aaggatatac
    35521 gcatgtgtat ctgtttgata aaaagtccct aaagcaagcc ctgtacgatt tggtagagga
    35581 accccgcctt cgataatctg ggactgttgc agattgtttt tggtcatttt tcgatgggtt
    35641 gtttcaaaat atgccaataa ttttttgtat ccttccgtat tttgtatgag agacttaccg
    35701 aggtgagccg cagttgcttc ttttgtcagg tcatcttctg tcggtcctaa tccgccacat
    35761 aagagtatga gttcacttct tgtttcagct agagcaatca actcttctaa acgctgctga
    35821 ttatctccta caactgtttg ataataaact tcgataccta gatcagctag ctgttctgac
    35881 aagaaagtgg cattcgtatt cacgacttgt cctagtaaaa gttctgttcc gacagctatt
    35941 atttctgctt tcattacttc ccgctccctt atatagtata cgctgtattt ttatattttt
    36001 ttatttaaaa agagggcaag gagctgtgac aaaaatcttg tcacagctcc ttgttccaaa
    36061 taaacagtgt tctcctgcca aaacctcggt cataagtcac aatatacata aaacatgaaa
    36121 agctgttttt tgaaattatc ccttgtgact cagagttaga cggcaggctc acaacctctc
    36181 cactaaacga tacttacatt gatcctttga acacaccgct gtttttgatg aagtaatcca
    36241 cacctgaata gatagtaaag atcagacaag cataaagcat gatttgatct actggaatac
    36301 cgatcaggct gaatgggata ttattgataa acagcaagat aatagcaacc atttgtgtag
    36361 ccgtcttgac ttttcccggc caagctgcag ccatgacttc tccatgttcg acaagtagta
    36421 aacgcaagcc tgtgacggct aattcacgac agacaatgat cgctactacc caaccagggg
    36481 cctttccttg gtcaaccaac atgataaaag cagtcataac tagcatttta tctgccaacg
    36541 ggtccgcaaa cttgccaaaa ttagtgacaa ggcctcgcgc acgggcaatt tttccatcta
    36601 accagtctgt gatactagct aaagcaaaaa taactgcacc tactagctgt gtgacagcca
    36661 gtgtggtgtc tcctacgtta aattgtcccc aatctaaagg taaacctaca accaaaatga
    36721 aaatcgggat catacaaatt ctaagtacag ttaatttatt tggtaaattc acactatcga
    36781 ctcctttctt ccctattcta cgtgactatc agcgaagttt ccaccgatat tatttacttt
    36841 tccaaatgaa tttattcaaa ggagtatttt tattgaaatt gatctttttg aattaacgaa
    36901 gaaggaccgc gacacagatt tgtcacgatc cttctttttt atacctacaa gtaaacagat
    36961 gcgtaaacta atttatacga tcattctgcg tattgaagag taaggttcac attttttgga
    37021 ctctgattgt tttcccctgg attaactggt acggattctc cattcgcttt gatcgttgca
    37081 ttgcttgctg caccaatcgt gatggtcgca tttgttgctg tttccggtaa cacggtactc
    37141 tgtgtttcat ttgctgccag tgtcccttga taaacatagg cattgtctac tagcacacct
    37201 aaccatacac gattgacagc agtaaattca agggttacag gcttggtagc gtctgtgata
    37261 tttactgcga tggcactgcc agaatcttct cctgtagcta ttgccatttt cttttcttca
    37321 cttgattttg ttgttgattc tactgtagaa gactctttgg tctgcgtgct ttgctctgag
    37381 gaagaggact cctcgactga tccttctact ttaagcgaag aagctgtgtc tccgattatt
    37441 ggatcagctt gacgatcttg ccaagtcata tagcccacga caacgacaat cgccaaagca
    37501 accaatccta ataaaatgac aggaagactt gtccaaaagc gacttggatg cgattcttcc
    37561 tgatgcattg cttttctaga agtacttact gtttcaggct gcggacgttc agggaccgat
    37621 cctgtcaatt tgcttttccc atcaaaaaca tcgactaaat ggtctccatc ttctcctaca
    37681 gcttcagcgt attgccgaat aaatgcccgc acgtaaaatt taccaggcaa ttggtcaaaa
    37741 tcatcattgt cgattgcttc caaataacgt ttttgaattt ttgtaatctg ctgtaattca
    37801 tctaaagata tttttttatt caggcgtgct tgccgtaatt tttctccgat ggtttcaaaa
    37861 gccactcgtt catcttctcc agtctgtttg attctccgaa gcatctgctg ttgcaacgaa
    37921 agacgttatg tttgggtgct gtgacaaaag tcatgtcaca gctccttttt tccgaataaa
    37981 cattgttctc ctgccaactc ctcggtcaca agtcacaata tccaataaat ataagaagct
    38041 gttttttgaa cttgttcctt gtgactcgga gttaaacggc aggctcacga cctctttctt
    38101 aaacggtgtt caatcagctg ttcctcggta ttagctcaaa aaccggcaaa atataaggag
    38161 atatttttcc gtatgtttct ctaatactca ggtcaaaacg ctgatttcac aacctcttcc
    38221 ttaaacggtg ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacatta
    38281 agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt ttcacaacct
    38341 cttggtacac ggtgttccag aagtagcttc ttcggcaata agccgaaata tcacaaaaat
    38401 ttgagaagca attttcgtaa attccttctt atttcttgaa gctgaccact tctgtcacaa
    38461 cctctttgat tacttcgttt gattttattt taatataagt tgattaaaag agagcatgat
    38521 ttgttttatt ttctcactaa tttttccaac acaactaaga ataccataaa agctgatttt
    38581 ggaggaggtt cctactacaa gtctaaaacg aaatttaaga cttttcctca taaaatactg
    38641 gtttattttg gtctcatgta aaactcgctg aaggcctctt cacttataaa tgcttcccca
    38701 gctgccagta cgtctttcat ttggatcgaa tcgatgattt caggaagatc aaataaagtt
    38761 cgatcgccaa ataaactttg cgtaaattga ttggcaatat attcgatcga atttaatgat
    38821 tggaaatatt gtccaagcat tttcttcttc agcaaatcga gatttgtttc tgacacttcc
    38881 ggatcatcag cgaatccaag aatgatttct ttgacttttt ctgctgcttt ctctggttcg
    38941 tccgtatctc cgctgaagtc cgcaaagtga aattcacgat ccaagctgaa ttcaaaacca
    39001 aagctgtcat cgataattcc ttgattgtac atcgctagat agtttcgaga agtattgcct
    39061 aaaatcattt ggaacaacag attgattgct gttttatagc gtaacagttc tgttccttct
    39121 tgcggcaatg tgtctaatcc tttaatacca agtacaaatt tatctcgtgt aatggctgct
    39181 tccaatgcac tttgtttgat gatttctttt gtgttttcag ggaaataacg tacaatttct
    39241 tgctttggag ggaaattttt tgcttcttgg ttttcccgaa taagtttcat caatttctca
    39301 ggctccatct taccaactac aaataaaacc atattgcttg gctgataaaa cgtccgatag
    39361 cacgtataca agtcttgagc tgtgattttg tcgatacttt caaccgtgcc cgcaatatcg
    39421 atatgcaatg ggtgtgtagg atacaagtta tttagtatgc caaaaaacat ccgccagtta
    39481 gggtcgtctt catacatttg gatttcctga ccgatgattc ctttttcctt gtttacagtt
    39541 tcttctgtga agtaaggtgc ttgtacaaaa tcgatcagag tcgtcagatt cttttctacc
    39601 tgatccgtcg tagaaaacag atagctggtt ttcgtaaaac ttgtgaaagc attggcggaa
    39661 gctccttgtt tcccaaataa ttgaaagaca tcaccatctt ctttttcaaa caacttatgt
    39721 tctaaaaaat gggcaattcc atctggaact ttcacctttt ctttttcacc atagggaata
    39781 aattcgttat cgatcgaacc ataattcgta ctgaacaaac catatgtctt gtgatagtca
    39841 tttttaggca acaaatacac ggtcaaaccg tttggcagta cttcatgata aagtgtttca
    39901 ttgatttgct catattctgt tttattcatt tgcatttcct cctgctaaaa agaagattgc
    39961 ctgcaattcg atttgctcag ctacttgttg tatctctttg atcgttacgc tctccatacg
    40021 tttcaaccat tcttcatcag ataattttgt ttccggcagc cagctgtcca aaaaagaagc
    40081 ttcgattgcc gcttgcggag agtctagaga aagcagatat tggtttctca gcattgcttt
    40141 tgtctgagac agttccaact ctgttatttc accatttcgc aaactttcta attgctcgtg
    40201 aatcagatga agtacttgat ttcggttctt ttcatcgata ccggtttgga cactcatgaa
    40261 tcctcggaac gtatccacgc tactagaggc ataataagct agactttctt tctctctgac
    40321 attcatgaaa agtttagagt gaggaaaacc gccaaataaa ccgttaaaga ccattagcgc
    40381 aaaacgttct ggttcatcgt aatacacatt tgtctgataa gctaggttca gctttgcttg
    40441 aacgattggc tcacgtactt gtcgttcctt cacgatattc acttgaggtt gggtataaaa
    40501 cagatctgga taaactgttt cccgcgactc aaatggtaat tgcccaactg cttcagctgc
    40561 tttgtccgga tcaacatcgc cgacaacaaa aatgtcgatt tgatcatttg ccatcatttc
    40621 ttgatagtag gacgctagcg aactagcagt gatcttgtcc aaatcttcga ctacgcccaa
    40681 ccctgggatc ttttgttccg ggctgttttc aaaataaagt tcttgcaagg cataggaagc
    40741 aaaagtttgt ttatcttcct tcaaactttc caaatataga cgcagattgt tcttttccaa
    40801 gtcaaacgtc aactgatcaa actgatgatt tttaatattt ggataaaata aaacctcttt
    40861 taaaaaatct accgcttgag aaaaaaggtt tggatcattg acatattcac cattcacaaa
    40921 actgatccct gcgtttagcc aatgtaaatt tccttttttt ctaactgata atccaaagct
    40981 tgcgccataa agctctgcaa gttgcgcact taactttgtc tgatccgaat aattcaaact
    41041 atttgtttcc aacatgctgc tgagaagtga acgtttcgtg atgacttctt gttgcagacg
    41101 cgcagtaaaa cggataaaaa tccggatcgt tttgtattgt tttgttggta agacatgaag
    41161 attcacgcct tctgccaatt gatacaccat atttatatgc tccttttcac agttgattgc
    41221 ccgcttactc ttttaatggg tcaacctgcc ttttttactt attcctctgc ttgaaattta
    41281 taagttcagg caattcgccg ttttttctaa atgatgttac actttatagt atagcataga
    41341 tgttttttat caaaagaaac agacggctaa aagcgtatat tgttttttta ttttttattt
    41401 ttcgatatac taaagacaaa taaaagaagg gagggtcatt catgatcaaa gaattcaaag
    41461 agttcatcat gcgtggagat gtcttggatc ttgctgtcgg cgttgttatt ggtagtgcgt
    41521 ttacaagcat cgtaaaccaa gtggtgcaag gcttgatcac accactaata ggttggtttg
    41581 tcgcaatgat cacaggtact tctgatttag aaggagcact ttcaattctt gactggtcgc
    41641 cgacaaaagg cgtcacattt gcgtttggta acgttattag tgctatcatt acatttctga
    41701 tcactggttt tgtcttattc ttagttgtca aagcggcaaa ccgtgcgaag aatttacgct
    41761 ccaaagaaga gcaagtggaa gtggaagaaa ctcctactgc agaagattat cttagtgaca
    41821 tacgcgatct tcttgccaaa caagtggcag aagacaatac acatccagta gagcaaacaa
    41881 atctttccga tacttctaaa taataaagaa tctaaaaaaa agaaagccga ttttcagtgt
    41941 cggctttctt tttttagatt cttaacttgc tacttgttcc tttttcatgt cgatatgtgg
    42001 aatatcatct tccaaataca catctgagat tgccttgaat ccaaacgact cataaaaagc
    42061 ttgtaggtat tcttgtgcgc cgatcacgat tggtttttct ggaaaatttt tctggatcat
    42121 gttaatcgtt tcttgaacaa tctttcttcc taaaccatct ttacggttgt cctgatgaac
    42181 aaggacacgt ccaaaatgga tactctccgc ttcttcatat actcgcgtgt aggcaaggat
    42241 cttcccatca gtatcttgaa aaaacgtatg ccaagcttct ccatcaatgt catcaacttc
    42301 ttgataagga cactgttgtt ccacaacaaa aactgcgatt cgttctttga cgatcgcgta
    42361 aaactctgct gttgacaatt cttcaaattt ctttacctga tattccacta agcaaacgcc
    42421 ccctgctttt ttattcttag cataccgatt cgcacacaaa aaagcaaacg aaccttacga
    42481 atctcaggct cgtttgcttt ttaaactgct tcagttaaaa attcgatctc aactgtccga
    42541 gtaagtacat ctgaatagct gtaagatact cgttcaaaag aattttcttc aggatccaaa
    42601 tcaacaacga atactgaagg ataggtttct gttaaaacgc cgcggcgttc tgtttgacgc
    42661 tttcttcctg tctgagcaac caacatgatt gggctgccga tacgaccttc taaatctttc
    42721 ttgattgaag ctaaagttgt tggcatacag ttcacctcta ctatcaacta taccacatat
    42781 ttttaaaatt ttcaagttta ccataaaaaa aaaagaaatg caataaaaat aaataagaag
    42841 ctcattaaga tttttctaaa ttattcctca ttataaaatt tcaagtgttt ttttattttg
    42901 agttttactc tcccgtaagc gctcattact tgcttgtgat ccattgctaa acattcagcc
    42961 atgtctacaa aatcaacgcc ttgcatataa taatgaaaaa ttttagcttc tatttttgaa
    43021 aactggattt cgcaattttc gattgcttct tttaataaaa gatgctgaat tgctaaaggt
    43081 tcttgcaatg tgatctctgc taaaaagtct ggtccttctg tctctatttt ttggtcgatt
    43141 gagacttcat ctttttgatc acgacgcttc agcgcgttct gcttcctcaa cagagaatag
    43201 atttgattgc ttataacgct tttataataa cttccaaagc taacaccttt gcttttttcg
    43261 taagttctca atgcctgata taaagcgatc cttgcttctt gttcccaatc ctcagaaaca
    43321 taatttctta aataataggt gtttttcaaa cgtagtaaaa gtggaaaata tttttgatac
    43381 agggcagcaa atgcttcttc gtcaccatta cgtgcagcgc gcaataactc ctcagacata
    43441 aaataattcc tccataaatt cgtttatgga ggaatcttat catcaatgat tcatcgaact
    43501 gattagaacg atatgttatt tattttttct cattaaatcg tccaattttt gggacaattt
    43561 ttttaactgc tcttcattcc atggagaatt acgtcggaaa tcaataaaat gcatatcttt
    43621 attttcttgg gctattgttt tttccgtttt ttttatcgtc ttatataatt cataagcaga
    43681 agttcgtaat gctccttggg aaaagatcat ccactgttct gctaaatcgc ttgtcgccac
    43741 tgtcacttgt gtcaaacggt cattcagctg accagcgatt ctctcgatat aactgtcagc
    43801 cgtttcgtct tctttcgtaa aaacaacatc caactggtat ttcttataat tttgttggat
    43861 gcccggaact agttgggcat cgaagacaac gatgacttcc attccttcat atttcgcata
    43921 atttgataaa cgatgtaaaa gagcttctct tgcatccgcc attttgtcct gctttttaag
    43981 ctggacaagt tccggccacg aaccaatcat gttgtagccg tctacaatca acagctgttt
    44041 tttcatggtc tactccccct gacgttggga aaaagcctga tacataagca aaccagcagc
    44101 aacactggca tttaagctct ggacgtgccc ggtcatcggg atcgtcagca attcatccat
    44161 ttcttttttc aatcctggac tcattccttt gccttcatta ccaatgacta aagcaattga
    44221 tcctttggca ttccattttt ggaagtttgt gccggtcata tctgtgccaa atacccaaaa
    44281 tcctgtttct ttgagctgtt gcacagcttg taccaaattc gtcacgcgtg caacagggac
    44341 atgttcgact gccccagtgg aagtctttgt tacgataggt gtgattccaa ctgctcggtg
    44401 ttttggtatg atcacaccat cgacgccact tgcgtctgct gtccgtaaaa tcgacccaaa
    44461 attgtgtgga tcttcgatgc tatccaaaat caaaaagaag gggtcttctt ttttctcttt
    44521 tgttcgttca atcagttggt caagggataa atattcataa ggtgtaatcg caagaacgac
    44581 tccttgatgc acgccgtgat cgctcaaagt ttctaatttt tgtttaggaa cccatttgac
    44641 tggcacagct tgttcacgtg cgatttcttt cagttcttct actttttctc ctcgtgaatc
    44701 ttcttgaaga aagagtttat tccctcgccc ttgttgtagc gcttctactg cggcatggtg
    44761 cccaaagacg aaattatctt ctaatgcttc ttcattttga tcagaaactg actgatttcg
    44821 tccagtttgc tttttatttg aatatctatt ttgttttttg tcttcaaatc cacgtttcga
    44881 ttgatgtttg tttgttcttt ctttacccat ttttttcacc aactttctga atacaccagc
    44941 ggatcaattc ttccatccgc tcggtttgtt ttgtcaaatg cagatagccc atgagcgctt
    45001 cgaaccctgt tgcgacccga tacgtcgtaa catcagcatt ttttgcgctc gtatggcttt
    45061 tagcatttct tcctctgcga taataaagtt cttcttcttc tgtcaaaaga tgttcttcca
    45121 gcatttgctg aatcaaaaag gcttgtgcat tcgcagaaac ataattcgtt gccgcacgat
    45181 gcagcttatt gggttttgtt tctccttgat caatcaggtg gtcacgaaca tagatttcgt
    45241 aaatcgcatc tccaacataa gccaaggcta aaccgttcaa ttgtgtgtaa tctctcatgc
    45301 ttctcttctc catcgggttc cttgcggtgt atcttctaat aagatccctt tttctttcaa
    45361 caaatcacga atttcgtcgc ttcggacaaa atcacgattg cttcttgctt gatttcgttc
    45421 ttcgatcaat tgttcaatct cttcatctaa taactcttct gctgtaaata aaataccgaa
    45481 gatcgaaagc cattgcgaga ataaagcttc tccttgtgcc aagacagcgg cagaaacttt
    45541 cggctggtct agataagtgt tcagccactt agcaagctca tagaccactg tgatcccatt
    45601 tgctgcatta aaatcatcgt ccatttcttc gatgaatcgc tgttctaaat tttctaacaa
    45661 agcgatttta tccgcatcat ctgccaaatt ctctgaggca gtttcttggc ggaatttcaa
    45721 attgtcaaac gtattcttca gtcgttgtaa attggtggct gcttctttca tcgtcgtttc
    45781 actgtagcgg atcggacgac ggtattgtgt ggtcgccatg aagaaacgca agacttgtgg
    45841 atcaacgttt ttgatcattt catgtacagt gatgaaattc cctagagatt tactcatttt
    45901 ttcatcgtct tcgccaatcg ttacatagcc attgtgcatc cagtaattag cgaatttttt
    45961 ccctgttttc gcttcacttt gtgcgatttc attttcatga tgaggaaatt ctaaatcttg
    46021 cccgccacca tggatatcga tcgtttctcc taaatgtttt gttgccataa cggaacattc
    46081 aatatgccaa ccgggacgtc ctttgcccca tggtgaatcc caagaaatct cgccaggttt
    46141 tgcttgtttc cataaagcga aatctaatgg gtcttctttt tgctgctgtt cttcccctgt
    46201 acgctgactt gctccgactt ccagttcatc gatggattgg tcacttaatt tcccataatt
    46261 ttcaaattta cgggtacggt aatacacatc acctgaagat tcataagcat agcctttttc
    46321 aaccaacact tgaataaatg ctaaaatatc tggcatatga tccatgactc ttggatgcag
    46381 tgtagcaggt tcgacattca acgctttcgt atcttcttca aatgctcgga tgaaacgatc
    46441 agcaacttct ggtgctgtaa tacctaattc gtttgctgta cggatgattt tatcgtctac
    46501 atccgtgaaa ttcgatacga aatcgacttt atagcctcga tattcaaaat agcgtcgaat
    46561 tgtatcgaaa gcaatggtgc tacgagcatt tccgatatgg atataattat acacagtcgg
    46621 tccgcagaca tacataccga catgtccctt ttgtataggg atgaattctt ctttttttct
    46681 ggtcaatgta ttgtagattt ttatcattct gatctcctca ctttttctcc tttgattcga
    46741 acgacttttg ctggtactcc taccgctgta gcgtcgtctg gaacatcttt caacacaacg
    46801 gcacctgcac cgattttggc gttttttccg atcgttaccg gtcctaatat ctgcgcattc
    46861 gcagagagca tggctccttg tttaacagtt gggtgtcttt tacccgtttc ttttcctgtg
    46921 ccgcctaatg ttacgccatg gaataaaatc acatcgtcct cgatttctgc ggtttcgcca
    46981 atgacgattc ccattccatg gtcgatgaac acgccagttc caatcgttgc tcctggatgg
    47041 atctcaacac cagttaggaa acgccagaat tgagcattga tcttcgccaa taaaaagagc
    47101 cggtgctgat ataaaaaatg agagaaacga tgccaaaaca aggcatgaaa accagggtat
    47161 gtcaaaacga tctcagctgt cgaacgtgca gccggatcgt ttctctttgc tgcatcaatc
    47221 gctcgggtaa accaactcat gactttccac tcctttcgta agaattttat gtaggaaatc
    47281 atcttcttat aaagaaaaga agcgcctttt aaataagttt ccttatcaaa agacgctttc
    47341 cgcgtggttc cacttttctt cataatcaat gattatctct gacagctaac gcctgttata
    47401 cgttcttggc tactttgttt cctataaaac agtttttcct gttttctgtg tttcgccaag
    47461 accactccca gaggcatttc ggcaaccatt cattgatacc ttgcaccacc cggtatctct
    47521 cttgtaatga tcagctgctt acttttttct gttcatcgtg tttagttcag tacttgattt
    47581 aaatgtgcta atgatttttc acgaccaagc aattcgatcg tttcaggcaa ttctggacca
    47641 tgcatttgtc ctgatacagc tacacggatt ggcatgaaaa gattttttcc ttttacgcca
    47701 gtttcttttt gaacagcttt gatggcagct ttgacagttg gagcatctac tacttccatt
    47761 tgttctagct gtgctttgaa tgcgtggaga acagtaggaa ctgtttcacc agcaagtact
    47821 tcttttgctg cttcattcaa tacaggatgt tcattgaaaa atagcgtaga cagatcaacg
    47881 atttctgcag catagctcat ttgaggttga tacaggctaa cgatcttctt cacccattcg
    47941 attgtctttt catctgggtt ttctgctaca cgtccatcag cgattagata aggcagacac
    48001 atgtcagtaa atgcatctag atccattgct ttgatgtaat ggttgtttac ccattgcagt
    48061 tttttcgcat cgaaagctgc gggtgattta cttaggcgtt ttggatcgaa cattttgatc
    48121 aattcttggt gttcgaagat ttcgtcttcg cccacaggtg accagcccaa taatgcaata
    48181 aagttgaaca ttgcatctgg tagatagcct aattcgcgat attgttcgat aaattgcaag
    48241 attgattcgt cacgtttgct tagtttttta cctgtttcac tgttgatgat caatgtcata
    48301 tgtccgaagg ttggtggtgt ccagccaaat gcttcataga tcatcagttg ttttggtgta
    48361 ttggcaatat ggtcatctcc acgtaaaacg tgagtgattt tcatcaaatg atcatcgacc
    48421 gcaacagcaa aattataagt tggcatacca tcgcgttttt ggatgacaaa gtcgccaccg
    48481 atattatcgg attcgaacga aatagcgcct ttcaccatat ctgtaaatgc gtattctgta
    48541 ttgcgaggca cacggaaacg tacaacagat tctagacctt gtgcttcttt ttctgcttgt
    48601 tcttcaggtg tcaagttcgc acatgttcct gcgtaatgag gcatttctcc tcttgcgcgt
    48661 tgtgcttccc gttctgcttc taattcttct tctgtgcaat agcatttgta tgctcgattg
    48721 ctagcaagca gctgatcaat cagcggttga tagatttctt tacgttcaga ttgacggtaa
    48781 gggccgtatt ctccaggatt ttcaggagat tcatcccaat tcattcccaa ccaagcaagg
    48841 ttttccaact ggcttttttc gccatcttcg atattacgtt tttggtcagt atcctcaata
    48901 cgaataataa agtctccatc attgtggcgg gcaaacaaat aattgaataa tgcagttctt
    48961 gcatttccaa tatgcaggtg cccagttgga cttggtgcat agcgtacgcg tactttcgtc
    49021 attttcttcc tcttctttca aactagactt tgactaacag caaaaattcc gtcagttacg
    49081 gcttctcaaa tatcatttat ttaaagccac tcagcacaaa ttttaccttt tctaaggtaa
    49141 atagtaaagc caactggttg aatttcctta ccagttggct gaatttcttt tttattttga
    49201 ttgtttagtc tttttctctg tctctttttt attgtcgatt cctcgtcctg catgtgcagg
    49261 tttagcaaag atcatgcgtc cagcagctgt ttgaagggca cttgttacaa cgacttcgat
    49321 acgttcattc atgtaatgct gtccatcttc tacgacaacc atcgtgccgt catcaaggta
    49381 ggctactcct tgttggcgtt ctgtcccggc ttttactact agaacattca tcgtttcacc
    49441 agggatgacg actggtttta cggcatttgc caacgcattg atattaagaa caggtacatt
    49501 ttggaattca gagactttat tcaagttata gtcattggtt acaatgacac catctaaaag
    49561 cttagccaat ttgatcaatt tgctgtctac ttcactgata tcttcaaagt ctccatcgta
    49621 catttcgaca gatataccct cttctttttg caaggcattt aggatatcta atccgcgacg
    49681 accacgcaca cgtttcaaac tatcccctga gtccgcgatg tattgtagtt cgtaaagaac
    49741 aaaattaggg atcatcaagg ttccttctaa gaatccagtc tttgtaatat cataaatccg
    49801 accgtcaatg atcacgcttg tatccaagat tttatattta tggaaatttt ctcctgattt
    49861 tcgttctaac agttgactct caacttgact agctgtttct tgctcgattt ttttgcttcg
    49921 tgaaccaaaa actttcttcc attcttcgat tcgtgtcgtt cccattctca atcccagata
    49981 gcctaagatg atcatcagta aaatcggcaa tacactattg acgaatggaa tttccatatt
    50041 gtaaagaggg gtcgagatca caacacctaa agtcaatccg atgatcgcac cgatcgcacc
    50101 gaagagaaga taagtcaagc tgacctcact tagtttttgt tctacttgtt tgataatagc
    50161 tgagatatgt ttggccaaca gcaaagataa taaaaagaaa ataagtgcgc ctaacaggct
    50221 gttggtaaat gtattattca gccatccgtt tgactgttga ccgattgcct gccaagctga
    50281 gggtaaaaat gaaataccca agcttgcgcc gacaatgatc atcagcaaag taatgacacg
    50341 tttttgcatc gtttttcctc cgtttcgaca atcatttatt agttagcgga acacacgctt
    50401 caacgtttca gccaaagttg ctactccaac tatctcgatt ccttttggcg cttcccagtt
    50461 acctaagttg tttttaggta ggtatacttt tgtgaagccg agtttttgga cttctttgac
    50521 tcgttgttca atatgactga cacgtctgat ttctccagtc agtccaattt caccgatgaa
    50581 acattccgtt ggctgtgttc ctttttcttt atagcttgaa gcaatactga cagcgatagc
    50641 taaatcaatc gctggttcat ttaattttac cccacctgca gcttttaagt aagcatcttg
    50701 attctgaaga agcaacccag ctcttttttc cagcactgcc atgatcagag atacccgatt
    50761 gaaatcaaga cctgttgttg ttcgtttggc atttccgaac atagtaggtg tgactaatgc
    50821 ttggatctct accaaaattg gacgtgtccc ttccattgcc acaacgatag cagagccagt
    50881 tgcaccgtct aggcgttctt ctaagaaaat ctgtgaagga ttagcgactt cttccaatcc
    50941 atgctcatgc atttcaaaaa tcccaatctc attcgttgag ccaaatcggt tttttactgc
    51001 tcgcaaaatc cgaaaactat gatgttgttc accttcaaaa tataacaccg tatccaccat
    51061 atgttctaac attcttgggc cggcgatcga gccttctttg gtaacatgac cgacgataaa
    51121 aatggctatc ccatttgttt tagcggtttt gagtagttct gcagtggttt ctcttacttg
    51181 gctgacactt cctgccacac ttgtgatgtc tggctgcgtc atcgtttgga tggaatcaat
    51241 gataacataa tcaggggaga tatgttcgat ggcacgactg atttcgttca tatctgtttc
    51301 agcgtataga taaaattctg tgttgatact tccgaggcgt tccgcgcgta attttatctg
    51361 ctctgcgctt tcttcgccgg atacatataa gacttttccg cctatggctg ctaattgctg
    51421 agaaacttgt aacagcaaag tggatttccc gatacctgga tcgccaccaa tcaagaccat
    51481 agaccctggc accacacctc cgcctaacac acggttcagt tcttctaatt ttgttttgat
    51541 ccgaggttct tttttaggga cgacgtctgc gatttttgtt ggttttgctt tttctcctgt
    51601 caaacttgtc cgagttctgc ggtccgttgt atcttgttcg atttcttcga ccatcgtatt
    51661 ccattttccg caatttgggc agcgtcccaa gaacttaggc gacacatacc cgcaattttg
    51721 gcaaacaaat tgtacttttg atttttttgc cattgtaccg cctcctttta tcctaatcat
    51781 gagacattaa atcaagctta agtatcttta acgttttatt tactggaaga accaaatccg
    51841 ccttgacgtt gagcagtcgt ttgatcttga tctgctttta aaaacggcaa aaagatccct
    51901 tggccgatac gctcgccttt tttgatcact acatccttga aaccaaagtt ggtgaattgg
    51961 aacatgatat ggccttcatt tgcctcatta ttgtaataat cactgtcaat cacacctact
    52021 ccgtttgtca acactaaaaa ccgcttcaac ggattgcttg aacggttagc aagctgcaaa
    52081 aattcatctt ctcccatgta agctttgatt ccagtaggca cgagggttgg tttcaattgt
    52141 ttttcatttt ctgatacatc ttttttcaat ccatctgcga ctgtttccaa cccctttttc
    52201 cagcctgctt tccagatact tggaatcacg acctcttctg ctgcttcaaa atcatagcca
    52261 gcagcatgat gtgtagcgcg ctgcggtaga ttgatatttt gatctttata tttagaaata
    52321 acttcaaatc ctctttttct caccggtttt cctccctaaa taaactactc ctattctaac
    52381 atatccgagg ctgtaaaaga ctttctttac aaattcttca agaaattctg ctatcttaaa
    52441 gtaaaccatt aattcgttct ttttccaaac gactgcaagt tgtacaaaaa aactattttt
    52501 tacatgactt tactcgtcaa aacaagtaga atagaacgaa attaatgagt ggatatttga
    52561 ttgaggtgtc acatgcgtaa agaaattaaa aatgcaaaac gaattattgt caaagtagga
    52621 acaagtacat tgatttatcc caatggaaat atcaatcttg cggcaatcga tcagttagct
    52681 tttgtccttt ctgatcttag aaatcgggga aaggaagtga tcttagtctc atcaggagct
    52741 gtaggcgttg gtatgcataa attacatttg tcagaacgtc cgactttgat tcctgaacag
    52801 caagctgcgg cggctgttgg tcaagccgaa ctgatgaata tctataatca aagattcagt
    52861 gtatataatc aacagacggc acagatcctc gtgacaagag acgtggtaga ttatcctgaa
    52921 agtcgtaaaa atgtgaggaa tacattggaa cagcttttga ttatggacac gatccctatc
    52981 gtgaacgaaa acgatactgt agcagtggat gaattagatc atctgacaaa atttggcgat
    53041 aatgatcagc tgtcagccat catggcaaaa cttgtcgcgg cagacctttt gatcgtcctt
    53101 tctgatatcg acgggtttta ttcagataat ccgactacaa atcccgaggc tcagatgtat
    53161 cgctatatcg ataaaatcga tgaaaagatt ctggcacaag caggtggtgc tggtagcaca
    53221 tttggaacag gcggtatgca gagtaaatta aaagccgctt ctaggatatt tgaagcaaac
    53281 agcgcaatga ttttagcaaa tggaaaaaat ccggcaatta tttttgatat tcttgcagga
    53341 aaagaagtag gtactttatt taaggaggac ttgaaatgac tgacttgatg caattaggac
    53401 aacaggcaaa agatagcgcg caacaactag cacttatgcc tacaaaccgc aaaaatgagc
    53461 tacttgttca aatggcacaa accatcaaaa agaatcaaca agcgatcatt gaagcaaatc
    53521 ataaagactt agaaaaagca gtcgaaaaca atatcagtga aacgatgcaa gaccgcttgc
    53581 agctgacacc tgagcgaatc aatgccatgg ctacagagat cgaaaaaatt gcttcgttag
    53641 acgatcctat tggaaaagta gatgagatgt ggacgaacac cgacggattg agaatcggca
    53701 aaaagcgcgt gccactaggc gtgatcggca tcatttacga atctcgtcca aatgtcacga
    53761 cagatgcagc tagtctagca ttcaaatcag ggaatgccgt tatccttcgc ggtggcaaag
    53821 aagcattctt ctcgaatcaa ttattagtcc aattattgca gcaagtactg gtcagtgctg
    53881 gcgaatctcc ttatgccatc cagtttgtcg atgatacttc tcatgaaact gcaggacagt
    53941 tgatgcgatt gacagaatac ttagatgtgc tgattcctcg aggcggagca aaattaatcc
    54001 aacgagtcaa agaacaggca acggtaccta tcattgaaac aggtacagga aataatcacg
    54061 tatatatcga taaagaagca cagttatcta tggcagtgaa catcatcgtc aatgcaaaag
    54121 cttcccgtcc atctgtctgt aacgcggcag aaacattgct gatccattct gagatcgcac
    54181 cttttttctt gccagctatt gaaaaagaac tggtagaaca cggggtctct ttacgtgcgg
    54241 atgctcgtgc gttagaatat ctagagacgg ctgctttagc agaagacaca gattgggata
    54301 ctgaatatct agattatatc cttgctgtca aagtggtcga ttctttggat gaagcaattg
    54361 cacatatcaa tcggcataat actaaacatt cagaaacgat cgtgacagat agttatgcgg
    54421 ctagtcaacg tttcttaaat gaagtggacg cggcggctgt ctatgtcaat gcctccactc
    54481 gttttacaga cggatcggta tttggttttg gtgcagagat tggtatctct actcaaaagt
    54541 tgcatgcccg tggtccaatg gggttaaatg aattaacttc cacaaaatat atcatttatg
    54601 gcgatggaca aatccgcgaa taatgtttcg tgtgaaacat tattcccgcc ttttgccttt
    54661 tttctgtaaa ggttctatcc aactagaaag gtgtcaatat gtttgaaatt ttaatgattt
    54721 tttttattta ccttatttct ctgaatatcg ctgcttttct gggagtcggt attttatccc
    54781 ttttctttca attcaaaaaa cgaagcatag ggagtcaacg ggaaaaatgg tcgcagtatt
    54841 ttgacaaaat cgggcaaaaa gggctggtta cacgcttgca cattagttac atggttgcat
    54901 tatgcttatt agcgacaatc aattactatt cttttttcga tcattcaatc gcttatacga
    54961 ttacgctact gattgctggg atttttcact tatcttataa atatcagctg aataaaaacc
    55021 acttgaatcg gactttccgc taaaaaatat ccgtacgata agtagagtgc cttgaagatc
    55081 ggcaaaaaac tgccggactt tttagaggct gtgacaaaga ttttgtcaca gcctcttttt
    55141 ccatataaac ggcgttcgcc taccagctgc ctggtaaatc tgtttcctct tcgttaagaa
    55201 gcgaaaatcc gtcaaatgtt tcgccatcat aataacgaag cagctcatcc atggacaagt
    55261 tcatcgtttt ttctttttct tcttccgctg attcagccag tagctgttcc cgatacacct
    55321 gatacatacc agctattttt tgctgcatcg cttttctagt ttcaccttcc gcattgatta
    55381 ttggcaattc tttgatatag ccacgataaa tcaaccgatc atctgaaaat tcaatgggta
    55441 attcaacgag tgtcaacccg acttcctgaa aatattcttc cagcatctat tatcccccca
    55501 ttctttttct taaatgaatt ttagcttacc tactgaaaat tttccagttt cattattttg
    55561 agaaaaaaag ggaatgtgac aaaaatcgtg tcacatcccc tttttccgaa taaatggtgt
    55621 tcttctgcca actcctcaat cataagtcac aatattcaac aaacatgaga aactgttttt
    55681 tgaaattgtt ccttgtgatt cagagttaaa cgacagtctc acaacctctt ggtaaacggc
    55741 gttcaatcag ctgaccctcg gtattagctc aaaaaacggt aaaatatgag aagctatttt
    55801 tccgttttgt ttctctaata cttaggataa aacgctgatt ttacaacctc tgttaaacgg
    55861 tgttcaatca gctgtccttc ggtattagct caaaaaacgg caaaatatga ggaactattt
    55921 ttccgtttgt ttctctaata ctcaggacaa aacgctgatt ttacaacctc ctgataaacg
    55981 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagaagcta
    56041 tttttgtttg gctgttctta tgacttgatg caggacagct ttttcacaac ctctgatttc
    56101 ttattcttct tcatttgtag caaaataatt acggtgataa atgatacaac atacactgat
    56161 aagaacaatg gcacctgcaa aataaaacgc atagctttca ccaatatgtt caccaatcac
    56221 acctgcaata aatggtgcta aagctccacc gaaaaagcgg acaaaactat agctggacga
    56281 agcaacgttt cgttgcagac ctggtatttc catagcaatc gtcgtcaaca gtgtattgat
    56341 aattccttgg aaaaatcctg ctaacacaat gctgatcggt acaaggactg gcatagaagt
    56401 tccagctcct aataacagca aacaaataat gaataagaca aatgctgcta aaattgtccg
    56461 ataagtcgta aacttccgct caagagccgg tgcaacgaaa atcgaagcaa tagccagaag
    56521 cacaccccag ccaaaaaata caaaaccgac ttctaattca gaatagccaa tcaataaaaa
    56581 tggcgcatac gcaagtaaag taaagaatcc gaaattatat aacaatgcga ttagtccaac
    56641 tgaacgaagt ttatgattgc ttaacgcttt gacaccttcc caaaggccta cttttctctt
    56701 tggtttctca gtttcaggta aaagtaccgc tagggctata aaaccgcaaa acatcaaaaa
    56761 tgctacccca aaaaatggat aacgccatga accactgccc aagaccccac cgactagagg
    56821 gccaacagcc attccgcagc caagtgctgc ttcatacatc atgattgctt tttctgtgtt
    56881 accaactaaa atactgacga tcgttgctaa agcagtagag acaaataaag cattcccgac
    56941 accccagccg gcacgtaacc caatcagcat tccgatattc ccagataatc cgccaagacc
    57001 agcgaataca acgatgataa acaatcctgt catcaatgtt tttttcaaac cgatccgact
    57061 agataagaaa cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa
    57121 cagggtcgtt tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc
    57181 tactagaccc actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact
    57241 tttccccttt gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc
    57301 catccctttc tctctattag catcatacat gatttatttt acatgtcaat atgcacatgt
    57361 aaaacgtgga aactttactg tgatataata aagaaaataa gacgagaggt aggattttta
    57421 tgaatacttt agcctatata ttattatgcg tgcttttatc caaaccacgt tctgggtatg
    57481 aattgaagca gctgatcaca attttttggg aagcccatca cagtcagatc tatacaacgc
    57541 ttgccaagtt agaccagcag ggttatgtga ccgttttgga caaagacgag cactctcaaa
    57601 aaaagatcta ccacttgact gatgaaggac gtgctcttgt agaggaatgg atcaaagagg
    57661 aaactcccgc tcctacgcaa aaagatgaat ttctagcaaa aatctatgct ttttcgacgc
    57721 tcgataaaac aacagctact ggactgctat tcactagaga acaacagctg aacaaaatca
    57781 tgcaaaaaaa tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa gaatttggac
    57841 gttatgttgt gatccagcgc agaattatgc tttgtcagca ggaactccaa tggtgtcagc
    57901 aagtaagaga acaaatgcag cagttttttg agtaagggat aaacagaacc tttctagaaa
    57961 tatctattga agagcaaatt atcttcaata aaagagcaat acagttggaa aagaaaaagc
    58021 gatccgaacg acaggccaaa acctatcgtt cggatcacta tttttctaga actgcattca
    58081 agagactatg acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt ccatcattca
    58141 ttactttgaa ttgcttttag cacacttctt gaaagcgggc caacgatgat caactgcaac
    58201 ggtaaagcgg caatcacatt cattttccaa gcattcatat aagcagagaa taattgatca
    58261 ggtattcctt gttccatgat cacaccgaat aaagacatac atgtcaccat tcctaaaacc
    58321 atcaagcatg aaatagttaa gatcatttgg accttgcttt ctggatttac tggtagttta
    58381 aaggcaattt ttttcgccag cacaccgatg atgaagacat ccaaaatgaa agccaccacg
    58441 aatccaggca ctagaccaat caacaaactt gataaatgaa gatcttgatg cagccataaa
    58501 ttatacgcgc tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa aattattcct
    58561 tcttttttat ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta
    58621 gcacatttaa aaatttctcg taagtacttt ttttattttc tgccctttta tttcataaac
    58681 ttcatcagcc gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg
    58741 atatatttgc attagcttct ctaaagcttc gatcgttgcc atatcaataa aattagtcgg
    58801 ttcatctaac agcaacacat tacttggctt agtaaaaagc aaagcaatcg tcagacgggt
    58861 cgcttctccc ccacttaatg tacataacgg tttcgtcaaa gtttcctgag caaaccctaa
    58921 gtgattcagc aaactacgga caagcgattc tgaaaactct gtttgtctca tgacaaaacg
    58981 aatgatggat tcttcagatg tcatttgata acccttttgc tgatatactt gaaagcttac
    59041 tttcggagag agaaggattc cttctccttg tttgcggata tgttctaata aggttgtctt
    59101 acctgaacca ttttcaccga caatcgcgat ttttttacct aaagaaaact gaaaatcacc
    59161 atttaccagt aatgttctcc cacttctttc caattggaca ttttgtccca tgatgggata
    59221 gcggctgtgg agttcaagag attttggtac tggaaaacgg atttgtttca tctgctgcgg
    59281 cttggttgtt tctcctattt tttggagtct tctctctaat gttttcgcat tcttttggat
    59341 ggctttttgt acactgtctt tttgtttaga ggaagacaac cgatccagtc tgatttgttg
    59401 ctttttcttt ttactggaca ctttttccaa cttttccgct tgttttttct tttcttggat
    59461 cgatttttcc aatcgttttt tctctttcat gatcgactct gcttctcgta gctgagtctt
    59521 tttctccaat tccttttgag tgagataggc actataattc cccgaaaact ctcggatttc
    59581 tccgtctttt acttcccaga ttttcgatgc tagctcatct agaaaatagc gatcatgact
    59641 aacaaaaacc agcgtaccat aataataacg aagctcttca atcagtcgat caatactttt
    59701 tctatctaga tgggttgttg gctcgtctag aagcatccct tgttcataag aagaaagaag
    59761 acgtatcaac tcgatctttc gttcttcccc tccgctccat ttccgctctg aagtttcttc
    59821 cagtcttaac tggaaatgac tggctaattc caaatttagg tcctttgctt cagcgagata
    59881 ggttaattga ggcaggtagt tgaaggtgat ctctgtttgg attttcccat gatcgggaaa
    59941 aagttcaccg gctattagtt tgagcaaagt cgatttaccg acaccatttt ctcctatgat
    60001 ccctatacga tcttgctgat agacacgaag agaatcaata gatagttcta gctggttgcc
    60061 aaagctgact tgtagatttg ttatatttac tgctaaattt tccatatgtt ttacactcct
    60121 atttcatgta aaacaacaat tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa
    60181 aaactgcatc acatcttttc ctactctgaa cgaaataact atgactaaat caccaaatct
    60241 gtcatagctt tcacctattc tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa
    60301 ttgttgttta tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa
    60361 tttcatcgat gcagtcattt ttccgacctc cttttttatt aggttaccac acacgattca
    60421 aaaaaagcaa tagctactga atagtaactg aaaacctttt attcgaacat agtgatcttt
    60481 tctggtgcta aacgaacaga ctcgtaacct tcttctaccg cttttcctat tgataaaata
    60541 ataactggaa cataacggtc tttatccaaa ccaaatgcct ctgccaactg atctgcttcg
    60601 aatccaccaa ttggatttgt ttcatagcca tgatcacgcg caactaacat gaattgcata
    60661 gccgctaagc tagaatcgac tttgactaca tcgttcattt cttctcttga aaaactcttg
    60721 taataaggaa tgatcgcagc taattgttga tctctgactt cttttggcat cttccctgat
    60781 tcgtaagctt ggttgtagat ttcttcgcca tattcgtaac attgcatatc gccaaaaatc
    60841 atgaccattg cagaagaagt atcattttgt ctcgtattga aacggatcaa aggacgcaag
    60901 atttcttttg cttcctcgct ttcagcgacc acaaagcgcc atggtttcat attgacagaa
    60961 gaaggtgcag tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg gattttgtat
    61021 gtttcatcat atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa atcatttttt
    61081 tgttccatta ttattaacct ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa
    61141 tttatctaaa agtgttagaa attgtttcat ttctacatcg ctgaaagccg gggatagcac
    61201 ggaacctgta tctttatggt ccatttcaca atgcttgatt ttcttctggg ctttttctgt
    61261 tagcgctaca tacacttccc ggttgttctg aggatttctc tgacgcgaaa catagccatt
    61321 tgcttccaat accttcagat gacgagtaac tgctccttga tcaatctgca acttttcttg
    61381 caaactgctt tgtagcaatg gattttcttc tttcagtctc atcatgatct cgtatctagt
    61441 aagacttata ccgatatttt cttcaaagat ctggacgatt ttttgatcgg ctaatttgat
    61501 ttgatataaa aggtgactaa tctcttctaa agacataatt tcctcctttc taataattga
    61561 ctcgtcaacg attgacttgt caataataga ataaaaaaag aaaaaagtca aagaatcagc
    61621 tgtgtaataa aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa
    61681 tgaacggaaa aaatgataaa gaaagcacaa cgctaaaacc aagattattg attcttatca
    61741 aactatagaa gatagctctt ctgcagtaga taattttaca ccgccaaaaa tttccgaaat
    61801 cgccttttgc aagaatcccg tcgaatatta gaaattggat cagtatgctt aaacagtaat
    61861 aaaaaagtta tgacaagatg ttgtcacaac cttttttgag taaacggtgt tcaaaaactt
    61921 gcatctgcgg taataagttc aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg
    61981 ttcttactac ttgatgcagg gcagcttttt cactacctct ttttaataac cataaaatcc
    62041 tattccttca tatctccagc ccatcggtgt tagccaatct ctttccttag cactcaaggt
    62101 atagtgatgg gtaccagttt tttcatttgg attaaatagt cgataaattg gcttggaacc
    62161 accagaataa aaacttagac cttcataacg ccaaccgatt tttgttaaag agttgatctc
    62221 agattgtgcc attgtgtaat gatggtctcc actgtaagga ttgtataaac ggtagacagg
    62281 ttgtccgctg tttggtgctt gccaagcaat cccttcatat tcccagccta cagttggcag
    62341 ataatttttt tctccggcat tcaatgtata taaatgttct cctgtatgac ggttatacag
    62401 acgatacatc ggagaagttg aagttggtgc tgtatcatac tgtgtcagat tataggtttc
    62461 gattacattg ttcaatgtag aaccataatt aggcgcagtg gcataacgac ctgttagcca
    62521 agccgtagca tctttgtaag agttcgtatt gcttttccat gcaccggaat agtagtaaac
    62581 gcctggctga aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg attctttgta
    62641 cgaaggatat tttctaaaag cggcattcac cgtaatccat tgcccattca catattccga
    62701 agtaggcatc gtaacagatt ggccattata gcttccttta atcccaaata gattgtaatt
    62761 aggggcagaa gccaaagtac tgttccccca gccactttca acgatggctt gagcaatcat
    62821 gactgatgca tataagtcat tggcacttgc caaactttgt gcagttgagg caatactatc
    62881 aatgaacgct tgttgagaat tagcagatga gcgagcataa aaagcatagc cagtatagtt
    62941 ttcgaattca gacaaatctg cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg
    63001 gttcgtgatt tcttctgcat tctctggatt gttctgagct tctgctgatg tactgtcttc
    63061 ggtttgtgtt tgttctgttg tttcagttgt tggttcttcg gttgaaattg tattttctga
    63121 gacagtagct gactgcaaag tactttctgt atcgttttca attcctgttt ctgaagaact
    63181 cgatgtctga gttgcgacag tttctgtcga ttcttcagct agcactgttg tgttggcaac
    63241 tgctagattt cctaaaagaa taacgctaaa taataaattc tttgctttca tcttttttct
    63301 cctttgaatt atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca
    63361 atgtccatat ttaaataaca tttgtttctt tttaataaat ttgctttttc tgcataaaaa
    63421 gacgtaaatg aagaatattc ttctccattc acgccttaaa ttgatggtcg tttgaataac
    63481 tatttaacaa gtaattctct ctggatatta tccgttatgg attatacttc ctgtgtcgtc
    63541 ggacggataa tcatgttgct gacagacatc cggtctggcg tttctatcgc aaatgctaca
    63601 gcagaagcta tatcttcaga tgttaatccc cattcttttt gcgcttcatg aagttcaaga
    63661 gcagcctttt tgtcgctgat ggtttgatat aattcagttt gaacagctcc tggagagatg
    63721 atcgtagatt tgatgttatt ttgccgttgc tcttgacgta atccttccat gattgcacgg
    63781 acagcaaatt ttgttccgca ataaacggcg gaatcaggat aaacaacatg tcccgccaca
    63841 gaatcagttg aaatgatatg cccagatttt tgtttttcca tgattggaag tacggcggaa
    63901 attccattta acacacccat gatgttgata tcaagcatat tttgccattc atcacggtga
    63961 ccttcaacta atggagcggt tggcatgatt cctgcattat tgtacaatac atcgattcga
    64021 ccgaactttt ccatcgctaa atcgataact gcttggactt gtgcaaaatc tcttacatct
    64081 gctgtttgaa tatatagctc agcttctggt aaagattctt ttattgcttt taggcgatct
    64141 tctcggcgtg cagctataac tagtttagcc cctttttctg ctaatagctt cgttgttgct
    64201 tcaccgattc cgcttgaagc gcccatgatg acaatcactt tttctgataa atcattcatt
    64261 ctattctcct cctgttttga aaaatctctt gcttatactc actatctctt atttcatctt
    64321 actgttcaaa taatccgttc gacaggtttg acgattttgg cagggattcc tgcaacgatg
    64381 gtattgtctg ggacatcttt cgtaacagtt gcattcgctg caacaatggc attctcgcca
    64441 atcgttacgc ctggcagaat agttgctcct gctccgatcc aagcattttt cttgatgtgg
    64501 attggcttta ctgacaagcc tcttcgttct tttggatcgg ttagatggtt caccgtgatg
    64561 aggttgacac gaggaccaat caatactgaa tcttctaaac agatgccacc taagtcaacg
    64621 aacatcactt gacgattgat aaagacattt ttccctatac taatatgttt gccgaaatct
    64681 gtataaaaag gcagcagaac agtcaccgta tcatctactt tgtcactaat gatattttct
    64741 agccgtttgc ggatatctgc tggttcatgg tacccagtat tcagcgctgc taaaggctct
    64801 tcgttttccg cttggatctg atggatcact tcaaagagcg gatccgttac tcgaatctct
    64861 ttattgacga tttgtttttc gatgaggctt gtcattttgc tccctccttt tatccttact
    64921 ttataacgga cggcttatga tgtctaatac ttataaaaca tacttaacta ttccttttaa
    64981 ctatacttag tggtatgctt ttttaaaaac aggagggaaa aaatggatat tcgtatactg
    65041 acgtattttt tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa atctctgcat
    65101 atcacgcaac ctactctctc aaaacaactg aaagatttag aagaagaact tggtgtgaca
    65161 cttttcactc gaggctcccg agagatacag ttgacggatt ctggtcgata tctttattca
    65221 aaaggaaaag aaatcttgtc gttggtagaa aaaacggcga acaatttaac aaatgacgca
    65281 atcatctctg gagaaatcta cattggcgga ggcgaaacta aagcaatctc tttcatttct
    65341 gatagccttc attcgttgat caaggcgcat cctgatatcc agtttcattt atatagcggg
    65401 aatgcagatg atatcataga caaactggat agcggattac ttgattttgg actcgtaatc
    65461 gaaccgacaa acaaacaaaa ataccagtca tttcgactgc cagcccaaga tcgttgggga
    65521 cttttgatac atcggaatca tcctttagca aagcaaacag tcatccatcc gcaggaactg
    65581 aaaactgttc ctttgatcat ttcccggcaa acgtcagtgg acagtcaact agctgaatgg
    65641 ctaggtttca gcatagatca gttatcgatc gtcggaacat ataacttatt gtacaatgct
    65701 tctttgatgg tcgataacgg gacttttggt gcattagctc tagatgggat catcaatact
    65761 tatgggacgg aaaaagtatt catcccactc gatccgccat tacaagcttc actgaatctc
    65821 atttggaaaa aagaccagcc tctttctcct gctgctcgta aatttttaga tgtcctaacg
    65881 gagaaataca gttaagtaga gtttttttcc ttcgctttca gctttttaac aacgaataag
    65941 ttaagcaaaa gatccaatga ttaaataaca taagtttctt aatttgttcc ttccttttat
    66001 ttctgataat tccagtaaat tactataatt ataaaaaaag aattgacaaa acataactgt
    66061 ctatataaaa ttgattacgt taacaataat tcgtttcatg tgactagatc atactaggca
    66121 tgaaaaatct ctgaggaaca tggtttccct aggaggtttt tcgtgtctat ttttgtttgg
    66181 ccgcaatcaa ctggtctagc aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga
    66241 aaatcaaaaa gatctttaat caaaatgcag tattggtaga tgacggtgga caggaaaaag
    66301 tagctattgg aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg
    66361 atgtcgaacg gatatttgtc atggaaccag aaggtcaaat caagttgcaa aatttactta
    66421 atcagattga tgaacgcttt ttatttgcag cagaaacgat cattgatcat gcggaaactg
    66481 ttttgatgga aaagttgaat gaacatgtat tgatcgcttt gaccgaccac attgcttttt
    66541 ctgcggagaa cattaataac ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag
    66601 tgctttacag tgatgaattc agtattgctc aatgggcagt agagtatctg acaaaagagc
    66661 ttgggattcc atatacttat gacgaagcag gatatatcgc aatccatatt cacagcggac
    66721 gaagcggtcg gaacaataac catcgcagta tccgtgaagt gacgattgta tctgacatca
    66781 tccatttagt ggaatcagaa ttggaaacag atatccattc agaaccattt tcactgaatt
    66841 attcaagact ggtgaatcat ttacgactgc ttctacaacg aacccatgcg caacaatatg
    66901 ctgttttaga cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa agctataaga
    66961 tttctaaaaa aatccgtgtg ttgctgacca aagaatacca gctcgcgatc acgaaagaag
    67021 aactgggtta cttggccatc catatcgagc gtttaagaag cgcaatcgta caaacaaatg
    67081 ttaacaatca tgaggaggaa aaaaattgaa agcatatcta caacgaatgg gacgttccct
    67141 tcaacttcca gttgcggtct tacctgctgc tgctttacta gtagggatcg gaaactggtg
    67201 ggcatcttat agtaacgaca ttgtcgctca ttttttacaa gctggcggta acgcagtttt
    67261 aggtcaatta cctttgctgt ttgccgttgg gttagcacta gggatgtcag tggacaaatc
    67321 aggtgctgct gccttggcag gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac
    67381 tgattcagta gctactttat tgaatatcaa accagaatca gttgatccat cttttgctca
    67441 gatcagtaac gtatttattg gtattattgc cggattgatt gcggcagctc tttataatcg
    67501 tttccacgaa gcaaaattac cgatggcttt atctttcttt agcggcaagc gtctagtgcc
    67561 aatcttggct gcctttgtca tgttgctgat cagtgcagtg ctactgctcg tatggcctcc
    67621 ggtttactct gctttagttt cttttgctaa attcattttg agtttaggct ggggtggtgc
    67681 aggtctttat ggcttcttca accgtttact gatccctaca ggattgcatc aagcaatgaa
    67741 ctccgtgttc tggtttgacg tagcaggaat caatgatatt ggtaaatttt tggcttctga
    67801 aggaacaaaa ggcgtaacag gaatgtatca agctggattt ttcccaatca tgatgttcgg
    67861 gcttccagca ggagcatatg ctatttacag aaatgcacgt ccagaaaaca aagcaaaaac
    67921 agcttccttg atgttagcag cagcttttgc ttcattcttt actggtgtaa cagaaccact
    67981 tgaattctca tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa ttttcactgg
    68041 gatttcttta gcagtgagtg cattcttcca ttggacagca ggatttgcct ttagtgccgg
    68101 atttgtcgat tacttcctat ctttgaaaaa tccgattgcg aatcagccat tgatgttgat
    68161 cgtccaagga ttagtaacag cagtgattta ctactttggc tttaactttg ctatcaaaaa
    68221 attccatctg atgactcctg gccgcgagga agcagatctt tcagatgacg atactgcaac
    68281 gactaattca gacaataaat atgcggcaca agcaagcaaa atctacgctg cattaggcgg
    68341 agccgacaat gtaacttcga tcgataactg tactacacgc cttcgtttgc aagtcaaaga
    68401 tacaggcctg atcgatcaaa ataaaatcaa agccactggc gtacctggta tgaagatcat
    68461 cgacggaaaa aatgcgcaag tcatcgtagg tacagaagta caattcgttg cagatgagat
    68521 ggcaaaacta catggtggtg ctgcaacacg acctgcacaa acaaacaccg tggttaaaac
    68581 agaaacattt gccggtggaa cgatcgaaca agatatttac gctatcgcaa atgggaaact
    68641 gattccaatc actgaagttt cagatgatgt attcgcggaa aaaatgatgg gtgacggcta
    68701 tgctgtatta ccagaaaatg gagaaatctt ctctccaatc gctggtacga tcacaaacat
    68761 tttcccaacg aaacatgctg tcggaatcca aacagatgct ggtatcgaag tcttgcttca
    68821 tatgggcatc aacactgttg acttaaaagg cgaaccattc actttatatg taaaagaagg
    68881 tcaaaaagtc gcaagaggtc aattgattgc tttagttgat cttgccgcta ttcaatctgc
    68941 aggcaaaaac acggatatgg tcgttgtatt cacaaatggc gacaaagtcc aaagcttaga
    69001 aatcgaaccc gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt ccaacaaagc
    69061 ctgagccatc gtcttcccca gactttttta tcgataaaca aacaggagct gagacaaaaa
    69121 tcttgtctca gctccttttt ccgaataaaa acggcgttct cctgccaact ccgcggtaat
    69181 aagtccagcc aaacgtaaca tatgaagagc tattttttat ttgactcttc ttattacttg
    69241 atgcagaaca gcttttttac aacctctgat aaacggtgtt caaaaaccag tttatcggta
    69301 tgaagtcaaa tttatgcaaa aatgtggaaa gccattttca caaaattttt cttcatactc
    69361 aaaactaatc ggctttttca caacctcttt atacattatt cctgatccaa tatttctttt
    69421 aatgctgctt caagagaagt taatggtttt cccaatactt tttctaaatc atcagaggaa
    69481 atagctaact gatccttttt aatatcatgt tggatcgcta aaaacatgtc agctgcttct
    69541 tgagggaatc cggcttcttt tagattttcg acaaacgctg catcacttgc ttcaataatt
    69601 tctaactttt tacctgtcgc acgctctaat gccttggcta attcttcata gtcagttaga
    69661 ttgccggata attctaagat ttctggagag tcactttcta ctagtacttt tgctgcagct
    69721 tctgcatatt cacgtttcaa tgcccaacct gtttttccat ttcctgcagc ataaacgaat
    69781 tgaccagttt ttaaagcctg cccaatgata ggtaattcat tctctacata ccaattattc
    69841 cgtaaaaatg tatgggaaat acctgaatcc ttaatcattt tttcagtgaa ttggtgatct
    69901 ggcgctaata cactgatcga gtgatcggca tctgcaaaac ttgtataagc gatataagat
    69961 accccagctg cttgtgctgc cttcactaca ttcttatgtt cggcttgacg atttcctgga
    70021 acgccagaga caaaaagcaa tcgatctatt cctttcaacg cttctttcat tgagtctaaa
    70081 tcatcataat caccaatacg tatattaaat ccagcttctt tcagtgcttc tcctttttct
    70141 tcactacgta ctagtacata gatttcattt tgtggaacca tcttttttaa gaattccagc
    70201 gcataaccac caaaacctcc agttgctcct gttactaaat aatgcatatt tgccactccc
    70261 tattcttttt atttgttaag atttacagct gtacatttat aaattacacc tttatcataa
    70321 agagtatggt aagataagtc aagcaaaaaa aatgaaagga tgacaactga tgaagtattc
    70381 tctgcaattt agtgatgcaa tccatgtact tgcctatatt gaaatgtttc aagatacccg
    70441 atcactttct agtgagatga ttgccagtag tatcgaaaca aatgcagcaa atgtacgtaa
    70501 aatcatgagt cagctcaaaa aatccggttt gatctttaca aaaaccggaa aaccgcagcc
    70561 ttcccttgcc aaaagtccag atcagatttc tttactagat gtctatcgca gcatcgaagg
    70621 taatacacag cttatccaag tcgatccaaa aacaaatcca aattgcgtcg ttggtgcgaa
    70681 tatccaagaa gctttagaag aaagctacgc tcggctgcag aaaaaggcag aagaagaaat
    70741 ggctaagatc acacttgata gtctgataca taaaatagcc gttttggaaa aagaaaaaag
    70801 accagaaaac acaggcctcg tagagagatt tttataattc tctctactgt gcctgcattt
    70861 ttctgatcgg aatcgtctac ataaattctt ttattctccg ggggacaaaa gccttgtcat
    70921 gccccttttt cctctctaat gctcaggtca aagcgctgaa gtcatgacgt cttgataaac
    70981 ggtgttcaag tagctgaccc tcggcattag ttctgaaaaa tgcaaaatat ggaaagctat
    71041 ttcccatttt tcctctctaa tgctcaggtc aaagcgctac tttcacgacc tctatattat
    71101 catggtttat tgactaccgg tatccagatt tctcccatat atttcccgtt ggtaactccc
    71161 atttcgacta cagcatttgg acccccaaca taggcgtaat cttctacttc tcttaaaaca
    71221 tgcccaaagg ccgtattcgt caaatcattt cttaattctt caggcgattc tgctaaagcg
    71281 attacaacaa gatacgttcc ttttgggaat gggatgattc gggtagtgtt tggaacatcc
    71341 tgactcgttt gaacaccgac atagtacatc attttcccgt tatagggttc gttgactgta
    71401 aagaaataat gattttcagc agcttgtttc aatgcatcta ttgttccatt accgataact
    71461 ttttgctcaa atgcttgttt ctctgcagcc aaaccgacaa aatccgtatt gctactcttc
    71521 aattccacac caatacccgt caccgtaaaa tctggttttt cttcgatcct atactttgcc
    71581 atttctccca ccctttctat tatattaagt tcatcttaca taaaattaaa gggaaatgac
    71641 acgattacgc tcttttttct agggatattt ttatttttta actatttttt tgctttcact
    71701 gcaatccaaa tctcactgta aacattactg aaatcctctc cccattttgt aaaagagatc
    71761 tcgggagctt cgactaactc ataatctgaa gaaggaagcc attctgcata gattttcccc
    71821 caagtatctt gtaaagtttg gggaaatggc cctttattag gaaaaatggc ccaagtgtgt
    71881 tcttctatag caagcatttc taaatcagcg tacggatttt cttttgtcgt agcaaagcca
    71941 atcatgtgca tcaaactgcc cttttcttcc aagcgggctc catcaaatcc ataagaaacg
    72001 ttgattactt gatttggata taaatctcct aacttatgca tctcttccct ttgagtctcc
    72061 gtgatcgtct gcgctaactt ttgtatttct tgattgactc cttcaaattt gacaggaact
    72121 tttttcgcca cacctgccac ataaaactgt tctttctttt cgatacgaaa ttccatcgat
    72181 tttcctcctc tgacatcaat aaaaaatgtc aagcgtggaa aagatttttg tatgccgtat
    72241 ctgctgattt ctgaaggcag atatcctgac caatcacgaa aagctcttga aaaaccttcg
    72301 actgtttcat atccatatct aaacgccgtt tctgtcactc ttgatccatc taggagatcg
    72361 cgattagcca aagccaatct acggtttttg atatagtcca ttagcgacat ccctgcaata
    72421 aacgtaaaga tgcgttttaa ttgatattca gagattcctg tattttcggc tattatttta
    72481 taggaaagtc cttctgtcaa atgttcttcg atgtaatcga tcaattggtt cagatcttta
    72541 agcatattta tcttcctcct tgcttgcctt tttatcatat tatcaaagaa agtcaattac
    72601 ttgatgaatc aggaaccaat ttagtcagat gtagaaaatg ttgtatggat ctaaagaaaa
    72661 ttctcaagca gcgtttcgta acggataatt ggtattatct gcctatctat gttttacctt
    72721 tagaaaaata aattttgggg aagacacatt ctaagtaaac aaagcggtaa aaatatcttt
    72781 tattccctta gttaatccgc ttactaccta ctgtacaaag caaattgttg ttcaaactgc
    72841 tgatcagaac gctgcttgga tttttctttg cgatacatca tacctctttt atcaattgga
    72901 acaattattt atttctaaca aaatcacttc atttttgtca gataaataaa aaacaa
//
LOCUS       DASJTC010000003        62895 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.003, whole genome shotgun sequence.
ACCESSION   DASJTC010000003 DASJTC010000000
VERSION     DASJTC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.003"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            271..2976
                     /locus_tag="U4B19_000163"
     CDS             271..2976
                     /locus_tag="U4B19_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEN2075934.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            3574..5397
                     /locus_tag="U4B19_000164"
     CDS             3574..5397
                     /locus_tag="U4B19_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEN2075935.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(5539..5724)
                     /locus_tag="U4B19_000165"
     CDS             complement(5539..5724)
                     /locus_tag="U4B19_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2075936.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            6200..6463
                     /locus_tag="U4B19_000166"
     CDS             6200..6463
                     /locus_tag="U4B19_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2075937.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            6463..6729
                     /locus_tag="U4B19_000167"
     CDS             6463..6729
                     /locus_tag="U4B19_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN2075938.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            6930..7418
                     /locus_tag="U4B19_000168"
     CDS             6930..7418
                     /locus_tag="U4B19_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEN2075939.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(7959..8630)
                     /locus_tag="U4B19_000169"
     CDS             complement(7959..8630)
                     /locus_tag="U4B19_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2075940.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(8627..9544)
                     /locus_tag="U4B19_000170"
     CDS             complement(8627..9544)
                     /locus_tag="U4B19_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2075941.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            complement(9541..10182)
                     /locus_tag="U4B19_000171"
     CDS             complement(9541..10182)
                     /locus_tag="U4B19_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2075942.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            complement(10186..11361)
                     /locus_tag="U4B19_000172"
     CDS             complement(10186..11361)
                     /locus_tag="U4B19_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2075943.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            11554..12585
                     /gene="queA"
                     /locus_tag="U4B19_000173"
     CDS             11554..12585
                     /gene="queA"
                     /locus_tag="U4B19_000173"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEN2075944.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13204..15843
                     /locus_tag="U4B19_000174"
     CDS             13204..15843
                     /locus_tag="U4B19_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2075945.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(16011..16709)
                     /locus_tag="U4B19_000175"
     CDS             complement(16011..16709)
                     /locus_tag="U4B19_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN2075946.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            16927..18072
                     /gene="tgt"
                     /locus_tag="U4B19_000176"
     CDS             16927..18072
                     /gene="tgt"
                     /locus_tag="U4B19_000176"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEN2075947.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            18169..18549
                     /gene="yajC"
                     /locus_tag="U4B19_000177"
     CDS             18169..18549
                     /gene="yajC"
                     /locus_tag="U4B19_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEN2075948.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            18854..21451
                     /gene="adhE"
                     /locus_tag="U4B19_000178"
                     /gene_synonym="adhC"
     CDS             18854..21451
                     /gene="adhE"
                     /locus_tag="U4B19_000178"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEN2075949.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            21680..22786
                     /locus_tag="U4B19_000179"
     CDS             21680..22786
                     /locus_tag="U4B19_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2075950.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYY"
     gene            22803..23831
                     /locus_tag="U4B19_000180"
     CDS             22803..23831
                     /locus_tag="U4B19_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEN2075951.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            23837..24316
                     /locus_tag="U4B19_000181"
     CDS             23837..24316
                     /locus_tag="U4B19_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEN2075952.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            24332..25627
                     /locus_tag="U4B19_000182"
     CDS             24332..25627
                     /locus_tag="U4B19_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEN2075953.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            25638..26462
                     /locus_tag="U4B19_000183"
     CDS             25638..26462
                     /locus_tag="U4B19_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2075954.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            26627..27526
                     /locus_tag="U4B19_000184"
     CDS             26627..27526
                     /locus_tag="U4B19_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2075955.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(27652..28521)
                     /locus_tag="U4B19_000185"
     CDS             complement(27652..28521)
                     /locus_tag="U4B19_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEN2075956.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            28674..30134
                     /gene="rhaB"
                     /locus_tag="U4B19_000186"
     CDS             28674..30134
                     /gene="rhaB"
                     /locus_tag="U4B19_000186"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEN2075957.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            30131..31387
                     /gene="rhaA"
                     /locus_tag="U4B19_000187"
     CDS             30131..31387
                     /gene="rhaA"
                     /locus_tag="U4B19_000187"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEN2075958.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            31401..32246
                     /gene="rhaD"
                     /locus_tag="U4B19_000188"
     CDS             31401..32246
                     /gene="rhaD"
                     /locus_tag="U4B19_000188"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEN2075959.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            32248..32559
                     /gene="rhaM"
                     /locus_tag="U4B19_000189"
     CDS             32248..32559
                     /gene="rhaM"
                     /locus_tag="U4B19_000189"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEN2075960.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            32641..33792
                     /gene="fucO"
                     /locus_tag="U4B19_000190"
     CDS             32641..33792
                     /gene="fucO"
                     /locus_tag="U4B19_000190"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEN2075961.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            33919..34677
                     /locus_tag="U4B19_000191"
     CDS             33919..34677
                     /locus_tag="U4B19_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEN2075962.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            34777..35421
                     /locus_tag="U4B19_000192"
     CDS             34777..35421
                     /locus_tag="U4B19_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEN2075963.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            35428..36435
                     /locus_tag="U4B19_000193"
     CDS             35428..36435
                     /locus_tag="U4B19_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEN2075964.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            36451..37293
                     /gene="kduI"
                     /locus_tag="U4B19_000194"
     CDS             36451..37293
                     /gene="kduI"
                     /locus_tag="U4B19_000194"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEN2075965.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            37313..38116
                     /locus_tag="U4B19_000195"
     CDS             37313..38116
                     /locus_tag="U4B19_000195"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEN2075966.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(38365..39429)
                     /locus_tag="U4B19_000196"
     CDS             complement(38365..39429)
                     /locus_tag="U4B19_000196"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEN2075967.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(39426..40511)
                     /locus_tag="U4B19_000197"
     CDS             complement(39426..40511)
                     /locus_tag="U4B19_000197"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEN2075968.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(40524..41501)
                     /gene="mvaD"
                     /locus_tag="U4B19_000198"
     CDS             complement(40524..41501)
                     /gene="mvaD"
                     /locus_tag="U4B19_000198"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEN2075969.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(41494..42438)
                     /gene="mvk"
                     /locus_tag="U4B19_000199"
     CDS             complement(41494..42438)
                     /gene="mvk"
                     /locus_tag="U4B19_000199"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEN2075970.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            42754..43413
                     /locus_tag="U4B19_000200"
     CDS             42754..43413
                     /locus_tag="U4B19_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2075971.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            43498..44403
                     /locus_tag="U4B19_000201"
     CDS             43498..44403
                     /locus_tag="U4B19_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2075972.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(44405..45037)
                     /locus_tag="U4B19_000202"
     CDS             complement(44405..45037)
                     /locus_tag="U4B19_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN2075973.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    45188..45313
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            45357..45881
                     /gene="infC"
                     /locus_tag="U4B19_000203"
     CDS             45357..45881
                     /gene="infC"
                     /locus_tag="U4B19_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEN2075974.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            45953..46153
                     /gene="rpmI"
                     /locus_tag="U4B19_000204"
     CDS             45953..46153
                     /gene="rpmI"
                     /locus_tag="U4B19_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEN2075975.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            46206..46565
                     /gene="rplT"
                     /locus_tag="U4B19_000205"
     CDS             46206..46565
                     /gene="rplT"
                     /locus_tag="U4B19_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEN2075976.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            46817..48154
                     /locus_tag="U4B19_000206"
     CDS             46817..48154
                     /locus_tag="U4B19_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEN2075977.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            48202..50331
                     /locus_tag="U4B19_000207"
     CDS             48202..50331
                     /locus_tag="U4B19_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEN2075978.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            50306..50995
                     /locus_tag="U4B19_000208"
     CDS             50306..50995
                     /locus_tag="U4B19_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075979.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(51403..52080)
                     /locus_tag="U4B19_000209"
     CDS             complement(51403..52080)
                     /locus_tag="U4B19_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2075980.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(52216..52410)
                     /locus_tag="U4B19_000210"
     CDS             complement(52216..52410)
                     /locus_tag="U4B19_000210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2075981.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(52460..52900)
                     /locus_tag="U4B19_000211"
     CDS             complement(52460..52900)
                     /locus_tag="U4B19_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEN2075982.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(52904..53749)
                     /locus_tag="U4B19_000212"
     CDS             complement(52904..53749)
                     /locus_tag="U4B19_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEN2075983.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            53898..55316
                     /gene="pepV"
                     /locus_tag="U4B19_000213"
     CDS             53898..55316
                     /gene="pepV"
                     /locus_tag="U4B19_000213"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEN2075984.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(55373..56320)
                     /locus_tag="U4B19_000214"
     CDS             complement(55373..56320)
                     /locus_tag="U4B19_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2075985.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(56543..56893)
                     /locus_tag="U4B19_000215"
     CDS             complement(56543..56893)
                     /locus_tag="U4B19_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN2075986.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            57093..58172
                     /gene="pepA"
                     /locus_tag="U4B19_000216"
     CDS             57093..58172
                     /gene="pepA"
                     /locus_tag="U4B19_000216"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEN2075987.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            58316..58636
                     /locus_tag="U4B19_000217"
     CDS             58316..58636
                     /locus_tag="U4B19_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEN2075988.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            58658..59122
                     /locus_tag="U4B19_000218"
     CDS             58658..59122
                     /locus_tag="U4B19_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2075989.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            59327..59932
                     /locus_tag="U4B19_000219"
     CDS             59327..59932
                     /locus_tag="U4B19_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEN2075990.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(59971..61260)
                     /locus_tag="U4B19_000220"
     CDS             complement(59971..61260)
                     /locus_tag="U4B19_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEN2075991.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            61688..62167
                     /gene="rlmH"
                     /locus_tag="U4B19_000221"
     CDS             61688..62167
                     /gene="rlmH"
                     /locus_tag="U4B19_000221"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEN2075992.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            62370..62895
                     /locus_tag="U4B19_000222"
                     /pseudo
     CDS             62370..62895
                     /locus_tag="U4B19_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT        19952 a        11505 c        13377 g        18061 t
ORIGIN      
        1 atttttgtag gggcaaagac aaaaaatgtt ttagcttttt tgcgtatacg tataatagat
       61 aaaaactttg gcataaaatt atctttccct tttatttcat gataaagtaa aggctgtaat
      121 ccttaattat ttctcaatat gtaagagatt acaatgagac aattgccgtg atatgtccaa
      181 aaattcgact tttttgaaaa aagccgattg aaaaaagtat gaaatacatg gtaaagtaat
      241 tcagctgaaa aaagaagaag ggggagaact atgcagtcaa tcaaaaatac gcttcgatta
      301 tatttgcttg actggaaacg gattttcaaa aacccgattg caacacttct gatcatcgcg
      361 atcatgatca tcccttcgct atatgcgtgg tttaacatca aagcattatg ggatccatat
      421 ggaaatactg gagagctgcc aattgctgtt tacagtgcag acaagccagc agagtttcaa
      481 ggtaaagaag tggccattgg ggagcaagtc atcgaatcgc ttcataaaaa taaacaatta
      541 ggatggcaat tcgttgattc aaaagaacaa ttagaagacg gagtccgttc cggaaagtat
      601 tacgcaggta tttacttgcc aaaggatttc tcagaagatc tgctgagttt tactagtggt
      661 gatatcaaga aaccaaaaat cgaatatacg gtaaatgaaa aaatcaatgc gattgcaccg
      721 aaaatcaccg ataaaggagc aagttcgatc caatcgcaaa tcacaaatga atttatcaaa
      781 acagctagtt cgactttatt gaaagtattc aatgagatag gttacgacat cgatacgaac
      841 ctagtaagta tcaataaagt taaagatatg attttatcaa cagatgaaaa tttggacacg
      901 atcgacggct acacaaaaca agtcctcgaa ttacaaagtc aactacctga aatcaaggaa
      961 aaactgaata aagcaaatga attcgtggat tacatcccta aagtagatga gatgggtgaa
     1021 aaagtcgttg cattgaatga caaaatgcct gagttgaaag atcaagcaaa aatcatcctt
     1081 gatttgcaag aaaaaatccc agagatccaa aatgccggta agcaactagc agagatcgac
     1141 ggcgattttg cttcgatcca agagacaatg aaccaaggaa tcgatgaagc aaaacaaggc
     1201 ttgacgatca tccaacaagt acaagatatc ttgccagatg tgaaaaattt agaaaatcag
     1261 gcaagtgatc tagcaaacca aacaaaatca ggtgcctctc agctgaaaga tgcgatacca
     1321 agcatcacaa acagtgtaga tgttacattg aagtcaattg gacaagtggc aacaacgaca
     1381 ggttcgttaa tagatactgt aagaacagct attgaagatg gtaagctgac tgatgatgaa
     1441 aagaaaaaca tcagcaatgt attgaacggc tttattgacg gaatatctca acaacaaaat
     1501 gcaatcgata aactggtcga gttcctgact aaattgcaaa caagtgctgg aaatacgaaa
     1561 ttacagccaa ttattgatca gctaaatcaa ttaaaaccct tgctaagcaa tctgcaaaac
     1621 cgcctatctg ctttgaacac agctgttcaa aatgaagaca cgaaggctat tgatgaattg
     1681 ctagatcaaa ttgatgccgt tgttaataat atcaatggca tcattgctgg tatcaataca
     1741 ggtcaaatca gttccactgt cacttctcta ctagaccaag tcatcgatac gattgggact
     1801 gcacaaggcg cactgagcaa agcggaccag atcgactttg attcgcttct cagctcaacg
     1861 aaagcaactg taacgaatgc agtggcaatt ttagaaaaat atcaaaaaga aatgcctgca
     1921 attggacaag aaatccatga tgccaataca atgctgaatg gaaatatgga taccatcgtc
     1981 aatggcatca atgaaggggc agacctatac aacaatgaat taccgatcat tcagcaaaaa
     2041 ttaggattgg ccgctgattt tatccaaaat gattggccag cactaaaaaa tgaaatcacc
     2101 ggtacattga caacagtaaa tgaaaaaatg ccagaagtcg aaacagctgt gaacgctgct
     2161 gcggacttga tcaacaatga ctggccaaat atcaaagctg gtatccaaaa agcagcagac
     2221 gcgatccgaa aaggcgaaca agaagtcgat ctaggcgaag tcatcaaatt attgaaacta
     2281 gacgcaacaa aagaaagtga cttctttaca caacctgttg agctacaaac caatgcgctt
     2341 tatccaatcg caaataacgg ttcagctagc accccgtttt atactgcact atgtctgtgg
     2401 gtcggcgctg ttcttctttc aagtgttgct acgacagaat actatttagg aaagaaagac
     2461 cgaaacaatt ttacgaaacg agaacaattc gttgctcgta tgctgacatt tttgacaatg
     2521 gcgattgcgc aaagcttgat cgttacatta gggaatattt tcttactagg agtagatgtc
     2581 caaaatccgg tctacagcgt gtcatttgct gtcttgatcg ccttagcgtt catgatgatc
     2641 gtctatgtct tggctgcatt attcgataat gtcggtaaag ggatcgctat tatcatctta
     2701 gtactgtcga tctctggcgg aggcgggaac tatccgatcc aagtctcagg gaaattcttc
     2761 caaatgatca atccgttctt gccatttact catgcggtaa acttgttacg ggaatctgca
     2821 ggaggaattt attggccaaa tgctacgaaa gcaatctgga tcatgatcgg tttgtttgtt
     2881 gtctttggaa tcgtaggaac ggcaatctat ccgttcatcg aagaaaaaat gacgaaattc
     2941 aaagaagcgg cgcatgagag ccatattttt cattaaaaaa agaggttgtg aaatcagtgt
     3001 tttgacctga gtattagaga aaaaaatgga aaaatagttc ctcatatttt gccgtttttt
     3061 tgagctaata ccgagggtca gctgattgaa caccgtgtac caagaggttg taaaaaagct
     3121 gctctgcatc aagtaataag aacgaccaaa taaaaacagc ttcttatgtt tttaatttgg
     3181 ttgggcttat taccgcagat gcaagctttt aaacaccgtt tatcaagagg ttgtgacaac
     3241 tgcgctatgc ttcgagtagt aagaatgatg ggtagaaagc agccttacat ctctcttcca
     3301 aaagttaaga tatcaaaaag gatagaactt gattttctaa gtcaagttct atccttttat
     3361 tatagaattc aaagaaaaag cccataaaaa aatgttagat ttttgctgta ttaaagcgtt
     3421 tgaaaaagcg cttgatattt ttctgttttt ttccaattat tttcttaaag tttattgact
     3481 tttaaggtca gtgagcgtac tataacatct tgtttactat aaattttcga ctataaaact
     3541 tgaagtaaaa ttgtgtgtaa aggagtttag aaattggatt acttaaaaag gttgttagta
     3601 ggtaagcctt taaaatctgc tgaaaatgat gagcacaaat taacccgatt tgctgctttg
     3661 gcattattgt cgtctgatgc gttatcatct attgcatatg gtacggaaca aatcgttgtc
     3721 gttttagtcg ctttgtctgc tgcagccatc tggtattcgc tacccattgc cgcttttgtt
     3781 attattttat tgatttcatt aacgctatca tatcgtcaaa tcatccatgc ttatcctcat
     3841 ggcgggggag catatgtcgt cagcagtgag aatcttggaa agaatgctgg cttgatttcc
     3901 ggtggctctt tattgatcga ctacatgttg acagtagcag tatctgtctc agcaggtgct
     3961 gaagcgatca catcagcggt gccagcactc tacggtcatc aagtcgctat ttctgtgaca
     4021 atcgttttgc tactgatgat gctgaatctt cgtgggctga gggaatctgc atcgttccta
     4081 ttatttccgg tgtacacatt tatccttgtg atctcgttac tgatcgttgt tggtttatat
     4141 aatatcgtga caggtgctgt gccattgcaa gctactgcac ttccaggagc ggctgtgccg
     4201 ggtgtttcta tcgctttgat tttacgagca ttttcttctg gttcttcctc tttaactggg
     4261 gtggaggcga ttagtaatgc tgtaccattc tttaagaagc caagagcaaa aaatgccgct
     4321 gctacattga cgatgatggc attgatctta ggtttcttct tcgtggggat cacatttatc
     4381 aactattggt atgggatcgt tcctgaaaaa gaagtcactg ttttgtctca aattggtaaa
     4441 gctgtctttg gacatggtat tttgtattac gtccttcaat ttgctacagc gctgatttta
     4501 gcagttgctg ctaacaccgg tttttctgct tttccagttc ttgcatacaa tctggcaaaa
     4561 gataaattca tgcctcacat gtatcaagat cgtggggatc gactaggcta ttccaatggg
     4621 attatcacac tagcgctagg ttcgatcgta ttgctgttta ttttccatgg ctcaacagaa
     4681 cgcttgattc cgttatactc tatcggcgtg ttcattccat ttgctttatc tcaaacaggg
     4741 atggtcgtca aatggaaaaa agaaggaaaa agatggttaa gcaaatcgat tgccaatatt
     4801 acgggtgcgt tcatctcata tgcaatcatt gcgattttgt ttgtctatcg gttaggggac
     4861 atttggcctt tcttcatcat tatgccaatc gttatgttca ttttttataa gatccatgat
     4921 cattataaaa aagtagcaga acagcttcgt ttagaaaatg atgcaaaact acatgattat
     4981 gacgggaata ccgttcttgt attagtcggg aatgtgacac gggtaaatat cggtgcgttg
     5041 aattacgcac gttcgatcgg tgattatgtc gttgcgatgc atgtttcgct agacgaagat
     5101 gtcgaaaaag aaaaagaaat cgaagcagaa tttaaaaagc atttcccaga tgtccgtttt
     5161 tcaatcgtcc actcttctta tcgctcgatt gaaaatccaa ttatccgtta tgtagatatc
     5221 gtaagtaaaa atgcagcgaa acaaaactat acaactactg tgttgattcc tcaatttgta
     5281 ccaaacagac gctggcagaa tattctccat aaccaaacaa gtttgcggtt aagattgcga
     5341 ttgtcttggc gtgaaaatat cgttgtcagc acctatagct accatttgaa gaaataaacc
     5401 aagttttaaa atcaaaaaca gaccctctag ctcttaattg aagctagagg gtctgttttg
     5461 tcttattctc agctaaaggt gccctctata agccattaaa gatgggaaca gcgtatgaaa
     5521 tgttattatt cttcctattt agctgtaaaa cattttgatt ttgatgtctt gatcataatt
     5581 tccgaatcct ttgccaaata atgtacagcc tcctatattt tcagcttctt cctttacctc
     5641 gactcttaag tgccatgtgt cttcataagt aggaatctca gcctcgtcaa gtgaccattt
     5701 tttgttacca taactcagtg ccatttttaa ttggtgattc acaaacccaa cggtctaagc
     5761 aagtatcaaa tcgatagtga acatgaatga caggagacga aagaggcaat catttttgag
     5821 gcagtagaag acttgacggc caaaatcagt tttgaataac cgttacaaat ctttcgtgcg
     5881 tatcctcagg ttattcaaga tatcaatcaa gtcacactgc aacgaggaac cttcgtgtat
     5941 tggtgttgtt gcatagactc acaggctttg gattcgacct ttgctaaaga tgatacgttg
     6001 ttaccaatta ctttacgttt ggctaccgct agtcgtaaag gagtaagaag tgagcgtgga
     6061 caaagatcct ccctaaaaag gggtcttttt ctctatgtaa aaaagaggaa aagtttgctg
     6121 aatttaagtg ctttattgta tttttttgac gtacttaatc aagtacgcta taattatatc
     6181 agaaaaagga ggagagacaa tggctaatac aacgatgaat cctagcactg ctcgtaaaaa
     6241 tttttatcag ttattgaaag aggtaaatga aaatcatact gaaatcgaga ttattagtga
     6301 tcgtagtgga aacaatgctg tattgattgg gctggaagac tggagagcga ttcaagaaac
     6361 cctttttctt gagcaaacag gtactttaga taaagtacga gaccgcgaaa aagacgacag
     6421 tagctttacg aatatcgatg atattgattg ggaagctctt tgatgagtaa ctatacagtc
     6481 gcaatcaaaa actctgcgaa agtggattta agaaaaatca agcaaacgaa tttaaagaaa
     6541 caatttgaag aagtgattca gacattgaag gaagatccat atatgcccac gcagtctttt
     6601 gaaaagctga gacctacaca tgaaggcaga tactcacgca ggctgaatag acagcatcga
     6661 gtggtttaca aagttgatga ggaaaataaa gttgtagaaa tttattctgc ctggacacat
     6721 tatgagtgaa gtgagaagag ccaacctttt tagatagtta acaaagagtc tccccaaaaa
     6781 ggagactctt tttttgttct aaaaccaacc ttcttggatt tattgcatga tttctttaat
     6841 ttgttcctat atctccacct ttgaagggtc attgtttgag tttaggaatg gaatgttgac
     6901 gggtttatgt aaaacaagga ggaagaagtg tgacaaggaa aatcagtaaa attggagcat
     6961 cgttgttagt gttggcagtg gcaacgacca tcgattatcg ccaaagtgag gcgaagacaa
     7021 ttggaaatgt cgtttatcga gaaacagcca aaagtatgtt gaaaaaagca atggacaatc
     7081 aaccggagtc gtcttattgg ttcccagagg attcgttggc atggtctttt ggaaaagata
     7141 cagatacgaa atacaatacc agtgtcgttc ctttagcaga gcgggtatcc aaagcgacat
     7201 tgccgaacat gaatgtcact caaacggaag aagttaaagt cgtggcgctt tcggccatga
     7261 atagcagtat caacggcaat gcatcacgag ggatcgatat cttttacgcc aatgtctttt
     7321 cttattggca atatatcgat caattggttt acgggggtgc tcttctggtg aaggatcatc
     7381 gtatcgccaa gtctagttgc gacggatgca gtccataaga atcgtgtacc ggttttagga
     7441 accgcaaacc tcccatggcg agaagctgga atggctagat atgtttttag ccaaggatga
     7501 ggatggcaag ttcccgattg tataaaagat gatcgaagtt accgatacgg cagtcaccag
     7561 ttttgatgat gcagctgaag gcatcgaaca atcgaggact gatggaaaag gcttaaatga
     7621 aagccacgcc aaaggatgta agaattgatg cccaattcaa agaacaagca gacgagtatt
     7681 tagagatcat gtggtatgaa ttccatgacg agtgatggga aaatggattg gcaaaatgcc
     7741 ttagcggata agaaaaaaac ttagtcggtg gatgcagata tgaacccgtt ggcagtatga
     7801 agttggagta tacggaatgg ataaatagct aagaaaagca actctttgcc cataaggagg
     7861 gaagaatcag agtggattat tgccttaaaa atgacagaac taaaaaagcc tttgcgacca
     7921 acaaatggtc gcagaagctt ttgtttttta caataacatc agcgtgatgt acggctggct
     7981 ggatctagcc gtctttccaa aatacctaaa agccagtccg tgatgatcgc cattaatgca
     8041 gttggcaatg cacctgctaa aataatcgat gtgccctctg ttgcattggt tcctcggata
     8101 atgatatctc caagaccacc agcaccgacg aatgcaccga tcgcggttat cccgatggca
     8161 acaactaacg cgtttcttat gcctgccatg ataacagaaa cagagagtgg taactctacc
     8221 atgaatagtc gttgtctagc agtcatcccc atgcctttgc cgacatcaag gatgtttttg
     8281 tctacttgga tcatcccggt atacgtattc ttgatgatcg gtaagaggga atacaaaaat
     8341 acagtaacaa tcacgacatt gactcccagg cctaatccaa tgattaaaat ggagatcatg
     8401 gctagagaag ggatcgtctg gatgatattc gccaaacgaa tcacccagtt tgctaattta
     8461 cgtctcctag aaatcatgat cccaaccgga atcccgacaa ctgctgcaaa gaggacacca
     8521 tagatagaaa tcaggaagtg acggataaat tgtgcaaaga tataggagcc attttcctgg
     8581 aaataataga aaaattgttg aaagacattc attttatcca attgcttcac ttcccttcaa
     8641 aatagtggtt ttcttgtaag aaacgttgtg ctactactga tggttccact agattattat
     8701 ctgcttcgta gttcaattcc tgcatttttt ccgtactgat ggtatgacct aacttattta
     8761 aggcttcttt taaatgaggc gtatcagcta atatcttgtt gtctacaact ggagcagcat
     8821 catatggtgg gaagaattga cgatcatctt ccaacatgac tagatcataa ctggcgatcc
     8881 gtccatccgt agagtagcca agaacaatat ccatttttcc tgcttcgact gcgtcataaa
     8941 caagtccaat ctgcataggt aatatcgaat tgaaggagaa gccataagtt tcttggaagc
     9001 cgtcataacc gtctccttta cgattgatcc aagaagtatc tacaccagct actaattcat
     9061 ccgctacatt tttcagatcg ctgactttag atagatggta tttttgtgcg gtgtcttttc
     9121 gaacaagaaa gacatacgta ttttcaaaac tataggacgg gaaccacgtt tgaccgtatc
     9181 ttttctcaaa ttcatctttc acggtagcaa acgctttttt cggatctttt tcaggaggaa
     9241 ggttcaatgt agtcgttaaa tctgttcctg tgtagcgagc cgctgaaatt gaagcatctc
     9301 cgttcatcat cgcttgatgg ttgatagttg tagtagccag attgttgatg attgcggtat
     9361 ttttatctgt ataatgttca accattccgg caactagact tgctaagatc tgtgcttctg
     9421 aagtgattcc acctgtgatc gaaatggtgt cttcatctgt attgctggca agaccaggga
     9481 aggaacaact cgcaagtata agaagagaga aaagaaccat ggtcattttt agtatacgtt
     9541 tcattcttca ttctcctctc taagtgcgat cggtgtcagc cggtgttcca agagacctag
     9601 aagccaatca gccaatagtg ctaaaattgt tacagggatc gtacctgcca aaatgagatc
     9661 tggttggtaa ttattcagtc cactaaaaat caaatcgcct aaaccaccag cgccgatata
     9721 agaggcaagt gtagcccatg caatcacgta aacggcagct aaacgaatac cagccatgat
     9781 cgttggcata gcaatgggca attcaacgga aaaaatggat tggaactcag tcatccccat
     9841 gccttttgcc acatcccgat aattccaatc gacatttttc atcccaatgt acgtattacg
     9901 taaaatcggc aataatgaat aaataaagag tgcgataatc gcaggtaatt tacctacgcc
     9961 aaatagcggg atcatcaatg ccaataaagc aagagaaggg attgtctgca aggcactggt
    10021 taagccaatc acaatcgtgg cagcttttgg aaaacgtgtc agtaagacac caagcggtac
    10081 agcaatgatg ataccaatga tcaaagcaag tcctgaaata taaagatgtt caaaaccttt
    10141 tgagagaatt tctgaaccgt gctgatgaaa aaactgctgc ataggtcaga cctccttaga
    10201 ttcttcagga gtagctgact ctgcaacatc agtagttgcg ggatcttctc cccaaatcac
    10261 atcgtagacg atatccacta atgatgcacg agtcaggata cctacgactt tgttctgttc
    10321 atcaacgacc ggcacgtatt tcagcccgcg ttttaaaatg cgttgcaagg tatcacgaag
    10381 ataggcggat tcttttacgt aaaaaacatg tttattcaaa atgtcgccga cactggatac
    10441 ttttcctcgt tttttattca acgtttctac atcgataaat ccttttaata cattcgaatt
    10501 atcggtaact aacaacgtat ctacacgttt ttccctcatc agtttgattg cttcttgtaa
    10561 tgatttttcc ggtgtaatcg tgattgctgt atttagcata acttcgccga ctcttgtcgc
    10621 atttggcttg gcttgtagta ggcgatcttc tccaatcaac tcttctacga aatcgtttgc
    10681 aggatgacgt aaaatatttt ctggtgtatc aaattggatc acacggcctt cactcatgat
    10741 ggcgatacgg cttgctagtt taatcgcttc atccatgtca tgggttacaa atacgatcgt
    10801 ttttcccaaa cgctcttgta aatctttgac aaggtcttgc aaggaatcac gagtgattgg
    10861 atcaagcgca ccgaatggct cgtccatcaa gatgatatct tgattcgctg caagggcacg
    10921 aacgactcca atacgctgct gctgaccacc agataattca tgtgggtagc gatctaacat
    10981 ctcacggggc aattccacaa gatcaatcat tttttcagct gttttattcc gttcttcttg
    11041 actgactttc aacaattttt ggacaagagt gatattttca cggatcgtca tgtgaggcat
    11101 caatccaata ctttggataa catagccgat ctgacgtcgc aattccactg gatcaatttc
    11161 ttggatggat tttccatcaa tcaaaatttt cccttgtgtt ggatcagtca ttcggttgat
    11221 catccgcatc gtcgtcgttt ttccgcttcc gctcgtaccg ataaaacaaa tgaactcacc
    11281 cttttcaaat gaaagattga cgtcctctac agcgactttt ccacctttat aaatttttga
    11341 cacattttga aattcaatca tggtgccacc tctttttcta ttttcaatga agaactatct
    11401 attactagta tgcctaaaac aatgaaaaaa tacaaatagg gtagtttttt tatttaggaa
    11461 aacaaaaaat agaagtgaaa aagtgctgat tttgccaaca gaacgaaaaa catgatagaa
    11521 tgatgaagca catgatagaa agtgagtgac gatatgttaa caacagaaga ttttgatttt
    11581 gacttgccgg aagaattaat cgcacaaaca ccattaaaag accgcgatca ttctcgttta
    11641 cttgttttgg atcggaaaac tggagagatg gaagataaac atttccatga aatcattgaa
    11701 gagttacagc ctggtgatgc gctagttatg aataatacac gagttctgcc tgctcgttta
    11761 tatggagaaa aaccagatac tggtgcgcat ttagaagtcc ttcttctaac gaatacacaa
    11821 ggagatacgt gggagacatt gatcaaacct gcgaaacgtg ccaaagttgg gacaagaatc
    11881 tcttttggtg atggcagatt gcaagcagta gtgaaagaag aattggaaca cggcggacgg
    11941 atcattgact ttgagtatga aggaattttt ctagagattc ttgaatcatt gggagaaatg
    12001 ccattgcctc catatatcaa agaacgtctg gaagacccag atcgctatca aactgtctat
    12061 gcgaaagaaa atggttctgc tgcagcgcca acagctggat tgcattttac gaaagaatta
    12121 cttgatgaga tcgaagcaaa gggtgttgaa ttagtctatc tgacactaca tgtagggtta
    12181 ggtacattcc gacctgtcag cgtagataaa attgaagaac accatatgca tagtgaattc
    12241 taccgtttga cggaagaagc ggctgagcga ttgaaccaag tacgtaaaaa tggcgggaaa
    12301 atcgtggctg tcggaacaac ttcaattcga acattagaaa cgattggaac gaaatttaat
    12361 ggagaaatca aagcagacag cggatggact gatattttca ttacgcctgg ctatgaattc
    12421 aaagtggtag aagctttctc aactaatttc catttgccaa aatcgacttt agtcatgtta
    12481 gttagtgcct ttgcaggaag agaattgact ttatctgctt accagcatgc gattgaagag
    12541 cgctatcgtt tctttagttt tggagatgcg atgttcgtga aataaggtta tgaaaagccg
    12601 ctagttttga ataacaaaaa agctttacaa agattttatg acaaagaaaa agccgagctc
    12661 tcgataagct cggctttctt gtacctcgga aggattcaaa taaacagaaa tgattgtaac
    12721 tttccatcat acatgcaaag cacggtgttg aatttcttca tgttaaacaa aaattttgtt
    12781 ttcgttacat cttcaacatt tgaaacatct cttgttccta ttcaattttc agacgtttac
    12841 gttattcaat tgttacgtat cgcattactt cttgtttcgg atacttcgtg atccaaacat
    12901 ccatacgcat tcactattta taggtttgta ctctacacgc atgtctgggt gaaacctgta
    12961 cttctaacaa ctttgtttgc ttacactgtt ctatcgagaa attcttctgt ctatttgatg
    13021 gtatcctcct tccttacatc tatataatag catcctttgg tagcgtttgc aaatataagc
    13081 actttaaatt ttacgctttc ttaacaaata atcgataagc cctttatttg aagtaaataa
    13141 aaaaaggatg ttatagtaaa ataagtacct ttttttgtac gaagatttta aaggaggaat
    13201 aggatggaag aatacaagca gacaatagaa gaagtaaaga aggtaacaga agcagatcca
    13261 gatactgggt tgtcttcaca agaagtggat gaacggcgaa aaaagcaggg attgaataaa
    13321 ttcgatgagg cgccgaaaga atcgatgatc aaaaaattct ttcgaagctt gtccgatttt
    13381 acaacgatca tccttcttgt agcagcggtg atttcctttt ataccgcatt tgctacagaa
    13441 catggggatt tatttgaagg actgctgatc atcgctatcg ttgtgatcaa ctcggtattg
    13501 gcgattgtcc aagaaggaaa cgcagaaaaa gcactggaat ccttgcaaga tatgaataaa
    13561 caaacggcaa ctgtcatacg agacggcaaa gtgaccactg ttgagtcaga gcaactggta
    13621 gtgggtgata ttcttgtttt agagtccggt gatgctattt ctgccgatgc ccgtttgatc
    13681 gaagcatcac aattacgtgt agaagaatcg gcgttgactg gagaaagtga agcagttgaa
    13741 aaagatgcag catttattgc tgaagaagat gagtcattag gtgaccaact taatatggta
    13801 tttaaaggat gtacggttgc tgcaggtcgt gggaaagcga tcgtcactgc cattggcatg
    13861 gctacagaaa tgggtaagat cgcggatctt ttgaacgaaa atactatgca aaaaacacct
    13921 ttacaggtcc gattgaatca gctaggcaaa cgaatcagta tgatcgcttt agcggctgcg
    13981 gctttagtat ttgtgattgg tgaactgcaa ggagaaccac tccttgagat gttcatgact
    14041 tcgatttcct tggctgttgc agctgtaccg gaaaccttga ctgttattgt tacgttgaca
    14101 cttgcatacg gtgtgcagaa gatggcgagg aaacatgcga tcatccgaca gttgccagca
    14161 gttgaaacgc taggaaccgc taatgttatc tgctcagaca aaacaggaac gctgacacaa
    14221 aatgaaatgc gcgtccgccg ggtatggagc aaagaagatg aagtaacgaa tatcgaagat
    14281 tcaatgacga acagcgcgat ggaaatattg aaaatggctg cactatgtac agatgtgacg
    14341 gtcgagcaag aagatgacga ccttgttatc aaaggtaatc caacagaagt agccatcgta
    14401 agggctgttg aagagaatta tcatacaaag gcagaattag aagaaaaata tccacgagta
    14461 aacgagcttc cttttgattc tgaacgaaaa atgatgacaa cagttcacca aatgggcgaa
    14521 aaatacatct caatcaccaa aggagcattc gatgttcttg ctccgcgctt tagttcaggc
    14581 gatgtagaac aagcggcgat cgttaatgat cgctttggta aacgggcact gagagtgatt
    14641 gctgttggtt atgctactta tgacgaagaa ccgcaagata tttcttctga agcactggaa
    14701 aaagacttac gtttgattgg tctgatcgga atgatcgatc caccacgtcc agaaagtaaa
    14761 ggagcaatca agcgagcgaa aaaggctggc atcaaaacag tcatgatcac tggagatcat
    14821 gtggtgactg ccagtgccat agcgaaagaa ttaggtatct tgaatgatcc gtcagaagca
    14881 ctttcaggat cagaactgca tcaattatca gacgaagaat tagatgcgcg tgtcaaagct
    14941 ctctctgtct atgctcgggt aacgccagaa gacaagatcc ggatcgtcaa atcttggcaa
    15001 cgtacgggtg cagtcgtagc gatgactggg gatggtgtga atgatgcacc agcgttgaaa
    15061 gcaagtaacg ttgggtgtgc catggggatc acaggaacag atgtggcgca aagtgccgca
    15121 gatatgattt tgacggatga caactttgcg acgatcgtag acgctgtcag tcaaggacgt
    15181 tcggtttatc agaatattcg taaagctatc aactttttgc taagctgtaa tatttcggag
    15241 atctttattg ttttgattgc gatgctgtta ggatggggag caccatttac agctgtgcaa
    15301 ctactgtttg tcaatgtagt tgctgatgga cttccaggat ttgctttagg aagagagcca
    15361 gcggaacacg gcatcatgga tcagccaccc attccgaaaa atgaagggat ctttgctcga
    15421 ggtttattgc aaaaaattgc cataaatgca ggggtcttca ccatcgtaac cttgtttggt
    15481 tattatttag gtagttatgt cgatactatc agcccatggg tagacgcaag tcagcatgtc
    15541 ggtcaaactg ttgcattctt gatcttagct tattcatcga ttctgcatgt gttcaatgta
    15601 cgcagcagcc aatcgatctt caaagtcaat ttagcgacca ataaagcttt ggtagaaatg
    15661 gcgttactgg cattagctat tacaacggct gttgctttgc tgccatttac gcaagaacta
    15721 ttcggcctag tacacatcag tttgaatcac tggttcttgg tagggatttt atctatcgta
    15781 ccaatcgcgg tgaatgaact gatcaagttc catcgattac cggaagcaga agaagaggaa
    15841 tgattaactg tttctaagtt gattttcttt ttactggaac tatacaaaaa ggaggtgtga
    15901 catgaatgtt gtcacacctc ctttttgcta atgggctgaa tgtttctttt actgcctctc
    15961 gataaacggt gttctcctgc caccccctcg gtcacaagtc acactctctt ttaaatatct
    16021 tttggcaatt gttcataaaa tgctgctttt gttgcagaaa tgtatgggct gagccataga
    16081 taaccaatac cagcagttgc taatgctaag atatgccagc cgataaatga aagatctaac
    16141 cagaaaagtt gtcctttata accatccatc aattttcgac ttgcagtgat cgtatccagt
    16201 actttaggtg tttgtcctgt ttcttggacg atatcgtagt aaataaaata gctttgagaa
    16261 taagaatagc ttttaatgat tccaggaata actaatagca aaccccataa tgttgtaaag
    16321 atcgagatca gcacagctaa gctgatcact ccgccgataa acaaaccttt gaatccacga
    16381 aatgcatctt tgaatggttc gatctgatct ttttgtccac gtaaaatatc aagataagtc
    16441 caagaaatac cgctcatgaa caaagcgcta atgatgctgg aaataattcc gccaccgcct
    16501 cctgaactac cgcctcctga agttgtcgct tctgagacgc tcgctgatcc atcatatatg
    16561 gtggtggcaa acaagataat aggaatcata ataagaagaa tagtagcaat accaatcaat
    16621 gtagggatta gatttagtaa aacagcttgt ccccaacgtc cttttagact ctcttttgct
    16681 tggcgtttta attctgcaga agattgcatg atttttccct ccttttcttt aataacacca
    16741 atataccatg tatcaaaaaa tatttctgtc ataatgtttt tctttttttt gttattcata
    16801 aaaaaaggat aaaaaaaaga atattaaata aagtagaagg aagccgaggg aacttgcaaa
    16861 aagcacttga aacgattatg atggtgggag ttgtcaagat attttatata gaaagtaggg
    16921 aaatgaatga cgcaacctgc cattcgctat cgtttaatca agaaagaaaa acatactggc
    16981 gcgcgtttag gggaattgat cacgccacac ggaactttcc ccacaccaat gttcatgcca
    17041 gtaggaacat tagctacagt aaaaacaatg tccccagaag agttgaaaga aatgggtgct
    17101 ggcgttattt taagtaatac atatcattta tggctacgcc ctggtgaaga cttagtagaa
    17161 gaagctggcg gactgcataa atttatgaac tgggatcaac cgattttaac agattcaggc
    17221 ggctttcaag ttttttcatt aagtgatatg cggaatatcg aagaagaagg cgtgcatttc
    17281 aaaaatcact tgaacggttc gcgtatgttt ttatcaccag aaaaagcaat caacatccaa
    17341 aataaattgg gctctgatat catgatgagt tttgacgaat gtccaccatt cgatgaaagt
    17401 tacgactacg tgaaaaaatc agtagaacgt acgtctcgat gggcagaacg aggattgaaa
    17461 gctcatgcta atccagaccg tcaaggatta tttggaatca tccaaggagc agggtttgaa
    17521 gatcttcgtc gtcaaagcgc gaaagatcta gtcgggatgg atttccctgg ttactcaatc
    17581 ggcgggttat ctgtcgggga acctaaatca gaaatgaatc gtgtattaga attcactaca
    17641 ccgttgatcc ccgaaaacaa accacgttac ttgatgggtg tcggtgctgc tgattcattg
    17701 atcgatggtg ttatccgtgg agtagacatg tttgactgtg tcttgcctac tcgaatcgct
    17761 cgaaatggca catgtatgac ttctcaaggc cgtctagtag tgaaaaatgc gaaatatgcc
    17821 catgatttcc gtccaattga tgagaaatgt gattgttata catgtaaaaa ctatacacgt
    17881 gcctatatcc gccatttgat caaatgtgat gaaacatttg ggattcgttt gacttcttac
    17941 cacaatttat acttcttgtt gaatctaatg aaacaagtac gtcaagcaat catggatgat
    18001 aatttgcttg aatttagaga agcctttttt gaagaatacg ggttcaataa agaaaacgca
    18061 aaaagcttct aaaggaaaat cttttaaaaa ttaagtaaaa aactagtgtt atcagcattt
    18121 ttctgctaaa gtgttttttg tagatacaga attagtgagg tgaaaaaaat gggaacatta
    18181 ccaatgcttg tgatgtttat cgcgctatta gcaatgtggt tttttatgtc acgttcgcaa
    18241 aagaaacaac agcaagaacg tcaaaacttg ttagatagca tgaaaccagg tgattcagtc
    18301 gtaacgatcg gcggattaca tggagttgtt tcagaaatcg acaatgacaa acggacagtt
    18361 gttttagact gcgaagggat cttccttgag tacgatcgtg cagcaatcaa aacggtcaaa
    18421 ccagggactg tcgtaacaaa tgatgcaacg actactgttg aaacaacaga agaaccagct
    18481 gagacagttg aagtaaaaac tgaaacaaca gaaacgctgg aagagacaga agagaaggaa
    18541 gaaaaataag tatcaaagct tatttaaaga cgaggtaggg cagaaagatg tcctacctca
    18601 ttttttattc aaaaaagaaa gagataagag catgggatat attgctcaat attgaataat
    18661 cgggtgtgtc atataaataa taataaataa ctttatatat ttttctatac aaacacctgt
    18721 aaataaagca attttatatt taaaaataac tgtgaaaaaa atcacaaaaa agtgtttaca
    18781 tagaaaaata aatattgtat gatatacatg tgaattattt aacaaataac catgacccat
    18841 taggaggata atgatggcta aagtaataga aaaagaaaaa acgaagacaa ttgacgtaca
    18901 agcaatgatt gatgatttgg cagaaaaagc aaatgcagca ttgaaagaaa tggaaagctt
    18961 tgatcaagaa aaagtagacc atatcgtgca tgaaatggca atggcagctt tggatcaaca
    19021 tatgccatta gcgaagatgg ctgtagagga aacaggccgc ggtgtttacg aagataaagc
    19081 aatcaaaaat atgtatgcgt ccgaatatat ttggcataat atcaaacatg ataaaacagt
    19141 tggtgtgatc aatgaagatg tccaaaaagg gttgattgaa attgctgaac cagtcggtgt
    19201 ggtttgcgga gtaactccaa caacgaatcc gacttccaca acgattttca aatcaatgat
    19261 cgcgttgaaa acaagaaatc caatcgtttt tgctttccat ccaagtgccc aaaaatcttc
    19321 tgcagaagca gcacgagtgg tacgtgatgc tgctatcgct gccggagctc ctgaaaattg
    19381 tatccaatgg atcgaacatc cttcgatcga agcgacttct atgctaatga accatccagg
    19441 tatcgcgatc gtcttagcga caggtggagc tggaatggtc aagtctgctt actctacagg
    19501 aaaaccagct ttaggtgtag gtccaggtaa cgtaccagct tatattgaaa aaacagcaaa
    19561 aattaaacgt gcagtgaacg atttgatcgt ttcgaaaaca tttgataatg gaatgatttg
    19621 tgcttctgaa caagctgtga tcgtggataa agaaatctat gcagcagtaa aagcagaatt
    19681 ccaagcacac caagtttata tcgtaaaacc agatgaattg caaaaattag aagatgcagt
    19741 gatgaacgaa ggaaaatatg cggtcaatcc atcaatcgtt ggacattctg cgatggaaat
    19801 cgcaaaattg gcaggaatca gtgtaccaaa aggaacgaaa atgctgattg ctgaattgga
    19861 aggcgtaggc ccagattatc cgctatcaag agaaaaactt tctccagttc ttgccatgat
    19921 caaagctaac aacacggacc atgcgtttga tttgtgtgaa ggtatgttga atcttggagg
    19981 attaggacat acagcagtta tccattcaga aaacgaagag ttgcatgtca aattcggtct
    20041 tcgaatgaaa gcttgtcgta ttttagtgaa tacaccatct gcagaaggcg gcatcggtga
    20101 tatctataac gaaatgattc cgtcattgac tttgggatgc ggttcttatg gtaagaactc
    20161 tgtttctcgt aacgtctctg ctgtaaactt aatcaatgtc aaaactgtag cgaaacggag
    20221 aaataacatg caatggttca aattaccgcc aaaaatcttc tttgaaaaga actctctatt
    20281 gtacttagag aaaatggaaa acgttgaacg tgtcatgatc gtttgtgacc ctggtatggt
    20341 ccaattcggt tactgcgata cagttcgtga agtattggca cgacgtaaaa atgatgtgaa
    20401 gatcgaagtc ttctcagatg ttgaaccgaa cccatcgaca aataccgtat acgcaggaac
    20461 gaaactgatg gcagacttca agccagatac agtaatcgct cttggaggcg gttctgcaat
    20521 ggacgcagct aagggaatgt ggatgttcta tgaacaccca gacacatctt tctttggtgc
    20581 aaaacaaaaa ttcttggata tccgtaaacg gacatataag atcgacaaac cagaaaaaac
    20641 acaattcgta tgtatcccga caacatcagg gactggttct gaagtaacgc catttgcggt
    20701 catcactgac agtgaaacac atgtgaaata tccgttagca gattatgcat tgacgccaga
    20761 tgtcgcaatc gtcgatcctc aattcgtcat gtcagttcca gcatctgtca cagcagatac
    20821 cgggatggat gtgttaacac atgcaatcga atcctatgtt tcagtcatgg cttcagatta
    20881 tacacgtgga ttgagcctac aagcaatcaa gttagtcttt gactatttag aaaaatcagt
    20941 aaaaacacct gatatggaat cacgtgaaaa aatgcacaac gcttctacaa tggctggtat
    21001 ggcgtttgct aacgcattcc taggtatttg tcactcagtc gcacataaaa tcggcgggga
    21061 atacgggatc cctcatggac ggacaaatgc gattctttta ccgcatatta tccgctataa
    21121 tgcaaaagat ccacaaaaac atgcgatgtt ccctaaatat gactatttcc gtgcagatac
    21181 agattacgca gatatcgcaa aattcttagg tctgaaaggc gaaacaacag aggaattagt
    21241 cgaagcttta gcaacggctg tttatgaatt aggaaaatct gtcggcatca acatgagtct
    21301 aaaagctcaa ggagtcacac aagaaacact agatacaacc gtagaccgta tggctgaatt
    21361 agcttacgaa gaccaatgta cgacagctaa tccgaaagaa ccattgatct ctgaattgaa
    21421 acagatcatc attgatgctt acaacggata agcaaaagaa agcgtagaaa aaagataaga
    21481 aaatagatga aaaagaactt ggaaaacgtt gtttctccaa gttctttttt taatttccaa
    21541 gtaactgaaa gttacttttg ttatttaaca tttaggtcaa ttttagacag tttttgttca
    21601 aaaagcgata tttatagtgg acggtattac tgctatatta aggatataaa atgaaagcgt
    21661 ttaaaaaggt ggcgtaaaga tgattattaa agaagagatt tttgtaccta aagaattagt
    21721 gaaagaaaaa attgacctgc tcgttgaaaa cctgacaacg atcaaagacg ataatggtga
    21781 atttttgttg gactttgatg gactgaaagt agatgacaaa agctggacag tgtggaattg
    21841 gccacaaggt gtcggtctat atggtattta taaaaattac cagatgacaa aaaatcctcg
    21901 cgcttatcaa gtggtcaatg aatggtttga ggatcgtatg gaagaaggcg ctccgcccaa
    21961 aaacgtcaat acaatggcac cgctattgac tatggcttac ttgtatgaag acacgaaaga
    22021 ttcacgctat ttgccttatt tagaacaatg ggcagaatgg gtcatgaatg acatgcctcg
    22081 gacaaatgaa gacgggttac agcatgccac atatggaccg gaaaataaga atcagttgtg
    22141 ggacgataca ttgatgatga cagtgcttcc tttggcaaaa atcgggaaac tgttgaatcg
    22201 cccagaatat ttagaagaag ctcgttacca gtttatgatc catatcaaat acttgatgga
    22261 taaacgaaca ggattgtggt atcacggctg gacgtttgaa ggaaatcata actatgccga
    22321 agcattttgg gctcgtggaa actgctggtt gacgattgct attccagaga tcattgaaat
    22381 cttagaatta tcgaaagaag atgcattgcg caaattattg atcgaaacac ttgaagcaca
    22441 agtgcgtgca ttaaaaactt atcagtctga aagcggttta tggcatacgt tgttggatga
    22501 tccttcttct tacgtagaat cttctgcaac tgcaggattt gcttacggta ttttgaaagc
    22561 ggtccataaa cgttatttac cacaagaata taaagaagtc gcttatcgtg ctatccaagg
    22621 attgcttgaa caaatcgatg aaaaaggaga agttcaaaac gtttcaatcg ggacaggaat
    22681 gggggacagt ttagattttt atcggaatat cggaatcaca gcaatgcctt atggacagtc
    22741 gttaacggtg ctttgtttga cagaattgct cgtctcttat tattaaaaga aaggaaaccc
    22801 atatgaaaaa aacatcactg ctgtttgcag caagttcgct tagcctaggt ctgctaggtc
    22861 taagcggctg cggaaataat tctagtgtgg caactgtcgc agatgcagat gtaaatgaga
    22921 tagttacctt gcgctttgct tatgcaagca acagccagcc agtaattgat gcaatgaatg
    22981 aatttgggcg tttagtaaaa gaaaaaacag atggttccgt acagatccag tatttcccag
    23041 atggacaact gggtggagaa cgtgaactga tcgaattgac acagtcagga gcagtagatt
    23101 tcacaaaagt aagtgcctca gcgttggaaa gtttctctaa agattattcg attttttcag
    23161 ttccttatct attcaatgat gaagaacatt tctttaaggt gatggataat gaagaaataa
    23221 tggagccaat ctaccaatcg accaaaaatt taggatttac cggattgacc tattatgatt
    23281 ctggtcagcg cagcttttat atggtagacg gtccgatcca tacgccggaa gatttgaagg
    23341 gaaagaagat ccgtgtgatg caaagcgaaa ccgctatcaa aatggtagag cttttaggcg
    23401 gttcgcccgt acctatggga agtgatgaag tgtacacgtc tcttcaatcc aatttgatca
    23461 acggatcaga aaacaatgaa tttgtcttgt atacagctgg acatggtggt gtggctaaat
    23521 actattccta tgatgagcat acacgtgtgc cagatatcat catcatgaat gatgccatca
    23581 aagaacagct aactgatgag caggaaaaag ccattgaaga agcagcgaaa gaatcaacgg
    23641 tttttgaaat tgaagctttt gcaaaagcaa ttgaagaaga aaaagcttta gcacaagaag
    23701 aacatggagt ccaattcaat gatgtagata atacaccgtt tcgagaggca gttgccccac
    23761 ttcatgatat attcaaaaac gatcctacct acaaagacct ttatcaaaag atccaagagc
    23821 aaggagcgta gctgtgatga aaatgatttt aaacaaggta ttagagatcc tgggagctgt
    23881 tttgcttgtt gggatggtcg ctgttgtttt atggcaagta ttttccagaa cggttttagg
    23941 aaatccaaat acggtcacgg aagagcttgt tcgattcggt cttgtctggt ttgccatgtt
    24001 gtcttccgcg tatgttgtgg gacaaaaagc acatttagcc gtaacggttt taagtgaaaa
    24061 attaactggc aaaaaaagta aaattttaga agttgttgtt caaagtttat ttctggtatt
    24121 tgctgcgatg atcatggtgt atggcggatg gaatgctgtc acattgacaa tggggcagat
    24181 ctcgccttct ttaagtattc ctatgggata tgtttatctg tcagtacctg tatcgggggt
    24241 actcatcatt atctatagcc tgatcaatct aatcgataca ttaagcaaca agccagcaat
    24301 tcaagaaaaa atataaaaat ggaggaataa aatggcaata gaagccggat tagtgttatt
    24361 ttttgtattt gttgtgttgc ttgtattagg gatgccgatt gctataagta ttgcattctc
    24421 atcgatgatg actttattgt tagttatacc gtttgatgtt tctgctttta gttcagctca
    24481 aaaaatggtg ggaagtatca atagtttttc gctgatcgct attccgtttt tcgttctctc
    24541 gggcattatc atgaataatg gagggatagc taagaaacta gtggattttg cgatgttgtt
    24601 cgtcgggcgc gtgccggggg ctttggctca tacgaatgtg ttagggaatg ctttatttgg
    24661 ttccatctct agctcggcca ttgccgcttc taccgcgatt ggcggagtgt tgatccctca
    24721 gcaagtggaa gaaggatatg accggaagtt tgcgacagcg gtcaatatcg cgtcagctcc
    24781 gacaggtatg gtcattcctc caagtactgc ttttatcatg tattcacttg ttgctggtgg
    24841 tgcttcgatc tctgctttgt ttatgggagg ttatctcgta ggcgctttgt gggcgctagg
    24901 aatcatgcta gtagcattca tcgttgctaa aaagaataaa taccctgttg tcgaaaaagg
    24961 acagatggct catgctggaa aaataataaa agaggcgatt cctagccttc tattgattgt
    25021 catcattatc ggtggtatct tgacggggat atttacagca gtagaagctt cagcgatagc
    25081 tgtcatttac tccctgttga ttgctatgtt ttactataaa acggtaaaac taaaagatat
    25141 acctaatatg ttgtttcaat cagtagcaat gtcagggacg atcatgttct tattggctac
    25201 ttcttccatg atgtctttcg ccatggcgtt taccggtatt cctcaggcaa ttagttcgtt
    25261 gattatgggc gtaacggata ataaatttat tattctgtta ttagtcaatc ttgtgttatt
    25321 actggtgggt atgtttatgg atgtggcgcc tgctatcttg atttttacac caatattttt
    25381 accaatcgtc acaagtgtag gggttgatcc tgtacattac gggttgtttt caatcatgaa
    25441 tctgtgtgta ggctccatca cgccgccagt aggtacagga ctatatgtcg gtgcaagtgt
    25501 aggaggcgta aaagcagagg caatgctgaa accgcttctt cctttttatg gggttatctt
    25561 agtcgtcttg tttttgatta cttacttccc gcaaatcgtc atgtggctac ccaatatgct
    25621 gagttaggag aatagttatg ttgaacatta caattagagg acatgatctt tccgaagtcc
    25681 aaacgataga agatcttgca gaaaaaacaa aagaacaagg gattcataca ctgcagctcg
    25741 ccttaggtct ttcgtttcct gaaatacctt ctggcgcaaa tgagatcaat tctgggatgg
    25801 gaaattatgt caaacgagtt ttagaaaagc aagaagtgac cgtaggaatt ttaagttgct
    25861 atatcaatat gattcatcca gatttaacga tacgacaaga gttgctgaca aagttcgagc
    25921 agtatcttcg ctatgcttct tcctttggtg cgtcgatggt tgcttcggaa actggatgtg
    25981 ttttgccgga aatccaatat acggaagaaa atttcacaga tgaagcattt gctgaagctg
    26041 tgtccgttat ccgtcgcttg gtaaaagctg gagaaaaata ccaaatgatg gtggggatag
    26101 aaccaggttt gaatcatccg gtgtattctt tagcaagggt agagcagctg atccaagcag
    26161 tagattcgga ctatctagga atcattttag atccgaccaa tttgatcaca agtagtgatt
    26221 atcaagaaca agtccaattg gtagaagaag cttttgaacg ttttggtgaa aagatatgtg
    26281 cggttcattt gaaagatttt cgtgtcgaac aggaaaaaat cgtgccggta aatttgggag
    26341 acggtgtgat cgagtacaca aaaatcaaag aaatcatcat gaagaatcgt ccgtacctgt
    26401 atgtggttct tgaagaaaca aaagatgatg ggattcgtta tggaagaagt cttcttgaat
    26461 aatgtaaaga agacgttggt taactaatat ttagaatgga gggggcgggg ctagaagatg
    26521 ccacgtctct tcttttttat aagagtatat aaaagcagct ctttttgatt acaaaaaacg
    26581 tggtataata aaattgtgca taagtgaaca aaaggaggtg cggcacatgc tccaaaagaa
    26641 aatcagcggt gaacaagtca atacgatggt ttctgatcag tatgaaatct atcatgtcaa
    26701 agatgtcgtc agccaaaata aaacgatcta tcatcatcat gatttctatg agatccatgc
    26761 tacattaaag ggagaagcga ctttttattt gaacggacga caatttacga tcaaagcagg
    26821 aagtgtgctg ctcatccatt ccaatgattt gcatcgtatc gtccgccaat cttcagatgt
    26881 attcgagcgt gtctatatct ttgtcacccc ttcttttcta gaaagtcgtt ccacgccatg
    26941 gtctaatcta tcggcttgtt ttcaaccaat gggagagaag aggagtcgtg tattgcaaat
    27001 agaacaggaa gtattgaagc aaaaattatc atttatcgac catcctcctg ataaaaaaat
    27061 ttacggtgaa gacataaaat atgaacaagc tctggtagaa tacttgattt ttctcaatca
    27121 agttgtacaa aaagaagaga gtgcgcaaga acagaatgct gtagttccta atgagcgtgt
    27181 ggaacaaatg ttacaatata tctcaaaaaa tttatctcat ccgctttctt tagaggaaat
    27241 ggaaaagcag ttttttgtca gcaaatacca tatcacacga gaatttaaaa agtacactgg
    27301 atttacgttt catcagtatg taatgaagaa aaagctgatc tatgccaagc acttgttgcg
    27361 agaataccgc agctctagtg ctgtatatag taaaagtggt ttttcttctt atccgcattt
    27421 tctgaaagca ttcaagagag agttcggcat aacgcctaaa gaatttttga aacgtgatac
    27481 acagaaacag ttggttcatt atgaacattt tgaagatgaa caatgagaat gatcactaaa
    27541 aaataaccgc agtagctgct gttgtttaca tagaagcaga aactgcggtt attttatttt
    27601 tatctatata tttcaaaata ttttgaaaaa atgacgtaca tattatatga atcagctgaa
    27661 tttacgaaag tctctcggcg atagtcctgt ttttgctttg aacaggcggc taaagtaagc
    27721 aaaattctca atccctattt cttcggaaat cattcgaacc gtaaattctg tataccgaag
    27781 aaggtcttta gcgtaagaaa gttttttgct gatcaaataa tcgattggtg gagaaccgat
    27841 aaatttggaa aaatctttgc tcagctgata tttattcaat aagaattgtt gttctaattg
    27901 atcaagtgta atcgtttctt tgaaatgttc gtctaagtat tgcttcagtt tgtaaacgta
    27961 ggcgggaata tcctcttctt cgaaatctag ctgatacttt tgcaaaatca gttcattcag
    28021 caactcgtgg atagctacag aggactggaa atcggtacgg gcattcgtgc cttgttgcat
    28081 gtggatcaat tctgtgatca aatggtggat cttgttttct gatggatctc ctgttgaatg
    28141 gaaggtgtta tggccgtctt ttgtgaactc ctgataaaat gattggacgt tctgaccatt
    28201 caaatggatc caatccattt cccatggttc ttcgctgatc gtttgataac gctgatattc
    28261 ttggcagtca ataaaaaata agtctccagc ttttagctgt tgctgattat cattatatgt
    28321 aagcgaacct ttgccagaca aagtaaactt tatcaagtaa gaaggtaaat gtgctcgttc
    28381 agtatagtaa gggattggtg ctttgaagta accgatttct tggatataaa aaaataaatt
    28441 cctgcttctt tgtgtaggcg tcaagatcac tctttttgag tcttccgacc atgagtggtt
    28501 ggctttttcc cagtaatcca tgtaaacact cccaacttgt caagataatg caattatata
    28561 ccaaaatagt gtgaagcaaa atataagatt ctgtattatg atagcattgt caataaatga
    28621 taacgcttta ttaataaagt gctaatcaac aataaagtaa aagaggtgga gaaatgaatc
    28681 caacatttgt tgccatcgat atcggtgcat caagcggacg actaatgaaa agtacgaggg
    28741 atgcaaaagg ttctttgatc ttggaagaag tacaccggtt caaaaacggc tttcggaaca
    28801 aagacggtca cgatcgctgg aagatcgatt atcttatcaa agagattttg attggatttg
    28861 aaaaattaaa gcagacaggg attgatcgcc tttttgcggg gatcgataca tgggcagtcg
    28921 attactgtct gattgatgaa caaggggatc ggataaatga tccgattgct tatcgggaca
    28981 agcgtaccaa tggagcagta gaacagtttt ctgaaatcta ttctctagat aagttgtatg
    29041 agcagacagg gattcagatc cagcctttca ataccttgtt ccaattgttt gctgaagaaa
    29101 aagaaaattt ggcaaaagca cataaattgt tactgatacc tgattattta gggtatgtct
    29161 ttactggtaa catggtgacg gagaaaacta atgcatctac tatgcagctg ctgaatgcag
    29221 ggacaaaaga atgggaaata gaacttcttg aaaaaatcgg tgtctcgaag gaattatttc
    29281 caccattgac agagccggga acgatactag gagatctgca aaaagaacaa ttttcaaaat
    29341 atgatttgcc agatgtgacc tttatcacag tagcaagtca tgatacggcg tcagctgtat
    29401 taggaacgcc aggtcaagga gaacgttggg gttatatcag cagtgggaca tggtcgcttt
    29461 tagggattga aacaaagatc gccaatgttt ctcgcaatgc tttttctgaa aattatacga
    29521 atgaatgggg tgcccataac acggtgcgtt ttttgaaaaa catcatgggg atgtggttga
    29581 tccaggaagt tgcccgaatg caagagtacg agtacagtta tgcggaattg gcagagcttg
    29641 ctgcaaaaga aaaaccgctg cagcaagtga tggaaatcaa tgaccctcga tttttgaatc
    29701 ccccaaatat gatagaagag attcagcaat actgttttga aacagagcag ccagtccctg
    29761 tctcatcggg agctatcgct cgttgtgtgt atgataatct tgctctttgt tacgcacaag
    29821 agctgatcaa agtagaacac ttaactggaa cgaaattgga aaagctgcac atcgtagggg
    29881 gcggatcgaa taatcgtcta ctgaatcaat tgacagcaga ttatgccgga atcattgtag
    29941 aagcaggacc tagtgaagcc acagcaatcg ggaatgttgc catgcaaatg ctcagtacag
    30001 gtcagttttc gaatatagaa caagcaagaa atgtaatcaa acagtcgttt ccatgtgaaa
    30061 ctttttaccc agatgaaaat aagcaaaaca cgatggaatg gtacgtgaat aaaatcaaag
    30121 gagtaatcgt atgaacacga ttgaaagaaa ttacgaacaa gcaaaagaaa aatatgcaac
    30181 tatcggtgta gatacagatg ctgtactaga aaagatacag gacatcaaaa tttctatgca
    30241 ttgctggcaa ggagatgatg tcaaaggttt cttgacaccg gatggcgaat tgaccggtgg
    30301 aatcatggcg acagggaatt ttccaggagc cgcacgaaca cctgaagagc ttagacaaga
    30361 tctggagaag gcttattcgt tgataccggg caaacacaaa ttgaaccttc atgctatcta
    30421 tttggataca gaagaatctg tcgatctaaa tgaaattgag ccgaaacatt ttgaaaaatg
    30481 ggtagagtgg gcaaaaaaag aagaaatcgg tcttgacttc aatccgactt tcttttcgca
    30541 tccaatgatg aaagacggct ttacattggc tcatccagat aaagaagttc gtgaatactg
    30601 gatcgaacac gggaaaagaa gccgcaaaat cgcggagtac tttgggaaag aaactgggca
    30661 aacaagtatc aataactttt gggtacctga tgggatgaaa gataatccga ttgatcgttt
    30721 taccccacgg gaacgtttaa tggaatcact cgatgaaatt tttacagaag agttagatga
    30781 acggtatgta caagatgcag tagaaagcaa actatttggt ttaggcgctg aagcttatac
    30841 agtcggttcc catgaatttt atatgggtta cgggttgact cgtgacaaac ttgtttgtct
    30901 agatgctgga cattttcatc caacagaagt gatttccaat aaattatctt cattagcttt
    30961 attcagcaaa ggaatcatgt tgcatgtttc ccgtccagtg cgttgggatt cagaccatgt
    31021 ggttttgatg gatgatgaat tgcaggaaat cgctaaagaa ttagtccgta acgaattgtt
    31081 ggaaaaaaca aatatcggtc tggatttctt tgatgcaacg atcaaccgta tcgctgcttg
    31141 ggtcatcggt acgagaaata cacaaaaagc actgctgaaa gctatgctgg aaccagttga
    31201 gcgattgaaa gaaatggagc tggcttttga ctttacttca agaatggctt atacggaaga
    31261 attgaaagat ttcccgtatg ccgatgtatg gaattatttc tgttacaaaa atcaagttcc
    31321 agttggtttg gattggctgg aagaagtcca ggaatatcaa aaagacgtgt tggaattaag
    31381 aaaataggag tgtggctata ttgaaaaaaa tagatatttt acaagcgcct tatgtgaaag
    31441 aaatgatcga aacgacgtcg aatctttatc gcttgggatg ggatgaaaga aatggcggga
    31501 atatttctta tttactaaaa gaagaagaga tcacaccgtt cttggatcct gaaaaagtga
    31561 tccgccgcat ctcgatgatt tttgatgcca gccgtttagc aggatgctat tttattgtaa
    31621 ctggttcagg aagatatttt aaaaatgtag cgagcgaacc tgccgagaat cttggaatcg
    31681 ttcgagttgc tgaagatggc aaaacactgg aacttttgtg ggggctggaa aacgaatgcc
    31741 ttcctacaag cgagttgcca agtcatttca tgagtcatat cgcccgttta gaagtagacc
    31801 cagataatcg gatcgttatg cattgccatg ctagtcatct attagcgatg agttttacgc
    31861 atgaattaga cgaacgttcc tttagtcgta cgctatggca aatgtgtacg gaatgcttag
    31921 ttgtttttcc agaaggaatt tccatcattc cgtggatggt tccgggaaca aatgagattg
    31981 gggaagcaac agctgaaaaa atgaaagaaa ctcgattggt tttatggcca caacatggaa
    32041 tctacgggtc aggaaaagac atggatgaag tctttggttt gatcgagaca gcagaaaagg
    32101 cagcagaagt ctatacgtac gtcaaagcgc aaggaccaat cttgcaaaca atcacggacg
    32161 aaaacctgtg gcatttagca gatgcgtttg gtgtcactcc aaaagcaggt tatttggagg
    32221 aagttcatac gaaagcaggt gtctgacatg gtgaaaaaag ctttttgcat gaaagtttac
    32281 ccagaaaaac aagaagaata caaaaaaaga catgatgaac tttggccaga aatgcgtaca
    32341 gcacttcgtg aacacggggt catcgactat tcgatctatc ttgatccgag tacaagtttg
    32401 ttatttggtt acttagtgat cgaagatgaa aagttatggg aaaaaatggc aaatacatct
    32461 atcaatcaaa aatggtggaa gtacatggaa gatatcatgg agacaaatcc agatgcctct
    32521 ccagtcagtt gggaattgat gccagtcttt gaactgtagc aacaagcaac tgggcaaccg
    32581 gttgcttggg gttctttcgt ttttattgtg aaaaataata caaattagga ggaatagaaa
    32641 atgagtaatc gaatgatttt aaatgaaaca tcgtattttg gaaaaggagc gattgagtcg
    32701 atcgtccctg aatttcagca gcgaggattc ggcaaagcag ctgtcattac tgacaaagga
    32761 ttgatcgagc acggtatagc aacaaaagta acagaacttc tagataaagc agcaatcgct
    32821 tatgctttat acgacgggat cgtacctaat ccaacgatcc aaaatgtaaa agatggagtg
    32881 gcttttgtta aagaggcaga ggcagactgt ttgatcgcaa tcggtggagg ttcgccaatc
    32941 gatacggcaa aagccattgg gattattttg acgaatccag agttttcaga cgtcattagt
    33001 ttagaaggtg ttgctgatac gaaaaatcca tgtttaccta tattagctat accaacgact
    33061 tctggaacag cagcagaagt aacgatcaac tacgtcatca cagatgaagt aaatcaccga
    33121 aaatttgttt gtgttgatcc tcatgatatc ccaatcgtcg cgttcatcga cagcgatatg
    33181 atgatgggaa tgccgaaaaa attggctgca tcaacaggta tggatgctat gacgcatgct
    33241 attgaagggt ttatcacaaa aggcgcttgg gaaatgactg atatgctgca tttaaaagca
    33301 atcgaaatca tcggccattc tttggaagct tctgtagatg gcgatcaaaa tgggcgagaa
    33361 aaaatggcat taggacagta tattgcggga atgggcttct caaatgtcgg tttaggactg
    33421 gtacacggga tggctcaccc gttaagtgca tggtacaaca ttcctcatgg cgtagcgtgt
    33481 gcagcacttt tgccaactgt catgaaatat aacaaagaat atactggaga aaagtaccgt
    33541 gagatcgctc ttgttctagg gattaaagga gcagctgaaa tgtctttaga agatgtaaga
    33601 gaagcggctt gtggtgagat cgatcggttg agcaaagcgg ttggaatccc agaaaccatt
    33661 tctgaattag gtgtaaaaga agcagatatt ccagcaattg cagaagatgc tttgcgagat
    33721 gtatgcacac caggaaatcc aagagaaacg acagtagaag aaatcattgc tttatatcaa
    33781 agtttgatgt aaaaacgaag cggatcttca acattaaatt gcctgtaaag agaaatggta
    33841 gtttatatct ggatatctct tttaaattga tggtatttcc gctataattt ttcttataaa
    33901 gaaataggag aatattatat ggaaaataaa aagccttatg gaactgtatt gatcaaagcc
    33961 tctaaaatac tagactattt agcggaaaac caagatgtct ctttgcagac gatcgcaaag
    34021 ggtgtgggaa tgacctcttc tacagtcttg aaaatactag atactttgtt gatgatcggc
    34081 tacgtaaata aaaattcaga gaaaaactat cgtttaggtg caaagcttat tcgctacgcg
    34141 aataaaaaca ttgaacaaat cgatttggta gagttgactt taccgttttt agaacagctg
    34201 cagcaaaaga tcgatgagac tatccactta ggggtattgg acaacaatga gatcttgtac
    34261 gtgaacaaac tagatccaaa acatcaaacg atccgcatgt cttcaaaaat cggaatcacc
    34321 cgaccattat acaattctgc gatgggcaaa gccgtattag cagaattcag tgaagagcag
    34381 tacactgatt atcttgaaaa gcatccgttg atcccttaca cagaatatac gatcaccaat
    34441 gcattacggt tgaaaaagga aatcaaaacg gtaaaagaaa cccacatcgc atttgacgat
    34501 gaagaaatcg aacgtgatat tttttgtata ggagcctcaa tcgtgaaaaa cgaacaaatc
    34561 attggtgcat ttagtgtaag tatgccgaaa tatcgtttaa cagatgagac aaaagaacaa
    34621 ataatcagtg ccctcaagca aacaaaaagc gaaatcgagc aaagaattca aaaataatga
    34681 aaaaagcgtt tgcaattttt cttttctgcc gtataatatg ggtatagatt tccaagttat
    34741 aaaataaaat ttctaaatag gaaataggtg gagaagatga aaaaaattga tattttatct
    34801 cgattgaaaa acgccggagt gattgcggtg gtacgcggaa aaagcaaaga agaagctttg
    34861 aatgcttgcc acgcaatcat caaaggtggt ctaactggta ttgagttgac ttttacagtc
    34921 cctcaagcag atcaagtaat caaagaactt ttgtcattct acaaagacca accagaaata
    34981 gtgattggcg caggcacagt attagacgct gtcacagctc gcttggctat cttagctggc
    35041 gcagagtata ttgttagccc ttcctttgat caagaaacgg cagagatgtg caatctgtat
    35101 caaattcctt acttaccagg atgtatgact attaccgaga tcaaaacagc gctgaaaagc
    35161 ggggtagata tcgtcaaact atttcctgga agcgcttatg gaccaagtat catttctgcc
    35221 ttcaaggcgc cgatgcctca agtcaatatc atgccaactg gcggtgtgag tctggacaat
    35281 atgaaagagt ggttcgatgc tggtgtcgta acagtcggag taggtgggaa ccttttagca
    35341 cccgcagcta caggagattt tgacaaagta acggaagtcg cgcaacagta tgcagcaaaa
    35401 atgaaagaaa tcaagaggta gctgcttatg ggaaaagtag tgacactcgg agaaatcatg
    35461 ttgcgattgt ccacagagca gggaacccgt atcgattcaa gtgaacgatt cgctgcccat
    35521 tatggcggag gggaagcaaa cgtagcaatt tctttagcta attacggaca tgaagcgatt
    35581 tttgctacaa aagtacctga taacgcattg ggaaaagcag tcaaaaagca cttgcaatgt
    35641 tatggagtag atacgaaaca ggtattatct ggtggatcaa gactaggtac ttattatatg
    35701 gaaactggta gtggcgaacg cgccgcctca gtgatctatg atcgtgccgg atcaagtttt
    35761 gctgaaatgg atcatttgga atggtcgcta gaggagttat tccaaggaat agatatattt
    35821 catgtttcag gaatcacccc agctttgtct aaaaaatggc aaaaaatgac gaaacaggtg
    35881 atagaagctg caaaaaaagc tggctgcctg atcagcttcg atatcaacta tcgtggaaaa
    35941 ttatggacac agcaagaagc cagcgaagta atccatcagc ttttaccttt agtagatatc
    36001 tgctcagctg gaaaaatgga tgctctttat ttgttaggta tcgagaaagc gccagaagca
    36061 gaagaacaac ctctaattta ttattatcaa aaaatgcagg aaaagttccc gaatattcag
    36121 gtgttttatt cgaccaagcg taccgttcat tcagcaagtg aaaatgaatt gaaggggaca
    36181 ttgtggatga accatgaata tgtggaatcc caagtccata gaatcacccc aatcgtggat
    36241 cgagtaggtg gcggagatgc ttttgcaggt ggcgtgctcc atgggatttt atccaatatg
    36301 tctccgcaag aaatcattga ttttgctacg gcagcttctg ccttgaagca tacgatccat
    36361 ggggactgca accaatttag tcaagaagaa gtcgcacaat ttttagcatg cggctctgga
    36421 aaaatcattc gatagaagga gtagaagaaa atgcaagaaa tggaaacacg ttatacacac
    36481 agtccagaag atatccgtca ttattcaacg gaacaattga gaaaagaatt tttagtagaa
    36541 aaggtatttg aaccagggaa aatcagtttg acttatacgc acaacgatcg aatgatcttt
    36601 ggtggagtga cacctactga aaaagaatta gaaatcacat tagacaaaga actaggtgtt
    36661 gattacttct tagagcgtcg tgaactaggt gtgatcaata tcggtggacc aggattcatc
    36721 gagatcgatg ggcaaaaaga agaaatgaaa aaacaagacg gatactatat cggaaaagaa
    36781 acacgacatg ttgtattttc ttctcaagat gctgaaaacc cagctaaatt ctatattagt
    36841 tctgttccag ctcatcataa atatccaaat gtaaaaatca gtatcgacca agtaaaacca
    36901 atggagactg gtgaaggcct gacactgaat gaacgtaaaa tctaccaata catccatcca
    36961 aatgtatgcg aaagctgtca attacaaatg ggttatacga ttttagaacc aggaagcgct
    37021 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctactttgat
    37081 tatgccaatg aagatacacg tgtgttccac atgatgggca aaccagatga aacaaaacat
    37141 ttagttgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
    37201 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
    37261 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
    37321 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
    37381 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
    37441 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
    37501 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
    37561 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
    37621 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
    37681 cccattcatc gtttcaaaag cagttattcc ggatatgatc gaaaaaggcg gcggtaaaat
    37741 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
    37801 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
    37861 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
    37921 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
    37981 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
    38041 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
    38101 cggaaaacaa ccttaaaaga gctttagtac actgtttagt ggagaggttg tgaaatcagc
    38161 ggattgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
    38221 ggacagaagc atttaactcc aagaaataag aaggaattca cgaaaattgc ttttcaaatt
    38281 tttgtgaatt tcagcttatt tccgaaggag ttgcttttac ttccgccgtt tgtacgtgtt
    38341 tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctcttttctg tgcgtcttcc
    38401 atacttcact aagtcgatcc ctcttgcttc gcaccaatcc ttcactggtc cggaaaagat
    38461 cagagattgt tggtgtaggg ccgcggtatt cgttgcgccg accatagtat aaagcagacg
    38521 aagctcttct tgccattgct tcatcagtat aatagtttct tctacgccgt gattcatcag
    38581 atgcgtcagt acggttcctg aagtacccac ggcatttgcg ccgagacaaa gggctttaaa
    38641 tatgtcatag gcattgcgga tacctccgga ggctaggatt tccatagaag tgtctgcttc
    38701 atttgcttca agcaatgaag agactgtcga ttgcccccag tcagctaaat aactcagttc
    38761 ccgcttggag cgacgggcgt tttcgatctg ggtgaagctt gttccgcttc gaccgctgat
    38821 atcaactgta tgaactccta aagcagctag atcattgagc gtttctcttg tcatgccaaa
    38881 accgacttct tttacgatga gggggacgtc aatggctgtt tgagtttctt gaataagtac
    38941 tttccaattg gtaaaatcac ggtctccctc aggcataacc aactcttgag gtgcattcaa
    39001 atgaatctgt aaggcatctg cgtgaaacaa tcggatagct tctttggctc tttcgacaga
    39061 agtccctgca ccgatgttgg caatgatttt cccgtgagga tattcttgtc gcataatcgt
    39121 ataagtatcg gctaaagaag ggactttcaa ggcagcgctg actgaaccag tggcaatcat
    39181 caaatctgct tctcttgcga cgattgcaag atcttggttg attttttttg ttttttcgct
    39241 tccgccagtc atcgcattga tataaaatgg gctgcttagt gtaaacccaa ctgcctgtgt
    39301 acttagatcg acatcagaaa ctgctatttg cggcaaaggg ttatggatga tccgtacgaa
    39361 gtcaaattcg tttttttgtt tatcatgaaa ggctttagct agtgaaacat gttcgtcttt
    39421 tcgattcatc ggttttcctt tctttgatcg ctatagacat gtaacggcag tggagtgatt
    39481 tctgcttttt cccatgcact cattaaagga agaatgccag atttctggtc aacaattacg
    39541 attccgcaat cgcccccacc tgcaccagaa gattttgcgg ctccttcata ctgttcagct
    39601 aaattacaca atttgttcaa agcaggcgtt tcgatgacga ccccagtgat tgcagataaa
    39661 tcatgcagta attgtcggtt ttttcgaatc atctgttgga tcaacgttac attattttct
    39721 ttaaaccctt tgatcatttc gttgacacat tctgtactat tttttaagaa aagctgataa
    39781 gccaccattt tatcttctct cgaacggtga acttgatcga ccaaatcaga agtagaggca
    39841 gggctacccg tccaaccaat caataaacgt aaatcttcag gagcaatcaa tggctcaatg
    39901 gatagacctg gccaatccag ggcaagtaac tcactgatag aatgctgagt ttcttgttct
    39961 tggagccaag gatggtcgaa ggttgagaaa gcgatccagc caccataaca gctagcagcg
    40021 atgtcgccgc aggaaccatt atcttgaact gctaaattgg ctagtgctgc aatcttgaaa
    40081 atctccaact gagataaatt caacgcgtaa aaaacattca aggctttgac cgttgcaacg
    40141 gtcacggcac cgctagaacc caatccatat ttccggccat ttgaactgtc taactcactt
    40201 gttactttca gatcataaaa ggataaaaga atgttttttt cttgtgcata cttttcagtc
    40261 aagcgaatag cagcaaggat ataatgaaaa ggattttccc gaatatctaa aactaattct
    40321 ccgttgcgtc ttgtccaacg tacaggcatc ccactatatt gtgcagattg gatacttccg
    40381 acttttcgtg cagattctac agtgactgtc acgaattgat cgactgcagc gataactgct
    40441 ggatggcctg tttcaacaac tgcatattct ccggcaatat agagtttgcc tggtgcagat
    40501 acttcaatca taaaaatctc cctttattca ataatcgcaa ttcctggacc agcatgtgca
    40561 gtaattactt gttctttgct aaataatttg gtaaatgttt cttgtacctc agataaatgg
    40621 ctgttttcca ctaaaacttt gacattaggg ccagcatcca tcgtaaagta gcatggaatg
    40681 ccttgctttc tcaattgtcg caccgcatcc atcgctttta agctgtcagg agaccagtaa
    40741 gtgaatggag gctgggcagc taaagtcgtt ccatgcattt ttagtccgtt tctttccatc
    40801 gtttctccga gaagttggaa atcttttgtt ttgattgctt gtttcaattg atatagatcc
    40861 ccttcaacgg aatctaacca gccttgataa aaactggatg tttcgactgt ccgacgcatc
    40921 ccatttctgc tggacacttc ttttttctgg tcgttgatca aaacgaaaac cattgctaaa
    40981 tcgtcttcga aagaatcgga agggactggc ttagcgtaag aacttaagtc gtcatggcct
    41041 ttttcccatt cgacgaaacc accgaaaata cttcggcaag ctgaaccaga cccgcgtcga
    41101 gcaaatcttg aaagagagag atcatctaat ccaagcttaa gagcagtatt gcaagcgcct
    41161 gctaaagcag ctagcccgct ggcagaggag gctaatccag ctgcggtagg aacgaaattc
    41221 tgactgatta ctgaagcttt tagtgagagg ccggcttctt tacgaaaaag gtcaagaaac
    41281 tgactgactt tttttgttgc tttttcgtct tgcaaggtgc catctagata aaattcatct
    41341 accatatagc tatcagaaaa gatgacttct gtctctgtat aaaatgcatc caatgttaac
    41401 gaaaggctgt tgttcattgg aaggatgagt tcttcatttt tcttacccca atatttgatt
    41461 agagcaatgt tggtatatgc gcgtgctttg cctttaaaca taggtatgta ctcctaatcc
    41521 ttgtatccaa gtttcagcag cacccgcatc ttcaagtgct tgactgattt cttgggcttt
    41581 tgtttttgtt tgtgctaatg caatcataca gccaccgcgt ccaccgccag ttaatttggc
    41641 acccaatgcc ccggtatctt ttgctgtttg gatcaataga ttaagaaaat cattgctgat
    41701 tgtcagcttt tccagaagtg attgtgattc atccattgtc tgtgccaacg tttctggtga
    41761 attttcaatg attgcttgtt ttgcttgctt cgtcaagtat cctaattttt ggatcatttg
    41821 tccagtttca tgaggctggg tttcaaagag gtgagcgaca tctttgacgg cttctcttgt
    41881 ttgtcctttg attcctgtat cagcgacaat caaaaaagca tcgatgttca aagaaaagta
    41941 gtcgaaagga tgcccttttg taaaataaat cggctgcaag ctgctagtag cggctgcatc
    42001 gattccacta ggattaccat gggcgatttt ttccgaaagc tggacatttt ctaatagtat
    42061 ttcacgagac aaaggaaatg ctaagtaatc ataaaaagca cgagtgactg ctgtggcgac
    42121 tgcagcgctt gatcccattc cacgttcagc aggaatcgtg ctttcaatgg tcagttgcaa
    42181 gtttttttgg atgtcatgct gtttttttaa gtgaatgaat aattttttga tattttttaa
    42241 tgcatgagga gcttcggcta aatttcctga atagtaggaa gaaaccagtt gatcgtccat
    42301 tgcatccagc tcttcaagga atgcggtgac ttttgttgca tagaaaggaa aggcaatcgc
    42361 tggttctcca taaacaaccg cgtgctcgcc catcaatatg atctttccgc ttgattctcc
    42421 ttggccatag tttgccataa tctaactcct tctcacgtaa aagtgatttt taagcaaaaa
    42481 ataaagcgcc caaaacgagt tgcgctgctt cacgaatttt tttgtccact aagggtctca
    42541 aaaaacattc ctcctttatt ttactaaaac catctcagaa gtaaaactaa gaatacgttt
    42601 tctaaagaaa aaacactttt ttcttaagaa ttttttgact ttttttcttc tttgactaga
    42661 aaaaaataca attgacacta ctagatattg ggggtatatt taaaaaggtt ggagttaaac
    42721 acaatattta gtgatgaagt gagctggagg taaatgagcg tgatcgtttt agcaggtaca
    42781 attggtgccg gaaaatcaag tttaacagaa atgatggctg aacactttga tagtcaggca
    42841 ttttatgaat cgattgatga taatgaagtc ttgcccttat tttatgcaaa ccctgaacag
    42901 tatgcttttt tgctgcaaat ttattttttg aataaacgtt ttgcaagcat caaacaagca
    42961 atgaaagatg acaataatgt attggaccgt tcgatttatg aagattcact gcttttccat
    43021 ctaaatgcag atctaggaag agcaacagaa acagaagttc gtgtgtatga tgaactgctg
    43081 gaaaacatga tggaagaact tccctatgct gcccataaaa aacatccgga tttattagtt
    43141 catattcgtg tatcttttga tacgatgttg gagcgtatcg aaaaacgtgg tcgttcgtat
    43201 gaacaattgt cctttgatcc aagtctatac gattattaca aagaattgaa cagacgttac
    43261 gatcaatggt atgaagaata caaagaaagt ccaaaaatcc aaatcgatgg agaccgctat
    43321 aattttgtgg aagatcctca agcaaaagaa gaagtcttga aaatgatcga agaaaaatta
    43381 gcagagattc gccaaaccaa agtagccatt tgatttacag ttgctcaaat aaagggtaaa
    43441 cctttatttg agcgactttt ttctttatac tggaaacaaa tgtcaggagg atagagtatg
    43501 cgggaaaaac actattttaa tcaagaccgt aatttttgga tcaatgtaca gcaaaacgat
    43561 gtgctagctg ttaaaaactt aaaagaagaa tatggcatta gtgacgagat gctgacttat
    43621 tctctagata aaaatgaacg ggcgcgcgta gagtacgatt cttttgatga agcgttactg
    43681 cttgtatcaa atgttccgca ccagcaaaaa gtcgataatc attatgaaac tagtcctatc
    43741 gcttttattt taaaagaaga cggtttgttt acatttacca cgccgaatac cgagtacgtg
    43801 attcgactaa ttcgatcgct tttggaacga atgccagaca tgagtgtata cagtttattg
    43861 tttcgtacat tgtttttgat ttctgattcc ttttttcctt taatcgagga agtgaatagt
    43921 gaacgtcagc ggcttaatct gaaattaaga gaaaaaacaa cgaacaagaa tctgctgcaa
    43981 ctttcagatt tagagatcgg tttggtttac ttagttacag gaacaaaaca aaatgtagta
    44041 cttttagaac aaatcaaggc attggctatt tatcgcaagt tgtcagaaaa ggaaaaagaa
    44101 gagttggacg atgcgctgat cgaagcacga caagcagttg aaatgacgaa cttagcttcg
    44161 cagattttag accagctctc tggcacttat aataatttgc tgaataacaa cttgaatgac
    44221 acgatgaaat ttttgacggt ctggtcgctg atcttgaccg ttccaactat tgtgacagga
    44281 ttcttcggaa tgaatctcca gttacccttt acccattccg ttttcggatg gggaatcgcc
    44341 ttgatcatca gtttggtctt atctatttgg atgcttattg cactgtggcg gcggattcgt
    44401 tgatctagtc tttgatctgc aaaccaaggg cttgggcgat cacaagtgct tgctcttggt
    44461 tgacgattag tcccgccaaa gaatcttgag ataaagcgat tgctgtgaag aaattagtcg
    44521 taaagtctaa tcccttcaga gaagttcgaa accagttcga accggtcaac tgattgtcag
    44581 acaaaaaaag gtttttccaa gtgacctcat aaaattcgct gtcttctaat tgacaatgat
    44641 cgaaggagac agctttcaag tttgtactac taaaggaagc catcgaagca atacaatcag
    44701 tgaaagtaca gtctcgaaga taactttcgg caaaattcgt acctgttaat ttacattgat
    44761 gaaacacgac ttggtggaaa ctgccaccaa tccactccag attggaaaaa tcacattttt
    44821 caaaaatgac attactgcat tcaaaatgac gtaaaactgt tttttgcatc gtaagttttt
    44881 ggatatgact ctctcttaat accatatttt tggcatcgag atagctttgg tcacccccgt
    44941 tgatcagaag accagagaag atctcctcat cttctaaata aaactctccc tttgtaagaa
    45001 gaggaagaga aggtggaata ggttcgattt ttttcatgtg ggttcacctc gtttttcttt
    45061 agtatagcaa agaagcggga atcactgcta gaaagtctgc taatctaatc attttcagaa
    45121 aaggaagaca aactgtatga aaaacattga caaaaagatt agtttttgct atactatgat
    45181 ggttgagaaa taaaagcaga agcacccgct tctcgcctta gttgataatg tcactgggct
    45241 agatagtttt cactggttct attaggtgaa gtacgcgcgg gttctgtaag agaactcgtt
    45301 tttttattgc cgtttattcg gtaagctttt ctttctctca taatatttgg aggtgaatga
    45361 ccatagcaaa ggatatgatg gttaacgacg gcattcgtgc acgagagtta cgattgatcg
    45421 gttcagatgg tgagcaatta ggagttaaga caaaagcaga agcattgaat attgcagaac
    45481 aagcaaactt ggatcttgtg ttagttgccc ctggtgcgaa accaccagtt gcgcgaatca
    45541 tggactacgg taaataccgt ttcgaacaac aaaagaaaga ccgcgaagcg cgtaaaaaac
    45601 aaaaagtgat caatgtaaaa gaagttcgct taagtcctac gatcgacttg aatgacttca
    45661 atacaaaact tcgtaatgca cgtaagttct tggaaaaggg cgataaagtg aaagcttcta
    45721 tccgtttcaa aggccgtgcc attacccata aagaaattgg tcaaaaagtc ttgaatcgct
    45781 tagctgaaga aactgcggat attgcaacag tggaacaaaa agcgaaaatg gacggacgca
    45841 gcatgttctt gacgctggca ccgaaaaacg acagtaagta atttgaatga ctaactggct
    45901 gttggacagc cgatagtcgg ataacgaaag attttgagga ggaaatatag tcatgccaaa
    45961 acaaaaaaca caccgcggat cagcaaaacg tttcaaacgt actggtaaag gcggattgaa
    46021 acgcttccgc gcgtttacaa gtcaccgttt ccacggcaaa acaaaaaaac aacgccgtca
    46081 attgcgtaaa gcaagcatgg tttctagtgg cgacttcaaa cgtattcgcc aacaactagc
    46141 aaaaatgaaa taagaagtac aaataacaga atctgaagat ttatttttat taggaggaat
    46201 tatacatggc acgtgttaaa ggtggaacag taactcgtaa acgtcgtaaa agagtgctaa
    46261 aattagcaaa aggttactac ggttcaaaac acactttatt taaaacagca aaagaacaag
    46321 taatgaattc atacaactac gcatatcgcg atcgtcgtca aaagaaacgc gacttccgta
    46381 aattgtggat tgctcgtatc aacgcagcag ctcgtatgaa tggcttgagc tactcaaaat
    46441 taatgcacgg tttgaaagtt gctgaaatcg acatcaaccg taaaatgtta gctgacttgg
    46501 ctgttaacga tgcagcagca tttactgcat tggctgacca agcaaaagaa gctttggcta
    46561 aataagctaa acaattataa agaggaaaga agataacgaa aagttatgtt ctttcctctt
    46621 tttttattta caatcctcca tcaaatgaaa caggacaata acgtataagt ctaaaatagc
    46681 ataattaagg ttttttaatt gaaaactgaa catttatggt caccctttaa tgaaagcgct
    46741 aactacataa gttagaatga gtttgactga tatattagtt tgcttatcag aaaaacaaaa
    46801 tggacggagg aataacatga gtgatattat tattggtatt ttgttagtta tctcattctt
    46861 tttcatggtc tggtactgtg taaaaggcta caacttgatg gtcggctttg ccatcatggc
    46921 tactgtatgg atgggattag cgctagtcgg aaacacgttt tcaccaaacc cggcgatgga
    46981 agggcaagga gtaattgatg tgttgacgca tatctatacg acggggccag ctgagtacgc
    47041 caaatcgatc ttggtaaatg tcttctttgg tgcctttttc gggcgtgttc tagtagatag
    47101 cggaatcgca gctaccctga ttcgaaaggt agttgaactt ggaggggaca agccaagaat
    47161 cacgatgagc ttactatgta ttgtaaccgc tgttatattc atgtcgatga caggaatcgg
    47221 accagtgatt tctattgcag ttattgtgtt gccaatcttg atgtcattag gtatttcagt
    47281 accggttgca ctgttctcat ttatgggctc gattatggct ggaatctttg ctaatatcgt
    47341 caatttcaaa caatatcaaa cgatttatgc tggtttcaat ccagcggctg aaagctatac
    47401 ttataatgac tatttccaaa ttggtatgat tgggatgatt gtaagcttag ttgtggtact
    47461 aacggtcgct aatatatcaa tgaacaaaaa gaaacgctat gcaatggcag caaacgtacc
    47521 agctgaaggt ggcgacgcac caatgatctc atggttagcc gtattattac cagttttagg
    47581 ggtcgttctt ttagatcttc caatcatctt aggttttatt ctagcaggta tctgggcatt
    47641 gctgtttact ggaaaattgc gcggaggtta taaagaaatc tgtcgtcagt ttgcgaaatt
    47701 atttacagat ggcgcagtag atgttgcgcc aatggttggt ttcttgatga ccttagcgat
    47761 gttcaataat tcagctgcgt atgcttctcc ttatttttca gctatttttg gagattggat
    47821 accgcaatca ccattagtct tggcaatcgt atttgctata ttgacaccac taggattttt
    47881 ccgcggtcca atgaatctag tcggttcagg ctcagctatt ttagctgtcg tattagctgt
    47941 taatccaacg atgtcgccag cctttttgtt cccgttattt gcaatcacaa cgattgctcc
    48001 tcagcattta gatattacac aatcttgggt tgcttgggga ttaggttata caaaagtaac
    48061 ttctcgagaa tatatgaaga aatccatccc aactggttgg atcatcggag cgatcttatg
    48121 cctgatcacg ttcttattgt acgggaacgc ctagtaaaca aaacgtcgat aaaaacgatt
    48181 agatgaatgg aggaaaagaa aatgaaacgt tttgtgcgaa tgggaattga tgttggcggt
    48241 acacatacga aagcagtcgc aatcgataat gcgacgcacg aaatcatcgg aaaatcttca
    48301 gtgaaaacaa cccacgatga tgtccgtgga gtagccgcag gtgtcgttca gtcttttcaa
    48361 aattgtttgc tggaaaataa tatcagtcca gaagatgttg tgtttgttgc acattctacc
    48421 acacaagcga ctaatgcact gattgaagga gacgtagcaa aagtcggcgt aatcgggatg
    48481 gcaaaaggtg gattagaagg ttttttagca aaacgacaaa ctagattgaa cgatatcgat
    48541 ttagggaata agaaaaaaat agagattgtc aatgcattct tacctgtcaa acacttaaat
    48601 gtagaccgtg tttcagaaac gatttcttct ttagaaagag aaagagcgga ggtcttggtt
    48661 tcttctatgg catttggagt agataatggg gaaccagaac gagttgttta tgaagcagca
    48721 tcggtaaaat cgatccctac aacgatggct tctgacatca caaaattata tggattgaca
    48781 agaagaactc ggacagcagc aatcaatgca tctattttac caaaaatgct agatacggca
    48841 acctctacag aagattccgt acgagaagca ggggtcaatg tttctttgat gattatgcgc
    48901 ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac gacctgtttt aaccatgctt
    48961 tccggtccag cagcatcagt catgggctct ttgatgtact tgagagcgtc taatggtgtt
    49021 tattttgaag taggcggaac aacaaccaat atcggggtca ttaaaaatgg acgtccagca
    49081 atcgattatt caattgtagg tggccaccca acatatatct cttcattaga tgttcgggtt
    49141 ctaggtgtgg caggcggttc gatggtacgg gctaatcagt caggaatcat cgatgtcggt
    49201 ccacgttctg cacatattgc cggtctagat tatgcagtat ttacggagac agaaaaaatc
    49261 aaaggtccta aggttgaatt tttctctcct aaagaaggag accctgctga ttacgtcaag
    49321 gtagtgatgg aagacggaga agaagtgact attacaaata catgtgcagc caatgtcctt
    49381 ggattagtgc aagaagaaca tttttcttat ggaaatgtcc catctgcacg aaaagccatc
    49441 caagcattag ctgattattg tcatacgacg gttgaagaca tcgcagaaca gatcatggaa
    49501 aaatcctatg ctaagattga accggtgatt ttagaattgg cagacaagta tcacttggag
    49561 aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg cctcattgat tacttacttt
    49621 agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg ccgaagtgat ttcttctatt
    49681 ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta tcatcccttc gccaagtaaa
    49741 gaagatatac gatcgttgaa aaatgaagcg atgaataaag cgattgaatc aggcgctaca
    49801 ccagaatcga tcgaagtcca cgtagaaatc gatccacaga cttcaaaagt aacggctatc
    49861 gctactggtt ctacggaagt gaaagcaacc gatctaacca aggagatcac aacagaagaa
    49921 gcactagagc ttgctgcaga ggatatgcga ttaaataaaa atgaagtttg cttattggaa
    49981 aatacacctt tcttttatgt atgcggcgaa cagaatcgtt caaaaaatgc tggaagtctt
    50041 cggatcatcg atcaaaaagg atttatcaag gtccaacgtg gacatgcttc atgtatgaaa
    50101 acaactgcag ccaattatat gacagctgtc gaacaacttt gggaagatat ggcagtttat
    50161 caaactgaat tgattgcccg accagaattt tatctctgct taggagcaag agtctctgat
    50221 tttacagcga cggatctgga acaactacag cttttgatgg atctggaagt atctactatg
    50281 gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca aacaaacata agttagaaga
    50341 gttactaaac gaattacaac agatccccga agaaatttgg ggattctacc agtttcggcg
    50401 agatttattt tggaaaaaga ttcctttgtc caagcagaaa attcttatac aacaatcaat
    50461 cgattgtggt atcgaaacgg catgtagcat caaaaaaaag tacccttttg ccgatgtagg
    50521 agaaatttgt gaacaaatgg ctgttccaat cgtttcctgt gaatcagagc aaataaacga
    50581 acgaatcact tttgcaactt atgcagaaga tgaagggatt cgattgatga cggagccgtt
    50641 agaaaaatta aaatgttcag gactagcttc gatctctaaa gaaacagcgc aagctttgat
    50701 tattggacat gaactatttc accacatcga agcttctgtt aagggaattt atacgcaaag
    50761 cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat caatcaaaca ttcgtgcatt
    50821 gagcgagata gcagcgatga gtttttcaaa agagatgaat caaagcgatt tttcgcctta
    50881 tgtattagag gcagtcttac tttggccata taatgaaatt cgtagccaag ggatcttaga
    50941 agaaataaaa aaaatagaaa aaagatgtac ggagtatgat ttagattaca aataaagaga
    51001 ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata aacgatgttc tcctgtcaac
    51061 tcctcggtca caagtcaaaa tattcaacaa acatgagaag ctgttttttg aaattgttcc
    51121 ttgtgactcg gagatgaacg gcaggctcac gacctcttgg aaaacggtgt tcaaaagctt
    51181 gcatctgcgg taataagtcc agccaagtta aaaacataag aagctatttt tacttggctg
    51241 ttcttattac ttgatgcagg gcagcttttt cacaacctct caataaacgg tgtgcaatca
    51301 gctgaccctc ggtattagct caaaaaaaac ggcaaaatat gaggaactat tttgccgttt
    51361 ctttctctaa tactcaggtc aaaacgctga ttccaaaacc tcttatttga tgggtctgtc
    51421 ctgaatatct tcaaaataat cggggtaatt atgaatcacg tattcttgct cctcgataat
    51481 catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca aagttatgat caatcattga
    51541 ttgaagcatt gtctgatgtt catctaatac tcgaccccag tctaattctg aaatcgtcaa
    51601 acgtaaccat ctgaaacgcg tactataact ttctagccaa tcccaaattt ctcgttttcc
    51661 agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag cttttttatt
    51721 ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag cttgatcttg
    51781 cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt cccgcgtata
    51841 acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg actgtggaat
    51901 ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg gtgtacgccc
    51961 gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg ctaactcgga
    52021 ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat tgataaccat
    52081 ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta caaatcgtgt
    52141 gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta gagcctcgaa
    52201 aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac gaccgccgat
    52261 tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat tgtctaagtt
    52321 tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt taacgacata
    52381 catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca atacaagaaa
    52441 aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac gcttggaata
    52501 actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa tcagaaagta
    52561 aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt cccacaattt
    52621 cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg atgctcactt
    52681 tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg gggatgatga
    52741 tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag gctacccgag
    52801 cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga tctgtttcat
    52861 aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc tcgttttcca
    52921 ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt tgtgtcggca
    52981 aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga taaatagctg
    53041 ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc atcccagcta
    53101 aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg gtgtagaagt
    53161 gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca ccgatttcct
    53221 gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa ctcaaccgtt
    53281 gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc aaaagatgat
    53341 tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct tttgattggt
    53401 tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat ccgggaccaa
    53461 tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc gtccaaacta
    53521 caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca tcagttacta
    53581 cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg attgctccgc
    53641 catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta tgtgaaattt
    53701 ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc taaaaacctc
    53761 cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa aggaattgtc
    53821 agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa gacttaaata
    53881 agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca cgtaaagaag
    53941 acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat gactcaaaag
    54001 taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat atgttagctt
    54061 atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg catattgatc
    54121 ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt cctgcaggag
    54181 atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc tttgcacgcg
    54241 gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag atcattaaag
    54301 aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat gaagaaagcg
    54361 gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt ggtttctcac
    54421 cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta gctttacgct
    54481 tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga cttcgcgaaa
    54541 atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat gcagctatcg
    54601 cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga acgattgaag
    54661 cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt gcaagcccac
    54721 aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat gaattcctag
    54781 gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat ttttatggag
    54841 aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg aatgctggac
    54901 tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg aatttccgtt
    54961 ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca gtggggcaag
    55021 aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta ccaatggatg
    55081 atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa aaaggctacg
    55141 aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt gcttatggag
    55201 cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg agtcttgatg
    55261 acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg aaataattaa
    55321 ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt ttttactttc
    55381 tactgtggta ttcatgataa acatgatata gattcaattc atattctttc ttagcgttcg
    55441 tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa gtcatcaatc
    55501 cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata tattcgcgaa
    55561 tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa atcccaaaga
    55621 aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact ttgaatccgt
    55681 cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa tcgattggga
    55741 tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt tcgatttgaa
    55801 aaccagaact cataatagga aaacttttga caaagatacg attgaatcct cgatagccgg
    55861 tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta acaaggttat
    55921 ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt gataggcgat
    55981 caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca tgtacagctt
    56041 ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg atatcaaaga
    56101 acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg attgctccac
    56161 cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca taaatatctg
    56221 cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct tcctcattgt
    56281 aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct ccttaactat
    56341 agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag tttttcccgc
    56401 taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc tctatctaaa
    56461 aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg gtgcgtattc
    56521 gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa atttctaata
    56581 cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca tcttctattc
    56641 gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata ggttcctttt
    56701 caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac ttgatatttt
    56761 caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt gaagctaccc
    56821 cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag cttagttcgc
    56881 tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg tgcttatttt
    56941 accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat aagctataat
    57001 aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat aagtctgaga
    57061 ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt caacgaatca
    57121 aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc gcttatatgg
    57181 aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc ggcatttttg
    57241 gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca catatggatg
    57301 aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc gtacctctag
    57361 gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc tctactggta
    57421 agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga acaagcggac
    57481 aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca agagaagagg
    57541 caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa acgatcaaaa
    57601 cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc tgtacaatgg
    57661 tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg attgcaggcg
    57721 cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat aaattcaagc
    57781 cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg aaaaatggca
    57841 cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt ttgatcatgc
    57901 ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc ccttatcaat
    57961 actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac gaaggaatcc
    58021 caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg atgttcagta
    58081 ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt ttggataaat
    58141 caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata aaatctggca
    58201 aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg ttagttcgaa
    58261 agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac aaagaatgat
    58321 tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga gtgtattttt
    58381 ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc ctgaaattga
    58441 aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca tagatgtagc
    58501 cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg gaaaagaatt
    58561 aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt tcctagaaag
    58621 tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat ataagaatat
    58681 tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag cggtagaagt
    58741 ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc aattttataa
    58801 ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg aaacagccaa
    58861 ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa atatagaagg
    58921 aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa aaaaatatga
    58981 gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct ttatgacagg
    59041 aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg tttctgacga
    59101 agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa tcaattaact
    59161 aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg aataaatggt
    59221 gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca gtgaatgcaa
    59281 ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga tttttgctta
    59341 taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata aaggaacaga
    59401 aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag gtcaagttgt
    59461 tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg gccaagtaag
    59521 cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag gattcgaaga
    59581 acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa catgcgtccc
    59641 acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg gtacagtatt
    59701 gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg ttgcaaaacc
    59761 aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag gggtagatag
    59821 ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag caaaaaaagg
    59881 aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat aaagaggttg
    59941 tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat tttgttttaa
    60001 tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc cttcatagta
    60061 agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa gttctacacc
    60121 agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca atccagcttt
    60181 ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta cagatgaagg
    60241 aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga ccattgtgat
    60301 acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga tattcactac
    60361 atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag tagattcaga
    60421 agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat taactaaagg
    60481 accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga tattgactgt
    60541 ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga ttcctgaagt
    60601 gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag gctcaccaac
    60661 cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg aattgatctt
    60721 caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta ccttcgtacc
    60781 gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat tcgtgactac
    60841 ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat aagcttccaa
    60901 attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc caaaaccgct
    60961 tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa ctttatctac
    61021 cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg tttcaccggc
    61081 agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca tcgctgcata
    61141 aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca gacctaactt
    61201 acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt ttctatccat
    61261 gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat ttgttgactt
    61321 tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc attgttttca
    61381 gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc ggataggatt
    61441 ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa attcttataa
    61501 aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt tttctgtgga
    61561 taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa atagccaaaa
    61621 taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag accaaaggag
    61681 agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat acctgatcca
    61741 aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat tgatcgaagt
    61801 tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag taaaagaaaa
    61861 agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg ccttagcaat
    61921 tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat taggtatcca
    61981 aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg aagccgtcat
    62041 gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc aattgatgcg
    62101 tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg ctccatacca
    62161 caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata gcaataggta
    62221 gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa gtaagttgca
    62281 aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata cgtaaaattt
    62341 tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt ttaaaaaatc
    62401 tattagagga tatggatgat attgaagaaa actatctaaa tggactggga aaacaaatat
    62461 atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt tagctttttt
    62521 gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt ttttgaaaag
    62581 gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg ctttacttaa
    62641 tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt tattggtatc
    62701 ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg agtctggagg
    62761 gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata aaaaaatttt
    62821 tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca taattactta
    62881 caagaatatc gttga
//
LOCUS       DASJTC010000004        62124 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.004, whole genome shotgun sequence.
ACCESSION   DASJTC010000004 DASJTC010000000
VERSION     DASJTC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.004"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            47..2281
                     /gene="pcrA"
                     /locus_tag="U4B19_000223"
     CDS             47..2281
                     /gene="pcrA"
                     /locus_tag="U4B19_000223"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEN2075993.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            2298..4334
                     /gene="ligA"
                     /locus_tag="U4B19_000224"
     CDS             2298..4334
                     /gene="ligA"
                     /locus_tag="U4B19_000224"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEN2075994.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            4440..4745
                     /gene="gatC"
                     /locus_tag="U4B19_000225"
     CDS             4440..4745
                     /gene="gatC"
                     /locus_tag="U4B19_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEN2075995.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            4745..6217
                     /gene="gatA"
                     /locus_tag="U4B19_000226"
     CDS             4745..6217
                     /gene="gatA"
                     /locus_tag="U4B19_000226"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEN2075996.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            6214..7644
                     /gene="gatB"
                     /locus_tag="U4B19_000227"
     CDS             6214..7644
                     /gene="gatB"
                     /locus_tag="U4B19_000227"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEN2075997.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            7654..8709
                     /locus_tag="U4B19_000228"
     CDS             7654..8709
                     /locus_tag="U4B19_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN2075998.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            8820..10196
                     /gene="rlmD"
                     /locus_tag="U4B19_000229"
     CDS             8820..10196
                     /gene="rlmD"
                     /locus_tag="U4B19_000229"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN2075999.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            10405..11241
                     /locus_tag="U4B19_000230"
     CDS             10405..11241
                     /locus_tag="U4B19_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2076000.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            11390..11611
                     /locus_tag="U4B19_000231"
     CDS             11390..11611
                     /locus_tag="U4B19_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2076001.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            11612..14329
                     /locus_tag="U4B19_000232"
     CDS             11612..14329
                     /locus_tag="U4B19_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEN2076002.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETWYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            14322..16277
                     /locus_tag="U4B19_000233"
     CDS             14322..16277
                     /locus_tag="U4B19_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2076003.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            16267..17403
                     /locus_tag="U4B19_000234"
     CDS             16267..17403
                     /locus_tag="U4B19_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2076004.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(17454..17657)
                     /locus_tag="U4B19_000235"
     CDS             complement(17454..17657)
                     /locus_tag="U4B19_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2076005.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(17673..19505)
                     /locus_tag="U4B19_000236"
     CDS             complement(17673..19505)
                     /locus_tag="U4B19_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2076006.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            complement(19508..19954)
                     /locus_tag="U4B19_000237"
     CDS             complement(19508..19954)
                     /locus_tag="U4B19_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076007.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            complement(19947..20897)
                     /locus_tag="U4B19_000238"
     CDS             complement(19947..20897)
                     /locus_tag="U4B19_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076008.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            21292..21909
                     /locus_tag="U4B19_000239"
     CDS             21292..21909
                     /locus_tag="U4B19_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076009.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            22006..22212
                     /locus_tag="U4B19_000240"
     CDS             22006..22212
                     /locus_tag="U4B19_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076010.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            22217..22531
                     /locus_tag="U4B19_000241"
     CDS             22217..22531
                     /locus_tag="U4B19_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HEN2076011.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            22532..23674
                     /locus_tag="U4B19_000242"
     CDS             22532..23674
                     /locus_tag="U4B19_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HEN2076012.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            23676..24218
                     /locus_tag="U4B19_000243"
     CDS             23676..24218
                     /locus_tag="U4B19_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HEN2076013.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            24279..26216
                     /locus_tag="U4B19_000244"
     CDS             24279..26216
                     /locus_tag="U4B19_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEN2076014.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            26427..27197
                     /locus_tag="U4B19_000245"
     CDS             26427..27197
                     /locus_tag="U4B19_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HEN2076015.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            27190..27534
                     /locus_tag="U4B19_000246"
     CDS             27190..27534
                     /locus_tag="U4B19_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HEN2076016.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            27527..29770
                     /locus_tag="U4B19_000247"
     CDS             27527..29770
                     /locus_tag="U4B19_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN2076017.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            29908..30117
                     /locus_tag="U4B19_000248"
     CDS             29908..30117
                     /locus_tag="U4B19_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076018.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            30114..30398
                     /locus_tag="U4B19_000249"
     CDS             30114..30398
                     /locus_tag="U4B19_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEN2076019.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            30398..31750
                     /locus_tag="U4B19_000250"
     CDS             30398..31750
                     /locus_tag="U4B19_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2076020.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            31747..32175
                     /locus_tag="U4B19_000251"
     CDS             31747..32175
                     /locus_tag="U4B19_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEN2076021.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            32349..32708
                     /locus_tag="U4B19_000252"
     CDS             32349..32708
                     /locus_tag="U4B19_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2076022.1"
                     /translation="MKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAINKR
                     YGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCKSC
                     HSKIHMTRRNTKDKTIM"
     gene            32842..33414
                     /locus_tag="U4B19_000253"
     CDS             32842..33414
                     /locus_tag="U4B19_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEN2076023.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            33429..34211
                     /locus_tag="U4B19_000254"
     CDS             33429..34211
                     /locus_tag="U4B19_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEN2076024.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            34211..35446
                     /locus_tag="U4B19_000255"
     CDS             34211..35446
                     /locus_tag="U4B19_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019116086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEN2076025.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            35520..35897
                     /locus_tag="U4B19_000256"
     CDS             35520..35897
                     /locus_tag="U4B19_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HEN2076026.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            35899..36093
                     /locus_tag="U4B19_000257"
     CDS             35899..36093
                     /locus_tag="U4B19_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076027.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            36077..36253
                     /locus_tag="U4B19_000258"
     CDS             36077..36253
                     /locus_tag="U4B19_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HEN2076028.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            36250..36393
                     /locus_tag="U4B19_000259"
     CDS             36250..36393
                     /locus_tag="U4B19_000259"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076029.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            36476..38041
                     /locus_tag="U4B19_000260"
     CDS             36476..38041
                     /locus_tag="U4B19_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2076030.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            38097..38831
                     /locus_tag="U4B19_000261"
     CDS             38097..38831
                     /locus_tag="U4B19_000261"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076031.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            38897..40225
                     /locus_tag="U4B19_000262"
     CDS             38897..40225
                     /locus_tag="U4B19_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008817775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2076032.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            40125..40880
                     /locus_tag="U4B19_000263"
     CDS             40125..40880
                     /locus_tag="U4B19_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEN2076033.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            40884..42095
                     /locus_tag="U4B19_000264"
     CDS             40884..42095
                     /locus_tag="U4B19_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2076034.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            42106..42417
                     /locus_tag="U4B19_000265"
     CDS             42106..42417
                     /locus_tag="U4B19_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN2076035.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            42414..42752
                     /locus_tag="U4B19_000266"
     CDS             42414..42752
                     /locus_tag="U4B19_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2076036.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            42745..43176
                     /locus_tag="U4B19_000267"
     CDS             42745..43176
                     /locus_tag="U4B19_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN2076037.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            43173..43514
                     /locus_tag="U4B19_000268"
     CDS             43173..43514
                     /locus_tag="U4B19_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076038.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            43517..44107
                     /locus_tag="U4B19_000269"
     CDS             43517..44107
                     /locus_tag="U4B19_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2076039.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            44117..44497
                     /locus_tag="U4B19_000270"
     CDS             44117..44497
                     /locus_tag="U4B19_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076040.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            44704..47265
                     /locus_tag="U4B19_000271"
     CDS             44704..47265
                     /locus_tag="U4B19_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2076041.1"
                     /translation="MADNFGLKIGVEGEKEFKNSLREINQSFKVLGSEMKLVSSEFDK
                     NDKSISSLSARNQVLNKSIDAQKEKITTLENALKNASDSFGENDKRTQNWTVQLNNAK
                     AQLNNMEREVANNEKAIDAMGTEEVDAAKKTDELGNELKDTGDEAEKSGSKFEKFGGV
                     LKGIGVAMGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTVQKNADNA
                     FKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKMGTDIESI
                     QNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFADVTEAIHV
                     IQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNVVEQFQNV
                     IKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGLLPTLIPV
                     VVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIITIVNGLIE
                     NLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQTGIQLLT
                     SLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALPQIITTIA
                     NAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQIITGLANAF
                     GEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIHSPSTLFR
                     DEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDSTFSMLDL
                     GAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRRRVAY"
     gene            47336..47737
                     /locus_tag="U4B19_000272"
     CDS             47336..47737
                     /locus_tag="U4B19_000272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076042.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            47814..48506
                     /locus_tag="U4B19_000273"
     CDS             47814..48506
                     /locus_tag="U4B19_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2076043.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            48503..52393
                     /locus_tag="U4B19_000274"
     CDS             48503..52393
                     /locus_tag="U4B19_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2076044.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            52403..52570
                     /locus_tag="U4B19_000275"
     CDS             52403..52570
                     /locus_tag="U4B19_000275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076045.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            52557..52964
                     /locus_tag="U4B19_000276"
     CDS             52557..52964
                     /locus_tag="U4B19_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HEN2076046.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            52965..53384
                     /locus_tag="U4B19_000277"
     CDS             52965..53384
                     /locus_tag="U4B19_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN2076047.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            53374..54282
                     /locus_tag="U4B19_000278"
     CDS             53374..54282
                     /locus_tag="U4B19_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2076048.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            54398..55435
                     /locus_tag="U4B19_000279"
     CDS             54398..55435
                     /locus_tag="U4B19_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEN2076049.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(55496..55786)
                     /locus_tag="U4B19_000280"
     CDS             complement(55496..55786)
                     /locus_tag="U4B19_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076050.1"
                     /translation="MEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIEAEEAL
                     YQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            56096..56269
                     /locus_tag="U4B19_000281"
     CDS             56096..56269
                     /locus_tag="U4B19_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076051.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            56330..57883
                     /locus_tag="U4B19_000282"
     CDS             56330..57883
                     /locus_tag="U4B19_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2076052.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            57847..58296
                     /locus_tag="U4B19_000283"
     CDS             57847..58296
                     /locus_tag="U4B19_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076053.1"
                     /translation="MRTTFEREVTRMNNTIPYGYVIKNGEINVDEQQAQQIREFFNIY
                     LEGVGLQEAGKRSGINKPHPFMKRILMNEVYLGNEIYPKIIDEDIFFQADLEQERRIK
                     FFKKRKEIKVPDTKVIPTHFKSGKITEKYSDPFKQAQYAYSKIKEVK"
     gene            58298..59848
                     /locus_tag="U4B19_000284"
     CDS             58298..59848
                     /locus_tag="U4B19_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2076054.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            59971..>62124
                     /locus_tag="U4B19_000285"
     CDS             59971..>62124
                     /locus_tag="U4B19_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076055.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDSL"
BASE COUNT        19935 a        11147 c        14101 g        16941 t
ORIGIN      
        1 gaaaatgcta taattaacaa gattgataaa agaaaggatg ttgttaatgt catccaaagc
       61 tgaattatta aacgggatga acccccgtca gaaagaagct gttcttcaca ctgacggacc
      121 attactgcta atggcaggtg caggaagtgg aaagacgaga gtgctgacac accgcattgc
      181 ctatttgatt gaggaaaaag aagtcaatcc ttggaacatc ctagcgatta cttttaccaa
      241 taaggctgca aaagaaatga aagaacgtgt caatgcgatt ctcgcatctg gaggcgaaga
      301 tgtctgggta tcgacttttc actcgatgtg tgtgcgtatt ttaagaagag atgttgattt
      361 tatcgggtat aaccggaact ttacaatcat cgattcttct gaacagctga ccttgatgaa
      421 acggatacta aaagaactga acattgatcc gaaaaaatat gatcctcgaa gtatattagg
      481 aacaatctct caagcaaaaa acagcctgca gacccctcag gattttgcaa aaatgcaagg
      541 cagttattac gaagagattg cggcaaaatg ttatgctgct taccaaaaag agcttcaata
      601 taatcaatgt atggactttg acgacctgat catgaatacg attcgattat ttgaagaaca
      661 tcctgattca ctcacatatt atcaaaacaa attccattat attcatgtag atgagtacca
      721 agatacaaat cacgcacagt atacgctagt caatttattg gcaggtagat ttaggaacct
      781 gtgcgtcgtg ggagatgcag atcaaagtat ttacggctgg cgcggtgcag atatgcagaa
      841 catcttggat tttgaaaaag attatccaga tgctgcagta attttgctag aacaaaacta
      901 tcgctccacg aaaaacatcc ttagcgcagc aaaccaagtc attgaaaaca atagcaatcg
      961 aaaaccaaaa aacttgtgga cagaaaacaa agaaggaaac aagatcactt actaccgtgc
     1021 agataatgaa cgagacgaaa cccgttttat cgtagatcgg atgcaagaag agattcgaag
     1081 caatcatcgg aattacggcg actttgccat cttgtaccgg acgaacgcac aatctcgtgt
     1141 aatggaagaa actctgttga aagcaaatat tccttataaa atggttggag ggcacaagtt
     1201 ctacgatcgt aaagaaatca aagatatttt agcgtatttg aatgtgctgg caaatcctca
     1261 agattcaatc agttttgagc gtatcgttaa ttcgcccaaa cgaggcatcg gaccaggatc
     1321 tatcgaaaaa ctacgcagtt ttgcttctct tcatgaatgg ccactgcttg aagcagcgca
     1381 aaatgtggat ctggcaaata tcggtggaaa agcaggacag caattaggtg catttggaga
     1441 gatgatccaa gaagtcactc agatgatccc atacttgaca gtgacagaat tgacgaaaga
     1501 ggttttggat cgtagcggat atctagaaga cttaaaaatc caaaatacat tagaagcaca
     1561 agcacgcatc gaaaacttgg aggaattttt aacggtcaca caagaattcg acaaacaatt
     1621 cgaacagcaa aatgaagaag acgctgatgc accagaagaa aaactaacgg tcttcttaaa
     1681 tgatctggca cttgtttccg atattgacaa tttagaagaa gatgcttccc aagtgacatt
     1741 gatgactttg catgcagcca aaggattaga attcccagtt gtattcctga ttggtctaga
     1801 agagggggtt tttcctttat ctcgagcatt gatggaagaa agcgaactgg aagaagaacg
     1861 ccgtctcgca tatgtgggga tcacgcgagc agaagaagcg ttgtatttaa caaatgcttt
     1921 ctccagaaca ttatatggac ggacgcaata taaccgacct tctcgttttg tagaagaaat
     1981 cgatcaggag ttactggaaa tcgaaggcat gcgtcctacg cctaaaaaaa cacctgtatt
     2041 cgctaaaaaa acagcatata gctataaaca gccagagaca gcggttgttc cctctaaaag
     2101 tgctactgga ggggaaaata acagctggaa acctggagat aaagtcaaac acaaaaaatg
     2161 gggtctagga acagtcgtcc gagtaagcgg gacgtcgaag gatctagaac tagatgtggc
     2221 tttcccaagt caaggagtaa aacgtctttt ggctgccttt gcgccaattg aaaaagctta
     2281 gtagtggagg gtaaatgatg gaacatgaaa tgacgattga cgaagctgct aaacgagcag
     2341 aagaacttcg tacaagactg aaccaatggt ctcgcgaata ctacgtagaa gataaaccaa
     2401 cagtggaaga ctatgtttat gataaagaat acgcagaatt agtagcaatt gaggaacaat
     2461 atcctgattt gatcacgtct gattctccca ctcaacgagt aggcggaaaa gtattagaag
     2521 gttttgaaaa agtcactcat gatattccgc tttacagttt gaacgacgtt ttcagcaaag
     2581 aagagctgat agcttttgat caacgtgtac aaaaagcagt gggacgtgta gttgactatt
     2641 gctgtgaact aaaaatagat ggactttccg tttctttgcg ttatgaagat ggaaatttcg
     2701 tgagaggtgc gactcgcggg gatggaactg taggagaaaa catcactgaa aacctaaaaa
     2761 ctgtccggtc ggtcccaatc aaattaaaag aaccaatgaa catcgaagta agaggcgaat
     2821 gtttcatgcc aaaacggtca tttgtccaat tgaaccaaga cagagaagca gagggaaaag
     2881 atatctttgc gaatcctcgg aacgccgcag ctggttcttt gcgtcagtta gattctaaaa
     2941 tcacagcgaa aagaaatctg gacacttttt tatacacagt agctgatttc ggtcctatgc
     3001 aagcaaaaac ccagtatgat gcgctagaag agctagaaaa aattggattc cacacgaatc
     3061 gggaaaaacg tctatgtcat tcgattgatg aagtgtggtc atacattgaa gaatatcatg
     3121 acaaacgtgt ggatttgccc tatgaaatcg atggaatcgt catcaaagtg aatgaattct
     3181 cgctacaaga tcaacttggt tttactgtca aagcaccgag atgggcagca gcttacaagt
     3241 ttccgccaga agaagttgaa acgctaatcg aaaacatcga atggacagtc ggtcgaacag
     3301 gggtagtaac gccaacagct atcatgacac cagtccgagt agcaggaacg acagtaagcc
     3361 gtgccagcct gcataacggt gattatatta agttgaagga tatccgcttg aaagatacag
     3421 ttctgattta taaagcggga gacatcatcc cagaagtttc gcaagtcgtg ttagataaac
     3481 gacctaaaga cagcgaagaa taccaattgc ctacacattg tccagtttgt ggaagtgaac
     3541 ttgtccattt ggatgaagaa gtagccttac ggtgcatcaa tcctaaatgc cctgctcaaa
     3601 tgaaagaagg tttgaaccac tttgtttcaa gaaatgcgat gaatatcgat gggttggggc
     3661 cgcgcgtact agaacagatg tatgataaaa aactagtagc agatgttgct gatctttaca
     3721 aattgacaga agaagagtta ttgacgttgg acaaaataaa agaaaaatca gcaaataata
     3781 ttttgacagc aatcgacaac agcaaagata attcggtcga gcggttgatt ttcgggttag
     3841 gaatccgaca tgtcggagcg aaagcagcaa aaattttagc agaacatttc ggtgatctag
     3901 aaacattgag taaaagtgac tatgagtcga ttattgcgct tgatacgatc ggtgatatca
     3961 ttgccgacag tgtagtcact tatttcagta acgaagaagt ccatgaattg atgaatgaat
     4021 taaaacaagc aggcgtcaat ttcgaataca aagggcttcg aaatgcccaa ttgcaagaag
     4081 tcgagtctcc ttttaaagaa aaaacagtag tccttactgg taaactaaca cgatttactc
     4141 gtgaagaagc aaaagagacg atcgaaaatc ttggcgggaa agtgactggt agcgtatcga
     4201 aaaaaacgga tatcgtggta gcaggagaag atgctggcag caagctgacc aaagcacaag
     4261 aattaggaat cgaagtttgg acagaagatc aaatggcaga tgcattggct aaaagtcgct
     4321 cagtagaaga atgaaaaatg agttttttca gacaaatttc aataaacaat ttcagacacg
     4381 gcactcttgt gttaaaatag agagcaaagt ttttaactcg tttagaaaga aggggtagta
     4441 tggcgattag tgaagaacaa gtgaaacacg ttgccaaatt agcaaaactt gcgttcgcag
     4501 atgaagaact tgcaagtttt accgaccaat taggtaaaat catcgatatg gttgaaatgc
     4561 tggaagaagt agatacagaa ggtgttccat ttacgtcgaa tgtggttgaa acagtaaacg
     4621 tgatgcgaca agatcgggcg caagctggat ggaaccgcga tgaattattg aaaaatgtgc
     4681 cggaaacaga agacggcttt atcaaagtac ccgcaatcat cgataatggg gaggctggcg
     4741 cataatgaca gaattacaca atcgttctct agaagaactg catcatatgc ttgtatcgaa
     4801 agaaatcact gtacaagatt tgacaaaagc aacatttgaa cgaatcaaag aaacagagcc
     4861 tgaggtcgaa gcattcatta cattgaacga agaaaaagca ttggaacagg caaaagcttt
     4921 agatgaaaaa gggattgatg aaagcaacgt ccttgctggt attccaattg gtatcaagga
     4981 taatatcgta acaaaagata tcttgacaac cgctgcatct aagatccttt acaattttga
     5041 accaatttat gatgcaacgg tcatggataa aatctatcaa gcagatatga tccctgttgg
     5101 taagctaaac atggatgaat ttgctatggg tggaagtaca gaaacttctt atttcaagaa
     5161 aacaaaaaat gcttgggacc caacaaaagt acctggtggc tcttctggtg gttctgcagc
     5221 agcagttgca gctggacaaa ttccagtttc tttaggtagt gatactgggg gaagtatccg
     5281 ccaaccagca gcttttaacg gcatcgtagg gatgaaacct acttatggcc gtgtctcacg
     5341 ttttggtttg attgcctttg cttcatctct tgaccaaatc ggaccaatga cccgtacggt
     5401 gaaagataat gcattagcat tgacagctat cagcggttat gacgaaaaag acggtacttc
     5461 ttcaggcgtt tcagttcctg attttgcaga gggactgaca ggcgacatca aaggaatgaa
     5521 gattgcttta cctaaagaat acttaggcga aggtgtggcc cctggcgtaa aagaagctgt
     5581 attgaaagca gcagaaacat tccgttcatt aggtgctgtt gtggaagaag tcagcttgcc
     5641 tcattcaaaa tacggtgtgg ctgtttatta tatcattgcc tcatcagaag caagttccaa
     5701 cttgcaacga ttcgatggta tccgctacgg ttatcgctca gaaaatgtga aatcattaga
     5761 tgatgtatat gtcaactcac gttcagaagg attcggtgaa gaagtaaaac gccgtatcat
     5821 gttaggtaca ttctccttaa gtgctggtta ttatgacgct tacttcaaaa aagcgggaca
     5881 agtccgtaca ttgatcaaac gagattttga aaaagtcttt gcagattatg acttgatcat
     5941 cgggccatct tcaccaacag ttgcattcgg aatcggtgag aacattaacg atccaatcac
     6001 aatgtacatg agtgacatct taacgatccc tgtcaacttg gctggacttc caggtatgtc
     6061 tgtaccggca ggtttatcag aaggattacc agttggattg cagatcatcg gtaattattt
     6121 tgatgaaaaa acgatgtatc aagcggcata tgcgtttgaa caagcaacag aattccataa
     6181 acaacaacca gctattttag gagggaaaga cgaatgaatt ttgaaacagt cattggacta
     6241 gaagtccacg tcgagttaaa aaccaactca aaaattttct ctccagcgcc agctcatttt
     6301 ggtgcagaac caaacagcaa tacaaacgtg atcgactggg gttatccagg cgtgctgcca
     6361 gtaatgaata aagctgcgct ggaattcggg atgaaagcag ctttggcatt aaactgtgaa
     6421 atctctaaag atactcattt tgaccggaaa aattactttt acccagataa tccaaaagca
     6481 tatcaaattt cccaatttga tcaaccaatc ggtcatgacg gatggatcga aatcgaagta
     6541 gaaggtaaaa agaaaaaaat ccggatcgaa cgtgtccatt tagaagaaga tgctggaaaa
     6601 aacatgcatg gtattggcgg ttattcttac gttgatttga accgacaagg cacgcctttg
     6661 atcgaaatcg tttctgaagc tgatatgcgt tcaccagaag aagcatacgc ttatctagaa
     6721 gcattgcgat caattatcca atttacagaa gtcagcgatg tgaaaatgga agaaggctct
     6781 atgcgctgtg atgccaatat ttctcttcgc ccttatggac aagaagagtt cggtacgaaa
     6841 gcagaattga aaaacttgaa ctctttatct ttcgtgaaaa aaggtttagc ttttgaagaa
     6901 aaacgtcaag caaaagttct tctttcagga ggagaaatcc aacaagaaac acgccgtttt
     6961 gacgaaacaa cgaataaaac agttttgatg cgtgtcaaag aagggtctag tgattatcgt
     7021 tacttcccag agcctgatat cccgaaattc gtgatcgatg acgaatggat tcaaaaagta
     7081 aaagctagtt tgcctgagat gccagcttct cgtcgcgaac gctatatccg tgagtttgga
     7141 cttccagagt atgacgcaat ggtattgaca cttacaaaag aaatgtctga tttttttgaa
     7201 gctgttttgg aaaatggtgc agatgccaag caagcatcta actggttgat gggtgaagtt
     7261 tctgcttact tgaacagtga gaaactggaa ttagctgaga caaaattgac acctgagaac
     7321 ttagcaggaa tgattcgttt gatcgccgat ggaacgatca gctcaaaaat cgccaaaaaa
     7381 gtcttccgtg aattgatcac aaatggcgga gaagcccaag aagtagttga aaaaaatggt
     7441 tgggtgcaat tatctgatcc gaacaaacta ctgccaatca ttaatgaaat tttggataac
     7501 aaccaacaat ctgtcgatga tttcaaaaat ggaaaagatc gtgcggttgg cttcctagtc
     7561 ggtcaaatca tgaaagcaac taaaggacaa gcaaatcctg gtgtcgtgaa taagctactt
     7621 aaagatgaat tagcgaaacg atagaggctg aaaatgaaaa aagcacgcgt aatttataac
     7681 cccacctcgg gaaaagaatt attgaaaaaa aatttggcgg atatcctaca ggcattagaa
     7741 gaagcaggat ttgaagccag cgcctatgca actacaccag aaccagattc agccaagaat
     7801 gaagcatgta gagtcgcaga actaggtttt gatctagtag tggcagctgg tggcgatggc
     7861 acaatcaacg aagtggttaa tggaatcgct ccgcttgaac accgtcctaa aatggccatc
     7921 atcccagctg gtacaacgaa cgactatgct cgagcattaa aaattcctag agataatatt
     7981 aaagcagcag cagaagtgat aaaaaaacac caaaccgtaa aaatggacat cggcctttct
     8041 gataacagtt acttcatcaa tatcgctgca ggtggctatt tgacagaatt gacttatgaa
     8101 gttccatcag aactaaagag tatttttggt tatcttgctt atcttgcgaa aggagcagag
     8161 atgctccctc gagtaaagcc aatcaaaatg cgcttgaagt atgatgaagg ggaatatatc
     8221 ggaaatgctt cgatgttttt cctagggctg acgaactctg ttggaggatt cgaacagatc
     8281 gcgccagatg ccaaattgga tgatgggaag ttttctttaa tcattgtgaa gacagcaaat
     8341 atctttgaga tcttgcatat tgcagcactc atgctgaatg gcggtcgaca tgtcgatgat
     8401 ccaagaataa tctataccaa aacaagcagt ttgttcgttg aaacagaaga tgatatcaat
     8461 cgaccagtaa tgatcaactt agatggtgaa tacggcgggg aagcaccgat gcatttccaa
     8521 aatcttcatc aacacattga gttctttgcc aataccgatc agattcctga tgaagcaatc
     8581 acaggtacag atgaagaaga attagaggtt gtcagcaaag aattcgtaaa agaagtcgaa
     8641 cgtttaacgg atgaagatat cgacggtgac ggaaaaatag cagacgaaac acctaatcaa
     8701 aagaattaat caaaagccgg tgctgaacgt agtaaggtca gtgccggttt tagttgtgta
     8761 attagctgat ttatagtaga gttcagtagg attgaaaaat aggcttaaag gagcaaaata
     8821 tgacagaaca gcaaatagta aagaaaaacg aacgatatac agtcgaaatc gtcgacttga
     8881 gttatgaagg aatgggggta gcaaaaatcg atggttatcc attatttatc gaaaatgcac
     8941 tccctggaga agaagtagaa atactagtag taaaagcagg caataagttt ggatatggaa
     9001 aagtcgaaac atttattaca caatcacctg atcgtcagcc tgtagaaaac aatgcattac
     9061 ttcgcatagg tatcgcacct ttagcccatt tgaagtatga acaacagctt gtcttcaaac
     9121 aaaaacaagt agagcatgta atggcgaaga ttgctaagat gccagaagtt cctgtacttc
     9181 caacggtcgg aatggcagaa ccattcggct accgtaataa agcacagatc cctgttcgcc
     9241 gtatcgatgg acagttagcc accggttttt acaaaaagaa tagccatgat ctagtcccta
     9301 tcgaagattt ttatatccaa gatcctgtca tcgatcaagc aatcaaagta gtcagagata
     9361 tcttgcaacg gttccaagta cgaggatata atgaagaaaa aaatgaagga cagcttcgtc
     9421 acatcattat ccgtcgtggt cattattccc atgaaatgat ggtcgtttta gttactcgta
     9481 aagaaaaatt ctttaaatcc aaggaaattg ctgaagctat tcatgaagaa ttgccagaag
     9541 tggtttcggt catccaaaat atcaatgaag aaaaaacaaa tgtgatttta ggagacaaag
     9601 aaaaagtttt atatggcaga gactatatcg aagatcagct attaggaaaa acctatcgaa
     9661 tctcagcaaa gtcattttat caagtaaata ctcaacaagc agagtatctg taccaaacag
     9721 ccatcgagtt tgccgagtta gataaaaaag acattgtgat cgacgcttat tcaggaatag
     9781 gtacgattgg cttgtcttta gcagacagag tggataaggt gtatgggatg gaagttattc
     9841 cagaagcaat tgaagatgca cagttcaatg ctttaaataa taaaatcgaa aatgttcatt
     9901 atgaagtcgg aaaagcagaa aaagttatga aaaaatggca agaaaaaggg atcaaaccat
     9961 ctgtgatcgt agttgaccct ccacgaaaag gattagatca tcattttatt gaatcagcaa
    10021 tcgaaatgtc accagaacga atcgtctata tctcatgtaa tccggcaact tttgcaagag
    10081 atgctaagtt atttgcagaa gcaggttatg ccgtgaagaa agttcagcct gtagatcttt
    10141 tcccgcaaac ccatcacatc gagtgtgtag gactgcttac acgtgtggaa cgttgatatg
    10201 acaaggatta gagcgattcg ggatggtttc cggattgctc ttttttcgtg ttgaaatgac
    10261 gatttttggg tgagcgtaga gttgatggaa atgctatgga gctttgagtt gataggagtt
    10321 acaccgtaga cttgataggt tagatgattt tgataacggt tacgtgatat aatatattat
    10381 cgagttttca agggagacaa acttatgaag caaattttta tattttcaga tacacagaaa
    10441 gataatattt cttattttga acgcgagcca gcaatcttgg tttcaatgag tcgtagtgat
    10501 ttgccgtatt tgaaggaaca ggatttttca aattatccag ctgtttatgt tttgattggt
    10561 ggtaataagc gttacgtggg tcaggcagca gggcaatcaa tttcacttcg tttatctcaa
    10621 cactttctca aggaatataa agcttgggtt gaatcagttt tattctttac aagagcagat
    10681 gggaaaatgt caaaagcaga tacagattat cttgaaaaac gacttatcca agatttccaa
    10741 gaaaaatctg actatgaaat gatgaattca actgctggta atacttcgta tattgataaa
    10801 cttcaaaaag caaaatcgga tcagctatat gacacagtgt ttgaaattat agatgaaatt
    10861 gcaaatatcg atttgtttgg tacttccgaa gacagtgaaa cccaaattta tgaatttagt
    10921 tcatctggac aatatgaaat tgagtatgat gggaaaaaga tatcttcgaa atctgctaga
    10981 ggtttattgg ttgattttgt ttctcagcta tttgcggatg gaaactacaa caatcaactg
    11041 caagaattaa ttgtcgatga gacgccgtcg ttcgctataa ttttaggacg taaaccatcc
    11101 atgtataacg gaagaccaaa ttcagccagg gtagctgatg gagtatgggt ttatgcaaat
    11161 ttcaacaaaa agaatgtcag aatcaaaatt gataaactag ctcgtcaatt ggggattccg
    11221 gttaaaataa agtggaatta atatttgtta tctcgccttt agtaaatcta tgctgaaggc
    11281 ttttatttta gaaaaatagt ttgcgttcgc gaaaatattt catgtttcgt ataataatga
    11341 tagctcatag atgatataat tgaagatacc agtagaatag aggtgtagta tgtcaatttc
    11401 ttataataaa ttgtggaagt tattgattga tttgaatatg aacaaaactc aacttcgtga
    11461 aatatctggc gtcagtccca atgtaatagc aaaactcggt aaaaatgagt tggttgcaat
    11521 ggagtcatta ttgaaaattg ctgctgcctt aaatgttgat gttagtgaaa taattagttc
    11581 agatgttata gatgcggagg atgttaaata aatggcaaaa aataaagttt taaacatcac
    11641 tgaaattgaa gatggtgtaa aaaaaatact ttcaaatagg ggcgatgatt tcatcactca
    11701 atttttgtcc ttgtacgata tccccaaaac gtcgattaca cgtgcaaaat caaaattcga
    11761 taatggtgaa ccatttgtga ttaagaacaa acttcattat gaagaaattg aaggcaacgt
    11821 tgtcattgcg attgatgcga ttgctcaatc aatcaaagag caaaaatcta aaccacgata
    11881 tatcttggta aacgattatt tagatattgc tgcaattgat acgaaaactc atgaaacttt
    11941 gaatattcca attgaagagc taccggctaa tgctgacttt ttcttagcgt ggaatggcat
    12001 tgagaaggct gattatcaag ctgaaaatcc agctgaccga aaagctgctg aacgttttgc
    12061 aaaattgtat gatgtggtgg ctaaagataa tcctgatgcc aacgagcatg catttaactt
    12121 gttcttgatt cgagtgctgt tcttgttgtt tgcagaagat acaggaatta tgaataaggg
    12181 ttcctttaca aatgttctaa aaacaagaac ggctgaagat ggttcgaatt ttaacgaagt
    12241 aatcaaagaa ctctttgcaa tcttggatat taatgagtta gctcgcaatg gcaaggatga
    12301 gtggctactt gaatttccat atgttaatgg aaaactgttt ggtgagccac atgtggattt
    12361 gaccttttca aaagtttcaa gagagctgct tattgaagca ggggaattat tgaattggaa
    12421 tgaaatcaat cctgatatcc ttggcgcaat gattcaatca gttgcttcag cagaagaccg
    12481 tcacgttgcc ggtatgcatt atacaagcgt gccgaatatc atgaaagtca tcaagccgtt
    12541 attcttagat gatttgacag agtcatttga aactttaaag cttcgcagtg aagaaaacga
    12601 gctgaaggac attactgaaa aaacaagaaa cgataataag agaacaatca tcaatgatct
    12661 aagtgctttg ttgacgcgaa tttctaatat taagtttttg gatcccgcca gtggttctgg
    12721 taattttctg atcattgctt ataaggaaat ccgtagactt gaaattaaga ttttgcaatt
    12781 gttgggcgac ttacaaaata atgattccat gccactatca gtgattcatc ttggtaattt
    12841 taatgggatt gaattggatg attttgcgca tgaagtagca agactttcat tatggattgc
    12901 tgaacatcaa atgaataaag agcttgaaga agcaatacca ggtaccattg ctgagttgtt
    12961 accgttaaaa gatgctggga atatagtgtg tgccaattct cttcgaattg attggaatga
    13021 ggtgttgcga ctcaaagaaa atcatgaaat atatataatg ggaaatccac cgtatctggg
    13081 caaaagtctt caaaatgctg aacaacgtga agatatggaa aaacttgttg gcgatattcg
    13141 aaaatataaa ttgcttgatt atatttttgg gtggttttgg aaagctaaac aatatattgc
    13201 aacatctcat ggctcgttcg catttgttac tactaattca atttgtcaag gtgaacaagt
    13261 tagtatcctt tggccgtacc ttttaaaaga tgaggtaaga atttcatttg catatcagag
    13321 ctttaagtgg aacaacaatg caaagaataa tgccggtgtg actgttgtca ttattggtat
    13381 gacaaataat gtgaatattc atcctaagtt atatttagag aatgggtcag taatgagtgt
    13441 ttcaaatatt aatgcatatt tagctaatgg tgaaaatgta tttgtcaacg aatcaagaaa
    13501 tagtatttca aatttaccct ctttgaaatt tggcagtatg gcaaatgatg gtggtaatct
    13561 agttttttca gtggcagaat actggaattt gattcaaaaa tatccagata ttaaacattt
    13621 atttaagaaa tatttaggat caacaggatt aataaatgga gacttaagat atactttatg
    13681 gttgaccgag gaagatgcaa ctagatacaa acaaaattct cttattaaag aaacaataaa
    13741 aaatgtaaaa agttataggg aaaaaagcaa gagaaaagaa actcgagcac tagctgagaa
    13801 gccatggtca tttggtgaag ttcgttatga tgaaaccagt gagaatagta ttctaattcc
    13861 tcgtgtgtca tcagaaaaaa gagattatgt tcctatgggt atagttggga gcgatactat
    13921 aatttcagat tcggctatgg caatctataa cgctccaatg tggctgcttg gtctgttaga
    13981 atcccgaatg catatggtgt ggctgcgctc tgtcggtggg aaattagaaa cttggtaccg
    14041 ttattcagcc gggttagtct ataatacttt tccgattcag gatttatccg cccagcgtaa
    14101 aaatgaaatg actcgcgtga tgacagaaat tcttgattta cgagaatacg aaggcggcac
    14161 gttagccgat ttgtataata aagacagtat gccagaatca ttacgtaaga aacaccagga
    14221 actggatggg attgttgata gagcttatca gcaacgcccg tttgaatctg acgaagatcg
    14281 acttggaaca ttactaaaac tctatcagga gatgacaatc aatgactagt aatttatttg
    14341 aaattaattt tgataaaaat caatcaaaaa caacaaatga cctcggtatg cgtgaaatgc
    14401 aagaacgtgt ttatgaaaag cgtgcgtcac agtatttgtt ggtaaaatca ccaccagctt
    14461 caggaaaatc acgtgcattg atgtttgttg gcttggataa actccataat caaggaatta
    14521 aaaaagtggt tattgcagtt cctgaacgct caattggtaa atcatttaaa gatactgaat
    14581 tatcgaagtt tggtttttat tgggattggg aagtaaagcc aagtgacaac ttaactattg
    14641 ggggcggtga aaaatcaaag gttcaacact ttgttaaatt catgaactcg actgacccaa
    14701 gtgataatat cctgattgcc acacatgcta cgttgcgatt tgcttttgaa gagttggacg
    14761 attcattatt tgacaatacg ctattagcga ttgatgagtt ccatcatgtg agccgtgatg
    14821 ataaatctgt gttaggtaat gccttacgta gcatcatgtc aaactctaat gcgcatatct
    14881 tagcaatgac tggttcatat ttccgtggcg actctgttca gattctggaa ccagccgacg
    14941 aagataaatt tgataaggtc acttacactt attatgagca actggaagga tataaatatc
    15001 tgaaatcctt tgcaatgggg tatagtttct atcgtggaaa atatacagat gctttggata
    15061 aagtaattga tgtcaacaaa aaatcaatta tccatattcc taatgttaat tctggtgagt
    15121 ctactaaaga taaatacgcg gaagttgatg aaatacttga tttattgacg gacggtggta
    15181 atatcacaca gaacgaggat ggtatttatg aaatcgaacg tccagatggt aaaaaactat
    15241 tagtggccga ccttgtgaat gaagaaggac gtgaaaaagt ctcagcatat ctagcaagta
    15301 ttacggatga cattaaagat cttgataagc ttgatatcat cattgcactt ggaatggcaa
    15361 aagaaggctt cgattggcca tttgcggaat atgcactgac aatcggttat cgtaactcat
    15421 taactgaagt gattcaaatc attggtcgcg taactcgtga cagccccaat aagactcatg
    15481 cacagtttac aaatcttatt gcgcagccag atgcacagga tgacgaagta ttattcgcgg
    15541 ttaacagtat tatgaaggca atttctgcat ctctacttat ggaacaagtg ttggcgccag
    15601 tttacaagtt taaaccaaaa gatggtaagc cagacaaacc aggtgatctt tcaatcagag
    15661 gactgaaacc tgctgatacg acggaacgta caaagaaaat catcgaaaat gatatggaag
    15721 atttgaaatc tagcatttta cagagccaag atattcaaaa tgcgattgtt tcaggtgctg
    15781 atgcgaagat gattaataag acgttgattc cacgtgtcat cattgatcgt tatcctgatt
    15841 tgacgaacga agagttagaa accgttcgtc aacatacagt tgccgatata aatattgcgg
    15901 ctggacggac aacaacttca gaagatggta gccgcgaaat tattaagatg gccgatcgtt
    15961 tcattaacat tgatgaattg aatattgatt taatcgatca agtgaatccg ttccaacgtg
    16021 cgtatgaagt gattagtcgt gatattgatg caccgactct tcggtttatt caggattatt
    16081 tgtcatcaaa taagtatgat tttactgacg aacaattggt tgcggcctac caagctgcta
    16141 agaagttccg tgcagaaaat ggtcgaaatc ctgatcgaaa tagtaaagat gatggtgaaa
    16201 aatacttggc ctttgctttg ctacgacttg ctgagataaa acgtgaaaga gaggcagcga
    16261 aagaagatgg cgaatagttt agatagcatc tttgaagatg acgcttttga tgatttaacc
    16321 aagcaagttg aaaaaaagaa agttgtaaaa caagacccag aagttgctaa atttcaggag
    16381 attatagact ttgtgaatga aaacggacga gagccagaaa aaactcctga atggtctagt
    16441 gaacgcgcat tgtgggctcg tcttagcgga tttcgaggaa aacccgaaag gtctgagaag
    16501 gttaagaagt atgacactct tggtttattg gagaaagaat tcgaaccgga tggagagttt
    16561 ctcgctgatt cagttgaaat cgatgacgag ttgaatttag atgatatctt aggctcagat
    16621 gatatgattg atgatttttc agacttgttg gatgtttccc gctatcgtag aactgtgaac
    16681 gctgcagata aaattggccg cagaaaacat gcaagtcatt ttgaaaagta taagagcttg
    16741 ttcgatcaag tgcatgcaga tatcgcatct ggcagacgaa aaattatccc gtttgagcag
    16801 tatgatattc atgaaaatag attctacatc caaaacggcg taatgatgta tatcgtttcc
    16861 atttcgaagg atacctttat cgctgataat gggaaaacaa atgccagaat gcatgttgtt
    16921 tatgagaatg ggacagagaa taagaatctg ttgttacaat ctttagcttc gtctctatac
    16981 tcgactgaac gtcatggaag gatggtgaca gagccggtag atgaggaaac actggctgaa
    17041 agctttggag aagagtttac gacaggttat atctacgtgc tgaagtcatt aagcacgaat
    17101 ccggatatta gcaagtggaa taacctgtat aagattggtt tcactcgaaa tgatgttgaa
    17161 agtagaattg ccaatgcgga aaacgacgtg acctacttaa atgcaccagt gaaggtcatg
    17221 ctttctgctg aagtacaaaa tgtcaacgct cagctacttg agcgcactct ccatcatgta
    17281 ttccaggaca aacaagttgt ctttcaggat ggaaacttca aaaaagctac tgaatggtat
    17341 atggttccat tgaatgatat tgaatcgaaa atcaatgaag taatcgctgg attacaaaaa
    17401 taaatatgaa aatgcactgt actcatgaat cgaatacagt gcatttttga tatttaatcg
    17461 cgaacgattt caacaatatc cgataaatca caatcaagtg cttcacagat gcgaagtaag
    17521 atatctgttg ttacattttc gcctttattt agttttgaga gtgttgctga actgatatca
    17581 gtcatttttc tgagttgtgc tttattcagt cctctgtcga ttaggagttt ccaaagcgga
    17641 ttgtagctaa acttcatgta atcattcctg ttctagttat aaatatcgta tagatatcca
    17701 tagcgtccat tgtttttatc ttcagtaaag acaatctgat ttacttgttt atcatcatag
    17761 tttagtgctg gcaagttcgt tgtattctca acgatgataa gttgtccttc gttttgatga
    17821 tccataaagt atttgaaaag tccttctttc atattgtcat cgccatcttg ttttttgcgt
    17881 tcatctaatc caagaagagg ggaatcgata atgaacacag aaggtgtgta cttagcgtga
    17941 ttatacaaga gatgtcgata tgccattacc actactgaat tgataaacgc tgtatatcca
    18001 tgtccgtttt cggtccattt tagatttcca ttgattttga tgtcgaaaat atccttggcg
    18061 aaaaatgcgt tacgaaattc attaggttta taatgcatat tacgtaacat ggagtccagt
    18121 aactcagaca tttctttgta gaattctgca tcaaatttct ctttaggttt ataaaggata
    18181 cgttcgtctt ctggctcatt ctcgattttt tcaagatcac ttttaagctt attttgcatt
    18241 ttttccatcg agattagctg ttgtttaata tccctgtaag tttcgatttt attgattcga
    18301 tcctttaacg tctcagcttt cggttttagt tcatcgttga ttgctgcatc aagcgatttc
    18361 tttcttgttt taagctcatc aagctgttcc attaactctt gcagttcaac ttcggtatca
    18421 tgagattctt ccgtcaaatc tgataattga gagagcaatg aatgtaattc tgacctagca
    18481 gtatcgatgt aggttgtttg gacaggttta cttagttcgc cgttacagaa tggacagcta
    18541 tgtgaagtat catgttcatt atgatcattt aataatcgct caccatctac gattagtgtt
    18601 agtcgttcaa tatccgattg atattgataa tgtaactgtt tgtacctatt caaaagaagt
    18661 tgagtttcat gaagatactg ttccttttga taaatctctt ttatcaagat tttgctgctg
    18721 gctaacgcat cattgatttg cttttcaatt gtttcaagtt cagcgaacat tccatcgaca
    18781 atttgttgaa aatcatcaga gggagcctca ggtaattctg atttagccac ttcgtagttt
    18841 tcatataatt cagaaaggct tgaattgatg tagttaatca ccgagttcct tctaattttt
    18901 ttatgttctt ttgtttcgtt ttcttccgag ttcgcaaaat ctgaaccgta tagcagaagt
    18961 aacaacgatg ataaaaaagc tgtgcgttgg gttgtttccc caggaaaaag aacagaggcg
    19021 ctggtattga ctctatcttg tggaataagt gtgctaggcg ctaatgtccg ccaagttaat
    19081 ttttgacgtt tgaaatcctc attcttgata atctgataat catcatcgat gcctaaaagt
    19141 tgtaaccaaa aatggctaat tggatttttc ccagatcgtg cattatattt ttcataatct
    19201 acatattcac ctgaactcgt tattttggat accaaaatgg agttgctatc aaattctcta
    19261 gttaatgaat atttgccatt agtcgattga ataatcagtt caactttact gtatccggtt
    19321 gcatcggttg aaaagggagc agcgtctttt ttcgcgccat agagaaaatc gattgccgta
    19381 acaatgctag tttttccggt atttgaaggg ccagaaatta tgtttagtcc attggtgaac
    19441 ttgacagttg catcttcacg atctttgcct tttatggtaa cacgttcaat ataataattt
    19501 tgcattatca ttgctctcct ttaaattcct ttgcgcgttg gtttatttgt cgcagaagct
    19561 caacgtcact tttatcttga atgtatttaa cagattcgtc agctatttgt aagtattttt
    19621 ctgcatatac cgatacgaat tgtgatatca actttaggcc atcttctgat aaagcatact
    19681 taaaaccttc tttgctttgc gatggttgga taacatgttg cagtacaaga tttttgactg
    19741 catctttaac taattcacgt cgcgcaataa attcactgaa tctatagctg ttatcaccat
    19801 ttaagttgta atcggaaatt tcaaagtcgc gaccatacgt ggtgatgaag tctgtgatta
    19861 aaattctatc agcgctgaca ggctctttaa attcagagag cagtaacatt actcgaaccg
    19921 aaatttcaaa tggagtatta aacaaactat tcatcttcag tcacccattt gatctttccg
    19981 tcattaacca gcatgtggca aatgcctttt ttagcagaat tttgcagcaa tcccggtata
    20041 aacgataaga ttgatccgtt taatgttata acagacgact gctgtagaac ggctttgagt
    20101 ctctcaacgc catcatcgta gtcttcttca tagacttcct cgatgccgtc gtacatatct
    20161 tcttttaaag cttcaaagtg ctcagttcct tcctcttgtg taaagttatc acggacccca
    20221 cgacgtatgc tttcagcttt atagtaattt cttcgctgtc tcttgaaatc ctttagccgc
    20281 gaatttggca ggtcgtttgt atgctcgtat acttcgtatc catcggcatc tgcatatgct
    20341 gaaaaaagag ctatgacata cgtcatctcc tcagctctaa tatcaagggg aactggcaaa
    20401 tcgtcaaaga attttttcac ttcatcatta taagagaaat caccattagt gttgattctt
    20461 aaacttgcga gctctaattc gctaagcttc ggcttggcct tttttgtttt tacttttgtt
    20521 tgagaagcaa ttgaggtgat ttcatcaagt aaaagatggg ccactatttc agaaacgtct
    20581 gtatctttgg ggattaccac acctttatca atcaattgtt gcttaaggac ggttggagca
    20641 tcatctataa gacgttcata aataaaagtc gtgcatttgt cgatgttgaa ttttccagca
    20701 acccgaactg catactcttg tgttaatggt cgagtaccat tgtaaaatct tcgtaggtta
    20761 tcatcagaaa aaccgaaaat ggtggcctcg tcatcatttg aaacactatc cagtaaagca
    20821 attaaaaaat cctgtggctt cacgtactcg cttcccataa acggataaag cgtttgagta
    20881 atttcactta tttccaaaat acaacctcct ttgttacgtc cgccgttgac cgccgtttgc
    20941 cgccgatgac tgacgaaaac aagggaggcc tttttctata attaaaacat agtagtttaa
    21001 ctatttaatt ttatcattta tggttcataa acactacatt atgtttcaag aaagtgaacg
    21061 aatttatctt aaaagcgaat agtcctacta ataaaatata acaatgcctg agttgcaata
    21121 gggcaaggtt acaaatactg aaaagaactg gttatccaga aaatttttca gatattgtat
    21181 gccttacttt attgcgctct tttttcgtga tgagggacgg tgtacgtatt ggcacggtcc
    21241 ctgtttgttt tccttgctca aaagctcttt caggcaagaa agagggatct aatgagtaga
    21301 tttatgaaaa cacaccaaaa cgaccgcagc acttacaagt acgtgtttga gcgtactgat
    21361 gccaatggac gtatccaaaa agaagtggtt gagattcgcc caggcgaaga cggcgtcact
    21421 gagcttaaca ttcaggagct gcatcgcgct gacgaccgtg cgatttacaa cttttataaa
    21481 aacgcacgtc ctgaacgcac agaagaagaa aaagaaaaga tcaaggcttg gaaagagaag
    21541 ttccgtgaag actttaataa tgagcacggc tattatccaa tggacgaata cgtggatggt
    21601 gaagccaacg attacttcaa acgtaactac aatttgtcgc tcaatgaatt tgatgatgaa
    21661 tgcgatgagg gggacaaccc catccaatca ttggtttacg atgcttacca agataatgaa
    21721 gaagatcctc gtatcgatcg tttgcgtgaa gtcgtgacgc aattattacc gtaccaccaa
    21781 gaaatctatc acatgctcgt cactgaaaac atgtctcaga ctgaagtggc ggacaagctt
    21841 gacaagaaaa aacagtcaat caatcgcacc tttggaatca tcaaagacaa gatcaaagaa
    21901 ttattttaga aaaaagttac gggggagttt actttccccg ttttccttcg cctgtattgt
    21961 gtaagggagt agatcctaaa caaataaaga tgaaggagga ccatcatgaa cctaaaacac
    22021 aaaatccgag taaacgtgac tgatgacacg ggtaacaaag aaactgtcct acaaggtggt
    22081 gtgcgtcatt tgcctcgctg gtttgcacgg tggctgttcg gtaagggcaa catggacaaa
    22141 attctcgtgc tcacaccagg ggacacaatc gaatcagtcg aaatccgtga agttaacaag
    22201 aagggagcgt aatgacgtga gtaaaacaaa actatttctt caggtcgttg aggacgtcca
    22261 agcgttagct gatagcttac aagagttggc tgtggcgatg aaaaccaatg aacctgaacc
    22321 acaagttgaa acaccagaac cgacaatgga aacaaaatca gaagtcaaac aaccgacgct
    22381 tgaagaagtc cgtggtttgt tggcacgtaa atctcaagaa ggtaagtcaa ctgaagtgaa
    22441 agcactgatt gagaaatatg gtgccagtcg cttgagtgat gtgccgacag aaaactacgc
    22501 ggctttggtt gcagatgcgg agggattgta gatgccaaca caacacgcag tcctatccgc
    22561 atcttcaagt aatcggtgga ttcactgtcc gccaagtgtt cgcttgtcag agcactttga
    22621 aaacaagtcg agcccgtatg cccaacaagg cactgatgct cataccttat gtgagtacaa
    22681 actgaataag ttcattggta atgacgtcac agacccaaca tctacgcttg aattctacga
    22741 cgaggagatg gagcagtgtg ctgaaagtta tgcgaccttc gtcatggaag aagtcgctaa
    22801 ggcgaaacag acaacagttg actctattgt aatagtagaa cagcggctcg acttctcaag
    22861 gtacgtccca gacggctttg ggaccggcga ctgtctcgtc attgcggatg gaaccctatc
    22921 tgttattgac atgaaatacg gtctaggaat tttggtagat gcgtatcaga acccacagat
    22981 gatgtgttat gccttgggag cgttggagct tttcgatgtt atttatgaca ttcaagaagt
    23041 caagatgacc attttccagc caaggcgtga gaatgtgagt acgtacacac tgctgaaaga
    23101 agaattactt cagtgggcaa aagatgtgtt gtcgcctgcc gcagatttag ctttcaaagg
    23161 tgagggtgag taccaatgcg ggaaatggtg tggattctgt ccggcgaaga atagctgtcg
    23221 tgcacgagca gaccataact tgaaactggc gcaatatgag ttcaaaccac cagagcttct
    23281 tagtgatgat gaaatcgaag aaatcatcgg taaagtggat gacctggttt cttggagtaa
    23341 cgatatcaag gactgtgctt tgaaacttgc actcggtggc aaacaatgga ctcatcacaa
    23401 attagtcgag ggtcgctcta ctcgtaagta ttctaatgac aatgatgtcg ctgcggcggt
    23461 catcaaggct ggatacgacc catatgataa aaagttactt ggtgtcacag caatgacgaa
    23521 ggcactcggt aaagagaaat tcaatgaaat cttgggcgaa tacatcatca aaccaaaagg
    23581 caagctgacg ctagtcgatg gttcagataa acgacaagcg gtcacagtaa acaacttaaa
    23641 cgaagaattt aaaccattaa cggaggaaca ataatatgtc taaaacaagc aaagtaatca
    23701 ctggtacaaa cacacgtctc tcatacttcc acggttggga accagcatcc atcaacgggg
    23761 gcgcagagaa atattcaaca gcagtcttga ttccaaaatc agataagaga acgattgata
    23821 ccattgaaaa agcaatcgat gcagcaatcg aagagggact tgcgaaattc ggtgggaaga
    23881 aaccaaacaa agcagaaatc aagctgccat tacgtgatgg ggatatcgaa cgcgacgatg
    23941 aagcatacca aggccattac ttcattaatg cgaactcaac cactgcacca caaatcgtgg
    24001 atcagcacgt tcaaccaatc ttggatcgtt cacaagtgta tagtggttgc tacgctcgtg
    24061 tgtcgattaa tttctatgcc tttaacgcaa atggaaatcg cggggtagcg gcagggcttg
    24121 ggaatatcca gtttgtacgt gacggcgaac cactcggctc acaagtatct gcaagcgatg
    24181 agtttgaaac cttggcggat gatgatttct tggcttaatc attaaaacaa aaacggccat
    24241 ggcagaagtg ctgtcgcctg tttgattgga ggaagaaaat gaaacatttg tcattggatt
    24301 tggaaacgta tagtagtgtc gatttatcga aatgtggtgt ctacaaatat tcagagtccc
    24361 ccgattttga aatactgcta tttgcataca gtgtagatgg tggtcccgtc gaagtggttg
    24421 atctgaagat ggacgaaact atcccggctg aaatattaac ttcgctatca gatgaaacag
    24481 tgattaaaca cgccttcaat gcgagttttg aacgtgtgtg tttgtcacgc tttctggggt
    24541 tggatggctt tttgaatcca cgagggtggc aatgcacaat ggtgtggggt gcgacattag
    24601 gtttgccgtt gtcacttgta ggagttggtg ctgttcttgg tttggataaa caaaagctca
    24661 cagaaggaaa agacctcatc cgatactttt gcgtgccatg caatccgacc aaagttaatg
    24721 gtggacgcac gaggaactta ccacaccatg cgccaactaa atggtcagca tttaagacat
    24781 acaatagtcg tgatgtggaa gttgaaatag ctatccaagg aaaactttcc aattttccag
    24841 tgcttgattc tgtttggaaa gaatactggc tggatcagga aatcaacgac cgaggaattg
    24901 agattgataa ggactttgtg gaagctgccg ttcagttgga tgaattatca aaagctggat
    24961 tgaatcaaca attgcaagaa ttaactggac tagaaaatcc aaacagcgtg cttcaaatgc
    25021 ggggatggtt actagaccac ggacttgaaa ccgagtcact cggtaaaaag gcagtggccg
    25081 aactactcga acaacctatt gatgaaaatt taaagcaagt cctggtctta cgtcagcaac
    25141 tcgccaagtc atccgtgaaa aagtatcaag ccatgcaaaa ctttgcgtgt tctgataatc
    25201 gttctcgtgg tttattccaa ttctatggag ccaaccgtac cggacgcttt gcaggacgtg
    25261 gcatccagct tcaaaacttg cctcagaacc atatgtcaga tttatctgaa gcgcgtagtt
    25321 tagtaaaaca aagcaatttt gaaatgctta acattctcta cggcaatgtc ccgaacgtac
    25381 tctcagaact cctccggaca gcgtttgtac cacgtgaagg gatgaaattt atcgtatctg
    25441 acttctcagc gattgaagca cgtgtgattg cttggctgtc gggtgagaag tggcgtcaag
    25501 aagtattcaa aaatggtggc gatatttact gtgcgtctgc cagtcagatg ttcggtgtgc
    25561 cagttgaaaa gcatggcgtg aatgggcatc tacgtcaaaa agggaaaatt gctgagcttg
    25621 ctctagggta cggaggatcg gtgggtgcat tgaaagcaat ggatgctctt gaaatgggac
    25681 taactgagga tgaattacag ccgcttgtta attcttggcg agactcaaac attaacataa
    25741 cggagctttg gtgggctgtt gataaggcag taaagacttg tgtgaaagaa cgacggagca
    25801 cagcgacgca tggcatcaag tttgaataca agtcgggttt tctgttcatc caactcttgt
    25861 caggacgtaa attagtttat gtaaaaccaa agattggtga gaacaaattc ggcggtgaag
    25921 ctgttaccta tgaaggtgtc ggtgctacca agaaatggga acgtcttgaa agctatggtc
    25981 ccaagtttgt cgaaaacatc gtgcaggcaa tagctagaga cattctctgt ttcgcaatgg
    26041 agaatcttcg taagtatcgc attgtcgggc acgtccacga cgaagtgatt atcgaagtgc
    26101 caatggaatc gaaggttgaa gacatctgtc agttgatgag tcaaacgcct aagtgggctg
    26161 aaggcttgct actgaacgct gatggatacg agtgtgaatt ctatcaaaag gattagtcaa
    26221 tgtggagtca gaaatggctc catatttttt tcaaattttt ttatcaaaaa taaggttcga
    26281 attgggttct ttcttcgctt atagacgaag gcaaaaaaat caaaaataaa ttcgagtcag
    26341 agtttacttt cgaccatttt cttcgcctgt aagttagaag gggcaaaatg cctctcctga
    26401 aaattctatt acaggaggtt cacaagatga acgaattgaa agtttttgaa aatgcggagt
    26461 ttggctctgt acgcacggtg atgattggcg atgtccctca ctttgttggt aaagatgtag
    26521 catcaattct tggctacaca aatccacaaa aagcattacg agatcatgtt gatgaggaag
    26581 ataagacggt gaacgaatcg ttcagcgtta atgggacaat ggctatcctt atcaacgaaa
    26641 gtggattgta ctcactcatc atttctagca agatgccgaa cgcgaagaaa ttcaagcgtt
    26701 gggtgacaaa tgaggtcttg ccgtctatcc gccaacacgg tgtgtacgcg acagaagatt
    26761 tcatcacaaa gtcaatcgaa gatccagcat gggccatttc agtgttgaag cagctacaag
    26821 agaaaaaaga aatggtggcg atgcaacaac aaatgatttt ggagatgaaa ccaaaagtta
    26881 gctactacga tttgattctt caaaacaatt ctgtgatgtc gatttctaaa atctcgaagg
    26941 actacggtat gtcaccacag gcaatgaaca agttgcttca tgaactggga attcagtaca
    27001 agcaaggcaa gatgtggttg ctttatcaga agtatgccaa tcaaggctac acacaatcaa
    27061 aaactcatgc gatagatgca gagcgcagcg tgatgcatac ctactggacg cagaaaggtc
    27121 gtttgttctt gtacgacttg ctgaaatcga agaagaacat cttaccagtg attgagcgag
    27181 gtgataataa tgcctagacg taaagattac agaacatcag tctatgtctg ctcgccgttc
    27241 tcaggaaatg ttgtcgtcaa ccataaaaac actaagcgtt actgccgtat tgctcttgac
    27301 cgtggctaca tgccagttgc ggcacatctg atgtatccac aattcatgga cgacgcaacg
    27361 gagcgtgatg tggcaatgca catgtcactt gttctcatgg gcaaatgtga ggaagtctgg
    27421 gtggtgggtg acaccatctc ccaaggcatg gcagttgaaa ttgaacaagc ggagtattgg
    27481 aagaaacata tcaggtactt cgatgatgag atgaaggagg tctcaaatgc ttaagatgac
    27541 gttattcaca tccaatcagg tgggtaattc caagaatgga ttgttcccaa ccaagcaagt
    27601 ggtgacagat aaagaaacac ttgttgccac ggtcaagctg gatcatgtga tggctgagta
    27661 caaaggcaac tatcgcaaag gtgacaactt catccagtct gatgtgattc cacttgattg
    27721 cgacaatgac cactcagaca atccagatga ttgggtagcc cctttcgaag tggcgatgca
    27781 gtttcctgat gtgacgtttg cggtcagcta ttcacgtaac aacatgaaag acaaaggtgg
    27841 caaatcagca cgtccacgtt ttcacgtata tttcccgatt cctgccacaa ctgataaaga
    27901 tgaatatgtg gaactcaaga agcagattca aagtgagttc ccatactttg acaacaatgc
    27961 gatggatgcg gcacgtttgc ttttcggaac accaaactct gaagttgaaa tctatgaagg
    28021 tagtaagttg atcactgatt ttctcgaaga tgatctgttt gctgattggg ataatgcgca
    28081 gtcagagatt agcgaaggca gccgtaattc aagcatgagt cacatcgcag gaaagctcat
    28141 caaacgctac ggtgcaacgg aagatacgta tcacaagtat ctcgaacaat cccaaaaatg
    28201 taatccgcca ctgccggagg aggagcttaa agctatctgg aatagcgcgg tcaactttgg
    28261 gaagaaagtg aaacaacaag aaggctacat tcctccagag cagtacaact ctgatttatc
    28321 acttgagcca accgactact cggacttagg tcaagcaacg gtcttagctc gcgagtatga
    28381 atgtaagctt cattactcac cgtcaactgg ttaccttgtc tacaacggca gctattggga
    28441 agagtcgaaa ccaaaagcgc aaggtgtgat ccacgcattg acagaacgtc aactggaaga
    28501 atctgaaacc gaaattgaga agcgtaccaa ggaaatggtt agtaatggcg cgttcgatgt
    28561 acttgctagt gttggtccga aaaaggcagt caccatgttc aacacagcgc aacgtcatag
    28621 ttttgatttg taccaacacg cacaagagta taagaagttt gctgtcaaac gccgcgacag
    28681 caagtatctc tcgtcagcat taaccgaggc aaagcccatg cttgaaatcg agcaacggtt
    28741 actagatgtg aacgagttct tgctgaacac accaacagca acctttgatt tgcgaacagg
    28801 caaatcacaa gaccacaact cagaagatta catcacgaag caaacggaat gtgccccgag
    28861 tgacgccaat cagcagattt ggttggacgc tctgaacacc attttcgtag gtgaccaaga
    28921 gttgattgat tatgtccaga tgattgtagg acttgcagct attggtaaag tttacgttga
    28981 agcgcttatc atctcgtatg gtgaagggcg taacgggaag tccacgttct ggaatgtcat
    29041 cagccgtgtg ctcggtaatt actctggcag catctcagct gatattctta caagtcaaat
    29101 ccgacgcaat gtgaaacctg aacttgcgga agcgaagggg aaacgcctac tcatcgcagc
    29161 cgagcttgaa gaggggatgc gactcaacac atctaacatt aagcagctct gctcgacgga
    29221 tgaaattgcg gctgagaaga aatacaaaga cccattcaag tatgtcccaa cgcatacttt
    29281 ggtgctctat accaaccacc tcccaaaagt tggagcgatt gataaaggaa cctggcgtcg
    29341 tttaattgtg attccattcc ttgctacgat tgacggcaat aaggacatca agaattatgc
    29401 ggactacctt tttgaaaatg ccggcggagc cattttgcaa tggatccttg acggtgcgaa
    29461 aaaagtcatc gccgcagact ttcatttaac ggttccacaa gttgtcgcta atgcaatttc
    29521 tgaatataag acggcgaatg attggcttgg ccatttctta gacgagtgct gcgagactgc
    29581 tgttgacttt gaagaaaaat ctggtgagtt gtatgcagag tatcgtgcgt tttgtacgcg
    29641 aactggtgag tacattcgta gttcagccga tttttacacg gctctggagg cgggcggatt
    29701 taagcgccgg cgaactaata aaggaaacaa gattgttggt ttgcaactca aatccgagtt
    29761 cttggaatag ttgattttaa aggaaagtgt aacctgtgta ggtcttatat agaacttttc
    29821 ttaagggata aaaaattacc acctatataa agttatgtat tgcgtattca cgggttacac
    29881 catacttgca gattggagaa tatctaaatg aaatttaaac agtggttaca aattggaatc
    29941 ggtaaacgtg atttacgatt tgatgagttt tacgagtttg ccatgtcgga tgtcacgtat
    30001 ccttggaaga aatcattcga taagcaagtc gcacatctga tgattcactg cgcagaaaag
    30061 gaatacttgg aaatcttgcg tgatgcgtat ttcgagtatt tgacggtgtg gctatgagag
    30121 aaaagcaggt tgagcaagca ctagtgaaag ccgtcaaaag ggtaggtggt atttgcccga
    30181 agtttacatc accggggctt gctggtgtgc ctgaccgatt ggtacttatg ccaaatggga
    30241 aacttggatt tgtcgaagtg aaggcgccag ggaagaagcc acggtcttta cagctttttc
    30301 gtatgaaaca actgacagat ttagggtttc agtgtttcgt gcttgatgag attgatcaga
    30361 tacctgagtt actagaaaga ataggaggtg atgtctgatg aagttcgtac cacataacta
    30421 ccagaaatat gcgattgatt ttatcaaaga tcataaaact gccgcccttc tattggacat
    30481 gggcttgggt aaaacggtga caacgctgac agctattaaa gacttgatgc atgatgactt
    30541 tacaatacaa cgtgtcttaa ttattgcacc gttgcgcgtg acgcaatcaa catggccaac
    30601 tgaaattcaa aagtgggatc atttgaaaga tttatcgtat tccgtggttc ttggaacacc
    30661 aagtcagcga atggcagcat tacaaaataa tgccaacatc tatctgatca atcgtgagaa
    30721 cttggactgg ctgattaaca aatcgggttt tacgtttgat tttgatatgg tggtgattga
    30781 cgaactgtcg tccttcaaaa acttaaaagc taaacggttc actagcttta tgaaagtcag
    30841 acacaaggtt gaccgaattg ttggattgac cggaacacct tcaagtaatg gtttgatgga
    30901 tttgtttgcg gagttcaagg ttctggatat gggcgagaga ttgggctatt atatttcacg
    30961 ttatcgagat aaatactttc tacctgacaa gcaaaatggc atgcagattt attcttggaa
    31021 accaagggaa gatgcagaac aagaaatcta cgacaagatt tcagatatca cgatttcaat
    31081 gaagtcagtc gatttcttgg atatgccaga gcttgtgatg aatgaagtgc cagttgatat
    31141 gggactcaaa gaaaagcaaa agtacgatga aattaaggca gacttggtgc tacaattgaa
    31201 agaagccacc attgatgcag ccaatacagc ggtgctttcg aataaactgc tacaaatggc
    31261 gaatggagca atctatgatg agtttaatgt gagtcatcac atccatgacc agaagttaga
    31321 tgctttggaa gatttgattg aaggtacaaa tggcaaaccg attctgattg cctattggtt
    31381 ccagcatgat ctggagcgta tcaaggaacg attcaacgtt cgacagatca aaacgtctca
    31441 ggacattatt gattggaacg aaggtagcat ccccatcgct gtcattcatc cggctagtgc
    31501 aggacatgga ttgaatttac aagctggtgg ttcaacactt gtatggtttg gtcttacgtg
    31561 gagtttggaa ctttaccaac aaaccaatgc aaggctgtgg cgacaagggc aaagtgatac
    31621 tgtggtcatt catcacatta ttgcgaaaga tacgattgat gaagatgtga tgttggcttt
    31681 gaaacgaaaa gataaaacac aatcatgttt gattgatgca gtgaaagcta gattggaggg
    31741 aagcaagtga caccaaaaga atacttgaaa caagcgtacc gtttgaataa acgaattgac
    31801 gaccggatta aagagaaggc ggagctctat caactggcgg tgacagtttc ttccacaaca
    31861 ttcgaagaaa agatttcaag tggcacacgg aatactgagg caccatttgt tcgtaacctt
    31921 gagaagtgtt accaattgga agctgaaatt gatgcagaga ttgataagtt tgtagacctc
    31981 aaacgacaaa tgcgtggcgt gattgatgaa ctcacaaacg tggacgaaaa gattgtgtta
    32041 atcaatcgat acctgttgga agagagctgg gacaagattg ctgaagatct tcacgctgac
    32101 gtgcggacag tctttcgttg gcatagcaaa gctcttgaga acttgaagct accagatgaa
    32161 cccatagtca tttgaaatgt gtcagtgaat gtcagtagcc tttagtgata tcattaagat
    32221 gtcaaaaaag acaaacgata ctcaagcaat accagcaata gaagtatcga acaaatactc
    32281 gaagcctttg tgggaaaaat ccttcaaggg cttttctttt gcccgaaagg atgtgacccc
    32341 acttgccaat gaaaccagct aacccctgta agttcccagg ttgtccgaac cgtacgcatg
    32401 atacttattg tgatgaacat aaaacacttg cgaggaaacg ttacgagaaa tacgagcgtg
    32461 atccggcaat caacaaacgt tacggcagag cgtggaagaa aatacgggca aggtacgtgg
    32521 cagcacatcc cctatgtgag atgtgtctga aggaaggacg ctttacttcg gttgagattg
    32581 tgcatcacat caaggaatta agtgatggtg gtacgcatga tgaaaataat ttgatgagtg
    32641 tctgcaaatc atgtcactcg aaaattcaca tgactcgacg aaacacaaaa gataaaacaa
    32701 tcatgtgaaa aataacaagg gggggagggg cggtaattat ccctaaaccc ttctaaactg
    32761 tacagcgcgc taggccctta cgcgaaaaaa gttcagttca aacgggggat agccccaaac
    32821 aagaaaaaga ggtgataaat tttggcaaaa gatggaacac aacgcggtgg cagacgacct
    32881 cgttcagggg aacgctcaca gccactcgtt gataaagttg ctgctggaaa gaaagcatcg
    32941 cggatggaat ttgacttacc ggatgttgaa ttattaattg gtgaagatat tggtgagggt
    33001 gtagagcttg agggcaatga ttatcctgaa ccaagtgctt acctttctgc aaaacagaag
    33061 gatggcactg tgctaggcgc tgatgaaatt tataactcaa cgttccaatg gttgaaagac
    33121 cgcggatgcg aaacgttgat agccccacga atggttgagt cctacgctca gaactttgcg
    33181 cgttttgttc aatgtgaaaa tgctctgagt caatatggtt tgctcggaaa acacccgact
    33241 accaaggcag tcatggcctc cccatttgtg gcgatgtcgc agaacttcca aaagcaagca
    33301 aatgtgacgt ggtatgagat tcaacagatt gtaaaagaga atgcatcagt ggaatacaca
    33361 ggagatccga atacggatgc gatggaacga ttgttacgtt ctaagaatgg ttgaaaggaa
    33421 cagaatttat gatagagaag gttaatccgc aacatccaga taaagttgca gatcgtattg
    33481 ccggagccat tgtggacttg gcatacgcag aagaaaataa tccaaagata gcagttgagg
    33541 ttttaattgg tcatggcatc tgtcatgtga ttattgaaac ttcaactgct ttaaatttga
    33601 atgaaatcag ccaagcgatt aatcgtatcg caggcaaaat taaaaaagat gtggtgattg
    33661 tgccccaaga taaagagttg gctgacaatc aagcgactga aattcgttgt ggcgataatg
    33721 gaatctttcg tgggatgcca ttgactgatg aacagttaca gctctcaaaa attgcgcgtg
    33781 acttatatca ggaccatcca tttgatggaa agtacattct tgatggtgaa cgcttgataa
    33841 tttgtcagag ccacgcaaag acaaatgagt taaaacaaaa atatccaaac gctacagtca
    33901 accctctagg tgattggact ggcggcccgg atgtggatac aggtgcgact aatcgcaagc
    33961 ttggttccga tatggcagac ggagtaacag gtggcggtat ccatgggaaa gacttatcaa
    34021 aagctgatgt gtcagtgaac atctacgcat tcttgaaagc tcaagaaact ggtatgcccg
    34081 ttgagttaag ctgtgcgatt ggtgacactg agattgatgg cgtgccttat tctgaaatcg
    34141 tggaagttgc gcgtgattat gtgaatgagc ttggcgggtt tgagaaacta gcagaatggg
    34201 ggttgttcta atgaacacaa cagaacgttt tgagaaagtt gatattgata agctggtccc
    34261 ttatgccaga aatgcccgta cccacagtaa ggatcaagtg aaacagattc aagcttctat
    34321 gcgtgagttt gggttcataa atccggtgat tgttgataag gattataact tgattgcagg
    34381 tcacggacgt attcttgcgg ctaaagaaga aggtatcaag caggtaccgt gcgtgtttgt
    34441 ggaacaccta accgaggcac aaaagcgtgc gtacatactt gctgataacc gactagcaat
    34501 gaacgccggt tgggatgatg aaatgttggc ggttgagtta tcagacctgc aaggtgaaga
    34561 gtttgatttg gatttgcttg gttttgaaac tgatgaactt gataaattac tcaacggtaa
    34621 tgatgatgcc caagatgatg actttgatgt agatgaggaa cttgaaaagc cagccttcag
    34681 taaaacaggg gacttgtggt tgcttggtcg tcaccgttta gtttgtggtg actcaacaaa
    34741 acctgaaact tatgagaaac tcatggacgg caaactagca aatcttgtgg tgacagatcc
    34801 accttatggg gtctcatatc aaggtaaagc gggaacgatt aaaaatgata atctgaagga
    34861 cgaggcattt tacaacttct tgtatgatgc cttttcttgt atggaaagtg tgatggccaa
    34921 cgacgccagt atttatgttt tccatgcgga tacggaaggc ttgaacttca gaaaagcatt
    34981 cgtggatgca gggttctatt tatcaggtac gtgcatctgg aagaaacaga gtctagtgct
    35041 tggtcgttcg ccttaccaat ggcagcatga gccggtgctc tatggctgga agaagaaagg
    35101 caaacatcaa tggtacaccg gacgtaagga atcgaccatc tgggaatttg ataaaccaaa
    35161 gaagaatggt gaccacccaa cgatgaaacc aattccgctg ttggcatatc cgattaccaa
    35221 ttcgtcactc agcaactgca tcgtgttgga tagttttggt ggcagcggat caactttgat
    35281 tgcttgcgaa cagaccgacc ggatttgcca tacgatcgaa ctggatgaaa agttctgtga
    35341 tgtgattgtt aagcgttata tcgagcaggc tggaagcgat gcagacgttt cagtgattcg
    35401 agatggaaag actatcgcat ttaatgacct agaaaatcct gaataaataa cgtatcactt
    35461 gctataaaat ccctttagag tgatatatag actaccaaaa gaatacggaa gacaaagtta
    35521 tgtttccaac tcatgaacaa atcgagggta tgaaaaaacg ttaccctgaa ggtagtcgtg
    35581 tagaactcat caagatgaac gacccacatg ccccagcgaa gggcacacaa ggcacagtaa
    35641 ccggtgttga cgacattggt tcaatcctta tccaatggga caacggttca acactgaacg
    35701 cggtatacgg cgaagatgtg gtgcacatca tcaaaccaga cttcgaattg gtctttcaaa
    35761 acggtagaag agaaggattt gaaaccttca atgatgcttg gcaatacgtg acggacatga
    35821 tattgaacca cgatttggtc tgggttgacc ttgagtcaaa aacagaagat accatctaca
    35881 tcagaaaggg gctttaagat gaaagtgagt aaacgtaaga tttacaacat cgcaaagaat
    35941 tacatcgttc agttgcctga acgtggcgac ctgaaggccc acaacagcga ccgtgaagac
    36001 ttcttggaca ttgcggtttg gtgtttggaa gatgccttaa ttgcggccta tgaggcaggc
    36061 agaaaggacg gtcaaaatga acgcaaagat taagcaacaa atcattgaga ttgcagaaag
    36121 tggtctgaca aatatgtttg atgcgattgc agtgcagcga attgcacatg accggcactt
    36181 ttacgagttg gtgattttca ttgaagaaca caaagatgag tacagccgct ttatcttgga
    36241 gggtgataaa tgatggatta tcaaatttac atgccagata atgaagatgg cattgaagaa
    36301 ggatactacg attggcaaga actggtgcaa ttgctgcggg ataacaagaa caaccctgaa
    36361 gccattcagt tcattgccga tatgatggaa taaagagatt aaggcagctg acaacagctg
    36421 ctttttgcgt agaagggagt gagttccaat cgataattac aaaccaactg aatttatgga
    36481 caaagactcc cattatgata agtcacgtgc agattatgcc gtggccttta ttgagttgtt
    36541 gaaacacacg aaaggtcagt ggttcgggaa accgttcaaa ctgatgcctt ggcaagaaaa
    36601 gattatccgt gacttattcg gaacgattaa ggcaaatggc tacagacaat tcaatacggc
    36661 ttatgttgaa atacctaaga agaatggtaa gagtgagtta gcggctgccg tggctttgct
    36721 tttgacttgc ggtgatgggg aagaacgcgc tgaggtatat ggctgtgctg ctgatcgagc
    36781 gcaggcttca atcgtatttg acgtagctgt tgatatgatt cgccagaacc cagcgttaag
    36841 caaacgagtg aaaatcctga cttcacaaaa acgaattatt tatcagccaa cgaatagttt
    36901 ctatcaagta ttatcagctg aagcatactc taaacacgga ttaaacagtt ctgcggttgt
    36961 atttgatgag cttcacgcac aacctaacag aaagctgttt gatgtcatga cgaaaggggc
    37021 aggggatgct agaacgcaac cgctattctt tttgattaca acggcaggga atgataccaa
    37081 ttcaatttgt tatgagcagc atcaaaaagc tctcgacatt attgaaggtc gaaagcatga
    37141 taaaacattc tatccgttaa tttacggtat caagcaagat gatgattgga ctgatccaga
    37201 agtttggaag aaagccaatc cgtcacttgg gataacgatt gcagaagaaa aggtggtggc
    37261 tgcttgtgat tcggctaaac aaaatccagc tgaagaaaac tcattcagac agcttcgcct
    37321 gaaccaatgg gtgaaacaag cagtgcgttg gatgccaatg gataaatggg acgcttgttc
    37381 attcaatgca gatgaagaaa gtttgagagg tcgagtttgt tacggaggtc tcgacctttc
    37441 cagcacgacc gaccttacat ccttcgtttt ggtgtttcca cctgaagatg aggatgacaa
    37501 gtttgtgata ctgccatatt actgggtgcc agaagatacc ttggacttac gagttaaacg
    37561 cgaccatgtg aattatgatt tgtgggccaa gcaggggtac atacaaacca ctgagggaaa
    37621 cgtagtccac tatggtttca ttgaaaaatt tattgaggaa ctgggtgaga aatacaatat
    37681 ccgggagatt gcctttgacc gttggggagc agttcaaatg gttcaaaact tagaaggtat
    37741 gggatttact gtagttcctt tcggtcaagg tttcaaggat atgagcccgg ctactaaaga
    37801 gttgatgaag ttgactcttg aacagaaatt agcccatggc ggtcatccgg ttcttcgttg
    37861 gaatatggac aacattttcg tccgaactga cccggcaggt aatatcaaag ctgataaaga
    37921 aaaatccact gagaagattg atggtgccat cgcgacaatt atggctctgg atcgtgcaat
    37981 tcgttgtgga aatgataatg gtgccagtgt ttatgatgat aggggattgt tgtttctgtg
    38041 aaatatgagc ttttattaca atccattgat ataattgtct ttggaggtgt atgtgtatga
    38101 atgaatattt gattcaagta gcaccagata aaatatcttc agacccggac aacacattaa
    38161 tttctagact gttttataga aaatggtggg gaaaaattct aactgaagat ttgacaaagt
    38221 atgctgaagg aattgggaat tataggggag aaacgattat ttctttcaac acaattgctg
    38281 gtgcaacgat acggttattg aaatgctata atcaaaagaa cttttcaatg ccagatagtc
    38341 attatgggcg tttgaaagtc attttatatt ccgatgatat caccgaagag atgaaagtga
    38401 aatttgtaaa attctatgat ttaagtcata ctcttgcaaa ttttatgcca ctatatgtat
    38461 caaaaccttc tctcaatggt gttaaaggta aagggccgta tcatgacttt cctgatttat
    38521 tttattctga tgtaagagat aaacgatatg gttcatcaac taacgctgat aaatttatcg
    38581 ttaataataa agactatttc tctaaattca aagattggaa aagttttata gaactaaatt
    38641 atttacaaga tttcttcgat gatgaagatt atatgacgtt taaaaaactt gcaccaagta
    38701 gttcattctt tccttatcaa agaaaatcct taacacctga cgaaaaatcg aaatgtttag
    38761 aggaagtaaa taccttttta aataatgcaa ttgagataat tttgtcaaga ggaagtcggt
    38821 tttatatcta atgtgaacac agcatctaca aatcgtaggt gcttttttga tactcaaatt
    38881 ttgaaaggag caatcaatgg gcataataaa atcactattc aaatcacgcg ataaaccaac
    38941 aaatcaaacc atcatgaatc cataccgatt tatgtttggc ggctcatcag ctggtaaaag
    39001 cgtgaccgaa caaacagcta tgcagatgac ggctgtgtac tcatgtgtgc gcgtgctatc
    39061 agaagccgtg gcaggattgc cattacactt ttaccaatac cgagatgctg gcggcaaaga
    39121 taaagcaatg aaacattcgt tgtatttctt gctgcatgat gaaccaaatt cggaaatgac
    39181 atcatttgta tttcgtgaaa ccttaatgac tcacctgcta ctttggggta acgcctatgc
    39241 gcaaattatc cgaaatggca aaggtgaagt tgtagctttg tatcctttaa tgccagataa
    39301 gatgacggtt aatcgcgatg acaacaagga aatctattat ttatatcaag ctgatgtcgg
    39361 gcaagtcaga ctttcaaaag ctgacgtgct tcacattcct ggccttggct ttgatggctt
    39421 ggttggttat tcaccaattg ccatggctaa gaatgctatc gggcttgcga ttgcaacaga
    39481 ggaatatggt tctaaatttt ttgccaatgg tgccaatcct ggtggtgtcc tagaacatcc
    39541 tggaactttg aaagacccag gaaagcttcg tgaaagttgg aatgcgacat ttggtggcag
    39601 ttcaaatgcg gccaaggttg cagtacttga agaaggcatg aagtatacgc ctatttccat
    39661 gtcaccagaa caagcccagt tccttgaaac acgtaagttt caactggaag aaatcgcacg
    39721 gatattccga gtgccccttc acatggttgg cgacttagac cacgcgacct tctcaaacgt
    39781 tgaacatttg agtttggatt ttgtgaagtt ctcattatca ccatggctca atcgttggga
    39841 acaagcactt aataaatcac tgttaagtcg cgatgaaaaa gaaaagtatt tcataaagtt
    39901 taatgtggat ggcttattgc gtggtgacta tgccacacgt caacaaggct atgcaaccgg
    39961 tattcaaaat gggttcctat ctcctgatga tgtgcgtgaa cttgaggact tgaacccaat
    40021 ccctggtggt gctggtgcga aatattactt caatggcaat atgctgccaa ttgaacaagc
    40081 cggggcagct tatggcgtaa acaaagaaga aagcgaggaa caagatgaag aagttttgga
    40141 agtggaagaa tcaaacggtt ctgaatcaag acaaccaaga ggaaatccta gagagaaccc
    40201 tctacctcaa cggaccaatc gctgaggaga gctggtttga tgatgaagtc acaccgcaga
    40261 ttttcaaaga tgagttaaat tcaggtggag gtaacgttac agtctggatt aattcgggtg
    40321 gtggcgattg tgtcgctgcc gcaagtattt acaacatgct tgctgactac aaaggtaaag
    40381 tcacagtcaa gattgacggc atcgccgctt cggccgcaag tgttattgca atggcaggtg
    40441 acaaggtact catgagtcct gtggcgatgg tgatgattca caatccgtca gcggttgcgt
    40501 ttggcgaaaa agttgatatg gagaaagcca ttgccatgct tgatgaagtg aaagcaagta
    40561 ttatcaacgc ctatgaacgt aaaacgaatt tatcgcgtgt gcaactggca catatgatgg
    40621 atgcggagac gtggatggac gcaaataaag ctgttcacct aggatttgcc gatgaaatca
    40681 tcggtgagat tgacccggat attccggtta ataattcgat gtactcggaa gtcaaagtaa
    40741 ctaattcact gacttcagcg attgctaaga agtgtcgtat tgaaccacct gaaccaaagg
    40801 aacgagaaga aattaataac actatcccag ccgattcgtt aatgagtcgg cttaatttaa
    40861 tgaaaaattg gaggaactaa cccatgaaca aaattcaaga attgatggaa aaacgtgcac
    40921 aagcttggaa cggagcaaag gcttttgtag aagcaaagca agataaagat ggcttgatgt
    40981 ctgaagaaga tgccaagact tatgcggaaa tggaaacaaa agtgaagaac tttacgatgg
    41041 aaattgaacg tatgcagtca atggaagcga tggatcgtga acttgaaaag ccaatgagcc
    41101 aaccattaac ttcgcaacct atgactactg aaaataaaat ggaacctaag aaaacaggac
    41161 gtgcttcagc tgaatataaa gaaggtgtac ttgaagcctt gcgctcaaac ttccgacatg
    41221 tttcaaatgt tcttcaagaa ggtgtggata cggcaggcgg ttatttggtg ccagaagaat
    41281 atgactcacg tttgattgac ggcatgactt ctgaaaacat catgcgtaca cttgcgacaa
    41341 caattacgac aagtggtgaa cacaagatta atattgcagg ttctaaacca gccgcttcat
    41401 ggattgaaga aggtggcgag ttgagctttg gggatgcaac gttcgatcaa atcttgcttg
    41461 atgctcacaa acttcatgtg gcaatcaagg tgactgatga attgctttat gacaacgcct
    41521 ttaaccttga aaactatatc atcgaccaat tcaccaaagc cttagcaaac gcggaagaag
    41581 atgcgttctt gaatggtgac ggtgttggta aaccactcgg tatttttgca gcaacaggtg
    41641 gcggacaagt tgccattaca actaatactc aaagtgcagt tacggcagat gatgtggtga
    41701 accttgtgta tgctttgaaa cgtccatatc gtaaaaacgc gaagtttatc atgaacgacc
    41761 aaaccatcag cttgcttcgt aagttgaaag acaacaatgg tgcctacatg tggcagccag
    41821 ctttggtagc aggtgaacct gaccgtctgc tcggttatcc agtttatact tcggagtttg
    41881 ttccaaccgt taaggcaggt gcgccagtca ttgcttttgg tgatttcagc tactataaca
    41941 ttggcgaccg tggcgtgcgt tcattcgacc agttgcgtga actctttgca ggtaatggca
    42001 tggttggttt cttagctaaa gaacgtgtgg acggtaaact tgtgttacct gaagcagtgc
    42061 aaatcttgaa gatgaaagca tctgcctcat cttaaggagt agcctatgag tgacttacta
    42121 aataaggtca aggcaaacct catacttgag cataacgaag atgatgagct gttggaacga
    42181 ttgattctga ccgctctttc ttatgctgag agctatcaac atattgagtc tggctactat
    42241 tcagaacatg aaatgccgcc aaccactgaa caggcggtta ttgtgttagc cagtaacttt
    42301 tacgaaagcc gtgatggttc aactggtggc ttctttgcgg ataacgtcaa tgccagtaag
    42361 caagtctggg aaacggtcaa tctgttactc aggctcgata ggcggtggca agtatgagtt
    42421 ttggaaagat gaatcaacga atacaaatcg ttaaagtgac gaaccaaaag gatagcgctg
    42481 gattttcagt gaaacaagat gaagttcttg cagctgtcca tgcatacaaa gaggaaaaga
    42541 atgcgactga gaaatgggta aacatggcga cttttagttc agcgacctgc ctgtttcgga
    42601 ttcgttatat tcctaatcta gaggttacga cagagatggt tgtcatttca gatcagaaaa
    42661 ggtatcagat tgtgtcggtg gagaatgtcc gaaatcgcaa tatgtacctt gaaatgctga
    42721 ctactgaaat ggaggtgagt gcggatggct aaagcattga tgaagatgcc agaagatttc
    42781 ttgttaaagg tttcaaaact agaaaacaag acagatgaaa tcatccccaa ggttttagaa
    42841 agtggtgctg aagtggtgga aggcaaagtc cgaagtaact taactgccgt tgttggctca
    42901 aatacaaaag taccaagtcg ttcaactggt gaacttgaat cagcccttgg tatcacacaa
    42961 gctcgtcaag ataaaagcgg caactggaac atcaaggttg gttttgatga gcctcgaatc
    43021 gatggcagtt caaatgctaa gattgcgaac attctcgaat atggaaaaca cggtcagcca
    43081 gcaaagccat tcttgaaacc tgcaaaatca caaagtcgca agtcatgcat tgaaacaatg
    43141 aaatcaaaaa tggaaagtga ggtaaacggc atttgaacat tttagaggaa ttgaacagtc
    43201 tttttgccac acttcatatt cctgttgaaa cgggcgagtt tagtgataaa gcgccagaca
    43261 agtattgtgt gctgacacca ctcgctgaca ggttcgatgt ttacggggat aactttccaa
    43321 ccgttgatgt acaggaagtt cgcatttcct tgtttgtgaa aggcaactat ctgaacacgc
    43381 aaaaacaact ggttcagaac ttgttgaaac acgagtttac gattactgaa cgattatttg
    43441 taggatttga aaaagataca aattactatc atgtggcgat tgatgtcgcc aagcattatg
    43501 aaatggaggt ctgaaaatgg caacgattgg attggataaa ttatattatg caccgattac
    43561 agagtcggca actactggcg aagaaacata cggcacaccc gctatgcttg cgaaagcaat
    43621 ctcagctgag ctttcggtgg aacttgctga agcaacttta tacgccgatg atggtgcatc
    43681 agaagtagtg aaagaattta aaaatggaaa gttaacgctc ggtgttgatg atattggacg
    43741 tgctgtggcg gcagtgcttg ttggtgcaac cgtggatcaa aacggtgtct taatttcgac
    43801 aaccgaagat ggtggcacac cagttgcgat tggtttccgt gctcgtaaat cgaatggcaa
    43861 atacaaatat ttgtggttgt accgagtgaa gtttgccgtg cccagcacaa gcctagcaac
    43921 aaaaggtgac gacattacat tctcaacacc aacaattgag ggtacagtca tgcgtcgtaa
    43981 caagttagat gctagtggta agcatccatg gaaagcagaa gttgatgaaa acgatacggg
    44041 agttgaacaa acaactatca atggatggta cacgagtgtt tatgaaccag atttcacagc
    44101 agactaggag gcaacaatga gtgaagaacg cagtgccaag attagccttg gcggagatga
    44161 atatgaactg attttgacaa caaaagccac gaaagaaatc gccagtcact acggtgggct
    44221 tgataacctt ggtgagaaat tgttgaaatc cgagaacttt gaattggcgc ttgatgaaat
    44281 catctggttg attacgctgt tggcgaatca gtccatcaag atttacaact tgaagaacac
    44341 agaaaagaaa gatgaactca cgactgaata tgtggagttg atgacgagtc cgttgcagtt
    44401 ggctgaatat aaatcagcaa tcactgaagc aatgttcaag ggcacagctc gcaatatcga
    44461 aagtgaaaca gattcaaaaa acaaagcagg cgagtaagtg atgaagaaac atttactcgc
    44521 ctgtattact atgggacagt tcaaatgggt atgacgtcag atgatttctg gtactgctcg
    44581 cttggactgt ttttggattt atgggagtgt cataagcaat ttactgggat tagcaagccg
    44641 aaagttgagt tattcattga tgatgtgata ccgagtggaa tttaagggaa ggtggtgatt
    44701 tatttggcag ataattttgg tttaaagatt ggcgttgaag gtgaaaagga gtttaagaac
    44761 tcgttgcgtg aaatcaacca atcattcaag gtgctaggtt cagaaatgaa actggtatca
    44821 tccgaatttg ataaaaacga taaaagcatc agctcactgt ctgctcgcaa ccaagtctta
    44881 aacaaatcga ttgatgccca gaaagaaaaa atcaccaccc ttgaaaatgc tttgaagaac
    44941 gctagtgaca gttttggtga aaacgataag cgtactcaga attggacagt gcaattaaac
    45001 aatgcaaaag cccaactcaa taatatggag cgtgaagttg ctaacaacga gaaggcaatt
    45061 gacgccatgg gtactgaaga agttgatgct gctaagaaaa ctgatgagct tggcaacgaa
    45121 ctcaaggata caggtgacga ggctgaaaag tctggaagta aatttgaaaa gttcggtggc
    45181 gttctaaaag gtatcggggt agcaatgggt acagtggcac ttgctgctgg cgctgctgcc
    45241 gtggagctag gaaaacaagt ggtatctgct tacgcggact atgaacaatt agtcggtggg
    45301 gtggatactc ttttcaagga tgcgtcatcc accgttcaaa agaatgccga caatgctttc
    45361 aaaactgccg gaatgtcagc caatgaatac atggaaactg tcacaggttt ctcagctgcc
    45421 ttgatttcat ctctcggtgg tgataccaag aaagccgcag gagttgcgga tcaagctgtt
    45481 actgatatga gcgataatgc aaacaagatg gggacggata ttgagtccat ccagaacgcc
    45541 tatcaaggtt tcgccaagca aaattacggg atgctggaca acttgaaatt aggttttggg
    45601 ggcacgaagt ccgaaatgga acgtttgctc caagaggctg agaagatttc tggtgtcaaa
    45661 tatgatatct cgaacttcgc tgatgtgact gaagcgattc acgttattca aaccgaaatg
    45721 ggcatcactg gtacaaccgc caaggaagcg agtgagacga taagtggttc gattgacggt
    45781 atgaagtctg caatctcaaa cttgatggct ggacttggta acagcagcgc tgacattggt
    45841 atgttgattg gcaatgtggt ggaacaattt caaaacgtca tcaagaatat tctaccggtc
    45901 attcaaaata tcgtgtcggc tttaccggca gtggtggatg ccattattca agcattgggg
    45961 cagttattgc caacactctt agaagcagta acctcattat tctcacaagt gcttgaaacg
    46021 atacttggct tacttcccac acttattcca gtggtggttc aagccgtttt aacgattgtt
    46081 caaacactta ttgagaactt acctttactg attgacgctg cctttcaact tgtgacaaca
    46141 cttatccaag gactaggcga agcgctgcca gaacttattc caattacagt acaggcaatc
    46201 ataacgattg tgaacggctt gattgaaaac ttgccattac tgttggatgc tgctttacaa
    46261 cttatcatgg gacttgccca aggacttatc acagcacttc caatactgat tgaaagtctg
    46321 ccacagatta ttacaggaat cgtgaacttt attatcagtg ctattccaca aatcattcag
    46381 actggtattc aacttttgac atcacttgtg acagctttgc cggatatcat caatgcgatt
    46441 gttgcagcta ttccagtgat tattcaaaat atcatcaatg ccgtgattgg ggcaatccct
    46501 cagttgattg acgcaggtat tcagttactt gtggcattga ttggtgcgtt accgcaaatt
    46561 atcacaacga ttgcgaatgc catgccgcaa attatcaacg gaataacagg aactttggta
    46621 aacaacatcg acaaaatcat caatgctggt gtgaagttgc ttgtggcatt agttcaaaac
    46681 ttaccagcaa tcatttctgc cattgtcaaa gcagtaccgc aaatcatcac agggcttgcg
    46741 aatgctttcg gtgagtatgt ttcaactatg ggaactatcg ggaagaactt gattacagga
    46801 ttgtggaatg gtatcgctga tgctggaagt tggttacgca acaagatttc aggattcttt
    46861 ggcggtgtag tagacaacat caaggacttc tttggtattc attcaccatc aacactgttt
    46921 cgtgatgaaa tcgggaagta catggcacaa gggattggcg taggttttga taaggaaatg
    46981 actggtgttt ctaaaacgat gcagaaagct attccaacaa aactttatat gccaagcttt
    47041 gatattgata ccgatatcca cgctgccgtt gatagcacat tctcgatgct tgatttaggt
    47101 gctaaggttg atgccttaac aaacatcacg agtgaaatgt tgccaacgct acttaaagca
    47161 atggacatta aagtcgtctt gaatgatgga acattggtcg gcaaacttgc tccagaaatt
    47221 gataagagct tggcgacgtt gaatcgtaga cgtgtagcat attagtgttg gattttcaca
    47281 ttttgactgt atactataac taacgagtgt ttactgaaga gatggtggta atcatatgtt
    47341 gaagcataaa tttaaagcaa agaaacctgc actgatagga tatattctac tgcttgctat
    47401 tggattattt ttaacggcag taagatggta tagcggcata aatcatggtt ttgtattcat
    47461 caacaatcag attaattctc acatttcaaa cttcgcatta agctttcttt tctatttggt
    47521 gataggctat ctttggttgc ttatgggaac gaatttaagg gttcttactg cgatgggagc
    47581 tggtctgatt gttgcaaata ttgtatgtga aacgatgatg acttttctga atacaccaga
    47641 tctaatcgat gcaatatatg gaatttccgg cgtgctggtt gtatacgcgt atttagcgat
    47701 taccaagaaa atagggctgg aagaaacaac cgaataggat gatagattat aaactttagg
    47761 attagacatt gttgtctggt ccttttttag ttatttagat gggcggtggt caattgaacg
    47821 catttatttt agacggaaca attaattcac ggactgattt aggtttgcga attacggaag
    47881 tgccagttat tccagtgacg gaacgtgtca ttgatagtat tgagattgat ggacgtgaag
    47941 gagatttgac cattctgaaa ggctggaaag atgtgacttt cggttttaag gtagtgattt
    48001 ggaaatctga tgtttggact agctggcaga taattttgcc acaaatccta aacgctaaaa
    48061 ctatcgcatt ttcaaatgaa ccaaacgtgt tttacccaat caagtatgct aaggcgagcg
    48121 gactaactca gattttatct tccatgtggg agtttgaaat ggagttaacc tgttcgcctt
    48181 ttcgttataa gaccaatgta gcgactatca atcgaaccac gtcaggaacg gtcaataatc
    48241 ccggcaacat ttactcattg ccgaagatta aggtctacgg caatggaact aggacactca
    48301 cgataaatgg caagcctatc gtgttaaatc tgacgaatgg caacctgaca ctagattcgg
    48361 atttgaaaga atgcttttac ggggatgtgg cagccaatca atacatgaca ggtgactttc
    48421 ctgagtttcg agtcggcagt aatactgtga ctcttggtac aggtataacc aaagtagaaa
    48481 tagaaccgag gtggcgatac ttatgattac gctttacaac aaaacagaat caaatttcaa
    48541 tcataacggt ctcgctgtcc ttgatggcaa cattctgaac cccgtggtga gtgaggaatt
    48601 gaacggactc ttttcctttg aattcgatta tccaattcat gctccacaca gtgaggaatt
    48661 gttgccagaa atgattgtga aagcaccagt accagaactg tccgaccagc ttttcaggat
    48721 tgttgaacga gatgatgcac ttggtggttt tttgcacata gtagctcatc atattttcta
    48781 tgacctagcc acaaacttta ttgaagatac ttatatcgtc aacaagaacg ggtcgggagc
    48841 tttgacacaa ctgcttggtg caactcaatt catgcaccgt tttaccggta catcaaacat
    48901 cagtgcggtc aataatgtcc gcttggtgcg actaaatcct gtagaggtgt tgcttgatag
    48961 tgacctagag aatggttttc aaagcagtta tggtggtgaa attgtacgcg ataacttcac
    49021 gatttcaatg ttaacgactc gtggcagtaa caacggcgta caaatccgag acaagaagaa
    49081 tctgacagga tataaagctg atgttgatta ctctggaatt gttactcgca ttatgccaca
    49141 agggtatgat ggactatttc ttcccgaaaa atacgtggac agtccaaaca tcaaccaata
    49201 cgtccagcct aaaatcaaag tcatcaagta cgacaacgtc aaagttggtg atgaagagga
    49261 tgagtttact caagccgagg cctatgatga acttcgcaga ttggctgggt tagaatactc
    49321 agctaacaag gtcgatttgc caaaagccac ctatgatgtg gagtttgccc cgcttgagcg
    49381 aactgaagaa tataaagatt ttgcacagct tgaaacgatt aatttgggtg atactgtttc
    49441 agttattcat gccgaagatg gattgaacat cacagctaga atggtctcat acaaatacga
    49501 tccatttctg aaagcgtata tctcagtaac actcggaaac atcacgccaa aattcacgga
    49561 tgtggcgaag gacatcaacc gggtggacaa caaggcggag caagccaaag acgatgccaa
    49621 ttacgcattg agttcggcaa atggtaaaaa caccaacttc tatggcccga caactccaaa
    49681 taatccgaaa ctcggtgacg tttggtacaa agaaaatggt gacaagtttg aaatgtgggt
    49741 gtatgaaatc cgtgatggcg tcactcagtg gtatccactt atgacagata tgacggcaga
    49801 agaagtaaag caagccgtgg aacaagcaca aaaggatgcc aatactgcca ctgaaaaggc
    49861 caatcaagca tttaacgatg ctgtgaatgc tttgaatgca gctgaaagtg taggtgcaag
    49921 tgttgaaacg cttactacat ccgttgaaca gaatactagc aagatttcat tagtggcgag
    49981 tggtatgtca actcttgaag gacgagtaat cacagctgaa tcaactctga cagtgcaagc
    50041 tgggcagatt gctagtaagg caagtcaatc gacggttgat aatctgacag ggcgtattac
    50101 aactgcagaa agcaccatca cccaaaatgc tgatagattt gagctatctc tttctaaaac
    50161 aaataaagcg gtcaatgttt tagccggtat gagcattgca gatttgcagt gggcagttgg
    50221 tggtctgaat acaaatgatg gaaaagaaaa tacctatggt ggatacgttc gctcaggttt
    50281 tacaagagtt agaaatggcg aaaagtatct acttcaaaca tttgtgggtg ggaaacttta
    50341 ctctacttac gcaacggctt atctgcttta ttacaaagag gataagtcct ttttatcgta
    50401 cacccaattt ggaaacacca ccacaccgct tactgttcca agcagtgccg cttttattcg
    50461 cattcgttta acaactagtg atgcaccaga aaccatcagt tgctatatgt tgaaaacgga
    50521 tgtgacaaat ggctatgttg atttatccga gataagtaac gtcgtaaaac ttacagcgac
    50581 cactgatgcc ttactgttaa gtgtttcaga aaccaaagaa gcaatcggcg ttccatttaa
    50641 agtcaatcaa tgggaacaag gaagtttgag taccactgac ggttcagaag tgtccgcaac
    50701 caattatctc cgaagtgagt tccttgatgt caaagtggga gataagtata tcgggcaact
    50761 gcgtgacgga actcaagctg ctttctacta tcattactac ggcatggatt taccttacat
    50821 tgattttgtc ccaacggcaa aacaatttag tgaaacccta acggcgggaa cttatactaa
    50881 tgcgaccatt gcgagttatc tagtaagtaa gaacgttgaa tcgatgagta ttacaagtgg
    50941 cgtgacaaca aacccctatt caaattcagg agattatctg gtcatttaca aattgccatt
    51001 ggaaggcaaa gtcgctccga gtgtaatttc gtggaatggt cgcaaggata catcagataa
    51061 tattgtcttg ctttatacag gtggtgcttg ggaacagatt tcgaccgtta caagtagcag
    51121 caatgccact tattcttgga cgttaacggc tgagcagata tcagggatta ctactgacag
    51181 cgtgtatcta gctttctttt cagttaagac tggcacatat gctgggattt ataattcaag
    51241 caacacacct tttactctaa acccgtcgga tggttcaagt tatcatcaaa tctcttatac
    51301 tggttcgagc ggagcaatta cagtcccgag tggcacattg aaaatgcgag tccgagtgaa
    51361 cacgagcatc aaacccgatg aatatgaagg aaatatttat cctacgacca caaggcaaga
    51421 ctataccaat gccaacacat tatattcagc aattctcatg cagaaagact taatcaattt
    51481 acgagttggt aaaggagacg tgattaatca aatcaatatc tcacctgaaa gtattttgat
    51541 tgctggaaac aaggtgcaca tcactggtca aacaaccatt gatagtgcag tgattagttc
    51601 tgccatgatc caaaatgcgg ccatcacaaa cgcaaagatt gcagatgcca caattcaaag
    51661 tgcaaaaatt gcaagcattg atgcggcgaa gattaccaca ggaactttgt cggcagcccg
    51721 tattggcgcg aactcgatta cggctgataa gattgctgct aatatcctaa cagcattgac
    51781 tgcgagttcg tccattcgaa tcacaggaac tacgattgga tattattcag gaagcactct
    51841 tgtgactgaa ataaactcgc agggaatgac cattcgacgt ggtggaacga cagtcggaac
    51901 aattggtgca aataacatca gtggtcactc tgattggcgt ggattggtat ttgatcttga
    51961 gtatggaaca gagtatatgt catgggctca taaagattcc tcctctgcga gtgtctacac
    52021 ccagaagctt tcttggtatg cgagaacatt gcagtctggt atggagaagg ggttccactt
    52081 tgacgacgac atttatttac catcaatgct tcgtataggg acacaacatc aaatatctgt
    52141 tggtgggatt acatttagta gttcccttta tactaagatt ggctcaagtt ccggcagcgc
    52201 tggtattgca ttcaacggtt ctaatctgat gctcggagat gatgggaagt gggttgactt
    52261 tggagtcatc cgtgaaatct gtgccaaact tgcgggacga acaatcgctt taccaaccgg
    52321 tttcagtagc aacggtacag cgagtggttg gtacaatccg cagtcgttta attcaatgac
    52381 cacatacagt taggaggccg ccatgaacga agaaaaagaa gaagtgatag taactgaacc
    52441 tatcgaggca gatattccaa agccaatgaa agttgggacg gatatggttg atttgcttgt
    52501 gatggtcgtg gatgatcttt gtgtccagat tggtgattta aaggagaaga tagataatgc
    52561 tcaaaattag aaatgaagat ttggtggacg tgatgagttt cttaaaaggt tttgacttac
    52621 caccaaagat tagccgagtg cgaacgaagc ttgcaaaact catccaggtg aaggttgatg
    52681 aactctatca agacgaggta gacttgcttc aaaaatacgg aaagaaagat gagcacggca
    52741 atttggtcca acatgacggt gccttttcac tcatcccaga aaccgcaact gaatatcaca
    52801 aagaaaaggc agacttgctc aacgaagaaa cccacatcaa cgtggatgaa cttcaagaca
    52861 agctgccttt attggttgct ggatttgaaa atagtgaagt caccatttca gggaaagatg
    52921 ctgaaatgct cgatttaatt ttagaacaat tggaggattt gtaaatgaaa gaagtgtgga
    52981 attggattca actcgccatc gcaggtggtg gaggagtgat tggatggtat gtgggaggaa
    53041 ttgatggttt gatttatacg cttgtaactt ttgtggtagt ggactatgta actggtctgc
    53101 ttcgtgcaat caacgaaaag aaactatcca gcaagattgg ggcaaagggc atcactaaga
    53161 aaatcatgat ttttcttttg gttggtgtgg ctcatatgct tgatgtggaa gtcatcaaag
    53221 gtggaaatgt actgcgtgat gcggtcattt tcttctatat ctcaaacgaa ggaatttcac
    53281 tgctggagaa tgccgtggcg attggactgc cagtgccgga aaagttgcgt gaaatcttgc
    53341 agcagttaca cgaaaagaag gaggaaacag aaaatgggaa ttagtaatct agcaacaaaa
    53401 tatggatttc agtcatttag ccattactcg aatggtcgta gtggtgccaa agtagataag
    53461 atagtgattc atcacatggc aaccaccaac tatgatattg ttcctgatat ttggaaaacg
    53521 cgtgaggcgt cagcacacta cggtattgga aaaaatggag aaattcgagc atacgtggac
    53581 gaaggaaaca cagcttggca cgctggtaat tggaacgcca atatctcaag tatcgggatt
    53641 gagcactgca atagcactgg atcaccgaac tggggtatca atcaagcaac tattgatgca
    53701 agtgcaaagt tgtgtgcaga tattgccaaa cgttatggtc tagggaaatt ggtggtgaac
    53761 aagaatatct tccctcactc acatttttcg gccacatcat gtccgggaca gttgcttggt
    53821 aaactgcagg agattgcgga taaagccaat gcgattaata atggttcatc aaccccaagc
    53881 acgccagcta gtggtctgta tcgtgttcgc aaatcatggg ctgacgccaa gtcacaaatt
    53941 ggtgcctata aggacttgaa caatgcgaag cgggctgcag atagccattc tggttataag
    54001 gtgttcgacc agaacggtaa tcaagtttat ccatctgggt catcaacatc caacgtgaaa
    54061 actcttgtgg ttcaagtgag tggtctcaac gttcgcaaca agccaagttt aagtggtgcg
    54121 gttgttggtt catacaataa aggcgcaact tggacgcttc cgaagtcaga gaagatgacg
    54181 attgcggatg gttgggtatg ggctcgttgc ccgattggat attgtgctgt cggtaagaat
    54241 actggtaaga cggaatctga cgattatatt ttgatttcat aaatttaatc ggaacaaaat
    54301 tatgtccgga ttgttgactt aaaatatcga acgtgttatt attaattcaa ccagaacata
    54361 aatttgttcc gattgaatta tagaaagagg tatcgatatg gttacgttta aagatttgtt
    54421 accagaacca acagaaagcc aacaagcttt cattttagat cacgggaaaa cagaagatga
    54481 tggtcagttg aaatacgctg atgatgctaa aagttacggt tggaacatgc gccaatatgg
    54541 taaattgaaa gccggcgcgg ttgttttgaa tcgtcatccg gggaaaatta ctaaagaccg
    54601 taagtgggaa atttacgggg gcggatacgt tgagagcatt tctgaacctg atgaagctgg
    54661 aaatgttacg gcagtaatta cacacgcatt tacaatcgaa ccggcaatta aacaaggtga
    54721 ttcatttatt gaaaacttcg attggaatac acctagcaaa aagaagcgta aaaagccgaa
    54781 ttcatgggcc tacttctggg atcagtatgg aatgaatgaa attacctatg ccgattttgt
    54841 tgcattgatt gaaaatcgtc atcttatccc aatcgatgat acacaatcgt tgccgtctga
    54901 actggatctt acaaacgagg aagttgaaga aattgagcaa gaaagttcaa aaggatttac
    54961 tgttcttgtt gatgaagtgg gaccaaatcg cacgaatggg actcagaaac gaaaattcac
    55021 tggacgccat actgattggg agcgtgtcaa caaggcaaaa caaaaaacgg gtgctctagg
    55081 cgaagaaatc gtacttgatt tcctcattca ggaagcagaa aagaacaaaa caaaacttcc
    55141 tgaacatgtt tctaaaactg aaggagatgg tcatggctat gatatttgtg cgtttgacca
    55201 atcaggtaat gaaatccata ttgaagtgaa agcttcaaaa acaaacttct ctgacggatt
    55261 tgaaatgagt actaatgagg ttgctgcctc ccttggagat acaccatata aaatttactt
    55321 tgttcacgac cttgatgtgg cgtcaaaagt ttgcaaaatc aaaatttatg atggcccaat
    55381 tactgaagaa aacttcacga tggttccaac gagctacaaa attttcaata agtagtttga
    55441 cgtctttata attcaaaaca gcctcacaag ttttattgtg gggccttttt ttcgtctagt
    55501 tttctttaag gtaggtgttt acagcatttt gcattaggat taaatgattt cttaactgaa
    55561 atacctgctc tttgtaattg atgcagaaaa gaggcgattc tattgattcc atacgattat
    55621 tcatcgcgtt tatattggct tgtaaaaact ggtataaagc ttcttcagcc tctatggttt
    55681 taaagcactt ttcaaattct tgtttgaagc tttcatcaaa ttcctccagt tcctttgttg
    55741 tcgcagattt gcaaaaatgt tcatattctt tttctaaccc ttccatatca aaactactca
    55801 taactttctc cttcccacaa ttgatattta tattgttatt tattggatga agcttgtaaa
    55861 ggcttatttg atgtttggga agtaaaaagt caaaatttac aaagaataat tcctactata
    55921 tataaacaaa cttagaagcc tgcaagagtg attcgtttca ttcttgcagg cttttttcgt
    55981 tttgtgggtt cgagtcggtt atttctttcg catatggatg aaaggcaaag gaaaatttgt
    56041 gattgggttt actttcatgg attttcttcg cctgtatctt gaggaggcga aagcaatgac
    56101 taacaagaaa acagaaatta actattcaga tgaagcagcc tattacattt cacagcagat
    56161 ccttctgaac ttacttgtta ccggtaaaat caacctggaa gaatacgtca aaatcgacaa
    56221 taaaaaccgg gaaacattca atcctttttt atcaaggata atggtctgaa acgttgatat
    56281 aacggcactt tagagctaac atgtcaccta atggaaagga gttgagctga tgaaaacgat
    56341 acataaattg gcacgtccag agttgccgga agcttccaaa ctaaaggtgg cagcatacgc
    56401 tcgtgtatca acatcaagta atgaacagct ggcaagttta caaacccaga ttactcacta
    56461 tgaaaatcac atcaagaacc atgaacaatg ggactatgtc ggtgtttatt atgatgaagg
    56521 gatttctgga accaagactg taaaacgtga tggtttaaaa cgattgatta aggatgcaga
    56581 gcttggaaag attgatttga ttctgacaaa gtctatttcc agattttcaa gaaatacagt
    56641 tgactgcttg aatctggttc ggcagctaac tgatattggt gtcaccatct tttttgaaaa
    56701 ggaaaacatt aatactggtg atatggaaag cgagttgttg ctttcaattt tatcaagctt
    56761 ggcagaaagt gaatcctatt cccattcaga aaacttgaag tgggcgattc gaaaccggat
    56821 gtctaaaggg aaattcaaag cttacccgcc gtatggctac aagcggaaag gctatgattt
    56881 tcacatcgtt gcagctgaag caaaagttgt taaacaaatg ttccagtggg cattagatgg
    56941 catttcgact ggaatgattg ccaagcggtt aaacgatgga aaacaattca ctaaacgtgg
    57001 ggcaaattgg caagcaacat cagtcagaag tattttgaaa aatgaagttt ataccgggac
    57061 tttgacgatg caacaaacct ttgttgatga ccagtacaaa cgacgcataa acaagggcga
    57121 attgccaatg taccagatca aaaacaatca cgaacccata gtatctaaag aagattacga
    57181 ccgagttcaa gaactcattc ggagaaaagc tgaggccaaa gggaatactg gtgacgggaa
    57241 aaagtacacc aagcgatacg cattttcagg caacatcatc tgtgataaat gcggcagcaa
    57301 attcaagcgg gttcacactt attggaaagg taatacgaag aaggttcagt ggaagtgtac
    57361 ggggcatctt aaaaataaag atagctgcga tgcattacca attacggatg aagatttgaa
    57421 ggttgcttat ttgaccatgt tgaataagtt gattttcgga cacaagttgg ttctggaacc
    57481 ttttatgaag tctttgaata aagcaggtgc gaatcaggaa gggctcgaag tgcttgctga
    57541 tgaaatcgac aagattcaag aaaagcttga ggttctggca agtttgactg cttccagtat
    57601 tttatcaacg aaggtgtctt tagaagaaca aagcaaactt caacaagaga gggctgtcgc
    57661 acaagaaaag cagcgcctca tcattgagtc agtcaacggc aaaagcactc agcaaattga
    57721 actagaagcc ttatacaaat tcgcgcggat ggcagagatg ttgacggact gggatgaaga
    57781 tttattcaag aagttcacaa acagcattac agtttacaac cgccaagaag tcggatttga
    57841 acttaagtgc ggactacttt tgaaagagag gttaccagaa tgaacaatac aattccatac
    57901 gggtatgtca ttaagaatgg agaaatcaac gtggatgaac agcaagctca acaaatacgt
    57961 gagttcttca atatttacct tgagggtgtt ggcttacaag aagctggcaa acgctctggc
    58021 attaacaagc cgcatccctt tatgaaacgt attttgatga atgaggttta cttaggaaat
    58081 gagatatatc caaaaatcat tgatgaggat attttcttcc aagcggatct ggagcaagaa
    58141 cgaagaatta agttctttaa aaaacgcaag gagatcaaag tgccagatac gaaggtaata
    58201 cccactcatt ttaaatctgg caagattacc gaaaagtatt ctgacccatt caaacaagcg
    58261 caatatgcat attcaaaaat caaggaggtt aagtgaaatg gcaggaaaac aggtgacggt
    58321 tattccaatg aagcctagac aatgggcagc agagaatacc gaagagaaac caaagttgaa
    58381 agtggcagcc tattgtcgtg tttctactga aatggaggaa caggcgagtt cttacgaagc
    58441 acaggttcaa cactatacgg actacattca aagaaatccc gattgggagc ttgtaggaat
    58501 atttgcggat gaagggattt caggaactgg gacgaaaaag cgtgacggtt ttaaccggat
    58561 gattgaggcg tgtcaaaacg gcgaagttga gtacatcatc acaaaatcaa tttcacgctt
    58621 tgcccgtaat acggttgatt gcttgcaata catacgacag ttaaaagaac tgaagattgc
    58681 agtgtttttt gaaaaggaaa acatcaatac aatggatgcg aagggtgagg tacttttgac
    58741 cattatggca agtctggccc agcaagaaag ccagtcacta agtcaaaaca ccaagatggg
    58801 cgtacagtat cgtttccagc agggacagct acgtatcaac cacaatcatt ttcttgggta
    58861 cactaaggac gaagatggta acttggtgat tgaaccaaag gaagcagaag tcatcaaacg
    58921 catcttccgc gaataccttg agggcagttc cttgcaagaa attgctaatg gtctaatggc
    58981 tgatggcatc ctaacgggag ggaagcgaaa gctctggcga ggagaaggag tcagattgat
    59041 tcttcgaaac gagaagtaca tgggggatgc gctgcttcaa aaaacatata ctaccgattt
    59101 cttgacaaag aaacgagtga agaatgacgg gagttacgct cagcagtatt atgttgaaga
    59161 tagccatccg gccattatcc caaaagatat cttcatgcaa gcccagcaag aactagaccg
    59221 gcgtaaatca atgaaaaaca aaaagtcaca gtgcttttct gggaagtatg cgttatctgg
    59281 tatcacagtt tgcggtgact gtggcaatgc ttaccgcaga gttcattgga agaatcgagg
    59341 aactgtgtgg cgatgcaaat ccagagtgga taaacgagaa cataattgca cgggtcgaac
    59401 aatctacgaa acagacctgc atggagctgt tatcaaggca atcaatgaaa cgttaatcga
    59461 tcgtgatgct tttcttcagc aattaaatga caatatcaac tcagttatga ctgatggatt
    59521 aactgaccga ttggcagaac tagacagtca gttgaaagag cttgaagctg aaattattag
    59581 cctggcagtt ggcggtcagg gttacgatga attggcaagc caaatatttt cactaagaga
    59641 tgaacgagat gcggttgcaa aacaaatagc agcgaatact aatttgcaac aacgggttga
    59701 tgagatggta gtctttgtaa aagaacacga tgtcatcaat gagtataatg aagtgcttgt
    59761 tcgaaggttg attgagaagg tcaccatttt tgaaaagaac attgttgttg atttcaaaag
    59821 tggggtgaga gtgactgtag aaatttaatt tgaatagata ggactagacg ttaatgtggg
    59881 tagccatatt aacgtcttta ttgattggat ttcataaagt gattgatgat ataatatatt
    59941 atgtaaaaaa taggctgggg gcgaagttct gtgagccaat ggaataccat tgatgaatta
    60001 agggatattt ttattcgtaa atatacggat tctgagttga gtaatgaact tgaaaaggcc
    60061 tgcagcgaag aatatgaatt gatgcgtgat tataatgggc gtcagatttt agagttatta
    60121 cagaatgtag acgatgccta tggagatata aaatcagaaa atgatgctag caatgacgaa
    60181 gtgaaagttc agataacata taaaaataat atcttggaag ttgggaatac aggcacttca
    60241 ttttcaaaag atacaattga aagattgtgc ttaggaagag cgagtaataa atcatcacaa
    60301 aatatcggaa ataaggggac tggttttcgt tccttgctaa acgatgctga gtgggtcgaa
    60361 ctgtattcgg gagaatttgc tattcgattt agtgaagaat tcacaaaaaa gctttttgag
    60421 cagtatgtat caagagattc tgacaaattc agtgaattaa tttttgagca gaagaaaaat
    60481 tggaaaaaag aagattatga tttatgtttt ccaataatga actgtcctca accaataaac
    60541 aaaattgatt ctggttttga tacattaatt cgagtaaaac taaaagaaac taatttaagt
    60601 aaaggcacga gtgtttctaa acaacttcag cagccatttt ataagtcact gttattttta
    60661 ccaaatataa caaaaattgt tattgaaaca actgatgatg aaaaaatatc agagaaaatt
    60721 gtggatggaa atgatgtatt gatagaaaaa agtgatgtta ctggtcaatc accgtctgaa
    60781 gaatattttg tttttgacaa agaatctatg ataggagata aagttgctaa aattgcacta
    60841 gccgtttcta aagattctga ttacgattac tccaaagaaa aactatattg ctactttcca
    60901 gtacgtaatt ttggtacacc aattcatgca cttataaatg caccgtttat aacaaataat
    60961 tcacgtgatg acgtcccgga tgatagtgaa caaataaata agaaaatatt tattgaggtg
    61021 ctcaaattta tttctgaagt agcagaaaaa ttatctaagc ctgaatatga agacatagca
    61081 ttaaaaatgg taactccttt taaagagaat aaactatggg acttagatgt atttgactta
    61141 atgaacgaat tcttgggtat tttatctaat gcccaaatac taccgactgt caattggcaa
    61201 tatatttcta tcaaagataa tccaaaactt ttccttcaag aattccctga ggagctaata
    61261 ggtgagacat tttccacctt gttgcaatta ttagaaacaa acgaaaatga ccaactagta
    61321 caagaacttg ctaattataa tgaatacact ggcctgtcgt ttgttgtaga tgaactatct
    61381 gataaaatca acattctttc atcttcttgg gaaaatagaa taaggacaaa agtgttctta
    61441 tggtggagtg agcaatataa aagttctccg attgtcccta agctattaaa ggacaatagt
    61501 gacaattggt tgatgaaatc agataaggta ttcttaccga ccgatacagg tgttactgtg
    61561 cttccaaaag agctgtcttc ttgggttagt ttaagcattt tgaaacaaac atatgttgat
    61621 gagttaattt atcagattaa gacttcatca agttggaaaa ataaatggga agaaacagct
    61681 aatgcataca aagctgaacg tacaggagat aaacgtattc tagatgcatt cagtgaaaaa
    61741 tatcttgcaa ttgaattcac tgagcaaagc agttcagatt tgatcattgg aacgattaat
    61801 cgacaaattg acactgttga aaaatctatc agtttcatta attggtttta tgataactac
    61861 aatgaaaaac tatcagctgg aagtgagctg tctaaagttc catttaatct tccaaacgtt
    61921 gctggtgacg ttcaaccttc caataaaatg tatattggaa aagagtatgg caatgaatta
    61981 ggagataagc tttttcaaga tacagattac acggcattga aaaaaataga ttgctctagt
    62041 tttgctgagg agagagattt tctgtcattt attcttcgat gtggtgtttc caaatatcct
    62101 aagatttatg ataacgattc ttta
//
LOCUS       DASJTC010000005        60232 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.005, whole genome shotgun sequence.
ACCESSION   DASJTC010000005 DASJTC010000000
VERSION     DASJTC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.005"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(63..911)
                     /locus_tag="U4B19_000286"
     CDS             complement(63..911)
                     /locus_tag="U4B19_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2076056.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1135..3075)
                     /gene="thrS"
                     /locus_tag="U4B19_000287"
     CDS             complement(1135..3075)
                     /gene="thrS"
                     /locus_tag="U4B19_000287"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEN2076057.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3412..3591)
                     /locus_tag="U4B19_000288"
     CDS             complement(3412..3591)
                     /locus_tag="U4B19_000288"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEN2076058.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3638..5461)
                     /gene="cadA"
                     /locus_tag="U4B19_000289"
     CDS             complement(3638..5461)
                     /gene="cadA"
                     /locus_tag="U4B19_000289"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN2076059.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5739..8018
                     /locus_tag="U4B19_000290"
     CDS             5739..8018
                     /locus_tag="U4B19_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2076060.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8111..8758)
                     /locus_tag="U4B19_000291"
     CDS             complement(8111..8758)
                     /locus_tag="U4B19_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076061.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8937..9656)
                     /locus_tag="U4B19_000292"
     CDS             complement(8937..9656)
                     /locus_tag="U4B19_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2076062.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9787..10938)
                     /locus_tag="U4B19_000293"
     CDS             complement(9787..10938)
                     /locus_tag="U4B19_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEN2076063.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11155..11895)
                     /locus_tag="U4B19_000294"
     CDS             complement(11155..11895)
                     /locus_tag="U4B19_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2076064.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11899..12222)
                     /gene="rsfS"
                     /locus_tag="U4B19_000295"
     CDS             complement(11899..12222)
                     /gene="rsfS"
                     /locus_tag="U4B19_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEN2076065.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12279..12872)
                     /gene="yqeK"
                     /locus_tag="U4B19_000296"
     CDS             complement(12279..12872)
                     /gene="yqeK"
                     /locus_tag="U4B19_000296"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEN2076066.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12856..13506)
                     /locus_tag="U4B19_000297"
     CDS             complement(12856..13506)
                     /locus_tag="U4B19_000297"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEN2076067.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13516..13827)
                     /gene="yhbY"
                     /locus_tag="U4B19_000298"
     CDS             complement(13516..13827)
                     /gene="yhbY"
                     /locus_tag="U4B19_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEN2076068.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13843..14952)
                     /gene="yqeH"
                     /locus_tag="U4B19_000299"
     CDS             complement(13843..14952)
                     /gene="yqeH"
                     /locus_tag="U4B19_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEN2076069.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14949..15476)
                     /locus_tag="U4B19_000300"
     CDS             complement(14949..15476)
                     /locus_tag="U4B19_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEN2076070.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15656..16441)
                     /locus_tag="U4B19_000301"
     CDS             complement(15656..16441)
                     /locus_tag="U4B19_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEN2076071.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16434..17303)
                     /locus_tag="U4B19_000302"
     CDS             complement(16434..17303)
                     /locus_tag="U4B19_000302"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEN2076072.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17304..18683)
                     /locus_tag="U4B19_000303"
     CDS             complement(17304..18683)
                     /locus_tag="U4B19_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEN2076073.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18691..19110)
                     /gene="fabZ"
                     /locus_tag="U4B19_000304"
     CDS             complement(18691..19110)
                     /gene="fabZ"
                     /locus_tag="U4B19_000304"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN2076074.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19135..19611)
                     /gene="accB"
                     /locus_tag="U4B19_000305"
     CDS             complement(19135..19611)
                     /gene="accB"
                     /locus_tag="U4B19_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEN2076075.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19614..20849)
                     /gene="fabF"
                     /locus_tag="U4B19_000306"
     CDS             complement(19614..20849)
                     /gene="fabF"
                     /locus_tag="U4B19_000306"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEN2076076.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20868..21605)
                     /gene="fabG"
                     /locus_tag="U4B19_000307"
     CDS             complement(20868..21605)
                     /gene="fabG"
                     /locus_tag="U4B19_000307"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEN2076077.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21617..22534)
                     /gene="fabD"
                     /locus_tag="U4B19_000308"
     CDS             complement(21617..22534)
                     /gene="fabD"
                     /locus_tag="U4B19_000308"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN2076078.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22641..22865)
                     /locus_tag="U4B19_000309"
     CDS             complement(22641..22865)
                     /locus_tag="U4B19_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN2076079.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22918..23880)
                     /locus_tag="U4B19_000310"
     CDS             complement(22918..23880)
                     /locus_tag="U4B19_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEN2076080.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23890..24330)
                     /locus_tag="U4B19_000311"
     CDS             complement(23890..24330)
                     /locus_tag="U4B19_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2076081.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24636..25601
                     /locus_tag="U4B19_000312"
     CDS             24636..25601
                     /locus_tag="U4B19_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2076082.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25658..25888)
                     /locus_tag="U4B19_000313"
     CDS             complement(25658..25888)
                     /locus_tag="U4B19_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEN2076083.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25974..26861)
                     /locus_tag="U4B19_000314"
     CDS             complement(25974..26861)
                     /locus_tag="U4B19_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEN2076084.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26950..28173)
                     /locus_tag="U4B19_000315"
     CDS             complement(26950..28173)
                     /locus_tag="U4B19_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2076085.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28190..29236)
                     /locus_tag="U4B19_000316"
     CDS             complement(28190..29236)
                     /locus_tag="U4B19_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEN2076086.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29400..31127)
                     /gene="ptsP"
                     /locus_tag="U4B19_000317"
     CDS             complement(29400..31127)
                     /gene="ptsP"
                     /locus_tag="U4B19_000317"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEN2076087.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31127..31393)
                     /locus_tag="U4B19_000318"
     CDS             complement(31127..31393)
                     /locus_tag="U4B19_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEN2076088.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31872..34106
                     /locus_tag="U4B19_000319"
     CDS             31872..34106
                     /locus_tag="U4B19_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2076089.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34254..34574
                     /locus_tag="U4B19_000320"
     CDS             34254..34574
                     /locus_tag="U4B19_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN2076090.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34779..35003)
                     /locus_tag="U4B19_000321"
     CDS             complement(34779..35003)
                     /locus_tag="U4B19_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HEN2076091.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35201..36781)
                     /locus_tag="U4B19_000322"
     CDS             complement(35201..36781)
                     /locus_tag="U4B19_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEN2076092.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37182..38558)
                     /locus_tag="U4B19_000323"
     CDS             complement(37182..38558)
                     /locus_tag="U4B19_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN2076093.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38686..39804)
                     /locus_tag="U4B19_000324"
     CDS             complement(38686..39804)
                     /locus_tag="U4B19_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2076094.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39946..40380
                     /locus_tag="U4B19_000325"
     CDS             39946..40380
                     /locus_tag="U4B19_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2076095.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40458..40991)
                     /locus_tag="U4B19_000326"
     CDS             complement(40458..40991)
                     /locus_tag="U4B19_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEN2076096.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41085..42263)
                     /gene="mutY"
                     /locus_tag="U4B19_000327"
     CDS             complement(41085..42263)
                     /gene="mutY"
                     /locus_tag="U4B19_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEN2076097.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42256..43053)
                     /gene="recX"
                     /locus_tag="U4B19_000328"
     CDS             complement(42256..43053)
                     /gene="recX"
                     /locus_tag="U4B19_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEN2076098.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43160..44542
                     /gene="rlmD"
                     /locus_tag="U4B19_000329"
     CDS             43160..44542
                     /gene="rlmD"
                     /locus_tag="U4B19_000329"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEN2076099.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44716..45294)
                     /locus_tag="U4B19_000330"
     CDS             complement(44716..45294)
                     /locus_tag="U4B19_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEN2076100.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45616..46086
                     /locus_tag="U4B19_000331"
     CDS             45616..46086
                     /locus_tag="U4B19_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEN2076101.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46194..47420)
                     /locus_tag="U4B19_000332"
     CDS             complement(46194..47420)
                     /locus_tag="U4B19_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2076102.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48174..48374)
                     /locus_tag="U4B19_000333"
     CDS             complement(48174..48374)
                     /locus_tag="U4B19_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2076103.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48508..49116)
                     /locus_tag="U4B19_000334"
     CDS             complement(48508..49116)
                     /locus_tag="U4B19_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEN2076104.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49201..49671)
                     /locus_tag="U4B19_000335"
     CDS             complement(49201..49671)
                     /locus_tag="U4B19_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEN2076105.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49719..50633)
                     /locus_tag="U4B19_000336"
     CDS             complement(49719..50633)
                     /locus_tag="U4B19_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2076106.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50779..51582
                     /locus_tag="U4B19_000337"
     CDS             50779..51582
                     /locus_tag="U4B19_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEN2076107.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51760..52707
                     /locus_tag="U4B19_000338"
     CDS             51760..52707
                     /locus_tag="U4B19_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEN2076108.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(52775..53068)
                     /locus_tag="U4B19_000339"
     CDS             complement(52775..53068)
                     /locus_tag="U4B19_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEN2076109.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(53096..53761)
                     /locus_tag="U4B19_000340"
     CDS             complement(53096..53761)
                     /locus_tag="U4B19_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEN2076110.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(53901..54533)
                     /locus_tag="U4B19_000341"
     CDS             complement(53901..54533)
                     /locus_tag="U4B19_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2076111.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54540..55607)
                     /locus_tag="U4B19_000342"
     CDS             complement(54540..55607)
                     /locus_tag="U4B19_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2076112.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(55604..56335)
                     /locus_tag="U4B19_000343"
     CDS             complement(55604..56335)
                     /locus_tag="U4B19_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEN2076113.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(56504..56983)
                     /gene="greA"
                     /locus_tag="U4B19_000344"
     CDS             complement(56504..56983)
                     /gene="greA"
                     /locus_tag="U4B19_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEN2076114.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57119..58294)
                     /gene="mltG"
                     /locus_tag="U4B19_000345"
                     /pseudo
     CDS             complement(57119..58294)
                     /gene="mltG"
                     /locus_tag="U4B19_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
     gene            58498..59667
                     /locus_tag="U4B19_000346"
     CDS             58498..59667
                     /locus_tag="U4B19_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN2076115.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            59992..>60232
                     /locus_tag="U4B19_000347"
     CDS             59992..>60232
                     /locus_tag="U4B19_000347"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076116.1"
                     /translation="MIIEINNEILEKNVYEYLIVTLFIPLSLFTVTKGPPCFSTVAFF
                     ELTVTVNPLYRNTGKTIVLPFEDLNVNVISSLFGEK"
BASE COUNT        17491 a        12624 c        10157 g        19960 t
ORIGIN      
        1 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
       61 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
      121 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
      181 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
      241 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
      301 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
      361 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
      421 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
      481 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
      541 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
      601 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
      661 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
      721 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
      781 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
      841 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
      901 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
      961 acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata
     1021 tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt
     1081 atctattgtt cggattgctt tctatctact acttttttac aggaaattta cctattagtt
     1141 ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc
     1201 tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt
     1261 gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc
     1321 cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt
     1381 gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat
     1441 aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg
     1501 ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc
     1561 ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga
     1621 taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa
     1681 ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag
     1741 cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg
     1801 atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac
     1861 catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg
     1921 accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc
     1981 atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat aagaatggat
     2041 cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc
     2101 gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga
     2161 tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact
     2221 aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag
     2281 ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc
     2341 tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc
     2401 tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc
     2461 acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg
     2521 aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc
     2581 ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa
     2641 tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat
     2701 catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt
     2761 atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag
     2821 gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc
     2881 ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc
     2941 aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct
     3001 ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa
     3061 agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc
     3121 tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg
     3181 ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct
     3241 tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc
     3301 ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt
     3361 atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt
     3421 ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg
     3481 ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc
     3541 taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc
     3601 tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta
     3661 ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg
     3721 tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa
     3781 ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca
     3841 tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga
     3901 ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat
     3961 ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt
     4021 gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta
     4081 cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga
     4141 tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg
     4201 ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga
     4261 ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta
     4321 cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc agttgactaa
     4381 caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag
     4441 attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg
     4501 gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa
     4561 tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt
     4621 tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag
     4681 gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa
     4741 taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga
     4801 caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc
     4861 tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg
     4921 acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt
     4981 catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct
     5041 tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt
     5101 ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg
     5161 ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca
     5221 aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat
     5281 ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga
     5341 gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt
     5401 cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca
     5461 ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc
     5521 tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa
     5581 caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa
     5641 ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact
     5701 tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac
     5761 aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa
     5821 aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga
     5881 ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc
     5941 aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt
     6001 ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag
     6061 aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg
     6121 actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg
     6181 aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag
     6241 aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag
     6301 agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag
     6361 cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg
     6421 gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca
     6481 agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca
     6541 tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt
     6601 atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt
     6661 ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc
     6721 agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa
     6781 taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg
     6841 aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag
     6901 gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc
     6961 tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat
     7021 tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga
     7081 tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc
     7141 cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa
     7201 ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt
     7261 tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg
     7321 atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag
     7381 caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg
     7441 gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct
     7501 atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag
     7561 tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata
     7621 tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc
     7681 gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag
     7741 acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca
     7801 ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg
     7861 atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta
     7921 cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca
     7981 atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat
     8041 taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat
     8101 ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa
     8161 gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc
     8221 tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt
     8281 ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac
     8341 gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc
     8401 tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag
     8461 cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca
     8521 gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta
     8581 aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga
     8641 atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta
     8701 ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat
     8761 tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc
     8821 ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta
     8881 gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag
     8941 tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca
     9001 atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc
     9061 atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata
     9121 ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg
     9181 cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca
     9241 aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc
     9301 ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc
     9361 aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag
     9421 tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt
     9481 acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca
     9541 ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta
     9601 ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt
     9661 tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt
     9721 gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc
     9781 aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa
     9841 ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat
     9901 tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa
     9961 tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt
    10021 cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac
    10081 ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc
    10141 tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg
    10201 ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc
    10261 ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat
    10321 ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt
    10381 acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact
    10441 taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg
    10501 aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc
    10561 ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga
    10621 agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac
    10681 tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac
    10741 taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg
    10801 ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat
    10861 ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat
    10921 aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga
    10981 caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa
    11041 cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat
    11101 ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc
    11161 tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc
    11221 agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa
    11281 tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg
    11341 ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact
    11401 atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc
    11461 atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg
    11521 atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc
    11581 agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc
    11641 cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc
    11701 ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc
    11761 tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga
    11821 aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa
    11881 tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga
    11941 ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac
    12001 atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc
    12061 atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc
    12121 gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa
    12181 agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc
    12241 tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa
    12301 ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat
    12361 gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca
    12421 tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg
    12481 tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct
    12541 gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt
    12601 gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac
    12661 tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca
    12721 ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc
    12781 ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa
    12841 gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa tataatcaat
    12901 tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt
    12961 cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt
    13021 tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt
    13081 ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc
    13141 cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc
    13201 taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt
    13261 gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg
    13321 catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg
    13381 tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa
    13441 gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt
    13501 catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt
    13561 tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat
    13621 ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga
    13681 tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt
    13741 gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa
    13801 acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg
    13861 tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt
    13921 gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg
    13981 aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac
    14041 atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt
    14101 tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc
    14161 gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa
    14221 cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat
    14281 aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa
    14341 ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat
    14401 gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg
    14461 tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc
    14521 actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca
    14581 ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa
    14641 aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc
    14701 aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg
    14761 caaaaagtcg tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa
    14821 acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt
    14881 gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa
    14941 ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag
    15001 gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata
    15061 atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata
    15121 atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg
    15181 ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca
    15241 atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca
    15301 tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca
    15361 atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca
    15421 ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg
    15481 tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact
    15541 gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt
    15601 acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat
    15661 attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt
    15721 ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact
    15781 gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg
    15841 gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa
    15901 tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt
    15961 catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga
    16021 tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc
    16081 cgcgctcttc tgctcctaca ccgcaataag cacctgctgt attgatcaag gtaatcaccg
    16141 gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat
    16201 ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga
    16261 tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg
    16321 caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca
    16381 agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca
    16441 cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat
    16501 tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg
    16561 aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg
    16621 atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact
    16681 cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt
    16741 ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt
    16801 gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat
    16861 aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc
    16921 atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca
    16981 tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc
    17041 ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa
    17101 gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct
    17161 tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta
    17221 tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat
    17281 ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc
    17341 ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga
    17401 ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt
    17461 aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt
    17521 gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact
    17581 cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca
    17641 aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact
    17701 acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact
    17761 aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt
    17821 tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca
    17881 taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt
    17941 gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa
    18001 tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata
    18061 tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct
    18121 gcttcttgct gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga
    18181 atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct
    18241 gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg
    18301 acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt
    18361 gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta
    18421 ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg
    18481 ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt
    18541 gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg
    18601 gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta
    18661 gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc
    18721 ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac
    18781 tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt
    18841 tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa
    18901 tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc
    18961 ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat
    19021 ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg
    19081 aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt
    19141 ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat
    19201 ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat
    19261 aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc
    19321 gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa
    19381 tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac
    19441 attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact
    19501 tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag
    19561 cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc
    19621 tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt
    19681 gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga
    19741 tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt
    19801 gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg
    19861 ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc
    19921 tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt
    19981 acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta
    20041 ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt
    20101 ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca
    20161 cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt
    20221 gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct
    20281 tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc
    20341 ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca
    20401 cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt
    20461 ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga
    20521 agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta
    20581 tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata
    20641 tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca
    20701 cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc
    20761 gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact
    20821 ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg
    20881 accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa
    20941 actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc
    21001 gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc
    21061 gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat
    21121 cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca
    21181 gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg
    21241 tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct
    21301 gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg
    21361 atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag
    21421 atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct
    21481 tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag
    21541 gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca
    21601 tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa
    21661 cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg
    21721 ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga
    21781 aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta
    21841 tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta
    21901 ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga
    21961 catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta
    22021 ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata
    22081 cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca
    22141 tcactgcaac cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt
    22201 ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat
    22261 attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc
    22321 gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac
    22381 gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat
    22441 caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg
    22501 caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat
    22561 atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc
    22621 ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga
    22681 accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg
    22741 atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat
    22801 ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat
    22861 accataataa tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta
    22921 caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt
    22981 ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata agatagggat
    23041 gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat
    23101 ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata
    23161 atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa
    23221 atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata
    23281 gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc
    23341 tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc
    23401 tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt
    23461 ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct
    23521 taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc
    23581 tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt
    23641 tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc
    23701 cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat
    23761 acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt
    23821 agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat
    23881 taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa
    23941 ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa
    24001 atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg
    24061 gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc
    24121 agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt
    24181 cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg
    24241 agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata
    24301 atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat
    24361 caaatctttt gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc
    24421 atttttttac tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca
    24481 gcatttataa tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt
    24541 attttctgtg aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt
    24601 atagtctttt tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc
    24661 tccccatgct gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg
    24721 gtaaagcaaa acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt
    24781 caaaaaaagt attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca
    24841 cggaattaga tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata
    24901 accgacaaat gaattatttc agtaattata attatgtaca tattaccaat aagggatttg
    24961 tattaacaga tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc
    25021 cgtctatttt gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt
    25081 tgttagcttt gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa
    25141 aggaactcca agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa
    25201 gacaacaaca tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata
    25261 tcgaattagt tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac
    25321 catcgctcac cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg
    25381 tttgttatgg tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct
    25441 attatgaaaa gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg
    25501 atcctgtaca aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg
    25561 aactgttaaa caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt
    25621 aggcatcttc aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt
    25681 ccatcgctac gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta
    25741 attcaaatgt atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt
    25801 caaaacggcg aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta
    25861 tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat
    25921 tgtatcaaaa gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat
    25981 tttttttctg gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca
    26041 ttctcaaaac ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc
    26101 aatacaagat ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt
    26161 ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa
    26221 gaccagttag ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg
    26281 tttttttgtg catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc
    26341 tgattagcca tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta
    26401 ccaaattctt tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg
    26461 atcgtcagtg taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca
    26521 tctgcgcctt tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta
    26581 gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag
    26641 tagatttctt ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct
    26701 gctaccgctt ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt
    26761 gcttttgtcc gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca
    26821 gcacttccca tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa
    26881 tcaaaaatcc ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat
    26941 gtttgaattt tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc
    27001 ttcttctttt cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat
    27061 catagcttcg ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg
    27121 atctttagga acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt
    27181 ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc
    27241 cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga
    27301 agtagaagca ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc
    27361 cacttctcct gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa
    27421 ctcttctaca taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat
    27481 gacctgagga aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa
    27541 agaaagaatc atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc
    27601 ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg
    27661 aagcggtgct attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac
    27721 tacacctgtc gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat
    27781 cacttttcct ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat
    27841 cagaggtatt ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt
    27901 atgggtatga ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc
    27961 tctaacaaca actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac
    28021 atctttttcc aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc
    28081 atgtctttct aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg
    28141 aaagtaggta tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga
    28201 cctgatttac tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat
    28261 ttttaagttc ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt
    28321 gtgtcgtact cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt
    28381 ttgtcttccc catatagcag ttcttgctat cttctaacca gccattatac actggaatat
    28441 ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct
    28501 cttcataaga agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc
    28561 cacgaatgta accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga
    28621 tgttccgagg aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca
    28681 tcttcttagc aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac
    28741 aaatgattac tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca
    28801 ttggatcagg gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac
    28861 ggataccata gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg
    28921 ctttttggta caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatctga
    28981 taaaggaatt tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt
    29041 ctcttctggc tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga
    29101 tgtcataatc ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt
    29161 tttgatgatc aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat
    29221 ataataatac cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac
    29281 tgtcactcaa acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa
    29341 aaagaacaaa aaaactggcg tagctctttt acaagccaca ccagcttttg atacgttgtt
    29401 tattttacat attcatgaac aagttcaacg acttcttcca tcatatcgca ttctttcaat
    29461 gcacgatcag caagttcagc cattttcgct gtgtccaagc gtttcattaa actacgcgtt
    29521 ttaagaatag atgtcgcact cattgagaac tcatctagac ccatacctac tagcaatgga
    29581 acagctgttt gatctccagc catttctcca cacatacctg cccattttcc ttcagcgtgt
    29641 gctgcatcaa ttacattttt gatcaaacgt aaaattgatg ggttatatgg ttggtacaag
    29701 taagaaacgc gttcgttcat acggtctgct gccattgtgt attgaatcaa atcgttcgta
    29761 cctacagaga agaaatctac ttcttttgca aacttgtctg ctaagactgc tgctgctgga
    29821 atctcgatca tgatgccgac ttggatcgta tcagatactt ccttgccttc acttatcaat
    29881 ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga attctttgag tgtagctacc
    29941 atagggaaca tgatacgtaa attaccatga acagatgcac gaagcaatgc acgcatttgt
    30001 gtacggaaca tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg atatcctaag
    30061 aatggattca tttcatgagg taattgcaga tatggtagtt ctttatctcc accgatgtcc
    30121 attgtacgaa caactacagg ttttccttcc ataccttcta atacagcttt gtaagcttca
    30181 tattggtcat cttctgttgg gaagtctgga gaatccatat acaggaattc agtacggtac
    30241 agaccaactg cttctccacc attattatgg acaccaacta gatcttttgg tgttccgatg
    30301 tttgcagcta attcaaaatg cttgccgtca gctgtgacag tttctgcatt ttttaatttt
    30361 tcccattctg ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc agctttttgt
    30421 tcatctgtag gatcgatgat tacatcgcct tcgatcccgt tgacagccaa aatatcgcct
    30481 tctttgactt tagcagtaat ttcttttgtc ccaacgattg ctggaatttc taaagaacgt
    30541 gccatgatag cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa
    30601 ttgcgatcta attgtgctgt atcacttgga gtcaagtcat gtgcaacaac gaccacttct
    30661 tcgttgatca ttgatggatt tggtaaagta acgcctaata agtgcgctaa aatacgttta
    30721 gcaacgtcac gaatatctgc agcacgctct tgcatataag cattatcttc cattgcttcg
    30781 aacataccga tatacatatc cgttacttct tttagcgctg attcagcatt tacgctgttg
    30841 tctttaatat tttgcttgat ttgacctacc atttctggat ctgaaagaac catcaaatgg
    30901 gcatcaaata cttgcgcttc tgcttcacct aagctttgcg ctgctttttc acgaatttgc
    30961 tgcaattctt cagtagattt agctaatgca gcatccaaac gagcttcttc agccgcagta
    31021 tcttctactg ttgccttact gaatgttaaa tccggttgaa ctagcaggta agcttttgca
    31081 acggctacac cgtcactagc ggcaatccct tttagcattt caaccattat tcagataagc
    31141 cttctttctt cattgtttca acgattgctg ccattgcatc agcttcgtca gcaccttctg
    31201 cagtaatagt aacatcagaa ccttgaccta cacctaaaga cataacgccc atgattgatt
    31261 tcaagtttac tgatttacct ttatactcta agtttacatc agagttaaat ttgcttgctg
    31321 tttgtactaa tagtgtagct ggacgagcgt ggatcccagt ttctgctact acgtgaaatt
    31381 ctttcttttc catattaatc ggtctccttt aagtgagtgt tgtttttatg tgagggtttt
    31441 cttttttagt aattcagaga atcctaacaa atgccaccct ttcatctgta aagattaaca
    31501 ttttttttcg ttagatacaa cagttttcat tcaatttcag ataaaagtat tcaaattatt
    31561 ccggattctt tttcatcctt ttcttcacat tttataacaa acgtgtttat ttcctttttc
    31621 ttgtcgcttt ctcttctcct gacgtttatc attctttcct ttgaccctct ttgttttcat
    31681 atgaaacgaa ttacttgctt ccctttttat ttttagtata ataagagtca aaggtcagga
    31741 atggtcaata gagatttcac cttttctacc tcataaatct cttaaaaaac taggggttat
    31801 tcttttacct catgaaaaac gagatgtaaa atatggatgt acccattttt gtgaaaggac
    31861 gtgaatgatt catgctttgt caaaactgcg gcaagaatga ggcaacgatt cacttgtatg
    31921 ccactgtcaa tggtcagcgg acacaactcg attactgtca gagctgttat caaaaaatca
    31981 aaaatcaaca aaatggaggt ctgactaata tggcccaaaa tgatccattt ggatttggca
    32041 gtttggacga tcttttccgt tccatgtctc gccaaatgca acagcaagga aattatgagc
    32101 aaacaccacc aacacaattt ggcggaaaca acaacttcaa tggtggtcag ccacctcaag
    32161 gcaatacaga aggtttattg ggagaatacg gaatcaatat cacagaacaa gcacgacaag
    32221 gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa
    32281 atcgccgaac caagaacaat cctgtcttga ttggagaacc aggtgttggt aaaacagcag
    32341 ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca
    32401 aagaagtcat tcgtttagat gtcgtatctt tagtccaagg tactggtata cgaggacaat
    32461 ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca agcagaaaat gtgatcctat
    32521 tcatagatga agttcacgaa atcgtcggtg ctggttctgc tggtgatgga aatatggatg
    32581 ctggtaatat tttaaaacca gctttagctc gaggagaatt gcaaatggtt ggtgctacta
    32641 ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg
    32701 tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac
    32761 gttatgaaga ctatcatcat gtaaaatata ctgatgaggc catcgaagca gccgcaactt
    32821 tatccaatcg ttacgtccaa gatcgcttct tgccggataa agccatcgat ttgctagatg
    32881 aaacaggttc taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa
    32941 aactgcaaga agcagaagaa caaaaagttt tagcttctcg agaagaagat ttcgaaaaag
    33001 cagcttatta tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt cagctcactg
    33061 aagaggaaac acctgtgatt accgaaaaag atatggaaaa aatcgttgaa caacggacag
    33121 gtattccagt gggtgaatta aaagaaaaag aacagacaca actgaaaaac ttagcagatg
    33181 acttgaaagc tcatgtcatc ggacaagata acgctgtaga tcgagtagcg aaagcgattc
    33241 gccgaaaccg tgttggatta aacaaacaga atcgtccaat cggctccttc ttatttgttg
    33301 gaccaactgg tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa ctattcggtt
    33361 cacaagattc aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta
    33421 aattgattgg ttctcctcca ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa
    33481 aagtacggag aaatccttac agtcttgtct tgttagatga agtagaaaaa gctcatccag
    33541 atgttttgca tatgttcttg caaatcttag atgatggtcg attgacagat gcacaaggaa
    33601 gaacggtcag ctttaaagat acgatcatca ttatgacaag taatgccgga actggtaaag
    33661 tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc
    33721 aattaaacaa ctactttaca ccagaatttc tgaaccgttt tgacgggatc atcgaattca
    33781 gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact aatgctagac gatgtcaacc
    33841 aaatgctggc agcacagcaa cttcacatcg aagttcctac gaatgtcaaa gaaaaattag
    33901 ttgacttagg ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg atccaagaac
    33961 aaatcgaaga tggtatcgct gaattttatc ttgatcaccc ttctatccat gagttgaaag
    34021 caaaactgga taaagatgga aaaatcatcg ttacttctaa accagaacgt ttggctaaag
    34081 aatcagccga agaaacggct gaataaatcc agttttatag cgtgagacgg tatttctttg
    34141 agaaatgccg tctcattctt ttttcagaaa agtcaaaata ctgttcattt tataaaaaag
    34201 aaattataat aagagaaaca agcaattaaa aatacataaa agaggtgagc gccatgaaat
    34261 tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa aacactgatg
    34321 cccatctagt caaggtctac acagccggaa ataccactat tgtctacact gaagcccctg
    34381 aacataatga aattctgctg atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag
    34441 atgctaaaaa ttatttctta gataaaatca aagacgccgt ttaccacgag gatgaaatca
    34501 aagtaatcga attggcaggc cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta
    34561 cattagccgt gtaaacaaat tttgaacata ataattaaag agcgtgggac aaaagtaaag
    34621 aacacttttg tctcacgctc taaacacgta taaacggcga aagcagaagc aactccttcg
    34681 gaaataagct gaaattcacg aaaattgctt ttcaaatttt tgtgaattcc agcttatttc
    34741 ttggagttaa acgcttctgt ctcagcctct ttttctttct agctttttga tttttctctt
    34801 ttcttttgta cttttctctt ttcattttca gttctacgcg tgtctttatt tttaactttt
    34861 tcctctttat ggatgattga tcgtactttt tgtgcaattt gctcgttttt gtcccagtct
    34921 tttacatcta ataatcgaca taatcgtaac atctcttctt ttttctgttt ttttctttca
    34981 cgtctttgcc attctgtttc catgtgtcac actctccttt ctttaatcat atgtacgcaa
    35041 aataattttt atattttttt gattagacat ttctatgatt ttaatatagt aaaaagagcc
    35101 gtcacggaaa atccgtcacg gctctaaaca aaatatgaga ggctgttttt gtttggctat
    35161 tcttattact tgatgcaggg cagcttgtgt caagacttct ttacatcaaa ctttttaatt
    35221 ttacattggg atatttatcc gcaaaccagc gttctgcaaa ttggttctcg aataagaaca
    35281 acggttggtc gaaacggtca cgagccaaga tattgcgact tgaactcatc ttctcatcca
    35341 aatcttctgg atcgatccag cgagcgattt tgtgtcccat tggagacatg attacttcgg
    35401 cgttatactc atttaacata cgatattgga atacttcgaa ttgtagctga ccaacagctc
    35461 caatgatata ttcttccgtt agatatgttt tataaagctg aatcgctcct tcttgaacta
    35521 attgatagat tcctttgtgg aaggattttt gtttcatgac attttttgca gtcactttca
    35581 taaacagctc tggagtaaat gaagggagtt cttcataagc aacttttagt tttccttcga
    35641 acaatgtatc cccaatctga taattgcctg tatcatatac accgatgata tcgcctgcta
    35701 ctgctttttc gacgttttct ctcgaatctg ccataaattg tgtgacattg cttaatttca
    35761 gttttttatt tgtacgttcc aaccagacat ccattcctcg ttcgaatgtt ccagaacaga
    35821 ttcggacaaa agcgattcgg tcacgatggg ctggattcat atttgcttgg atcttgaata
    35881 cgaaacctga aaattcttcc tcataaggac ttaccatctg atcatcttgt gttttgtgtc
    35941 cataaggagc tggtgccaat tcaacgaaag tctctaaaaa agtctgtaca ccgaagttag
    36001 tcaatgcaga accaaaaaat actggtgtct gatcgccacg agcaattttt tcttgattaa
    36061 atgcatctcc agcctctttg attagttcta cttcttctaa gacttgttca tataaggaat
    36121 tttcagttag aggatgattt tcgggaagca gtccatcttc atccaattct actaatcgtt
    36181 catcctgata attttccgga cgataaaact ctacacgatt atggtagata tcataaagac
    36241 cttctagccc tttacccata ccgattggcc aattcatcgg ataagattcg atatctaata
    36301 gctcttctaa ttcttctaac aattccaatg gttcacgacc atcacgatct agtttgttga
    36361 taaaagtaaa tatcgggatg ccacgttttt ttacaacttg gaataatttc tttgtctgtg
    36421 cttcgatccc ttttgcacta tcgattacca tgaccgcact atccacggcc atcaaagtac
    36481 gataagtatc ttcagaaaaa tcttcatgtc ctggtgtatc caagatattt actcgtttcc
    36541 cttgataatc aaactgcata acagaactag taacagaaat cccacgttgt ttctcgattt
    36601 ccatccagtc tgatttggca aaattacctg tttttttgcc ttttacggtc ccggcttgac
    36661 ggatagcccc accaaacaat agtaattgtt ctgtaatcgt tgttttccca gcatccggat
    36721 gggaaatgat cgcaaaagtt ctgcgattgt ccacttgttg ctttaatttt ggattattca
    36781 taacctaact ccatttcttc cattcaatta tttatatttt ctctaggcac agattgtccg
    36841 atctcttaaa gaaagacaat ttcatatagg gctgtgacaa aagccgtgta gcagtctctt
    36901 tttccgagta aacggcgttc tagagacagc ctatcagcaa taagcccacc caagccaaaa
    36961 atacgaaaag ctgtttttgc ttggctgctc ttattactta atgcaggata gctttttcac
    37021 aacctttgtc aactactaca actgaatcag tatagcatgg attcagcgaa tgaaaaagcc
    37081 accaaaccat aagagtcaaa tatttttttg tcagctgact aaaagaaaag acgtgttgct
    37141 tgtttattca agcaacacgt ctttcataac caagtttcca attatcttct tggctcgtca
    37201 tcttcgaatt cttttctgaa gtatcttcgt tcttcttcag gttccgcttc tgtttcttcc
    37261 ggatcatgaa aaatgacacg gattttaagg attcgagaac cttccatttc ttcagaaacc
    37321 agcgtaatat tatcgacatc aaacgataac ttttcgcctt catccggaat catgcctaat
    37381 gctgtaatca aataaccagc catcgtatct acatcactca tatgcagatc actgtcaaaa
    37441 gcttcattga actcatcaat cagcatacgt ccttggatga tatattcgtg ttcatcgatt
    37501 ttttcataaa gattctcaac ttcatctgat tcatcatcga tttctccgac gatttcttct
    37561 aaaaggtctt ctagtgttgc taacccaacg acgccgccat attcatctaa taaaatagcc
    37621 atctgattct gcgtcttttt catttcatat aacagatcat caataaagac cgtttcagga
    37681 acgaataaag gctcctgcat gattttcttg atatctaaat tatcaaagcc gaatttatgg
    37741 gcagctttta gtaaattctt tgtatgaagg attccgacta ctttatcttt gtcttcgtta
    37801 taaacaggga ttcttgaata gttttcggat aaaacttcat tcacattttc ttccacagtg
    37861 tcgttgatat caatcataaa tgcatccgtt cgtgggacca tgacttctcg ggcaactttc
    37921 gtatcaagag aaaaaacccc ttggagcatt tctaattctt cattattcaa tacgccttcg
    37981 ttttcaagca tataacgcat ttcatctctg gtcatttttg aatcttcatc atcaaaagtc
    38041 atcggcgtga tacgcgccaa taaacttgtg gaagcagaaa gcagccagac gaagggctta
    38101 gctactacac caatcaagcg tatcagccct gaagtgaact ttgcgacttc ttctgattta
    38161 tttaaagcaa tacgtttagg gtacaattct ccaaaaacaa tcgatacata ggtcaaaata
    38221 gccagcacaa tgatatttgc aatatttttt gccgctgtgc cgccacctaa taccggagct
    38281 aaccgttccg ataaagtatt agctagtgaa gcaccagaca aaatgttgac caaagtgatt
    38341 cctacttgga ttgtcgatag gaaattattt ggattctgca atatttttaa aagcttttga
    38401 gatttcacat ctccttcatc agctttttgc tcgattcgat tcttattgat cgaaacaaca
    38461 gcaatttcgg aagctgctaa aaaagcattg atcaatgtca aaacgatcaa taataaaatc
    38521 tgcgctaaca gcgactgact ctcagggtca gcattcataa tcgcattctc tccttataaa
    38581 attaattttt agtttacgat agaaaaaaac gtggcactat agccacgtta aaatggtatc
    38641 acaaagcaat tttctttgca ataaaaacaa gtgtactaaa actacctacg aggtcgtagc
    38701 aattttcttc ttgttttcat tttgcttgtc ttctttgacg attttgatta tataggaaat
    38761 gatcgtcttg cagattgcgt aaaaaggaac acctaagaag ataccaagca atccggcaat
    38821 atttccagct actagaagaa cgataatgat cgttaatgga tggattttca gtgatttccc
    38881 aattacatta ggataaatga tattcccatc caactgttgg acgattagca caaccacaca
    38941 acaaagtaat gcttttaccg gatcattgaa gactgttact agaaatgcag gtgctagtcc
    39001 tagataaggt cctaaatatg gaattaggtt tgtcaatccc gcaatgaccc caaataagaa
    39061 agcataatcc acaccaatta ccatatatcc taaaaatgtg aaggtaccaa cgaataaaca
    39121 ttcgattgct tgtccgctaa tgtaattagc caatgtttta ttcagttgcc ccaataactc
    39181 cacaatttgg tttcttcttt tttctgggaa aaactgttgg atgtttggca ctagtttttc
    39241 tccgtctttc aacatataga acaagatgaa tggtgctgta atgattacta cggttgccga
    39301 agcgatcgtt ccaacgatcg aacctaaact attggatact ccactaagga attgctgaat
    39361 gattgtcccg taagaaatgt ctaattgctc aaaatattta ttgatatcta cttccttgaa
    39421 caaaggcaat tcggctacct cttttaacca gctttctacc tgtttgatga aatcaggcat
    39481 attagatgcc aatgaggaaa tttgctgtac tagacttggg atgacactaa gtaaaatcca
    39541 aataattgct gcaactaaaa ggagcagcac aattgcgaca gcccatattc ttttgatttt
    39601 tgctttcatc aacagcttga caataggatt caataaatag taaagaaagc ctgcaatcag
    39661 tacaggtgca aataacgtgg aaaaaaatgt cccgatcggt tcgaaaataa agtttatttc
    39721 tgatgaaatc atgatcaatg cagctatgat caataattca agcgaccaaa acattagttt
    39781 agactgtcta agtttttcaa acaatagatt tcctcctttc tttcttacta gtatatatta
    39841 tattcgttaa aaattctttt attctttttt tatttttgaa aacttgccat actttttatt
    39901 tgttttattt tccagtatac tatacgaaag gaaggtggct agaccatgaa agttgtacaa
    39961 acaaaagata caatgagcaa tatttactta gatgctgtac ggatcaggca tcaagttttt
    40021 gtagtcgaac agggtgtgcc tttgtctcga gaaatagaca aagatgaagc ccattgtatc
    40081 cacttcgtcc tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct tttgccttta
    40141 gaaaacggaa aaatgaaatt acagcggatg gcgattcttt ctgaatacag acaccaaggg
    40201 ttaggaaaaa tattgattga agaagcagaa aactttgcta agaatcaagg ctataatacg
    40261 attttattag gggcacagtc cacagctgaa actttttacg aaaaactagg atatacagct
    40321 tacggcgatc cttttgaaga tgctggcatg cctcatattt atatgaaaaa gactttataa
    40381 aaatttattt cgaaaaaagc aaaaaacagc aagcttttga gaaaaatctc tcactggctt
    40441 actgttcttt tttgtgttta cttacgcgac aattgcttgt aacggttata ccaaatatca
    40501 atatagcctt ccgagaacgg gccatctcca ttattgatcc agtcaaccag aatcttcaca
    40561 ttttctttta ggatgaagtc aatatcgttc gggtaattca tcattttgct gtgcatctca
    40621 tattcatcga catccaatag tcgtttctcg ccgtcaggaa ataccttgat atccaagtca
    40681 tagtcgatat atttcaaggc ctcatcgtcc agcaaaaaag gtgaagctag attacaataa
    40741 taggaaaccc ccttttctct gatcattgct attacattga accagtattt tttatgaaaa
    40801 taaacaatcg ctggttcgcg cgttacccat cggcgtccat cagattcagt gactaatgtg
    40861 tgatcattca cgccgatcat agagtgttcg cttgttttta ataccatggt atctcgccac
    40921 gttcgatgca atttgccgtc gtgcttgtaa ctttggatcg tcacaaactc tccttctttt
    40981 ggaactccca tagcaaccct tccttaagca gcattttgtg ctttgtgttt gggtgttcat
    41041 tacaccgaca tcataattat tatatcaata aaaatgagct ttgcctagaa aggattccta
    41101 ttgtttcgat ttttctctaa ctgatctacc agtttcattt ggaccttagg aaaaacgacc
    41161 gaatcaaatg catcaggctt caaccacttc gaagttttgt tctcggtaaa ttcttgttct
    41221 gctccctcgg ttgcccgtcc gtaaaaaagg agaacatgcc atttcagatg actaaaaata
    41281 tgagtaactt ctcctaaatg tcgtgtctgc caaacaaaga gttcctgctt ttcaaatggg
    41341 agattttgct cttcttctgc tactaggtca tcaaacagat cgatttctgg ctttgtttcc
    41401 cattcttttt tcaatcgctc atactcctct tgtgtcactt ccatcattgg aaatgtccac
    41461 atattcgcca gcaatttttg gctatctctt tcttcaaaat aatacgctcc agaatgattt
    41521 tgaagagctg cactgatata ataaacgtct tttggctttg ctttttttgt ttttacagga
    41581 aaactcgttt ggattcctcg tttgttagct aaacaaaagg cttggatcgg acaagtctca
    41641 cactttggag aagttggtgt acagatagct gaacctagat ccatcattgc ttggttgaat
    41701 tcacctggat gcttttcatc aatgattttc cgcatcgctt catcaaagat tttcctgcta
    41761 gaagcttttg cgatgtctgc ttcaatgcaa aacaatctgc tgactactcg catcacgttg
    41821 ccgtcaactg caggttctgg aagtccaaat gcgatacttg caatcgcgcc tgtcgtatat
    41881 ggtcctattc ccttcaatga gctaatttct tcaggcgttt gcggcatttc cccatcaaat
    41941 tctgacatga tctgtttagc agcagcttgg atgttccggg ctcttgagta ataaccaagg
    42001 ccctcccatg cttttaaaag cttttcttct ggagcatttg ctagctcttc aattgtagga
    42061 aaccattcca tgaaacgata aaaataatcg atcactgtgt ctactctggt ttgctgaagc
    42121 atgatctcag agatccagat acgataagga tcacgattat agcgccaagg caggtttctt
    42181 ttttcttgtt cataccattg aatgaactga tcttgaaatt cttttgtttc tttatctgtc
    42241 catttttccc attctttatt cactttcctc ttccttttcc gtaatgaatc gctggatcat
    42301 atccaaatca aatccttttt gatataagct ttgctttact tttagatttc tttttttagg
    42361 ttcaaacctt aaatttcttc gccatagttt atctccttgt ttgactaaat aatcatattc
    42421 tatttcttga tctgcttctt gcggaagatc agctatcgct tgttggatca cttcttgaga
    42481 aaagcctttc gtcatcaatg tttgttggat ttttctcaat aattcttttt ggctttttcc
    42541 gtgatttttt ccaaacgctt tttcggctgt ctgatgtgca ttttgaactt ggtcttcaaa
    42601 tgtatattgg ctgagcgctt gttcaatgat ttcaggtttc aaaccttttt gctgcatctg
    42661 ctgcgctaat ttctttggtc ctttgtcact taagcgcata ttcgtgcgca cataactttc
    42721 cccatagacc aaatcatcga caaggtccaa ttcttttaag cgggctacga ttttatttcg
    42781 atcttcttga aggatttcct tttcttttaa gtatgttcgg atttcttttt ccgatcgcaa
    42841 ctgataactt aagtaattca ttgctaggtg aaaaccgaaa tcagctccgc tactcttctt
    42901 gatctcttgg atagtttctt cgtctaattc ccgtcctttt aataatcgat agcgtactaa
    42961 tacatcttca gatacttgta aagcttctcc ttcagataaa tctacgcgat agaaagggcc
    43021 tttgccttta ctgattctcg taacggttaa cataaacatc ctcctcgaac atatattctc
    43081 tatcatacca cagaaaatcc ccgtaatcag gtattatatg ttagaataaa ccacagaaaa
    43141 aagaaaaagg gagatactta tggcagaaac aattgtcaaa actggacaaa cggttccttt
    43201 gaaaatcaag cgtttaggca taaatggtga aggaatcggt tatttcaaac ggttgatcat
    43261 atttgttcca tatgcactac caaaagaaga agtactagtg aaaatcacga aagcaactcc
    43321 tcgctatgca gaaggagaat taataaaagt caaaaaaaca agcaaagatc gcgtagtggc
    43381 accatgtcct gtttactatg aatgcggtgg ctgtcaatta caacacttag cttatcaagc
    43441 tcaattggat tttaaaaagg atctgctttt gcaagcattg gaaaaattca aaccagcagg
    43501 ctttcgatct tatcaacttt tgccgacgat cgggatggat gagccttggc attatagaaa
    43561 caaagcacaa tttcagttac gaaaaaataa gcagactcaa aaaattgaag ctggtttata
    43621 cgaagcaaac tctcatcagt tagtccctat aacagactgc ttggttcaag aacctcgaac
    43681 acaaaaagta atgaatactg ttgttcaact attgaataaa tttgatgcac ccatctatga
    43741 tgaacggaca aacagtggaa tcttccgcac aatcatggtt agaatcggtg ttaaaactgg
    43801 ggagttacaa gttgttttta ttactcgcag taaaaaattc ccgcaaaaaa acgcaatgat
    43861 ccgtgagatc aatgaacgac tgcccgaagt tgtttcaatc atgcaaaatg tccaggcaca
    43921 aaaaacgtct ctcgtgatgg gggatgaaac gatccactta tggggaaaag aagccattga
    43981 agaacaaatc aatgaagtga ctttcgatct ttctcctcgt gccttctttc aactcaatcc
    44041 tcagcagacc gaattgcttt atggcgaagg aatcaaagca ttagatgtcc agcctaatga
    44101 aacgatcgtg gatgcttatt gcggagttgg gacgatcggc ttaagtgtag caaaacaagc
    44161 caaagaagta cggggaatgg ataccgttcc tcaagcaatc gctgatgcaa aaaaaaatgc
    44221 ggaaagatta ggatatcaca acactcgata tgaagtcggg acagcagagc aagttctgcc
    44281 taaatggttg aatgaaggtt ttcagcctga tggtatcatc gtagatccac cacgtacggg
    44341 tttggataac gcattgatcc aatcattatt gaaataccct ccaaaaaaac ttgtttatat
    44401 ctcctgtaat gtttctacac ttgccagaga tctggttaat ttaagtaaaa agttcaagat
    44461 tgaatattta cagtccgtcg acatgtttcc tcagactgca cgttgtgaag ttgtcgtgaa
    44521 gatgacgaga ctcgctgact aaatgaaaga ggttgtgaaa aagtctgccc tgtatcaagt
    44581 aataagaaca gccaaacaaa aatagctctt catgttttat gaatactgta cttgtgaccg
    44641 aagagttaac aggaggacac tatttattta aaaaaagggt tgtgacatag tttttgtcac
    44701 agcccttttt gattattatt ggatattcac gtctatcact tgataaaatg catttgtcgt
    44761 atcgctgata ttccatactg ctaaaatcac attatatcct tttcgatcct gcggaagtgt
    44821 gatcgtttgg ttgactgttt ttggtggaac agttgctttg tcgtctattt ttgtgatcaa
    44881 ttcaaaatca ctaaatttca aaggttgatt aggattccaa gttggtttgg taatgtagta
    44941 atcccaagta cttgtccgat gttgtgctgt taaattccaa gtaatattta atgttcctgg
    45001 attaaccatg tttttatgcc atctgttaga tgtctgttca tctaaaggct caaagccgct
    45061 gactcctgca ctagcaattt ttccatcaat gaatgtattt ttggtagctt cgatactttg
    45121 cggttcccat tgtgctcttc cgacattttg atttaagttt cctgctgctg atgttcctag
    45181 gtatgcacgg cttcctggag aagtaatata accatgcgca aaacctggaa tgctaaaacc
    45241 gaacaaccct aaactaacta acccaataaa caataacgta ctaatctttt tcatgtagaa
    45301 acctccttca tttgataacc acattgtaaa cgctttattg tattaacaca aacattatgc
    45361 ttttataatt ttatgttgaa gtaaaaaagg tgaaaaaagt aagatgcaga ttataacaga
    45421 agtagacgag aaaagtacaa atagaaaaca tgagaatcaa atcatatgat ctttttcaaa
    45481 aacatcattc atataaacaa atgttcaact ttttaataac aacaattatc aatttttttc
    45541 ttacttcaca aactaaatac aggtcttgct gtgagccttc atggtaaact aatattatag
    45601 aaaggagtta gtaaaatgaa gatgataaaa gatattcgtt tttgggtttg tgttatagga
    45661 attgtcatat taggcttttt atcagggttg cttagcggaa atcccggaaa atattactat
    45721 tctttgcaac ttccaccatt tgcaccacct agctggatat ttgggccgat gtggacctta
    45781 ctgtatatat tgatgggtat ttctctttat ttgttactta atcacaacaa caaaaaacag
    45841 cgaaacaatc ttgtcggtct ttttgtgatt cagttcattt ttaattttat atggtctgct
    45901 ctatttttta atttaagaaa tattttcatt gcagcaatcg atattacact tttagttatt
    45961 ttcttgtcag tgcttatgta tcagttgtgg ctgcatcatc gcctggcaat gtggctcatg
    46021 attccttatt atttatgggt actattcgca actctattga attattcaat ttatttttta
    46081 aactaagcgc taaaaagaga cgtcctgtaa tttaacacat agaaaaagtg ccacctaagt
    46141 tagatttttt gtctaattta ggtggcactc tttattaacc aaactatttt tttctagtac
    46201 ccacttgttg ttccatactc attattctgg tcataaccat aaccattttg ttcctgtcca
    46261 gcgtaaggat cataactttg agtatcttga cttggtgtat agtactgact tgtatcttct
    46321 gtttgctgat aataggtatt cggatcttgg tactgatcat atgtgttttc tgaataggaa
    46381 gaataatcgg tgtacccttc actattcgat ccatcaccgt ttgtcgaatc gccatcttcc
    46441 gaatcatcaa agaacatcat tgaagcattc tcatttttca aattaggtaa tgtatcgctt
    46501 gtttttatat ttaattgttt cttcagttca ttttggatcg aaagaagatc attttttcct
    46561 aaaatttgat agtagatatc attgatcatt tgtccttcgc cttgcaactg gtcttgtttg
    46621 attgtttcaa acgccggggt gtagttcgtc gctacatcaa gcatgtcatc ccaatctaag
    46681 tccgtcgtca cattgttctc aactgctttt aaaatttttc tataattgct tacgccatct
    46741 aaacttaaaa ctttattgac gattttcgtc actacttccc gctgacgacg ttgtcgaccg
    46801 atatcgcctt ctggatcttc gtgtcgcatt cgagaatagg ctaaaccttt ttctccattc
    46861 aatgtttgtt cacctactgg tacatggaca ccatccaaag taaagtcaat tttattattc
    46921 actttgatgc caccgaccgc attgatcaaa tcttccatgc catccaaatt gatcgttaca
    46981 taatgatcaa taggaatatc gagcagttgc tcaattgaat ccatggccat ttcgacacca
    47041 ccaaatgcgt aggcatgatt taatttatct accgttccat aacctacgat attcgtataa
    47101 atatctcggt ccaaactgat gatcgttgat tttttttgtt tcggattaac tgttactacc
    47161 atcatcgtat cagaacgtcc ttgttccgtt cttcctaatc caccggtatc taaacctaat
    47221 agcaaaaccg aaaaaggttc tttgtcatca atgcttactc tctcagaacg tttggaggat
    47281 attcgattag agtttttaga aattttgttt actgtttggc tcgcttctcc cataaatctg
    47341 attccgtatg ctgataatcc ggcaatcagc aaaacgaata cccctaacaa ggttaataga
    47401 actttttgcc atcgcttcat aatacacctc tgtttcacat tttctaaaat attgtctcat
    47461 ataactgttc aatcagcaaa ttcacttttt aatcataaga aaactttaaa aaacattttt
    47521 gtgtaacgat tattattttt taaaatttct caaaattctt aactatcata ccataccgtg
    47581 aaaaagatcg aaagaccgaa tacacgcagt tcgcaatcgt tttatctttt tttatgtatt
    47641 ttttccagaa aaagcgaaca acaaggtatt ttccaaataa tcgtgcagta aaatgatcgc
    47701 acataccttt cccatctttt taacagtctt tgcctaccgt tcagattatc attcgatttc
    47761 acgacaattt cttcctatga ctggtccttt tgttaaaccc atcttctatt cgttttattt
    47821 ccagctcact gcatggctct taaaatgact cgaactagtg attgcattac catcatcttt
    47881 taatggatag atatgctcct cttcaaagta aaacgatttt cttttaaagt aatagaattg
    47941 acatggtaat gagtatgtat actagtggtt tcacaaatgg tcttttattc aacatccaaa
    48001 aactgaaatt tctttaaaat ccattactaa cgaagaaaat gttccttgct tgataattag
    48061 ttaggacatg tcaagtgaac aaatgcattt acttagccaa aaaatctaaa aacttcataa
    48121 atagacaaaa agacacgact ctctaagaag tcgtgtcttt attcaacaaa ttattataat
    48181 ttagtcactt cagcagcttg aggtccgcgt gcaccttcaa caatcgtgaa ttctacttct
    48241 tgaccttctt ctaggctctt gaagccgtct ccttgaattg ctgagaaatg aacaaatacg
    48301 tcatctccgc cgtctactgt aataaaccca aatccttttt cattgttgaa ccatttaacc
    48361 gttccgtgtt ccataaatgt atatcctcca aaaaagttct tgcaagtaac cttgcaagaa
    48421 cattataccc aatttagaaa acgctagcaa gctttcgctc ttttattttc caaattattt
    48481 ttaagactga tatgacggag gaaatggtta ttttgctgct tcgaaacgtt tgtttacttc
    48541 atcccaatta ataacattcc agaatgctgc aatataatct ggtcggacat ttttatattt
    48601 caaataataa gcatgttccc aaacgtccaa acctagaatt ggtgtctttc cttccatcaa
    48661 tggagaatct tgatttgcag tagaggtaat agctaatttt ccattttcca ttacaagcca
    48721 agcccatcca gaaccaaatc gtccagcggc tgcttttttg aattcttctt taaaagaaga
    48781 aaaatcacca aaagcttcat taatcgcttc ttttatttct cctgtaggtt cgccaccagc
    48841 atttggtgcc atgatttccc agaaaaatga atggttagca tgtccgccac cattgttacg
    48901 tacagctgtc ttgatatctg ttggaatagc gtccatatca gataataatt cttctattgt
    48961 tttttcgcct aattctgggt atttctcaat tgctgaattt aaattcgtca cataagtatt
    49021 gtgatgctta tcatgatgca gatgcatcgt ttcttcgtca atataaggtt ccaatgcgtc
    49081 atatgcataa ggtagatctg gtaatgtgta agtcataaaa aaattcctcc tcaatgtttt
    49141 cttaataatg tctacattac aaagagtaac aaagagaagg aaattcttca attgttatgc
    49201 ctagtcgtct ttttcacttt gacctacttc ttcttcaaaa acctcagttt ctttaaaaat
    49261 aaacaacttg ctgaaaatat agttcgcaag gataacaata atttgagtga tgattttcgc
    49321 cacatactca ttgctgttca taacattgat caacaatacc attgccccca tgtccaatcc
    49381 gtaagaaagg atacggtaga agaagaactt accgcattca ataagtgcat gtgtaaaatt
    49441 cggtgatttt gaatggaata cccaaagttt gttcgttaca aaagcaaaca atacagaaca
    49501 aaaccacgaa atagaattgg ataaggccat acttaaatca aaatattggc gtgcgagagc
    49561 aaaactaaca aaatttacaa tcgtagccaa accaccaaaa aagagataga ctagtatctc
    49621 ccaatatcct tttgcttcta aatatccttt taactgtcga tactgcttca tgagccaacc
    49681 taccttatta aaaattctta tttatcatac tctatatttc aataactttc atctttattt
    49741 aaaaaaatta attttttttg atcctttgcc tttgtctctg cttggatcgt cacatgtgta
    49801 ataccatatt ttaattttaa taaagaaatt atacgaggat atatcttttc aatctcgctt
    49861 agtagctgat cttcaaaatc aatatgtccg tcgaaaataa ttatcttttc gctatactgc
    49921 caaatatgga catgatgaac atcctttacc ccatcgattg cttcaatttc ttgttccatt
    49981 ttttcatagt cgatagcagg tgcactttgt atcaaaatag cggcagcttt tttgatcacc
    50041 attatcgctt ctatcaatat atatatcgaa atgatcagtg taataactgg atcgatccag
    50101 tagatatcga aaaaacggat caaaattgct ccgataatca cacttaccga agacagtgta
    50161 tcacttaaca aatgcaaata actagatttt atattcaaac tttcatctgc ttcttgtcgt
    50221 aataataata ctgagaacaa attcgcgagc aatccaataa ctgctactgt tagcatcaaa
    50281 tgactattta tcttttctgg tgaagtgaac cgcttgaatg cttcaacggt taaaaacaag
    50341 gataatatga tcaatatgaa tgagttgatg aaagcactca atatttctaa acgctggtag
    50401 ccatacgtac gttttttatt tgcttctttt cgggccattt tttgtgcccc gtaagaaaaa
    50461 ataattgcca atgtatcact aaggttatgg aaagcatctg aaacaagaga caaactccct
    50521 gacataatcc ctccaataaa ttctgataaa gtgatcaccg cattcaaaac cactactcca
    50581 aatagttttt ttgatgatct ctgtggctgt gtaggcacta ttccttcctc cattttattt
    50641 atattggata gtctttagac aagaataaca tttttttgat atttcaccaa tcattcagct
    50701 gtttccttaa agcacttgta tttcacttga tacttcgcta gaattgcaag tgatgacact
    50761 actagatgag gagtacttat gacaacaaaa caattaatta tcagttcctt tatacgattc
    50821 aaagacctaa aggaggcgag aaatgtccca ttttggaaag ccattgtcta tcttttagtt
    50881 ttgagtataa ttatggcttt gccaatctct tttcaagttt ttcaagtctt aaacaacatt
    50941 caattggatg ggcaaaagat tgctactcgc attcctgatt ttacaatcca aaacgggcag
    51001 cttcaaaccg aagaaaaaga aggattcatt taccaaacaa actccattat cttcacattt
    51061 gatccagagg gaaaacgaac agaacaggac atctctactg atttgatggg gaattttgtt
    51121 agtgtcggga tgttaaaaaa caaacttatc atagctcttc ctaatacagg cacgacttct
    51181 gctttgttga acaataacca attagaactt ccctacacca atgaatcttt aaaaaactta
    51241 acaggtaagc agctccgttc ttttttaagt gaagcatcta taccactttg gataaaagtc
    51301 ctgacattta tattttctat ttatcctagt tttctgaatc tgctgatcac tttgctattt
    51361 accaacattg ctgcttttct ctatgcacgc tttcgactaa caaaagctac ttttcttgat
    51421 tgtttaaaaa cattgatcta ttcaatttca ttgcctacta taattgcaac aatcttgatg
    51481 gtctttcttc cttcttttga cactagcgcc ttcattgctt ttgcaggaat ctttattttt
    51541 gctcaagctg tcaaaggatg ggcaaaaata tcgatcaatt aagaaataaa gacaactcgt
    51601 tatttctact tcaatagacc cttttagagg ttgtgacatt tttttgtcac agcccctttt
    51661 ttatttgaaa aatatttctt tctcaaaaaa caagcaaggc gcttcaaagt tcttcttgta
    51721 tgcgctacac ttagagtaaa gaaaggcggg aaattaacta tgtcagaaag aatcatttat
    51781 gcagatcgtc catttcgtga tgaatttatc gaagaaatcc atactatagc gcctggttat
    51841 atctttaaaa cagaattaca accagaggat ctacaaaatg tagagatcag tttgggctgg
    51901 aactcagact accaagaaaa actattagcc tcttctacct tgaaatgggt ccaatctatc
    51961 tcagctggtg ttgatcagct accccttgag actttttcaa agaaacatat tttactttca
    52021 aatggtagtg gcatccacag tgaatcgatt acggagcatg tcttaggaat catccttggg
    52081 tattctcgtg gtctttttca agcacaaaag gcacagagtg acatgaaatg gttaggttca
    52141 tcggtccatt accaatctat ccaagggaaa aaaatactaa tcattggaac tggccatatt
    52201 ggaaaaatga ttgcaaaaaa atgcaaagca ttagacatga actgctatgg aatcaataca
    52261 tccggtcatc cagcagaagg aatggaaaag actttttctt taaatcaact aaaggaagtt
    52321 cttcctgaag tagatttcgt agtcaatatc ttacctctaa cggaggaaac aactgggctc
    52381 tttgatcagc atatgtttga acactttgat ccaaaatctg tattcatcaa tgttggccgt
    52441 ggtgcttctg ttaagacaca agatctggtg caggctttaa ataaccagca actttccttt
    52501 gcagcattag acgtttttga ggaagagcct cttccacaag atcatcctct ttggaaaatg
    52561 gacaatgtac tgattacttc ccacattgca ggtttgacac ctgattttca aaagaaatta
    52621 atggctattt ttttaaccaa tctgaaaagc tactttgcca cccatgagct tcaaaccaat
    52681 caagtaaaat tgtctgcagg atattaatag ctgaaaacaa tattttcaaa agaaaaaaga
    52741 gaaggtgaca aaaattgtgt caccctctct ttttttattg ttttttaaag tgataggttg
    52801 gctcctccag gttgggatta taatcaacaa gtaaatcata tcctttaaag aaccattcat
    52861 ctgcttcctc gataaaaaaa agtgtcccat caatttccac tttctttaca ggatgttctg
    52921 gctgttcaac tgacattcca atcgaaaaac catcatgaac ctctgttttg ccgtagactt
    52981 ttccaaaaaa acgtattccg taagtttctg gcagatcaat ttctgagtca aaccactttt
    53041 gtgcttttgg cgttaccgtt aatttcatcc caacaaccta ctttctcttt tctttctatt
    53101 gattcatctt atcgtctaga atatccacat cgcctgtctc tccaataacc agtagattat
    53161 cattttcacg aacatattca tctgctgcag gagaagcgat aaccgtttga tctactcttc
    53221 gtatggcgac tactgttaag tcaaaacgtt gcctgaaatt taattcagct aatgttttgt
    53281 tgaaaaattt aggattggag actcggattt ctgctaaaga atatttatct gacaactcta
    53341 ggtaatctaa aatattcttt gacactaaat tatgcgcaat ccttattccc atgtctcgct
    53401 cgggatggac gacgcgatca gctccgatct tttcaagtac tctggcatga tactcatttt
    53461 gggcttttgc taaaatattt ggtacgccca tttcctttac catcagagta actaagatac
    53521 tggcttgaat atcttctcca atagcgacaa ttacatgatc gaaatttctt atgcctaatg
    53581 atcgaagtgt catttcatcc tgtgcatttg ccacaacagc atgagtggct atattcatat
    53641 attcattgac ccgatcttca ttactatcaa tagccagtac ttcttgacca gcttcgatca
    53701 gtgttcggca gatacttccg ccaaatcgtc caagacctac aattgcaaaa ttttgtttca
    53761 ccgttttcct cctttagtat cttcttttct cagtataaca aagcctttac aaaaacaaaa
    53821 gcaagcatca ttattgtaaa aaagccaaga aaaataatag ttattttctc agcttttgat
    53881 tatcctcttt atcatcgttt ttatttcacc aaaccatgtt tgaatgcata gatagcggct
    53941 tgtgtacggt cttccacttc tagcttagcc aatatattgg aaacatgtgt ttttactgtt
    54001 ttcagtgtaa taaacaattc gtccgctatt tcctgattac tttttccttc agaaatcagc
    54061 attaaaattt cattttcccg attcgttagt tcttcatgta atacaggctc atttcgccga
    54121 ctcatttttt ccatcatttt tgttgtcact tctggttcta atactcgctc gccacgttgc
    54181 gttgccctta tagcatcagc aatttcatgt gcggtagagg tcttcaacaa ataccctgct
    54241 gcacctgctt caatggcagg ataaactttt tcgtcatcaa taaaactggt tacgataatg
    54301 attttagctt ctggccaatc ttttaagatt gcttttgttg attcgatacc atccatttct
    54361 tccatgacta agtccattaa aatgacgtcc ggtcgaagtg tcatcgcctt ctcatagcct
    54421 tgtcgtccat tttcagcttc tccaattact tcaatgtctt cttgaataga taaataaaaa
    54481 gagacgccta agcgcaccat ttcatggtca tctactaata aaacttttat catccttatc
    54541 tatgcctcct tcatcaaagg gaccttgatt tctacacttg taccttgacc tttaaaacta
    54601 atgattttta ctgttccacc aataccagct actcgttctt tgatattgtt caatccataa
    54661 cttcccgttt tcgtttcatt catattaaac cctgttccgt cgtcaataat acgtaacaaa
    54721 agattattgt ctattttgtg caaatatacc tctaattcat tggctttagc atgtcttaat
    54781 gtatttgata acaattcttg aacgattcgg aacaagtgat cctcaatgga actagatagt
    54841 tttacatctt ctacatccca tttcagcgaa atctggattt tattttgcaa ttccttcaat
    54901 aactgctcga taccttgctt taaacttttc tcttctaaat tgactggacg tagatgaaga
    54961 agcagcgcac gcatctcaga ctgggaagcg ttgatgatct ctgctaccat tttcaactgc
    55021 ttgcgaaaca tttctggtgt ctcgcttttt tctgcttgct ccgtcaatgc cgacatcatc
    55081 atcattgctg caaacaattg ttgcgaaact gaatcatgca gctcacgggc taatcgatgt
    55141 ctttctaatt ctaaaatttc ttctttggtt tgtccatcta catattgggg acggctcgtt
    55201 acaatttgta attcactaga tacttcaatc attttttctt ttatcttagt gatttcctta
    55261 tcaatatctt tgatgtataa atcgtccgat gcacttggga tcggaagaag caacaatttg
    55321 gattcataat tgccagaaga aagtgcacgc agcttttctt cgattttccc gtattttgtc
    55381 ttttgaataa gtgataataa tagaaagaca attaatccta cacctaagga aatggctaac
    55441 aaatggaaga taagcggaac atataataaa cgtgcacgca gtaattctcc ccaccaatga
    55501 ctttgattgc tggcataaaa ataagtgaaa agtgaaaata gaataataag gaaagccgca
    55561 atccccgaat aaacagctag catcgctctg gatatttttc ccatcatatt cgtatcacct
    55621 cgacatctcc aatcaaggta ttagtaataa tcttcaatct acgagggttt tcatcataat
    55681 cactgctgta gattttgatt tgttcatttt tcaaagaaaa ttgctcttct tcaaacaaga
    55741 catttcctac taatgtcgca tgctctaatc ggatggctac gcccaaaggt actaagatcc
    55801 gtgttctgcc tatccctttt cttacaatca caatattatc atcttttggc aaaagcgtat
    55861 tgcccaaatc tatgatagta tcaccagaaa tgacggcgat gttgatatcg tcccattcat
    55921 agacgtcgtt tccaatacgt tggttaccga ataattgctg tcttttccgc tcattattat
    55981 gtgtttttaa ttgctctgtt tggatcatca tgatctgttt ctttcgccaa aaagcatttt
    56041 tggttaaatc tatccctgaa atctctacac ctttcagacc aataaataaa acggcaaata
    56101 cagccatcat ccataaagca ggactgttga ctaagctaaa aaaaatgacc aaacttccta
    56161 atatcaattg aaaattttga aatttagtac gaggatgttt gcgcatagct agatatatat
    56221 tgaaaatacc gaatatcact aaaagcaaaa gtccagtatt gtttacgatt tgccaaacag
    56281 caaataacag cagtagtgct tcaactacca caaaaaaacg ccaagaacta ttcatcgctc
    56341 tttctccctc ctgacacatt ctcatttcat catacctatt tttttaactg ttggctatag
    56401 gactttagat gtaagatcgt ttgttgaaat ctaaaataca caaagagaaa aagatctaga
    56461 gtgtatcatc agtaagatga actctctaga tctttcagtc agactacgct tgttctactt
    56521 tgacgatttt aactaacata tcgccacctg gagtagcaat agttacttca tcatttttac
    56581 gtttgccaat caatgcttgc gcaataggag aatcattgga aattttgcca gaaaacggat
    56641 ctgcttcagc gcttccgaca atcgtgtact cttcttcttc tccatctggt agttcgatga
    56701 atgtaactgt tttaccaatt gatacctcat ctgcatctac accgtcgtta tcgatgattt
    56761 gagcaaaacg aatcatattt tcaattgttg tgattcttcc ttcaacaaat gcttgttcat
    56821 ctttcgctga ttcatattca gagttttcgg aaagatctcc gaaaccgcgg gcaattttga
    56881 ttcgttcaat gatttctccg cgtttgactg tttttagttc ttctaattct ttttctaatt
    56941 tttctttacc ttctagagtc attggataaa ctttctctac catgtgtaac gtctcctttt
    57001 ttcgataaaa taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa agattaccac
    57061 gcttttcttc aaatgtaaat gtttttctaa aaatgatcaa tgtctagagc ctgtttttct
    57121 aagaattatt tacatatttt tccactaatt ccatatgttc ttcataagtt ttagcgaaat
    57181 aaacatttcc agtgctcgta tctgctacga aataataata gtcattttct gctggttcta
    57241 atactgccta gattgtctct tcgcttggat tgtcaaaagg tccaggtcca tagccggtat
    57301 tggtatacaa attatatggt gaatctacag ctgtatcttc atacgtcact aattctttgt
    57361 gttcacctaa tgcgtataaa acagagatat ctgactgaag tggcatattt gctttgattc
    57421 ggttaaagaa gacttgtgca atgtttttac gatcattttc ttttacgcct tctttttcaa
    57481 ctagagaagc tagagttaaa acttcttgta cagtcaaatt tttctgcttg atagctgaat
    57541 aatagttttg catgaccgta ttcgtcttgt tcaccatttc aatcactaga tccttaagcg
    57601 aagtactcgt gtaataatca tatgtggctg ggaataagta accttccaaa cggtattttg
    57661 tgttctcagc tttagatgca ctttccaaca gttctgggaa tttttggtgc aactcgttaa
    57721 agaaagtctc atcattcact aaatctaaaa attctttctt gtctttacca gttaccttcg
    57781 ctacccgttc agcaatctga tcgatatcgt acccttctgg aatggcaatt ttcccatctg
    57841 caacttttgt tggctcagaa gtaccgcctt cttgcaactg tttgccaatc tcgtcaagag
    57901 tcatattagg cgctaactga taatagccag cttgaaagcc agttaaattt ttaaattttg
    57961 tataataatt gaaaacaatc ccgcttttaa taatattgtc cttctccaaa atctcaccaa
    58021 tctgcttgtt agaagagcca ctcgggatct cgacttggac taattggtca tctgatttat
    58081 ctaaaggctt caacccgcta tctacataac gatacacggt aaatccaaga aatccgccaa
    58141 taatcaataa tgtaagagca atcactaaaa tgatcttcct cacaatctta tcttcttttt
    58201 tacgcagatt tctccgcttg tcgattatat ctgctgtagc tgccttactg gcttcttttt
    58261 tatctttact tgggtgttgt ttgtttcctg acaaaacaac tcctccttac tatcaagttc
    58321 gtaactttat tcattataca tgatatttga attggcgtga atgattttat ttaaatattc
    58381 tatcaaaaat gtaagtagta aatccattac tataagatta aaataaatgt tatcaagata
    58441 caagctattt tagctttatt ctttctgtaa aaaagaaagg agactaaata aactattatg
    58501 aaaaaaggaa aacatgctaa aaaaagaaaa tattcatttt taatgatcgt tgtcgtgttg
    58561 tttagcctta gtgcgttctc tacaggaact attcttggat ggcaatttta caataatgaa
    58621 cactctcaga tagttgccca tgctcaatca gctcaaaaaa agttgatact gactaccaat
    58681 gaacaacagc tcgttgaaaa aatcgatcaa ctattaagaa aaaatgatta tattggctca
    58741 atttacgtaa agaaaaataa tcgcatcatc atggaaaaag gctatggcta tgcgaataaa
    58801 ctcacgcaac aaccaaatga tccttctctt tattatcaga ttggttcggt tcaaaaagca
    58861 ttgactactc ttttgatttt aaaacaagtc gagcgtgggc acctttcttt agatactaag
    58921 ctggctcaat tctatccaca aattgctggc agccagcaaa ttactatccg tgacttgctg
    58981 tatatgcgct caggcttgca ccgaactgct tctccaacca taccgatgac tgatgaagaa
    59041 gtcgttcaat ttgcgattca gcacttaaaa tttacagatt atcagacctc tcattatgat
    59101 tcattgaact ttaccatatt gactggaatt ttgatcaaat taacaaatca accgtatgaa
    59161 aaattattga ccgatgatat catcgaacct ttaggattac agcatacaaa attttatgac
    59221 caagtcaaaa attcatccaa tcatgcacta tcctatcaaa tgtctaaaga ggatgattac
    59281 tttaaaccac tgacagaatc tgaaacagag atccataacg aattagggac tggtaatatc
    59341 agtatgtccg tctatgattt aaatacgctg tttacacaag ttttatctgg aaaactagtt
    59401 tccaaagact tactcttctc attatggcgt gaaaatgcga atggacatcc atatagtggt
    59461 ggggtatata gcggagataa ttatatcctg tcgcaaggaa atatcgactc ttttgataca
    59521 tctgctatat ttagcaaaga ttcacaggat gctatcatca tggaaagcaa tataaaagct
    59581 gacaaaacta tcaaactgcc tactgctgat ctaagaaacc aaatctatga cctgatggaa
    59641 ggaactacta agttggcaaa tagctgatat gatgaaaaga tagcaaatac tacttctttg
    59701 aatagcattt gcttaaaaaa agtactcgac taaacgtagg aaagagtgtg ggacaaatct
    59761 aaaaatcaat ttttgctaca gactttaatt ccaaataaac agaacagaaa gaggtgttct
    59821 tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacattat ttgacaaagg
    59881 gcctattaaa tctaaaatag tatctttaga ttgctaaaat atgtgctagc aaatcattca
    59941 tggcttgttc ttatttttaa ttttatgcaa taaaaaagaa gaacaattag cgtgattata
    60001 gaaataaata atgagatttt agaaaaaaat gtgtatgaat acctgattgt tactttattt
    60061 attccacttt cactttttac tgtaacaaaa ggacctcctt gttttagtac tgtagcattt
    60121 tttgaattaa cagtaaccgt taatccttta taccggaata caggtaaaac tattgttttg
    60181 ccttttgaag atttaaacgt aaatgtaatt tcatctttat ttggagaaaa gc
//
LOCUS       DASJTC010000006        58684 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.006, whole genome shotgun sequence.
ACCESSION   DASJTC010000006 DASJTC010000000
VERSION     DASJTC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58684
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.006"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(87..827)
                     /locus_tag="U4B19_000348"
     CDS             complement(87..827)
                     /locus_tag="U4B19_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076117.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(957..1904)
                     /locus_tag="U4B19_000349"
     CDS             complement(957..1904)
                     /locus_tag="U4B19_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2076118.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(2033..2797)
                     /locus_tag="U4B19_000350"
     CDS             complement(2033..2797)
                     /locus_tag="U4B19_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2076119.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            complement(2801..3190)
                     /locus_tag="U4B19_000351"
     CDS             complement(2801..3190)
                     /locus_tag="U4B19_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076120.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            3325..3867
                     /locus_tag="U4B19_000352"
     CDS             3325..3867
                     /locus_tag="U4B19_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEN2076121.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            4015..4647
                     /locus_tag="U4B19_000353"
     CDS             4015..4647
                     /locus_tag="U4B19_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076122.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(4781..4906)
                     /locus_tag="U4B19_000354"
     CDS             complement(4781..4906)
                     /locus_tag="U4B19_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2076123.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(5040..5933)
                     /locus_tag="U4B19_000355"
     CDS             complement(5040..5933)
                     /locus_tag="U4B19_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEN2076124.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(6115..7041)
                     /locus_tag="U4B19_000356"
     CDS             complement(6115..7041)
                     /locus_tag="U4B19_000356"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEN2076125.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(7126..7887)
                     /gene="pflA"
                     /locus_tag="U4B19_000357"
     CDS             complement(7126..7887)
                     /gene="pflA"
                     /locus_tag="U4B19_000357"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEN2076126.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(8131..10362)
                     /gene="pflB"
                     /locus_tag="U4B19_000358"
     CDS             complement(8131..10362)
                     /gene="pflB"
                     /locus_tag="U4B19_000358"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEN2076127.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(10639..13089)
                     /gene="parC"
                     /locus_tag="U4B19_000359"
     CDS             complement(10639..13089)
                     /gene="parC"
                     /locus_tag="U4B19_000359"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEN2076128.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(13101..15146)
                     /gene="parE"
                     /locus_tag="U4B19_000360"
     CDS             complement(13101..15146)
                     /gene="parE"
                     /locus_tag="U4B19_000360"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEN2076129.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(15312..15746)
                     /locus_tag="U4B19_000361"
     CDS             complement(15312..15746)
                     /locus_tag="U4B19_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEN2076130.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            15873..16511
                     /gene="plsY"
                     /locus_tag="U4B19_000362"
     CDS             15873..16511
                     /gene="plsY"
                     /locus_tag="U4B19_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEN2076131.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(16643..17518)
                     /locus_tag="U4B19_000363"
     CDS             complement(16643..17518)
                     /locus_tag="U4B19_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEN2076132.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(17600..18391)
                     /gene="codY"
                     /locus_tag="U4B19_000364"
     CDS             complement(17600..18391)
                     /gene="codY"
                     /locus_tag="U4B19_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEN2076133.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(18409..19809)
                     /gene="hslU"
                     /locus_tag="U4B19_000365"
     CDS             complement(18409..19809)
                     /gene="hslU"
                     /locus_tag="U4B19_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEN2076134.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(19823..20371)
                     /gene="hslV"
                     /locus_tag="U4B19_000366"
     CDS             complement(19823..20371)
                     /gene="hslV"
                     /locus_tag="U4B19_000366"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEN2076135.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(20408..21313)
                     /gene="xerC"
                     /locus_tag="U4B19_000367"
     CDS             complement(20408..21313)
                     /gene="xerC"
                     /locus_tag="U4B19_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEN2076136.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(21511..22887)
                     /gene="trmFO"
                     /locus_tag="U4B19_000368"
     CDS             complement(21511..22887)
                     /gene="trmFO"
                     /locus_tag="U4B19_000368"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEN2076137.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(22856..24934)
                     /gene="topA"
                     /locus_tag="U4B19_000369"
     CDS             complement(22856..24934)
                     /gene="topA"
                     /locus_tag="U4B19_000369"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEN2076138.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(25056..25913)
                     /gene="dprA"
                     /locus_tag="U4B19_000370"
     CDS             complement(25056..25913)
                     /gene="dprA"
                     /locus_tag="U4B19_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEN2076139.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(25969..26736)
                     /locus_tag="U4B19_000371"
     CDS             complement(25969..26736)
                     /locus_tag="U4B19_000371"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEN2076140.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(26729..27589)
                     /gene="ylqF"
                     /locus_tag="U4B19_000372"
     CDS             complement(26729..27589)
                     /gene="ylqF"
                     /locus_tag="U4B19_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEN2076141.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            27849..28247
                     /locus_tag="U4B19_000373"
     CDS             27849..28247
                     /locus_tag="U4B19_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2076142.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            28526..29026
                     /locus_tag="U4B19_000374"
     CDS             28526..29026
                     /locus_tag="U4B19_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN2076143.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(29109..29660)
                     /gene="lepB"
                     /locus_tag="U4B19_000375"
     CDS             complement(29109..29660)
                     /gene="lepB"
                     /locus_tag="U4B19_000375"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN2076144.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(29812..30039)
                     /locus_tag="U4B19_000376"
     CDS             complement(29812..30039)
                     /locus_tag="U4B19_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEN2076145.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(30036..30554)
                     /gene="msrA"
                     /locus_tag="U4B19_000377"
     CDS             complement(30036..30554)
                     /gene="msrA"
                     /locus_tag="U4B19_000377"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2076146.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(30574..31287)
                     /locus_tag="U4B19_000378"
     CDS             complement(30574..31287)
                     /locus_tag="U4B19_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEN2076147.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(31290..32186)
                     /locus_tag="U4B19_000379"
     CDS             complement(31290..32186)
                     /locus_tag="U4B19_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2076148.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(32344..33186)
                     /locus_tag="U4B19_000380"
     CDS             complement(32344..33186)
                     /locus_tag="U4B19_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2076149.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            33318..33971
                     /locus_tag="U4B19_000381"
     CDS             33318..33971
                     /locus_tag="U4B19_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEN2076150.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(34034..34561)
                     /locus_tag="U4B19_000382"
     CDS             complement(34034..34561)
                     /locus_tag="U4B19_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2076151.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(34581..35528)
                     /locus_tag="U4B19_000383"
     CDS             complement(34581..35528)
                     /locus_tag="U4B19_000383"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEN2076152.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(35540..37426)
                     /locus_tag="U4B19_000384"
     CDS             complement(35540..37426)
                     /locus_tag="U4B19_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2076153.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(37586..39664)
                     /locus_tag="U4B19_000385"
     CDS             complement(37586..39664)
                     /locus_tag="U4B19_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEN2076154.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(39677..39952)
                     /locus_tag="U4B19_000386"
     CDS             complement(39677..39952)
                     /locus_tag="U4B19_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076155.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(40314..40769)
                     /locus_tag="U4B19_000387"
     CDS             complement(40314..40769)
                     /locus_tag="U4B19_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076156.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(40923..42131)
                     /locus_tag="U4B19_000388"
     CDS             complement(40923..42131)
                     /locus_tag="U4B19_000388"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEN2076157.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(42243..42575)
                     /locus_tag="U4B19_000389"
     CDS             complement(42243..42575)
                     /locus_tag="U4B19_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN2076158.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            42723..43598
                     /locus_tag="U4B19_000390"
     CDS             42723..43598
                     /locus_tag="U4B19_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2076159.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(43680..44261)
                     /locus_tag="U4B19_000391"
     CDS             complement(43680..44261)
                     /locus_tag="U4B19_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEN2076160.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(44267..45526)
                     /locus_tag="U4B19_000392"
     CDS             complement(44267..45526)
                     /locus_tag="U4B19_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN2076161.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(45528..46574)
                     /locus_tag="U4B19_000393"
     CDS             complement(45528..46574)
                     /locus_tag="U4B19_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076162.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(46809..47084)
                     /locus_tag="U4B19_000394"
     CDS             complement(46809..47084)
                     /locus_tag="U4B19_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEN2076163.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(47327..48637)
                     /gene="der"
                     /locus_tag="U4B19_000395"
     CDS             complement(47327..48637)
                     /gene="der"
                     /locus_tag="U4B19_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEN2076164.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(48819..50048)
                     /gene="rpsA"
                     /locus_tag="U4B19_000396"
     CDS             complement(48819..50048)
                     /gene="rpsA"
                     /locus_tag="U4B19_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEN2076165.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(50183..50863)
                     /locus_tag="U4B19_000397"
     CDS             complement(50183..50863)
                     /locus_tag="U4B19_000397"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEN2076166.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(50912..51547)
                     /locus_tag="U4B19_000398"
     CDS             complement(50912..51547)
                     /locus_tag="U4B19_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2076167.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(51552..53015)
                     /locus_tag="U4B19_000399"
     CDS             complement(51552..53015)
                     /locus_tag="U4B19_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEN2076168.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKTWNIDFLGQDTFSYGIIHTIEFRR"
     gene            complement(53002..54033)
                     /locus_tag="U4B19_000400"
     CDS             complement(53002..54033)
                     /locus_tag="U4B19_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2076169.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            54064..54285
                     /locus_tag="U4B19_000401"
     CDS             54064..54285
                     /locus_tag="U4B19_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEN2076170.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(54450..55187)
                     /locus_tag="U4B19_000402"
     CDS             complement(54450..55187)
                     /locus_tag="U4B19_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEN2076171.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(55340..56680)
                     /locus_tag="U4B19_000403"
     CDS             complement(55340..56680)
                     /locus_tag="U4B19_000403"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEN2076172.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(56917..58440)
                     /locus_tag="U4B19_000404"
     CDS             complement(56917..58440)
                     /locus_tag="U4B19_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEN2076173.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
BASE COUNT        16076 a        12361 c         9996 g        20251 t
ORIGIN      
        1 atataaactt tcggatagag tataaaataa ggaaacgccg cagcgtttcc tttccaactg
       61 tagccgaata taaatttgta tatcacttat ttaaaatagc tattttctcc caaagagttt
      121 ccttgatcat cgatcaatcc ttgtaatgag cgttctaaga taccatggct ttttttcgat
      181 tttttttctt ctgactgctt tgtttcctct aacgtttttg ttcgattgaa tgttcgtaca
      241 gacggctcac catttctttt ttcttgatac ggcgtcggtt ctgtatcgaa caacagataa
      301 gagtcatccg tttttttcat cgattcgatt aaatgatcct cagaaatttt gctttctcta
      361 gggtctggga taatcgatgc gggtacatat ttaggaacaa aataagaacg ctcccgcacc
      421 ttcgtgtcaa cagatgcgtt tcgtgtagta tgcttggact ctgtccttgt ttcactttgt
      481 ctgacagaac gagaggaatc accaaaaagt ttttttcgtc ctgtcgatgt gatttctgat
      541 tggtaatggc cggtataatc cggtaatttt cctttatgct tttccaaatt ttccttacgc
      601 ggagctgact tcaatccatt tgttgcacga cgcttgcgac ggattttctc ggtgttcgtg
      661 ttcgtgaaat gaaattcttt catctgttca aacggggact gattctgttc agtcaaaaga
      721 tcttcttgtc cgtcaaataa tactcgcttc ttgtcgttgg ttaattttac gcctaactca
      781 tcatcgtacg caggaaaacg gaaatgtttt cgtctgattt ctttcattca aaccatcctt
      841 ctcaaaaatg gaacttttct caataatagc acagattcat tgttttcagt agcaatgcgc
      901 tgattatttt aagaaaatag cgatgaattt cataaatata tatatagtaa taagacttaa
      961 ttcgttgtct ctctgataac tagttcagga aaaaaacgac gataaccttg actcgcccct
     1021 ttttcttcta ttttttttaa caacatttct ccacatactt cacccatacg tataatatct
     1081 tgcttgatcg ttgtcaattt aggtgaactg atttggtcta aaaatacccc atcaaatcca
     1141 atcacaccaa attcttcggg caagtgtcct ccagcattta taatccctct tactgctcct
     1201 agtgctagcc gatctgaact acatacaaaa caagttttat ctgtaaaatc tgcccagttt
     1261 tctgtcatat atctttctac ttctgtcgaa tgatttggaa accgcaagat tttactagga
     1321 agatcaactt ccttcatcac agataaataa ccagtttccc ttgaaatttc aaaaggctcg
     1381 tccacttcga taccgatata aataatcgtt tgataatctc tttctaaggc atattgcgtt
     1441 gcttttgctg taccatattc attatcgctg tctacataat catatccttc agaattttcc
     1501 ccaaacaaga caactggttt ttccaaacgg tcgatccaat cgaagtcttt ttttctgaca
     1561 cctgtgatga tgtatccatc acaagaaccg acgtcgaagg catcatttgt aactaattgg
     1621 agagaataat gcgctttacc gatccgttta gcgattccca tcagcaaatt catgtaatag
     1681 ggctcagttg tgtcgatttc ttccagtata aataatttga tgatctgcga ccgattcgtg
     1741 acaagtgctt ttgcagcaat atttggacgg taatccagtt cagccattgc tgagaaaact
     1801 aactctttca attcgtcagt cacctgttcc ggatgattga tgactcgaga gaccgtcatt
     1861 tttgaaacat tggcacgttt tgcaacatct gctaaagtgg gcatgctctc ttcctttcta
     1921 acatgttgtc attcttacta tcaattatac caaattttcc taacagagtt tattatagcc
     1981 caaaataaaa aacggaaaat cctctatcgt catgagtgat tttccgtttc aattatttat
     2041 acttttttat caaatttttt gctaaaaaag tggaagcttc ttccattagt tgtatttgta
     2101 ccatatgtcg ctgtgttttt gaggaacgaa aaacaatttc ttttccgtaa gacttatgcc
     2161 gatttttttc tacaaatcgt tccgtttgta tataagggat tttttcgtca gcttgtccat
     2221 gccaaaagaa cagaggacgt ccagctaact tttccggctg caatgacaaa tcatagctct
     2281 taacccatga aatcaaatca aaatagtctt ttggtacgac ataattatat tccgatgcgc
     2341 gccttacgat ttcttttcca tattccaaag gtgcagggga acccatcaaa cacacgcccg
     2401 ctactatatg aggatgttta gccaacaagg cgcatgttgt cattcccccc atcgaaaccc
     2461 cgccaactgc aagctgatca tgtagaagac ctcttttttc taaatgcttg ataagtgtgt
     2521 caaattcaaa cagatttccg tagatactat cccaaaatgt ataggatgga atagaagaaa
     2581 ccggctgttt gcgctcccca tgattcatcg catctggcag gatcacacga atgccctgct
     2641 gcgcaatttt tcttccttgc gtcaaaacta actccttact tgtctgccat ccgtgataat
     2701 agatgacaac gggaatacgt tcctctctta attctttcag aacaacttct aacactggaa
     2761 tatgatggac agaacgtttc aatataattg gattcaattg ttactcacct attttcatta
     2821 aatttcctga tcgccatagc aaccatgctc caacagttgt ctggaacagg ttcagtgtac
     2881 caaataatat aaatgcagga ctgaatggct cgggaaataa gcacagagaa caaagtccca
     2941 ccactcctaa tgctagcgaa aggagtggat aaatagaaaa attcaaaaaa ctggcaacgc
     3001 taccgaaaat attaccaata aacagtatca aaaagaaaaa aacgcttaaa attaaaccat
     3061 ctgtcagcga catctgaaaa acaatgttct ctacctcaat tggctgttga atcgaataga
     3121 cacctaagta tatactgaga aggagactta gcacgtggct ggcacaaaac acacgattga
     3181 ccgttttcat tttaatcacc taacctatct ttataattct attataatca ttttatcatt
     3241 tttttgtatg tataaagttt caatttttct tcacttacga ttttttttga tattgttgtt
     3301 atataaatga aaggagtttt acaaatgatt gatccaaaaa tgttggggat gatcttcctc
     3361 attaattttg cttatatcac tctgaacacg ttacgtttca tgttgacaat gaaaggctat
     3421 cgactgattg ctccacttgt cagtatggtc gagatcacga tttatatcgt cggcttatcc
     3481 cttgttttag atcgtttaga taatccactg aatttatttg tctatgcgtt aggttacgca
     3541 gtaggtatca gtgtcggaat tttgattgaa gacaaattgg cattaggata catcatggtc
     3601 acaacgattt tgccagcaaa tagcgcagaa gataaaagtt tgccgggtat actgagagaa
     3661 cacggttatg gtgtgacaca gacttctgca atggggctgg aaggtgagcg gctagttctc
     3721 gaaatacttt ctcctagaaa aagcgaacgt gaactgtatc gcctgatcaa agaaatcgaa
     3781 gagcgtgctt ttatcatttc ttatgagcca aaatatattt caggtggttt ctggactaaa
     3841 aaagtgagaa aacgacaagg aaaataactc acaaacttaa aaactgtcta atatctaaat
     3901 taaattttaa atgttagaca gtttttattt attattttga ttaaaaaaca tcgacatata
     3961 ctcatcaatt tgttattata ctttatatac ttatcaattt aatttggaga tattatggaa
     4021 attcaacgta gaaccactaa ttttttacat ttaggctctt tttttgcaac tttgacgatc
     4081 ttattttttc tatgttattc ataccttaag acctcacatg cgccttctag tgcattaggc
     4141 tggttgcttt tggatcattc tgatatcaca gaagccgaag aaaatccgtt ttttttgatt
     4201 ttgtcttctc tttgcggctt actgttttct gtttatttcg gtgcctattt ttttcatcga
     4261 catcctggta cgttaaacga agcttcaagc gtattgattg catttaaaac gttttctttt
     4321 ttagcaattc ttttttattc gtttaatcac aatacgcttt tcttctttat catgaatggt
     4381 ttagtcgtga ttttatccat ttttacttat tttatttttt cactcatttt tagccaattg
     4441 aaatgtccgc tcttctttcg attagttcct gtcacctgtt ttatttacag cttgttttcc
     4501 ttttttgtcc ttgctttaat gccactgacc aacccttcga cgatccaagt cgcaggaaat
     4561 ctgttggata tgctcttcat cgtcagtcta ggtgtattta tgtggcaaag aaaaaacaaa
     4621 caaaataaaa agcattttga agcctgacta ttacgacttc ataaatgctt ttttagtttt
     4681 aaacacttca atctaaaaga ataacccaag attggataaa ttattaatga ctcgccattc
     4741 gtttcttagt gattctggat tatcaaatgg acttcctaat ctaatcagct agttcttccc
     4801 ataaaatgcc atacttgact gaatcataat attgattatt taaaaatcta acttttctta
     4861 tcactacttc ctttgtcatt ccacatttct caccattttt ttgcattcct ttgtttccag
     4921 accatgtagt gaatctaata ggtggtagat aggcaaaata ctcaaataat tcttttaacc
     4981 ataaaggaag agaaaggctc ctcctgcaag agtcagtata atttttgctt actgctctct
     5041 taggttttac tcactaaaac tgttccatat tctttgatat aagaaaatgc atgcggtata
     5101 aatttcttgc tcagctcaga aaagatttgt cgtttaagaa aggccatttc ctccattgtc
     5161 aatgaaggat ccaatgttgg taattcaatc tcaaaattgt ttttttcgac aataggtaat
     5221 tctaatgtga atgattcttc ttcctttata gtattcgtca caagtagaga ctgatggaaa
     5281 atactaggcc ggcgattttt atatttattc ttacgtatcc tgtcaacgat aacttctatt
     5341 tgatcaaaaa agtacgattc atttacgtct ccaataagtt cggctaattc tttgaatgcc
     5401 ttcgttacag gaagctgatt cttctgtaca aaaaagtaat tagccaaagt aagcgggaag
     5461 tgttctatag aggaaagtgt gataaaacgg tacttctcat ttcctgcagt tgtcctcagc
     5521 aattctgtct ctcggctgtt tcttactcca taaaaatacg cttctttccg ttcatcttcg
     5581 aaacaatacg cccaaataac ttctcccatc gctaattttt gttcatcaac agagagtctc
     5641 ttcaattgtt cagctatggt ttcagcagcc gaagtggctt gttgcaagta atctttagga
     5701 tcaaatattg gaaagttcaa atgatatctt cgttcttccc tcttgattaa tcctgcatcg
     5761 atcaataaat caagaaaatg ggaaaattta ttttgcgaaa attctttttt caattctctt
     5821 agaatcgttt cttgttctcg atgttcatat agataatcgg tcaatgaaga aaaaagcgga
     5881 tcatcggtca gtttttgata aaagttttcc ttattttttg aataatagtt catatatgtt
     5941 ctcttttcta tccgttttat tttacttaaa aggcttttac gtaaaaagga ggaagaacaa
     6001 gagtcataat tgactgttat tctcctccat ttcttcggcc tttagtatgt tctatgtttc
     6061 tttttatatc aatcgcttat ccattcattt tgaatgcttc atactaccat ttgattagct
     6121 aaatacagcc gtcaattgag gtacgacttg ttttttacgt gaaacgacac ctttaagcaa
     6181 agcacggttg ttggtcaaag ttgtttggaa agcttcttct actttttctt ttcctttacc
     6241 agcaacaagt aattctgaat cactgtctaa gatgtttgtg acaactaata cgaacagatc
     6301 gtagccattt gctttcattt catcttccat tgctgcttct agctctgctt gtcgatccat
     6361 cacttcatta aggtccactg tattgacttg agcgatacga acgttggaac tgcccattgg
     6421 gaaacttttt gcatccaaat caagaagaac cgcagctgat ttatcactta gatttgttcc
     6481 tgctttcaac atttcaagtc cataagtgtt tagatcgact tcagcgattg ctgcaagttc
     6541 ttttgcagct gcttcatctt caggcgtaca tgttggtgat ttgaacaaca atgtatctga
     6601 aatgatagct gagagcatga ttcctgcgat ttctttagga attgttactt ggttttcttt
     6661 atatagtttt aaaataatcg tacttgtaca tccaactggt tctgcacgat agtacaatgg
     6721 attagctgtt tcaaagtttg caatacgatg gtggtctact acagctaaaa tatttacttc
     6781 gctgatatct gaaatacttt gttggaattc attatgatcc acaagcatga catctgcatt
     6841 ttctgcttta gctgactcta ctactcgtgg tgcagatagc tgaaagtggt ctaatgcgta
     6901 ttgtgtttct tcattaggtg tacctagagc aactgcttct gtttctttac ctaattcatt
     6961 ttgcaagtgt gaaaacgcaa tggctgcccc gattgcatct gtatctggat tttggtgacc
     7021 aaaaactaaa atttttgaca tgtcattcgc tcctttgtga tttctcctat ttaataatag
     7081 caaaaaaacg gaaagaagca atacttatct tctttccgat gcgacctatc gcgttaaata
     7141 ttttgtatac tcttctgtat gaagcaatcg tttagcattt tcgacacgtt cttttgtagg
     7201 tggttcgatc ccttctaatg gatatttcaa accaagtgtt tcccacttgt attttcccat
     7261 cgtatgataa ggaagaatct ctactctgtc tacatttgac aaggtttgaa taaagttgtc
     7321 tagtcggatc aaatattcat cataatcact tcgctcagga actagtacat ggcgaatcca
     7381 aaccggtttg ttgatttttg acaaatattg agccatttct aaaatatttt cattgtccca
     7441 atgcgttagt tttttatgct cttcattatc gatgtgtttg atgtcaaata acagtaaatc
     7501 tgtatacttc atcaattctt gaaaacgtga aaagaaaggt tcttcatacg taaaaggttt
     7561 tccgcatgta tccaatgtag tatgcatctt ccgctctttt gcttttttaa atagttcgat
     7621 caaaaaatcg atctgcaata gaggttctcc accactaaca gtaataccac ctttatctcc
     7681 ccagtaaggt ctgaagcgtt ctgctttatc taaaagttca tctgctgtgt attctttccc
     7741 tccgccaata ttccaagtat ctggattgtg acaaaattgg caacgcatgc ggcatccttg
     7801 cataaacacg acaaagcgca gtccaggtcc gtctactgaa ccgaaagttt cgatagaatg
     7861 aacatagccg attgtttttt cttccattta agttcacatc ttttctttga tttcatttta
     7921 agtacaagtt agctgtcgaa gcgaagcttt ctgaacaaaa tttttcgcaa ttgccgcttc
     7981 gttatgtgta aaatttacag ggacggaaca tacaagtcct gcccctgtaa atcgtttctt
     8041 tcaaataagc atactgatta ttcgattttg atgcgatggc tattcaacgt cctttcgacg
     8101 ctaagtcgaa aaactttttt cacgaacttt ttacattctg tcgtgtgaag ttctcatcaa
     8161 gacatctaat tgttgttctc ttgtcaagtc acggaattta actgcataac cagatacccg
     8221 gatcgtcaag ttcggatatt tcattggatt ttccattgca tcacgcaata aatcattact
     8281 gaatacattg acattcaaat gataagctcc ttttgagaag tatccatcta agacattacg
     8341 caagttttcg attcttgtat cttgttcttt tccaagacct gaaggattga ttgtttgggt
     8401 atttgaaatt ccatccaatg cacagccgta attcatttta gctgttgagt tcaggctagc
     8461 taataaccca ctttgttccg cattgtaact tggatttgct cctggcgcaa gtggttttcc
     8521 agcttcacgg ccatcaggag tatttcctgt atttttaccg taaacaacat ttgaagtaat
     8581 cgtcaataat gaagttgttg gttttgagtt gcggtaagtt ttatgacgtt tgatttgtcc
     8641 catgaagtat tccaagatcc atgaaactaa ttcatctgca cggtcatcat catttccgta
     8701 tttagggaag tcgccatcca cttcaaaatc aactgctatt cctttttcat tacggattgc
     8761 ttttactttt gcatatttga tcgctgacaa gctgtctgcc gcatgagaga tcccagcaat
     8821 acctgtagca aatgtgcgtt gcaagtcaga atccatcaaa gccagctgag ctgcttcata
     8881 agaatattta tcatgcatat aatgaatgat gttcaacgta ttgacatata gttctgctaa
     8941 ccaatccatc atattcatga agttatgttt cacttcttca aagtctagta tttctgatgt
     9001 gattggacga tattctggtc caacttgggc gccagtcact tcatcgactc cgccattgat
     9061 ggcatataat agtgttttag ccaagttagc acgagcgcca aagaattgca tgtcttttcc
     9121 gacaactgtt cctgatacac agcaagcaat cgcacaatca tcagaacccc attgttcacg
     9181 aagctgatta tcattttcga attgaatcga agaactttcg atagcgattc tgctagcata
     9241 tgttctgaat ccttctggaa ggtgttcaga atacaaaaca gtcaagtttg gttctggtga
     9301 aggtcccata tttgttaatg tatgaagcag acggaaatca ttctttgtaa ctaatgaacg
     9361 accatctaat cccatacctg caatggataa agtagcccag atagggaatc ctgagaataa
     9421 ttggttatat tcaggtgtac gcgcgaattt gaccatacgt aatttcatta ctaagtggtc
     9481 gatcaattct tgtgcttctt tttctgtgat tactccatta tccaaatcac gttggatata
     9541 gatatcaagg aatgctgaga tacgtccgat tgacatcgca gcaccatttt gtgatttgat
     9601 tgctgctaaa tatccgaagt atagccattg gacagcttct tttgcatttg cagctggttt
     9661 agaaatatca tagccatatt tcgcagccat ttctttcatt tgcgctaatg cacgatattg
     9721 ttcagtgatt tcttcacgta aacgaatcac ttcttcactc attgtatgat aaccagtcat
     9781 atcatgatct tttttctttt gttccatcaa atagtcgatt ccatacaatg ccacacgacg
     9841 atagtcaccg atgatacgtc cgcgaccgta agcgtctgga agccctgtaa tgattttgtt
     9901 tttacgagca gcacgcattt cagcagtata agcgtcaaat acaccttgat tatgtgtttt
     9961 gcgccagtca gtgaaaatat gtgtcaattc atcgtttggt gtatagccat tgctttttag
    10021 cgcattgtta gccatattga ttccgccgaa cggcatgaat gcttgtttca aaggtttgtc
    10081 tgtctgtaga ccgacgattt tttccaaatc tttatccaaa tagcctgcat cgtgtgaagt
    10141 aatagttgat acgatttctg tgtccatatc atagacaccg ttacgctcga attggatatc
    10201 atttaacttt tgcaattcat cccataattt ttctgttgct tctgttgggc cttccaaaaa
    10261 gctgtcatca ccgcgatatt cagtgtagtt attttgaata aagtctctag tgtctacgcc
    10321 ttctttccat ttggtacctt taaatccatt ccattgctcc attgtatgga cctcctaaat
    10381 ttctgtttta tgtaacagtt tttacgtgat tagtataaca cagcacccta tctttgcaag
    10441 ggttttcttg ttttttattt aacaattttt atgttttatt tacatatatt taaatcaacg
    10501 ttgacatacc aacatttaag cattgtgaac actttcatct ttttttgcaa acgtataagg
    10561 atttattaaa aatgaatgaa aaagagacgc cacctgtatt aatacagatg gcgtctcttc
    10621 attaaaatac tacaacagtt acgactcgat gaaaatctca ggagcatcga taacttcgat
    10681 gacttctcca ccttttttct catccatcgc aaatgatcca ttcgatgttc gatcacctat
    10741 agggaaatca gttgggtcaa tcgttaactg ctgccctttt tgattcatca aggttatttc
    10801 ttttgatctt ccttccgaca tatagataat acggtgcgga ttttttttca gctcacgcat
    10861 gaccgtcact ccccgcttcg cacgtgaagt ttggctgatt tcttgagcaa gcatgcgttt
    10921 aacagctcct cgctgagtaa cgattacgat cggcgtatca ccttctgcta acacaagcag
    10981 cccgttgata atcctatctc catctttcaa attcatagac ttcactccag ctgcctttgc
    11041 tccaacaacg ggaacatctg ttaaaggata gcgcaaaccc attccttgat gcgttactaa
    11101 gaaaacatct aagcctggct gatcttcttt cagatacaca gcaatcagtt catcttcttc
    11161 cgcctttagc ttcatacagc tgagcggacg gctcttatag gtgcgccatg gttcgaactc
    11221 tgtcattcgt gtttgcttga tcaatccatt cttgctgatg aatacaaatg ttttctctgc
    11281 ttgaattttt tgatatggga aaacagctaa aatcgattcg tctacagaca gattggagat
    11341 tgtttgagaa atgtgctcac cagcatcttt ccatttcaaa tcgggtagtt catggacagg
    11401 gcggtagatc acatttgctt tatttgtgat cagaagaaca tggtccaatg tactcaattc
    11461 acctgagtat agcaagtagt cgccttctcg catcccgatt tcttctggtt tggaagcact
    11521 ataagaacga aggcttgttc gcttcacata gccttctcgc gtcacactga caattacgtc
    11581 ttcttgtgcc actaagactt gcgtatcaat tttgatttct tcgatttcgt cttcgattgt
    11641 cgttagacga gcggaagaat aatttttttt cacttctcgc aattcttttt tcataacaga
    11701 gaaaagctct ttgtcatttg aaaggatttt attcaactct gtgatttgcg caatcaagct
    11761 ttcggattcc ttttgaagct ctgtgatatc cgtgttcgtc aaacggtata attgcaatgt
    11821 gacaattgct tctgcttgtt cttctgtaaa ttgaaagacg tcgactaaat tctttttggc
    11881 gtcttttttg tctttacttt cacgaatcgt tgcgattact tcatccagaa tcgataaagc
    11941 tttcatcaat ccttctacga tatgctgtct tttttgagct ttggctaaat cgaattgact
    12001 acgttttgtt atgacttgct ttcgatgctc gatataactt cttaaaatgt ctttcagtcc
    12061 aacttgatgc ggagtcatat ggtcgatagc gaccatattg aaattgtaat tgatctgtag
    12121 ctcagtgttt ttgaacaaat aattcaaaat tccttgagca ttggcgtctt ttttcaattc
    12181 gacgacaatt tgcagacctg ttctatcact ttcatctcgt acttcagcta ttccatcgat
    12241 tttcttattc aaacggatct catccatttt tttcaccaag gtcgctttat ttacttcata
    12301 tgggatttct gtgatcacga tttgctgttt attacccttc attgcctcta tctttgtttt
    12361 cgaacgaaga ataacttttc ctcgtcctgt ttcataagct tttttgattt catcttttcc
    12421 ttgcaagatc cctcctgttg gaaagtcagg tccaggaata tattccatca atttttcaag
    12481 gctcgcttgt ggatgatcga tcatatagat tgtgccatcg atcacttcag ctaagttatg
    12541 agtcgggatt tctgttgcat atccagcaga tatacctgtc gatccattga cgagaaggtt
    12601 tgggtatcga gctggaagga ctgttggttc tttttctgta tcatcaaaat tccacacgag
    12661 atcgactgtt tctttctcga tatctgctag catctctcca cttaacttgg ataatctggc
    12721 ttcagtataa cgcatcgcag caggtggatc accgtccata ctcccgttat tcccgtgcat
    12781 ttcgatcagt acttcccgca acttccagtc ttggctcatg cggaccatcg cttcataaat
    12841 actgatgtca ccatgaggat gataattccc catgatattt ccgacagatt ttgctgattt
    12901 acggaatcct ttgtcatacg tattgccatc tttgttcatg gcaaataata tccggcgttg
    12961 cactggcttc aatccatcac ggatatcagg cagtgcacgt tcttggataa tatatttgga
    13021 ataacgtccg aagcggtcac ccatcacttc ttctaaagtt aattcttgta cttcttgtcg
    13081 attttccatc aggtgacctc ctattctact tcaaataact gattgttttc atttttatct
    13141 gctcgttctt catctaataa atcattagaa actgaaggag agatttcagt atcctctttt
    13201 ttatctaaga tactgccatc ttcttcgagc gtaaattgca cgtgattttc gatccatttt
    13261 cttcgaggtt ctactttgtc acccatcagt gtggttactc gacgttcagc ttgcgcagca
    13321 tcatcgatac ggacgcggat caacgtacgg gaagtcggat ccatcgttgt ttcccacaat
    13381 tgttccgcat tcatctctcc aagtccttta tatcgctgca gcatatatcc tttgccgact
    13441 tttttaatca ttgctgccaa ttcgtcatct gtccacgcat attcaatgac ttgcttcttt
    13501 ccttgtcctt tggatacttt atataaaggc ggtaacgcga tatatacttt cccagcttca
    13561 atcagcggtt tcatataacg ataaaagaaa gtaagcagta atacttgaat atgggcgccg
    13621 tctgtatccg catcggtcat gatgattact ttgtcgtaat tacagtcttc gatagaaaac
    13681 tcaggaccga ctcctgcacc aatcgtatag atcatcgtat tgatttcttc atttttcaag
    13741 atatcttgca ttttagcttt ttcggtattg atgacttttc ctcgtaaagg tagaattgct
    13801 tggaactttc tgtctcttcc ttgtttagca gatccgccag cagaatcccc ttcgactaaa
    13861 tacaattcat ttttcttagg gtttcgggat tgtgcgggtg tcaattttcc tgaaagaaga
    13921 gattcgcctt ttttacgctt cttcccattt cggctttctt ctctggcttt acgtgctgct
    13981 tctctcgctt ctctggcttt gattgcttta cggaccaaca tctggctcat ttcgctattt
    14041 tcctgcaaat aaaagcccat ttgttcactg atgacgttat ctactacggt acgagcaact
    14101 ggcgtaccaa gtttttcttt tgtttgccct tcaaattgca gaagattttc aggtacacgg
    14161 atagaaagta cagcagctaa cccttctctg aaatcactac cttccagatt cttgtcccgt
    14221 tctttcagca aaccaacttt tctcgcatat tcattataag atttcgtcat cgccgctttc
    14281 ataccagctt catgggttcc cccatctttt gtccgaacgt tattgacaaa agataaaaca
    14341 ttttctgaat aaccgtcatt gtattgataa gcgacctcta cttcgatacc ttctttttct
    14401 cctgaaaaat aaacaactgg tgtcaatgta tctttttctt cattcagata atcaacaaat
    14461 tctttgattc cttcttcgta atgaaaaatt tccttgactg gttcttctcc acgaagatca
    14521 gaaagagaga ttttaacgcc ttttagaaga aaggcagatt ctcttaaccg ttcggctaaa
    14581 atttcatatg aaaatttcgt tgtactgaat attgtatcat ctggtaaaaa atggacagtc
    14641 gtcccgtttg ctttttttgt ttttcctatc tttttcaaag taccgtcagg tttaccgccg
    14701 tttttaaatt tctgctgata ctctacgcca tctctaacga tcgttacaat tagccatttc
    14761 gataaggcat ttaccacact ggctcctaca ccgtgcagtc caccagaagt tttgtaccct
    14821 ccttgaccaa actttccgcc agcatgcagc acagtaaaaa tcacttcaac tgttggtatt
    14881 ccggaagcat gcattcctac aggcatccca cgtccgctat ctgcaacagt tatactattg
    14941 tcttcatgga tggtcacatc aatctcactg ccataacctg ataatgcttc gtcaactgca
    15001 ttatcgacga tttcatagac taaatgatgc aagcctcggc tgtcagtgga tccaatgtac
    15061 atccctggtc tttttctaac tgcctctaat ccttccaata cttggatgga cgagtcattg
    15121 tattcgttgt ttactttttt tgccaaggca aacgctcctt caataatact ttgcggcaaa
    15181 accgcaccac gattatcata ctagaaaagg tcgtgaaaaa aaagagggta gacgagaaat
    15241 caattttaat tatctcctaa ccgctttttc gacattcccc tctatcgatc atctcttctt
    15301 tgtggtcatt tttatttttg gttttctttc aacttaccaa gttcaatctt aatacaacga
    15361 tccattacga tatctgtacg tcctgctttt cttagcatct cagctgcttc tttgttttca
    15421 atccctaatt gtgcccagaa cactttagca tcagttttca aaaaatcacc tgctatttca
    15481 ggtaaaaatt cactccgtct aaatacatct acgatatcga ttggatcatc gatttctgtc
    15541 aaattcgcat agactttctc gcctagaatt tcttcaccag ccaataatgg atttactggt
    15601 atgattttat atccattgtc ttgcaatacc tgtgcaacca tgtaacttgt tcgttctggt
    15661 ttattgctca agcctacgac ggcaatattt tttgcttctt ccaaatagtt gaaaatcgtt
    15721 tcttgtgttg gattttttac agacatttct gccacccctt gcttttcttt ttattccatc
    15781 tattatactt gtatctttca ttttttcaat tgttcctgtt acctattcat gctaaaataa
    15841 aggatagttt tcaacttgaa agagggtaaa aaatgaaatt gatcttatta cttattttag
    15901 catatctttt aggttctatt ccttccggtg tctggattgg caaattgttt ttcaaaaaag
    15961 atatccgaca acacgggagc ggtaatactg gaacgaccaa tacatttcgt gtattaggca
    16021 aaacagccgg gattgttgtc ctgcttatgg atattttaaa aggtacattg gctacttgtt
    16081 tgccgatgat cttccacctt tctgggatta accctctttg gtttggggta tgcgccattc
    16141 ttggccatac atttccgatt tttgctgggt tcaaaggagg aaaagctgtt gcgacaagtg
    16201 caggaatgct tttaggattt aatcctgtat tttttgttta ttcttgtatc atctttatcg
    16261 ttagtttatt ttgtacaagt atggtgagtt tgagcagtat gatcagtgca gtattgatca
    16321 ctttatctac gatcgtcctt ccttatgtag ctcctgtgat tttggctaag cccaattggt
    16381 tactgacgat cattgccttt ttagtttctt cctttatctt ttatcggcat aaagacaata
    16441 tcaagcgtat cagaaaccat actgaaagtc gaatcccgtt tggtttgaat agcagcaaga
    16501 aaaaatcctg atactttttc attccaaaga gataaaacgg gagtgtgaca aatcgatgtc
    16561 acactccctt attacttaca ggatagcttt tttacacacc ttggtaaacg gtgttccctg
    16621 ctaacttctc ggtcacaacc tcttacttca ctgtgatgga atattttgtc ttgaagattt
    16681 catgggcacc tagttgattg atccctactt tttccatcaa gtttccacta ctgttcaccg
    16741 tgtcagcgat tccgcaccac ggttcgatac aaacgaaagg cgcttctttt ggataaggag
    16801 accagatccc tacataaggc atatctttat agcttaaagt tacgctatgg gaagattttt
    16861 cacttcgaat ggcgtacgca tttagacccc ttgtttcaaa aaccaaggca tcttgctcaa
    16921 aaagttcacg attcaaagcc agattcgtat ttgtctgacc taacgtcttt tgatccatat
    16981 cgatgaatgg gcctttcaat ggtaggcgta atcttgattt caatggagaa aatgccagat
    17041 agaaatcttc aaatgacaat ccttcttcaa gaggtacatt gaaagcagga tgtccaccga
    17101 tagaaaagaa catttctttt tcaccggtat ttgccacttg ataatgaacc gtgatgccgt
    17161 ctcctcctaa ttcatagctg atcactaatt caaaatcaaa tggatacttt tctaatgtct
    17221 ccggcgtact ccttaagatg aaactagcca tttcttgtcc atgttcaatg acttcaaaat
    17281 ccatatcccg agcaaatccg tgttgcccca tttgatatgt tttcccttga taaacatact
    17341 gatcatcttt taaacgtcca acaaaaggga ataaaaccgg tgcatgtctg ccccaatacg
    17401 ctggatcacc ttgccagata tactctaaac catgttcttt tgatttcaga ctgatcaatt
    17461 ccgcaccgtg ttcagcgatt tcggcgatca gctggtcatt ttgaataata acagtcattg
    17521 ccattcctcc aacttcgact tactttttaa aatgggaagg ctggaacaaa catttctatc
    17581 tgccccagtc cccttacttc taataactgt gttttgctaa ctcgtcttta aaacgggaat
    17641 tcaatacttt taaataagtt cccttcatgc ctaacgaacg agattcaatg attccagctg
    17701 attccaattt tcgtaaagca ttgacgatca cagaacgagt gatgccgata ctatcggcaa
    17761 tattagaagc agtcagacga ccttcatctc catccaatgc ttcaaagata gcttgtacag
    17821 ccttcaattc gctataagat aatgtgttga ttgccatttg gacagccgtt gcactccgta
    17881 catctgcttc gatgcttcga gacttctgat aaaggatctg cataccgaca accgtagctc
    17941 catattctgc taagactaga tcgtcatctg caaacgattg ttccatacga gataagataa
    18001 tcgtacccaa acgttcgcca gctccaaaga tcggaacgac agtcgtcaga ccaaatggat
    18061 atttctctct tgactctacc ggaaatgcag taatatcact agtaattggg atattagctt
    18121 cagttttcat caactgtgcc actgtatcag tataagattc aggaaattgt ttctcaacaa
    18181 acattgtttt gattcgttca ttgttcacat cgtgacgttc attataacct agtaatactc
    18241 cgtcacttcc tatgatatag gtgttgctgt caagaatacc acctagtgtg acagccattt
    18301 tatcgtaagg caaatctgct tgcatgtcaa aagtattctt ttgctgcaaa agtgtgttta
    18361 tcttgcgggt tttcgttagc aagtctgtca ttgtcgtcct ccgatttttt ataaaatata
    18421 gcgacttaaa tcttcatttg ccgcaattga gccaagtttg tcatccacat atgcttctgt
    18481 gatcgttatt tctcccattt gcatatctgg tgcttcaaat aatagatctt ctagtagttt
    18541 ttccaaaatg gtatgcagtc tgcgtgcgcc gatattatct gtgtcacggt tgacatcgaa
    18601 agcgatttga gcgatccgtt caattgcttc ttttgtaaac gtaactgaca cattttctgt
    18661 accgatcaac gcgacgtatt gttttactaa agcattattt ggttcggtca gaatcttcac
    18721 aaaatcttca gctgtcaaat catctaattc tacccgtatc gggaagcgtc cttgaagttc
    18781 tggaattaga tcacttggtt tagacaaatg gaaagctcct gaagcaatga ataggacgtg
    18841 atctgtttgg atgatcccgt attttgtgtt tacttgtgaa ccttcgacaa ttggcaatat
    18901 gtctctttgg acgccttctc tggatacttc tccagattgc tgtgatttag aagtgatctt
    18961 atcgatttca tcgataaaaa taattccact ttcttccgct aatttgattg cctcactatg
    19021 gatatcagca tccttgacta attttgctga ttcttccttg atcaataact ctaaagcttc
    19081 tttgactgtt accgtccgtt ccactttttt cttcggtgtc aaagcaccta gagtttcatt
    19141 taggtcgatc cccatttgct ccaatccatt gttcatcatt ggtgcaggtt tcttttggtc
    19201 ttcaacttca atggttactt cacgactatc cagtaaaccc ttttgtagtt gttccaagac
    19261 tgtacggcga ttgattcgaa tatcttctgt cacttcttct tgcggttcag cggtattttg
    19321 ctgactagca ttaaaaagat tcatcatttg ttcaaattga ttagtggatt gtttctgttc
    19381 ttttttgata ccaggtacca agatttttac caaacgtttt tctgcatttt ttttagcact
    19441 tgtgtataca cggctgtact gctctttttc tacgatttgg atcgcatttt ctactaaatc
    19501 ccgaaccatt gattctacat ctcttccgac ataaccgact tcagtaaatt ttgttgcttc
    19561 gactttgaca aatggtgcat tgacgatttt tgccaaacgt cgtgcgattt ctgttttacc
    19621 gacccctgta ggtccgatca tcagcatatt ttttggtgta atatcttgtt gcattttttc
    19681 atctaactgc aaacggcgat agcggttacg caaagcaacg gcaacagatt tttttgctgc
    19741 gtgctgtcct acgatatatt catctaattc acttacgatt tgtcttggtg tttgttttaa
    19801 ttcatacata tctactcacc tcttacagtt cttctacgat aatattatga ttagtaaaga
    19861 tacagatatc tgcggcaata tttaacgcat tttctgcaat ttctttcgca ctcatgtcgt
    19921 tttgagcata gtttttcatt gctcgggctg cagccaacgc aaaattccct cctgatccga
    19981 tagctaagat accatcatct ggtgtgataa cttctcctgt acctgaaaca agaagcattt
    20041 ctttatcatt cattacgatc agcatcgctt caagtttttg catagattgc tgtgtccgcc
    20101 attcttgagc taattcgaca gctgcgcgtt gtagattccc gttatattcg tttagtttgc
    20161 cttcaaattt ttcttctaaa gtaaacgcat ctgcaacact tccggcgaaa cctacaacaa
    20221 cttctccatt gtagatacgt cgtactttct ttgcagtgcc tttcatgaca acagattcac
    20281 ccattgttac ttggccatct ccagccatag caaatttccc gtctttttcg acggcacaga
    20341 tagtggttga atgaaatggt gattcgacca tgtttgtttc ctcctaaatt tgttcttttc
    20401 cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt tcttttgtta
    20461 catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct tggactgtcc
    20521 gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc atgtgaggat
    20581 ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat tcaattccag
    20641 taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct tcgtgatatt
    20701 ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct tgagcaaaag
    20761 aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg acctctgaat
    20821 caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg cctgacccgt
    20881 aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct gctacgctgt
    20941 caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa tttttctttt
    21001 tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga agatactgat
    21061 aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag ctaggacgat
    21121 gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc agcagatcag
    21181 cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca tatgcttctt
    21241 tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt tgttcccacg
    21301 tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc acagcgttta
    21361 taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt cacaattggc
    21421 gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa agagcgagac
    21481 tatccgtctc accctcttca tggagtttcc ttactcattt gggattgcag ttttttctaa
    21541 ttctgttttt attgcctcat tagcttttaa agctcgttgt gcaattgctt catatcttgc
    21601 ttttttgtct cgaatgcgct ctggcagttc aggaaacaga ccatagttgg cattcatcgg
    21661 ttggaaatgt tttccttccg catgagtaat gtagtaagcc atacttccaa tagctgtttc
    21721 ttgtggaaac tcgactggtt ccatttcttt tgctaagcgt gcggcgttga ttccagcaag
    21781 tagtccgctc gctgcacttt caacatagcc ttctacacca gtcatctgac ctgcaaagaa
    21841 cagatcttcg cgtttcgttg attggtaagt cggtttcagc aattctggcg aattcataaa
    21901 gctgtttcgg tgcatcacac catagcgaac gaattcagca ttttctaacc caggaatcat
    21961 ccggaaaaca cgtttttgct cgccccattt caagtgagtc tgaaagccca cgatgttgta
    22021 taaagaagct gcagcattgt cttgacgcag ctggataaca gcataaggac gtttgccagt
    22081 tttaggatct tccaatccaa ctggtttcat aggtccgaaa agcatcgttt tgaatcctcg
    22141 ctgtgccatt acttcaattg gcatacatcc ttcaaaatat ttttctttct caaaagattt
    22201 aagttctgcc acttcggcat tgattagtgc ctcatggaaa gctttgaatt cctcttctgt
    22261 catcggacag ttcaagtaag ctgcttctcc tttgtcatag cgggacttca aatatacttt
    22321 atccatatcg atcgtagctt tgtctacgat tggtgcagct gcatcataga aataaaaacc
    22381 atctgaacca ttgaatgctt tgatgctttc tgctaatgga tgagaagtca aaggtcctgt
    22441 tgctactacg gtgattcctt ccggtatttc agtaatttct tcgtttatca ctgttactaa
    22501 tggatgctct ttgacttttc gggtaattgt ttcagaaaaa gaatcgcggt caacagctaa
    22561 ggcaccacca gctggcactg ctgtttcatc tgccgaagtg attactaccg aattcaatcg
    22621 gcgcatttct tctttcaata accctactgc attagccaaa ctgtttcctc ttaatgaatt
    22681 ggaacatact agttcagcaa aattacttgt gtgatgcgcc tcagtcgatt tgactggacg
    22741 catttcatat aaacggacag ggactcctgc ttgtgcgatc tgccaagcag cttcacttcc
    22801 ggcaagccct gctccaacaa catttaccat tttcaatgaa agaacctctt ttctattatt
    22861 tttggatgtc ttcttcgtaa tctccattta cacagactac ttgtttaccg cctttgactt
    22921 tcttctcgac cagatatccg tcacattttg gacaatttct gcccactggt ttatcccaag
    22981 aagtgaattc acattctggg aaacgggaac aaccatagaa tagtctgttt ttcttagatt
    23041 ttcgttcaat gacttgtcct tcatgacata ctgggcaagt cacaccgatt tcttttacga
    23101 ttggtttcgt attccgacaa tcaggaaaat tactgcaagc ataaaacttc ccatatttcc
    23161 ctaattttat caccatcgga tgtccacaga catcacaatc gaatcctgct ggttcatctt
    23221 tgatttgaat tttttcgatt ttttcttccg cattcgtcag ttctttttca aacggtttat
    23281 agaaacgatc aaccactttg acccacgctt ccgtgccttc ttcaatttta tccaaatctc
    23341 cttccatttc tgctgtgaaa tggatatcga cgatttgagg gaagaattca cagattaatg
    23401 aattgacaat ttctcctagt tcagtcggct caaatctttt ttgtgtcaat tttacatagt
    23461 agcgtctttg gatcgtatca agtgttggtg cataagtgga aggtcgccct accccgtttt
    23521 cttctagtgc acgaatcaac gtagcctcac taaaacgtgc tggtggttga gtaaaatgtt
    23581 gttttggttc aatatcaaca gattggacta catcgccttc ttcaagttct ggaagaatat
    23641 tttctttctc ttctttgccg tcatctctac cttccacata cacttgcatg aatcctttga
    23701 atttgatttt tgaaccgttc gcaatgaaga tgacaccatt ttgttctaat gtcactttca
    23761 ttgtatctaa aatagctggt gtcatctgac tagccacaaa acgtgaccag atcaatgtat
    23821 aaagtttcaa ttgatcttta tctaaatatt gtttgagttc atctggtgta cgaagcacac
    23881 tcgttggacg cacagcttca tgggcatctt gtgcgccttg agcatttttg atctttcggt
    23941 gatcatgtct agaaaattct tttccatacg tatcttcaat aaatgctgcg gcctctgctt
    24001 ttgctgtatc ggcaatccgg gtagaatcag tacgcatgta tgtaatcaag ccgactgttc
    24061 cttgtttacc caaagtgatt ccttcatata attgctgtgc taccatcatg gtttttcttg
    24121 ttcgaaagtt taacttacgg gcagcttctt gttgcaggct acttgtagtg aaaggattcg
    24181 cagggtttct tttgcgctct ttcttttcta cttttttaac ttcgaagtct gttccatcaa
    24241 ttctagcagt gacttctttt acactttgtg catctggtaa tttctttttc ttaccgtcga
    24301 ttccccagaa atttgctttg aatttcttgc gagctttttt aaaattaccg tcgatgctcc
    24361 aatattcttc tggaacaaat tctcggatct ctttttcacg gtcaataata atttttaaag
    24421 cgacagattg gacacgtcct gcacttaacc ctttcttgac cttacgccag agaataggac
    24481 tgatcgaata accaacgatt ctgtctaagg tacgacgagc ttgctgtgca tctactaaat
    24541 ctacatcgat cgttcttggt tccttaaaag ctgctttgac tgcgtctttt gtgatttcat
    24601 tgaagaccac ccgatttttt tctttcagat ccaacccaag taggtacgct aaatgccaag
    24661 caatcgcttc tccttctcta tccgggtcac ttgccaagta aactttttga gctttttttg
    24721 ctgcggcttt caggcttttg atgacatcgc cttttccgcg tatagaaata taatggggtt
    24781 cataattgtt ttcaaaatcg atacccattt tacttttcgg taaatctctt atatggccaa
    24841 cactggccat tactttatag tttcgtccaa gatatttctc aattgtcttg gcttttgcag
    24901 gtgattctac aatcactaaa tatttatacg ccattactga atggactcct ttccattgtc
    24961 gtgttcctac tattaaaaga ggattcatac aaatcgtagc taatcatatc cattcagaaa
    25021 acgtttgtca agaaaagcaa atgaaaagta tggtgttatt ctttaaattc tgccaagata
    25081 tcttcacccg aaaccgtgca aactgcaccg tcttggatca aatgatgaca tccggtagaa
    25141 tgttctgtta aaatatttcc tggaacagaa aaaactgttc ttccaagatc caatgcttgc
    25201 tgtgctgtta tcagagagcc gctttttttc ttggcttcaa agacacatgt tcctttagac
    25261 aaagctgcga taatccgatt tctcattgga aaacaatgct ttttcgggcc ggattgagga
    25321 ggatattcac tcaaaagaag atgatcattt gcaatcacct cttgcaagtc acgattttca
    25381 gcaggataga cttgctctat ccctgttcca atgactgcga ttgtccgtcc accatgagag
    25441 attgctgctt ggtgaccgta tgtatctatc cctttcgcgc agccactgac gatcacatat
    25501 ccagcttcta tcactggttt tagtaaataa taaactgttt ttttggctaa agcagaagtg
    25561 tgtcttccac caacaacgct taacatgtta caagaaagta aagatagatc accattataa
    25621 aacagtacaa ttggcggata ggtcaagtga agcagctgag ggggatattt gggatcaaag
    25681 caagtgataa acgactgttt cgattggatt ttgccccagt tagcgtgaat ttcctgccaa
    25741 cttttgcgaa acagctcccg gtgtttggtt attttaccta tcttaatgat gtcttcaaat
    25801 ccgatttctt tataaggata ctgcatcatc cagttagcaa gtgcccattt tccttgacta
    25861 ctgattcctt ttgtataagc aagtttcaat aaattttctt ctatttgata catcataaaa
    25921 ccgcctttcg tctctctttc taataagata cgtaaaagcg gctgtttttt attgtaaata
    25981 tttttttaca ggtgcaaaac ttttacgatg gattggcgta attccttgtc gttcaagtcc
    26041 ttctaagtgt tcctttgttc cataacctgc attatgcgag aaaccatatc caggataaag
    26101 cacgtcatat tctttcatca attggtctcg atatacttta gcaatgatac ttgcagcagc
    26161 aatcgacaca gatcgtgcat cccctttgat tattttttcc tgctgaatag gcaaatccaa
    26221 ggtcatcgca tcgatcaaca aatagcttgg tacaatggac aatttttcta ctgcttggat
    26281 catcgctaat ttacttgctt cataaatatt cacatcatcg attgtctgct gatcaacgat
    26341 cccgatcccg attgccaggg ctttttcttg aacttgttga taaagaagtt ctcgcttttt
    26401 ctctgataat tgtttggagt cattcagccc taatatgatc tcatttttcg gcaagataac
    26461 tgcagctgcc acgacaggtc cagctaatgg tcctcttcct acttcatcaa tgccggcaat
    26521 ccattggtga ccttgtgcgt acccttgctt ttcaaagcaa aacatttctt catatttttc
    26581 cttaattaat gcttcctttt tcaatcgtcg ttcaaaagaa gccagtgctt gctgcacgcc
    26641 tttgcgtgga tcatttctta atgattctag tgtaggatca tctattctct gtatgctttt
    26701 caatcgttct ttgatacttt ggatggattc agtcatcact tttttgctcc atttcttcgc
    26761 aacgatccag tgtgtatcgt ccaagttttc cttgacgaat atcatgcacg actaattcgc
    26821 tgccacgatc atagtcttct ttatacccac gttttgctgt aatatccatc agtaattccg
    26881 gtgccaattt ttcttcttgc tcgattgata aaccataacg atcgctgatt cttcctgaat
    26941 aatatcgggc aaaaaagtct aatccgtaaa tcgctaaatc atctaaatgg acaagctggt
    27001 ctttgatcgc gcctgtcagc gctagttttt tgccgatttc ttcatcttca aatttgggcc
    27061 ataaaatccc tggtgtgtct aacaattcca actcattacc taaacgaagc cactgctgac
    27121 cttttgttac gccaggctta tttcctgtct gtgcaatttt cttgcctact agtcgattca
    27181 tcaacgtaga tttcccaaca tttggaatac ctaagaccat tgcccgaata gcgcgaggct
    27241 tgatcccccg agcagcatct cttgcccgtt tttctttcaa tgcttcttta gccgcaggta
    27301 ctagaccttt aattccttta ctttcttgtg ccgtgatagc taatgcaaga tggccttgtt
    27361 tacgaaaata ttcttgccac agctttgtct gttgtggatc tgccagatcc gctttattca
    27421 ataaaacaag gcgaggtttt tgctgaatga tctgatctag cattggatta cgagaagata
    27481 aaggtaatcg tgcgtctacc aattcaaata cgatgtccac atatttcatt ttttcagaaa
    27541 cttctcgtcg ggcttttgcc atatgtcccg gaaaccattg gatggtcatt gttctctcca
    27601 ttcctttcat accggaatat tccccggctt tacttgtttt tttctttttc aatataaatt
    27661 cactttttta ttataaagaa attccatcta ttagttaagt gaaagtgaac cagtcctcat
    27721 tctatcacat cttcactttt ttgatgatgc aaatttttat tttggtttct atatatgaat
    27781 ctaaattttt gacagcgttt cctaaataag agatgataga cgaacaaaac agaaaggaga
    27841 cgaaacaaat gaatagtatg gtttttgtta attttcctgt tgctgatgtc aatcgttccg
    27901 ttgcttttta tgaaaagctg ggttttaaaa aaaatgaaga attttctact gaagaagctg
    27961 gttcaatggt atgggatgac actttttgga taatgcttct ctctcatgat ttttataaaa
    28021 aatttataaa agataaagaa atagctgata ataaaaaaac aagtggtgct ttgatttcgt
    28081 ttagtgttga aaatgcggat gaagtaaaaa aatttgctca aattgcaaaa gaaaatggtg
    28141 gtactttcca cgaagtagat atgggaatgc ctgaagaaga aatgttttca ttagaagtcc
    28201 aagatctaga cggaaataca ttagaacccg tatggatgaa gatgtaagca gataactaat
    28261 ctgacgtttt ttataacaaa aaaacaaagg cggatgatac gataagtatt ctccgcctct
    28321 atttttttta ttcgtacaat ctctttttta cagtttataa aagcagtctt ctcacccttt
    28381 ttttaattgt tagatgccag tgtatgtaaa tcaccaatgt gtcatgacac attggtgatt
    28441 ttatggtatc cttttattat taactgacaa aggagagaat attttgaaca ctttgattgc
    28501 aaaagacgtt tttagattgc ccgaaatgaa tgcaagattg ttgtcgacct ctgctctttt
    28561 attagccctt gatgtcatct tatacaagat agcgattggt cctgcttatt ttcaaatcac
    28621 attcggattt ttaagtatgg cgcttatggg ttatctatat ggaccgatct gggctggttt
    28681 gcttgaaatg ttgtcgaaca ctttaaattt cacgatattt ggttcaggaa cgtttcaagc
    28741 tgcttttctt atcacggctt tccttggtgg atttattaat ggcttatttt tatatcataa
    28801 aaagataaac ctgccttccg tcatcgctgc ccaattattg attatgatcc cagtcagcct
    28861 ttttatcaat tcttggctga tgtctgtttt gtttggtgca gatttcaaag taatttttct
    28921 aggtagattc atcaggaata tgatccaaat ccctattcaa ataatggttg tttattcatt
    28981 tatcaaaact ttagaggcta gaaaagtgtt taaacgagct gtttgatttt acgtactaag
    29041 gtagtaaact gattttggat taaacaataa agcgtgcagt ctcaacagac tgcacgcttt
    29101 attgtttctc actggatcca ttttatttca tcaagtggat aatagacaaa tcttgcttta
    29161 cctagaatat cagttttatt gatcgtgcca aacgaacgac tatcttttga aactcttcga
    29221 ttatctccaa gtacaaaata attatcttcc ggaagcttgg attgtcccgt tagctcttgt
    29281 agtgtaaagt ccgtcgtata ggatgcattt gcatgatctg atttcaggtt tttagttaga
    29341 taaggttcct taactacttt cccattgatc ttcaattgat cattttggta gctgacattt
    29401 tctcctggaa gaccgattac tcgtttgata taaatcgtgc catctgctaa ttgaaaaaca
    29461 acaatatcaa atcgtctgat ctccgaaaat ttttccatca caaccatatc accttgcttc
    29521 aacacgtttt ccattgaatt gccttccact ggtaccggga tgaataaaaa tccccgcaac
    29581 atgaacgcaa ggaatacaga aacaagtaag tatttgaata caagccagaa gcgattaata
    29641 tagcgttgtt tttttgtcaa tctctatcaa tccatccctc tttttttcat tctttcttta
    29701 ttataagaaa aatatcaaaa aaatgcgact agatagctga aataaaaata attctttcaa
    29761 ttaggctatt ggacaaaaaa cttttttgat cataaagtac aaaactatct cttatatgtc
    29821 atgattattt tctaaatatt tttcccatgc ttcgtcaaag atatccatgg acggcaaata
    29881 gtcgacattc agttccagat aattagaaat ttcttcgtag ctagagctgt gcttaggaaa
    29941 ttggatgtct ttaccggctt ctgtagcgaa aacagataaa gcatcatgtg tgccaccgcc
    30001 tcgcagtgtc attaaataat gaaaaaagct tcgattcacc cttcgttcct ccaattttct
    30061 tcgataaagg cagcccggcg tgcatggttt cttttaaaat tttcaggatg ctttttataa
    30121 tactcttgat ggtcttcttc tgctagataa aatggttggg ctggttcgat ttttgtaacg
    30181 attggctgtt cgaaacgtcc actttcagct aactgtttct tacttttttc agcaatgatc
    30241 cgctgttctt ctgtcaaata aaagatcacc gggcgataat tgtcaccgcg atcctcgaat
    30301 tgtccgaaag cgtcagttgg atcagtttgc tgccagtaaa gttctactaa ttgttcatat
    30361 gaaatcaatg ttggatcata gataatctcc actgcttctg tatgtccagt cgtttggctt
    30421 tttacttgtt cataagtcgg atctggtaca tgccctccag tatatcctga catcactgta
    30481 tggataccag gaagcgtgtc gaaaggctgg atcatacacc agaagcagcc tcctgcaaat
    30541 attgctcgtt ccattatgat tccccctcta atactactca tccgatgatt ttttattttc
    30601 ttttgtcttg ctttcagtac tgctggtgct agttttgttg gtatttgagt tgtttgaagc
    30661 atcagaggta tcttttggca aatatagact aaaccgaatg tcatcattta ctaagtcgat
    30721 tttatctgct ttaatataca tcccgttttg cattcggaat tgatccaacc taagcatgat
    30781 agtcagatca tctgtattga cttcgatcca atctggtaat ttatagtttc tcttgatcat
    30841 attcatcaca tctttgattg gcaaactcaa tgttccgata gaaagacttc tagcttttag
    30901 ttggacattt ccgttttcca tcacatacgg atcaaaatat aaatagaaat cgataggaaa
    30961 acctagtact tcgaaagtac cattgagtag tgcttcattt tccaaataaa atttatattc
    31021 aatatcacta tctttttgat actcactaag gaaaaagctg atgatttgat tgactttttc
    31081 tttatttgaa ttgatcgtta agacaggatc tccttgtctt tccgtaagag ctggtatttc
    31141 tgggagttct ggttcccgat tagcaaaaat acggctgcct aaaaatacgg tcgaaccaag
    31201 tactacccca agaaggataa ggaaggcgaa tttccagtaa tttgttttct ttgttttgtt
    31261 tgaagatacg tcagatcttg ttttcatgtt tattgatcat cctccttctc taaccattgg
    31321 ttttgtgtct cgatcagctg atctctcact gcatttgcca tcaattgata acctaaatta
    31381 tttggatgga aatgatcttc ttcatagagc acattatttt tgatgccatt tagattttct
    31441 gtcgtgctag ttgtttgatc tgttccttct tctgtgacac ctacttcctg attcaatcct
    31501 tgataaagca aatcattgat tgggataaag tagcagttcg gcattttttt tgtcaccgtt
    31561 tctgttcctt tgttccaatt gttaacaatt gtctgcatgt cagtgatctc aggaaagttc
    31621 aaataaaatg gattgtagat gcctaaaatg taaataggtg cggttttatt gagggatcga
    31681 aattcttcca atagttctgt gacattttcc tgatagtttt tcagtggctt tttaaaggaa
    31741 ttgaccgtta acccgaataa attattttgg ataactttca tcaaatcatt accgccaaca
    31801 gtcagtgtga taaaatcagc tgttttgata ttgtttcgaa tttcttcttt tgtttttact
    31861 cgctttaaga tctggtcact tcgttcacca tttacaccat agttttctgt ttcaaccgcg
    31921 gtcaaattat aacggtcttt tagatcattt gcgacaaggg gaacgaatcc tccttgcttg
    31981 gtttgatctc ctactccttc agtcagcgaa tctccgatag ctatgtagtg aagtttttct
    32041 tttttatttg gatcttctct cacagtagtt actggtttca ttagtgggcc agctttcggt
    32101 atagaagcag ctaaacctgc atagatgatg atcgcaatta ataatgggaa caaaacgagg
    32161 atcaaatgtc gtttcgttaa tttcattttt tcacctcttg tattcgattg gttctaatgt
    32221 tatcaaaaat tttatcaaaa aggtagtctt tatgaaaaaa gaaaacaatt attttcctaa
    32281 ttttataaag aaaaagagaa taagctgcaa gcctttcagg ctgagggctt atcctctttt
    32341 cgactaatct acataataca tgattgcaaa cgcgtcttta ccagtatgcg tagcgataac
    32401 cggattcgta tgcagtacag ggatatgcat gtccgggaat aattcttgca aaccctcttt
    32461 gaattcttgc gccaattctt ttgcatccgc atgtgaaata ccgatttgtc gcacatttgg
    32521 caattctttc agttcatttt tcaaattatt aaaccattta tcgaacgttt tctttcctcg
    32581 ccccttagtt acagggatta attcagaatg ggcgaaattc atcaccacgc gtatgttcaa
    32641 aacactagac agcaaaccag ttgttcggct gattcttcca ccttttacca agttatctag
    32701 tgtggatatt ccgatgtata attttgtatt gtcttgtact tttttgactt cttcaaggat
    32761 tttctctact gttttacctt cttttgctaa ttcagctgct cgaattacct gaaaagataa
    32821 tccttggtca gtggtatcac tgtcgataac cgttactttc gtttttgtta gattactggc
    32881 ttgtcttgct gcttctactg ttccactcaa gccttttgtc atatggatcg aaatcacttc
    32941 actgccgtct tctcctagct catcatacat cgagatgaat tctccaattg gtggttggct
    33001 tgttttgggc agattttttg ctttcgccat catttccata aatctttcgc cgtctagttg
    33061 gtcatcatcg ggatatactg ttccgtcgat catgatggat agcggcatga cacgaatgcc
    33121 taaagcatcg cgtacatttt tttccatcgt acatgaagaa tctgttacga tttttacatt
    33181 tgtcattatt ttctctctct ctttctttaa caagatgcct tattcatgtt aaactacatg
    33241 taagacaatt tctattttta gtataacaga caaataatga tttttcatta attacaccta
    33301 gtaaaggagc cgttttcatg gaaaaaacga aatttaatcg aacgtatgaa atattaaatg
    33361 aagtgtttaa tgcagtcacc catggaattg ggactggatt gagtatcgcg ggactagtgg
    33421 ttcttttagt aaaaggtgcc cacttgcatt cctccctcca tattgtctct tatgctatct
    33481 atggatcgat gatgattctt ttatttttaa cgtctacttt gtttcattca ttgattttta
    33541 caaaagcaaa aaaggtgttc caagtattcg atcatagttc gatttttctt ttgattgcag
    33601 gaagttatac tcctttttgt ttgctgagta tccgaggatg gctaggttgg actttatttg
    33661 tattaatctg gttgtttgcg attcttggca ttatatataa agcgttgacg ttgcataaac
    33721 gggagagagt atcaaaagta tctacgatca tctatatttt tatggggtgg ctctgtgtcg
    33781 tggctgctgt tcctctttat cactctcttg gaatgactgg tgttattttg atggtagcag
    33841 gcgggctttc ttacacgata ggcgcattct tttatagctt gaattctatc cgctatatgc
    33901 atgttgtttg gcatttgttt gttatgttag gtgcaggttt tatgtacttc tctgttttat
    33961 tctttactta ataaaaaaaa taaagctatg gatgggacag ctctaaataa cagctagtct
    34021 atccatagct tttttatatc gtcaattcgc ctgtcccgct ttttacagga ttctcattac
    34081 gctgatacgt ttcaaattga tgcggataac gatttttttc atccatttct ccttgactag
    34141 tattgatcag tgaccaatca gaccagtcaa caggtggaaa ataagcatct ccatcgaata
    34201 catgatggat gactgtacgg tataatatcg tacaaaatgg cagaaattct tgataaacag
    34261 ctgaccctcc agcgatgaaa aagataccgt ctgtttcttt ttccagagca agcacgtctt
    34321 ccacactatg catgacatgc acgccttctg ctttatagga agaatctctc gtcaaaacaa
    34381 tcgtctgacg attcggcaac ggacggctac ccatcccttc gaatgttttt cgtcccatca
    34441 ctagtgtatt tgcttctgtc atttgtttga agaatttcag atcatttggc aaacgccaag
    34501 gaagacggtc ttcttttcca atcaatccgt tttcatcctg tgcccaaatt gcagctaaca
    34561 ttttctcatc ctttctgatt ctaaacagcg ataggagctt tgatcgccgg atgtgggtca
    34621 tatccttcta attggatatc ttccatctca aaatcaaata ctgactgctt ttcctgattc
    34681 aaaactaaag tcggaaatgc gcgtatttct cggctcaatt gttctttcat ttgttcgata
    34741 tgattgatat aaagatgagc atctcctaat gtgtgaacaa attcgccaac cgctaaacct
    34801 gtttcatgtg caattaggtg agtcagcaac gcataacttg caatgttgaa cggaacgcct
    34861 agaaacacat ccccactgcg ttgatacaat tggcagctta gccgtccctc ttgaacataa
    34921 aattgaaaca tcgtatgaca aggaggcaaa gccatgctag gaacatcttc cggattccat
    34981 gcagaaacga tcaatcttct agaatcagga gtctttttga ttgcttcgat cacttgtttg
    35041 atttgatcga taaagccacc gtcttttgtt tcccagtgac gccattgata gccgtagatg
    35101 tgtccaagat cgccgtatgt ttcagcaaag gaatcatcgg ttaggatctt ctcgcaaaaa
    35161 agcttgctct gctttttata ttcttcattg aattcaggat ctgtcaaaca tctatggcca
    35221 aaatcagtca tatctggtcc attatagtct ggacttttga tgtagcgttc aaatgcccat
    35281 tcatcccaaa tatggttttt attttgtaat agatagcgga tattcgtatc tccttttaaa
    35341 aaccaaagca attcgctttt gatcaatcca aaaggtactc gtttcgttgt taacaaagga
    35401 aacccttttt gcagatcaaa ccgcatttgg tatccgaata agcttaatgt gcctgtgcct
    35461 gttctgtctt ctttacgatg cccttcttcc aacaattttc ttcctaagtc aagatacgct
    35521 tgttccatcc tttttcctcc taatcttcta cccattcact caagtattcc caccgtgcca
    35581 ttttttcttc aagttgtgct tctgtttcag atacgtcgtt ttgcaattct tgtaatcgag
    35641 tgaaattatc tccttgatga ttcatctctt cttggagcaa tgagattttt tcttcaagtt
    35701 ctgcaatctc atcttcaatc gtttcccatt ctttttgttc catgtaagat aatttcttct
    35761 tttcagcagg ttccttgctc tgtatttttg gtttcgcttt ttcagcaggc tgctgggtct
    35821 ctttttgctt cagtaaataa ctggacatgt ttccgtaata aagttcttgt tttccgtttc
    35881 cttggaatac taataactta tctgctacct tgtccaagaa ataacggtca tgggaaacag
    35941 tgatcacagc acctggaaaa cttctaaaat aatcttccaa aatcgtcaat gttgctatgt
    36001 ccaaatcatt ggtcggttca tccaataata aaacattagg ttgctgaatc agcagtttca
    36061 acaaaaataa ccggcgtttt tctccgcctg aaagtttgcc tatcagtgtc ccatgcataa
    36121 atcttggaaa taaaaatcgt tcaagtaatt ctgctactcc gatttgactg ccatctgctg
    36181 tttttgcttc ttcagcagct tcttgtaagt aagcaatcat ccgtttatct ggtgccattt
    36241 cttcattttc ttgtgtataa tacgcaagat gaaccgtttc accgatagac attgtaccgc
    36301 tatccagggg aattctgcca gctagaatat taagcaatgt cgattttccc gcaccatttt
    36361 taccagtaat acctagtctt tcccgagatt gaatcaataa atctaaatgt tctagcagtg
    36421 tttggtcatt tatacggtaa ctgccatctt tgatttccag tacttttttc cctaaacgtt
    36481 gtgtcgctag gttcagctct aaatcatctt cttggtttac ttggtacaag ttctccttta
    36541 gatcttcgaa acggttgatc cgtgcttgct gcttggtgcc acgtgcctgt acgccagccc
    36601 tcatccaagc taattcctgt ttataaagcc gcttgcgctt ttcttcttgt tctacttcta
    36661 cacgatcacg ttctgctttt tctaagacgt aagcttcata atttccttta tactcgtata
    36721 attttccaaa tgaaagttcg aagattcggt ttgctactct atctagaaaa taacgatcgt
    36781 gggtaaccat caatagtgcg ccacggtatt gtttcaaata gttttccaac cactcgatcg
    36841 cttcataatc taaatggttt gttggttcgt ctagtaatag taaatcgggt tcatcaatca
    36901 acacttgcgc cagactaact cgttttttct gaccacctga taaagtactg atttttttat
    36961 ctaattcact gattcctaat ttttgcaaaa taatctttgc gttggtatct gtagtccaag
    37021 catcttcttt attcatttgt tcttctgctt tagcataacg tttttgcaca tcagggtctt
    37081 ctccgttttc ccctagttcg atcaatgctt cctcatatgc tcgaacagtt tggatcaagg
    37141 ggctgtttcc ttgaaagact gcttggagaa cagttaattc atcggaaaat tcagctgctt
    37201 gagaaagata accaatacgg taatcagttg gataaaacac tgtttgccga tcaccgtcgc
    37261 tgctatcgat accggcaata atatttaaga gacttgtttt acccgtcccg ttcgtcccaa
    37321 tcaacccgat acggtctttt tcgtggacca aaaaagaaat ctggtcaaac aaatcttttt
    37381 caccataagt ttttttgagg tcagttactt ttaattcttt caacatgttc cctccttatc
    37441 ttggatattg tatcataaaa atagtgaact tcaggaaaat ttttttggta tttgttcttt
    37501 ttgtgttctc aaaaaaagaa caggaccgat tcgatcctgt tctgatatgt tttgtttttt
    37561 ataaaacttg aaaattttta cctttttatt ttattggact cgtttttttt gactttgctc
    37621 gaaacgcaaa tcggaaagaa aagttatcag atggtttgtt catcgtataa gattctaaca
    37681 aattgatatt cctgcgatag ctgttcgcag aaaacgtcgt gattttttca gcaacttcgt
    37741 attcatcaat gatttttctt tcaagataat aacctcttag catttttttc gtaaacagac
    37801 tatcttgctg gatcatccac gaagaaatca aattccccga gttcattttt attccttcaa
    37861 gtttgcgttg ctggataaaa aattgaacca tcgtttcatc ataaatatca gtcaagttat
    37921 ctaaactttc aataatcaac tgagtatttt tttgatatgt ctctctgatt tcttggatat
    37981 cttcatcact aataaatgtc tgacgatcag catatcttct ttgccaaaac attttcggat
    38041 ggagaacgat tcgcattaac ctacggacaa caatgaacca aaagccgata aaagacaaaa
    38101 tctggcttgt gatagaatgt tcgaatcgag aaagaaaacg ggagtaaaaa cgataaccat
    38161 tagaagaaag cttgcccgaa cgataacttt catccaatcc atcccgttcg atcgacaaaa
    38221 tcagtgcttg tatttcttgt acttcttgct tttcatcatc ggttaaatct tccaagtaca
    38281 gatcacgtat ccgttcttga taattttcta tcactgcttc agtggctaaa aattcttttg
    38341 tattacgatc aatttccttt atttcttttc gcaaaacttc aacgacctct tctaaaatca
    38401 tcaaagcatt caaatccgtg cttttcgctt cctctgtttc cgtcagcata ggcaagacaa
    38461 tgatccctat gaccaaagtc actaagatga cacccgccgt cagaaacaaa agcaaagacc
    38521 gctggtaaaa catcatatta ttgattgaaa atggcaaaat aaaaatcgta gccaaactga
    38581 ctgttccctt tacaccgcca aatgttaaga tcaagatctc gttcaactgt tttttgaact
    38641 ttttactgcc atctttgaat acataaaaaa gactgataga aatgaatctg atcacaaaaa
    38701 gcatgacaga gattaatacg ataatcgcta ataaaagacc gtttggatat aaaccgtctc
    38761 cccatactgg cgagaacact tgcgtcaatt cgattcctaa aaatataaat actaatgcat
    38821 tcagtgtaaa agcaatagtt gtccatgtac tgtgggacaa gctcgacaat tctgcatcaa
    38881 acacagagat tttcctaaaa ccagaagctt ggagaatacc tgctgccact gcagcaatga
    38941 ttccagatac atcaaatgcc tctgccaata cataggctag aaaaggaagc aaaagttcga
    39001 tcagcagata ccctgtgaca tcttgtgctg atgctttttc gatcaaatga attatttttt
    39061 gtttgaacca gacaagcaaa aagccgatca aagctccccc aatactagaa actactagac
    39121 tcatccccgc gtttactgct gaaaaagatc cggtgatcag cgcaccaaga gcaaactgga
    39181 aggcagtaac accagatgca tcattcaaaa gaccttctcc ttccaaaata tgcatcgcct
    39241 ttttagggat attcactcgt ccagaaagag aactgactgc taccgcatct gtcggaccta
    39301 acgctgctcc aaaagcaaag caggcagcaa gtgggacact tggcaacagg aatgataacg
    39361 tcgcacctac cccgacaagg gtcaaaatga ctcctccaaa tgccaagaac aaaattgtcc
    39421 cgaaattttt gagaatcgaa gaaatatctg ctttttctcc ttctctaaat aacaatggtg
    39481 caataatcat aactaagaac aattcaggtt caaaatcgat tgattgtccc cactctgtca
    39541 gacctaatac cactccaagg aaaatttgga tcagtggagc tggaatcatt ggcaaaatgc
    39601 ggctagctat gttcgataag gtgatcgtta ctgcaaatac aataactaac tcagcaaatt
    39661 ccatcgtcca tcccctttat tttttatttt gtgtaagcat ttttaaaaat tgttcttttt
    39721 tctttaaaat tttggtttcg actacttcaa cctgtgtttg gttcccctct aattcacact
    39781 gtacacgttt tgactccaaa cgaagcagat catgtttcag acgttccaac tgacgataca
    39841 tcaatatatt tcttttacgc aacttacaaa gtatttgacg gcggtcatcc atcgaaaggg
    39901 cgccatagcg atcatgcaac agtttttttt gttgttcaaa actctctctc attttccaga
    39961 tctcctatac acaaacttcc atcattatag caaagttctt tttggatgaa aaatatttca
    40021 ttgtttcatc gtttattgct agttagaaat ttccataaat ttttcaacgc ctcattctat
    40081 tccaaatagt atcagatatc aagtaaaact taagaaagat aaaagtttct gacaaaagga
    40141 tgtttacttt cccattcctg cctttcttgt tcttttatca caacaaaaaa atggggctgc
    40201 gacaaaaatc ttgtcacagt cccttattct gaataaacgg tgtggaagag acagcctatc
    40261 agtagtaata agacacatct ctttcacagc ctcatttttg cgtaaaaatc gatttattcg
    40321 aaaaattccc cgtatacatc ttctacacgt tgatctggat tgatcacgat atacaagtta
    40381 cctgtttttt cgctgatttt tgttgattga tattgattgt ctaatccttc tgttccttta
    40441 tctagatatg ggaagtacac aagatcaggt gtgccatttc ggtaaaggat ttccattcgc
    40501 tcgatatctt cgtatttcaa tgcgcgatta aacatgccca tttccagtcc gcctaggttg
    40561 atatcatgtg tcgcaactgc gtccccttca tggaagattt cgattttaaa tccagcacac
    40621 ggatggattt caacaaattc actgccattg attcgtccaa aacttgttgt gatttgtttg
    40681 atccaaagat cgccgatatg acggcggttg atcgtccagg tttctccatt tctaaaataa
    40741 aattttagcg ctgtcatttc tttactcatt aaaaagactc ctttatgatg taatttattc
    40801 ttgtactacc tcagtttagc acaaatttat gtaagtggtc acatcttatg ttttttcttc
    40861 taatgacgta tctgcccact ttaagtagcc aacaaagctg agcagataca gtctagtgtt
    40921 ttttaaatca atttttcttt cgataaaaaa tccaaaacct gttttttatc attttttatt
    40981 ctttgttcta cgacggcttt ttcagctaat tgcaaagctt cactaaccca cttaccaggt
    41041 ttttttccag tttcttctag caagatttta ccatctactg ctaattcgct taatgacttg
    41101 ataggtaaac gatcatagga ttctcctacg gcatccatct gaggtgtttc gtcaaaatag
    41161 atcatcaatt cttctacttg cagtgcatgt tttttgccta agaagtacaa ttcgaatggt
    41221 tccaaagctc cagtttctcg ttgtcgtagt ccttggacaa gtgcttgaac gtctttaatc
    41281 atttggttag aacacttcca cgatttcaaa aaggaacgaa tctcattttc ttttagatcc
    41341 agttggctga tcaataatgc ccatgcttgg ctctcttctt tcaatgggac ggatggtaat
    41401 tcagcaaaac gtaaaagtgc ttcgccttct tctttcaaac cgggacagca aatgtagcac
    41461 tctgtttcaa taaatgcatg gattcccgcg gcccgttcat ttcccaagag catcttgatg
    41521 aattccacat ttatacgttc tacagatatt ttacctaata gctcgtggtt ttctttgatg
    41581 gcgtagaagg tttcttcctc taatttgaac cctagttgac tgacaaaacg caacccgcgc
    41641 atcattcgta aggcatcttc atggaaccgc tcatgaggat tgcctacagc tcgcaagatc
    41701 tgttcttcta gatcttgcag accatcgaac aaatcgatga tttctccatc ttctttcaaa
    41761 gcaaacgcat tgatcgtaaa atctcttctt ttcaaatctt ctgccaacga acgaacgaat
    41821 tcgacatgat ctggtctacg gtagtcctga taagttgatt ccgtccgaaa tgtggtgatc
    41881 tcatattgtt catcattttc taataccaac acagttccat gttcgatccc aatatctatc
    41941 gtttttggaa aaagctgttt gatttcttct ggaaatgcgc ttgttgcgat atccacatca
    42001 tggattggct ggttcagcaa gatatcacga acacttccac ctacaaaata agcttcatat
    42061 cctgcattct ctaatttttt cagaactgga attgcctttt ggtattccag aggtaattgt
    42121 tttaatttca tttttccaac ctttcctgcg tgtttcttgt cctgagataa agactcagtt
    42181 gcatggtgac tgtttgacaa gtccgtttca gagtcttcaa ggccaggacc acttcatctt
    42241 gtttactcgt ccttacgctc aaagcgatta tggtctcttt gattgaattt ttccatgttt
    42301 ttttcaaaaa cttcagttaa gtcgatattc aatgaattag ccatgatcat cgtaacgaat
    42361 aaaacatcac caagttcttc tgctaccgag ttaactggtt cagaagcctt tttgcttttt
    42421 tctccataag tgtggttgac ttctcttgcc aattcaccta cttcttcagt taatcgagcc
    42481 atctgagcta atggagagaa ataccccacc ttgaattgct ggatatattc atccacttcc
    42541 tgctgcatac tgtataaaga acgcttttta tccatctttt tccctccaaa actatcttac
    42601 caaaacttta acgcaaatcc tagataagag caattgagaa aatcgaattt ccatgctaca
    42661 tttaggaagg aatttctaaa tggaaattct gtgaaaatac ataattgaaa gctggaattc
    42721 atatggtcaa acgaaaaaca gtaaaagaag ttctttttat cattatagga acaagtattt
    42781 atgcatttgg tcttgtttac ttgaacatcg ctaatcattt agcagaaggg ggagtctcag
    42841 ggatcacctt gatcttaaga gctttatttg gtattgatcc agcttatacg actttattaa
    42901 tcaacatccc attgatttta ctaggaggaa agatcctcgg taaacgttcc ttggcctata
    42961 ctgttctagg tacactttct ttgtcattct ttttatggat ctggcaaagg gttccactag
    43021 aaatcaattt acagcatgat ctactgatcg tttctctact tgcgggttta gttgcaggtg
    43081 taggaagcgg gatcgtctac cgcatgggtg gaacaacagg cggcagtgat attatcgctc
    43141 ggattttaga acaaaattac ggcatcacta tggggcgttc cctattggca tttgatatca
    43201 tcgtcttact tgcttcatta acctatatcg acttgaaacg tatgatgtat acgttgattg
    43261 tttcgtatgt tttcagtcgt gtgattgatt cgatcttaga tggcggatat tctgcaaaag
    43321 gtctacttgt cgtctccaac aaaagcgaag agattgcacc actactgatg accggtctgc
    43381 aaagaggtgt gactttcctt tctggagaag gcgcattctc tggtaattca aaaaaaatca
    43441 tctatatggt cgttagctct agtgaattaa ctgaagctaa gaggatcgtc cacgagattg
    43501 acgaaaaagc tttcctttca atcatcaatg tgcatgaagt ggaagggcaa ggctttactt
    43561 atttgaagcc acagacaaga ttactgaaaa aactataaga aaaaagacaa agataggcta
    43621 tgaggaaaga ttccacatcc agctgacctt tgtctttttt agtactatac tatttttaac
    43681 taattgttat gttcacttac ccggttactt ttcttttgtg tctgcaaaag gatctttcgg
    43741 ttaacttcat cagataattt caaaaaagca tccctatctc catcttctag tgcttgatcg
    43801 atttgagcat aaagcatttt tagttttgcc tcttcttctt tttcttgaaa atatcgttca
    43861 attaattcag tatcttcttc actgactgaa gaatcccaag aagaaaatgg attgtcttct
    43921 aagatagata gatattctct tgtctgccaa gcattttcga atatacattc cacatataaa
    43981 ggctttttcc aattcaaacg gatttcatga aaaatctggt ctgtgtccgt aaattctttt
    44041 ccctgcaaaa acaatttcat gggttcccct tcaacagata gatcactgat ctgcagtcct
    44101 cgatccgcaa aattcgcccc ttcaacaaag tggacattgc ttaaaatcgc ttcatgattg
    44161 gcaagataat tcaagatcca aaaaacttct cttcggctaa aagatacatg attgaccatc
    44221 cacgttaaaa aattcttctt ttcccgtaca tcaattagca tttttctcac ctctctgtta
    44281 ggtcatctaa taatgacagt gcctccatat ctcctggttc aagtgttaaa taataagtca
    44341 ataattcttg cgctcttttc agctgccctt cttctcttaa gaaaaaggca tactctttca
    44401 agaaatctgg ttcatgtgat agttcatgat aagcctgttc atagtgtaca gctgcaacgg
    44461 taaagtcttc taattcatta taagcatggg ctaggttcca ttctgcataa ggattagcag
    44521 tttcttccat ctgattgatc acttgtatca catcttcata tctttcatca tccaagtaaa
    44581 gattacttaa agtaagtaat gtttcatctt gtttgtctcc tacttctaaa gctttcagta
    44641 aaagttcttc tgctcgctgt ctatcatgca gacggaaagc attttccgag gccaggtgat
    44701 aaagttcgac ttggtaagga ttttctttga ttccttcttc taacactgtt tgtgcttctt
    44761 caatcatttc ttcttcttga agagcttgtc ctagatatag atataacgat tgataatgcg
    44821 gattaacttc tcgaagttct tccagatagt tgattgcctg ctgattttct ttcaattgaa
    44881 gattcgtaaa agctagctgg aacagtcgat catctgtcct ttcttctttc agtgcttctt
    44941 ctaaaggtac gatagctgct tcgaattctc cttgcatact taaggattgt cctaaacgtt
    45001 cctgcatcga gattcctgaa atttcattta tcccgcttgc caacaatgtt tcataaacca
    45061 ctgctgcctc tttgaaacga tcaactgaaa aatacaattc agctagcgca aactggatca
    45121 atggttcatc aggaagcaac ccagctgctt ctttcaattt tgcttcactc acttcaggga
    45181 ttccgataac ttggtacaaa tcagctatcg ccaataagct ttgcggatag taatcacttt
    45241 gcttatccac tttttccagg tattcaaaag ctaagtcgat ctcattatct tcgattgcaa
    45301 tttctgctaa cgggatgttc agtccgtcat tttctgggta ttggttgatc agttgttcaa
    45361 aaatctgttt tgcttcgatc aaaaagccaa tagccagcaa ttcttcgcct agttcagcta
    45421 atatatctgc tgggtcttct tttaatgctt tttctaaaaa gatttgtgct tgtgacaagt
    45481 cttcatctga taaagcttgc aacatttttt cgctatttgt attcatttca attccctctt
    45541 atctcactga cgatttgttg aaataccgtc attgttctca ataattcttt ttcgtttgga
    45601 tgttggtatc cgtctattat acctattttt tctaaaacaa gtattttttt gcttttttct
    45661 gccaaaaaaa gaactttttc caaatactcc atctttgaaa tctgttccag ccagtggatt
    45721 ggatagccaa gcaagtccag ccacacaaaa aagtttttta gttggaaatc aaaaggatat
    45781 gcttggagat tccacacgaa atgaaacatc attcctaaaa attttttcat actagcagaa
    45841 agtagatggc cattttcggt cagataaaaa gcttgttcaa acagtttacc ataatttgac
    45901 aggttttctg cctctaattg ataaaaatag atcatttctt cgatcatccc ggcaaacggc
    45961 cgagtcgtta attgcttttt atttggatag tttttataca gattttgcaa aaaacgatga
    46021 tcacaaatca ttccgcaaca gataaaagtc aaaaagtcaa tctgctttcc gtccacctgc
    46081 cgctcgctga ttgtttgatc ataaacgatg cgttctggca gattgatggt ttgcagcact
    46141 acacgatttg gtagctgcaa aatcgtccgc tgcggtgtta atgctcggct tagagaacgg
    46201 atcgatacag ggatcatcca tagtgatgaa tcaagggttg tatgtttttg aaaaaaacct
    46261 gccagatcca tgatcccttc atttccaaat ccgatgataa acatggattg attttcggga
    46321 tagcgtttga cgaaatcaag caggttcttt aattctgtta aatgattgca ttgagtgttg
    46381 gcacaaatat accagtcatt gtttgcttga tttgcaaata acgggttgat cttatctgca
    46441 aacaaatcat aatagcgctg attcgttaaa aataagattg gctgatcaat ggaaagttct
    46501 cttagctgac tgactagcgt ttctccgtaa aacactttcg tcaacgcatc ttctacttga
    46561 taatcaagtt ccatacgcat ccctcctctt cttattttta ttttaccatt agtaagcaaa
    46621 ttctagaaaa gataagagca aagctcacct cataacttgc aaataaataa aaattaaata
    46681 aaatacatgt gttttctatg tatgacatac ggattcggaa tctctgtctc ttatttattc
    46741 cttttctgct aggaaataaa aagaaaagca cttgtagccc tagagccaca agcgcttttt
    46801 caactgtctt atttaacagc gtcttttaat gctttacctg gtttgaatgc aggtactttg
    46861 ctagctggaa tttcgatttc ttcaccagtt tgtgggttac gacctttgcg agctgcgcgt
    46921 tcgcgaactt caaagttacc aaaaccgatt aattgaactt tttcgccgtt agctaaagca
    46981 tcttgaatag ttgaaaatac agcatctact gctgcagttg catctttttt agttaaatca
    47041 gtagctgaag caactttttc gattaattct gctttgtttg ccatggatag attcacctcc
    47101 tccacaagag atatgataga aatatcattt agttaaaact ttctgagtgg tccagaagtt
    47161 taaaaagaaa tattgattaa cttcaatatt ctgttatcac gatatcatat gaaccgctta
    47221 atagcaaggt ttttaccaat tttcacatgc tttttttagt catagaaacg taacaaacga
    47281 aactgtcttt ttcttttgaa aaaaaaacgt cgatatggta aaatgcctat tttctcctac
    47341 gagggataat tcggatggac gtcccttcaa aagtaaatgc ttttctgatt tgattttcta
    47401 agaaacgttc ataagaaaaa tgcatcaatt cctcttcatt gacaaaaacg acaaatgtag
    47461 gtggtttgac agcgacttgt gtagcataaa aaatcttcag gcgttttcct ttatctgttg
    47521 gtgttggatt gattgctaca gcatccataa tgatatcatt caacacagcc gaagagatac
    47581 gaagattctg gttcatactt acttgttcga tcatttcagg cagacgttcc aaccgttgtt
    47641 tagtcactgc agaaacaaaa acgattggag cgtaatcaag ataccggaat tcatcacgaa
    47701 tctcttgttc gaaatcacgc attgtattgg tttctttctt tacaagatcc catttattca
    47761 cgacgataat aattccacga ccagcttcat gggcgtaacc cgctactcgc ttgtcttgtt
    47821 cccgaatccc ttcttcagca ttcagtacca tcaaaacgat atcagaacgg tcaatggcac
    47881 gcattgcacg catcacgctg tatttttcag tattttcgta tacttttccg cgtttacgca
    47941 taccagctgt atctatcatt aagaatttct gaccactttc agaaacgaag tgggtgtcga
    48001 ttgcgtctcg ggtcgttcct tcaatatccg aaacaattac acgttcttct cctaaaatcg
    48061 cattgatcaa agaggattta ccaacatttg ggcgaccaat caaactaaac ttaatcgtgt
    48121 cgtcgtcctc ttcttcagtc tcttcagaga aatacttcac tgcttcatct aaaatatcac
    48181 caatccctag tccatggctt cctgaaacag gataaggatc gcctagccct aaggaataaa
    48241 attcatagat atcgtttctc atttctggat tatctacttt attgactgct aaaatcactg
    48301 gtttattact tctgtataaa attttcgcaa ccatctcatc tgcgtctgtc acgccttcgc
    48361 gtccgctgac tacacaaatg attacatctg cttcttcaat cgcgatttct gcttgatgct
    48421 tgatctgatc cataaaaggt tcatcgccaa gatcaattcc gcctgtgtcg atcacgctga
    48481 attctcgacc taaccattca ccttttgcat aaatacgatc acgagttact ccaggagtat
    48541 cttcgacaat cgagatacgc tcaccagcga tgcggttaaa aatcgtagac ttaccgacgt
    48601 tcgggcggcc aacgattgca attgttggat ttgccatatt atttcctcct cacaagtttc
    48661 caatctcact tagtttaacg aatgcattgc caactttcaa gcatagattg tcaattaaac
    48721 gaagtatttt tattccttat tgaaatacta atcgcttttc aaaagaaaaa ctgagacaaa
    48781 agcagctgct ttcatctcag tcacagatac aaaatcaatt atttttcaga atcatcggtt
    48841 gctttttctt ctacttcttc tttcaacgca tcacctaaaa tgtctcccat agtaaatcca
    48901 gtattttctt ctggtaattc ataagactca acattttttg gttcttcttc tgaagctggt
    48961 ttttcttcta aagctttgat actcaatgca atacgatgtt cttctggatg gatctctaat
    49021 actttcactt tcacttcgtc accttcatga agcacttcat gtggtgtcgc aatatgttta
    49081 tgtgaaattt gagagatatg gactagccct tctactccag ggaacacttc aacaaaggca
    49141 ccgaagcttg tcaaacgttt aactgttccg tctaatactg tgccaactgg agcttttgta
    49201 tcgatttctg accaaggtcc aggctgtgtg tctttgattg acaaggaaac acgttcttgt
    49261 tcaggattga tagacaacac tttgacattt acatcatcat tgacagccaa tacatcactt
    49321 ggttttgcta catgactatg tgaaatctct gagacatgga ctagaccgtc aattccgcct
    49381 aggtcgacaa atgcgccaaa atcagtaaga cgtgcaacac gtccactgag aacatctccc
    49441 tcatggatag ttgaaagaat ttcttttttc ttttcttctc tttcgttttc cactaccgct
    49501 ttatgcgata agatcaaacg attttctgat ggttcgattt cgatgatttt aaagctcaat
    49561 gttttgcctt tatagtctga aaagtctgca acaaaatgat cttccaccat tgatgcaggg
    49621 acaaaaccac gtacaccgac gtcaacgact aaaccgcctt tgacaacatt tgtaacaggt
    49681 gcttcgatga ccttgccctc ttggaaatct ctttcgatat cttcccaaac ttttttagca
    49741 tctaaacggc gttttgaaag tagatagctg ccgttttctt tatctttgcc gatcgtactg
    49801 ataacgacta aatctaatac atcaccgact tttactactt cgctgatatc ttctaccggc
    49861 aaagttgata attcttttgc cggaacgact ccttcaactc cagccccttc gattccgaca
    49921 atcacttgtt tgtcttcaat gactaaaact tcgcccttaa caacatcgcc aacttttact
    49981 tcttgtacgc ttttcattgc gtcttccatt gactggttgt tctcttgatt ttgatcaaat
    50041 tctgtcataa cacttgtcct cctaaccaac attctgccgt aagtacggta ctgtttttag
    50101 tttaattgat tattcaaaaa aaataaagcg atttcgttcg ttttccttga aaaaacaatt
    50161 gtttttcatt ttttttctta atttaaaaaa gaagataacc ttttttcaaa acaacagctt
    50221 ttattgctgc aacgacttct ggaatcgatt tgccagtcgt atcgatacga acagcatcct
    50281 ctgcttgctt cagcggagag acgtcccttg ttgaatcata atggtctctt tcctgtatct
    50341 ctttcgtcag tgtttcaaga tcagtcggaa ttcctttttc ttggttctct ttgaaacgtc
    50401 tttctgcacg ctcacttacg cttgctacta aaaagatttt cacttctgca ttcggtaata
    50461 cagctgtccc gatgtctctt ccgtccatga caacgccacc gcgttcccct atttttcgtt
    50521 ggacagcaac taattcttct ctgacttttc catgtgcaga gacttcagaa acagcttttg
    50581 tgacatctgg ttgacggatt tctaaagtca catcttttga tgctatgaat acatgctgtc
    50641 catcttcttg ttgcgaaaaa gagataggat actttttgat cagcgaaact agcccttctt
    50701 catctgaaaa agatacattg ttcgtaatag ctaaataagt tactgcgcga tacatggcac
    50761 cggtatctgt ataaatataa ttcaattctt ttgctaatat cttggcaacg gtacttttcc
    50821 cagatgaagc aggcccgtcg attgcaatac tgatttgtgt catgatataa ctcctttttc
    50881 tcagaaaaaa agccgcaaag cgcggctatt attatttaat tcttaattct tgaccagggt
    50941 aaagcataaa gttgtttgga tctaagccat tcaattcaaa caattgatca gtggtgatcc
    51001 ctgcacgctc agccacttgt tttggacctt cgcctgcttg aacagtcgtt gttgaaccgg
    51061 ctgcagctga tgaagaaggt gttgtttctg ctgcattgct gcttgaatcc cctgcttgat
    51121 aagttgcctc acttgaggaa gtcggtgttg tctctgctgc aatactgctt tctgacgtta
    51181 cacctgtatc ttgagacaca gtagattctt ccgcgattgt actagattct actgttgaag
    51241 tctcagttga ctcctctttt gttgtacttg tagaactttc tactactgat gtcgaagttt
    51301 cagaagaagt tgctgctgtt gcattgttgc ttgatccatt tctgatccag aaataagcac
    51361 ctgctggaat agcgatacat aagaccagta aaatcaaaaa tagcgaaaga aataatgtat
    51421 ttcctttctt ttgttgtctc tgagaacttc ttgatgaaac ttcatcatct gtatcgtaga
    51481 ttggttgttc ccatggttct tgtgtttcgt tattttctgt agattgatgt ctatcttttc
    51541 tgctcactgt tttacctcct aaactctatc gtgtgtatta taccatagga aaaagtgtct
    51601 tgtcctaaaa aatcaatgtt ccatgttttt ttaaataaat ttagtaaaat cgttccccaa
    51661 tgaagttgtt cagtcttaga aaaaagcttc ctctttggca gaagttctgc ttttgctcca
    51721 tcaatatcac aacaattctg cggcttttcg gagagttttt cgccaaaata agctaagatg
    51781 aattttctgc ggcagttttc ttcattaata tagcgaagca tctgctgcag gcggaattct
    51841 ttttcttttt cctgacgttt gagccgttcc acccaatttt ctggtttttc agactgctgg
    51901 atcagctcca tccatttttt ttgcagctca tcaaagggag cttgctctgt ggcatattcc
    51961 aaataaagct caaaactttg tcgctgttct ctagaaagtt gattaaagaa ataatggata
    52021 cgttcatccc cactttgata aaaaagaatt gctgcacttt cttcctgatc tctgcctgct
    52081 cgaccgattt cttgtacata gttttctaga ctgtctggca aatcataatg gaccacataa
    52141 cggatatcag atttatcgat ccccatacca aatgcattcg tagcaatgag aaactgcagc
    52201 tgatttttta caaactgctg ttgaagttgt cttctttgag ctgcatcaag accaccgtga
    52261 tagtatccaa ctgtaaaacg ttccctgaat aaatgataca atcgttcgac ttcttttttt
    52321 gttgcacaat aaatgattgc cgctccttgt gctctttcca tgaagtgttc aagttcttgt
    52381 tctttttgct gcgttttccg aacaaaaagc gagatatttt gccgattgac tgattgtctg
    52441 atctcttttg gtggtcgatc aaatagtaca tgacgtatat ctcttgctac taaatctgtt
    52501 gctgtcgccg ttagtgccag tgtgaccggg tttcctaaaa gctttcggat ttttcctaac
    52561 tgttgatatt ctggacggaa atcaactccc cattgagaca cgcaatgagc ctcatctact
    52621 acatataacg caatattttg ttttttcaga tgctctaaaa gagaaggatt agtcaacatt
    52681 tcgggactca aaaacaaaaa tttatattgc gatagatgac ggaggacgta tcttctttca
    52741 gattgcgaaa gtaaactatt gaaagcagct actcgtttct cccctcgttt ttgtaattgc
    52801 gtcacttgat cttccattag agaaagcagc ggtgacacga tgatgaccag accttccatt
    52861 aaataccccg tcaattgata gcaaaggctt tttcccgttc ctgtaggcaa aatagcgaga
    52921 gtatcttctc catcaagcaa cgcctgaata atttcttctt ggccgggacg aaatgaatca
    52981 aatccaaaat atttttttaa ttcatttatt aacattctgt atcgcttcct ttctcaatag
    53041 atagatttga tacaaacgaa attctccata atctaattgt ccatctacat tccattcgct
    53101 aaagcgccac tctcgcacgg aatccttatt tggttccaaa cgttcgattg tttctgcaga
    53161 gagaatccac tcgtatggaa aatcatcaaa aaacaaggcc cactcaataa gatgatctgt
    53221 tactgttcct ctttttaaat gacggattgc catgatctgg tcaattgtct gaccattcat
    53281 aaacatctgc ttggtgatca tcatgctttg attgaaattt tgctgtaata atgaatcgat
    53341 caaagcgtgc caaagcgaat ctggccgttt ttcgatttgt gctaacaaaa ggtggataca
    53401 tctgctttgt tgcagataaa ccgctgactc atcattgttt tttgccagct gataaggaag
    53461 cagccctgtt cgctcatttc cagataattg gttagctaaa aaatctgctg cttcttctgg
    53521 tatctccgaa aacagctgca ccaaactctc atacgcagta tcaatcaaaa tttcacgtgg
    53581 caattggctt tcactaagcc atttttttag ttggaaagta taaaaaggac ttgtctctgc
    53641 tggaagatag tcttgaatac ccgttgccag attagaaatt acttgaacag caaatttaat
    53701 gagccgccaa ctagtttccc ctgttctgcc atagcggtca aaacgcaaac cagtatgttc
    53761 tatttgatgt tcggacaaca tatcagcccc tgcaggcgtt agctttgctt cattttcatt
    53821 tatttcgatc caaccttgct gcgcaatttt ttgcaaaatt tgataaaact tatcctgctt
    53881 caattctgga aaactgttat ggataaacaa aagttcgtgg gtaaatccat aaataagtac
    53941 tgaagtcgtt cgttttccca caaggagttg gtaaagagaa ctaatccgca gcttgtgtcc
    54001 tgatgaaaat agcgataaaa taaaaacaat catattattc tccttaagtt aggatggtta
    54061 gaaatgtcac tactatgtaa acttgtacct gaacgctgta ttgcatgcgg tctttgtcaa
    54121 atcgaagcgc cagatatttt cgattatgat gaagaaggaa tcgttttatt tgcccaagaa
    54181 ccagaggctc atcaacagtt cgtgatgccg gaagaagaaa catatgtaaa aaaagcgtat
    54241 caaaaatgtc cagttcgagc gattttatta gaaaataaaa tataaatata aacgaaactt
    54301 taaaaacgta cactaagttc tctaaacttg aaaataagcc gggatctgtt tctcttgttc
    54361 cgtgaaatcc cgacttattt tgaaagattc atttgctatc tatagttaat tgactagttt
    54421 tttcacggac cctataggac ccatttatat tatttcaact ttttcagcca gttttccaat
    54481 gtattgacga tcacagtttg ctggtctgcg ttagaaattt ctgcctctcc atctcctttt
    54541 tgctcaccat agctgccaaa acctccatga tttccgccat tgattgatac atactctgtt
    54601 ctttccggta aatactgctt agcttcttga taagcttcct gattcaatac tttatctttt
    54661 gtcgccgtga tcgataaaac aggaacagct gattctgtca atgttccttt tttgtcggga
    54721 taacttgcca tgaagaatac cccttttaaa tgtttgtctg aatgattctt attgacataa
    54781 cgacttgcca ttacgccacc taatgaatgg ccaccgatca cataatcgga atttggaaaa
    54841 tcttctttga ccttatccgc tttgtttccg cccatcactg ctagatcaag tggcattttg
    54901 acaatagcga ctgaaaagcc gttttttgct aaatcttgcg cccaaatact ataactttct
    54961 ggctctacga gagcaccagg ataaaaaata agtaatggag actttccatg attctttttc
    55021 tctccttcaa aaaataagta atcttctttg ttttttcctt cttttaccaa cacttgcgct
    55081 tctgacgatg gttcgtacgt attttgctgt atatagtaat aaccggctcc accgagtaat
    55141 cctaaaatga tgactaagcc aattagtatt cgtctaaata ttttcacttt ctcccacctt
    55201 ttctatctga gtgacccctt tttcttaatc aagtatgatt ataccttaaa aaaataagag
    55261 agaaatgcaa actttccagc atcaaataaa aaaagacgga atatccattt gaatatccgc
    55321 cctttttgta tgtttatttt tatttagcac ataaacgata cacagcttct gcataaattt
    55381 ccattgcttt atacatacta cttaattccc atcgttcatt cgcctggtgc ataaagtcag
    55441 gagtatcagg gaacatcgca ccgaatgcca cacactggtt catcgtacga gcaaaagtag
    55501 cgccgcctga tacaaacggt tcagtcatgt ctccagtaat atcgcgatat gtccccaata
    55561 gatttttcac taactcactg tctagtggca cgtataatga atcaaggaag tcaaattctt
    55621 cataagtcaa cccatatttt tctgctgttt tagttaattt agctactaaa tcatctttct
    55681 tgaatgtaac agggattctc atatctacac cgatttttgt ttgttctggt gtgatttcca
    55741 aacttgcaaa gttcatcgtt agctcgccgg attgttcatc ttctgttttg cccacgacag
    55801 cttcaccagt tgcattttct tgaactaatt ttcctaagaa attgattggg ccaaaatcaa
    55861 atacttctga aagagcaatt gccagacgag aaatagcatt cactccttgt gctgcatctt
    55921 tcgcgtggat acttttacca agaacgacga tgccttcacc ttgttcttcg aaatcgaatt
    55981 cgtgtttttt caatgcttct tttacttcag aaagcttttc tccagagtaa ggagctgcat
    56041 caggaacaac attcaacgct cctccagctt ttacagaaaa ctcgttcgtt cctggtcctg
    56101 tcaagtaagc ttgcaataag cctttttctg cataaattaa aggaaattca gcatctggcg
    56161 caaatccttg cgtaattcct tcttcttttt cgttatattt ttctaaacaa cgccataaat
    56221 tttcttcatc tgtaccaaag ataaaacgaa tacgtgtctt gaattccacg ccagcatcaa
    56281 tcaaagcttt gacggcatac attgccgcaa tagaaggacc tttgtcatct tgggatccgc
    56341 gaccaactaa ccagccgtct tttatagttg gatcgaatgg ttttgtttcc caattatttt
    56401 cgtcccctgc tggaacaacg tccatatgac acaagatacc gaataattca tctccagagc
    56461 caatctctga ataaccgtaa tagccttctg gatctttaaa tgttctaaat cctagctttt
    56521 cactgatttc caatacttta tccaaagctt caatcacttt tttaccgaag ggatgtcctt
    56581 gtcctgaatc tgcttcatct agtacggaag gaattcggat caattcgctc aatgattcta
    56641 atgcttgttc atgatgttgt tctgtcacaa attttttcat gatattatcc tcctaatatg
    56701 ttaaaaaggg tcatgtcaaa ttcctccgac acaacctctt cttctcaaat aatgacgttt
    56761 taagcctgct ccatcagcaa gcagaatgtt cttaacaaat gatgaaagaa aaaacactca
    56821 ctgaatggag ctattgactt atccacgtgc ttatcgatag ccgtttatcg gtttcttcat
    56881 tcttatgata gaccttgtac ctgtatttgt ctaggtttag aacattgctg ctacacctag
    56941 gatcacacag atggctacaa ataatatcgc aattaattta ccggtaaatt tccaccacac
    57001 tgaaacatcg aaacgagcaa ttgctacagc acccattacg atagcagatg ttggtgtaat
    57061 taagtttact aaaccagatg cagattgata agcagtgatt actaaatcct taccgacgcc
    57121 ggcaaattca cccataggtc ccatgattcc cattgttgca gcagctaagc cggaagttga
    57181 aggaatcaag aatgacatag ggatatagaa aataaatgta ataatgatga atacacttga
    57241 agataatgaa cttaatcctt gttcgcccca atgtaagatg gtgtctgtga ttaatccatt
    57301 gttcataacc acttggatac cacgagcaac agctactacg attgctacac cgatcaaatc
    57361 agaagcacca gctaaaaatt cagagacaaa ttcagactct ttcatgccat aagtgattgc
    57421 tactacgatg gacattacta agaataacat agtaatttct gggaagtacc agtcacctaa
    57481 tggaagcatg cttttaccta gaaccgtacc tagtaccgga aggtttgtta accagtttgt
    57541 caatgattca aaaatcgtga aattcgcatt caaggttgtc caaggaatca aactgatgat
    57601 catgatacca aaagtaataa agaacaatac attaacatgt ttttgttttt tcgtgattac
    57661 ttcttgacga ccgattgctt caatatcgaa atgcttcata tcttctttac gttggttgta
    57721 tactaatgat tttgttggat ctttttcgat ctttgatgca taacgataca catatacaat
    57781 agaaatagct aacaaaataa cgaacatcaa gaatcgaaga ccaatacctt cacccatcga
    57841 aatcccaaca gcttgtgaag ctacgcctgt tgcaaaagga tttaccgttg aagctagaca
    57901 gcctacttga gaccctacta atacgattgc tacagctact actgtatcaa agcctacagc
    57961 catcatgact gggatcagta aagcatagaa tggaatcgtt tcttctgcca ttccgtaagt
    58021 agagccgcct aatgcaaata acagcatcaa tactggaata agcatttttt cttttccttt
    58081 gaatttcttc acaactgaag caatcccggc atctagtgcc cctgttttgt tgataatacc
    58141 taaaaatcca ccaacaacca aaatgaagaa agagattggg attgcagcag gtgtttcttt
    58201 agttagctcc cccgctggta cacctagcat cccattgatt ggcgccatga aaatatccca
    58261 cagcccttga ggattttgtt gtactcgttc atatgatcca gcgacgatgt tgcctgcatc
    58321 gtctacacta tagtgcccag ctggtataaa ccaagtaagt acagcgatta ctgcaataat
    58381 aataaataag accgtatacg ctgaaggcat ttgaaatttt ttcttttttg tttgttccat
    58441 cttcttcttc ctttcttttc cgttagagac aacaattttg gctctaatgt tattttatca
    58501 aaaagagtat cagaacaaaa ataataacac gcattttgta aaacttttgt tcaaaaagtt
    58561 cgtttttccc tattccctcc tcttttttca ggtttttaaa gctttcttct ttttcattgt
    58621 agcgtttcct tttttttata aacaaacatt ttcgtctttt aaaaggagaa gaatttcaca
    58681 tttg
//
LOCUS       DASJTC010000007        53415 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.007, whole genome shotgun sequence.
ACCESSION   DASJTC010000007 DASJTC010000000
VERSION     DASJTC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.007"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1158
                     /locus_tag="U4B19_000405"
     CDS             <1..1158
                     /locus_tag="U4B19_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEN2076174.1"
                     /translation="SVFGLGYVGLANSLLLAQKEQVKAYDIVEEKITMLQNGISPIED
                     KEVHEFLKRDDLNVEFTSNFTDAVNFGDYLIIATPTDYDEKKNYFNTSTVEDVIEKAL
                     AIRPDSTFIIKSTVPVGYANEIKAKFGTENIIFSPEFLREGKALYDNLYPSRIIVGEC
                     SARGREIATMFQRNAFNKDASVLLTHSTEAEAIKLFSNTYLALRVAYFNELDTYAEFR
                     GLDSKQIIEGVGLDPRIGSHYNNPSFGYGGYCLPKDTKQLRANYEKVPSNIISAIVDA
                     NTTRKDFIAGQILAKKPNVVGIYRLTMKAGSDNFRYSSIQGIMKRIKAKGIEVVVYEP
                     TLDEDEFYHSQVLKDINEFKNISDVIVSNRFEDILKDVEEKVYTRDIFGDN"
     gene            complement(2541..2807)
                     /locus_tag="U4B19_000406"
                     /pseudo
     CDS             complement(2541..2807)
                     /locus_tag="U4B19_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3645..4373
                     /locus_tag="U4B19_000407"
     CDS             3645..4373
                     /locus_tag="U4B19_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2076175.1"
                     /translation="MESSSKGKSLYYQLVDVLKERIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELETQGYIYRRHGKGTYVSDLSDKSSDLAGAYSFTEEMRELGRVPNTV
                     ILEFKQMEANKKVAKNLNINLGETVFKLKRLRLADDEPLMVERSYLPAKLFIGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDADPLRIADGAPVLYLSRTTYNVKN
                     EIIEYTDSVARADQFHYKIRHVRK"
     gene            4391..5584
                     /locus_tag="U4B19_000408"
     CDS             4391..5584
                     /locus_tag="U4B19_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2076176.1"
                     /translation="MFELSKDKLKELGAEITTREIKQQPQLWKETAEILAQNQEKLAL
                     FFDQIKKAANGKRVRVIFTGAGTSQYVGDTAVPYLNEYGDNDHFIFHSFGTTDIVSSP
                     KEFLFPDEPTLLVSFARSGNSPESVATVKIANEQIKTLFHLTITCAKEGQLAKNAATD
                     ERNYLLLMPEKSNDAGFAMTGSFTCMLLSALWVFDQTAVEKKESYLQQLIRTGEDLIS
                     RDQEIQKLLPKSFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVIGFVNNQPYTRNYDLDILEEIKDDQITTSVIAIAQPGNIDFTGTSLLLE
                     ASISLPDAYLALPMIMTAQIIALDTSIKVANKPDTPSPTGTVNRVVKGVIIHEYVNKG
                     ETKDE"
     gene            5577..6572
                     /gene="lacD"
                     /locus_tag="U4B19_000409"
     CDS             5577..6572
                     /gene="lacD"
                     /locus_tag="U4B19_000409"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEN2076177.1"
                     /translation="MNEEKIAAMKSLSTKEGIISALAIDQRGALKKMIKALGKEPTAQ
                     QITEFKELVSAELTPYASAILLDPEYGLPAAKARHQEAGLLLAYEKTGYDATTPGRLP
                     DLLADWSTLRLKEQTADAIKFLLYYDVDEDPEINHLKHVFIERLGSECAGEKRPFYLE
                     LVSYDAKTNDSTSLEYAKIKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVEGYSDG
                     EIAYSKKEAAAFFKEQSDATPLPFIFLSAGVSTALFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGREGAIEWLRTQGRQNIESLNKVINENATPWFDKVKEDSVTAK"
     gene            6569..7510
                     /locus_tag="U4B19_000410"
     CDS             6569..7510
                     /locus_tag="U4B19_000410"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEN2076178.1"
                     /translation="MITTVTMNPSIDIAYTLDELRIDQVNRVKTVNKTAGGKGLNVAR
                     VLHLLADDVLATGLVGGQHGAYIKEQLDQSGITHQFLPIKGETRDSIAILHGVNQTEL
                     LESGPVVTEEEQHKFTEHFARLMETTDIFTISGSLPQGFSKSYYAELLALTNKKGTRV
                     LLDTSGQTLLEVLEGKEKPFLIKPNLEELGELVHQTISSATSLDEIQSLLDHPLFEGI
                     NWIVLSLGAKGAIAKVGRHFYQVTIPKIAVVNPVGSGDSTIAGLAHALSKSYTIEETL
                     AFAMACGMANAQQEKTGWIDPVKVDYFRGEINVRKIR"
     gene            7556..9322
                     /locus_tag="U4B19_000411"
     CDS             7556..9322
                     /locus_tag="U4B19_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEN2076179.1"
                     /translation="MTRFEIKEEFLLDGQPFKILSGAIHYFRVHPTNWERSLHNLKAL
                     GFNTVETYVPWNLHEKEEGTYDFSKILDLSHFLELAQSLGLYAIVRPAPYICAEWEFG
                     GLPAWLLTKECRIRSADPRFMKYVKRYYQTLLPKIVPHLVTHGGNVLMVQVENEYGSY
                     GEEKAYLRQVKKYLEEGGIDVPLFTSDGPWQATLRAGILIDDDVFTTGNFGSKAKENF
                     ADMAQFFEAHGKKWPLMCMEFWDGWFNRWKEPIIKRDPEELADAVKEALQIGSINLYM
                     FHGGTNFGFMNGCSARGAIDLPQVTSYDYDAPLDEQGNPTEKYFALKRMMAENFPEMQ
                     QMEPWIKETIVKKGINLKEKVSFFATLDTISKPIHSTYPQTMEELGQNTGYLLYRTTI
                     KKDAVNEKLRIIDGRDRVHVFLDEEKQAIQYQTEIGETIPLTLEKEDHQIDLLFENMG
                     RVNYGHKLLADTQRKGIRTGVMSDLHFITEWTQYCLPLETTEHIDFSKKWEAGQPAFY
                     RFEAELSEIGDTYLDLSEFGKGIVWVNGTNIGRFWEVGPILSLFVPEGLFRKGQNEIV
                     IFETEGRFSEVIDFVKEPIEKS"
     gene            9339..9818
                     /locus_tag="U4B19_000412"
     CDS             9339..9818
                     /locus_tag="U4B19_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEN2076180.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNIGRIMVVDDEVATNAIEK
                     SGLKLATPAGVKLSILPIEKAAANILAGKYDSQRVLIVAKKPDRILALVEKGVPIKEL
                     NVGNMSQTDETRSITKSINVVDADVEVFKKLDQLGVHLIAQMVPNDKAEDFMSLLIK"
     gene            9831..10721
                     /locus_tag="U4B19_000413"
     CDS             9831..10721
                     /locus_tag="U4B19_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2076181.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDQHVETMNYKAIERNFLMGALPWALSRMVPVFLAL
                     AFGGGLVQNVVAVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHISYLILGFT
                     ITALLATVFSNVQLLGTSVASVVKDFPGTFNSLPMLAVALIGLALATISYKNDQKNDQ
                     HPVVSQQSQSDEGEIEDDEI"
     gene            10708..11517
                     /locus_tag="U4B19_000414"
     CDS             10708..11517
                     /locus_tag="U4B19_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2076182.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDDTPELKEMMKTHTQFFNTSNFFNTIITGIDLAIEEKEGVSSKETVSGMKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPVGILIWILAQVAVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKLLPAGIVGGVYWMLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            11533..11937
                     /locus_tag="U4B19_000415"
     CDS             11533..11937
                     /locus_tag="U4B19_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2076183.1"
                     /translation="MSRQLVLISHGKFCEELKNSTEMIMGPQENIYTIPLLPEDGADT
                     YKERFLSVTAPLKDYVVLCDLLGGTPANTVSKLIMEGETIELYAGMNLPMVIEFINSQ
                     MIGAEPDFIGSGRESIVSVNQVIAGMGDDEEE"
     gene            complement(12118..13056)
                     /gene="yidC"
                     /locus_tag="U4B19_000416"
     CDS             complement(12118..13056)
                     /gene="yidC"
                     /locus_tag="U4B19_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN2076184.1"
                     /translation="MNKWKNWLLGSGLVTIMLFLSGCVRMDSNGNPDTSGIIYRILVH
                     PMGQAITYLVENFNWSYGWAVILMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     ATQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESTFYGIDLGNPSLVLVAIAGIAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGIFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTPAEPAKQTKKQVQAPKNNAKNGRNAGKQQKRK"
     gene            complement(13390..14472)
                     /locus_tag="U4B19_000417"
     CDS             complement(13390..14472)
                     /locus_tag="U4B19_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2076185.1"
                     /translation="MTKNLIVRGAPQEYEIKENAWDSLPEHLYKRHIDNVLILHGKTS
                     WKAVKDVFPDLSITKIFEYYGGECTDPLADSFVSLCKKKDFNGIIAVGGGKVADLGKL
                     IAYKTNLPIIILPTLAATCAAYTPLSVVYNEEGVMLRYDMFPKANDLVLIDPKIILHS
                     PREMMIAGIGDTLAKWYESHAIISQLTIKPIEVQVAEFAALRCREILLEKSAEAMEAM
                     DQQKINQAFIDVVETNILLAGMVGGFGDDYGRTAGAHSIHDAMTVLPDSHQQLHGNKV
                     AYGILVQLMIENKLAEIEHLLPFYHHLNLPTSLADMGLHLTESDYQAVAERACLPDEM
                     IHFMKETITPALVVQKMKELEQVTQG"
     gene            14614..16065
                     /locus_tag="U4B19_000418"
     CDS             14614..16065
                     /locus_tag="U4B19_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN2076186.1"
                     /translation="MKKGINILLVALMFLLVFLPVKVQGAEKDEIYERIKQSGELVVG
                     LSADYAPYEFHANVDGKDKIVGFDVSIAEKIAKDMGVKLKVEELGFDALLGALKTGKI
                     DLVISGMAVTEERLQEVDFSDPYFVVQQKVLVRKEDKNKFKTTADFSGLAVGAQKQTT
                     QEELVKNELTGAELTSLQKVPDLINNLLNEKVEGVVLEQPVAEAYAQQNKKVALTPVK
                     FAQGEKTTAIAMSKNTPELKNHVNASIKTINDDGLLKDYQKQANQLMFQDDQSFLQKY
                     GPYYLIGTKYTILLAFVGVLFGMVFGSLLALMKLGKNKFLKALAIIYIEYVRGTPLLV
                     QIFIVYFGTGVLGLDLSKLVAGCIAVSLNSAAYVAEIVRAGINAVNKGQMEAARSLGM
                     DQKEAMRFIIFPQAIKNILPALGNEFVTIIKESSVVSVIGVSELIFQTGVVQGASFKP
                     FLPYVLVSLIYFVLTFSISRILALVERRMGLNA"
     gene            16058..16780
                     /locus_tag="U4B19_000419"
     CDS             16058..16780
                     /locus_tag="U4B19_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2076187.1"
                     /translation="MLNVKKLSKSFGENEVLKSIDLSVKEGEVVVIIGPSGSGKSTIL
                     RCMNLLEEPTSGEIFFKGQNITAPDSDINQIRQKMGMVFQSFNLFPHMNVLENLTITP
                     VKIKKEDPKKAKEHALSLLNQVGLKEKVDSYPTSLSGGQQQRVAIARALAMNPDVMLF
                     DEPTSALDPEMVGEVLAVMQDLAKKGMTMVVVTHEMGFAKEVADRVIFMADGIIQEEG
                     TPKEIFENPQNSRTQDFLNKVL"
     gene            16800..17951
                     /locus_tag="U4B19_000420"
     CDS             16800..17951
                     /locus_tag="U4B19_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2076188.1"
                     /translation="MDKSKIARRFQQPAENLLMNIAILAKEKEDIIDLSIGDPDLITD
                     PTIIEAAYTDVKNGATNYTAPDGSKEFLSTVVDFYQKKYGLSVECNQVRATVGAMHGM
                     YLVLQALLDPEDEVIIHEPYFSPYREQVILSGGKPVFVPTYEEKGFQLDVEDLKAAIT
                     DRTKVIVINSPNNPTGAVFSESTLKKIAEVAIENELYIISDEIYEDFAFKEKFVPMAR
                     FAPDNTITVSGVSKGFAMTGWRLGYMIAPAYINQIAGLINENITYSAPTASQRGAIYA
                     LKHYEQLVPPVVEVFKERLAYIEKRVAEIPYLSLSSVSGSMYAFINISKSGLDSVTFV
                     EEVLEKSGVLFVPGKAFGETIGDNYIRLAATQPLEILGEAFDRLAKLNF"
     gene            complement(18299..18574)
                     /locus_tag="U4B19_000421"
     CDS             complement(18299..18574)
                     /locus_tag="U4B19_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEN2076189.1"
                     /translation="MNKIKMNVQGRVQSVGFRYMTKIVADQLGITGTVKNEDDGSVTI
                     EAAGDDEAIKKFVEEVKRSPSPSGRVQYVDLQENPMMEDRKKFVVIG"
     gene            18682..19446
                     /locus_tag="U4B19_000422"
     CDS             18682..19446
                     /locus_tag="U4B19_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEN2076190.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKGEYLLEGFHLIEEAVAS
                     NAKVNELFITQRGLTEWASWLEENQALSVTLVSEEVMATVSDLPTPQGIMAVVEAEKR
                     EIPNFEGGWLLLDNVQDPGNVGTMIRTADAFGLSCVVLGKGTADVYSTKVLRSMQGSN
                     YHLPIIQEDLTKLLPILKEQGFITYGTELNEEAVSLNDIQTTENYAIVMGNEGQGVRP
                     EVLALTDKNVYIPIQGNAESLNVGVAAGIMLYHFSV"
     gene            19570..20073
                     /locus_tag="U4B19_000423"
     CDS             19570..20073
                     /locus_tag="U4B19_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2076191.1"
                     /translation="MTEKWREDEEYMSYINDLLETDAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYKLAKKWGGDARATARAGLLHDLFYYDWRTTKFDEGTHAYVHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMTASMKQKWQRY
                     FGKEQSI"
     gene            20338..20985
                     /locus_tag="U4B19_000424"
     CDS             20338..20985
                     /locus_tag="U4B19_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEN2076192.1"
                     /translation="MEKTIKPYQYYFIKREVEQLLSAYSSVNDPKTVQTVQALAAEKI
                     HEVLGHPNTAVNEFMEKVLDVTLKKEKAERLLEELKVIVLPFIQPTKPQIDKLFRKVK
                     KLKQPDWNQLDLREHTYIGWNDPGSQKKFMIYYEEEKLKGISGTLSPTINKGICSICQ
                     RTSNVSLFLSTTKTGSDGTYTKKGNYICHDSDQCNQQLVQLAPFYEFTERVKRER"
     gene            21146..22414
                     /locus_tag="U4B19_000425"
     CDS             21146..22414
                     /locus_tag="U4B19_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEN2076193.1"
                     /translation="MENRLPDPLKVIIYMIKWLLITAILGIFMGSLSAFFLTSLTDVT
                     AIRLEHPWLLFLLPISGGLFAYLYTTYGGNSSRGNNLVIDQGNGGNEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALAENFARWLKLNKIEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEALFPSFFAALFANFVTESFNVKHTHYQMGAIPAWDPS
                     LFFKLLLAGIAFGIIGWLFSRSIVWLKAWYAKRIPHPVLRNFIGGAVVVLVVVILQTQ
                     RYLGLSLPLLQDAFNGSAHPYDFLGKLGFTVLSLGAGYQGGEVTPLFEIGATLGATLA
                     PILHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGGQAASYFFMICLISFMCSGN
                     SGIYSAQKVEVKKGILFLPILGRISRQKEK"
     gene            complement(22591..23793)
                     /locus_tag="U4B19_000426"
     CDS             complement(22591..23793)
                     /locus_tag="U4B19_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN2076194.1"
                     /translation="MNLTRDLIFLVIYILGMSGILYWRKRQSDPALFFYCGTWTFMMM
                     VTYFVVLEVFYHTFYFLIPLLFFSIFAFFYFKEKRRLINGVLFNTFLIVFGGYLGWLL
                     IETQNIFIGGLLALIGIPILILLAIGIYILIIFLFWNGVVVMKKESRSLANLLTLLLG
                     VALTIFLILDYTLVHWLPQWANALFAVIPFIFAYLLIVFFNFMTVSILYQFNRPRYNQ
                     DYIVVLGAGLIDGERVSPLLAKRINKAIAFYWAQSKATLNPPVLLMSGGKGTDEKVAE
                     AIAMKQYALEKGIPERDILVETNSTTTLENMLFSKEIMDQQMNGPYRAIFTSNNYHIF
                     RAGLYARQAKLNANGIGAKTALYYLPNAILREFIAILVLHKKRHLIICGLMIGFAVFS
                     ALILKFAQ"
     gene            24320..25045
                     /locus_tag="U4B19_000427"
     CDS             24320..25045
                     /locus_tag="U4B19_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEN2076195.1"
                     /translation="MNKKRFKRIERKFALTYDQAKYLKTLFPKQVVRDKYSDHFISSL
                     YFDTNDHHFLKEITTEKSYREKFRIRKYGDPQDISPIYLEIKRKFQGMTDKRRIKLAH
                     HELDDMLENIELAETTFSNLEEEYIFNEIQWLFRRLALEPKVYIYYLREAFVNRDSED
                     LRITFDQNIHYRLMNLTTWEHCTEDNLVAPEIDVLMEVKTTGEIPEWLQVGLDRMGKK
                     EIKFSKYKQVYKRYIQEDPLYVQ"
     gene            25035..25709
                     /locus_tag="U4B19_000428"
     CDS             25035..25709
                     /locus_tag="U4B19_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEN2076196.1"
                     /translation="MFNSVLKNEPSQLTLDDFFLCMVVSILLGILIAYTHMYQNNCTK
                     NFMFALIGLPTLVHTVILLINGNLGTGIAVLGAFNLVRFRSAQGNSRDIFSIFWATSI
                     GLATGTGYIVYALFVSSIVAAIQVIIFTLPIKNKHTVFNTEKTIKLKVSKSLFLSSII
                     EEKIAEYTENCNLKKVKINKETCSLHYIVKVKTSMLEKELVRTIMDHPEVISITVGMD
                     KSNKLL"
     gene            complement(25997..26281)
                     /locus_tag="U4B19_000429"
     CDS             complement(25997..26281)
                     /locus_tag="U4B19_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 family protein"
                     /protein_id="HEN2076197.1"
                     /translation="MRQKCFLLLSHSQKVKPIFWLSIWDGFFKVSFHFPEKTREKLVT
                     LSLSEGTKEKIKTLQANGNKRKHLSVIFDVDHLEQLADIYQLAEFKKQNG"
     gene            complement(26575..27882)
                     /locus_tag="U4B19_000430"
     CDS             complement(26575..27882)
                     /locus_tag="U4B19_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEN2076198.1"
                     /translation="MFKKNTRFIVLLASLILCITSFLPSATVQADDAFKINAKAAFAV
                     DAESGKILYDQDGEKTMGIASITKIIGLYIVLEQIKEGKLSWEDKVAISDYAEKLSVV
                     PDLSNVPLHKENDYTVKELVDSAFIQSANASMVALAEKVAGSESKFLELMKKQLKDWG
                     IKDATIVNASGLNNSYLGENRPENTGETDENQLSAKDVAIVARHLILDYPEVLKITST
                     TNQTFGENTQSPVEMVNWNWMLPGFVNYHEGVDGLKTGTTDFAGACFVGTINKDGRRI
                     ITVVLNADNHEENPSARFNETSRLMDYCYDNWSEKELGKANASIPSLKDIKVKDGKET
                     SVNVALKSPVKVWVENGMDTGKLTITPTIDKKKVTDDQVTAPINKGTTVGSATIQLAD
                     DKLGYLEANQEPSTDIITTSTVEKANIFVIGWRHVTDFFSNLF"
     gene            28371..29477
                     /locus_tag="U4B19_000431"
     CDS             28371..29477
                     /locus_tag="U4B19_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076199.1"
                     /translation="MAKKEKELLSQLTSEGTKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKITSLLDDPSFWRSATTSVAVIVSPEQVFIHRLGI
                     PVDDQYYVSDLPYLLAIIKNGHFNYSYYLLGLNRDSMKLYEVTNKQVKEVALPEDAPR
                     DVVTALGSELTGGGLNYASNGSEGTTFHGVNPKDEEVEIDWVNYYQAVDDYLKDTFEN
                     PEKWPIFLFALPENQTMFKKVAKNPYYRSDVAISASPAQLSLKEIEAGAKKLSQELAV
                     KEASDYNKLLDKKFIDQLVDIIPAAKEGKISHLFIATSNLADGFGEDPETEYDRRQVL
                     NELADNVITNGGQVFILEQKDAPDEKSLVAILRY"
     gene            30068..30268
                     /locus_tag="U4B19_000432"
     CDS             30068..30268
                     /locus_tag="U4B19_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2076200.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            30415..33204
                     /locus_tag="U4B19_000433"
     CDS             30415..33204
                     /locus_tag="U4B19_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2076201.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIEAGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSSHTGMDTSRFIYHVLEEMKENPEPLDLSLEIIDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIMEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPGQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADVAHRGTGFLARNQSFYKQDFVRFLQKKIKQSNFLIVNHAFLVQETQRKEP
                     LLPASPYLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICVAEGLMNLVSSKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAMDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLLDFQAAMLPSTVWYPRYDHILYIGGTLKIGP
                     NRQYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLEAALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAIDEI
                     YQDIHEFLKKNEEGS"
     gene            33251..33778
                     /locus_tag="U4B19_000434"
     CDS             33251..33778
                     /locus_tag="U4B19_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEN2076202.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            33799..34989
                     /locus_tag="U4B19_000435"
     CDS             33799..34989
                     /locus_tag="U4B19_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2076203.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            35029..36327
                     /gene="asnS"
                     /locus_tag="U4B19_000436"
     CDS             35029..36327
                     /gene="asnS"
                     /locus_tag="U4B19_000436"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEN2076204.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVPEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            37261..37935
                     /locus_tag="U4B19_000437"
     CDS             37261..37935
                     /locus_tag="U4B19_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2076205.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWREDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQKAGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            37932..38273
                     /locus_tag="U4B19_000438"
     CDS             37932..38273
                     /locus_tag="U4B19_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076206.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVGQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            38303..38662
                     /locus_tag="U4B19_000439"
     CDS             38303..38662
                     /locus_tag="U4B19_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEN2076207.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     AFLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            39186..41267
                     /gene="cadA"
                     /locus_tag="U4B19_000440"
     CDS             39186..41267
                     /gene="cadA"
                     /locus_tag="U4B19_000440"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEN2076208.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNELIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            41578..43044
                     /locus_tag="U4B19_000441"
     CDS             41578..43044
                     /locus_tag="U4B19_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2076209.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            43354..43827
                     /locus_tag="U4B19_000442"
     CDS             43354..43827
                     /locus_tag="U4B19_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HEN2076210.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFIVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            43901..44341
                     /locus_tag="U4B19_000443"
     CDS             43901..44341
                     /locus_tag="U4B19_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEN2076211.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQKGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            44354..44563
                     /gene="copZ"
                     /locus_tag="U4B19_000444"
     CDS             44354..44563
                     /gene="copZ"
                     /locus_tag="U4B19_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEN2076212.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            44563..46749
                     /locus_tag="U4B19_000445"
     CDS             44563..46749
                     /locus_tag="U4B19_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEN2076213.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            46769..48940
                     /gene="copB"
                     /locus_tag="U4B19_000446"
     CDS             46769..48940
                     /gene="copB"
                     /locus_tag="U4B19_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEN2076214.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(49052..51223)
                     /locus_tag="U4B19_000447"
                     /pseudo
     CDS             complement(49052..51223)
                     /locus_tag="U4B19_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(51213..52154)
                     /locus_tag="U4B19_000448"
     CDS             complement(51213..52154)
                     /locus_tag="U4B19_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEN2076215.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            complement(52299..53147)
                     /locus_tag="U4B19_000449"
     CDS             complement(52299..53147)
                     /locus_tag="U4B19_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEN2076216.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
BASE COUNT        17418 a         9218 c        11219 g        15560 t
ORIGIN      
        1 tcagttttcg gattaggcta tgttggtcta gcaaattcac tattattagc acaaaaagag
       61 caggtcaaag cttatgatat cgtggaagaa aaaatcacga tgttacaaaa tggcatctca
      121 ccaatagaag ataaagaagt tcatgaattt ttaaaacggg atgacttaaa tgtcgaattt
      181 acgtcaaatt ttactgatgc agtaaacttc ggtgattact tgattattgc aactccaaca
      241 gattatgacg aaaagaaaaa ctattttaat acatcaacag tagaagatgt aattgagaag
      301 gctttagcaa ttcgtccaga ttcaacattc attattaagt cgacagttcc agttggctat
      361 gcaaatgaaa ttaaagcgaa atttggtaca gaaaacatca ttttttcacc tgaatttttg
      421 cgtgaaggaa aagctttgta cgacaactta tatccatcaa gaattatagt tggagaatgt
      481 tctgctcgtg gtcgcgagat tgcaactatg tttcaaagaa acgcatttaa caaagatgca
      541 tcagttcttt tgactcactc aactgaagca gaagctatta aattattctc gaatacatat
      601 ttggcattgc gagtggcgta tttcaatgaa ttagatacgt atgcagaatt tcgtgggctt
      661 gatagtaagc aaattattga aggtgtcggt ttggatccac ggatcggttc gcattataat
      721 aatccttctt ttggctatgg tggatactgt ttacccaaag acacaaaaca attaagagcc
      781 aactatgaaa aagtaccaag caatatcatc agtgcaattg ttgatgcaaa tacaacaagg
      841 aaagatttta ttgctggaca gattttggcg aaaaaaccaa atgttgttgg aatctatcgt
      901 ttaacgatga aggccggttc agataatttt cgatattcgt cgatccaagg aattatgaaa
      961 cggattaaag ccaaaggaat tgaagtggtt gtttacgagc ctacattgga tgaagatgaa
     1021 ttttatcatt cacaagtatt gaaagatatt aatgaattta aaaatatttc tgatgtgatc
     1081 gtatctaatc ggtttgaaga tattttaaag gatgttgaag aaaaagttta taccagagat
     1141 atctttggtg ataactaaat aataatgaac gggtacatgg aggaaaatat tttagatggt
     1201 gactcgaaat aaatgtttgt tctcattgca tctgtatcta taccgataag tttaaaaatt
     1261 ccgattagga aaatgaagtt ggctatcctt gcgaggaatt tattgctgga ttattttatg
     1321 aactaaagca agacatcatc agaaaaaaat ccaaaatttg acaatgggat tgatcgttta
     1381 ccagctgtaa gcgaagtatc tgacacgaaa atggaaaatg caattactgt tattaccaat
     1441 agcttttttt catcgatcaa gtatttgaaa acattatttg tttgccaagc tgatcaatgt
     1501 tctttcagac ttggaagaac attgatcagc tcacaagtaa tgattgaagt ttaggttatg
     1561 ggataaagga taagtgaaat aaaagtcgtg tctgcatctt tgttagtacc agtaaatttt
     1621 tctactacgg cgctagtcac gacagtccag gcatctgaaa ttgaagatgc aaatagagtt
     1681 tctgctagtt taggtattga tgttactgta ttagcattaa tcttagagcc agtagcatcc
     1741 aataggtgta gctatagttc taatagtata aaacaatgca gaggatatga ttgctttatg
     1801 tctcggtgtg tcaggtcaaa atagagttcc atatggggcg atttctcaat gaataatctt
     1861 atagatataa tcttgtttgc tatttcgtta tcacttttac ttttcttcga ttatttcaaa
     1921 aataaaaatc caaaatattt taagttctta ctatttatca ttactgtact agctgtttta
     1981 tcgatgacag attttattta acttatctca aaaagtaggg aaccatatca tttttcagta
     2041 acatgcgtat tatttgtata aataatacaa aatgaaaaat tatgattatt ctattgtctt
     2101 tttagaaagc gttttctgtg agcttttaat ctaaaaggag ggatataaaa taaagaaaac
     2161 ctattttatg ctattttttt gttgtttctg tgtaggaatc ctgtggtata taggtggtaa
     2221 tgaaaagaag attcatggag ttgtgacaaa tatttctgcg gatgagttcg tatttgaaga
     2281 tgaaaaacag aacgaatata gtattcaaaa aagaagctct ttgtcaaatg atgttggtaa
     2341 agagaaatct tagaataatt tggacagaag aggctgggac agaagtgttt aactccgaga
     2401 aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttcgg cttatttccg
     2461 aaggagttgc ttctgctccc gccgtttact cgagagtctg tagcaaaact gatttttaga
     2521 tttatcccac attcttctgt ttatttggtt aaaaatgttt tgccatgacg aatcatgatc
     2581 cgtagtttga aattgtaaaa gctgcgaaag ccatacgtat tgcgttttaa tactttgatg
     2641 tggttgttta aacattccaa tggtctgttt gaatagctgc agttaagcgc attttgaatc
     2701 tgtggcagaa aatttttaaa cgttcgtaaa gtggttgtat aggtttcagg cagatcgcta
     2761 atgtctctag ttaaacagtc ctcaaatagt gacgaatctc ttttctttca ctcaatgatt
     2821 caagaaggtt cttttaatat attggaagat gtggatattc ttctggtgtg tactgaagag
     2881 tccctttgta aatcaagcaa atttgtcctt tgtatttttc tttagttaaa caattttcct
     2941 cgaaatggat attgatattt aggtctagaa tatttagggt atctttgata agcagagtat
     3001 gagacatctt gaatcctcct atgaatttgt tgggcaaatt aattctaaag gatttcaggg
     3061 tgtcttttct atattttttt atacaaaaaa gatgttgata aagttcacaa acatcgaaaa
     3121 gtatagagcc cgaattttac gaatattttt tgtaatataa aagtatagat actaaattct
     3181 tattcgttaa acttacgcca taaggaaact agaaaaagac agaattatca taaaaaattt
     3241 ttgttatttt taaaaatgta caacaaaatt tcgactatca gaccggcatg agagaaaagt
     3301 tatctaaaaa ataaaaaatc ctactttatt tagagaaagc catttttatt tacatagtag
     3361 tctattaggc ccatttgctt gtcgatagta gaaatagcca cagatgtata aagaatattt
     3421 tttctttact tctctagacc atttagctga atctttgcca agattcagct tttttttgtt
     3481 ttttatttgt caactggtat ttttgaggta tcttttgata ccagttctaa attaccttga
     3541 aaccttgtta taatagcttt tttacgccaa aaaccctttg acaaatgagg aatatgtggg
     3601 tattcttgtg ttgtggttat aaccagtaaa gaagaggtat ttttatggag agctcaagta
     3661 aaggaaaatc attatattat caactagttg acgtattgaa agaacgtatc gaaacacaga
     3721 tgacacctca tgacaaattg ttatctgaaa gagaactttg tcagcattat ggagtgagtc
     3781 ggacaacggt tcgccttgct ttgcaagaat tagagacgca agggtatatt tatcgccgac
     3841 acggaaaagg aacatatgtg tctgatctat cagataagtc gtctgatctg gcgggagcgt
     3901 atagttttac agaagagatg agggaattgg gacgggtgcc taatacagtc atcttggaat
     3961 tcaagcaaat ggaagcaaac aaaaaggttg ccaagaatct aaacatcaat ttaggcgaaa
     4021 cagttttcaa attgaaaaga cttcgtttag cggatgacga accattgatg gtggaacgtt
     4081 cttatttacc tgcaaaacta tttattggtc ttgatctgcc attacttgaa agtaagccat
     4141 tgtatgatgt gttttctcag gattatgaac agcaagtcca attagcagat gaagaatttt
     4201 atgcaagtat cgttcaaaag aaagatgctg atccgcttcg tatcgctgat ggtgccccgg
     4261 tattatatct aagccgaacg acctataatg tgaaaaatga aattattgaa tatacggata
     4321 gtgtagcgag agcagaccag tttcattata agatccgcca cgtccgaaaa tgattgagag
     4381 ggaaaacaac atgtttgaac tatcgaagga taaattaaaa gagttgggtg cggaaataac
     4441 tacgcgagaa atcaaacagc aaccgcaatt atggaaagaa acagctgaga tattagcaca
     4501 gaatcaagaa aaattagcat tgttttttga ccagataaaa aaagcagcaa atggcaaaag
     4561 agttcgagtg atttttactg gtgcaggcac atcacaatat gtcggggata cagcagtacc
     4621 ttatttaaat gaatacggtg ataacgatca ctttattttt catagctttg gtacgacaga
     4681 tatcgtgtct tcaccaaaag aatttctatt tccggatgaa ccgacattac tggtgtcatt
     4741 tgcacgtagt ggaaatagtc cagaaagtgt ggcaacagtc aagattgcca atgaacaaat
     4801 caagacattg tttcatttga cgattacttg tgctaaagaa ggtcagttag caaagaatgc
     4861 agcgacagat gaacgaaatt atcttttact gatgccagaa aaatcaaatg atgcaggatt
     4921 tgccatgaca ggaagtttta cgtgcatgtt gttgagtgcg ttatgggtat tcgaccaaac
     4981 tgccgttgag aaaaaagaaa gttatcttca acaattgatc agaactggag aagatctaat
     5041 ttcacgagat caagaaatcc aaaaattatt gcctaagtca tttgaacgta tcgcctatct
     5101 tggttcagga agtttggctg gtgcaacgag agaagcacaa ttgaaaatat tggaattgac
     5161 agccggtaaa gtggcaacga tctttgattc ttctatgggc ttccgccatg gaccaaaatc
     5221 gtttttaaat agcgaaacac ttgttattgg ttttgttaat aaccaaccgt atacaagaaa
     5281 ttatgactta gatattctag aagaaataaa agatgaccaa attaccacga gtgtcatcgc
     5341 gatcgcccaa ccaggaaaca tcgatttcac aggtacaagt cttttgttag aagcttcgat
     5401 cagcctacct gatgcgtatc ttgccttacc tatgatcatg acagcccaaa tcattgcgct
     5461 cgatacgtcg ataaaagtag cgaataaacc agatacacca tcgcctacag gtacagtcaa
     5521 tcgagtggtc aaaggtgtca ttatccatga atatgtgaac aaaggggaaa cgaaggatga
     5581 atgaagaaaa aattgcagct atgaagagtt tatcaacaaa agaaggaatc atcagtgcat
     5641 tggcaattga tcaacgcggt gctttgaaaa agatgatcaa agcgttagga aaagaaccaa
     5701 cggcgcaaca gatcactgaa ttcaaagaat tagtatcagc agaattaacg ccttatgctt
     5761 ctgctatttt actagatcca gagtatggat tgccagctgc taaagccaga catcaggaag
     5821 caggactact tttagcctat gaaaaaacgg gttatgatgc gacaactcca ggacgtctgc
     5881 ctgatttatt agcggattgg tctacgctta gattaaagga acaaacagcc gatgcgatca
     5941 agtttttgct ttattatgat gtagatgaag acccagagat caatcatttg aaacatgtat
     6001 ttatcgaacg tttaggcagt gaatgtgcag gtgaaaagcg tccgttttat ctagaattgg
     6061 tttcgtatga tgccaaaacc aatgacagca cttcattaga atatgcgaag atcaaaccgc
     6121 ataaagtgat cgagatgatg aaagaatttt ctgatcctaa atataaagtg gatgtattga
     6181 aagtagaagt gccagtcaat atgaactatg tagaaggtta ttctgatggt gaaattgcgt
     6241 atagtaaaaa agaagcagcg gcattcttca aggaacaaag tgatgcaaca ccgttaccat
     6301 ttatcttctt gagtgcaggg gtttcgactg cattgttcca acaaacattg atctttgctc
     6361 atgaatctgg ttcaacattc aacggtgtgt tatgcggacg tgcgacttgg aaagatggag
     6421 tcaaagcata tgttgaaaat ggtagagaag gtgcgattga gtggttgaga acacaagggc
     6481 gacaaaatat cgaatcgttg aataaagtga taaatgaaaa cgccacccct tggtttgaca
     6541 aagtaaaaga agattcggtg acagcaaaat gatcacgaca gttaccatga atccttcaat
     6601 tgacatcgct tacacgttgg atgaattacg gatcgaccaa gtcaatcggg tcaaaacggt
     6661 caacaaaacg gctggaggaa aaggcttgaa tgttgctcgt gtccttcatt tattagcaga
     6721 tgatgtttta gctactggat tagtcggcgg gcagcatgga gcatatatca aagaacaact
     6781 cgatcaatca gggataacgc atcaattttt acctatcaaa ggggaaacga gagattcgat
     6841 tgctatttta catggagtga atcaaacaga actattggaa agcggtcctg ttgtgacgga
     6901 agaagagcaa cataaattta ccgagcattt tgcacgacta atggaaacaa ctgatatctt
     6961 tacgatttcg ggtagtctgc ctcaaggatt ttctaaaagt tattatgcag agttacttgc
     7021 cctaacgaat aaaaaaggaa cacgagtatt gttagatact tctggtcaaa ccttgcttga
     7081 agtcttggaa ggcaaggaaa aaccattttt gatcaaacct aatttggaag aattaggaga
     7141 gttggtccac caaacaattt cttcggctac ttcgttagat gaaatccaat cattattgga
     7201 tcatccgctt tttgaaggaa tcaattggat cgttctttcg ctaggcgcaa aaggagcaat
     7261 cgctaaagta gggagacact tctatcaggt tacgattcct aaaatcgcgg tggtgaatcc
     7321 tgtggggtca ggagattcga ctattgcagg cttagcacat gctctatcaa agagctatac
     7381 gattgaagaa acacttgcgt ttgcgatggc ctgtgggatg gcgaatgcac aacaagaaaa
     7441 aacaggatgg atcgatcctg tgaaagtcga ttattttagg ggggaaatca acgtcagaaa
     7501 gattcgttga ttataaaatt agaaagcggt ttctaaaaac gaggaggaaa tttgcttgac
     7561 tagatttgaa ataaaagaag agtttttgtt agacggtcaa cctttcaaaa ttttatctgg
     7621 tgccatccat tatttccgag tgcatccgac gaattgggag cggtcattgc acaatttgaa
     7681 agcgcttgga tttaatactg tggaaaccta tgtgccttgg aatctccatg aaaaagaaga
     7741 aggaacctat gatttttcga aaatattgga tctttcccat tttctagagt tagcgcaatc
     7801 ccttggatta tacgcaatcg tacgtccagc cccttatatt tgtgcagaat gggaatttgg
     7861 tggcttgcct gcttggctat tgacaaagga gtgccgtatt cgttcagcgg atcctcgctt
     7921 catgaagtat gtcaaacggt attaccaaac gctactacct aaaatcgttc ctcatctagt
     7981 cactcatggt ggcaacgtct taatggtgca ggtagaaaat gagtacggtt cgtatggtga
     8041 agagaaagca tacctgcgcc aagtaaaaaa atacttggag gaaggtggaa tcgatgtacc
     8101 gctattcact tctgatggac cttggcaagc cacattaaga gcaggcattt tgattgacga
     8161 tgatgttttt acgacaggga attttggctc aaaagcaaaa gaaaattttg cagatatggc
     8221 ccagtttttt gaagcacatg gtaaaaaatg gccgttgatg tgtatggaat tttgggatgg
     8281 ctggtttaat cgttggaaag agccgatcat taaaagagat ccggaagaat tagcggatgc
     8341 agtcaaagaa gcattacaga tcggcagtat caatctctat atgttccatg gcggaaccaa
     8401 ttttgggttt atgaatggct gttcagccag aggcgcgatt gatttgccgc aagtcacttc
     8461 ctatgattac gatgcaccat tggatgagca gggaaatcca acggaaaaat attttgctct
     8521 gaaacgaatg atggcagaaa attttccaga aatgcagcaa atggaaccat ggataaaaga
     8581 aacaatcgtc aaaaaaggaa tcaacttgaa agaaaaagtc agctttttcg caacactgga
     8641 taccatcagc aagccgatcc actcgaccta tcctcaaaca atggaagaat tgggtcagaa
     8701 tactgggtac ctattgtatc gaacaacgat caaaaaagat gcggtcaatg aaaaattacg
     8761 gatcatcgac ggacgtgatc gggtccatgt ttttctcgat gaagaaaagc aagcgattca
     8821 ataccaaaca gagatcggtg agaccattcc attgacactt gagaaagaag accatcaaat
     8881 tgatctatta tttgaaaata tgggacgggt caactatgga cacaagctgc tagctgatac
     8941 gcaaagaaaa ggaatacgta caggagtgat gagcgatctt cattttatca cagaatggac
     9001 acagtattgt ttacctttag agactacaga acacatcgat ttttcaaaaa aatgggaagc
     9061 aggtcaacca gcattttacc gctttgaagc ggaactgtca gaaatcggtg atacttattt
     9121 ggaccttagt gagtttggga aagggatcgt ttgggtgaat ggcacgaata ttggtcgttt
     9181 ttgggaagta ggaccaattc tttcactgtt tgttcccgag ggtttattta gaaaaggaca
     9241 aaatgaaatc gttatctttg aaacagaagg tagattcagt gaagttatag attttgtcaa
     9301 agaaccaata gaaaaaagtt agaaaaggag aatggatgat ggcaattatt ggagtaagaa
     9361 ttgatggacg tttgatacat ggacaagtgg caaatctttg gacgacaaag ttgaatattg
     9421 gtcggatcat ggtcgtagac gatgaggtag caacaaatgc gatcgaaaaa agtgggttaa
     9481 agttggcaac gccagctgga gtcaaattaa gtattttacc tattgaaaaa gcagccgcaa
     9541 atattttagc aggtaaatat gattctcaac gagtgctgat cgtagcgaaa aaaccagatc
     9601 gtatcttagc gctagtagaa aaaggtgtac cgatcaaaga attaaatgtc ggcaatatgt
     9661 cacaaacaga tgaaacacgt tccatcacaa aatcaatcaa tgtcgtagat gcggatgtag
     9721 aggtcttcaa gaaattagat cagcttggcg ttcatttgat cgctcaaatg gtgccaaatg
     9781 acaaagcaga agactttatg agtcttttaa tcaaatagga ggaatgattc atggatattc
     9841 aatggtggca gatattatta ttaacattat atgcagggta tcaaatttta gatgaattac
     9901 agatttattc ttcaatgagc gccccagttt ttgcaggatt atatgctggg ttagtaatgg
     9961 gtgatttaaa agcaggttta atcattggtg gaagtatgca gttgactgtt ttaggggtag
    10021 ggacatttgg tggtgcatca aaaatcgatg ccaactctgg aacgatctta gcaacagctt
    10081 tctccatttc tctagggatg aacccagaac aagcgattgc ggccatcgcg gttcctgtag
    10141 caagtttgat gatccaatta gatattttgg cacgttttgc gaatacgtat tttgcacatc
    10201 ggatcgatca acatgtggaa acaatgaatt acaaagcaat tgaacggaac ttcttgatgg
    10261 gggcattacc ttgggcactt tccagaatgg ttcctgtatt cttggcccta gcattcggtg
    10321 gtggcttagt ccaaaatgtt gtggctgttt taaacggcga cctaaaatgg ctaggagatg
    10381 gcttatctgt tgcaggggca gtcttaccag cagttgggtt tgcaatttta ctacgctacc
    10441 taccagtaaa gaaacacatt tcttatctga ttttaggatt tacgatcaca gcgttattag
    10501 caacagtgtt ctcgaatgtc caattgttag gaacaagtgt ggctagcgtg gtcaaagatt
    10561 tcccaggaac attcaattca ttaccgatgc ttgctgtggc attgattggt ctagcactgg
    10621 caacgatcag ttataagaat gatcaaaaga atgaccaaca tccagtagta agtcagcaat
    10681 cacaatctga tgagggggaa atcgaagatg acgaaatcta attataaatt gaccaaagaa
    10741 gattttaagc agatcaatcg tagaagtttg tttactttcc aattaggttg gaactatgaa
    10801 agaatgcaag gttcaggtta tctgtatact atcttgccac aacttagaaa aatctatggt
    10861 gatgatacac cagaattaaa agaaatgatg aaaacacata cgcaattttt taatacgtct
    10921 aatttcttta acacgatcat tactgggatc gatcttgcta tcgaagaaaa agaaggagtc
    10981 tcttcaaagg aaactgtttc tgggatgaaa gcaggattga tgggttcctt cgctgcaatt
    11041 ggtgattctg tatttgcggc attgatcccg accatatttg gagctttagc cgcaaatatg
    11101 gcaacagatg gtaatccagt aggtatcctt atctggatcc ttgctcaggt cgctgtcatg
    11161 gtctttcgtt ggaaacaact agaatttgcc tacaaagaag ggatttctct agttaccacg
    11221 atgcaatcac gcttgactgc attgacagat gcggctactt tactaggagt tttcatggtt
    11281 ggagcgttag tagcaaccat gatcaatgtt aaattctcat gggcaccaac gattggggat
    11341 gtaacgttga atatgcaaaa ctatatcgat atgatcttac ctaaactttt accagctgga
    11401 atcgttggtg gcgtttattg gatgttaggt aagaaaaata tgacacctac tcgagcaata
    11461 ttcatcgtat tgatcgtatg tgtagcatta tctgcattag gtgtgatttc gaaataaggg
    11521 agaattggaa agatgagtag acaactagtc ttgattagtc atggtaaatt ttgtgaagaa
    11581 ctaaaaaaca gtacagaaat gatcatggga ccacaagaaa atatctatac gatcccttta
    11641 ttgcctgaag atggcgcgga cacgtataaa gaaagattcc tctccgttac agcaccattg
    11701 aaagattacg tagtgctttg cgatctgttg ggtgggacac ctgcaaatac cgtttccaag
    11761 ctaatcatgg aaggtgaaac gatcgaacta tatgctggca tgaatttgcc gatggtcatc
    11821 gaattcatca attcgcaaat gattggagca gaaccagatt ttattggttc gggtcgtgag
    11881 agtatcgttt cagtgaacca agtgattgct ggcatgggag acgatgaaga agaataatca
    11941 agcacaactt cttgactggt gatacaactg gatataaata cggatgtata aagaggccgt
    12001 gtataaaaaa gccaacgtac cgcgaaatat agttttctat atttcacagt acgttggctt
    12061 ttatcgtcat aaaatcagaa atgatctggg tcgatcgaag aagctagtta caaataacta
    12121 tttacgcttt tgctgtttac cagcattccg accattcttc gcattgtttt ttggtgcttg
    12181 tacttgtttt tttgtttgct tcgcaggttc agcaggtgtg acatctttac gaggcgtcac
    12241 cacttgtttt ggcggatttt gttttaattc ttccgcaact tgcgctttta atcttggttt
    12301 catgatgaca ttcgtaatga atgtttgtag acaactaaag atcccaccga ctacccagta
    12361 aagagtgaca ccagctgggg cactgatcga catgaagacg atcatagcgg gtgatacgat
    12421 caacatcgta cgcatcgttt tcttttgttc ttcagggata ccgatagttg agatatagcc
    12481 ttgaagtaaa taagcaatac cggcaatcgc tactaaaact aagctagggt tacctaggtc
    12541 gatcccgtaa aaggttgatt cacggatgcc ttctgtatag cgagcagtga aatatagcgc
    12601 agaaaagatc ggcatttgga tcagaagcgg taaacaaccg ataccaccag tcatacttaa
    12661 gccattttca cgataaactt gttgcatttc agcttgtgca gccatttgtt cttctcgagt
    12721 tgtcgcagct ttcaattttt gttgcgtagc ttcaacttgt ggtttcaatg cttgcatctt
    12781 ttcagattgg atcattgttt ttttcgattg actaatacct aatggcaaaa tgatgatacg
    12841 aacgatgact gtcatcaaga taacagccca gccgtaggac caattaaagt tttccactaa
    12901 atatgtaatc gcttgtccca ttgggtgaac caatatacgg taaatgattc ctgaagtgtc
    12961 agggttacca tttgaatcca tgcggacaca accggacaag aaaagcatga tcgtgacaag
    13021 cccagagccc aataaccaat ttttccattt attcataaaa tacttcctct tttataattt
    13081 ttacacaagc ttaactatac taaaaaagcg ctcataattc aatcaacttc atagaaattt
    13141 catagaaata accgattgga ggatcaaaaa agacgcttaa tgttaaaaag aagaatccag
    13201 ctaaaaatac ttcagtataa agcatactta gcacctccgt tctctaaaat aagatgagca
    13261 acggatattt ccttgaaatc ataattttag cgcaaaaaaa agaagtggaa tgtcaatttt
    13321 ttccacttct ttttttttac cattgagccg atccataaaa aagctgactg ccatggtata
    13381 aggggctaac tatccctgtg tcacttgttc taactctttc attttctgga caaccagtgc
    13441 tggtgtgatc gtttctttca tgaagtggat catttcatca ggtaaacatg cacgttcagc
    13501 tacggcttga tagtcacttt ctgttaaatg taaacccata tcagctaaac tcgttggtaa
    13561 gttcaagtga tgataaaacg gcagtaggtg ttcgatttct gctaatttat tttcgatcat
    13621 cagctggaca aggataccgt atgcaacttt gtttccatga agttgctgat ggctatcagg
    13681 gagtacggtc atcgcatcgt gaatggaatg agctccagct gttcggccgt agtcatcgcc
    13741 aaacccacca accataccag ccaataaaat gtttgtttcc acgacatcga taaaagcttg
    13801 attgattttt tgctggtcca tcgcttccat tgcctctgca cttttctcta aaaggatttc
    13861 gcggcatcgt aacgctgcaa attccgctac ttggacttcg ataggcttga tagttaactg
    13921 agagatgatt gcgtgagatt cataccattt tgctaaggtg tcaccaatac cggcaatcat
    13981 catctcgcga ggggagtgca aaatgatctt tggatcgatc aacactaaat catttgcttt
    14041 agggaacata tcatagcgaa gcatcactcc ttcttcatta tagaccacac taagtggagt
    14101 ataagctgcg cacgtcgcag ctaaggtagg taagatgatg attggtaagt ttgtcttata
    14161 ggcaatcaat tttcctagat ctgctacttt cccgccgccg actgcaataa taccattgaa
    14221 atccttcttt ttgcataaag aaacaaaact gtcagcaagt ggatcggtac attctccacc
    14281 ataatattca aaaatttttg ttattgataa atctggaaaa acatctttta ctgctttcca
    14341 agatgttttt ccatggagaa tcaaaacgtt gtcaatatgc cttttgtaaa ggtgttcagg
    14401 gagtgaatcc catgcgtttt cttttatttc atactcttga ggggcaccgc gaacaattaa
    14461 attttttgtc atataaaaaa cttcttccat tattttttta aattaatctt tttttattat
    14521 aatagttatg ctagtatatt agcaagttat ttatgaatat ttattctttt atagcttttc
    14581 tgattacatt attattcaaa ggtggccaaa tggatgaaaa aaggaataaa catattatta
    14641 gtggcgctga tgtttctact ggttttttta ccagtaaaag tacaaggtgc agaaaaagat
    14701 gaaatttacg agcggataaa acaaagtgga gaattagtcg ttggcctatc tgcagactat
    14761 gcgccctatg aattccatgc gaatgtggat ggtaaggata aaatcgttgg ttttgatgta
    14821 tccattgccg aaaagatcgc aaaggatatg ggtgtgaaac taaaagtaga agaacttggt
    14881 tttgatgcat tattaggtgc gttgaaaact ggcaagatcg atttagttat atctggtatg
    14941 gcggtgactg aagaacgttt acaagaagtt gacttttctg acccatattt cgtggtacag
    15001 caaaaagtac tcgttcgtaa agaggacaaa aataaattta agacgacagc cgacttttct
    15061 ggcttagccg ttggggcgca aaagcaaacc acacaagagg agttagtgaa aaatgaactg
    15121 actggtgcag agttgacctc cctgcaaaaa gttcctgatc tgatcaataa tttgctgaat
    15181 gaaaaagttg aaggtgttgt gctggaacaa ccagttgcag aagcatatgc ccaacagaat
    15241 aagaaagtag ctttgactcc agtaaaattt gcgcaaggcg aaaaaaccac agctattgcg
    15301 atgtcaaaaa atacaccaga actgaaaaat catgtcaatg cgtcaatcaa aacgatcaat
    15361 gacgacggct tgctgaaaga ttatcaaaaa caagcgaatc aattgatgtt ccaagatgat
    15421 cagtcctttc ttcaaaaata cggtccgtat tatttgattg gaacaaaata cactatttta
    15481 ttagcttttg tgggtgtatt gtttggaatg gtctttggta gtttgcttgc actgatgaaa
    15541 ttgggtaaga ataaattttt aaaggcatta gccattattt atatcgaata cgtacgggga
    15601 acacctctct tagttcaaat ctttatcgtt tacttcggaa caggagtact gggattggat
    15661 ctatcgaaac tggtagcagg ttgtattgct gtctcgttga atagtgctgc ttatgtagcc
    15721 gaaattgtcc gtgccgggat caatgcggtc aacaaaggac agatggaagc tgcacgttct
    15781 ttaggaatgg accagaaaga agccatgcgt ttcatcatct tcccacaagc aatcaaaaat
    15841 attcttccag ctctaggaaa tgagtttgtc acgatcatca aagagtcctc agttgtttcg
    15901 gtcattgggg tatcggaatt gatctttcaa acgggcgtgg tacaaggtgc tagcttcaaa
    15961 ccattcctac catatgtgtt ggtctctttg atttatttcg tgttgacctt cagtatttcc
    16021 cgtatattag ctttagttga aaggagaatg ggtctgaatg cttaatgtaa aaaaactgtc
    16081 aaaaagtttt ggcgagaatg aagtcttaaa atcaattgat ctttctgtga aagaaggaga
    16141 agtagtggtg atcattggcc cttcaggaag tgggaaaagt acaattctac gttgtatgaa
    16201 tctgctggaa gaaccaactt ctggtgaaat cttttttaag ggacaaaaca ttacagcccc
    16261 tgacagcgat atcaatcaaa ttcgtcaaaa aatgggaatg gtatttcaaa gttttaattt
    16321 gtttccccat atgaatgtac tcgaaaattt gaccatcaca cctgtaaaaa tcaaaaaaga
    16381 agaccctaaa aaagcaaaag aacatgctct atcattattg aaccaagttg gtctaaagga
    16441 aaaagtggac agttatccta ccagtttatc tgggggacag caacaacggg tagcaattgc
    16501 tcgagcatta gcaatgaatc ctgatgtgat gcttttcgat gaacctacat ctgcacttga
    16561 tcctgaaatg gtcggtgaag tgttggcagt catgcaggat ttagctaaaa aagggatgac
    16621 gatggtcgtg gtcactcatg aaatgggttt tgctaaagaa gttgccgatc gtgtgatttt
    16681 tatggctgac ggcatcattc aagaagaggg aacaccgaaa gagatttttg aaaatccgca
    16741 aaatagtcga acacaggatt tcttgaacaa agtgttgtaa caaagatgga ggaagtaaga
    16801 tggacaaaag taagattgca cgccggtttc agcaaccagc agaaaatctg ttgatgaata
    16861 ttgctatttt ggcaaaggaa aaagaggata ttatcgattt atcgattggt gatcctgact
    16921 tgatcactga tccgacgatt attgaagcag cttatacaga tgtaaaaaat ggtgctacta
    16981 attatactgc gcctgatggc agcaaagaat tcttgtcaac ggtcgtagat ttttatcaaa
    17041 agaaatatgg attgagcgtt gaatgcaacc aagttcgagc tacagtcgga gcaatgcacg
    17101 ggatgtatct agttttacag gcattactag atccagagga tgaggtcatc atccatgaac
    17161 cttatttttc accatatcgg gaacaagtca ttttatctgg aggcaagcct gtttttgttc
    17221 ctacctatga agaaaagggc ttccagttgg atgtggaaga tttaaaagca gcgattacgg
    17281 atcgtacaaa ggtgatcgtc atcaattcgc cgaataatcc tacgggagct gttttttctg
    17341 aaagcacgtt gaaaaaaatc gctgaagtcg caatcgaaaa tgaactctat atcatttccg
    17401 atgagattta tgaagacttt gcctttaaag agaagttcgt accgatggcg cgatttgcgc
    17461 ccgataatac gatcactgtc agtggtgtat cgaaggggtt tgcgatgact ggctggcgct
    17521 tagggtacat gatcgcacca gcatacatca atcaaattgc tgggttgatc aatgaaaaca
    17581 tcacttattc cgcaccgact gcttctcaaa gaggtgcaat ctacgcctta aagcactatg
    17641 aacagcttgt accgccagtt gttgaagtat tcaaagaacg attagcttat attgagaaaa
    17701 gagtggcaga aatcccttac ttatctttat cttctgtttc tggaagcatg tatgctttta
    17761 ttaatatcag taagagtggg ctcgattcag taacgtttgt ggaagaggtt ttagaaaaaa
    17821 gcggcgtact ttttgtccct ggaaaagcgt ttggggaaac gatcggggac aactatattc
    17881 gactagcagc gactcagcca ttggaaatct taggtgaagc ctttgatcgt ttagcaaaac
    17941 taaattttta gataaaactg tatatttgaa aacagctatg ggtatccatt agaacaaaga
    18001 gggaaaatga tcctttcgag agttggcttg ttgactgggc agcgtcagga tttgacaatg
    18061 aaaaagtggt agtggaaaat caaagtcgat tttccactac cataaaatat tatagactcc
    18121 attttattga accaaaattt ttgaagtcct cccgctttta cgtatgccta tcactggtta
    18181 ctaaactcgc taataagaat gtttttttgt cgatctagga tggagagagg ggcaaacttt
    18241 ttcgatctac atacagacag tccgattttt ctgacatttc ctattcattt ctagaaggtc
    18301 agcctattac aacaaacttt tttctgtcct ccatcatcgg attttcctga aggtctacat
    18361 attggacacg tccgctgggg cttggtgatc gtttgacttc ttccacgaat tttttgattg
    18421 cctcatcgtc gcccgcagct tcgatcgtga cagagccgtc atcttcattt ttcactgttc
    18481 cggtgatccc taattgatcc gctacgattt ttgtcatata gcgaaaacca acgctttgta
    18541 cacgtccttg cacgttcatc ttgattttgt tcattttatt caccgttctt tcttttattt
    18601 ttaaattaat gataccgact tttttgctac tgaacaagtg caatgatcta atccatggta
    18661 taatcagttg aggtgaaaaa tgtgaaagag atccaatcag caaaaaatcc aagaatcaaa
    18721 gaattaaaaa aattacataa gaaaaaatat cgtgaagaaa aaggggaata tctgcttgaa
    18781 gggtttcacc tgatcgagga ggctgttgcc tcaaatgcga aggtcaacga actttttatt
    18841 acgcaacgcg gtctgactga gtgggctagt tggcttgaag aaaatcaggc gctttctgtt
    18901 acactagtat cagaagaagt gatggcaact gtttcagatc taccaactcc gcaagggatc
    18961 atggcagtgg tggaagctga aaaaagagag ataccgaatt ttgaaggtgg ttggttgttg
    19021 ctcgacaacg tacaagatcc cggcaatgta gggacaatga tccgcacagc cgatgctttt
    19081 ggtctatctt gtgttgtact aggtaaggga acggcggatg tctacagtac caaagtatta
    19141 agaagcatgc aggggagtaa ctatcattta ccgatcatcc aagaggatct aacaaaacta
    19201 ttgcctatcc ttaaagagca aggatttata acctatggaa cagaactgaa tgaagaagca
    19261 gtgtccctga atgatatcca gacaacagaa aattatgcga tcgtgatggg gaatgaaggt
    19321 caaggcgtac gtccagaagt gctggcgcta acggataaaa atgtgtatat accgattcaa
    19381 ggaaatgcgg agtctttaaa tgtaggagtt gcagcgggca tcatgcttta tcacttctcc
    19441 gtatagttaa ggaactatta agctttctgc attttgcgaa aacctcacta gaatattacg
    19501 aacactagtg gtatactaag ttcaatttca aagataaatg gaattcaaaa aggtggcaga
    19561 tcgataatta tgacagaaaa atggcgtgaa gacgaagaat atatgtctta tataaatgat
    19621 ctattagaaa ctgatgcagt aaagaaatta gcaaattaca cgcaacatgt ccactcgaca
    19681 cgattagagc attcaatcag tgtgtcttac tatagctaca aattagcgaa aaaatggggc
    19741 ggtgatgcaa gagcgactgc tcgagcaggc cttttacatg atttatttta ctacgattgg
    19801 cgtacgacga agttcgatga aggtacacat gcgtatgttc acccaaggat cgctgtgaaa
    19861 aatgctgaaa aaatcacgga gctttcagat ctagagcgtg atattatttt gaaacatatg
    19921 tggggagcaa cgattgcccc tcctaaatat aaagaaggtt atatcgttac ttttgtggat
    19981 aaatattgtg cagtcaaaga agcagctcaa ccaatgacag cttcaatgaa acaaaaatgg
    20041 caacgatatt tcggtaaaga acaatcgatt taaggtagtt aactaagtat ttgacccgag
    20101 tattttgaaa aaaatcctcg ggttttttgt ttagacattg actaattggt ctttttagtg
    20161 tttactttgt tgagatgaaa aagtacataa ccaacataaa aataaagggg gtgatgataa
    20221 gatgcgatgt gtatcttatt taaaagagtt tgagggcttt atctaaaggc actttgatag
    20281 aaagatcact tttttcttca aagtagccga ttttgaaaga agaaaggact caaagtcatg
    20341 gaaaaaacaa tcaaaccata tcaatattat tttatcaagc gagaagtcga gcagctgtta
    20401 agcgcctata gttcagtgaa tgatccaaag acagtccaaa cagtccaagc attagctgcg
    20461 gaaaagatcc acgaggtact tggtcatcct aatacagctg taaatgaatt tatggaaaag
    20521 gtactagacg tcactctaaa aaaagaaaaa gcagaacgac tattagagga attgaaagtg
    20581 atcgttcttc cattcataca accgacaaaa ccgcagatcg ataaattatt ccgaaaagtg
    20641 aaaaagttaa aacaaccaga ttggaatcaa ctggatttaa gggaacatac ttatattggt
    20701 tggaatgatc caggtagcca aaaaaaattt atgatctatt atgaagaaga aaaactaaaa
    20761 gggatcagtg gaacattatc tcctacaatc aacaaaggaa tttgttcgat ttgtcaaaga
    20821 acttcaaatg tttcactctt tctttctacc acaaaaactg gttcagatgg aacatacacg
    20881 aaaaaaggaa attatatttg tcatgatagt gaccaatgta atcagcagtt ggtacaatta
    20941 gctccttttt acgaattcac agaacgagtg aaacgtgagc ggtgatttca ttgtattttt
    21001 ctgaaaaata tgctatctta tttggtgaag tgaataggtg atggtgttcg cctttaaacg
    21061 ttgatcgtaa gataactaat gacgcctact aaagaaacgg ggctacccct ttttttagta
    21121 ggcgtttttt tgaggaggaa atgagatgga aaatcgatta cctgatccat tgaaggtgat
    21181 catctatatg atcaaatggt tattgatcac agcaatttta ggtattttta tgggaagttt
    21241 atccgcattc tttctaacta gtttgactga tgtgacagct attcggctgg agcatccttg
    21301 gttacttttt cttttgccga taagtggggg actttttgct tatctatata cgacgtatgg
    21361 gggcaattct tcccggggaa acaatttagt gatcgatcaa gggaatggag gaaatgaaaa
    21421 gattccatta cggctgatcc cactgacctt atttggcacc attaccacgc atctttttgg
    21481 tggttctgtt ggtcgggagg gtacagcggt ccaaatgggc ggggcacttg ctgagaattt
    21541 tgctcgttgg ctaaaattga acaaaatcga gcgtgagatt cttgtcatca gtgggatcag
    21601 cgcaggtttt agctcagtat ttgggacacc cttggctggg acattgtttg gattagaagt
    21661 attggcaatc ggaaaagtga gaaccgaagc actgtttcca agcttttttg cagcactttt
    21721 tgcgaacttt gtaacggaaa gtttcaatgt caagcatacc cattatcaaa tgggggcgat
    21781 tcctgcatgg gacccttcac ttttcttcaa attgttgctt gcgggcattg catttggtat
    21841 cattggctgg ctctttagcc gttcgatcgt ctggttaaaa gcttggtatg caaagcggat
    21901 cccccatcca gttttacgca actttattgg aggagcagtc gtggtactcg tagtagtgat
    21961 tttacagacg caaagatatc tcggattaag cttaccttta ttacaagatg catttaatgg
    22021 aagtgctcat ccttatgatt ttcttgggaa acttggcttt accgttctat cacttggcgc
    22081 gggataccaa ggaggcgaag tgaccccttt gtttgagatt ggggcaactt taggtgcgac
    22141 attagcaccc attcttcatg tatcagtacc ttttttagca ggattagggt ttattggggt
    22201 gttctctgga gcaacgaata caccgattgc ttgctttgtt atggggatcg aactttttgg
    22261 aggtcaagca gccagctact tctttatgat ctgtttgatt agctttatgt gttcaggaaa
    22321 tagtggaatc tattctgcgc aaaaagttga agtgaaaaaa ggaatcttgt tcctaccgat
    22381 ccttggaaga atctcacgac aaaaagagaa ataaaaagag gttgggacag aagtgtttaa
    22441 ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttta aatttttgtg aatttcgctt
    22501 atttccgaag gagttgcttc tgctcccacc gtttattcgg agttagagtc tgtggcaaaa
    22561 ctgattttta gttttgtccc acgctctttt ttattgagca aatttgagaa taagagcaga
    22621 aaatactgca aagccaatca ttaagccaca gatgataaga tggcgttttt tgtgtaagac
    22681 aaggatggca ataaattctc ttaagatagc attcggtaag tagtatagtg cagtttttgc
    22741 accgattccg tttgcattga gtttcgcttg tcgcgcatat agtccagctc taaagatatg
    22801 gtaattattt gaagtaaaaa tcgcgcgata aggcccattc atttgctgat ccattatttc
    22861 ttttgaaaac agcatatttt ctagcgtcgt tgtggaattc gtttctacta aaatatcacg
    22921 ctcgggaatc cctttttcta aagcatattg tttcattgca atggcttcag ctactttttc
    22981 atcggttcct tttcctcctg acatgagcaa gactggcgga ttcaaagttg ctttactttg
    23041 tgcccaataa aatgcgatcg ccttattgat ccttttagca agtaggggag atacgcgttc
    23101 accatcaatc aagccagcgc cgagaacaac gatataatct tggttatacc ttggacggtt
    23161 gaactgatac aaaattgaaa cagtcataaa attaaagaaa acgatgagta gataagcaaa
    23221 aataaacgga atgacagcaa atagagcatt tgcccattgt gggagccaat ggactaatgt
    23281 atagtccaat atcaaaaaga tagtcaaagc gactcctaac aagagagtca aaaggttggc
    23341 caaagaacgg gattcttttt tcatgactac aacaccattc caaaataaaa aaatgatcaa
    23401 aatgtagata ccgattgcca aaagaattaa tattggtatc ccgatcaaag ccaataaccc
    23461 accgataaag atattttgcg tctctattag tagccaccct agataaccac caaagacaat
    23521 caaaaacgta ttaaaaagta cgccattgat taacctacgt ttttccttaa aatagaaaaa
    23581 tgcaaaaata ctgaaaaata gcaatggaat caaaaaataa aatgtatgat aaaaaacttc
    23641 taaaacgacg aagtaagtga ccatcatcat aaaagtccac gtcccacaat aaaagaatag
    23701 agcgggatcg ctttggcgct tacgccaata gaggatgcct gacattccta aaatatagat
    23761 aactaaaaat atcaggtctc tagtaagatt cattgaatga actcctttcg atttgtaaat
    23821 tttttcttaa aaaagatagc atcatttagt agtaaatata tcgaatatag aaaagttagt
    23881 tactaaatag tcaatcagtt ttttcacgac gtttctatta taacgaatta tgttgaaaaa
    23941 agcttttatc gaaaacataa atcttcaaat taacagattt tttagacctc gatcatgatg
    24001 aagacttttg tagatacaaa taactgtaac attgaaaatg aatgcgaaaa cgatattttt
    24061 ttttattgga aaatatcggt caagcaataa accagttgca taataaaatg ttaacggcaa
    24121 caaaatctgt ggaaggaatg aaccggatga tttgatggaa aaaacaagat agacaagaag
    24181 tatacatagt aaaacgtgga acaagtaggc cagaatgaaa aatcagtttt tatccactcg
    24241 cttttctata cattcactgt cacaaatata atcgaaagta attctatttt gagataaagt
    24301 attttatagg agatagtata tgaacaaaaa aagatttaaa cgtattgaaa gaaaatttgc
    24361 attaacttat gaccaagcta agtatttgaa aacactattt ccaaaacagg tagttcggga
    24421 taaatatagt gatcatttta tttcttcctt gtattttgat acaaacgatc atcacttttt
    24481 aaaagaaatc actacagaaa agtcctatcg agaaaaattt agaatcagaa aatatggaga
    24541 tcctcaggac atatcgccta tctacttaga gataaaaaga aagtttcaag gaatgacaga
    24601 taaacgtaga ataaaattag cgcatcatga gctcgatgat atgttggaaa atatcgaatt
    24661 ggcagaaacc acattcagta atttagaaga agagtatatt tttaatgaaa tacagtggtt
    24721 gttcagaagg ttggcattgg agcccaaagt atatatctat tatttaagag aagcatttgt
    24781 aaatagagat tcagaagatt tgcgaatcac ttttgatcaa aatatccatt accgtctaat
    24841 gaacttaacg acctgggaac actgtacaga agacaatctg gtagcgccag aaatcgatgt
    24901 gctgatggaa gtaaaaacga caggagaaat acctgaatgg ttacaggtag gattggatag
    24961 gatgggtaaa aaagagataa aattttctaa atataaacaa gtctataaaa gatatatcca
    25021 agaggacccg ctctatgttc aatagtgtct taaaaaatga gccttctcaa ctcacactag
    25081 atgatttctt tttatgtatg gtggtgtcga ttttgctagg cattctgatt gcttacaccc
    25141 atatgtatca aaacaactgt acaaaaaact tcatgtttgc cttgattggg ttacctactt
    25201 tagtacatac cgtcattttg ttgatcaacg gtaacttagg cacggggatc gctgttctag
    25261 gcgcatttaa cttagtgcga tttcggtctg ctcaagggaa ttcaagagat attttcagta
    25321 ttttttgggc aacgtcgatt ggtttagcca cagggacggg atatattgtc tatgcactat
    25381 tcgtgtctag tatagttgct gctatccaag tcatcatctt cacgcttcca atcaaaaata
    25441 aacatacagt ttttaatact gaaaaaacga tcaagctaaa agtttctaaa tccttgtttc
    25501 tttcgagtat tattgaagaa aaaattgcag aatatacaga aaactgcaat ttaaaaaaag
    25561 taaaaatcaa taaagaaact tgttccttac actatatagt aaaagtaaaa acgagtatgc
    25621 ttgaaaaaga attggttcga acgatcatgg atcatccaga agtcatttcg atcactgtag
    25681 gtatggataa atcgaataaa ttattgtgat aaccaagaaa gtacttaaca atgaagtaag
    25741 accccagttc caaagtttag cggggacact tcaataaagt actttctttt ttgtcttcag
    25801 caactactgg taaaactgag agcaagcttt cgtgagagta cagcgataaa gatcaacctc
    25861 cctttttatc tcgagcaatt tccttgcaag gctgtttcgt tgatcttaaa cgagttagca
    25921 acagtcaaag atgcgatggc cataaaaaaa ttgcattaag ttggctggta tctgatagct
    25981 gggaatctaa ggaagactat ccattctgtt tcttaaattc cgctagttga tagatatcag
    26041 ccaattgttc cagatgatca acatcaaaga taaccgaaag atgtttcctt ttattcccat
    26101 ttgcttgaag agtttttatc ttctctttgg ttccttcact caatgagagt gtgactaatt
    26161 tctccctcgt tttttctggg aaatgaaagg atactttgaa aaatccgtcc caaatagaaa
    26221 gccagaagat cggttttact ttctgagagt gggacaaaag taagaaacac ttttgtctca
    26281 ttctttaaaa acgaataaac ggtgggagca gaagcaactc cttcggaaat aagccaaaat
    26341 tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttctcgga gttaaacact
    26401 tctgtcccaa cctcttgtat tcaccttttg atagccaagc ttttccatca ttataaaacc
    26461 gcaaatttca aaaaaaaacg aacaatacaa aaagcgtatt gctcgttttt ctcttcagtt
    26521 tctcacacct aaacgacttt tgtctcgacc tctttttggt gactattcat gatgttaaaa
    26581 taaattactg aaaaaatctg tgacatggcg ccaaccaatg acaaaaatat tagctttttc
    26641 aacagtagaa gtggtaatga tgtctgtcga aggctcttgg tttgcttcca aatagcctaa
    26701 tttgtcatca gctaattgga tcgttgctga gccaactgta gtgcctttgt tgataggagc
    26761 ggtcacttgg tcatccgtta ctttcttttt atcaattgtt ggagtaatcg ttaattttcc
    26821 agtatccatt ccattttcga cccagacttt tacagggctt tttagcgcaa cattgacgga
    26881 ggtttctttt ccatctttca ctttgatgtc ttttaggctt gggatgcttg catttgcttt
    26941 tccaagttct ttttctgacc agttgtcata acaataatcc atcaatcgtg atgtttcatt
    27001 gaatcgggca cttggatttt cttcatggtt atctgcattt aaaacgactg tgatgattcg
    27061 tcggccatct ttgttgatcg ttccgacaaa gcatgcaccc gcaaaatcag ttgtgccagt
    27121 ttttaatcca tcgacacctt cgtgatagtt tacaaaacca ggtagcatcc agttccaatt
    27181 gaccatttca accggtgact gtgtgttttc gccgaatgtt tgatttgtgg tactcgtgat
    27241 ttttaagact tcaggataat ctaagatgag gtgacgagca acaatagcga catctttcgc
    27301 agacagttga ttttcatctg tttcaccagt attctcaggt cgattctcac ctaggtaaga
    27361 attgtttaaa cctgatgcat tgacgatcgt tgcatctttg ataccccaat cttttagttg
    27421 tttcttcatt agctcaagaa atttactttc actgcctgca actttttcag ctaaagcaac
    27481 catggaagca ttggctgatt ggatgaaagc tgagtcaaca agttctttca ctgtataatc
    27541 attttcttta tgcaaaggaa cattcgatag atccggcacg acacttaatt tttctgcata
    27601 atccgaaatg gctaccttat cttcccaaga taatttaccc tctttgattt gttcaagtac
    27661 gatatataga ccaatgatct ttgtgatgga agcaattccc atcgtttttt cgccatcctg
    27721 atcataaagg atttttccac tttctgcatc tacggcaaag gcagctttgg catttatttt
    27781 aaatgcgtcg tctgcttgaa cagttgcgct aggcaagaaa gaagtgatac ataagataag
    27841 acttgctaat agtacaataa aacgggtatt ttttttgaac atctttgttc tcctcatttt
    27901 taaattgatt cttacaactt agaatattat cagagagaac aaaaggtttc aatggttctg
    27961 aaaaaaacct tataaagaac aagtcttatt aatgaagaat catagaaagt tatcttttgg
    28021 gttcattgac acgaactttg actggctgaa cttcgggttc ggttaggcgt gatgctgcaa
    28081 aaagggcgcc tgctgagagt gcgaccaaac taaaaatcat gaataattgc ataagatcac
    28141 ctcgttcttt tttgtttttt tatagtatag caagagagtt tgaagctact gttaaggaaa
    28201 tgagcatata attgatgaaa gtatgaaaaa aatatgaagt aggacaacac aactaaaaat
    28261 cgatcaggta tttctctggt agggattcgt tttattgtat tgataaaatg ggatgtggta
    28321 gactttattt acagatacaa agaaatgatt tttttaggag gggtacgttt atggcaaaaa
    28381 aagaaaaaga attattatca caattgactt cagaaggtac caaaggaccg tttgtcacga
    28441 ttatgttaaa cacccatgtt gcgcatcaag acgtagaaaa agatcaaatc aaattcaaga
    28501 attttgcaaa ggaagcgaaa aagcgctttg aaaagaaata ctctgagcat gattggtctc
    28561 cattccaaga aaaaattact agtttgttgg acgatccgtc tttctggcgt agtgccacaa
    28621 cgagtgtagc tgtcatagta tcaccagaac aagtgtttat ccaccgtttg ggtattcctg
    28681 tagatgacca gtattatgtg agtgatctac cttatttatt agcgatcatc aaaaatggac
    28741 atttcaacta ttcatactat ttattaggat tgaatcgtga ttcgatgaaa ttgtacgaag
    28801 taacgaataa acaagtcaaa gaagtggctt tgcctgaaga tgcaccaaga gatgtcgtca
    28861 ctgctttagg atctgaacta acaggaggcg ggttgaacta cgcatcaaat ggtagtgaag
    28921 gtacgacgtt ccatggagtc aatcctaaag atgaagaagt cgaaattgat tgggtcaatt
    28981 attatcaagc agtcgatgac tatttgaaag atacatttga aaatccagag aaatggccta
    29041 tcttcttatt tgcattacca gaaaatcaga cgatgttcaa aaaagtggcg aagaaccctt
    29101 attatcgttc cgatgttgcg atctcagctt cacctgcaca actttcatta aaagaaattg
    29161 aagcaggcgc aaaaaaacta tcgcaagaat tagctgtcaa agaagcaagt gactacaata
    29221 agttgcttga taaaaagttc attgatcaac tcgttgatat tataccggca gcaaaagaag
    29281 ggaaaatatc ccatttattc attgctacat ctaatttagc agatggtttt ggcgaagatc
    29341 cagaaacaga atatgaccgc cgtcaggtgc tgaatgaact agcagataat gtgatcacga
    29401 acggtggtca ggtctttatc cttgaacaaa aagatgcacc agacgaaaag agtttagttg
    29461 cgatcttacg ttattgagtg aaaactacca aagtagatga atggtcaggc gttgatagaa
    29521 gtgtatagct ccgagagaag gagtttcctc tgtttcagcc gttggattca tcttgagcga
    29581 gagacaaaag ctagcttttg tctctcgctc attatttttg tgctttagtc ccattggctt
    29641 tgcgagggga agatactttt ctcaaaaaaa cgaaccttgc agttgagagg gggatagatg
    29701 cgcatactaa tttttatcag ttaaaaaatg atgtggatca ttagagaatt ccaaagttcg
    29761 ttcatccttt ttcagataat aaattgaaat tttttgcaaa agtgaaaata gcaaatagaa
    29821 attttctgaa tcttataggg gtgatttcga gaaaacgttc gtttatttgc aaatatatct
    29881 tctaaataag agaataaata caaaaaaata aggaaaaact ctggcaattt tgtaaacggt
    29941 atgatatagt aagaatgtag tttttgttcg gtaatctgtg gtagcttgtt tcaaataaac
    30001 atcttccaga tatacaccat tcaagtaatt gcaaaatatg tgttgataca caaggaggat
    30061 ttttattatg gaaacaggta cagtaaaatg gtttaactca gaaaaaggat ttggattcat
    30121 cactgcagaa aacggaaacg atgtattcgt tcatttctca gctatccaag gcgacggatt
    30181 caaaacttta gaagaaggcc aagcagtaac tttcgacgtt gaagaaggtc aacgcggacc
    30241 tcaagcaaca aacgttacta aagcttaatt tacctatatt aaaaataaaa gtccagacat
    30301 ttatgtgtct gggctttttt gtcttttttt ccttaaaaag cgatgagagt atttttctgc
    30361 actttgtagt ataatttcaa aagaacatta gaggcaggag aagaggaaga tattttgaaa
    30421 ccaagaactt atgcagtcgt tgatatcgag acgactggga cgaatccaaa agaagatcgg
    30481 atcatccaat ttggctgcgt catgattgaa gctggtcgga tcacttcacg tttttcgatc
    30541 gatattcatc cagggcggaa gatttcaaaa caaatccagc atttaacagg gatcacgaat
    30601 caacgcgtcc aaaaagcgcc ttattttgaa gatgtcgccc aaacgattta caatcttttg
    30661 gcagacacca tatttgtcgc gcataatatt tattttgatt ataatttttt gaaccatgaa
    30721 ctcatgcgat gcggacttcc tagtttgaag ataccgggca tcgataccgt agaacttgca
    30781 caaattttct tgccgacgga accaagtttt cgcttagctg atttgtcaga aagccttggc
    30841 tttattcatg agaatcctca tcaggcggat agtgatgccg aagtgacagg tcagcttctt
    30901 ttactgatcg aagaacgaat gaggaaattg ccgatcatca cattagagca gattgcacga
    30961 ttaagtagcc atacaggaat ggatacgagt cgtttcattt accacgtgct tgaagaaatg
    31021 aaagaaaacc cggagcccct tgatctctca ttagaaatca tcgatgggct agctttgcag
    31081 aaaaaagaag tggagctatt cacatccgtc cattatggcg agcggacata tccaagaaaa
    31141 aaacaagcaa aagaaaaaat gtttggtaaa acactgatgt atcgaaaaga acagaacagg
    31201 ctgatgaatg ctgtctacga ccatttcaca aaagatgaat caaaagattt gatgatcgaa
    31261 gcagctacag gaatgggcaa aacgattggt tatctgttgc ctttgagtta tttggctaca
    31321 cctgaaaaac cagctgtcat cagcaccgtt tcgcttgttt tgcagcagca gatcatggaa
    31381 aaagacattc cattactgaa tcaattgcta gatcagccga tacaacctgt gattatcaaa
    31441 agctatcgcc actatattga tttacagcgt tttaaaggta cacttgtgga accgccggga
    31501 caaaaacaat atgctttgta ccaaatggct gtcttagttt ggctaacaca aacgaagacc
    31561 ggtgatttgg atgaattaca tttaaccaat ctgaaccatg cattctttgc agatgtcgcg
    31621 cacagaggca ctgggttttt agcaaggaat cagtcatttt acaaacaaga ctttgtgcgt
    31681 ttcttgcaaa agaaaatcaa acaaagcaat tttttgatag tcaaccatgc atttttagta
    31741 caagaaacac agcgaaaaga accattgctt ccagctagtc cttatttgtt gattgatgaa
    31801 gtgcatcatt tgccggaaat tgctgaaaaa gtaaatgaca aacgactaag tattaactac
    31861 ttccataaac aaattcagca actggaagag ccgaatcagt tatttgaccg catacaaagt
    31921 tatttgccga aggaacatga agccttgcgc tggttgtcga tttatcgaga agaactctct
    31981 gcactatgcg aagcgcagat ttgcgtggca gagggattaa tgaacttagt aagcagcaag
    32041 caacctaacg gacaatatcc agaagaagtc atgatgacaa aagaaatgtt ggatcagctg
    32101 ccactggaat ctggaacagc aatggatcat ctgcaacttt tttatcaaga gatccaacaa
    32161 ctacaatcac agatgcgctt gacttttgag attatccaag aaagttggac caaccagatg
    32221 cggatcgatt tagcaagttt atatagctta tttgatcaat tagacaagca atacttgctg
    32281 atggaccaat ggctagaaga ctggcgagat catttgatcc attggttcgt tcccgggaat
    32341 acagcacagc agatgatttt tcatctcctt gattttcaag cagctatgtt gccgtcaact
    32401 gtgtggtatc ctcgatatga ccatattctt tatattggag gaacactgaa gatcggacct
    32461 aatcgacagt atctctctaa acgacttggc attgaagcag cacctcttaa agtgattgct
    32521 tctccttatg attatgagaa acaggcaaga atctatgtgc cgaaagaagc acctgcagtc
    32581 catcaagtca gcaatcaagc atatatcgat tatttagaag cagcactcac aggattgatt
    32641 gaacaagaag atcggccgat ccttgtttta ttcacttctc atgatgtttt gcaaaaggta
    32701 tatcagaaaa tgcatttgtc tatgctagaa aaaggacgtg aaattcttgc tcaaggattt
    32761 ggcgggagtc gggaaaaact tttgaaacgc ttcattttgt cagaaaaaag cgtcttattt
    32821 ggtgctgaca gcttttggga aggcgtagat ttacctggag atgctttgca gattgttgta
    32881 gtcacacgtt tacccttcga caacccgtta cgtccgatgg tcaaagctag gaatgcttat
    32941 ctggaatcag aggggatcaa tcctttttat caggaatccg tgcctaaagc agctttgaag
    33001 ttacgccaag cattaggtcg tttgataagg acggagacag atcgtggcgt attacttatt
    33061 ttggatcgga gattagtcac tgcaaaatat ggtgcacgaa tcatccatgc attaccgaaa
    33121 aaagtaccga taaaagagct agctatagat gaaatctatc aggatatcca tgaattctta
    33181 aagaaaaatg aagaaggaag ttaaaggtgc cgtctttttt catcattaaa atctgctata
    33241 atgaaaagga atgaagataa aggagcgacg acccttgaag aaaaaacaga caaaggaaaa
    33301 aacgattaat cgcgtgttgc ttggcttgat cgtattttta ttagcggtca tcgtgatcag
    33361 ctcgattttt tacttgcgtt ccagtcgtcc ggcggtccag gcgaaaaaag aagcagtaga
    33421 aatcgcaaag aaatatgcaa atctcgagga agccgatcat ttttactggt ttactcgaaa
    33481 gaaaacgtac ttttcattga cgggtacgaa tgataaagga aaagaaatcg ccgttatcat
    33541 tcctaaatct ggtgaaaaag taaaagtatt ggatcaaaca aagggactga ctgaaaacga
    33601 agcaaaacag acaattgctg cggcccatcc tgaaatccaa atcgaaaaag ctgctttagg
    33661 gatgtatgat gatcagcctg tgtgggaagt agtaggaaaa gaaacaaatg ggaaactgaa
    33721 ttattacctg attggctttg aatctggaga tgaagtcaag acaatcaagg gaatttaaaa
    33781 ggtaaaggaa tgaatgggat ggagttatca aaaagaacaa aacgattaga accgtctgtc
    33841 actttggcag ctgcggcaaa ggccaaggca ttaaaagcaa aaggcagaga tgtgctgagt
    33901 ttgactgtgg gagaaccgga ttttgcgaca ccagaaaaca tccaagaagc agcaatcgaa
    33961 gcaatccgca atgggaaagc tagctattat acaccaacag caggaatccc agagcttcga
    34021 caggcaatcg ttgattatct gaagaaaaac tatggacttg tctatgaacc ttcacaaaca
    34081 gtcgtaacag acggagcgaa atttgctttg tatacccttt tccaaacgat ccttgacccg
    34141 caagatgaag tcatcattcc tgtgccttac tgggtcagtt acggtgagca agtaaaactg
    34201 gcagaaggac ttcctgtctt tgtgaaaggg gaagaatcca atagctggaa agttactgtt
    34261 gagcagctag aacaagcacg ttcagaaaaa acaaaagccg taatcatcaa ttctccatcg
    34321 aatccgactg gaatgattta tagcaagaat gaactgcagg caatcggtga atgggcagta
    34381 aaaaatgata ttttgatagt tgctgatgat atttatggcc gtttagttta taatggcaat
    34441 gagtttactc caattgcaac gatttcagaa gcaatcaaaa atcagaccat catcatcaat
    34501 ggcgtgtcta aaacatatgc gatgacaggc tggcgcattg gttatgcggt ggggaattct
    34561 gagatcatca atggaatgat tgctgttgct tcccagtcaa ccagtaatcc gacagcagtc
    34621 agtcaatatg cagctgttga agcattgaaa ggcgagcaag atacagtgga agaaatgcgg
    34681 aacgcctttg aagaacgcct gaatacgtta tatccgcttg tggcagaact gccgggtgtt
    34741 tctcttgaaa agccacaagg agctttttat ctttttccta atgtaaaaga aacgctacgc
    34801 atgtgtaaat atgaaaatgt cacacaatgg gtagaagatc ttttagaaga aacaggggta
    34861 gcgcttgtga ctggcgaagg ctttggggca ccagaaaatg tccgcatgag ttatgccact
    34921 gatcgcgcta cgttagaaga agcggtacgc agaatcaagc aatttattga gagtaaaagt
    34981 caaaattaat cctgaagaaa ttcaaggaat ataaaaggag agtcatacgt ggaacaaatt
    35041 caaatcatcg attcgaaaaa ccatgtggga gaaacagtaa caatcggcgc ttgggtcgca
    35101 aataagcgat caagcggaaa aatcgcattt ttgcaattaa gagacggaac agcctttttc
    35161 caaggtgtag ttgtaaaaag cgaagtgccg gaagaagttt tccaaacagc taaaaatcta
    35221 caccaagaaa catccgtgtt agtaactgga gaaatccgtg aagacagtcg ttcaaaattt
    35281 ggttatgaaa tcggaattac aaatgtggaa gtggtaagcg agagccacga ttatccgatc
    35341 acaccaaaag aacacggaac agacttcttg atggatcatc gtcatttatg gctgcgttct
    35401 tcaagacaac atgcgattat gcagatcaga aatgaaatca ttcgtgcaac gtatgaattc
    35461 tttaatcaac atcattttgt caaaatcgat ccgccgatct tgactggttc tgcaccagaa
    35521 ggcactacgg atctattcga aacaaattac tttgatcaaa aagcttattt atctcaaagt
    35581 ggccaattat acatggaagc tgctgctatg gctttcggaa aagtcttttc attcggtccg
    35641 actttccgtg ctgaaaaatc aaaaacacga cgtcatttga tcgaattctg gatgatcgaa
    35701 ccagaaatgg ctttcactca tcaagaagaa agtttagaag tacaggaaca atatgtagca
    35761 ttcttagtac aaaatgtttt agatcattgc gaatacgcat tagatgtact aggacgagac
    35821 aaagaagtct taaaaactta tacaaaattg ccattccctc gtattactta tgatgatgcg
    35881 atcgaattac tgaagaaaaa tggatttgat gacatcgaat ggggcgaaga ctttggttca
    35941 cctcatgaaa cgttcattgc gaattcattc gaacagcctg tctttatcct aaactatcca
    36001 aaagccatca agccattcta catgaaacca catccaacaa gagaagacgt ggtgatctgt
    36061 gcagatatga tcgcaccaga aggttacggt gaaattattg gtggaagcga acgggcaaca
    36121 gactatgatt tcttgctaga acaaatccgt aatcacggtt tggatgaaaa agaatattca
    36181 tggtatcttg atctacgtaa atacggttct gttccgcact ctggttttgg tctaggcctt
    36241 gaacgaacag tcacttggat tgctggtatc gaccatgtgc gtgaagctat tccattccca
    36301 cgtttgttga accgaattta tccataagag gttgtgaaat cagtgccttg acctgagtat
    36361 tagagaaaca aaacggaaaa atagcttctc atattttact gttttttgag ctaatatcga
    36421 gggtcagctg attgaacacc gtttatatca agaggttgtg cgtctgccgt tcagctccga
    36481 gtcacaagga gtaatttcaa aaaaacattt tttcatgttt gatgactatt gtgacttgtg
    36541 accaaggagt tggccggaga gcaacgttta tttaaaaaaa agaactgcga actctcgcag
    36601 ttcttttttt ttaaatacgg gataaaggaa gtttccagca gataagagaa gaaagcaaaa
    36661 gaaattgcta tttgattcac aaggttctat aacgaaatcg caatcaatca aacatataca
    36721 aagaaggtta ttcaaatgga aatctgattt gatgatgctt ctgctgttga aataaagcag
    36781 catctagcac ctgaaaacaa actaatagta acattcgaag atggagtagg tccgtatttt
    36841 cagcatgcaa tgatccatat gtaagttcaa ttcacccttg aaatcatcgg accagataaa
    36901 gttatggcca atctatccca agcaatctag aagagatatt agtaaaggaa catttactgg
    36961 aagtcttaga tgagcctata aatatatgtt ctgatggcgt catcaagtgg tctgattgac
    37021 ggaagcttag gtgtgatcga tctctctgga ccaaacaatg aaggaatcaa ggggaatcca
    37081 gcacgttaag aaaaaaatag cataaaaaac tagacacttg atgagcgttc attcaattgt
    37141 ttgaagggac taggctggtg tcatttttta tacatatatt ttctctttcg tcttgatagg
    37201 gcttatggga taatataagg aagaaaatca tcgttttatt caaaaaggag tttttgacaa
    37261 atgaaaaaac gtatattatt tatcggtgac agcattacag acgcaggaag agaccgagca
    37321 gaagcaactg atctaggtaa aggttatcca cttttgattg ccggaaaact aatctcccaa
    37381 tatccaacga tggaatttga atgcatgaac cgtgggatag gaggagacaa aattgcagat
    37441 ctgcaagtac gctggcgaga agattgttta gctttacaac ctgactatgt atcgattttg
    37501 atcggaatca atgatacctg gcataatact agcgatcagg ctatattcgg aactgagaaa
    37561 tctgctcagc aatttgaatc cgtttatcga aaattactaa atgaacttca aaaagcggga
    37621 atctgcaaca tcatcttgat ggagccgttt gtacttccta taaccgacga ccgtcatggc
    37681 tggcgtaaag acttagaccc taaaatccaa attgttcgta aacttgctaa ggaatatggc
    37741 gcattgctgg ttcctttaga tggcatattc aatgctttag ggattgctta tggtttttcg
    37801 aaatatacaa gagaagatgg ggtccaccca acgattggcg gacatgagat cattgcacaa
    37861 agctggatag atgcaattgc cgcagtcgat ttttttagtg gagataaaaa tgagcgaaag
    37921 aaaagtgaga aatgatggaa atacggtggg cagtaaaaga agatctacct caaatcaatc
    37981 agctgattac tgatcatcca gaatcattcg taaaagcaaa aacggcagct gacctaaatg
    38041 ataagaataa actattgctc gttgctgtaa caaaagaaaa acggattatc ggttttgcgc
    38101 aagcggacgt gcaacagtca gaagaacggg tgggtcagcg accatcagga aaactaatcc
    38161 aacacacatt agtaacaaaa gaaaatacgg aagaaatcca aaaaaaattt gcagaatatt
    38221 tgatgctgga agctaaaaaa agaagaataa aatcaatttt tgcccagttt taatcaaaaa
    38281 cagtaataga aggagaaata caatgagttg tttaggaaat gtcatctggt ttatattcgg
    38341 cggactaatc ggtggtctta gctggttagc agcggggata ctttggtgta tcacgatcat
    38401 aggtatccca atcggtatgc aatgttttaa gatcgccttc ctttcttttg cgccttttgg
    38461 caaagaagta atttatcacc agtctggcat gtcattgatc gttaatatca tctggctgat
    38521 cgtttcaggc ctaccaattg ctctcgcaca tgtcgtcagc ggcatccttc tatgtatcac
    38581 gatcataggg ataccttttg ccaagcaatc tttcaaactt gctcgattgg cgttgatgcc
    38641 attcggtgca gaaattgtat aaaagagaat aataaaggtt agataaagca tagagtcagg
    38701 tgggatacat gtatttcacc tgactctatg cgttttcctg tataagttct tccatgtgac
    38761 ttttacgtct tttatttata tggggttact gtttatggaa agttgaaata attttcatta
    38821 aagaactctt tctatatttt tgaaagcgta gtggagcttc gtatatctgg gaaggacatt
    38881 ttgcagggat tgaaaaaaga tattgtccta tcgaaaaatt aatgaattta gacaaattgc
    38941 cagcaacagg cttcaaactt agttgctatc ctgtcaagat caagaaggca agtgcgggtt
    39001 ggataagagc agtggcaatg atcgaagaga aaaagaaaga ataagccaaa acatctgtat
    39061 ggaagaaatt ttccgatgca gatgtttttc attttttctt tcggatacct tgacatatga
    39121 ataaccattc atttataatt agttatgaag agatattcat atgttgaggc gaggaggtca
    39181 ttaaaatgaa aagttacaga ttagaaggtt tagattgtgc gaactgtgca atgaaaatcg
    39241 aaaaaggagt tcagaaaatc aatggagtca aggaagcaac ggtaaacttc acttctggaa
    39301 aactaacgat tgacgcggag gaagatcatt tggctacgat tgaacaagaa acaaaaaaag
    39361 tagtgaagga attagaacca gatgtcaaag taacagaaat agataaggaa aaagtatctg
    39421 aacatggaaa tgaaaaagag agaaatacgc tgttccggat tttattttcc cttgcaggta
    39481 tcgctttatt gctattattt gattttaatg aactaattcg cttgataggg tatttattga
    39541 tttatctgct gataggatat gatgtagtga agaaggcagt gatgaatatc gtcaaaggaa
    39601 aaatctttga tgagaatttt cttatgtctg ttgctacgat tggcgctatg ttgatcggtg
    39661 aatatccaga agccgtagca gtgatgctat tttatcaaat cggcgagtat ttccaaggat
    39721 tggcagtcag tcattcaaga aaatcgattc gcgaattgat ggcgatccgt ccagaaacag
    39781 ctcacgtgca gacggcggaa ggattgatga ccgtgaaccc tgaagacgtg ctgatcggtc
    39841 agtttgtact tgtgaagcct ggtgaacgtg taccgttaga tggaacgatc atcgaaggag
    39901 aatcattagt cgatacgtca gcacttacag gagaatcagt ccctaaatct gtatatgttg
    39961 gtgaaacagt attaagcgga tttatcaata aaaataaacc atttttagtt caagtagaaa
    40021 aatcttatga aaattctact atcagcaaat tattagaatt agtagaaaat gccagcagta
    40081 aaaaagcacc agctgaaaat ttcattacta aattcgcgcg ctattacaca ccagtcgttg
    40141 taggattggc cgttttatta gctgttttac ctccacttgt tgtttctgga gcggcgtttt
    40201 ctgaatggat ctatcgagca ttgacatttt tagtgatctc atgtccatgt gcattagtga
    40261 tttccgtgcc acttagtttc tttggcggta tcggaggagc aagtaaaatc ggtgtattag
    40321 taaaaggttc aaattacttg gagttgcttg cgcaaaccga gacagtggta ttcgataaaa
    40381 ccggcacatt aacgaaaggc gacttttcga tccagacgat cgatacaaaa atcgatccaa
    40441 agatattcat gcaatatatt gcgagtgcag aacagttttc tacacatccg attgctcaat
    40501 ctgttttaga aggctacgca ggtccgcttt taccaacagc taatatccag gaatttgcag
    40561 gtgaagggat cttagctgaa gttgatggca aacaagtatt agtaggtaat cacaaattga
    40621 tggaaagatt tgaaatctct tttccttcaa gtcaagaaat cggtacattg ctttatctag
    40681 caatcgatca aagttacagt ggttatcttg ttattgcaga taccttgaaa gaagatgccg
    40741 tagacgcatt ggttcaatta aaacaggctg gtgtgaaaaa cacagtcatg ttgacgggcg
    40801 actcgaaaaa aattgctgat catatcggga agcaagtagg cgtggacaag atatatagcg
    40861 aattgcttcc agaagataaa gtgcagcggt tagaggaaat cttacaaagt aacaataaaa
    40921 agaccgcatt tgtcggagat ggaatcaacg atgcaccggt gctagcaaga gcagatgtag
    40981 gaatcgcaat gggtggattg ggaagcgatg ctgcaattga agcagcagat gtagtcatca
    41041 tgaatgatca accatcgaag attgcagaag caatccattt agcaaagaaa acattgaaaa
    41101 tcgtcaaaca aaacattgtc tttgctattg ggataaaaat tttggtactt tccttaggtg
    41161 cttttggttt tgcatcaatg ggagatgcag tatttgcaga tgttggggtg acagtattag
    41221 ctgttttgaa tgccatgcga agcttgcatg taaaaaataa acgttgatca atgaagagga
    41281 cgaatgaacg tcctcttttt tttatatcct tttcttaatg aaaaatgtaa agaaaataga
    41341 taggattata aaataactaa tgtttttaat tttgcagaaa attcaaaata gcttgaaatt
    41401 caataaggtc aatgaaataa aagaaaataa cttatttttt tgatggaaaa aatatgaaaa
    41461 aacgatgtgt attaaataaa ataatacaca cataaaataa cactgtcttg taagtttgtt
    41521 aaaaattgaa tatgattaca tctggataac aaatttttat cataaaggag agaacgaatg
    41581 aacaacaaac aattacgctg gtttactgtt gcgttaattg catttaacat ggtttggggc
    41641 atggggaatg tagttaataa ctatgctcag caaggagtat cagttgtcac ctcttggcta
    41701 ttgattttgg ccatttattt tattccatac gcattgatcg ttggacaatt aggatcagcc
    41761 ttcaaggata gtaaaggagg cgtcagttca tgggtcgaaa atacgacatc taataaacga
    41821 ctggcatact atgccgcttg gacttactgg gtcgtccata ttccttattt ggcacaaaag
    41881 cctcaagcaa ttctgattgc tggtggttgg gcagtccaag gaaatggaaa tatcgtaaat
    41941 acgatgtcag tacaaatggt agcagttatt tcacttatta tatttcttgc ctttttatat
    42001 ttatcaacaa aaggactttc tactttgaaa gtgattggcg gactggccgg aacagcgatg
    42061 ttcgtcatgt cgatcttatt tattctttta gctgtaacgg caccgttcat caatccaacg
    42121 atggaatttg ccacaccaca tatgaatcaa gtgaaaacat atattccaac atttgatttc
    42181 agttatttca caaccgtttc gatgctagta tttgcagttg gaggggcgga aaaaatatcc
    42241 ccttatgtca actctacaag aaatcctgca aaggaatttc ctaaagggat gatcttctta
    42301 gcagcaatgg tagggatttg tgctgtatta ggctcagtcg caatgggtat gctgtttgct
    42361 agcgataata tccctaaaga cttgatggca aatggggcat acagtgcttt tcaaatttta
    42421 ggtaattact atggtatcgg taactcattg atgatcattt acgcattgac aaacatgatt
    42481 ggacaaatct ccgcattagc cttttcaatt gatgcaccac taaaaatctt gttagcagat
    42541 gcggatccag aatttgtacc aaactggcta cgtaaacgta atcgtaaagg tacattggtg
    42601 aatggttatc ttctgacagg tatactagta ggaatcatta ttttgttgcc gatctttggt
    42661 atcaaagata tgaatgaatt agtaaaatgg ttgacgaact tgaactcagt tgtcatgcca
    42721 atgcgttacc tatgggtgtt cttggcatat atgatgttga ataaagctta taagaaattc
    42781 aattctgaat ataaatttgt taaaaatcca aaaatcggtt ttatctttgg tgcttggtgc
    42841 tttgccttca ctgcgtttgc atgtatccta ggaatggtgc caaaagtaga ttatactgct
    42901 gatcctaaag catggtggtt ccaattattc acgaatatca tcacaccaat tgtcttgatc
    42961 ttattaggta tgatcttgcc agcaattgct cgtcgagaca aaaacaaaga aattactgaa
    43021 ccagaaacag tggttcagcc ttaaaataga aatacttgtt acaagaggtt gtgacagaag
    43081 ctacttctgg aacaccgtga atcagaggtt gtgaaaaagc tgtcctgcat caagtaataa
    43141 gaacagccaa acaaaaatag ctcctcatat tttgccgttt tttgagctaa tgctgaaggt
    43201 cagctgattg aacaccattt attcgaaaca agggactttg acacgacttt tgtcacagtc
    43261 ccttgttttt ttgcggcgtt ataagtggta tgatcgaacc aaattccaaa gagaagtgag
    43321 ggagaagaat gaagatcatc gtgattggac aaagtgggtc gggaaaatcg tccctcgcaa
    43381 ggaagatcaa agaaatcacc ggctttccgc attcaggcaa aaaagtggtt tttgcaagaa
    43441 cagcaatcgt ttatagtatc taatccgtca tggatcgttg aaggaaatta tacttctacg
    43501 ttagaggaaa ggattaaaga agcagataag atcatttggc ttaaagtgcc gcgttatctc
    43561 gccatctacc gagtagtttg gcgttctcta aaaagaaaga taaacaaaaa atctcggcca
    43621 gatatgcctg aatcattttc ggagaaacta aacaggaaat acttagaatt tttatctttt
    43681 atctggcatt ttgaaggaaa caatgaacca aaaatacagc agctgatcca agtagctaat
    43741 gcaagaaata aactgatcat ccttaaaaca agacgagaga aacaagaatt tttaactaaa
    43801 ttgaaaatag agagcaaaaa taattgacag tttttgttta caagcgtaat ctgtattcat
    43861 aagctaatga ttacatttgt aaacgaaagg tgtgaaataa atggaaaaag tcaaaacgcc
    43921 agtaaagatc agtgattcag agtgggagat catgcgtgtt atttggacac ttggacagac
    43981 gacagcccaa gaaatcacgc agatcttagc tgattcgatg gattggaaac cagcaacgat
    44041 caaaactttg ctgggacgtt tggtaaaaaa agaagtgatc tggacagaac aaaaaggaaa
    44101 aaaattcatt tatcatccag ctgtctcgga gatggaaaca gttcgcagtg cgacggaaaa
    44161 tcttttttcc catatctgtg caaaaaggat tggagagacg attgcggatc tagtagaaga
    44221 agctactttg actcaagaag atatcaatga gattttgaaa caactggaac aaaagcaacc
    44281 agtagatacg atcgaatgta actgtatccc aggtcaatgt gaatgtaaac aacatcaata
    44341 ggaggaagaa aaaatgaaac aacaattttc aatcgaaggt atgagctgca atcattgtgc
    44401 agcaagagta gaagaaacag taagcgcact tgacggcgtg caaaaagtga aagtaaattt
    44461 gaaaaaagca aacggtacgg tcaagtttga tgaaacaaaa gttcaatccg aaaagatttg
    44521 tcaagcaatc aatggattgg gttataaagc agaggtgatt tgatggcaac agcagaaacc
    44581 aaaatggata cgttcgtcat tacaggtatg acttgtgcaa attgttctgc taggatcgaa
    44641 aaagagctga acggacaacc tggtgtggtc aatgccacag tcaacttggc cactgaaaaa
    44701 gcaagtgtca aatatgaagg gacgaccact gaaaaattaa tccaaagcgt agaaaacatc
    44761 ggttatggtg caattttgta cgatgaagcc cacaaacaaa aaattgcaga agaaaagcaa
    44821 gcgtatttga aaaaaatgct attcgatctt atattgagta cggttttgac tttgccgttg
    44881 atgttgtcaa tgattgctat gatgttagga agccatgcgg cgattgttca cttttttcat
    44941 tttccaatcg tccagttagt gctgtctgct cctgtccaat tttatgtcgg tgctcgtttt
    45001 tataaaggtg cgtaccatgc aatcaagacg aaagcaccaa atatggatgt attggtagca
    45061 attggaacat cggctgcctt tgcattgagc atttacaacg gatttttccg tggacacccc
    45121 caagatcttt attttgaaag cagcagtatg atcatcacgc tgattttgct cggcaaatat
    45181 ttggaacata cagcaaaaac gaaaacaggt aatgcaatca aacaattgat gtctcttcaa
    45241 acaaaaaccg cccaagtgat aagaaacggg aaagaagaaa cgttagcgat tgaagaagta
    45301 gtcgttggtg atcaactagt catccgtcct ggtgagcaga ttccagcaga tggtcgaatt
    45361 atttcaggaa gcagtgcgat agatgaaagt atgcttactg gtgaaaatct tcctgtagaa
    45421 aagaatccgg atgacacgct ttttggcggg acaatcaata cgaatggtct gctacacatg
    45481 gaagtcacac aagtcggcaa gcagacagta ttagcgcaaa tcattcaaat ggtggaagat
    45541 gctcaaggga gcaaagcccc aatccaaaaa atcgcagatc ggatctcagg gatttttgtt
    45601 ccgatcgtat tagtcatcgc gtttatcacg ctcatagcta caggtttgat cactggtgat
    45661 tggcaactcg cattgatcca tagtgtctct gtattagtca ttgcgtgtcc atgtgcatta
    45721 ggtttagcga ccccaacagc tattatggta ggaacgggtg ttggtgcacg aaatggaatt
    45781 ttaattaaag gcggagaagc attagaagca gctgctcact tagacagcat tgttttggat
    45841 aaaactggaa cgattacaga aggaaagccc aaagttacag atctagtcgg ttcaaaagaa
    45901 gtcctatcta ttttttatac tttagaacaa gcttctgaac atccactagg taaagcaatc
    45961 gtggaatacg gaaaactgca agaagcagca acatacgata tgattgattt tacagcccac
    46021 ccaggtgctg ggatcagcgg tacgattaat ggagtacgct attttgcggg tacacgtaaa
    46081 cgattaattg aattgaacct atcatttgat gaataccaag aacacgcgtt agaattagaa
    46141 cagcagggaa aaacagtcat gtttttagct gatgaaaagc aagtgatcgg gtggattgcc
    46201 gtggctgatc aaatcaaact agaagccaaa caagcaatca agcagctcca gaataaagga
    46261 ttagatgttt ttatgctgac aggagacaac aaactggcag ccgaaacgat tggtaaacaa
    46321 gttggtattg atccaaagca tatctttgct gaagtacttc cagaggataa agcagcctac
    46381 gtagaaaaac ttcagaaaga tggcaaaaaa gtcggtatgg caggcgatgg aatcaatgat
    46441 gcaccggcct tagcattagc tgatgtcggt atggcaatgg gaagtggaac ggacattgcg
    46501 atggaaacag ccgatgtgac gctgatgaat agcagtttgg catccattgc acaaacaatc
    46561 gagttgtcta ggttgacatt gagaaaaatc aaacaaaatc ttttctgggc ttttgtctat
    46621 aatacgattg gtattccttt tgccgcacta ggctttttga atccaatcat tgcaggagga
    46681 gcgatggcgt ttagttccgt cagtgtattg ttgaactccc tgagcttaaa tcgacacatg
    46741 aaaaagtaag agaagggatg gagtaaaaat gaatgggaac gatatggaga aaaaacatga
    46801 gcataaaaaa gaagtagtaa acaaagaaag tacagaatct catgaaaaac agatggagat
    46861 gaaacatgac catagtcaga tggatcattc catgcacatg ggacataacc atggagatat
    46921 agcaaacagc aaacaaatgg accattcgat gcacatggat cacgaacacg gcggcatgga
    46981 tcattccatg cacatgggga atttcaaaca aaaattttgg ctatcgctta tcctagctat
    47041 cccaatcatt cttttttcac caatgatggg aatggaattt ccatttcaag taacgtttcc
    47101 tggttctgat tggctcgtat tgatcttagc aactatacta tttatctatg gcggtcagcc
    47161 atttttaagt ggagctaaga tggaattgaa acaaaaaagt cctgctatga tgacactgat
    47221 cgcgatgggg attacagttg catatattta tagtgtctat tcgtttatcg caaatctgct
    47281 caatccacat acacacgtca tggatttctt ctgggaattg gctactttga tcgttattat
    47341 gctactaggt cactggattg aaatgaatgc tgtgtcgaat gccagcaatg ctttacaaaa
    47401 actagcagaa ttactgccag aatcagtgaa acgcttgacg aaagatggaa aagaagagac
    47461 agtttcattg aaagaagtaa acgaaggtga tcgtttgatc gtccggtctg gtgataaaat
    47521 gccaacagat ggtgtaatct tgaaaggtga aacaattgtt gacgaatcag ctgtgactgg
    47581 cgaatcaaga ggaatcaaaa aacaagccaa tgacaaagtg attggcggtt ctatcaatgg
    47641 ggatggaacg atcgaaatcg aagtaactgg aactggtgaa aatggttatc tagcgaaggt
    47701 tatggaaatg gtgcgaaaag cacaaggaga aaaatccaag ctagagtcat tatctgacaa
    47761 agtggcaaaa tggctgtttt atgtcgcatt aattgtgggt atcctggcat tcatcgcctg
    47821 gttgttcttg acagatcttc ctaatgcatt agaacgtatg gtaacagtct ttatcatcgc
    47881 ctgtcctcat gctctaggtt tagctatccc attagttgtt gctcgctcca catcaatagc
    47941 agctaaaaac ggattacttt tgaaaaaccg caatgcactg gagcaagcca atgatctaga
    48001 tgtgatcatg ctggacaaaa caggaacact aacagaagga aaatttactg taacgggtgt
    48061 cgaagtactt gatgatgcgt tcaataaaaa tgaaatcctt caatatctag gagccttgga
    48121 agcgaatgcc aatcatccat tggctgttgg cattatgaat tatttgaaag aacatgaaat
    48181 caagccttat caagctgaaa atctaaaaaa tctttcagga gtcgggctag aggcaacagt
    48241 caaaaaacaa caagtgaaaa tagttaatga aaaagaagtt gagcgtctcg gattaaacgt
    48301 agaacaagca ttattgaaac cttaccaaga acaaggaaat accatcagct tcctgatttt
    48361 agaaaatcat ctagctgcaa tcgttgcttt aggtgacgta gtaaaaacag aagcaaaaga
    48421 atttatccga acattgaaag aaagaaaaat cactcctgtc atgcttactg gtgacaataa
    48481 aaacgctgcc caagcagtag cagattattt agggatcgaa gaatattatg gcggtttatt
    48541 accagatgat aaagaagctg ttgtccagaa ataccttgac caagggaaaa aagtaatcat
    48601 ggtgggtgat gggatcaatg atgcgccaag tttagcccgt gcttcaatcg ggatggcaat
    48661 cggtgcaggg acagatatcg caatcgattc tgcggatgtc gtattgacaa acagcgatcc
    48721 aaaagatatt ttgcattttc ttgatctagc caagcaaacg cgaaagaaaa tgatccagaa
    48781 cctctggtgg ggggcaggtt acaatattct tgcaattccg ctagcagccg gaattcttgc
    48841 gccaattggt atcatattaa gtccagcagt tggtgctgtc ttgatgtcgt taagtacggt
    48901 agttgtagct ctaaatgctt tgacattgaa aatcaaataa taatgaaaaa aggttgtgac
    48961 aaaaaacgtg tcacaacctt tttttcgagt aatcgatact caatcagctg gttctcggca
    49021 ttccagaagt agattctcca gaaattttat cttatttttt gaagcggctc ccttttgtca
    49081 gaattttttt gtataccgtt tttcttatta tacgcgttat ttctaaatag gtttttttat
    49141 cgaattttac cggatctgct tgtcgtccat atatttctgc ttcatattga gtagttaatc
    49201 gaatgaaacg cagatctagc ttgaattctt gttctattcg caggctatat tcctgcaaag
    49261 attctgcggg tttgcgcggt accaatgttt ccaaagggtt aagaagtttt tgatatgcaa
    49321 tcgtattagt agaagcaaaa aaatgaagat agataagata acttgtccag cgcagagatt
    49381 ttctgaataa caatagaaag aacaacaaga cggataaaca cactccaagg ctgaataaga
    49441 cgattttata agctggtgtg agtgttgttt cttctgatgg aatgacctca gatctctcac
    49501 tggaaatagt ttctgccata ttttcgcttt catttggttg ttcgatagca ggctggacgg
    49561 tttcggttgt ttcagcaact ggttgtgaac ccattggatt ggcaaatgaa ggggttggtt
    49621 caaatggaag ccagccaaat ccaggaaagt atacctctgg ccaagaatga gcatgactat
    49681 tgagaatgct gtattcctca ttcccttgat tatctgtgcc tgttctgagc ccatttgtaa
    49741 aaccttttgt ccagcgagct gggataccaa gtgttcgaag aaggacgacc atagaagttg
    49801 aaaagttgtc acaatagccg acttttgaat caaagaggaa ataatccaca taatcgtgtt
    49861 ctttcggagt atgcccggta tccgttttgg aatagcggaa atttccgctt gtttttaaat
    49921 actgttagac agcttcgact ttacgaatta aagttgcttc atttttggtc aattcgaccg
    49981 ccaagtcctc aatacgttta ggcaaacttt ttggcaattg aagatttgtc tgctgatttt
    50041 ctaaatcatc agcttttaac tgactgcttt ttaattcttc tctcgtaaaa actaaccttt
    50101 ctgtttgtaa agttgcagaa cgtgtcgatt cattcgggac aggaatgaat cgctggcttt
    50161 caggcaaata gtaaagcgtt tgtccactag ctaatgaaaa atcagtcaag gtagtatttc
    50221 cataaggata aggtaagaaa gcttgttttt ttagggtcaa tgtaatgttt tctttgtctt
    50281 ctataacggt ttggtagctc gtgatgtcaa cagggaattc ttggattggt tggatgggtg
    50341 cgtcctgatc ttctatccat ccagaaccgg tgtaatgatt tttattttcg atcttccaat
    50401 agccatttcc tttttggttc gctgtaaaaa caacttcgcc attatcatat aaaggtccac
    50461 caagctgttc atcattctca ctgaagcctg ttcttgcact tccggttaat tgatactcat
    50521 ggagtgtgtc ataaagccct tcatcattaa gatattctcg gatcggttca ctcgtttcct
    50581 cgagagattg gtgtactttc ggaagatata aaggcaagat agcgctaaaa gttaagaaac
    50641 aaataagcag aactgtagtc aatgactgga atcgagtgtt tcccgacaac aaccggatgg
    50701 agaaaaattg ccttgctgcc agatacacga acaaaacagt tactaccgca atcatttcgt
    50761 acagtaaatc ttttttccga aaaacggtaa gcatcatgaa gtaagccatc aaagaaagaa
    50821 agggtgcgta ccaatagtcg taatccacgg tcaatgtaat aaataaatag atcagcaaca
    50881 ttatgagaaa aaaagctgtc ttttctggca aataataaag tttaccttgg gatagatgga
    50941 agaattcctc tgtaactgac gaaaaaaact gtaagatcca taatatggaa aatgtcttcg
    51001 aaggaaaata taaaaacaaa caacctatca ttactggcaa agaaagcagc aaacgtaccc
    51061 attttttagg aatacagcag acaataaggc ataggacacc aatagttagg ataagaaggt
    51121 agctgttttg caattgatta gctactacaa atatcggagc acctacactt agtatcacaa
    51181 aggagtaaac gccacgcagc catttttccc gtttaatcgt caatccattc acctcctggc
    51241 aatgaacata tcttgtcttt ttcttggacc atgagttgtt tttctacgaa ataaacaaga
    51301 taagccgttc gatcttttga caatgtttca atagcttctg agacttcagg agaattctca
    51361 ggaacaaaga ggacgatttg tttttttgca tattttttca agtgtgcgaa aaaagctttc
    51421 tcatcgccag cttccaaaga agcaaaatag gtatgaccgg ggtctttgcc atgaaaggcg
    51481 cggttgccta atatcagaag ttccggctgc ttttcttttc tcaaatgcca taaagaaaaa
    51541 tacaagtcta gcagaggttc aaattttgga ctgggcgaac cccaaaagca aaaaagagga
    51601 gaacattctt cttcttgata atcgtattca cggtataacc actcttgagt atgagcagac
    51661 aatttccaat cgattcggtt gaaagaatca cctggacgat aagcgcgatg ttcccgaaaa
    51721 tcaagacctc tttggtacgc gttcagctgc tgtttttttt ccagaactct ctgtaatttt
    51781 tctgcttgaa tcttacaaaa agcaggcaga acagtcacag gaaaagagat ctctcttttt
    51841 gcactttttc tgaaaagtcc tagactatca taggctgtaa aaatgacagt taatgtttca
    51901 tggcatcctc tttcttttgg ctgccaaagc aattggactt cttgcggttt atttgtaaga
    51961 atcaaaatct cttgtttttt tctgcaaaga ttgctgggaa gccgatttgt aaacgaggga
    52021 gaggtaaaga aaaagttctc ttttttgcta tggaaaaaat aacctgcata ctttttttgc
    52081 gataaacgag tggctgcaat gtacaagtaa ccgaaagttt ctttagaaag ctgcttaatg
    52141 aaatcaagct ccatgataca taaagagtaa aaaaggccaa caagaaccag cctaaagaat
    52201 tgttgaatac aagagcaaaa agcacaatga tgaaatatac cagaaataac acaaaattaa
    52261 tgaaataatc tttgattttt tccattgtct acctcaatct acctcacagg aataggaatt
    52321 tgtgcaacaa gagtatgaat gatttgtttc ttttcgtctt ccttcaatga acgatcgtaa
    52381 aaaatcaggc gatgggaaaa cacgtaagga actaaatcct gcaaatcttt gggcgtgacg
    52441 taatttctcc catggattaa agcataagct tttgctgcct gaataaagga aaggcttcct
    52501 cgtggactga tgccaagctg gatggcagca tgacttcttg ttgctgacac taactgcaag
    52561 gcataagatg ccacttcggg actgacaaag acattcgcca catttgtttt tatttcttct
    52621 atttcataac tgttcaatac ttcatttaag ttatccaaca ctttttcgaa tttatccaaa
    52681 agtagcaaaa gttcgtcatc aaaagaagga taaccgattt gtagtcggaa taaaaaacga
    52741 tcgagctgtg cttccggcaa aggatatgtt ccttcgtact cgattggatt ttgcgtagct
    52801 aagacaaaaa aatgattgtc tagagggtat gtattgttgt cgatcgttgc atgattctcg
    52861 gccattgctt ctaaaagtgc tgactgagta cgaggagtcg tacgattgat ctcatctgcc
    52921 aacaagatcg tggtaaagat cggtcccgga cgaaattcaa actcttttga ttgtgaattg
    52981 tatacggaaa aaccaagaat atcactaggc agcagatcag gagtacattg gactctgctg
    53041 aaggttccct gtacagcttt tgctagtgct ttgaccagta acgttttacc tacaccggga
    53101 atatcttcga ataagacatg accacctgct aatagggcca cgatcatcaa ttggatagtt
    53161 gaacgtttgc cgatgaccgc tttttctaat tcttatatca actgatcgat tttttgtttg
    53221 gattcttcta ttttttcttc cattttcaca tcaccttctc ataaaatcgc tatttatttc
    53281 attgtaatgg aagggaatac atcctacaag caaagtgatc ttattatgtt tttttaaagt
    53341 acattcgttt tgaaatgaga atacgattaa attgagtaaa ataaaaaata atgaggaaga
    53401 tttgcgtatt taaat
//
LOCUS       DASJTC010000008        45492 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.008, whole genome shotgun sequence.
ACCESSION   DASJTC010000008 DASJTC010000000
VERSION     DASJTC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.008"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..575
                     /locus_tag="U4B19_000450"
     CDS             <1..575
                     /locus_tag="U4B19_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2076217.1"
                     /translation="LLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGGEAVNWYNEP
                     KYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQIKHVTIPQLI
                     PLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTSTGDFGMTAA
                     AGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            587..1507
                     /locus_tag="U4B19_000451"
     CDS             587..1507
                     /locus_tag="U4B19_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2076218.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            1548..3005
                     /locus_tag="U4B19_000452"
     CDS             1548..3005
                     /locus_tag="U4B19_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2076219.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            3350..3964
                     /locus_tag="U4B19_000453"
     CDS             3350..3964
                     /locus_tag="U4B19_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN2076220.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            3957..5681
                     /locus_tag="U4B19_000454"
     CDS             3957..5681
                     /locus_tag="U4B19_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2076221.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            5678..7129
                     /locus_tag="U4B19_000455"
     CDS             5678..7129
                     /locus_tag="U4B19_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2076222.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(7602..9755)
                     /locus_tag="U4B19_000456"
     CDS             complement(7602..9755)
                     /locus_tag="U4B19_000456"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEN2076223.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(9757..10803)
                     /locus_tag="U4B19_000457"
     CDS             complement(9757..10803)
                     /locus_tag="U4B19_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2076224.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            10953..12242
                     /locus_tag="U4B19_000458"
     CDS             10953..12242
                     /locus_tag="U4B19_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEN2076225.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12255..14957
                     /locus_tag="U4B19_000459"
     CDS             12255..14957
                     /locus_tag="U4B19_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN2076226.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            14942..15799
                     /locus_tag="U4B19_000460"
     CDS             14942..15799
                     /locus_tag="U4B19_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2076227.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(15870..16769)
                     /locus_tag="U4B19_000461"
     CDS             complement(15870..16769)
                     /locus_tag="U4B19_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2076228.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            16938..19109
                     /locus_tag="U4B19_000462"
     CDS             16938..19109
                     /locus_tag="U4B19_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN2076229.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            19177..19518
                     /locus_tag="U4B19_000463"
     CDS             19177..19518
                     /locus_tag="U4B19_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2076230.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(19794..21548)
                     /locus_tag="U4B19_000464"
     CDS             complement(19794..21548)
                     /locus_tag="U4B19_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2076231.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(21559..22395)
                     /locus_tag="U4B19_000465"
     CDS             complement(21559..22395)
                     /locus_tag="U4B19_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2076232.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(22397..23080)
                     /locus_tag="U4B19_000466"
     CDS             complement(22397..23080)
                     /locus_tag="U4B19_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEN2076233.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(23239..24633)
                     /gene="uxaC"
                     /locus_tag="U4B19_000467"
     CDS             complement(23239..24633)
                     /gene="uxaC"
                     /locus_tag="U4B19_000467"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN2076234.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(24672..26171)
                     /locus_tag="U4B19_000468"
     CDS             complement(24672..26171)
                     /locus_tag="U4B19_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEN2076235.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(26164..27585)
                     /locus_tag="U4B19_000469"
     CDS             complement(26164..27585)
                     /locus_tag="U4B19_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEN2076236.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(27645..28637)
                     /locus_tag="U4B19_000470"
     CDS             complement(27645..28637)
                     /locus_tag="U4B19_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2076237.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            28869..31067
                     /locus_tag="U4B19_000471"
     CDS             28869..31067
                     /locus_tag="U4B19_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2076238.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(31525..32397)
                     /locus_tag="U4B19_000472"
     CDS             complement(31525..32397)
                     /locus_tag="U4B19_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2076239.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            32697..33671
                     /locus_tag="U4B19_000473"
     CDS             32697..33671
                     /locus_tag="U4B19_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2076240.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            33693..34385
                     /locus_tag="U4B19_000474"
     CDS             33693..34385
                     /locus_tag="U4B19_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN2076241.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            34351..35859
                     /locus_tag="U4B19_000475"
     CDS             34351..35859
                     /locus_tag="U4B19_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2076242.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            35933..37036
                     /locus_tag="U4B19_000476"
     CDS             35933..37036
                     /locus_tag="U4B19_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEN2076243.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            37039..38376
                     /locus_tag="U4B19_000477"
     CDS             37039..38376
                     /locus_tag="U4B19_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN2076244.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            38363..39241
                     /locus_tag="U4B19_000478"
     CDS             38363..39241
                     /locus_tag="U4B19_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEN2076245.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            39238..40161
                     /locus_tag="U4B19_000479"
     CDS             39238..40161
                     /locus_tag="U4B19_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEN2076246.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            40158..40568
                     /locus_tag="U4B19_000480"
     CDS             40158..40568
                     /locus_tag="U4B19_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEN2076247.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            40572..42140
                     /locus_tag="U4B19_000481"
     CDS             40572..42140
                     /locus_tag="U4B19_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN2076248.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            42380..43798
                     /locus_tag="U4B19_000482"
     CDS             42380..43798
                     /locus_tag="U4B19_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2076249.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            43810..45120
                     /locus_tag="U4B19_000483"
     CDS             43810..45120
                     /locus_tag="U4B19_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEN2076250.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT        14771 a         8013 c         9305 g        13403 t
ORIGIN      
        1 ccttactacc ttattttcta tcttgggtca ttatcagtta ctttgtttac gcttttttaa
       61 gtcctgacaa aggaatattc aatcagtgga tcactggaca tggaggagaa gctgtcaatt
      121 ggtataatga accgaaatat tggccgttta tcttggtgtt cattggtaca tggaaaggga
      181 ttggttataa cagcattatt tactttgcat ctgttatggg aatcgatccg acttattatg
      241 aagctgctat ggttgatgga gctagcaaat ggcagcagat caaacatgtg acgataccac
      301 aacttattcc actaatgacg attttaacga ttttagccgt aggaaacatt ttccgagctg
      361 attttggcct gttttataac attcctagga attccggtgc attatatgat gtgactcaag
      421 ttttggatac gtatatctac aatggcctga catccacagg agattttggt atgacggctg
      481 ctgcaggact ttaccaatca gttgtcggct ttgtcttgtt gatgatcaca aatacgatcg
      541 cccgacgttt tgatagcgat tctgcattat tctaaggagg aaaaaaatga aaaaaaagaa
      601 agtggagaga gtccaagtcc gctctttcaa taagacggct aattttttct ctaatataat
      661 gattgcactc tttgctattt cctgtatttt gccatttata tttgttatcg ccatctcttt
      721 taccgatgag tccatattgt cgactcacgg gtacagtttt tggccagaaa agttcagtat
      781 atttggttat acgtacttgt tcgaccagat gcaggataaa attttccaag cgcttttcgt
      841 cactgtactt gttactgttt taggtacact gatcaatagc acggctactt ctttatatgc
      901 ttatgcgatt tcacggtcaa actttccatt ccgtcgtttt tttaccgttt tttgtttaat
      961 tacgatgttg ttctcaccag ggatggtggc caattatctt gttatgacaa atcttcttca
     1021 gttaaaagat acgatttggg cattgatctt gccaatggct gtcagtccct tcaatattat
     1081 tgtgatgcgc acattcttta aacgttcggt ctctgattcg attattgaat cggcacggat
     1141 cgatggagct agcgaactgc gaatatttgt tcagatcgtt ttaccgttag ctgttccagg
     1201 tattgcgaca atcagtttat ttgccgcatt gggttactgg aatgactggt tcaatgcttt
     1261 actatacatc caagatgata agctagttcc actacagtat cttttgatga aaatccaaag
     1321 taatattcaa tatctgacac aaaattcagg agcaggaagc caattgacag gcggattagc
     1381 ctcagttcca ggagaatctg ccagaatggc gatcgtagtc atctcgacgt tgcctattgc
     1441 gatcagctat ccatttttcc agaagcattt tgtcaaaggt ctaacaatcg gcggagtaaa
     1501 agaataaaga aacaaaaata taaatcataa aattaggagg aagaaaaatg aagttatgga
     1561 agaaactagc ctttacaggt gtttcagcgt tgttgttggg aacattagca gcatgcggag
     1621 gttctggttc taaagaccag gcagaagcta gcaattcgga cactttgcag atgtatcaaa
     1681 tcggagataa accagataat ttcgatcaat tgatggaagt agcaaacaaa cgaatcgaaa
     1741 aagagatcgg tgtaaaagtc aatatcaatt acattggctg gggcgattac gaaaagaaaa
     1801 tgaacgtcat catttcttcc ggggaaaatt atgatatcgc ctttgcgaac aactatgttt
     1861 ccaatgccca aaaaggagcc tttgctgatt tgactgaatt agcgcctaag tacgctgaaa
     1921 aagcttataa tgatttagat gaagcatata ttaaaggaaa tctggtcaat ggaaaattat
     1981 atgctttccc agtcaatgga aatgtctttg ctcaacaagt acttactttt aacaaagctt
     2041 tactagataa atacgatctt tcaattgatg gaatccaatc ttacgctgat gcagaaaaag
     2101 tcttgaagga attccatgaa aaagaaccaa acacggcagc ttttgccatt ggacaaggct
     2161 tcaaagtcca agggaacttt gattatcctt tagggaatac gttaccattt gctattgatc
     2221 tggatggaga tgattcgaaa atcatcaacc aatacgataa tgaaacgtat atcggactgt
     2281 tacgtacaat gcatagctgg tacaaacaag gactcattcc aagtgacgct gcaacgagca
     2341 ataaggatta tccactagaa ggaaatactt ggctaatgcg tggagaaaca caaggaccgt
     2401 atgattatgg cgatacaatt ttgacaaatg cagcaaaaca agaacttgtc tcaaaagcaa
     2461 ttactgttcc actaaaatct acagctcaag cacaaatggc aaattttgtc gtatcaaata
     2521 cttcaaaaaa taaagaaaaa tcagtagaac ttctagggct gcttaacagt gatccagaat
     2581 tattgaatgg gcttgtttgg gggatcgagg gagaagcatg ggaaaaagtc gatggttcag
     2641 atgataaaat caaattgcta gatggttatc agccaaatac acatatgtct gcttggaata
     2701 caggaaacaa caagattcta tatacacaag agtctattac ggatgacatg attgccaaac
     2761 gtgatcaatc gattgctgat gcagaaactt caccgatttt aggattcagc ttcaacactg
     2821 acagtgtcaa aacagaatta agtaatattt ctaatgtcat gaatcaatat ttagatggat
     2881 tgaatacagg gactgttgat ccagatgaaa cattaccgaa attgaaagac gctttggaca
     2941 aggctggcta tgataaggta ttgaaagaaa tgcaaaaaca atatgatgag ttccgacaag
     3001 aataataatg aataaagaca aaggggttgt gacaaaagac gtgtcacaac ctcttgttcc
     3061 gaataaacgg tgttcaaaag ccagtttctc ggtattaagc taaatatatg taaaaatgtg
     3121 gaaagccata ttcacaaaat ttttcttact actcgaaact aagcggctat ttcacaaact
     3181 cttggtaaac ggtgttcaat cagctaaccc tcggtattcg ctcacaaaac gacaaaatat
     3241 gaggaactat ttttccgttt gtttctctaa taatcaggtc aaggtgctga ttgcccaacc
     3301 tttttttgta taatgagaaa gtataaactt aatgtttgga ggagaagata tgttagggaa
     3361 ggttttggaa gagttattta tccggatctg ggtcattata aaactaacct tgtatttctg
     3421 gatatataca tttgccggcg ggatcatctt tggattaggt gcggcatgga aaacagtcaa
     3481 tgaactattt tatttgtacg gattcgagta taaagaaatc acaataaaaa gaggctggaa
     3541 tatatataaa agaaattttt taagaggcaa tctgctattt agtttatttt tatctggtac
     3601 tgctttattg agttacaatt tatatttatc tgttcaaatc aaagggctca tttttttagc
     3661 tattgatttt attttacttt ttgccttgct ctgcctattt gctacttatc aatacagtat
     3721 gattttagat agtgaatata cgatctctgt tcctaatctg ctaaagctaa gttttatttc
     3781 ggttttttcc agcttttcgt cttttctaaa aataattatt ggtagcggta taattttagg
     3841 cgtgacttgg caattcaaag gattgatcct gtttggtgtc attggtcttt tgacggtttg
     3901 gaatggtacg atgacaacac attggcgtga agaattagat aaacagttgg aaagttatga
     3961 ataagtttcg gattctattt tcaaaaggaa ccttactaaa tcgtctgttg cggaaatacg
     4021 cggttatcat gatcggtgta acgatgactg ctaccgttat ttttagtatc catacttggg
     4081 aacaaaatca aaaacaggca gaaaacatga caagtgatgc cgttcaaagc acaagcagaa
     4141 tgctgaatga taaaacaacc ttgagtagga tcatcaaaaa ccagcttgta gggaattccg
     4201 aaaagatcga aaatgtgacg acctacttaa caaaacccat agatcagtat ctgatgtatg
     4261 tatacgaaca gcaaaacagt accgatgaat tagtttcttt cccaaatcaa atcaaagatc
     4321 tctacgcaaa ttatgaagaa ctaagtgcga tatatatcgt gttgaatcaa ctaccggagt
     4381 attatgaatc ttctagagaa aacaaaggtg gggaaataaa aaaggggaca ccaaaattac
     4441 tagacgcctt ttatatcaag ttgcctatca cacaaggagg agctgagtca ggaagtattt
     4501 ttattggttt tcaaaagaaa gaactagata tgattttaga aaatctaacg tcattcaatg
     4561 gactctcttt atacatgatt tctggaacga caaatcgact atacacattc catgataata
     4621 aaatagctaa agaaacgttt gaaagacaag aaatgatcat cacagagagt ctgaagaaaa
     4681 attcacaatt gccgattgac gatctggaaa aaaacaattt tgttcaacac caagaactgt
     4741 ctgatgatta tcgtatatta gctgtactag ataaaaaaag tgtaaaaatc gaaacgggaa
     4801 aaaatcttgc cccgctgatc attggtatca ttattttaga caatatactg cttatctttc
     4861 tttataaaac attcaaacga tattcacagc aagtatctat cgtcatgcag gccatggatc
     4921 aaacagcagc agggaattta gagacgcgga tcgacataac aaacacggaa tatgaactga
     4981 aggaactttc aatcggtatc aacgaaatgt tggatagcat caatcagttt gtagaagaca
     5041 tctacaaatt agaaatcaaa cagcaagacg cacatatgcg ggcgcttcaa gcacagatca
     5101 atccacactt tttatataat acattagaat atatccgaat gtatgcaatc agcgaaggaa
     5161 gtgaagaatt agcagatgtt gtgtatgcgt tttccgcttt gcttcgaaac aatacgaatc
     5221 aagagaaaac gatcacttta aaagaagaac tggacttttg cgaaaaatat gtctatttgt
     5281 atcaaatgcg ttatcctaat cgagtagcct atcattttat gatcgatcct gatttggaaa
     5341 agatcgaagt acctaaattt gtgatccagc cattagttga aaattatttt aaacacggta
     5401 ttgattttac tcgattcgat aatgcgctaa gtgtcaaagt cttgcaggaa ggcaaacggg
     5461 taaggatcat catcaaagat aatggaaaag ggatgactga aaaacggcta aaacagattg
     5521 aagaaaaact gtctcatcct aaagtggaac ttcacggttc tatcggtctg caaaacgtga
     5581 atgaacgttt acgtgccagc ttcggcagca gttattatat gtcgcttgag aataatgaga
     5641 caggtggact gaccgtttct attacattca aggagggatg agcgtgtatc aagtgttatt
     5701 ggtagatgat gaatacatga ttttgaatgg gttaaaaaag ataattgatt ggcaaagttt
     5761 aggctttcaa atagtagcaa cagcagagaa tgccaaagaa ggactagctg tacttgaaca
     5821 gcgacagatt gatttagtag taacggatgt gacaatgcca gaaataaatg gactggaatt
     5881 tattgaagcg gctcaaaaag aacgtcataa ttttgaattt atgattttat caggatacca
     5941 agaattcgat tatttgaaag gaggtatgca attaggcgcc gtgaattatt tgatgaaacc
     6001 cgtgaataag ttcgaattgg ttgaaagcct caagaaaatc aaaacacggt tagatcagca
     6061 aaatgaacaa aaaaatcaac aagaaatcta tcaagaactg ttattcagtc aatggttgaa
     6121 tgaagaattg gatgaaataa gtgaagaaga aataatagga caaataagtg agaagcagca
     6181 taggatagta ttgattcaac tggcaagaac tcatgatact cttatcaata gatggttgca
     6241 agaacggcaa caaatctttt actatcaacg aaattacgga gaccgtgtat tgatcacgtt
     6301 gttgttggag gcagatgaag ttgatggctt ctgttgtttt gttcaagaaa actttcctga
     6361 tcaggaatgg ctgatcagta tcggggaaga gacagattct ttggacaaaa tccctgaaac
     6421 attccaacaa gcgaaagaca atctacagtt gcatcaattt tatggagata aagacgagca
     6481 tgttttttac gcagtacaag cagaaacaaa agatcaaacc atcgattttt caagtttcag
     6541 acgcgtgctg caaaataaac aattggaagt cgctcaaaaa atgatcactg atttttttga
     6601 acagtttcag ttagcagcca tgatgccgga agatatacga tattccagtt ttttactgtt
     6661 tatggagatt caacgggaat tgattgatct ggaagatgag gaatatttac aaggaatcga
     6721 aaaaatccat caggcaaaaa cggtgcaaga acttcagcag ctacttctgt cattcatcaa
     6781 aaagcatcag agacagaaaa agtacagtga caatgtggaa aaagtaatcg taatcctgca
     6841 ccagcattat caagaaccac ttacactcaa agaagtgtca gaaaacctgc atttaaatgt
     6901 catgtatcta ggacagctgt tcaaaaaaga aacgaaaaaa agtttttcag cttatttgaa
     6961 ccatttacgt atggaaaaag cgaaacaact gcttttgcac agtaatcaaa acatcaatga
     7021 gatagcaagt gagattggct acaacaacac cacgtatttc tctaaattat tcaagaaaat
     7081 cgtcggcaga tcccctaaag agtaccgaga aaacagtgga gaatcataaa aaaaaaagcc
     7141 aatgtaatga aataacttat aaaaaattgt ctagaaatct aattttgggc tgttcttatt
     7201 acttgaggca aggcagcttt ttcatgacct ctcgataaac ggagttcaaa agccagtttc
     7261 tcggtattaa gccaaattta tgcaaaaatg tggaaagcca ttttcacaaa atttttctta
     7321 atactcgaaa ctaaacagct ttttcacgac ctctccacta aatggtgttc aatcagctga
     7381 ccctcggtat tagctcaaaa aatggcaaaa tatgaggaac tattttgcca tttgtctctc
     7441 taatactcag gtcagaacgc tgatttcaca acctctcaat aaacggtgtt caaaagcttg
     7501 catctgcggt aataagacca accaaacgaa aaatatgagg atctattttt gcttggctgt
     7561 tcttattact tgatgcagga cagctttttc acaacctctt gttaattata gtttgataag
     7621 gaaaaaggca aatcttttgc ttgatatttc ttagggtgag ggatgatccc taattggtaa
     7681 gagatcgtgc cgcctttcag caaatcttga tgggtaaaat ataatttcgt atattctctg
     7741 tccatccgtt tgattttatg gataaattgt tgctgcggcc gattaggcgt tgcttcaatc
     7801 gtcaattcct ttccatttga caaaagcaaa acagccttgt caattaatgg cataccgata
     7861 acatattcac cggttccagg cgtgacagga taaaatccca gactattgaa gatataccaa
     7921 gcggccatac ttccattgtc ctcatctcca gggtaaccca ttggagaatc gtcgaaggtc
     7981 tgagtcatca actgtttaag cagtggctgt gccatttcag gttgtcctat ataactgaac
     8041 aagtaaggat agtgaaaact tggctggttt gaaatagcta attgtccaaa ttctatcgcg
     8101 gccatctcac tcatttcgtg gatctcaaaa ccatatcctc ctgggtcaaa cgttgggggc
     8161 tgattgcaca gctcaatgag tttttcttta tatctctcac tgccaccata ttttccgatc
     8221 agtcctgaaa aatcgtgata aacagcaaag ctgctttgcc aagcacttcc ttctgcataa
     8281 tcttgtcccc aacgattagc tgaaaatggt tctctgaact tgccatcttt atcttttgaa
     8341 cgcataaatc ctgactcata gtcaaagata ttttgatagt tcaatgattg ctgctgatat
     8401 ttcttcgtca tttcctcttc ttctagtacc ttggcaactt ggctaataca aaaatcgcta
     8461 taagcatagt ctaatgtgtg attgactgat tcgtggtagt gattaggaac atagccatat
     8521 gtcaagtaat cttctgttcc ttggcgtcca taatttggat gtttgctttg aaccgtagca
     8581 cctttttcca ttgccgttag aaattcaggc atcaagtctc gtcggatatc ttttacagca
     8641 gcatctgcaa tgaccgcatc gatcaatgtc ccaggcataa gtccacgttc atcaggagac
     8701 agccattttg gcaaatagcc actttctcta tagctgttca aaaaaccttc cagcatttct
     8761 tcgtattttt ctacagcaat caaagaataa agaggataaa ccgttttata cgtatcccaa
     8821 aacccattat ttgtatagag aacaccaggt tttatagatc gactagtcgt atcatagtgg
     8881 attttttgcc cactaggatc aatttcataa aatgtttgtg gaaataaaaa cacccgatat
     8941 aaattatgat aaaacgtccg caattgttgt tgatcatggt gtgtgagctg tatacgatca
     9001 aaataggact gccattgttc ttgactgtga gcaaggtaag tctctttgct ttggttttgt
     9061 tctagttcca ggttataagc tgcttgttcc gagctgataa aagatgtccc aaatcggata
     9121 gtttgttttt ttgtctgttc aaaatacaac gggatcgagt cgctattttt gttggaaaca
     9181 gctaactcat caattgttaa aggatattca aatctcaata taaaataaaa agaaaaatca
     9241 ggatcttctg aaccagcaaa atgtgtgatt tttccttcca ctgtgtaagg atctataaca
     9301 aagagctgat ggcgtcccgg aaaggataaa agtaaaccgc tgtcattttt gctgtaatca
     9361 atagataaga ttccgccata catgctaggt atcaatgtag aacagatgcc ataacgtaat
     9421 tgacagatcg acagatgagt cgggcaaaat atactttctt ctgggcggta agaactttga
     9481 gcatggaaaa gggtgttctc ctgtagtttt ccattgatcg gcgtaaacag catatgactg
     9541 aaatcaccca tccaaggact tggttgatgg gtaagacggt agccttgaaa aacgcggtca
     9601 tcaggatgaa accaccagct gccgttttga tccgtagttt gcggtgcaaa ataattcatc
     9661 ccaaaaggaa cgccagtata aggctggcag ttcccattag agaaatttgg ctgattcgct
     9721 gttgcatgtc tagtatctat ttgaccgata ttcattttat ttctcctttg ttgattgtct
     9781 tttgatgaat gaaactggaa atttactatc ggatggtcga tcttttgtct ctaaccaagt
     9841 gaccaaagca tctcccgcaa gcttacctaa ctgttcaaaa tcttgtgcga tggttgtcag
     9901 tggcgggtca acaagagctg ctgcttggat atcatcaaat ccgataattg aaaaatcttc
     9961 agggatatga taaccatgct gtctcaagag attcatggtc tggatagcga caacatcgtt
    10021 ttcacaaaca aaagcagtta cttcatactt agtgatataa gaaagcaatt catctaatgt
    10081 cgcgcttttg tcatccagtg aagtgtggaa agtcaaacca gctttgtcca atgcttcaag
    10141 gtaaccaagg taacgctgtc gaacggattg tggatgagtc tgcgtaccaa acaaataagc
    10201 gatttttgaa tgtccctgtt cgatcaaata agaggcagca agcgcaccgc cttggaaatt
    10261 atcggaaaga acggtaggaa aaggtaactc atagattttt ttatctaata tgacgacagg
    10321 gaattctttc aatgaaagct caaaaagtaa gtcaagatgt tgttctgtat caagagcata
    10381 ataaatcaat ccgtcaaatt cattgattag ctcttttgct gttttatttt gtagaaagtc
    10441 aagcgtcgtc atcatgacat caatctgttt atcttgcatc acagggttca atcccttgct
    10501 aaaatcacca acagacaaat cagcgacata tggaagaaga aacagaatcc gtcgtgtttt
    10561 tgtaggagaa gcaagaatct gttccggaga agcggattta acgaaactgc ctttcccgcg
    10621 aacgcgataa ataagtccta attgctctaa ttcagtcaat gcacgtttgg aagtgattct
    10681 gcttacttta taggtacgtg aaagttcttt ttcagttggt agttggctgt ctacaggtaa
    10741 ttcaccagtt aaaatagccg tttgcaaatc attaaatatt gtctggtaca atggttgagt
    10801 catataaaca cctcttaatg atatatcata ttcaaataat accataatgg atgaaaaagt
    10861 aaataaaaag atattttttg aaagcgtaga cataattggc tgtatgaagt ataatcttta
    10921 aagatatatc aaatagaaag aaggttttat tagtggttta taaagatata ccaaaatcgg
    10981 tgcagaaatt tatggaagat atcacacaaa aatgtgggga tatccatgcg gactgggcaa
    11041 agaattttac agcagcattt gccaatactc tgttgacaac agtcaaaaag caaaatgacg
    11101 gaaccacctt tttattgacg ggagacattc cagcgatgtg gttaagagac tctactgcac
    11161 aagttcgtcc ttatttagtg attgcaaaag aagatgagga attagctgac atgatcagcg
    11221 ggttagtcaa aaagcaattt ttttatatca atatcgatcc atatgcgaat gccttcaacg
    11281 aagaagctaa cggtgctggc caccaaacag atcatacaaa aatgaacgat tggatctggg
    11341 aaagaaaata tgaaattgat tctttatgct acccaataca gttggcctat cttctttaca
    11401 aaaatacagg aaaaactgat caattcaatc aatcttttat tgaaggtatc aaaaaaatct
    11461 tggacgtgtt caaaacagag caagatcatg aacactctcc ttatacattt gtacgagaca
    11521 cgacaagaaa agaagatacg ctgatcaatg acgggcgagg tgcaaaaaca gcttttacag
    11581 gaatgacatg gtctggtttc cgtcctagcg atgatgcttg ccgttatgga tatctggtac
    11641 catccaatat gtttgcggtg gtcgtgttag gttatgtgga agaaattttt caaacgatac
    11701 tcgatgataa agaaattgta cagcaaacac agaaattgaa gaaagagatc caagagggga
    11761 ttcagaatca cggacgaacg gttaatcaaa aagggcaaac gatttatgca tatgaagtag
    11821 atggaatggg aaacgccaca gtcatggatg acagtaatgt gcccaatctg atcgccgcac
    11881 cttacttggg atattgttct gaacaagagg aagattatct gcaaacaaga agaacattgt
    11941 tgagtaaaga aaatccatat ttctatgaag gaaagtacgc aaaaggaatt ggatcctccc
    12001 atacgccaga aaaatacgtt tggccgattg cattagctat ggaaggaatg acaacgtcag
    12061 acaaatctga aaaagagcgg atattgaatt tacttgtagc aactgacgca ggaacacacc
    12121 ttatgcacga aggattcgat gtgaacaatc ctgaaaatta tacgagagaa tggttttctt
    12181 gggcaaatat gatgttttgc gaattagtca tggattactt tgatattaga gtagaaaagt
    12241 aggaggtatc gtaaatgaaa aagaaagtat atatcatttc tcatagccac tgggatcgtg
    12301 aatggtacat ggcttacgaa cagcatcaca tgcgactgat tgaattgata gacgatctac
    12361 tagagctatt tgaagttgat ccgtccttca acagttttca tttggatggg caaacgatta
    12421 ttttagatga ctatcttcaa gtaaggccag aaaaaagaca ggcaatccaa caagcaatca
    12481 atgaaggaaa attgagaatt ggaccatttt atatcttgca agatgatttt cttatcagct
    12541 cggaatcgaa cgtgagaaat atgctgattg gcatggaaga aagccgtaaa tggggaaccc
    12601 ctgtcatgtt aggctatttt cccgatacat ttggaaatat gggacagacg cctcaattaa
    12661 tgaaacaagc tggtatttca gcagcagcgt ttggccgtgg tgtaaaacca attggctttg
    12721 ataatcaagt tttggaagca gaaaactatt cttcccaata ctcagagatg tggtggaaag
    12781 gccctgatca aacagcaatc tttggtctgc tatttgccaa ttggtatagc aacgggaatg
    12841 agattccagt agaaaaagaa gcagctcttg ccttttggaa gcaaaaatta gcggatgcag
    12901 aacaatatgc ctctacgaac cacttattga tgatgaatgg tgttgatcat caaccggtac
    12961 aaaaagatat atcaaaagca atccatctcg caaatgaact gtttccagat tacgagttta
    13021 ttcattctaa ttttactgac tatctggaag cagttcaaaa agatgtgcca gaagacttag
    13081 gctcagtaga aggagaattg acaagtcaag aaacggatgg ctggtatact ttagccaata
    13141 cagcatctgc tcgggtttac ttaaaacaat ggaataccaa ggtccagcga cagctagaaa
    13201 atattacaga accacttgcg acgatggctt atgaagttag tggaaactat ccccatgatc
    13261 aattagatta tgcttggaag acattaatgc aaaatcatcc tcacgacagt atttgcggtt
    13321 gttcagtcga ttctgtccat cgggaaatga tacctcgttt tgaaaaagca gatgaagttg
    13381 gaaagtatct tgcacaagat tccttagaac agctgaccgc agcaatcgat acgaccggat
    13441 tcccaaaaga cagctttcca tttgtcattg taaatactgc aggaatggat aaaactggtg
    13501 aagcagaaat aaccatcgaa ttagaaagaa aaagattcgc tgaaggaata cctgaacaac
    13561 tatatcaaga gttagaaaat ttgcctaaac gtaagtatca tgtagaaaca aaaagtgggg
    13621 ccactatacc agctatttta tctgaagaaa ctgtccaatt tggttatgat ttgccgaaag
    13681 accgtttccg tgtgccatat atggcaagaa tgatcaaagt gactttacca ttagaaaaca
    13741 tgccagcatt ttcttgggaa acattcgcgc ttgtagaagg ggaagcgaaa gcagaagaaa
    13801 aggaaacaat gattcatcag agtggacgaa tcatcgaaaa cggccctcta catttgacga
    13861 ttgaaaaaaa tggtaccatt acaatggaag atcgcaaaaa caaacgaaag ttgaataatc
    13921 tgcatatttt tgaagatatc ggcgatatag gaaatgagta tatctttaaa caacctttct
    13981 gtgatcagcc aatcctatcc agtaacaaag aaaactctga agtaaaagta ttagtagata
    14041 caccggaaat agcaaaaata agtattctac aggaaatgga aatccctgtt tctgcagacg
    14101 agcgtttaga aaaagaacag cagatggttg tggaatttcg ttatcgaaaa gcagaacgat
    14161 ccaaagaaaa acgaatcctt caaataaaaa caatcatgac aatcagaaaa gatagtaaaa
    14221 aaatcgactt tgaaacgact ctcgacaatc agatgaaaga tcatcgctta cgtgtcttgt
    14281 ttcctacaaa gttacatgtg gaacatcacg aagcagatag tatttttgaa gtggtgaagc
    14341 gcccaaacca tgtgtctaaa agttgggaaa accccacgaa tccgcaacat cagcaagcat
    14401 ttgtcaatat tcatgatgaa gagtacggtg taacagtcgg aaactttggt ttgaatgaat
    14461 atgaagtaac agaagacgga caaatcgcag tgacgctact tcgcagtgtg ggagaactag
    14521 gcgattgggg atattttcca acaccagaag cacagtgtct aggtgagcat cggttcaatt
    14581 acagcattga actacatgga ccagaagaaa aattctctac ttatttgcat gcatacgcag
    14641 cacagattcc tttttccact caacaaatca aacatcatga aggaacattg atttcaaaac
    14701 aacaatattt aacgataaaa agtgaaacgt ttgcaattac agctttgaaa cgcagcaagt
    14761 ttagtgataa agtagtagta agaggtttca atatgtctag tcatttagaa aaattggaaa
    14821 taactaaaga caatggaaaa actgtcatat tgaatctgct tgaagaacca acgaagcaag
    14881 cggttgtccc aataatccaa ccttatgaga ttcggacaat cggattcgag gaggaaaacg
    14941 aatgtatggc ggaatagaag caggcggaac aaaatttgtg tgtgcggttt cagatggtgg
    15001 agaaatttta gaaaaattaa gtatccctac cacaacacca gaagaaacat tggatcaggt
    15061 atttgaattt tttgatcgtt atgaattgaa gggaatcggg attggctcat tcggcccgat
    15121 tggtatcgac aaaacaaaag ataattacgg atacgttttg gcaactccca aaaaagggtg
    15181 ggcacaattc gattttcttg gcacaatcaa ggaacgctac aatattcctg ctgcttggac
    15241 aacggatgtc aatgctgctg catatgggga attgaagcaa ggagcagcac aaggaaaaaa
    15301 tagttgtatt taccttaccg tgggaacagg aatcggtgca ggtgtcgtga tcaatgaaga
    15361 aattttttca gggatcgcac atcctgaaat gggacatatt tgggtgaaac gccatccaga
    15421 tgaccattat gaaggtactt gtccttatca taaagattgt ttagaaggat tagcagcagg
    15481 accgtctatt gaggcacgca cagggatcaa agggcaagat ctgccgcaag atcatcctgt
    15541 ttgggctatt caggcttatt atattgctca agcactgatc aactatacgt tgacgttagc
    15601 acctgaaaag attattttag gcggtggggt aatgaatcag gatcatctcc ttcaaaaaat
    15661 ccgtcagcaa tttgttgaat tgatgggtgg ttatatggaa acacctcaag taagtgaata
    15721 tatcgttcgt tggggactgc cgaatgaaag tgggattatc ggtagtttgc tgttagcaga
    15781 acacgaacat caaaagtaag cagacaaatt acacaaaaag aacagagtct tatcattatc
    15841 tgctgataag actctgttct tttttatttc tattcccata atccaggtaa agctttaccg
    15901 actatttttt cttgagtaaa tggatggatc atgtgcagtt ctaatgcatg gagcatcagt
    15961 cgttcagctg ttctattttg gtagagaggg tctccaacga tcggatgtcc cttatgagct
    16021 aaatgaactc tgatctggtg tgtacgtccg gtttccaaaa cacaatagac ttgtgtttgt
    16081 tcagaaagta ctttgttgac agtgacatgt gtcaaggcgc tctgaccatt tttatcatct
    16141 acgacccttt ttctccgatc atggcgatct cgtccaattt tattggtgat ggtcatatct
    16201 ttggcaattt ctccccatac gatggcttga tatctccgat agatcatttt ttgttccaac
    16261 attcttccaa gtatcggcaa gacaaatgga ttttttgcaa ataaaacggc accgcttgtt
    16321 tctttgtcca agcggtggac aacgtaaggt cgttgctgtt tagtgtcgag ataagcagct
    16381 aaatgattga gtaaggtact gttttctgtc ggttcatttg gatgagtctt cattcctgca
    16441 ggcttatcca ctataatcaa atgctcatct tcatataaaa cacggatgtg cttcgcatct
    16501 cctaattgaa cttcttgata attataatct gtttcttcga ataacaatgt gatctggtca
    16561 ttgcccttta cttcatgatg gaacaaagca ggttgttgat tgatccaaac gtttttacgt
    16621 atacgcaaaa agtgtcgaac ctttctagga actaaccatt ccttttcaag caagtcgcga
    16681 attgttgttt caggatggtt cataggtaaa gtgatcgtga ttttcacaag tgtactcctt
    16741 tcagtgcaat cgcagactaa ttgtaccatt tttaaaacat atttaagaaa aaacttacat
    16801 ttttctgttc attcttatgt tactctactt gcactaacgt aaaaaaacat attgaactac
    16861 aacttaagca ggaagaaaac actctttcac tatggtaaaa taagacgatt gaatcaaata
    16921 gacaggagaa gaagcaaatg gattttcaaa gcattttaga aaagataaaa gctgctttga
    16981 tcagtttttg gcgttggatc agaccatatc tcggacaatt ccatcgatgg cgaaagcgca
    17041 tctggaaaaa atatcatgtg aacaaattga ttttgctgat tggtttgatc tgtgtcttag
    17101 tgacaagtat ctacctcttt attttagcaa aacaagcaaa tgtcgaaacg ttaaaatcag
    17161 gattaagcca atcgacagtc atttatgata agaaaaacga agaagctggc acactgtacg
    17221 cccaaaaagg aacatatgta gaattaaacg cgatttctcc ctatatccaa gatgcagtaa
    17281 tctcgacaga agataggaat ttttacgagc atcacgggtt tgatatcaaa gggattgccc
    17341 gtgcagcagt ccgaatggtc ttgtctggcg gaacatcagg gggcggagga agtacgatca
    17401 ctcagcagct tgccaaaaat gcgtatctga cattagacca aacgtttgat cgaaaagcga
    17461 aggaattatt tttggcaatc gagatcgaaa agaagtacag caaagaagaa atcttgacca
    17521 tgtatcttaa caatgcctat tttggcaacg gggtatgggg tgtccaagac gcttccagaa
    17581 agtattttgg agtagatgca acgaatgtta cgctgtctga ggctgctact ttggcaggaa
    17641 tgctgaaagg tcctggtatt tataatccga tcgatcaccc aaaaaatgcg aatgatcgga
    17701 aaaacacagt actaagtgta atggaagaaa acggaaaaat cacaaaagaa caagaacaag
    17761 cagaaagcca gacagatatc agcgcgtatc ttaatgacac atacgataat tcagattctg
    17821 gttatcgtta tccttattat tttgatgcag tgatcgatga agcagttaac gagtatggtc
    17881 ttgatgaaga agatgtcatg aataaaggct ataaaatcta tacagcttta gatcaggatt
    17941 atcaaaagca attagaagct acttatcaaa acagtgcatt attcccagca aatgcttcag
    18001 atggtgccat ggtccaatct gcttcagtag ctttagatcc gaatacaggt ggtgtacaag
    18061 cactagtagg acgaagagga gatcatgtct ttagaggatt cagctttgct acacagatgc
    18121 gtcgttcacc aggatcaacg atcaaaccat taagcgtata tacaccagca ctggaagctg
    18181 gatacaagcc ttccagtatc ttgaaagacg aaccgcaaag ttattacgat gcgcataatt
    18241 ttgacgggac ataccaagga gaagtaccga tgtatcaagc tgttgcacaa agtttgaatt
    18301 tgcctactgt atggcttttg catgagatcg gtttacaaaa aggatatgat aaagcagaag
    18361 aattcggcct gcctttagca aaatctgata agtattatgg cttagccctt ggtggactgg
    18421 aaaaaggagt atctccattg atcatggcgg gtgcttacag tgcatttgcc aatgatggtc
    18481 agatgtatac gccgcatctt atcacgaaaa tcgttgattc gactggagcg gtgatcgtcg
    18541 ataacacaaa caaaaaacca aaacaggtca tatcaaagga aacagccgat gaaatgacca
    18601 gcatgcttct tggtacattt tcaaatggaa cagcggctgc tgcgaatcct gcaggttata
    18661 cgatagcagg gaaaacagga accacagaaa ctaatttcga tgccacaaaa gcaaatgacc
    18721 aatggatgat cggttacacg ccagacgtag tgatttcgac atggatgggt tacgaagaat
    18781 ccagcaagct gcactatttg gaagggacaa gtggaaatgt tgtcgggaaa gtcttcaaat
    18841 cagcggcaga aggcatcctt ccgtatactt caaaaacagg atttagtgta gcagatgcct
    18901 atgccacagg aggacaagtg gtagctgcag atcaagttgg aaacacagga aattctaatg
    18961 gagagtctgg aaattggcaa gataacttga atcaatatgg tgaacaagca aaagaagggc
    19021 tgaaaaactt cggcgatatg gtgaaagaag gcgttcaggg aattggtgaa gcggctaaag
    19081 acctctggcg gaaatatcaa ggagagtagc ccctttgaaa acgaataacc ccatgttaca
    19141 atagagagta actactaaaa gaaaagaggg cataacatgt caaacatcta cgatagcgcg
    19201 aatcaaatcg aacgtgagat ccgtgaatta ccagaattca aagcgttgaa agaagcgtac
    19261 gagaaagtac aagcaaacga agaagcacac aaactgttca aagacttcca agcgatgcaa
    19321 atcgaattac aagaaaaaca aatgagtgga caagagttca gcgacgaaga tgcaatgaaa
    19381 gcacaagaaa tggctgtgaa aatccaatca gaagaagtaa tcaatgactt gatgcaaaaa
    19441 gagcaaggct tcagtacaat catcaatgac ttgaaccgta tcatcatgac accagttcgc
    19501 gacctttaca gcgaataaaa ctattttatg aaacaacaga gggtgtgaaa gagacgttcg
    19561 gccttgagcg atgacaaaat tgcttcttat tgccgatagg ctgtctcttg aacaccattt
    19621 accaagaggt cgtgagccta ccgtttaact ctgagttaca aggaacaatt tcaaaaaaca
    19681 gcttctcatg tttgatgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
    19741 ttattcagaa ataggagcta cgacaaaagt cttgtcatag ctcctatttt ttgtcattca
    19801 tttttcaagc ggtagaaata gcctttttga gctaacagtt tttcgtaagt acctatttca
    19861 acgatttttc cttcatcgat cacaacgatt ttatctgcct ctttgatcgt tgaaagccga
    19921 tgagccacaa taaaagtagt gggtgtttct gtcaggtaat tcagggcttg ctggattttt
    19981 ttctctgact ggttatctaa agctgaggtt gcttcatcta gcaaaatgat ttttggctga
    20041 cgtatcaacg ctcgtgcaat ggagatacgt tgcttttggc caccagacag cttgttgcca
    20101 tgttcgccta ctaaggtatc cagaccatca ggaagctggt caatcagtga agaaagttgc
    20161 gcagcttcga tgacttcatc taaacgttct tttgatacgt ttttcaagcc ataagtgatg
    20221 ttctctttga tcgaagcgga gaacaacaaa gtggtttgag gaacaacggc taaataattt
    20281 cgcacacttc gcatatccag ttcatccaaa ggctgatcat ctagaaaaat ttttcctgaa
    20341 gtcggctgga taaatccaat caataagtta aggagtgtag attttccaga gccagatggc
    20401 ccaacaaaag caattgtttc tccagctttc acctctagag aaaaatgatc gataatcggt
    20461 ttgagtgagt ccggataaag aaaagaaaca tcctcaaaac gaaaatttcc atccaaatgt
    20521 tggatttttt tcttaccgtg ataagtttct acatcgccag caatcaacag ttcgccaata
    20581 gaagtgatag attccatgcc ttttgaaatc gtaggaatca gcgtgatcat tccagaaacg
    20641 gaattgacga tcgtcgtgaa atacgtctga tacatcacga catcgccggg ttggatgcgc
    20701 ccttgtaaag ctaatatccc agtgaaaatc aaacaaatca gctgaaatat ctgaaaacta
    20761 gcccagctga ctgatccgaa aacagattgc aggatatcta agtggaatcc tttttcatag
    20821 atttgagtaa aatgcttatt taagcgagtt gtttcgtgtt tttctaaccc atgagctcga
    20881 gcaatcggtg ctaattcgat cacttcgatc acttttgcag aagtctcttc catttcttgc
    20941 ctgaagtctg agttggactg tctgatcctt ttacggaaaa agagagtaac gaatgcggcg
    21001 agaggaacag tcaataagaa aaacatgaaa acaacaggac ttttataaag cgtgatacct
    21061 aaagctacga ataaattgac agcgatattt aatccgctga caaatacttg agaggacaac
    21121 gtttggatag cttctacatc acggatgatc tttgactgga ttcgtccaga ttgcaattct
    21181 ttttgataag gaatagatag ttcttggatt ttcttgataa gggtcgctct tagtccagct
    21241 tctacttgac ggatcgaagt gctatggtat tttacatgaa aataattcgt caatatattt
    21301 tgaccaatca gacaagccat caaaaaagcg ttgaaataaa tggcctgcaa ctgagaagaa
    21361 tctttcgatg aagctgcatt gattacattg gcaatagcga tcggcattaa ccaagatgga
    21421 agatgtttga taataaaaaa gagtcctgac atggcaaaac gccaataatt tccctgataa
    21481 agatgcagca atgtctgaaa agcatttgca tgatattttt gataactttt ctgtattcgt
    21541 tcctccataa tcttctcctt aacaatcttg gtaaataaac tgcaagtgtt ctattgcttc
    21601 aggaacataa atctcaggag tagcttcaag agtggcgtac attagaggac gctcatattt
    21661 gatataatgc agtaccgggc ggaaatctaa acagcccttg ccaacaggta ccatgcggat
    21721 ctttccattt tccatgatat agtctttgag atgaagcgct gtaatataac catgtaaatt
    21781 gtccaaggca cctcggatca ctttttcctg atcagcatga ttttctttat gcatcaaatt
    21841 cgcacaatct aaaatgattt ttaaatttgg tgaagctact tcatcgatta aacgtttagc
    21901 taactgatag ttatagattg gatgattgat ccctgcttca acagcaacag ttacgccaaa
    21961 attttctgca tgagcgacta attcaatgat agaatttctg gcgatctgat aagcttcttc
    22021 agtaaaattt tcttttgtgt aaccgacaga gacactaccg gtttccgtag caatcatctt
    22081 ggcttgatag gcagagcaca gagcaatttg ctgtttgaaa gttgccaatg cttgtgcacg
    22141 aacttcttga tttttgctgg ataggttgat atagcatcct aaaatggaaa gttcgatatt
    22201 gtttttttgg aagtattcac caaaataggc agcactccca agtgtcatgt cagttaattt
    22261 aagttgttca ggaaatgctt tttgtggtgc aaattggata tgggacaagt gaagctgtga
    22321 tactttttga acgatttctt cacgatttcg aacatctaaa tcgtgggcac ggatgcctaa
    22381 atttaatgga aacatctcaa actccttttt gtaaataatt agcaagaggc agacgggatt
    22441 cttttatcaa ttcaacaatt attcgggcaa ctttatcagc gccttctgga ctaaagtggg
    22501 tattgtcttt tagtccttct gggtaatttt tacaagtatc aggtataaga tgcaggtaga
    22561 atttttttgt ctcttctttt gagaaggtct ggaacaattc ttgtgatctt gagaacacat
    22621 ctaaaagagg aatatggtgt ttctcagcaa atgcacgcat cgcttttgga tagtctccaa
    22681 gtatatctgg attcaatgtt ccgttttcta agtaatctcg tcgcgtcaca gatgtcagta
    22741 agaccatttg gacgttcgct ttttttgcgc attcaaaaaa tatctgcaga ttttttaaat
    22801 agctaccgaa gggaagcgta cctctttctt cttggatttt ctggtcgtta tgtccaaatt
    22861 gtacaaataa gaaatcattc ggctgaaact gtgaagctag acgggctaac cttccttcct
    22921 ggataaatga tttggtgctg cgtccattct ttgcttggtt attgatctgg atctcgggtg
    22981 aaaaataagc agagattttt tctccccaac ctgtctcggg acgcttgttt tcatcttttt
    23041 ctgccgcagt agaatcaccg gcaatatgga tagtaggcat gttatctctt ctttcataaa
    23101 ggtttatagg caatgaagca gaacagcaag agaagaagac ggagcaaatt tggtccgtct
    23161 tcttctctaa aacgtggata aacggattcc gttatttcac tatcctgttt ttgattcgtt
    23221 actgttacta ttaatcaatt aggcaaaaaa gccaaagtaa tttttagcat ttgtataact
    23281 aatcgcttga actagacttc ctaaaagttg ttcatcttca ggtgcttctc ctcgcatgac
    23341 catttcgcct aaaaattcac aaagaacacg acggaaatat tcatgtctag tgtaggacag
    23401 gaaacttctt gaatctgtca gcatgccgac aaaattcccc aaagcactac catcagcaaa
    23461 ttgtgtcaat tgatcgcgca taccagctcg agtatcgttg taccaccatc cagaacctaa
    23521 ctgcagtttt ccattctgtt ctttttggaa acagcccatc aaagctaaaa tcgcaggata
    23581 gtcattcgga ttcaatgaat aaaggatcgt ctttggcaac tgatccgttt cttctgcacg
    23641 gttcaataat ttttgcagag gaatagtcaa agaaagatca ttcattccat catagccggt
    23701 atcgggccct aatttttcca acatttgtgt attacagtta cgataagcat ggatgtgcag
    23761 ctgcatcgtc caattatgag aatgatagaa gtgggtcaaa tgtatcagtg tttctgtacg
    23821 ataagcatcg atttcatctg aagataatag tttgttagct cgtgcttttt gaaaaatctc
    23881 ttctaatact gtttcatccg cttctttata gcgtaagatg tctaaagcgt ggtcggaaac
    23941 acgacatcca tgttgatgga agtattctat ccgctgccct aagacttcta tcagttcttg
    24001 ataggtggtg atcgtttggt cagctgcttc ttccagtagt gtgacccatg cattaaatcc
    24061 ttctttttca atattgagtg ctttatctgg acggaatgaa ggtagaacct tcaaatctgg
    24121 ttcttcatga gcaagcaact gatgatagtg caagtcgtct gcgggatcat ctgttgtaca
    24181 aatcacttct acattcgatt tttttgcgat ttcccgacgg cgaaaatcag gagctgccaa
    24241 aatttcattt gcttgtgtcc aaattttctc tgcattttct tcatttatca acaagtcaat
    24301 accgaagaaa cgcttcaatt caagatgtga ccaactgtaa agcggatttc cgataatttt
    24361 tggtacggtt tgacaccaag ctaaaaattt ttcaaaatca gaagcatttc ctgtgatttt
    24421 ttcttcaggc acgccgcaag ctcgcatcaa gcgccatttg taatgatcgc ctcctaacca
    24481 agcttctgtc agattgctga aattgttgtt ttcgtaaatt tcttttggat ctaagtgaca
    24541 gtgataatca ataatgggca tgttttttgc gtaggaatgg taaagctttt tcgcccaatc
    24601 gtcttttaat aaaaaatcat cagataaaaa cataaatctt ccttctttct ttttatactg
    24661 ttttatgaaa atcaaagtgt tactccgttt ttaaaaatgg caatttctcg aacgtcattt
    24721 tgttcatttt tcgtttcttc tccactagct acagcgatga ttttttgaac aaacgattct
    24781 aagattactt ccattggttg ttctaataag acgcctgcat taaaatccat ccaatgacct
    24841 ttgcgttcaa aaagagttgt attcgtagat accttgaccg ttgggacata tgagccaaac
    24901 ggagtccctc ttcctgtggt aaacaagacc aactggcaat cggatgaagc aagagcagag
    24961 gcagctacaa gatcattccc gggagcttgc aataaactaa gccctggttt tctgattttt
    25021 tcgccatatt gtagcacatc aacgacagga gagcttcctg atttttgcgt gcagcctaaa
    25081 gacttatctt ctaatgtagt gatcccacct gctttattac caggagatgg gttttcgtaa
    25141 actggttccc cgtaagacaa gaaatactgt ttgaagtcat tgatcaagtc aacgattgag
    25201 tcaaaaactt cttcattttc cgctcgtgcc atcaatactt gttctgcgcc aaacatttct
    25261 ggcacttctg ttaagatcgt gctgcctcct tgagcaatca agtagtcaga aaatgcacca
    25321 agtaaaggat tagcagttat tcccgataga ccatcagaac cgccgcattt caaaccgaca
    25381 ttcaacttag agagcgggac tgatatgcgc tggtcatttt ccgccgctgt taaaagctct
    25441 tctaatagag ccacaccttg ttcgatctca tcgtaaacat cttgagccac tagaaatttc
    25501 actcgttctt catcatattc acctaaaatt tttttgaatt caggaactgt attgttctca
    25561 caacctaatc caaatactaa aactcctccg gcattgggat gatttactgc gtccgctaaa
    25621 atttctcgag tattttgatg atcttttcca agctgcgaac agccataagg atgctttaaa
    25681 atcgtaatat ggtcaaactg tcccagatct ggatgttttt ccttgaactg tttgacgatc
    25741 agttcagcaa tgccattcac acacccaacg gtaggtacta aaaacaaatc attacgaatc
    25801 cctacttttc cattttttcg caagtaacct tgaaatgttc ggttctcaaa aaggtaatga
    25861 cttggatgga catctttttc atatcgataa tccaattcac cagaaagatt ggtttttaca
    25921 ttatgagtgt gcagccattg accggcagtt acgtctgtcg tcacatgacc gattggatag
    25981 ccatatttga tcacgttgtc tccagctttc aaatcaccca atgcaatctt atgaccttgc
    26041 gggatatctt caagagtggt gatcgtttgc tgatcaatcg tcagttgtgt ttgttttgga
    26101 ataggggtta atgcgacgac cacactgtct cgtcgattta atttcattgt tggttgcagg
    26161 gtttcaagca tggaccatcg ttcctttcat ttcttgtaat actgaccgga cacctttttg
    26221 atcgatcttt gcagctattt cttgaactgt atctgtcaca ttcggtattt gagacaaatc
    26281 ttctccccaa agcgcagcgt tttgcaataa tcgaggaata gctgttccat tatcagacca
    26341 agcatcagta aattcgctta tcgtcttttc atcatcatgt ggctttactt gatcgcctcg
    26401 atacataaga agtagcgcag ctagtgaaac agtgattaac ggtggtacct gattttgttc
    26461 ttcgatatac ttcttgaaca ctggaagcag acgggtgctg aactttgaaa cactatttaa
    26521 tgaaatcgct tctaatttgt gatgggcaaa aggattcaag aaacgttctt tgatttggtc
    26581 agcataaatg gctaattcat ctttaggtaa agaaagcata ggaatcaatt ctagattgaa
    26641 taactgatca acatatgcgg agaataaagg atctttcatt acttcttcta cagtttccaa
    26701 ttccgccaac aaagcaagag ggaccatcgc tgtatgcggt ccattaagca aatgcacctt
    26761 acgttcacga taaggagtca tatcatctgt gatgatgaca ttcaagccag cctgtttgaa
    26821 tggtagttga ttctccaatt ctttaggccc ttcgatcacc caaagcatga atggttctgc
    26881 tttgaccatc aattgatctt catagttatg ccgttgattg aaaacacctg catcttcgcg
    26941 aggataacct gggacgatgc gatcaactaa actacaacag aaaacatttt ctttttctaa
    27001 ccactcaaca aaagcaggtt ctaaatccca tttttctgcg tagcgtaaaa tgatttcttt
    27061 tagacgttcg ccgtttctgt caatcagttc gcaaggaatg atggtaaagc cagctttgtt
    27121 taatttgaaa cgtcggtata aaaaggctgt cagtttgccc gggaagctct gtgggggagt
    27181 atctgttaaa ttatcagtct ccacgaattg gattcctgcc tctgttgtat tggagacgat
    27241 ataggccaag tcgtcatttt ctgctagaga tagataagca tcccattcat cataaggatt
    27301 gatcatttgt tctactacgg tgataatctc ggaagtatca acgatttcgc cattgctgag
    27361 tccttgtaaa atgaccgtat ataaattatc ttgctggttc atcatatttc caagaccttg
    27421 ttctaaaggt tgaatgattg caacattacc tttaaacaat tgttggttat tcagctgctg
    27481 aatctgccaa tcaataaatc cacgcataaa attgccttca ccaaactgaa gaacttttac
    27541 aggtgcttcg ttttttttca ctaattcttt tgtcagtttt ttcatataaa cttcctttct
    27601 gtgttatgca cacgttaaca tttttatata aaattacttg tagtttacaa gtaattgatc
    27661 gactcacgaa ggatcaacaa ggtggagagc tcaattttag attgttcgat cgtcctgcct
    27721 tcgatcaaat ctagtaaaag ctttgttcct tcttgacaca tttctttcaa aggcttacgt
    27781 acggtggtaa gcggcggatt gagataggag acgactggag aatcatcgaa cccgatcaga
    27841 gaaatgttct ggggaacacg gaggccagct aaatgtacag ctctcaatgc accaatcgct
    27901 atgtcgtcgt tgccgcaaaa aaccagagta ggcaggtcag aaagtgataa taggtattcc
    27961 atgttttgga ggccgctttc taagctgtaa tctcctgtta aaaaataggc tggattgaca
    28021 gggatgctcg ctttttttag acttttcaat accccttttt cccgttcttt tgtcgaacga
    28081 aaatttttct ttccgccaat ataagcgatc ttcgtatgtc cgcatccaat accgtaatct
    28141 actgcctgca taacaccggc cgcatcatcg gcagtgacgt tgataaccgc tggatcattg
    28201 atgtgacgat tgacaacaac gaaaggaaat tctttttctt tgagatagtc gatgaaaggc
    28261 tgatcatcat cactttgact cataacgatc agtccatcgt agcgttgcag gttgatttct
    28321 gttaaatcaa caacatcgat actttcaaca gataaactgt aatttgtgtc taatactgaa
    28381 tggatgcctg caatggaatc tgctaggaaa tttgatgaag ttcctttatg caaacttgag
    28441 aaaaatagtc cgattagata ttttttttga ttcactaaac cttttgcact atagtttggc
    28501 gaataattca tttccttagc tagttgtcgt attttttgtt ttgtttcatt ttttattaaa
    28561 ctgctgttat tcaaagcacg agaaactgtc gtatgagaaa cgccagctgc tttagcgata
    28621 tctttgatcg ttaccattcc atcattttcc ttactttttc actttttgtt aacgcattca
    28681 tcatattacg ataactacga taagtcaata gatctttgat atgaaaagat tgcacacgtt
    28741 aaaaattact tggattttta tttttaatta tgctattctt gaaagcgggg aatgctcatt
    28801 taattagatg agtatcaatt ttacagaaaa atgaaagcct tttattttaa aaggaggaga
    28861 ggaagaaaat gaagaaaagt acagtatttt ataaaatatt tttaccgatg tttttattag
    28921 gagccgtact tgtcatagga tttagtttat tcatttacca gaatacctat gaatcgatcg
    28981 aatcgagtta tttaacagac aaaaagaatt tgctcaggca agttaaaact aacgttgaat
    29041 ggaaaattag aacgattgaa tattcgtttt ctacgtatgg ttcgaccaaa aatttctcgg
    29101 acatattcaa gcatccgctg acatatacag attactccac ttattctgaa atcaggaaag
    29161 aactaaattt tatagaaacg attgttatgg atgataataa ttatgattta gtcagtttgg
    29221 ctggtaaatg gggagtaatc aatggatcgt tgagtcaact gaccgaagag gaagttgaaa
    29281 agtatcaaca gaagtacata aataacaaaa ataatttatt ttggcaaaag aaaaacaaag
    29341 gaatcgaaat ggtgatttct ttgccaatgt ttgaaaaaga aaaatatgca ttgggaattg
    29401 ctacgataga gaaacataca atagaacaag ttgttggtga aagtggagaa gacatacttt
    29461 ctattgaaaa cgaagttggc aaccttttta ctactcagac agtaaaaaat aaagagcaac
    29521 caactgtatt aacggacgtg gatctagatt acaatcagct gaaagtgata agagaaaaag
    29581 ataaaacgta tatagtactg aaatcagatt ataataattg gatttacaga ttagaagttg
    29641 actctagtgc aattggcgca atgatccgca atctgagaat cgggctagtt gcagtgtctt
    29701 taacactggt aggactcatt ggtcttcttt cctacttatt ttcggacaga tttgcccgtc
    29761 cgatttctca aatacaggaa cgtctgaatc ttaaatcaaa aggattcagt gggaaagaac
    29821 tagatttggt cgctcaatca gtcagtcgga tcataggaga aaaagaagcg ttaagtgcac
    29881 acttagttac ccaaaagcca cagctggaaa cgctatttgt acttagttta tttagaaatc
    29941 gagtagaaag gcgagaacta gatcagcggt tgcagcaatt tggttatagt tctcaaaatg
    30001 actgctatta tacagctttg gttcaaatcg atctgttaga agatagtcac ggtggggagc
    30061 gtgatttgct acttttagcg atcaacaaca tgatcacgga aattgtgcca aaggaaaatc
    30121 gaatgctgcc gatcgttttg aatgaagaaa tgcaagccac tatttatcga atggataaag
    30181 atgatgcaga agcaagcaaa aaagtcatgg agtattgccg actcattcaa aaacgtgtaa
    30241 aagaatactt gaatttaact atcagtatag gcatcagtaa ccggttcgaa acgctgaatg
    30301 aaagcaagca atcggttgat cgggcaaaag aagccctgta ttatcgtgta aatactggtc
    30361 caagttcaat tattttttat caggaaatcg ttcctgctgc tcatgaaaaa acacttatta
    30421 gatatccgat agaagaacaa aatcgattgt ttgaagccat tcgttctgga aatcaggaag
    30481 tgaagaattt agtacatgaa ttgattgatg cattgtttgc acaaaataaa aatcctttga
    30541 gtcgggaagt agtttcggtc cgcttgatca atgaactcgt tcaattagga cagttattgg
    30601 gagtagattc aaaaaacttt gataacatga aacaaattta tgtaaaagca ctgaacaatt
    30661 atcatcccaa agaactagaa aaacttgttt tagaacaatt agttctgcca atcacagaat
    30721 gctctcaagc aatcactgat catgaattta aaaatttgtc ggagaatatt atgcatatcg
    30781 tctacacaga atatgatcgt gatctgtctt tagatgtgat cgctgatagg ttgcattata
    30841 accctaacta tttaagtaat gtatttaaaa aggaaacagg agaaaaattt ggtgattttg
    30901 ttcaaaatca tcgtttagag gtagcaaaaa aatggttgaa agagactaac ctctccgtaa
    30961 aagagattgc agaaagattg caatatcgaa atccgcaaaa ttttattcgt ttctttaaaa
    31021 agaaggaatc gattacgccg ggagaatatc gaaaaaaata tttgtgaatc aagaggctgt
    31081 ttttcttgtg gactgattca agcaataaaa gaagttagaa taaccctagt atttgtattt
    31141 atggatagtt aaatttcaag gctaagcacg caaaaagaac tgtgacaaaa gtcttgtcac
    31201 agttcttttt tacgaataaa tggcgttcaa gagacaggct atcgacaata aggcataatt
    31261 ttgtgaaata tgggaagcta tttttcaaaa tcgcttctta ttgctcctgt gggttactcg
    31321 tcgcaatgtt tgtgagagtt tttatcatcg ctcaaggctg aacgtctctt tcacaacctc
    31381 tgatacacgg tgttccagga gtagattctt cgaaaaataa gccgaaattt cacaaaaatt
    31441 taagaagcaa ttttcgtaaa tgccttctta tttcttgaag ctgaccactt ctgacacaac
    31501 taccattttg cgttcagtac ataatcaccc tttgacagaa cctacgagtg ctcctttgac
    31561 aaaatatttc tgaacaaaag gataaacaat cagcataggc actgttgcaa taacgataac
    31621 agccatttta actgtttgtg ctggtggaat cacatcaacg gcactacttt ctgcttgcat
    31681 cccacttgat acaatgacga tttggcgtaa aagaatctgt atcggccatt tagatgaatc
    31741 gttgatatac aaaatagcag tcatataagt gttccagtaa gtaacagcat aaaaaagcga
    31801 gatagtagca atagacggta acgctaatgg cagcataatt ttgaaaaata caccaaaatc
    31861 tgtgcaacca tccatttttg ctgattcttc caaactatcc ggcaaggctt ggaaaaagtt
    31921 tctcataatg atcatattca ttgcattaac agccacaggc aggatcatgg cccagtaaga
    31981 attgaccagc cctagagaac gaacaactaa gaacgttgga atcatccccc cgctgaacaa
    32041 catagaaaat acaacaagaa agttgacgaa acctcgacca aacaaatact ttctggaaag
    32101 accgtaggcc atcagtgaag ttgcacacat actgacgatc gttccgacac ctgtaacacc
    32161 aatcgaaaca gcaagtgctc taaaaatggt cggcgtagaa agaatgtagc gatacgcgtc
    32221 taaagaaaag gtagtaggaa aaaggataaa ctttttcgcg acgacttctt gtgtagaagc
    32281 aaaagatgta gcgatgatat ttaaaaaagg aacgatacag atcaaagcta gcaaaattag
    32341 aaacgtattg ttgaaaagtg taaaacttct ttctcctata gagactttct ttttcatata
    32401 gataggctcc tcctcaaaat aaattaaatg tttctgtttc tatatgttca atttatcatc
    32461 ggtgaaagat tacgtatata atcatttcct atgctaagtg tcagatagaa aaaaatagaa
    32521 ggtttgctat gcgtagttta tttgtgaaaa aatacacttt atcgttttgt atagcacaaa
    32581 aatcattgct atgtaagcgt ttttacttac ggtaaaaatg attatatacg taattcagat
    32641 agttatctat catgaagtca taagcagcaa acattcacaa aactaaggag gggcttatgg
    32701 aaagtgtaaa gaaacaagaa ccatttcaac tgacgctatc gaaaaaagaa gaaaagctca
    32761 gaagacgaaa aaaaataatt gcaggaatca aaacgaataa atttttatat gtgatgattc
    32821 tgccgggagt cctttatttc ttgattttta ggtatcttcc tatgggggga ctcgttattg
    32881 cttttcaaga ttatcaacca tttttaggaa taatgggaag tccttgggtc ggtttcaaac
    32941 atttcattcg tttgtttaca gaaccgacat tctttacatt attaagaaat acgttgatct
    33001 tgttcggatt aaatatcttc gtgttttttc cgatgccaat cattttgtct ttactattaa
    33061 atgaagtaac gaacaaacga ttgaaaaacg ggattcaaac gatcatttac atccctcact
    33121 tcatgtcttg ggtaatcatt gtttcgatta cttatgtatt tttgaatgtt gacggagggg
    33181 tattgaatga attccttgca acaatgggat ttgaaaaaat cagttttcta acatcccctg
    33241 aatggacacg cacagtatat atcggacaag tgatctggaa agagttgggc tggtctacga
    33301 tcatctacct ttcagcgata actgcagtcg atacacagtt gtatgaagct gcagaaatgg
    33361 atggagcagg acgcttacgc aaaacatggc atgttacatt gccggcaatt cgtccagtaa
    33421 tcattacctt gctgattcta aaaatcggtc agacattaga cttagggttc gagcatatgt
    33481 acttgttatt gaattcattg aatcgaagcg ttgcagaaat tttcgacacc tatatttata
    33541 cagctggatt gaagaatgga cagttaagtt tcagtacagc aatgggattg tttaaagggg
    33601 tcgtaggatt gtttatggta attttagcta ataagattgc caaaaagcta ggagaagacg
    33661 gcgtttacta agatagaaag agagggaaag atatgctgca gtcattagat aaaataaacg
    33721 gaaggctaaa tcgtatttta cagttggttt atttgaattt tctgtggggc ggtttagtca
    33781 tcgcaggttt gggagttttt actttcggtc ctgcatcgta tgcgatggtt gcagtgatta
    33841 gaaaatggct gcgctcaaaa gaaacgtttc ctttgacgaa aacgtacttc acatatttta
    33901 aagaaaacta tcgtgaaacc ttgctgatga gctggatata tggcgccatc gggattgttt
    33961 tacttgtgga tctttctttc atacacaatt ggtatcttag gatcgctttg tatattgctg
    34021 cgttcatata tgtgctctca gcactctata ttttcccaat catggctcat tatgattgga
    34081 aaggatattt tttcaaaatg aaaatggcat ttttattcgg attctcacat cttcattact
    34141 cattagtgtt gatattaagt atggtaggga tttatctgat gatcatccaa acaatgccag
    34201 gtgttttaac ctttatcggt ggcagtctct tcttttccgc agtcacgtgg acagcaaatt
    34261 ctatattttg ccatttggaa tggaagaact catatacaga aaaaacagaa gaaaacaaaa
    34321 atattattaa aacagtgaag gagaaaattt atgaaaaaag caacgctcgt aaaattaggt
    34381 cttagttttg caggaattct agcacttgcc gcatgcggca gcagttcaag taacagttct
    34441 tcagagacag aagatggaaa agctaaaatc acttggttaa atattttgca tcatgcttca
    34501 ccgcctacag acacgattgt gaacttgatc gaagaaaaaa ccgatacgga actgacattc
    34561 aactggattc ctgatgcttc aaaagaagaa agattaacaa ctgcattggc ttctgatgaa
    34621 ctaggagata tcgttacact tcaaattgcg atgttgaaaa actcttctgt ccgcaatgcg
    34681 ttaaaatcag gcgtattctg ggatgtatca gaatatttaa aagactataa aaatttaagt
    34741 caaatttctg aagatagaat cgaagctgcg agtatcgacg gtgccttgta cggcgtaccg
    34801 atacaaaaag actacgctcg tgctggctta gtcattcgaa aagactggtt ggataatcta
    34861 ggactagatg ttccaactac catggatgag ctttatgaag tagctaaaca atttacagaa
    34921 aacgatcctg atggaaatgg tgtggacgac acagttggtt ttggcgatcg tgcgcctagt
    34981 gatattcgtt ataccagctt taaacttttc acttcttatt ttggagcacc aaatggctgg
    35041 gctgtagacg ataatggcaa attcactcca gaatttgata cagaagagta tatgaaagct
    35101 ttgaattttt caagagatct atttgagaat ggctatttgg cacaagactt tgcagttacc
    35161 caaaagactg atcagcaaga acaatttgca caaggaagaa ctggaatcta cactggaatg
    35221 gtggatatca agaatctgaa gacgatgggt caaggtattc aagatgatat ggaattagtg
    35281 cctgtaaaca agatctcaga tggtgaaggc ggcggttatc atgtttggtc agaaggaaat
    35341 ggtattggcg gcttgctagc atttcccaaa tctcaagtaa aagacgaagc acaattgaag
    35401 aaattacttc aatttgttga tgatctttta gaagaagatg tgtataaatt gatgactggc
    35461 ggtatcgaag gaacgcatta tgaattgaat gatgatggtt ctatcgaatt tatcgatatg
    35521 gatctttggc aacaagaggt gcaaccgtta tcaagctctc gtcctagtga agtgacgttc
    35581 tctttcaaag atacagaccc tgaaaaagaa ttctctaatc aattgatcaa agaaaatgaa
    35641 gaatttgctg taatggatcc ttctgtgcca ttggattcac cgacgaacaa tgaagtagga
    35701 tcagaaattg aaaaaatcgt cattgatgct acggtgcaat acatcatggg acagcttgac
    35761 gaagatggat tcaaaaaagc tgtagaagac tggaaagcac aaggaggcga tcgaatcaca
    35821 caagagtacg aggaagcata caaagcagcg gcacagtaat atagacgatt gggacaaata
    35881 cttcttggta tttgtcccaa atcctatttt aatttttaaa ggagagcaaa taatggaaaa
    35941 ctggtcaaag aaattagcaa aaagtatcat ggaaagaaca cctcgcttat atgaagaaaa
    36001 gtggtataaa ggaaaatggt cttatgacta tggcgtcgta cttaaagggt tccaattact
    36061 ttgggaacga acccaagaaa aaatttattt tgatttcata aaagacaata tcgactattt
    36121 cgtgcaagaa gatggaacga tccgtggcta cagcgttgaa gaatacaata tcgaccatgt
    36181 aaatacagga aagcttttct ttttattgta caaagaaaca ggcgaagaaa aatataaaaa
    36241 agcagcagaa ctcctttccc agcagttggc aaaccatcca cgcacatccg aaggagcttt
    36301 ttggcataaa gaaatttatc cttatcaaat ttggttggat ggcttatata tgggttctcc
    36361 tttttatgct gaatttatcc agaattttag tgaagaaaaa gattaccaag atgttttgag
    36421 acagtttgaa atcagttata aacatctatt cgatccaaaa acaaatttgc tgattcatgc
    36481 atgggatgaa aagaaggtcc aaccatgggc tgatccaaaa acaggtctat cccatcattt
    36541 ttggagccga tcgattggct ggtatttgat ggctgcagca gatacctatg aattacttca
    36601 tgaagaaaca gattgttctt tgctgaaaga aattctagaa aagacactgg ctgcattact
    36661 tccatatcaa gaagaaaaca gcgggacatg gtatcaagta acaacagaaa caaaacggaa
    36721 aggaaattat ctggaagctt ccggcagtag tatgtttgtt tacgcaatgg ctaaaggaat
    36781 acgtttggga ctgctgaaca aagaagaatg gcttccacaa gtgaagaaat cacatcaagg
    36841 attacttgat gaatttgttc tggaaacaaa agaaggctgg ctgaacttga ataaaaactg
    36901 tcaagtagca gggttaggcg gagcagatca gcgagatggt tcatatgctt actacatcag
    36961 tgaaccaatt atttgtaatg atcaaaaagg cgttggtgca ttcttgcaag cattgattga
    37021 agtggaggca ttataaatat gtatgatatt ttgacatttg gcgcatcaat tgatgaattg
    37081 aatactgaag caatacaaca agccattgat gctgcagcaa gtgacggagg cgggaccgta
    37141 gttgttccag caggtgaatt tctaacagga gctttatttt taaaaagcaa tgtcgaactt
    37201 catttaagcg caggagcggt tctgaaattc tctgacgatc caaaagatta tcctgttgtt
    37261 cattcacgtt gggaaggagt ccatcgaaaa gtttatgctt cctgtatcta tgctcaaaat
    37321 gtagagaaca tctctgtcac tggtttcggt acattagatg gaaatggaaa aaaatggtgg
    37381 catactttta gaaatgaacc agataatctc gcttatcctc gtcctaaatt gatgagtttc
    37441 cataactgtc atcgaattac tgtgaaagat attaaattga tccagtcacc aagttggacg
    37501 atcaacccga ttctttgcag taatgcaacg tttgataact tgacgatctt gaacccagcg
    37561 gattcaccaa atacagacgg aattgatcca gaatcatgca aaaatgtgcg tattagtaat
    37621 tgccatattg acgtgggaga tgactgtatt gcaatcaagg cagggactga agatacgtac
    37681 gaaaggatcg cttgcgaaaa tatcacgatc acaaattgca cgatggtcca tggacatggc
    37741 ggtgtcgtat taggaagtga aatgagtggt agtattcgta atattaccat ttcaaattgt
    37801 atcttccaag aaactgatcg aggaatacga ttgaaatcta gacgaggtcg aggcgggatc
    37861 gttgaagata ttcgagtatc caatatagtc atggataatg tgatgtgtcc atttatactg
    37921 aatctttatt atttctgtgg tccgcgaggg aaagaaccgt atgtatggga aaaaaaggct
    37981 tatccgatag atgaacggac accagcattc agaaggatcc atttttcgaa tatcacggca
    38041 aggaatgtcc atgcttctgc aggttttatt tatggattag cggaacaatt catacaagaa
    38101 atcacatttg acaatatcga tgtctcgatg gcccaagatg cagttcccgg tgttcctgct
    38161 atgatgacag gaatagagga aatgagcaga caaggatttt atattggctt tgcgggtaag
    38221 gtttccttta accagatgtc gattgaaaat cacgagggac cagcatttct attagaaaat
    38281 actaaagaaa tcagtattga gaaattccaa tcaaatcaat cagaaaatat ggatgaactg
    38341 gttgttcgta aaaactttgc ttatgcagtt gaatgatttt ttaggtgctc catctagcag
    38401 acatacccct agtggaaaac gattataccg agaaaaacaa aacggtttta tgcttgaata
    38461 ctatcaattg tacttaaatg aattggaaac agtaccagcc ctctatgctt atccagaaaa
    38521 agaaggaccg tttccgccgg tcctttacct gcattctcac ggcggagatt tttcagtcgg
    38581 gaaaagtgaa ttgatccatg gtgctcctta cttagcttct ccatcttttg cagaagtact
    38641 gacaggaatg ggatatgctg tttggtcgat tgatgcttgg ggatttgaag aacgcggagg
    38701 tatttctgaa agcgagttgt tcaaacaatt tttattaaca ggaaaaacgt tatggggaat
    38761 gcgtatttat gatgtgatca gcttgatcga ttatttggag acaagggaag acacagatac
    38821 tcaacggctc gcaacgattg gtctttctat gggaggctta ttaagctggt gggtgacagc
    38881 attggattct cgtgtcaaag tttgtattga tctagcggca caggtggaca tcgaaatact
    38941 tttgctacat cgccgcctag atcatcatgg gttttattac tatgtgccaa atctattgac
    39001 agcttataca acgttagcaa tccaagaaaa aatcgctccg cgccctcgtc ttagcttagt
    39061 tggaaaaaac gatacgatgt gcttggacga aggcgtactg aagctgagaa aagggctgga
    39121 caaaaagtat caatcaatgg gtagtccaga aaacttttct tctttttctt taacaggcgg
    39181 tcatatggaa acacaagaaa tgagaaatat ttggaaacaa ttcataaggg agcatttatg
    39241 aggatgaaag acaaactgat aattggaaag aatgctatag ataccccgac ggattttgaa
    39301 actatccaag aaggaatcga ttatttggca ggcctgccgg atagccatcc aaaggtcttg
    39361 atagtaaaag aaggaagata tgatgaatat atagaaagca gattatccaa ttttaaactt
    39421 gtgggagcag gaacagtagt tatctccggt aatcgttttg caaaacagga gtatcttgac
    39481 ggtagccagt tagggacttt ccgaagtgct acttttttct tagaaggatc gaatgtcgta
    39541 ttagaaaatc ttcacatcga aaatacagca ggtccaggag aaaaggtagg acaggctgtt
    39601 gctttattca attcaggaga tcgcgtatct ttaaaaaact gtcgactatc tggttatcag
    39661 gataccttat gcacgggtcc gttgcctgat ttgcaaaaag atggtactcc tttttgtaca
    39721 ccggcttctg ccaatcctga gtattgtcgt cagacctatg aatattgctg gattgaagga
    39781 acggtggatt tcatatttgg tggggcagat gccgtttttc atcagtgtca tatccatagc
    39841 ttagccagta aaaatcctgg atacatcaca gcagcatcta cgccgaaaaa tcaaaaaaca
    39901 gggtatcgtt ttgaaagctg taccttgact gcagaacctg atactagaaa cgtctattta
    39961 ggaagaccgt ggcgtccgta tgcaaatgtc ttatttgaag cgtgtaagat aggtgggcat
    40021 gttcacccaa ctggttggga taattgggat gacaagaaaa atgaacagac tgtcaaattc
    40081 tctgaacgta ataacaatta tgaaacatcg atcaaaagac ctgaatggat acatttagaa
    40141 aagcaggggg ttcagaaatg aaaagaaaat gggaactatt gctaggaatg gtcggtggca
    40201 gtctatcttt gattttcttt ggcgggttag cagttacttt aagtaatatg tctgcgtcag
    40261 agtttaaaaa aagttatcag tcgcttgctg tagatcattc aactttgtct ttagaaaata
    40321 catttggatt attacaggac atgactggtc tgtttgccgt tgtgttgttt atttcacttg
    40381 cttttttagc agtagcattg tttttgactg ctaaaggaaa atacttaaca actgccactg
    40441 gtttgtattt tataacagga ttcatattgt tgattggcac tcagtttatt gcgtttcctt
    40501 ttgcattttt ttatttcgca gctggggcat tcagtttata tcgagtaaga atgagaaaag
    40561 gagcctgaat catgcagact atcaaagtga aaaatcctat tttaagagga ttcaatccag
    40621 atcctaatat attaagagtg aaagagcggt attacattat tgtctcctct tttgaatggc
    40681 ttccaggaat ccgagtatat gagtcgaaaa atttagtcaa ctggtcacat acaacggata
    40741 ttttgaccac tcaagtatct ttgcaaggaa atcctacaaa tggaagtata tgggcgccac
    40801 aaatcagtta tgctgatgga ttgttttatc ttgtttacac agatgtcaaa agtgcaggcc
    40861 gtccgtttaa agatgtccac aattatttga tcactgcatc tgagatcaca ggtccttggt
    40921 ccgaaccaat ttatttaaat agtaacggct ttgatccttc tttgttccat gatcttgaaa
    40981 cgggaaaaaa atggcttgtc aatgaactat gggattatcg tctggacaca ccgaataaat
    41041 ccagcggtat cgtattacaa gaatatgatg cagagaagaa gaagcttgat ggtcccatct
    41101 ataaaatatt tgcaggtaca gaattagcga aaacggaggc accacatttg tattttcgta
    41161 acgggtatta ttatttgatg acggcagaag gcggtacagg aagaggacat tcagttacag
    41221 tttgccgagc aaagaatatt actggtcctt acgagcttga tcctggtttt cctatgctga
    41281 cagctagtga caaacctgct tctacattgc aatgtacagg acatgggagc ttagtacaaa
    41341 ccccgtcagg aagatggtac atgacatatc tttgtaccag accattaaac ggtgcggcta
    41401 ttttgggaag agaaaccgct attcaagaag tgtattggac gtccgacgat tggttaagac
    41461 ttaaagatag ccagacaact ccgatggaag aaataatgat tgagacaaat gaagaggtac
    41521 aacaagaaaa agatcatcag tttatggata catttgaagg agagctaaag aagaactgga
    41581 atgccagacg cattttgcca aatgctgatt ggtgtgatac aaaatgtaga ccaggttttt
    41641 tacgtctgat ttccggtgaa tctcttcaat ctaccttcga acatcatttg ttagctattc
    41701 ggcaaacgga ttttactttt gatgcggaga cagcatttga atacatgcct aataatttta
    41761 atcagatggc tggactggta ctgtacttaa atgaatccaa ttatatttat tgttatgtta
    41821 cttggaatga ggacaaggga aaatgcctac gtatgataga atcagaaaac ggagtagcac
    41881 aaatagaaga ttggacgatt ccactcacag aaaagaagac ttttttgaaa gtcactgtaa
    41941 agaaagagac agcacagttt catttgaaag acgtatcaac tgaagaatgg aagaaagtag
    42001 gtccgcaaaa aaacatgtgt atactctcag gagggtttac tggaaatttt atcgggatag
    42061 cagtgcatga tctaaataaa aaaagaggaa gttatgctga ttttgagttt ttcaagtatg
    42121 atggaaaaga tcatctgtaa tttaattctt taataaatga ggttcactaa cttattttca
    42181 ctagttagtg aatttttttg tttgtatata ttgttatgta aaaaaagtta tcaatcttat
    42241 gcatcaatgt ttttagatta ttttttgttt tttatctgtc aagataatgc aataaaaagc
    42301 caacattgtg aatataaaaa caagaaaaaa agagttataa ttttgaaaac gtttactaga
    42361 agaggtagga ggaattcaaa tgctttcaca aaatgctgtt acagcaaata caataaaaaa
    42421 agaaaaaaag gaatttggga tttccgataa gataggctac atgttcggtg atctaggtaa
    42481 ctgttttatt ttaggtttag tcaatagttt cttgatgatt tattatacga atgttcttgg
    42541 cgtttcaggg gcaatcgtag gtacgttatt cttgatttcg agaattttgg atgcgtttgc
    42601 tgatgtgatg gttggaagat tatgtgatgt ctcagagtta acagaaactg gccgatttat
    42661 tccttggatc agaaaaatga agtatccatt ttgcttagtg actgtcattt tgtttttgcc
    42721 attcgtccaa catcttccaa tgacggtgaa gattgcttat atttttgttt catacattgt
    42781 ttatgggatt tttctctcaa caatcaatat cccgtatgga gcaatggcag cagcaattag
    42841 ctcaaatcct aatgaccgca cctcgttgtc cacgttccga agcattggtt ctgcgattgg
    42901 cggatcaact acaggattct tcattccgat tttaatgtat acccataaca agagtggaca
    42961 acaagtcgtt tcagggaatc atttcttttg gatttcaatc ggatgtgctg caatagcatt
    43021 tgtcgcatat atgttaactt gtaaattgac tactgaaaga gtaagagttg tcaaaaacga
    43081 aaaagtatct gcttctcagt tggtaaaagg tttgttagct aatcgagcac tgattatttt
    43141 agtcattgtt gacattttca ttgtgatcaa tcagatttta gctgggacga atatgactta
    43201 tttattcaat gattacttcc ataataaaca agcaatgtct gttgctcttt tatttactta
    43261 cggaacagta gtagttttag caccatttgc tacaaagctg acaaaattat ttggtaaaaa
    43321 ggaagcaagc gtatttgcgt tacttgcttc ttcatgtatg tacttgctga tgtatttcct
    43381 tcatataaca aatgcatggg tatatctgat tcttttattt atcgctactt taggatctgg
    43441 tttattcaat ttgatggtct gggcattcat tactgacgtg attgattatc atcaatataa
    43501 gacaggctta agagaagatg gaacagtata tggagtaaat tcttttgcga gaaaagtagg
    43561 acaagcatgt gcaggagcgg taggcggatt tatgctggca ctcattggtt accaatcgtc
    43621 tacaacaggt ggagccatcc aatcagtttt agtacaagaa aggatttata cgatggcaaa
    43681 cttgttacca gcattctgct tatttgtatc cgcaatcatt ttgctagtag gttacccact
    43741 gaatcgttca aaaactgtat ttattggtga agaattgaaa aaaatcaatg aaagataggg
    43801 agaaacgtat tgcggacaaa aaaagggatt atcaatgctt tgaactttaa aacaaacgac
    43861 actcaacaag atgatacaat aatactgcaa aaagcaatcg atcaaggtgc gaaagaacac
    43921 ttacctgtat ttattccaaa aggcatttat ttagtcggag cccttttttt aaaagataag
    43981 agtcatctta tctttgaaga aggtgcagtc ctcaagggga gaacagatat cgaagcattt
    44041 ccagaaatcg atacacgagt agccggcgta gaaatgaaat ggccagcagc gattttgaat
    44101 gttctctcgg cgaaagatat tcttatagaa ggaaaaggaa taatcgacgg tcaaggagac
    44161 cattggtggg agctgtattg gggaaaagat caaaaaagcg ggacaagagc tgaatacgat
    44221 caaaagggac ttcgttggat tgcggattat gctattaaac gtccacgcgc ttgtttactg
    44281 tatcatgcag aacatgttgt tgttcgtgat ttgatttttc agaaatcagg tttttggaac
    44341 ttgcaaatca cttattctaa cgatgtatta gtagaaaaag taatcatccg caacaatgat
    44401 ggaccaagta cagatgggat tgatattgat tcttcaacaa atgtgcgtgt ctatgaatgt
    44461 gatttggcct gtggtgatga ttgtattgct attaagtctg gcagagatgg caatggtgca
    44521 agagtcaatc gaaaatcatc aagaatcgag gttgctagat gtaaaatccg ttcaggatac
    44581 ggtgtaacta ttggtagcga agttagtgct ggagttagtg acgtatatat tcacgatatc
    44641 gatttttttc agtcagactg tggtattcga atgaaatctt caaaagaaag agggggagtg
    44701 atcgaaaata tcagggtaga aaatctgaat atgattgatg tacaatttcc tttttcttgg
    44761 ataatggact ggcataatga atacaataga aaaaatagca atgaccttgc tcaaatgcca
    44821 gaaagttggc aagcagtggc tgaagaaata cctgaatcaa agcaaatgac gaaagtcaaa
    44881 gacatacaca tcaaaaacgt acaggcatgc ctttctccag gttactcact tccttcccga
    44941 gcttttgatt tagtagcatt tcctgaaaag cctatagaag atgtctgctt ttctcattgc
    45001 caaataactg caaaagaatt cgggcgaatc gaagcagtac aaaacttatg tttcaaaagc
    45061 tgtgatttat ctatagaaac tggcaacaca gctgcaaaca atacttttga caatcgttga
    45121 aattataata aggtcaagct taaataatat tttagcttga cctttatttt ttttaatgat
    45181 atattaaaca acgttgtcgc gcttcattga aacattttat gaaaattcca aaaaagagtt
    45241 gacacatgtt tttagacgtg ataagataac taagtcgtta aaaagaacca cttgcgaagc
    45301 ttgattctat cgaaaaaaac ttcaaaaaag ttcttgacaa ataacaaacg ataagttatg
    45361 ataaatgagt tgctgaaaga tttcgaaaaa cgaaatcgaa aaaaactttt aaaaaagagt
    45421 tgacaatcgc ttgtctctct gatatgatat aaaagttgct acggcagcca aaaacgaatc
    45481 gggaaacact cc
//
LOCUS       DASJTC010000009        42754 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.009, whole genome shotgun sequence.
ACCESSION   DASJTC010000009 DASJTC010000000
VERSION     DASJTC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.009"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(122..499)
                     /locus_tag="U4B19_000484"
     CDS             complement(122..499)
                     /locus_tag="U4B19_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076251.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            755..1483
                     /locus_tag="U4B19_000485"
     CDS             755..1483
                     /locus_tag="U4B19_000485"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEN2076252.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1483..1737
                     /gene="purS"
                     /locus_tag="U4B19_000486"
     CDS             1483..1737
                     /gene="purS"
                     /locus_tag="U4B19_000486"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEN2076253.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1739..2410
                     /gene="purQ"
                     /locus_tag="U4B19_000487"
     CDS             1739..2410
                     /gene="purQ"
                     /locus_tag="U4B19_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEN2076254.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2411..4633
                     /gene="purL"
                     /locus_tag="U4B19_000488"
     CDS             2411..4633
                     /gene="purL"
                     /locus_tag="U4B19_000488"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEN2076255.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4618..6057
                     /locus_tag="U4B19_000489"
     CDS             4618..6057
                     /locus_tag="U4B19_000489"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEN2076256.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6080..7132
                     /locus_tag="U4B19_000490"
     CDS             6080..7132
                     /locus_tag="U4B19_000490"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEN2076257.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7129..7707
                     /gene="purN"
                     /locus_tag="U4B19_000491"
     CDS             7129..7707
                     /gene="purN"
                     /locus_tag="U4B19_000491"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEN2076258.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7704..9239
                     /gene="purH"
                     /locus_tag="U4B19_000492"
     CDS             7704..9239
                     /gene="purH"
                     /locus_tag="U4B19_000492"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEN2076259.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9255..10529
                     /gene="purD"
                     /locus_tag="U4B19_000493"
     CDS             9255..10529
                     /gene="purD"
                     /locus_tag="U4B19_000493"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEN2076260.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10651..11565
                     /locus_tag="U4B19_000494"
     CDS             10651..11565
                     /locus_tag="U4B19_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2076261.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11698..13263)
                     /locus_tag="U4B19_000495"
     CDS             complement(11698..13263)
                     /locus_tag="U4B19_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2076262.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13256..14707)
                     /locus_tag="U4B19_000496"
     CDS             complement(13256..14707)
                     /locus_tag="U4B19_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2076263.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14721..15737)
                     /locus_tag="U4B19_000497"
     CDS             complement(14721..15737)
                     /locus_tag="U4B19_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2076264.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15957..17045
                     /locus_tag="U4B19_000498"
     CDS             15957..17045
                     /locus_tag="U4B19_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEN2076265.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17099..18625
                     /locus_tag="U4B19_000499"
     CDS             17099..18625
                     /locus_tag="U4B19_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEN2076266.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18627..19832
                     /locus_tag="U4B19_000500"
     CDS             18627..19832
                     /locus_tag="U4B19_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2076267.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20089..21066
                     /gene="guaC"
                     /locus_tag="U4B19_000501"
     CDS             20089..21066
                     /gene="guaC"
                     /locus_tag="U4B19_000501"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEN2076268.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21330..22754
                     /locus_tag="U4B19_000502"
     CDS             21330..22754
                     /locus_tag="U4B19_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEN2076269.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23215..24324
                     /gene="pdhA"
                     /locus_tag="U4B19_000503"
     CDS             23215..24324
                     /gene="pdhA"
                     /locus_tag="U4B19_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEN2076270.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24327..25304
                     /locus_tag="U4B19_000504"
     CDS             24327..25304
                     /locus_tag="U4B19_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEN2076271.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25329..26972
                     /locus_tag="U4B19_000505"
     CDS             25329..26972
                     /locus_tag="U4B19_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEN2076272.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26980..28386
                     /gene="lpdA"
                     /locus_tag="U4B19_000506"
     CDS             26980..28386
                     /gene="lpdA"
                     /locus_tag="U4B19_000506"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEN2076273.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28548..28823
                     /locus_tag="U4B19_000507"
     CDS             28548..28823
                     /locus_tag="U4B19_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEN2076274.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28829..29596
                     /locus_tag="U4B19_000508"
     CDS             28829..29596
                     /locus_tag="U4B19_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEN2076275.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29764..31593
                     /gene="typA"
                     /locus_tag="U4B19_000509"
     CDS             29764..31593
                     /gene="typA"
                     /locus_tag="U4B19_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEN2076276.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31854..33626
                     /gene="recQ"
                     /locus_tag="U4B19_000510"
     CDS             31854..33626
                     /gene="recQ"
                     /locus_tag="U4B19_000510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEN2076277.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33740..34042
                     /locus_tag="U4B19_000511"
     CDS             33740..34042
                     /locus_tag="U4B19_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEN2076278.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34045..35208
                     /locus_tag="U4B19_000512"
     CDS             34045..35208
                     /locus_tag="U4B19_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN2076279.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35234..38662
                     /locus_tag="U4B19_000513"
     CDS             35234..38662
                     /locus_tag="U4B19_000513"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEN2076280.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38683..39807
                     /locus_tag="U4B19_000514"
     CDS             38683..39807
                     /locus_tag="U4B19_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076281.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39797..40234
                     /locus_tag="U4B19_000515"
     CDS             39797..40234
                     /locus_tag="U4B19_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2076282.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40218..40556
                     /locus_tag="U4B19_000516"
     CDS             40218..40556
                     /locus_tag="U4B19_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEN2076283.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40675..41235
                     /gene="rsmD"
                     /locus_tag="U4B19_000517"
     CDS             40675..41235
                     /gene="rsmD"
                     /locus_tag="U4B19_000517"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEN2076284.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41232..41723
                     /gene="coaD"
                     /locus_tag="U4B19_000518"
     CDS             41232..41723
                     /gene="coaD"
                     /locus_tag="U4B19_000518"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEN2076285.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41713..>42754
                     /locus_tag="U4B19_000519"
     CDS             41713..>42754
                     /locus_tag="U4B19_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEN2076286.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT        14099 a         7332 c         9549 g        11774 t
ORIGIN      
        1 gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc aataatagga
       61 acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa aaatagagga
      121 attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga tgttgcagca
      181 agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg atacgataat
      241 gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct ttgtgttctg
      301 tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct cggcggattt
      361 ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg atcgacagca
      421 aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa agctgacctt
      481 gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa atttttagtt
      541 aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta taagattttc
      601 gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca tatttttgtt
      661 gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa aacgatgtat
      721 ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac ttttgtatga
      781 aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga tagaatattt
      841 agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa aaggagaatt
      901 gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa taaaaaatca
      961 ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa ttatccctct
     1021 ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag gtgttcccga
     1081 aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc agttggatga
     1141 tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg aagaaatcag
     1201 tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat ttgctaaggt
     1261 cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac aaattttgct
     1321 tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga atgaacatat
     1381 ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc aagaagtata
     1441 taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt cgtaaaagta
     1501 tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa aaaagcagtc
     1561 catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt tgagatacaa
     1621 cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga caagctgctt
     1681 gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga agtataacat
     1741 gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt tatgggcagt
     1801 tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct ctttagaagg
     1861 atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga gatgcggtgc
     1921 gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag aagggaaacc
     1981 agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt tgccaggtgt
     2041 actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga aagtcaaccc
     2101 caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc cagttgctca
     2161 tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa aaaataatca
     2221 gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg cagggattac
     2281 gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag tagaagactt
     2341 gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact tcggaaaggt
     2401 gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga aaaaaagaat
     2461 ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag atatcttagg
     2521 aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg aacattgttc
     2581 ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc atgtgttgca
     2641 aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag ttgtcttcaa
     2701 agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg cgacaggagt
     2761 tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt tgctagattc
     2821 attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag aagtagttgc
     2881 aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg aaattgcatt
     2941 tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt tgattgaaca
     3001 caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt atgtaggtgc
     3061 aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat ttaatcaaga
     3121 agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa agctgctgct
     3181 tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta tacaggatat
     3241 gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg gctcaggact
     3301 tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt atgaaatgat
     3361 gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg aagaaaaaat
     3421 ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag taacagacga
     3481 cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag tcgatgcatt
     3541 agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa tcgtagaatt
     3601 tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat tgcttgatct
     3661 gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg attcacaagt
     3721 acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg ttagagggac
     3781 gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt tggatccaaa
     3841 aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta gcggtggtaa
     3901 gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag aagttttttg
     3961 ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag gaactcctgt
     4021 tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct atccaacacc
     4081 aatgattggt atggtcggat tgattgagga tcataaacat atcactactc aggaattcaa
     4141 aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg atggcagtga
     4201 attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg atctatccat
     4261 tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga ttgaaagtgc
     4321 tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt tcaaacatca
     4381 gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag aaactcaatc
     4441 aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa tgatgggaga
     4501 tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt cagcaactga
     4561 cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg caatcgtatg
     4621 tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg gggtcatcca
     4681 gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg acaagaaggt
     4741 gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt ggggctgctt
     4801 tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc tgcaatcgga
     4861 catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc ttttctattc
     4921 aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa tgctcgttcc
     4981 cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga tacggagata
     5041 ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt aaaaagtgct
     5101 ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc aatgattgcc
     5161 gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa cggagcatac
     5221 gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat aagagatgtc
     5281 cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg ctttacgaag
     5341 gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc agattcaaac
     5401 atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt agcgaaagaa
     5461 tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc agccgcaagc
     5521 ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa tcaatacatt
     5581 gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg aatgaaatta
     5641 tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga ttcaattgtt
     5701 cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc aaaagaagtc
     5761 catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat tgatattcaa
     5821 acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga atgtatcggt
     5881 gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg tctggaaaaa
     5941 gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc gacgccgtta
     6001 tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt cttttaaaag
     6061 cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag ctggcgtaga
     6121 tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa aaacggaacg
     6181 tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat cggtctttca
     6241 attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat tagcagtggc
     6301 aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt gtgtcaatga
     6361 tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa cggggaaaaa
     6421 tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg ttcaaggaaa
     6481 tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag gtaatgatta
     6541 tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga caggagaaac
     6601 gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt caaatgggta
     6661 ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat caaaactgcc
     6721 agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct atgtttcttc
     6781 cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca ctggtggagg
     6841 gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa tcaaacttgg
     6901 ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa ttcctgagat
     6961 ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc aagaatcgaa
     7021 agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg aaatcgggaa
     7081 agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat gagaatcgct
     7141 gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct atcgaaaaaa
     7201 ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata tgttttgaaa
     7261 cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt tgattcaaaa
     7321 aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga tttgatcgta
     7381 ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga taaaagaatc
     7441 atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat acgtgatgcc
     7501 tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca aggtgtagat
     7561 actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac gttcgattct
     7621 ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat tagtgaaatt
     7681 attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca gataaaactg
     7741 ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt tcaacaggag
     7801 gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa gaagtgacag
     7861 ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc catggtggtc
     7921 ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa aatatccaac
     7981 caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg aaaccagatg
     8041 tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg ctccgtagtg
     8101 cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat gaagaagtac
     8161 tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa ctagcggcaa
     8221 aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta acggattggg
     8281 tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa acgcttcgtt
     8341 acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg gaaaaatatt
     8401 cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat atccgagatg
     8461 cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc gccttgaaac
     8521 acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg gaagcagcgt
     8581 atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga gaagtagatc
     8641 tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc ccttcttata
     8701 cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg acagtagact
     8761 ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt ggacttcttg
     8821 tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg acaaaacgta
     8881 agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc aaacatgtga
     8941 aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga gccgggcaga
     9001 tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa atggaaggtg
     9061 cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat gcagccaaac
     9121 atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat tctattgata
     9181 tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt agacattaga
     9241 gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa catgcaataa
     9301 gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca ggaaatccgg
     9361 ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat tcagcgttaa
     9421 tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa gttcctttgt
     9481 taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt ccgacaaaag
     9541 cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat acttatcaaa
     9601 tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg tatatcaaag
     9661 aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa ggtgtagtcg
     9721 tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac cagcatcgat
     9781 ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa gaattctccc
     9841 ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa gatcataagc
     9901 gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat tcaccagtac
     9961 cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa ccagcggctt
    10021 tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt ttgattgcta
    10081 cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct gaaacgcaag
    10141 tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta ttagagaatc
    10201 gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg gttgcagctg
    10261 ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg gaagaagagc
    10321 tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga gcaggaggac
    10381 gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa aaaatttaca
    10441 ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt ggagaaaaag
    10501 ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa aaaatacaac
    10561 aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt ggtacacttt
    10621 tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg tacttctgat
    10681 cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga agatccagta
    10741 aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc agatatttat
    10801 ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc aatcaaacga
    10861 gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt tgatttttct
    10921 aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat ggattatgtg
    10981 gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt agctgaagcg
    11041 tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa tcaaaaacca
    11101 atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa tcagctgcaa
    11161 tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat gacagatgaa
    11221 gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga tatgacgatc
    11281 caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg gaacgaaaaa
    11341 ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac gacaccaact
    11401 ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat cgccggtacg
    11461 atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat gtctcgggaa
    11521 gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt gtcataagca
    11581 ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt cagcttcgag
    11641 atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt tcgtttatta
    11701 gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa tcccttgttt
    11761 tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa aagcaatatc
    11821 tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc tttgttctgt
    11881 cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga aatgagaggg
    11941 acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg taaaattttc
    12001 tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg aaggctgtga
    12061 gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa gcgagtcgtc
    12121 ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt gcgggatttt
    12181 cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac gatataatcg
    12241 tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag atacttcgtt
    12301 tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat caactggaag
    12361 tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg aactggcggt
    12421 ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc tttctgcgac
    12481 aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat ggaccaacgt
    12541 ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa ctattaaaaa
    12601 tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc taaataaaaa
    12661 cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt ggtttgctgc
    12721 taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag caagtttcaa
    12781 tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat tttttttcaa
    12841 ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta aagcagttac
    12901 tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca gataatcatc
    12961 tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac taataaatat
    13021 gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc cgtccatgaa
    13081 aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg aagacagtgc
    13141 catctcacca tctccagctt cgccgacata agtgattgga taataagaac caaggacagt
    13201 tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa aaactttata
    13261 catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg acagttgttc
    13321 cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa taaagaagga
    13381 gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg atccctttgg
    13441 ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg ttgtctataa
    13501 ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat cctttgtgac
    13561 ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag atcattttta
    13621 aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc atatggccgt
    13681 atcttatttt taagatgact agataatcat tgatatggct aatttctttt tcaagaggaa
    13741 tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca tgggtcatag
    13801 tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc aaactgttat
    13861 aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt gctaaatggt
    13921 attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt ttattcatgc
    13981 tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag ccaaggttcc
    14041 ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg acctttctct
    14101 ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact ccaagttcca
    14161 ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt gctaagtcga
    14221 tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca gaatctacct
    14281 gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta ttgatcagct
    14341 cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac gtactaattt
    14401 ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt tcttctagga
    14461 tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc cacatacttt
    14521 ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata tttgcgatcg
    14581 tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc aaagggataa
    14641 cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg cttaaatttt
    14701 ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat attctgctgg
    14761 gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt ttgaatattt
    14821 ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt acactcgact
    14881 acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc atcaatagtt
    14941 acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc taaagcaccg
    15001 tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc atttgtttct
    15061 aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac aattcggtga
    15121 acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt tggtttacga
    15181 ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt ctctgaacca
    15241 gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt gctgacaaat
    15301 aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac gaaaagctcg
    15361 ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc tcgctttgct
    15421 tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat catatccact
    15481 ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat gtagccattt
    15541 cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc agtatgcttt
    15601 ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg gtcaggtgct
    15661 tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag ggctaaaaaa
    15721 aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta tcttatcaga
    15781 taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa acttaaaaaa
    15841 atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa aaaatcaatg
    15901 ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga taaaaaatga
    15961 aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc attctattaa
    16021 gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga atcgctatgc
    16081 cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg gaattagaaa
    16141 aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat caagtagcac
    16201 aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct atcgatggtt
    16261 cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg atcgcctatg
    16321 atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat aattttggag
    16381 taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa ggaaaaggac
    16441 cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta atcaattaca
    16501 acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc gtgccatctg
    16561 gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg gcgcaagcaa
    16621 ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct gttttgtcgc
    16681 catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg tatgggagtg
    16741 aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc gttaaatcga
    16801 tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta gcaaaaaata
    16861 cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat gatactgaaa
    16921 cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta tctgtcgata
    16981 aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc gatctgaaaa
    17041 attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga taagaattat
    17101 ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa tccgagcgtt
    17161 agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg gagaaaacgg
    17221 agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg gctcctatga
    17281 cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag atagtgaaga
    17341 aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt caataaaaga
    17401 gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg aactgacaga
    17461 aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga atcttctagt
    17521 atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat tatcaaaatc
    17581 cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag aaagcgcgaa
    17641 tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca ttatttcaca
    17701 taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg atggacagac
    17761 gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg gaatggtggg
    17821 cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag tttattttaa
    17881 agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa acgaccacat
    17941 caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg gagcgggcag
    18001 aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat ccggacgtat
    18061 attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg caaacggact
    18121 cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg atattcggga
    18181 aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag ataaagaaaa
    18241 agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa actccatcca
    18301 tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat ccaagtggct
    18361 gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag atgttggggc
    18421 aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag ctgtctgtct
    18481 tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata cgatgaacga
    18541 aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct tgatgaaatt
    18601 aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg gataaaaaag
    18661 agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac agcatggtga
    18721 tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt ttctggcgtc
    18781 cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta gctgcaggga
    18841 tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg atggcgtttg
    18901 ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg atgacagtta
    18961 tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg gtagcttatg
    19021 cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt ggactgacac
    19081 aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa aaaatgtcta
    19141 cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca ttgattttag
    19201 gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat cgtcggaaaa
    19261 atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta tttacagcag
    19321 tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg attattttga
    19381 tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca gtagcaggtc
    19441 gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg atcaaaacaa
    19501 agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta gctggattag
    19561 ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg gaactggatg
    19621 caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga acaatcatgg
    19681 gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg attttaggcg
    19741 tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc gttgtgctag
    19801 acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca gaaaaaagac
    19861 tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca ttgtttcgtt
    19921 tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc tttttgctct
    19981 tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc ttgattaaat
    20041 taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat gaaagtattt
    20101 gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg ttctgaatgt
    20161 gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc tgccaacatg
    20221 cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta tttctatatc
    20281 atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa aaagcgtgga
    20341 ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat agagaagttg
    20401 gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg acatgctaac
    20461 tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt tgtgattgcc
    20521 ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc agatgcaaca
    20581 aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg ctttgggact
    20641 ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa gccgatcatc
    20701 gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt tggtgcgaca
    20761 atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga aacaaaagtc
    20821 gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca aaaaggcgaa
    20881 aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt gaaagataca
    20941 ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg aagagatctg
    21001 gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa tggagacaca
    21061 atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca ttaagtaata
    21121 agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt attatcgcag
    21181 atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt tttgtcacag
    21241 tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta aaatcaaaag
    21301 aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa aaacaaggac
    21361 cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga aggatcaatc
    21421 ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg aatcgttcca
    21481 ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc aaacgtggca
    21541 gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga atcgattggc
    21601 ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa ataagagaag
    21661 caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg ccagatgagc
    21721 ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg gaagaaaaag
    21781 aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat ttggaaagta
    21841 aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt cagcaagcaa
    21901 tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta gagtcacctt
    21961 ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt gggataccga
    22021 tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa aataaattga
    22081 aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg agtcaaaaac
    22141 gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt gaagacgatg
    22201 tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga acgattgatc
    22261 cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca acgaaaatcg
    22321 gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa gaaatgatgg
    22381 agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat gaagcaattg
    22441 atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg ataagcgctt
    22501 ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac tatgcctgga
    22561 ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta gatatgggtc
    22621 ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga atcaattaca
    22681 gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt ctagaaaagt
    22741 ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct tggatttaaa
    22801 tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact aagaaaaaac
    22861 cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa caaaactgta
    22921 taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa aatagaaaaa
    22981 atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat gctgttaata
    23041 tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt gtatttctca
    23101 taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt tattcactgt
    23161 ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg acaaatggca
    23221 aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc agatttccca
    23281 gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt acctgattta
    23341 tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt attagaccaa
    23401 cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac tgctggacaa
    23461 gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct tcttccaggt
    23521 tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc ttttttatgg
    23581 tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct accaccacaa
    23641 attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt gaagaaacga
    23701 aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca aggtgatttt
    23761 tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta tatccaaaac
    23821 aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac acttgcacaa
    23881 aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc gcttgcagta
    23941 tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc agtcctaatc
    24001 gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc aacccgctac
    24061 cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg cttccgtaaa
    24121 tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat cgaagcaact
    24181 aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca aaaggtttca
    24241 gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca aattgcaatt
    24301 tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa gcaatcacag
    24361 atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt gaagatgttg
    24421 ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc ggagaagacc
    24481 gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc ggtcttgcgt
    24541 tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc gaagcaatgg
    24601 acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact agaaatctac
    24661 ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg cattcggata
    24721 atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct tccaaccctt
    24781 atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt gttttcctag
    24841 aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct tacgaagttc
    24901 ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc acatacggag
    24961 caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat atatctgttg
    25021 aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt caatccgtgg
    25081 aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc gttgccgcgc
    25141 aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca atcggacgag
    25201 tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg ttgccaaatg
    25261 caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag aagggacgaa
    25321 acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc gcagaaggtg
    25381 aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat acattactag
    25441 aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga acggttaaaa
    25501 atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta gaaatcgatg
    25561 caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc caaactccag
    25621 ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca agcgaaggag
    25681 tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa atcgtcaaat
    25741 ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa gttcaaaatg
    25801 acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac gtgattgttc
    25861 cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct cctggacaca
    25921 atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat
    25981 caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg gctatgccat
    26041 ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact gcaactggaa
    26101 aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg
    26161 caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct cctgcagcag
    26221 aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac ttggaagaac
    26281 gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac agtaagcata
    26341 cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg gataatcgga
    26401 aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg ccatatgtcg
    26461 tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct atcgacgatg
    26521 caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact gatacggatc
    26581 acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt gctattgcag
    26641 acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca gaagatatgc
    26701 gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg tttacaccag
    26761 tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa caaccaatcg
    26821 taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc
    26881 accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa cgtcttttag
    26941 ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg agattttgct
    27001 gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc tgccatccgc
    27061 gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg cggtgtttgt
    27121 ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca ttatcaagag
    27181 tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa
    27241 acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt tggtttccta
    27301 ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca
    27361 ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc aatcatcgca
    27421 acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt gttagattct
    27481 actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg tggcggtgtg
    27541 attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac gattcttgaa
    27601 ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt agaagatgat
    27661 ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac
    27721 ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt tactgctgat
    27781 tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt agaacaagcg
    27841 ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg tacgaacgta
    27901 cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca caaagccagt
    27961 tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt agattataaa
    28021 gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat gacgatcaaa
    28081 gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc agggaacggt
    28141 cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac gatcgaagat
    28201 aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta
    28261 gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat tcatcctcat
    28321 ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt accaatccat
    28381 atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta
    28441 gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg gtttattacc
    28501 acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg caagaatatc
    28561 agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg aagatgtggg
    28621 aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg aagacttatc
    28681 agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga cgggaatttg
    28741 aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca
    28801 aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata tcaagtcctg
    28861 gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa
    28921 atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt ttctaatcga
    28981 aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag ggtgtaatca
    29041 attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca ccttgaattt
    29101 tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg gcgttggaaa
    29161 gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa aaggaatagg
    29221 cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat cggaaggctt
    29281 acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag aaattgcgga
    29341 aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg caatcttgaa
    29401 aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag cgggaaatgt
    29461 cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt tgacatttga
    29521 aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg cagacctttt
    29581 gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa
    29641 agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa aagtctgcta
    29701 taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa ggagttagtt
    29761 acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga
    29821 aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc acatacgcaa
    29881 ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta
    29941 gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga tactccagga
    30001 cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg
    30061 gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa aaaagcactg
    30121 gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc tgctcgtcct
    30181 gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga tgatgatcag
    30241 ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt gtcagatgat
    30301 ccagcagatc aagaaccaac catggcacct atttttgata caattattga aaaaatccct
    30361 gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact tgactacaac
    30421 gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa agtaggagac
    30481 caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac gaaattattt
    30541 ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga cttgattgct
    30601 gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat
    30661 gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt agttaataat
    30721 tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga agaacgtttg
    30781 atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc tccagatgca
    30841 tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa catgcgccgt
    30901 gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat cgacggtgta
    30961 aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat gggtagtgtt
    31021 atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac aggaaacgga
    31081 caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc aactgaattc
    31141 ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata tctgccaatg
    31201 cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa
    31261 gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt
    31321 acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga tttgactgtc
    31381 aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga ccaaacatct
    31441 gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt gaatgatgat
    31501 gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt agagaaaaat
    31561 gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag agagaaacca
    31621 gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt ggagaagtca
    31681 tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa
    31741 aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga aaggagatca
    31801 acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg
    31861 acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa gaaattatcc
    31921 agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt gggaaatcaa
    31981 tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg tcccctttaa
    32041 tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc gccactttta
    32101 tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc gatagacagc
    32161 agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt atccaagcaa
    32221 tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc tctcagtggg
    32281 ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa ctgccaaatc
    32341 gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct gacataaaaa
    32401 gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga gaaaatctac
    32461 gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat ttaaagatca
    32521 acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac cgtctctatc
    32581 atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta aatgagaatg
    32641 agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt gtagcgacaa
    32701 atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat tatcaaattc
    32761 ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat ggtctttcca
    32821 gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt tttatccagc
    32881 agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa gcgatgacag
    32941 aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt ggcgagacaa
    33001 gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta gaagtaacga
    33061 cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa
    33121 tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta ggttttggac
    33181 atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg
    33241 aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct gttttgaaag
    33301 taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga aaggaaccca
    33361 agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg ctgcgggaac
    33421 tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt tcagattcca
    33481 cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt cagataaaag
    33541 gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg ataaaaaatg
    33601 cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa taaaaacaac
    33661 tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat taaggaatta
    33721 gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa
    33781 tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc tctgtatctc
    33841 acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt cccggcaagt
    33901 cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga tgttaagtga
    33961 gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt atgataaaaa
    34021 agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca tctattagac
    34081 tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat ggtatatagt
    34141 tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt cattaaccag
    34201 agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat gaaaacagat
    34261 gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat tttgctgctg
    34321 attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca aattgcagga
    34381 ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct gtctctgact
    34441 ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa acgtccactt
    34501 gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg taatgcggca
    34561 gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta tgtgtatact
    34621 ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct gatcaatatc
    34681 acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt tgctatttac
    34741 caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg ctactatgcc
    34801 atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa aaaaggattt
    34861 ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact gggagtgatt
    34921 atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt ccttgtaggt
    34981 atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc aatgtttttg
    35041 atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac agggatcacg
    35101 ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg tgtcggcttt
    35161 gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata aaaaaagatg
    35221 taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt
    35281 atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag aagaagatga
    35341 atattccgta caccgtttca aagcggatga agcatattta gtaggacaag gaaaaaaacc
    35401 gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat gcggagctga
    35461 cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga
    35521 agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt ttggagataa
    35581 aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag gcacagatgg
    35641 accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt atccgattat
    35701 gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg atgaaaagag
    35761 tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga
    35821 agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga ttctcgggga
    35881 tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc gccgtcacca
    35941 aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg
    36001 ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt
    36061 tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc aagtggaaca
    36121 tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt tgattgctca
    36181 aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta aattaaatgg
    36241 ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt taccagacac
    36301 agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg atgtcggaaa
    36361 tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa aagtatgtac
    36421 gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga aagaatttag
    36481 aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac atcctgaatt
    36541 ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat ttgaatttcc
    36601 tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg
    36661 ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga
    36721 tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga agggtgccga
    36781 tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata cgacatttag
    36841 agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca agcagattgc
    36901 acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg ggggagcaac
    36961 atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga gaaaaattcg
    37021 ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta
    37081 ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag cgcaaggaat
    37141 cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa aaagtatcca
    37201 agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg gcgatatcaa
    37261 cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa aggaactaga
    37321 acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta aaccgcaggc
    37381 agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc atctgcatac
    37441 tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag caggagtaga
    37501 catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta gcatgagcag
    37561 cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg aaaatgcaca
    37621 gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg aaaacggctt
    37681 gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt attcgaatct
    37741 acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca aacaaatgta
    37801 tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat ctaaagtagt
    37861 tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata tttatgaaaa
    37921 aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag aactagggca
    37981 acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca gacctgcatt
    38041 gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt
    38101 ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt tgatgtatcc
    38161 gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta cattactgga
    38221 cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg
    38281 cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg aagggaatcg
    38341 cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag ataattcgat
    38401 catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac aaattggagc
    38461 aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg
    38521 tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag cacgttttga
    38581 cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg gtgacctttt
    38641 gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag actgggtttg
    38701 ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt cttgtttccg
    38761 ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat gacaacgtgg
    38821 aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc gatcgatcag
    38881 ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg ttttgaaaca
    38941 agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca agacagcaga
    39001 gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg
    39061 atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac ttttgagtac
    39121 catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg tccgctaatt
    39181 gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa cggcctattt
    39241 tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca agtgtttggt
    39301 gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat caatcgcgtt
    39361 aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga acttcccata
    39421 tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga ttttttgaca
    39481 aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc gctaggcagt
    39541 gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt aatcagtaaa
    39601 caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc tactttttcg
    39661 tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc gggaacggat
    39721 tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga agaaaacgga
    39781 gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg atcaatgcct
    39841 ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg aacataaaca
    39901 gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga
    39961 agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg aaaaaaaacg
    40021 agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag ctttccgata
    40081 tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg ccgaagaagt
    40141 ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa aacactttgg
    40201 atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca aaacgacgcg
    40261 ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt
    40321 tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa gagacattgg
    40381 aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa cgttcttacc
    40441 gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta ttaaaagacg
    40501 aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat ctttaaaaat
    40561 ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt ttgatcattg
    40621 tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga gtagatgcgg
    40681 ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga
    40741 ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata ttttgatgga
    40801 gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc agtatctaga
    40861 ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat catcaaagaa
    40921 aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga tgcggacaaa
    40981 gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct tgatccgcca
    41041 tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg acttttttct
    41101 aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga aactgtggct
    41161 ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat ctatagaaaa
    41221 gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt acaaaaggtc
    41281 acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt ggtgttttta
    41341 tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg atcacaaaag
    41401 ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa
    41461 cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc aaagactttg
    41521 aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg gaaactgtct
    41581 tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa gaagtactgc
    41641 actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct ttagctggaa
    41701 agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat catatttgga
    41761 atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc accgggagcc
    41821 acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg ttctgggtct
    41881 ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa
    41941 atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc gacaaatgaa
    42001 gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc aatcgaacaa
    42061 gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata tgtcatgagt
    42121 gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt aacaaaagta
    42181 gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag ccaaaaagtt
    42241 ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc ttctggtgat
    42301 ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac tgaccatacc
    42361 gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg gccatcagcg
    42421 ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt gcgacatggt
    42481 aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg aatcggagga
    42541 atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt tgcaccagat
    42601 gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa
    42661 gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta
    42721 caagaagcgc ttgactatct agagaaaatg aaat
//
LOCUS       DASJTC010000010        42142 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.010, whole genome shotgun sequence.
ACCESSION   DASJTC010000010 DASJTC010000000
VERSION     DASJTC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.010"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..590)
                     /locus_tag="U4B19_000520"
     CDS             complement(105..590)
                     /locus_tag="U4B19_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEN2076287.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(592..1356)
                     /locus_tag="U4B19_000521"
                     /pseudo
     CDS             complement(592..1356)
                     /locus_tag="U4B19_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1526..2719)
                     /locus_tag="U4B19_000522"
     CDS             complement(1526..2719)
                     /locus_tag="U4B19_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2076288.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(2803..3003)
                     /locus_tag="U4B19_000523"
     CDS             complement(2803..3003)
                     /locus_tag="U4B19_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEN2076289.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3427..3660)
                     /locus_tag="U4B19_000524"
     CDS             complement(3427..3660)
                     /locus_tag="U4B19_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2076290.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(3667..4098)
                     /locus_tag="U4B19_000525"
     CDS             complement(3667..4098)
                     /locus_tag="U4B19_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2076291.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
     gene            4407..5746
                     /locus_tag="U4B19_000526"
     CDS             join(4407..4848,4848..5746)
                     /locus_tag="U4B19_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEN2076292.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
     gene            5866..6039
                     /locus_tag="U4B19_000527"
     CDS             5866..6039
                     /locus_tag="U4B19_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076293.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
     gene            complement(6105..6713)
                     /locus_tag="U4B19_000528"
     CDS             complement(6105..6713)
                     /locus_tag="U4B19_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HEN2076294.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(6719..7747)
                     /gene="vanB"
                     /locus_tag="U4B19_000529"
     CDS             complement(6719..7747)
                     /gene="vanB"
                     /locus_tag="U4B19_000529"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HEN2076295.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            complement(7740..8711)
                     /locus_tag="U4B19_000530"
     CDS             complement(7740..8711)
                     /locus_tag="U4B19_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HEN2076296.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            complement(8708..9415)
                     /locus_tag="U4B19_000531"
     CDS             complement(8708..9415)
                     /locus_tag="U4B19_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEN2076297.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            complement(9433..10239)
                     /locus_tag="U4B19_000532"
     CDS             complement(9433..10239)
                     /locus_tag="U4B19_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HEN2076298.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            10518..11819
                     /locus_tag="U4B19_000533"
     CDS             10518..11819
                     /locus_tag="U4B19_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2076299.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(11910..13271)
                     /gene="vanS"
                     /locus_tag="U4B19_000534"
     CDS             complement(11910..13271)
                     /gene="vanS"
                     /locus_tag="U4B19_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN2076300.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(13274..13933)
                     /gene="vanR"
                     /locus_tag="U4B19_000535"
     CDS             complement(13274..13933)
                     /gene="vanR"
                     /locus_tag="U4B19_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HEN2076301.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            complement(14551..14718)
                     /locus_tag="U4B19_000536"
     CDS             complement(14551..14718)
                     /locus_tag="U4B19_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076302.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            complement(14876..15061)
                     /locus_tag="U4B19_000537"
     CDS             complement(14876..15061)
                     /locus_tag="U4B19_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2076303.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            15206..15576
                     /locus_tag="U4B19_000538"
                     /pseudo
     CDS             15206..15576
                     /locus_tag="U4B19_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            15834..16163
                     /gene="mobC"
                     /locus_tag="U4B19_000539"
     CDS             15834..16163
                     /gene="mobC"
                     /locus_tag="U4B19_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEN2076304.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            16124..17452
                     /locus_tag="U4B19_000540"
     CDS             16124..17452
                     /locus_tag="U4B19_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN2076305.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            17434..17820
                     /locus_tag="U4B19_000541"
     CDS             17434..17820
                     /locus_tag="U4B19_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HEN2076306.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            18003..18239
                     /locus_tag="U4B19_000542"
     CDS             18003..18239
                     /locus_tag="U4B19_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2076307.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(18539..18742)
                     /locus_tag="U4B19_000543"
     CDS             complement(18539..18742)
                     /locus_tag="U4B19_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HEN2076308.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(18746..19690)
                     /locus_tag="U4B19_000544"
     CDS             complement(18746..19690)
                     /locus_tag="U4B19_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076309.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            complement(19691..23611)
                     /locus_tag="U4B19_000545"
     CDS             complement(19691..23611)
                     /locus_tag="U4B19_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HEN2076310.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            complement(23760..25841)
                     /locus_tag="U4B19_000546"
     CDS             complement(23760..25841)
                     /locus_tag="U4B19_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEN2076311.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            complement(25838..27067)
                     /locus_tag="U4B19_000547"
     CDS             complement(25838..27067)
                     /locus_tag="U4B19_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HEN2076312.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            complement(27057..27308)
                     /locus_tag="U4B19_000548"
     CDS             complement(27057..27308)
                     /locus_tag="U4B19_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HEN2076313.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            complement(27331..29322)
                     /locus_tag="U4B19_000549"
     CDS             complement(27331..29322)
                     /locus_tag="U4B19_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN2076314.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            complement(29354..31753)
                     /locus_tag="U4B19_000550"
     CDS             complement(29354..31753)
                     /locus_tag="U4B19_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEN2076315.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            complement(31683..32105)
                     /locus_tag="U4B19_000551"
     CDS             complement(31683..32105)
                     /locus_tag="U4B19_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HEN2076316.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            complement(32119..32655)
                     /locus_tag="U4B19_000552"
     CDS             complement(32119..32655)
                     /locus_tag="U4B19_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEN2076317.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            complement(32692..33555)
                     /locus_tag="U4B19_000553"
     CDS             complement(32692..33555)
                     /locus_tag="U4B19_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076318.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            complement(33647..33862)
                     /locus_tag="U4B19_000554"
     CDS             complement(33647..33862)
                     /locus_tag="U4B19_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HEN2076319.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            complement(33939..35747)
                     /locus_tag="U4B19_000555"
     CDS             complement(33939..35747)
                     /locus_tag="U4B19_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEN2076320.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            complement(35744..36220)
                     /locus_tag="U4B19_000556"
     CDS             complement(35744..36220)
                     /locus_tag="U4B19_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEN2076321.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            complement(36295..36507)
                     /locus_tag="U4B19_000557"
     CDS             complement(36295..36507)
                     /locus_tag="U4B19_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEN2076322.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            complement(36529..37494)
                     /locus_tag="U4B19_000558"
     CDS             complement(36529..37494)
                     /locus_tag="U4B19_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2076323.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            complement(37540..37728)
                     /locus_tag="U4B19_000559"
                     /pseudo
     CDS             complement(37540..37728)
                     /locus_tag="U4B19_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(37985..38659)
                     /locus_tag="U4B19_000560"
                     /pseudo
     CDS             complement(37985..38659)
                     /locus_tag="U4B19_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
     gene            complement(38692..39543)
                     /locus_tag="U4B19_000561"
     CDS             complement(38692..39543)
                     /locus_tag="U4B19_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEN2076324.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            complement(39699..39944)
                     /locus_tag="U4B19_000562"
     CDS             complement(39699..39944)
                     /locus_tag="U4B19_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2076325.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            40049..40822
                     /locus_tag="U4B19_000563"
     CDS             40049..40822
                     /locus_tag="U4B19_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076326.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(40875..41189)
                     /locus_tag="U4B19_000564"
     CDS             complement(40875..41189)
                     /locus_tag="U4B19_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076327.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
BASE COUNT        10173 a        10654 c         9842 g        11473 t
ORIGIN      
        1 cagtttgatg aattaactga taaaaaaata tctactattt aaagatacag ggataagtaa
       61 gtaaaaaaat aacatgtcat tagatagtag acagttccaa ctctttattt accttttttg
      121 tatgctgcac tgtcaatcca agttcctaaa ttgttgtatc catcatcact tatataatca
      181 tattggccat caataatttc gtcaaaccac aaatattctc catctggttt ttgcccaatt
      241 attttaatta ctgattgact agtagttgtc atctggttaa aatcttttat tttacctata
      301 ttaacggcca aaatagcatt tcctcgctta taactctcct ggatttcata tttaacatat
      361 tctcgatcaa gagtttcttt acctatcagc accacagtca ctgatgttcc aattaattga
      421 ttatctatcc agttttttat agcaatatct ccattacgtt taactttctc aaattcagcc
      481 ttatctacaa aaccagcttc ctttgttcct cgcaaagtcc agctgtttct aactaccatt
      541 gctcttgtta catctgcata atggaaacta aaaaaaaccc ttctcaccat attaatcctc
      601 ctgaatacta tttattaaac tgacaatttg ttcaactttt ttatctactt ctccttcagt
      661 atctaatttt tctagcgctt gaaatttact ttttaaggaa tcatcaaaat aatcactgaa
      721 attctcttga atcttatcaa acaaaacctt acttgcacct ttggtaaatc caatcggaac
      781 tactatattt tcattttgaa cagataattc aaattcttca atcattccat ctgctgtaac
      841 tatttcacct gattcaagtt tgtttccaaa aacaaaaata ctaatcccag actctgcaat
      901 aatgttttct cgatatcttc tccactcatg aggcatatca aggggaagtg gtcttaaaga
      961 aagtacgtct tgtatgttat tttttcttaa atttcttctc tgctttagaa ctcctgaaac
     1021 aatactatta ccaattccaa gtccgtaacc tgatactatt ctataaccgg attcagaaag
     1081 cctatatgct aatttctcga caaattttaa tcctttcgat tcagatgtaa atggagtata
     1141 atcaacagca cttcccgaaa taaaaatagt ttttctcaaa tacttttttc taatactttt
     1201 caaaatatca gtaatctcag aataatcatc tacttcaaca acgtttatcg aaaatctgga
     1261 caaatcattg atttgtagtc ttctttttgt cagttcataa ttatatctct cttgatcaaa
     1321 aaaatcctca gaatcgacaa aattttcctt taaaattata tgggatttta gattatcttt
     1381 ttagaagttc ttgaatgtcg gtatagcccg tgtttccggg cttttccggc atttttgata
     1441 ttgttagata cttacaaatg ttcttattac ctctcaccat ttccccctca aaagtagtaa
     1501 atggagtagt aaaaccggcc ttgtgtcagg ctgccagccg ttccatttct gcccttgcgg
     1561 aatcgaatgt ggcgtgtgca taatagttca gcgtcatggt gatattggaa tgtcccataa
     1621 tgtactgcaa ggctttgggg ttcattcccg cattggctaa ccgggtgcag aatgtatgac
     1681 gcatagcatg gggcgtcgtc gttttcggca acgcttcttc atggctcttg ttgtacttct
     1741 tcacaagcct gccaaacatc ccgccataat ctaccgccgt catgggatag ccgttctgtt
     1801 tgagaaacag gaaattggca tacccgtcaa taatgaacgg ctttgcacct ttgcggtttt
     1861 ttatcactcg ctggaacgcc tgatacactt cctcattcat ggggattttt ctgacgccgc
     1921 tttgggtctt gggttcgtca atatagtaac cgacgccggt atttctcaaa agctggtggc
     1981 tcacgatgat tacccggttt tcaaaatcca aatctttgtc cgtcagtccg cacagttccg
     2041 aaatgcgaag ccctgtcccc agcagaataa cgattgcgtc ataatgcttt tggtaaacct
     2101 tatcaccttg aataaaggac agaaaacttt cttcctgtgc tggtgtaaga ggcacctttg
     2161 gttccgtatc gtcgtccaga acgtcactaa gctggaagtc aaacgggttt tttcggatgc
     2221 aatcgtcctg tatggcggtg taaaaagccg ctttcaggga acgcttgtcg ttattgatgg
     2281 tcttataaga aaggcccttt tctttcatcc gaagcgccca ttcctttgcg tcggacagct
     2341 tcacactgtc aatcgggcag ctccccagcc gatcttgttc cagaatacgc atcagccgtt
     2401 cccggcccag tttggtgttg tgcttcacat tgccccggtg cctgatctgc ttggcgtaga
     2461 gctggcagac ggtcattttc ttgccgatgg tgtctatccc gtcgtcaagg tctttctgga
     2521 tttctttctc cttttcccgg agtgaaatat cctcccgttt acctgccggg gtcttatccg
     2581 taggcactaa cttccatgag taaacaaact gcggttttcc aaaggtatca acatatttgt
     2641 aagcatatcg tccgtctttt ctctggctct ctccagaccg caaaacgcga tttttgctgt
     2701 ctcgtctttt ttctgacatc tcgtcatgct cctttccatg acggaaagag ccgcatatat
     2761 gcttataaat tattataaca tatctgcggc cctttttcac tattagattg tgtccaaagt
     2821 gtcaattatt ttctcaaact gcttgcgttt gatctggatg cggttgccat tcataatgac
     2881 ccagccagag gccggatttt cctcggccag cttacgcaat ttgttttctc cgatacggaa
     2941 atatttagac gcttcctcaa tcgtcagcgt gtatttttcc caaataggca catcatggtt
     3001 cataatgcag ccccctttca caaattcaaa ggtttgtgtg atttaggggc atagacggac
     3061 ggctgctggc acagctccac gggactttca cccccgcgtg gttctcacgc ggccctaccc
     3121 attgcctgcg acgcttttaa cgctcggact gtggctataa gggagtatca tagtcctgcc
     3181 tgtacgtcgt cgcccgcaag ccgccacgct cacattgcgg cgtggtgttc ctcgctccgc
     3241 ttttcccgta gggaaaagta tcatggcgca ccagccgtcc ggctcggcac agacagaatg
     3301 tgtccgtatg ccctatgatg cgccgccgtt atcttgcggg catatttatc aaggtgcgat
     3361 caggccaccg ggaaaacacc gaaacgggga aagggtaagc cgtttcatgc tgccggggcc
     3421 ggttcgtcag ttcgtgacga tagcgtggat gagcttgatt tccagacggc gcttcatggc
     3481 ctcgtccaga cacacatgcg gatagccgtt ttcgtcatag agcgtccggg tacacagctt
     3541 gttcatgtaa ccgctgtaat actgcaaaac gcgctctacg gctccggcat ccccagcgcg
     3601 ggcggcctcg atcaccgaaa ggggcaaaag ctcaatgccc ttgtagttcg ggttcttcat
     3661 ccgcgtctaa cctcccttcg gcatcaacgc ctttagctgt ttccgcagct ccgataaggt
     3721 atttgtcctg tgccgctgaa cggcactccg ggacattccc acaaggccgc cgatctcacc
     3781 atctgccata tccagaacac agtgcaggat taaaatactc tgctccatgc tgggcagcgc
     3841 ggcaaaggct tcggcaacaa gctcattgtt gatatgtagg tcatagccat gcgacgaaaa
     3901 cgtaaagctg tcgctggggt agtgatctac tgtacagagc ttgtccaaat ctgcctgtga
     3961 caaagagctt agtgacactt cacggtcacg gcggcgcttc atttcagcca agtagctttt
     4021 ggcctcgtta cgcagtaccg tcttgcaaaa agcatcgaac atgcaccgcg cttcatagtc
     4081 gcgggggata gcatccatct tctcaccccc tttctgtggg ggacttaggt ggttctctcc
     4141 ctttccgcta acaagacttt gacaaggccg ccagctaccc gctaaatacc tcctttttga
     4201 aaatttctca aaaaattttt ttggggacag ccgggccgca aaagacgaca aaaatcgccc
     4261 ggcaggtcgc aaacctaccg ggcgagaaaa tgctttgcta tgctgctgat ttacatggta
     4321 tagtacatac tcataaccgg aatagccctg tggtgatgtg caccccaaaa gttagactag
     4381 aaatctaatt aaaaggggtg ctttttttgc ggaaatatac atttgttttt aagaaaaaag
     4441 tagtttcaga ctatttaaat aacgaaggcg gctacaaata tcttgcacat aaatatcaaa
     4501 taaatcgtac actggttaga cattgggtaa ggatttataa ctatcatggt tgggaaggct
     4561 tggttggagg tggcaaaagc tacactacaa aatttaaact tgatgttata gaatatatgg
     4621 aaacaaatgg tctttctatc caagaaactg ctaaaaaatt taatatcggt tcaaatagaa
     4681 ctctaagtaa atggatagag caatatgaag aaggcggtgc ttcttcactt gagagccaaa
     4741 aaaggggcag aaaaattagt atgaattcca agctaaacat tcctaaaaaa cttaaagatg
     4801 agtctcttga agaagaagtt attcgtttaa gagcagagaa cgcatattta aaaaagttag
     4861 aaaccttgat tcaagaacaa gatttgagca agaagaaatc aaggttaaaa caatccttca
     4921 gctcaaaaaa gaatttaaat tgaatctatt actttcaatt gcacaattag cgaagtcaac
     4981 ttattattac tgggtaaaaa aattagataa gccagataaa tatagcaaga taaagcaaga
     5041 aattacagcg attgtaaaag aatcaagaaa ctcttatggt tatcgtagag tcactttagc
     5101 gttaaagatg aaaggatata caatcaatca taaaacagtt agaaaattaa tgtcccaaat
     5161 gggacttacc tgtcaaatcc gaataaagcg atataaatct tataaaggaa cagtaggaaa
     5221 aattgccaag aatgtgttaa aacgaaattt ttcagtagat acacccaata aaaaatgggt
     5281 gacagacgtc actgaattca agataaaagg aagaaaaatc tatctatctc ctatacttga
     5341 tttatttaac ggagaaataa ttagttatag tatatcaact agtccaacat ataaactgat
     5401 tgaagaaatg cttcaacaag caattaaaaa aaagggaact gagggttcac tgattctaca
     5461 ttcagatcaa ggatggcagt atcagatgcc acaatatcaa aaaaaattaa aagagaataa
     5521 cattattcaa agcatgtcta gaaaaggaaa ttgcttagat aattctgtaa tagaaaattt
     5581 ttttggtgtg cttaaatcag agttttttta tcgagaaaaa ttccgatcaa ttgagatatt
     5641 tcaaagtaaa ttaaatgagt atattaggtg gtataataac aaaaggataa aactaaagtt
     5701 aaatggacta tctccagtag aatatcggaa acagtccatt aaatagttca acttttgggg
     5761 ttcagttcat ggaggggcaa ggcttttttt gagtggtgga agtggattag agcatggcaa
     5821 tagcaaaaca gcatagcggc atcctcctat gggccgccac accttttgct gccagaaaag
     5881 aacggcggcc ttgatttctc aaaaccgccg ccagcaaaat acaaaatggg cgtgggaaag
     5941 gtctgcccaa cagcggtttt tttcttttct gccgctgggc agattgaata ctataatttg
     6001 caatgtatta caaaagatgc tttaaccact ttccagtaaa agattgtcca tgaccagcat
     6061 agattttatc aaatccaaaa tctgatacaa aaccaatact ttcattatga aacggcaaaa
     6121 ttaaaatagg tatcggggta tggctcatca atcaacttat agtgccacca ttcaaagcga
     6181 taagactgaa atccgctgct ttccatgatt ttacgcaagc atcttcgatt ttgtgcctct
     6241 gcatccccta tcccttttgc tgtatgatgc gaccgttcgt ccataaaatc gaagttactt
     6301 cccattgaaa caagttcccc tgtatccagg tggtagagcg taagatcaat tgcgcttcca
     6361 cggctatggc tggattgtga ggccacataa ccctttgtaa tcaactcggc tcgctctata
     6421 ttggggtaaa atttttcttt tgtgagattg tcctccggtt gcgctgccca acgcaggaag
     6481 cagtccaccg cagttcttgg ccgatatcca tcccataaaa gcaagccata gccaagcgca
     6541 gccgcatgga tttgtgcctc gcgcagagca aacgccacgg cctttgtgcc gatgatgcga
     6601 ttcacctcat acccatccac cggttttcct gtgaagttat cccatgtggc gtacttggca
     6661 tcccaacgga cgccatgcaa catttcatct aaaaacaaaa aaccattttc catacttatc
     6721 acctctttaa cgccaatgta atcaggctgt caatcagtgc aggaagcgtg attcctgcgg
     6781 cggccatcat acgtgggtag cggctgtacg atgtaaaacc gggcagggta ttgacctcat
     6841 ttagaacgat gccgccatcc tcctgcaaaa aaagatcaac acgggcaagc cctctgcatc
     6901 caagcacccg atatactttc tttgccgttt cctgcacccg atttcgttcc tcgaccggaa
     6961 tgtctgcggg aactgtaatc atcgcatttt ctgagccttt ttccggctcg ttttcctgat
     7021 ggatgcggaa gataccgtgg ctcagccgga tttgatccac ttcgccgaca atcaaatcat
     7081 cctcgttccc catgaccgca cacccgacct cacagcccga aatcgcttgc tcaattaaga
     7141 tttttccatc atattgtcct gccgcttcta tcgcagcgtt aagttcttcc gtaccgttta
     7201 ctttggttac gccaaaggac gaacctgacc gtgccggctt cacaaagaca gggtaggtaa
     7261 gcgcacccgc ctccggcttg tcacctttat caatcatttg aaattcggga acggcgatgc
     7321 ccgcattttt tgtaagaatg taggccagtg atttgtccat gcaagctgcg gagctttgaa
     7381 tatcacagcc cacatagggg ataccagaca atacaaacag cccctgtatc gcaccatcct
     7441 ccccgcattt gccatgcaaa accgggaaag ccacatcaat acgccgtgtt tcgtattcgc
     7501 tttctttcat gacaagcagc ccatgcgttt tcctatccgg ggagagtatg gcggggagac
     7561 tgtcggcttc ccattccgta catggcttct tgcatagctt ccatacaccg ttttttgtaa
     7621 ttccgatgta gtgcggatcg aatttttccg tatcaatgtt cgcagcaatt tctattgcgg
     7681 cttttaccga cacatcatgt tcctccgagc aaccgccgaa gatgattgcg acttttattc
     7741 tattcatgct gtaaactcct ttcaaaattg agacaattcc tgattgtttt ttctgtagta
     7801 tcctgcagca cacgctcagt gtagtacgcc gtatggggtg tgatgatcac attcggcatc
     7861 cttaaaagct gcgacaaaaa gggatggtca agcactttct gcgagcaatc ggtataaaca
     7921 aactgatcct cgccctccaa cacatccagt gccgcaccac ccagctttcc gctttccaac
     7981 gcctccacca gcgccccggt atcgacaagc gctccgcgcc cggtgttgat taaaaatgcg
     8041 ccttgcttca tctctccaat ttgcttctga ccgatcagat ggcgggtatc cgcacaaagc
     8101 ggcacatgga gcgtaacaat atcgctgttt tttagaagct catcaagctg gacataatct
     8161 gcatccattt ttcggctgtt atcataggcc agcacatggc atccaaatcc ccgcagcctt
     8221 ttgacgaccg cttgcccgat atggccggtt ccaataactc ccacagtcat atcccccagt
     8281 tctttccccc ggatacgatc caatctgaaa tcttgttgcg ccacggcgtg catggtgggt
     8341 tttgcacccc gtatggccat cagcatcagc atcaaagcat aatccgcaac gctgtccggc
     8401 gaatacgcaa ccgtgccaac cgagattccc attctctcgg cggcagtcgt atcaatgtga
     8461 tcgcagccga tgctgcgggt agaaatgtat tttaccccga cctttctcag cgcaagaatt
     8521 gtcgcctcgg aaacctcgga cttatggctt acgctaacgc attgattgcc agcggccaat
     8581 tttgcgttgt ctgccgatat cgcatcgcta atcagcgtag ggataatatg aaaatccggc
     8641 gataaagcgc ggaaagcatt tgcctcatcc tgttcgcatc caaaaacagt aatgcccaca
     8701 ctttttctca ttggttctcc tcctgaatat ccacactttc cgggagggga taacagattt
     8761 tgcatttgtt tgtataaagc agcttgcaat ctattatttc cccggtacct aaggcaactt
     8821 gcatccgttt aacctgggca tattcataaa tcccgccact ttcacggaca tactggatac
     8881 tgccatttgt aattttgtat aatgcttggg gctctttgtc ggcgaacacc tgaccgatga
     8941 ttttctcatc atctaaggtc acccatattt ggtaggtgca gtcggtatcg tttcgcactt
     9001 ttaaatcaat ccagccctct gaaatggttg catccacccc tttaatctcg tcactgtttg
     9061 gctctggaaa ctcctttact tcgtgacctc tgcgctggat aattgtcaat ggcgtatgca
     9121 ggaacaacca gaaagaaaag gtttcccccg gtcttatcaa taagccgttt aaggtctttg
     9181 ctgccagctt taagttgaac accttgtttt cctggtatat catgtcaaat ccggtattgt
     9241 ggttgtataa tgcgcaatcc gtttcaaaga gcaaatggga aagcgatttt tcccctatcg
     9301 tctgcgcata gcgacagccg tcaaatctca ttcccgcata aaagcacatt tttctctgcg
     9361 cccgtctcat tggaagcagg aacgggaaac gctgtgtcaa tctttttctg tccataacat
     9421 aacatccttt tctcattttt ctggttttaa atattcctca aggcaaaccc cttggttgta
     9481 catctctgtg gcagcttcga tgccaacata tcggtaatgc cacggctcgt tgctcacacc
     9541 ggttatctct gtcttgtctg gtgggtagcg acgaataaaa ccaaaacgat agctgttttc
     9601 atccagccat ctataaacct cgttgcctgt tgaatgaatt ccgtccgcat tgatatccac
     9661 agcaagacca agctgatgct cgctcgttcc cggcacggcc acccaagttt ctgcttccgc
     9721 tttagcctgt gcagaggtgt agcctttcgc cttatattcg gcaatttttt catccataat
     9781 ttcttgctgt ttttctgttg ttcggtatcc ggatgcgaca atcgggtaaa ctccatcagt
     9841 tcttgcggca tcaaacaaat cttgaagata gggagaaatc cgaatatcta tccgctcacc
     9901 atttgatagt tgctcaagct ccacatcgta ctgtgcgggg atggggttct gcctgttcac
     9961 taaaatcagg ctccattcct gttcatctcc ctgttctttg gcggtggcaa gagcgttttc
    10021 ctccaaattc tccactacgt cagtgctgct gcctgtatga gttgcaggga tgggcggtat
    10081 ctgtgttgcc tccaatacgg aagccagtct ccaccccaaa aacagcgtgc agactgtgaa
    10141 cgaaataatg aacgcacata gaaaagcccg cttttccacg gattgtttca tatgccgttt
    10201 gtgatgatcc gcattggaat gatagttgct tttttccatt tgatcgaatg cctcctttgc
    10261 aataaaacta cgatttgtgg ctggtacgat tgttattatg gcaagcgcat ctgtactttc
    10321 cctgtagcca gtctgtagtt ttcctgtagc agcaaaaaaa gaccggcagg gttatacctg
    10381 tcggtcaaaa tcggctctat aatttctagg actgatagaa aatcattcta tcggtccctt
    10441 ttgagcatga aaaaacaaaa catacagaag ctccgttaga attgaatcac cacaacacaa
    10501 tcaaaggagc gttctgtatg cctgatcttc atcatatcaa actactactt ggaatcaaag
    10561 ataaaaacat tttcatcaca aatgtggaat cgaagagcat taagaatatc aagagtcttg
    10621 ttgtttctgc gacgttaagt aaagaaattc gtcgctgtcc tttgtgtaaa cagatgaatc
    10681 atgaaggcat gatcgttaag aacggaaaga aaaaatcgct cattcaactt aataaatgtg
    10741 cgaaccaact gacttatctt gctttagcga aacaacgcta ccactgtcgt ggctgccaca
    10801 cctattttac ggcgaatacc tacattgtcg atagaaactg ctttatcgct aaacaagtgc
    10861 gctataaaat tttagaagag ctaactgaaa aacaagcaat gacgacaatt gctaagcatt
    10921 gcggcgtttc ttggtcgacc gtttctcgga cactcgcttc tctgatcccc atgacaaaag
    10981 taaaaagaaa ttggttaccg cgttgtttat taatggatga atttcgttca ctgaaaaatc
    11041 aagtcggtcc ctactccttt tcttgtatgg atggcgatac tggaaaatta ctggatattc
    11101 ttccttcacg gaagaaaaag gctctcgttt cttattttat gcaatttgaa cgacgcgcac
    11161 gattgaaggt aaaggtcatc gtgacggaca tgaatgtttc ttatgcctct ctgataaagg
    11221 aatgttttcc aaaggcaaaa ctcgttgtgg atcgttttca cattgtcaaa catctcattc
    11281 gtaaatttga ggatattcgc gtacgaatca tgaaaagctt tgatcgaaac gatccaacac
    11341 aagccaaata ttatcgacaa ttaaaggcgt tgtctcggct tctgatcaaa cgacaggaca
    11401 agcttgttta tgacaaatgg accaaatggc gcaattttgg ttgggcgtat ctcaccgaaa
    11461 gtgaagtggt ggatcgtctg ctttcgatct ctgacgaatt acgaatcgcc tatttttact
    11521 atcaagaaat tcttcaagcg tttcacgaca aagaggcgga cactttcttt aaacttgtaa
    11581 gaacgatgcc caattccgtt ccaaaagaac tgcattctat aaaaaaagct tttataaact
    11641 atgaatccgg cattcgttta gccctcgaat tgccttattc aaatgccaaa atagaaaatc
    11701 tgcataccca catcaaagca ttgaaacgag tcgcttatgg ctttcgaagc tttcggaaaa
    11761 tgaagaccag aattttctta ctaaataatc tgattaccta tgaatcaaaa aacatctaaa
    11821 gtagaaaagg aattctacct tagatgttct tatctcactc tatcagtcct atttgacaaa
    11881 gagcccaaaa tcatttaagt tttaaatatt tacaatgttg atgtgagcgg taaatccagc
    11941 cagaacaaaa cgccatccga ggtgttttcc agcgcatact ggaggctcat ggcatccagc
    12001 gttttttgta cgatggcaag tcctaaaccg cttcgcccac tttttctgct tcgcccactt
    12061 tttctgcttc gcgcctgatc aatgcgatag aatggggtga acagccttgg aagcgcagta
    12121 tcatcaatgt gaacgcccat gttcaaaaca aaaaggcggc atttttcagc accaggctca
    12181 ctccatatcc gtacctcgcc tccctgcggc gtgttctgaa ctgcattcaa tatgacattg
    12241 gatagtgcct tttggagcag cctcggatcg gacaggacaa tttgcccggc tggaatatct
    12301 gtgacgaacc gctggttgtt tgcctctgcc aaggtttgaa aatcgggcag caactcggca
    12361 accgtgcgcc ctatgtccaa cggttcagct atgggtacga ttctcccatc attcaggctg
    12421 acaagctcca gtatttcgga aatgattttg ccctgcctat ccatcatttt gatgcattcg
    12481 cgcagatact tagaatgatc tttgtagtca ccgatatttt caagcattcc ctccaacaga
    12541 acgcttgtag ccgcgatggg cgtttttagc tcatgagaag ctgccgcaaa gaaatatcgc
    12601 tgtgtttcct ccaactcatg ttcccttgcg atttcatcct ccagccttgc gatggtttct
    12661 ttcagcctga catacatgga atgcatatca tgagccagtg cgccaagctc atcctttcgc
    12721 tccagcggcg gcggtacatc tttcaggttt gccattttat tcgcactgtc cgctaaggct
    12781 ttgatcggcg ttgtcatttg ccgcgcaaag atatacgcgc ataaaaggct gaatacaacc
    12841 attatcgcaa tcataacaat tccccgaatt gtcagccctt gataaagcaa tcccacacct
    12901 gccttgcttt gagcaacaat cgaaatatta tcatctctat gtaccacata aagaaagttg
    12961 ggtctaaaac tatttgatgt attggcattt ggtgtggcat agagtacgct tccctcttta
    13021 tcttcaatat aaaactcaaa ggattggtta ttgtagtgaa acagccctgc cacctcttga
    13081 atatcaagcc tatcgctatt ctgaatcagt tccaccaatg gctgatagga ttttactgtt
    13141 tgctgtaatt ccatcactct gaaataagac acaaattgct gtgcaaacag tgttgctgtt
    13201 acaccgacaa gcagtagcag gacaatgatc gtataggaaa aaaccttaat gaaaatccct
    13261 tttctttcca ttattatgat tcctccaatc ggtatcctac accgcgaatg gttttgatga
    13321 tattttccgg cagcttggcc cgcaaatttt tgatatgagt gtggactgtg ctgccatcac
    13381 cctcaaagtc atatccccat atacgtgaca aaatgatttc atgcgttaag gttctgcctt
    13441 tgttctgcac cagcagcaaa aggatttcaa attcttttcg tgtcaggggc agctctgtac
    13501 cgtcacaaag taccgtgaaa tcctccggca gaagtgtcag cctgccgaca cggatttcct
    13561 ttgccagcgc accgctgcgc cgcagcaggg cttccacccg ctttagtaaa atctgcatct
    13621 tgaatggctt tgttacatag tcgtctgcct ctgcatcaaa cgcccggatt tggttttcgt
    13681 catccgacag ggctgtcatc atcagaatgg gagtatcatt tttcgcacga aattcacgca
    13741 aaagttcatg cccgttcata ccgggcagca taatatcaag aataaccagt tgataagtgt
    13801 tttcgtaaaa cttggtgtat gcctcatttc catctgtgca ggcatccacc tgatatcctg
    13861 cctcagccag aaacgccctt actgtattac agatatgatc atcatcctcg acaagtagaa
    13921 ttcgtatcga cataaaaatc cctccatttt aagaagataa cataacagtc tgtagtttcc
    13981 ctgtagtttc aaagcgattt cttttcggat tttaatcatc cgtcattggg atactcgctt
    14041 cttttcatgg tgtatgtatc tatattattg gtttaaacga attaaatcaa gataataata
    14101 attaacacct tcttaatgtt tgggatgttt cgattacagc ggttgtcaaa acatcccgat
    14161 aatactggca agcaatggat cagaatatcc tttacggata ttctgatcca ccacttgccc
    14221 gcctgcggcg ttctcgtttt gcttgttggg gacaccccaa gcctcagcaa aatgcgcggc
    14281 ctcttttttg cgaatgcacc ttgcggtatg gcttttcaca caggcagggc ctgtgtgtac
    14341 tccctccggt cgctcttgcc gcactccgcg cagcagattc agtcctaata ccgttcaaga
    14401 cagtagaaaa tcagcaagtc tatattcctc atatcttcat catccgtgtc cttgcagtta
    14461 caggaaatgt aataatcgcc aaatgtaaat cccagaatat tttcctcaaa aagccgtacc
    14521 tccccggtat taaaatcaat caccccatac tcaaaggcgc attagaccag cagttatcca
    14581 atgtctggag acttcaccat aaagaatcca gatgccggcc ttttcaattc taccatatac
    14641 ggctgataca gcagcagctt cctcgtcttt gtttcgggca ttttccattt ctgtcatgat
    14701 aaagtttcgc ttcttcatac atccatgtgt aaatcttttc tttctgtttc agatgagaat
    14761 actttttatc ggtctggagc agcctgccat ctatcatctt atagtttttc cgtgacaaaa
    14821 taccatgctt tctctctctt caaaaccata tccaaaatgc gccggtttca ttatactatt
    14881 cagtaaccgc ctttcctttc tttgtacgcc tgtcaatcgg cttttccgca gttttgggaa
    14941 tgagccagat cgtcgccatc tttacagcgc cggggatgcg tcccgcagca caatagtagt
    15001 ttatccatcg tgggggcaca ccccatttct cgcctgcctc tttcaacgtc atgtagtcca
    15061 tagtacacct ccacagggta cattataatt cttttgctcg aagaataaaa gaaacaaaat
    15121 gtgaaatata aactttaaga aaggtgcata taccaacatt ccacgggcag agtatgaaat
    15181 atacaatgta agtgggaggt gcgatatggc gaatattgaa gattgtcccg gctttgaaac
    15241 tttcggcgcg gatgtgaaag cggcccggca agcaaagcgc gtttcgcgca aagcaatggc
    15301 agagaaaata aatattgact ggcgctatct tgccaacatc gaaaacgacg ggccattccc
    15361 agtctgccgg ttgtcataca gcttattaaa gcctgcgggc ttcctgtgga acgctacttt
    15421 aacccggaga tcatgcggga ggaaagcgaa ctgcggcaaa gggtcagcca caagctgaaa
    15481 ctctgccccg aagaatacct gccgatcatc gagggcgcga ttgacagcgc catactaatt
    15541 gaaaagccga agaagaatga aacggaggat gtgtaactgg catcctctgt tttactttta
    15601 gcgggagctg cacagcgtag cttctatttt tttgcacttt tttctttcca gagggctatt
    15661 ttgcgggtag tttggggctt tggaaacgtc ttgttagtgg agaaacagta acgtagcaag
    15721 catagggagt gtagccactc ccggaaaaac ggcctgcccc gtccctgccg ggccgcgtcc
    15781 gttttgcttg ttggggaaat ccccaaaccc ctgaatggaa aggacggtga agcatggcaa
    15841 acagaaagcg aaaaatcgtg ctgcgcgtcc ctgtgacgcc ggaggaacgg gctatgattg
    15901 aacagaaaat ggcgctgctg catacgaaga acttttcagc ctacgcccgg aagatgctca
    15961 ttgacggcta tatcgtcaac atggacacca gcgacatccg ggcgcagacc gccgagatac
    16021 agaagatcgg cgtcaacgtc aatcagattg caaagaggct gaacggtatg gggccggtgt
    16081 acgcacagga catcgaggat ataaaggggg cgctggcgca gatatggcag ttacaaagat
    16141 acatcctatc aagtcaacgc tgaaaaaagc ccttgactac atcgagaacc cggacaaaac
    16201 tgacggcaag ctgctggtgg cctcctttgc ctgctcttat gaaacagcgg acattgaatt
    16261 tgaaatgctg ctggcgcagg cataccaaaa gggcaataat ctgggccacc acctgataca
    16321 atcctttgcc cccggcgaag ccacgccgga acaggcccat gagatcggga agcagcttgc
    16381 cgaggaagtg ctgcaaggga aatatcccta tgtgctgact acccatattg ataagggcca
    16441 cgtccacaat cacattattt tctgtgccgt ggatatggta aatcagcgca agtacatttc
    16501 caacaaacag agctatgcct atatccggcg caccagcgac aggctgtgca aggaaaacgg
    16561 attgtccgtg gtgaagcccg gccagagtaa gggcaagagc tatgccgagt gggacgccca
    16621 gcggaaaggg acgagctgga aagcgaagct caaagcagcc attgacgccg ctatcccgca
    16681 ggcaaaagat tttgatgatt tcctgcggct catgcaggcg cagggctatg agatcaagcc
    16741 gggaaagttt atttctttcc gcgctcccgg acaggaacgc tttacccgct gcaagacatt
    16801 gggggaagcc tacaccgagg aagccatcaa agagcgtatc aaaggccgcg ttatcaccag
    16861 agcgcccaaa gagcgcaagg ggatttcttt gcggattgat ctggaaaaca acattaaggc
    16921 ccagcagtcg gcaggctatg agaggtgggc gaagctccac aatttgaagc aggccgcaaa
    16981 gactttgaat ttcctgactg aacatgggat agacacctac ccggatttgg aaagcaaagt
    17041 ggccgagatc accgccgcca gcgacgaggc cgccgctacc ctcaaagcta tggaacaccg
    17101 gcttgcggat atggcggtgc tgataaagaa tatttctacc tacaagcagc tctggcccgt
    17161 ggctatggaa taccgcaatg cagcggataa ggcaaaattc cggcgtgagc atgaaagcac
    17221 gttgattttg tatgaagctg cggcaaaggc cctaaaggga cagggtgtga aaaagctccc
    17281 cgatctgtac gccctgaaag ccgagtacaa gcgattggcc gaggaaaagg aacggctgta
    17341 tgagaagtac ggcgaagcga aaaagcagat gcaggaatac ggcatcatca agcagaatgt
    17401 ggacggcatt ttgcgtatga cgccgggaaa ggaatggacg caagagctat gagtattcca
    17461 cgaatgaatt accctcaata tcgcaaggcc cggaagctga cgcatgagtg ctgcaactat
    17521 tgtgacggca actgcctgtt gctggacgac ggcgaagaat gtatttgcgt acagagcatt
    17581 tcctattcgc ttctgtgccg ctggtttaag gccgctgtcc tcccgctgga tgcggccctg
    17641 tatgccgaga tcaccaaaga ccgggacgaa gtgaaacgct gctgtgagtg cggggccacg
    17701 ttcacgccga agtctaaccg ggccaaatac tgcgcggcct gctccaaacg gatgcgccgc
    17761 cgcaaagagg ccgagcgcca gcggaaaaga tactggaaac ctacgcactt agagctgtaa
    17821 aagcccttat atttcaaggc ttttccggca tggtcagcgg ggtatgtggg tgttttatcc
    17881 tatccccgcc gaagtgctgt tctaaatgcg tagaatttac cagcagaaaa gcgccgtttt
    17941 ctctggttcc atgcttccga atatggtatc atagaaaaaa tcagtctgac gggggtatca
    18001 ggatggacgg acaagaaaag ctgctggact atgaaacgat caaagccgcc gttgccggtg
    18061 agaggtgggc cacggaaaaa gtgttggccc attatgctga ctacatcaac gagctttcca
    18121 cggtggagat cagacaggca aacggcaaaa agaagaaagt cattgacgaa gatatacggc
    18181 agcgcatttc cctgaaactg ctggaagcat tgccgagctt cccgctggaa cagagataga
    18241 cggcaagagg gctgcaccct cttacgccgc tttgcggcta tcacctgcac acccctgaaa
    18301 cagatacacc cgctgcgcgg ctatatctgt ttcaggggtc taattatagc tgcaacctac
    18361 gcatttaagg gattgaaaac cgcccgctgc aaggcttctc ccgcatgttt gcaggggcaa
    18421 gcgaatactt catcctatcc ccgacaatac cgggttctaa atgcgtagga ataggcaaaa
    18481 agaaaagacg ccggacgcaa gcgaccacat ggccgccgcg cccggcgtct gctgttcgtt
    18541 attcgtctgt gaagtggaac ttctggccgt catagtcggc atccgtcatg cgttccagct
    18601 tggaaagggt ctgctgggcc tgcgcccgca tatccccatc cataaccggc atgacggcgg
    18661 tgagcctgtt tatcgttctg cgccggtctg cattgtggta catacagatc aggttttctt
    18721 cctccacggt aaagtgcatc attccttata cctccaattc attctttttg gttctggccg
    18781 ccgccttttt tgggctggcg gcctgtttgc tgtcggatag ctgcttgcgg atagagggcc
    18841 ggggcctgcg aatactcaca tcccggcgct cgtcccggtt aatcacggca tacacgccat
    18901 gcctgccttt catcccggta aaacacaggg atttataggg cagcatggca aagagccggt
    18961 cattatcctt ggtggaagca atcgccgcaa agtgcgggga gatctccacc atgaaatgcg
    19021 ttttattggg gctgttaggc gcggacagat ctttcaggct gtccacaata cgccgggcct
    19081 cggtttcaat ccggctgtca tgcaaagcgg tgacgtggac tttgaaatcg ccggggacgg
    19141 cccgcccccg gctgtgctgc tccgtccgta gcgcctcctg cagcacagcc gggtcatggg
    19201 gctgggcctc atagtacaaa aagtttccca atacccggtc agggcttccg tcaaaacgct
    19261 ggctctccgg gatgtcacgg gtgccatgct cataaaagag ccggttcaca ctcaccggct
    19321 gggcggtttc aatgacgtgc cggaaatgct gcggatagtc cagctcatag agattgcccc
    19381 ggatagtgcc gccctgtgtg ccggtcagct ccaccgcgta ggccagtatc ttgtcacggg
    19441 tctgctcccc gtaaaatttc catgtgttgt actgtcgggt gtctttcagg aaaacatcac
    19501 gttcccggaa acagtacgtc ccggagggcc gggacatcca gagcagggtc ttgtctgcgg
    19561 cctgatcgct ggcggcatcc cgcctgataa tgtctttgtc aatgccaaag tcactttgat
    19621 aaaacgcggt gttctgccgc atgatctgtt ccagcgcggc cagaacgtcc acattttcaa
    19681 acttctgcat ttactctacc tccaatccct ttgatttttc ccgcgccggt ggctgcttgg
    19741 cctgttcggt cttggccgcg ttgagctgcg cccggataga gggcttttct ttctccctgc
    19801 gggccgcaag ctgtaaggcg ctgggctttt cgccggaaag gggctggatg cacttgatac
    19861 ggtcagcgac gcggattgtg gtgtcgttta actgccgctg ccatgccccg gccttgggcg
    19921 accagcggaa accgttgctt ttcagctcgt cgcgtacagc agcatccggc ttcccctcaa
    19981 agaagatttg caggcggttg gccacccggt tgatctccac agtgccgccg tcaaactccc
    20041 atcccacata ggccgcctcc ttttggcggg tcaattcctc aatgcggccc tttaaccggc
    20101 gtatctcggc gttattgttg gagagctgcc aggactggaa cggttttttc tcatagtgcc
    20161 agcccgacgc catatccgct ttcaggtttt caatgcctct ttcgatcaga tgggggcatc
    20221 cgtccagcgt cccatgcttg cggtagtagg cattgacggc cttcatcaac tcctgcgacg
    20281 cttccagctt ttccagtttc ttttccagct tggaaacggc ctgcggatcg tcggcgctga
    20341 taccacccat gccggtgctg cggattttgt caagcaagcc ctgaatgtac tgaaattcct
    20401 ccatgttgcg gtcactggcg gcgttctgct tttctttcgc ccgtaccggg aaattggacg
    20461 gcccggcaat caggacagag gggacgcggg cggtaatctc atagccctta ttcatgttct
    20521 cggccagctt gcgggcgtag gtgtcaagga ggctgtctat cttttcgtga tactctggct
    20581 ccacacgctt tttctggcgc tccgcaattt ccacggcctg atcgacatag tgccggtatt
    20641 ccgccgtggc gctgccgggc cggtagtcag aaaagctcat ggcttccttg gcgcggcggg
    20701 cggctccctc attgatggga taatagcgga cagccgtttg cgggggcgca gcctccgggg
    20761 gttcctcctg tgtctggccg gaagcaactg ccacatcctc aaaagatagc tgttcgggtt
    20821 cctccggctg cggttcgggc ggcgtattgt tgataatgcc gtcaatcatg ttttcgttct
    20881 gctccgtaga cagttcggcg gtacgcaggg gattttcccg caaaaactcc ggcacttcca
    20941 tatagccgaa gctgtctaca tagtgggcgg tgtcctgtcc attctggtgc agcaccacca
    21001 cgtcggaaac ggaaaggctg tgtcccctaa aatctgccgg gtggtcgatg ttgaatgtgc
    21061 ggtaaatatc ctccaaagac gtgcttttat ccagcggtgc cgtataaaca aggtcatagt
    21121 tggcactgtc cacggccagc cctgcggcct gtaaacgctc gtatggctca aaccgatagt
    21181 cgcgggtttc ctccccgcct cgaagctggt agatggaaaa ggtgtcctgt tggggtgctt
    21241 catagccagc cgccgcgtat tcctccacgg tcataccggc tgcggcggcc tcctgcgcga
    21301 tctcggcctg tacctgtgct ttgtagtcct gcaagctctg gtcgaagtca gccagtttcg
    21361 gcggctccgg caactgatac tggccctgat tgagcatggg gcggcactcc tgcacataag
    21421 agagaagcgc ccccaaatag gccgtttcct ccggcgtcag ggtttccccg gactgtaagc
    21481 ggccctgcgc cgtctgttcc atttgggcaa ggctgcaatg cagcttcata taggggacaa
    21541 actcgcctaa cagcatatcc cgatgggcct tgtcctgctc ccagccctct atgccggttg
    21601 tagcgattac atgctttttc cagttttcgt cctgcgcgta aaattggtgg tagctctgga
    21661 tatgttcgat cagggaaccg tccctatctc caaaatcctg ccgcccctcg tagctgtccg
    21721 gctcgccctg aaagatacag tcaatgcgga acgcggtttt gtcatagcct gcctctgccg
    21781 gatatgctgc atccagacgg gcaaacagcg cgtcggcctc atgcaacggc atttgctggc
    21841 cgtcctttag ctgggggctt tcgctccata gcacagtcac aagcggctcg gctgccggat
    21901 caagggccgg ggcctgttct tcctcggcct gcgccggggc cgctgcctcc ggggtaacgt
    21961 caaccgtgac ggcctcctgt tcctgccggg cctcgcgctc ctgctgaagc tgggcaaaat
    22021 ggccgtcgat ctcggttatc aattcgctgg ccgtggcccg gatggtttca agggaggctt
    22081 tcagctcgga taactccctg ccgctgctcc aatcggccac atagccaaaa gaatagtccg
    22141 acgtgtccag cccgtaatac tggcagacgg tataggccac gctttcggcc tgtacctcgc
    22201 gggtgctgcg gtctggccgg gccgcctgtt cctcggcggg ggcctcccgg tcaatggcgt
    22261 ggagcttggc atgggcaatc tcatggatgg cggtctttag ggtctgcaat tcgctcatgc
    22321 cctcgttgat ggcaatgcgc tgttttgcgt agaagcagcg gcccttggcc ccgtctgaaa
    22381 tcggctcaat gcttatggga tagggtgatg tccgttccag cgcggcaaag aaatcctgat
    22441 actgctgcac gtcgccggta agttcactga tatagggcat gggaaaatcc ttgccctcgg
    22501 tctggctgat gtcaaagact gtcaccacct taaaggccgg aaccgtcact tcctgttctt
    22561 cggtcacggg cttgccgtcc gcgccgatca cgggctgctg ggtggccggg tcggttttct
    22621 ccatctgctg cttaaccttg aacggggccg gggccagtat cttaatgcct ttctcgcccc
    22681 gcttcacatg gcggtcaaat tcatcctgcc acctgttata gcccgccaca atcgaaccgc
    22741 cctgcatggc gatcagcagc gtattgttca gactgtaatc gtgcatcttg gacatagcgg
    22801 ataagtaagc catatagcgg ctactttcaa aaagctcctg tacgccggtt tccagacggt
    22861 cagtaatcac tttcatgcgt tctttgctgt tcatgccgtc cagcatgatc gggataaccg
    22921 ggggcggcgc tttgtcgggg ctttcggatg cctccggctg ctgggcctgc ggttcctccc
    22981 ggtcagcttc cggctggggg tagtgcatca cccggtattc ctccggcacc ggctggccct
    23041 catggacgcg ctgccattcg tcgccgcttc tcaccagata gccgtactgg gtaaaaacgc
    23101 cttgttcctg ctgggcaatg tggcgtccaa actgcgccgt atcaatcccg gctttccatt
    23161 cctccggcat atccaccatg ccggaacggt tcaggtagta gtcgcccaac tgtccatccc
    23221 ccctaacgtc gggaagatgc acatagtaat ccacattttc ggtgtagtcg atgatctggc
    23281 cgatattctc aaagccgcca cggggatttt gcagggcggc gttcagctct gcgatctcgg
    23341 cggcgtcaag ctggcccagc cgggcggcaa ggaaattcag ctcgtccacg tcggcggcca
    23401 gcaccatgtc atagggaatg gcaaggtgtc tgtcctccgg gcaggaatac ccgttgataa
    23461 aaaaatcctg cggattgtcg gcagtaatgc caacatcccg cagggcggct tgcagcgttt
    23521 cggttgtggt gggcaaatcc agccagacgc cttgtttgcc ctgttccaaa aactgccggt
    23581 tatggacttg gatagaaaat acttcgttca cgcggtcact cctttctctg ttctgtttca
    23641 aaaaataggg ggttggggac ttccccaaca agcaaaaatc cccggcccgg cagggacgcg
    23701 gccgggggat tttaccggga gtgtagccac actccctatg cttgctgtgt tactgtcctt
    23761 tagctcctgt gttcaatgcg gtagttgacg tacttgcccc cggtatcagc caaaagcacc
    23821 gtcccgtcgt aggttttgcc ggtctttacg gaatagaggc cctttacctt gactttgccg
    23881 gttttgagca gcgcagcggc gatcttcggg gtgaacgctt tcttgcgttc ctcaaaaaat
    23941 cggtcattct tccacatgac gaactggcag gcccggtttc cgcagtagta gtttttcttg
    24001 ccctcataga cagcctcacc acagcgggga cacgtcccga taggaacacg ctcggcctga
    24061 aacaatttct gcttttcctc actgatacag gaataggtct gcaccagctc ccgcgcctgc
    24121 gtctcgatgc cgtccataaa ttcgtcagga tcggcctgcc ccttggcgat ctcggtcagc
    24181 cggttttccc attcggcagt aagctgggga gaagtaagcg cctccggcag caccacagcc
    24241 agattgatgc cgtccttggt gggaatgagc tgcttgcctt ttctctggac aaagcccatc
    24301 tgcaccagct tttccagaat ggcggcgcgg gtggccggtg tgcctaatcc cttgcgctcg
    24361 gcatcctccg gcatatcctc ggccccggcc cgttccatag cggacaaaag cgtatcttcg
    24421 gtgtagggct tgggcggcgt ggtgaaatgc tcggtgacag aggcggccac gtctgcaaag
    24481 gtctggccct cgcaaagctc cggcagctcc cgcaccgcct cggcgtcctc gtcggcgtcg
    24541 gtctttaagg aagaacggaa ccggcgttca atctctttcc agccggggga aaggacggtc
    24601 ttgcccttgg cggtaaactc ctgcccggca caggtaaatg tggcggtgac ggcctcatag
    24661 atatgcggcc cggccacggc acataagagc ttgcagcaga tcagggacag cagctttttt
    24721 tcactctcgg ccagagtgcc aaagccctgc ttcacaaatt cagcggtggg gataatggcg
    24781 tggtggtcgg acaccttttt gctgttcagg atgcggtcaa actgcgggga aaggttcagg
    24841 cccttggcaa acggcaccag ctcccacagg ccggacacaa ggctttccac ggcgggcagc
    24901 atatcgtcgg taagatattg actatccgtt ctcggatagg tcagcagctt ctttagatac
    24961 agtgcctggg catagtcaag ggtctgcttg gcggtatagc cgaaaagccg gttagcctcc
    25021 cgctgcaagg tggtaaggtc atagagcttg ggcggctgtt ccgtcttttt ctcccgcgtc
    25081 acggaaacgc aaacggcctg cgcttttgca caagccgcct tgacggtgtt tgcctcggca
    25141 gcgtcggaaa agcggctgct taccgcgtcc gcgccgcccg ccgtgatatg gacgatgtga
    25201 tatttctctt tcttgaaatc ggtaatcttg gcgtccctgt ccaccagcat ttttaaggtc
    25261 ggcgtctgga cgcggcccac agtcaggttc ttgtggtaca ggacggaaaa aagccgggtg
    25321 gcgttaatgc cgatcagcca gtcggccttg gcccggcaca gggccgattg atacagcgga
    25381 tcatagtcgc cgcccggttt caggtgggca aagccgtcct taatagcact gtcctccata
    25441 gaggaaatcc aaaggcgctc aaagggcttt tggcatcccg ccatctggta gacgaaacgg
    25501 aaaatcaatt ccccctcgcg cccggcgtcg gtggcgcaca caaggccggt cacgtcgggg
    25561 cgctgcatca gcgaacagag aacgtcaaac tgctgtttct tgtcgtcggg gacgatgtac
    25621 tgccatgttt ccgggagaat gggcaaatcc tcatagcgcc attttttgaa acgctcgtca
    25681 tagcgtccgg cgtcggcaaa ggacaccaga tgtccgatgc accagctcac cagatagccg
    25741 ttcccctcca aatagccgtc gtgtttctcc gtggccccga tcacgcccgc aatgctgcgg
    25801 gccacgctgg gtttttccgc gattacaagt ttatatgtca ttcctcgtcc tcctgttcac
    25861 tcacaaaatc agcttccggg tcaaactcgg tttcgggttc ctcgtcctcc aaatcctcgt
    25921 cctccccaaa atcatactcg cccaaatcgt cgctgccggt ggtttcagcc ttgggctttt
    25981 tgaacttgaa atagtaaagg gccgcaccgc cgcccgccag cgccaccagc aggaacacga
    26041 tcaggccgcc cgcgccgccc ttgggttctt cctccggggc ttcgggttcc tccggctggg
    26101 gttcctgcgg ctccgggccg ctgcactcgg tcaggttggt tttacatacc gggcaattcg
    26161 tgttcacagc cccggccttg catttcgtgg tacaggagca gacagcagcg ggggtgacgg
    26221 tttccccgtc ctccatcagc gtcagcagat cggcttcatc cacctgattt aagaaatgga
    26281 cagtttcttt tccctcgtcg tcccggtcaa taatcagata aaacacattg ccgtttttgg
    26341 tttccacggt gataaactgc ttgccatttt tcgcggcgct gccgatgtcg tcaatcagtg
    26401 acaggttccc gtccggggac agggcggcgc tggccccgcc gccattgaac agttccagca
    26461 gagaagcaat ttcctccggg tcaatggaga gggaaaaacc gccctcgccg gtagccgcta
    26521 cttccttttc gctgacggtc atatagtcgg aatggacata gccgatgcgc tccggcaaga
    26581 gaattttaat ccagtcgccg tctgtgccga ctacctcaac cgtcgtccca ttgggaagct
    26641 gggtgaacgc ggtcttgtca agccccgcgc cggtgcggac attcagcctg ccgccgttgg
    26701 tggtgacggt gccggtctgt tcaggttctt ttttgtcggt gccggtggtg atctggatgc
    26761 cgtcgtccgt ggcggcaatg ctcaccttgg aaccgaacag cgcggaaaac aggtcggtga
    26821 tgtccttgcc ggtcagaggg ccggtatctt tttcggcctc ggtttctgtc ccgtccgtgg
    26881 cggtgccggt ttcgtctgtt tcaggctcca aaccgcccgt agcgggttcg gttgtagcct
    26941 ccggcagcgg ggccggtgcc gggtcattgc cgcctgcaag ggcggtaacg ggaaaggccg
    27001 tgacgcagag catcacggcc agaagtgcgg ggagaatacg ggaaatccgt ctggtcttat
    27061 tcttcatggg cattgtcctc ctgttcatgg taaggggttg cggcaggggc ggcctgcatt
    27121 gcgccgctgc gtaagaacgc ggcaaattcc tccggcttca tgttcatgcc gcgcacaagc
    27181 tggatgatct gcaaattctc ctgttcggtt ttctgcgttt ccagctcccg aagctgtttc
    27241 tgaaactccg cgatcttaga cttggttttc tggatgtcct tttcgatgcg gtcaagtttg
    27301 gttgccataa ttgaaagctc ctttctggcg ttaaggtaaa cgcccgtaac tgtaaaaatg
    27361 ggattgccag tagtttgaag taaggttcgt gtaggaaatc gggttcccgc aatgcagcat
    27421 cacattgttg cccacataca ggccgacgtg ggacacgccg ggggtgtcgt aggttccctt
    27481 gaaaaataca aggtcgccgg gcttggcctg cgaactggaa acaggggtgc agatgttata
    27541 taggccctgc gcccccagcc gtcccacgtt ccatccacta tgattgatta cccacgaaat
    27601 gaagccgctg caatcaaagc tggtgctggg agagctgccg ccccacacat agggataacc
    27661 gatatatttt tccgcctctt tgagcatggc ggcaaaggtg gcatcctcca aagcctccgg
    27721 gggtacgtcg tagtaggtcg gcggcgtcag ggtggaggcg ttggggtact ggctgccgga
    27781 aaacagatca ggccggtttc ctagcgtgga catatacagg gaatagaaac ttagcgtttc
    27841 ctcgtccatg atatagactg gcacatggga caggtcgaag ttttccagcg tgacgtagca
    27901 gatgtaatag tcatacggca cttggtaggt gtctgtatgg gtgttgccct ccgcgtccgt
    27961 ccatgtatcg gtttcggtgc ggtaacgggt ttctaccacg acgttttctg tcaggatata
    28021 ttgcttgtca aagagcatgg acaaatcacc ctgtacctcc ccgatggtaa atatcccgtc
    28081 atggagcgcc gacagaatgg aaatcagcac ataggggtca tggctgatct cgtcaaggtc
    28141 aaaatggtat tcgtcatagt catgggtggc ctcgtagttg tccagatagt cttgcagctc
    28201 ggcttccagc tccgcgtagg cgtcctcggc ggccaacata tcgctatcct cggaaaagta
    28261 agaggacgcc gtgatgccgc ttccggcgct gccgaacatg gcggtgcagg actgtaagcc
    28321 gccgatgata agcagcatca aaagccccaa cacaaggacg gccagcgccc ctttccagtg
    28381 gcgggccaca aaggaggccg cccgttcact ttctttggct gcggccttgg ccgctttcgt
    28441 ggaggccccg gctgtcttgg ccgcgccctg tgccgccgcg cctgcggtct ggcccgctgt
    28501 ccgggcggcc ttggcgtact ggcgcttgat ctgctgtttc tgccagaacc gggaaatggg
    28561 gttgctggcc gcctgtgcga tctccgggtt atcccgcaaa gatttctggt agaaatactc
    28621 cgcgttggca gacaatagcc tgcgctcggc cttttccgcg tcccagtagg gtttcagcct
    28681 gtggtgccgg atggtgctgc ggatggcttt tccggcctgc cgctcggcaa gttcctcccc
    28741 cttatggccg ccctccacac ccacgttttc atgctcgact tcatggattt tcccgtggcc
    28801 gaatagaaaa acctcggaaa caggacggcc tgccggattg ggtttcaggg agggaggccc
    28861 cttgtcctgt ttgtcaaagt gcaggacggt ctttgctttg ccgcttgcct cgtcaaagac
    28921 ggtttccttg accgggacgc gcttcttggg gatggcggcg cgggccttat ccagcttatc
    28981 ggcccgtttg tccgattttc ggatataggg ctgtaaatcc gggtcggccc gttcttcctc
    29041 cgacagttgg agccgggcgg atggccggtg cagggcagcg tccccctcgg cggccacagc
    29101 cccggcctgc ttcgcggctt tcttggcctt atggccgtca tggatggcgt cggcccgttc
    29161 cagcagctta tcggcggtgc cgcccggccc cggcgcggac aaatcctgtt cggcctcccg
    29221 gctggaaatg ttctccacct cgccggtggc ctgattttcc agcgtcagcc cgtcgcgggt
    29281 catggcctgc gtcactttcg ccttggcttt cagctctttc atggtggttc acctcctttg
    29341 tggcgtcgcc gggtcatact cccgccacct cgtttaagcg ggtcgtcata atcttgtaaa
    29401 gctgggtgtt gtgcgggatg gggttggtaa agggcagcac cacgttttca aagatcaaaa
    29461 ggccctcgcc gggttcggaa ttgtcgatgt acttctgctg ttcggtggac aaattcaagc
    29521 gttccgcaag gatttttcta tccccggacg cctgattgag cagcgtaatg aagtcgctgt
    29581 tttcaaggat attttcaatt tccggggagg aaagcaggtc tttgacgttc tgggtggcac
    29641 ctgttggaat gccgccccat ttcctaaacc gcttccaaat ctccgcagaa taggccgccg
    29701 tctgttcctc tttcaaaagc aaatggaact cgtcggcaaa ataccatgtg gcctttcctt
    29761 ggcttcggtt ggccgtgacg cgcccccata tctggtcttg gacaatgagc atccccagct
    29821 ttttgagctg cttgcccagc tccttaatgt caaaggacac aagccggttg ccgatgtcca
    29881 cgttggtacg gtggttaaat acgttcaggg agccggacac atacaggtca agggcctgcg
    29941 ccacccggtc agcctccggg acatgctgat ctaacaacgc tttgtggagg tcggacagga
    30001 tcggcatatt ctccgggcgg gggtctgcca gatagggccg gtagatcacg cgcacagccc
    30061 ggtctatcac ggtcttttca atggcttcca gcccgttctt gccgcccatg acaagctcac
    30121 agaacgacag cacaaagtcg gatttcaggg ccagcgggtt atcgtcctcc gaataattca
    30181 ggttgatgtc cagcgggttc acaaagtcct tgctggtagg cgacagccgg attacctgtc
    30241 cgtggagccg tttgacaagg gggtaatatt cggcctccgg gtctgaaata aaaatatcgt
    30301 caggcgtcgt taagaacaca ctgacgatct cggatttaca gctcatggat ttgccgctgc
    30361 cgggggtgcc gagcttcaag gcattggggc aacgggcctg cttcctgtcc agcataatca
    30421 tgttgcggga cttggcgttg atgccgtaat acatggccgc gccgccctga aaaagctcct
    30481 gcgtcacaaa ggggacgaac acggccacgc tggaagtcgt aaggctgcgc tgtatcttga
    30541 tctgattgac gcccagcggc aggctgcttg caaggccctg ttcctgctgg tagtccagcc
    30601 ggacaagggg acagttatgc ttttgggcca cgcctgcggc ttggaacacg tcgttattca
    30661 gcttttgctt ggtgtcggcc acgttgagga caaggaacgt caactggaac agccgttcat
    30721 tgcgggtctg caactttgtc agcagcttct tggcgtcctc gccgtaggtg gcaaggtcgc
    30781 tgggaagaat gtccatatcg taaccgctgc ggacggcctt tttctgctcc gcaatcttca
    30841 tagcgtcaag gtcggtaatt ttgcgcttga tggtcttgat cgccttggtc tgttcaatgg
    30901 cctgcacatg gagattgact aaaacaccgc tttccgtatt gaggaagtcg gccagcatat
    30961 cgtccgacag ctccggggac aggatttgca ggaaacttac cgcgccgtat ttgccgccca
    31021 tgccgaatgt ctttgcattg ccgaagcaca gggaggacgg ggcaataaaa tccttggtgg
    31081 agaggccgga cggggccagc cacttccagt caaaattgaa tatctcgccg tccgggtggt
    31141 acacgccatg cagcaggttc agccgttccc agccgtccat cacatgggct acggccccca
    31201 tgctcttgaa atagcctaac aggtcaaagc cgatgcgctt caatctggct cgcgccgctt
    31261 tcacgctttc ggcctcgatg gtgaatgtca ggtatttggt ctttaccatg ccgttgttgc
    31321 cgtttgcaag ctgcttttgc agaatgccgg tgtactcggc ccggatgtcg tcgaagtcgt
    31381 ccccctgcgg cgcgatctca aagctcttgg cgtactgcac cgggtcaact ttccgattga
    31441 ggaaagaaaa ctggatatga atggaggcgt ccacatagtt atacaggtcg cacagttttt
    31501 caaagatagc cgtctgcgtg tccggctggg caagctgata gctgatgtcc gcaaactcaa
    31561 tgcacttgga aaagacgccg ggcgataccc ggcacacgcc gtccgggtac atgacctcgt
    31621 aggggatgct ctgctgcgcc gtgtggggcc tgccgtcgcc cttatattta cggattaccg
    31681 cgtcaatttc ttttcgctgg gcgcgggtca gctttgtttt ttcggtttct cttgggattt
    31741 cctttttgga caatggcctt tacctcctgt tccatctggt attgccgcac aagggcggtg
    31801 taggtgttgt cggtctgata gggacgctcc tttggctgga tgaagcagca ctggatgatc
    31861 tgcataatca ccacttcaag gggctggccg tgacgctcgt acatggcaag caaaaagccc
    31921 ggcagcatgg caaataccat gagcagggcc gccgcgctgt tggtaagata gtcacgggtg
    31981 agaaaatata aggggacgcc gatcaccagc gccccggaaa aacagatcag ttgccgtttg
    32041 gtaagcccaa acacaacctt gcttttcaca tgggttaagt ccttggggac ggttacataa
    32101 gccatttggc acctcctttc acgtcaggat aaggtcacat tccacctcgt tcccccacac
    32161 gtcccagccg ggggttttct gtctggcaaa cagctccacg cggggcaaat cgcccgccag
    32221 ctctacgatt ttttcacggg tcacatccgg ctttttgctg tgttgttcaa tcgggctgat
    32281 aataaactga tggacagagg cagaacgacg cttcggcttg ccgtgtgttg ccagcaggca
    32341 gatttccgcg tttccccttg tccagcggcc cagcccgtaa aaccatgtaa ggcttttacg
    32401 gttctgtttc agccatacaa aagccacggt cttgaatgaa aagccccacg ctttgattaa
    32461 ccgcagagct tccggcagca tggggaatgt ggcccacaaa aacagcaggc agtctttgga
    32521 cgccagccgt tccaccggca gggcgcacag ctcgtcaatg ctcatggtgg aatagtgatt
    32581 ttccgctgcg ccctgtacgt ttttgcagtc ataccgccac ggcgggtcag cgtaaatgat
    32641 gttgtacgtt gaaataaaca agttcctcct gtgtatgctt ggattttggg atcaatgcgc
    32701 cccgaatacg gcctttgata tactgccggt cttgaacagg cagaagcaca gcagcaccgt
    32761 atagcccata cagccccaga ttgcgccgga tatgtcggaa gcggaaccga tattctgtat
    32821 cagcaccgcg tagataccca ccacaaccat gattaaaaag gcttggaagc ccagcgccag
    32881 cagggatttc agataccctt ggcccatgcc gcgccattcc gcgttgccca ttgtggcaag
    32941 ggggataggc cccagcgacg tgacaacata gatttccacc atgcggccat aggtcacaag
    33001 gaaaatgcag atggaaagga tattcatggt gaggcccaca acaagggttt ggaaccacag
    33061 ccccaaaaga gcgcccacgc tcatagcctc caattggctt tcaaggtctg gtatcagggt
    33121 gttaaaatca atggatgtct gcccgataat cacaccggcg ctttgattga cgacgctttg
    33181 ggtggcgtcg aatacgccca tgacgatgtt ccatgtgttc gttacgatca ggattgcaaa
    33241 cgcggatttg aatatccagc ggaagaacat ggagctgtca aagtcgtgca tattgttctt
    33301 ttccgttatc atctggataa gctcatagca catgacaagg gcgagtatgg ccccggctat
    33361 ggggacgatc acggtttccg acaggctgtg cagcatattg aaaataccgc tgttccatgc
    33421 ctgcggggtt cgtcccacgt cggcggcgat ttctccgact ttggcattta ccgtatcgaa
    33481 cagcccggac aggttgcccg tgataccgtc catgaggatg ccttttatcc attcttcaat
    33541 gatttcacta atcacgggta gcacctcctt tcccgcgccc tcccggtctg gccgggagga
    33601 cgcggaggcg attagttaag ggtttggaca gggacaaagg ccaggatcag cccagcagcc
    33661 cggacagcag gggaaccaga gtgacaccga tcagggccac gccgccgcca gcgacaagct
    33721 gcttgacacc ctgcgatttt gcccccggat tgtcctgccc gtatccctcc aacagattga
    33781 tgccgcccca tacgcacaga gcgccgccca gtccgatcac gatagtctgc aaagtagtaa
    33841 ctgcggaatt aaaaaatgcc ataaatttac ctctttctgc cggtttccgg ctcataatga
    33901 ttttttgatt ttgttgttgg aatgaaaaaa gccgctgttt attcagcggc ggcgtcctcg
    33961 tccgacaggt cgatttcata caggtcgaat acttcatcct gcgttaccac ttgctgccgt
    34021 ttccggcgcg tgatagtcaa aaatctgtcc acgtcaaagg tattcttctt gtcggcgtcg
    34081 gcaaggtatt tgtaccgggg atgcttggta atatcgaatt tctccgataa aaacgggcgc
    34141 acaccccgta gctgcaagat acacttgccg ccgtccatca cggcaatttc gtcttgggac
    34201 atcaactcct ttcctaattt ttgatagttc aatccgtggg acgtttggct tccccggttc
    34261 tcgctggtgt tgaaactgtc aatcgtttct ttccccagcg catcggaaat ctccttggca
    34321 tttttgccgc gcccactaag gaaaagcaca ctatcgcagt tatccgaaat gatctcggcg
    34381 gcgtccttgt aaatcgcttt tagctgcgac tggctctgca aaataataga ggccgatatt
    34441 tcacggcttc gaatggtggc aatgagttta tcgaaattgg gtatttggcc aatgttggca
    34501 aattcatcca aaatcaggcg tacatggaca ggcagccgcc cgccgtacac atcatcagcc
    34561 cggtcacaaa gcaggttgat tagctggctt tggagcatag ccagaatgaa attaaaggtc
    34621 gtgtccgtat cgctcataat gagatacagc accgtctttt cgtccccgat ggtgtccagc
    34681 tccatttcgt cgtcctccat cagctcgcgt acctcccgga tgtcaaaggg cgcaagtctg
    34741 gcaccgcatg aaatcaggat ggaagaacgg gtcttgcctg cggacagcag gaatttcttg
    34801 tactggcgca ccgcaaaatg gtcggggtct ttctgctcca aacgctcaaa catgagatcg
    34861 acggggcttt gaaactccgg gtcgtcctcg cgggcttcac tggcgtttat catttccagc
    34921 agcgttgaga agttcatttc ctccgctggg gcctcgtacc agatatagcc gatcagggcc
    34981 gaataaaaca accgttccga cttcacccaa aaatcctccg ctgacttttc cccctcgccc
    35041 ttggtgttgg aaatcagggt atttaccagc tttagcacgt ccttttccga atggatgtag
    35101 acaaaggggt tgtacttcat ggacttggaa aagttaatgg tattcaggat tttaatgcgg
    35161 tagcccgcct gctggaacat cctgccggtt tccagaacca aagtgccttt cggatctgtc
    35221 accacaaacg aagtcggata gtccgtggtc ggaaagcatt gcatcaggtt gggtttcacg
    35281 aaaaatctcg ttttccctga accgctgcca ccgatcacca gcacgttttt attccgcgct
    35341 gtcttggggt ctttgggcct gccggtcatg gtaagccgtt ccgtttgggt gagcaggata
    35401 ttattttcaa acttggggtc gatgtaaggc gctatgtctt tggccgtccc ccagcgggcg
    35461 ctgccatact ccatgccttt gcggtatttc ttcacgttct tgctcttgca gtacaccacc
    35521 agccgcacga tcacggcacc ggcgataccg atacacaggt caaacaggtg caggcttggc
    35581 agagggttgg caaaagccgc cgcaaaacca tccgcaaaat gcaggagctt ccccgacagg
    35641 tcagcaccag cggccagccg gtacgcctcc ccgcatttcg caaacaggta cacaaaaatc
    35701 agatacggga ggttcaggat cagcaatttc tttacgtcag gcttcacagt tccagccccc
    35761 tttccttgat tttctccttg gcccgctgct tggtctgggc cgccgccttt tgcttcacag
    35821 cggacaaaac cttgcggata gagggccgct gctccttgga aagcgtcttg gcggtaaact
    35881 cccgaaacgc cagatcaagg ttatctgcat ccttggcctt gaaaatcaca acgtagtggg
    35941 gcggctgggt ggtcttatct tttcgtaagg taaaatccac gccgtatttc ttggcgcatg
    36001 gcttgaacgc tccaatgttg gcctcggtga tctcaatgtt ggagagatca gccccatgct
    36061 ttttaagctg ctttaggctc tgctggccgt ggtgcagcgt gtttttgccc ttggcccggt
    36121 gttgcaggta ctttttcagc gccgcttgca gcacttgagc cgtgagcttg cccgttttca
    36181 tggacagggc aatggtcttt tgagtaactt cttcctgcaa tcgcatccct ccttgtcaga
    36241 aagtcaaaag cggcgtctaa ggcggaacca ccagacgccg caggtagata taggtcaaat
    36301 ccgcacccgc aggccggaaa ggtcgtcggc ccggatgccg acaagatacc agttttcggc
    36361 catttcaatg cgggtgggcc tccatttgcc gccgatcatc acgtcgaaac actccccgca
    36421 atgcaggccg ccgtaatagt cggccagatc aaagcggatg tcgtaacggt ctgttcgctc
    36481 gtcaaaaatc aatgcgccct gcttcatagg gtcgtcctcc ttttctcgtt acaggctttc
    36541 gccctcgccg caggaatagt ccggcaggcc ggatttaggc tttccccaat cgggctgtgc
    36601 catgtcgtgc gcgacaaggg ccgtgtagta gttggaaatg gtgctggggg cgttgaacag
    36661 catagcccgc aggtattgtt tgatattgcg gactttcgtt gtgttctccc gcagacagtc
    36721 aaggacaaag cggatatgtt cgccggtcag cttcatgtac ttggatttca ccagctcggc
    36781 agggtagtcg tccccggcga cacggattgt ctttcgggcg gtgcagacgg tttccagcat
    36841 caggtctacg atttcgtcaa gctgttcacg gtcaatgctc ctgtcctgaa tgagataggc
    36901 atactcgatg ttctccaaaa tgatttcccg ataaatccgt acagcctcat cctttcctgt
    36961 ccttttccgt tccggcggcg tagccgctgc ctcgccctcc ataggggcag gggtcgggga
    37021 tgggatagga atggaatggg tactttgtcc atctgtaatt attttttctt tttttgataa
    37081 gtcagttctt tgtacttctt tatttaattg cgttggattt tccgacgccg gattatccga
    37141 tgtcggattt tccaacaccg ggttatccga tgttggattt tccaataccg ggttttccaa
    37201 taccggctgt tcgtagatgg tatagaccat atcggccatt ttgccttttg cgtcacggcc
    37261 ctgctcacgc cggatatatc cagctttttc aagctcccat accgctgtcc tgatcgcgtc
    37321 gatgctttcc cggttgatat aggacagtcc ttttagggta tagtcccaat cctccggcaa
    37381 ggacaacatc tgcgacagca ggcccttggc ctttaaggtc aggttcttat tccgcaggtg
    37441 gtgatttgac atcaccgtat agcctttgtt cttttccaca cggaaaactg ccatagcgtt
    37501 gttcgctcct ttcactgtga atttgaccgc tgcatagtcg gtcagcttgc ccggctgata
    37561 gggacactct gcaaactggc agaattgata tttccaatcc gggcggtaaa atcggcaggt
    37621 gccgcaatcc tccggcgcgt ccgccccgcc gccgtcgtag tgatcgaaac cgggcttttc
    37681 ctgcatcagc cgttcaaacg cccggtcagg gccggaagta aatagcatgg cgtcgcctcc
    37741 tttctctatt tgggcataaa aaagcggcgt cctgttctcc catgacggga aagaggaacg
    37801 ccgcgtttac ggtgtcgtat tcaatttatg atgtgccgta tttggggtgg cgggtattag
    37861 cagtatggtt cagcatatat gcggctcaat ccgaaagtag taaattggta gtaaattgag
    37921 ttctcatatc ctgcgaaatg cccgtatttc cggggctttc agcgataacc taaaattttt
    37981 tctataatat ttttctcatt atacaataat gaggtctttg gttttctctc aaaacccatc
    38041 ttatatctga taaaattctt tctaaattag ggtctgtaaa actaaatcct aaaaagagaa
    38101 aagtttttgt taacaaatct ccttttaaag tttctttaaa taacgcgtac gaatcatcat
    38161 atttttcata atcatctctt gttattactg tcgaactgac atcatctata tcaccatgca
    38221 ttttataaac aattgtatcc acatttggat tagttgttgc taactgagta taatttttct
    38281 taacctgagg gtttttataa gcttctcgga ttgatttttc tattagatga tcataattag
    38341 ttgtccaata agtttcaata ggtaaacttg ctagtattcg atgattttca gatatttcta
    38401 attcttcacc aaaattatcc ataagtatct gattcaaaat tcctcttgat ccaccatttt
    38461 catttttata atattgagcc agagtaacta agtctgtttc ttttttagaa tccaacccga
    38521 tttctgttgc aggatcatta agtagttccg gccaacttac atatccttgt gacatcgata
    38581 atcctgcacc tgcaaatatt gctgcatttc ctttttcaat agcttcagag aacctcttta
    38641 ttaaaacttt cttatccatt ctacccggtt cctttcgatt cagtaaattg attagagatt
    38701 agtgaaaatg gtgtttttat ataaaatatc taatgtatta cttcctttag tgcattccac
    38761 agcatctaaa gcccattttt tcaaatcagc ttttcgtaaa taagttaatt cacttaaatt
    38821 taaagaatat tttatttgca gattttcttg cgaagaatag tgctttataa tgcttctaac
    38881 atcctcggtg ctcatttgag gattactttc caatcgtgga ttcttttttt ccactattga
    38941 agctgtcgtc agttcgctat atagccatgg cgaatacgta aatttctgtt cattagtgat
    39001 ttcattgctc aaactaattg aattaggtgt attaagaaaa aacagacatt ctgtactatc
    39061 aatcatatta tttaaagctg tagaaagcat catatgaaca tgactagttg ttacatttct
    39121 tttatcataa tcatatgttt ttgtttgttc cttatagcaa tatttttggt ctattttttt
    39181 taataaatca gaactgtatc cccaaaccga agaatcaata aatgcagtaa gaccaaaatt
    39241 ttcaaatagc catgatgcaa aacgtatagc taatttttca tcagcatgag agtgagatat
    39301 aaaaatatct gcttttatag ttggaaacca atctttaata atttcgtctg catctagaat
    39361 tttgctgtcc ttagaataat aatcaactaa atcattttca ctttcaaata gattttctat
    39421 tctcttgtta atagattttt ggttgtgatc tatttgattt actacatgtg tatatgattg
    39481 cgagcttgag gagaatgcat ttaagtcagc atttgtaaaa tccaattcat atgccttata
    39541 caaggatatc cctccattct tgtattttga tccacaattt attaggcaac ataacagtat
    39601 accctatcgt tacatatccc attcattatt ataccagtat ttcttcgtga cacataaggg
    39661 tgtaacataa taatttcaaa cgaaatgact acaagtagtc attgaagcaa atcttcataa
    39721 gacacctgta gaatatcttt gatggctttc aattgatcca acttaatgtg ttggcggcca
    39781 ccttcaatct taaccaacgt ttccctagtt atagcaattt tacgaagctg taggtctcta
    39841 atcagctcgg tttgaccaat tcctttttct aatcggactc gtctaatatt gctaccgatt
    39901 acattttctt tatcaataat tttttcctct ttcaagaata gcacaccttt tggactaaaa
    39961 ttagtccttt ttcttattaa tcttaaagat tcggtatgct acaatgggac tagtattagt
    40021 ccatattcaa ttttaaggag gaattttttt gaagaaattt gcaatcattc taacttgcgc
    40081 tttactcatc ggaactataa gcgggtgtac cacttctgac acaagttcta caagtacgga
    40141 aactagtaca acaacctcag aaaagaatta cccaatacat ttttttccgg ttccagatgg
    40201 atttgaagct tattatcatg ataaaaaact agatgttcct ttatcagaaa ctgaatctac
    40261 tgaaaagatt ttgccaattg aaattgaaag aaatcaagac gatataaaac taaaagctga
    40321 tcttccctta ctgaacagca cacaaattga tagacatgaa aatattaaag ttaatattga
    40381 aaaaggagcg ttagcacaac tttatactga aaaagttgaa caattttttg aggctggtgc
    40441 caataaagac ttttcaaaaa taagcgatta ttcggataac ttttttaatg agacccaaga
    40501 tgaaatcacg aactcaactt ttatggctga agatatgtac aatatgaagt tgaaaaaaat
    40561 aaagtcgtat gattatccat taaatctaac attaaataaa gggatactct cttttgtatt
    40621 agcaggttac gttgactatg aatatacagc agattatttg gagacgcccc ttcaacacga
    40681 taataacgat attaatttta gatttatcta caattcaaaa agtaaaacat ggttagttga
    40741 tcaattgagc actataaata cgggatttca tgaattacct aagcctgaca atgaaactgc
    40801 tttggtcatt aaaacattct aaaaaagaaa cgttgaagga ataattattc tttcaacgtt
    40861 tcttttattg tttttcagtt tacggtctta cctgagcgaa taatctgatt tctaaatgat
    40921 tggaaaactt cttgtggttg ataataatct gtatctattt caaatgtttc tgagaataat
    40981 tcattgaatt tttctactga aatgatgatt ttaccattct cctctcgact gttttctatt
    41041 atttctgcct ctaattcaaa taaatctagt gacgaactaa aatcataaat cctgtcatat
    41101 tgaacaaaaa aatcaagaaa aagttgctca aactcttcct tatttatcac tagtgtttca
    41161 agcggaacaa ctttattttc atcagccatt cttccaattt cctcactttc attttcaatt
    41221 gtccattgtt gcggacgtat cagattttct ctatcatttt tcagtgtata gatcacttgt
    41281 aatacacaac aatgctttgt aaacttattt ttttagttag tcgaatcttt ttaaatgtgc
    41341 gtttcatacc atgcgcttga tatattttct cctatttttt aaatcgttta tcacaacttt
    41401 tatcgttttt tcttctgaaa aaaatcttgt ttacatcgtt acatagtttc caaaaagata
    41461 cattactagt agaaacaaca agattaattt tacttcccac tgaaatggga agtatatttt
    41521 aaatattttt tctccttcaa cttacactat aaagtatctt aatcacacga tatgctagcc
    41581 actaaattca tcatatttga ttcctttttt aagataatgt agcatctttt tatccaataa
    41641 cgcaattgtt tttaaggata aatttcttaa gatggtacat ttcaaaatgg catttgacat
    41701 atgaaacaaa aaagaaagaa aagagcataa gggaaaactg atagcacctt cctacaggaa
    41761 ggtggctaag ttggctgtgc caactggttt tctttcaaaa tcatttcata atttttgata
    41821 tcataaaaaa atccattttg gacctatttt cggtcataat ttagtaccta cttttggtca
    41881 tagtttcgtc cgtagttttg gtcatagtga ctaaggcttt tctgttcaca aaattggttt
    41941 aaatttgctt tctttaacac ttcatttata tcatagcaat tataatgttt acacaacatc
    42001 attcctaatt ttaacggttg ttcctcttta tattttttaa gttctacatt tttttgcgtt
    42061 taattacttt tctcttttct ttatattccg ctccaacttt ctctttaata gtgataatag
    42121 aattacatat ttttgttaaa ct
//
LOCUS       DASJTC010000011        41705 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.011, whole genome shotgun sequence.
ACCESSION   DASJTC010000011 DASJTC010000000
VERSION     DASJTC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.011"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(13..618)
                     /gene="dhaL"
                     /locus_tag="U4B19_000565"
     CDS             complement(13..618)
                     /gene="dhaL"
                     /locus_tag="U4B19_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEN2076328.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(646..1656)
                     /gene="dhaK"
                     /locus_tag="U4B19_000566"
     CDS             complement(646..1656)
                     /gene="dhaK"
                     /locus_tag="U4B19_000566"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEN2076329.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1631..2077)
                     /gene="dhaM"
                     /locus_tag="U4B19_000567"
     CDS             complement(1631..2077)
                     /gene="dhaM"
                     /locus_tag="U4B19_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEN2076330.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2180..2500
                     /locus_tag="U4B19_000568"
     CDS             2180..2500
                     /locus_tag="U4B19_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEN2076331.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2612..3481)
                     /locus_tag="U4B19_000569"
     CDS             complement(2612..3481)
                     /locus_tag="U4B19_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2076332.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3577..4881)
                     /locus_tag="U4B19_000570"
     CDS             complement(3577..4881)
                     /locus_tag="U4B19_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2076333.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4878..5387)
                     /locus_tag="U4B19_000571"
     CDS             complement(4878..5387)
                     /locus_tag="U4B19_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076334.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5562..7697)
                     /locus_tag="U4B19_000572"
     CDS             complement(5562..7697)
                     /locus_tag="U4B19_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2076335.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7780..9435)
                     /locus_tag="U4B19_000573"
     CDS             complement(7780..9435)
                     /locus_tag="U4B19_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2076336.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9437..9751)
                     /locus_tag="U4B19_000574"
     CDS             complement(9437..9751)
                     /locus_tag="U4B19_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2076337.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9921..10181
                     /locus_tag="U4B19_000575"
     CDS             9921..10181
                     /locus_tag="U4B19_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEN2076338.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10195..10509
                     /locus_tag="U4B19_000576"
     CDS             10195..10509
                     /locus_tag="U4B19_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEN2076339.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11239..11793)
                     /locus_tag="U4B19_000577"
     CDS             complement(11239..11793)
                     /locus_tag="U4B19_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEN2076340.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11919..13848)
                     /locus_tag="U4B19_000578"
                     /pseudo
     CDS             complement(11919..13848)
                     /locus_tag="U4B19_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14154..14870)
                     /locus_tag="U4B19_000579"
     CDS             complement(14154..14870)
                     /locus_tag="U4B19_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN2076341.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14871..16694)
                     /gene="glpO"
                     /locus_tag="U4B19_000580"
     CDS             complement(14871..16694)
                     /gene="glpO"
                     /locus_tag="U4B19_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEN2076342.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16708..18204)
                     /gene="glpK"
                     /locus_tag="U4B19_000581"
     CDS             complement(16708..18204)
                     /gene="glpK"
                     /locus_tag="U4B19_000581"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEN2076343.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18340..19800)
                     /locus_tag="U4B19_000582"
     CDS             complement(18340..19800)
                     /locus_tag="U4B19_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2076344.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19790..21085)
                     /locus_tag="U4B19_000583"
     CDS             complement(19790..21085)
                     /locus_tag="U4B19_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2076345.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21417..22094)
                     /locus_tag="U4B19_000584"
     CDS             complement(21417..22094)
                     /locus_tag="U4B19_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEN2076346.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22124..23215)
                     /locus_tag="U4B19_000585"
     CDS             complement(22124..23215)
                     /locus_tag="U4B19_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEN2076347.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23316..23750)
                     /locus_tag="U4B19_000586"
     CDS             complement(23316..23750)
                     /locus_tag="U4B19_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2076348.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24334..25791)
                     /gene="ascB"
                     /locus_tag="U4B19_000587"
     CDS             complement(24334..25791)
                     /gene="ascB"
                     /locus_tag="U4B19_000587"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2076349.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25788..27683)
                     /locus_tag="U4B19_000588"
     CDS             complement(25788..27683)
                     /locus_tag="U4B19_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2076350.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27741..29516)
                     /locus_tag="U4B19_000589"
     CDS             complement(27741..29516)
                     /locus_tag="U4B19_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN2076351.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNYWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29566..31062)
                     /locus_tag="U4B19_000590"
     CDS             complement(29566..31062)
                     /locus_tag="U4B19_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2076352.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31256..32878)
                     /locus_tag="U4B19_000591"
     CDS             complement(31256..32878)
                     /locus_tag="U4B19_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN2076353.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33206..34519)
                     /locus_tag="U4B19_000592"
     CDS             complement(33206..34519)
                     /locus_tag="U4B19_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN2076354.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34764..34988)
                     /locus_tag="U4B19_000593"
     CDS             complement(34764..34988)
                     /locus_tag="U4B19_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076355.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35008..35430)
                     /locus_tag="U4B19_000594"
     CDS             complement(35008..35430)
                     /locus_tag="U4B19_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN2076356.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35572..36282)
                     /locus_tag="U4B19_000595"
     CDS             complement(35572..36282)
                     /locus_tag="U4B19_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076357.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36300..36797)
                     /locus_tag="U4B19_000596"
     CDS             complement(36300..36797)
                     /locus_tag="U4B19_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2076358.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36834..37286)
                     /locus_tag="U4B19_000597"
     CDS             complement(36834..37286)
                     /locus_tag="U4B19_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2076359.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37421..38026)
                     /locus_tag="U4B19_000598"
     CDS             complement(37421..38026)
                     /locus_tag="U4B19_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2076360.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38476..39096)
                     /locus_tag="U4B19_000599"
     CDS             complement(38476..39096)
                     /locus_tag="U4B19_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2076361.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39098..39967)
                     /locus_tag="U4B19_000600"
     CDS             complement(39098..39967)
                     /locus_tag="U4B19_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEN2076362.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39990..40397)
                     /locus_tag="U4B19_000601"
     CDS             complement(39990..40397)
                     /locus_tag="U4B19_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEN2076363.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40416..41705)
                     /locus_tag="U4B19_000602"
     CDS             complement(40416..41705)
                     /locus_tag="U4B19_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2076364.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        11760 a         8983 c         7021 g        13941 t
ORIGIN      
        1 gttttattga acttaaggtt gattgatcac atctaaaagc gttgaaaata aaatggcact
       61 agaagcagct cctgggtcga tatgtccgat tgcacgctct cctagatagg aagctcgtcc
      121 ttttttagct accaagactt tggtgtgttc ttttgcctgg tcaatgattt cttttgttaa
      181 attccctgcc ttcaatgcct ctgtgactgg cagccaaaca tctaacatcg ttttatcgtc
      241 agcttctgcc tttcctcggc tttgaatacc cgctgttccc tgctcgatca cttcgcctaa
      301 tttttcttga gaatcaatag aatcgacctc ttttgtcgct ttcatcatgt tcatgaatgc
      361 agatccgtat aacggaccag aagccccacc gactttcgat atcatcgcca tagagaatac
      421 tttgaatgta tcgctgattg tttcaggatg atcggcttga aaagcttctt cataagctga
      481 aacccctctt cccatgttat ttccatggtc tccatcgcca atcggggtat ctagatcact
      541 gagatattgt ttgttctgat tgattgcctc tgcaaattgc gtgagccatt cttggatttc
      601 ttgaacggtt agtttcatat tatcaccctt ttatcttgag attttttacc atgaaatcgt
      661 ctgtacattg ctttctaaag aatctttcca ttgtgtctcc gtcaagtcga tcagtgttag
      721 agaaagacct tgcatatcta gtgaggtcat aaaattaccg acttttcgga aaactacatt
      781 gacgcctctt tcttccaaca gagctaaaac atcattcatg aacacgaact gttccatcaa
      841 aggcgtcccg cccattccgt tgactagtac acctgcttct tttggtaaat tcgagtaatc
      901 atctaaaatc ttagtaacaa gttcctttgc cagtacttta gaaggctgca tcttttctcg
      961 acgatagcct ggctcaccgt ggataccaac accatactcg atctcatctt ctcctagttc
     1021 gaatcctggt ttccctactt ctggtacagt tgctgctcga agcgctagac cgatcgtctt
     1081 agttgcctgt acgacctttt ctcccagttc tttcaactca gctagggaag caccgtttct
     1141 tgctgcatcg cccacgattt tgtgtacaag gacagtacct gcaactccgc gttttccagc
     1201 agtatacgta ctattttcaa cggcgatatc atcatctacg atcacacttt ctacttcaat
     1261 atcgtccata gcagctaatt cctttgccat atcgaagttc aatgcatccc ctgtataatt
     1321 cttaacgatc agcaaaactc cttttccact atcggcttcc ttaatagccg tctgaatctg
     1381 atctggagtt ggagaagtaa acacatcacc aagcaccgct gcacttaaca tcccttcccc
     1441 tacaaacccg gcatgggatg gttcatgtcc gcttcctcca ccagaaacca aaccaacttg
     1501 ttccgctttc tgattcttgg ctacaacacg ggaagattct acacgatgaa caagttctgg
     1561 atagcttttt actaatcctt ccagcatttc ttcgacaata ttgcttggat cattcatgat
     1621 ttttttcaat ttattcccat cctttctttc cggcattttg tgcttctgtt aagatttgct
     1681 gagtatcatc tgttataccg gcagtgatgg ctgctgcaaa agctccttcc actaaaggcg
     1741 catccaccag atgataacgt tgctgctgct cttcttccaa catgtctttt gccagttccg
     1801 agttcaatac cgcgctaccg atatccgcaa aaacaaaata actagctgca tcgcttgatt
     1861 cattgactgc ttctacgatt ttcatcgggt ctgatccgat ctcgccattt attccgccga
     1921 gagaaaaaat ttgcacctct tcggattttt gcatctgctc aatcatttct ttaataccat
     1981 ctgttacttt ttgactatga gaaataagca aaatactttt tttcacaaga atcttccttt
     2041 cttttacata gttgctaacg attggtttcg ctttcattaa tgaacaattc aagtatataa
     2101 taaatttcta attttgggaa aatatgtgta gtgcttcttg aagtttgtta tactttaatt
     2161 aaaagagagg agtgatcgaa tgattacaac aacaacgaac agtgtagagg gaaaagccgt
     2221 agtagcttat aaagggatcg tttttggtga ggtcattact gggatccatg catttaaaga
     2281 cttggaggcg gggttgcgaa atatctttgg cggccgctca aaaagctatg aaaacgaact
     2341 aatgggtgca agagaagaag cacttgccga aatggccgaa cgtgcaaaag cattaggagc
     2401 tgatgcgatc attggcgtaa aaatggacta tgaagtatta ggcagcgaca atggcatgct
     2461 gatggtcaca tgtagtggaa cagctgtctc cacgagataa gtaggggaca tctgttttaa
     2521 taactaaaga gagatgggac atgtagtggc actcaagaat tagatttttc agtctaactt
     2581 cgaggtactc taccttccca tcctctcttt tttatttctt tttgcggatg attccgtaag
     2641 ctgctgcgac taacacaccc gcccctgcta aggaataact gacagaagct gttttttcat
     2701 ttgtttttgg caaagtctga cttgttgatt tggatggctc tgtcgtttgc tgaactgttt
     2761 ctgtcgtgct tccagaggtc actgattttt ctcctgccga atgcaaaata tcttttattc
     2821 gttggaaact agctgctaag tcagcttttt ttgcttcaaa atcattagca actgtttgtg
     2881 cttcgctgat ctttccgtgg attgcttgac tggccgctgt gatatccgct tgagtttgac
     2941 ttagctttcc agaagctgtt tgagcaacct gatttaattg ctgattgaat tcttttgctt
     3001 gttggatctt cccgtcaaga tctgtttgga cagcttccgc ttttgctttc gcttcggcaa
     3061 gctttttctc aagttgtgca cggtcatttc ccactttatt tttaagatca gctaaatctt
     3121 gatcgattgc tgcttggtct tctttcaatt tgttttttgc agcttctaat gcttcttcga
     3181 gttcttttct tttattttcc aagtcttgca aatgatcgat ttggtcagaa gaggtagaac
     3241 tatttattga tgtatcttct gaagatgaaa tcgtttctcc tgaattatct gtcggtacat
     3301 tggaagtcat ttctgtttcc gaggcaccgt tttcagcacc agaagagctt atggttgttg
     3361 aaggttctgt ttgagcaggt tcagaagttt ccgcaaaagc tgtctgtccc aataagaaaa
     3421 tactggaaac agcaattggc cagattaacg gcttcattct ttttttcatc acttttccca
     3481 tccttactac tttttaacta atttcttatt ataagcaaaa atgtttggaa gaaccataaa
     3541 cacaacaggc tgtttgtcta aaaaacggtg catcttttat ttttttgtac gttccgggct
     3601 tttccatgtc ccgcttccat aacctaaaat cgttttgact gctggaatga aagtcgtcac
     3661 gatcgttatc gcgttcacta tccagaacaa aagcatatat agtggtgcga aaagaaggta
     3721 tttcaatttt cttcgtcgat catctacaat cagcgaagct agcaactgga aaaatcctgc
     3781 gaccatttca aagcagatga acacaaaact gaatgtaaaa gtaatataaa tctgttcgta
     3841 gtttccttga tatacataat gcccgattaa tccagcaaaa agaactacgg aaatacaaaa
     3901 gaatattgac cagacaatac tcaatgtttg atcaatgaag atgatcgttc ttccaatatg
     3961 tttaaaagga tggagcatca cttttttgaa attcgtcagc cagacttctg ttccaccttt
     4021 tgcccaacgt tttctgtgtc gatatagcat cttcaacgtc acaggtactt ccatgaaaaa
     4081 gataatctca ggagcaaaaa cagataacca gtcatttaac tgatgatccc aagcaatact
     4141 gatgtcttct gtcgctcgat cttgtctgaa tccgcccaca tcgatcaagg cttcttttcg
     4201 atacatcgta tttgctccac tgtacgcata aagaccaccg aaaacagctg actgtgttcg
     4261 tttaatgatg cccacgatac ttgagaattc aaccgtttga gattttgcaa tcaatttggt
     4321 acgattttgg acatccatat ttgcagtgac cgcagcaata tgtcttgcac cagggcgaat
     4381 gaaataattc acatacctaa tcagtgcatc tggctccggt accgtgtctg catcattgct
     4441 taaaatcaat ttcccttttg caaaggcaag tccgatatta aacgcgtggg cttttccttt
     4501 atttttttcg atccgaacca cacgaagatt agcgtatttt ttcatcagtc tagctaatat
     4561 ttctggtgtt tggtccgttg aaccatcgtc tgtcactaat acctcatagt tgtgataatt
     4621 caatttcgtc atcaaatact cgattgtata ttcgatcaca atttcttcat tatgggctgg
     4681 caccatgatc gtgatgaacg gttcgacact caagggaaca tctacccatt ctttttgctt
     4741 atgtttgtat aaaaacacat aacaccagac accaataaac catacaaatc cgcctaaaat
     4801 cggataagaa acgagaaaaa aatacataat agacaaacaa gtatctaaga ataaattacc
     4861 tgtatgggtg attgagatca tttcagttcc accccatttt ctttatacaa atctgcaatg
     4921 aatgtcgtat ctagattttt ctcttcggag acagaataaa aacggaccgt ttctctctct
     4981 tcttttgttc caaaacgttc cgtatacact tcttcaagta attcttgtct ttttttcaat
     5041 ttttcatctt catattcaaa agatttattt acttttttgt atagatttcg attgttccac
     5101 ctagtcaata caaatgaggt aatcaggaaa ctgaaaaaaa tcaatacaaa aaagccgatt
     5161 aagaaataca gcattttctt ttctgataaa aaagcgtaca aggaaaaatc cttggctctt
     5221 tctggtctcg ttaatgggaa aaagacccat ataaaaggaa gaacaacgat catccatcct
     5281 aaaagagcca ctaacgtttg ccaaatcttt aaccagtaat taccttttga aaaatactta
     5341 tctttcacca aaattgatgc aacttcttct cttgttactt ttttcatatc ctcacctcac
     5401 gtttttttgt tacgacgtag gagtttacca tacatttcat ttcttttcac ataaagtgtt
     5461 cctaaatttt tcctaagaaa aagaacaaaa catttctcat cagcaaatga aaaatatttt
     5521 gttcttttta tcagttgttc tttttatttt ttatctctta attatgttgc tctttccaat
     5581 acgacaatgc ttttcattcc tctagatatc gccgtgtaca tttgtctgcg tcttctttgc
     5641 tcatctccat cgttttcaaa tggatataag attacatgat caaattccag tccttttgct
     5701 agactcaaag gaatgatttg gcagttttct aatccatatg aatttttttt aatcgattct
     5761 tgtagcgttt gggtttcctc ttctgtttgg gtgataactg ccgctttttc tccttttaga
     5821 gaaaccaaaa tctcagctag tattttcaac aattcttcag aggatcgaaa agagcggtat
     5881 tcgggttctt ttccttgcgg tcggatcgat acgatgtcga tcttctcttg atccacagca
     5941 taggttttga aaagctctgt gattgctccg ctagaacggt aactatttct aagatcatac
     6001 cgatggatcg gcagatggta gtcgtcgaaa caacgcataa tatcagcaaa agtagtggaa
     6061 gaattaaaga ttgcctgatt ctcgtcacct actaaagtaa agtgagtttt tggaaataat
     6121 tcgatcaata gacctaattg tgccagggta taatcttgta cttcatcaat caatagatag
     6181 cgaaatgctg gtacatttac cttttcaaca agtaaatgac gaatagctaa tataatgact
     6241 gcttcatcaa cagtacgtgt gggttgccaa gcataagctg agccatatcg tttttcataa
     6301 attctttcaa acaactgatg ctcatccacc catgccattt cttcgatttg tctggttatt
     6361 tttcgatatt ttttttgtaa aagtttcctt gcataagcta caatactttg ttccgaatca
     6421 tcagaaatca atttaccgaa taattccaac tgcctatctt ctgtcaacga gagtacctga
     6481 ttctgcacag caggactttt tgcttgtttc aaaagatgat tcttccattc gctcatcagt
     6541 gcctttttcg ttgcttgaag cctatcgatc aatttaggat gagcaggaac tttctcatat
     6601 attttttcga tcgttttttt agaaaggact gtttttccat ttttagtaag tccacggata
     6661 aaattcggat gatctaaaaa caaaggatct gattgtttta gattatcgat aaactttttg
     6721 ctacgcaaac gttctgtctg ctccgataca ttttctcccg tgatacgttt gaaataagcc
     6781 tcttcacctt ctatttcctc tgctgaaagc tgtgaaacca gctgtatgat ggttagattc
     6841 agtggatttc tttcacctaa tgatggtagg acattcgaaa tatagtcgat aaaccgatga
     6901 ttaggtgata gaatcagcaa gtcatcggat gtcatcgtat ctcgatataa atagagcaga
     6961 tacgcaatac gctgcatgat agcagaggtt ttaccacttc ctgctacgcc atttaccaag
     7021 atattttgac ttttcacatc ccgaatgatt gtgttttgtt ccgattggat cgtagcagtg
     7081 atagccttca tttcattggt ctcatcttgg cctaagactt ctaataatac gtcatcttga
     7141 atagctacag acgtatcaaa aaacttgacc aaacgatctc tttccaaaag aaattgtcgt
     7201 ctttctttaa taaccgtatc gattttattt tgacgaaccg tataagaaga tggaccaatc
     7261 atgttattgt aaaaaagttc ggcaattggt gaacgccaat cataaacaag attttcatct
     7321 gcatcatctg tgaaatttgc tgtaccgata taaaaggatt cttttggctc attatccaga
     7381 aagtctacaa cgacttttcc aaagtaggga cttttcaata aacgttcgat ctttgctagc
     7441 tgattttctg cgtttttctg tttgatattc atctgatcga tttctctatt tttcatttca
     7501 atcatcgaaa aagtatccag gttatctaag taactgtcaa aattcaatcg aagctcgcca
     7561 gccatttctt tcaacgactg gtttccttct atttttgccg tatccatcga ttcccgcagt
     7621 tcgttttttt tatcaattag cttttgatac acgttcttca gatatgcttc ttctaactgt
     7681 ttttgtgtgt tgaccataca cattctcctc tttttttaaa gtggtggaac cgattagttt
     7741 acctctttta agagtcgaat gcaagctttt taacgagtat catttgttag gatatatcag
     7801 ttcttcaggt cggacagttt gatataaaga aaatgcacca tcaaggtttt ttacgtggta
     7861 gccagactgc ttcaagattc tttccgctag atagctacgt aatccgctat gacagctgac
     7921 gatatattct tgttggctgt ctaattcatt taatcggtcc cgaagttcat tcaaagggat
     7981 atgtttggct tccttgaatt tgcccttttc caattcttct gctgttcgca catctaataa
     8041 aatttttcct tttgctagtt ctgtcggcaa ttcatgccat tgtacggttt cactaagccc
     8101 ttctgcaatg ttcattgctg catatcctag catgtttact ggatctttgg ctgagccaaa
     8161 tggcggtgca tacgtcaatt ctaattccgg taaatcgaag atcgtcaaat gccctttgat
     8221 tgctgttgct aaaatatcga tccgtttatc cacacctttt gccccaacac cttgtgcacc
     8281 ataaatctcg ccagtctctg gatggaagat caacttcagt aaaatatctg ttgcttctgg
     8341 ataatagctt gcatgatctt ttcctgatac atgaatgact tcaaaagaca gttcgttttc
     8401 tttggctgtt ctttcactta aaccggtgga tgcggcagtc attccaaacg ttcgtataat
     8461 tgctgttcca ataccgcctt gatttcttcg agaaattccg gaaatcgtgt ccgcaacttg
     8521 tctaccttga cgattggctg gtgaagcaag agaaataagt gcatcttttc cagtgatttg
     8581 ctgttttaca acgatagcat cccccaccgc ataaatatca ggaatattcg tttgataacg
     8641 ttcgtctact aggatacccc cgcgtatgcc taattcgatt ccagcggtca ctgctagctg
     8701 gttatcagga cgaacaccaa tggataaaat aactaaatca gcagggatgg tacgcccatc
     8761 atccagtcgc aatcgtttcc cgtggtcttc aaatccagcg acagcatgag aagtaatcac
     8821 ttctactcct tgatgagaca attctttttc tataaaagct gccatttctt catccagtgt
     8881 tggaagaata tgtggtgctt gttcgatcac catgacttct aaacctcttc tttttaggtt
     8941 ttctgccatt tccaatccga tgaatcctgc accaacgatc acggcacgct ttggctgttt
     9001 ttctaatgca tgcatcactt tatcgatatc tggtacattt ctaatagaaa aaacattgtc
     9061 ggcttctgtt atccctggaa tcgatggaac aactggtttt gcaccaggag ataaaaccaa
     9121 tgcatcgtac tgttcaatgt gtttttgacc ctcatgtacg acttctaccg ttttgttttc
     9181 gggaaagatg gctgtcactt catgatttgg acgcacatct aaacaaaaac gagcggataa
     9241 tgattctggc gtttggacaa gaagattttc ccgttcactg atttctcccg aaagataata
     9301 aggcaagccg caatttgcaa aagaaacata cggtcctttt tcaaaaacaa cgatttctgc
     9361 atcttccatt aatcggcgaa gccgtgtagc agcagacatt ccacctgcta cacctccgac
     9421 aataacgatt ttcatcttat ttccctccta ctgttcgatc aaaccattga ttcatgccac
     9481 cacgtacatt cactacatca taaccagatt tctttaattc tttagctgct tgtttactcc
     9541 tcatccctga ttgacaaatc acatagacgg tcttttcttt gtctccatca tatctgttta
     9601 tactttgtaa tggaacattt ttagctcctt ttatatgccc gccccggtat tctgcaggtg
     9661 ttctaacatc aagtaacgct ggattttcca aaagcttttc ctttaattct gggactgtta
     9721 tcgattttat tgattgaaat agcaaattca ttttctgcac ctcatttaca tttttataaa
     9781 tacctcatgg ggtatattaa acaggagctt tattaatatg tcaaccagaa tgactcttca
     9841 tttttttcac tactttttat atttgcataa aataccgata aggggtatta tctaattata
     9901 aaaatagagg agaatgtgtt atggagattg ataataaaaa tatcctgaac cgtttgaaac
     9961 gtacagaggg acaaattcgt ggtatccaaa aaatgatcga agacgaaaaa gaatgtatgg
    10021 atgtcatcac tcaattaagc gctgttcgtt ctagtattga tcgcgtaatg ggcatgatcg
    10081 ttgcagataa tctgaaaaat tgctttgaga aacctgatag taatccagaa gaacaagcgg
    10141 ctaaactaga acaagcgatc aagatgatta ttaaaaagta aaggaatgat ttcaatgaaa
    10201 gtcgttttcc atattgatga attagaaaaa tggtcagaaa cagggaaaaa cgtaaaaaac
    10261 ctgataaaag cttctccaga aacgacgatt gtcgtatctg taaatggcat cgcgattacc
    10321 ggttatctag actctgccaa tgcagaattc ttagatttgc aaggagtcgt ttttcacgct
    10381 tgcgccaatg ctatgcgtgc taatcatatt tcagaaagtt ctttacctga acaagtaatc
    10441 gttgtaccag caggtgtcct tgatttagtt gaactgcaat cccaaggtta tgcttatatc
    10501 aagccttagt cagtagaggt tgcgacagaa gtgatcagct tcaagaaatt tcggcttatt
    10561 tttcgaagaa gctacttctg gaaaaccgtg aatcaaaggt tgtgaaaacg ctgtcctgca
    10621 tcaagtaata agaacagcca aacaaaaata gcttctcata tttttcgttt ggttgggctt
    10681 attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa atcagcggtt
    10741 tgacctaagt attagagaaa aaaacgggaa aatagctcct catattttgc cgttttttga
    10801 gctaataccg aaggtcagct gattgaacac cgtttaagga agagattgtg aaaaagtcgc
    10861 ttagtttcga gtattaagaa aaattttgtg aaaatggatt tctacatttt tgcataaatt
    10921 tggcttaata ccgagaaact ggcttttgaa taccgtttaa ggaagaggtt gtaaaatcag
    10981 cggtttgacc tgagtattag agaaacaaac ggaaaaatag ctgctcatat tttgccgttt
    11041 tttgagctaa taccgaaggt cagctgattg aacaccgttt accaagaggt tgtgagccta
    11101 ccatttaact ccgaatcaca agaaataatt tcaaaaacaa cttctcctgt ttttggttcg
    11161 gttggcttat tatcgagaag tagctactgg gagtctattt atcaaaaaaa ggaatgcgac
    11221 ataggtcaca cccctttttt attttctttg aaacatcttt cggtactcga cagcatacaa
    11281 gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa atgactctca cccacagcgt
    11341 tggtaattct gggaaaagca gcaaaaggat catgccaaca tagatgacag cagtcgctac
    11401 tttgccatac cacttcgctc cacttaattt gatattttta gatagcccga ttaatccagc
    11461 tacaaacata aagccttctt ttatcaagaa tagaccaaac aacagccaaa gccacggtcg
    11521 ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat ttatctgcaa acggatcaag
    11581 caccttccct aagtctgtta cctgattata tctccgagca atgaccccgt ctagataatc
    11641 agacaaacct gagacaacaa tgaccgctcc tgctatataa aatgactgtc tcagcacaaa
    11701 ataaagataa atcggcagca aaaaaatacg gaatatcgat aaaagattcg gaatcgtttg
    11761 tatttcttta cgccagtctg tgcttaattt cattttatcg ccctacctcc tcagcactct
    11821 tttcttttta ttttagctta cacggggaat aaaaccaatg atttcactgt ttttattttt
    11881 ctttacagac actgatatta tgagctaatt tgagataatc acttgctgtt ttcatcgttt
    11941 actcttcttt cttcctgtcg tttgcgcatt tcttttacta gtactttatg agcttttttt
    12001 gatcctcgct ctaggtcacg ctgccgttta cgtgttttat aatcgataaa cagactgtct
    12061 aaatcgtaac ctgtcgggta cagatcttct gctgaacgca ccagacgaac acgctgacga
    12121 ggcacgtgga ttattttttt gtctaaatat acatcaatcg tatcttcacc tatatcttca
    12181 taaactaacg cttctttttg gtattcattg acaaatatcc gatcaccttt tgcaaagagc
    12241 tgacgttctt ttttattctc cttcctattt gtaactgctg tcttaggaag aaattctttt
    12301 ttctgtgtcc gataggcttt ttccgccaag taacgctctg cttgatggat caatttcatc
    12361 gacatatcca ttttatgagc aatccacaaa gcttggcttt ctcctgtttc ttccaacccg
    12421 caactgatat ttaggacgaa gtgcttcccg atcgaaagcc atagcagccg gcacaaaatc
    12481 ttcatgatct ccggcgaatt tcttgatttc tccataatgg gtcgtggcaa tgatcaatgc
    12541 cccttgctca tacatagatt ccatgattgc gatcgctagt gcagcccctt catttggctc
    12601 tgttccactg ccaatctcat ctaaaagtac caacgtattc ctttttatct ttttcaaaat
    12661 agcagcgatg ttttgcatat gaccagaaaa tgtactcaac gcattttcta aattttggtg
    12721 gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc tctgtccctt cttttgccgg
    12781 aacttgcata ccgaacatcg ccatcaaagt caataaaccg actgtcttca acacgactgt
    12841 tttgcctccg gcatttgctc cggtgataat caagcctcga tagtctttac cgatttcaac
    12901 atccaatggt accgcatgct caggtaaaaa tggatgtctg ccttgcttga taatgatatg
    12961 ttccgattta ttgattctag gtgtgcttcc attgatttct cggctaaatt ttgcccgagc
    13021 aaagatgata tctaacaccg tgatcgtttc gatcaataaa tcgatcactt cttcgttttc
    13081 tgcgatagca ccagtcaatg ccgccaggac ttgatattct tcactgattt cttcagcttt
    13141 taacaactga tagtgttcat tcaatttaga aatcacagtt ggttcaataa aaaccgtcgt
    13201 tcttttattt gattcatcga taatcgttcc atctatttta tttttatagc tggctttgat
    13261 aggaatcgta tagtattcgc ctttttgaac aatcattgct tcttggatca tttctttgtt
    13321 tttcggatga cgcaagaatt tcagtaattt tgactggatt tctttttcga ttagttgtaa
    13381 ttgtttgcgt actttacgca aattacgtga cgcgtcgtca ctgactttat tattttttat
    13441 tttctggtca atcaactctt ctacatttat caaatcagga agatgtttgc tataagcaaa
    13501 caacaagggt gtctggtatt gattcttatc aaaaaacttt gtgatcatcc gactgcttct
    13561 aagaaaatcc gcatattcga tcaaatcggt tggctgcaga atcaaccctt ttttgacttg
    13621 ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga acatgctgac tgctttctaa
    13681 gatcaagcgt gcttctcttg tttcttcttg acgatccaag acagtttgta ggtttgtcga
    13741 gaccgtcata tcgctgattc gttttttgct atagtttcca attgctcttg cttggatttc
    13801 ctttttgatc cgatcaaatt gtaattgact aatcatttcg ttgtccatat tctctccttt
    13861 tcttgaagag atacctgtcc caaaataaat aagggcctag agataaataa aaggatagaa
    13921 cacagcttaa aaaagtcatg tcctatcctt attagaatac aaagatattg ttttcctgac
    13981 aggtaccgtt tcataagcac accaaataaa ctttgtttat tggctattcg acttactgat
    14041 tagataccag ctacactcac aaaacatacc ccattcttct aggtcttatt tattttccac
    14101 ttaactatag caagacacat tttggttgtc aaactttagt atgttttata gatttacacc
    14161 attaatgaat agacacctac cgcgataatt gctccgatca ttggacctgc gattggtacc
    14221 catgaatatg cccaatctga agttccttta ttagctattg gcaataccgc atgtgctaat
    14281 cgaggaccaa agtcccgagc agggttgatt gcatatcctg ttggccctcc taatgaaaga
    14341 ccgatggcta agatcaatcc acctacagcg aatacgttcg ttccaggtgc aaattcgttt
    14401 tgtccaaaag ctagcaaccc aagaactaat acaaatgtac caattaattc tgtgatcaca
    14461 tttgctggat aattccgtat agcaggacct gttgcaaacg ttcctaaaat cgcgctttca
    14521 tctttcgttg cattccaatg tggcaaataa gaaagccaaa caagaatggc ccctgcaaaa
    14581 gctcctaaaa cttgcgcaat gatgaaagga acgacaagat tccaactaat tgcccctgtc
    14641 atcgccatag ctagagagac tgcaggattc aaatgagctg ggcccatgaa tccagaaata
    14701 tagacagcca ttgttacagc gagtccccaa ccaaatgcga tgacaatcca gccagaagcc
    14761 tgtgctttac ttttgtttaa attcacagcc gcacacacac catctccaag caaaactagg
    14821 atcagtgtcc caataaactc ccccatgatt tgagtcatat cactgttcat ttatttctcc
    14881 ccctttagtt cccgcagatc tgattctgca atcaatgctt ctaaacgttt agtctgttgc
    14941 gccttctcct cttctgtcca ttcaaaatat ccagcaattg catcgatcac aggttgcttg
    15001 atctcatcaa gctgatctcg ttcaaacaac agatgatttg tacgacgaat gagataatcg
    15061 cctggggcca aaaccatttc ttcttctaaa ccataacgta accgagctga ctcagcgaga
    15121 ctcaaaccag gataaggtgc catttctttt gctaattcaa agatacgtcg ggcattagtc
    15181 ccgtaaaaat cagcgatata agtagcatct tcttcctcta aaccggctgc tactccttct
    15241 tttgctaatt ctgtcactgt ttcttctaat ttagttggat cgaagtttcc acctgaaatc
    15301 tgatattttt tagagtcgat ttctttcgtc tctattccgt attcttcttt taataattgg
    15361 cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat cggtgatttt cccaccagat
    15421 aaagtcagca aaccatctgg ttctctttct aaagaactac ctctggaaat cgtagaaggt
    15481 gctttttcat cacggctgtt ttccaaatgg ttcaacacgt cttctacttc agcacgagaa
    15541 actttgtttt ccttatattc acttactgta tcgacaactt tattgaagct cttgtctgaa
    15601 atcgatccat tatctccacc attgtaatca gaaccagaat taccaatcaa cagtggacga
    15661 agtcctgccc agcttgcttc gatatcattc aatgtgatat ttgcttctgg atagcgatga
    15721 ttgatcacat ccaatagata atctacatct tcttgtgtga ctttcggatc cgtaaaatct
    15781 ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt tttctcttgg aatagcaaaa
    15841 accatccgcc catcttgctt tcctgtatcg aagtatgtgg gttgcggtac aggcagtttt
    15901 ttcgcatcta caactaaatg gatcccttta gttggacgca ttttaggaga gactggacgc
    15961 gtaaaattca agtttcgtac tttatctacc caaggaccac tcgtattgat cactaatttt
    16021 gctttgattt cgatcacttc atctgtcagc aggtcacgag ctttgacgcc aacgatttga
    16081 tcgccatcat ataaaaagcc aaccgctttc attttactta ctagataagc cccatcttcg
    16141 gcagcctttt tgatattatc aatcactaaa cgggcatcat tgttgcggaa atctagatag
    16201 acacccgcgc cttttagccc ttcttttttc aaaaacggtt ctctttccaa tacttcttct
    16261 ggtgtcagcg tataattctc atatttggtt cctgtcacat ttgccagttt gtcataaagg
    16321 tccattgcta ctttgaccga gaacatattg aaggttgttg ctccttcgcc ctcatagatt
    16381 ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa tactttgtac gactgcacgt
    16441 tcaccaactg tgtcagctac tacttccaca tcaaatgtct tcagataacg aataccgcca
    16501 tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa aatcctgcat ttcgatcaat
    16561 cctgttttga ttcctgatgc tgctgactgg actgccacac cggcaccagt gattccgcca
    16621 ccgatgatca acagatccaa ttcttcttgc tgcatttttt gaatgctatc ttgtcttgtc
    16681 ttgtttgaaa acatatggat ttcctcctta ttcaaatgct tgggtagctt ttactgcttt
    16741 tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc atttgaactt caaatagctt
    16801 gccagcttca taaaattcgc ggatctcgtc tgtatctttc caataaccga cagctaatcc
    16861 tgctaaaaat gctgccccta gcgccgttgt ttccaagttt tctgctcgtt ggatcggtac
    16921 attcaagata tctgtttgga attgcatcag atattcattg tttgctgcac cgccatctac
    16981 tttcaataca ggaatcttga ttccagtgtc atcttgcatc gtatcgatga tatctctcac
    17041 ttgataagca atcgactgta aagtagcttt aatgatatct tcttttgtcg taccacgtgt
    17101 caagccaaac atagcccctc gtgctgcctg atcccaataa ggagcaccta gcccaacgaa
    17161 tgcaggaaca acataaacct catcttcgct tgtagagcgt ttcgctgcgt cttctgaatc
    17221 gctagctttt tgcaacattt gaagtccatc tctcagccat tgaatcgaag aaccagccac
    17281 aaaaatactt ccttctaaag catagtaaac ctttccattt atgccgtaac caattgtcgt
    17341 caataaatta tttttggata attgcggttt ttcaccggta ttcatgacaa taaatgaacc
    17401 tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc atttgtccga ataatgcggc
    17461 ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt ccataaaaat gataaccttg
    17521 cgtataacca taaacttctg agttagaagt gacttttggt aacatcactc gaggaatatt
    17581 tagtaaatct aaaatctcct gatcccaatc tagctgatgg atattgaaca acatcgtccg
    17641 actggcattc gagtaatcag taacatgtgc ttgtccatcg gtcagtttcc atactaacca
    17701 agaatcgatc gttccaaaca tcaattcgcc tttttcagct cgttcttgcg cgccttctac
    17761 atgatcaaga atccagcgta tcttcgttgc tgagaaatag gcatcgatca ctaaccctgt
    17821 tttttcgtgg aaaaattctt gatagccttc attttttaag ttatctgcaa ttccagctga
    17881 ctgtctggat tgccaaacaa ctgcatgata gattggacgt cctgtatctt tttcccatat
    17941 tactgttgtt tcacgctggt tcgttatgcc aatcccagca atctgatttg gtttgatccc
    18001 tgattcgatg aacgctccgg caataactga ctgtacagaa ttccagattt catttgcatc
    18061 atgttccacc caactttctt gcgggaaata ttgcgtgaac tctttttgag aactgccgat
    18121 gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta gttgtacctt ggtcaatcgc
    18181 catgatatac gttgattctg tcattgtgat tccctccatt atatgcaaac gctttcttta
    18241 actactctta tcatactact gttttaaaaa aagaaactgt caaaatttta ttcttttttt
    18301 ctttttttga cagtttttta ttatgaagct attttttaat caattctttt cttttttcag
    18361 cgtctctatt cttcttttca atcgaataat gtcgtaaggt gacgcaaatt ctgacagata
    18421 atattcacac tgttttttgt ttctctggca aaatgtgatc accaaatcat attctttacc
    18481 ttcttcataa cttccaatct caataccgcc gattcccctc agttctctga tcagatattg
    18541 ttgaaaagca atacgataaa tcggcagact ttctaaatcg attccaatca ctactggttt
    18601 tgcaatataa aaatcgatca tcaaaagaac attggcatac tggatcatca ggtaactaag
    18661 ctcactatct tcagctcttt tcctcggcaa ttgctgcacc gctgtttcct gtaatttcgc
    18721 cagtaactgt gttaaggaat gcccgagcat tttctgctgc tgttccaaaa gccgatcacg
    18781 ctcatagact tcgatctttc caacaaagaa aaaccattca ttgttgatct gggaaatttg
    18841 gtagcctact gccttttctt ctttgatttt caaacgacga ggacgataat gccataacat
    18901 cgtttcccga atgtaagtat ctaaaacagc ggttggtaac tttttgctcc gtgtcaaatc
    18961 atactgataa aatatttctt cgtctactat cgaaaaagaa acaaaaaagc tgtaaaacat
    19021 cattgtttcg tatacattca gttctgctgc tgttcgacta agatgcatca cgatgattgg
    19081 atcgattgct tgataaagcc ggtccgactg atagagtaat ttcttttctt tcaaagttgc
    19141 gtacgtactt ttttcattca atagtctttt tttcgtaatc ccgagccagc aagcaatctg
    19201 tgcttctgct gatgccgtaa acgttgtttc taatacacgg tccaatccta gaatgaacgg
    19261 tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga tcttccggga tatattggaa
    19321 tagttcataa taaaaatatc gtatctgtag ctcttctccg tataattgac cgtttttgat
    19381 ttgtaatcca aattcctcta agagctggtt caattctttg atctttcgaa aaacagaaga
    19441 ctcactgata ttaaacgctg tcgctaatcg aacgatggat acttgctgat gttctaataa
    19501 atagacgagc atcttaaact tcaagctttc ctgcaataaa aacaatagaa ttttttccaa
    19561 acttacatct tcgtccaaca caagcagcgc ctgatactca tttcgctgta cttctacttt
    19621 tttttccatc attcggccaa tcaattcaat atcttccaaa tattgatcaa acgaggcttt
    19681 tgacagttgg acatgctctc tcaaaccatt gatagaggct gttccccctt gcagaacaag
    19741 gtttcgcaaa atcaggatct gtaactgctc ttttctttct aataatgctt caatcttcat
    19801 aggcagtatt cgccaccttt gaccacacca tatctggcac acggtattct ggctggaaaa
    19861 atagctcatg ttcttctaac tgttcaaacg tccatccttc ttctactgca attgtcagtt
    19921 ggtcaagaag gtggaacatc tcttttttgc tttggagctg tgctccaaga atcttttttt
    19981 cagaatcaaa tagaaatttg agtttaactt tgctttgatt tattgctgta agcttgtagt
    20041 ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct ctcttcttct gtcaaaccta
    20101 cgcttcctaa gaaatagcca aaccactgat tgccgactgg acgataggtt ccctgatcct
    20161 ttgtctgtga gccactaatt gtcttgctga catttcgtgc tgtacggaca gcgttattta
    20221 ctagagaaac atacattttt tcatttgttg gacgaaaagt tacttggatc gcatcgccaa
    20281 tcgcataaac gtctttttga gacgtttgca agtagtcatc tacccagatc gttccatcat
    20341 cattcaatgt taattgttct tcccacatct gattgtcggg acgagaatga ttagccagca
    20401 aaacaagatc atagttctcc tttctttttt ccgtctctac aatagcggtt tcattttcgg
    20461 tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc ttgatttttc atttccttta
    20521 ctaacggttc tgtcatttca ggatctagat aacgaaaaag aattgacttt cggctttcgt
    20581 aaagatggat ttgttttttt cttgatgcca acccttcagc tatttcgatt cctgcttgtc
    20641 ctgcaccaat aattgcaatt ttctttgcat tcatgatctt ttgctgaaga gccgttacgt
    20701 tttgtatctt tttaactggg tgaataagcg ttgattctgc agaaatattt cgaaactttt
    20761 gatttgaacc agtcgctaga atcaaacgat caaaatccaa tttttttcct cctgctaaaa
    20821 cgactttttt ttctttcaat cctattactg tcttttctgt gtaaacatgg attcgatagc
    20881 ggtcagtcag ttcttctttc gtgatccaag aaagttgttt ttcactggta acctttcctt
    20941 ttaatatcaa attgaacccg ctgggaatcc atcctaactt cgattgcttt tcaatcaaat
    21001 atatttcact tgttggcatc aattggcgaa gataaatagc cgcatgaatc cctgcaaacg
    21061 acccgccgac aattacaatg ttcatctgca ttcctctttc ttataaggtg ttctactgct
    21121 aacttctcaa tcagaaaaca cagtattcat aactcgcttt caaccttatg atacatagcg
    21181 atgcagcaga atcaaccctc tccatcatac cgtgttctag gtctgcttct caataatgta
    21241 ctagtggacc taattgaaga gtatcatttc tgaagagatg tttcaaaaaa tgtactctta
    21301 taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa taggaaacag aaaagaacga
    21361 aaagacgagt tttagcgtgt gtaatgtaat agttagaata gacacatcat ttatttttaa
    21421 ttctttaatg cttcaacaac tgcttcgcct aattgatgag cagattgagg attttgccct
    21481 gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag ctgctccaga ctgatagttt
    21541 gctccttttt ctttcaaagc atcttctgct aagaatggta cttcattcgt tgtaccattt
    21601 gcttcctctt cttcattact gaacccagtc actgtacgtc cagctaaaat agattttcca
    21661 gactcatctg taactggcaa caatcctaca acaccgtgac aaacagctga gaccagccct
    21721 tcatttcgat agatttgtga agcgactttc ccaagatcta ttgcttctgg aaaatcccac
    21781 attgttccgt ggccaccagc ataataaacc gctgcataat catttgcatc cacatcatca
    21841 ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt catcttcata ataggcccaa
    21901 tcgatttcat ccatattcaa aacactttgc ggttctaaag gtacatatcc gcctttagga
    21961 ctgacaaaat ctgcatcgat cccatttttt actaatacat cataaaaatg agtcagttca
    22021 cttaaccaca aacctgtagg acgctcgatc ttttcgtact tttctacatt tgttaatacg
    22081 atcaatactt tcattttccc atcctctttc tataaaaatt gcgttattga taacgtgctt
    22141 tcaagaaatt atataatggg cttggcaaat aaagctcttt atatgctttt tcaaaaggtt
    22201 tcgttacaaa ttcatcctca cggttttcca ataatttctc tgcccagtca ggatcgacga
    22261 tcatttgctg tccaattgct gcaagttctg catatttcaa tacctgctct acatcttcgc
    22321 gattgcggat cccaccgaca ccaactaaag ggatctttcc ttgaatcgtg ttatggacca
    22381 cttcaagaat cgatttatcg ttgtatttat cagaacgagc tattcgatca tacatattta
    22441 atgaaacatg cagataatct agttttgact cacgtaatcg ttcaagaagc cagatcgtgt
    22501 cttcaaaacg gatacctggt gtttcatatt cttctggtga gaaacgatac cctacgatga
    22561 atggtttttt agcatattgt tcaaccgctg agaaaaccgt tgttaataat tcttcaataa
    22621 aacgataacg attttccaag ctaccgcccc attgatcctc tcgtctattc gaatgcggag
    22681 agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa ctcgattcca tcaaatcctg
    22741 attcaatcgc tctacgtgtg gctttgccaa atgcttggat tgtttcaacg atttcttctt
    22801 cactcattgc tcgtggtgtt tccgcatcag gacgttttgc tgcaactgca cttgcagata
    22861 caggctgttc tccagataaa gttgctcgac ttgtcattcg tccaccgtgg aaaatctgaa
    22921 taatcgcttt tgcgccttct ccttggattg ctttcgctag ctcacttaat cgaggaatca
    22981 tttcatcatg agcgatgctg agttcacctg gccagccttt tccgatatct tggacatttg
    23041 cagcaccagt aataacagca ccaagacctt tagaacgttg tttataataa gtgatctcat
    23101 cttcggtgat cactccattg aaaaagctga gttgtgtcgt cataggcgac agcatcaatc
    23161 ggttcttcaa ttgaactcct gaaggtaaag taattgattg atcaaattgt gacattgtca
    23221 ttcctcccat aattcgctct gattaaccta tcgagaatca tctatcatta aaccactcga
    23281 ttagttgtac atgcaactaa tttgcttaaa aaattttatt ttttttgtaa cttatctgtt
    23341 gcatcattca ttgtctgggc taattcttta cttgcttcat ttcccaaaac tgctttgtat
    23401 gactgatgaa gttcttccca gctaccatcg acttccttag caaattggcg tccttgttct
    23461 gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt tgacgtatcc tttatattct
    23521 aatttttcaa caaaacgagc aatcgttgat ggggcggaat agagcatttg tgcaatatct
    23581 ttttgtggta ttccatcttt ttcttttaat atcaataaaa taaaaccata agttggtgcc
    23641 agacctgtcc ttctaaacgc ttcatcagct aatttattca acatgcgatc tagctttttc
    23701 acgctaaaga aaagacattc atctaacgct ttttcaattg gcttttccat ttcatcccct
    23761 ccattggcgt acatatcata gtatgatttt tattttttgt caaaaaacac ggaataatgt
    23821 ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat cttcaaagaa tttcggcttg
    23881 ttttcgacaa aactccttct ggaacaccgt gaatcagagg ttgtgaaaaa gtcgtttagt
    23941 tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac atttttgcat aaatttggct
    24001 taataccgaa aaactggctt ttgaacaccg tttatatcag aggttgtgaa aaagccgtcc
    24061 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttgt cgttttttga
    24121 gctaatgccg atggtcagct gattgtacac cgtttattga gaggtcgtga gcctgccgtt
    24181 gaactccgag tcacaaggag caatttcaaa aaacagcttc tcatgtttta tgactatggt
    24241 gacttgtgat cgaggagttg gcaggagaat actgtttatt cgtaaagaag gggctgtgac
    24301 aagacttttg tcacagtccc ttatttcctt ttattatagg atttcgccat ttgattggat
    24361 gacttgtctg taccaatcaa atgatttttt ctttaaccgt ttctttgtgc cattcccttc
    24421 gtcatcttga tcaacataga tatatccata acgttttgac atttgtgaag tagaagcgct
    24481 gacgatatca atgcagcccc acgacgtata accaaataaa tctactccat ctttgatagc
    24541 ttccttcatc tgctcaatat gctgcttcaa atagtcaatg cggtaatcgt cttgaacggt
    24601 tccatcttct tctactttgt ctatagctcc gagtccgttt tcagctatga acagtggttt
    24661 ttgataccta tcatataatt gattcaaagc aattctcaaa cccttaggat caatctgcca
    24721 gccccattca cttgatggaa gatacggatt tttaattcct ccagttatta agtttccttg
    24781 gaccccttcc caattttctg gatgagcact gactgcacta gacatgtagt agctaaaaga
    24841 aacaaaatca acagggtagg ctgctaagat ttcttcatca cctacttcta tttttaagtt
    24901 gacacctttt tcttcaaaca ttcgttgcgt atacgctgga taatatcctc ttgcttgaac
    24961 atcactaaag aacaaagaag ttctttgctg ctcaattgtt ttttgaatat catccggatc
    25021 acaagaatac ggaacaagaa gctgatagct gatcatacaa ccgacttgtg aacctttgat
    25081 aatttcatga caagcttttg tagctaaagc acttgctata aactgatggt gtgcaccttg
    25141 gtatttcgct tgttccaaat ggggatggtt ttcttcaatt actcctacgc taacaaaagg
    25201 gtgatgtttt acacagttga tctcattgaa agtcatccaa tattttactt tatttttata
    25261 gcgtttgaat actgtttcag cgaaacgaac gtaaaactga ataagctttc gattaggcca
    25321 tccaccatat tctgtaacca aatataacgg catttcataa tgggaaagag aaacgactgg
    25381 ctcaatccca tattttagac attcgtcaaa cacttgatca taaaaaaata atcccgcttc
    25441 attgggtgtt tcttcatcac catgaggaaa aattcttgtc catgcgatag acatccgata
    25501 gcatttaaaa cccatttcag caaataaacg gatgtcttct ttaaagtgat gatagaaatc
    25561 atttccgtgt cttttaggat aataatactt gctgttggga tcctgcgccg cttttacttg
    25621 ctgatgcgtc atcatatgcc attgatctgt ccatttttct ttaggtaaat cgctgtcgaa
    25681 tgtatacaca tcagacacag ataatccttt ttcatctaca tcccatgcac cttcacattg
    25741 attggccgct gtcgctccgc cccataaaaa tccttctgga aattttttca tatcgatccc
    25801 cctaaacttg ctagttttct tgtttgatta tttttcttat gactagtaat gatatttttg
    25861 aatctactac tatttgtaac aataaccatt gtggtcggat catatccctc tttgattaat
    25921 tcttgcaaat ctacttccat caataaatcg cctttacgta ctttttttcc atcagatact
    25981 gcactttgaa agtatttgcc atttaaattc actgtatcaa taccgatatg gatcagtatc
    26041 tctgctcctt cttcactgac taccccaatt gcatgtttcg ttggaaaaac tgttgaaact
    26101 tccccgttta cagaactgta gatttttccg tccgttggaa tgatcgcaaa cccttctcct
    26161 aaagatccac tagcaaatgt tggatcgttt acttcagata aatcaattat ttcacctgct
    26221 gcaggagtac ttaacactat atcctttgag gattcttctt cgatttcatc cttgtagccg
    26281 aaaagaaaag ttagcataca ggaactgatc atcgctaaga aataactagc aactagccaa
    26341 acaaatcctt caccaatata aatcgggaaa gacaaaatac ttcttgtagc aactgccaaa
    26401 gcaccggctc ctgcggcacc agctactcca ccaaatatag ctccacatat acaagcaatg
    26461 taaaatggtc gtttatattt aagagtcaca ccataaacag caggttcagt gattccaaat
    26521 aatgctgtga taaatgcaga taaagataaa ctttttactt tcttattttt cgttttcaaa
    26581 aataccccaa gtactgctcc tgcttgcgca gctacggcag tcatacatgc tggacctagc
    26641 gtatcacgac cgtagaaaga aagattgttg gtcattactg gtacaagtcc ccaatgtaac
    26701 ccaaaaatca ctaacacttg ccaaaatccg ccgataacag caccagataa aacagggcta
    26761 aagttataaa cagctaaata tccagctgca atcagctgac ttgcataatc tgcaattgga
    26821 cctacaacta ataaaataat tgggaccatc acgatgatac aaatgaatgg tgtcaataaa
    26881 tttcgaatac tttcgtgcaa gcttttgcga atcttaggct caatataaga aagaacccat
    26941 acagctaaaa ttgctggaat aaccgtactt gtataccttg ctagaatcac gggcatccct
    27001 agaaatcgca aggttatgct atcactatat gcactgacaa tcgtaggata aacgagagct
    27061 gctgcaataa ccatagcgat gaaacgatct gtattgaact tttttgcagc agtatatgcc
    27121 aaaaatatag gcaagaaata gaaaaatgca tcagctgctg cattcaaaat gatataagtt
    27181 cctgattcag tagttaataa attcaaactc gtacataagg cgagtactcc tttgatcaaa
    27241 ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag cggcgatcat gtctataaaa
    27301 gagtttaaaa tgcctttttt ctcagttcct ctctttccac tagatgcatc atcttctagt
    27361 ttcatttgtt tggataatac gtcatacact tcatttactg catttcctac tacgacttga
    27421 aacattcctc cagcttcgac tacagtgatc acaccttcta gttgctctac cttctcctta
    27481 tctgccttct tattatcttt tagggtaaag cgtaaccttg ttgcacaatg gtaaacattg
    27541 ctgacatttt cttcgcctcc gacaaatcgg acaatatctt tcgctaattg tgaatagtcc
    27601 atttttactt cccccttgtt aattaatgcc gtagcaatcc ttcttacaag ataagtatag
    27661 ttcttagaaa acggtttctc cattcaatta gtaccccttc taatagcgaa cgaaactgta
    27721 caatagtgtg tatggtttac ttagccgagc aaatcactca ctgcttgaaa gaaaacaagt
    27781 atatcgtttc tcttatagtc aaaggagaca aaaaaaacac gcttgatctt cctttgctga
    27841 tgtataagtg gtatctcaag ctgatactct gtgatttttg tttctacttg gcttttgata
    27901 agacagcaca acaaagtctc ggcatttaac ggaagcataa ctaggtcaga actaggagag
    27961 attagttgct gaatggaatc tatcttttta tggattttta tttgattttc ttctttgtag
    28021 taatgatcaa gcagttttgt tttctttttt tcttcaacta tggtcagctt ttcacgcaaa
    28081 atctgataaa tctcccaaga ttcaaactca tctagaacac taggtagata aatcaaatca
    28141 tcccactcaa aaataagctc ttgctcagta gtcaaaattg catcatattc cgttattttg
    28201 tttgattgat ttttgaaaag atacactggt tctatccggc attctttgat ccacatgttc
    28261 atactttgtt gaatctgttt ttttagtgta ttcaaaatac taattggctg gttactaaca
    28321 atcagtatct ccccttgttt tcgaaaagga ttcaacgctt cggcaagata caaaacaaaa
    28381 taagcgcttt cagctaaatt gggacgtaat tgaaaatgta ttgggaagaa acgccgaatt
    28441 aaataaattt ctaaaggata acgggagatg atcttttgta caaaatgatt catggtatta
    28501 tgtccggcat ccatcctcgt tttcatttgt ttgatatggg ttagcaaaaa ttctgtttca
    28561 tggaacaata acgaaacagg aagatctaaa aaacgaataa tcgtttgctc aaatgctctt
    28621 agattcttct ctatttcttt gtttttttca tcattaatca tcaaataatt taactctaat
    28681 agtcgatttt caattgcttg tttttcttga ttggaaaaag aatagggtag ttctgatgat
    28741 aacttttttg tgagggtctc taccatcgga ataaataatg tatacctgtt tgttttttct
    28801 aaaagttctt ctcttcgaag aatggtcaaa acgagatacc gagaaaatcg tatgaagtcc
    28861 tctcctgaaa caatataatt atatgatata agaacctctt ttaatgtctt ttggacagtc
    28921 ggcatgatat tggaaaaaat tgtactgatt tgttgaggaa accttgcgtg ttggatcacc
    28981 gtttctgttc caacaagaga taagaaaatt ctttttgaat tttcttcagc aatgattttt
    29041 agtccttttg tcgacgaaac ttccagttct aatttaggat tgcgctcgat ccaacgcaaa
    29101 atcgttttta tttgtaatga tacggctgtt tttgaaagaa agaaaatgtc tgctatcttt
    29161 tgcatactga catacccttt ttgaaacaat aagtacgtca caatagttga agctcggaaa
    29221 tccatgctgg aatagatttt atgtttcgtc acctcttgta tgatttgctg tttagtttca
    29281 gattcaattg attctaaaaa ataacctgaa cgtttatttg actggataat gattttcttc
    29341 tcgtagatag caagcatatt tttgatttca gtttggatcg ttttcttgtt ggtacctaaa
    29401 gcttcagcta attcttcgct tgttatccaa taattcttat ttcttaatac tacaagaatt
    29461 tggagttgtc gtttatttaa gtccatttgc ttacacctcc tctttgttat aaacatacag
    29521 gaaaaacctc tcaaatgaca atagtcactt gcagaggttt aaaaatcatt tgaagaaaga
    29581 tctatattca aggtttttac cattagactg gataatttgt ttataatatt caaaactatc
    29641 ttttttcaat ctcctaaagc ttccgctccc atcattttgt ctatccacat aaataaagcc
    29701 atatcgcttc ctcatttccc ctgttcctgc cgatacgata tcgattggtc cccaccataa
    29761 gtaaccaatg atttggcaac catcttttat tgcctcagca atttcctgaa gatgtatttt
    29821 tagatactca caacgttttg gatcgtggat tcgattcaat tcatctggtg cttcatctag
    29881 tccaatgcca ttttcaacta tgaataatgg taattcataa cggtctgtca attcattcat
    29941 gacataacga aaccctagag gatcaattgg ccaactccaa ggttcgggag atttttcctt
    30001 caaaaaggga ttatcgaaac catttgctcc gccagtatct acgcgcattt ctgcttcttt
    30061 tttatagaca gcgctacgat aataactaaa tgcaatataa tctgccgtgt tagcttgcaa
    30121 gcactgttcg tctccatcta acattactgg tgcacagtct tcttccttcc atgtcctgta
    30181 aatataagct ggataatgcc ctcggcacat gacatcggag aaaaaccatg aattgcgttt
    30241 aaattgcagt gaaccaaata cattctctgg atcacagtca tatgcataag tcgctaatgc
    30301 agaaaagcta cacataatac ccatcttagc atttggatcg attttcttta ataattgagt
    30361 agcacgggcg ttcgctagaa acatatgatg acaggcttga taaatcactt tttttgttag
    30421 tgtagcaatg ggttcaagtt tatctctagc agggaatgta accaatccag cagctgcata
    30481 aggcatacga aaagcattat tgatctcatt aaaggtcatc caatatttca ctttcccttg
    30541 gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag tcgattaatt ttcgattttg
    30601 ccacccccca tactctccta ccaatgctaa gggggtttca taatgactaa gagtaattac
    30661 tggctcgata tcatatcgat ttaatgtatt gattagtctg tcataaaata cgagaccagc
    30721 ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt gcccatgaga tgcttgttcg
    30781 aaaagctttt aaacctaact ccttcagtaa acgaatatcc tcttcaaatc gatgataaaa
    30841 atcaatcgct tcatgagaaa gataatggag tgattcgtct aaagcaatct cccaaatctt
    30901 tttttcttca ttccactgaa tactaggatg ctggttcatg atcccgataa gcgtatctgt
    30961 cacattaggt aatttgccgt cttccaacca tgcaccttca cattgattag cagctactgc
    31021 tccgccccat aaaaaatttt ctggaaatat ttcctgatcc ataactatct ctcctcgcta
    31081 tcaacaattt cttacttgaa gtatagctcg attcatcaaa acaagatatt ctaactattt
    31141 cggcagaaga gaagaaaaaa attgaatgct actcttcatt tttaatattt atatataaca
    31201 aatacataag acgatccccg aaagaaaaca ctcgttaacc atctaagcaa acgatttatt
    31261 ttacttccaa cacaaatgct tcataaggtc gtaaatcaac tttgccgtct ggtaactgtt
    31321 tcagaggata atttgacaca ataaccctta aagcttcacg tggaatagtg aacgtctgct
    31381 tttcactgga catattacaa ataacgagcc attgctggtc ttcatatatg cgagaatagg
    31441 caaagacatg ctcaggcgtc ttttccacta attcatagtc tccccagacg atgatgtcat
    31501 gttttctacg caaatcgatc aatttcttgt acatataaaa aacagaatct tgatctgcta
    31561 acgcttcttc tgcattgatt tctttataat ttggattgac tcgaagccat ggaattccag
    31621 tagtaaaccc agcatgttca cgggcgttcc attgcatagg cgtcctagca ttgtctcttc
    31681 ctttcgcctt gattgattcc aagatttcct cttttgtaaa tccagaagaa atcctttcgt
    31741 tatacatatt gattgtctca atatcttttg cttccgcgat atcatcgatt gggcaattcg
    31801 tcatgcctaa ctcttctcct tgatagatat aaggggtgcc tttcatcata tggagcaaga
    31861 tagcaaacat cttggcactt tcttttcgat acacatcgtc atttccccaa cgagaaacaa
    31921 ttcgcggaag atcgtgatta ttccaaaata aactattcca tccttcatca cctaaagatg
    31981 tttgccattt ggataatact tttttcagtt cagagatact caagggtttc aaatcccatt
    32041 tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa ttggaagacc atggataatt
    32101 catttctttt aggattggaa taaagtttag cgatttcggt agttgctccc catgtttcgc
    32161 caacagtcat cagatttttg tcaccaaaag ttgcttgatt catttcctga agatactcat
    32221 gcagtttagg tccattactt gtgatttctt tatccggttc tttgccaatc atatcgatca
    32281 cgtccatacg aaagccccca atacctttat cgatccagaa attcatcatt tgataaatct
    32341 cttggcgaac ctgtttattt ttccaattca aatcaggttg acgcttgcta tacaagtgca
    32401 gaaaatattg ccccgttttt tcatccaact gccaagcaga accactgaat attgagcgca
    32461 atctattagg ctcttttcca ttgaccggat cacgccaaat ataatagtcc cgataaggat
    32521 tttctttatt tttacgggcc tcaatgaacc acggatgttc atcagaagta tgattcacca
    32581 ccagatccat gatgatacgg atatttcttt tctttgcttc ttcaatcagt tcttccattt
    32641 cttccatcgt accgaattct gccatgattg cttgataatt actgatatca tacccattgt
    32701 catcattcgg agactcgtat actggagaaa gccaaattgc atcaatacct aaatacgcca
    32761 gataatccaa cctggaaata atcccccgca aatctccaat accatcttga tttgaatctt
    32821 gaaaacttct tggatagatt tgataaacaa ctgaatggtg ccaccatttt tgttccatga
    32881 ttttcctcct ataaatggat gtttctattt tttgcttaat catttatttt agtctctctc
    32941 actaaaaatt gtaacgcttt caaatagcta ttgcaccttg ttatcggtaa cagaataaaa
    33001 caaaaaaaca gctgaggaaa ttccctcaac tgttttttga aaggattgat ttatgcaggg
    33061 aagaatgctg atttcacaac ttctttataa acggtgttca ctcagctgtc ccttggtatt
    33121 agctcaaaaa acggcaaaat ataagaaact attttttcgt ttttttctct aatactcagg
    33181 ccagaacgct gatttcacaa cctctttatt tcacattcaa tatccacatg aatcccacaa
    33241 atacaaagaa cagaccatac atgattggag atacttcttt tcctctttta gctgctaaca
    33301 tggtaattgg atagaagata aaacctaatg caatcccatc tgaaatacta taagtcaaag
    33361 gcatgccgat caagatcaag aatgaaggga tcgcaatctc taagtttccc cattcgattt
    33421 gtcgcaaaga ttgtgccatc aatactccta ccataatcaa tgctggtgca gttacttgtg
    33481 aagtaacaac tgatagtaat ggagaaaaga acaagcctaa caagaagaaa atccctgtag
    33541 taatagctgt gatacctgaa cgtcctccaa cggcgatacc agctgatgat tctacaaatg
    33601 caccaacagg agaagtacca aataaggaac ccgcaagcat agctgttgaa tcagaagcta
    33661 aagctttccc tacacgaggc atcttgttgt ctttcatgaa acctgcttgg tttgctaatc
    33721 caactaaagt tcctgctgta tcaaagaaag tcacaagcaa gaatgtcagc actacgaccc
    33781 acatttgaag agaattgatg tcccctacat gttttaatgc aactaaaaat gttggtttca
    33841 aacttggtgc tgcggcaaca atttcagaag gcattggaat caaacctgtg accagaccta
    33901 aaatcgttgt agctgccatc ccgatgaaga tgccacctgg tacacgccga acaagcaaga
    33961 tagcagttac gactaatcca aaaatcgtca accaagtaga accaacgttg agtgatccta
    34021 atcctactaa tgtagattca ttcgaaacga tgatcccacc ttcgctcaga ccaaggaaag
    34081 cgataaatag accgataccg ccagaaatcg catatttcaa atctgctggg atcgcatcaa
    34141 tgatcatttc ccgcagcttg aagattgtga tcaaaataaa aatcaaagaa gcaacgaata
    34201 cgccggctaa tgctgtttcc catggaatcc ccattcctaa acaaacagaa taagcaaaaa
    34261 aagcattgat cccaagagca ggtgccgttc caattggata acgggctaat acccccatta
    34321 aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa cactgctcct tcatccattc
    34381 cagaagcacc taatacagtt ggatttacaa ataagatata agccattgag ataaatgttg
    34441 taaatcctgc cagtacctca cgttttaccg tggtgttcaa tttttctatt tcgaagtagg
    34501 aaagtatttt ttccttcatc ttccatcccc ctaaatccga acattataaa taaagtccgt
    34561 taaaatttcg atttattaat aaaacaacgt gttttatcat atactaaaac aaaacataag
    34621 taaacaaaaa attcggattt taagcaaaat ccgaacgttc catctgatta aatcccttta
    34681 tctcaaagtt catgaacatt cactttggat tacaagaatt tctttattgt gttaataaag
    34741 tttcttcatt ctagtctcaa aaatcaatag tgtgatttga ttttaccatt tcgtttctgt
    34801 tcttctttga tgaattcttt tgtatcgaca ggattcagat tatttagtcc gccaaaacct
    34861 gtatcgacca tttttcccca gccttttgtc ttcttttttc ctgatttgag attctttgct
    34921 cgaagcttct cactatgttt tgctcgccga tacttcaaat aaccttctaa tcttcccatt
    34981 agatccactg tttttcctcc tttttattca atcagacgac tcagcttttc ctataacaaa
    35041 ataattgcct tcagggtcag aaaaattgaa agtcaagatg ccattgttat ctgtgatttc
    35101 tcctggttct tccatgttct cgtagatagt ttcaaattca tccgtaaaaa atactaaaga
    35161 aggcggtggt cctaatacct ctggtgaaaa cttctgcaca aatacttttg ggaatacgcc
    35221 taattctaat tcttttgaga tgctgagtac aatactctta aattcttctg gcaattcgat
    35281 tgtttcagaa atttctgcac caattttttc tacaaagaat cggcaaatca tctctacgtc
    35341 attgacataa agcatcacac gtgttttttg tatcatcatt tgacccttct ttctatttga
    35401 ttttctttcc tgtctataaa ctgattccat tatatcacga aagcgcaaac accttgtgtt
    35461 tgatactatt caagaaaaat aaagcaatag tttctaggat tgctttcctc cacaaacttt
    35521 ttttacaaaa aaaaaacggc aatgaatgcc gctcttctta tttcgattct tttataattc
    35581 tgcttctgat tcatctttgg ttagtgtgtg tctaacacta gttacgccaa atgtttgatc
    35641 gattttttcc ttcaacgctt cgattgcctg tgcgccacgt gccgcttgtt cagcattgat
    35701 ggattcaatg accagcaccg ctgcttgtgt ttcttccgtc agcatcgttc tttgggcttc
    35761 acgccagttg agacagcgac agatcgctcc ttgttcatca tagtagatga tttcttccgg
    35821 aagtgccgga gcatcggaat ctgcacctaa aggcaggaat gattcgccac cttgggcttt
    35881 tcctaaatgc agatttcctg cgatcttttc gatatcttct ccgccgcaag gtacgccaaa
    35941 agacaaggaa atactattat agatatctac aagcggattg attgggaaaa attcccgttc
    36001 ttgatttgca cgtttcagta aagcttcaat agaagaacgt gcaccttttt tagtcttgaa
    36061 tgtggtaaat gcctgacgcc actgagcaat gacttcgttt tggctgaatg tttcttctgt
    36121 aaggtaattt cttgcttctt ttgctgcctc attcaataat tgatgatacg tttcttcact
    36181 ttcttttgtt acgtggttat cgataccatt tacggataag atttcgatgg ttgcttcagg
    36241 aaatacttcc cagaaattgg tatccactat tacttttttc atctgttcac acctcttgat
    36301 tagaataatt gcttgatgaa tagatagtct gtttggggat catctcccat ataaaaaaca
    36361 tgttcgctgt atttgataaa tcctttttta cgataaaaag cgagcgcaac atcgttgtgt
    36421 tcccaaacac cgagccaaat ggctgttttt tgcttttctt ttgctttttc aatagctgta
    36481 tctagtaaaa atgtaccgat accttgatgt ttaaattttt ttcgtacata gattcgctcg
    36541 atttctaaaa aatgatcagc catttcctca gattgactcg ttcctgtatt tagtttcagg
    36601 taacccgcag gctcttgatc gatactcact aggaaaaaat cggtttcttt atcttttagc
    36661 tcttctgtaa gtttttctag tgcataagct tgtgtcaagt aatcagctaa atgctccggt
    36721 gaattatctt ttccaaatgt atctgtaaat gtttcgatac taatttgttg tagtaagtct
    36781 acatctgaag gcgtcatttt tcgaatttcg tatttattca cggcttcttc ctttcaatag
    36841 atccttttat tgcctttttt gacaaaattc caatcttcgc taacattctt gcatacttta
    36901 tttaaaagat attccaattg ttttgcttct tcgtcagaaa gaccatttaa tgcgactgca
    36961 ttggagtagt tgttttctcg aatgatgaaa ggatagattt ctgctccttt tttagtcgga
    37021 tagagtttct tgattttttt attttcttta tcttccaaac gttcgatcat cccattggat
    37081 tctaatttct tgattgctcg ggcagtcgtt gtacgatcta ctttgatcat ttctgctagt
    37141 ttttcttgga tgattccagg attttcgcaa atacgtacta aataaagata ctgtcctctt
    37201 gttaaatcga cttctttgaa ttcgatattg ctaatagaat ctagcgctcg ggcaatcatt
    37261 ccgatgtctc gaaggatttc agccatttca tcccaccttt attttgtttg taagactagt
    37321 tcacctcttt ttgttgcaaa tgcaactatt ttcaataaaa aaatcaagtt cttttttaat
    37381 aactagtaaa gtatattctt ttcttctttt acgaaaaagc tcaatcacaa tagatctggt
    37441 ttttgttttc taaaataagt aactgtttct tcgttgttcg cttttctgat agctttggca
    37501 actcttctat actaaaaaat cccatgttgg aagtttcatt attctcaata aacttggcct
    37561 cttcaccgtg gatcgcacaa gcaaaaacaa gtttataata ctgaaaaagc tgtggaatat
    37621 ctttttgttt attcgtatca aaaactgctc gcaactgagt agtttctact gttaatcctg
    37681 tttcttcata gacttcttta cgcacatttt cttcaggaga caatcctatt tcagcaaatc
    37741 caccaggaag tgaccattct ttcgtccgtt tatcttcgat tagtaggacc ttgccatctt
    37801 ttttgatata ggcacgaaca tctacttttg gtgttggatg tccttctcca atcgttgtga
    37861 gcgcttttat ttcttcgaaa gtttcttttc ctatttcact gatcagttcc aatgaaatcg
    37921 tccggatctc ctcaaaacgc tctttatcaa atacatcttt tccataatat agaccagctt
    37981 gagaaagtgc gagtagtctt ttgtattgag ccaacttctt gttcattctc ttcccttctt
    38041 ttttagcaaa tgtatgacta tgtaatcaag tatagaagac tggtttaatt acggcaaata
    38101 aatataatga aatctgcaat aaataaaaga atgttaaaaa actcgagaac aaaagacatt
    38161 cgacttctct ggggctgtga ataaatggcg ttcaaaagct tgcatctgcg gtaataagcc
    38221 ccaccaaacg aaaaatatga cgagctattc ttgtctggct gttcttatta attgaggcag
    38281 gacagctttt tcacaacctc cccactaaac ggtgttctcc taccaactcc tcggtcacaa
    38341 gtcacaatat tcatcaaaca tgagaagctg tttttttgaa attgctcctt gtgactcgga
    38401 gctgaacgac aggctcacga cctctcggta aacagcgttc aaaagccaat ttttcgatat
    38461 taagccattt ctttgttact tattgtcatt gatttctttt tctaatttat ccaatagtgg
    38521 agtgtaatcc acttgagtat agtctgttac atcaaattca tatagtgttc ctaactggaa
    38581 tttgtcatat tttcggtcgg cgatgatctt tttgaatgct tctttcgtaa tatgattggt
    38641 ccctttcgtt cgatcttcca atacgtcacc atagtgataa tgatccatga tatagctcaa
    38701 atgacgatct gattcacgat cacgtttgta ccgacgttgc cataacactt caaagtctgc
    38761 tgttaggcga atcgtgatca cctgatatcc gtatctatct gctaattctt ggagtcgtgc
    38821 tttttgctta tcactaaaag gatactcgga cacaatcact ttctttcctg cttccatata
    38881 caaatcaagt gctttgtaat agaaaggata aactttcgtc ctttcttgtt ccgcccgttc
    38941 ttccaaattg ttaaacccga tggattctgc atagtattcc ttgatttcat cgagcgtgat
    39001 ggcatacata tctggaaatc gttcccgtag tttattcata agatatgttt ttcctgtacc
    39061 aggactacca gccagaagaa tgaaatactt tttcatctca tttatttcct aagttaattg
    39121 gactttccaa gttcataaac agtccaatct tatgcttgac aatttctttt actgcttcat
    39181 ttgctggaat cacattatgg cgaagagact tgttgatttc tgtttcacta aaggtttctt
    39241 tagctacttt cgtccagttg actaaaagct ccgttccaac attgaatttg cggatcccgt
    39301 tattgatcaa ttcaggataa tcttcttctt tcacacctgt tcctccatgg ataactaggg
    39361 ggacatcaac tactgcatcg atttctttca gcaagggaat gtttacttct gtttttgatt
    39421 tgaattgtcc atgattcgta ccgatagcaa tagctaatgc atccacgcct gtagcttcta
    39481 caaatttcac tgcgtcttcc ggtcttgtat ataccttgtc attttcagcg acatggattc
    39541 cttcttctgt accgccgatt gttccaagtt ctccttctac ggaaacaccg tgcgcatggg
    39601 catattctac tactgctttt gtcttcagga tattttcttt gaaaggcaga tgggacccat
    39661 catacatgac agaagtatat ccgctgtcga ttgcttcttt gatatcatca aagtctcggg
    39721 cgtggtctaa gtgcaagact gcatcaacca tttcttcttc tgcaatagct gtcattacat
    39781 tgactaacac atgcatgccg atatatttag cggtatcgac acttgtttga ataatgatcg
    39841 gcgaacccat ttcttttgct gcgcctagca tagcaggaag catttctagg ttatgcgtat
    39901 taaaagctcc aactgtaaaa ttcaactctt ctgctgtttt tgttacttct ttcaatgttg
    39961 tatacatctt ttattgccac cttatctttt tatttgtttc ctttgatcgt tacttgtttt
    40021 gcaatttgtc gcatctcatc cattttgccc atgtagtaat cgatcccttg atcatcacca
    40081 gcacgagcag catctgcacg cttctggtta tagaggctca tcagtttttt atacccatct
    40141 ccgatcgtct gtttattttc caaactcttt tctaaatatt ggatggcttg atcgttcaca
    40201 tacaattcag aaaatttaag tccaacttgt tcataaagtt tcgctaattt atgcttatct
    40261 tgggtagtgg aaatctcttg tttcaattgt gtaatctctg attgaatctt ttcggtcgct
    40321 tctggcgata gggctttttc ttcttttgcc agaacttttt tcggttcttt ttcttttttc
    40381 ttaaaaaatc caaacatcat tttccctact ttcaattaaa atgctttcaa aactgggctt
    40441 aagatccagg catataagag tgctgcggct actgtatcta catcggttgc tgtagcattg
    40501 ttgaatccca tcgaattaaa gatcgttact agcaaagcgg gcaataatgt aataaagaaa
    40561 ccatgagcaa taccgccaat gatcgctcct cggcgtccgc caactgcgtt gccaaatatc
    40621 cctgctgtcc ctccggcaaa gaaattggtt agcataccag gtaaaatcat cgctaagccg
    40681 aacatgggca aagtaaacat ggcaataatc gtaccaatcg ttgtcgtaat gaaacctaaa
    40741 atgacagcat ttgggctata aggaaagaaa actggacaat ctaaggcagg gatcgcatca
    40801 ggaactaatt tcatagctat ccctcgaaat gccggcacga tttcacctaa cagtagacgt
    40861 acaccagcta gcaatacata cacacccact acgaattgga tggcttgtaa aaaggcaaac
    40921 atgatatagt tttgatctcc tgataattca gaagcgaatg gttcgcctgc aaataaaaca
    40981 gtgatgatat atagcgggac catcacgacc atgacagaca aataagtatc ttgcagaaat
    41041 tcgaaatgtg tcggcaactt gatatcttcg atcgactttt tattttcgcc tttctcacca
    41101 aaaagcttag caacaccggc ttcaaataag tacccgatcg tacaaaaatg acctaaagca
    41161 atatcatttg atccagtgat tttacgaatg atcggttgcg ctactgccgg catagcaatg
    41221 gcgaaacaag ctccgataaa accaccgacc aagatcaata cgatccctcg cagaccggca
    41281 aaatagccaa agactgtcgt catcgttgcc atccataaaa ttgcttgacc agttaaaaaa
    41341 atgtacttcc attttgtgaa acgagccaaa ataatattga atacaaaaat ggctaagaac
    41401 gtcagtgcaa tatctctacc taaacccaaa tcattcattg cttgaccatt gatagattca
    41461 atagaaggga tgatcccttg catatgaaat ccttcagtaa agattttccc gaaataagtt
    41521 aaacttccaa cgatgatgct cgaaccggca ctcaacactt gaaatcctag gagtgtcttt
    41581 aacgtgccag agatgacttg tcctgctgat ttcttctgca gtagtagacc caacatagca
    41641 atcaatgcaa tggtgatcga tgcctgagtc aaaatatttt cgatgataaa attgataacg
    41701 cccat
//
LOCUS       DASJTC010000012        40795 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.012, whole genome shotgun sequence.
ACCESSION   DASJTC010000012 DASJTC010000000
VERSION     DASJTC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.012"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            168..1309
                     /locus_tag="U4B19_000603"
                     /pseudo
     CDS             168..1309
                     /locus_tag="U4B19_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1435..2514)
                     /locus_tag="U4B19_000604"
     CDS             complement(1435..2514)
                     /locus_tag="U4B19_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2076365.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2617..3057)
                     /locus_tag="U4B19_000605"
     CDS             complement(2617..3057)
                     /locus_tag="U4B19_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2076366.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3114..3524)
                     /locus_tag="U4B19_000606"
     CDS             complement(3114..3524)
                     /locus_tag="U4B19_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN2076367.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3704..4138)
                     /locus_tag="U4B19_000607"
     CDS             complement(3704..4138)
                     /locus_tag="U4B19_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2076368.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4238..5086)
                     /locus_tag="U4B19_000608"
     CDS             complement(4238..5086)
                     /locus_tag="U4B19_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2076369.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            5475..7241
                     /locus_tag="U4B19_000609"
     CDS             5475..7241
                     /locus_tag="U4B19_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEN2076370.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7343..8602
                     /locus_tag="U4B19_000610"
     CDS             7343..8602
                     /locus_tag="U4B19_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2076371.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8749..10050
                     /locus_tag="U4B19_000611"
     CDS             8749..10050
                     /locus_tag="U4B19_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2076372.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10053..10904
                     /locus_tag="U4B19_000612"
     CDS             10053..10904
                     /locus_tag="U4B19_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2076373.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10917..11732
                     /locus_tag="U4B19_000613"
     CDS             10917..11732
                     /locus_tag="U4B19_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEN2076374.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11883..13049
                     /locus_tag="U4B19_000614"
     CDS             11883..13049
                     /locus_tag="U4B19_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2076375.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13279..13926
                     /locus_tag="U4B19_000615"
     CDS             13279..13926
                     /locus_tag="U4B19_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEN2076376.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14010..14954
                     /locus_tag="U4B19_000616"
     CDS             14010..14954
                     /locus_tag="U4B19_000616"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEN2076377.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15089..16540
                     /locus_tag="U4B19_000617"
     CDS             15089..16540
                     /locus_tag="U4B19_000617"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEN2076378.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16561..17397
                     /locus_tag="U4B19_000618"
     CDS             16561..17397
                     /locus_tag="U4B19_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN2076379.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17397..18056
                     /locus_tag="U4B19_000619"
     CDS             17397..18056
                     /locus_tag="U4B19_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2076380.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18182..18871
                     /locus_tag="U4B19_000620"
     CDS             18182..18871
                     /locus_tag="U4B19_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076381.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18877..19833
                     /locus_tag="U4B19_000621"
     CDS             18877..19833
                     /locus_tag="U4B19_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEN2076382.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19877..20389
                     /locus_tag="U4B19_000622"
     CDS             19877..20389
                     /locus_tag="U4B19_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2076383.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20645..21193)
                     /locus_tag="U4B19_000623"
     CDS             complement(20645..21193)
                     /locus_tag="U4B19_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2076384.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21282..22463)
                     /locus_tag="U4B19_000624"
     CDS             complement(21282..22463)
                     /locus_tag="U4B19_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEN2076385.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22699..24207)
                     /locus_tag="U4B19_000625"
     CDS             complement(22699..24207)
                     /locus_tag="U4B19_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEN2076386.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24366..24557
                     /locus_tag="U4B19_000626"
     CDS             24366..24557
                     /locus_tag="U4B19_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076387.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24760..25677
                     /locus_tag="U4B19_000627"
     CDS             24760..25677
                     /locus_tag="U4B19_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEN2076388.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25670..26743
                     /locus_tag="U4B19_000628"
     CDS             25670..26743
                     /locus_tag="U4B19_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076389.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26891..27409
                     /locus_tag="U4B19_000629"
     CDS             26891..27409
                     /locus_tag="U4B19_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076390.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27390..27782
                     /locus_tag="U4B19_000630"
     CDS             27390..27782
                     /locus_tag="U4B19_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEN2076391.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27858..28793
                     /locus_tag="U4B19_000631"
     CDS             27858..28793
                     /locus_tag="U4B19_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEN2076392.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28926..29705
                     /locus_tag="U4B19_000632"
     CDS             28926..29705
                     /locus_tag="U4B19_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN2076393.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29698..30015
                     /locus_tag="U4B19_000633"
     CDS             29698..30015
                     /locus_tag="U4B19_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN2076394.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30259..31869
                     /locus_tag="U4B19_000634"
     CDS             30259..31869
                     /locus_tag="U4B19_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2076395.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32026..32874
                     /locus_tag="U4B19_000635"
     CDS             32026..32874
                     /locus_tag="U4B19_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2076396.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            33105..33584
                     /locus_tag="U4B19_000636"
     CDS             33105..33584
                     /locus_tag="U4B19_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076397.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            33617..35845
                     /locus_tag="U4B19_000637"
     CDS             33617..35845
                     /locus_tag="U4B19_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2076398.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            35974..37617
                     /locus_tag="U4B19_000638"
     CDS             35974..37617
                     /locus_tag="U4B19_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEN2076399.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(37690..38235)
                     /locus_tag="U4B19_000639"
     CDS             complement(37690..38235)
                     /locus_tag="U4B19_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEN2076400.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(38258..38743)
                     /locus_tag="U4B19_000640"
     CDS             complement(38258..38743)
                     /locus_tag="U4B19_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2076401.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(38733..39545)
                     /locus_tag="U4B19_000641"
     CDS             complement(38733..39545)
                     /locus_tag="U4B19_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEN2076402.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(39684..>40795)
                     /locus_tag="U4B19_000642"
     CDS             complement(39684..>40795)
                     /locus_tag="U4B19_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN2076403.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        13542 a         7395 c         7977 g        11881 t
ORIGIN      
        1 atcgcttctt gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa
       61 atattactgt ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc
      121 tactatctta tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa
      181 ccatcttaat atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc
      241 acacagagga gacagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa
      301 ttacctttca ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac
      361 aaacagcgac catcaaagca aaaattgact attcgggaga cttagcacct atactacaat
      421 cagctgttac atttgagaat gttgatccca ctttgtcgat tggctatgac gagaacactt
      481 tgactatcca acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt
      541 taaaaccagc gctttttact gtcaaggtat cagatggact accgaacgat catcatttaa
      601 cgccgtatag tcagcggcag acaattgagc aagattcagc catcgactca ggaggagatc
      661 ttgttgaaga accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag
      721 aaaatccaga cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa
      781 ctcaaccaga gccagacaat aaatcaaaac ctgaaataac ccctggagaa aaaccagatg
      841 taactccaga accagatacg gattctggaa accaaacagt accggaaaca aatccagaca
      901 cagataatga aactgaaaat ccagaaaaac cagaggtaga tccagaagaa aaaccagatg
      961 tgactccaga accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa
     1021 aaacaattca agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa
     1081 taaatgaaga gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa
     1141 ccttaaaaag tagcaaggat acaacctttg ctgtcctgaa acgaaaaata aacagctacc
     1201 aaaatccgga gagtcacaaa acaaagttat actgtggagc ggaatcattc tattaagtat
     1261 agcaacaatg ctttctgcaa aaagatttaa acaaaacaga tctctctaac atttgaataa
     1321 tgtctgtgaa cgcatttttc aaataaaaag aaatatccag aaaatgaccc caaaatacca
     1381 gattgaaatc tgatatttgg ggtcgttcat actttatctt tcttcatatt tattttaagg
     1441 gatatctgtt tcttttggaa gatggttcgg gatctttttc actttcaata catctaaaaa
     1501 gaaggtaaag atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc gttcgctaac
     1561 aagtaagacc acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca tcaacttcgg
     1621 attcagcaca taagactcaa ttagatggac aatcaaaatc gtaatcagga tataaacaac
     1681 atctcggatg cctccttggg aataagctat gaaagataga ggaatacaag aaatgataac
     1741 acctgctact gggatcagac ttaaaatgaa aatcatgatc gctaaactca gcagctgatg
     1801 aaagccaaat acagccaagg cgattgtagt gagcaacgta tttaaaagag cgacaacaaa
     1861 ttgagcttct aataccaaac caaatgtatt gacaaactta tcggcaaaat agtaaatatc
     1921 ttgaaagaac caagaaaatt cactttttaa aaacaatctt gagaatacaa ttgttttttt
     1981 cttttcaatc ataaaaaaga aacttaagat gaatgacata gcaaacgcca ctgccatttt
     2041 tcctatatcc tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct gttcaaaaaa
     2101 atcgtttcgc tcaatataag tatggatata ttgtaaaaca ggatctgcat cttttggctg
     2161 atgctcgtaa aaattaataa cggactggat catgtcaaat gtttgatgaa taagcatcgg
     2221 gatatacttt gtgaatgcca gatagataag gtaaacgatc agtgtataaa tcatgatagc
     2281 taaaacacct gtagggattc ggacaaatcg ctgaatgatt tgaacaagat gaatcatcaa
     2341 ataagtaaag ataaatgtga gcaaaatggt tgtaatcata cttctaacta agaaaagaac
     2401 aaaaacgaca aataataaaa cagtgagacg gcgtattttt tcattcgcca aaaaacgctg
     2461 ataaacggtc aaatcgacaa cctccaaata acaaaaaata aacacgttat tcattatatg
     2521 atggaatatg aaaataagaa agagcaaaaa gaaaaaccgc taaatgacgt tatcttatga
     2581 gttggttttc ctcataaaat cgttcatcta gcagtttcag ccctttttct ggtcttgatg
     2641 aaaattatct agcatttttc gggtcagatg ttgcagtgta tccaattctt cctgttcaag
     2701 tggaagataa gacttgatct tttgtggaat accttctgct tgtttttgca acgcagcacc
     2761 ttgagcagtc agcgtaacat ttacgattct ttcatcaccc gtttcacgct gacgattaat
     2821 ataatttttt tctgctaact tttttagtaa gggagtcagc gtaccagaat ccagacacaa
     2881 atcatttcct agctctttca ctgaagtctt tttcttttcc cataaaacta acatcacaag
     2941 atactgggta taggtaagat tcagcggctt gaggagggga gtgtagtgtc taatgatttc
     3001 tttagaggcg gcatataaag gaaaacatac ttgattaact aaatatagat cttccatttt
     3061 tccacctccg ctgttttact atagtgtatc atagcggatt tacacaaaaa agtctattca
     3121 ctaactgctt tcagagtaac ttctatattt ccagctgtag cttttgaata cggacacact
     3181 ttatgcgctg cctctaataa ttcttgcgtt tcttccagag aaagaccttc gatagatcct
     3241 tcaatctcta cacctaagac aaagctcgtt tcactttctg aataaaggga aacttgtgca
     3301 ctgacagtag aagccccaga gatatttttt tgctgtttga tcaattctaa tgcactgtta
     3361 aaacatgcac tgaaaccagc tgcaaacaat tgttcagggt tggttgcatt ttcgacgcgt
     3421 ttaccaggag ctgcaatatt caattcgaat gtacgatcgg gattatgcac ttctccagag
     3481 cgtccgcctg tgttgatcat tttcgtagaa tacatttttt tcataaagtt accaccttta
     3541 attatatttt atacaattga attttacaca atataattgt ggaaagcaaa gaaatggatt
     3601 ggcataagat aaataagaga aagcacatga aacagacgcg ctttctcgaa tagcaaacat
     3661 atacaaaaaa tatcagagac aaaaaataat ctcatcataa acattacata tatttctcaa
     3721 aaagttgttt tctttcttca aaaattactt cacgatggac ctgatgaagc atccataaat
     3781 aaccaaaagg atctctgaac attgagttag tcacaccaaa gtccatcatt tctgtcattt
     3841 cttgaatctc tacacagcca tttcgtaatg ctttattatg tgtgtcctcg atatcagaga
     3901 cgatgatatt gaaccagatc gtatttggat gttcagcagt aggagcagtc aaaccaagag
     3961 acgtgttttc atccagcata tggaatctcg taccatacaa ggtaaaaatg acttcattat
     4021 tgcctctagg gtaatctgtt gcctcaatca gttgtatttc atcaaagatt gtttgataca
     4081 gttctagtgc tttcaaacta tcatttaata caaaatcgat ttccgttcct ttatgcataa
     4141 agatctcctc tccttttcag tcgaacagtt actagattct tttattatac cagctaaaga
     4201 aaacgcattc aaaaaaatag cggatttact taccaactca ttcgattgga tgataagtaa
     4261 atccgcgtcc cattacttct gctacgctgc tgacaatgac aaaggcactt ggatcgattt
     4321 cttctatcaa tggtttcatt cgggaaaatt catgatcagt cacaacgatc aataaaacct
     4381 cttttgactt tcgattatac ccgccttttg cctctagcag ggtaaggcca cgtccaatct
     4441 cagcacttaa gcgaaggcgg atgtcttcca aataatgttc actcatgacc ataacagatt
     4501 tctttcgatt gatacctgtt tcgatataag tcatgacaat tgaagtgatt gcaatagata
     4561 aaatagcata aagaaactct tctattccaa aaataaacag attaagagaa acaacgaatg
     4621 cgtctgtaaa aaataagcca atcgaagtat tcaagtggaa atatttttta aaaatgaggg
     4681 gagggatcgt cgtaccgccg ctagaagagt tgtttttata taaaatcgca actcctaaag
     4741 caaaaatcgc acttccaaaa atgacagaca acaaccgatc tgaagtaatc attgtttctg
     4801 gaactattgc aatgattaaa ggaaaaacca agcttccgaa aagtacttta aaaaagggtt
     4861 ttttacctaa aaatagaaga gctagtacca acataacgat attcaacact aaaataacta
     4921 tagctcggtc aaagcctaaa gcagcttcca gtaatattcc tagaccgctg acacctccag
     4981 cagcaatatg atgaggccct aaaaacatat tgatagcgac tcctaatatg aagagggcaa
     5041 aaataatctt aatgtatgtc catataaatt gtctcgtttc tttcatttat tcacttcctt
     5101 aattcattaa taatactctt atcttattat aacttacttt tcaccttaag ttaactttaa
     5161 ttaataaaaa aattaggcta gtctaaatta ttatgtttca ttcttctaga atatacttct
     5221 acgccaaaaa ctgttaaaaa acttttactt tagaaaattg gagaaattga tcgcttaaga
     5281 taaattcagg acactaaata gtacaggaag tagactaaaa cttttaaaag ttttaagtct
     5341 atctatttgg caagtttaaa aaataaaaat tcggtatcaa aaaactgtta ccgataacat
     5401 ttttgaaagc gttgtctttt gcttctctat cacttatagt gatgacgtca tattaaaaca
     5461 caaggaggaa atcaatggat acagctgcaa tttatcatcg accggaaagt gaatatgcat
     5521 ttctatatac gaatacaaga tttcgtattc ggcttcgtac taggaaaaat gatatcaaaa
     5581 aagtatatgt gctatgtgga gatccatata ccataacaac tgaaaaatgg tatcaaaaac
     5641 aacgaccaat gaaaaaatgt ctaagtacca atgttcatga ttattgggaa attgaagtga
     5701 cgagcgaaac aagtcgctta cagtatgcgt tccatgttgt tggggaagat aacacggact
     5761 gtttttacgg tgatcaagga atttttcctt atcgagaaaa tgtgattact gaaccaaatt
     5821 tttattttcg aataccttat tttcatcaaa tcgatcgatt cactattcca gaatgggtaa
     5881 aagaaacagt atggtatcaa atttttcccg aaagatttgc aaacggggac aaagcaaatg
     5941 accccgaaca cactttacct tggggaagta aagatccaga ccgagaagac tttttcgggg
     6001 gagatttgca aggtgtgatg gatcatttgg attacttgac ggaccttggc atcaatggca
     6061 tctatttttg tcccatattt aaagccacat ccaatcataa atacgatacg attgattatt
     6121 atgaggtgga tcccgctttt ggcgataaaa aacttttaaa aaacttgatt gatgaagcac
     6181 ataaaagagg gatacgtatc atgttcgatg ctgtattcaa tcatatgggt gtccattcac
     6241 cacagtggca agacgttctc aaaaacggag aaaaatcgat ctataaagac tggttccaca
     6301 tccgtttttt cccagtagat tcttatcaga tgactgactt accagaaact gctgaaaata
     6361 tcccatatga tacttttgct tttactccat tcatgccgaa actaaataca gcaaatccag
     6421 aagtgcaaaa atatttgctg gatatcgcaa cttattggat aaaagaattc gatattgatg
     6481 gatggcgtct tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa ttcagagaag
     6541 cagttacaga aatcaaacca gacatctata tccttggaga gatttggcat tcctctcaag
     6601 cttggctgca aggggatgaa ttccatgcag tgatgaatta tgcatttaca gattcgatta
     6661 aagattattt tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga atgaatcatc
     6721 agcaaatgct ttacagagat caggtgaacg aaggaacatt caacttatta gattctcatg
     6781 atactgccag gatcttgacc ctttgccaag gtaacaaaga attgatgaag tctgtgatgg
     6841 cctttatgtt tcttcaaaaa gggtctcctt gtatctatta cgggacagaa atagggatga
     6901 ctggtgaaga tgacccggat tgccgcaaat gtatgatatg ggagaaggag gaacaagatc
     6961 tagagctatt cggttttata aaagaacttg tttctctaag gaaacagttg tctaagatta
     7021 tttctgaagg ttctactcag tggctaatag taaatgatcg tgaagataag ctctatttca
     7081 cacggaaact agagggacag atcatctatg tttactttaa tcagtcaaaa gaaccttggg
     7141 tagtagaaca ggaaaatgag gtgattctta gtcaaaattg tcagttgtta gaagacggga
     7201 aagcagagat acgacaatac ggatttttga tcatgtgttg aaaactgtta ccggtaacaa
     7261 caagaaagcg tttgcaaatg aagatttgtg cccattataa taagggtgta acagcacgtt
     7321 gaatacaaag gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg gtttagggtt
     7381 aatctcttta ggaatcttag gcggattagc ggcttgtggc aatggtaact cttcagcaga
     7441 aggggaagcc gatgaaggaa aaacgttaac agtttccgtt gattctggat ataaagatta
     7501 tataaacgaa ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca agctagtaga
     7561 aaaggacatg ttcgatcagt tggaatcatt agcgttagat gggcctgctg gaaagggacc
     7621 tgacgttatg atggcagcat atgaccggat cggtgcgtta ggacagcaag gacatctggc
     7681 ggaagtaaaa ctgggaaatg aagcagctta cgatgaaaca gacaaagccc aagtgactta
     7741 tgatggaaaa atctacggag aacctgcagt aatcgagacc cttgttttgt actataacaa
     7801 agatttagtc gatacagcac catcaacgtt taaagatttg gagaatctat ccaaagacag
     7861 tcgttttgct tttgagtcag aagcaggaaa aaatacagga tttttagcta aatggactga
     7921 cttttattat tcctatggtt taattgcagg ttacggtggc tatgtatttg gtgatgatgg
     7981 cacaaatcct tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa tctcttatgc
     8041 aacagattgg ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa gtgcaggaga
     8101 cttcgccagc cagcaattca tgtccaataa aactgcagca atcattgacg gtccatggca
     8161 agcgcaaaca tacaaagaaa acaatatcaa ttatggtgta gccaaaattc ctactctaaa
     8221 taatggacag ccttatcaac cgtttggcgg aggaaaagga tgggtagtca gcaattatgc
     8281 taaaaacaaa gacctctctc aaaaatggct tgattatgta acgaatcagg aaaatcaaga
     8341 aaaattcttt gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg aaacagcaaa
     8401 ggctaaaaac gatgaattaa caacagctgt gattgaacaa tatgaaacag ctcaagcgat
     8461 gccaaacatc ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga tgttcgatgc
     8521 agcatcaggt tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa caatcagtga
     8581 agccatagaa caaaaatact gattaaaatg ataaccgtcc atttttataa aaaatggaac
     8641 aggtgcttca gataaaagtg gcaagagcga gatggaaaat tatcttgctc ttgttgccat
     8701 tatcttaaaa gcagtttgcg gtttagcaag tttagagggg gcattcagat gcagcaagat
     8761 aaaacaagca atatcaacga aaaaaaaatc acgctttgtt cacttattcc agggatgggg
     8821 cagtttgtaa atggccaagc ttttaaaggt atcgtttttt tcgccatatt cgtcctattc
     8881 attggtcaga tgcttctggg cggatttcat gcattggtag gattggttac tttgggaagt
     8941 ataccaatgg aagatcattc attatttatt ttgattgaag gcactttgca attcatcatt
     9001 acaatcattt tcttgatttt ttacggtttg aatcttttag acgcttatcg agtagcgaag
     9061 atgcgtcaag agggcaaaaa aatcaatcgg acgatgaagg aagtcataca taatataggt
     9121 gatgaaggtt ttccttatct attgacactt cccgcttatc ttctcatgat ttttacgatc
     9181 attttcccag tcctagttac attgttcact gcatttacta attatgactt caatcatatt
     9241 ccaccagcca gtttaattga ttgggtaggg gctaaaaact tttttagtat cttcttttta
     9301 agctcatatc gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg gactttatgt
     9361 gcaacaactt tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca ccagaaattt
     9421 attaaattca aacgtatttt cggtatcatt ttcttattac catgggcggt cccagctttt
     9481 attactatca tgagtttttc gaatatattc aacgatagcg taggtgccat caatactcaa
     9541 gtgattcctt tcataaacca gcttcctttt gtagatatca gtgcaatctc ttggaaaacg
     9601 gacccgaact ggacgaaagt agcaattatc ttagtgcagg gctggttagg atttccctat
     9661 atctatgtga tggttacagg aatcttacaa tcgatttcag aagacttata tgaagcagca
     9721 aaaatcgacg gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc tgctgtcttc
     9781 ctagtggcag cgccaacatt tattacacag tatacgggga atttcaataa tttctccatg
     9841 atctatctgt ttaacaatgg cggtcccgga agcgtaggcg gaggagctgg ttctactgat
     9901 attcttattt cctggatcta taaacttacg actggaactt ctccgcagta ttctatggct
     9961 tcagcaatca cgttgattat ctccgtcatc gttatctgtg tgtcattgct ggtatttaaa
    10021 aagacaaatg cgttcaatat gggggattaa caatgaaaaa atttcattcg agtgctcgat
    10081 tccaaaaacg actttctcac ttttttactt acttttttct tttaatactg tcagtcatta
    10141 tcatctatcc gcttttgatc acagcaagta ctgccttcaa gtcaggaaat attgcagctt
    10201 ttagtttaga tctttcaagt gactggactt taggaaactt tactcggcta ttcagtgaaa
    10261 cactttacgg gacttggtat aaaaatacat tgatcatttc tgtcctaaca atgatcgtac
    10321 aagtgactgt agtaactttg gcgggctata cttatagtcg ctatcgtttt atcggccgca
    10381 aatcaagtct gatgttcttt ttagtgattc agatggtacc gactatggca gcattgactg
    10441 ccttttatgt catggctttc ttattagaag cgctcgataa gtactggttc ttgacaatga
    10501 tctatatagg cggtggaatt ccaatgaata cttggctgat gaaaggatac tttgacactg
    10561 taccaataga tttagatgaa tctgctaaac ttgatggggc agggcatttc aggatttttt
    10621 tccagattgt attaccgctt gtcaaaccga tgctcgccgt tcaagcatta tgggctttta
    10681 tgggaccttt tggggatttc atgttagcta agttcctgct tcgaacacct gaaaatcaga
    10741 ctgttgctgt cggcttacaa acatttattt ctgatgtgac aaaccaaaga gtctctcttt
    10801 ttgctgctgg ggcaattttg attgcactac cgatttgtct attattcttc tttttacaaa
    10861 agaactttgt taccggcctg acatctggcg gaacaaaagg ataggagcag agtatcatgc
    10921 aaacatttcc gttaaactat ttttccgcat tacgtacgcc aactcaagtg tttgcgggac
    10981 gcaaactatt atcatggcct aaatttttct tgatatttgt atttttagtt tccttgatgg
    11041 tcatgcctgt gactcttttt tatgccaatc agatccaagc gattccatta gaacaatttc
    11101 tatccgttca ttcgctgatc gatgaacaag gaacccaaaa attttctgaa cttgaattat
    11161 ctgaaactgg gttacaagca tcgcaacaaa cgatagctgt gacccctgaa attttggtag
    11221 gtgtgtcttt atctgagaaa caacaatccg atcatggaac ttttattgat tttgagaagg
    11281 aacaatgggt gatccaacaa aaggataaat ctggtatacg ccgctatacg atgaattata
    11341 gcccatcttt ccaaccagat tctgtacgta cgcccgaaga tttccaacga tttttagaaa
    11401 gagaatttta tgccagtaat cgtccaacaa tcattttatc ttatagttta agtcttggtc
    11461 ttgtcctatt tgtcatgacg agtctcattt tattcggggc ttcctttttc ttgtggatga
    11521 caagaaaaag ccaactttca tccattcata cattcaaaga gagtgcaaat ttgatgctga
    11581 atgttatggg aataggttcc atggcagcag cagttgtagg gtttatccac tttgacttta
    11641 tcttgatgct aagtgtccaa acaacgataa ctgtcttgct gcttttatgg gtttttgtca
    11701 aaacaagatt caaagataag cgtgtcgaat gacaagttta agcgatttat gcgacaatga
    11761 atgtgaaaaa aatgaaaaat aaagtagttt ttgaaaaatt tctgaaaaaa ttaaacttct
    11821 tagttgattc ttttcttttt tagtattaac gtaatagggt agttttttta aggaggaaaa
    11881 tcatgttaga gaaaacttta tacaatcaat tattttcacg ttctttctcg ctccctgtcg
    11941 aagtaaatta ttgggacggt acaacgaaac aatacggaga tacggacact cctccccaaa
    12001 tcaaaatcaa tatccatgaa gaaatcccaa tgaaagagat tacaagtaac gcatctttgg
    12061 cactaggaga agcctatatg gatcatcgaa tcgagatcga gggaagtatc caagcattga
    12121 tcaacgatgc ttatcagcaa gcaaacagct ttttacgagg aaaagattat ctaaaatggc
    12181 tgccaaagaa agaaaaacat acgaaacaaa aaaacaaaga agatatccat gcgcattatg
    12241 acttaggtaa cgacttttat aaactttggc tagatccaac tttgacttat tcttgtgctt
    12301 atttcaatac acctgaagat acgttggaac aagcacaaat caataaagtc catcatatct
    12361 tagacaaact ctttatcaaa gaaggcgaaa cgttgcttga tatcggttgt ggctggggaa
    12421 cattgatgtt cactgctacc aaagagtata atgtcaaggc aactgggatc acactaagtg
    12481 aagaacaata cgatttgatt accaaaagaa taaaagaaga acatttagaa gataaatgcc
    12541 gtgtattatt aatggattac cgcgaactaa aaggcgaaac gtttgatcat atcacaagtg
    12601 taggtatgtt cgaacatgta ggttctgaaa atcttgaagg ttatttcaaa gtggtaaaag
    12661 atttattgaa accaaaagga acagctttga tccatggaat cagccgacaa caaggcggtg
    12721 cgacaaatgc ttggatcaat aaatatattt tccctggcgg ttatgtccct ggtgttactg
    12781 aattagttga taatattaca aaaaacgatt tgcaactgat tgatttggaa agcttacgtc
    12841 gtgactacca attgacatta gaacattgga caaagaattt tcatcaagtc caagatcaag
    12901 tcacgaaaga aatgggtgat tccttctatc gaatgtggga tctatattta caagcttgtg
    12961 cagcttcatt tgaatcaagt aatatcgatg tgattcaata tttgcttgtt caacctggaa
    13021 acaatgacat cccaatgcat cgtgcataat tgaggcaaaa gtactgttta aagactattt
    13081 aaaaagaagc taagttttat gatgctccag aagtgaactt ttttgtctaa cttctaggat
    13141 tgtctctaaa gcttagtttc tttttcttta tattttcata ataaaagttt ggttctttaa
    13201 tttttaaaca tgacagaaaa ttttttcttc gttatacttg taatagaata ctattttata
    13261 acaaagggga tcgaatcaat gaaaaaaact gttatttata ctagtttatt tgcagctttg
    13321 tttttagctg cttgtcaatc aaataccaat caaggcacat ctgggaatac gtcttccagt
    13381 agcgaacagc gctcttcaca ggtatcggac tcctcaacaa aagcaagcac atcttcgtct
    13441 agccagacaa aaagttctag tgaagcaagt actcagtcag atatcaaagt gtctgcagct
    13501 cgtgcgatcg aattgttcca agataaatac ccagatactg caattactag tttggaatta
    13561 gatagtgatt ggggatctta cttctacaag atagaaggtg tcgatgatca aaaagaatac
    13621 gaagcaagaa ttgatgctca aaatgaaaaa atggagacag agaatccaga aactctagat
    13681 cgagatgaac aaaatggtca aaagaaaaaa gaagatggtc tggatgttga taaattgatc
    13741 acgattgagg aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac ggcaacagat
    13801 tgggatttgg ataaagattt aggtactact tattgggaag tcaaagtaaa agacggcaat
    13861 caaagtacat cagtcaagat caatagccaa acaggcgaag tactgtcaac tgaacaagat
    13921 gattaagaat ttcttcggaa aacaagagtt gatttttctt ttagtcatga tacactagac
    13981 aaaataactt gtttgaagga gcgatttact tgagtttaga aacgaccttt gccaatcata
    14041 tctttgccaa tcctttgatg aatgcatctg gtgtccactg catgactacc caggaattag
    14101 atgaactggc tcattcagaa gcaggagcat ttatcactaa gagctgtacg atcaatgaac
    14161 gaaaaggaaa tcccgaacca cgctattttg atgttccttt agggagtatc aattctatgg
    14221 gacttccaaa cttaggtttt tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc
    14281 aggaaaacca aaatcaaccg ttattttttt ccattgccgg gatgagtgtc caggaaaact
    14341 tggaaatgct tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat
    14401 cttgtcccaa tgtgcctgga aaaccacaac tagcttacga ttttgaggcc acttatgaaa
    14461 cactgaaaga agtattcagc atcttttcta agccgctagg aatcaaattg cctccttatt
    14521 ttgattttgc tcattttgat cagatggctg atatcttgaa tcagtttcca ttgacctatg
    14581 tcaatgctat caacagtgtg ggcaatggtt tgtatatcga tacagaacaa gaagcagtag
    14641 tcatcaaacc aaaagaaggg ttcggcggca ttggcggaga gtatatcaag ccgactgctt
    14701 tggcgaatgt tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc attggtacag
    14761 gtgggattcg gacaggacaa gatgcatttg aacatctgct atgtggcgca agcatgttac
    14821 agattggtac cgagcttcat aaagaagggc cggaaatttt ttctagaatc ataaaagaat
    14881 taacgcagat tatgtcagaa aaaggatata cgtcaattga cgaatttaaa ggcaaattac
    14941 gcaccatttc atagcaaaga gacgaaggtc atgattgatc ttcgtctttt tttataaaaa
    15001 acactcgtct gttcattcct gtgcaagcgt actcattgta tgttagacga acttgtggga
    15061 aaataacaga ggcaaaaagg aggataatat gaatgatgca acagaattag caaaagagct
    15121 taaagaaaaa gaatttagtt ttcaagaact agttacggat atacagaaaa aaataaagaa
    15181 gcaaaatcat gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta attcccacga
    15241 gctattgatt ccccagtcag aaaaagggcc attggctggt atcccatttc ctttaaaaat
    15301 gctcggtcaa gaaaaaaagg gatggctagc tacttcagga tctcgtttat ttgaaacaca
    15361 ccgagcttct catacatcta attatgtgcg gcaagcagaa gcgataggtc tagttccttt
    15421 tggacaaacc aatgcgccag agtttggttt caaaaatatt acagacccag tcatctatgg
    15481 cccagcaaga aacccttgga accttgaatg tacaccgggc ggatcaagtg gaggagctgc
    15541 tgctgcagtt tcatcaggaa tcgttccttt agctggtgct agtgatggcg gtggttctat
    15601 tagaataccc gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc
    15661 agttggtcca tatgcttgga gaggatggca aggagctgct attgatttcg gcttgacagt
    15721 ttctatgaga gacacggagg ctttatttta tgggatgaga agtatccatt ccggtgcgcc
    15781 atatcaagta tcgccagttg agtggcaaac acatccaaga aaaaaacggt tgaagatagc
    15841 tttttgtgtt gattcaccga tccactcaca gattagtgaa gaggcagtac ttgctgtaaa
    15901 aaaagctgta gatttccttg gaaaagaagg gcatgatttg acagagattc cttacccact
    15961 agatggtcgt aaactgatca cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat
    16021 gatccgctcg attgaaacag gcatccagcg agctgtcaga aaagaagaac ttgaacccat
    16081 ttcttggact ttgttccaat atggtcagaa gctctctgcc gctacttaca tcgattctct
    16141 gcatgtatgg gatgaagcag caattgtcat ggaaaaccta catcaagaat acgatctatt
    16201 cctttcacca acgactgcat ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc
    16261 gataagagca cagatggaac acatcagtga ttgttctgaa gtagaaggtt tagacgttgt
    16321 ttcttccatg tttgaggaga gcttgaagat cacaccttat acacagttgg ctaatctgac
    16381 tggacaacca gcaatcagct tgcctactca tttgacaaaa gaaaacatgc ctctaggaat
    16441 ccaatttatg gctgccagag gaagagaaga tttgctattt caaatcggct atctatttga
    16501 agcaaatcat cagttccatt taccaaatcc atggaaatag cagaaacaag gagaagaaga
    16561 atgaagaaaa aaagattgtt tgctctaata actagtgcct tttttatttt aagcgcttgc
    16621 ggtacagctg ccgctaatca ggaagataac agctggagta atgtagaaaa aaagaaagaa
    16681 ttagtagtag cgacttctgg cacactgttt cccgcatctt attataatga tcaaaatgaa
    16741 ttagttggtt atgatgtaga tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc
    16801 tcctttaaag aatacaacgt ggatggacaa atcacttctg tttcaaaaag agaatcagat
    16861 tttgctgcga acgattttgg tatcacaaaa gaaagagaaa aaaaattctc cctttcagag
    16921 actttcaaat attcatttga cagtatgatc gtacgaaaaa aagatgattc aggaatccac
    16981 tcacttgaag atcttaaagg gaaaaaagca gctggcgaac ccaatactag ctatatgcgt
    17041 ttagcggaaa aacacggagc taaattagtc acatatgaca atgccacgaa tgatcagtat
    17101 ttaagcgatg tagcaaatgg tcgaacagat gttattctaa atgattatta tctgcagaaa
    17161 atggccgtat ctgctttacc agatatccct gtaaaaatac tggagaacgt ttatttcaat
    17221 gaaaacgaaa gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat
    17281 ggcgtattaa aagagatgaa agaagatggg acgacaaaga aaatatctga aaaatatttt
    17341 caaactgatg tttcagtcaa accgaaagaa acgatggctg agtcggtgag tgagtgatgt
    17401 actttcctct aattgatttt tccctcatgt ggacttcact tcctttcgta ctgcaaggct
    17461 tggtatacac attagggatt ggcttcgtgt cgtttctttt aggaaatctg gtagggttgc
    17521 ttttgacagt tttaggtctt ttggattggc tgcctctgaa tgtatttatt cgtttttatt
    17581 tatcgttctt tagaggcatt cccgcactag tcctgctgtt tctgttatat tttgggttac
    17641 cttatcaatt aagtgcctta acggcttcag tcatctgctt tacgattacc agcagtgcat
    17701 ttattggtga aatctatcga ggatcactag caggcgtttc ttcaggtcag tgggacgctg
    17761 gtttagcttc tggtttttct ttctttgggg tattgaaata tatcgttttg ccgcaagctt
    17821 ttcgaatctc agtaccggca ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt
    17881 tagcagcaac aatcacagtg ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga
    17941 cattcgatta tttttctctc tatattttag ttgcactgat ttattgggtc atttgtatcg
    18001 ggatcgaaaa agtgcaacag tatttagaaa aacgttctat gcatcagtca atctgaaaaa
    18061 tagaaatggt gctagcgtgg ctggcaccat ttctattttt tgtttcttgt atattctgcc
    18121 tagtatttta aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa
    18181 catgaagacc ccgctaacca tcgaaataga atcctcgtta taccaatatt gcatcgagca
    18241 aggcggcttg gttgtagaag aagtcacgat gcctgatgac caaggaattg tagatacgct
    18301 tagctgcttg acaaaatttg atggaagccg agaatggcga tgctatgagc tgaaagtgac
    18361 aaaagcagat tttcgctcta cagccaaact gtcatttgtt ggacattata attattttgt
    18421 tttaccaagt aaactatatg aagaagtaaa aactgaaatc cctacaggaa taggtgtttt
    18481 agtgtatcgt ccgtacacgg ttgaagagga gatgcctgcc tctggtacat ttctgattgc
    18541 gaaaaaagca gcaaagaaag agcttgctgt accggaagaa gctttgacaa accgcttcat
    18601 ggcttcttta ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg taaaatattt
    18661 tccaacagat caattgtaca aagaattgaa aagacgttca gaaaatcaag atccattttc
    18721 taacgaacga tattatgaac ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt
    18781 gcaagaagaa gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac aacagcagat
    18841 ccgtcggttg cctacagagc cattggagtg aaaaatatgt actatcctta tttaagagga
    18901 aaacaatttg acctattagc attaaaagaa gcactgtcgc gaggattgct ctccaataaa
    18961 atccaaccag tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta
    19021 tttcaaaaaa agcaccatcg aatcgctgtg atccaaaatc cccaagtagg acaattcaaa
    19081 ttattcgatc agcatgtgca cagttggaca gtcaaagaaa atagctctgt cgtccctgca
    19141 cagatcttaa cgccagaaaa tctcagtgaa gtactggatt cacctccagc gtttcttgtt
    19201 ttcgatggac agcactaccc aaaagataca gaggtgtgga aacagttggc aggagttgat
    19261 tcgaaatttt tgataccaga tactagtcgc tttcgcatct ggctgcccga aaacaaaatt
    19321 gtgatccgtg actcttttca aacgagaaag catgttgaaa gttatgcaga taaaacagat
    19381 gactttttta gtgatgacta tctttttttc cacgcagacg gatatattgg attttctgat
    19441 ttcacgatcg aaggtagccg ttatttcgat aaagggttcc ctagccgagc gcttgctatc
    19501 catctaacgt atattgatgc ttacggcaat atacgcgtga aacattttgt ctctgacagt
    19561 aacgactctg ctaaagacca agcattgaag tttcttgaag caggagataa aatgaaaaaa
    19621 tggatcatgc gtcaccatca tcaactcttg attacttcag gaatgattga attattggct
    19681 ctttaccagc aacaaaaatt ccctggactg ggagtgttga aaaagtggtc acttatgcat
    19741 catttggaac ttattagtca cctgctagat catccaaaag attggcttaa atattattca
    19801 aaagttcagg aactatatga agtgatccaa tgattagata atgtaaatga aaaaggaata
    19861 gaaaagagga aactggatgt atattcggca aacaaggaaa gaagaagtag aagaagtggt
    19921 agaaatcatt aatgccgcag cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa
    19981 cgggcagaaa ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag aaagctatgt
    20041 attaatagtg gaagatcaaa ttgctggaac agctgcatta gtctcaagcg tggatcccgt
    20101 ttacacaaaa atagatggta gttggaaaga atcaactggt ccttatttat cgattcatcg
    20161 tgtcgcttta gcagaaagta ctcgaggaaa aaaactagca aaaccgttct tagaacattt
    20221 gttgacgatt gcagccagca aaaatatcca agatatccgt atcgatactt atccagctaa
    20281 tgaaccgatg gaaaagacca tctattctgc tggattcact tatcaaggga tgattgaatt
    20341 tccttttgaa cacggcgaaa gaaaagcata tcaaataatc atcaattgac tataaaatca
    20401 tctgagcaaa gataaagaca ttaacactac tgaaaaaaac aatcatatga tagccaagag
    20461 gttgtaacag aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa
    20521 atttttgtgg aattttagcg tattttacga aatagctgct ttctgaaact cccttcatcg
    20581 agaggtcgtg acatagcttt tgtcacgacc tctcggctta tcctacgagt attattcgat
    20641 acatttatat caataatcct ttttttattt tatctgcagc ttcttggaac gctccatcgt
    20701 ttgacacgac taaaagtgag gggatagctt tcaaacgttt caactgtgct aaatcttcta
    20761 cataaaccgc cagtctggct gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg
    20821 attgcaatgt ttctttagag atcgtaataa agataggacg gactaccgtc ccgaaatact
    20881 catataaatg ccaaaaacca tttgctgtca aaacggcgta acaatttcct actgtcagtt
    20941 tctttttaat ttctgcttta ctggaaccat atttatgttg agcatattgg tcccactctg
    21001 caaactcatt tcccttgatc atctgattga atgtattgtc agaaacaaaa taataatcct
    21061 cttgatcttt ttccccaggt cttggtgatc gattcgtgta agtaatgatc ttttgttccg
    21121 gctgaaaaat agcttctgcg agtgaagttt ttccagaacc agaaggaccg ataaatacat
    21181 agcaataatt cattgtcatt tctcctttga tgattgaaca agtgaaaaag ctttggaaaa
    21241 tatttccaaa gcttttaaaa gtaactttgc ttttttgtat attataccag caagccagtc
    21301 acaatagcac ttaaaaagct gactaaagtc gctgttagca aaattttcaa tccgtgtttt
    21361 gcgacaagat ttcctttttc ttcatttaat cccttcatcg caccagaaat gatcccgata
    21421 gaagagaagt tggcaaatga aacgaggaag acagatataa ttgctgttgt acgctcagta
    21481 aaatggaaat taccatttgc taattctgtc atagcaacaa attcatttgt aaccagttta
    21541 gtcgccatga tactaccggc atccactgct tctttccatg ggattccgct gatgaaggca
    21601 agaggggcaa agatgtaacc taggatctct tggaaagtga tgccgaatat cccgtggaag
    21661 atagcattga tcatggcaat caaagcaaca aaaccgataa gcatagcagc aaccgtgatc
    21721 gccacatgga aaccatcaag aatgtattct cccaacattt ggaaaaatgt ctgttctttg
    21781 ttttcttcaa tgatcagctc atcttctttt tcttctaata catagggatt tacaattgaa
    21841 gccagaatat aaccgccaaa taagttcaaa acaagggcag tgatcacata tttagaatcg
    21901 atcagtgcca tataagatcc gacgatcgac atagaaactg tagacatagc cgatacactc
    21961 atggtgaaca aacgtttctc agataggaag ggtaattcct tttttatgga aataaaaact
    22021 tccgattgtc ctaaaatagc agaagctact ccattataag attccaattt gcccatcccg
    22081 ttgattttac ttaatcctag accaacagct ttcataaata atggaagaat cttgatatag
    22141 cgtagaatac cgataatagc agaaataaat acaattggca tcaatacgga aagaaagaaa
    22201 ggtgcacttc ctttatttgc tacaccggca aaaacaaagt caacaccttg cccagcaaaa
    22261 gcaagaagat gatcaaacat ttttgccaat acagaaacaa ccgctgtacc aaaagttgta
    22321 cgtaataata cgaaaccaag aacaagctgt aaaacgaaca tcacaattag tggtttcaat
    22381 ttgattgctt ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca
    22441 ataataccta ataaataact caaataatca ctcctattcc aaattccgtt ttattttacc
    22501 tgttttgcaa ataaaatact aatctttagt gaaattttca ttactttttt agagtgaaac
    22561 gcattcattt tccattagaa aataaagcga acaaaataaa aggtttaaaa agtgtaacgt
    22621 cttagtaaag tctataaaaa aacgaaacta tcgattaagc taatagtttc gttttcaaca
    22681 tttataagta aactttttct attggtaata tagattttcg gaagggtaga caggtgcgca
    22741 ggtaatatcg attcctagtt ttttcaatgt ttgttcatca tttttattga ccatcacagt
    22801 agagtgggct tgtgctccct caagttcagc tagtttatca taagcaactt gagcgattgg
    22861 attcgttact gcactgattg ataaagcgat caagacttca ttgatgctta gtgcatccaa
    22921 agaggaatga agtgtctttg atttcaatcc ttgtattgtt tcaagaacta atggtgacaa
    22981 taaaggaatg tcatctgcga tatgggctaa atgttttact gcatttaaga taactgccgc
    23041 tgacgcatcc atcaatgaac ttgtacgccc ggtaatgatg acttcatctg gtaattcgat
    23101 tgcaatcaca gcagaaggat cttttgtagg actttgcgct tgaatcttat ctgcatagtc
    23161 atgggcaggg actaccacgt ttcggtcttc aggacgtaaa ccgacttctt ccataatcag
    23221 tttgattcga ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc attctgtttg
    23281 gaaataacga cggatgattt cttgtttaga ggcttcttgg acaacggtat catccacgat
    23341 cccaaatcct acacgattca cacccatgtc agtaggagat tgataaacag attctttacc
    23401 agtaattttt tcaataatac gtttgattac aggaaatgtc tcaacatccc gattatagtt
    23461 cacagctact ttattgtagg catcaaagtg gaaggaatcg atcatattga catcttttag
    23521 atcgactgtg gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa cattccatac
    23581 tgggaaagtt tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt catgatacag
    23641 ttggttcaag gaagtagcga gctttccact tcctgggcca ggagctgtca ctacaacgat
    23701 tggtttagtt gtttcaatat actcattttt tccaaaacca tcttctgaga cgattttttc
    23761 tacgttgact ggatagtctt cgatttctga gtgtttatag accttgatgt ttcgtctttc
    23821 tagtttattg ataaagactt tcgtggcagg ctgtccacta tagcgagtga taacgacact
    23881 gttgattaat aagccataac cacgtaattc atcgattaac cgcaagatat ccatatcata
    23941 agtaatccca taatcaccac ggatcttatt acgttcaata tctccagcat agacgcaaat
    24001 cagtatttca gcttgatctt tcaatttttg cagcaatttg attttcgcat cttcttcaaa
    24061 tcctggaagt acacgtttcg catgcttgtc gtcaactagc ttcccaccaa attctaaata
    24121 taatttgtca tagtggttca ctcgttccaa aatatacgct gactgctctt caatatattt
    24181 ttctgaatca aaaccaattt ttttcactag gttccctcct taagtactaa agcatttttt
    24241 catttttttg aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg
    24301 gtttaaacca acttgtctag atagttattc attggtatga tagtttttga taaggaaggt
    24361 gctacatgaa aaaaagacag aaaaagaaaa acgcatataa acactatatc cgttcgattt
    24421 ttaccggtta tgaaagaatg cttgaagatc ccgaattgga acaattgacg tttacttatc
    24481 taaacgaaga aacgcagcta actcgtgatg agcatcagcg cattcatttc acaacaagag
    24541 atctgcctag taaataaaaa ataagataaa ggtcatcgga ttcgattctg tcctatcaga
    24601 tggaaagact agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg
    24661 gagcattgcc cgctcttttt tataacaatt ttttacagaa aatggtacta ttgaagtatt
    24721 aataagtgaa aaacagatga gtaagaggag gcaagtagta tgaaagagcc agatacaaat
    24781 gcaaattatg agccagacga actagaggag acgaatgcgg atcaggatac aggacaagaa
    24841 tcgtcttctt tttacgatat caaagaaaaa gcaagcatga atctttccca attattagaa
    24901 gacttacagg cttttgaaaa agctgtccgc gaagaagata tccctgagat ctaccgttta
    24961 tacaatggcc gattgaatga agaattaaaa agaagctcaa atgaaaatca cgagatcgat
    25021 caattgctga tgcgtaagat ccatgatcgt tttttacagg aatttccttt tatggaacaa
    25081 gtagaaaaca tctcgccaac gatgagctat tacaaaatcg gcacttatta ccatgatcgg
    25141 cccacggtcg gtattgatgc aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa
    25201 gagtgggaga aatattccca accggagtca acggactatg acaaaaaaat caatgagcta
    25261 gacgcaaagg tgatcactgc tcagacagaa attgaacgtt tagatgaaca aataaaagaa
    25321 atcaatcgac aaatgactga tttgaacgac aataaaggat tcttgaatcg taaaaaaatc
    25381 gaggaagaaa ttcaagaatt agagaagaag aagcaagtac tatctaatga aaaattaggc
    25441 tggcttcctt atattgagac gcctgaaaca atccagcagc aaaaagaagc attgaagcaa
    25501 gaagcaagag cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga
    25561 cgctatttcg gtagcaaaga gggattcggt caagcaatcc atgaattctt gatgaattat
    25621 cttggcaatg agaatcctaa taatcaatct aatgaaggag gttccgagta tgaatgaaca
    25681 cgatgaagca gaatggacaa aaaatgaaga acaaacagaa gatcatgaag aaacgaacac
    25741 ttcattggaa gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc ctatgctaga
    25801 ggaaacaaaa atacacgaaa aagaaaaagt aactccgacg aatacgaata ctgcagaaaa
    25861 cacccatgct tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag agcaagatca
    25921 tcaagatatc gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag
    25981 aaaagatttg atcgaatcag attttccaca attattagaa tcatatgatc gttttatatt
    26041 gaatgaagaa ccaatctcca cgatcggaaa gacaagcctg atcgaatact tcacgtatga
    26101 attactgaaa ccgttgaatg atattggtct ggaacggtca aattcagctt atgacacatg
    26161 gaaaacaaga catttttcga tcaattatac gttgaatgga caaaacgaat tagtcttttc
    26221 ctttgtatta ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt
    26281 atcaccagag acaatggaaa tcaatgtcca agatgaccgg gtgctttctt tgatccgaca
    26341 ctggtcagtg gatcgagtat tctctgcagg gcagatcacg atattcaatc acaaattgaa
    26401 tcaattatta gcccatgcaa gaacacttgg ttttaccgtt aatcagacat tactagacaa
    26461 tacgaagcca cttcgtttga atcttcagag cgaatttgaa ttgacgaatg aagtattgga
    26521 tgatattttt atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac
    26581 tacttatcaa gtacgattag ataaaggaca gtctttaacg ataacaacaa acgaaaaaga
    26641 tcaaacagtc ttgtcgatct catctggaga ctacccacga tctgtcattg atttctttat
    26701 caattacgaa ttcttggtcc cattaatggt gagaaagtcc tgattggata agttggatta
    26761 ccagatgtca ttcagataat tctgttccaa aggacttgcg gcagttatcc agaataattg
    26821 tcacaagtct tttctgtatg tactaaatgg acgtgaataa cttgctgatg aaggagggat
    26881 ttcattagga atgtccacaa ataaaatagc acagcttagc ttattgtctg ctgcttgtgt
    26941 agcaggtagg atcgcttttc aatttattcc gaatttccag ccaatgactg ctattttttt
    27001 atttatagta ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc
    27061 gatcagcagt ttttattttg gtgttggacc atgggtcatc ggccaatgga tcagctatac
    27121 tgttgttctg agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg
    27181 gttaaaggga gtctgttttt ttctggcggg catggtttac ggaataggta tgaccgtttt
    27241 tgatacgctg ttatatcgtt tgcctcaacc ttggatctat taccttcaag gggtgtcgtt
    27301 tgatttgatg cacagtatag gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa
    27361 acgtttttac aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa
    27421 tgagtgtcag tatactcatt ttgacaagtt gcagtacaac ctatacacaa tcggcttcca
    27481 accaaaagga ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc
    27541 aaaacttttc ggaaaaagaa gtgttcttca aaaaaggcga tgatttactg agtattttga
    27601 aacgaaattt tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa gggcactcac
    27661 aggatgagaa aaatcaactc tattggatgt ttactgtgga cgggaaaagt gctactactg
    27721 gtgccaaaga gatcaaattg aatgatggac aatcggtcat tttcaatttg tctaaactat
    27781 aagtttcata aaaaactgtt aaagggcgaa tattgccata attcagtgag ttcagttgat
    27841 gaaagaagga aaataacatg gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg
    27901 ctctaggggc taatattcta gttgctatca gcaaatttgt cggctatgca atcagtggca
    27961 gtgccgccat gctaaacgaa agcatccaca gtatcgttga ttgcagcaat cagattttgc
    28021 tgttattcgg tgataaacaa gccaataaag gacagagcga gttgcatcaa tttggtgaag
    28081 gacgagcaaa atactttttc agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg
    28141 cacttggggt gatggaagct tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc
    28201 cgatcattgt tatagcaata ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg
    28261 tggcaatgaa agaaatcaaa gaattgaata ctgaaaaact ttccacgatg cgctttttgc
    28321 gcgaaagtcg acacagtgaa atactgatta tttttacgga agacttgtgt gctgtaatcg
    28381 gtttggtctt ggctttagca ggaacaatat tgacttatgc aaccgggatt gctgcatttg
    28441 atgcgatcag cggattgctg atcggtttct tgctgatggg agccgccatt ttcctagcaa
    28501 aagaatttta tagtctgatc atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca
    28561 agttagcttt tgaacgtcct gagatcagtc ggctgatcga tataaaaaca gttcatttaa
    28621 gtccaacgga gattttagtt gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag
    28681 cttactcggt catcaatgat attgaaaaaa atatgcgtca gtctcttcct gacaagaaga
    28741 tgtatatata cattgaaaca gataaatatg acccaaatta ttctcgtaaa taaaacgttt
    28801 attcatgaat tgaagcaaaa atgagaaaaa tataatcttg tttttaaata aaacggctac
    28861 aatttagcga atttatgtta caatgagtcc gctgtttatt tgttgtaatt aaaggagata
    28921 ttttaatgaa caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca gtgatgccac
    28981 tttgtataag ctatatacca gtaggattag cttgcggagt attgttgcaa aaagttggat
    29041 ttgatcctct cttatcagga ctgttatcga ttctggtctt ttccggtggc gcgcagtttt
    29101 tagttgcatc tatgcttacg acgcaagcat catttgcaac gacattattg atggttttct
    29161 ttttagaatt acgctataca ttattaggag caagtcttgc cggttttatg aaaaaagaaa
    29221 agcggacgtt tttggctgtc ttttctcaat cactgaatga tgaaaactat gcggtgaact
    29281 acttaaagtt ttcaactgac ccttcctgga ataaaagaaa ggctttatat gtcaattggt
    29341 tctcaatgac tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc tcggtgattc
    29401 gagtagatgc tgaccttgtc cattttgctt tgacggccat gttcattttc atgtttatca
    29461 tgcagatgaa aagcgcacta ctgatcttta caggtttatt ctcaggcgta ttaggggtcg
    29521 tattcatggt attattccaa aatactttcg gattgattgc agcaacagtc attgcttcat
    29581 ttgccggatt tatgctggag aaggcattca aaaaagaaaa agctaagaaa ctaagagacc
    29641 gcggtaaaga tgtcaatgag ccgctcttca acttcccgga acaggaggcc aatcaccatg
    29701 actaaactat atttgctact tttagttgct tgtctgttca tcgtggcata tatcccgcgc
    29761 ttgttcccga tgctttattt tactcatcga aaagtgccat cctggttcag tgactggatg
    29821 aaatatgtac cggtggcatt atttgcggcg ttagctttta aagatgtttt cattactcat
    29881 gaacatctgg atattgcttg gaatatcaaa atagctgcaa tgattctagt agcaggcgtg
    29941 gcttacaaaa cacgttcgat ggcgttgtca gttttgacag gacttgcgtc tgtctttttg
    30001 ctgtctatgc tttaaactgt tttattttat taaaaacaga gattgcgaca taagttgttc
    30061 aaactgatct atccgaactg catttgcaac agtcaattct ggatgcaagt ttattcgtag
    30121 ggataaaaaa gattagtgtt ggcttatgat gcaagctctt ttttatttta ggaattagag
    30181 agggatttgt ccttctatga ttttttgcta taatagggaa gatgtttatt ttatgaaaga
    30241 atagttgagg gaatttaaat gaataaaaaa ttgatgcaag gaacattttg gctaaccttt
    30301 gctaatcttc tatgtaaagt tttaggggta gtctatttga ttccctggct aagtatgatg
    30361 gggaacaatc aagatggtat gcttgctacg acattgtata atgttggcta tcttccttat
    30421 ggattgttcc tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa aaaagtagct
    30481 gtagccacaa aagaaggaga taacgctgct tgtcggctga tttttagaag tacgatcaat
    30541 atcatgtttg tcatcggtat cgcctctgct gcactgatgt atttatttgc tcctttacta
    30601 gccggaatca gtccgattgc taacgttaat aatgggatac tagctatcag aagtttatgc
    30661 ccatctttga ttgctatccc aattcttagt gcaatgagag gatatttcca aggaaaaaat
    30721 gatctgcggc cttatggaac ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc
    30781 ttggctggta cttattactt gcgcgtattg actgacggaa ccatattaga agctgtattg
    30841 atcagtacag tcgcttcttt cttcggtggg ttagctgcga ttatccatat gtatatcgtc
    30901 ggacgcagaa aagacttctt cgaacttaaa gattttttga tttccagccg ttatttcgaa
    30961 agagaaaacc gatctgcctc tgtctctatc attcgggaaa cactaccgtt tatttttgtc
    31021 ggctcagtca ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac
    31081 tttttccgtc cagagatcgc agatcagcaa ctggaattct tattcagccg tgcttctgtc
    31141 aatccaaata aattgacatt gatcctgatc tccatggtag gaacagtagc tataagcagc
    31201 ttgccaatat taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga
    31261 gatagttttt cgattgcgtt attgattctt cttccttcat tgactggaat gtctttatta
    31321 gctggaccac tatacacatt gttttttggc tatgatcctg aaagtgtcgg atatttccaa
    31381 atggctttgc tagcttcttt gtttttctct ttattcacga tcctatctac gatgcttcaa
    31441 tctttgaatc accatcgttt tgcgatccga ttaactgtag aagcaattat cctgaaagtc
    31501 atcttccaag taatcggatt aggattagtt ggcggatacg ggatgagctt atcaaatggc
    31561 ttagcattcg gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga ataccgaata
    31621 gcacctttag caaagatcag caattttttc ttaaaaactt tccgctccac cctgattatg
    31681 cttgcagtat gttttgccgt tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa
    31741 agctatgcaa tgatttattg tgtggctgta ggaagtattg gcggcttagt atttttattt
    31801 tctcaattcg gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc
    31861 aaagaatagg ttaactaatc gttttttgct gtcacttgaa aaacggcaac tcgtatgatt
    31921 acgcttgtcg tttttctatt ttccaatttt agtttcatat ctaatcttac ttatgatata
    31981 gttataaggt ctggaaaaat tctttgaaac gaggaaatta aacacatgaa acttgcagta
    32041 gttacagata gttcggcata tctgccggaa cgtatcaaga accatcccga cttattcgtt
    32101 attccaatcc ctttgattat ggatgggaaa atttataacg aaggaatcga tatcgaagcg
    32161 gatgaatatt atggactttt aaaaaacagt aaagattttc ctactacttc acaacctgtc
    32221 gtaggggaag ttttgggttt atatgaggaa ttgaaagaga aagggtatga cacgatttta
    32281 agtatccatc tttcttcagg gatttctggc tttgtcaaca cattatttgc gatgaaagat
    32341 gacatcagcg gagtaacggt cattccttat gactctaaga tcactagtat gccgatggga
    32401 cacatggtag aagctgcctt agacttaaat gatgaaggtc agtcccttga tgaaatactt
    32461 gcacatatcg atcgaatccg agataatacc tacgcttatc tgattgtaga tgatttgaac
    32521 aatttagtac gaggaggacg tttgacaaat ggtgcagcgc tgattggcag cttattaaaa
    32581 atcaaaccga tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa aatccgttca
    32641 agtaaaaaag catttgctcg agctgaagaa attattggta gaagaaataa cgaaatacaa
    32701 ccgccagtca aactttacgt gatacatgca aataatttag aagtagcgat tgcggagaaa
    32761 gagaaattgc agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc
    32821 attggtacac atttaggtga aaaagccatc ggcattgcca tttcagcgca ataattcctt
    32881 ttattcaaaa aacataccaa aaatattcca acatcatctt ggactttcta catgttacta
    32941 atgagcgtgt aaaagaacga gatggtgttt ttatttctat caatgaagct ctttttcttt
    33001 gcaatcctca cttcaatcct ctataatgtc gcatatagtt tagtaaatga gtaaactact
    33061 aatttactta tttactaaaa ataaaaggat gaggagttaa aataatggaa ataccaacaa
    33121 atttaaaaca taaaccagta atcgttgtag aagactatga taaagttgat ggaagaaatg
    33181 cattaaatac ggatgcaaaa gggctttcac tgggccttgc tcagtggaat gatcgtggaa
    33241 aagtcgatat ctcaggcaag gtatggcgac atactggaga aaaatggtca cgacagtcgg
    33301 aagaattacc aatcacacgt attttagatt tagcgatctt agctgcacaa ggaagtttat
    33361 acttccaaga tgcttatcgt tatgaaaaat tttatgatcc agaaaatcca gtcgtagaca
    33421 tcattggatt acaagggaac cgaatgacgg tagaagtaga taccaaaaat ccaaagatcg
    33481 atgaagacat cattttgtat tacgatacac tacaaaaaga cggagaactg attggggaac
    33541 gattccgtat tttaaaacgt cttttagatg agctaggata ttaatagaca gagatgatca
    33601 aaaggggaag agaagcatgg agaatttctc actaacagaa aaccgaacct tttcacggct
    33661 agaaaagcaa atgatctgga agaaaccgaa aagccatcgg acaagtgcag aagaatggcg
    33721 gatcgcctcc gaaatcaaag ggaattggca agatcctgaa atgaaaatca gtaacgtact
    33781 tttggaagga gatgccgggt cggggaagac acaattagcc aaagcactat ctgctgatct
    33841 gcaacttccc tatacaaaag tgacctgttt tgcagatatg gacaaatcag atgttttcgg
    33901 agccttatta cctgtagtag attctaataa ggaacaagac caagaattac tagaagctat
    33961 ttaccaaaca gatacacttg aagctgtact gacactgatc cagaaccatt acggcattga
    34021 tcctttttct gctaaagaaa aattagcaga gctagttcaa aggatcgaag ctgattatcc
    34081 agatgcaatc cattacagct attacccttc tgaaatcgtt cgagcgattg aaaaaggata
    34141 tttattagag attcaagagc cgactgtcat ccgagatgcc tctgttcttg tggcacttaa
    34201 ctcagcgctc gaaccaaatg ggatgttgaa tttaccgaca ggaatcattc gtcgccatcc
    34261 agattgcgtc attgtgatca ctacaaatcg aaattaccaa ggaaatcgcc cattaaatga
    34321 atctttaaga gatcggatgc aacatgcgga aaaaatggat cttcctgcct tagaagtcat
    34381 ggcagaacgt gcgatggcga aaactatgat ccaaaaacaa gaacttctct taaagatggc
    34441 agagattatc cgtttgttgg atgaaactgc aaaagctaac gccatcaaag gtgtagcagg
    34501 aatgcgttct tatttttatt gggcaaatac ctataagcaa ggacaagatt tactccagtc
    34561 gatttatcca aaagttttgt ataaattatc cactgattca gatgaattgg caattcttga
    34621 acaagctttg tctgacagtg gattgctact ggaacttcag gacatattga gaaaagaaaa
    34681 atggggtcat cctcaatctg agcatacaaa aggaagaacc attagcgccg aagaagcaaa
    34741 tgaacgaaac atcactgaaa atgctgaaga aattctgcgc ttagatgaaa agaaatcgtt
    34801 gtctgctgaa gaaccaaatg agggagaaga gaaacaagaa gaaacaatag aaaaaaacga
    34861 gattcctcct caaatgcaaa aagaacgcag tagcgatatc gaaggaaaaa atcaaaaaga
    34921 acaacaaaca ggagatgatt ctcaagaaat gtctgcttct gacatggaag cacatgataa
    34981 ggaaatcaag aagcaactga ataaagaagc gcgacaaatc atgaaggaaa cgattcatga
    35041 aaaagaaggt ctcatcgttc accgaccagc tttccaagga aaaacgccgg aagcacgtga
    35101 gctacacatg caagtaatgc caatagtaga atcacttagc cggcaaattt tagagttact
    35161 ggataatgaa caaacagaga cgtaccaaaa aggaaaatac gaaggtcagc ggttcaatgc
    35221 aagcaaagta gcatatggtg atttgagaaa ttttgacaaa aagaacccgc cacatgagca
    35281 accttcttta gcggtagctg tacggatcga tgaatctggg tcaatgatac gggatgatcg
    35341 gatcgaagcg gcaaaaaaag cagcgattgc cattgccgaa ttttctaaga aagtcgatat
    35401 cccattgttg atttacggag atacagcaga ccgttcgtca agagaaaaga cttctttgtt
    35461 ttcttataaa gaatttgaag acggatttca atggttaaat gaaaaattag tcactatgaa
    35521 accaaggcaa aacaatcgtg atggtgctgc acttcgttta gcagcagaaa aattaaacag
    35581 acaatccgca acaaccaaac tgttgcttaa tattagtgac ggtcagccaa aagcgttgcc
    35641 ggattataca ggcaaaaaag caaaagaaga tatccagtct gttttgaaag aatatgaacg
    35701 tcaaggaatc ctatttgtct ccgcagctat tggccaagac aaagaagaaa tcaagtcgat
    35761 ctacggtgaa agtcgttttg tagatattac tgacttgaat gaatttccca agcaaatgat
    35821 tcaactgatt gcccgctatc tatgataaaa taaagctcaa cgaacgcact tagcagattc
    35881 gttgagcttt tcgtttctag ttaccggtaa caaggatact tctaaaatag aatcggttat
    35941 aatcaagagg gaccgttaaa ggagtgaaac gatatgatac aagaacaaaa atggtggaaa
    36001 gaagcagtag gctatcaaat ctatcctgct agtttcaaag atagcaatgg cgatggtcaa
    36061 ggagacatca atgggatccg tgaaaaactt aattatttga aagatcttgg tatcggcttt
    36121 atctggatca atccgatcta tcaatctcca tttgtcgata atggttatga cgtaagtgac
    36181 tatcaagata tagaagagag attcggaacg atggaagatt ttgatttatt gctaaaagaa
    36241 gcacatgaat tagggatcaa agtgattatg gatcttgtca tcaaccatag ttcggatcag
    36301 catcaatggt ttgaagaatc gaaaaaatct aaagaaagcc cgtatcgcga ttattacatt
    36361 tgggtcgacg gggtagatgg caaagaaccg aataattgga catcgatttt cggcggttct
    36421 gcttgggagt attctcatga aactggacag tattatctgc atgtttttgc aaaagaacaa
    36481 ccagacttaa attgggaaag cgagaaattg aaagaagaac tattcaatat gatccgatgg
    36541 tggttggata aagggataga tggatttcgt ttagatgcga tttctcatgt aaaaaaagat
    36601 gaatattcag taaaagccac agagaaccct ttctcaccct ttcaaaatgt ctcaggaatc
    36661 gaagaacatt tgaccgattt gaaacatgtt tttgaagagt acgatatcat gactgtcgga
    36721 gaagcaagtg gtgtcactgc agaagaagga ccacaatggg tagggaaaga cggttatttc
    36781 gatatgatat tcgaatttga tcatatccat atctggcagc aagaaaaaga agggcaatta
    36841 gatgtcctta aattgaaact tgcattaagc gcttggcaaa cttcattgga tggtatcgga
    36901 tggaacgctt tatatatgga aaaccatgat gttcctcgtg ccgtttcagt gtttggagat
    36961 actcgtcccg atttttgggc tatgtctgca aaagcaatcg ctatgatgta tttctttttg
    37021 caaggtacgc catttattta tcaaggacag gaaatcggaa tgacgaatat gccctttgaa
    37081 tcgatcgatc aagttgatgc agtagattcg aaaagacttt ataaaaggct gcttgcggaa
    37141 ggtaagacaa gagaagaagc attggatatc attcgtgaaa caacgagaga caacagcagg
    37201 acacccatgc aatggacttc ggaacaatat gctggttttt ctacacatga accttggctg
    37261 atcactaatc caaataccaa aacgatcaat gtagaacaac aagaatatga accagagtcg
    37321 gtcctgcaat tctataagaa catgatccgc attagacaaa ccaataaagg tctgatatac
    37381 ggttcttata aagaatatct tcatgaacat ccacaacttt atgtctatga aagatattta
    37441 gaagatgaac aatatcttat tatggtaaac ttaacagaat cacttgctga ctatgaactt
    37501 ccaaaagaag cagatcagtc ctggacatta ctcttgtcaa attcctctag cggagaattt
    37561 gaagctaaag gcatactagc accttatgaa gctcggttat ataaaacaaa caagtaaaac
    37621 ttcctcgtta aagttcatta gttggacttt gcgaggaagt ttttcagatt gctctgtcaa
    37681 ttactcattt caataattcg ctctaggacc accgatatat ttaggacgag agcgaagggc
    37741 tgtcacgtga gcttgtccaa gggttttcaa acgaggtctg acaggtactt ggacatgttt
    37801 gatatgcata ccgattgatg tgtcaccgat atcgataccg gcttgtccaa ctacgtgttc
    37861 cacttcgaca ggatcattga actggtcaaa agcagcaaca gaacaagcac ctccagcatg
    37921 tagtgcagga acaacagaga caatttcgaa attttttgct tctgccactg ttcgttctac
    37981 tactaatgct cggttcaaat gttcacaacc ttggacagct agcgcaattt ctttaggatc
    38041 caacaactct tttaacgtac ggatgatcca ttgaccaact tccgcactgg aattttttcc
    38101 aatatgtcct ccaacgactt cgcttgtgct gcatcctaaa acaaaaagat cacctttttt
    38161 caagtgtgcc tcagccaata tgtcattaac tatttcagtc aattggtctt ttaattcttt
    38221 ttctgacaac ttcattttct aaaaccttct ttttcaatta atattgcttc cgtgcttgaa
    38281 tacgttgaag acttgcagtt ataggaaagg caagtcccat tccaatcaga ttttggaaga
    38341 tgtttcctgg tatagaagcc actccagccg gccaatcata taaaatccaa ccggcgatac
    38401 catagccgat gatcataact aagcttccta ataaaagccc taatccccac atacgagtag
    38461 aagatttgct gaaagaacca gcaacatatc cttgtaaccc gtggatcact aatgaaaaaa
    38521 ctgcccactg cggatatcct gaaatcaaat cgatcaatag accactggaa gcaccaacaa
    38581 ctaatccacc cgttggacca taaagaaaag cagcggtata gatcccggcc tcgcataatg
    38641 tcacaaatcc attcgtagcc ggtacaggga tcaaaattgt taatgaaagg gcaactgtca
    38701 acgcaatcag catagcatag atcgttaatt gcttagtttt catttctcat tcctcctttt
    38761 tgaataccgc cagaccagac acttccggta tgatcgttca tcagcacgcc attagaaata
    38821 caatgggcaa caaatttttt actttctgct actgctttgg taaaatctgc ccctaacaca
    38881 agattcgctg ttattgcaga agaaaaggca caaccggccc cgtttactgt tgtagtgtcg
    38941 atttttttat gagaaaatac ctcataatga tcaattgaat agagaagatc cacagcttca
    39001 tctccagcca tccttgtgcc gcctttgatc acaatgtttt ttgttcctga agcagtgagt
    39061 tctttagcga gatggatcat ttctgttaac gaagagggaa gagcttgacc agaaagtaaa
    39121 gcagcttctg ccaaattagg cgtaatcatc gtaacaagtg gaaaaagttc ttcgatgagc
    39181 tgcgaaatat acaattgatt gatcacagct tcagtttctt tgaatgccaa aacagggtct
    39241 aaaatgattg ggatattttg ttttgttttt atgaagtctc gaacaaccat gattgcttca
    39301 cttgaattca ataacccaat tttgatacca tctaatgaag tcattttatc aatcgaaact
    39361 aactgtcgtt taagcaattc caaagatata gattccagca taaatcgctc attaatatct
    39421 agtgccccta tagaagtcaa ggcgcttagt ccgaatagac caaaattctc aaaagttttt
    39481 aaatctgttt ggattcctcc accagcaaat ggatcagaac cgccaattgt caagattctt
    39541 ttcataacat tttcctccat gaataagagt ataacgaaat tttcaaaaaa gaaaaacagc
    39601 caattggcat atttttatct catccaattt aaaaaaacaa aaagaaaaga aagaccaaaa
    39661 tggatctttc tttccttaaa tagttaggac ttttgtgatt ttaattcttt ctcgatttga
    39721 agccgttgca ctttcattgt agctgttcgc gggattgcat caaaatcacg gatgataggg
    39781 acgttcaagc gaggaagttc atggacttgc tcccaccatc gattccaatc catttcttta
    39841 tcaggagcta aagcaataat cggttgtgga gaatgatttt tatcccttac aatcactact
    39901 tctgccaaaa aatctaacga atctaataaa aagtcttcaa tagctaatgt actattgatc
    39961 gtttcgatca aatccacttg ccgatctttc aaaaataggt ggccatgctt atccataaaa
    40021 ccgtaatctc cgctatccca ccaatcgcca taaacatttt cctggaaccg tgcatcttcc
    40081 ttgtaataag tcaaagcccg tcctttagac agtaattgta tatgaccatc tgtgttttca
    40141 ggtaggacat tcccaactga atctgtaatc cgagcgcttg tcatgtcttc cagtccaacg
    40201 cccatatctc ttgcatcact tgttttcaaa ctctctagtg tatgagcctt aagaatcatt
    40261 ggtccgcatt cactttggcc atatacttga agaaataccg catcctgtgc catcgacgtt
    40321 cgcaaaaatg tcttcattgt ctggttgttt atagcatcaa aagtggagtg atagtatcgg
    40381 atacccgaaa aagcttctgg atgctcctta gcagtaaaac gccactgaac aaagttattc
    40441 ggatgggtct ctagtgcaat tggccggtga gcttccaaca tatgaacaac ttttgaactt
    40501 tgtgcgtttg ctaaaggcat cataggaaat cccatggaca tcaaagaaga gataccaata
    40561 ttgaatctgg aatgcactgg agagatgtga aaagcgatta atttcttttc cgaaattttt
    40621 gtgaaaactg ttttttgcca tttcgtccgc catcccatag aatgagcaga atgacaaata
    40681 agtttaggga taccagttgt accagaagta tgggtcatat aagaaatttc atttttgtct
    40741 aaaaatgatt gagaaacaga caaagcaggt tgttccatta aataaagaat aggaa
//
LOCUS       DASJTC010000013        39891 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.013, whole genome shotgun sequence.
ACCESSION   DASJTC010000013 DASJTC010000000
VERSION     DASJTC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39891
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.013"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            126..995
                     /locus_tag="U4B19_000643"
     CDS             126..995
                     /locus_tag="U4B19_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEN2076404.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            1160..2431
                     /locus_tag="U4B19_000644"
     CDS             1160..2431
                     /locus_tag="U4B19_000644"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN2076405.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            2428..3723
                     /gene="rho"
                     /locus_tag="U4B19_000645"
     CDS             2428..3723
                     /gene="rho"
                     /locus_tag="U4B19_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEN2076406.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            3822..4085
                     /locus_tag="U4B19_000646"
     CDS             3822..4085
                     /locus_tag="U4B19_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEN2076407.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            4530..4979
                     /locus_tag="U4B19_000647"
     CDS             4530..4979
                     /locus_tag="U4B19_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2076408.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            5036..6454
                     /locus_tag="U4B19_000648"
     CDS             5036..6454
                     /locus_tag="U4B19_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2076409.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            6454..7314
                     /locus_tag="U4B19_000649"
     CDS             6454..7314
                     /locus_tag="U4B19_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEN2076410.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            7488..8261
                     /locus_tag="U4B19_000650"
     CDS             7488..8261
                     /locus_tag="U4B19_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2076411.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            8408..8773
                     /locus_tag="U4B19_000651"
     CDS             8408..8773
                     /locus_tag="U4B19_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEN2076412.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8921..10129)
                     /locus_tag="U4B19_000652"
     CDS             complement(8921..10129)
                     /locus_tag="U4B19_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2076413.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(10148..10426)
                     /locus_tag="U4B19_000653"
     CDS             complement(10148..10426)
                     /locus_tag="U4B19_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076414.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            10580..11275
                     /locus_tag="U4B19_000654"
     CDS             10580..11275
                     /locus_tag="U4B19_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2076415.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(11717..12514)
                     /locus_tag="U4B19_000655"
     CDS             complement(11717..12514)
                     /locus_tag="U4B19_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEN2076416.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(12511..13695)
                     /locus_tag="U4B19_000656"
     CDS             complement(12511..13695)
                     /locus_tag="U4B19_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN2076417.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(13720..14967)
                     /locus_tag="U4B19_000657"
     CDS             complement(13720..14967)
                     /locus_tag="U4B19_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN2076418.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            15182..16927
                     /gene="dnaX"
                     /locus_tag="U4B19_000658"
     CDS             15182..16927
                     /gene="dnaX"
                     /locus_tag="U4B19_000658"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEN2076419.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16947..17261
                     /locus_tag="U4B19_000659"
     CDS             16947..17261
                     /locus_tag="U4B19_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEN2076420.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            17286..17882
                     /gene="recR"
                     /locus_tag="U4B19_000660"
     CDS             17286..17882
                     /gene="recR"
                     /locus_tag="U4B19_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEN2076421.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17946..18590
                     /locus_tag="U4B19_000661"
     CDS             17946..18590
                     /locus_tag="U4B19_000661"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEN2076422.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            18605..18934
                     /locus_tag="U4B19_000662"
     CDS             18605..18934
                     /locus_tag="U4B19_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEN2076423.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18948..19886
                     /gene="holB"
                     /locus_tag="U4B19_000663"
     CDS             18948..19886
                     /gene="holB"
                     /locus_tag="U4B19_000663"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEN2076424.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19922..20746
                     /locus_tag="U4B19_000664"
     CDS             19922..20746
                     /locus_tag="U4B19_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEN2076425.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            20739..21086
                     /locus_tag="U4B19_000665"
     CDS             20739..21086
                     /locus_tag="U4B19_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEN2076426.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            21155..22027
                     /gene="rsmI"
                     /locus_tag="U4B19_000666"
     CDS             21155..22027
                     /gene="rsmI"
                     /locus_tag="U4B19_000666"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEN2076427.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            22136..23257
                     /gene="dinB"
                     /locus_tag="U4B19_000667"
     CDS             22136..23257
                     /gene="dinB"
                     /locus_tag="U4B19_000667"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEN2076428.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(23311..23913)
                     /gene="nrdG"
                     /locus_tag="U4B19_000668"
     CDS             complement(23311..23913)
                     /gene="nrdG"
                     /locus_tag="U4B19_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEN2076429.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(24228..26417)
                     /gene="nrdD"
                     /locus_tag="U4B19_000669"
     CDS             complement(24228..26417)
                     /gene="nrdD"
                     /locus_tag="U4B19_000669"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEN2076430.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            26716..27510
                     /locus_tag="U4B19_000670"
     CDS             26716..27510
                     /locus_tag="U4B19_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2076431.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            27500..29542
                     /locus_tag="U4B19_000671"
     CDS             27500..29542
                     /locus_tag="U4B19_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2076432.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            29823..29972
                     /locus_tag="U4B19_000672"
     CDS             29823..29972
                     /locus_tag="U4B19_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEN2076433.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            29983..31497
                     /gene="dltA"
                     /locus_tag="U4B19_000673"
     CDS             29983..31497
                     /gene="dltA"
                     /locus_tag="U4B19_000673"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEN2076434.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            31494..32699
                     /gene="dltB"
                     /locus_tag="U4B19_000674"
     CDS             31494..32699
                     /gene="dltB"
                     /locus_tag="U4B19_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEN2076435.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            32744..32977
                     /gene="dltC"
                     /locus_tag="U4B19_000675"
     CDS             32744..32977
                     /gene="dltC"
                     /locus_tag="U4B19_000675"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEN2076436.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            32980..34248
                     /gene="dltD"
                     /locus_tag="U4B19_000676"
     CDS             32980..34248
                     /gene="dltD"
                     /locus_tag="U4B19_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEN2076437.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            34389..35471
                     /locus_tag="U4B19_000677"
     CDS             34389..35471
                     /locus_tag="U4B19_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEN2076438.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            35471..36274
                     /locus_tag="U4B19_000678"
     CDS             35471..36274
                     /locus_tag="U4B19_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEN2076439.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            36344..37084
                     /locus_tag="U4B19_000679"
     CDS             36344..37084
                     /locus_tag="U4B19_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2076440.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            37095..38099
                     /locus_tag="U4B19_000680"
     CDS             37095..38099
                     /locus_tag="U4B19_000680"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEN2076441.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(38144..39841)
                     /locus_tag="U4B19_000681"
     CDS             complement(38144..39841)
                     /locus_tag="U4B19_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076442.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        12789 a         7021 c         8280 g        11801 t
ORIGIN      
        1 ttcttgaaaa ttttcatttt cattgatttt agtagaaaac taccgaaatg tttggtaaaa
       61 tagggtcgtt ggttaagaat tgtcttaaca aatttagttt tacaaaactt aggaggaatt
      121 ttattatgcc agtagtatca ggagctgaat ttttaaaagc tgctcgtaaa ggcggttacg
      181 ctgtcggcgg attcaacaca aacaacttgg aatggactca agcaatcctt gaagcggctg
      241 aagcaaaaca agcaccagta cttatccaaa catcaatggg tgcagctaaa tacatgggtg
      301 gttacaaaat ctgtaaagat ttaatcactg atttagttga agcaatgaac atcactgttc
      361 cagttgctat ccacttagac catggtgatt acgaagctgc tttggaatgt atcgaattag
      421 gctatacttc agtaatgttc gatggttcac atctaccatt tgaagaaaac ttgaaattag
      481 ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc agttgaatgt gaagttggtt
      541 caatcggcgg agaagaagac ggaatcatcg gtaacggcga attagctgat attgaagaat
      601 gcaaacaaat ggttgctaca ggtatcgact acttagcttg cggtatcggt aacatccatg
      661 gtcaatatcc agaaaactgg aaaggattag cttttgacca cttacaagca attgctgatg
      721 ctgtaggtcc agacatgcct ttagtattac atggcggatc aggtattcct caagatcaaa
      781 tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt taacactgaa ttccaattat
      841 catttgctgc agctactcgt aaatatatcg aagctggtaa agataaagaa ggtaaaggat
      901 tcgaccctcg taaattatta gctccaggta aagcagcaat cgttgaagat gctaaatctc
      961 atatcgactg gttcggttca gctaacaaag cgtaagcatc cacataacag atacgatata
     1021 taggaatgga gaacgacttt tgaagtcgct ctccattctt ttttacttaa aacggcagac
     1081 catgctcgtt tctttttttt atctatgata aaatacaagc atatccatga aaaaagatta
     1141 ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa ggaaaccgac ctttagctgg
     1201 tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca ttgattccag ctgcaatctt
     1261 agctgattcc ccagtgatct tggaaggtgt gccggacata caagatgttc attcattaat
     1321 tgaaatttta gagatcatgg gcgcaactgt ccatttttcc aacaacatac tagaaatcga
     1381 tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa atcaatagtt tgcgtgcttc
     1441 ttattatttt atgggaacac tgcttggtaa atttggcgaa gcggtagtag gcctgccagg
     1501 tggctgttat ttaggcccta gaccgattga cctacacgtc aaaggatttg aagctctagg
     1561 tgctgaagtg acaaatgaac atggtgccat gtatttacgg acagaaaata aaggattgcg
     1621 tggcaatcgg attttcatgg acgtggtttc agtcggagca acgatcaatg tgatgttagc
     1681 tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct gcacgagaac cggaaatcat
     1741 agatgtggca actttactga ataatatggg ggcaaaagtt cgaggagcgg gtacagatgt
     1801 gatccgtatc gaaggagtcg aaacattaca tggctgtcgg catttcatga ttcctgaccg
     1861 catcgaagca ggaacctatc tagcattagc agctgcagta ggaaatggta tcaaagtgaa
     1921 aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa ttgcaggaaa ttggtgtcca
     1981 tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg aaagaattaa aacccgcaaa
     2041 catcatgacc tatccttatc caggatttgc cacagatctg cagcagccac ttactgcgtt
     2101 actattgatg actacaggca cttcagaaat catcgatacg atctatgcga aacgtgtgaa
     2161 ccatgtgccg gaactagcac gaatgggcgc tgatattacc gtggaaggaa atatgatcat
     2221 cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg gcttctgatc ttcgggcagg
     2281 tgcttgttta gtgattgctg gactattggc agaagggaca acgacgattt acaatgtaga
     2341 ttatatctta cgcggttatg atcatattat cgagaaacta acagccttag gggcatcaat
     2401 tgaaatgata gaggaagtgg gagctgaatg agcgattatt tgacaatggc agaactagag
     2461 aacaaaacgt taaaagaagt ttatagttat gccaaagaat ttaaaattcc ttattacagt
     2521 aagatgaata aaaaggaatt atctctagct gtgattcgtg cacaagcaga gaaacaagga
     2581 ttttattata tggaaggaat attggatatc gttggtcaag atggttacgg ttttttacgg
     2641 ccaatcaatt atggaccaag tgcagaagat atctatattt ctgcctcaca aatcagacga
     2701 ttcagtttga gaaatgggga caaagtggca ggaaaagccc gtccacctaa agaatcggaa
     2761 cgttattatg gtttgatgca cgtggaaagc gttaatggaa aagatccgga agaagcaaaa
     2821 gaacgtccgc attttccagc cttaacacct ctttatccgc aaagacagct tcaactggaa
     2881 acaacaccgc aaagactatc tacccgaatg atcgatatct tttctccaat cggttttggt
     2941 cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa cttctgtatt aaaagaaatc
     3001 gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa tcgttctact gattgatgaa
     3061 cgaccggaag aagtaacgga tctagaacga agcgtgaaag gcgacgtggt atcttctaca
     3121 tttgatctgc agccgcaaaa ccatactcga gtttctgaat tagtattgga acgcgcaatg
     3181 cgtttagtcg aagataaacg agatgtcgtt atcttgatgg atagtattac tagacttgct
     3241 cgggcctata acttagtcgt tccaccaagc ggacgtacat tgagtggcgg gattgaccca
     3301 gccgcattgt ataaacctaa gaaatttttc ggggcagcaa gaaacatcga agaaggtggc
     3361 agtttgacga ttttagcgac cgctttagtc gatacaggaa gccgtatgga tgacgtgatc
     3421 tatgaagaat tcaaaggaac tggaaatcta gaattacaac tttcacgtga attatctgaa
     3481 cgtcggatct tcccagccat cgatatcaaa aaatcaggta cccgaaaaga agaattattg
     3541 atgccaagtg aagaattaga agaaatttgg aaacttcgaa aaaatatgac tggagattca
     3601 ttagaatata cggaacaact gatccgcttt ttgcgtaaaa caaaaaacaa tcagcaattt
     3661 ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc cgaccagaaa tctgaaaaga
     3721 taattgcaac cttgactgaa acatgttaag atgttaatgt tgtaaatcga caaatcaact
     3781 ctggttcagc aaatggagca gggcaaagga gcgaaaaaat catgaaagaa aacatccatc
     3841 cagattacca cccagtagta ttcatggatt caacaactgg tttcaaattc ttgtcaggtt
     3901 caacaaaaac ttcacaagaa acagttgaat gggaagacgg taatacatac ccagtcatcc
     3961 gtgtcgaagt aacttctgac tcacatccat tctacactgg acgtcaaaaa ttcacacaag
     4021 cagacggacg tgtggaccgt ttcaacaaaa aatacggtct caaagacgca aacgctgcgg
     4081 aataataaaa acgctgataa ctcaacgttt agcagactat ctgataggga tagtctgttt
     4141 ttttatgttt tttgccaatt taaaacttcg tttgatgctg aaaaagtcgt aggatcataa
     4201 tgcacaaaac aatgaaaaac tagtatatag ttttaattat ccacttgata aaaacacctt
     4261 atattaacga aatgaagagg ggttatttcg aagtaaaaaa ccatttacct aaaattagaa
     4321 gctaagcttt cctttcaagg aaggcttttt gttttgttat agacttgcga ttgtttgtga
     4381 gctagatcgt tcattttaat gacttataca cgcggatgat gggtcttttt cctctgttca
     4441 ttcataatat tgaacggtac agctagcgat gctatactga cctcagttcc aagaaaacgt
     4501 taacattttt tgtgggggag aggtttttta tgaggtttga tcaattgatc gcagaagaac
     4561 tgattgtttt cgatccatcg atagattcaa aaggatcatt atttgagcga ataggggatg
     4621 tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt cgttaaggca ctattcaaac
     4681 gagaggagga gatctccaca ggaatagaag atggatttgg gattccccat gcaaaaagca
     4741 aatatgtgac aaaaccagca ctgtgttttt ttcatacagc caaattaacg gactatagcg
     4801 ggatagatga aatgccaatt gaatatattt ttgccatcgt agttcctgaa aaatcagcag
     4861 atatgcatct agagattctc agcacactct ccagacgatt gatgaatgca gattttagaa
     4921 agggaataaa agaagccact acgccagcag atatcatcag acagattagt caagaatgaa
     4981 aatagtagag tctttgtccg agtatcgata gtgaagaaaa aggaggaaaa taatcatgaa
     5041 aatcgttggt gtaactgctt gcccgactgg aattgctcat acttatatgt cggcagaaaa
     5101 attagaagta acagcaagaa atctaaacca tcaagcaaaa ttcgaaacac aaggtgtaaa
     5161 aacagaaaat cagttatctg aacaagaaat caaagaggca gatgcaatca ttcttgcagt
     5221 agataaagaa atcgaattgg accgatttgc cgggaaaaaa gtaaagcgtg tctctacaag
     5281 ccgagcaatc aaagaaccgc aagtagtaat agacgaagca ttgagagata ttggcgtatt
     5341 cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa aagccgacaa tctataatca
     5401 tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc gcaggcggaa tcatcatcgc
     5461 actgagtttt gcctttggga ttacagcggc tgatcccaac tcagcagact ataatgtgtt
     5521 agcagctgca ttttcaagga tcggaggaga tacagcgttt gccatgatgg ttcctgcgtt
     5581 aggcgcgggg attgctacat cgattgctgg aaaagcaggt tttgccccag gaattgttgc
     5641 cggattactg gcctctacag gtggttcagg ttttcttggc ggaatgattg gtggcgtgct
     5701 agcagggtat atctgtgatt ttctggcaaa taaaattagc gtaaaaaaag aattttctgc
     5761 tatttatcag ctgattgtcg tccccttttt atcgatctta attattgggc ttttgatggt
     5821 atttgtgatt gaacaaccga tcacatgggt gttggatgct ttgacaaatt ggctgaatag
     5881 cttgggaaat acatccgggc tattgttcgg attacttatc gggatcatga tggcagctga
     5941 tatgggtggg ccgatcaata aatcgacttc gactttctcg atcggtttga tgtcagctgg
     6001 agtaaatgca ccgattgctg cttgtatggc tgcaggaatg acacctcccc taggtattgc
     6061 tttagccact ttattattca aacataaatt taccaaagaa gaacggactt caggtcaatc
     6121 atgctggatt ttaggcgctt cgtatattac agaaggtgct ataccgtttg cggtatctga
     6181 cccgatccgc gtgataccta gtttgatgac aggttcagca gtcgctgcag ggatttccat
     6241 ggcagtagga gttacttcaa tggcacctca tggagggatt tgggtgatgc taattccaaa
     6301 tgttatgaac aatttaccta tgtatgtctt ggcaattgca tcaggtacag tcactacagc
     6361 ctttatgctt ggactattga aaaaaacagt gaaagagaaa aaacaagaca agtggacgaa
     6421 aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat atacaatgaa agagttattg
     6481 aaagtggcta aagagaatca atttgcagta ccagccttca atatttgcag ctatgatatg
     6541 atgaaagcaa tcatcgaaga agcagagaga ttagaagcac cagtaatctt agaaattcat
     6601 cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg cagtaagagc ctatgcttat
     6661 acaagtaaac tgccgatcgt tatccacttg gatcatggcg gtacgataga agatgtctta
     6721 cgggcgattc gaaacggcta ttcttccgtg atgatcgatg cgtctttgaa atcattcgac
     6781 gacaatgtac agattacgaa aaaagtggtt gcgttagctc atgcggtaaa tgtctcagtg
     6841 gaggctgagc ttggaacgat tgggaataat ggttctgctg aaggaggtgc tgaaacaatc
     6901 atttatacag atcctgatca agcgaagatt tttgtgaaag aaacaggaat cgatacactt
     6961 gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa acagaactcc aaaattaaat
     7021 atcgacttac tgaaagaatt gaaccaacaa atcgatcttc catttgtttt acatggcggt
     7081 tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat acggagttgc caaagtgaat
     7141 ttgagctcag atcttaaaag cgtctttttc aatgctttga gagaaatatt gagtgaaaat
     7201 ccatcgatgt acgagccgaa tgcaatttat ccagcagcta ataaaaaggt acaagaagtg
     7261 gtcaaacata aaatggaaat tttgaacaca gtaggcaagg ctagtttata ctaaaaataa
     7321 aaatgtgtgg tcaagagggt atgacaatag tcttgtcata ccctcttaac cacacacgat
     7381 gttcagaagt agttttttcg aaaaatttct tcttatttct cgaagccaat cacttctgtc
     7441 acaacctctt ttttcctgtt actatataaa gtaaatgggg tgattttatg ttgaatgaag
     7501 tggagaatag acagcaggag attgtgaatt atctaaaaaa caaccaattt gcaagagtca
     7561 ccgatttgat cgagttagta aattatagcg aagccacgat taaacgtgac cttgtttttc
     7621 tggaaaaaag tggattgatc cgtagagttc gcggtggcgc catgcttatt gacaatcgaa
     7681 aaattgacgt gccttacctg atgaagatca ccaaattaga agaagaaaca gaaaaacgta
     7741 agattgcaga tacagcttct tctttgattc gagatgatat ggtcttattc ttggattcca
     7801 gtacaacctc tttacactta gtcagaaacc tagcaagatt tgatggtttg cagatcatta
     7861 cgaatggcat cgtcacagct gctatgttga gtgaatatac aacggcaaaa gtatctgtat
     7921 tgggaggggc aattgttccg aaacgagcaa caatcaacgg ttcaaaagcc tataacgatg
     7981 cattgtcata ttatgcggat ttagcgataa tgagttgtcg cgggctagat ttcagccaag
     8041 gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa agcttttaga aaacaatcag
     8101 accggcttgc cgttttagtt acacagcaaa aaattgcaca taaattcatg catcaagcca
     8161 ttgcttttca tgaaatcgat tatctggtga caagtaaaga actgacaaaa gaacagtatg
     8221 cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata aattattacg tgaatgttct
     8281 aagtggcttc ctcattttta gataggacct tatctttgct cgcttctctc agccgatggt
     8341 ataataatga aaaggttatc ttgttagata atcttacttt tttatccagg agaaaggagt
     8401 gcagaaaatg aaacatgtga aggcgttggt agtgaaggca atcatgatct gggcgattct
     8461 gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg gattcaacga ttgttggggt
     8521 aattattgta gtcatgatct atgtgctagg ggatctgttg atcttacgta aagtagggaa
     8581 catcgcagcg acgatcgcag atgctggttc agctgcagtt atcttgtggc tctatctata
     8641 ttttatgaat gatacagtcg atatttggat gaaagtattg attcctgctc tattgatcgg
     8701 tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa ggcatcgtac cagatgaacg
     8761 gaaaatggaa taaatcaaca gttaaactat tcttatcgat aaatgaaagc tttaaaaata
     8821 aatagatgtt taattaagaa taatcataaa ctatttttac tcaaaaaacc gctcagatta
     8881 ttttcagatc tgaacggttt tttttatcta ttcatcaaga tcaaatttca tttttcaatg
     8941 attcaatcaa ttcgccctct gcttcatgac tatgttttcg gccattaaca agatagagtg
     9001 ctgtacatat caatggaatg actccggcaa tcacatacaa tcctctaaat gaagtaagag
     9061 attttagcat ccccatcaaa taaggaccaa cgcctaagcc taggtctaaa ccaataaagt
     9121 aggtagataa agcaatactg attcgatgtt cgcggacgat ttttaaacaa acagcctgtc
     9181 catttgacat aaacgtacca taacctaggc cgactaatgc tccagagata agcaatgtca
     9241 cactattcgt cgttacactc agtaaaaata atcctgctgt taagaataaa tagctaggat
     9301 acatcacgta ttgttctcct ttgacatcga aaatccttcc agtcaatgga cgtgtaaatg
     9361 tgatgaccaa agcatatacg acgaagaaaa atgacccagc agcaactaag tggattactt
     9421 ttgtataaga agataaaaat gataaaacac ttgaataaga aagacccatc aaaaaggcaa
     9481 ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt gatattccat gagcgaagag
     9541 atattttttg ttcttcagat aatggaatat tttttacagg aaagacaagg caggcaatcg
     9601 taacaaagaa gaccagtaca attgaaaacc agataatgaa acgaaaatca gtcatattca
     9661 gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa actagtactt aatccataat
     9721 aattgatgcc ttcccctttt ttagatgcag ggatatatgc agtaacgata gcatttgtag
     9781 ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg aactaaatac aatgccccga
     9841 tagttggcgt ataaagatat gctaatgttg aaaggagata gaagatcgcg ccgcctctta
     9901 aggtaaactt ccgaccaaat agttctaact gtttccccat gaataagcgt gccagcaatg
     9961 ttccgatgat atagatccca gaagcaaaac ctgcttggct caagctggcg tgtaaggtgt
    10021 cttgcgcaat gactgccatg atgaccatca acagataata aatcaggtag acaataaaat
    10081 tgatcaatgt gattccaata aaatatttat taaataattt cttttccatg tatgttatac
    10141 attcccctta agctgaatat gcttttaatt tttgtatttg aacttctaca tcgtgataat
    10201 tggatggata gcgatcaaca agttggcgaa taaataaatc tcttgtagac ataaacagat
    10261 ttaaaaattc ttcgcattcg tttgttggat tctcatccac tgctttgatc aattcgatcg
    10321 agttttgata cgcatcattt aaaaagacta aagaagcttt catatcaaat cttgtttgat
    10381 atgctgtttc taatcgatta atgattgtgt tcatccagta attcatgttt tttccctcta
    10441 ttcttaaaaa attttacttc tgcagtataa cgaataaaaa aaaggaaaaa aatcgcataa
    10501 gtgatggaaa aaggtactta acaaatgtta atatcattag taaaatacct ttggacttta
    10561 aaaagtttta aagtactgta tgatcttttt agatgaaagt ttggatgggg gagcatcatt
    10621 ggatagtaga gtcttaaagg aatatttgga taaaaatgat tttccgatta tacgcaaagg
    10681 atatcataaa tatttgacat tcgaagggat ggaagattcc catacgtata tcttgaaaga
    10741 gggaacggta aaagcaagtg ttatttcaaa tgatgggaga gaatttaacc ttcgctatat
    10801 caaagatctt gaaatcgtct ctcttttaaa agacgaatat tcacaattca tcgattcgcc
    10861 gtataatatt cgagttgaat cagaatatgc agagttttat cgaatcaatc gtgtgcgctt
    10921 ttggcaaaat atcaaccaag acccaaagct acaacactat gtaaaagagt attaccgcta
    10981 tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta ttaaacggaa agctgggagc
    11041 tgtttgtacg caactttacg aattatatga tttattcggt gtgcggacag aagaagggat
    11101 gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa ttttgtggca tctcaactgc
    11161 tagcagtgta aacagaattt taaaacaact gaaaaaagat ggtattatca ccgttaaaga
    11221 ccatcatatc gttattttga atatggactt gttagaagac aatattatct tttgatgaac
    11281 aaagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg gaaaaatagt
    11341 tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta
    11401 ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag
    11461 cttttcatgt ttgatgaata ttgtgacttg tgaccgagga gttggtagga gaacaccgtt
    11521 taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat aagaagagcc aagtaaaaac
    11581 agtctcttat gtttttaact tggctggact tatcaccgta gatgtaagct tttgaacgcc
    11641 gtttatttgc aacaaaaggg gctgtgacac gatttttgtc acagcccttt ttttgcgaac
    11701 attcatttag caatttttag aaacttctgc cgccaccgcc aaaagtcccg ccaccgctag
    11761 aatgggtagt acttccgcca ccgccgcttc caggaggtgg gcttttgggg attcttcttg
    11821 tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt taatttgata ctggattttt
    11881 ctcggaatgg atatttgtag atgcctgatt tcaattgata acgcgaaaca gtcacaaaat
    11941 aaaagacaag actaaggata agagccaaaa caacagcaat gatgatttct actgtcgtca
    12001 aaaccttata gcgtgtaatt ttgccagttt cttcgtctac acgataatgt cctcctggaa
    12061 ctccgtcttc cacatatgtt gctgatttag acaggtaagc ttttgcagca ttgtaatagg
    12121 cagcatttgt catctgatca taaacatcat ctaagatcga atcgattctg ctatcagtca
    12181 aataatcgat catattacca gaagtcgaaa tgtagatttc acgctgtccc atatctaata
    12241 gcaatactga gccattttga tcatttccga ctgcgtctct taagtaatta tcagcaaagt
    12301 ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa gactcgtcct ttgatttttt
    12361 cattcagctt ttctgcctgt tgttccaaag attggatttg atctgttgtg aataacccag
    12421 cctcatcatt taccgtagct gtatctgcat gagtagccac cggccagcca acacttacta
    12481 gcaaaagaaa gaataaaaaa aattttttca tcatatcaag taacctccga tcagaaagag
    12541 gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc aattttttct ggctgatcgg
    12601 caggacaccg ctgacctttc ctgtttgtcc attcattgcg tagtagtaga cttttttact
    12661 tgagtcattg cttcgataag taacaagcca taccggcagt aatacgtaat tatttacttc
    12721 gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt gtatatccat ttgcggtatc
    12781 ttttaacagc ttctctgaat agtcttttaa ttcagattga atctgtgatt tgattgcctc
    12841 atattcgata tctctttttt ctgcttggaa cccggctaaa tactggcttt taaaatcaat
    12901 tgctttatct aacgggaaag gttgcacgcc ttcgaccatc ttttgttgcg tattttttga
    12961 taaggcattt tttactagtt ctttaaaaga aagcttgcct gctcttgaaa cagcaaattg
    13021 cttcgtttct gtgtattcga tgtcacctat ccgccatatg cggataactg taccattcgc
    13081 ctgcatggca ccatctactt cagcatctgt agcccaataa gggaaataca cacctgtcaa
    13141 cttatcgatt tgatccttat tgaaaaaagc tttgggaaca aaccattttt tcttggtcca
    13201 agcgagaaat ctgttgattg cttcttcttt ctcgacagca aaaggaagta ctttatttgg
    13261 caagaattct ccgcttaaac gtcccgatag tacgacggga ttatggcagt agtaacaata
    13321 agtcgcagca gttgtcgcat cagtcacgat ctctgcacca cagttgggac aaagaaaaag
    13381 atctatcgct gcggtattat ctgcagattc gtccgctgta tctgaaaaac cgcctgcctc
    13441 gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc tgttcttggg ctgtaaaagt
    13501 aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg ccagccatac gcgcttcttt
    13561 ttgcttttgc tcatattggc tgacttcttc ttctgtatag atattcaggc agtattcgca
    13621 atgaaatttt tggtctttgg gatcaaaagt caacggaccg ccgcaattag gacatttatg
    13681 tgtaagaaca tccatcagct gcacaccttt ctaaataaat taattaacgg ggattccttt
    13741 aaacggttta ccacagtttg gacagaattt agggatgccg ttggaaagat ccacgatctc
    13801 attgcattca ctgcatcgca tttgtagctt aggctgaacg ttttctacag gtttggcagc
    13861 tccgcagttg gaacagaatt ttccggaatt ttctgtcccg caaactggac atgtccaagt
    13921 atccgcattt ccttgtgcag tttgttgatt ttgtttttct gcttgttgtt tgatttgttc
    13981 ttggttattt tgagctgctt gagaaagata agaaccattt gcattcattc ccatacctac
    14041 acccataaag ccggtcattg ctcctgcttc attttttccg gctgcttcca taccacgtgc
    14101 aatggaacct tgcacgtagc cttcacgtac gctaggatca cctagcatcg ccccttcgtt
    14161 acgcatattg atcagtttca cagaatcatc ggtgtaagaa atgctggcta cagccacaga
    14221 aacaatctcc atcccacgta attcacgcca agaatcatcc aatgcagtat ccatgtattt
    14281 gcttaattcc aaactcttag atggtacata agaaatccgt tgaccatctg cagacatctg
    14341 attgattgct gattgtagcg ctgttaggaa ttcagctaaa tactgctcat tgatatcatt
    14401 gatttccact tgtgttttgt ttttagggat tgcgtttgta taaaataaaa tcgggtcaac
    14461 gacatgaata gaataagtgc catgtgcccg taagaataat tctgcgttat agaaattgtc
    14521 aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc tttatttctt ggagattgat
    14581 ataaaaaact tgttgtttct gcggtgtgac gccgccaaat ttgaatcggt caaaagtttc
    14641 agcaattgct tctttcagtg ttccattgaa catggaagga gctgcgtcat ttttaatggt
    14701 gtaataacct tcctcagcgg tataatcgat gatttttcca ccatcgacca aaagcatcat
    14761 catgtttgga tagacatgga cgactgttcc gtctgttaaa acatcttctg ttcctttacg
    14821 attggagcca cgtttgtcat ctttgcggac tttgacacct tttgtcatga ccgttgtatc
    14881 actcatattg tctggttcga tgacttctag ccattgatct gccagaccgc cgccaaccat
    14941 acttgttgct gctttgataa gacccataaa aaattcctcc tctgattcag ctagtgccaa
    15001 acaattattg tttatttatt ataccatagg aagatgtgaa acttctcgga ctagagacgg
    15061 aattgaaatg gaaaattgcg tattgtttaa atacctgaaa aagccgagaa ggatagtagt
    15121 ttttttacct aatatcacgt ataataagag atagtgaatt ttttaaaaaa gggggatcct
    15181 tatggcatat caagcacttt atcgagtctg gcgttcgcag cgtttcgaag acatcgttgg
    15241 acaaaaagct gtgacacaga cactaaaaaa tgctattgtt tcccacaaaa cctcacatgc
    15301 ctacttgttt acaggaccca gaggaaccgg aaagacgagt gcagcaaaga ttttcgcaaa
    15361 agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc aacgaatgcg aaatgtgtcg
    15421 atcaattact gctggtacgc aagaagatgt gatcgaaatc gatgctgcca gcaataacgg
    15481 agtcgaagaa atccgattta tccgtgatcg ggcaaattat gcgcctacaa aggcagcgta
    15541 caaagtgtac attattgatg aggtacatat gctttcaaca ggagctttca atgccttgtt
    15601 gaaaacattg gaagagccaa aagaaagtgt catattcatc ttagcaacga ccgaacctca
    15661 caaaatacca gctacgatca tttcacggac tcagcgattt gattttaaac ggatcaatac
    15721 ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa tcgactatca gctatgaacc
    15781 agctgcattg aatgtgattg cccgttctgc agaaggcggg atgcgggatg cattgagtat
    15841 tcttgatcag gcaatctcgt ttagtgaagg aacaattact ttagatgatg ccatgcaagt
    15901 gacaggtagc ttaactgacg aaatgatgga cagttacttg agtgcttgcg tgaaaaaaga
    15961 agtaccagaa gcattggaaa cattgaacca aattctcgca gccggcaaag aatcacgtcg
    16021 ctttctagag gatatgttac aatactgccg tgacttgctg atgtatcagc aagcacctaa
    16081 cctagttgag gaacaatcga gtacgatcag aagtacattt acagaattgg cacaaactgt
    16141 ttcagcggat cagctgtttt caatgataaa gattttgagc gatactcaaa atgaaattcg
    16201 ttttaccaat agtgccgcta tttatttgga agtcgctaca gtcaaacttg cacagctacc
    16261 gtctgcttct gttccttcag cagatgtagc atttgcagat gatcagatag taaaacagct
    16321 acaacaagag attaaggaac taagaaacga aatcaaggaa ctacgcagta atacaggaat
    16381 tgagaataca aaagtacagc cggtacagca aacaaatcat aaaaaacaga caagtaccta
    16441 tcgtgtaccg acggaacgag tatataaagt tctgcgggaa gcgacaagac agcatctgaa
    16501 ccaagtaaaa gaagtatggg aagacttgct gatgagtcta tctgtcactc agcgagcaat
    16561 gcttaaagca agcgaaccag ttgcagctgg acctacagga ctagtgattg cgtttgatta
    16621 tgagattgtt tgtcaaagag cagcaaatga cgaagaattg cagctttcgg tacacaacaa
    16681 cttaagccga atgatcaaag attatgcgcc tgatcctgtt tatattacaa gagaaagctg
    16741 gccagaactt cgaagaaatt atttgacgca ggcacaagaa gagggaacag aaaactcttt
    16801 tgcttctgaa gactcagaag gagaaatcga gttgcttcct caagaacaag aagtaccaga
    16861 agaaacactt gtagtaaaag cagaggaact gtttggctcc gaagctgtta cagtggtaga
    16921 tgattaataa aaaggagtag ataattatga tgcgtggaat gggcaatatg caaggcatga
    16981 tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc acaagagctg ctaaataaaa
    17041 cagagtttac aggtaaagcc accaatgaat tggtcaaagc ggtttttaca ggcgatcgtc
    17101 gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc tgatgatatc gagatgcttc
    17161 aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca aattgatgtg gaaactgaga
    17221 aaaaattagg gaaatacgca aaaggtttgc caggatttta gttttaaact gaaggagtaa
    17281 tgaatatgca atatccagaa ccaattgcga aactgatcga cagctacatg aaacttccag
    17341 gaatcggtca gaaaacagcg acaagactcg ccttttatac gatcgatatg aaagatgaag
    17401 tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg cgatttgcat ttttgtagta
    17461 tctgcggaaa catcacagaa gaagatccct gcgaaatatg tagaaatcct tcacgtgatc
    17521 gaagcgttat cctagtggtc gaagagccga aagatgtaat ggcaatggaa aaaatgcgag
    17581 aatatcacgg actttatcat gttctccatg gtgtgttatc accgatggaa gggacaggtc
    17641 cagaagacat caatatttct tctctgttgc agcggctgca tgatgcagat gtaaaagaag
    17701 tgattatcgc aacaaatgca acaacagagg gagaagcaac agctatgtat ctctcacgtc
    17761 tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca tggtctgtct gtaggctctg
    17821 atatcgagta tgcagatgaa gttactttat tgaaagctgt cgaaggaaga cgtgagttat
    17881 aaatttctaa ttaagtgttt tttcagatac aatataaaag cagcacaaga gatgggggca
    17941 acaatgtgaa cggactattt attacaatcg aggggcctga tggtgcagga aaaaccagca
    18001 tcttgaatga actttaccct cttttgaaag aagtagcaaa aacagaaatc attcaaacaa
    18061 gagaaccagg aggcattccg attgctgaag agatccgagc agtgatcctt gatccaaaaa
    18121 atgatcggat ggatgaacgt acggaagcac tgctttatgc agcagcaaga agacaacatc
    18181 tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat cgttttatgc gatagatttg
    18241 tcgacagttc tcttgcttat caaggtgcag gccgacgcat cggcgttacc gagattgctc
    18301 ggttgaatga gtttgctaca gaaggtacta cgccggattt taccctttat cttgacgtag
    18361 attcagacac cggattaaga agaatcaaga aaaacagaca aaatcaaatc gaccgtttag
    18421 attcggaagg tttggaattc caccagcgtg tacgtcatgc ttacttaaag ctggcagaag
    18481 agaatccgga aagaatccac aaagttgatg caagaaaaag ctttgaagaa gtgctgcaaa
    18541 caagttatca tacaattatt gaacagtatc cgcaattttt tgaaaactag gaaggtgaac
    18601 gtttatgaaa attattttag cgattatcca agacaaagac agcaaccgtt tatcaaatga
    18661 attgattgat gcaaatatcc gtgccacaaa actatcatcg acaggcggct tccttaaggc
    18721 tggaaacagt acgtttatcg tgggtattga agatgaccgt gtagaagaag cattggaaat
    18781 catcaaaaaa acgtgtgaat caagaaagca atttgtatct acacctgtta cactggatat
    18841 ttcgatggat gggggcgtcc cttatccagt agaagtagaa gtaggcggtg ccacagtctt
    18901 cgtcttacct gttgaaggat tccatcaatt ttaggaggag ctgagctatg aatcaagaaa
    18961 taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct tcaacggagt tttgagcatg
    19021 ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg aacaggaaaa catgaagtag
    19081 gtatatggct tgcacagcac ctgttctgta cccaaatgaa agacaatctg ccttgtggaa
    19141 aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc agatgttttg gttattgaac
    19201 cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt gcagacggaa ttcagtcgca
    19261 gcggttacga atcaagaaaa aaagtttttt tgatcaaaga agctgaaaaa atgaatagca
    19321 gtgcggcaaa cagtttgttg aaattcttgg aagagccccc tggagatttt ttagcggtgt
    19381 tagaaacaga tgcagtagga agaattctcc ctactattca atctcgttgt caagttctgc
    19441 attttcaaga actttcgaaa gaagcactga tccatcgatt gcaagaagag cagattccat
    19501 tggaaaaagc aaaacttttg gctttcttaa caaacagttt aggaaaagca gttgaaatct
    19561 ctcaagatga atggtttaat gatgctaagg acttgattta tcaatggttc gtttacttgc
    19621 aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa attggtaaaa tcatttaaag
    19681 aaaaaacaca acagtttaca ggattatctg tcttgatgtt ttattatcag gaagcacagc
    19741 gtcaagcagt agcaaacggc gaatatggca aagtgaaaca gataaaccag ataattgaac
    19801 ggatattgat cgcagagcag aaactacgca gcaatgtcag ttttcaggct gttgcggaac
    19861 agttcgtttt gcatacgatt ttttagcttg ccgcaaagga tgatgatcaa ggaggatgga
    19921 aatggtagaa gtagtaggtg tccgatatag agatgcaggt catatctatt attttgcacc
    19981 aggagactct gaatatgttt atagtgacaa ggtattagtc gaatctcagc aatccttaca
    20041 tattgctact gttgtccttc ctaaaaaaga gattgatccc aatgaccttc ctgaagctat
    20101 ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa aaagacaaaa aaaacaaaaa
    20161 agatgcttat gatgcattga aaatcgcaaa agaaaaagtg cagttgcgtg agttggaaat
    20221 gaaaatggtc aaagccgaat acacatttga tcgaaataaa ttgatttttt ctttcacagc
    20281 ggacgggcga attgacttta gagaattggt aaaagatctg gccgctgtat ttcgtactcg
    20341 tattgagctt cgacaaattg gtgtccgcga tgaagcaaag ataataggcg gaattggtcc
    20401 atgtgggcgt ccattatgct gttcttcttt tttaggcgat ttcgtgcctg tatcaatcaa
    20461 aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa atttccggtt tatgcggacg
    20521 cttgatgtgc tgtctgcaat atgagagcga agaatacgag acagcgaaaa aagaactccc
    20581 tgactttgga aaagtaattg ctacacctga tggtaaaggg aaagtcgtag ggttgaatct
    20641 gcttagccgt atcgttagta ttcggctgac tggacgggaa aatcctgtcg aatatgaatg
    20701 ggatgaattg aaagaattta tccaagtagg tgagatgaat ggataagaga tcattatacg
    20761 acggattgaa tcaattagaa tcagagttga gaaatacatt aacagaactt gttgaaatga
    20821 agcatgcttt gcatgagatc gtagaaaaaa atacaacttt ggagatggaa aatcaacgtc
    20881 tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc tccagctgaa aatgtgaagc
    20941 aggaactttc taagtctcgt atgaatcttg aaaaaattta tgaagaagga ttccatgtct
    21001 gctacgattt atatggatca agaagggaca atgacgagcc ttgtgcgttt tgtctagaag
    21061 tgatctatcg cgaaagcggt cgatgaccaa taacaagagg agactggagc ggagaaatac
    21121 tgctccagtc tttttattaa ggagattgtg acagatgtat aatcaaaaaa gtttcaaggg
    21181 aaatccggta tatggggatt tatatcttgt accgacgcca attggaaatt tagaagatat
    21241 gagctacaga agtgttcgta tgctgcaaga atccgatctt atcgcaagtg aggatacgcg
    21301 taatacacaa aagttattga atcattttga aatccataca ccccaaaaaa gtttacatga
    21361 acataattat aaagaacgtg tccctcaatt gattgctttg ttaaaagaag gaaaaacgat
    21421 tgctcaagtc agtgacgcag gaatgccttc catcagcgat cctggacatg agctagtttt
    21481 agcctgcata aaagaaggga tttctgtgat ctctatccca ggacctacag ctgggatgac
    21541 ggctttgatt gcttcagggc tagtgcctca accattttta ttttatggct tccttccacg
    21601 taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa gaacagacag ttacgctgat
    21661 tttttatgag tcaccttatc ggatcactgc tacattgacg aatttcttag aaatttttgg
    21721 aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc catgaagaat accttagagg
    21781 aagtacggaa gagctactcg cttatatcga agaacatcca gtaaagggtg aatgttgtct
    21841 gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa gttgatgaag agttgggaac
    21901 gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa aaaccaaatg ctgctatcaa
    21961 agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc taccgtcagt accatgaatt
    22021 agactgagat agtataagtt gtctataatg aaagactaat gaggaaatca gctactaact
    22081 tttcaaatct cttgaacgcg aacgaatgtt cttgtataat gaataagagg tgaggatgat
    22141 ggagctgcaa tttcctttac ataatgatac atcaagaaaa atcattcata ttgatatgga
    22201 tgcttttttc gcttcggttg aagaacgaga tcatcctgag tttcgcggac atccattagt
    22261 aattgccaga caccccagtg acactggtgg gcgtggagta gtgactacag caaattatga
    22321 agcaagaaaa ttcggaattc attcagcaat gagtgcacaa aaagcgtatg agctttgtcc
    22381 gcaagccatc tttaagcctg gcgaccatca taaatacagc gatatttcca aacaagtacg
    22441 ggaagtattc tatcgttata cagatattgt ggaaccagtt agtatcgatg aagcgtatct
    22501 tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc aaaatcgccc gaatgatcca
    22561 agcagatatt tgggagagtg tccagctaac atgttctgcg ggagtaagtt acaataagtt
    22621 tttggccaaa ttagcatcag actatcaaaa acctagagga atcacggtag ttgcccctca
    22681 agacgctgtg ccatttttaa aagctttgcc aattgaaaaa tttcatgggg tagggaagaa
    22741 aactgtacct agaatgcatg agctagggat ttatacagga aaagacctat atgaatgcac
    22801 agaaatgatg ttgattcgaa attttggaaa aatgggctat tctctttatc gtaaagtcag
    22861 aggaatccat gattctccag taaacgttac aagagagcga aaatctgtgg gaaaagagca
    22921 tacttatggg cagccgttgc aaacggaaga agcagtgctg actcagttgc gccaactagc
    22981 tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat gggaaaacag tagttttgaa
    23041 ggtcaggtac acggactatt ctactgtgac caaaagagtg acattgccag aatatatcta
    23101 taaaaaagaa gcactctttt atcaagcaag ccttatatgg gaggaaatct tgggagtaga
    23161 aaaaggaatc cgattgttag ggattacttt gacgaatctg gacccgatga cttatgaaaa
    23221 catcgtcctt ccattatggg aaaatcaaga aatataaaaa cagctttgag agttgaaacc
    23281 ggaaatccgt tttctctccc aaagctgttt ttatatcaag tcttgctttt tgatttgttc
    23341 ataggtttct gttgcatccg tacatttttc ccaaatcaca acttgattgg attctaaaga
    23401 tttttggacg tcaatgatcc gctggttgct acttccgcga aattgtagtt ttaggtcacg
    23461 tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga gttctaattt
    23521 atcttctgaa tcttgtagca attcatcaaa ggtatagcct gtccatgacc agatatcttt
    23581 tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc agacaaacat ctgtattcaa
    23641 aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca agtcagcgat
    23701 gatttgttct tctaattcag gtgtgaatgg tttgccatat cgaaaatttt gtgcagcttt
    23761 gttgaagcag ccttcgcagg caaataagca gccactaacg taaagactgt tgcggacacc
    23821 ttcaccatcg acaaaattaa acggtttgta gtcagcaata tagttttggc tgtagtcttt
    23881 tgctaaccat tcttttggtt ttggatttct cataaaaaaa cttctttcat ttaaggttca
    23941 aagatatagc tattgaagat aaaatctact cgaccacata aataagaaca aactgaccta
    24001 aatccattgt tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa gatacaaagc
    24061 catcgaaaga gggtggtgaa aaaagacttg tcatagcctc ttattctgaa taaacggtgt
    24121 tcaaaagctt gcatctgcag taataagccc aactaaacga aaaatatgag gaactatttt
    24181 tgtttagcta ttcttattac ttgatgcagg acagcttttt cacaacctca tttcatgtgt
    24241 ttcacacgtg aggaaatttc tttatggcgg ccatgtacca tcggacgagc ttgcggattt
    24301 cctaaatagc cacaagtacg tttgactaca tcacatgttt ttggatcatg atttccacat
    24361 tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc acattgatag
    24421 caatgatcga ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc aaaatcccat
    24481 acagcttcta aagctttcgg attttgttgg agcatagggt attcacagta gtggatgaat
    24541 ccacctgaac aatattttgg ataatccttc tcaaattcca atttttcaaa tggcgttggg
    24601 tttttacgca catcatagtg gaaactgttt gtataatatt ctttatctgt gatattttcg
    24661 atgatgccga atttttctgt atccaaacgg cagaaacggt cagttagact ttcacttgga
    24721 gttgaataga cactgaaatg ataaccatat tcatcccccc atgcatctgc attagctttt
    24781 aattctttga cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc ccattcgcca
    24841 ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac agtagctcga
    24901 cggtttttga ataattcatt gacagaatcg gtacgtgaaa gacgtttccc gaaagcacca
    24961 tacatatata aaattggcgc atttgctgga atggcttctt tacaacgttc tacacgatag
    25021 agaagcgcat ctttcatgat ttctaagcgg tcagacagta actgccagaa tttattctga
    25081 tctccttttg attcgatcgc gattcttggc aaattcaatg taacaacacc taaattcatt
    25141 cgtccgacat ttacttcttg accattttcg tctttccacc cttgcaagaa tgaacgacag
    25201 cccataggta ctttgaaact tccagttaat tcaacgattt tatcgtaatt cagaatatca
    25261 ggatacattc ttttcgttgc acattccaat gctagttgtt tgatgtcgta attaggatct
    25321 tcagggttta agttcacacc ttttttcaaa gtgaagatca gtttagggaa gatagctgtc
    25381 cgcttttcac tccctaatcc attgattcga atctgtaaaa ttgctttttg aatttctctt
    25441 tcaaaccagt tttctcctag tccaaatcct aatgaagtga aaggagtttg tccatttgaa
    25501 gtaaataagg tattgatttc atattccaaa ctttgcatag catcgaagat atcttttttg
    25561 gtttttgctt ttgcatattc tttttggcgt tctggcgaat cgatccattc ttccgcgtct
    25621 tttagatgtt ttttatagtt caattctgca aaaggagcaa gcaattcatc tgttcggtcg
    25681 gcagaacagc caccatattg acttgacgct acattggcaa taatctgtga gatttgtgct
    25741 gtggcggtct ggatagattt tggcgattcg acttcggcat tgccgatttt gaacccattg
    25801 ttcagcatgc ctttgaaatc aatcaagcag caattcgtca tcggtgtata aggatgataa
    25861 tccaaatcat ggtaatggat atcgcctttt tgatgcgcat ttgctatatg tggtggcaac
    25921 attttcagtc cgattgattt tccaacgatc ccggctgtca gatctcgctg tgtattgaaa
    25981 acattactgt ctttatttgc gttttcgttc acgacagttt ggtccttgtt gattagcttt
    26041 tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa aatctcgttt ggtacgataa
    26101 ttgatgtact cttctgctaa ttcatattcg tttttttcta gtaatatatg ttccacgatg
    26161 ttttgaatct catagatttt gacattttca gcaaaccggc ttttgatttc ttggacaacc
    26221 aagtctgcaa tcatttgaat tttttcataa gtaagtggtg ataatgagcc gtgaattttc
    26281 ttttctgctt tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac atggcggcca
    26341 tcgcgtttga tgactttcat ctgggatgtc tctggctgct gagtaacgac agttttttct
    26401 tcataattta ctttcattat cttcaactcc tgctaaattg ttcttgtcta tcataaaaca
    26461 aaatcgggga aggagcaact aaatatcggc tatatatagt attcaatgtg ctgtataatt
    26521 ggatttctat actatatgta gtgtatattt tattgtgaaa aaaggtgaaa acttgtagct
    26581 ggtggatttg ttttttgcat atttaatttt gctgaataaa ataattgaat aaaatttttt
    26641 attcagtcca agtaaaagtg acaaaaacat aagagcaaat tattcgtaga tatgctacta
    26701 tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa gatgatcgaa
    26761 gtgaaacatc taagtaaaag ctacggcagc aaactttctc ccgtcaaagc cttagataat
    26821 atctcattca cagtagaaga aggcgagttt gttgggatta tgggcccaag tggtgcagga
    26881 aagacaacgc tgatgaatat cttgtccacg atcaatttgc ctacgatggg cagtgtctcg
    26941 atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt tcgtcgaaaa
    27001 aaactaggat tcatttttca agagttcaat ttgatcgata ccctaaatgc aaaagacaat
    27061 atcctattgc ccctagcagt tgaacggatg actaaagagg aaatggaaaa gagagtaaga
    27121 catgtagctc aattgttgaa tatagaagaa ctgctcaaac ggtatcccga tgaactttct
    27181 gtcggacaga gacaacgaat tgcagctgct agagcacttg ttgttcagcc acaagtgatt
    27241 tttgcagatg aaccaactgg ctctttggac tcgaagtcgg ctacagaatt gttgaactac
    27301 ttgaagacca tgaatcaaaa agaaaaagca acgatactgt tagtcaccca tgatccatat
    27361 acagcaagct actgcgatag aattttattt attaaagatg gtgtgatttt ttctgaagtt
    27421 gtcagaagag ggacaagaag agagttcttt gagaaagtaa tagacatgca ggcgacgatt
    27481 ggcggaggag gaaaaatgaa tgctgtataa actagccgtt aaaagttttt ttcggcagtc
    27541 acgaggctat cttgtttatt ttttcagttt gactttatcc gtaatgatct attattcctt
    27601 cagcgcaatg acctacgatc aatctttagt cagaagagca agtcaagata cttcgataga
    27661 tggtggattg agttttggtg gatttatcat tactgttgtt ttgctttttt tcgtattcag
    27721 tgccaaccga tttttcttga atagaaggca aaaggaaata ggcatctatc agctttttgg
    27781 tatgaataaa ctgcagatat caggtatata tgtgttagaa atcatgataa ttggattgtt
    27841 cgcctgtatt tctgggatat tactcggtat tattttttcc aaactctttt caatgatttt
    27901 ggttcgaatg atggatatgg atcttactag tccatttttt atctccattc catcagtagt
    27961 agatacttta tttgcttttt tcatcatcct tttagcagta tctgtatatt caatatggaa
    28021 aatatggcgc tatccaatga ttcgaagttt tggagaaaaa gaacaagcag atagttcaac
    28081 aatgcgcttc aggacaaggc accgattact cgcagtcatc gggttactac ttttaacgag
    28141 cggatatttt ggagctagtc attttaggga aatcacttca tggatgatca gttatcatta
    28201 tcttgataca ttagttttct tttttccttt tttaattttt ttcgtttgtg tggtcggtac
    28261 ttatttgttt ttctgttata catttcggtt atttttgatc atattgagta aatggaagat
    28321 ttattactat ggtatcaatt ttttgataat gggtaacacg caagttcatt tgctcaaaag
    28381 ttggcgtaca aattctctga ttacagtcat tctcggaatt tctttaatga caattggcgg
    28441 cacggctagt atctctacta ttttatccta ccaggagaaa gtttcctccc cgatggacta
    28501 tcagtttgat gttgagacag agaaaaaaat caagccgatt ctagcagagg aagggcagaa
    28561 gataactaaa gaaatcacct tgcattataa agtcattggg agtatctata gtcaaaatct
    28621 cgaagaacct atgtttcaaa ctgctaatct gatcacggaa acacaatatc aagccttcag
    28681 aaaagttaat ccagatttgc cgaacatttc tttgaaatca cctggtcata ctgttttgct
    28741 tgatcaaatg caaactcttt ttcggaatat ttcattatat ggatctagta ttggactgcc
    28801 agataatcaa gaattgaaga ttcaacaaat gcttccaagt gtattaggtg acgaaacaat
    28861 ggtatatcta agtcctgtac tgattgtaga tgataccgtt tttgaaaaag caaaaggtgt
    28921 ggattatcaa gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa tagatgaacg
    28981 actgagcaat gacctgtcga taaagtggct caatgctgtt tattattctt atgatatcgt
    29041 aaatggaagt ctacaaggag aaattagcac aaagaaactt ccggatgacc aaatgaagga
    29101 agcacaattt agccaagaaa gctctcgttt gaatttctct tccagctatt cgaaattgcg
    29161 tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg ggatgatcgt
    29221 gattattgct actggtagta ttttgacagt ccggcaatta gcagaagcag aggaagaaaa
    29281 agaaaattat cacctattga ctaagctggg gattccacaa agaaggatca gacggatgat
    29341 cttcgagcaa aacgcactta ccttctttcc acccatgata ttagggatca ctcacgcagt
    29401 atttgcaatc aatgtcttca ctcagtatat aaaaactgcc gattactggt tggcttatct
    29461 tgtgagtggc ttattgatcc ttatctatct tttgctttat gtcattacga gtcagttata
    29521 ttgtcggatt atcgaggaat gatatacgga ataagacaag ataataaata gaactaaatt
    29581 ttgaatacca tttatttgga ataagaggtt gtgacacgat ttttgtcaca acctctttat
    29641 tttttatatt ttttaaaagt acgagaaatt ataaagaaaa aagcagtgat ttgatgtgct
    29701 atttttataa aagctatgtg acaaaactgt tagtaaaaaa acggtttctg taagctagac
    29761 acatgtttct ttttgtcaaa attagttaca ctttgtgtga ggttatatga ggagggaatc
    29821 caatgaacaa atggcgttct aatccaaaat tgaattattg gggaaaattc atcattcgaa
    29881 caatttttta tagtgcagta ctgctgttcc tgatttatct gtatcactat aaaaatattc
    29941 aaggaggaac ttttatctat aacgaatttt aggaggaaga gtatggaaat caaaacgatt
    30001 attgaagcaa tcgatgattg gggaataaat gaacctgatc gagttgctta tcaggcggat
    30061 gagacgcata cttttggtga attgaaacaa gcatcagacg cacttgctta ttatttagaa
    30121 aaaaaggtag aaggcgatgg accaattgtc gtttttggca atttggagtt tgaaatgatt
    30181 gtctcatttc taggggttgt caaatctggc catgcctatt tgccgattga agagcataca
    30241 cccaaggagc gtatcctatc gatctttcgg gtggctaaac catctatggt catcagtatc
    30301 ggggactgga tagaagatat tccttcggtt cctgtcataa caaaaaaaga atttcaagaa
    30361 atcaagaaga taccagtagg attttatccc agaaatagcg taaaaggtac agaaaatttt
    30421 tatattattt ttacttctgg tactactgga gaaccaaaag gcgtacaaat cagtcatgat
    30481 aatctagtca gctttgttca atggacgatg gaagactttg ggatcaagcc tggtatgcat
    30541 ttcttagccc aagcgccatt ttcttttgat ttatcggtat tttctattta tcctgcatta
    30601 gtttctggcg gaatgctgaa gccgttaaat aaagcagttg ttcaagactt tcgtcaatta
    30661 ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt ctactccatc atttatggat
    30721 atttgcttga tggaacctac attcaatgga gaaaatgtgc cggaattgga catgttttta
    30781 ttttgcggag aagaattgac gaaaaaaact gcagaaagtc tacttgatcg attcccaaat
    30841 gcacggatat ataatactta cggtccaaca gaagcaactg tagctatttc aagtattcag
    30901 attgaccagc atgtactaga tagttatgac cgactaccga ttggctacgt aaaaaaagac
    30961 acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa aaggagaaac gggtgaaatc
    31021 attattgcag gaccaagcgt atcaaaaggt tacctgaaca atcccgaaaa gacagctgct
    31081 gcgttctttt gtcaaaatga cttagcaagt taccatacag gggatgcagg tagactagat
    31141 gaatatgggc ttttatttta tgaaggacgt atggatcagc aggtcaaatt acatggatac
    31201 cggattgaac tcggggatat cgaacattat ctgctccaag acaaccgaat caaacaagct
    31261 gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc tggttgcttt tgtcgtatta
    31321 gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat ctatcaaaga acaattacta
    31381 gaagttgtaa tggattatat gattcctcag aagttccatt atgtcgatct gctgcctcag
    31441 acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg aggtgaatcc tgaatgatgt
    31501 tacctcatat gatcccttat gcagagccgt tttatttttt tcttttagcc attgcgcttg
    31561 taccgatcgt tctatcttta ttgatatggg aaaaaagatt accagtttat caatcagcag
    31621 tgacactttt ctttttattt gtcagctttg gaggtaatta ttggcaacag ggagcggcgc
    31681 tgattgggta tgttttgtgg cagacgatac ttgtatgggg ctattttgtt tatcggcaaa
    31741 ggaaaaatca gacaggatgg ttttttgccg ccgttatttt agcgataatt ccactgtttt
    31801 taacgaaagt gactcctttt tttgaacatg gaaaagcatc gttacttggt tttttaggaa
    31861 tctcttattt aacctttaaa tctgtacaaa tgatcatgga aataagagat ggcatgatca
    31921 aaacatataa gccatttcgt tatatccagt ttctgctgtt ctttccaaca atctcttctg
    31981 gaccaatcga tcgctatagg agatttgaaa aagatcagct ccatccgcct gttcgtgata
    32041 aatatgttga tcttttggga aaaggcgtcc aaaatatttt tgtcggtttt ttatataaat
    32101 tcattatcgg ttattatttt ggtcaagtac tgcttcctgt agtgggacgc attgccttac
    32161 agcatggtgg tatttcatgg gcattagtag gttatatgta cgtgtacagt atgtatttgt
    32221 tttttgattt tgctggctat agcttatttg cagtcggtac aagctacatg atgggatatc
    32281 agacaccaat caatttcaat aaaccttttt tatcatggaa tatcaaagaa ttctggaatc
    32341 gttggcatat gacgctgtcc ttttggttcc gtgattatgt atatatgcga ttgatgttct
    32401 tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat gtcaaatatc ggttattttg
    32461 ctttgtttct tctaatgggt atatggcatg gattgacgtg gtactacatc gtatatggac
    32521 tgtatcatgc ttgtctgatc tgtctgacag atgcatggat ccgatttaag aagaagcata
    32581 aagagcagtt gccttctaac aaatttacgc atggcttagc cgtatttttg acatttcaag
    32641 cagtatgttt tagcttcctg attttttcag gatttttaga taaacttttt tttagctaac
    32701 gaatagtgac atattaattt atataacaaa ggagaaaagg aaaatggaag aacaaatttt
    32761 atcaatctta gaagaaataa caggaacaga tgaagtaaga aaaaatcgtg atattgatct
    32821 atttgatgaa gggttgcttg attctttagc atctgttcaa ttattagtcg agctagacgg
    32881 agagctggat atccaagtcc ctgtatctga attcgagcga gaggattggt gcacaccgaa
    32941 taaaatcatt caacaagcaa ctgcattgaa gggttgagta tgaaaaagaa actttttgct
    33001 attttcggcc cggttgtttt agctgttggc ttactgtttg tgttttttac ctcctcctat
    33061 caagtagata tctctaatcc agaaacgatt agacgtgcct cttcttccat gtcagtgaac
    33121 gtgttgaaag gagatgcgat aaaaaattat gcattatctg aaaaacaata tattcctttt
    33181 tttggctcaa gtgaattaag cagaatcagt ccatttcatc cgtctgttct ggcagaaaaa
    33241 taccagcgaa actatcgtcc atttttgctg ggagcacccg ggacacaatc gttaagccaa
    33301 tatatgatga tgcgttctgc aggagatgct atgaaaaaca agaaagtcgt ctttattatt
    33361 tctcctcagt ggttcgttaa aaatggtgta aaaactgatt actttaatac gtattattct
    33421 gaacttcaaa catacgactg gttgttttca atgaagaaag taacgcctgc tgatcgctat
    33481 ttagctagaa gattattaac tttctcaaaa gtaaaagaga atgatacgtt gacggcgatt
    33541 ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat ctttgaatca gctgcgcagc
    33601 caatggaaca tgctgaagag agaggatgaa gtattcagca atattgggtt gaaagatcga
    33661 cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac aataccaaga aacagaatta
    33721 tccattttag caaatcaaat tggagaacga gaaacgacga ataatccatt cgggttgaaa
    33781 aacgattttt atacgcatcg tatccgagcc cacgagccag agctcaaaca gtcacaaaaa
    33841 aattgggatt atcgtttttc gccagaattt tctgattttc agctggtctt ggatcaatta
    33901 gcaaaaaacc ataacgaagt attgttcatc atcccaccgg ttaacgagaa atggagcgat
    33961 tataccggac tatctcaaga aatgttacaa ggatttgcca aaaagatcaa atttcaatta
    34021 aatagccaag gattcaatcg aatagctgat tttgtcaatc aagcgggtac caactatttt
    34081 atggaagaca cgatccatct aggctggaaa ggctggttgg ctgcagatca acagatcaga
    34141 ccgtttttag aagaaaatca tattactgct agtaagtatc atcttgacga tgcttttttt
    34201 agcaaatctt ggcaacatca aataccagat aaactacaat taaaataaag aaaacaggcg
    34261 agtgttgctc gcttgttttc tttatttttg aatattctga ataaaagata aaaataccaa
    34321 gtttgcctga acaaaaatca taagcaatgg tataatcaca atatgaatat agaaatgggg
    34381 gaaaacattt ggatacaaaa gaagaacagc tacttattga tttaacttct gcaaaaggag
    34441 tacctggaaa tgaagaagag gtaagagaag tattcagaga atatgccaaa ccattcgctg
    34501 atgatatttt ttatgacggt ttaggcagtg tgattgccaa acatggtgct ggcggaggcc
    34561 ccaaggtctt tatttctgga catatggacg aagttggttt tatggtaaca aaaataaccg
    34621 aaaaaggatt cttggaattc caaactcttg gaggttggtg ggggcaagtc atgcttgctc
    34681 agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca cggtgtgatt ggttccaaac
    34741 caccacatgt tttaacacct caagtaagaa ataaacctta tgaaataaag gatatgttca
    34801 ttgatattgg cgcatcaagc caagaagaag ccaaagagtg gggcatacgt cctggagata
    34861 tggtcactcc atatattgaa tacaaacgaa tgaatggcag taaatattta ctggcaaaag
    34921 catgggataa tcgtatcggg acagctgttt ctctgcgagt attagagaat ctatctaaag
    34981 aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca agaagaagta ggcttgcgag
    35041 gtgctcgaac aagtacacac cttgtaaatc ccgatattgc ttttgcttta gatactggta
    35101 cagcaggaga cactccagga atgacaccaa aagaagcaga ttcgatttta ggaaaaggcc
    35161 cacagatttt gatttttgat gcatctatga tcccgcataa aaaattattg aattttgtta
    35221 ttgatgtagc agaggaaatg gatattccat tccaatatac agtgatttct ggcggaggaa
    35281 ctgatgcagg acaaatgcat ttgacaagag atggtgttcc gtctgtagcc attaccgttc
    35341 ctgttcgtta tctgcattcc catacatcag tgattcatga agatgattat ttgaatacag
    35401 tgaaactagt cacagaatta gtgaaacggc ttgatagtga aacagttgaa aaaattcgga
    35461 gttatgaata atgaataata gaatacctat ttctgaatat cggaaaaaag cacgagtttc
    35521 tttggaaaat caatggggaa tcaatacttg gcttgttttc ttatctataa tatttacgag
    35581 cgtcatccag aacttttttg acggtttcag tccttttccg gaagaaagca tgcagcagca
    35641 cttattgagt tttctagtcg ataatttact tttattcgca ttcaactatg cattgtttta
    35701 cattgcgcta gtagttgtac ggggtggcag accaaaaagt aaccttttat tttcagtttt
    35761 ccaaagaaag tattttggtt cgatacttgg catcaatttg atcaattcag tcatcaactg
    35821 gctactcaac agtttaatct ttctgcccat gtttttaatc agcggagtga gtacttatat
    35881 gcagctgctt ttcagttcaa atgcggtacc tacgggtgtc atgccagaag attttcttga
    35941 tatcagcttt gttttatcga tgatagggat gatttttgtt tcagcactca tcatgagtat
    36001 tgttggcggt ttattccaat ttgctgcatg gacaaagttc gactatcctg atttgtcggt
    36061 tattcaatgt ttgaaatatg gctggttttt attgaaagat agaattggta cgtatatttt
    36121 gttgcagctg tcttttatcg gctggtattt gctaggagca atcgctttgt tcatcggtct
    36181 tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa ttttatgaac aagcacggat
    36241 tgaaaaagga ggtccagacg cctattttac ttagcatgta agcagtttat tcatcaaaaa
    36301 aataactttt gtggtaggat aaatctttga gggggaatgt acgatgaaat taactgtttt
    36361 aggatgctta ggagcgtatc cttataaaaa tcaagggaca acaggttatt tgcttcaatc
    36421 tgacgggttc aatttgttga tcgatgcagg tagtgctacg ctgatcaaat tacaagaaca
    36481 ccttgatcca cttgatctag atgctgtgat ccttagccat tatcatcatg atcatattgc
    36541 tgaccttggt gtattacagt attactggca acttcatcct aagagaaatc cggagacatt
    36601 gctttcaatt tatggacaca caaaagattc cattcacttt gaagaattga cgttggaaaa
    36661 tgtctcaaaa ggacatcctt attttgaagc tgaagaatta aaaattggtc cttttctgat
    36721 tacttttatg gaaacaatcc atccagtagt atgttacgcc atgcgtatcg tagaagaaac
    36781 aacggggaaa gtattcgtat ttacaggtga ttcaggttat ttggaaagtt tcgctgattt
    36841 tgcgaaagat gctgatttgt ttctagcaga tacctatctt tttgaaggaa atgaacatca
    36901 tcatgcccat ttcacttcaa aagaagctgg agaacttgcg aaatcagcta acgtaaagaa
    36961 acttgtactt acacacctac ctcagtttgg tgatttagaa caattgaaaa atgaagcgca
    37021 aaaggcagca ggcaaaggga ttgatataga acttgctgca gttgacaaag tgtttaacat
    37081 ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca cgacccaaga atcaatttag
    37141 caattgaaca atatttgctg caagaaatgc ctttagatga acctattttg ctcttttata
    37201 tcaatgaacc ttcaatcatc atcggcagaa accaaaatac gcttgaagaa atcaatcgag
    37261 attatgtgga agaaaatggc attcatgttg tccgtcgctt aagtggaggc ggagctgtct
    37321 accatgattt tggaaacctg aactttagtt ttatcatgcc tgatgatggg gattcattcc
    37381 gtgactttgc gaaagtaaca cagccaatca tccaagcact tcatgaattg ggtgttgctg
    37441 gggcggaatt gaaaggccga aatgatctag tcattgatgg catgaaattt tctggcaacg
    37501 cgatgtatgc taccaacggt cggatgtttg ctcatggaac aattatgttc gactctgata
    37561 tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa aatcgaatca aaaggaatca
    37621 aatcgatccg ttctcgtgta acgaatatca aaccatttct tccagaagaa aagcaaaaaa
    37681 tgacaacaga agaatttcgt caagaaatct tgttgaaaat tttcggcgta gagtcagtag
    37741 aagaagtaaa aacgtacgaa ttgactgatg aagattggaa aaaaatcaat cgaatctctg
    37801 aggaatatta tcgaaattgg gattggaatt acgggaagtc tccagatttc aatttctctc
    37861 gccaaaaacg tttttctatc ggttcgattg aagtccattt gaatgtctca gaaggaatga
    37921 tcaaagaagc aaaaatcttt ggcgatttct tcggactagg agatatctct gacgtggaaa
    37981 aaaaattggt tggacagaaa tatgataaag catctctatc aaaaatagtc agccaaattg
    38041 atgtgaagaa atattttggt gcgatagaac cagaagaatt gcttacctta ctttattgaa
    38101 aataaaaaat agcggaatcc acatatatgg attccgctat tttttataca ggattcgggt
    38161 aatttcgaga gagttgaaca gtgatccgat caatgaaagt atctttttcc tgcgttagtg
    38221 ttcctgtgta acgatccgct acgtctttca tcatcattaa gtcaaaacgt tcctgatcat
    38281 ctaattcttc tgtgatgcct cgactaaata ggtttgtatc gtaatgtgca ctatgttcgc
    38341 aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa ataaattttt cgatacagtg
    38401 gattttcgat ggcatgagtt tctctaaagg aatgctctaa agcgtggatc aaaagttgag
    38461 ccagatcgtc atcgttgata ggaagttctt cgggaagatc tgcttggaag gttatctgaa
    38521 tattccgttt ggcagctaac ttctggtaac gcgctaagat aagatttgtc acctggtggt
    38581 tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc taatatcttt tccacatagt
    38641 ttttagcttt tgtttggtcc gtatgcatta aatcttgcag attctccatc tcttggacaa
    38701 attccaggcg aagtgtattt acttcttgga tatgctgtcg aagctgctca taatgttgct
    38761 ccaacaaatc atttttgatt tttaagaaat ctacttgtct ccgatctttg tcggtattta
    38821 agatgtgatc gattagattg tagctgataa taacgattag aatgataata tataaaatcg
    38881 tagaccaatt gatcgttgtt tgatagatac ctaatgccgt ttggatgtaa atcaagcaat
    38941 ggataatggc taccaagacg atccaaggtg tacaagctac ataaaaacgg ttattcttat
    39001 atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct tataacacta gcaatattag
    39061 caatcggcat taattcagga agttctgcag gagtaaaagt tgcataaagc agaacgccga
    39121 taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca ttttttatag taaaacattc
    39181 ggctccaata gtagcagatg atgaaagctg acgttaagat aataaataaa tgagataaaa
    39241 aagaatgaac gaatggttca aaaagaatac cacttaaaat atcttctgat acagcttcaa
    39301 atcctaatgc caaagcaaag caacttaaga gaataagtga gtattcaaga cgtttttgtt
    39361 tatacatcaa gtagatcgta tagaaaaagg tccctatagc gataaaaaca gcgattatca
    39421 tcgtcaatac ttgcggcact gattgtgaat aaataaaact aatcaatggt ccagttgttc
    39481 ctagataaac tttcggtggc agtccagcat agttctgata aggagatgag actgtaaggg
    39541 tcaatttttt tccgatataa tttgctggta aatctatcac agccaatgag agtccgggat
    39601 tgctttcttc ctgataatct ttccgttggt ataaaatctt ttcgcctaga tatacagtaa
    39661 tccattgatg gtttcctttg attagtaaat ttgcttcgtc caacggtttt tcaagtgtcg
    39721 tttccataat gaatgtttcg tctttatcca cggtaggaat cgtacgagtg tacttgctgt
    39781 caaacttaaa atcaggatca tcttttgtat agaaaaccca cgaatctttt atttctttca
    39841 tttgattgct gtccgcagga gaaaaaataa aataaaagaa ataagcaatt c
//
LOCUS       DASJTC010000014        39766 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.014, whole genome shotgun sequence.
ACCESSION   DASJTC010000014 DASJTC010000000
VERSION     DASJTC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.014"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1483
                     /locus_tag="U4B19_000682"
     CDS             <1..1483
                     /locus_tag="U4B19_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEN2076443.1"
                     /translation="LTGIVVSLFRLSIEKLSDLVRSIYEMSREQPIYLIGLAICCILA
                     AFFVGYLVKNEPDIKGSGIPQVEGQLRGELSMNWFSVLWKKFIGGVLSVGAGLFLGRE
                     GPSIQLGASIGQGAGQLFRSPSSEEKILISSGASSGLAAAFNAPIAGLLFVLEEIHHS
                     FSPLVWLTSFASAITANFVSLYFFGLRPVLYLGDLPSLPLKYYGSLVILGVLLGILGF
                     IYQKVLLALPKFYNRLPLPAYFHGFVPFLLILPIGYLWPSTLGGGNQIVLAFGHASLP
                     IIMIIGLFVLRFVFSMISYGGGLPGGIFLPILTLGALIGSFYGNIMVDFGMDPIYIKD
                     FVVIAMAGYFTAIGKAPLTAIILVTEMVGSLNHLMPLGLAALVAYIVNDLLGGNPIYE
                     SLLERLLGNTQTASLKGARTTFDFPVTAESSLDGLMVRDFNWPKNMLLISIRRGNQEI
                     LTHGDTIMNVGDILVILTDEANLPRIKQEIEAKSSITNLKQSF"
     gene            complement(1594..3525)
                     /locus_tag="U4B19_000683"
     CDS             complement(1594..3525)
                     /locus_tag="U4B19_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2076444.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3543..4457)
                     /gene="pfkB"
                     /locus_tag="U4B19_000684"
     CDS             complement(3543..4457)
                     /gene="pfkB"
                     /locus_tag="U4B19_000684"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEN2076445.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(4454..5206)
                     /locus_tag="U4B19_000685"
     CDS             complement(4454..5206)
                     /locus_tag="U4B19_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2076446.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(5365..5574)
                     /locus_tag="U4B19_000686"
     CDS             complement(5365..5574)
                     /locus_tag="U4B19_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076447.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(5585..6913)
                     /locus_tag="U4B19_000687"
     CDS             complement(5585..6913)
                     /locus_tag="U4B19_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076448.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            7049..7204
                     /locus_tag="U4B19_000688"
     CDS             7049..7204
                     /locus_tag="U4B19_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076449.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(7278..7865)
                     /locus_tag="U4B19_000689"
     CDS             complement(7278..7865)
                     /locus_tag="U4B19_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEN2076450.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(7948..9198)
                     /gene="clpX"
                     /locus_tag="U4B19_000690"
     CDS             complement(7948..9198)
                     /gene="clpX"
                     /locus_tag="U4B19_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEN2076451.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(9445..10734)
                     /locus_tag="U4B19_000691"
     CDS             complement(9445..10734)
                     /locus_tag="U4B19_000691"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEN2076452.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(10888..11820)
                     /locus_tag="U4B19_000692"
     CDS             complement(10888..11820)
                     /locus_tag="U4B19_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076453.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(11977..12636)
                     /locus_tag="U4B19_000693"
     CDS             complement(11977..12636)
                     /locus_tag="U4B19_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN2076454.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(12639..13664)
                     /gene="sppA"
                     /locus_tag="U4B19_000694"
     CDS             complement(12639..13664)
                     /gene="sppA"
                     /locus_tag="U4B19_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEN2076455.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(13677..13967)
                     /locus_tag="U4B19_000695"
     CDS             complement(13677..13967)
                     /locus_tag="U4B19_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076456.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            14377..16182
                     /gene="glmS"
                     /locus_tag="U4B19_000696"
     CDS             14377..16182
                     /gene="glmS"
                     /locus_tag="U4B19_000696"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEN2076457.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(16254..17609)
                     /locus_tag="U4B19_000697"
     CDS             complement(16254..17609)
                     /locus_tag="U4B19_000697"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEN2076458.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(17633..18802)
                     /locus_tag="U4B19_000698"
     CDS             complement(17633..18802)
                     /locus_tag="U4B19_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076459.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(18799..19686)
                     /locus_tag="U4B19_000699"
     CDS             complement(18799..19686)
                     /locus_tag="U4B19_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEN2076460.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(19875..23555)
                     /gene="nifJ"
                     /locus_tag="U4B19_000700"
     CDS             complement(19875..23555)
                     /gene="nifJ"
                     /locus_tag="U4B19_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEN2076461.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            23753..24274
                     /locus_tag="U4B19_000701"
     CDS             23753..24274
                     /locus_tag="U4B19_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN2076462.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(24973..25538)
                     /locus_tag="U4B19_000702"
                     /pseudo
     CDS             complement(24973..25538)
                     /locus_tag="U4B19_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(25717..26373)
                     /locus_tag="U4B19_000703"
     CDS             complement(25717..26373)
                     /locus_tag="U4B19_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076463.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(26941..27876)
                     /gene="whiA"
                     /locus_tag="U4B19_000704"
     CDS             complement(26941..27876)
                     /gene="whiA"
                     /locus_tag="U4B19_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEN2076464.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(27910..28908)
                     /locus_tag="U4B19_000705"
     CDS             complement(27910..28908)
                     /locus_tag="U4B19_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEN2076465.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(28905..29789)
                     /gene="rapZ"
                     /locus_tag="U4B19_000706"
     CDS             complement(28905..29789)
                     /gene="rapZ"
                     /locus_tag="U4B19_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEN2076466.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(29924..30655)
                     /locus_tag="U4B19_000707"
     CDS             complement(29924..30655)
                     /locus_tag="U4B19_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEN2076467.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(30659..31924)
                     /locus_tag="U4B19_000708"
     CDS             complement(30659..31924)
                     /locus_tag="U4B19_000708"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEN2076468.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(32187..35003)
                     /gene="uvrA"
                     /locus_tag="U4B19_000709"
     CDS             complement(32187..35003)
                     /gene="uvrA"
                     /locus_tag="U4B19_000709"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEN2076469.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(35013..37007)
                     /gene="uvrB"
                     /locus_tag="U4B19_000710"
     CDS             complement(35013..37007)
                     /gene="uvrB"
                     /locus_tag="U4B19_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEN2076470.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            37266..38768
                     /locus_tag="U4B19_000711"
     CDS             37266..38768
                     /locus_tag="U4B19_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEN2076471.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            38770..39507
                     /locus_tag="U4B19_000712"
     CDS             38770..39507
                     /locus_tag="U4B19_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2076472.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT        11233 a         8596 c         6953 g        12984 t
ORIGIN      
        1 gctgacagga atcgtcgtta gcctgtttcg attatctatc gaaaagctaa gcgatttagt
       61 tcgaagtatc tatgaaatga gtcgggaaca gccgatttac ttaattggcc tagctatttg
      121 ctgcatcctt gcagcatttt tcgtgggtta tctggtcaaa aatgaaccgg atatcaaggg
      181 aagcgggatt ccacaagtag agggacagct tcgcggggaa ttatcaatga actggttctc
      241 tgttttatgg aaaaagttca ttggcggtgt actttcagtt ggtgcgggcc tttttctagg
      301 tagagaagga ccttctatac agcttggcgc atccattgga caaggagctg gacaattgtt
      361 tcggagtcca tcttcagaag aaaagatttt aatttctagc ggtgcaagta gcggacttgc
      421 agcagcattc aatgctccta tcgcaggatt actctttgtt ttagaagaaa tccatcactc
      481 cttttctcca cttgtttggc tgacatcatt tgcttcagca atcactgcta acttcgtctc
      541 tctttacttt ttcggcttga gacctgtttt atatctaggt gaccttcctt ctttgccgtt
      601 gaaatattat ggttccttag tgatcttagg agtacttcta ggcatcttag gttttattta
      661 tcagaaggtc ttgctggctt tgccaaaatt ttacaaccga ttgcctttac cagcttattt
      721 tcacgggttc gttcctttct tgctgatttt gccaattggt tatttatggc ctagtactct
      781 aggcggaggc aatcaaatcg ttttagcttt tggacatgct tccttgccaa tcattatgat
      841 tatcggtttg tttgttcttc gattcgtttt ttccatgatt tcttacggtg gtggtcttcc
      901 aggggggatt ttccttccga tattgacatt gggggcttta atcggtagct tttatggaaa
      961 cattatggtt gatttcggga tggatccaat ctacatcaag gattttgtcg ttattgccat
     1021 ggctggttat ttcacagcga ttggaaaagc accgctgaca gcaattattt tagtgacaga
     1081 aatggttggc tctcttaatc atctgatgcc tttaggattg gctgctcttg ttgcttatat
     1141 cgtcaatgac cttctcggcg gtaatccaat ttacgaatca ttattggaac gtctacttgg
     1201 aaatactcag accgcctcac taaaaggtgc aaggacaact tttgattttc cagtaacggc
     1261 agagagttct ttagacggat taatggtacg agacttcaat tggcctaaaa atatgctgct
     1321 aatctctata cgcagaggaa atcaggaaat cttgactcac ggtgatacga tcatgaatgt
     1381 aggagatatt ttggttatct tgactgatga agcaaacttg ccaagaatca agcaggaaat
     1441 cgaggctaaa tcttctatca caaatttgaa gcaatcattt taacgaacat ggaaaaacta
     1501 ttttacaaca actattcatc tacactactt gattctatat gattctataa aaaaagaaga
     1561 gactgggaaa aaccagcctc ttctttttta tttctatgct tctgctgctg caggagcttt
     1621 tctcaataaa ccataaatca caccagatac gactgctccg atagcaataa acaccaaata
     1681 catgaccggg tgactcagta agaatacaac gaaaatcccg ccatgaggag ccagtaattt
     1741 gatcccgaaa gctccgacta atccgccagt taaagcagaa ccaataataa aacttggaat
     1801 ggcacgaatc gggtcagctg ctgcaaatgg aatcgatcct tccgtgacaa acgatagtcc
     1861 catcacgatg ttcgtcaaac cagcatcttg atctgttttg ctgaatttat tcttaaacaa
     1921 acgtgtagca acgaaaattg ccaatggagg taccattcct ccagccataa cagaagccat
     1981 cacgacactt ccgccagttg caacagtcgt agctaaagtt gctgtaccaa acacataagc
     2041 ggctttgttg attggaccgc ctaaatctgc cgccatcatt ccagctaata atgcaccaag
     2101 taaagcagca ttcgaaccac tcatacttgt caagaaaccg ttcaatgcat cattgattgc
     2161 tttcataggg acattgacca gcaacatcaa gaaaccagta atcagtaagc caaacactgg
     2221 ataaaagagg atcgttttaa ttccatccaa tgattttggt aagttcttga ataatttacg
     2281 taaaaatacg atgacataac cagctaagaa tccaccaatc agtgcaccta agaatcctgc
     2341 accgcctgta ttagctaatg caccagcagc aaatccaacg atcaaaccag ggcggtcacc
     2401 gatacttgaa gcaataaatc ctgctaatac cggcagcatg aaaccaaatg cagcttgtcc
     2461 gatttggttg aaccagctag cagcttgatt gtaattccct agatttgata attgatcatg
     2521 tggcacgcca ataaattggt cgatcatgaa agaaagcgca atagcgattc ctccaccaat
     2581 aacgaaaggt aacatgtgtg atacgccgtt catcaaatct ttgtaaattc tttgtccgat
     2641 agtaccatcc gcatttcctt cttctgaact gctttctttt ccatcaccat ggaatactgg
     2701 tgcagtacca ctaagggcta gattgatcag ctcttctggt ttacggatac catcactgac
     2761 aggacgattg atcagttctt ttccgtcaaa gcgattcatc tctacttttt tgtctgctgc
     2821 aacgattaca ccttctgcac gggcgatgtc ttcagcagtc aatcggtttt tgattccttc
     2881 tgaaccgttc gtttcgactt tgatctccac acccatttct ttcgcttttt tcttcagagc
     2941 atcttctgcc atgtaagtgt gagcgatccc tgttggacaa gctgtaacgg caacaacgaa
     3001 ttttctttct ggatgagcag aagcttctgc agcttgtgtt tcttgttctg ctttttcttc
     3061 tgcttctttt tgctcttcag ctgtttggaa caagtcttgg acttcttctg gtgtatttgc
     3121 ttcttttaat tttcctacga aatctgcatc aatcagcaat cgagatagtg ctgctaatgc
     3181 ttgcaaatgt gtgtcgtttg cgccttctgg agctgcgatc atgaaaaaca gataagttgg
     3241 ctgtccgtct aaggcttcat aatctactcc tttttggctt tttgcgaata aaactgttgc
     3301 ttctttaact gcactgtttt tcgcgtgagg catggcgatt ccatcaccaa gacctgtaga
     3361 agtttgtgct tcgcgggcta gaattccttc tttatatgtt tcgatatcgg aaatccggcc
     3421 ggcatcaaac attttttgga ccatttcatc gattgcgcct tttttatcag tcgcttgcaa
     3481 atccatgatc atcgcttctt tgaccattaa atccttgata ttcatgatta tcctccttga
     3541 aactaatcaa tttttgtgat ttcaacttct ggcagcaact catcaatcaa ttcgcgagct
     3601 gccaagtcat cagaaaaagc tgttgcgctt ccgcaagcaa ctccccattt aaaggcttcg
     3661 attggatctt tcgtacggct gtaagcccca ataaagccgg caatcattga atcccctgca
     3721 cctactgaat ttttcaatgg gcgaactaat acattcgttt tataagttcc ttgttccgtg
     3781 aagagcaaag caccgtctcc agccattgag atcagcacat tctgtgcccc ttcagctagc
     3841 aattttttac catacggaat gatttcttct acagattgga aggttgtatt gtaaagctca
     3901 gctaattcgt gattgtttgg tttgatcaac aatggtttct taacaagtgc attttttagg
     3961 tcatcacctg ttgtatcaat cacgaaatct gcaccttttt ctcgaatgat atcaatcagt
     4021 tcttgataaa atccttttcg caggctagcc ggcgcgcttc ctgacaatac gacgatatct
     4081 ccttgtccta ctgttcctaa tgcttgcttc aactcatcga tttcttcatc tgaaatagat
     4141 gggcccaaac cgttaatttc agtttcagta tctgatttta gtttgatatt gattcttgta
     4201 tcttctttta cggttgtgaa caaagactgg atttgttctt tttcaagcca cttcgaaata
     4261 aattcgccag tgaaccctcc taaaaaccct agtgctgtag attgagaatc gatccgtttc
     4321 aaaatacggg aaacattgat cccttttcct ccaggtaact tgaagtcttc cttcattcgg
     4381 ttcaaatcgc ctagttttaa cccatctacg cgtacgatat aatcaattga tggattaagt
     4441 gttactgtgt aaatcattgt attgcctcct taattgttgt cttttcacgg aattcattaa
     4501 cgaattctaa tggacaacgc tcggttatga tcgtggctgc ttccaaaggt gcaacagagg
     4561 tgaaggacaa ctggttgaat ttggtatgat caatcaatac aaatgtctct tcggaatttt
     4621 tcatggctaa acgtttaaca gctgcctctt ctgggtccgg agtcgtgaat ccttgttcaa
     4681 gatgggcacc gttcattccc ataaatgctt tattaaaatg aaaatttttt agttgttcta
     4741 tcgcattaga tcctaaaaca gcattagttg ccagctttat cgttccacct aaaataatgg
     4801 tagcaagctc caaatcaact aaatttgccg catgttgaac ggaatttgtc actactgtga
     4861 tctgttttcc ttgcagaaaa ggaatcattt ctaacgtagt agagccagcg tctaaataaa
     4921 tgacatcttc gtcattaatg gtttcagctg ccgatttggc gatttgctgt ttttcgtgaa
     4981 cgtttttgag tgatttttca ctcatattct gctcaaaatt caataattgc tctttcttag
     5041 ctccgccatg aactctttct agaaacccac tattctccag ctcttgtaaa tcacgtctta
     5101 tcgttgattc agaagcatta aaaaggttta caagatcttg tgatttcacg attttctgct
     5161 gttcaagcaa gtctaggatt ttttgtctac gttcctctgt aagcattttc acgatcacct
     5221 caatacaaat aatagcatat tttttttcta ctcgcaatat aaatcagtca aaaacgttca
     5281 taaaaattat aaatcagtca aaaaaaaata aaaaaccagt caaaaagact ggttttttat
     5341 ttttttgtct atcatcaagc cgttttatcg ttttttattt tcgcttcttg tttttttcat
     5401 ataaacagaa gcaggttttc tttccttatg aagaagataa taataaagtc ccatcccgcc
     5461 aaacatgacc aaaaaaccga tgactgatcg aataggaaaa ttaggaacaa aaaagagaca
     5521 gacggataat aatgatgccg gaaccattat tcgataatag agttgtttat ccattttctt
     5581 cacctcatat tttttgtaaa gtaatcaatt gttctaattc tcggtaatct aaaaattgca
     5641 cacgcagatg ctgctcacct ttcttgacat cgaattggat acttcgccgc tttttcttta
     5701 ttcgtttgaa ggtaacatca gagagtctat gtttgatttc ctttaacggt aattctgaca
     5761 gggattctga aggaacatag attcttaagg ataaaagata ctcttgatat ttttcatctg
     5821 ttacctctac ttctagatat tccctaatat aataacaata agcttcgatt ccattatcca
     5881 aagcaacagt cggatcgata actttatagc acatcatgcg cgagcgattc tcgtcaaccc
     5941 aaggaaaata cgcctgagca tttagtgttc cgcgaacacg cctttcttta taatctcctt
     6001 gttgaatatc ataacttttt gttacaaaaa atgaagcatt ctttttagga acaggttgca
     6061 tttgtgcatt ctgatactgg acagattgga tcgtatggtc agtgtaaatg tattcaacga
     6121 atggcggttt caataaaccg acaccattgt cccaagcaag atgatcaaac ccaagctggt
     6181 ccctaaaata ggattgaaac atttgattgt ttggcttttg ccagttaaag gggtctcgat
     6241 aaagctggat acatatgact tcgttttcta attccttcac tttttctact tgttggatgt
     6301 cgagaaaaat cggtttaggg atgatttgat gataatagcg actataatac atttcccaac
     6361 acgaagtgaa acataaacct tggtaaaaaa gatcgtatcc tggaaattgg ttgcaatcga
     6421 ctactacttc cccgttttgt tgtttcattt taggtaaatg ttcgatttca tcctttttct
     6481 caaacaatag ccgttgagag atctgttttg tattgtgata gataaactcg ctataagctc
     6541 gcatataggc gaacacccca tgagcaatca tttttttctg aatgaagtct cgaatcaaaa
     6601 attgcttttc tttgaaaaca gagatagaca atacattttt tatcaataat tgattatctt
     6661 ccaagcgaaa actacattcg ttctcgtcat ttcctacaac taaccaactt tcgtctctta
     6721 ccgatgctag cattattttt tcttgaagct ttcgaagatc atacggatcc gcttgttgtg
     6781 tgaaagagcg gataaatgtt ggctggatca acagacgtgt caaaatagcc caatcgctta
     6841 tttcctgttt tttttcttct tcatttaagt ctgtgagctg gatagtcatc aataatttat
     6901 cttcgccaat cattttctca cctctttaaa atctttttat aatttaatta taaccaacga
     6961 taaagaaaaa ctaaaacttt atgtcttaga aaccaatttg gacgattaca tgatagacta
     7021 agtaaaaaga aacaaagagg tgtttacaat ggggttgaaa tttgaacgta cagataattt
     7081 agataaactt ttcgaatcat ttgctgtcga tccggaaaaa acagttgcac caaaagaaga
     7141 gatcgatcga tttctaaaac cacagccaca aaatgaaaag aaaaaagaca agaaacaaaa
     7201 gtaagttaag ctcaaaaaaa gacttaccag ccaaactttt atgaaagagt ttgactggta
     7261 agtctttttt attcttttta tgattcaatt gcttgttcga ttgctgccca cgcttcttct
     7321 tttccctgtt ttgtaacgga agaaaagaga ataaacgtgt cattgcgatc aaaatccaac
     7381 gcttttttga tttcgctctc atgcttgttc catttaccac gagggatctt atctgcctta
     7441 gtggcaacaa caattacagg gatatcataa tacttcaaaa attgatacat ctggatatct
     7501 tcttgagaag gcttatgacg caaatctact aaagaaacga ccgcacgtag ctggtctcta
     7561 gtcgttaaat atgtctcgat catctttccc cattttgctc gctcagtttt ggatactttt
     7621 gcatacccat atcccggaac atcgacaaaa tgtaatgcgt tctcgatcaa ataaaaattc
     7681 aatgtctggg tctttcctgg cttaccggaa gtacgggcta aattctttcg atcaatcaat
     7741 gtgttgataa aagaagattt ccctacattt gatcgtcctg ccagagctat ttctggcaat
     7801 tgtgtatctg gatattgttt tggtgccact gcacttatga taatttcagc ttgatggact
     7861 ttcatggtct ctctccttaa caaggtgatg aataaagcaa gcggctgtcg caaaagccaa
     7921 aatgactttt acggcagcct cttttcatca acccgctttt ttctctgaat ctttatagat
     7981 tactgttggc tcaccagaca attcagctgc ttcttttgtg ataatcactt tttcgatgtt
     8041 ttcatttgaa gggatatcaa acatcacatc catcatgatt tcttcaatga tggaacgaag
     8101 tcctcgagca ccggtattgc gttcgattgc ttttttcgca atggctctca acgcttcagg
     8161 ttcgaattca agttcggtat tatctagaga aagaagtttt tgatattgtt tgactagcgc
     8221 atttttcggc tcagtcaaga tccgtaccag atcatctgtt gtcaatttct ctaaagctgc
     8281 cataacaggt aatcgtccga taaactcagg gatcaatccg aattttagta aatcttcagg
     8341 aatgatatgc tgcatgacac tttcgtcttc agaaagtttt tggttttgtg taccaaatcc
     8401 aatcgtcttt tcgcccatac gattcttcac aatcgtttcg atgccatcaa atgcaccacc
     8461 tacgatgaac aatatgttcg tcgtatcaat ctgaataaat tcttgatgtg gatgtttacg
     8521 tcctccctga ggtggcacgc ttgcaacagt tccttctaga attttcagca aggcttgttg
     8581 gacaccttcg ccggaaacgt cacgagtaat agatacattt tcgcttttac gcgcaatttt
     8641 atcgatctca tcaatataga tgatcccttt ttctgcacgt tccacattgt aatcagcaga
     8701 ttgaagcaat ttaagcaaaa tgttttctac atcttccccc acataaccag cttctgtcaa
     8761 acttgtcgca tctgcgattg caaaaggtac attcaatgtt tttgctaatg tttgtgctaa
     8821 aaatgtttta ccagaccctg taggtccgat caagcagata ttgctttttt gcagttcaac
     8881 atcatctgtg ttttcttgtg cttgctgatt gacacgttta tagtgattgt atacggcaac
     8941 agctaacgag cgttttgcgc gatcttgtcc aatcacgtag ttattcaata cttcaagaat
     9001 ttctagtggt ttagggactt cagtgaattc gcgtacagct tcttcataaa attcttcatc
     9061 aatgatttct ttacataggt cgatacattc attacagatg tagacccctg gaccagctac
     9121 gatctttttc acttcttctt gggttttccc gcaaaaagaa cagcgtacgg tctcattgct
     9181 acttggattg tcatacatgg tcttcacccc ttaacttgca aaaagtatgc aagaaactat
     9241 caaagacttt acagtctttt actatcataa catagtttag taaaaagaaa aagtgtttaa
     9301 caaaggatca actgacctat acctttgcat tttttactct taacaaaaaa taaggaactg
     9361 tgacaaaatc gtgtcacagt cccttatttc gaataaacaa ttttctagaa gtagcttctt
     9421 cagaaatgat ttattacttg atttctattc tttcgcttct gtcgctgtac ctgtgatcaa
     9481 ttcgattgct tttttcattg tcacatcatg ttccagcata tcttctgtca acacgcgttt
     9541 gatttgttcg attggcatgt tgtatgtttc tgataattct tggatttctg cattgatttc
     9601 ttcttctgtt gcttgaagat tttcagcagc tgcaattgct tcgatcacaa gatttgttct
     9661 tgtgcgcatt tctgcttcac cttcgaattg tttatgcaag tcttcttctg tagaaccagt
     9721 taattgatag tacatttcag gagagatacc ttgacgttgc atgttgttca agaattcgtc
     9781 cattgaacga tgtacttcat catgaaccat cacgtgtggc aattcaacga tttctgcgtt
     9841 ttcaacagcc atacggatcg ctgcttcatc ttttgcatcg tccgctgctt tttcttttgt
     9901 ttctactaat tctttgcgat atttttcttt caattcgtct aatgttgata cttcgtcatc
     9961 aacatctttt gcgaattcgt catctaattc aggcacttct tttgctttta cttcatggat
    10021 tgtcactttg aagactgctt cttttccagc aagatcttct gcttgataat cttctgggaa
    10081 agttacattt acatctagac tttcacctga tttatgacca actaattgat cttcaaaacc
    10141 tggaatgaat gagcctgaac ctaattcaag tgagtaattt tcgccttttc cgccatcaaa
    10201 cgcttcgcca tctacaaagc cttcgaagtc gattactact gtatcgccat tttcagctgc
    10261 agcatcttct ttgataacta attcagcttg tgcttcttgt tcacgtttga tacgtgcatc
    10321 cacgtcagca tctgttactt cacggtcttg tttttcaaca actagatctt tatattgtcc
    10381 taattttact tctggtttca ctgttacttc agcagtgatt acccagtctt gacctttttc
    10441 catgctttca acatcgattt ttggttgaga aacaggatcg atgccagctt ctttgacagc
    10501 agcttcataa gcttctggta atacagcatt caatgcatct tcatataaag cttcttcacc
    10561 gtacatacgg ttgaatactt gacgagaaac tttcccttta cggaatccag gtacgttcaa
    10621 gctttttttc actttattaa acgcagttgt taatcctttt tggatttcgt cttgcgcaat
    10681 cgtaaatttt aaaacaccat cattcgtgcc ttttttttca aatgttgcag tcatttaatt
    10741 ccctccgagt ttagaaattc attttataca tcaaaaacgt ttcatgcata cctttaaata
    10801 gtacactaac cctttcaaat tgtaaacata ttgaatggat agaatccttg tttttcataa
    10861 catttttatc tttttcactg ataaaatcta atggaagaag atcatttgct gcaaaatttt
    10921 ttctcttttt tttaaaaaat cttgtttttc atcttctgta ccttcataca tcgtttttcc
    10981 tgtacacgcc tctacagtca gttgaaacca gcgcttcggg tcttcgatgt atgtatcata
    11041 gacaggataa acaattgcag attgcaacgt gaattcttca tttaacacgg aactaagcag
    11101 cgcatcttca ttaccgtaac gatctgaaaa tacacataat tgctggtaac ttgcctgctg
    11161 ctgcggcttt ggcagatctt ttggtatgac tttgactaac ttttcttcaa tcgtcaaata
    11221 gaaaacagga tggcattcgt tcaattgcct aagtgtctcc agtaattttg cccttgctaa
    11281 aatatggacc tctggcgata caataagttt actcgctgtt tggataaact catgataagg
    11341 aagctgttcg atctcatcaa tagacgcaag ctgacgtgca ggttccaacg aaggaagttc
    11401 ctccgattta ttgtggagtg ccttgatctt ctgcctctcc atttgtccgg ataattcttc
    11461 tgcaaattgg attttttcaa tgacccgctg ctcgaaagct tcataaaaat ctgtacttgc
    11521 tatcaatttt ctagcatata aaaaatcttg tgtactgatt aacaagtcaa aataaaattc
    11581 agcaaactcc tcattttgaa gatactgttt ttcatgcaag agtgcttgct tcaatgcttc
    11641 ttttttctcg tccaactcgt aaagacaaaa aacaagcagg ctattagcct caaagctttc
    11701 tttcaactcg taagctcgtt gaaataattc ttttgcctgc tgatattgct ggtttgctag
    11761 tgcttgttgt gctactcgca ttaaattctc atatttttct ggaaaatcaa tcggcttcac
    11821 gcagtgtcct cctcttgctc atgttcagct catgttcaat agtagcaaaa caaaaaaagc
    11881 atgacaatta aaaataactg aagcaccctc taaaagctag attttagatc tgacttttag
    11941 aaggtgcttt caactgttac tgctttttat tgctcatcag ctgttgactt cctggttgaa
    12001 tgctttgatc agattgatcg taacaacact tgtattagtg aaaaaatcgc ctagatgttg
    12061 ctttttctga ttaaagattg ctggaagata ccctaacatc agcaaaggaa attttagaat
    12121 gaaccggcaa gcaccttctc taactaaaac ggttgaccag ctaagttctt cttcatcaaa
    12181 gctgatgact cgaataccaa agatcatttt cccaatcgtt tgaccatgat tgaatttcgt
    12241 caataaaata aaatacgcta gataaataag taaggctgtg aatccgtaga tactcagata
    12301 agaatctgaa aaattaagcc aacctagatt tttggcaata ccaagtgtta aacgcgtaat
    12361 agctccgata cacaaaagat cgaccaagaa agcaaacatt cggataaaaa aacctgcata
    12421 gaaatactta ggaaaatcat ttgcttttgg cggttcattt cttgtgtccg tatacttttt
    12481 ccatttacgt tgtttctttt tgatttcttc gggcgtcaaa ggctctcttt caaaagagct
    12541 cagtaccttt tgggaaaagt tggagtcagg tcctacagca ggctgctgtt gcttatcttt
    12601 ttcttgttcc ttattttcat ttgaaagctc tgacatgctt actcacctcc gtaataatac
    12661 attgcttttg gtgattccgc tgtccctaga ttctcaagaa cggataagat ctgactagtc
    12721 tcagaaggct tcaatccctg gaattctgct agtttacttc ctaaccatgt actagcaaac
    12781 ccagatggcg tggaagaata ttctactagt tccgcatctt caagctgttg ttcctttcgc
    12841 atagctttta aagcatcttc aggaaatccc aattcatcga ctaaacctac ttcttttgct
    12901 tggacaccat cataaatcct tccatcagca atttttttga cttcttcttc agatttattc
    12961 cgtccctcgc tgacgattgc tacaaaacga ctatatgcgc tgtcgatata tgcttgaagc
    13021 accgcttctt cttcttttgt ctctggtcgc gttcctgaac ccatatcttt caacgctccg
    13081 cttttaactg ttgtatcttc cacacccagt ttttctagaa gtcctgaata attcaatcct
    13141 gacatgatca caccaattga gccggtcact gtttcttctg ttgcaaagat tttatctgct
    13201 tgagcagaaa tatagtagcc accgctagct gccatgtttt tcatgctgac atacattggg
    13261 atatcatgtt ctttacgaat cgtatccaat ttcttagcga tttccgcact ttcgtagaca
    13321 ccgccaccag gagaatttac ttctaaaaag atacctttga ctgttggatc ttcttcaatc
    13381 gccttcagtt gttccataaa atttttatga ttgtaacctt cagaagcaaa taaacctgaa
    13441 gaccctcccg cagcaatcgt tccatctact gataatttta cgattttttg cgtactgtct
    13501 ccctcttcca acacatttga agctagttct tcatccccat ataaccaact gttcaatccc
    13561 tcaatttgat cgttttcctg aggcttagtc aaactagctg aaactaacga aaccacaaat
    13621 aatcctgcag caatcagaac tgctgcccaa cgtctcttat tcattctttc tcctttctaa
    13681 tacaagtttt ttatgacgat cacagtttct gactgctcag aagtgtcgaa ccgctctgct
    13741 ttttcttccc gcgcataccc ggaaaacgac tcttttgtca attcatcttc taccgtaaac
    13801 tcacgagctt cagcaacttc caaggcatca ggtgcgattt cttggatgct atcccagcca
    13861 gtatctaaaa atattttcgt atcatctgat acaccagctg atgcttcgaa tttggacaat
    13921 tgttttttta acattcccag tgtcatgatc tcaatttttg aatccatttt tactcctcct
    13981 ctatccgatt actttctatt gtaccgaact cctgaaaaag atgatagata aaaacccatt
    14041 attttttgac ataaaaagag catttgacaa agcatttgat atataccaat ttatttttta
    14101 ttcttttttt gttgacaaga acattcgcaa aaggttatga ttttaaatgt ttccagataa
    14161 aaaacaaaag aggaggatag ttataccttt taggatagcg ccaggtctgt gtaaacagat
    14221 gacgaggaga gggtttatcg aaagattcgg cggatggccc tagggactgc actctacaga
    14281 aaatccacaa aaaatatctg caaagataaa acaaaaggat tttcctgcag ctggaagcat
    14341 tcatacgaat tcattataga aagtaggatg ttttttatgt gcggaattgt tggaatgatt
    14401 ggattaaaaa atgtaacacc aggattgatt gacggtctag aaaaattaga ataccgtggt
    14461 tatgattcag ctggtatctt tgtctcagat ggcacaactg attatctggt caaagcacaa
    14521 ggcagaattc aaaatttgaa aaacaaaata aatattgata ctacaggaaa tataggtatt
    14581 ggtcatacac gctgggcaac tcacgggcaa ccttctgaag aaaacgccca tccacatact
    14641 tcccaaagtg gacgcttcgt gctcgttcac aatggcgtaa tcgaaaactt tgaagaactt
    14701 aaaattgctt atttagcaaa tgaccacttt attggtgaga ccgatactga gattatcgct
    14761 catctaattg aaactttcgc gaaggatact acaacgcaag aggcgttttt aaaagctttg
    14821 cgggtcatca aaggttctta cgctttcgct ttgattgatc ggacagcacc tgatgttatt
    14881 tacgtcgcta aaaataaaag tccactatta atcggactcg gcgatggatt caatgtgatt
    14941 gccagcgatg caatggctat gcttgctcat acgaaggaat tcgttgagat tgaagatgaa
    15001 gagatggtta ccgtcacatc agaaaaagtt attattcaaa attttgcagg tgacatcgta
    15061 gaacgttctt cattcgaagc acaagtagat tcttcagata tcgagaaagg tacctatcct
    15121 ttttatatgc tgaaagaaat cgacgaacag cctattatca tgcgtcgtat cgctcaaaaa
    15181 tacgtaacag aagacaatcg tgtctctctt gatcaaaagc tggtcgatac attgtctgat
    15241 agcgatcgaa tctatatcgt tgcttgcgga actagttacc atgcggggtt agcaggtaaa
    15301 cagactctgg aaaaattgac acagatccct gttgaggttc atttagccag tgaatttggc
    15361 tataacacac ctcttctttc aaaaaaacca ttctttattt tcttgagtca aagtggcgaa
    15421 acagcagata gccgccaagt tctggtaaaa atcaaccgtc tcggctaccc ttcacttacg
    15481 attacaaatg ttgctggttc tactctttcg cgggaagcat cctttacact gctattgcat
    15541 gctggacccg aaatcgcagt agcatcaacg aaagcttata ctgcacaaat agctgtacta
    15601 actttattag ccaaagctat cggtgataaa aaagaatttg aaacttctct tgtattcgat
    15661 atcactcatg aattgagttt agttgctaat gcgatggaat cggtcatcgc ccaaaaagat
    15721 tatttagaag agttggcaga agtatatctc agcgatacaa gaaatgcctt ttacattgga
    15781 cgtggcgctg actacaacgt ttctcttgaa gctgctttga aattgaaaga aatcagttat
    15841 atccaggcag aaggttttgc cgctggcgaa ttgaaacatg gaacgattgc tttgatcgaa
    15901 gaaggaacac cagtgattgg aatcatcagt gaagaagtga ctggtgcgca tactcgagga
    15961 aacctgaaag aagtcgaaag ccgtggcgct aaaacactag ttattgtctc agagggatta
    16021 gaaaaggaaa gtgaccagct ggtgctacca actgtccacc cttatctaac tactctaacc
    16081 acagtcgtac cgactcaatt acttgcatat tacgcaacgc tattacgggg attcgacgtt
    16141 gacaaaccac gtaatttagc gaaatcagtg acagtagaat aataaaagaa caaggaccag
    16201 tgtcagacat ctgacactgg tccttgttct tttatttttt atgaaaatat ctcttaatcc
    16261 agtccgattt cttctttgac tactgccgcg attttatcga catagtaatt aactttctca
    16321 tcagtaggcg cttctgccat cactcgaagt aatggttctg taccggaagg acgaactagg
    16381 attcttcctt ctgatcccat ttcagcttct gcctcttcaa tcactttttt gattgccgga
    16441 acatccatcg caccgttttt atcacttact cggatattca ctaatttttg cggatagatt
    16501 gttacctctg ctgcaagctc tgataatttc ttacctgtct gtttcatgat attcagtaac
    16561 tgaattcctg aaagcatccc atctccagtt gtgttaaaat ctaagaaaat catgtgccca
    16621 gactgctcgc cgccaaagtt ataatcgttt ttgcgcattt cttctacaac gtaacgatca
    16681 ccaacttgtg taacgacatc tttcaaaccg attccttcca ccgctttgtg gaatcctaag
    16741 ttactcatga cagtcgtaac gatcgtatct tttttcaatc gatttttttc tgccaagtat
    16801 ctagcacaga taaacatgat tttatcaccg tcgacaatgt ttccaagttc atccaccgca
    16861 ataatacgat cgccatcgcc atcgaaagct agtccagcat ctgctccttt ttcaacgacc
    16921 atctcagcta aacgttctgg atgggtagag cctacgccat cattgatatt taacccgtct
    16981 ggatttgtgc ccatggtata aaaatctgtt tcaagatcgg caaacaaacg attaacactt
    17041 gttgaagttg ctccgtttgc tgcatcgaca caaatggtca aaccggaaag gtcaccgttg
    17101 atcgtttgta caaggaattg agaatatttc aataagcctt ctggaaattc ttctacagtt
    17161 cccaatcctt cagctgatgg acgaggcaat tcatccactt cagcatctaa taatgcttca
    17221 atttctagct cttggtcatc tactagttta aaaccatctg caccgaaaaa tttgattcca
    17281 ttatcttgtg ctggattatg agaagcagaa atcatgaccc ctgcgcttgc tttttgcaaa
    17341 cgtgtcagat aagcaacacc tggtgtagag atcacaccta gctggaacac ttcgataccc
    17401 accgataaaa gtcctgaaat cagcgcttct tctaacattt gacctgagat tcttgtatcc
    17461 cgccctacta acacgcgtgg gcgttttcct tcttcttcat gctggcttag gacataacct
    17521 ccgcaccgtc ccagcttaaa tgcaagttct ggcgtaagtt ccttgttggc ttcaccgcgt
    17581 acgccgtctg ttccaaaata tttacccata gttgtaaaat tactccttta acttaatttt
    17641 ctgaggaatt tgttctgctc atttgtgttg attcgcttgt ttctgatgtt tcttctgtac
    17701 ttgtggtcga gctgcttgtc ccagattccg aactggaaga aatggttgtt gtcgatggtt
    17761 ccgttcctga atcagcagat gaaatacttc tactcgtact tgtattttct tgtgtccgac
    17821 tgctcgtcga agcagagcta ctgcttgacg tttgcaacac gggtgtaatc tccacactaa
    17881 ctgattcggg cttgatactt aacccctctt ctactggtat ttcaatttta cgagttgttc
    17941 gatgattgat acctgagaca ttgatcggta cagcaatact gtcaccgatc gatgcaagca
    18001 aatcgctaga gccgcttact tctgcttcca gttttgaaaa actgaaatca tagctttcta
    18061 ccccggcagc tggtgttcct tgctgtatcc cataaagtcg taccgacttg cttggcttga
    18121 tcgaatcagc tctcactgtt acttgcactg gatcagagac gatacttaat acttcccctg
    18181 ctgcattcag cgcctgtatt gggacctcac tattaatccc atcatctgtg accatcgaag
    18241 cgtccacatc agctactact cgtttgattt ctgccaatgt tttgtcacct gtagtgatag
    18301 agactttttt aggactaacg gaagtatcat caagttcata tccagatggt gtgaacgttg
    18361 aaggaatcaa cgtttccaca tcgaaatctt ttattacctt tttctcaacc gttactgtaa
    18421 tagtcgatgg ttcgatcttt gctgtgacag cactgctcaa attttgaatt tttagcggaa
    18481 cctcatgtgt tccttcgccc agcttagtga tatctgctgt gacgcggaac atccgtgtat
    18541 cttcgtttgc ctctgcattc aactgaactc ggtttgcact ggaaagtttg acagtcactg
    18601 cattttcata tccatggata tagtagcgct ttgaatcata agaaatcttg attggcaccc
    18661 cagtaactgt ttgattatac gcttcatttc cagaaagcgt atttggaacg gactggccat
    18721 ttgcattgaa gtacagcgca atactgaaca gcaatgcaat aaaactgtaa agaatgcttg
    18781 tccgtttctc ttttgagatc atttgcgatc ccctccctta gtcataccgt ctaggaaaga
    18841 ttgaatcacg tttttcttgt tttttgtttt ctcttcagca atcagttctc tacgcaaaat
    18901 tgccaaatat tcttgctggg ataagccagc catcagttca ttatctaatg taatgctgac
    18961 atccccagtt tcttcagaga caacgatggt aatcgcgtcg ctgacttcgc ttataccgac
    19021 agctgcccgg tgtcgagtcc cgaattcttt tggaatcaac atactttctg atagaggaag
    19081 ataggcgctt gctactgcga tctttccttc ttttacaata actgcaccgt catgcagcgg
    19141 tgtattagga ataaagatat tgatcagcag ctctccagtg atatctgcat ctaatgcaat
    19201 tcctgtctca atatactcat ctaatccagt atgccgttcg atagtaatta gcgcaccgat
    19261 ttttcgcttg gacatatatt ggatggcttt gtcaaaagac aagatcatct tctcatcttc
    19321 ctgctgttcg ctttttgatt gtttgaaaag cgaactgcgt ccaagatgtt ccagtcctct
    19381 ccggatttcc ggctgaaaaa tcacgatagc agctatcacc ccgtacgtaa tgacttggtt
    19441 catcagccaa gacaaggtgt gcagaccaat gatttccgct acgattcgga taacaataaa
    19501 aatagctaca cctttcagaa gctgaatcgc ttttgtgcct cgcagcagtt ggatcagttt
    19561 gtatacaaga taccagacaa ccagaatatc aagtatgttc accaaaaaat tcagagagaa
    19621 caagtcaccg gaaaacattt gctgccaata gtccaaatta aaaagttccc cgatttgaaa
    19681 tgacataaaa agcctctctt ctcttttgag atttcctctc tagtatatca tatgttttaa
    19741 ttttagaggc tgatctgctc gtatttttct tgagaaaaac gcataatttt caggaaaatt
    19801 gcaagtatgc gttttatttt tctctgcaat aaatattttt aaacttcaga gaattattca
    19861 ttatattttt tctgttattt ttcctttttt tcttttgtag cttgttccat ttttttacgt
    19921 cgttctgctc tggcagctct tctttcttct gcttcaaggt ccgtcgtttt ttcttttcta
    19981 gtcttaacgg gtacatcgat agattcctct ggttcaagtc gtttcttgat tttttctaat
    20041 tctcccgtca gtgcagcata gttgtaaaaa cgtgttttag cgtcttggat agtcttttca
    20101 aataaagctt ttgcaagatt tggatttgcg ttctctagcg aggaaaagcg aacttgtttc
    20161 ctcatgaaat caggcatagc agagaaatca ggttttttgt agtccaaagt cattgggttc
    20221 ttgttttttt cgaccattgc agggttatat cgatataatg accaatagcc agaagaaaca
    20281 gcttcttttg cttctttaag cgattctttc attccaccaa gcaaaccatg tgtgatacaa
    20341 ggcgtatagg caatgattaa agaaggtcca ggatactttt ctgcttcttc aaaagccttt
    20401 atcgtctgca tttgattggc acctgaagca atttgcgcca cataaacatt tccatatgtc
    20461 atcgccatca ttcctagatc tttctttgaa gcatactttc cactagccga aaatttagcg
    20521 attgccgaag ctggtgttgc ttttgaggtt tgaccgccag tattggaata gacttcgtta
    20581 tccaaaacaa gaatattcac gtctgctccg ctggcaagta catgatcgat cccgccaaaa
    20641 ccaatgtcgt aagcccatcc gtctccacca atcatccatt ggctgttttt tacgaataga
    20701 tcctttctct tgtatatatc ctccagttca gggacaattg atcgttcgct ttcgatcgca
    20761 gcttgcagtt ttgctgctct ttgctgcgtt ccttcgctct caaacatgtg taccaaccag
    20821 tcttccatca attctcgtag ttcttctgat acttttggca atacttcttt gattgaactt
    20881 gccaaagcct cgcgacgagt ctgtgccgct agaagcatac cataaccaaa ttcagcatta
    20941 tcttccaata aagaatttga ccatgcaggc ccttgaccac attcattggt cgtataagga
    21001 gaagcaggag aagcaccgcc ccaaatagaa gaacagcctg tcgcattcgc gatcatcatt
    21061 cgatcaccga acatttgtgt caataatttt acataaggtg tttcgccaca gcctgagcaa
    21121 gcgcccgaga attcaagaag cggttgattg aattgtgtag acaagacagt attctttcct
    21181 ttgactggat ttgctttttg tcttaatgtc attgcaaaag cccaattcac agcttgttct
    21241 ttctgttctt cataaggctt catcactaaa gctttttctt ttgcgggaca agcctctaca
    21301 cacagaccac aacctgtaca gtcttccact gaaacttgga tacggtattt tacgccgtct
    21361 gcgcctcgca tttctctcac gataaatcct tctggcgctt cttccatttc ctcttcatct
    21421 gctaaaaatg gacggatggc agcatgagga caaacaaagg cacattcatt acacatcgta
    21481 cagcgatcac taatccactc aggtacttct aaagcgacgc cccgcttttc catagaagcc
    21541 gttcctaaag gcatttcgcc agttgtcatt ccatttgcta caagtgtacc aacagagagt
    21601 tgatttcctt cctgtgcatt cactggttca agtatttctt gtatataacc agtgttcctt
    21661 ttattgattt tttcaggcat ctccaacgtt ctccaatggt ctgggatcgc gacttgatga
    21721 agcagttcgg tcgtccgatc gattgcttgt atgttttttt ctacgatagc cattgattta
    21781 tgtccatagc ttttcagggc ttcttctttt agcagcggaa gcacctcatc aaaaggaatg
    21841 atgtcagcta atttaaaaaa agccgtttcc attgccgtat tgatccggcg tcccaaaccg
    21901 acttcttggg ccaatctcat ggcattgatc gtatagaatc taatgttatt ttcagccaag
    21961 taacgtttta attttaaggg cagatgcgtt tctaattgtt cgtcgctcca aagagtattc
    22021 aataaaaaaa tgccacctgg cttcaatcct ttgaccaagt catagctatg caggtaagct
    22081 ggtgtgtgac aggcaacaag gtccgcatgc tcgacaagat acgctgaacg tatcggcgta
    22141 tctccaaaac gaagatgaga gacagtcaga ccgccagatt ttttcgaatc ataataaaaa
    22201 tagccttgcg catatttatc ggtgtgatct ccaataatct tgatagctga tttatttgct
    22261 ccaactgttc cgtcagatcc aaatccccaa aacttggcct ggaatgtttg cggttctgtc
    22321 aaatccagac tttcctttgg ttccaaagat tgataagtca catcatccac gatccctatg
    22381 gtaaaccgtt ttttgcgtat gcttgggtct tttttcaatt catcaaaaac tgctgagatt
    22441 tggtcgggag tcacatcttt agaccccaat ccgtagcgac caccaataac agccgggcga
    22501 agttcagaat catataacgc actttgaacg tctaataaga gaggttctcc acctgcccct
    22561 ggttctttac ttcgatcaag aacagcaatt gtttttactg tatgaggcaa cttttgcacg
    22621 aatgtttcca ctggaaaagg acgataaaga tggatattca aaaaacctgt tttccgtccg
    22681 tttgcattca agtaatcaat cgtttgttca atcgtctgtg caactgatcc catagaaaca
    22741 atgacttctt ccgcatcgtc tgctccgtaa taagtcacca agtcataaga agttcctcgt
    22801 aatcggttga tctctttcat atatttttga acgatttcag gtaatttatc ataatagggg
    22861 ttgatcgttt cccgttgttg aaaatggatg tctggattct ggttcgtacc actaacagtc
    22921 ggatgattgg gattcatccc tcgtttacga aatgaagcta atttctcctg attgatcaga
    22981 ggagctaatt cttcatattc caaaacttcg attttttgaa tctcgtgact cgtacggaat
    23041 ccatcaaaga aattcaaaaa aggtacttct ccttcaatag aagcaagatg ggcaacaggt
    23101 gctaaatcca tcacttcttg tacactgctt tcagctaaca tagcaaatcc tgtttgtctt
    23161 gcagccatca cgtctccgtg atctccgaaa atattcaacg catttgtcgt cactgcccga
    23221 ctagcaacat ggaaaacact tggcagtagt tccccagcaa tcttgtacat attcggtatc
    23281 atcaaaagca gcccttgaga agcggtatat gtagttgtca gtgcaccagt tttcaatgat
    23341 ccatggacca caccagctgc tcctgcttcg gattgcattt ctactacttt tactggttga
    23401 ccgaatacat tttcttttcc tgctgctgac cattgatcta ctaattcagc catggtcgaa
    23461 ctgggtgtta ttggatatat tgcagcaatc tcggtaaatg cataagaaat atatgcggct
    23521 gccgtattgc catccattgt tttcattttt cgcatgattc atcatccttg ttcatattct
    23581 ctcttattat ctgttccatt ataacgtaaa acattccatt ttttctttta tttataaaaa
    23641 tatttcacat atgtctattt gaattggtca tctaattttt agacaaacag tctaaatcgt
    23701 tgcctttttt tgtcagacat gtataaacta taagcgaaag aggtgtttta acatggatat
    23761 atcagtgatt gatgcaacga aaacaaatac acaaaaagga atcctttatg gttcatcgaa
    23821 tgctcctaaa aaaatggtcg aattcatcaa cctatcttgt ccgtattgcc gacaatggtt
    23881 tgaagaatct tatgagctat tagaagaagc agtccaaagc gggcaattgc agcgagtgat
    23941 caaattgttc gataaagaaa aagaaagctt gcagcgagga aacgtgatgc accggtatct
    24001 cacaatcagc gatggtcaaa aagcgatcaa agagatcaaa caaatattcg atactcaaga
    24061 cgagtggaaa catctttctc tccaagaagt agcggatttt gcagtagata aactaaagct
    24121 gacagaacaa aaagacgaac agctgagcca agctgtcatc aacgaagctg agcaagcaca
    24181 tattcgattt gttccgactg tcatcttggg taaagaaatc tttgatgaat ctatttcaat
    24241 agaggaatta aaagaattga tccaaacaaa ataaaaaaat aaggagcgta ggacaaaagt
    24301 aaagaacact tttgtctcac gctctaaaca cctataaacg gcggaagcag aagcaactcc
    24361 ttcttatttc ttggagttaa acgcttctgt cccagcctcc ttattccgaa taaatggtgt
    24421 ccaaaagctt gcatctgcca gattatctca tttcttatga caagctgagc aaattatctc
    24481 caccaaataa tttattcata tttttgactt gcgcatacta actaattctt aaattttcat
    24541 acaaaatttc tcaaattccc ttaacgagta atatttatgt acggtgattc acactagtat
    24601 cccaatcact ttttaagtta tcccgatttc aattctactt caaaaatagt tgcttttatg
    24661 tatgctgtta gttgattaga tggtcgtttg ttgagctagg tcgtttattt taaacaagtt
    24721 gtttctttag cttataccta tctcgtctac caaacgcaag atggcaaaca caaatgcata
    24781 cgcatctgta tttgccactt tgattcgtat aaagctaaca actcctaact cctctcattt
    24841 aatagttgct ctctttttta ttcagataca tttttgctga tcacagccta gtatttcaat
    24901 ggttttgaac actaaaccaa attgttccat tacaacattc actggctgat gaaatggatt
    24961 aatagatttc gattattcaa agcgattttc tattggtgga ttgtgttccc aactaacaag
    25021 acggattcca aagtcattat ataactcttc aagagttaac cctttcctgt tcccaataga
    25081 tgtatctctc ccacgctcga tataaaatat ggagtttcga atgccaagac ctggcaaatc
    25141 tttccttgtt acagttacac tgctactaac ttccccatat aaaatccagc tcctccaaaa
    25201 tgataatata tttttcgacc atcatctaac tcatgtaaca ataattgttg tgccctcttg
    25261 tcactcgcca aatgcaaacg ccacgtgcga taccatgcca tgctacctgc atctgcagaa
    25321 aatccatcaa ataatgcaac aattgtatct tctatcacaa tcctttcccc atctacttca
    25381 tattctaatc taaaatcaaa ctcccctcgg gtaatcgcag gtcttggtgg cttaggaccc
    25441 aatattattc tacttaaaac cgcattcgtt cccagatgaa acacagctat aacaacaata
    25501 atgattaata ttttgtttct atttgataaa tttctcatta ttcgcaaatc acctctacat
    25561 tctcacatac tagttcgtaa tactccttta atatcaaaat atctactgat cgtaataata
    25621 ttcgctatga attcctacta ctcgtataag aaaggccaaa tcatcagctt ttacttgctg
    25681 atgatttggc ttgtttctat ttatcgtaat tgttttctaa tcaccaaaat tgttaacaat
    25741 cggttcagat atttcccaat tcactagcct tatattatat tgatttaaca attcttcttc
    25801 atttattgta ttttgatcaa gcggaccaaa ttgttctaat aattctttgt catgtaaaat
    25861 atcccttcta gtaatgcctc tttccctttc aatgaaagaa gcactaggaa aagtatgatt
    25921 ataaccattc aacctatctc ccatgtaata attagcaggt cctggaaaat aaaatatttg
    25981 cttattatca ctaacttcta acagcaacac taaatcttct ccgctggcta aacgacttgt
    26041 ccatctacga tatctcccta tgccttcgtt cattccaaat ccatcaaatt caactattaa
    26101 cgtgtcttct atcacatgta attcttcatt caattcatac tcaagtctaa aaggaaattc
    26161 tccatatgta atctctggca atggtggtgc tggagttctc caaataaata caataactgt
    26221 gaatagaaaa aatccaacga tcgatataaa tgttatgaga cctgctcgaa gtaaaacccg
    26281 atataataaa aatgctgtat ttcgctttgc tatatctaga aaaataaaaa gtactgcaat
    26341 cataatgata gtaaaagttg taaaaaaacg catttttgcc ctccctttat aaacatcgat
    26401 tctcaaattc taaaccaaat cttggtagtt caagttcagg tataacgtat tccaaccata
    26461 catctcaatt actatttatg aacttatgat tatcactgaa taaatcactc catccccttg
    26521 ttaaaacttc tacattaatt acacctggtt gttttcgact agctaatcca atgaacttat
    26581 caaccatcct tatccaccaa ccacgagaat cttgaccaga agtaagaaga gcagttctca
    26641 aggatgatcg agcagttcct tccagttttt taattaatca ggccagttat agataaatac
    26701 ttgatcttat ctatatcagg acttctatat taagcaaaaa tttatgtaca aaactaaaat
    26761 cttcattcag ttgtctcccc gctttttata cgattgtatt aaaaacaata gaagcctttc
    26821 caaattatgt tactccagta aagatggaat tatagtttga cggaagaaaa gcaagtataa
    26881 cttttaaaaa aaactgatac attcgcatct cctgcttcat gtatcagttt tttctttgga
    26941 ttaaactgct tcttcacgca gtttttccgc aaattcattt attttgcgaa ttcggtgatt
    27001 aattcccgat tttgatatcg cacctgaggg aatcatttcc cctaattctt tcaagctgac
    27061 ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt ttttcaggaa gggcatgtag
    27121 accgaccttt gcttcgatca attcgatgtt ttcgatttgt ttagaagccg catcaatcgt
    27181 cttgttcaga tttgctgtct cacaattgac tagacgattg accgaatttc tcatatcccg
    27241 tacgattcga acatcttcaa atcggagcat tgagttggtt gcaccaatca atgttaagaa
    27301 atcagcaatt ttttctgctc cttttaaata agaaatatag ccattgcgcc gttcaagcgt
    27361 tcgagcatgt agaccgtaat aatttaacat atcgcaaatg tcttggttat gctgttcata
    27421 aatagaaaat atttccaaat gataacggct cgtttccgga ttattgactg aacctgaggc
    27481 cataaaagca ccacgtaagt aagaacgcat tttttgcgaa tttcccataa tctcgtccga
    27541 aacgtgtcca ttgaacatca taccatccat gatatcaaga tccatcaaca catcttttgt
    27601 gccttgcttc aaccggacga tatagacatt gttctttttt aatttcattt ttttacgaac
    27661 gagtagctca cttcgtacct gataatgatc tttcaaaaga gaatagatac gtctggcaat
    27721 tgcagcattt tctgtttgga cgttcaaaat aaactgctgg ttcacaatac ctaaagagcc
    27781 gttcattcgc agcaaagcag ctaattccgc tttcgcatgt tctcgatgaa cttccaaacc
    27841 agttagttct ttcttcacat ctgcagcaaa tgacatccta ttctcctcct ttctccaaca
    27901 ataaattgct taatattttg aaccgaaaac taaacggaac aattcttcta ctactttgtc
    27961 tccatcgtgg aagacaccac catctttcaa ttccaaaaag tcggtggaaa tcactcgaca
    28021 tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt actagatact catcatagat
    28081 ctctgggtcc atataatctt caggcacttt ttccgtattg accaatactg tatcgataaa
    28141 aggctgattc aaatgcttgt gtaaaacacg tatatgatcg gcatctgtga aatgttccgt
    28201 ttcccctttt tgggtcatga tattacagat atagacggtt tcagcagttg tcttgagcat
    28261 tgcttcgccg atttctggta tcactaaatt tggaaggata ctggtgaata aacttccggg
    28321 tcccaaaacg accatgtcgg cgtccataat tgctgaaacg acttttcgag ccgctttggc
    28381 ttcactttta tcatttgtgt tgcagacata cacacggtca atggttttac ggtcgatcgc
    28441 gattttagat tctcctactg cagtcgttcc gtctttaaag acagcatgca aggtcaaagg
    28501 agtttctgaa gaaggataaa catgtccatc tacatgcatc atttttgcta ataattgaat
    28561 cgcctcataa gtgcttcccc gcatctcgga aactgcggca atgatcagat taccgatcgt
    28621 atgattggct aaaaactgat cttccttttt gaaacggtat tggaaaatat cttcatagaa
    28681 ttgtggcata tccgataacg ccactaagac atttctcaag tcaccaggag gagccatagc
    28741 gatcgattct ctgattgcac cgctgcttcc cccatcatct gcaacagtca ctactgctgt
    28801 gatatctgct ccttgatttc gcaaactttt caggataact ggcagaccag tgccaccgcc
    28861 gaccactacg atctttggtt ttcttatccg gtaggttttc attttcacga ccggttcacc
    28921 gtctctttcc ttttatcctt gtcccgatgt gtcacattga ctttatagtt tttccctaaa
    28981 gcatttgcca aacgttcagt caatgccaca gaacggtgtt gaccgcctgt acagccgatt
    29041 gcaactgtca agctgctttt tccttcttta atgtatccag gcaatacagt ttccagaaga
    29101 tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg acatcacata atcatagact
    29161 ggctgatctt ttccggtcaa tggtcttagt tccgggatat aatgagggtt gggcaaaaaa
    29221 cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt atttaaaacc aaatgataca
    29281 aattcgatcc gaaatccttg gtctcccttt gatttgaatg actcattgat tttttcacgt
    29341 aattgtcgtg gtgtcaatgt cgtcgtatca atgatcacgg aagcttgggc tttcaagtcg
    29401 tctagtatcg cccgttcttt acgtatccct tcagtgatca aaccgtccat cgccattgga
    29461 tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt cgtctgaagc atctaaaaat
    29521 aagatagaag tgtcgatgaa gttcgtattt tcgatttcga tcagcatgtt ttggatttct
    29581 tcaaagaagg aacgagaacg taaatccact cctaaagcca tttttgtgac tttgcctgat
    29641 tctttgatta attcccagaa ttttggaatc aatcgaggcg gcatattatc gatacaaaaa
    29701 tagcccatat cttcaaaact ttggatagct actgttttcc ctgcaccgct catacctgtt
    29761 atgatgacta attgcaaatt atctgtcata attacgaact cctctcacat tgtgcatagt
    29821 aaaattatat cattccgggc gtatcattac cagttttctt tttcttttac aaaaagattt
    29881 ggaccaaata tcttttagga tatttgcccc aaatccaatt tttttacttt tccgatgctg
    29941 tatccaatgc cttgcttctt tcagctagcg ctcgttcaat cgtccgatct gctaaaatag
    30001 attgggcatc gatcactgga taatggttat cttcagcaaa ttcatgcatc aatgacaatt
    30061 ctgtgcaacc taagataact ttttcacagt ttaggcgctc taccgcttct tctaagattt
    30121 cgtaatatag ctcttgattc aaataatccg attccttgat ctcatgatag atcagataat
    30181 tgattttttc ttgaagaaca gtatcaggaa tcactgtttc gaatccaaga tttttgactt
    30241 cgcgttcata gattccagct ttcatacttc cttctgttcc tagaatcgct actctccctg
    30301 tcgtatgctg acggacaagt tcatttgctg cttctcttgg catatgaagg ataggaatat
    30361 ccgttgccgc ttgtaattct tcaaaaaagt agtgtgcggt gttacaagtc aacacaatga
    30421 agttcggctg caataaattt tgtttctcaa tatcatccag cagaaacggc attggatttt
    30481 cttctgatcg atctaaaata taagctgtac gatcaggaac tgttgcatga ttgaataaga
    30541 cataattcaa atattcttga tcttttgtag ctttcgttcg gtggttgatc aagcgaacaa
    30601 aactttctgt cgccatcgtg cccattccgc ctaaaatact gaaaaaattc tccatgaact
    30661 agccttcttt cacatgaaaa tactttttga atcttggaat atagttatgg aacatatatt
    30721 tcatcaacaa ccagcgcttg attgaccggt ctttttcata aaacacagta gtaccgtaac
    30781 gattttcttt gatcatttgc tcagctagtt ttttgtcttc attttcccga gcatattcta
    30841 aaaagatttt tttgggcaca cccatccata atcttgcttt atctgattga ttcgttccat
    30901 aggtcgtttc aacaaagggt ttgttaaaca ctcgatcttc tgtgacgaaa cgagccagat
    30961 tcaagccgtt caatgtcaca aagaaactgc tacgaccttg tctcaaattg atttcaaata
    31021 atttatattc tccatctcta ggatcatact tcatatcgaa attggcaaag cctgtatatt
    31081 cgattttttc caaaaatgcc ttaatggttt gatagatttt ttcgttataa tctggcatga
    31141 tcacaacgta attaccaatc gatgctggtg taggatcttc taacagtgga tgaccaagac
    31201 acatcatccg aacttgatga tcttcatcta catacgcatt tagcacacgc atattactat
    31261 catcacccgg gataaaatct tgtacaatca tttcactttt ataaccggct tcgtatatcc
    31321 ggcctaaaat taaatcgaac tcttctctag tctctaaaat aaaagctttt tttcttcctt
    31381 caaattggac agaaagatac tccacactgt ttgcaggttt caaagcaact gggaattcaa
    31441 aaggcaattc ctgttccaaa tgtccattta ctaacatttc ttctctgacg atcaatgttt
    31501 taggatacgg aagatcatat tcttcacaaa cttcataaaa gcttacttta ttgatcagtc
    31561 tttcaaataa agaataatca atatatggac agataaaata ttctgacagc tcttgtttat
    31621 gttgggagat caattctgca tatccatcac cacaagcaat caaaagatat tttttactct
    31681 tatcagaata tctttctttt gccaaacgaa gcatcgtctc gataaacaca gggtctttat
    31741 caaatcctgg aataacttct acattgacaa ttttactgta gcgggtaggg gctaactgat
    31801 ccgaagcata tgcttggcag attttgccgt atgcttcatt aaaagaacgt gccataccat
    31861 aaacgttcat gtcgcttcct aataatatag ggataaattc ttcattttca atactgttca
    31921 tcattatctc tcacttactc ataatctctt gtttttcttt tccaactaca caaggaatat
    31981 cataccataa agaaaaaagc aatcaaagga aactcctccg attgctcaat tcttttttga
    32041 acggattgaa cttataggaa gtttttgtct ataccaatca gtgtagaaag aactaccatg
    32101 taatgattct agaacactga tattaataaa acaagcaaaa attcattttt ttatgttttt
    32161 ttaactatct cagctatcta tcctctttat accattattt ttttcaaata gtgacctgta
    32221 taactttctt ttacgttaat gatttcttct ggtgttccag tcgcaaggat cgttcctcca
    32281 ccttctccac cttctggtcc taaatcaatg atataatctg ctgttttgat tacatctaga
    32341 ttgtgttcaa tcactaaaac tgtgttacca gcatctacta atctttgtaa tacatgcaac
    32401 aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt catctagtat atagaaattc
    32461 tttccattag agattttgtg caattcactg gcaagtttca tccgttgtgc ctcaccaccg
    32521 gacaatgtcg ttgctggttg ccccatcgtc acataaccta agccaacatc aacaatcgtt
    32581 tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt ctacagcatc ttctactgtc
    32641 atttccaaaa tatcagcaat gctttttcct ttgtaatgca cttctaatgt ttcagagtta
    32701 tatcgtttgc catgacatac ttcacaagga acatagacat caggcaaaaa gtgcatttcg
    32761 atcttaataa ttccatcccc gcgacaagct tcacaacgac cgccttttac gttgaaacta
    32821 aaacgccctt tcttataacc ccgcattttt gcctcgttcg tttgagcaaa taacccacgg
    32881 atatcatcaa atacacttgt ataagtcgct ggattacttc tcggcgtccg gccgattggg
    32941 ctttgatcga tatcgatgat cttttcgata ctttcgtagc cggaaattgt cttgaattta
    33001 cctggcttag cagaattctt atttaatttt tgcgctaacg atttctttaa gatactattg
    33061 accaatgtac tctttccaga accagacaca cctgtgactg ctacaaattc tcctaatgga
    33121 aattcaacac taatattttt cagattgttt tctgaagcac cagtgatctt gattgctttc
    33181 ccattacctt tacggcgttc ttttggtaca gggattgatt ttttccctga aagatatttc
    33241 cctgtcaaag aattttcatc attagctact tcttcaggag tgcctgcagc cacgatttgt
    33301 ccgccttgtt ctccggcccc aggtccgacg tcaatcagat aatctgctgc ccacatcgta
    33361 tcttcatcat gttcgactac aacgagggtg ttgccaagat cacgcatttt ctttaatgag
    33421 ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg atggttcatc taaaatatac
    33481 aaaactccag aaagattcga tccaatttgt gtagccaatc tgattcgctg cgcttctccc
    33541 ccagataatg ttccggaagc gcgactcaaa gttagatagt ctagtcccac attttccaag
    33601 aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg caattacttt ctcttgttca
    33661 gataacgtta agtttgagaa gaaatctact gctttttgaa tagacaactc atttgtttgc
    33721 ccgatattcg ttccattgat ttgaacagat agagcttggg gattcaatcg ataaccttta
    33781 catgtctgac aagttaattc tgtcatataa agacgcattt gctcacgcgt gaaatcactg
    33841 ttcgtatcat gataacgacg ctcgatattc ttcaataccc cttcgaaagt cgtttctaca
    33901 tcgcgaacgc caccaaaatc attttcataa tggaaatgga aaagatcatc tgtcccattc
    33961 aagatgatat cctgatgctc ttccggtaat tcttcgaatg gtgtatctaa atcgataccg
    34021 aaactttcac aagcctgcgc taacatttga ggataatact gagagctgat tggattccat
    34081 ggaacgatag caccttgact aagtgtcttc gttctatctg gaatcactaa atcttgatcg
    34141 acttctaatt tgacacctaa accatcacag tctggacaag ctccaaaagg cgcattgaat
    34201 gagaacagtc ttggttctag ttcgccaaca gtaaatccgc aataaggaca ggcatagtgt
    34261 tcgctaaaca acatttcttc ttcaccgatc acgtcaacca gagcatagcc gtctgccaat
    34321 cgcagagccg cctctaaaga atcaaataga cgagaacgaa tcccttcttt cacaatgatc
    34381 cggtcgatga cgatcgcgat atcatgtttt ttgtttttct ccagctcagg cacttcactt
    34441 aaatcataga tctcaccatc gactcgaacg cggacgtatc cctctttttg gattcgttcg
    34501 aatatttttt tatgttgtcc ttttttcttt accacgatcg gtgccaacac ttggatcttt
    34561 gtgcgctcag gcaaagccat tacttgatct gccatttgat ctactgattg actggtaatc
    34621 tcgatatgat cgttgggaca aatcggatgt cctacacgtg caaacaacaa gcgtaaatag
    34681 tcattgattt ccgtcacagt acctactgtc gaccgaggat ttttacttgt tgttttctga
    34741 tcaatagaaa tcgccggact caatccgtcg atgctatcta cgtccggctt atccatttgt
    34801 cctaaaaact gacgagcata agcagaaagg ctctctacgt atctccgctg cccttcagcg
    34861 tacagcgtat caaatgctag ggaacttttt ccagaaccgg acaaacctgt aacgaccacg
    34921 aatttatctc gtggaatcgt tacatctata tttttcaagt tatgggcacg tgcaccgtga
    34981 atcgtaattt tatcgttagc catgttgtcc tcctactttg aagcttttaa ttctaaaatc
    35041 atatctcgca cattagcggc tgtttcaaaa tccaatgctt tcgccgcatt tttcatttct
    35101 ttttctaatt tcatcaataa cgtatctttt tcttctttcg tcatttcttc atacgagact
    35161 tgtaccgctt cttttgtatc atcttccgat tctttcgtaa tagaaatcaa atctcggatt
    35221 tctttaatga tcgtttttgg aacgatccca tgttcttcat tgtacttctg ctgaatcgta
    35281 cggcgtcttg atgtttcatc catcgctaga cgcatagaat ctgttacttt gtctgcgtac
    35341 atgattactt taccttcttc attccgagca gctcgtccaa tcgtttggac taaggaacgt
    35401 tcactacgta aaaagccttc tttatccgca tccaagatag caacaaggga aacttcaggt
    35461 acgtctaatc cttcacgaag caaattgatt ccaactaaca catcaaattc acctaatcga
    35521 agatctcgaa tgatttctgt tcgttccagc gttttgatat cactatgcaa atatttgact
    35581 ttgatcccta attctttgaa ataatctgtg aggtcttctg ccattttttt cgtcaatgtc
    35641 gttacaaaca cccgctggtc tttttcgact cgttcattta tttcgcctac tagatcatcg
    35701 atttgtccca tgattggacg aacttcgatc actgggtcaa gcagccctgt cggacggatg
    35761 atttgttgga taacggtatc tgtttgttca tgttcatatg gtcctggcgt cgcagagaca
    35821 taaatgatct gattgacatg tttttcaaac tcttctaacc gaagtggccg attgtctaaa
    35881 gcagatggca aacggaaacc gtaatctact agcatctgtt ttctcgctcg gtcaccatta
    35941 tacattcctc tgacttgagg catggttacg tgcgattcgt ccgccacgat caaaaaatct
    36001 tcaggaaaga aatccaatag cgtataaggc ggttcacctt ctttacgtcc gtccatatgc
    36061 cgagaataat tttcaattcc cgacgtgtag cccatttcta acatcatttc aatatcgtaa
    36121 ttggttcgct gttctaaacg ttgagcttcc agaagtttgt tttcatttcg caagacagtt
    36181 aaacgctgtt ctaattccgc tttgatattc gctacggcat gctccatatg ttcatcattc
    36241 gtcacaaagt gagtcgccgg gaagattgcc acgtgttccg tttctcctaa aacttcacct
    36301 gtcagtgcat tgacttctcg gatacgttcg acctcatctc caaaaaactc cacacgtaag
    36361 gcacgttcat ctcttgaagc cggaaaaatc tccacgacat caccgcgcac tcgaaaacga
    36421 ccgcgctgaa aatctatatc atttctttcg aattggatac tgactaagtc acgaatcaac
    36481 tgattgcgat ctagttctgc tccctgtcga atcgaaacta cttgtttttg gtactcaaaa
    36541 ggcgaaccta atccaaaaat acatgaaacg gatgcaatga cgatcacatc atttctttct
    36601 aataatgaac tagtagccga atgtcgtaat ttgtcgatct catcatttac acttgaatct
    36661 ttttcgatat acgtatcgct tgaaggcaca taagcttcag gctgataata atcataataa
    36721 gaaacaaaat attcaacagc attgttaggg aaaaattcct tgaattcccc gtacagctga
    36781 ccagccaatg ttttgttatg ggcaataatt agtgttggtt tattcacttt ctcaataaga
    36841 ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta ataagatttg tgcttttttc
    36901 ccgccgacta cgccatccac taattggtta atcgcttcag gctgatcacc agccggttgg
    36961 tatttcgaga caagctcaaa gtgtcgggag gtatctctct caatcattct tcgtcctcct
    37021 tttcattcat tccttttcat tttacgacca ataaaaagaa aaggaaagca aaggcattcc
    37081 ttactaaaaa attataccac accgaacata ctttcgccac ttttttatct tattatgcat
    37141 gttacgtttt ataacataaa atgttagaaa ctcgcatttt tttcattttt tcgagcttac
    37201 agtcttgtct gattcagcac ttttcgccta taataataaa atattggaaa tttaggaggt
    37261 tgattatgaa aagaaagcat ttatttcact catttgtttt cttaatgaca ggacttgtga
    37321 cacttgcgtt tggacatgtt gcacaagctg acaacactac aacggcagag tcaagcacaa
    37381 gcgagacaat gcaaaaaata gagcccaaaa aagacgtata cgtaattgcc agtgactctg
    37441 cttttgctcc atttgaattt caaaacgcaa agggcgagta cgaaggcatc gatgtaaaat
    37501 tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg aatggactac cgtggtttct
    37561 ctgctgcttt gcaagctttg gaatcaggac aagcagacgg aatgattgcc ggaatgactg
    37621 taacagatga acgaaagaaa agctatgatt tttctgattc ttactttgat agcgggatcc
    37681 aattagctgt caaaaaagga gatacttcga tcaagtctta tgaagatttg aaaggaaaaa
    37741 cagtcggagc aaaagttggt accgaaagtg ctgaattttt aagcgaccat gaatctgaat
    37801 atggctattc tatcaaatat tttgatgctg ctgatcaatt gtacgacggg ttaaaagttg
    37861 gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg ttatgcgatc aaacaaggac
    37921 aagaattgga aacaccgatc aaaaaagaat ctggcggaaa atatgcattt gccgttaaaa
    37981 aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg attgaaagaa ttaaaaagaa
    38041 caggcgaata cgataagatt attaaagaat atgtagcgga tggttcagaa acttccagtg
    38101 agtcttccac tgctgatgaa tcaacatttc ttggacttat ccaaaacaac tatcaagcat
    38161 tgttgaaagg actttggaga acaattctcc taactttgat ctcctttgct ttggctatag
    38221 taatcggtgt gatttttgga ttattcagtg tagcaccaat acgcgctctt cgcacaattg
    38281 ctagtattta tgtcgatatc atccgaggaa ttccaatgat ggttcttgcg ttctttattt
    38341 tctttgggtt gcctggtatc gttggattta cgattcctga cttccttgct ggtgttatca
    38401 ccttgactct taacgcaagt gcttatatcg cagagattgt tcgaggcggg atcaacgccg
    38461 ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct ttcttataat cggacaatgc
    38521 aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc atcattcgtc aatcagttcg
    38581 ttatctcatt aaaagacaca acgatcattt ccgcaatcgg tgtagtcgaa ttgctgcaaa
    38641 ctggtaagat cattgttgca cgtacaacac aaagtacgta tgtttacttg atcattgcga
    38701 tcatgtactt gattttgatc acagcattga caaaattagc aaaagtttta gaaaagaagg
    38761 tgaagtaaga tggcagaaaa aatcttagtt gagcatttag tcaaaaaata cggcgataac
    38821 acagtattaa acgacatcaa tgtgtccatc aacgaaggag atgtcgtctg tatcattggc
    38881 ccttcaggtt caggaaaaag tacatttttg cgttgcttga atcgtttaga agaaccttct
    38941 gacggtgaaa ttattattga cggcgcccac ttgatggata aaagcacaga tatcaacttg
    39001 gtgagacaac atatcggaat ggtattccag cactttaacc tattccctca tctttctatt
    39061 ttggaaaaca tcattttagc accgatggac ctgaagaaag aatctagaga agaagcagag
    39121 aaaaaagcaa tcaaattgct gcagacagtt ggtctagaag ataaaaaaga tatgtatcct
    39181 gaaatgcttt ccggtggaca aaagcagcgt gtggctattg ctcgtgctct agcgatgaac
    39241 cctgacatca tgctttttga tgagcctaca tctgcactcg accccgaaat ggtcggtgat
    39301 gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga cgatggttat cgttacgcac
    39361 gaaatgggct tcgctaaaga agtggccaat cgtgtcatgt tcattgacga cggaaacttt
    39421 ttagaagacg gaacgcctca acaaatcttt gaaaatccgc aaaatgaacg gacaaaagat
    39481 ttcttgaata aagtattaaa tatttaataa aaaccaaaaa tgcccggtcg cttcatgacg
    39541 accgggcatt tttagtttct tgttttttca cttaactgta ccagccgcat cgcttgttct
    39601 cctgcacaaa caagcaatcg ctctacctgt tccatcgcat aagaaagtac catcggttca
    39661 ttgacgatat caagtacata gtcgatacca ccttcagaaa ctatgatgat tatcttattc
    39721 taaagctggc ttttttgttg gtacaaacat gacaactaca aaaaaa
//
LOCUS       DASJTC010000015        36705 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.015, whole genome shotgun sequence.
ACCESSION   DASJTC010000015 DASJTC010000000
VERSION     DASJTC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.015"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..626)
                     /locus_tag="U4B19_000713"
     CDS             complement(<1..626)
                     /locus_tag="U4B19_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2076473.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            865..1296
                     /gene="mraZ"
                     /locus_tag="U4B19_000714"
     CDS             865..1296
                     /gene="mraZ"
                     /locus_tag="U4B19_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEN2076474.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1317..2276
                     /gene="rsmH"
                     /locus_tag="U4B19_000715"
     CDS             1317..2276
                     /gene="rsmH"
                     /locus_tag="U4B19_000715"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEN2076475.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            2281..2682
                     /gene="ftsL"
                     /locus_tag="U4B19_000716"
     CDS             2281..2682
                     /gene="ftsL"
                     /locus_tag="U4B19_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEN2076476.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2683..4875
                     /locus_tag="U4B19_000717"
     CDS             2683..4875
                     /locus_tag="U4B19_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN2076477.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4901..5863
                     /locus_tag="U4B19_000718"
     CDS             4901..5863
                     /locus_tag="U4B19_000718"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEN2076478.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5877..7238
                     /locus_tag="U4B19_000719"
     CDS             5877..7238
                     /locus_tag="U4B19_000719"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEN2076479.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            7264..8352
                     /gene="murG"
                     /locus_tag="U4B19_000720"
     CDS             7264..8352
                     /gene="murG"
                     /locus_tag="U4B19_000720"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEN2076480.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8367..9587
                     /locus_tag="U4B19_000721"
     CDS             8367..9587
                     /locus_tag="U4B19_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEN2076481.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9743..11068
                     /gene="ftsA"
                     /locus_tag="U4B19_000722"
     CDS             9743..11068
                     /gene="ftsA"
                     /locus_tag="U4B19_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEN2076482.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            11091..12332
                     /gene="ftsZ"
                     /locus_tag="U4B19_000723"
     CDS             11091..12332
                     /gene="ftsZ"
                     /locus_tag="U4B19_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEN2076483.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12349..13026
                     /locus_tag="U4B19_000724"
     CDS             12349..13026
                     /locus_tag="U4B19_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEN2076484.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            13040..13639
                     /locus_tag="U4B19_000725"
     CDS             13040..13639
                     /locus_tag="U4B19_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEN2076485.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13654..13941
                     /locus_tag="U4B19_000726"
     CDS             13654..13941
                     /locus_tag="U4B19_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEN2076486.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13990..14772
                     /locus_tag="U4B19_000727"
     CDS             13990..14772
                     /locus_tag="U4B19_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEN2076487.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14793..15500
                     /locus_tag="U4B19_000728"
     CDS             14793..15500
                     /locus_tag="U4B19_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEN2076488.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15762..18560
                     /gene="ileS"
                     /locus_tag="U4B19_000729"
     CDS             15762..18560
                     /gene="ileS"
                     /locus_tag="U4B19_000729"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEN2076489.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18609..20135)
                     /locus_tag="U4B19_000730"
     CDS             complement(18609..20135)
                     /locus_tag="U4B19_000730"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEN2076490.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(20150..20797)
                     /locus_tag="U4B19_000731"
     CDS             complement(20150..20797)
                     /locus_tag="U4B19_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEN2076491.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20981..21310)
                     /locus_tag="U4B19_000732"
     CDS             complement(20981..21310)
                     /locus_tag="U4B19_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEN2076492.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            21487..22215
                     /gene="sfsA"
                     /locus_tag="U4B19_000733"
     CDS             21487..22215
                     /gene="sfsA"
                     /locus_tag="U4B19_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEN2076493.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            22231..23244
                     /locus_tag="U4B19_000734"
     CDS             22231..23244
                     /locus_tag="U4B19_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2076494.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            23244..24521
                     /locus_tag="U4B19_000735"
     CDS             23244..24521
                     /locus_tag="U4B19_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2076495.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            24584..27286
                     /locus_tag="U4B19_000736"
     CDS             24584..27286
                     /locus_tag="U4B19_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2076496.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            27438..27755
                     /locus_tag="U4B19_000737"
     CDS             27438..27755
                     /locus_tag="U4B19_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2076497.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            27785..28105
                     /locus_tag="U4B19_000738"
     CDS             27785..28105
                     /locus_tag="U4B19_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2076498.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            28213..29673
                     /locus_tag="U4B19_000739"
     CDS             28213..29673
                     /locus_tag="U4B19_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2076499.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            29741..29962
                     /locus_tag="U4B19_000740"
     CDS             29741..29962
                     /locus_tag="U4B19_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076500.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            29993..30175
                     /locus_tag="U4B19_000741"
     CDS             29993..30175
                     /locus_tag="U4B19_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEN2076501.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            30175..30588
                     /locus_tag="U4B19_000742"
     CDS             30175..30588
                     /locus_tag="U4B19_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEN2076502.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            30711..31892
                     /locus_tag="U4B19_000743"
     CDS             30711..31892
                     /locus_tag="U4B19_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2076503.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(31963..32127)
                     /locus_tag="U4B19_000744"
     CDS             complement(31963..32127)
                     /locus_tag="U4B19_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076504.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            32281..32354
                     /locus_tag="U4B19_000745"
     tRNA            32281..32354
                     /locus_tag="U4B19_000745"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32315..32317,aa:Arg,seq:cct)
     gene            complement(32423..33562)
                     /locus_tag="U4B19_000746"
     CDS             complement(32423..33562)
                     /locus_tag="U4B19_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2076505.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(33861..34496)
                     /locus_tag="U4B19_000747"
     CDS             complement(33861..34496)
                     /locus_tag="U4B19_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076506.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(34609..35244)
                     /locus_tag="U4B19_000748"
     CDS             complement(34609..35244)
                     /locus_tag="U4B19_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HEN2076507.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(35278..35739)
                     /locus_tag="U4B19_000749"
     CDS             complement(35278..35739)
                     /locus_tag="U4B19_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEN2076508.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(35869..36300)
                     /locus_tag="U4B19_000750"
     CDS             complement(35869..36300)
                     /locus_tag="U4B19_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2076509.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(36318..36638)
                     /locus_tag="U4B19_000751"
     CDS             complement(36318..36638)
                     /locus_tag="U4B19_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2076510.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
BASE COUNT        12116 a         6312 c         7522 g        10755 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccgtcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtagggtga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa aagtgttagt
      721 agaaaatcgc tttcgatttt ccaccaacac aaaatattat agaaccaata aaaaaagttg
      781 ttcccggatt tcccagaaaa ggtggtagaa agtgggggat tgtggtagac taattttagt
      841 tagtggagga atggggggct ttcaatgttg atgggtgaat tccaacataa tattgatgca
      901 aaaggacgtt taatcgtgcc ctcaaaactt cgtgaagaac ttggcgaaaa gtttgttctg
      961 acacgcggat tggatggctg tcttttcggc tatccgatga gcgaatggga aaatctggaa
     1021 gctaaactga acgaaatgcc gttggctaaa aaagacgcac gtacttttgt gcgattcttc
     1081 tattcggctg ccactgaatg cgaacttgat aaacaaggac gaatcaatat acccagcacg
     1141 ttaagaaatt atgcagcttt gacaaaaggt tgcgtagtca tcggtgtatc taaccgaatt
     1201 gaaatttggg atgagacacg atggcaagat ttttctgcag cagcagaaga gaactttgac
     1261 gaaattgctg aaagtatgat tgatttcgga ctataagaat aggaagaggc tgactaatga
     1321 ctgaaacatt tcaacactat acagtcatgt tgaaagaaac ggtagatgga ttgaacatca
     1381 aaccggatgg cgtttatgta gactgtacac tcggcggtgc cggacatagt gaatatttat
     1441 taagccaact aagttctaaa gggcatctgt atgcgtttga ccaagatcaa aaagcaatcg
     1501 atcacgccaa gctgcgttta gcttcttatg tcgaacaagg tatggtcaca tttatcaaag
     1561 cgaacttcag agaattagaa gaacgcttga gtgagcatgt agataaggta gacggcattt
     1621 tgtatgatct aggggtttcc tctcctcagc tagatgaagc agaacgtgga tttagttatc
     1681 atcaagatgc accgttggat atgcgcatgg atcagtcagc tcctttatcc gcttatcacg
     1741 taattaatga atattcctac catgaattag taaagatttt cttccgctat ggcgaggaga
     1801 aattttccaa acaaattgct cgtgaaatcg aacgagtacg aaaaacaaaa ccaatccaaa
     1861 caactggtga gcttgtcgaa atcattaaac aagtcatccc agctcccgca agaagaaaag
     1921 gcgggcatcc tgcaaaaaga atttttcaag ctgtgcggat tgcagtaaat gacgaactcg
     1981 gtgccgaaga ggcctcttta gaacaggcaa tccggttatt gaaagtcaat ggaagaatca
     2041 gcgtaatcac attccattca ttggaagaca gaattgtcaa aagtatgttt aaagaatata
     2101 gtacggtaca agatctacca ccgggacttc ctgtcatacc tgacgaattc caaccagaac
     2161 tgaaagtcat tactcgtaaa ccaatccttc cttcagagga agagctaaca gaaaacaatc
     2221 gatcacgcag tgcaaagtta cgtattgcag aaaaaataag aatgaaagag gagtagaatg
     2281 atggcagaat taaagaaaat gcaagattac gaatatgatt tacctgaatt ggtggatcag
     2341 ccggaacaac aaatgcctga ttcttcttcg aatcaagaaa tcattcttcc acaatcaccg
     2401 gcaaaacgat taaagcagat ttctgctttg gaaaaaattg ctgtggcaag cgtggcgatt
     2461 gctattgttg ctttatgtgt tttaacagtt atgctgcgca caaatatcag cggagctgaa
     2521 aaagaaatca cgtcaataca aacagaaatc aatgataaaa agcaagaaaa aacgagttta
     2581 gaacaagaaa aaaacgaact ttctcgtact gaacggatta aacaaatcgc agaagaaaaa
     2641 ggattgtcta ttaatgacga caatttaagg aaagtgaagt aaatgagtct taaaaataaa
     2701 attcggcggt ttatggaaaa gaaaaatcta aatccgatga acaatcgcaa aaaagtaggt
     2761 atcattttat ttgctacgag tattggattg ttctttttat ttgccgtccg tttctcgtat
     2821 atcgttattg gaggtcatgt tgctggtacc tcattagctg aaaaaacaaa acagttgtac
     2881 caaggaagtg aagtagtaaa agcaaaaaga ggaacgattt atgatcgtaa cggtgtggcg
     2941 ctcgcagaag atgcctcctc ttattcaatt aaagcgatac tctcaaaaac atatacctct
     3001 ggggataaaa aactgtatgt tgaagaaaag aattttgata aaatagcaga aatcctgcat
     3061 aagaatcttt ctatagacaa aaaagatgca ctgaatattt tagaggacgg cgcaaaaaaa
     3121 gagctgtatc aagttgaatt tggtagctat gggaaaaata tctcccaaga aaccaagcaa
     3181 aacatcgaag cggacatgaa aaaagaaggt gtcgctggtc tttattttgt cgaccatcag
     3241 gctcggatgt atccaaatgg cgtcttttct tctcatttta ttggatacgc agttccagac
     3301 aaagatgaga atggcttagt tggtaaacta ggtttggagt ctgcttacaa tgatatcttg
     3361 agcggtaaag atggtaagat tatttatcaa aaagataatt ttcaaaatcc tctgccaggt
     3421 acagtggcag aagaagaaaa agctgtcgac ggacaagata tctacacgac tttagacagc
     3481 agactacaaa gttatttaga aacactgatg gatcaagtaa atgaagaata tcagccagaa
     3541 gaattgacag cagtcctgat gaaagcaaaa accggagaaa tcttggcaat gggacaacga
     3601 cctactttca atcctgaaac aatggaagga ttgactggaa aagatgctat ctggcgtaat
     3661 ttcttggtac aagatagtta tgaaccagga tcaaccatga aagttttcac tactgcagct
     3721 gctatcgaag aaggagaatt caacgagaat gaaaccttcc aatctggaaa aatccaagta
     3781 gaagatgcta cgatcaatga ccatgacttt ggtgaaaaag gtgtgctgac catgcgtcaa
     3841 gctctttcat ggtcaagtaa cgtcgggatg gtcatactgg aacagcgatt aggcggacga
     3901 tggtataact atttacaaaa actgggattt ggacaaagta ctcattctgg attagatgat
     3961 gaagtaaacg gtgctttacc tacattaaat attgtcgacc gagcaatgag tgcttatgga
     4021 caagcggtag gtgtgacgaa tttccaaatg atgaaagctt tcacttctat tgcgaataac
     4081 ggtacaatga ttcaacctcg atatatcagc aaagtggtgg atccccaaac tggagaagag
     4141 cgaacaactc aaacagaagt tctagggcaa cctttttcaa aagaaacaac agaaaaagtc
     4201 cgtgaatata tgagagatgt tgtcgaaagc gaaaactacg gtagtgctta tggtgtatat
     4261 agtgtacctg gatacaatgt atcggcaaaa aacggaacag cccaaatcgc ttctgataca
     4321 gggggatatc aaacaggaga tacagcctac ctgtattcga ttgtggagat ggttccttct
     4381 gaagatccag actatgtctt gtatctaaca atgaaacacc ctaagacata tgatcgaatg
     4441 gcattggcta aaatcgcaaa tccattgatg aaacgggcaa tggattttaa agaaactgaa
     4501 gaagattcag atacagaaac aaaaacagaa aaagtatccg tagccgatta tcgtaatctt
     4561 gaagcggacg ttgcagcagc tgatgctcaa aaaagtggac tgcaacctgt agtgatcggt
     4621 aatggtaaaa aagtacaaaa acaatctact gccaacggtg atcagttgat ctctggagaa
     4681 aaactgatcc tatatactgg tggtgataag ctgatgcctg acgtaacagg atggtcaaaa
     4741 gcagatatca tgaagctggg gaaaatcctc ggtattgaag ttagttttga tggagatggt
     4801 tattgtgtca aacaagaact tgctccatat gaaaaaatca ctgaagacaa acttagtttt
     4861 actttagaag aataagaaga gaaataggag agaaaagagc atggaatgga cacaagcatt
     4921 gattcccatt gtcagcagtt gtgcaatgac aattgccgca atgccgctat ttattggtta
     4981 ttttcaaatg aaaaagcaag gtcaggcgat tcgtgaggag ggaccaaagt ggcacaatag
     5041 caaagccgga actcctacaa tgggcggctt agtgtttctg atcggctcta ttttgacagg
     5101 aatttgggtt ggagcttggc aaaaacagct aactcctact cttttcatct tgctttttgt
     5161 attagcgtta tacggagtaa tcggctttct tgatgatttt ataaaaatat tcaagaaaag
     5221 aaacatggga ctgaactcga agcagaaatt gattggacag attctagggg gaatcatctt
     5281 ttatcttgtt taccgttctg aaggttatcc aggtgtattg aatttcttcg gactagatct
     5341 acctttagga atcatttatg gtattttcgc tctcttctgg cttgttggat tttcaaatgc
     5401 agtaaatctg acagatggca tcgatggatt agttgctggc ttaggaagta tctcttttgc
     5461 agcttacggt ctaattgctt ggcatcagca tcagtatgat gtattaatca tttgcttaag
     5521 tgttttagga ggtttactgg gtttcttcgt ctacaacaga aaacctgcca agatttttat
     5581 gggagatgtc ggttcattag cgttgggcgg acttttagcg gcaatttcta taatgctaaa
     5641 ccaagaatgg acattgcttt tgattggttt gatctatgtt atggaaacag caagtgtgat
     5701 gcttcaagtt acttccttta agcttacagg aaaacgaatt tttaaaatgt ctccaattca
     5761 tcatcacttt gaaatgtgcg gatggtcgga gtggaagatt gatacagttt tttggttgat
     5821 cagcattgtg acttcgttga tcacattatg gatcgtttgg taggagatga cgggaaatga
     5881 aaaaaatatc aacatatgaa aataagaaag tgcttgtctt aggactcgca aaaagcggag
     5941 taagtgcggc caaattatta catgaattgg gcgcacttgt caccgttaat gatggaaaac
     6001 cttttgacga aaatccggaa gcacaagagc tgctttcttt agggataaaa gtaataacag
     6061 gtagtcatcc aatcgaattg ttggatgaag aattttcatt gatggtcaaa aatccaggga
     6121 ttccctacag tcatccttta gtagcaaaag cccaagaaat gggtattccg gtaattacgg
     6181 aagttgagct agcttacgaa gtagcggaat gcccaattat tgggattaca ggaacgaacg
     6241 gaaaaacaac gacaacaacg atgacaggac tactactgaa tgcaggagca gatcaaggaa
     6301 ttgcacgttt agcgggtaat attgggtatc cagcaagtgg tgtagcgcaa gaagcaaaat
     6361 cagaagataa aattgtgatg gaattgtcta gcttccaact catgggaatc acagatttcc
     6421 gccctcatat agcggtgatt accaatatct atgaagcaca tattgattat catggtacaa
     6481 gaaaagaata tgtcaaagct aaatggaacc ttcaaaaaaa catgacagaa aaagattatc
     6541 tgatccttaa ctggaatcag tcagaactac aagaattagc gcaaagaaca aaagcacgcg
     6601 tactgccttt ctcgacaaaa gaagtgttgg aagacggtgt gtatgctgac gattactcaa
     6661 tatactataa aaaagaaaaa ataatggaaa tatctgaact tggcgtacca ggtaagcata
     6721 atgtagaaaa tgcactagct gctatttctg tagcaaaatt gtatggtata tcaaatgaag
     6781 ctatacgaga aacgttgcat tttttccatg gcgtaccaca tcgtacgcaa tatgtaggag
     6841 aaatccaagg cagaaaattt tataatgatt caaaagcaac caatatttta gcgactaaaa
     6901 tggctctaag tggatttgaa acgtctaagg tcgtgttatt ggctggcgga ttagacagag
     6961 gaaatacatt tgatgaatta attccttcat taaaaggaat caaagcaatg gtcgtatttg
     7021 gccaaacaaa agaaaagttg atggatgctg gaaaaaaagc aggcatcgag acaatagtaa
     7081 cagccgattc tgtagaacaa gcagttcctt tagcattaga aaacagtaca gatggtgatg
     7141 tagttttatt atcaccagcg aatgccagct gggatcaata cccaaatttt gaaactagag
     7201 gaaacagatt catggaagct gtgaaccgat taaaatagaa aaagtagaag cgagcgataa
     7261 aaaatgaaaa ttttggttac aggcggagga acagggggac atatttatcc agctcttgcc
     7321 tttgtcaatt atgtaaagac tcaagaaccg aatgctgaat tcatgtatgt gggagcaaaa
     7381 cgaggtctag aaaacaaaat cgtccctgat acaggaatgc ctttccatac cttggagatt
     7441 caaggattta aacgaaaaat ttccatgcat aacatcaaga cggtccgatt gtttcttaag
     7501 agtatccgtc aagcaaaaaa aatcctcagt gattttcaac cagatatcgt tatcggtact
     7561 ggaggatatg tttccggagc ggtagtgtac gctgcagcca aaatgggtat tccaacagtt
     7621 atccatgaac aaaacagtgt accaggtgtt accaataaat ttttaagccg atatgtggat
     7681 cggatcgctc tttcatttaa agatgcggct tccttttttc ctgaaaacaa agcagttctt
     7741 gtaggtaatc ctcgagcaca agaagtagca gatactaaaa aatcagatat tttgaaaaca
     7801 tttgatttgg atccagaaaa gaaaacagtc ttgatttttg gcggtagtca aggagcactt
     7861 aaaatcaatc aagcagtatc agttttttta agtgaatata ttccttcttc ttaccaagtt
     7921 ttgtatgctt caggagatag atattatgaa gagatcctaa ataaaataaa tcctgaaata
     7981 caaaatatta gtgtccagcc ttatatcaaa aatatggcag aggtcatggc ttccagtgat
     8041 ctgctggtgg ggcgtgctgg tgcaacttcg atcgcagaat tcacagctct ggggttaccg
     8101 gcagtgttgg ttcccagccc atatgttacg aatgatcatc agactaaaaa tgcaatgagt
     8161 cttgttcacg caggagcagc aaaaatgatt gcagataatg aactaacagg tgaatcacta
     8221 agtcagacag tcaacgaaat catgggtgat gaagaattgc aaaagcaaat gtgccgagct
     8281 tctaaagaac aaggaatccc tgatgcttca aaacgtcttt atgacttagt aaaacaaata
     8341 atcaaaaaat aaaggcggtg gtcttagtga gtaagaaaaa agaaccaaaa aaattgtctc
     8401 atccgtcttc tgatgaaaac aatttgactc cttggcaaaa ggctaaccga aaatatctgg
     8461 cagaacaaga gatgaaaaac caagagccaa aaaaagaaga cataacatta gatacacccc
     8521 tttcagattc tgaagaagaa aaaacggttc aagaggaaca gcaagaagaa agccagtccg
     8581 agaaaaaaat cgaatttttt gaagagatca agcaaaagaa aaacggtgga ccatataacg
     8641 gttctttttt gaaccggctg cccaatttaa aaagtcaacg gcacaaagta ctatttcgtc
     8701 ggttagcttt gatcatcact gtattgggta taccgctgat ttttttgatc tattatgttt
     8761 ctccttatag caaattacaa gcagttgcag tatcaggcaa taagacagtc aacagccaag
     8821 aaatcatctc tgatacaaaa ttgtcattag gagaaaatgt ttggggacag tattttcatc
     8881 gatctactta tatcgatcgc cttaaaaaag cacagccaag aatcgaaaca gcaaatatac
     8941 atttcaaagg gatgaatgaa ttcgatttgg atgtaacaga atataaagag attgctttga
     9001 tcgcaaaaaa caatcaatac tacccagtga ttgaaaatgg aacagtatta gatgaaaaag
     9061 tagcaaatcc tacaaaaaat ctccctatct tggaagagtt caaagactct gcaaaaatca
     9121 aagaattaac aaagcaatac aatcagttat catcagaatt acaaaaagca atttctgaaa
     9181 tcaagtacac gccaagagca agcaataaaa atctgatcca attgaatatg aatgacggca
     9241 atcaagttat tgtaaatatc aataatcttg cgaaccaaat gaaatattat tcgcaagtag
     9301 ccaaagatat ggatgaaaaa ggtgtgatcg acatggaagt tggtatcttc tcgcatcctt
     9361 acggtagcac tcaagaaacg gaagaatcgg gccaatcgga agaaacaact gaaagtgaag
     9421 aaaatgcgga tcaaacaaac gaatcaagtg aagaggcaga aacgcaaaaa gcacaaacac
     9481 ctggatcgtc tagcacaaac ggatcatctg attcaaatga ttcaagtgaa acaagtcaaa
     9541 tagaagataa tccaagtaca actccgtcaa gtttaaatga aaattgataa aacaacttta
     9601 aaaagtaaga atgtactttc taaaaaccag taatttatgg tagaattgga agcagtgaat
     9661 atatctatta ataagtatat tttaaaacgg acgctagttg ttcgcttgta tatggaatag
     9721 taagtaggag ggaatcccat tcatggcaaa aacaggaatg tatgtaggcc ttgatattgg
     9781 aacgacatct gttaaagttg tcgtggctga atatatcgac agccaaatga atattattgg
     9841 agtaggaaac gcaaaatcag aagggattaa ccgaggcatt atcgttgata tcgataaaac
     9901 ggtccaagcg atacaacgag cagtacgaca agcagaagaa aaagcaggta tccagatcaa
     9961 aggcgtaagt gtcggtttgc cagcaaacat gcttgaagta gaaaattgcc agggaatgat
    10021 tgcagttaac ggagattcaa aggaaatcac cgatgaagat gtcagaaatg tggcttcagc
    10081 tgcccttgtt cgttcaatac ctcctgaacg tcaaatcgtg tcaatattgc cacaagattt
    10141 tactgtcgat gggttcgagg gaatcaaaga tcctcgtgga atgatcggcg taagattaga
    10201 aatgtacggt ctgttgttta caggacctaa aacgatcatc cataacatac gtaaatgcgt
    10261 ggaaaatgcc ggattaatcg tcaacgaaat ggtgattact cctttagcgc tgactgaatc
    10321 aattctttct gacggagaaa aagattttgg tacgatcgtc attgatatgg gtggcggcca
    10381 aacaacaacg gctgttatgc atgataagca attgaagttt accaatcttg accaagaagg
    10441 tggcgaattt gtcactaaag atatctctat tgtattaaat acgtcattta acaatgcaga
    10501 agcgttgaaa ataaactacg gggatgcata cccagaacgg acatcaccag atgaagaatt
    10561 tccagtagat gtgattggtc agtcagaacc agtgaaagtc gatgaacgct acttgtcaga
    10621 aattatttca gctcgaatgg aacagatttt caacaaagca aaagatgcat tggaccaaat
    10681 cgaagcatta gagttgccag gtggcgtcgt attgacaggc ggagctgcta gtcttcctgg
    10741 tgttgtggac ttggcacaag aaatcttcgg cgtaaatgta aaattgtatg ttccaaatca
    10801 catggggctg agaaacccgg tatttacaaa tgtaataagt atcgtggatt attcagcaaa
    10861 cttgagtgaa gtgtatcaat tggcaaaaat tgctgttacg ggtgaaacct cagcagctcg
    10921 tcaaatggta gttgaacaag aaacaacaaa cacatatgag agctatgaag cacctgaaga
    10981 aacaatctac gatgagccgg aaccaaaaga gtccggagaa aatgtaaaaa ataaaatcaa
    11041 gggtttcttt acaaatattt ttgattaatt catcctggga ggaataagat atggaattct
    11101 caattgataa taatatcaat gacggcgcag taatcaaagt catcggagtc ggtggtggcg
    11161 gtggaaatgc tgtaaaccga atgattgaag aaaacgttaa aggtgttgaa ttcattacag
    11221 ccaacacaga cgtgcaagca ttaaaaaatt caaaagctga aacagttatc caattaggcc
    11281 ccaaatatac tcgtggttta ggtgctggtt ctcagccaga agttggacaa aaagcggcag
    11341 aagaaagtga acaatcctta cgcgaagctt tagacggtgc agatatgatt ttcatcactg
    11401 caggaatggg tggaggaacc ggtacaggtg ctgcaccaat cgttgcaggt atcgcgaaag
    11461 aattgggcgc attgactgtt ggtgtcgtga cacgtccatt cacttttgaa ggaccgaaac
    11521 gtggccgttt tgctgcagaa ggtatcgctc gtttgaaaga aaatgtggat acattattaa
    11581 ttatctcaaa taaccgttta ttagaagttg tagataaaaa gacaccgatg cttgaagctt
    11641 tccgtgaagc agataatgtt ttacgacaag gtgtacaagg tatctctgat ttgatcacag
    11701 cgccaggtta cgttaacctt gactttgctg acgtgaaaac agtgatggaa aaccaaggta
    11761 cagcattgat ggggatcggt gtggctagtg gagaagatcg cgtagtagaa gcaacgaaaa
    11821 aagcaatctc ctctccattg cttgaaacat ctattgatgg agctgaacaa gtacttctta
    11881 acatcacagg cggtttggat atgacattgt tcgaagcaca agatgcttca gatatcgtag
    11941 caaatgcagc aactggcgat gtaaatatca ttctaggtac atctatcaat gaagaaatgg
    12001 gcgatgaaat ccgtgtgacg gtcatcgcaa caggtatcga tgaatcaaaa aaggaacgca
    12061 agtctagccg accagctaga caagcacaaa tgcaatcacc tgctcaaaag actgtattag
    12121 atatggatca agctaaacca acttcttcag aagaagaaaa cagttttggc gattgggata
    12181 tcagacgtga acaaaatgtt cgtcctagag ttgatgattc caattttgac aatatcgaaa
    12241 aaaaagaatt tgatacattc aaccgcgaag aaacaaaatc tcatggcgat gatgaattaa
    12301 atacgccacc gtttttccgt cgtaaaaaat aaaggagagg aagagcacat gattgctgac
    12361 aacttgcaga aaatcaagca agaaattcag gattcgtgtg ctcttgttca tcgtgatccg
    12421 aaagaagtta cattagtagc tgtaacaaaa tctgttgaat ccgctcaagc tgaagaattg
    12481 gttagagctg gcgttatgaa ttgtgcagaa aaccgagtag ataaattatt agaaaagaaa
    12541 caagatttat ttgattttcc tgaaataaag tggcatttaa ttggtaattt acagcgcaga
    12601 aaggtaaaat tgattgtaaa tgaaattgat tactttcatg cgttggacag tttaagatta
    12661 gcagaagaaa tccagaagcg gttagagaaa gaattatcct gttttattga agtaaatgtt
    12721 tctggtgagg aatcaaaaca tggaatttca ccagatgagc ttttaccgtt tgtggaatct
    12781 ttagctgcct gcacaaaaat caaggttgtt gggttgatga ctatggcgcc taaagaagct
    12841 tctgatgagc aaatccataa agtgtttggt acgctgaaag aattgcgtga tcttgttcaa
    12901 gcaaaaggtt atgcccatgc accatgtagt gaattaagta tggggatgag tcaagacttt
    12961 caaattgcga ttgaagaagg tgcaacattt atccgtgtag gaacagcgct attcaaagac
    13021 gaataaagga ggaacaaaaa tgccgctttt aaacagagga gcaattgcta attttttcgg
    13081 attagctgat gaggaagagt acgaatacca agattatcct gaaacaacaa aaaaggctgt
    13141 acagccaact aaaaaaaata caacacagaa tcaatcaact cattctgtat ctgctgcgaa
    13201 tactcatgaa aaacaaaaaa cggcgacttc acgtcctgta acgcatgaaa gtattcagcc
    13261 ggctgaaaag aaaaaagtaa ttgaactaca tcaatcacct acaaaaactg tagcgaaaga
    13321 aagaacagct tcaagaacct ctacacaacc taaagcaaat cttgctggaa aaatcactgt
    13381 aatggaacca agagcttatt ctgaagctat ggcgattgct aaacgaatca ttgctggaga
    13441 agcaacctta gtgaattttc atttagtaga agaaaaacaa gcgagaagaa tcgttgattt
    13501 tctaacagga acagtttatg cactagatgg agatatcaaa cgtgttggag atgagatttt
    13561 tctttgtaca ccgactggga tggagattga cagttcaacc gctcaatctt ttgcagatgc
    13621 gaatttgttt gatctgtaag aggaggaact acgatttata ctattgcact attacttcat
    13681 ttacttcaac gggcagccta tttctatagc attatcttga tggtatatgc attgctctct
    13741 tggtttccag gcgggtatca atcagggcta ggacgattat tagctaagat ctgcgaaccg
    13801 tatatcagtt tgttcgatcg tctgcctttg aggattggac ccttggattt cacaatttta
    13861 ggggcaatcc tgatcttgaa cttagccgta caaggtttag cacagatcgt tatatggata
    13921 actgtccatt tagtttattg attgaagcaa caaaatcttt attctcagaa tagataggag
    13981 gggaaaattt tggatgcaaa tgtataccag cactttcggg cagatgaacg tacatttatc
    14041 gattcggtag gcgattggat tgaacaggtt caaggacaat atgcacccta tctgaccgaa
    14101 tttcttgacc caagacaatc ctatatttta gaaacactga ttcgacaaag cagtgatttg
    14161 cgtttcatgt tttatggcgg atatgaacag gcagaaagaa aaagatgttt gattttcccc
    14221 gattattacg aacctgtaga ggaagatttt gaaacagcat tgtatgaaat ccattatcct
    14281 agcaaatttg ccagcctaag tcatgggaaa atccttggaa ctttagtggg aacggggatc
    14341 aaacgctctt actttggcga tatcatctcg gacggtgatc gctggcaagt atttattgca
    14401 aatgaggtgg atcattttgt tgctgcacaa gtaacaaaga ttggaaaggt tgctgtacgc
    14461 ttggaaccaa tcaaatacac agaaatgatt atgccgaaag atagctggac acaagaaaga
    14521 ggaacagtta gttccatgcg actagacagt gtgatctctg agatttataa tatttccaga
    14581 cagcggtcca agcagctgat cgaagctggt aaagtaaaag tgaactggac agaaaataca
    14641 agacctgatt ttgtacttga actattagat attatttcag tcagaggtta tggcagagtc
    14701 cagatccaag aacttgaggg gaagacgaaa aaagaaaagt acagggtact gttcggtgta
    14761 ttacgtaaat aaaataaaaa ggtggtttac tcatggcttt aactccatta gatatccaaa
    14821 ataagacatt cccgacaaaa atgcggggat ataatcaaga cgaagtagat gatttcttag
    14881 atttagttgt tcgtgactat gaggaattga ctcaaagaaa tcgagaatta gaaaaagcag
    14941 tcaaacattc tgaagaaaaa cttgagtact tcaatgaatt aaaagatgct ttgaaccaat
    15001 caatcatcgt agctcaagat acggctgata aagtaaaaac aagtgcaagc aaggaatccg
    15061 aagtgatcgt tacttctgca caaaacaaag cagatgagtt agttgcgaac gctgaaaaac
    15121 gtgcacatca attaacaact gatgcagaag aaaaagcaag aaaaatcttg acagatgcaa
    15181 cagaaaaagc aagacagctt gcgacagaaa ctgaagatct taagaaaaaa acacgagtat
    15241 tccatcaacg tatcagcttg atgcttgagt ctcaattaga acaagtgaaa agtccggaat
    15301 gggatgaaat tttacaacca ttctccagct acgtgactga cagccatgaa gtgatcaaag
    15361 aagttttagc aaaacagctt gacaatgaaa atgatactga tgtaaactca gacacaggag
    15421 tcatagaagc acctatgacg gcatttgata cacaggctat tgatatctcg atcgtgcctg
    15481 gtgaaaatga aaacgagtag aacagaaaag cactttgtta ttcttaaagc gagccgatga
    15541 gagtggaagt tcggtaagac actgcatggt gaaagatcct ctacttgaga cctgcttttc
    15601 cgaaattaga gtaagaagca gcggctgacc tcgttattgg tcacagagag tttgcactag
    15661 atagatgcaa ctaaatttgg gtggtaccac ggcatgttcg tcccttgcgg ctgaacatgc
    15721 ttttttgtgt ccggaaaaca aataaaggag tgtatggttg aatgaaaatg aaagaaacct
    15781 tgcaattagg aaaaacaaaa ttccctatgc gtggtaattt gccaaaccga gaaggcgagt
    15841 ggcaaaaaga atgggaagaa aatcgaatct accaaaaaag acaagagttg aacgaaggaa
    15901 aacctagctt tgttcttcat gatggacctc catatgccaa tggtaatatc catttaggac
    15961 attcattgaa taaaatcagt aaagacatta tcattcgttc aaaatcaatg tctggctatc
    16021 gtgcaccata tgttccagga tgggatacac atggattgcc gattgaacaa gtattggcta
    16081 ataaaggcgt gaaacgtaaa gaaatgtcca tggcagaata cttggaaaaa tgtcgtgaat
    16141 atgcactttc ccaagtagat aagcaacgag cagatttcaa acgcctagga gttgcaggag
    16201 attgggaaca tccttatgtt acattagatc cttcttatga agctgcacaa gtacgcgtat
    16261 ttggtaaaat ggctgaaaaa ggctatattt acaaaggatt gaaaccaatt tactggtctc
    16321 cgtcaagtga gtcttcttta gcggaagctg aaattgaata taaagatgta aaatctccat
    16381 ctatttttgt agctttcaat gtcaaagacg gcaaagatat tcttgatgaa gatacagctt
    16441 ttgtcatttg gacgacaaca ccatggacat tgccagcaaa ccaaggtatt gctgtaaatc
    16501 caaaatttac gtatgtattg gtggaagccg atggccgtaa gtttgtcgta gcgaaagatt
    16561 tgctagaaac agtccaatca gaaatcggtt gggaaaatgt aacagttcta aaagaatttg
    16621 ctggacaaga aatggaatat atgactgcaa gtcatccatt ctatgaacgt gaatcacttg
    16681 tgattttagg cgatcatgtt actttagacg ctggtactgg attagtccat actgctccag
    16741 gacatgggga agatgactat atcgctggta gcaaatataa actatctgta gtgtcacctg
    16801 tagactcaaa aggattgttt acagaagaag cacctggatt tgaaggtatc ttttatgaca
    16861 aagcaaatcc tatgatcact gatctattaa aagaaaaagg agctttgctg aagctagatt
    16921 tctttactca tagttatcct cacgattggc ggacgaaaaa gcctgtgatc tatcgggcaa
    16981 ctccacaatg gtttgcatct atcgataaat tccgtcagaa tattttggat gaagtagaaa
    17041 aagtagagtg ggttatccca tggggtaaaa cacgtttgta taatatgatc cgtgaccgcg
    17101 gcgattgggt aatttcccgt cagcgtgcat ggggagtacc acttccgatt ttctatgctg
    17161 aaaatggaga agcaatcatc acaccagaaa caatcgaaca tgtggcaaaa ttatttgctg
    17221 aacatggatc aattatctgg tttgaaaaag aagcgaaaga acttcttcct gaaggattta
    17281 cccacccagg ttctcctaat ggcgaattta caaaagaaaa agatatcatg gatgtctggt
    17341 ttgattctgg ttcttctcat gaagctgttt tacgtcagcg accagaatta tcattcccag
    17401 cagatatgta tttagaagga tcagaccaat atcgtggctg gttcaactca agtatcacga
    17461 ctagtgtggc tatcaatggt gtagcaccat acaaatcagt tatttctcaa ggatttgctt
    17521 tagatggtga aggccgtaag atgagtaaat ctttaggaaa catcattgca ccagaaaaag
    17581 tgatcaaaca atttggtgca gatatccttc gcttatgggt atctagtgta gattatgaag
    17641 cagatgttcg tgtttctatg gatatcttaa gtcaagtggc agaagtatat cgtaaaatca
    17701 gaaatacgat gcgtttctta ttagcgaata cagaagattt cgatcctaaa aaagatactg
    17761 tttcgtacaa tgatttacgt tctgtagaca aatatatgac cgttcgattg aaccaagtaa
    17821 tcaaagaaat ccgagaagaa gggtacgaca aatataactt catgcatatt taccgtacag
    17881 ttatgaactt cttgacggtt gatctatcgt cattctacct ggattttgct aaagatgtag
    17941 tttatattga agcagaagat gattatcaac gtcgttgcat gcaaactgtt ttctatcaaa
    18001 cagctgtagc tttgacaaaa ttattgactc ctatcattcc gcatacagca gaagaaatct
    18061 ggtcattcct gaaagaagaa gaggagtacg ttcagttaag cgagtttcct ggttatgaag
    18121 agtttgcaaa ccaagaagaa ttgatggata cctggactgc tttcatggac ttccgtgata
    18181 atgtcttaaa agcattggaa gaagcaagaa acagcaaatt gattggtaaa tctttggaag
    18241 caaaactaac tgtttatcca aaacaacaag ttcgtgaaat gttaaaagct ttggatgcag
    18301 atattgcaca attattgatc gtttcaccag actactttga agttatggca gcagatgaac
    18361 aagtgccaga taacgctatg gtatttgatg atgttgctat tacagtggaa aaagcagacg
    18421 gggaaacttg cgatcgctgt cgccaagttc gtaaagatgt aggagtagat gaaaaacttc
    18481 ctcatctatg cggtcgttgt gcaaaaatcg ttgaagactt ctatcctgaa gctgtagctg
    18541 aaggattcga agaaaagtaa aagggaaaag ggggtgtgac atgttctttg tcacaccccc
    18601 ttttgttatc aatcgtttaa ttttccacgt tcacgatacc agttatctgg ttgccagatc
    18661 cattcgaatc cgtctttttc aagtagttcg aaagctgctt taggtcccat tgatccagct
    18721 ttgtagttgg gaaaatccgg ttgtgtttgg tcccaagctt ttcgaatgac atccacgata
    18781 tgccaagatt gagcgacttc ttcccaatga gagaagttcg ttccatcacc attcaacgca
    18841 tctaatagta atttttcgta agcttctggg ctattttcaa ccatttctgc actatttcta
    18901 aattccaatt tgattggttc cgtttcaaat ccttgcccta cttctttccc attcaaggat
    18961 aaagaaaatc cttcagttgg ctgaatatag atcgttagaa cattaggtgg aagtgtcgta
    19021 tcgtcacatg gttcatcgac agatgttttg aatacattaa caggaacttg tttgaatacg
    19081 atgttgatcc gagtcccttt ttcagtcaaa cgtttgcctg tacgtacata gaaaggaaca
    19141 cctgaccaac ggaagttatc gatagtgaat ttgcctgcga cgaatgtttc agtctctgat
    19201 tgagcatcca ctttgtcttc ttcgcgatag cccttgaatt gtacaccatc caattcacct
    19261 tgagcatatt gcccgcgaac gaagttttct aacacttctt cttcgttata aatgcggata
    19321 gctttcaatg ctttgatctt ttctgtacgg atttcttttt cggaaaatgc tactggcggt
    19381 tccattgcaa gtaatgctac aacctgtaaa atatgatttt gcaccatgtc tttaagtgcg
    19441 ccactatgat cgtagtagcc cccgcgatct tctacaccga ggttttcgcc aaaagtaatt
    19501 tggatattgt cgatatagcg attgttccat tgagcctcaa aaatattatt ggcaaaacgg
    19561 atagctgaga tgttttggat catttctttt cctaaataat gatcaatcct gaaaatatct
    19621 tgttctggga atacttctct gatttgctgg ttcaattcaa aagcagactg gtaatcagaa
    19681 ccgaacggtt tttctatgat caaacgattg aagccatttt ttgtcataat attttgtgat
    19741 ttcaaatgag aaacgatcgt tccaaaaaat tgaggtgcca tagccaaata atagacacgg
    19801 ttaccgttta attgatagtg tccatctaat cggtcagaca attcttttaa tgtctgataa
    19861 tgcgttgaat catttacatc atgggattga tagtaaaaat ggcttgcaaa ggtagttgct
    19921 tcttcatctg aaggattcaa tgcacggatt gtttctttga caacttcacg gtagtgctca
    19981 tcgctccagg gacggcgagc ggttccgatt acagcaaaat gctcgcctaa atttcctttt
    20041 ctgtaaaggc ggaacaaaga cgggtacaat ttacgctggg ctaggtcacc tgtaccgccg
    20101 aatatcgtaa acaatacatt tttttcatta gacatgtgga tacactcctt tatattttat
    20161 cgacaaaaat cgtgctggcc gccttaaagc tgacggggat tttctttcta tcattttgga
    20221 tagtgatcgg tccttcataa gctgcgatgt caacgatctc atattcttcg ttgatattca
    20281 ggtcaatagt gactaagtaa tccaacaatt ctttttcatc aagcactcgt gcgatacgca
    20341 cttttgtacc gacaggatag gactccaatg tttgtcggcg ttcttcatgg acaacttcct
    20401 cttttttagg aatcgatcct ccatgaggac aatgttcagg atgattcaaa tagttttcta
    20461 aatgatctgc aagatgttcc gaagtcacat gttccaatac ttcagcatca tcatggactt
    20521 cattccagct atagtctaaa tgttcaacta aaaaaacttc ccataagcga tgtttgcgaa
    20581 tcagactgct agctttttgc agtccgcttg cagtcaattg tacaccttga taaggggaat
    20641 gttctacaag ctcttctttc acaagtttag aaatcatttc actgacagat gcagctgaga
    20701 catctagact agatacgatt tgtttattgt tgactttcgt tgtatctcca cctagttcta
    20761 aaatgatttt caaataatct tctcgatttg gtgtcatatt cgatcatcct ttcatggtaa
    20821 aaaaccatca ttagtaagtg taacaaaaaa aacgttaatt gactattttt ctaaataaaa
    20881 aacaaaggat ttttagacgt ttttacaaca aaaaacgaaa gatgatagag aagactcatt
    20941 ctttcgttta taaaggatat atatatacat tgggagggat ctatttttca gataacaact
    21001 tatatgaagt gtctgcagat tcgtcataaa gagtttgttc gggataagaa aagataagat
    21061 aaaaaccata ctgatcacgt acgactaaac gatattcatc tatttctacc acagatgtgc
    21121 caagagactg accgttgatc aaaaacgtgt aatcagatgt aatctgtaat tgttgttttt
    21181 gctggctatc actaaagaac catgtacctt gatactttga gagtccttct gacttttccg
    21241 cttgtttttt ctttttcaag taaataaaac ttgagagaac ggtaatgagt gaaccaagaa
    21301 aatagatcat tttttttatc accaaatcac tcctttatga gaaaataatc gcatagacat
    21361 attctttctt ctttatttca ttgtaccgaa taaaagctaa aatttctcta agactttcga
    21421 ctggtttttt tactgttttt ctacaaatta tgataggata catagtagga agaaaggtga
    21481 cataatttga agtatcagca agtggaaata gcttttttta ttgaccgccc gaatcggttt
    21541 atagcacatt gtatgaaaaa agacggagaa ataattattg cgcatgtcaa aaatacaggt
    21601 agaggaaaag aggtcctgat accaggcgct gaagtggcta ttgtatttgc tcctggacct
    21661 aaacggaaaa ccgcctatga tctgattgct gtaaaaaaga agggtgactg gtttaatatc
    21721 gatagtcaat tgcctaatcg gctggcaatt gatggtattt tagacggcac gatcctttta
    21781 ccaggattaa ctggtccatt tacggtatac aagcgtgaag taacttatct tcactccaaa
    21841 tttgatattt atttagagac ggtggtcgga caaaaagcat ttgttgaagt caaaggtatg
    21901 acacttgaaa ataaagcgat tggtgctttt cctgatgcgc ccacactcag aggattgaaa
    21961 catataggag aattaacgta tgccgcacaa gatggttatg ccgcttatgt cttgtttatt
    22021 gctcagttcg aacatcttca tctggcaacg atccatgaag aaatgcaacc agcactagct
    22081 gatatggttc gacatgcgca gcaaagcgga gttcaaatcc tcgcctataa ttgtcaagta
    22141 tcggcagatg aggtaactgt caagcaggcc attccatttg atgtagatat cccttttgaa
    22201 gatcctatac aatagaaaga aggtaaaata atgatcaact taggtattat cggaacaaat
    22261 tggattactc atcaatttgt ccaagcagct ttaagcacaa aaaaatatga attgacagcg
    22321 gtatattcta gacatttgga aaaagcacag gaatttggtt ctcattatga aggggatatc
    22381 gaattcgcaa cagatttgaa gacttttttc aatattactc atatggacac catttatatt
    22441 gcttctccga atagtcttca ctttagtcaa gctaagcaag caattttggc aggaaaaaat
    22501 gtgatagttg aaaagccagc tttttcgacg ccaaaagaaa tggatgaaat catccatttg
    22561 gcaaatgaac acaatgtgta tttttttgaa gcagcaagaa atatccatga atcaggtttt
    22621 aaaaagattg ccgattactt accattaaaa gacgagataa tcggtgcaaa tttcagctat
    22681 atgaaatatt cttcgcgcta tgaccaagta ttagatggag aagaaccgaa tatcttctcg
    22741 actcatttct caggtggtgc attgatggat ttaggtgttt accttgtata tgcagctgtt
    22801 gcttggtttg gaatgccaaa agaagtccat tatttcccaa gaaagatttc tactggtgta
    22861 gatggtcttg gatgggggat cttacgttat gagaattttg atgtatcgat ccagccaggt
    22921 aaaatcggtg attcttattt accgtcagag atttattttg actcaggaac gctagtgatg
    22981 aatggagtca atgcaattga acaagcacaa tttcatgatc gcgcgcataa agaaatcatt
    23041 gatttggcgg taacagctgc agaaaatcca atggcagatg aagcagagga ttttgccaat
    23101 gtcatggctc atccgaaaga tccagcttgg ggattgttgt atgaagagtg gatcgagcta
    23161 gctagaaatg tgaaccaagt gatttatgat ttaagaaaaa atggcggcat caaattcgat
    23221 gcagataatg aggaagattt ttaatgaacg aatttgtaca agtatttccc gaaaattggc
    23281 agaaaaaata tgaggataaa gggtttatct caccttctct tattcaacga gcagtttttc
    23341 agccactaag taataaacaa agtatagtag cggtttctcc tacaggttca ggtaaaacac
    23401 tggcttatct ttggcctcta ctactaaacg tggaaccagg tgaagcaagt gcgttggtca
    23461 tttttgcttc ttctcaagaa ctagctattc aagtagcaga tgttgcacgt gaatggggaa
    23521 aagacaaaga gttgaaagtc caatctttgg tgggcggagc caacgtcaaa cgtcagattg
    23581 agggtttgaa ggaaaaacca gaaatcttga ttggtacacc aggaagaata ttggaattga
    23641 tgaaagcaaa aaaaataaaa gcacatcaag tgaaaacaat ggtatttgat gaagcagacc
    23701 agctatttga cgctgggaac agccaaatca ttgatcaaat tcttcatcaa gctccaacag
    23761 agtatcagct agcttttttt tcagcgacag cagatcgttc ccttgaaagc attgaaaaaa
    23821 tcactgggaa aacgtttgag acaattgacg tgacagcaga ggacgattct cgaaaaggac
    23881 tgagacatta ctttttacgt gtaccgatac gaaagaaaga tgactacttg agacgtctca
    23941 cgcatataga agattttcat ggtctgattt tctttaatca actgactgaa ttaggcgtaa
    24001 tggaagaaaa attgctgtat agaggtgtac cggtaggaag tctggcctct gatcagaaca
    24061 agctactccg aaaagcagcg ctagaacaat tcaaaaagaa aatggtcaga gcacttttga
    24121 ccacagatgt ggcggctaga ggtttagata ttacaggtct tccttatgta gtgaatgcag
    24181 aagttccgtt aagtgaagaa gcttatttgc atcgttcggg aagaactggt cgaatgggta
    24241 atgaaggaat agtaattaca ctcgtacaag ataataatct aagagattta aaacggcttt
    24301 tacgtccatc aaatataaca cttgaagaga tttttttata cggaggtaag ctgcaaactg
    24361 agcagccaac caaagaagat gcagctcctg cagtaaataa aaagtatgcg aaaacttttt
    24421 ctggaaagga gcagaaccca gaatataaca aagaaaaaat aagaaaaaaa gttaaaaata
    24481 aatcaaaaaa agagagaaat aaaggagcaa ggcgcaaata agcgcctttt tatgctatga
    24541 ttatgaaaac gggaacaaaa ttggttgcca gaggaggggt tatgtgaaag aacgtcaaga
    24601 agagatcttg cagctattga cagaaaacca acaaggattg acagcaagtg atgtagctga
    24661 gcgcttaact atcgatcgaa gcaatgcaag ccgatattta agtgaactat acaaggccca
    24721 tcatattgta aaaaccgctg gccgaccagt cgtttactct ttgccaacgg aaaagtcaaa
    24781 atctgatgaa gtgcatgtcg actcttctac gcaagtgact tttgaaacac tcgttggaga
    24841 aaatgattct ttaaaagtca gcatacagca ggcaaaagct gcgattcttt atccaccgcg
    24901 ggggttgcat acgattattt ttggagaaac tggtacaggg aagtcgatgt ttgctgaatg
    24961 tatgtatcat tttgcaatcg attcagagat gctttctgca gatgcgccat ttgtttcttt
    25021 caattgtgcg gattatgctc aaaatccgca actgctcttt ggacacatct ttgggatcaa
    25081 aaaaggggct tacacaggag cagctcaaga tagtcccggt ctaatagcca aagcagacgg
    25141 cggtatattg tttttagatg agatccaccg tttaccgccg gaagggcagg aaatgctgtt
    25201 tactttcatt gataaaggta tttaccgtcc gcttggagaa agtgcacaag tacatgaagc
    25261 atctgttcag atcattgggg cgacgactga atcttcagaa agtttcttat ctacgttcaa
    25321 tcgacggatt cctatggcaa tcaccttgcc gaatcttgct tcacgttcac tggatgagcg
    25381 ttatgaaatc atctctcttt ttatcaaaca agaagcgaat cgtttgaatc aacgtatcga
    25441 tatcgaaaaa gaagcgattc ttgccttcat gctttatgat gcggaaggaa atatcggtca
    25501 agtgaaacga gacttgaaac tagtctgtgc aaaatctttt ttacattacc gtactcatca
    25561 tgagaaaaag ctaatcattc gaaaagaaga actttccctt caagtacaaa aaggattact
    25621 gaaaatcaag gaaataccgg aacgtctcga ccgattcatg gacagcaaaa gtcagtattt
    25681 gacatttgaa ccaggatttg ctgatgttgt ctggtcacaa gatccagaac gaaacatgca
    25741 ggtctataac gatattgagg aaaaagtcac ttctctttcc gaaaccggtg tggagaacat
    25801 tgatctagag accctcattt caaaagatgt ggatgcttat ttccagacat atgtcaaaga
    25861 attgactaaa agttcgattc ccaaagatct tttgccagat gaaatctggc ggttaaccaa
    25921 tcaattgtat gatgtggctg aaaaacggtt agaccgtatt tataatgaaa aagctcgttt
    25981 tgcttttgct ttgcatctgc aaagtacatt ggatcgtgtg agagaaggtc atatgatcgt
    26041 ccacccagat ttgaataacg tacggaaaaa ttttaagagg gaattccagg tagcaatcga
    26101 tctttcaacg atcattgaag aagaagaaca agttgagatt ccttttgatg aaatcggctt
    26161 tattagtatg tttctttcta tcaatctagg agaaagtgag cctgttaaag aaaacaaagt
    26221 agatgttctc atattgatgc acggcagaca gactgcttca agcatgttgg agatggcaca
    26281 agaactatta ggaacaaaag tagggaaagc ttttaatatg ccactggtta tggaagttca
    26341 aacaatgtat gaagaagtgt tacaatatgt caaaaactat caagagcatt tgacgaatgg
    26401 cctactcttg ttaacagata tgggatcatt aaacaccttc agtgatttga tttatgaaga
    26461 gactggcatt cggacgaaag ctatcacgat gacaagtacc atgattgtga tagaagcgat
    26521 acggatggca agtgttggaa gaagcttaga agatatttat caaaatatcc agctttcctt
    26581 tgaatcgatc gtacgtgatc agttcaaagc ggaaaagtct gaaactttac agcgtaaaaa
    26641 agccgtaatc gtgacatgct ttactggtga aggagtagct gccaaattat atcagcgtat
    26701 tatgccagtg attaatcaaa ataaggtaga actgatccag atgcagtttt tagaacgtga
    26761 atcattcaaa aaacacatcg atgccttatt ggaagaatat gagatcaaag caattgcggg
    26821 aacagttgat gttgattatc aaaatatccc atttttttca gcctatgacg tttttgatga
    26881 tgaaaaacta aatattttaa agcgtatagc tgatgatgag atcccgacag ctaaaattgt
    26941 cgattcactt gctggatcat taagcgatgt cttttctatt gatgaactga tccgttcatt
    27001 gcaaaagaca gttcagcaga tccagaccga tttgtcgatt atcgtggaac ctggagtaga
    27061 tactggaatc gttatccatt tagccttcat gttagacgga ttgatcaaac aagaaagcca
    27121 tcggcaattt gagaaactgt ctattttctc aaaaatttat cgtttggaaa tggatatcgt
    27181 tcgggcaaac ttgatggcac tagaacgaaa atatcaggta aaaattccag aagatgatat
    27241 cgcatttctt acacaaatgt ttcttgaaaa taaaataaaa aaatgaacac actaatcata
    27301 ctgtttacac actaaacatc tgtgtgcgca ctataaaaaa gagctaaaaa gcatgaaaac
    27361 gctatttcag ctcttttttt tattggcatg cttcttgcgt tatataaaag tgtaaaaaaa
    27421 taaaaggagt gttaaaaatg gcaaaaaaaa caatcatgtt agtttgttca gcagggatga
    27481 gtacgagttt attggtcaca aaaatgcaaa aagcagcaga agaaaaaggg atggaagccg
    27541 atatttttgc ggtctctgcc tcagatgcag ataataattt agaatctaag aatgtcgatg
    27601 tattattatt aggtcctcaa gtacgtttca tgaaagctca atttgaacaa aaacttgcgc
    27661 ctaaaggtat tccattagat gtcatcaata tgcaagacta tggcatgatg aacggagaaa
    27721 aagttcttgc acaagcagaa aatctaatga aataaatcaa gtatgcagtt gggggtataa
    27781 caacgtggaa gatcaacaaa atctagaagc catcatgggg ttaatcatgt ttggcggtaa
    27841 tgcaaagagc gatgcaatgg aagcgattgc ggctgctaaa atcggtgatt ttgagttagc
    27901 ggataggaaa atcgctgatg cagaagaatc acttatacaa gcgcatcatg cacaaacaga
    27961 catgttgaca caagaagcac aaggaaatca tatgcaagtg acattattga ctgttcacag
    28021 tcaagatcac ttaatgacat ctattgcgtt tactgattta gcaaaagaaa tcatcgaact
    28081 ttatcgtcgc gtatacagcg aataaataca acttcttttt tgaacggaag aagaaaaatt
    28141 tttgaacgga ttttttattt tgttataagg agaactctct ttatataaaa aacttatagg
    28201 aggaagtaaa aaatgaacgg actatcaagt tggatggaga aatacatcct gccggttgcc
    28261 gcaaaaatcg gagcgcaaaa acatttagtt gctttgcgtg atgcttttat cggcatactg
    28321 cccgcaacaa tggcgggatc agtcgctgta atgatcaatg caattattcg tgatttaccg
    28381 caacagttta acagtaccta tgctgacagt ttagcagcag acaaaggaat ctttgccgta
    28441 ttgaatgtca tcatcggaat caatggtttt gtctggaacg gtacactagc aatcgccgga
    28501 ttgatctttg ccttttcttg ggggtataat cttgcccgag cttatggagt aaatgatcta
    28561 gctgggggga tcgttggttt agctaccttg attcaaggta tttcctttgg tccagcatta
    28621 tcagcagcat tagatgtgaa tgttccttca aatgtcgcag atgcaatcaa tagttctgag
    28681 attggtgcaa cgattgcaga tggcacactg tcagttggat tatggggttg gctaaaacta
    28741 gatcatttga atggaaacgc ttattttaca gttatgatca tgggagctat ttcagtcatt
    28801 attttttgta aattgatgct tgccaacatt acgatcaaaa tgcctgattc agtaccacca
    28861 gcagtatcta aagcatttgc agccatttta ccagcgacga tcgctttata cgtcattgct
    28921 attatcaatt atatcgtagg tcgtgtagct gatgggcaac tgatcattga tttggtgcaa
    28981 aaatatattg cagaaccatt cctaggatta tctcaagggt tgggagcagt attgatcgtc
    29041 acagtatttg ttcaagtatt ctggttcttt ggtattcatg gatcaaatgt attggcacca
    29101 gtgctagagg gaatctgggg acaagcacaa ctaatcaata ttgatatttt ccaaaaaggt
    29161 tataacggaa aaactggtac accagccgtg ttagatgcaa tcgaagatgg aaaagcttat
    29221 atgtgggtcc gcggatcgtt cgatgctttt gcatggtttg gcggatcagg cggaacgatc
    29281 gtcttactga tagctatctt gattttctct aaacgtgcag attatctaac agtagctaaa
    29341 ttatccattg ggcctggtat cttcaatatc aacgaaccag tgatgtttgg tctgccaatc
    29401 gtattgaatg caatcatgtt tatcccattc ttgattgctc cagtagtagc aacagcaatt
    29461 ggatggacag ctacttatct tggcctagtc gctccggtat cgcagcaagt cacatgggta
    29521 gtaccgccgt tcttattgtc attcttagca acaggagcgg attggcgggc accgatcgta
    29581 acattagtat gtatggtcgt gactttcttg atttgggcac catttgtcat ctctgcaaac
    29641 aaaatggacc ctgccgcagg cgaacaacaa taagatgaac gtgaggagcg ggacgcttta
    29701 agtttcgttc ctcataatga attttcgagg aggaactgaa atgattacaa ttgccagtgc
    29761 aatgcaacaa gatgaaatca aagaattgtt agaaggatac aatgaagaag gaatgacttt
    29821 tacatttaaa gaaaaacaag gaatcaagct gttctttgaa acaagtgcag aagatccgga
    29881 agttgcggct aaaaaagcaa aggaactaat caaagcacag ccttggggaa cagtccttta
    29941 ttttcaatca acagctgttt agaaaatggt ttaaaaagat aggatggttg agatgataaa
    30001 aaacggttta tttttatgta gtattggttc tttgatcgtg ttgtatagtt tgaatccaca
    30061 ttcgcaaagg tatgatttgt atagtatggt cacaggtctt tttctaacag gattaggaat
    30121 ctatttcttc atgaagggga aaaaaagaga agaagcaaag gagaaaaata actaatggaa
    30181 atcacgaaaa aattaaatat tccagcatcg tttttttacg ataaagttat ggactctgtc
    30241 cttttcgatg tacgtaaagc gaccggcaaa tcagtaacta gaaaacagct gaaaaatttg
    30301 gagtacgtca aacaattctc gcaaaataac cgggctcgca taaaaatcga agaagtcatt
    30361 gaaaaccagt cttataagtt tcgtacatcg acaacgaaga atgaattcgt cgttcattat
    30421 caaatcaaac cattggacga ttcgacctgt gaggttcatt acacagaaaa aatggagtct
    30481 tatggcatgc ttcaaaagtt gaatgatatg cttttaggaa caatcttgat gtattttaaa
    30541 cggagacgtt tcaaaaaaat gctagatatg atcgaagcaa cgtattaaat caatgaaaca
    30601 tggtactatt tagttattga aaaatgaagt aagctagaag gaatttttca taggaagata
    30661 gatctttcct gcgaatcctc actagctttt ttgtaggaag gggcagcaaa atgacaaaca
    30721 aagtaagaga gctaggtgta tcttcgattg aggaaatgtt tgatttagcc acttatgcat
    30781 ttaatggggc agatacacct gaaaggcgag aacgttttcg tacacttgcg gagaattctt
    30841 ggaattacgg attttttgat gaggaggatc ggctaacaag tcaagtcatg gcgacccctt
    30901 ttccagtgaa tttttacgga caagagtacc taatggccgg cattggctat gttgcttctt
    30961 atccagaagc aaggggacaa ggcgggatca atcagatcat ggaaaagatc cttgaagatt
    31021 gccgtgatcg taaagtaagc ttatcttatc tggcgccatt ctcctatccc ttctatcggc
    31081 gctatggcta tgaacaatca tttgataaaa tcagttatca actaaacagt cgtgatgtgc
    31141 cgtttatcaa aaaaaaatct ggaaaaataa agcgtatgga ttttgaagat gcaaaagctg
    31201 caatcagaag aatttatgaa cagatgccgg aaaaccaacg agctggtctg aagcgcgaag
    31261 actggtggta tacgtataaa ttcaagcaaa aaggcaatta ccagtttgct ttgcattttg
    31321 atgataatga ggaagcaacg ggatatatcg tttatcagct tgcaacgtct tcctttatta
    31381 ttgcggagtg gggatttttg gatcatgatg ctttttgcag ccttgttcgc ttcgtaagtt
    31441 ctcacaatgg tgcttttgaa acatttgaat attcatgcgg ctacggcggt aatgatcaaa
    31501 attatctatt agaaaatcca tttgcttctg taatgatcac accttatatg atggcgagga
    31561 tcgtagatat ttctttgttt ttggaaaatt atccatttaa aaagagacaa gctgcatttg
    31621 ccctacaaat agaagaagat cattatgcga aatggaatga aggaatcgtg gaagtaacaa
    31681 tcgaaaatgg aaaaaatcat ctctcaaaag tagaacaaac aaaactccca atcgttcgag
    31741 gaagtatcca acatatcacg cagctattat tagggtatcg taaagtggaa gagttggtat
    31801 tccaagaaag aatacaggtg acaaaagaat atgaagaaac gttaagctca atcttgccac
    31861 agcaacagcc aattcttaat gactattttt gataaaaaac gggtgatgac aatatttttg
    31921 tcaccaccct tttgtttttc atttggcatt tgaaggaaaa tgttaaagta aatcctcttt
    31981 tttcgtaaat ttgaattttt tgttttcttt tgttttaggg ttttcttttt tgtggtcttg
    32041 gattgcttct ttcgtaatct tttcccgatt ttttttagat gaatctaagc gttctgcgta
    32101 tactttttct tttggcttat cattcattgg aaaggacctc cctacattat ttcattctac
    32161 ctctagttta tcatctgtta aggactagtg gaaacgtttt gcctaactta taagctgatt
    32221 tttttagcag ataagcttgc aagtaaaaaa caaacaagct acaattgatt tattgaaatt
    32281 gttcctgtag ctcagtagga tagagcaatc gcctcctaag cgataggtcg ctggttcaat
    32341 tccagtcagg aacgtataaa taacccctgt atcctttgcg gtacaggggt ttgttttgta
    32401 tttgtgccaa aaatgtgcca aactagttat catttttata taaactatcg aacacagtat
    32461 tgattttttc tttatcctga tcttcaagct ctcgaacaat atgaagataa gtagacattg
    32521 tagtttctaa actgctatgt cctaaacgtt tcgatacgct aagtatattt actccttgat
    32581 aaagtaaaat agatgcatgt gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc
    32641 ctaattcttt taattttttg cgcaaatatt tgctgacggc gtttgatgaa actaatccat
    32701 tttttaagtt gaaaaaaaca aaattatgag ggtttttgat ttcaaaattc tcgaataatt
    32761 ctttttgatt gattttaaac ttttttaaga gatcaatcgt atgtttatca atggaaattt
    32821 tcctatttga agtttcgttt tttgtttttc cccattcatt taatttataa ttccatgttt
    32881 tattaatagt gatgatttga tcttcgaaat cgatatcttc ccatgttaat cctagaagtt
    32941 cagcaaatcg cattccagtg gcaccagcaa ctaacacgag cataggagaa gagtactgcg
    33001 cagaaaggtt ttcttctacg agtttcatta aagatttaaa ttgatcataa tctaaatatt
    33061 tatcttcttt tggcttcaat gaatcttttc ctttgattac tgcttttcta gttggatcaa
    33121 acggaattaa tccttcttct acggcatctt tcaacgacgc tctaatatgg ttattaaact
    33181 taataacggt agattttaca tggtctttag catacttatt taaaaagcgt tgatatccca
    33241 ctctatctaa atcagaaatc aataccgctg gcatatattt ctttatgttc attaacgtat
    33301 cttcgtattt tctataagtg ataggagata ctgttccttc tttataaagt tgcatccaat
    33361 cctcaaagta atctgataat aatagatttt tgcgatcccc tttaaggcct ttgttcaagt
    33421 tatattctaa ttcgttggcg gcatcttttg cttctccttt tgtcttaaat cctgattttc
    33481 ttatcttcgt gtattttccg tcatcttttt tgtatgaaat ttcgtattgc caactatttc
    33541 cacgtttgac taatcttgcc ataattgaat cagctctctt tctctgatac aataggaact
    33601 ataaagaagc ccattgtttg ggttttatta ttttttgcac atcctttttg cttgccggcg
    33661 caaggatgtg ctatttgtcg ttttaatggt ataattggat gtgtattaga tcatccaatc
    33721 agaggtgttt attatgatta aagtgatttt taaaagaata actgaacttc cagagtttag
    33781 attatttgtt gattattggt atgtctggtt ttttattgtt gcattgctga taggtctaga
    33841 tgtcatatta gaaagaaaac ttaagtaaga gttgagttaa taaattgaat tcctaaatga
    33901 gatattttgt agcccatatt ttgtttgccg acatcaccta atactatgga gctaatcgtt
    33961 ttttctaaca gaccaaaatc agataatcta tttaagctac catctattag ctcttgtgaa
    34021 tagccagaca tgctgatatt tccagtgaaa ttttcgtttc cacgactcat taagcatgct
    34081 aacaattgaa tctcaatcga tgttaattga ttcagtacaa gaagataata ctcctcgtta
    34141 tcccatgaag cgttgttaat tcgaagtaaa caattcttat aaagattttt atacatatct
    34201 attttatttt gtgcctttga tttttctacc tcgtcattta tcccttcgaa tattccaatg
    34261 agttggtcct tgttctctaa gttgaaaaca tcttctggaa tttgatcgcg aatgttttct
    34321 aatgattcgt ttaaattttt gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta
    34381 gacccaaaat aaagtgactg cacaacgcca ccaatataag gaattgctcc aataccgcct
    34441 tcaactatta attcaagata ttcttttttg gttagttttt cattgctatt atccatagtt
    34501 acctcctatt ttgtcatttc ctttacaagc attgtgactc ctgatggcac tcttttgtat
    34561 aggttttatt attgtgcaca atctaactct tagtggggcg tggttcattt attttttact
    34621 tactttacta ttaatcatat cccattgagc ataggatttt tctatgttcg atcctcttga
    34681 actaatttct cctgtaaaag ttactttatc gccctttgta aaggtatcag cattgtttaa
    34741 gtccttcgca aaaattacat atgaaacttt aggtgttgca gaaatatcat tccaactttt
    34801 atcatcgtac tctttgtctg ctataatggc tattctagta gtcattgatt cgataacagt
    34861 tccttcaaaa gtatatgttt taccactaat atatttagta aaagctgtat tttgctcagt
    34921 agctggagta atagaataat aactatctaa gaaagcaccc cagtcatgat tatttgtctc
    34981 taaaaattct tcaaattgag ttaggctttt attgctgctg ttagatgtag tagtttgagt
    35041 atcagatgat gagctttcct gtttgttatt gtcatttgcc tcgtttgatg aggaaagcga
    35101 gaaacctacg gtagtaaaaa taatcccgat aataaggaga atcataggtg ttttcttatt
    35161 tgtttttttg ataaatctta caattaacat gattaaacct ataataaata aaacaatacc
    35221 agtaaaacca aatataccgt ccattaatag atcctctttc tcttttgata ttgaatatta
    35281 taaaccaagc aattcttttt tcttaaaatc gaattcttct tgattaataa tcccttcatc
    35341 taacaactct ttatactttt taatttcatc tgcacttgaa atttgtgttt ctttggaatt
    35401 attatctaat atagattgtt gcttctctat atattttttt aaattttcca tttcattcca
    35461 atattttttt gtaaacataa cggtgttttc atctttcgtg gccgctaata tacctccgcg
    35521 actttcgttt ccgcctagca aggtaaattg tatataacca ttactcatac ctggtttttt
    35581 taattgtacg gctgatatat ttttaaaggg gatagttttt tctcctttaa taccttgatt
    35641 aactaaattt ataaatcctt ttctaataat tgaaatagaa tcatcagtta ctttaatcaa
    35701 ggtttttcct ggagatttta ttaaaatctc tttttccatt ttttcttcct catttctgtg
    35761 atatgatttt ttttgtagaa gctcatatat gaggaaagag cccgtgttgc agcacgggct
    35821 tttttaatgt tttgctgaaa tcggtttttt taataattct tgatgtattc atacatattg
    35881 ccttgcgtaa gaatattttt tcttaaaata gcattggcag acagcataac aagtgtatct
    35941 gagcttattc tgaatagaat aatactccat aaatttttcg agattaaact gagattcatc
    36001 agtcagttcg ttctcaatat agatatttaa taaaattaga atagctattt tatctgcttc
    36061 tgtttcaaat tttgagtgaa aagttgtaga agtatcgtac aaaactgaaa attcaaaatg
    36121 agaggcgctg aaatgtgcaa gttcatgaga taaatgaaag gcttctgcag tttcaccgta
    36181 tagattttca ttcaaaaaaa tgattctggg ttttggatag tagaaaccag gctctttcat
    36241 ctccatataa actactttca aattgtattc gctcaacatt tctttcaatt tcaaatacat
    36301 acaaaccatc actccaacta ttcattttcc tctaaagctt tagcaattgc aatcgcttta
    36361 cgcattgtct ccttagatat ttcttttcca tcaaaagaaa aaacagtatc gtcttctgat
    36421 aaatccacat gtttaggggt ttctctttct tctctaccta gaaggtagtc tacagagaca
    36481 tcgaaatagt cagcaatccg acttaattca tctgaattag gagtattatt tttccacttt
    36541 gctagataac cgttagaata tccaaaatta atttctaact gccgtataga tatctttttc
    36601 ctcttcgcca agtcttttat tatttcatag gtattcatcg ataaaacaac ctttctaaat
    36661 gtttacaaaa aaagtttaga aaaatacgca gaaataattt gacta
//
LOCUS       DASJTC010000016        34960 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.016, whole genome shotgun sequence.
ACCESSION   DASJTC010000016 DASJTC010000000
VERSION     DASJTC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.016"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="U4B19_000752"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="U4B19_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEN2076511.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="U4B19_000753"
     CDS             complement(2020..2922)
                     /locus_tag="U4B19_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2076512.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="U4B19_000754"
     CDS             complement(2919..3611)
                     /locus_tag="U4B19_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEN2076513.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="U4B19_000755"
     CDS             complement(3654..4295)
                     /locus_tag="U4B19_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2076514.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="U4B19_000756"
     CDS             complement(4333..4647)
                     /locus_tag="U4B19_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076515.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="U4B19_000757"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="U4B19_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="U4B19_000758"
     CDS             complement(5984..6916)
                     /locus_tag="U4B19_000758"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEN2076516.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="U4B19_000759"
     CDS             complement(6913..7689)
                     /locus_tag="U4B19_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEN2076517.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="U4B19_000760"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="U4B19_000760"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEN2076518.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="U4B19_000761"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="U4B19_000761"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEN2076519.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="U4B19_000762"
     CDS             complement(12014..13294)
                     /locus_tag="U4B19_000762"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEN2076520.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="U4B19_000763"
     CDS             complement(13313..14239)
                     /locus_tag="U4B19_000763"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEN2076521.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="U4B19_000764"
     CDS             complement(14252..15535)
                     /locus_tag="U4B19_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEN2076522.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="U4B19_000765"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="U4B19_000765"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEN2076523.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="U4B19_000766"
     CDS             complement(16323..17234)
                     /locus_tag="U4B19_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2076524.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="U4B19_000767"
     CDS             complement(17231..17695)
                     /locus_tag="U4B19_000767"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEN2076525.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="U4B19_000768"
     CDS             complement(17700..18398)
                     /locus_tag="U4B19_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2076526.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="U4B19_000769"
     CDS             complement(18385..18771)
                     /locus_tag="U4B19_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076527.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="U4B19_000770"
     CDS             complement(18918..20588)
                     /locus_tag="U4B19_000770"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEN2076528.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="U4B19_000771"
     CDS             20771..21004
                     /locus_tag="U4B19_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEN2076529.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="U4B19_000772"
     CDS             complement(21103..21534)
                     /locus_tag="U4B19_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEN2076530.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="U4B19_000773"
     CDS             21613..22026
                     /locus_tag="U4B19_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEN2076531.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="U4B19_000774"
     CDS             complement(22064..23212)
                     /locus_tag="U4B19_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2076532.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="U4B19_000775"
     CDS             complement(23334..23930)
                     /locus_tag="U4B19_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2076533.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="U4B19_000776"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="U4B19_000776"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEN2076534.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="U4B19_000777"
     CDS             27160..27591
                     /locus_tag="U4B19_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2076535.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="U4B19_000778"
     CDS             complement(27636..28985)
                     /locus_tag="U4B19_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2076536.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="U4B19_000779"
     CDS             complement(29055..30218)
                     /locus_tag="U4B19_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEN2076537.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="U4B19_000780"
     CDS             30347..30670
                     /locus_tag="U4B19_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076538.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="U4B19_000781"
     CDS             complement(30853..32202)
                     /locus_tag="U4B19_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2076539.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="U4B19_000782"
     CDS             complement(32271..33224)
                     /locus_tag="U4B19_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEN2076540.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="U4B19_000783"
     CDS             complement(33326..34516)
                     /locus_tag="U4B19_000783"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEN2076541.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9951 a         7641 c         6120 g        11248 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa
//
LOCUS       DASJTC010000017        34663 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.017, whole genome shotgun sequence.
ACCESSION   DASJTC010000017 DASJTC010000000
VERSION     DASJTC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.017"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            591..1280
                     /locus_tag="U4B19_000784"
     CDS             591..1280
                     /locus_tag="U4B19_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2076542.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGIQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="U4B19_000785"
     CDS             1267..2475
                     /locus_tag="U4B19_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2076543.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="U4B19_000786"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="U4B19_000786"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEN2076544.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="U4B19_000787"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="U4B19_000787"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEN2076545.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="U4B19_000788"
     CDS             complement(6578..7273)
                     /locus_tag="U4B19_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEN2076546.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="U4B19_000789"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="U4B19_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEN2076547.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="U4B19_000790"
     CDS             complement(8472..8663)
                     /locus_tag="U4B19_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEN2076548.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="U4B19_000791"
     CDS             complement(8684..9574)
                     /locus_tag="U4B19_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEN2076549.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="U4B19_000792"
     CDS             complement(9561..10328)
                     /locus_tag="U4B19_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2076550.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="U4B19_000793"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="U4B19_000793"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEN2076551.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="U4B19_000794"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="U4B19_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEN2076552.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="U4B19_000795"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="U4B19_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEN2076553.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="U4B19_000796"
     CDS             14899..15282
                     /locus_tag="U4B19_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076554.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="U4B19_000797"
     CDS             complement(15450..16208)
                     /locus_tag="U4B19_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEN2076555.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="U4B19_000798"
     CDS             complement(16226..17044)
                     /locus_tag="U4B19_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEN2076556.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="U4B19_000799"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="U4B19_000799"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEN2076557.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="U4B19_000800"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="U4B19_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEN2076558.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="U4B19_000801"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="U4B19_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEN2076559.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="U4B19_000802"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="U4B19_000802"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEN2076560.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="U4B19_000803"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="U4B19_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEN2076561.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="U4B19_000804"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="U4B19_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEN2076562.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="U4B19_000805"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="U4B19_000805"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEN2076563.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="U4B19_000806"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="U4B19_000806"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEN2076564.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="U4B19_000807"
     CDS             27086..27385
                     /locus_tag="U4B19_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEN2076565.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="U4B19_000808"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="U4B19_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN2076566.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="U4B19_000809"
     CDS             27973..28209
                     /locus_tag="U4B19_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEN2076567.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="U4B19_000810"
     CDS             28352..30325
                     /locus_tag="U4B19_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEN2076568.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="U4B19_000811"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="U4B19_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEN2076569.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="U4B19_000812"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="U4B19_000812"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEN2076570.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="U4B19_000813"
     CDS             33204..34496
                     /locus_tag="U4B19_000813"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEN2076571.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10566 a         6735 c         6853 g        10509 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atacaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aac
//
LOCUS       DASJTC010000018        33677 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.018, whole genome shotgun sequence.
ACCESSION   DASJTC010000018 DASJTC010000000
VERSION     DASJTC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.018"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            214..498
                     /locus_tag="U4B19_000814"
     CDS             214..498
                     /locus_tag="U4B19_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEN2076572.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(815..1198)
                     /locus_tag="U4B19_000815"
     CDS             complement(815..1198)
                     /locus_tag="U4B19_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEN2076573.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(1200..1784)
                     /locus_tag="U4B19_000816"
     CDS             complement(1200..1784)
                     /locus_tag="U4B19_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEN2076574.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="U4B19_000817"
     CDS             complement(1831..3306)
                     /locus_tag="U4B19_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEN2076575.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="U4B19_000818"
     CDS             3487..3798
                     /locus_tag="U4B19_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076576.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="U4B19_000819"
     CDS             complement(3913..6249)
                     /locus_tag="U4B19_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2076577.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="U4B19_000820"
     CDS             6391..7059
                     /locus_tag="U4B19_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2076578.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="U4B19_000821"
     CDS             7062..8105
                     /locus_tag="U4B19_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2076579.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="U4B19_000822"
     CDS             complement(8342..9223)
                     /locus_tag="U4B19_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2076580.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="U4B19_000823"
     CDS             9382..10110
                     /locus_tag="U4B19_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEN2076581.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="U4B19_000824"
     CDS             10266..10583
                     /locus_tag="U4B19_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2076582.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="U4B19_000825"
     CDS             10576..11184
                     /locus_tag="U4B19_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEN2076583.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="U4B19_000826"
     CDS             11239..12030
                     /locus_tag="U4B19_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEN2076584.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="U4B19_000827"
     CDS             12168..12656
                     /locus_tag="U4B19_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEN2076585.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="U4B19_000828"
     CDS             12707..13579
                     /locus_tag="U4B19_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEN2076586.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="U4B19_000829"
     CDS             13755..14117
                     /locus_tag="U4B19_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2076587.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="U4B19_000830"
     CDS             14126..14878
                     /locus_tag="U4B19_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2076588.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="U4B19_000831"
     CDS             14892..16694
                     /locus_tag="U4B19_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2076589.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="U4B19_000832"
     CDS             16971..18011
                     /locus_tag="U4B19_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2076590.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="U4B19_000833"
     CDS             complement(18063..20207)
                     /locus_tag="U4B19_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEN2076591.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="U4B19_000834"
     CDS             complement(20564..21937)
                     /locus_tag="U4B19_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2076592.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="U4B19_000835"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="U4B19_000835"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEN2076593.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="U4B19_000836"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="U4B19_000836"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEN2076594.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="U4B19_000837"
     CDS             complement(23385..24398)
                     /locus_tag="U4B19_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2076595.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="U4B19_000838"
     CDS             complement(24479..25078)
                     /locus_tag="U4B19_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2076596.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="U4B19_000839"
     CDS             25264..25965
                     /locus_tag="U4B19_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2076597.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="U4B19_000840"
     CDS             25975..28686
                     /locus_tag="U4B19_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2076598.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="U4B19_000841"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="U4B19_000841"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEN2076599.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="U4B19_000842"
     CDS             complement(30825..32006)
                     /locus_tag="U4B19_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2076600.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..>33677
                     /locus_tag="U4B19_000843"
     CDS             32258..>33677
                     /locus_tag="U4B19_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEN2076601.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
BASE COUNT        10661 a         6038 c         6851 g        10127 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttattt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaccttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaa
//
LOCUS       DASJTC010000019        33264 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.019, whole genome shotgun sequence.
ACCESSION   DASJTC010000019 DASJTC010000000
VERSION     DASJTC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.019"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(42..230)
                     /locus_tag="U4B19_000844"
     CDS             complement(42..230)
                     /locus_tag="U4B19_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEN2076602.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            500..862
                     /locus_tag="U4B19_000845"
     CDS             500..862
                     /locus_tag="U4B19_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2076603.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            914..2596
                     /locus_tag="U4B19_000846"
     CDS             914..2596
                     /locus_tag="U4B19_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEN2076604.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2713..4749
                     /gene="recG"
                     /locus_tag="U4B19_000847"
     CDS             2713..4749
                     /gene="recG"
                     /locus_tag="U4B19_000847"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEN2076605.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4798..5799
                     /gene="plsX"
                     /locus_tag="U4B19_000848"
     CDS             4798..5799
                     /gene="plsX"
                     /locus_tag="U4B19_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEN2076606.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5921..6163
                     /gene="acpP"
                     /locus_tag="U4B19_000849"
     CDS             5921..6163
                     /gene="acpP"
                     /locus_tag="U4B19_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEN2076607.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6431..7435
                     /locus_tag="U4B19_000850"
     CDS             6431..7435
                     /locus_tag="U4B19_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2076608.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7436..8380
                     /locus_tag="U4B19_000851"
     CDS             7436..8380
                     /locus_tag="U4B19_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2076609.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8380..9342
                     /locus_tag="U4B19_000852"
     CDS             8380..9342
                     /locus_tag="U4B19_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2076610.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9369..10283
                     /locus_tag="U4B19_000853"
     CDS             9369..10283
                     /locus_tag="U4B19_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2076611.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10309..12090
                     /locus_tag="U4B19_000854"
     CDS             10309..12090
                     /locus_tag="U4B19_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2076612.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12438..13124
                     /locus_tag="U4B19_000855"
     CDS             12438..13124
                     /locus_tag="U4B19_000855"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEN2076613.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13144..16725
                     /gene="smc"
                     /locus_tag="U4B19_000856"
     CDS             13144..16725
                     /gene="smc"
                     /locus_tag="U4B19_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEN2076614.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16734..17543
                     /locus_tag="U4B19_000857"
     CDS             16734..17543
                     /locus_tag="U4B19_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2076615.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17554..18552
                     /gene="ftsY"
                     /locus_tag="U4B19_000858"
     CDS             17554..18552
                     /gene="ftsY"
                     /locus_tag="U4B19_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEN2076616.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18739..21006
                     /gene="gshAB"
                     /locus_tag="U4B19_000859"
     CDS             18739..21006
                     /gene="gshAB"
                     /locus_tag="U4B19_000859"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEN2076617.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21095..21547)
                     /locus_tag="U4B19_000860"
     CDS             complement(21095..21547)
                     /locus_tag="U4B19_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEN2076618.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21697..22647
                     /locus_tag="U4B19_000861"
     CDS             21697..22647
                     /locus_tag="U4B19_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN2076619.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22640..23599
                     /locus_tag="U4B19_000862"
     CDS             22640..23599
                     /locus_tag="U4B19_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEN2076620.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23596..24351
                     /locus_tag="U4B19_000863"
     CDS             23596..24351
                     /locus_tag="U4B19_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2076621.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24374..25327
                     /locus_tag="U4B19_000864"
     CDS             24374..25327
                     /locus_tag="U4B19_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2076622.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25971..26267)
                     /locus_tag="U4B19_000865"
     CDS             complement(25971..26267)
                     /locus_tag="U4B19_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEN2076623.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26622..26978
                     /locus_tag="U4B19_000866"
     CDS             26622..26978
                     /locus_tag="U4B19_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2076624.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26988..27887
                     /locus_tag="U4B19_000867"
     CDS             26988..27887
                     /locus_tag="U4B19_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEN2076625.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28404..29135
                     /locus_tag="U4B19_000868"
     CDS             28404..29135
                     /locus_tag="U4B19_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2076626.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29280..31307
                     /locus_tag="U4B19_000869"
     CDS             29280..31307
                     /locus_tag="U4B19_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN2076627.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31415..32512
                     /locus_tag="U4B19_000870"
     CDS             31415..32512
                     /locus_tag="U4B19_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEN2076628.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32819..>33264
                     /locus_tag="U4B19_000871"
     CDS             32819..>33264
                     /locus_tag="U4B19_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2076629.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT        11138 a         5762 c         7124 g         9240 t
ORIGIN      
        1 aaaaaaccgt tcactatctg aacggtccaa gtatcattca attaaacgcg ttcaactttt
       61 cctgatttca aagcacgagt tgacacccaa acttttttag gttttccgtc gaccataacg
      121 cgaacttttt gtaagttagg tttaacagta cgttttgttg agttcatagc gtgtgaacgg
      181 ttgtttccac tttttgtctt gcgacctgta aagtaacaaa cttttgccat ggtaatttcc
      241 tcctttgcct tgatttggag caatagaata attcagctca taccagacta acatatcata
      301 tagaatggaa atttgcaagt acattttcat agatacaatt gacaattgag aagaataatt
      361 gtaaattaaa caaggttata cctgaaattt tgcaaggttt ttgagtactt tgggtaactg
      421 ccttttattt caagttgcgc tatgataaaa tatgatagaa gaaaaatggg agttttttac
      481 ccgttaagga ggctcattca tggctgtaaa aattcaaacg tcagctggaa ccattgaaat
      541 taccaatgat gtaattgcaa cagttgttgg tggcgccgcg acagatgtgt tcggtgttgt
      601 aggaatggcc agcaaaaatc aaattaaaga taatattact gagattttgc gtaaagaaaa
      661 ttactcaaga ggagtagttg tccgccaaga agataatggt attgctgtag atgtttatac
      721 gattatcagc tatggtacga aaatctctga agtttcccgc aacgtccaag aaaaagtgaa
      781 atacaatcta gaggcattat taggcgtaac tgccaattca gttaatgtct ttgtacaagg
      841 tgttcgtgta ctgccagact aggcaggaga ctggctggta actcagcagt cgatctgaag
      901 gaggattatc taggtgagtg taacagaaat aagtgcaagc cagttccagg aaatggtcca
      961 ggcaggtgcg aatcgtctgc aagtaaatgc agaatacgtc aattcattaa atgtattccc
     1021 tgtgccagat ggcgatacgg gaacaaatat gaatctatct atgactagtg gagctaaggc
     1081 agtagtagat tcaacctcag aaaaagtagg agaattagca gcaatgcttt caaaaggatt
     1141 gctgatgggt gcgcgcggaa attccggcgt tattttgtct caattgtttc gtggattttc
     1201 taagcaaatt ccagatgttg tctcattaaa tgcttccgat cttgctgcag ccttcactca
     1261 tggagttgaa actgcgtata aagcagtaat gaaaccagta gaaggaacga ttttgacagt
     1321 agctagagaa gcagcgaaag ctggagaaaa gaaagcaaaa agtactgatg atgtcgttga
     1381 agtaatgaca gctgttgtaa aaggggcaaa gcgtgcttta gctaaaactc cagacttgct
     1441 tccagtccta aaagaagtcg gtgtcgtaga cagtggaggg caaggactgc tgtttgttta
     1501 tgagggcttt ttaagtgcat tgaatggaga attccaagcg gatgaaacat atgaaccttc
     1561 tccagcagaa atggatgata tggtcaatgc agaacatcat cgcagtgtcc aaggtcaaat
     1621 ggcgacagaa gatatccatt ttggttactg tacagaaatc atggtaaaaa tcggagaagg
     1681 acctacagtc gatagtacat tcgattacga agaattccgt aactatttag atggtatagg
     1741 agattcttta cttgtggtca atgatgacga gatcatcaaa gtccacgtgc atacagaaaa
     1801 cccaggagaa gtcttgaatt atggacaaaa attcggttca ttagtcaaaa tcaaagtcga
     1861 caacatgcgc ttgcagcatg agacgatttt ggaacacgat gaagaagtga acgagtttgc
     1921 gaaaaaagaa acaccacgta tcccgtatgg tatcgtagcg atcgctgctg gtaaaggcgt
     1981 ccaagagctg tttgaaagtt taggcgctaa ttatgttatc agtggtgggc aaacaatgaa
     2041 tccaagtacg gaagacattg ttaaagcgat tcaagaagta aacgcagata aagtgattgt
     2101 cttgccaaac aataagaaca tcttcatggc agctgatcaa gcagcagaag tagcagatat
     2161 cccagtagca gttgttcctt caagaacgat ttcccaagga ttgacagcta tgctttcatt
     2221 caatgatcaa gcttctttag aagaaaacaa agaaacgatg acagaaatgt tgtctagcgt
     2281 tgtgagcggt caagtcactc acgcgatcag agatacctca attgacggtg tagagatcaa
     2341 agaaggagac tttttgggca tgatcgatgg aaaaatcctg atctctaacc cagatatcac
     2401 agcaacagcg ttaagtacac tagaacaaat gatcaatgag gacacagaga tcgtaacgat
     2461 cttggcaggt gatgaaggaa cagttgaaca agcaaatgaa tttgctgaga aactaacaga
     2521 aaaacatcct gatttggaaa tcgagatcca ccaaggagat cagcctgtct atccttatct
     2581 attttctgct gaataatttc tgtgtcagaa tatctttcta acacagtatt cattcataaa
     2641 aggccttcgg ttagtgatca ggtatgatcg ctgaccaagt cctttttttg ttaagggagg
     2701 gaggaaaaat ttatgcgttc tttagatgat ccaatcacta tgctggcagg agtcggagaa
     2761 aaaagagcag ctagtcttgc cagcttgggc atccaaacga ttgaagactt actgacttat
     2821 tatcctttcc gctacgaaga tattcaggaa agaaatctaa acgagatcca agatcaagaa
     2881 aaggttactt tgaaaggaat cgtagtctcg cctcctgtga tgaatcgatt tggttataaa
     2941 aaaagccgat tgcaatttcg tatgatgcaa gaacatgatg tgttcaacgt ttcttttttt
     3001 aatcaacctt atctaaaaga aaaagtcatt ttatcggagg aaatcgccgt atacggtaaa
     3061 tgggatgcaa agcgtaaagc attgaacggg atgaagattt taggttctca aacaatggat
     3121 gacttttcgc cgatatatca cgtcaacaaa tcaattcgtc aaacgacatt agttgatcta
     3181 atcaaaaaag ggtttcaaga atacggcaca ttgattgaag aaaatctgcc aaacaacctt
     3241 gtggagaaat atcgactgct tgaccgagct tctgctgtaa gaagtatgca ttttccaaaa
     3301 gaccacgaag aaaaccatca agccaaacga cgtgtcgttt ttgaagagtt ctttttattt
     3361 caaatgcgtc ttcaaggatt gaaaaaagca gaaaaagcag aaacgaatgg tttggagatc
     3421 ctttatgatg tccaaaaatt gaaagaattt actcaaaagc taccttttga actgacaaat
     3481 gcacaaaaac gtgtaacgaa tgaaatctgt cgggatcttc gcagtccgca gcatatgcag
     3541 cgacttttgc agggagatgt aggtagcgga aaaacagtgg ttgccgcgat tgcgttatac
     3601 gctaccgtta cagcaggttt ccaaggtgcg ttgatggtgc ctacagaaat cttggcacaa
     3661 caacatatgg aaagcttgca acagctgttt gatcccaatg aagtaaccat cgcattgctt
     3721 acggggtcaa cgaaagcaaa agaacgtcgt gagctccttg aaaaactaga gcaaggggaa
     3781 attaacatcg tgatcggcac acatgcattg atccaagatg gagtcgaatt tcagaattta
     3841 ggtctagtta ttacggatga gcagcaccgt tttggcgtga accagcggaa agtacttcgt
     3901 gaaaaaggct ggcgaccaga tgtcttattt atgacagcga ctccgattcc tagaacatta
     3961 gctattacag catatggtga gatggatgta tctatcattg atgaacttcc agctggacgt
     4021 atcccgatcg aaacacgctg ggtaagaaca cctcaattgg acagtgtact ggattggacg
     4081 aaaaaagaac tggcaagagg acatcaaatg tatgtcatct gtccgctgat tgaaggatca
     4141 gaagcattag atgtaaaaaa tgcagtggaa atctatgaaa aacttcgtga actatttgca
     4201 ccaaaatatg aagttggctt gttacatggt aaaatgaaga atcaggaaaa agaagtaatc
     4261 atggaagcct tcaaagacaa ccagatgcag atattagttt cgactactgt aattgaagta
     4321 ggggtaaacg tgccgaacgc gaccgtcatg ctgatcatgg atgcggatcg atttggattg
     4381 gcacagctgc atcaacttcg tggacgtgtt ggacgcggaa gtgatgcttc ctactgtatc
     4441 ttagttgcta atcctaaaaa tgaactaggt gtggaacgga tgaaaatcat gacagagacc
     4501 accaatggtt ttgtactaag cgaaaaagat ctggaattac gtggtcctgg tgaagtattt
     4561 ggcttccgtc aatctggtct gcctcaattt gcgattgctg acttggtgac ggatggaaat
     4621 gttttggagg tagccagaga agaggctgca gccctttggc agataaagga ttggcagatg
     4681 cttcctgagt atcagccttt agcgcaaagc ctccaaatag aaaatcagga taatcgttat
     4741 ttcgattagc agactgtgct atactttata ctgatgacgg aaaaaggagt ggaaacaatg
     4801 aaaattgccg tagatgcaat gggcggagat cacgcacctc aagcaattgt agaaggcgtg
     4861 gctttagcgc agcaagattt tccagaagta gaattcttac tttacggaaa agaagcagaa
     4921 atcaagaaat acttaacaaa tgaaaaaaac atcacgatta tccatacaga cgaaaaaatc
     4981 aatagtgatg atgagccagt aaaagcaatt cgacgaaaaa agactgcatc aatggtcctt
     5041 gctgcacaag cagtgaaaaa cggtgaagca gatgctgtat tttcagcagg gaatacaggc
     5101 gcgctactag cagcagggtt gttcatcgta ggacgtatca aaaatatcga acgccctggc
     5161 ttgatgtcta ccttgccagt agtagggaaa gaaggtgccg gtttcgatat gctggatctt
     5221 ggcgctaacg cagaaaacaa accagaacat ttgttgcaat acggtatttt aggttctttt
     5281 tatgcacgta aagtccgagg tatcggacgt cctcgtgtcg cactcttaaa caacggaaca
     5341 gaagcaacaa aaggtagtga agtgacgaaa aaagcctatg aactccttca gaatgaaaca
     5401 agcttgaatt ttattggaaa cgtagaagct cgtgagctac tcaatggtgt ggctgatgta
     5461 gtggtcacag atggatttac aggcaacgct gttttgaaat ctattgaagg aacagcaatg
     5521 aatatgatga atctattaaa atctgctatt ctaaatgaag ggatcaaagg aaaaatgggc
     5581 gctatgcttt taaaagacgg cttacgttct ttgaaagcag aaatggatta ttccaaacat
     5641 ggcggtgccg ttttgtttgg cttgaaagca ccagtcatta aaactcatgg agcaacaggc
     5701 cccgatgctg ttcgctatac gatccgccaa atccacacaa tgcttgagac agatgtagtt
     5761 ggacaattag tcgaacaatt tgaaaaaagc gaagaatagt atttggatat ctgaagcaat
     5821 ccgttccatt gaaaaaaagg tggacattcc tgaaaaatgc cagtaaaatt agccatggtg
     5881 taggtaattg acaagtgtat gattcttgga ggtgaaaaag ttgactcgcg aagaagtatt
     5941 taacaaagta gcgaaaatta tttcaaatca ttttgagata gataccgata aagtgacgga
     6001 tgaattgaac atcaaagatg atttaaaagc tgattcaatt agtattatgg aattcgtttt
     6061 agagcttgaa gatgaattcg gtacggaaat ttcagatgaa gacgctgaac aaattgaaac
     6121 agtcggtggc gcagtagatt atatcagcag ccatcttaaa taatttcaaa taaaagcaaa
     6181 gaaggataaa aagctgcata cagcttttta tccttctttt tttaatcttt ttgacacgta
     6241 aaagtcattt ttctgataat tctggcgata aacagaaatt atgttgcaaa gtaaagcgat
     6301 tttctatata attattaata attcattaca caaagataag aataaccttt gttataaaac
     6361 gctttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
     6421 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
     6481 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
     6541 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
     6601 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
     6661 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
     6721 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
     6781 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
     6841 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
     6901 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
     6961 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
     7021 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
     7081 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
     7141 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
     7201 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
     7261 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
     7321 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
     7381 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
     7441 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
     7501 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
     7561 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
     7621 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
     7681 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
     7741 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
     7801 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
     7861 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
     7921 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
     7981 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
     8041 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
     8101 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
     8161 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
     8221 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
     8281 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
     8341 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
     8401 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
     8461 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
     8521 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
     8581 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
     8641 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
     8701 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
     8761 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
     8821 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
     8881 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
     8941 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
     9001 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
     9061 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
     9121 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
     9181 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
     9241 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
     9301 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
     9361 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
     9421 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
     9481 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
     9541 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
     9601 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
     9661 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
     9721 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
     9781 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
     9841 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
     9901 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
     9961 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
    10021 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
    10081 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
    10141 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
    10201 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
    10261 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
    10321 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
    10381 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
    10441 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
    10501 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
    10561 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
    10621 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
    10681 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
    10741 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
    10801 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
    10861 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
    10921 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
    10981 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
    11041 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
    11101 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
    11161 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
    11221 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
    11281 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
    11341 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
    11401 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
    11461 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
    11521 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
    11581 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
    11641 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
    11701 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
    11761 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
    11821 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
    11881 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
    11941 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
    12001 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
    12061 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
    12121 cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg
    12181 atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat
    12241 ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac
    12301 gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt accttttgac
    12361 cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca
    12421 taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca
    12481 tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg
    12541 agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt
    12601 tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat
    12661 tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat
    12721 gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc
    12781 gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg
    12841 gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg
    12901 ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg
    12961 atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt
    13021 ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat
    13081 tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa
    13141 gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc
    13201 attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat
    13261 attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga
    13321 aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa
    13381 gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt
    13441 gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc
    13501 agattaaaag atatccaaga attgttctta gattctggat taggaaaaga atcgttctca
    13561 atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg
    13621 atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag
    13681 aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa
    13741 gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa
    13801 gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa
    13861 gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct
    13921 gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc
    13981 gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag
    14041 acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa
    14101 gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa
    14161 gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat
    14221 ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca
    14281 gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg
    14341 aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag
    14401 caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa
    14461 aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa
    14521 caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta
    14581 atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac
    14641 tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt
    14701 attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa
    14761 gcagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa
    14821 gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca
    14881 atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt
    14941 atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac
    15001 ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg
    15061 atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc
    15121 atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa
    15181 ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa
    15241 aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt
    15301 acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc
    15361 ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa
    15421 ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca
    15481 gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc
    15541 gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat
    15601 cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc
    15661 caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca
    15721 gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta
    15781 tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga
    15841 aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa
    15901 gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta
    15961 gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa
    16021 gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa
    16081 cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt
    16141 gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg
    16201 tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa
    16261 gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa
    16321 ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct
    16381 cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc
    16441 atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag
    16501 gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc
    16561 caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat
    16621 gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg
    16681 gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca
    16741 aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg
    16801 agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag
    16861 gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg
    16921 actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg
    16981 aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg
    17041 atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga
    17101 agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg
    17161 atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag
    17221 atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa
    17281 gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat
    17341 tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg
    17401 atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg
    17461 tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga
    17521 tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt
    17581 gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt
    17641 ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga
    17701 acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt
    17761 tttgaaacag cgatacgaat caccgaagca ttacgtcaag aagtgaaact cagaaatgcg
    17821 aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg
    17881 gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg
    17941 tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc
    18001 aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc
    18061 gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga
    18121 ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat
    18181 gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta
    18241 gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt
    18301 gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca
    18361 acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta
    18421 cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc
    18481 gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta
    18541 aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt
    18601 tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta
    18661 agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat tagattcata
    18721 tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc
    18781 ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca
    18841 ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat
    18901 atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt
    18961 cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa
    19021 gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc
    19081 attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaaaaa
    19141 tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag
    19201 ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca
    19261 gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta
    19321 ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa
    19381 cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt
    19441 tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa
    19501 ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca
    19561 gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat
    19621 gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg
    19681 ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat
    19741 aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa
    19801 attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta
    19861 gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat
    19921 caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat
    19981 aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact
    20041 cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg
    20101 gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg
    20161 acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa
    20221 gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa
    20281 gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc
    20341 aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca
    20401 ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga
    20461 acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct
    20521 gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt
    20581 gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta
    20641 gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa
    20701 attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact
    20761 gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct
    20821 aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa
    20881 aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct
    20941 tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta
    21001 ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt
    21061 tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt
    21121 ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt
    21181 cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct
    21241 caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta
    21301 gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat
    21361 ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag
    21421 caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt
    21481 cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt
    21541 gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc
    21601 aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa
    21661 gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca
    21721 ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca
    21781 gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga
    21841 acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat
    21901 ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta
    21961 ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca
    22021 gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc
    22081 cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca
    22141 gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa
    22201 gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg
    22261 ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt
    22321 gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca
    22381 ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt
    22441 ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct
    22501 ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct
    22561 tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt
    22621 ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt
    22681 tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt
    22741 tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag
    22801 cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt
    22861 gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt
    22921 gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg
    22981 gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt
    23041 actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt
    23101 catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa
    23161 tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt
    23221 atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct
    23281 cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg
    23341 gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag
    23401 ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc
    23461 tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac
    23521 agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga
    23581 aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca
    23641 gtggttaaag atgtacagtt aacgattcct gaaaagaagt tgactgcttt tatcggtcca
    23701 aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca
    23761 ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa
    23821 aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta
    23881 gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc
    23941 gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact
    24001 ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat
    24061 tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg
    24121 cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat
    24181 atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt
    24241 acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg
    24301 gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat
    24361 taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt
    24421 agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca
    24481 aactgaaact acattgacga taacagatag taatggagac caaatcgagg tgccaaataa
    24541 tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt
    24601 tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa
    24661 aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca
    24721 gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat
    24781 tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa
    24841 tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga
    24901 attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact
    24961 agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt
    25021 catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg
    25081 tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga
    25141 cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt
    25201 agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta
    25261 tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt
    25321 taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac
    25381 attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt
    25441 attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca
    25501 ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat
    25561 tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga
    25621 taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat
    25681 ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc
    25741 agcgttctta ttactcaaaa caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca
    25801 tctgctgctt ctcggcaata agcccactag actagaaaat atgagaagct attttctgtc
    25861 cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acaatgttca
    25921 atcagctgac cttcggtatt agctcaaagc actgatttca caacctctat ttaggcatgt
    25981 gtctcagcaa gcggttgata gttgatgatc tgcatttttc gataaaaatc ggcgggaagc
    26041 ttactccacc gttttagtaa ttgatagaaa taactctctt ctgctttgat tttatactcg
    26101 cttaatatca atccaaaact attcgttttt tttgaagcta attttttata aatatccaat
    26161 gctttggttt tatcttcatt gacaaagagt gtttttactt caatataaga accctcgata
    26221 ttttgtgttt cttcgataaa atagatgttt tttatatatt ccttcatagt gattcctcca
    26281 aagaagtctt ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa
    26341 aaagtaattc tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt
    26401 atttaagaaa ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc
    26461 aagaaactga acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa
    26521 acgaatgact tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc
    26581 tgatgaataa agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa
    26641 aagaagcaca agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata
    26701 tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gatacggcat
    26761 ttcgattgat cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg
    26821 ctgatacacc agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc
    26881 catccacact tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct
    26941 tgagaaaagg caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg
    27001 cgatcatcag cgacctacat gtggacatca ataaattcgg cgaatcagag ctgatgcagc
    27061 tttggcaagt tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta
    27121 ataaagtaga ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga
    27181 catttcattt cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt
    27241 ttccagaccc acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt
    27301 tagggatgaa tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg
    27361 tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg
    27421 aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa
    27481 aacttgaggt gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat
    27541 ctgctcctta tgagcgttgg aacaaattaa atgcattttt aggatcagaa tcattcggag
    27601 aattacttga ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct
    27661 ttgaagataa atccatcggt cacacgattt acagctgcag accttttggt tattattatg
    27721 aatggcagct gacccgagat tttgtcttga aagaacagtt gctagacagc tatgatccta
    27781 tgaaattacg tgtcctcttg cgtgcccatc acccagcttt tcaggcctat caaacacaac
    27841 atttgcaaga ggaatttgaa caagcgatga caatcgttcc gtattgagac agcattagta
    27901 atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta
    27961 tcgtggatat gctgcttttt ttgcggaaaa taatcagcga atgtttattt gatcggatga
    28021 tttgtaactc gcagagtact agatgcaagc gtgtattctt tttttgaacg ataatcacaa
    28081 aaaaaaatat aatatacatt ttttatttaa ttataattaa tattgattga agtctaggca
    28141 gtcaagtcac attttgatag ataggagggg gacttacgaa ttattttaaa ggctatgaca
    28201 gctgtatttc tagattgtgt ttgcatcaag ttgcaataaa aactagaaat caaatcgggc
    28261 tgataatcat ttataactaa tcgatacata ggaccaaata taggtctatg ttcttttatt
    28321 tcataagtat cgtaaaacag aattttgcta tggattaatc caatcgactg atatgtttag
    28381 taaataaaga aagaggaatg tcaatgatga aaaaagaaat cgtattaaca gcagcaatga
    28441 tgttattcag catggtagca agtacgactt ttgtttctgc aactgaagta tacccaaagg
    28501 aatataacac agaaggaacg attacatttg aagcaggaga tgagggtgta actcctcctg
    28561 ttgatccaga gaatcctgat cccaataaac ctgtcgatcc atcagatcct ccaagccctg
    28621 gaacaggagg ggcattatcg attgattatg gatcaaaatt caaatttggg actcaaaaaa
    28681 tttctactgc ggataaaacg tattatgcag ctgctgatgt gatgaacgat gggtctagaa
    28741 aaccgacata tgtccaagtg acagatagga gaagtacctt atctgggtgg aaattaagtg
    28801 tctctcaacc agaacagttt aaaactgcca gtggggatga actagttgga gcacagctga
    28861 aatttacgaa agggcaggct gtttctttgg ttgatccaac atatacgccg caaacggtga
    28921 attctgaatt aacgttgaca ccgggaggaa acaatacgtt agcaattaat gctaaatcag
    28981 gaacaggtgt ggggacttgg gtctatcgat ttggtgcaaa tgaaaatgaa aatcaagatg
    29041 cggtacagct ttcggtaccc ggtaaatctg tcaagttagc gcaacaatat tccactaaat
    29101 tagtttggac attagaagat acacctaaca actagtttac tgaaaagaac tgttaaaaat
    29161 aaatacgtag tcatattgtc ttgcgaggta atcgagcaaa aattgtcaaa ggctaggcta
    29221 aatttgtttg gatacgaata gttcatctag gaatttaacg atcaggagga aaagatatat
    29281 tgaaaaagag gaggatcgtg ctcattttaa gcagtctctt agtgactaat atctttccga
    29341 tcatagggta ctctttggaa ttgaatcagt tggagcatgg aaatagaaga gacgaagaaa
    29401 gcaaactcac ttcgacattc gacagtttaa tagaatccaa ccagacacaa gaaagcagtg
    29461 aggatttaag tatttcgagt agtggagaga ccactgacaa cgtagaagct gagggttcgg
    29521 acaaacaagg actagaagat gcaccaattt ctgcggttga gctgcctgac gatcccaata
    29581 ttatttctat cgataaaatt tttcaaacac ctatcggtac tagcacgtcc atattggaaa
    29641 atgggaagtt actgcagttg aacccagcag aaaggtctca aagaggagct atttggtcaa
    29701 ataagaagat tagtttactc tcggatttta catttaaatc ttatttatac ttaggaaatc
    29761 aacgagatag agcgggagat ggcatgacct ttacattgac tggcgatcct agaatggcta
    29821 gtaatcccag tcaagttatt ggatcgccag gtatgggaat tggctcatat tctactcgtt
    29881 ctggccaacc ttacattgcg aatgcgctct cgattgagtt tgatacgtat aaaaatagtg
    29941 gttcttctga tcgaatggat cgagagataa ctgctgataa tagagggcat ggacatgtcg
    30001 cttttgtcac gcctaaacca aataataata attattctgg agagcatact ggcgtgacgg
    30061 tagctcctac atttctttct aatggcacat ggcgcttgtt aataattaaa tgggacgcgg
    30121 agcaaagaag tcttacttat gatttggaag gtgtcggtcg ctcttctcgg attatacctg
    30181 atctcattag gcagtttggt tctacagaag taaattgggg gtttacttct tcaacaggta
    30241 ctttttttca agaaaatgct gtcgctgtca cgcgacttcc atcgaatgtc acatcaacag
    30301 ctgatgtaaa agtcaacgat agtgattatg gggccatcgc tgaagctgga ggaaacgata
    30361 agataactat cagaaataca ttgaaggtcg agggggattt tcttgaatat aagaaaatgc
    30421 agactagtat cgacctccca caagaattaa ctattcccga gcatatcttg atcaatggaa
    30481 atcaaattga tgccaaagat atcttgataa ccggaaatcg agtcacgcta aatctgacag
    30541 attattttat tgctggagat acgttaataa tcgaattgga gacaactttg caggaagtaa
    30601 ttcctgaaaa agtactcact atgcattttg aataccttga aaacgggaat ttgattgagc
    30661 aatcgaatga aataaaccta aagatcgcaa agctgaagga gatagatatc catgtttatt
    30721 ataaagataa agacagtcag attgatattg ctgaaatgaa aacaatcact gggaaaatag
    30781 gagagaagta taaagaaact cctattgata ttgaagggta tgtttttgac tcggattcag
    30841 cgaatacaga aggcatcttc tccgaaaata cagaggatat ctatttttat tacagaaaag
    30901 gcaaacttta tctctcaact gctcctcaat atgtcaattt tggaaaacat aaaatatcca
    30961 gtagaccatt aagtgaattc ggacagtttt caggtgggtt gaaaattata gatgaacggg
    31021 cttttggaag ttggaaatta caattgaaac aagagaaagt tcttacaaat ggagattttg
    31081 agcttccaga agctctttcc tttgttacct ctgaaaatac aacggttata gggacatccg
    31141 ctgttacgat tttcgaaagt gatcaaaagg gtgaaagcga tttgtcctct cttttagatt
    31201 ctacagctca tcgtggaatc agaataaata ttcctgtaga aaaccaaaga atcggaacct
    31261 ttgaggggaa actatcttgg atacttgcag atgtcccagg aaattaacag gaagagaaga
    31321 atataacaaa attattgagt ttcaagaagg gcacaggtac cataggctct tgtgtcccac
    31381 agagacaaga ttttgttata gtcttctctt acatatgatt aaaggagcga ttttcttgag
    31441 accaaaacaa caattattta taacactcct agttatttta ggggcacttt ttaacaatat
    31501 atcagtgtca gctagtgcat ttaatttttc agtgacgccg ataacttcag agaatcaaat
    31561 cgacaagaga aaaacttact tcgatcttca acttgatccg gatcaggaag tagaagtaaa
    31621 agcggaattg agaaatgata cagaaaaaga agtgaaaatc gatatttctg tgaatagcgc
    31681 aaccaccaat tcaaatgtga tggttgaata cggaaaaaac gagattgaaa aagacatgag
    31741 cctgatcttt gatctggtag attatgtgag ctatccacag atagtgacgc taaaacctaa
    31801 aagtgtccaa accgttgctt ttcatgtcag gatgccgaat gagcgatttg atggtgtatt
    31861 ggctggtggg atcacattcc aagaaatcca gacggaaaag gaccaaacag aaactaagga
    31921 tcaaagctta tcgattgaga atgaatacac ctatattatt gctttgttga tgcaacagac
    31981 actgaacgaa gttgctccga accttactct tcatgaagtt aaaccagatc aaatcaatgc
    32041 aagaaatgtc atcttagcaa acgttcaaaa tgatcaaaaa acctatatca atcaagtagt
    32101 tattgaaaca aaaattacaa aaaaaggaca ttctgaagtt ttatatcaag aggaaaaaga
    32161 aggattgcag attgcgccga atacaaattt ttcttttcca actgctttaa atggtcaacc
    32221 gctaacccca ggtgaatatc accttacaat gactgtttta ggaaatgaaa atgaaaatgg
    32281 aaaattttcg agaaaaaaag gaaatactac gatcaactat accaatcaat gggtatttga
    32341 aaaagatttt acaatagacg gaaaagtagc caaggaattg aacacgacgg atgttacgat
    32401 taaaaaagac aataattgga tttatatctt agtagcttca cttttcttat tgtttatttt
    32461 attatgcata cttttgtttg ttttgagaaa aaaagaagag aaagaaaagt aaggttaaga
    32521 taacaaatag taaattttta ttattgtagg atggttgggc tctttttata acaatataaa
    32581 atataccatt ttattaataa aaatgcatac tagtaaatga tataattcta ataacaggtt
    32641 tgatcgcgag aaaggccaaa taaaacgctc tccaaaatgt taaaaaaaag acaggacgat
    32701 gtatattata caaacaagaa tcgctgacaa cagtgactcg gatccaggaa atcagtttta
    32761 tccccagcta gttggtgaat gcatggaaag taatatacgt agaaagagct gatgactaat
    32821 gaattatgag ttttttttaa gcaaagaaga tcacagaagt tattcattac ttaaatattt
    32881 agaagcaagc tcaaatttat cagagtcaat atcgaatgtt caagaagagt tgtcgctatc
    32941 gacatttctc ttgaaaaaaa cgatcgataa gctgaaacat gatttacaaa agttcgatct
    33001 agagaaaaat ttgcgagtaa ccgtcagtga attggatata tgtctggaaa ttaatggaaa
    33061 atgttctagc aaagctctgt tatctcgtta tataacggat tctttatcta tgaaaatggt
    33121 attggctttc tttcaggaaa aatatatctc agtagaaaat tttgctaaac agcatcacgt
    33181 gagttattct gtggcttata aagtattaca aagactaaaa agaaatctaa aaaaatatca
    33241 aatttttttt gaaaagagaa agtt
//
LOCUS       DASJTC010000020        32762 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.020, whole genome shotgun sequence.
ACCESSION   DASJTC010000020 DASJTC010000000
VERSION     DASJTC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.020"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(29..1261)
                     /locus_tag="U4B19_000872"
     CDS             complement(29..1261)
                     /locus_tag="U4B19_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEN2076630.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="U4B19_000873"
     CDS             1516..2085
                     /locus_tag="U4B19_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2076631.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="U4B19_000874"
     CDS             complement(2263..2703)
                     /locus_tag="U4B19_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEN2076632.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="U4B19_000875"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="U4B19_000875"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN2076633.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="U4B19_000876"
     CDS             3657..4580
                     /locus_tag="U4B19_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEN2076634.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="U4B19_000877"
     CDS             complement(4805..5044)
                     /locus_tag="U4B19_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2076635.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="U4B19_000878"
     CDS             complement(5049..5468)
                     /locus_tag="U4B19_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2076636.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /locus_tag="U4B19_000879"
     CDS             complement(5695..6933)
                     /locus_tag="U4B19_000879"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2076637.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="U4B19_000880"
     CDS             complement(7040..7675)
                     /locus_tag="U4B19_000880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2076638.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(8017..8652)
                     /locus_tag="U4B19_000881"
     CDS             complement(8017..8652)
                     /locus_tag="U4B19_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2076639.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="U4B19_000882"
     CDS             9164..10306
                     /locus_tag="U4B19_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEN2076640.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="U4B19_000883"
     CDS             10299..10505
                     /locus_tag="U4B19_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEN2076641.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="U4B19_000884"
     CDS             10584..11777
                     /locus_tag="U4B19_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2076642.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11982..13121
                     /locus_tag="U4B19_000885"
     CDS             11982..13121
                     /locus_tag="U4B19_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEN2076643.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13114..13914
                     /locus_tag="U4B19_000886"
     CDS             13114..13914
                     /locus_tag="U4B19_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2076644.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13914..14741
                     /locus_tag="U4B19_000887"
     CDS             13914..14741
                     /locus_tag="U4B19_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2076645.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14719..15453
                     /locus_tag="U4B19_000888"
     CDS             14719..15453
                     /locus_tag="U4B19_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2076646.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            15553..16419
                     /gene="rfbA"
                     /locus_tag="U4B19_000889"
     CDS             15553..16419
                     /gene="rfbA"
                     /locus_tag="U4B19_000889"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEN2076647.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16433..17005
                     /gene="rfbC"
                     /locus_tag="U4B19_000890"
     CDS             16433..17005
                     /gene="rfbC"
                     /locus_tag="U4B19_000890"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEN2076648.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17027..18055
                     /gene="rfbB"
                     /locus_tag="U4B19_000891"
     CDS             17027..18055
                     /gene="rfbB"
                     /locus_tag="U4B19_000891"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEN2076649.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18153..19004
                     /gene="rfbD"
                     /locus_tag="U4B19_000892"
     CDS             18153..19004
                     /gene="rfbD"
                     /locus_tag="U4B19_000892"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEN2076650.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19038..21071
                     /locus_tag="U4B19_000893"
     CDS             19038..21071
                     /locus_tag="U4B19_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076651.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21115..22395
                     /locus_tag="U4B19_000894"
     CDS             21115..22395
                     /locus_tag="U4B19_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEN2076652.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22605..23411
                     /locus_tag="U4B19_000895"
     CDS             22605..23411
                     /locus_tag="U4B19_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2076653.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23423..24643
                     /locus_tag="U4B19_000896"
     CDS             23423..24643
                     /locus_tag="U4B19_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2076654.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24633..26219
                     /locus_tag="U4B19_000897"
     CDS             24633..26219
                     /locus_tag="U4B19_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2076655.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26258..28396
                     /locus_tag="U4B19_000898"
     CDS             26258..28396
                     /locus_tag="U4B19_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2076656.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28764..29747)
                     /locus_tag="U4B19_000899"
     CDS             complement(28764..29747)
                     /locus_tag="U4B19_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEN2076657.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30125..31516
                     /locus_tag="U4B19_000900"
     CDS             30125..31516
                     /locus_tag="U4B19_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN2076658.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31532..32572
                     /locus_tag="U4B19_000901"
     CDS             31532..32572
                     /locus_tag="U4B19_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2076659.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32591..>32762
                     /locus_tag="U4B19_000902"
     CDS             32591..>32762
                     /locus_tag="U4B19_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN2076660.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGG"
BASE COUNT        10538 a         5692 c         6478 g        10054 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tagtcaaaaa gacatatata
    11941 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
    12001 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
    12061 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
    12121 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
    12181 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
    12241 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
    12301 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
    12361 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
    12421 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
    12481 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
    12541 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
    12601 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
    12661 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
    12721 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
    12781 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
    12841 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
    12901 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
    12961 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
    13021 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
    13081 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
    13141 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
    13201 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
    13261 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
    13321 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
    13381 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
    13441 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
    13501 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
    13561 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
    13621 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
    13681 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
    13741 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
    13801 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
    13861 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
    13921 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
    13981 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
    14041 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
    14101 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
    14161 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
    14221 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
    14281 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
    14341 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
    14401 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
    14461 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
    14521 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
    14581 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
    14641 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
    14701 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
    14761 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
    14821 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
    14881 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
    14941 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
    15001 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
    15061 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
    15121 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
    15181 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
    15241 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
    15301 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
    15361 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
    15421 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
    15481 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
    15541 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
    15601 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
    15661 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
    15721 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
    15781 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
    15841 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
    15901 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
    15961 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
    16021 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
    16081 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
    16141 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
    16201 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
    16261 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
    16321 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
    16381 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
    16441 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
    16501 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
    16561 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
    16621 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
    16681 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
    16741 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
    16801 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
    16861 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
    16921 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
    16981 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
    17041 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
    17101 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
    17161 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
    17221 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    17281 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    17341 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    17401 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    17461 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    17521 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    17581 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    17641 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    17701 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    17761 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    17821 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    17881 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    17941 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    18001 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    18061 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    18121 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    18181 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    18241 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    18301 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    18361 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    18421 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    18481 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    18541 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    18601 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    18661 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    18721 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    18781 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    18841 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    18901 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    18961 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    19021 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    19081 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    19141 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    19201 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    19261 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    19321 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    19381 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    19441 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    19501 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    19561 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    19621 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    19681 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    19741 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    19801 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    19861 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    19921 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    19981 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    20041 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    20101 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    20161 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    20221 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    20281 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    20341 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    20401 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    20461 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    20521 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    20581 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    20641 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    20701 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    20761 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    20821 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    20881 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    20941 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    21001 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    21061 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    21121 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    21181 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    21241 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    21301 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    21361 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    21421 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    21481 atttatctga ttgtcttttc ggtcttcctc ggaatcacgt tatatcgtgt atttacagag
    21541 atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg
    21601 atcaatacga atataatcgg aagttctttg atgactcttg ctttattgat cagtggattt
    21661 gccagttctt ttgaacgatg gtatgtacga atcatcggtg cagtagcagt tgttgcagct
    21721 tttctcggga tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg
    21781 atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcttac
    21841 acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc
    21901 aatttgttta ccggtcgaga agatatttgg cataaattct atcagacgct aggagaaaaa
    21961 tctgaacaaa ttttactagg aatgaaaaca tttatcttcc aacgaggaaa ccaattttta
    22021 ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg atttgggatt
    22081 gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac
    22141 gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat
    22201 acattgactt catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat
    22261 cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat
    22321 atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc
    22381 caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt
    22441 tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg
    22501 tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat
    22561 ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt
    22621 atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg
    22681 acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc
    22741 tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg
    22801 atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct
    22861 cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag
    22921 tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccact
    22981 tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa
    23041 tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt
    23101 ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc
    23161 tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc
    23221 ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat
    23281 ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca
    23341 cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag
    23401 atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa
    23461 atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa
    23521 gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg
    23581 tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat
    23641 tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt
    23701 gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata tctatttaaa
    23761 tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga
    23821 atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt
    23881 cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga
    23941 agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat
    24001 caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt
    24061 ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga
    24121 agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa
    24181 taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt
    24241 gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa
    24301 agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg
    24361 aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc
    24421 ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg
    24481 tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa
    24541 gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt
    24601 agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag
    24661 atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag
    24721 agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag
    24781 tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg
    24841 gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg
    24901 attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga
    24961 aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc
    25021 ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc
    25081 tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag
    25141 cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa
    25201 aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg
    25261 gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg
    25321 cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag
    25381 atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt
    25441 tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta
    25501 tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag
    25561 tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa
    25621 cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga
    25681 caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc
    25741 tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa
    25801 gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg
    25861 aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag
    25921 aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa
    25981 aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag
    26041 ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag
    26101 aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac
    26161 gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa
    26221 taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt
    26281 atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc
    26341 aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc
    26401 tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac
    26461 ccaaagcggt tttaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt
    26521 ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga
    26581 tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta
    26641 tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg
    26701 atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag
    26761 gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa
    26821 cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca
    26881 ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga
    26941 atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat
    27001 cagtctggca actaattctg cattggaaat agcagaagga gaatttatag cactcttaga
    27061 taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca
    27121 tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt
    27181 tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc
    27241 tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta
    27301 tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca
    27361 tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag
    27421 taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct
    27481 gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat
    27541 tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta
    27601 tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga
    27661 aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata
    27721 caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc
    27781 tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa
    27841 cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga
    27901 tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg
    27961 agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca
    28021 ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg
    28081 tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt
    28141 agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta
    28201 tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc
    28261 agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata
    28321 tattgcgcat gatccatttt acaatgataa tttaacaaaa gaaggaatcg gcgacttttc
    28381 gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac
    28441 ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg
    28501 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    28561 caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt
    28621 cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct
    28681 cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa
    28741 tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca
    28801 aaagccactc tatcaattat catgggttta aaataagaag tgatggagat aaccagattg
    28861 ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc
    28921 tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca
    28981 ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta
    29041 tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac
    29101 aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga
    29161 gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg
    29221 tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta
    29281 cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg
    29341 ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt
    29401 gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag
    29461 tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa
    29521 taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc
    29581 agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa
    29641 ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata
    29701 tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc
    29761 actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg
    29821 taatacaggt attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata
    29881 taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta
    29941 ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt
    30001 agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac
    30061 taggttttta aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt
    30121 atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga
    30181 tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat
    30241 cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat
    30301 tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt
    30361 gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc
    30421 gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct
    30481 tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa
    30541 aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc
    30601 aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat
    30661 caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga
    30721 aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac
    30781 atttgaaaac ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat
    30841 tgaagcatct ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat
    30901 catcgtttca cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt
    30961 agtcaaagtg acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca
    31021 aaaggaattt tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg
    31081 accagtttta gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc
    31141 tgtccgcttt gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt
    31201 aggaccacgt cctgaacgtc ctttcttcgt cgatcagttc aatgcagaaa acccttatta
    31261 ctacttacgg cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata
    31321 tgtctctgat tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt
    31381 gatgcttgat ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc
    31441 tcaaggactt gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga
    31501 aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt
    31561 accagtatac aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga
    31621 ttatgaagag tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat
    31681 cattcaagaa ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg
    31741 gcccggatta gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt
    31801 cgatgcagat gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa
    31861 gagtccaaaa gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag
    31921 aatcgtttcc gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt
    31981 tataaaagac atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg
    32041 ctatcgtagc gatattctga aaaataacca aattctattt aaaacttata atagttgtga
    32101 ggatcgaata tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa
    32161 agtagaatat atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa
    32221 caagttcgaa acatttaaag aattctattc attagcaaat actgtaacag acgatattaa
    32281 taaagacggt atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta
    32341 tagtacctct gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga
    32401 tccagtgatc cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac
    32461 aaagttgctt tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt
    32521 cgttgaagtg aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga
    32581 ggaaaatata atgaaaagaa ttttgcattt tcagggacga atgggtttag gtggagcaga
    32641 gtctttcatg atgaatttat atcgtaaaat tgatcgtaca aactatcaat ttgatttttt
    32701 gatttatgaa gattatgcag atgttcaaga ctatcattca gagattgaaa gattaggagg
    32761 cc
//
LOCUS       DASJTC010000021        32066 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.021, whole genome shotgun sequence.
ACCESSION   DASJTC010000021 DASJTC010000000
VERSION     DASJTC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.021"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            33..656
                     /locus_tag="U4B19_000903"
     CDS             33..656
                     /locus_tag="U4B19_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076661.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1106..2155
                     /locus_tag="U4B19_000904"
     CDS             1106..2155
                     /locus_tag="U4B19_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2076662.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2152..3033
                     /locus_tag="U4B19_000905"
     CDS             2152..3033
                     /locus_tag="U4B19_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2076663.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3036..3806
                     /locus_tag="U4B19_000906"
     CDS             3036..3806
                     /locus_tag="U4B19_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2076664.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4085..4939
                     /gene="aroE"
                     /locus_tag="U4B19_000907"
     CDS             4085..4939
                     /gene="aroE"
                     /locus_tag="U4B19_000907"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEN2076665.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5117..6280
                     /locus_tag="U4B19_000908"
     CDS             5117..6280
                     /locus_tag="U4B19_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2076666.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6397..6807
                     /locus_tag="U4B19_000909"
     CDS             6397..6807
                     /locus_tag="U4B19_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2076667.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6981..9209
                     /gene="topB"
                     /locus_tag="U4B19_000910"
     CDS             6981..9209
                     /gene="topB"
                     /locus_tag="U4B19_000910"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2076668.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9380..9664
                     /locus_tag="U4B19_000911"
     CDS             9380..9664
                     /locus_tag="U4B19_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076669.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9824..10060
                     /locus_tag="U4B19_000912"
     CDS             9824..10060
                     /locus_tag="U4B19_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2076670.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10057..10455
                     /locus_tag="U4B19_000913"
     CDS             10057..10455
                     /locus_tag="U4B19_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN2076671.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11122..12066)
                     /locus_tag="U4B19_000914"
     CDS             complement(11122..12066)
                     /locus_tag="U4B19_000914"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN2076672.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12362..13306)
                     /locus_tag="U4B19_000915"
     CDS             complement(12362..13306)
                     /locus_tag="U4B19_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2076673.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13527..14741
                     /locus_tag="U4B19_000916"
     CDS             13527..14741
                     /locus_tag="U4B19_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2076674.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14763..16778
                     /locus_tag="U4B19_000917"
     CDS             14763..16778
                     /locus_tag="U4B19_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEN2076675.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16861..18234)
                     /locus_tag="U4B19_000918"
     CDS             complement(16861..18234)
                     /locus_tag="U4B19_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN2076676.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18260..18544)
                     /locus_tag="U4B19_000919"
     CDS             complement(18260..18544)
                     /locus_tag="U4B19_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2076677.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18546..20561)
                     /locus_tag="U4B19_000920"
     CDS             complement(18546..20561)
                     /locus_tag="U4B19_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2076678.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20740..21045)
                     /locus_tag="U4B19_000921"
     CDS             complement(20740..21045)
                     /locus_tag="U4B19_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEN2076679.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21134..22165)
                     /locus_tag="U4B19_000922"
     CDS             complement(21134..22165)
                     /locus_tag="U4B19_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2076680.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22372..23247
                     /locus_tag="U4B19_000923"
     CDS             22372..23247
                     /locus_tag="U4B19_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2076681.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23399..24241)
                     /locus_tag="U4B19_000924"
     CDS             complement(23399..24241)
                     /locus_tag="U4B19_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2076682.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24333..26210)
                     /locus_tag="U4B19_000925"
     CDS             complement(24333..26210)
                     /locus_tag="U4B19_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2076683.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26207..27628)
                     /locus_tag="U4B19_000926"
     CDS             complement(26207..27628)
                     /locus_tag="U4B19_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2076684.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27631..31020)
                     /locus_tag="U4B19_000927"
     CDS             complement(27631..31020)
                     /locus_tag="U4B19_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN2076685.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31277..>32066
                     /locus_tag="U4B19_000928"
     CDS             31277..>32066
                     /locus_tag="U4B19_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2076686.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEK"
BASE COUNT         9891 a         5795 c         6172 g        10208 t
ORIGIN      
        1 aataaaaaat aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat
       61 cgagagtaca agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt
      121 attttcgacg tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt
      181 tattgccgga aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa
      241 ccagcaaatt gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg
      301 cgatatcggt gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt
      361 ttaatgatcc tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt
      421 atgatgtaca tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt
      481 tctttgatgg aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt
      541 ttgataagga gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag
      601 aagaagaatt ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca
      661 ttggcgcgat gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg
      721 tttgatcttc aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt
      781 cgattagact taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt
      841 atttcagcag aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg
      901 agactttccc atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga
      961 catgaacatt gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga
     1021 ccgctttggc cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa
     1081 agattgactt tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat
     1141 tattttagtt tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag
     1201 tacttcgacg aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca
     1261 tccggcatta gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa
     1321 agaaggagaa aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc
     1381 cacaatgagt caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc
     1441 tgccgctcaa tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac
     1501 ggatcctgtg ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg
     1561 tgtttctgat cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa
     1621 tgcaaaaaaa gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc
     1681 acaagcagaa gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc
     1741 cactaatgag attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt
     1801 tccattagat aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc
     1861 aaatatcccc gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat
     1921 cgggatcaat caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag
     1981 tggaaaagaa aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat
     2041 caatcaatct caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc
     2101 caaaatcatt acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta
     2161 tcagctattt cacaaggcat gctttgggca atattaggat tagggatttt catgacttat
     2221 cggatcttag actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc
     2281 tgtgtgacag ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt
     2341 gctggcatga tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt
     2401 attttagcag ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga
     2461 acaccaaatc tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg
     2521 ttacctgatt actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct
     2581 gtactgctat tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac
     2641 gaaacgatgg ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact
     2701 ttgtcgaacg gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca
     2761 gatgtaaata aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa
     2821 gtgatcttcc aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc
     2881 atttatcagc tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag
     2941 ttgttctccg ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg
     3001 aacttaaaaa cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa
     3061 atggcagaaa agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta
     3121 accttacaat cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa
     3181 gcacactgtt caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta
     3241 aagatcagct gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt
     3301 tccaagaccc caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg
     3361 cacaaaaaag aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag
     3421 attttttcag actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag
     3481 cgggtgaatt atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag
     3541 ctcctgagtt actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag
     3601 cattaatgcg attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca
     3661 cgcatcgaat ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga
     3721 aaatcgttca agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc
     3781 agttctttga agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact
     3841 tgaaaactca ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa
     3901 catatttggg tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt
     3961 ctttgctctg acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc
     4021 gtactttttc gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa
     4081 taagatgatt tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag
     4141 tttatcccca ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt
     4201 agctttccaa gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga
     4261 tatgttaggt gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga
     4321 ggtaagccag gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg
     4381 tttgattggc tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat
     4441 ttcgatcaaa aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac
     4501 tgttcaagct gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac
     4561 attcgctcaa gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa
     4621 agttcgtgac tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct
     4681 agtcaatgca acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat
     4741 aagcatgatg catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg
     4801 tttattaaaa gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat
     4861 ctaccaagga gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat
     4921 tgaaaagctg tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg
     4981 gtctcaagaa aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat
     5041 tatcaaaaaa tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa
     5101 acgaggagga accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa
     5161 acctctgaag cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat
     5221 ggaaaatgag gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt
     5281 gaaccaatac gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa
     5341 taaaaaattt ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc
     5401 gtggaaaaaa atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt
     5461 ttttattggt aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac
     5521 ctttcatggg caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac
     5581 acgcggagaa gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc
     5641 ccataagccg aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg
     5701 acaaaataag atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt
     5761 agtagaaaat tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga
     5821 aaaagtgatc gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa
     5881 tctggatggt gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca
     5941 gtacgcacgt ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca
     6001 agtaatgaac gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta
     6061 tgcagctggt aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa
     6121 aaacctcttt tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga
     6181 cggttcgtgg aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca
     6241 aatgaatcaa tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag
     6301 aagctgcgca aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc
     6361 ttatggtaga tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa
     6421 aatgagtcgc gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt
     6481 atcaccacat acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa
     6541 aatctaatca cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg
     6601 ccagaaggaa ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca
     6661 agaaatgaag aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag
     6721 atgccactgg atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc
     6781 atcaactggc agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa
     6841 gcagcaaacg gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt
     6901 atcttttttt tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat
     6961 taagtaacgg aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa
     7021 aagcctatgc cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg
     7081 atccattgtt tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg
     7141 ttgctccagg agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt
     7201 tcccagaaac cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa
     7261 aacgagaatt gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg
     7321 aagccatcgc gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt
     7381 tacgtttatg gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc
     7441 aacctggaaa gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt
     7501 ggctgattgg aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg
     7561 atggcagttt ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc
     7621 aagaagaaat caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga
     7681 caaacaaagg gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag
     7741 agttaacgaa ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc
     7801 ttgaagttac gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt
     7861 tgcagacaaa gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc
     7921 aaggattgta cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag
     7981 aaaatgagca tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag
     8041 aagaagtaca gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc
     8101 aagaacatca tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc
     8161 tttccaaatt acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg
     8221 ctgcagatta tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa
     8281 aaagcatcgg aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa
     8341 aaaagaaaga agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac
     8401 tgaaaagtat ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca
     8461 cagcaatgaa aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag
     8521 aagtagaagg aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga
     8581 agcattacat cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt
     8641 gtaaggcagt agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag
     8701 ggtacttgag aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca
     8761 aaaaattcat cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt
     8821 cagatgagat tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag
     8881 gaagatgtcc aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct
     8941 tgaatccgga atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa
     9001 cgaatctcag agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg
     9061 gtaaaaaagg gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt
     9121 cttttccatg gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa
     9181 gaaaaaataa tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt
     9241 ccgaaaagtg agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa
     9301 cgttggggta ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca
     9361 ttcattttta aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct
     9421 aaagtatcct gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc
     9481 accaacgttg attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca
     9541 taaaacgaca agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc
     9601 ggctatgatc attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg
     9661 ttaagaatac gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt
     9721 tcgtaagaaa aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa
     9781 tgtacttact atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa
     9841 gtataaaatt agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga
     9901 attcatgggt gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt
     9961 acgaatgatc aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa
    10021 tcaatatgaa gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa
    10081 attatcaaaa tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta
    10141 aaagatgcaa atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa
    10201 gaactctatc ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa
    10261 catccttttc ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa
    10321 ttgaataatt gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt
    10381 accactagtt cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa
    10441 accgcaataa aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa
    10501 ggaatatgct gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga
    10561 aaatatggat taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg
    10621 aaatagtggt ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata
    10681 ttatacaaag tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc
    10741 gtcatttttc catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt
    10801 actttttttg tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata
    10861 tgaacgaatt agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca
    10921 cagataaaca ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca
    10981 tatgtacgga tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta
    11041 ttagatatca ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt
    11101 ttagttcata ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt
    11161 tgatttgctg aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac
    11221 accattttcg tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc
    11281 agttaaataa gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat
    11341 acgtgcagta ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata
    11401 agcagcatta cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc
    11461 tttttctaca atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac
    11521 ttctgaatca ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa
    11581 tttcaatgcg atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac
    11641 acgtgatgtt ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt
    11701 tgaagcaaca acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg
    11761 catgattgac gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat
    11821 accagctgtg ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac
    11881 agccacattt tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc
    11941 tttttcttga ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc
    12001 ataagcgata cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt
    12061 tttcatgaat aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct
    12121 tgtttattca ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa
    12181 acgaattcct gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat
    12241 aatacaacca aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa
    12301 taagacagca atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt
    12361 cttaatgttt atttctccat tcattgggag tacatgttga atacttcttg aataatttga
    12421 ataaagaagt cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg
    12481 tttcttgaat cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg
    12541 aagcatcaaa atacattttc aatttattac taagatagac cggattgtag ccaaaatgag
    12601 ctgctgtttc ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa
    12661 gcatctcatt ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag
    12721 tcaattcgat cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg
    12781 tatcaaaata aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc
    12841 cgcgaaaatg aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag
    12901 aagaaagtag attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt
    12961 cttttaataa gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag
    13021 cttctttatc aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa
    13081 tttttaaaca tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg
    13141 gaacttccgc atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc
    13201 ctcggtggat ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca
    13261 ttccttgttc acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat
    13321 tcagctagtt ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca
    13381 cgctgtactg ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg
    13441 gacatgaact tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga
    13501 taagcgctta caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc
    13561 tgctgtcagt ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta
    13621 tgtcagctac ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct
    13681 cttttatggt cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa
    13741 aacaaactgt gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct
    13801 cacaaagtta cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga
    13861 tcaattcttt ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta
    13921 tgattggttt tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag
    13981 gacagctagt acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta
    14041 ttcttgtctt gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg
    14101 gcacagcccc aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt
    14161 ttctcttgct ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga
    14221 cgacttattc tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg
    14281 caagtatggc tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc
    14341 ttacactttc tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc
    14401 tgtatgtgat tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt
    14461 acttattcaa ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa
    14521 tcttattggt tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct
    14581 cttttgcttc tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt
    14641 tgattagcgc gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa
    14701 gagtagatga agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta
    14761 aaatgacaaa tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc
    14821 gccatggact gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact
    14881 ggtacatgga tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac
    14941 cttccatgtt taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac
    15001 atccatgtgg aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag
    15061 gacatacatc agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg
    15121 gctggacagc aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg
    15181 atatcgcaaa agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg
    15241 aaacggagat tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag
    15301 aactaatatc tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg
    15361 tagaagctat ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga
    15421 aagctcgtaa acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc
    15481 aacttcaagt ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt
    15541 cttctttact gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt
    15601 tgttttttat cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat
    15661 cattcatgat caatcaatat cttttttttg acgaggaaat caataaacga acgatattag
    15721 cattaagagg acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct
    15781 tgtggttgat tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca
    15841 tcgtctaccc taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag
    15901 ggtttattta tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag
    15961 aaggaacgct tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt
    16021 gcggagatat cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc
    16081 aagaaaaata tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg
    16141 gcgcttttat cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt
    16201 tcgctgtttt attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct
    16261 tattgaatga cactatcaca cagattacta ctccatattt caaattctat gaacaagatg
    16321 ctttatgcaa actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag
    16381 ggatgttatc tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa
    16441 atgaaaagta cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag
    16501 caagcccaat ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt
    16561 atgaagaatt tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac
    16621 ctatcaaaga tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg
    16681 atagaacagg cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc
    16741 cacttgtctt aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat
    16801 atcagaagac tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc
    16861 ttaattctgt tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa
    16921 tacgacaagt aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc
    16981 tagtttccca agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc
    17041 gattttaaga tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct
    17101 attgaaagca gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc
    17161 agaagcagct ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa
    17221 gcctatcata gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa
    17281 tacagcatta ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg
    17341 tacgagtttt ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac
    17401 cccttgaagg acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat
    17461 tacagcgttg tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc
    17521 aagtgcgaca tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa
    17581 gctgaaactt ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt
    17641 tccgatccat ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt
    17701 gtttccagtg atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt
    17761 tccttcgaag accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac
    17821 taaaatgact gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa
    17881 tttatatctt gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa
    17941 agcagtaggt gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac
    18001 tactccttga gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact
    18061 gattaaacca gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg
    18121 gatcacttct gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac
    18181 gaggatggag ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag
    18241 ttcctccctt gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt
    18301 cttcgctgtc aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg
    18361 tatctttcaa agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa
    18421 catccgtatg acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt
    18481 tgatgttcat ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt
    18541 tcatatcatt cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt
    18601 aataaatctt gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga
    18661 agatgtgcct cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga
    18721 ttatcaagca gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg
    18781 ccatcttctg gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca
    18841 tactgctcca cgcgctcgat catagctttg atataacttt cttctatttt attttctgat
    18901 aatagcggct gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat
    18961 tggatcattt cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct
    19021 aaaagtttgt tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct
    19081 tttaattcga tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat
    19141 cctggtatca gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc
    19201 atgattggat gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta
    19261 gaaaaaataa tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc
    19321 tctgtaaaaa agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag
    19381 ctaatgccac ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc
    19441 gcttttctga tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg
    19501 ctcttcccta ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa
    19561 gcatattgat gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata
    19621 cgaaaatagg cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca
    19681 aattctacgg ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa
    19741 aaatcaagtg caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa
    19801 aatataatct ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta
    19861 atgtcagtga ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt
    19921 tttccaattc gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt
    19981 gcgaatcgaa gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa
    20041 taatcaatgg aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc
    20101 atcaaacgag cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc
    20161 ctaaaaccag ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt
    20221 atctttttga tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga
    20281 aaaacagaaa cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct
    20341 tgttcggtat acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt
    20401 gctcggaatt tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat
    20461 tgtttgatcg cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt
    20521 tctcttggtg ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac
    20581 ttatattcta gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg
    20641 atttttttta cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg
    20701 accaaatgtc tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt
    20761 ttttgaggtc agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt
    20821 ttcaaagtga ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa
    20881 agctgtttct ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc
    20941 ttctgtttca ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat
    21001 tgaacctctt aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag
    21061 tagttctata ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat
    21121 tcttacaaat atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca
    21181 aaattttcat ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta
    21241 atgtctcgta aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt
    21301 cttcatgata actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat
    21361 cgataccaaa gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat
    21421 cttttgtttg tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt
    21481 tgatgcggaa ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat
    21541 cgaagtaatc attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat
    21601 ctggttcgcc gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat
    21661 agctgttcgc agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat
    21721 aatgcatttc tacttctaaa atatccccta attttccgct ttcgattact ttttggactg
    21781 tcaagaagtc agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt
    21841 ttgctaatgc aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta
    21901 gtacattctt accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg
    21961 tagtgatgac aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat
    22021 attcgatgtc ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat
    22081 tgattttgtt cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat
    22141 tgccaagata agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata
    22201 tcctagaaaa gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac
    22261 acttgttatc attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga
    22321 tagtagcgga accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt
    22381 gaagaaaaac taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac
    22441 atccgtagga atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg
    22501 tatacttccc attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa
    22561 gaattccgtt tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg
    22621 attgatccta attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg
    22681 gcggatttat cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta
    22741 gatcaaatcg gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat
    22801 tctcaaatca cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc
    22861 atggctgaag aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc
    22921 gccattttta ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg
    22981 aagcatgtag aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa
    23041 caggctgata tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact
    23101 ttctcctctc aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct
    23161 caaaattttg aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc
    23221 cttcttaaag aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag
    23281 tgtttgatag gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac
    23341 ctcagcctgt ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct
    23401 aacttctgtt tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca
    23461 gtatacatgc agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta
    23521 cttctttttg aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat
    23581 gactgttgac catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga
    23641 ttctcagatc ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga
    23701 gtgtccgtcc attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg
    23761 tgaacaactt tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc
    23821 cccccgtagg ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat
    23881 tcgtttcatc aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg
    23941 tatgtttctc aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat
    24001 ctgcacctgg atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt
    24061 ggattttagc catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga
    24121 tctgctgatc cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta
    24181 ttcctgagag aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca
    24241 tttgtgttct ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc
    24301 cgctgctcct ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca
    24361 aagtacagtc cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc
    24421 ttaccgcctg ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc
    24481 aactgtcctg ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat
    24541 ggaacatatt tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct
    24601 gtcacatcga ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat
    24661 acccagctga cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct
    24721 tgatcacgaa cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct
    24781 actttaacaa atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt
    24841 gtatgcccgt tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg
    24901 ttcaaatgca tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa
    24961 ttcggatcaa ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg
    25021 tcgatttctt tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca
    25081 aatgttaaag cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt
    25141 ccttctttat cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca
    25201 ccgattgcga catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca
    25261 tttttgatgg tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt
    25321 gtttgctttt caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt
    25381 ccaacttcta aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca
    25441 ttgttgccga tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt
    25501 gtccaagtgt acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct
    25561 tcttctttag aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt
    25621 tttgtaactt tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta
    25681 tctagttctt ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca
    25741 gggaaagcta aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc
    25801 gtgtagactg catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca
    25861 gcatctgtcg ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt
    25921 gctgcatcca cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa
    25981 actgaaaatg aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg
    26041 ctgttttgga ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga
    26101 gctgctgtct cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa
    26161 ataaggaaaa ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc
    26221 ctgttctttt tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc
    26281 ctaaaagtgt atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt
    26341 tattgactac tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg
    26401 taaacggaat ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc
    26461 gataacttcc tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt
    26521 gaagcgcatc tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat
    26581 ttcctgcttt gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg
    26641 tctcgatttt gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac
    26701 caaaaccaaa atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc
    26761 ttagctgcat ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga
    26821 tcgaaaataa aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct
    26881 tttctccatt cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac
    26941 tatctgacaa ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg
    27001 aataagtgat tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa
    27061 aaggtggatc agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt
    27121 tttgattgtt ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga
    27181 cgacttctgg tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt
    27241 tagcggccag agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt
    27301 ctgcggtaaa cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta
    27361 ccgttttgcc cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg
    27421 tgacaccatt taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat
    27481 gatttttcgg cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt
    27541 ctgtttctgt ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac
    27601 acatactaag ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa
    27661 aagataccaa atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc
    27721 aattcctccc gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg
    27781 gttgtttttg gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga
    27841 cccatcttcg tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt
    27901 aaaagtttcg gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt
    27961 ttcttgtcca tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc
    28021 tccttttaat gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag
    28081 tcgattttga tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta
    28141 aatgattttt ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc
    28201 ggcaggacca gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc
    28261 aagacctttt tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt
    28321 tttctgtagg gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc
    28381 atacttacgt actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt
    28441 atcatcggaa gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt
    28501 gatttcccaa gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag
    28561 cgtataggtc atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc
    28621 atgatctgtt agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag
    28681 atccttttca cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt
    28741 agtatttttc catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc
    28801 tttgattgtt tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat
    28861 ctcttgataa ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt
    28921 atttgttgta tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc
    28981 atttgtcgta tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt
    29041 gttgtcaaat tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga
    29101 tggtataccg aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc
    29161 gttcatttga taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg
    29221 gtaatggatt tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt
    29281 tccttctatg gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga
    29341 aagtgtacca ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct
    29401 tacagttgct gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg
    29461 atcagctgat tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg
    29521 agactctact tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag
    29581 accatgtatt tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa
    29641 cgtactgtcg attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat
    29701 tagagaagta tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct
    29761 gcttgattgt gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag
    29821 caaaacgatc acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag
    29881 tgctttttgt gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt
    29941 tttcactgaa tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct
    30001 tgagtagccg acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc
    30061 gacaaaacga tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc
    30121 tgaccagtct acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg
    30181 agcattcaaa taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat
    30241 cgcatagtct gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt
    30301 aggatctcca ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg
    30361 atgatcaaga acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg
    30421 atcatctgtt gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa
    30481 ggataatgta ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc
    30541 ttctatttcg tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc
    30601 aataatgtca tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc
    30661 tggatcgatt ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt
    30721 aattttcccg ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact
    30781 taagtaattt tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc
    30841 atttagcttg atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat
    30901 tgtttctaaa actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt
    30961 tgataaactc aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat
    31021 ttttcccctc ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa
    31081 acaggcgtta cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa
    31141 gggaatcaca ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta
    31201 atgagcgttt cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga
    31261 aggaaactat agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat
    31321 gactaaaaaa gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat
    31381 cgaagagatc aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg
    31441 gtatcgtctt gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca
    31501 aagttctaca aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga
    31561 gtattattgt gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg
    31621 caatcaaatt ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac
    31681 tagccttaaa agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta
    31741 cggaagacgc acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa
    31801 gaataattgt cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat
    31861 tatgtatttt gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc
    31921 tggagactct tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa
    31981 tctcttagga aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac
    32041 gattttttca gaattttcgg agaaag
//
LOCUS       DASJTC010000022        31347 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.022, whole genome shotgun sequence.
ACCESSION   DASJTC010000022 DASJTC010000000
VERSION     DASJTC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.022"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(471..2363)
                     /locus_tag="U4B19_000929"
     CDS             complement(471..2363)
                     /locus_tag="U4B19_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2076687.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2360..4087)
                     /locus_tag="U4B19_000930"
     CDS             complement(2360..4087)
                     /locus_tag="U4B19_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2076688.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(4103..4753)
                     /locus_tag="U4B19_000931"
     CDS             complement(4103..4753)
                     /locus_tag="U4B19_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN2076689.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4957..5253
                     /locus_tag="U4B19_000932"
     CDS             4957..5253
                     /locus_tag="U4B19_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2076690.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5445..6065
                     /locus_tag="U4B19_000933"
     CDS             5445..6065
                     /locus_tag="U4B19_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEN2076691.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(6157..7323)
                     /locus_tag="U4B19_000934"
     CDS             complement(6157..7323)
                     /locus_tag="U4B19_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2076692.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7335..7688)
                     /locus_tag="U4B19_000935"
     CDS             complement(7335..7688)
                     /locus_tag="U4B19_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN2076693.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7816..8838
                     /locus_tag="U4B19_000936"
     CDS             7816..8838
                     /locus_tag="U4B19_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEN2076694.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8857..9018
                     /locus_tag="U4B19_000937"
     CDS             8857..9018
                     /locus_tag="U4B19_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076695.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9277..9711)
                     /locus_tag="U4B19_000938"
     CDS             complement(9277..9711)
                     /locus_tag="U4B19_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076696.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9821..11680)
                     /locus_tag="U4B19_000939"
     CDS             complement(9821..11680)
                     /locus_tag="U4B19_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2076697.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11798..12958)
                     /locus_tag="U4B19_000940"
     CDS             complement(11798..12958)
                     /locus_tag="U4B19_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEN2076698.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13265..14689)
                     /gene="araA"
                     /locus_tag="U4B19_000941"
     CDS             complement(13265..14689)
                     /gene="araA"
                     /locus_tag="U4B19_000941"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEN2076699.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14705..15403)
                     /locus_tag="U4B19_000942"
     CDS             complement(14705..15403)
                     /locus_tag="U4B19_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN2076700.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15360..17021)
                     /locus_tag="U4B19_000943"
     CDS             complement(15360..17021)
                     /locus_tag="U4B19_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEN2076701.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17239..18639
                     /locus_tag="U4B19_000944"
     CDS             17239..18639
                     /locus_tag="U4B19_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEN2076702.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18891..19970)
                     /locus_tag="U4B19_000945"
     CDS             complement(18891..19970)
                     /locus_tag="U4B19_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2076703.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(20056..21015)
                     /locus_tag="U4B19_000946"
     CDS             complement(20056..21015)
                     /locus_tag="U4B19_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEN2076704.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSKKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(21027..22538)
                     /locus_tag="U4B19_000947"
     CDS             complement(21027..22538)
                     /locus_tag="U4B19_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN2076705.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22560..23123)
                     /locus_tag="U4B19_000948"
     CDS             complement(22560..23123)
                     /locus_tag="U4B19_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN2076706.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(23129..23992)
                     /locus_tag="U4B19_000949"
     CDS             complement(23129..23992)
                     /locus_tag="U4B19_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2076707.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(24006..24902)
                     /locus_tag="U4B19_000950"
     CDS             complement(24006..24902)
                     /locus_tag="U4B19_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2076708.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(25077..26366)
                     /locus_tag="U4B19_000951"
     CDS             complement(25077..26366)
                     /locus_tag="U4B19_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2076709.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26338..27855)
                     /locus_tag="U4B19_000952"
     CDS             complement(26338..27855)
                     /locus_tag="U4B19_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEN2076710.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            28053..28943
                     /locus_tag="U4B19_000953"
     CDS             28053..28943
                     /locus_tag="U4B19_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEN2076711.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(29016..29840)
                     /gene="nadE"
                     /locus_tag="U4B19_000954"
     CDS             complement(29016..29840)
                     /gene="nadE"
                     /locus_tag="U4B19_000954"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEN2076712.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29852..31324)
                     /locus_tag="U4B19_000955"
     CDS             complement(29852..31324)
                     /locus_tag="U4B19_000955"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEN2076713.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT         9134 a         6586 c         5460 g        10167 t
ORIGIN      
        1 attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa aaagtcgctt
       61 agtttcgagt agtaagaaaa attttgtgaa aatggctttc tacatttttg cataaatttg
      121 gcttaatacc gagaaactgg cttttgaaca ccgtttacca agaggttgtg agcctgccgt
      181 ttaactccga gtcacaagga gcaatttcaa aaaacagctt ctcaggtttg ttgactattg
      241 tgacttgtga ccgaggagtt ggcaggagaa caccgtttaa ggaagaggtt gtaaaatcag
      301 cggtttgacc tgagtattag agatacaaaa cggaaaaata gcttctcata ttttgccgtt
      361 ttttgagcta ataccgaagg acagctgatt gaacaccgtt tattcggaaa aagggactgt
      421 gacacgactt ttgtcacagc ccctaaaaaa aataataaat ttgtactatt ctatgaagct
      481 tcttcttcag aaaactgact gttataaaga tctgcataga atccgtcttt ttccatcaat
      541 gtatcgtggt ttcctgtttc tacgatcgaa ccatgattca tcacaataat gttgtctgca
      601 tcgcgtattg tagacaagcg gtgagcaaca acgaaacttg tccggttttc caataggcgg
      661 ttcatcgctt tttgaatcag tatttctgtt cgtgtatcta cactagatgt tgcttcatca
      721 aggatcagga catctggatt tgctaagaat gcccgcgcaa ttgtgattag ctgacgctgc
      781 ccttgcgaaa tattgcttgc atcctcgttt aatacagtgt tatatccttc tggcaattta
      841 cgaacaaaat cgtctacgtg agcagcttta gcagcctcaa tgatttgttc gtctgttgca
      901 tcgtcgttcc catatttgat attgtcatag atacttcctg taaacagcca agtatcttgc
      961 agtaccattg aaaactgtgc gcgcaattca tcacgtgaaa gatcacgggt atctacacca
     1021 tcgtaacgaa tactacctcc agaaacatcg tagaaccgtt caagaaggtt gatcagtgtc
     1081 gtcttccctg caccagtcgg acctacaata gcgaccatct caccaggttt gacatccaag
     1141 ttaaaatcag tcattagcaa gttatctggt gtgtagccga attgaacatt ttcaaaacga
     1201 actttgtatg gtgtatcttt ttcttctgga aggccagatt tttcattgga catctcttct
     1261 tcatccaaca cttcaaatac ccgttcggct gaagcaactg ttgattggat agtgttcatc
     1321 aaacttgcaa gttgagtgat tggttgagag aactgtgttg tatattgcaa gaacgcttga
     1381 acatccccta atgtcatgtt cccatttgca acttttactc cgcctagtac agcgacgaac
     1441 acatagccta gatttttaac gaaattcatc aacggcatga tgattgccga aataaattga
     1501 gctttccaac cagcggcata cagattctga ttttcttttt caaatacttc ctggtctttt
     1561 tcagtatgat tgaatgtttt aacaatggta tgaccaccat atgtttcttc tacttggtta
     1621 ttcaataaac ctaaactttt ttgctgatct gcgaaaaaca cttgtgattt cggcgctacg
     1681 atcatcacga tgatcaagct taaagggatc atcaataacg cgactaacgt caattgccag
     1741 ctgattgaaa gcatcatcac gatgacccca atcaatgtga ctgcactagt tacaaattga
     1801 gtcaaatttt gctgcagcgt gcttgcgatg ttatccatat cattgattgc tcgagacatg
     1861 atatccccat ttgtatgtgt atcataatag gaaatcggta cacgattcat tttggcttcc
     1921 aaatcatgac gtaattcata aactgttcgt tgggaaacac gtgtcataat aaattgctgt
     1981 aaaaagttga atacagcaga aatcaaatac atcacgatta ctgtcgcaat gatctgcgcg
     2041 attttatcaa agtctatcgg aaattttgtt acttgttggc cttgttgcat ctgttgagcg
     2101 ccagccatta cacctttgaa tatttctgtg gttgctttcc ccaagacctt tggtgtttgg
     2161 atttggaaga tcacggctgc tattgctagt acaaatacag cgatgatcgc aaccatacgt
     2221 ttagacatat agccaaacaa tcggcgtacc gttccccaga aatttttagg cttttcacct
     2281 tttacaccga gatttctgcc aggtccacca tgtcctggac cgcctgctgg tccatgagag
     2341 ggacgtgtgt ttgttttact catgctaagt cctcctctct tagttgtgaa tgaacgattt
     2401 cttgataagt ttcgtttgtt gctaatagtt cttcatgtgt tcctttgcca accagttttc
     2461 cttcatctag cacaaggatc atatctgcat ccattactgt acttactcgt tgagcaacta
     2521 aaacggtgat actttctttc atattcgttt tcaatgcttg gcgaagattg gcatccgttt
     2581 tgaaatccaa cgctgagaag gaatcatcaa acacatagat atctgctgtt ttgactaatg
     2641 cccgtgcaat tgccagacgc tgtctttgtc caccagagaa gtttcctcct ccttgttcca
     2701 catggctatc taagccatct tccatctctg aaacaaaatc tttagcttgt gcgatttcta
     2761 atgctttcca aatcatttca tctgttgcat ccggcttacc atactgcata ttgtctcgga
     2821 tcgttccaga gaacagaact gctttttgag gaacaaagcc cattaattct cgcaaattat
     2881 attgagtcat gtcactaatg tccgttccat ttatttggat cgaaccagat tcgatgtcat
     2941 ataatcgagg gatcaaattg actaaggtcg ttttacctga accagtcccc cctatgattg
     3001 cgacgatctc gcccgacttg gcttggaaat caatatcttc caatgcaagt ttttctgctc
     3061 catgatatcg ataattgaca tgattgaagg ccaatgttgc gttttttcca gcaaaagaaa
     3121 ctgttttagg attttcaggg tcatggatac cgattttttc atccaacact tcattaatac
     3181 ggtcagctga agcttgtgca cgaggcacca tgataaaaat agcggaaagc atcataaagc
     3241 tgatcaagat ctgcatcgca taagtcataa atgcaatcag attaccgact tccaaagtca
     3301 tatctgctat gtagtgtcca ccaaaccaag tgattgctac gtttgtaccg ctcatgatca
     3361 atgtcatcat tggcatcatc aatgcgacga tcgtattaac tttgattgcc gtatttgtat
     3421 aatctttatt cgctaaatca aaacgatttt cttcaaagcg tgtcttatcg aatgctcgaa
     3481 tcactctgac ccctgtcaat ccttctcgaa ataccaggtt taaacggtca gttttctttt
     3541 gcatactttt gaataatgga acagcaaagt acatgattcc gccgatcaaa ataatcatta
     3601 ctgggatgac aactaagaag atttttgtca actgatgatc tttttgataa gcaagtatgc
     3661 tcgcgccaat caacgtgatt ggtgcattga tcatcatacg taaaaacatt tgtgtaacca
     3721 tttggatttg agtcacatca ttggtcgttc ttgtgatcaa agaggccgtt ccatatttat
     3781 caaacgcatc ttttgtcaga ctctctgatt ttttatagac atccgtacgc aattgcttac
     3841 ccagtttctg tgattcacga gtagcaaaaa atgtatttgc gatagcagct aagatactga
     3901 tcaaagaaaa gccgatcatc acaaaacctg tatgccaaat ataatcgaca tctccttgcg
     3961 ctaccccttt atcaataata ttggaggtaa gtgtcggcaa atataaatct gccagtactt
     4021 ggatcaccat gaaaactgca gctgcaattg ctgaagtcaa ggagatccgt tttacgattt
     4081 ttagcatcta gttcccttct ttctatttat atactccgtt tttcagccaa gaaagtttca
     4141 aattgaattc actgattgct tgctctactg agtaatcttc agatgttgaa aataagcgat
     4201 acgaatgcgc aattgttgta aatacagtat tcatcactaa ttggatcagt aattccaatt
     4261 catgatcatt ttttactttc agcttcgtcc gatcgatcaa ttcaagcaac tcttttgcat
     4321 cttgtttgtg ttctttatgt ggatcgtgag gcgttttttg gcgactcgta tgaggagcga
     4381 cttttcttga ggcacggtaa tccatgttca taaacaagtt acgataaaaa gaagcattct
     4441 cgccttccaa aatctcataa atcattttag aaaaatagga ttcgaatcct ttgaatagat
     4501 caccatcgga ttctttcatt agttgtaaca tatctttaga gctgtcacgt ctcattgtct
     4561 caaagtaata aaaataaaga tcctctttat cttcaaaata ctgatagaaa ctgcctctag
     4621 ggatatccgc taatttgacg atattagcaa tcgaagcttc ttttaagggt actctagaaa
     4681 actcgattct cgcagcctcg attagtcttt tttgtttttc tttaggcaaa tggaaaaatg
     4741 tctgtgtcgg catatggatt ctcctttctt tcaagatgac acattgtcat atgacgctat
     4801 gtcattataa cacctcctac ctgttttgca agacatttgc atagtttttt tcattatcat
     4861 ccgatacatt aaaaaagtga taagaggaat ccttccattt gaattaatga gagttgccca
     4921 ttaaaatagc cacatactaa acaaaggatg agataaatgg cccaatcaat tagtgtccaa
     4981 gacttcaata aattgcctct cgatgctgac agcatcgttt tagatacaag aaatacctta
     5041 gattacacaa aaaaccactt aagccactcg ctttctatcc ccgctgctat cttgccaaga
     5101 caattaaaaa atctagatcc aaagaaactt tattatattc taagctatac aggcaggcga
     5161 tcagaagcat tagccgatca acttactgct aatggttttc gtgctgttta tatcattggt
     5221 gggatggaag ctcttcagtc gagtgttgct tgaatattgc ttgttagatt caaaagaggc
     5281 tgtgacaaaa aatcgtgtca cagcctcttt tataccctgt ctttttttag ttatttattc
     5341 ttcaacctta tcaatatgac ttgatagcct tttctttttc gagtaaactg attttgttag
     5401 tcatagttat aacaatcata gcaataaaag gagaaggtaa gcatatggca tctgttttag
     5461 tagtcaaagg tcatccccta actgcagaag aatcgcgcac agttaaggca ttgacgtctt
     5521 ttttaacaag ttataaagaa aatcatccag atgatgaagt gactgttttg gaactttatc
     5581 gcgatgatat tccggaaatc gatgaagaat tattatcagg ttgggaagca ttacgtgctg
     5641 gtgcagaatt cacttcctta tcagaaagtc aacaacaaaa aattgctcgt ttcaatgaat
     5701 tgactgatca gttccttgct gctgataaaa tcgttatcgc aaacgcctta tggaatctaa
     5761 atatcccaac aaaattaaaa gcttggttcg ataccgtcaa tgtagctgga aaaacgttcc
     5821 gctatacaga aaacgggcca gaaggattag tgacaggtaa aaaagcactg cacatccaat
     5881 ctaatggcgg tgtatacaac ggacaagact ttgcctctca atatgtaaaa ggtattttaa
     5941 actttgtagg catcgatcaa gtagatcagc tattcatcga aggaatcgat tatgaccctg
     6001 accgtgcaga tgagttgatg caaaatgcat tagataaagc tgctgcttta ggtaaatcat
     6061 tttaaaaatt gagataaatg catacaaaag agacagagga aaaaatcttc aaagcagcat
     6121 ttgtgccttg gatattttcc catctgtctc tttttgttat tctttctgct ttttcaacgg
     6181 attttcttca taaagagaag agattgttgt gtaccattca aaaacatgag taatcactac
     6241 tttagctgct gcataaacag gaatccccaa gatcacaccg atgattccga acaatttccc
     6301 agaagtcagc agaactaata aaatcgttac tggatggatt gctaactgat tccctagtac
     6361 taatggagag atcaagcgtc cttctattgt ctgctctact gcaaaaacga tcagtacttt
     6421 gatgatcatg aatggacctg ctacaatggc taaaaagata gccggaatca tcgctaaaaa
     6481 tgatcctaag taagggatca ggtttaaaaa acccgcaata atccccaacg tgacagcata
     6541 ctctaaacca atgaccgcaa aaccaatcat aaacatgatt gccacagcaa aagccacagt
     6601 tagttgtcct cttatataag aagaaacttg actattcatt tcactcagca cattcaacgt
     6661 cggtttttgc atgcgtgtcg gtaaaaattt gacaaaataa ggtgctaatt gtttaccgtc
     6721 tttcaataga taaaataaga taaacggcat cgtcagcaga gccactaaaa tagtcgctac
     6781 cgcgccgaca aaacttccaa gactctgaac tgtactctta gatatatctt ggatcatatc
     6841 gcctgccgaa ttcatgaact tatcacctgc tgtttccaac tgttcgcgaa attggttgaa
     6901 cagcgggtcc cgcaaaatct cggttaattt attatcaatc gtatctacat attgagggaa
     6961 gttactgatg aagctcactg tttgttcctg aatcttaggg atgatcacta cgctccccca
     7021 tgcgattagt gcaacaacca aaacaaacag cccaatgata ctgtaaagcc ttggtacttt
     7081 tcgcttttca aagtaatcaa ctaccggatt catcaagtaa tacaagattc ctgctaaaat
     7141 gataggcaga cctacgattg ctaaaaattg ccagattggt gcaaacaaat atgaaacttt
     7201 tgtaaataaa aagacgatca gcaatattaa caatacaaca agtaaggctg tgactacttg
     7261 attatttaaa aaccagcgcc aaaaccacga taccggtctg ttcttcttat tttttccttc
     7321 cataattcct cctgttagtt ataaaaaatt tggcttccca aaccaatagc aggcagtcta
     7381 tgcggatgaa gatagattcc atttgcaatt tgtccttctt ctggacaagc atcaaaaatc
     7441 aatgatacgt gagctactgt ctttaaattc ccattggcca tcggaccgac ataatcgatc
     7501 gtatatacct gatcatcaaa agcaattgtt ccgcctgctt tcaaagaaaa aggttcgtct
     7561 tttgatactt cttgaatcac cgaatatttt cgtaaggcag ctgttgctgt tttatcgaac
     7621 aaaatcagca tgggttcttc cttgtttatt gcttctgtcc caatttctgt aatctttgcc
     7681 tgaatcattg cactctctcc tttaattgct ttcaaaaaca gtataacaca ttctcattct
     7741 ttagccgtgt ggaataatta tgtgatagaa taaaaacgaa aactatttca ttacaattag
     7801 gaggaatcag ctcatatgat cgaaaagata attttaggaa cctatacgcg tcgcgaaagt
     7861 aaaggaatct atacgatcga ccttgataca gaaaaagaag aattagccaa cctttcatta
     7921 gctgttgaag aaaatagccc tacctatgta gcaaaaagta aagctggcaa tctttacaca
     7981 gttactagtg tggatggatt aggtggtgcc ggtgcgtacg ataaggattt tcattttttg
     8041 aatgcggtaa cagagtctgg cgctcctttg tgttatgtag cggttgatga agcacgtcaa
     8101 ttactatacg gggcgaacta tcataaaggt gaagtaaatg tctaccatat attaggagat
     8161 ggcgaactga aagcagcaga ttcaatattc catcaagaag cgacaggtcc tcataaaaac
     8221 caagatcatg cgcatgtcca ctatacggat ctgactcctg accaacgatt agtcgtatgt
     8281 gatcttggta cagatcgtgt gtatacttat gatgtgtccg aaaacggcaa attgaatctt
     8341 gtaactacct tcactgcaga gccaggaaca ggccctcgcc atcttgtctt tcatccaaat
     8401 ggacaattcg cttatttatt tggtgaatta gacagcacag tccgcgtgct ttcttataat
     8461 aaagaaacag gtgcttttga agaattacag aaaatatcga ccttgccaga agaattcgac
     8521 ggtgaaaatg gaggggcagc tatccgcatt tcaaatgatg gcaagttcct ttatgcttct
     8581 aaccgaggac ataactcgat tgctgtattc gcagtttttg aagatggttc ccacattcaa
     8641 aacaagcaaa tcatctcaac agaaggggat tttccccgcg atttcgcact agatccaagc
     8701 aataattttg tcgtgtgtgc caatcagaac accgataatc tgacattata tcgccgtcat
     8761 cctgaaacag gattactgga attactgaaa aaagatgtct atgtaccaga atgtgtctgt
     8821 gtttattttg atcgttagta agaaaaggag aatgaaatga atcagcaaga cgtattagac
     8881 agactacggg aagaactaaa tattcctttc tttgatggga aaatcgaaga taaagagtat
     8941 agcgaagagg aatatcagaa actcaaagcc gatttacaga actactttga acaatatgta
     9001 cagaatgtag aaaactaatc agctttccat actatctgaa aagaacatca ggacaagact
     9061 tttccgtcta tcctgatgtt cttttctaca aataaaaaac cgccgagatg gttagactcg
     9121 acggaaaagg agtatttact cgattttgag taagtacaag gttgttgttg gttataggta
     9181 tgtcattatc ataaaggatt ttgatcactc tatcataaag gtaatattac atttatatta
     9241 gataactcta gtttttattg ttatcaaaga tttcttttat tttttcaaca acaaattagt
     9301 cattttttgg atggcagctg tcaattggtc tgtcgcttgg tcttgttttg cttcagatgt
     9361 attgaataga tcttcatcta cccctgattc aagatcgata ctttggatca tttcttcaca
     9421 gcctgctaca tatgaaagat aagctttttc aaaattttta tgaatgccca ttgcttttgc
     9481 tggtggacgc aaagtactga ctttcataag cattgcccga tatttttttg ttccttcttc
     9541 aaataacagg atgatctctt caagagtcga tttgtccagt tcacttgttt ttccttcatc
     9601 aattgcgttt ctgatcactt catatttcgg atgcattcct tctccgattg cttctgtttc
     9661 cttgacgatt tgatcgatgg actgcacata atagccaata ttcggtaaca tgttcgtttt
     9721 cctactttct ccattgttgt tattatttat tctacttgtt tttatcctca aatgacaagt
     9781 acttgaaaaa atttaatggt ttctatacag ctcgccattc ttattcgtgc tcaaagaaac
     9841 catctgctaa actatattgt ttccctgcga caatgtcata ttggatcaat tgatattcgt
     9901 cgtaaatctc ttgttgcgag cgattgaatt gcgcttcttt ataccactgt ccgttttgat
     9961 agtacagttc tcgttcaaac gccggaagtt cctgttctaa tgataaaagc agctgataaa
    10021 agccatttgt tgtctgattc gtctgttcca ttagattcgc tgcaaaataa aacggactgg
    10081 tgattgcatc gtgagtcgtt tgcttttcta attctccttt actgtcgaac ataagaaatt
    10141 gtgtttcatg caatgtttgt ttgtcatttt ttgcttggat cgtatctgaa tagatccctg
    10201 gaagatgatc cccccaaaaa acaactaaag tccttctaga aagattcttc aattcttccg
    10261 tgaactgctt cagtgccgtg ctgctatagg aaatatcttg cagataattc tctaagtcaa
    10321 gtgtcccact tccatctgaa gtttttgcag gatagtctag attatcatac tttccatcgt
    10381 agggcatatg tgtctgcatc gtgactaaat gaatgaactg aggtgttttt tcttccttga
    10441 gaagtgtcat tacttctttg taagctgact catcagaaat atatggatta ttctctatcg
    10501 tatccgtata ggtcatcgta cgctcgctga tgaactggtc aaatcctaat gtctgataaa
    10561 catcctctct tttatacatc gaagtattgt atggatggat cgctgtcgtc tggtatgatt
    10621 gtgcgtttaa cgctgaaacg atagaaggaa gctgatccat ttttggtaca agcatcgtgt
    10681 aaggggtggt gagttgcgca ttaaataacg ccatagaaaa accagtcaat gcttcaaatt
    10741 cgatatttgc tgttccaccg ccatagtttt gagaaagcat attgccacta tatgtttgat
    10801 cagctacttc ataataatct gccaatggct caccagatac ttctacgccg tttaatcgac
    10861 taggatctga gaaactttca ctcatcacga acacgatgtt cggcgactct tcctctacag
    10921 ttttattttt ttcatcggct agcttctggt atttttcagt gatttctttg atcttttctt
    10981 tcgaatatcc ttcaggttca tccattggtt ctaccttcag attgtacaaa aacccaccga
    11041 taaaaccagt gttatagtag ttcattttct ggctgtaagg tatccatagt gccgttttat
    11101 tatatgcttt tcgcaacaaa ttatcgggat tgttgaaatt actgatatac cctagcagtc
    11161 caattgccac aagcagtgtc gttaatcgga tcaattggaa tcgccttcct tttttcaggc
    11221 ttcgaaacat gtaccagcaa aacaatccta tcacggttcc tgcggcagct aagatcactg
    11281 taaacagcat agtgccggtc atatctttca gcaatccgat ttcagtgatc atcttcaaat
    11341 cgtctggata aatcggttct tgccgataac tcattttcat ataatttgca aaacctaata
    11401 caccgatggc tacgacatac gtaagggaac ctaaagggac agatcctgct agtgcaatca
    11461 gaaaaataag aataatcagc aagacgagac aggctaagaa gaatttttcc gtatgccaag
    11521 aaaaagcaaa tttcaacgca aggtctacgg atagatcatt ttgacaccac tgcaaaaata
    11581 aattactaaa aacaacaaga aaaaagatac tgatgattgc tgctgttcct tttaaaatct
    11641 ttttctgttt ggtcgaaagt ctgcttatat atcttttcaa atgcttgctc cttcatttct
    11701 atcaataaaa gaatgttgct tctcttattt tagttgtacg aaattcgaaa gtaaagaacg
    11761 gaaagaagaa ttaatgaaat ccaaatattc tctttcttta tggcctttct ggtttcttta
    11821 ataagcggac ctttcgtact ccagaaattt tttctagctg ttgtttcact tgattgagct
    11881 gttcaaaatc agtactgtct atgtcaatca atatgtagac atgctcattc ttatggttac
    11941 gcgatatatc tgcgatcgct agctcgttct cattcagcag agaaaatatc tctgctaata
    12001 tattggtttt cttttgataa aatatagtga atcgaaacgg cgcttggaaa aacattcgtg
    12061 cggatggata attgaccgct tctctaaccg taccgaacaa aagataatct tttaaggctt
    12121 caactgccaa acgtccgctg tctgccaaag cttcctgagt cgatccccct agatgaggca
    12181 acataagtat acggtcattt tctaagaact cttcttctgg aaaatcagtc aagtatttag
    12241 ccagatgttg tttttccaat gcttgcataa cagcatattt atcaacaatt tcacttcttc
    12301 ctacattgat aagtacagca tcttttttca ttttttctat attcgcctga tcgatcaatc
    12361 cttttgtatc ttctgtcaac ggcagcatga ctacgatgta atccgaggta gaaagaactg
    12421 tttctaaatc tgcttgccga acataatcca actgggcatc gcggatagaa taacccaaga
    12481 catccatccc taagctatag caacttagcg ctacttttgt gcctatcgct ccaagaccaa
    12541 gaagaccgat ggttttgcct tgcaattctc gtcccacata agcacttctt ttgttttctg
    12601 cctgttctaa aatattagga cctgttagtg tttgtaccat acgacttgct tcgattatcg
    12661 gacgggaaga taagagcaaa cagcacaaaa caagctcttt tactgcatta gcatttacac
    12721 ctggtgtatt catgacgatc gtcccgtttt cagaagcttt ttcaacgttg atcgtgttca
    12781 ccccaacacc agctcttgaa attgctagca aatctggtgt aagccattca tctttcactt
    12841 tcgtactacg aataactatt ccttcggctg gcaaatcatc tattattttg aaatattccc
    12901 cttgtaactt ttctatctct tgttctggaa tagcatcaat caaatgaacg tgatacataa
    12961 aaccccctcc ttactaacaa gtatctcgaa atttctgcaa ttagacaact gtttaccatt
    13021 tgacttccta gaagcaaaaa gagcagttag agcagagtag gctgagtgat aaaaataagg
    13081 ctatgacaag ttactgccat aagccttatt ttcgagagca aaatattgag ggtgagatat
    13141 cttgataaat ggtgttccgt tagctgacat tgataataag actaaatttc aaaaaagttg
    13201 aaaagcaaat ttcgtcaatc tcttcttatt tcttaaaact aagtggcttt ttcacaacct
    13261 ctttttatct aataaccact gtttccaact caactaattt tgcccacgtt tcaatttgat
    13321 ctgttgttag atttagcgag acaacggtat gatgaccgcc acctgcctga atccatgaat
    13381 ggacaccttc atggaaattc ggtttagttt tccataacac acgtgcaaca ggtaaattag
    13441 gtgcttcagt cgttggttca aaggcttcta cttcattgat cagcagttta taatgcgtac
    13501 cgaaatctgc catcgatacg actacccctt ctcctgcttt cccatcaaat acaagacgcg
    13561 ccggatcttc tctatctccc attgataaag gagaaacaac gattcttggt ttcgtcgcag
    13621 caagcgtcgg atctacttcc atcatatggg attccaagat tgcttcttgg ccactagcta
    13681 gctcgtaagt ataatcttcc atgaatcctg tatctttccc tcgagccatg attttcagca
    13741 accgatcaat cgctgcagtt ttccagtcgc cttccccagc aaaaccgtat ccttctgcca
    13801 tcaaacgttg tacagctagt ccaggtagtt gtttcattcc atacaggtct tcaaaattag
    13861 tcgtaaaagc gttatagcct ccttcttcta agaaacggcg aataccgatt tcctgctgag
    13921 cttgtacttt tacatgttcc tcccatgtct tttcttcata atcaccatag tcgaaggtat
    13981 acaattcctt gtactcttca aataattgat cgacttcttc gtctgatacg cgatcgatta
    14041 cttgtacaag atctccgata ccaaaataat caacagtcca accaaattga atctgtgctt
    14101 ccactttgtc tccttctgtg acacccacgt ttctcatgtt gtcaccaaaa cgagccactt
    14161 ttattccaaa actttcatta taggctactg caacatccat ccaatcagca atctcttttt
    14221 ggatctcagg tcgtttccag tagccaacga cgattttatt ttgtttattt aatcgagcat
    14281 tgatgaaccc atattcgcgg tcaccatgag cggattgatt taaattcatg aaatccatat
    14341 cgatcgtttt ccaaggaatg ctttcgttat attgcgtagc taaatgaagc aatggttttt
    14401 gtagaagctt tgtcccgcgg atccacattt ttgctggtga gaaagtatgc atccaagtga
    14461 tcacacctgc tacttcttct cgataattga cctctttcat gatttttgtg atcgtatcag
    14521 cagtaactgc taattcttga aggcggattg gataaggcaa ttggccattt tcattcagtt
    14581 catcgaccat ttcttgtgca tgtttcttta cttctcgcag tgcttcttcc ccatataaat
    14641 gctgtgatcc tacaacaaac caaaattctt tttctccaat attcaacata attagccact
    14701 ccttttattt gttttgtccg taatacgcat tttttccgtg ttttcgtaaa taatgtttat
    14761 ctaaaatccg ttgaggcagc tcttctgaaa atgaattgag ctgtctggta aataaattca
    14821 tcttgcatac ttcatctagt acgactgcgt tcattaccgc actttgcgca tctttccccc
    14881 aagtaaatgg accgtgtccg tgtaaaagta cccctggaat tgctaaggga tcaattttgc
    14941 gttctttgaa tgtttctatg ataacttttc ctgtttcata ttcgtacccg ctgtcgattt
    15001 cctgttgtgt caaaaatcga gcacaaggta ccgatccata aaaagtatct gcatgcgtcg
    15061 tccccatcgc aggaacatcc aatcctgctt gtgcccagat cgtcgcccat gtcgagtgag
    15121 tatggacgat tcctccgatt tgaggaaatg aacgatataa aacagcatga gtaggcgtat
    15181 ccgaagaggg attgagttta ccttcaatga tatgattgtc caagtccaca acgaccatgt
    15241 cgtctgctgt catctcttca taactgactc cgctaggttt gataacgaaa tatcgtgttt
    15301 caggatcaaa agcgctcaca tttccccatg tatatttgac gagtccgtgt ttaggcaagt
    15361 caagatttgc ttggtatact tcttctttca gttgttccag cattttctat cactcctttt
    15421 tcttgcaatt gatgaatcgc tgttttttca atgtcgatcc cctcacggta acgtctcata
    15481 aatatctgat agcctgctat ttcttctgct gaaggtgtga tggtgattcc ttggtcttgt
    15541 ccaaacacgt cttctgccaa gaagttttct aaagacttcc catgtcgatc catcatatag
    15601 gctgctaata acgcgattcc ccacgctcca ccttctccgg ctgtgtccat cactgtaact
    15661 ggtgcctcca tagcagatgc tagaatccgc tgtccaactt ctggtgtttt aaaaatcccc
    15721 ccatgcccca ctaaacgatc aatttggaca ttctcagatt tcaatatatc cattccgatt
    15781 ctcattgcac caaaagcact ggctaaatgg atccgcatga aattcgctaa atcaaaacgg
    15841 ctacctggtg tacgtacaaa taatggcctt ccttcattta ctcctgtaat gttttcaccg
    15901 gaaaaataac cataagaaag taatcccccg caatccgtat cgccttctag cgctttgttg
    15961 aataataccg tgaataattt atctctgctt atctccatac ccaaagaatt ggtaaattct
    16021 tcaaaaatac tgacccaagc attgatatcc gaagagcagt tattcgtatg aaccatgcct
    16081 actagttctc ctgaaggtgt agtgaccatg tcgatttccg gatggacatt ttctagttct
    16141 ttttctaata cgatcatagc gaaggcggaa gtaccagccg aaatgttccc tgtccctttg
    16201 gctacactgt tcgtagcgac cataccggtt cctgcatccc cttctggcgg acaaaccggg
    16261 attcctggtc tcaaatttcc cgtagggtcg atcagataag cccctgattc gcttaacttg
    16321 ccagcttctt ctcctgctac tagaacttta ggcaaaatgt gatccaagtc aatcgctacg
    16381 tgcgcttttt cgattaactg accaaaaata tttttcattt cttgatggta ggttttcgtt
    16441 gaaggatcga taggaaacat gcctgatgca tctcctacgc ctagtacttt ctcaccagta
    16501 agctgccaat ggacataacc tgcaagcgtt gtcaaaaaat taatttcttt cacatgttct
    16561 tctttattca aaatagattg atacaaatga gcgatactcc atctctgcgg aatattgtat
    16621 tgaaattttt ccgttaatat tcgttctgct tcttcagtga ttgtatttcg ccaagtacga
    16681 aatggtacta gtaactgttc cttgctatca aatgctagat aaccgtgcat cattgcactg
    16741 aacccaatcg acccgattgt cgtaagattc tcgccataga tttcgaatac ttctgctgcc
    16801 atttttcggt aactgacgcg cagtcctttc caaatatctt ctaatgaata ggtccagata
    16861 ccattttcta gtttgttttc ccactcataa ctaccagcgg caataggagt aaaagatgaa
    16921 tcaatcaata cagctttgat cctagttgat ccaaactcaa tccctaaaga tgtacgacca
    16981 tctatgacat ctaacatact ttgcgcattt acttccttca taatgattta actccctcct
    17041 tctcgtcaaa cctgatcaaa ccgtcttttt agtagcgctt acaatgatta ttgtatactt
    17101 ttgtacgtac atgtcaagca gttatttaca agaatacaat aaaaaacact tgttttgtac
    17161 gtacataagt tataaaatga aattgcttac aaaatctgat caaccgcctt ctcgaaattc
    17221 tagaaggaga gaatacttat gaaaaaagaa aaaaaagttt cctcatcgtt tatttatttt
    17281 tttggcgcat ttggcggcat tctatttgga tatgatattg gtgttatgac cggcgcctta
    17341 ccttttttac agcatgattg gggtttggca ggtaaagctt ctttgatagg atggatcacg
    17401 tcttcggtca tgttaggtgc gatccttggc ggctctttat ctggtcttct ttctgataag
    17461 cttggacgac gaaaaatgat tcttctttct gcacttattt ttatggctgg ttctgtcctt
    17521 tctgcatctg cccctcacaa tggttcttat tttctgattg cagcaaggat tcttctaggt
    17581 ttagctgttg gcgctgcatc tgccctagtt cctgcttata tgtctgaaat ggctcctgct
    17641 agactaaggg gaagattgtc agggatcaat caagtgatga ttgcttctgg catgctcctt
    17701 tcctatgttg cagattattt gctaaaaggg ttgcctgaaa cgatggcttg gcgcgtgatg
    17761 ttagggctcg ctgctgtccc cgcgctgatt ttatttttcg gtgttttagc tctaccagag
    17821 tcaccgcgtt ttcttatgca atctggccgc ttggaagagg caaaaagagt attgaactat
    17881 attcggacac caaaagaagc tgaacaggaa ttcgaacaaa tccagctgaa tgtcaaacaa
    17941 gaaaagacaa ccggaacttc ttggcatact ttgtttttag aaaaatatcg ttccttagtc
    18001 attgccggta ttggtgtagc agtattccag caatttcaag gagctaatgc tattttttac
    18061 tatattccgc tgatcgtaga aaaagcgact gggcatgcag cgagcgatgc attgatgtgg
    18121 ccgatcatcc aaggaatcat tttagttgct ggttctttgc tctttttggt tattgcagac
    18181 aagttcaatc ggcgaacttt attgaaaata ggcggtagcg tgatgggact ttctttcatc
    18241 ctaccagctg ttttaggaac tgtactggat gctcatacaa attcgttgtt gattctcttg
    18301 tttttatgta tttatgtcgc attttattct tgcacatggg ctccactgac atgggtaatc
    18361 gtaggcgaaa tattcccact agctgtccgc gggcgagcat ctggcctagc ctcttctttt
    18421 aattggattg gttctttcct agttggcttg ctttttcctg tgatgactgc ttctatgtct
    18481 caagaaatcg ttttcggcat ttttgggatc atctgctttt taggggtttt atttattcaa
    18541 gagatcgtac cagaaactcg tgggaaatct ttagaggaaa tcgaacaaag tgcatctaaa
    18601 aaaacttatc ctaaacgtat ttctatccat cattcttaaa gaggttgtga cagaagtggt
    18661 cagcttcaag aaataagaag gaaccgctta gtttccagtc acaaggaaca atttcaaaaa
    18721 acagcttctc atgtttgatg aatattgtga cttgtgacca gttggtagga gaacaccgtt
    18781 tagtggagag gtggtgaaag aaacgttcag ccttgagcga tgacaaaagc tctcacaaac
    18841 attgcgacga gtaaccgtag gagcaataag aggcggtaaa aacccgtctc ttatttttcg
    18901 ttttctttca ctgtttcccg ttcaactagt acaggttcat agagaatatt tggctgtgcc
    18961 tgtccttttt caatcgttgt gacgatccac cgtgcggcat cttgtcccaa cttttctttt
    19021 ggatgcgtca atgttgtcaa ttttttattc cctaaatggc ttaaagaaga atcgtctgtt
    19081 cctacgatcg ataggtcatc tggaatatga tagccatttt ctgtcaactt atctagcatt
    19141 aaactagcta tttgatcgtt atagcaaatg atgccatcta aagcttgttc ttctttcaac
    19201 cgttgtactg ttttttcagc gattgtttct cgtgtttccg tggtgaaagt aatgatatct
    19261 tccgtcaaaa aacgcacatt atactgttca cacgcgctga taaaaccttt catcctgtat
    19321 tttccttgaa gatcgtcgat ttttgtgatg aacaacagac gctgatgtcc tttttttatc
    19381 aaatattctg ctgccataaa accgactttc acatcatcta aacaaatata agaggcattt
    19441 aactcttcat aagcagcatt gatcatcacc atcggaatgc cttgttctct caaagcaaca
    19501 taaagtgcta agtttgggtt atactgatta cttttcgtag gctcaacgat caaaccatct
    19561 accccataag caatcatttt cttcaaacac tctttctcca attcgtgatc gttattcgta
    19621 ctggctaaaa gcaatgaata cccagattct cgtaattcat tttcgattcc tcggataatc
    19681 gacggaaaaa tataatcaga gagatatgtg gtgatgacac cgatcgtttt tgttctgttc
    19741 aatggtgttt tttctttctt atagctgtca tctacatatg tgcccgaccc tttttcttta
    19801 cgcagatagc cctcatttac caacacagcg attgcttgcc gaatcgtatg tctgctgacg
    19861 tcatatattt cttgcagctt gaattctgag ggaatggttg tatttacttc gtattcctca
    19921 cgaaggatct tttgttttat ctcttctgca atatcttgat attttgccat ttgccttgcc
    19981 cctttttatt tgtacgtaca tatatcataa cgagtctgca aggtttattc aagcaaaaat
    20041 caaggtttct tgtgctcata aggcatacgg aacaggttca ccaaattctg gaaatccttg
    20101 ctcattccac gtaaagcatt gcgcgttcgc gtgccgatta ggattgtcta acggatcccc
    20161 tttttggttc ttctctggtc gagcatgata gatcaaaata tcaaagcacc cgtcttctga
    20221 aaccgtaaac gagttgtgac caggaccaaa ttgcttattt tttttagaag aaacgaatac
    20281 tggctccgat aatttatgcc atgattcttt taataaaaga ttcttgtgct catcagccca
    20341 cagcaggccc atggcatagt tttcatctgt tgcactacca gaatacgtaa tgaatatttt
    20401 cccattgcga gtaaggactg ctggaccttc atttactgaa aaaccccgtt tttcccattc
    20461 atactcaggt atagaaagga ggatctgctt cccctttaac gtccaaggat tatccatttc
    20521 ggatagataa agattagaat tcccagtaat ttcaggatct tgttgggccc acacataata
    20581 tagctttcct tgagaagaga aagttgttcc gtctaaactg aaagtttccc ttttcgtttt
    20641 tatttgtccc ttttctatcc aatctgtatt cattggatct atatgctgat tttcaagtac
    20701 gaacatgcgg tgatgatgag attcatcacg tatagtttta tgattgcttg cagcaaagta
    20761 aatataccat tttccattga tataatggac ttcaggtgcc cagatcaatt cactcatcgg
    20821 tccttcttca tgagcctgcc atacaatcag gcttgcagca tgttctaatt cattcagtga
    20881 ctttgccctt cttaattcaa tcacttggta gcctggaacc gatcctgtaa aataataata
    20941 accatctgta tgtttatata cccaaggatc tgcccgttgc cgaacgatag gattattata
    21001 aagtgtcttc atcataacaa atttcctcac attttcttta ttctaattac attccaagag
    21061 tatttttcta tccctacatt taattcgtca tgtttttcta ttgaaaaata gtttatttca
    21121 ggttttattt tttctggttc ttcacgtgta tttttgtcca acagatcgct actggttaat
    21181 gtgtagtgct ctccttgatc tagcaaattg aaatcatgtt caatcgttat cttttgttgt
    21241 tcatttgtgc gattgatcaa gaaaatagtc agctgattgc cgttatctac gatcacactg
    21301 tccacataag gaacattttt tatcttttca caatcataaa cagggcaaat gatttgttgt
    21361 ttcaatactt gacctttagc atatttcgat aagtgttgca tcacataata agttggattc
    21421 ctccatgctt gtcctttagc atctgtcagt attaaaggta ttgtgtttac taacaatgat
    21481 tgacaactaa tctttacgcg atctgcgtgt cgtaaaatag ccagccctaa tgaaccaaat
    21541 aataatgtat cttccatagt aaagtaacac caatcgatgg gactaccgac ttcaaaatcc
    21601 ctatgtcttt tttccggttg ggcatgtaca ttccattcat caaacgctag atacattgtt
    21661 ttctcacttc tcaaatatcc ttttacataa tcacaagtag caacgatttc ttcaatttga
    21721 cgatcgaatc gataagcttt tgcaaggtat gtcgctgtat catcacgctc atttttagga
    21781 ggtttagtca agtcttcttc atcatactta tcaatatagt tatgcaaagc taaataatct
    21841 acattctcat aagcttcttc taatgcgata cgatcccatt cagggtacga tggtaaacgt
    21901 ggcgtagagc ttccaactaa gattgtttca attgaatcat ccactaactt catgactttt
    21961 gaggcttcat ttgctaacct accatattca taagcactct tagcccccac ttgccatgga
    22021 ccgtctagct cattccctaa acaccataat ttaacgccaa aaggcttttt ttgcccattt
    22081 tttattctta aatcactcca gaaggttcct tttggaaaat tacaatattc tactaaattt
    22141 tttgcttcat caattccttt tgttcctaaa ttcaccgtga atatcggttt cgcttgtaca
    22201 tgactgatcc atttaaaaaa ttcgtggagt ccaaaagaat ttggttctat acctcgccat
    22261 gctaaatcaa ttttttttgg tcgttgttct ttcctaccta cagtatccac ccaatcataa
    22321 ccagatgtaa agtttcctcc aggatagcga atgacaccta attctaattc ctttacataa
    22381 gagaggacat cttgtctaaa cccgtcacca tcagctgttt catggcccgg ttcataaatc
    22441 cctgtataga cgacacttcc catatgctca acaaatgagc caaacagccg agaactgatc
    22501 tctcctaccg taaaatcctc tctgattttt actttcactt cttcgtccaa cagctttccc
    22561 tacctttcat ttcctatagt caagataata gatacaagtt tccatacgcc accagcaaag
    22621 ataaaaataa aaggagggaa aagaaatatg agcagtacaa atcctaataa caatacgcaa
    22681 caaagaatat ttctcaaaaa atcaaaaaat actttccatg cactttgttc aaaaatttgt
    22741 aaaaatgtga tatctttgtt ttcagaaatc acatatatcc aggataaaag aaacatggaa
    22801 taaataagta aaactacata tccactaatt gaaagtacga ttgtctgttc ttctaataat
    22861 tgattaacaa gaaaataaat aatgatcgtt aacagactaa ggaacaagtt cttaccaaaa
    22921 tgttgcttta ttccttgaaa gaaaaaataa atgatatttc ctgatccctc acctttccat
    22981 tgcgccatta cataaaaaac agcacatgta gcaggcacga tagtaataac aggcaaacac
    23041 acgattaccc agagaatacc taataagatg taatcagaaa ccacaatcaa ctgtccataa
    23101 agtttagtct caactatttt catcttcctc actctttcat tccagacata gctgttcctt
    23161 gaacaaaata tttctgtgca aaaatataaa ccactagtaa tggcagcatt gaaataaccg
    23221 ttccagccat tacaggtcca taaaaagaaa tgttctgccc tgtaaataaa gctaaccctg
    23281 ctggtaaagt tctcattttt gaactacttg tcaatatcaa tggataaagc aagtcattcc
    23341 aactattcat cagcgtgaaa atccctaatg ataaaagagt aggtttgctc aatggcaaca
    23401 tgattttcaa aaaaatatta aactcactta atccatcaat gcgtgctgcc tcttccaaag
    23461 aatctggtaa agtaataaaa aaggaacgca tcatgaagat accaaatgca gttgtcatac
    23521 gaggaatgat caaacctgca taagtatcaa gcatgccaag caaattgact tcaataaata
    23581 gaggaatcat gaagacttgg aatgggatca tcattgtcag caaaacgaaa tagaacaaaa
    23641 ctgattttcc tttgaaacgc atacgagcaa atgcataccc cgcaagagaa tcaaagaata
    23701 cagaagtgat cactgtccct ccagcaaaaa tcactgtatt tttgatgtaa tctaataaag
    23761 ggatcgttgt ccaaacctta atatagttat ctagggtata gctatctgca aatattctag
    23821 gcggaaatga gataatgtct ttttcgtgtt taaaagaaga aataatcagc cacaaaaaag
    23881 gaaacgcaat aaccaacaat ataactagca tactaatcgt tcgaatgatt tttttaccac
    23941 taactgcttt tcgactattc acagtaactg taatcgagct ttctggtgcc atacaggttt
    24001 ccctcctatt tcatttgttt ttctttccgt gacatgtaca cattagtgga gatagctata
    24061 gttgcaacaa tgataaacaa aaccgaagac agtgctgatg catagcccaa ttcataaggt
    24121 gctgtaaatc ctcgactgta aatatattga acagctgttt ctgtcttaaa ttgtgggcca
    24181 ccagcggttg caacatatac ttggtcaaac acttgcatcg aaccaatcag attcgttaaa
    24241 atacaaaaac ctaaggaggg aacgatctga ggtagcgtaa tgttaaaaaa ctgctgtcga
    24301 tttgttgcac catccatttg agcagattca tacaaagagg gagagattcc ctgaatagca
    24361 gtcaagagaa tcgtcatagt aacaccaaaa ttcttccaaa cagtcatgat ggctaccgtc
    24421 ggcatagcaa gggttggatc gcgaaaaaat tgagggatcg gcaagccgac ttgtgaaaac
    24481 aagtaaggaa tcgcccctac attcggatca agcaacatag aaaaaataat cgcaattgac
    24541 gtcaatgaac aaataactgg aatgtaaaat gtcgaacgac aaaaacgatt gaatctggta
    24601 tttttagata atgctactgc tgctaataaa ccaacgataa tttgtgttgg cgtttcgagt
    24661 acggtgaaat ataaagaatg aatcaacgaa ttaactgctc gttcatcact aaatatacgt
    24721 gtgaaatttt caaaacttac aaaatttctt gatgtaaaga aaatattcaa atctgtaaaa
    24781 ctaatagcaa atgttcctaa aagcggtatg acagtaaata ataataaaac taaaaaagat
    24841 ggagttaaaa acaaatacgc agcacgatca ggacgattcc aatatttggt gaatccgttc
    24901 atatagtcca cctcatcttt cattttgata aaatatacaa tcttggttta aacgaatctg
    24961 aataagatat ccgaacaatc gctgtgattt ttgcaataac acctatctgc aaatactagg
    25021 tcgatcattc agatatctgt atcttgccaa cagtctatcc tgtataaagt tctgtactat
    25081 ttttgcagta attgattgat ggcatcatct gcatcgttca tgagtgtctc aggatcttct
    25141 cctgctaata atttttcaat cagtggattg atgatatcgt tattgatcgc agagatttgg
    25201 gtaaaacctg gtaaatatgg ctctgcatag tccatttgtt tcgataattc agatacaata
    25261 ggatcatttt ttacttcttc atcttttgct actgaatcca agtaggcagg aaaaccattt
    25321 tcgatactcc attttttacc aatttcagtt gtattccaat atttcaaaaa ctcatagaca
    25381 gcttcttttt tattattatc tgtacttgcc ggaatggcat atcctacgcc atctaaaata
    25441 gctgtttttt tgtctgaatc actcccttgt ggtaaggtag tcacattata atcaatctcg
    25501 tttgctttca atccgttatt taaccatggc ccattgattt caatggctag ttgaccagca
    25561 ttcattaaat tgtccatttc ggcgccagaa atcgattctg gaccagtctt gtctacatgg
    25621 atcatttttt ggatcgcttt taacgtttta aggttctcag gagacgcaaa ttcagactta
    25681 gtataatcat cgttcactaa tttaccgcca tttgctaaca tccagttata taacggtgca
    25741 gttccatcct tattgaagac aaaacctgat acatttttac tactatcagt taattttggt
    25801 gccatttctg tcaattcttc ccaagtagta ggtggttgtt cagtatctaa tccagcttgt
    25861 ttaaacaaat cattattcca atacatatac atgccttgta cctgcatggg gacaaaatac
    25921 tgtttacctt ctacttcacc taattcaata gccgtttctt taaaatcaga tttatctact
    25981 ccatcatatt cccagaaatc gtctaatggt tgtaccgctc cgttttctac atactgtgca
    26041 aaatctggat aatttaatgc aataaagtca ggtgcttttt tagaagaaat cgctgtagga
    26101 agtttttcat tcaaattggc ccaagtcatg atatccattt caattttgat gtttttatca
    26161 tttgttttat taaattcatt aacgatttct tgcaatattt caccatcaga tgcagtaaac
    26221 ccattccaaa aagtcaaagt cacttcttta tcagaatcag attctgctga acttccgcca
    26281 ccgcaagctc ctaaaatacc tgttgcagct accaacaaag agccaattaa ccatttttta
    26341 cctttcattt tttgttcctc cttcatctga taaacgacat ctgaccacat tccaagatag
    26401 cggtttcaat aaacaattga ttttcccatc ttcagctttt acatttgttg aatgacttgg
    26461 ttttacttgt tcacttttag gaagattagt tgcttttgta tcaaatccac tcatttcaga
    26521 aaaatcaatc actccctcta atgataattc agacaagtga acagttagat tcatttcttc
    26581 ttctccacgg ttaactgcaa atatgacaag ttctttgtca tttattacgg ctatactttc
    26641 taaatacggc acttcttcaa aatcttccgt cgcatagttt gctgaacgaa tttgtgggct
    26701 taacacctct ccttgtccat acacagcagc ttgcataaat ggataataga tcgtctgtct
    26761 ccaagcttct ccgtttgttt ctgtcctgat tggtgcaata acatttacca attgagcaag
    26821 acaagcaatc tttactctat ctgcatgttt caataaagtg attaatagac agcctaatag
    26881 taatgcatct tcaaaattat agttatcttc tagtaaagga ggagcaatct gccaaggcgc
    26941 tgcttgctca tcttgttccg ctgaatgata ccaaatattc cattcatcaa aggacaggtt
    27001 gatctgcttc ttactatgtt tcttcgcctt gactgtatcg cacatcgctg tcacttcaga
    27061 aatgaaccga tccatgtcta aggaacgtgc aagataatga gaaagatcat ttttttggtt
    27121 gccataatat tgatgcagag acaaataatc tatttgatcg tacgcttgat caagaactgt
    27181 taactcccaa tcaccaaatg taggcatttg ccgattcgaa cttccgcata aaaccacctc
    27241 aattgaatca tctacgagtt tcatcgcctt agccgtttcc tctgctaacc gtccatattc
    27301 ttccgctgtt ttatgtccaa tctgccaagg accgtccatt tcattcccta aacaccatgt
    27361 cttaattcca aaagggctct cttgaccatt cgaacgcctt aaatcactcc aataagttcc
    27421 tttaggaaaa ttacaatatt cgactaaatt acgagctgca tctactccac gagtacccag
    27481 attgacagcc atattgattt cggcccccac atgctgacac cagttataaa attcatgaat
    27541 cccaacttca ttcgtttcaa ttgtccgcca agctaaatcc aaccttctag gtctatttgc
    27601 tatgggccca atactgtctt cccaacgaaa tcctgacaca aaatttccac caggatatct
    27661 aatcatggga atgttcaatt cttttaccaa gtgaatgacg tcttggcgaa atccactttt
    27721 attagctgat ggatgatctg gctgataaat cccttcatat actgctcgtc ccatatgttc
    27781 aataaatgaa ctgtacaacc gcggatcaac tttgcctaca cgcttgacac tactaatcaa
    27841 cattgtggct tccataactg acctccttta atttatacat gaataataca cctcgttgaa
    27901 agcgttgtca ataatataaa tgttaaactt acttattttt tatcgatttt aattaaccaa
    27961 aatattaata atgattaaaa caaataattt aatataactt ttattaacaa atatgttaat
    28021 atataagaaa aaccttttaa gaggtgatat atatgcaact acaaataaac gaagaatcct
    28081 taccggtata tgaagcatta gctagtaaaa caaggatcaa gatcatccag ctactttcca
    28141 aaaaaaagat gaatgtcaaa gatttggcaa aagaacttgg agtgagcagt gctatcacga
    28201 cgatgcacgt gaaaaagcta gaggaagcaa acatcatcaa aacagaaaaa gtaggacaac
    28261 aaaaaatttc gagccttcgt gtcgataaaa tcgatatcag ttttccagaa aaaatattca
    28321 atgcttttga tacgaaagaa acatcgatac ctattggtca ctatacaaat tatgcaattg
    28381 aaccaacatg cggtttagct acgatccatg attttatcgg gaaagtggat gaaccacgct
    28441 attttatgga tcctcgaagg atggatgccc gcattctctg gtttaccagc ggatttgtcg
    28501 agtatcaggc acctaatttt ttaaatacag aggatacatt ggagatgttg gaagtttctg
    28561 tggaaatttc ttctgaattt cctttttcaa atgacaattg gccttcagat atcactttca
    28621 gcctaaatgg tgtggagcta ggaacatgga cgagccctgg agattttgct gatatccgcg
    28681 gtaaatatac gcctgattgg tatccagata atcttaatca atacggcttg ctgaagacga
    28741 tccgaattac caaacacttg acgaatatga atggagaacc cttgtcaaac atcacgatca
    28801 acgacatccc taaagagcaa gacacttggc atttgcgaat cgaagtgaaa gacgatgcca
    28861 aacatgtggg gggctgtact ttatttggta aaggattcgg taattacgat caggatatca
    28921 aattaaaagt ttactacagc tgatattttt cttttctatt ttataaaaca aaaacagcca
    28981 atccctatga agggacggct gtttttgctt tattgttatt tccagaaatc atccaaaacg
    29041 gaaattggca aatgtcgttt atgttgtgtc ttgttccacc atgcttcgat tttttcttgt
    29101 gcttgagcgg gaacttcttt tccttctaga taatcatcaa tttcattata tgtgacacct
    29161 agtgcgactt catctgcaat cattggtttc ccatcttcaa gatctgcagt cggaattttt
    29221 gtgtataatt tttccggtgc attcaatgct tgaagcaatt gtttcccttg tcgtttgtcc
    29281 aaacggaata atggcaaaat gtcggctcca ccatcaccaa atttcgtgaa gaatccagta
    29341 atgttttctg cagcatgatc tgtccctaat acggctcctg ctctttctcc agctacggca
    29401 tattgcgtaa tcatacgttg acgtgctttg atatttcctt tgttaaaatc gctgattccc
    29461 acacctgctt cgcttaatac ttgtacttgt gcatcaacag ctggtttgat atttactcgc
    29521 agactaacat cgggctggat aaagtcaaga gcagcttttg catcttcttc atctgcttgt
    29581 tcaccataag gaagacgaac agcgataaat tgatagtttt catcttttgt ttcttcacgc
    29641 atttcttcca tcgttagttg cgccaaacgg cctgctaaag tagaatcttg gccgccgctg
    29701 atacctagta caaaagtttt aagaaacgga tattttttca aatatgcttt cataaagtca
    29761 atacttttac gtatttcttc ttttggatca attgttggtt tgacacctaa ttcattgata
    29821 atttctgtct gcaggctcat ttattctcct cctatgcttc tgtatgaagt tctgctacgc
    29881 tttttttcat tcgttccatg atagccattt tatggttcca gcattcagta gacagatcga
    29941 ctgggtattt ttgcggattc aagtctcgtt tatactcttc ccatagtgaa tctagatttt
    30001 cttttgtata ttctttgatt tcgtttagtg caggtaattc gtaaactctt tttccttcga
    30061 taaagatatc ttgtaaaact gggcgagctg taaagtctcg aacagtttta ttgataaacg
    30121 tatgcactgg atggaacatg aagatagctt cttcttctcg agggtcttca tcccataatg
    30181 tcacgtagtc tccttcagat ttcccatcaa aatttcgagt gatgcgccac acttgttttt
    30241 tccctggagt agttactttt tctgcattgc ttgataactt gatcgtatcc atcatttttc
    30301 cttcatcatc ttcgatcgat actagcttga atactgcacc aagggctggc tgatcatagg
    30361 ctgtgatcaa ttttgtccca acaccccata catcaatttt agctttttgc atctttaaat
    30421 tcaaaatcgt tgcttcatcc aagtcattcg aagcatagat ttttgcttcc gtaaatccgg
    30481 ctgcatctaa ttgttcccga acacgcttag atatataggc catatctcca ctgtcgatgc
    30541 gtacgccttg gaagttgatt ttgtcaccta attctttcgc cacgcgaatc gcacttggca
    30601 cacctgattt caacgtatca taagtgtcga ctaagaacac gcagtcttta tgggttttag
    30661 cgtaggccat aaatgcttca taatcatttc cataagattg gactaacgaa tgagcatggg
    30721 tcccgctagc tggtatccca aatatcttcc cagcacgtac attgcttgtt gcatctgctc
    30781 cgccgatata tgccgctcta gttccccaga ctgctgcatc cagttcttgt gcccgtctag
    30841 ttccaaattc caatagtgga tcttctccga tgacagattt gatacgagct gcttttgtcg
    30901 cgatcaacgt ttggaaattg accatattca acagtgctgt ttctactaat tggcagtgcg
    30961 ctaaaggccc ttccacttgg attaatggtt catttgcaaa aactagttct ccttcacgag
    31021 cagaacgaac tgttgcttta aattcaaaat tttgtaaata ctccaaaaaa ccttcaggat
    31081 agacttctaa tccacgcaag taagcaatat cggaatcatt gaacgtcaga ttctctaaat
    31141 aattgactaa ccgttccaat cctgcaaaaa ccgcgtatcc gtggttaaaa ggcatttctc
    31201 gaaagtaaca ttcaaaaaca gcatgtaaat cagctcgacc taattcccag tatgtctgca
    31261 tcatattgat ttgatataga tccgtatgca gcgtcaaact gtcgtctggg taagtattat
    31321 tcatcttgta ttactcctct tggcaag
//
LOCUS       DASJTC010000023        30852 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.023, whole genome shotgun sequence.
ACCESSION   DASJTC010000023 DASJTC010000000
VERSION     DASJTC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.023"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            15..164
                     /locus_tag="U4B19_000956"
     CDS             15..164
                     /locus_tag="U4B19_000956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076714.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            complement(198..362)
                     /locus_tag="U4B19_000957"
     CDS             complement(198..362)
                     /locus_tag="U4B19_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076715.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            657..1985
                     /locus_tag="U4B19_000958"
     CDS             657..1985
                     /locus_tag="U4B19_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEN2076716.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2187..3020
                     /locus_tag="U4B19_000959"
     CDS             2187..3020
                     /locus_tag="U4B19_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEN2076717.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3036..3773
                     /locus_tag="U4B19_000960"
     CDS             3036..3773
                     /locus_tag="U4B19_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEN2076718.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3928..4896)
                     /locus_tag="U4B19_000961"
     CDS             complement(3928..4896)
                     /locus_tag="U4B19_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEN2076719.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5128..5961
                     /locus_tag="U4B19_000962"
     CDS             5128..5961
                     /locus_tag="U4B19_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEN2076720.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5995..6834
                     /locus_tag="U4B19_000963"
     CDS             5995..6834
                     /locus_tag="U4B19_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2076721.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6860..7420
                     /locus_tag="U4B19_000964"
     CDS             6860..7420
                     /locus_tag="U4B19_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN2076722.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7619..9340
                     /locus_tag="U4B19_000965"
     CDS             7619..9340
                     /locus_tag="U4B19_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEN2076723.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9505..10647
                     /locus_tag="U4B19_000966"
     CDS             9505..10647
                     /locus_tag="U4B19_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2076724.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10663..11874
                     /gene="thiI"
                     /locus_tag="U4B19_000967"
     CDS             10663..11874
                     /gene="thiI"
                     /locus_tag="U4B19_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEN2076725.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12447..13094
                     /locus_tag="U4B19_000968"
     CDS             12447..13094
                     /locus_tag="U4B19_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEN2076726.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKLLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13223..13439
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13487..16132
                     /locus_tag="U4B19_000969"
     CDS             13487..16132
                     /locus_tag="U4B19_000969"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEN2076727.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16442..17755
                     /locus_tag="U4B19_000970"
     CDS             16442..17755
                     /locus_tag="U4B19_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEN2076728.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17745..18398
                     /locus_tag="U4B19_000971"
     CDS             17745..18398
                     /locus_tag="U4B19_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2076729.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18453..19145
                     /gene="radC"
                     /locus_tag="U4B19_000972"
     CDS             18453..19145
                     /gene="radC"
                     /locus_tag="U4B19_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEN2076730.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19295..19495
                     /locus_tag="U4B19_000973"
     CDS             19295..19495
                     /locus_tag="U4B19_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2076731.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(19749..21428)
                     /locus_tag="U4B19_000974"
     CDS             complement(19749..21428)
                     /locus_tag="U4B19_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN2076732.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(21430..21642)
                     /locus_tag="U4B19_000975"
     CDS             complement(21430..21642)
                     /locus_tag="U4B19_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEN2076733.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            22035..23765
                     /gene="efrA"
                     /locus_tag="U4B19_000976"
     CDS             22035..23765
                     /gene="efrA"
                     /locus_tag="U4B19_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEN2076734.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            23762..25522
                     /gene="efrB"
                     /locus_tag="U4B19_000977"
     CDS             23762..25522
                     /gene="efrB"
                     /locus_tag="U4B19_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEN2076735.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            25617..25814
                     /locus_tag="U4B19_000978"
     CDS             25617..25814
                     /locus_tag="U4B19_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076736.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            25967..26452
                     /locus_tag="U4B19_000979"
     CDS             25967..26452
                     /locus_tag="U4B19_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEN2076737.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(26556..27149)
                     /gene="clpP"
                     /locus_tag="U4B19_000980"
     CDS             complement(26556..27149)
                     /gene="clpP"
                     /locus_tag="U4B19_000980"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEN2076738.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            27329..27856
                     /locus_tag="U4B19_000981"
     CDS             27329..27856
                     /locus_tag="U4B19_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076739.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            27947..28684
                     /locus_tag="U4B19_000982"
     CDS             27947..28684
                     /locus_tag="U4B19_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN2076740.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            28688..29605
                     /gene="miaA"
                     /locus_tag="U4B19_000983"
     CDS             28688..29605
                     /gene="miaA"
                     /locus_tag="U4B19_000983"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEN2076741.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            29607..30836
                     /gene="hflX"
                     /locus_tag="U4B19_000984"
     CDS             29607..30836
                     /gene="hflX"
                     /locus_tag="U4B19_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEN2076742.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10183 a         5277 c         6587 g         8805 t
ORIGIN      
        1 gttggtagta gcgcatgact gttaatcatg atgtcgtagg ttcgagtcct actgccggag
       61 taaataaagc ttatgcaaag aaaaagaaat ccaatcactt gggtttcttt ttcttttatt
      121 attatgatga atcaagcttt ctgtttttgt cacgtatgtt gtaatcttta cgaaagagta
      181 gagttggcta ctcataatca accatacttc ccatcatcgc taaatacgaa ataaatcagc
      241 attcctacac caatcaaagc acaagctgca ccaagaaaag ggatgacatt cccaaagtta
      301 tctgtattca taagtatgcc accacccaca aacaaaaagc cgccagcaat caggtttttc
      361 attttattca tctcctttct tacgttatag tatttccttc tttcaatgag tattttgaaa
      421 gctatcttat tgtatgatgt tcttctaggc ctcttcttta ttatacaggc cattttcgtt
      481 atctcataga aatattcctt tttatctgaa aactattgta taccgtatga catttttctt
      541 catgaagttg taagtatatt ctcattcaaa tgaaaatttc atcatagaag agattgtatt
      601 tctaaattct taagagtaaa atacgaagta taacttggat ttggaaggat gattggatga
      661 aagctattac gcctgatatg acaacaaaat ttaacaaaca gtttcaagca aatatcaaac
      721 agcaggcatt gcagcgggca gttgtgaaga acggaattac tgcttctgct gaaaatgttt
      781 cagcacatgt ggagaataca ccggtatttt ctatagattt ggctacgggg aaagtggcta
      841 atcaaatgca atcggggcgt tgctggatgt ttgctgcact gaacacattc cgtcataaga
      901 tgctgaatca attccattta aaagagtttg aattatcaca aaattacact tttttctggg
      961 ataaatatga aaaggcaaat tatttctatg aaaacatcat tgcaacagga aaagaggcaa
     1021 tcgatagccg gaaagtagct ttcttactcg ctactcctca acaagatggc ggccaatggg
     1081 atatgatcgt ttcattattc caaaaatatg gcgttgtgcc taaatcggtg atgccggaaa
     1141 gtgcgaatag ttcaaattct cgagatttga ataactactt gaacaaatta ctgcgtaaag
     1201 atgcagtagt gttacggaaa atggttgctc aaggagaaac attagacgct attgaagaaa
     1261 agaaagaaga aatgctagaa agcatttata acttcttagc tatctcatta ggaacacctc
     1321 caaaagaatt cgattttgag taccgcgatg aagaaaagaa ctatcatctt gaccgaggac
     1381 tgacaccaca aattttttat gataaataca tcggtgtaaa attagatgat tatgttagcg
     1441 tgatcaatgc accaacaaaa gacaaaccat tcaatcgttc ttatacagta gagatgctag
     1501 gaaatgtcgt tggcgggaaa gaagtcaaat atctgaatgt tgatatggaa actttcaaaa
     1561 aattagcgat tgctcagctt gaagaaggag aatctgtttg gtttggttgt gatgtaggtc
     1621 aatcttctac tagaactagt ggcatcatgg ccttagatgc ttattcaatg gatgatctat
     1681 ttgatacgga ttttacaatg actaaagctg aacgtttaga ttttggagaa agtctgatga
     1741 cccatgcgat ggtattgacc ggtgttgact tagtagacgg gcaatcaaca aaatggaaag
     1801 tcgaaaatag ctggggcgaa aaagttggaa agaatggctt ctttgttatg agtgatgcct
     1861 ggatggacga atatacgtac caaatcgttg taagaaaaga tttattgacg aaagagcaat
     1921 taaatgcgtt caatgaagaa ccaattgttc tatctccgtg ggatccaatg ggtgctttag
     1981 catagacgag tatttcaatg aagagtatgt agtaaagata aaaagagctg gcttaagcca
     2041 gctcttttta tctttaaagt ccattccctg ttttttatgg tccctgtgct ataattctaa
     2101 ataaaggata aggattggaa ataatccttt tatcattaaa aatatgatga cgaagtagaa
     2161 taaaattggg agtagggggg aaagtcatgg atatccaaac tgttttatct gatctgaaat
     2221 taaatggtaa agtgattcca gttgttggtg gcgatgttaa tcagacctat cgtataaaaa
     2281 ccgaacatcg tgcttatttt ttaaaaatcc atccgaatgt aaaaaaagga ttttttgaag
     2341 cagaagtaga cggattaaaa gaactgtcag cattcgtacg tgtaccggat acgtatatgc
     2401 taggtgaaac aagtgaaggc gcttatttgt taatggaatg gatcgagccg ggaaaagggg
     2461 atcaacgaga tcttgctgca gctttagcta atctccatca gcaaacagcg ccacaatttg
     2521 gttttcgtaa agataactat ttgggtacac ttgttcagaa aaattctttt gaagaagatt
     2581 ggtggacttt tttctttaaa gatcggcttg aatcccagat ttctttggca gaagaaacga
     2641 atcgatggaa tgtacagcgt caagaaaagt atctgcgatt taaagaacgt gtattgaaat
     2701 cggttgaacc aaaaaagatc actccacgcc tcttgcacgg agacctgtgg agtggtaatg
     2761 tgttctttga ccagcaaggg catccagttt ttgtcgaccc agctgtttct tatgggaatc
     2821 gggaacaaga tattgcaatg agccagttat tcggtggttt ccgaccggaa tttttagatg
     2881 cttatcaaac tatatttcca ttagaaaagg gatggaaaga ccgactgccg atttaccagt
     2941 tatattatct tttggcacat ttgaatatgt ttggagaaag ttacggttcc caagtagatc
     3001 aattactaga aaatttttaa gaggagggcg tgtttttggc aaaaaaatat accaaaaagc
     3061 atgaagtttc atattatgag tgtgacatca accaaacgat gacatttcct tcgatgttag
     3121 gagtagttat caaaacgtct gaagatcaaa gcgaccagtt gggaagaggc agtgaattcg
     3181 tcaatagctt tggacttact tgggtcatca cgaattattc catggagatt aatcgtctcc
     3241 ctagagtcgg tgaagtaatc tcagtaacga ctcaagccat ggagtacaat aagtttttct
     3301 gttatcggaa cttctggatc catgatgaag atgggaaaga attagtcaaa atagaatctg
     3361 ttttcgtatt gatggatttt gtgaatcgaa aaatgagcag tgttaatgaa gagatcattg
     3421 ctccttttga gagtgagaag atcaagaaaa tcaagcgtca ggaaaaaatt gaaaagattg
     3481 attcgggctt catgttgcca tatcgtgttc gtttttatga tatcgatagt aatcagcatg
     3541 ttaataatgc gatgtatttt aattggatca ttgacgtatt aggatatgac tttttgacta
     3601 cgcatgttcc agaaaaagta attattcgat ttgataaaga agtagagtac gggaatgaga
     3661 tcgaaagcca ttttgaacaa atccctatcg aaaatggtgt caaaacaaaa catgaaatcc
     3721 gaatgcaaga acagctgtat tgtgaagcaa atattatatg gaaaaataaa taaaaaagag
     3781 gttgtgacag aagggggcaa cttcaagaaa taagaagaaa tttacgaaaa ttgtttctca
     3841 aatttttgta aattttcgac ttagttttcg aagaagctat ttttgtcaca gcctctcttt
     3901 ttaagtttag ctattagata atgatgttta gttttggata aacgaaatca attcttcacc
     3961 cgacaaacca tagtttacag caatcaatag ttttaaacgt gctttttgac tattaatact
     4021 gttacagaaa ataatcccca ttttttctag ttcttttccc ccgccttcat aatcataaac
     4081 aggctctgcg attccattga aacaacgaga aacaagtact acagggattt tttggacaag
     4141 aagcttttcc aaagcagcta aggcttgtgg agggagattc cccgcaccta aagcttcgat
     4201 taccaaaccg tctactttcg tatgggcgat ggcttccaat aagtcccctt gcatcccagc
     4261 atatgcttta acgatcggga tagtcccatc tacagcggaa atgtccagtc gctttgtttc
     4321 taaaagttcc tgcaagaata aaattcgatt cttagttact aagccgatgg gacccaatgt
     4381 aggtgttcgg aaagtagcaa catttgtggt atgggtcttt gtcacgtagc gtgcagaatg
     4441 gatctcatcg ttcatgacca ccaaaacccc cttatctagc gcttcttcac aacttgcgac
     4501 acgaatcgca ctttcaaaat tataaaggcc gtcacttcca agttcattac ttgaacgcat
     4561 tgccccagtc aaaacaattg gtaatttgtt gccaatcgtt gtatcaagaa aaaaagcagt
     4621 ttcttctaat gtatctgtac cgtgtgtgat caccactccg gatatacctg atgcataagc
     4681 ggacaagata cgttctttta ataccaacat gtgagaaggc atgatgtgtg gtgagggaag
     4741 attgaagata tcttcaacta ataattctac atgcttgggg attgtgattt ctgcatctaa
     4801 taatgggttt gccacatcag gactaacacc gcccgtgagc atgttttctt tcatggcgat
     4861 cgttccgccg gtgtgtagta caagtatttt tttcatttta cagacttcct tacgaataat
     4921 tttgcatagc cgttaaatct tattgtatac tatataaaag agttttgcta tcagtgaaga
     4981 ataacgaata gcctaactta tcataaaaaa gctgacctta ttaaaatgga aaagactcaa
     5041 ccttgacctc ctaatacaat ggagtatctg gttgagtttc ttttacaaga aagcagccaa
     5101 ggaaatcaat cacgaaggtg gccggaaatg atcagaatcg gattaacatc gtttaaagaa
     5161 catgacaaat taactggtaa aaagaataac actctttatg aatatgcagg gtttctaccc
     5221 ttagtggaat tagatactgc ctattatgga attccccgaa agtcctcagt tgagaattgg
     5281 ataagtgaag tacctgaatc atttcgcttt gtagtcaaag tctatcaagg aatcagcggt
     5341 caaggagact ggaagcctta ttatcagacc gaagaagaga tgattgcgca ttttttagat
     5401 gttatgtcgc caatggtaga tgctggaaag atgttttgct ttttagtcca attcccctca
     5461 tcattcaaat gtacgaaaga aaatgtccag tacttgcgca aacttagaaa gtggtttgag
     5521 ggttatcctg tttctataga attgcgggat tattcttggt acagcgatga atttcttgaa
     5581 cgtacaagaa actttatgtc tagagccgct ttttctttag ccatcatcga tgagccacag
     5641 ttgctgaata caacgattcc ttttgatcca tttgtcacca atccgaactt tgctcttttt
     5701 cgattccatg gtaggaatgc gcaaggatgg caagcaaatg ataaagagtg gcgtaaaaaa
     5761 agaacgttat atcgttattc agaaaaagaa ctgcaagaac tagcacatga agtcaaagta
     5821 gttgattcta agacaaaaga ggtaggtgtg atttttaata ataattctgg tggtgatgcg
     5881 gctgaaaatg cattagcact gcaaaaaata ttaaatatca agccagatgg actcaatcca
     5941 acccaaatgg atctgtttta agggcttatt ttattcaaga aaggggcgaa gtctatgatc
     6001 aaagctattt tttttgatat tgacggaaca ttggtcacca atcgttcaaa agcgttggaa
     6061 tcaacaaaac aagcaattga atatgctcgg aaaaacggca ttttatgcgg tgtagcaacg
     6121 gggcgcagcc cagtaaagat caaagaaatc attgatgaat tggaattaga tatgtatgtc
     6181 gtgtataatg gtcaactggt ttttactgca gatcgcacga ttatcgacca cccttttgaa
     6241 caaaaagtat tggaacatat tgttgaattt gcagatgaga accatcgaca aatcgttttt
     6301 ggtgcgagaa atcggttaga tggatcaacg acgatgctat taggacaatc gatttttatc
     6361 aagcgactag tcagcttttt accgcgtaaa ttcccggtac gtctgatgaa aaaaatcctc
     6421 caggttttta gccctcaccg acaaaaggat cgttatgaaa cgttagagat attaaaagaa
     6481 ccaatttatc aatgtatatt gctcagtccg gagtcagaac agaaaaagtt agaacatcgc
     6541 tttcccgagt gtacgtttca gcgttcaaat acgtatactg tagatatcat cccaaaaggt
     6601 ggttcaaaat tactaggcat ccaagctttc gcaaatgctg tcgaaattga aatggaagaa
     6661 attatggcat tcggcgatca ttacaatgat atcgagatgc tgaaaggtgt aggaattgga
     6721 gtggccatgg ggaatgctca gatcgaagta aaacatgctg cagactttgt gactcaatca
     6781 aatgaagaag atgggatata cgaaggactg aaacacttcg gtctgattta ttagtcaatg
     6841 aaagaaggaa aaaatcagta tggaagcaga gacacctttt caaaaagtag aaacattcca
     6901 caaaactttt gatccaagaa gaccagagat acccacggct tttacagaaa atgaagctca
     6961 tacacgtgct gcgttcaaaa tagaagaact tgttgaattt gttgcagcta cttcacctaa
     7021 taaagaacaa ctgcatgaag ctgttatgct gctgcacgaa gcattagata aagcagaaga
     7081 aaaaatactt ggtaaaacac agtggggaag tgatccgctg gttgaacaag tagatgcact
     7141 gacagatatc ctttacttta cttatggttc tttttcatta ctaggaatag tgcctgatcg
     7201 aatattcgaa atcgtccatc aggcaaatat ggggaaatta tttccagatg gaaaacctca
     7261 ttatgaccct gtaacaaata aagtgttgaa acctgaaaac tggcagatgg aatatgctcc
     7321 agaacctaaa atcgcggcag aaatccaacg tcagattcaa gaagcgattg ctaagaagaa
     7381 atccgctgat ataggcatgt gcacaaatga aaaagagtga tcagagtttc actatttttg
     7441 ttatctttat acgatggaaa aacaaaagat tttcgtttaa gttagtgaat gcatgaaaat
     7501 agtatgctat aatctagaat agatgtcttt tgcacgagag acgtattatt tggatttatg
     7561 ccaagcagaa cgccatctgt tgcataaaag atgcatactg aaaacggagg aaccataaat
     7621 gggaaataat gttatcatcg gaatcgtcat tgcaatcatt atagtcgcca ttgtcgtctt
     7681 tttggttagt tacttgatga agaaaaaaaa ccaagatcga ttggatgcct tagaaaaacg
     7741 caaggaagaa ttatttgact tgccagtgat tgaagaagtc gatgatgtta aaaaaatgca
     7801 tctggttggg caaagccaaa atacctttcg tgaatggagt caaaaatgga atgacatatc
     7861 tacttcctcg ttcgctgaac tagaaagtca aattttcgaa gtggaaaacc tcaatgaaag
     7921 ttttcggttc ttaaaaggaa aaaaagcagt tgttgaagca gaagctacaa tggaccaaat
     7981 ggagaaacaa gtgacggaaa tccgtgaagg tctaaaggaa ttgcgggaaa cggaagaacg
     8041 caactcacta gctgttcagc aggcacttga cgtctatgaa gaactgaaag aaaaagtcag
     8101 agaagacgag catgaatacg gaccagctct ttttgaattg caaaaacaga tcaaagtgat
     8161 cgaaaaagaa tttacgcagt ttgtgacttt aaacacttct ggagaccctg ttgaagcccg
     8221 cgaggttttg gataaagcag aaaaacatac gtatgaagtg gaagctctga tgaaaaaaat
     8281 tccaccgctg tatgaggagc tccataccac tttccctgaa caacttgaag aaatcagtga
     8341 tacttacgag aaattgatgg aagaacaata tgtattccca gaagaagatc ttgctgaaga
     8401 tatcgcaaaa gtaagtcgaa gaattgaaaa ttcattagcc aatcttgaaa aaacagaagt
     8461 tgaaacggtc gaatttgaaa accgtgaaac agctgatttg attgattcgt tatatgatat
     8521 cttagaacgg gaaatggaag ctcagagata tgtaaaaact aaccaatcaa cgattgcaga
     8581 gtatatcaaa catacaacaa agaataaccg tcaattattg attgagctgg atcacacatc
     8641 tcaaagctat acattgaacc acaatgaaat tggccgtgta cgaggtttcc aaaccgaagt
     8701 ggaagaaatg gaacgtcaaa atgaacagat gattccacag atccgtcaac acgaaattcc
     8761 ttattcagaa gtgcgcacgt tctataaaac agtcttcaaa gtcttagaag atatcgaaaa
     8821 gcaacaagta gagatcgacg actcattaca tgaattgaga aaaggcgaaa aagaagcgca
     8881 agaaaagatc gatacattcg aattcaaatt gcgcagtttg aaacgttttg tagaaaaaca
     8941 acgtttgcct ggcttgccga atgattacct agaattcttt tttgttgcta ctgaccgtat
     9001 tgaagaacta agtgttgtct tgaataaaat tcgagtaaac atggaagagg tcaatcgttt
     9061 agttgctcta tgtgaagaag atctagaatt attagataag aagacacatg atttagtaga
     9121 tgcagcagca ttaacggagc aaatgctcca atatgcaaat cgttatcgtc atacacacga
     9181 agaagtgaga gaagcaattg ataaaagcta ctatttattc aataaagaat atcactatca
     9241 agaagcatta gacgaaatcg gtacagcatt agagcgagta gaacctggtg ctttcaaacg
     9301 catcgaagat ttttatttta atcatccaga cttagtataa agcaaaaaag ttagtaaacc
     9361 agcaagagca agacggatta ttgtcttgtt cttgtctttt tttgtatatt tgaaaattga
     9421 aacaagtgaa aatttcacct gctcttgaaa gcaccttgga caaccgttat aatagaaaag
     9481 aatttcaaaa aagaggggtt tcttatgata tattttgata acagtgcaac aaccccaatc
     9541 tttccgcaaa gcttagatgc atatgtaaaa accagtcagc ggattattgg gaatccatca
     9601 agcttgcatg atttaggcaa tcaagctaac cggttgttgc agcaagcaag aaagcagatc
     9661 gctgatttga tccatgtaga accagaagaa atctatttta cttcaggcgg aacagaagga
     9721 gataactggg tattaaaagg aacgatgatc gaaaaaagag aatatgggaa tcatatgatc
     9781 atttcaggag tcgaacatcc ggccgtcagc gagacagcag aacaattaaa agaattagga
     9841 atcgaagtaa gtatcgcccc agtggataaa cgcggatttg tacgtgtcga cgaattgaaa
     9901 gaattaataa gaaaagagac agtattagtc tctgttatgg ctgtgaataa tgaagtagga
     9961 tcgattcagc ccatccagga aatcagtgat cttttggatg attttcctaa aattcatttc
    10021 catgtagatg ctgttcaggc gattggaaaa gtaccattct cgcaatggtt gacagaaaga
    10081 gttgattttg caacattttc agctcataaa ttccatggcc cacggggaac tggtttcatt
    10141 tattggaaaa agggtcgtcg tttagcacca ttactgacag gtggagggca agaaagaaat
    10201 cagcgaagtg ggacagaaaa tgttccaggt attgtggcta tggttaaagc tttacgttta
    10261 tcattagaga taaaggaaca aaaacctgag cagacacaac gcttaaaagt agctctaatc
    10321 aatgcgttga agacgtatga gaaagtgacg attttttcag aaggaactga ctatgcaccg
    10381 catattttat gttttgctct aaaaggaatt cgcggcgaag ttctcgtcca tgccttagaa
    10441 gaaaaacaaa tatttgtttc tactactagt gcctgctcct cacgtaaaca tatggcaagc
    10501 agtacattgc acgcaatgca tgtaccaaat gatctggcta catctgctgt tcgaatcagc
    10561 ttagatgaaa acaattcgct tgcagaagtc gagcaattta tgattatttt caagcagctt
    10621 tataagaaat tttctgcaat caattaaaag aggagtgttc ttgtgaaata cagtgaaatt
    10681 atggtccgtt atggggaact ttcgacaaaa ggaaagaatc gaaaaagctt tatcatgcaa
    10741 ctagcacaaa atgtcagaca agctttgaaa gactttccag aaatcaaaat ccatgctgat
    10801 cgggaccgga tgcatctttt attgaatggc gcagacgggc agctagttat tccaaaacta
    10861 gcaaaaatat ttgggatcca aaatttctct ccaagtattc gtgtagaaaa agatgtcgaa
    10921 gttttgaaac aagctgttca agacatcatg aaagaaattt atactgggaa agaaacattt
    10981 aaaattatcg ctaaaagaag cgatcaccaa tttgaattga caagtaatga attgaatcaa
    11041 acattaggta atgctgtttt tgatattttc ccgcatatcc aagttcaaat gcgtcaacca
    11101 gatattccat tacgtgtaga gattcggcgt gatggcgctt atctatcgta tgaaacgatc
    11161 aagggagctg gaggattgcc cgtaggaaca agcggaaaag gaatgcttat gctttctggg
    11221 gggattgatt ctcctgtagc agggtacttt gccatgaagc ggggagtgga aattgaagct
    11281 gttcattttg ctagtccgcc gtatacaagt gaacaagcac tgcaaaaagc aaaagatcta
    11341 acagccaaac tagcacctta tgtgggaacg atccatttta tcgaagtgcc atttaccgag
    11401 attcaagaag aaatcaaaaa gtcagtgcca caaggatatt ggatgacaat tactcgtcga
    11461 atgatgttgc gtttgaccga tgaaattcgc cgtattcgtc atggtttagt cattttgaat
    11521 ggtgaatcat taggtcaagt ggcttcacaa actttgcaaa gtatggtggc tatcaatgaa
    11581 gtaacgaata cgccaatcat tcggcctgta gcgacaatgg ataaacttga aatcattgaa
    11641 gtagcagaac agatcgatac atttaatctt tctatccagc cttttgaaga ttgctgtacg
    11701 atttttgctc ctccacagcc aaaaacacgc cctcaactgg aaaaggtcca tcaatatgaa
    11761 gagcgtttag caatagatga aatgatccaa cgagcactag ctggcttgaa gatggaagag
    11821 atacaagcag aatcgtctaa tgaaaaaact gaagaatttt cagaattgct ataagaggtt
    11881 atgaaaatag tgttttgacc agagtattag agaaacaaaa cgaaaaaata gctccatgta
    11941 ttttaccgtt tttgaactaa tgtcgaagat tagctgattg aataccattt atcaagaagt
    12001 tatggaaaag ccggttcttt tttactaact gaaaaataaa attttattta gagatcaagc
    12061 tacagttgat aaatgatata aaaaagagaa tttcccatga aatagcgagg ttgtgacaga
    12121 agtgggcagc ttcaagaaat aagaaggaat ttacgaaaat tgcttttcaa atttttgtga
    12181 aatttcggct tatttttcga agaagttact tctggaacac cgtgtatcag aggttgcgaa
    12241 aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagttcct catattttac
    12301 gtttggttga gcttattacc gcagatgcaa gcttttgaac accgttttat ttgtctcaca
    12361 cccttttcct tcctacttgt aaccttgctc ccgtgttaga aacatgttac actaaatatg
    12421 tgaaattatt aacaaggagc gtcgttatgc gagaaaaaac agatacactg ccaaaagcaa
    12481 ctgcgaaaag attaccttta tatttacgct acttgaagtt gctggatgac tcaggtataa
    12541 gtagaatcaa atcaaatgag ttcagcgaaa tcacccaaat tccttctgca acgatccgca
    12601 gagacttctc gcaattaggc gagctaggca gaagcggata tggctatgat gtcccttttc
    12661 ttatcgatgt attcaataat atccttaata ctaaagaaga aaaacgaatc gcattagtag
    12721 gatatggaaa tcttggcaaa gcattgaaac acaacaattt tagaagaaat gaaaatctaa
    12781 atatcgtgtg cgtctttgat aatgatccag cactgatcaa tcgagtaatt gatggagaaa
    12841 tgatctatcc gattgatcgt ttcgcggaga tagccaaagc aaaaaatgtg acagtagcaa
    12901 tctctacggt accaagtaaa tattcacaat ctgctattga tgaaattgtc aaaggaaatg
    12961 taaccgctat acttaatttt gcgcctgaca gagtaactgt cccagcttat gtcaatgttc
    13021 aatacattga tttgacaacc gagttgcaga cgcttattta ctttgatgag aattactcag
    13081 aagttttttc ttaggaaaat ttgagtaggc ttattgatct tgcgagaact cgtcgtaatt
    13141 ctttcaaaaa ttttttagta ctaaatgaag tggttttttc acgaccactt gttgactaaa
    13201 aaaaaaaacc attgtagaat gaagcataac gctgaacaag aggagtaact ggcacattca
    13261 ttcttagaga gagaactatc tgctgaaagg ttctcaatga caaccagtga aggtagcttg
    13321 ggagtttctg tcttgaatgg agtagagata gacgagtcgt gatcgttaac tcacttgaga
    13381 ggcaataatg attgattatt gcaatttagg tggtaccgcg taaatcatac gtcctagaat
    13441 acgaaagtat tttaggacgt tttttagttt aaaggaggaa aacagcatga cggaagaaaa
    13501 taacctatca acaaaatata atccgcaaga agttgaagct ggacgttatc agcattggtt
    13561 agaacaagat ttgtttaaac cgagcggaga taaaaaagca aaaccttatt caatcgtaat
    13621 cccgccccca aatgttactg gaaaacttca tttagggcat gcttgggata cgaccttgca
    13681 agatatgctg attcgtcaaa agagaatgca aggatatgat actttatggc tgcctggtat
    13741 ggaccatgca ggaatcgcga cacaagcaaa agtagaagcg aagcttagag agcaagggat
    13801 ttctcgttat gatctaggtc gtgaaaagtt catcgatcaa gtgtgggaat ggaaagaaga
    13861 gtatgcgagc cacatccgcg aacagtgggc taaaatgggc ttatctttag attacagtag
    13921 ggaacgtttt acattagatg atggattatc tgaagcagta cgtaaagttt ttgttacctt
    13981 atacgaaaaa ggtttgattt atcgtggtga atatatcatc aactgggatc ctcaagcacg
    14041 aacagctcta tctgatatcg aagttattca taaagatatc gaaggcgcct tttatcatat
    14101 gagctatgaa ctagcagatg gatcaggcgt agtagaaatt gcgacaaccc gtccagaaac
    14161 gatgttggga gatacggcga ttgctgttca tccagaagac gagcgttatc aagcgctcat
    14221 cggcaaaaaa gtgattctac ctttagtcaa taaacaaatc ccaatcattg cagatgaata
    14281 tgtagatatg gaattcggga caggtgtagt aaaaatcact ccagcacatg atccaaacga
    14341 ctttgaggta ggtaatcgtc atgatcttcc acgcgtaaat gttatggatg atgatggtac
    14401 gatgaatgaa ctagcaggaa aatatgctgg aatggaacgt tttgctgctc gtaaagcaat
    14461 cgtgcaagat ctgaaagaaa tcggtcgatt gattaaaatc gatacgatga tccacagtgt
    14521 gggtcattcc gagcggacag gtgtcgttgt tgaacctcgg ctgtctactc aatggtttgt
    14581 aaaaatggcg ccgttagcga aaaaagccat tgataaccaa gaaacagatc aagcagtgga
    14641 attcttccct ccaagattca atcaaacgtt tttacgttgg atggaaaatg tccatgattg
    14701 ggtaatctca cgacaattat ggtggggaca tcaaattcct gcatggtatc acaaagaaac
    14761 aggtgaaatg tatgtaggga tggaagcacc agcggatagt gaaaactggg tacaagatcc
    14821 tgatgtttta gacacttggt ttagttcagc tttgtggcct ttctcaacaa tgggctggcc
    14881 agatacggaa agcgaagatt ataagcgata cttccctact agcactttag taaccggtta
    14941 tgacatcatt ttcttctggg taagcagaat gattttccaa agcttggaat ttactggcga
    15001 acgtccattt gagaatgtct tgattcatgg attgattcgt gacgaacaag ggcggaagat
    15061 gagtaaatca ttaggtaatg gaatcgatcc gatggaagta atcgaaaagt atggagcaga
    15121 tgctctccgc tggttcttat cgaatggttc tgcaccagga caagatgtcc gcttcagcta
    15181 tgaaaaaatg gatgcttctt ggaactttat caacaaaatc tggaatgctt cacgtttcgt
    15241 tatcatgaat atcgaaggaa tgaccgtaga agagatcgat ttatctggag aaaaatcagt
    15301 ggctgaccgg tggatcctta ctcgtttgaa tgaaacgatc gaacgagtaa ctgagctatt
    15361 tgatcgtttt gaatttggtg aagcaggtcg acaactgtat aactttatct gggatgattt
    15421 ttgtgactgg tacatcgaaa tgagtaaaga agtactttat ggagatgatg ctgtttcaaa
    15481 acaaaccacc cgtagcatct tagtccatac acttgatcag atcctacgtt tattgcatcc
    15541 aatcatgcca ttcgttacag aagaaatatg ggaacatatt cctcatcagg gaaattctct
    15601 agtagtggca gaatatcctg ttgtacgtcc agagtttgat gatgaaacag cttctaaagg
    15661 gatggaagta ttgaaagaat tgattcgagc cgttcgaaat attcgttcag aagtgaatac
    15721 accattgtct aaaccaatta cgttgctaat taaaacaaac gatgctaaaa tcgatcaatt
    15781 tttagttgaa aatacaagct atatcgaacg cttctgtaat cccgaagagt tgacgatatc
    15841 aagtgagatc actgcgccag atctagcgat gtcagctgtt ctgacaggtg cagaaatctt
    15901 cttgccgtta gcaggattga tcaatattga agaagaaatc aaacgattag aaaaagaact
    15961 ggaaaagtgg acaagcgaag taaaacgtgt ccaaggaaaa ctttctaatg aacgttttgt
    16021 ttctaacgcg ccggaagaag tagtagaggc agagcgtgca aaagaaaaag attatttaga
    16081 gaagcaaacg gctgtcaaag aacgaatcga aagtttgcgt actattcaat aagaatagaa
    16141 aaagagagtg tgaaatcagc tagctattac taaaagctag ctgattgaac atcgttacag
    16201 gaaagagttt gagcctcact cttagcttcg attcataagg agatgtgaca taaattttgt
    16261 cgcatctctt ttttagtcta ctcttattat gtaggagaag gagggaggtg gatcatgtat
    16321 caagagtcaa taaagtagtt gataaaatcg ttcctctctc tgtttctttt ctctttgtca
    16381 tacttttatt tttcttatgt gttctatttg ttataataaa agagaaatgg gggaattgtt
    16441 gatgacgatt gaagaagcaa tagaatggat ccatagtcga ttaccatttg gttcacgtcc
    16501 tggccttgac cgagtcgaag ctttattaga agtggtaggg catccagaga gaaaagtaaa
    16561 aacgatccat atcgctggaa caaacggaaa aggttctact gtagcttatt tgagaagcct
    16621 attagaagag gctgggctga cagtaggtac atttacttca ccttatatcg aaacgttcaa
    16681 tgaacggatc gcaatcaatg gcaaaagcat tccagacaat gatttgatcc agcttgtaaa
    16741 aaaatatcag ccactgatca aacaacttga tcaatttgaa gcagtagctg gtattactga
    16801 attcgagaca ttaacagcaa tggcatttga ttattttttg atgaagaaag tggacgtagc
    16861 gattatcgaa gttggcttag gcggcttatt ggattctacc aatgtagctg taccaatgct
    16921 gacgggaata acaacgattg gacttgatca taccgaaata cttggagata caattgaaga
    16981 aatcgctgca caaaaagctg gaatcatcaa acaaggcgtt ccagtagtaa caggaaatat
    17041 tgccccagaa gcttttactg tcatccaaca aacagctaaa gcaaaaaatg caccgcttta
    17101 tgtatacggg gaaaattata aaataaacta tctgcatcct gatgagatgt ggggagaagt
    17161 atttgaattt tttaatgaaa taggaaagat ccctcaatta accactccgc ttttagggca
    17221 acatcaagct gaaaatgcag gagtagccat cgaattgtat tttgtttact gtcaattgga
    17281 aaagctgcct ttccgagaaa aagatgtttt gaagggattg aagaaatctc gatggccagg
    17341 aagaatggag cgtttgagta acgagccatt agttgtttta gatggtgccc ataatacgca
    17401 tgcaatgaac cggcttgttg agaatttgaa aaaagaattc aaaggatatc aaatcaacat
    17461 tttgttttcc gccttacgaa ctaaaaatgt tagagaaatg ctagaacgat tgcagcaagt
    17521 accaaatgtc catatttatt tgacgacctt tgaacatcca aaagccatcg aattaagtga
    17581 agtatcagat ttagcaagtg agagagtatc aatcgtttca ttgtggcaat tcggattagc
    17641 agatatattg gaaaagatga caaatgaaga cgtattagtt gtgaccggtt cactttattt
    17701 catttcagaa gttcgccatt tattaataca attaggagga gatcatgaag tataatgcag
    17761 tgatttttga catggatggt ttgctgttcg acacagagtt agtgtattac gaagccacac
    17821 aagtagtagc agacaagatg ggttttcctt atgataaaga cctgtattta aaattcttag
    17881 gtgtttcaga tgaagaagta tgggcgaatt atcatatgat tttctcggaa tttggcaaaa
    17941 caagtgtaca aagattcata gatgattcat accaggaaac gcttgaacgt ttttcgcaag
    18001 gagcggtaca aatgaagccc ggtgtgttgg aactcctctc ttttttagaa gaacatcaga
    18061 ttcccaaagt tgtcgcttcc agtaaccaac gagcagtcat tgaactttta ttgaagaaga
    18121 acaatctgtt tgaacgattt gagacgattg tttctgcaga aaatgttcaa cgagcaaaac
    18181 cagacccaga aattttcttg ttagctaatc agaagctagg tacagcgaag aaagaaacat
    18241 tgattttaga agattcacaa aatggtattt tagcagcttc tgcggctgag atcccggtag
    18301 tcatggtacc agatctgctt ccaccatccc aagaattgat caaaaaaaca gctggggttg
    18361 tttcttcttt acatgaaatc cctgcattgt taaaataaaa atccatctct tttcgtatct
    18421 tattttagat aagatagaaa ggagatgttt ttttgggaaa acgattgatc aaagaagtac
    18481 ctactagctc tttaccaagg gaacgtatgg aaatttatgg cgcggaagct ttatcggatc
    18541 aggagctttt ggcaatctta ctgcgtaccg gtcaacaccc ttatagtgtg atgtcgattg
    18601 ctggaaatct gttaaaaacg tttggaggac tggcctcttt acgacaggct actttgcatg
    18661 agttagaaga gattcgaggg ataggacgcg tgaaagcaat cgaaatcaaa gccttgatcg
    18721 aattaggtcg acgaattcag acggatcatg aaagagcggc tccaccggtt cgtaccagct
    18781 acgaattggc ccagcaattg attctggaaa tgaaagatca caaacaagaa catttggttt
    18841 gtatttattt ggacacgaag aatcaagtga tcttaaaaaa aacagtgttc atcggttcgc
    18901 tgaatcagtc agtcgctcat cctagagaaa ttttccatta tgctgtacgt tattgtgcag
    18961 caaggatcgt tttagcgcat aatcatcctt ctggtaatgt tattccatcg cagcaggata
    19021 tgaattttac caaaaggata caaaagtgtg gagaaatgat ggggatcact gtactggacc
    19081 acttgatcat cggacgcaaa cgatatttta gtttgagaga agaaggaatg atggaagaga
    19141 agtaagttgc tttcttgcaa aaaaaatgaa gcaagtgtac aattatattg taattttgtt
    19201 tgggaataga agttgaaaca tctgttgcac ctctatcgta cacgagcaat gttacaattt
    19261 aaattagtgc ttttggcacg aggaggatat tattatggaa aacggaacag taaaatggtt
    19321 caacgctgaa aaaggatttg gatttatctc acgtgaagat ggaagcgacg tattcgttca
    19381 cttctcagct atccaaggtg acggattcaa aacattagaa gaaggtcaag cagtgacttt
    19441 cgacgttgaa gaatcagacc gcggcccaca agctgcaaac gttgtaaaag cttaatttta
    19501 tctgtaaatc aaattgatgg tagacatcaa tttttacgag agaacaaagc gagtaacaaa
    19561 aatacctgcc acaagagaca atttctcttg tggcaggtat ttttttgtat gaaaaagtaa
    19621 ataaatttaa gaggctatga aagagccacc ttgtatcaag gaataagaac aaacaaaaat
    19681 ggcttctcat gtttttcatt tgttttctct tatattcagg acaaggtgct gatttcataa
    19741 cctcttattt attcttcatc tggtgtcatt accattggca aaatcattgg atgtctttct
    19801 gtacgttcga ataagaaagg ctgaagcgcg ccagtcatcg cttctttcaa tttatattct
    19861 gaacagtcag gtccattcaa acattctctt aaagcgtgga ataagattcg ctgagcttca
    19921 ttgatcattt ctcctgattc tcgcatgtag acaaatccac gagagagaat atctggacca
    19981 gccaagattt cttttttctt gatatccact gtcgcgacag ctaatacaag accttcttct
    20041 gaaagcaggt gacggtcacg taaaacgaca ttaccaatat caccgatccc gctaccatcg
    20101 atatacacat ctccagccac aaaatgccca gcaggacgcg cagaatcgct agttaacgct
    20161 aaaacatcgc cattttccat gatgaagcag ttttctgcag gaacacctac atcttgtgct
    20221 agagaagcat gtactttcaa catgcggaat tctccgtgga caggaataaa atattttggc
    20281 ttcatcaaac gaagcattaa tttttgttct tcttgtccac cgtgaccgga agtatggatg
    20341 ttattgattt ttccgtgaat cacttctgca ccagcttcag acagcaaatt gatcaaatga
    20401 ttgacacttg ttgtattacc aggaataggt gaacttgaaa agatcactgt atcgtctggc
    20461 tggatttgga tttgacggtg agtaccattc gcgatgcggc tcaacgcggc catcgcttct
    20521 ccttgagaac ctgtacatag gatcatcgtt tcattggcag gcaagcgatt gatttcgtgt
    20581 gcatctacaa atgtgccgtc tggaactttg atatatccta aacgttgacc gttgacgatg
    20641 gcattttcca tgcttcgtcc gaaaacagcg attttacgac cagttgccac agcggcatct
    20701 gctgcttgct gcaaacggaa gatgttagaa gcaaagctgg caaaaatgat acgtccttcg
    20761 atttttgaaa agatcttcaa aatagaatgc ccaatcgttt tttctgattt agtaaatgtt
    20821 ggaatctcag catttgtact atcagataaa aggcacaaga ctccttcttc cccgatacga
    20881 gccattttat gcaagtttgc tggttcgcca acaggagtga aatcgaattt gaagtctcca
    20941 gtcgctacga tatttccaga aggtgttttc accacgatcc ctaatgcatc tgggatacta
    21001 tgggtcgttc tgaaaaagct gacagaagtc tttctaaaac ggatgatcgt atcttcattg
    21061 atctcatgga gctctgcatc tcgaagcaag ccatgctcat ctaatttgtt tgtgatcaac
    21121 gctaatgcga gaggaccagc atagatagga atatttgctt gtttcagcaa gtagggtatt
    21181 ccgccgatgt ggtcttcgtg cccgtgagta atcaccaagg cttttacttt gtgtaagttt
    21241 tgtatgatgt agctgtagtc gggaatgaca tagtcaattc caagtaaatc gtcttccggg
    21301 aatttgatcc cggcatcaat caaaatgatt tcatcttgga attgtacccc atacgtattt
    21361 ttcccgattt cacctaatcc accaatagca aagacgcctg tctcgttatt tttgatggaa
    21421 agtttcatat taaaactcca ctaatgagaa atcagcattt tctttttcgt actctagatg
    21481 acttccttct agaagttgaa cgaattcaat attgtatggg gtattttctt ccacacgttg
    21541 gcgagcgtct acatcgcttt ttgcctcaat gtaaagagat tttgtttctt cccgtttagg
    21601 gttgcgtact tttgtttctt gataataaac tttatagatc attgttttgt ctcctttatc
    21661 tgcgtaaagc agtgatttag ctagttgatt tttttagaca acacaaaacg aggggacaag
    21721 ccacctggtt tttcatatgc tttgactaga atactggatt cgagtcaaag agacaagcct
    21781 cgtttttgct tatctgttcg gatatttttt caaccgtacc ttctgtataa aaaatataca
    21841 acaattaatt gttattctag cataaaacag caaataagta tagaaatcaa ctagattgaa
    21901 agaaaaaatc aatgtttatc atcccgctat aatcataatg acttttttat gagagaagtg
    21961 ttctattaat aggctcgttg tgctatcatt tttagtaatg aaaatgtaac aaaagattaa
    22021 aaggagatga gaaagtgaaa ctaatgtggc attataccat gcgttataag aaattcttgt
    22081 ttttcaattt catatgcgta tttggtttta ttttgattga attaggactt cctacgattt
    22141 tagcacgtat gatcgatgta ggaatccgca atgatgattt tgattatgtg aagcaacaag
    22201 ggatatttat gatcgttatt actgtaatcg gtattatttt gaatatcttg ttaggttatt
    22261 ttggttcgag gattacgact aatatcgtag cagatattcg agatgatttg ttcgaaaaga
    22321 tccaaagtta ttcgcatcag gaatacgaaa atctaggcgt atcttcattg atcactcgta
    22381 caacaaacga tgcttatcag atcatgttgt tcctgcaaaa tattttacgt atcggtttta
    22441 tggcaccaat gatgttcatt gtcagtttgt acatggtcat gcgtacaagt gtgacactca
    22501 gtttgtatgt agtaggtgca ttacctttac tattattggc agtcgtagcg attgctaagg
    22561 tgtctgaacc gttatctaaa aagcaacaaa aaaacttaga taaaatcaac agtatcttgc
    22621 gggaaaatct ttcaggattg cgtgtgattc gtgcattcgt caatgaaaag tttgaagaaa
    22681 aacgttttgc tgtagtcaat gagaattatg cttctagttc aaaaagctta ttccgtttga
    22741 tggctgttgc acaaccgggt ttttatttct tatttaatat cgtaatggta ttgatcatct
    22801 ggagcggggc agtgcaaatc gatgcaggcg agcttcgtgt aggagatttg attgcgttta
    22861 tcgagtatat attccatgcc ttgttctcat tcatgttatt tgctagtgtg ttcatgatgt
    22921 atccaagagc agctgtttca gctggacgta tccaagaagc cctagatgcc aaaccttcga
    22981 ttgtagaaga tccaaatggt atatctgata caaaaactaa agggtatatc gaatttcaaa
    23041 atgtaacatt tgcttatcca ggccattcgc aagagccagt gatacgaaat gtcagtttta
    23101 cagcctctcc aggagagacc gttgctttta ttggaagtac cggtagcgga aaatctacgt
    23161 tgatccaatt gatccctcgc ttttatgatg tgagtgaagg aaagatcttg atcgatggag
    23221 tagacgtgag agattatcgg ctaagcaagt tgcgagataa gattgggttt attccgcaaa
    23281 aagcgctgct atttactgga acgattgcag ataatttgcg atacgggaaa gaagatgcta
    23341 cgcaagaaga aatggaacgt gcagctgaga ttgctcaagc ttcagaattc atttctcgaa
    23401 aaccagatgg atttaatgaa catttatcag aaggtggttc caatttttca ggtggacaaa
    23461 aacaacgatt ggcaatagcc agagccatca tacgccgtcc ggaaatctat atttttgatg
    23521 acagcttttc tgcattagat tatcaaacgg atgcgaagtt acgtgctcgt ctgaaaaaag
    23581 aaacgacaga atctactgta ttgatcgtag cacaacgtgt gggaacgatc atgcatgcag
    23641 ataagatcat cgtattaaat gaaggagaag tcgtcggtat ggggacgcat cgggagttgt
    23701 tggaaaaatg cccgatttat tatgatattg ccgcttctca attatcaaag gaggaattag
    23761 catgaaaaaa accttttctt ccttaaaacg tttagctcga tatatcagac cgtatcgtga
    23821 gacttttctt ttagtgatcc tatttacagt tctgacagtg gctttcaacg tggctttgcc
    23881 atatgttaca ggacttccta ctacagaaat cagccgtaat attgccaatg gcgaatcaat
    23941 caatttttct tatatattca aatgtctcat ttggatcaca gttgttggaa tagggtattg
    24001 tatttcgcaa ttactttctg gtgttttgat gacaaatgtc gttcaaagtg ccatgcatga
    24061 tttgagaaga gacatagaag aaaaaatcaa tcgtcttccg gtttcatatt tcgataaaaa
    24121 ccagcaagga aatattttgt ctcgtgtgac gaatgatgta gatgcagtca gcggtgcttt
    24181 acaacaagct tttattggta tcgtaaatgc gatattggga attacgttgg ctgcagcaat
    24241 gatgttttat atccagccga tgatggcgct gatctctatg atcatgattc caacatcgat
    24301 ctggatctca aaacgagtga tcaatgcttc gcagaaatat tttcaaagca tgcagaattc
    24361 tcttggtgag ctaaacggct acgtccaaga aaatatgaca ggttttagtg tattgaaagt
    24421 atatggacgc gaaaaagaaa cattggaagg attcaaaaaa gttaatcaca gcttgaaata
    24481 ctttgggttt cgtgcagcat tcatttctgg cttgatgatg cctctagttc aattaactgc
    24541 ctatgctact tatattggta tggctgttct aggaagcttt tatgttatta ctggggcgat
    24601 cgttgtcggt caactacaag cattcatcca atatatttgg caaatcagtc agccaatggg
    24661 gaacgtgaca caattgtctt cagttttgca aagtgcatca gcggcaacga aacgtgtatt
    24721 cgagatttta gatgagccag aagaaaaaga aaacaaagta gatgcttcac tgccagaaac
    24781 gattttagga gatgtgacat tcgatcatgt cgattttgca tatgatccga agaaaccatt
    24841 aatccaagat ttgaattttg aagtgaaagc tggtcaaacc gtagctgtgg tcggtccgac
    24901 cggtgccggc aagacgacat tgatcaatct attgatgcgt ttttatgacg tagacaaagg
    24961 agcaataagg attgatggaa tcgacaccaa atcaatgagt cgcagtgatg tgcgttcgct
    25021 atttggtatg gtcttgcaag atgcatggct ttatcaagga acgattgcag ataatatccg
    25081 cttcggaaag cttgatgcga cagattatga agtagttgat gctgcgaaaa cagcaaacgt
    25141 agatcatttt atccgaacga tgccagatgg atatgatatg gccatcaatt ctgagggaga
    25201 caatgtgtct ttaggacaaa aacagttgct gacgattgcc cgagcagtgg tctccgatcc
    25261 taaaatcttg attttagatg aagcaacaag ttcagtagac acccgtttag aagcgttgat
    25321 tcaaaaagca atggatcgcg tgatggaggg acggacaagt ttcgtcatcg ctcaccgttt
    25381 atcgacaatc cgtgatgcag atctgattct agtgatggat caaggtcaga tcattgaaaa
    25441 aggaacgcat gagagtttat tggcacaagg tggattttat gaaaaactgt ataacagcca
    25501 atttgctgag gaagcagaat aagtcatata agaataaaac agtttaaaac ttattttttg
    25561 ttctctattc ttgataatgg taaagtaaag ttatcaagaa aagtgaggac acggagatgg
    25621 aacagttttt aaatgaaaaa attactgaag ccaaaagaga agttgatcgt ttaacgcacg
    25681 tctgtcagac gaagcctttt gattcagctg atttttttga aaatgagttt caaatcgaac
    25741 gcttggcagc acaaatagaa gtgtacgaat taattttaaa aaaagtaata gaagaaaata
    25801 tagatgtaca ataaaaacag gtaaaacgtc agttaccaga tactgtaaat agacatctgg
    25861 cctgagcagg ttttacctgt tttttttctt atgaaataaa tatatgctat gataccttta
    25921 ttcaatagga ttagtttcta caaaaaatga aaggagaaaa ataaatatgg aacggtttat
    25981 agcatcaaat gccacagtga ttggtgatgt gacattgtca gaagatgtga cgatctggta
    26041 tcaagctgtt ttacgcgggg acagtaattg gataaaaata ggacaaagaa cgaatatcca
    26101 agatggtaca atcatccatg tcgatcatga tgcacctgta gatattgcgg agaatgtgac
    26161 ggtaggacat cagtgcatgc tacatggctg tacaatcgaa aaaggggcgc tgatcgggat
    26221 ggggaccacg atattgaatc atgcggttat tggtgagaat agtttgattg gtgctggatc
    26281 attagtaaca gaaggaaaag ttattcctcc taatgtcttg gcttttggtc gcccagctaa
    26341 agtgatccgt cctttgacaa aagaagaaat acaaaaaaac aaagaaaaca tccagcatta
    26401 tgtcgagata ggtcggatgc atgcgcaagg aaaattcaag gagcaaagat aggcagtagc
    26461 atccataaaa taaaaaagga tggtcagttt gagttgacca tcctttttta tttggtttag
    26521 ataagagagc ttaaaaaagc ttcctttgaa acagattaat ttaatgaaga actgttttcc
    26581 atgacttcat cgatcaatcc atatgctttt gcttgttcag ctgtcatata gttgtcacga
    26641 tctgtatctt tttcaatcac ttcaagcggt tgtccagtgc gttcagctaa aattttgttt
    26701 aagcgttcgc gtgtttgcaa aatatggcgt gccgcaattt cgatttcagt tgcttgccct
    26761 tgagcgccgc caagaggttg atggatcatg atctcagcat ttggtaaagc aaatcgttta
    26821 ccttttgtac cagctgtcag caagaagctt cccattgaag cagccatacc cataacaatt
    26881 gtttggacat ctgctttgac aaagttcatt gtgtcgtaaa tagccatacc tgctgtaact
    26941 gaaccaccag gagaattgat ataaagatag atatcttttt cagaatcttg agcgtctaag
    27001 aaaagcaatt gggcgatgat cgaattagca agatcatctg ttacttggcc gcttaacatg
    27061 atgatacggt ctttcaataa acgtgagtag atatcgtaag ctctttcacc tcgtgaagat
    27121 tgttcaatga ctgtaggaat taaattcata atagagttcc tccttgattt cgtttagaaa
    27181 tagtgtacca taatttagtt tctaatacct aaaatgtagt atacattttt ggtcaaaaaa
    27241 ggtcaaataa aaaacttagg caaattagtc gcataaaccc gctttatggt atgataagca
    27301 gagaaacaaa acaaggagga acaacagatt gatagttggt attactggcc ttatcggagt
    27361 gctgacaggt gctgtagttg tctatatcgg catgatactg caaatgcgtc acttggaaaa
    27421 taaagaacag cgtagacgtg aattggaatt gaaagtcaaa gaaatcgaga cgctgaatca
    27481 attaaataaa aagatcaacg agatcttgca aaaacggaca acgctgatgg aagaatatgt
    27541 cagctttgat gcatttgatg attgttatat cacgattgac gattttgcgt atctccaatc
    27601 attttcagcg caaaatagtt tttatctgcc aaattatatt ttagacgagc tatttaagaa
    27661 tatctcccat cgaaaagtga ttttatctcc agatgaaaca atgaagattg gtggatatgc
    27721 ttataaaggc ggacgcgtga ttttagagaa ttttacggat agtctgactg aaatgatcaa
    27781 tgaaaaaaaa gctcagttga agactatgac gcgccagccg attggtttct tctcgaaaga
    27841 tgatgtttgg agctgatatt ttgaagcatt gccgttgcac atgagagcgt ggtaatgctt
    27901 tctttttttc ttaatgatac aatagaactg aacggagtga agaaagatgt tacaaatcta
    27961 tgcccatcgc ggaagcagcg gcacccaccc agaaaatacg cttcctgcat ttgcagaagc
    28021 tgtacgagta ggtgcagatg gtatcgaact agatgtccat ctaagcaaag atggttattt
    28081 gatcgttatg cacgacgaag aagtggaccg gacgactaac ggtaaagggc tgattcgaga
    28141 aaaaacattg gaagaactca aaaaattgaa tgcagggggc aaattcagca aagaatttcc
    28201 agcagctaaa gttcctgaat tgagagaagt gttaggactg ctcctccaaa aaaattaccg
    28261 agggatgcta atgattgaaa ttaaaactga tcagtatgaa tatcccggta tcgaagaaaa
    28321 attagccgaa acattaaaaa gtagagattg gccttttcaa catgcatatt gcagctttca
    28381 tttggattct ttggaaaaaa tcgcattact ggaaccggag atacagctgg acttattgat
    28441 gagtacttct gataaaaaga tccaactagc agcagaaacc ccttatatag aaggagtaca
    28501 tccaaaaaat acttggttgt tcgaacatcc agtccaggca gctgattttc caaaagcaat
    28561 tcgtgcatgg acgattaacg atgaagaaga aatggccaaa tgtcaagaac tcaaaataga
    28621 tggaataata actgattttc cggagaaagc tcttcggtgg aaacaatcaa cgaaaagtag
    28681 gtaaaatatg gaaaaagtat tagtgatcgt cggtccgaca gcagtcggta aaacagcgct
    28741 aagtgtggaa ctcgcacaac tatttggagg agagatcatc agtggtgatt cattgcaaat
    28801 ctataaaaat ctggatatcg gtacagcaaa aatcaaagaa gaagagatga ataatgttcc
    28861 gcatcatttg attgatgtga tcgaacctac tcaacgttac tcagccgctg attttcagca
    28921 ttcaggtcga caattgatta ccgagattgc gaaccgaggg cacctaccta tcatagctgg
    28981 tggtacgggt ctctatatcc aatcccttct atatgattat cagttaggag cgacagaaga
    29041 agcgaatcct gagattagaa aaaagtatga aacgttagct gaaacaattg gttcccaagc
    29101 attatggaat cagttgaaag aaaaagaccc ggcagctgct gagaaaatcc actggaacaa
    29161 tcaacgcaaa gtgataagag ctctggaagt tttagagacg acaggacata gtatcacggc
    29221 acctaaagaa gagccaaagc aattgtatga ttacctcatg attggattag atacgaagcg
    29281 tagtattctt tatgaacgaa tcaatcaaag agtcgatttg atgctagcag aaggtttagt
    29341 agcagaagcg cgtaaggtgt atgagcttgg agaagtccag gccagtcaag gtattggata
    29401 taaagaattt ttcccttatt ttgaagggaa agaaacattg gaagaagcaa cagaacaagt
    29461 caaactacat tcgagaagat acgcgaaacg tcagttgacg tggtttcgta accgtttgga
    29521 tgcacagtgg tttgatctta taagtgagcc aaatcaaaaa agtcagattg agcaaacaat
    29581 caaaaaatgg ctggaggcgg aataaaatgg aagaaaaagt gatcatcgta ggggtagaaa
    29641 ccgaagcaaa ccagcgttac ttttcagagt caatggaaga gctagttcag ctgacgaata
    29701 ccgcatcagg agaagtcgta ttcacaatta cacaaaagag accacaagtg gatcggcaaa
    29761 cgattatcgg taaagggaaa ttacaagaat tagttcaaca agcggatgcg catgaagcgg
    29821 atttgatcat ctttaatcat gagctaactc cgagacagag ccaactgatt accgatgctg
    29881 ttggtcttcc ggtaattgat cgcgtgcagc ttatcttaga tatttttgcg atgcgtgctc
    29941 gttcgaaaga agggaagctg caggttgaac ttgctcagtt agaatacctg ctgcctcgtt
    30001 tagtagggca agggaaaaca ttatctcgtc ttggcggtgg gatcggaaca agagggccag
    30061 gtgaaacaaa gttagaaaca gaccgccgac atatacggaa taaaattttt gctgttaaga
    30121 aagaattgaa agaagtagaa gctcatcgag agcgcaatcg ccaaaaaaga aaaaatagcg
    30181 agatttttca gattggactt attggctaca cgaatgccgg gaagtcaact atcttgaatc
    30241 tattgacaca agcggatact tattcgaaag atcaattgtt tgcaacgtta gatccattga
    30301 ctaagagatg gcgttttgct gaaggatttg agattactgt aacggatacc gtcgggttca
    30361 tccaagacct gcctacgcaa ctgatcgatg ctttccattc tacattagaa gaaagtcaaa
    30421 atatggatct tttgctacat gtagtcgatg ctagttctcc agatcgtatt ttacaggagc
    30481 agactgtctt aaaattgatg gatgaactga atatgaaaga aatgccgata ctgactgttt
    30541 acaataaggc agaccagatc gacccagcga tgttcacgcc tacgctattc ccaaatgtat
    30601 tgatttctac tcagacgaaa gaaggtaaga tggcgttgat cgaagcaatc aaacgtcagc
    30661 tgatggaact aatgacccct tatacacttt ctgtgccaag caacgaagga cagaaattaa
    30721 gcgagttgag aagacaaaca atggttttgg acgaagcgta catggaagaa acaaacgaat
    30781 atgaagttaa agggtttgca aaaaccaatt caaaatggtt aagaaaaaac caataaaatt
    30841 ttattttttt aa
//
LOCUS       DASJTC010000024        30773 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.024, whole genome shotgun sequence.
ACCESSION   DASJTC010000024 DASJTC010000000
VERSION     DASJTC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.024"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(11..1594)
                     /locus_tag="U4B19_000985"
     CDS             complement(11..1594)
                     /locus_tag="U4B19_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2076743.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(2172..2315)
                     /locus_tag="U4B19_000986"
     CDS             complement(2172..2315)
                     /locus_tag="U4B19_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEN2076744.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(2538..3017)
                     /locus_tag="U4B19_000987"
     CDS             complement(2538..3017)
                     /locus_tag="U4B19_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2076745.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(3090..3374)
                     /locus_tag="U4B19_000988"
     CDS             complement(3090..3374)
                     /locus_tag="U4B19_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076746.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(3452..3646)
                     /gene="ssrS"
                     /locus_tag="U4B19_000989"
     ncRNA           complement(3452..3646)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U4B19_000989"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(3718..5004)
                     /locus_tag="U4B19_000990"
     CDS             complement(3718..5004)
                     /locus_tag="U4B19_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEN2076747.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            5142..5813
                     /locus_tag="U4B19_000991"
     CDS             5142..5813
                     /locus_tag="U4B19_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEN2076748.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5779..6267
                     /locus_tag="U4B19_000992"
     CDS             5779..6267
                     /locus_tag="U4B19_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEN2076749.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            6280..7227
                     /gene="prmA"
                     /locus_tag="U4B19_000993"
     CDS             6280..7227
                     /gene="prmA"
                     /locus_tag="U4B19_000993"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEN2076750.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            7228..7992
                     /locus_tag="U4B19_000994"
     CDS             7228..7992
                     /locus_tag="U4B19_000994"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEN2076751.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            8225..10438
                     /locus_tag="U4B19_000995"
     CDS             8225..10438
                     /locus_tag="U4B19_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEN2076752.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            10449..10895
                     /locus_tag="U4B19_000996"
     CDS             10449..10895
                     /locus_tag="U4B19_000996"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEN2076753.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            11066..12325
                     /locus_tag="U4B19_000997"
     CDS             11066..12325
                     /locus_tag="U4B19_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEN2076754.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(12414..13361)
                     /locus_tag="U4B19_000998"
     CDS             complement(12414..13361)
                     /locus_tag="U4B19_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2076755.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            13755..15047
                     /locus_tag="U4B19_000999"
     CDS             13755..15047
                     /locus_tag="U4B19_000999"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEN2076756.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            15062..16831
                     /gene="aspS"
                     /locus_tag="U4B19_001000"
     CDS             15062..16831
                     /gene="aspS"
                     /locus_tag="U4B19_001000"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEN2076757.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            16940..17815
                     /locus_tag="U4B19_001001"
     CDS             16940..17815
                     /locus_tag="U4B19_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2076758.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            18081..19964
                     /locus_tag="U4B19_001002"
     CDS             18081..19964
                     /locus_tag="U4B19_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN2076759.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(20039..20323)
                     /locus_tag="U4B19_001003"
     CDS             complement(20039..20323)
                     /locus_tag="U4B19_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEN2076760.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(20313..21047)
                     /locus_tag="U4B19_001004"
     CDS             complement(20313..21047)
                     /locus_tag="U4B19_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEN2076761.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            21176..21799
                     /locus_tag="U4B19_001005"
     CDS             21176..21799
                     /locus_tag="U4B19_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEN2076762.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            21991..23112
                     /locus_tag="U4B19_001006"
     CDS             21991..23112
                     /locus_tag="U4B19_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEN2076763.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            23119..26247
                     /locus_tag="U4B19_001007"
     CDS             23119..26247
                     /locus_tag="U4B19_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEN2076764.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            26266..29460
                     /locus_tag="U4B19_001008"
     CDS             26266..29460
                     /locus_tag="U4B19_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEN2076765.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(29606..30313)
                     /locus_tag="U4B19_001009"
     CDS             complement(29606..30313)
                     /locus_tag="U4B19_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN2076766.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        10066 a         5579 c         6512 g         8616 t
ORIGIN      
        1 aaaaaacgtc ctagtcaatc agttggccga gcggctggcg gctgatctgc tcctgatcaa
       61 taacgagtgc ttcttggaag tgttgctgca aaaagtagcg gtcatggctg actgctagga
      121 gcgttccttg atagtcttgc agcatttctt caatttcttc tcgagcatag ctgtcgaggt
      181 gattggttgg ttcatccaaa atcaggacgt tgattttttc taaaaagagt ttcagcaacg
      241 ccaaacggat tttttcacca ccggataaat ctttgatccg tttacccaca tcgctttgat
      301 aaaagccact tttggctaat tgccgtcgag cgacttcttc gtttccagtc aatgtgatgg
      361 cgtaagccag caaacggcta tcgggatcgg caaactgcag ctgctgcggc aaataaccga
      421 tttgcaagct actacctaat ttgatggtgc caccgtaagg cttttcctca ccgagtaata
      481 atttgagtaa ggttgatttt cctgcaccat tgtcgccgat gatggctaaa tgatctccac
      541 gatagactgc aaaactgctg tctttgaata gtacttgatc agcgtactgc tggtgcaagt
      601 tttgaatgat gaaaacttct ttgccagatt ttccaccatt agacaagctt tgcagccgat
      661 ttttcggggg aggtggcggt ttgacgagtg tcagtttcgc caggcggcgt tccagttctt
      721 tggctttttt gaagaaggac tcattgtctc cttcatgtgc ccactgacga aaacgacgaa
      781 tcatgacttt caaccgctgg acttcttttt gctgcagttc gtaatttttt gtcagctcag
      841 ccaagcgagc ttctttcaag actcgaaaac ggctgtagtt tccgggatac tcaatcaatg
      901 caccttcttc cagttcccat gtttttgtgg tgacttgatc gagaaattgc cgatcatgag
      961 aaacgaccag aaccgatttt tttgtattct gtaaatagtt ttccaaccag cgaatactgt
     1021 taagatctaa atgattggtc ggttcatcga gtaaaagtaa atcggcatcg gctgctaaaa
     1081 ttttggctaa agcgacttta actcgttcgc ctccgctaag ttctgttaac cgtgcttcct
     1141 gacggtgtgg aatccctaag ccttgcaaga tcgctgcaat ccggtcttct aatacgtaac
     1201 cgcctagttc ttcaaagcga tgctgcagct caccgtaacg ggccagacac ttctctaagt
     1261 ttgtatctgg ttctgtcatc aatgcttcgt agcgactcat ttgctgtttg aggtcctgta
     1321 tctcagcggc agaatggagc aagtagtggt tgacggtcgc ttcggaaact gtaagttctt
     1381 gttcgaccgt cccgatcttt aagccttttt tgcgactgat aacaccgctg tcgacacctt
     1441 ctctgccggt caagatttgt aaaaaggttg atttgccagt tccgttgata ccgactaaga
     1501 caattttttc accagcttca atttgacagg ataaatcctc aaataatgga ttcccgccga
     1561 ttgttttttt aatagaaatt gcttgtatga tcatgatctc tctccttgag accactcaaa
     1621 aaggccatgg ttatccatgg cctgtgttat ttaaactcag attatcatga gaaaaatacc
     1681 ttgagatggc cattgatttt acgtgaattc aactgttttg gacaaacttg tcccgtcaac
     1741 agcaactgta aaaccaattg catggctaaa tgcgatatgg tatcgttggt aattttcatt
     1801 acttgttatt ttagtcatgc gcatcatagt attttccctc cttcgtgtag ttgtcttttc
     1861 cagtgtacct gaaagtgtca gaaagttcaa ctagcgctga catttactta atatttatcg
     1921 atgtgtaatt ctgtcagaga gcttttttta tctgactgag ggtaatgtct cgtggaccac
     1981 atcattgtat tctgagctaa accagcgttc aggcgaaatc atatggtgtt tgataaaaaa
     2041 ctttgtctac atgacttcct tttgccattt gcttaatacc gttgcattaa acagctcttc
     2101 tctttggaaa tctcaccaac atcgcgttca ggagtatcgt tgtggttgca atggcaagcg
     2161 gtagcctttc atcaattctt gaatgattta ccgaatgtag cacttgcctt ctcgcggtac
     2221 ccgaacccga aataaaattt tagtttggcg ataaaatcgg tatccgccat cgatacttca
     2281 tattttttcg tatcagattc atcggaaact tccacattga cgaaccaata agctttggca
     2341 tgcttaggtc gttttaaacc acaccagggc gactaatcgc cctggtgttc actttttttt
     2401 attttacttg aactcattga ctgttttccc tttattttaa acattataaa gaccgatgag
     2461 tgcttgccgc gtcttttgtt tttatcacgg ttttttgtgc aaaaaaacct gccgatagtt
     2521 ggcaggtcgt ctacgtgtta agctttggtg tggtctttag ctggcaattg gttttctaaa
     2581 tcagttcgga caacaagcac atcgcaagct gcgtttctaa taacatattc agaaacagaa
     2641 ccaataaata aacgttctac agcattcaat cctgtggctc ctaacataat caaatctact
     2701 tgattatctt ctgggatttc tctggcaata atttgttttg gtgagccata ttctacgaca
     2761 gaggttacat tattcaagcc agctttcttc gcattgcttt cataatctgc caatgtttgc
     2821 tttgccattt cagtagcttg ttctgcaagc attccatcaa aggatgaaac ggtttggaaa
     2881 gaacgtgtgt caatgacatg tgctaataga agttctccgt tatttcggat agctacattg
     2941 accgcctttt taaaagctaa ttcagcttca tcagaaccat ctacggcaac catgattttt
     3001 ttgtattgtt gtaacataag cattcactcc tcaagtcaat cgtattgtac tcttttattg
     3061 tagaacactt tagggaaaaa gagaattatt tactgctttg gttcagcaat tttttttgca
     3121 aatcggatac tgaaggtggc agaaacaata attacgatca gtaaaaagac caaggcaata
     3181 aagcgttgat tgaaaaaagc tgttaaaata cccattacgc ctaaaacagc atatgcagct
     3241 ccgtaaaatt gtaggaatcc ttgattttta tcatttttgt tgatcaatac aaaaaagata
     3301 tcctgtttat taaataaata atagctaacg aataataata ctaaagtggc gataatcatt
     3361 aaaatcctaa tcatgaacaa atcctccaaa ataagcaaca aatagaaaaa gcaatttgcc
     3421 aagagcaaat tgctttattc tttaaaagat ggtaatctcc gttaatgccg ttgtttgtcc
     3481 tgtagtattg atcccgcgaa ttcaagcagg tgggtcccat ggtcgtagct tcgaactacc
     3541 aaatgataag gcatgttacc agcagtgaca caagacgggt cccaagatat caataaaaat
     3601 gttcggtcaa cacacaaaga ggacaattcg aacatcacag atttcccacc tctatagtag
     3661 catatgaatt taaaaagagc aatgagagag ctcgcagaaa cagaaccgtt ttcttgtcta
     3721 gttttttgga ggatgctttt tccactcttt tatacggtaa tactgttgac ccagagcctg
     3781 ttcatatttc cctgtatctt ttggctgata atactgagca ttcttcagtt tatctggtaa
     3841 atattgctga ttgacccaag cctgatcaaa atgatgagga tactgataac ccacgccacg
     3901 gttgagtgat ttcgcacctt tgtaatggct atctctcaaa tgatcaggaa catctccagc
     3961 tttgccctct cgaatatcag caagtgctgc atctaatgcc atgtaggcag aattggattt
     4021 tggcgacaag caaagatcga cgacgacgtc tgctagtggg atacgtgcct caggcaatcc
     4081 caatttttca gcagctaata cagcgttcac tgtacgagcc gcagcagcag gattacctaa
     4141 acctatatct tcataaccga taaccatcag acgccggcaa atacttgcca gatcacctgc
     4201 ctcaactaaa cgggcgagat aatgaagtgc agcatccaca tcacttccac gaatcgattt
     4261 ttgaaaagcc gaaataacgt cataatgagc atctccattt ttgtcatggg taagagcttt
     4321 tctttggaca cattcttcaa tgatcgacag tgtaagatga atccttcctt ctttatctga
     4381 aggagtcgat agtgtagcca gttccagccc attcaatgca ctccgcaaat cgccgttcgt
     4441 tgcacgcgaa aggtgaagaa gagcatcttc gtctaactgg attgcttgtt gtccaaggcc
     4501 gcgttcttta tcccttaaag catgttcgac tgccaactgg atgtcttgtt ctgttaatgg
     4561 tttgacttca aagatctgtg tacggctgcg gatcgctgga ttgatggtaa tataaggatt
     4621 ctcagtagtc gctccgatca agatgattcg gccactttct aaatgaggaa gcagaaaatc
     4681 ctgttttgtt ttatctaatc ggtgtacttc atccaataag aggatcactg taccactcat
     4741 ttttgcttct tccgctacaa cttgtagatc cttttttgaa tctgtagcgg cgtttagcat
     4801 gcgaaatgca taattagtag agccggcgat cgcacttgcg atactcgttt ttccagtacc
     4861 aggaggtcca tatagaatca tggaagagag catgcgagca tcgaccatgc ggcgtatgat
     4921 ttttccttcc ccgactagat gctgctgacc aacgacttca tcgatcgttc gaggtctcat
     4981 gcgataagcc aatggctgtt gcataggatt ttcctccttc ttgtttttac gtcccttaat
     5041 tatatcatat ttagcaagat gcctagaaag aacagccttc tgttgttttg ttttcatagc
     5101 ttatccaact aatattgtgt atgataaagt aggtgagaaa aatgacggat aaacaaaaga
     5161 tagatgaatt tttttggaat aaaacgacgc cagaagtggc acgggattta ttggggatgt
     5221 atttggagca tgacacacca gaaggaagat tagctggata tattgtagac gcggaagcat
     5281 acctaggtcc agaagacgag gccgcccata gctatggttt aagaagaaca ccgcgtgtgc
     5341 gggcaatgta tgaaaaacca ggtacgatct acctttacac gatgcatact catcggattt
     5401 tgaacatcat tactcagcca gaagggattc ctcaaggagt aatgatacgt gcaatcgaac
     5461 ccgccgcaat gattgatcaa atgagtaaaa atcgtggcgg aaaaactggt ccagatatca
     5521 gcaacggtcc gggaaaatta gtggaagcat tagcaattcc tcaagaactt tatggtcagt
     5581 ctatagcaga tagttcgtta cgcttagttt ttgagaaaaa aaagacacca aaaaagatca
     5641 atgctttgcc tagaatcggt atcccaaaca aaggtgtatg gacagaaaag ccgttgcgtt
     5701 ttgttgtgtc gggaaatcct tatatctctt tacaaagaaa aaaccaaatc gagaaagatt
     5761 ggggttggag gaaagaaaat gaaaaagaag gatctattaa catatttaga tgaaataatt
     5821 gaaaaacaag tgacagacta tgatgtagct ttagattggg atacaaaaaa tcatacgatc
     5881 gagatcgttg ttcgcttgtt tgcagaaaat aaagcacaat ttgagattga tgatgcagaa
     5941 gggatcgttt cgcaagaacc aattatcgaa tttgaagacg gcgtacttct ttttaatcct
     6001 cagaaatctg tttttgatga acgcaattat ctagcagtaa tcccttatga aggaaaaaaa
     6061 gggttggcaa aatcagtagc tgattcttta gtggaatatc taaatgaagt attagctcaa
     6121 gggcaaagcg atttgcttga ttttttggat gaagacgatg acgaagctgt attcgagctt
     6181 cactgggata atcagcaact agcagaatta gtagaagtaa aacagcaaaa tgaagcagat
     6241 acttatttac cgtacccaag ttattaagga gtgacagata tgaaatggaa tgaagtaaaa
     6301 gtagaaactg aaagtgaagc agtcgaagca gttgctaata ttttgatgga ggcaggggcg
     6361 agtggtgtcg cgattgaaga ttctttagat gttgaacatt ttcagtcgga tcaatatggt
     6421 gagattttag ataaaaatga ttttacatcg ctgaaagaag gcgcactagt catggcttat
     6481 tttccggaaa cgactttctt accggaaatc ttgcctttta tcaaagatcg tatcacacaa
     6541 ttacctgagt ttggtctagc tattggaaaa aataaagtaa cagttagtga agtagaggaa
     6601 agcaactggg caacagcctg gaagaaatac tatcatccgg tgcgtattac gcgaatgctg
     6661 acggttgcgc caagctggga aaactaccag acagaagatc ctctagaaaa aattatcaca
     6721 ctagatcctg gtatggcatt tggcacaggc actcatccca cgacttcctt gacattgaca
     6781 gcactagaaa cagtcttgcg tggtggtgaa acattgctag atgtgggaac tgggtcgggc
     6841 gtgctaagta ttgcagcaaa atatcttggt gcaaaagatg tctatgcctt tgatttagac
     6901 gaagtagccg ttcgttcggc gaaagaaaat atggatatga atgaaatagc caaagatgtc
     6961 catgtatcag ctaacgatct tttgacagga atcgagattg aaagtgatgt aatcgttgcg
     7021 aacattttag cagatatcat cttattgatg atcccagatg cttggcgttt actgaagcaa
     7081 acaggcacgc tgatcgtttc aggaatcatt gaagagaaga agcaaatggt tctggatgcc
     7141 atgttagaac aagggtttat cgtggatcaa attttccagc aaaaagactg gtatgcaatt
     7201 attttgaaaa aaccagaggt ggaatagatg cagcggtatt ttttaaaaga atcatatgaa
     7261 tcaaaagact attttgtttt agaaggggaa aactatcacc acattgtacg ggttatgagg
     7321 atggaacccg aggatgaagt ttttttggca ttcaatgatc aaatagccat tgttgcagaa
     7381 atcacggaga ttactgaaga ggctgtttat ctgaaagaaa ttaaaaaaga agaaaatcaa
     7441 aaggaactgc caagttcgat taccatagcg agtggctatc ctaaagggga aaagctggat
     7501 tggatcgttc agaaatccac tgaattagga gcgcaagctt ttcttggttt cccggcatca
     7561 gcctctgtcg ttaaatggga tgcaaaaaaa cgtatgaaac gacaacaacg gcttgaaaag
     7621 attgctcaag aggcagcgga acaatcccat cggcagatcg tgccatcagt agagttattg
     7681 gaaacgaaac aagatttatt tgagcggcta gatgattatg acgtggtctt ggttgcttat
     7741 gaggaatcag caaagcaagg cgaaaaatca aatgcagcca aaattcttag tgaaacaaaa
     7801 ccagatagtc gaatcttagc tgtctttgga ccagaaggtg gattatcccg cgatgaagtc
     7861 gattattttg tagaacacaa agggatactt tgtggattag gaccaagaat ccttcgtaca
     7921 gaaacagcac ctctttattt attaagtgct gctagctatc aatgggaatt aatgaatcct
     7981 tctttcagct aacgttcttc attaataaaa gagtatcgac agaaagaatt gaaaaaatag
     8041 tcaacaattt gtataataga aacaattatt tatacaatgg atacgaaaaa tcgtttgaaa
     8101 aagcttttaa aaagcgaaca tttccgtaaa aaaacgtata actgcacgga ttttgggaca
     8161 attatcaaac agatatttgt cccgattccc gttttaccga aaaaagaaag ggggatcgat
     8221 tcctgtgaca aaagaagaaa ttttaacggg acccggtgtc ataaagctcg ttagtcaata
     8281 catgggtccc gaacatgtag catttgttga gaaagcatgc gaatacgcga ctgctgcaca
     8341 tgacggacag tttagaaaat caggcgaacc ctacattatt catcctatcc aagtcgcagg
     8401 tatattagca gatttaaaaa tggatcccca tacagtggct acaggcttct tacatgatgt
     8461 tgttgaagat acagaaatca ctttagaaga tctgagagaa gaatttggcg atgacgttgc
     8521 tatgttagtg gacggcgtaa ccaaattagg gaaaataaaa tataaatccc acgaagagca
     8581 gctagcagaa aaccaccgaa agatgctgct agcaatggct caagatttac gagttatcat
     8641 ggtcaaatta gctgatagac tacataacat gcgtacgttg aagcacctgc gagaagataa
     8701 gcaaagaagg atcgctcagg aaactttaga aatctatgcg cctcttgcac atcgtctagg
     8761 gatcagccgg atcaaatggg aattagaaga tacagcactt cgttatctaa atccaaaaca
     8821 gtattaccgt atcgtccact tgatgcagac gaagagagaa gaacgtgaaa aatacgtaag
     8881 tggtactgtt gaagatattc gaatagcgac agaagagctg gggatttttg cagaaatcta
     8941 tggacggcca aaacacattt attcaatcta tcgtaaaatg aaagatcaga aaaagcagtt
     9001 caacgaaatt tatgacttgc tagctattcg agtgatcgta gattcaatca aggattgtta
     9061 tgctgtatta ggagcaatcc acacaaaatg gaaaccaatg cccggcagat ttaaggatta
     9121 tattgccatg ccaaaagcta atatgtacca atctttgcat actaccgtga tcggtccggc
     9181 aggaaatccg gtagaaattc aaatccgaac acaggaaatg catgaaatcg ctgaattcgg
     9241 ggttgctgca cactgggcct ataaagaagg aaaaaatgaa aaagtagaac cagatggtat
     9301 gacgaaacaa ttaagctggt tccatgagat actcgaactt caagacgaaa gctatgatgc
     9361 ttctgaattt atggaaggcg taaaaggaga tatctttagt gataaagtct acgtcttcac
     9421 accaaaagga gacgttactg agttaccaaa aggatccgga ccattggact ttgcatacag
     9481 tatccataca gatatcggta acaaaaccac tggtgcaaaa gtaaatggca aaatggtgca
     9541 gcttgattac aaattgaaaa acggagatat cattgagatc atgacttctc caaattcatt
     9601 tggcccaagt cgcgactggt tgaaattagt tgctactagc aaagcaagaa ataagatcaa
     9661 acgtttcttc aaagcccaag atcgagaaga aaatgtgatc aaaggccacg aatccgtggt
     9721 caaatgtatt acagatctag gatttacgcc taaagatatt ttgacgaaga acaaactgca
     9781 agaagcactc gatcgtttta attatcaaac agaagatgat ctctatgcag ctgtagggta
     9841 tggagaagtt agccccttga cgatggccaa tcgtctgact gaaaaagaac gtaaagaaca
     9901 aaaaatcgag cagcaaaagc aagaagcaga agaaatcatg aatcagccga aaaaagaacc
     9961 tgacaaaatg aaagtacgtc atgaaggtgg cgttgtcatt caaggggtag aaaacttact
    10021 aattcgtatc agtcgctgct gtaatccgat tcctggtgat gatatcgttg gttatatcac
    10081 taaaggcaga gggatatcca ttcatcgtcg agattgtccg aatgttcagc ctgacaaacc
    10141 aaatgtagca gaacgtttga ttgaagtcga atgggaagat acatcgaata cacgaaaaga
    10201 gtatgatgca gatttggaaa tttacggcta taatcgttca ggcttattga atgatgtact
    10261 tcaaacagtc aatgcgctaa cgaaaaatct caacagcgtt gaagcacgga cgaataaaga
    10321 taaaatggcg acgatccatt tgacggttgg tatccagaat ttatcccacc taaagagtat
    10381 cgtggataaa atcaaagcag tacctgatgt ctacagtgta cgccggacga atggatagga
    10441 ggacagatat gagagcagtt gttcaacggg taagtaaagc tgctgtgtca gtcgatcagc
    10501 aagaaattgg aaaaattggc tacggcctac ttgttttatt aggcattcat gaaaccgata
    10561 cgcaaaaaga tgtggactat ctggtaaaaa aaattgcaca aatgcgtatt tttgaagatg
    10621 aacaaggaaa aatgaatatt agtgtagaag atgtgggagg acaaatttta agtatctctc
    10681 aattcacact gtttgcggat acgaaaaaag gaaatcgacc aagctttgtg gaagccgcac
    10741 gaccagaaac agctattcct ttatacgaat cattcaatga aggaatcaga agtcgcggca
    10801 tcacagtcgc taccggacaa ttcggagcag atatgtcggt ttctctcgtc aatgatggac
    10861 ctgtaacgat cattattgat tctaaaaaca aataaagtaa tctggttttc actagtaaaa
    10921 actatgaaac acgtcgctta agttagactg ataaactcta acttaagtgg cttttttgtc
    10981 tgcaaaaagc gcttcttttg tcagcagtcg gaaaaaaagt taagcttaaa ttatacaagt
    11041 tattttttat aggaggttgc tgaagatgaa agaagtacca gtagtagata tcaaaatcac
    11101 agatttaaaa aaattgtatc aaaaaggata caacatgttg gaggagttac gccatgaagc
    11161 ggacgctccc gtcgtcaaag caaagatttt caataaagaa gctatcacga tctacggatc
    11221 atcagcagca aaagtatttt atgatccaag gaatttcaaa cgaaaaggtg ctatgccaaa
    11281 attagtattg aagacacttt ttggtcaagg tggagtacag actttggatg gagctgctca
    11341 tcaccatcgg aaaaatattt ttatggatct aatgacaccg gaaagaatgg aagattatca
    11401 tcgtatctta gataagaatc tgacacaagc acttgaagca cagcatggtc aatttgaact
    11461 atttgattta agtaagatgg ttttctttac atctatttgc gagtgggcag gtatcaattt
    11521 atcagcgatt tctaaagatg aggtcgaaaa attagcagaa tatcaaatct ctatgatcag
    11581 cggaacgttt acttctccga ttgatcacat aaaaggtgtc gaaaatcgca aaaaatcaga
    11641 aaaatgggcg caaggcttga ttgaagaagc tcgacaaaat cctgtggctg gaaaagaaaa
    11701 tgtggcatta tatgcttttg ccaatgccac agatttagat ggtcagcttt tacctctgga
    11761 agtggcagct gttgaattat tgaacatcat ccgtccaacg gttgctctaa ctgtgtgggc
    11821 ggcattgatg ggacatgcac ttttcagtag accagatttg tatcagcaat taaaaaatga
    11881 cttttcgacg ttacaagatc cgtttattca agaaatgaga cgctattatc ctttcttccc
    11941 aatgctacct gcgatttcgc tgaaagaagt agaagtagat ggttatcgta taccagaagg
    12001 cagttgggtt attttagatt tatatggaac agaccatgat gaacgaacag ttgaagcgcc
    12061 agattccttt atgatcaaac ggtatgtagg gaaagcaaaa gatatttcct ataaagaaga
    12121 atatgagatg attgcacaag gcgggggaaa ttttcgacaa atgcatcgct gtgcaggaga
    12181 atggatcact cttcatagtc tacgtgtgtt ttccgatcag ctagtaaata agtttgagtt
    12241 ctctgtacct gaacaagatt ggacgattcc attcaatcag tttccgactt atccgaatag
    12301 ccgagcgcta ctttacaaga attagattag cataaactta tcgaaaaaaa ggaagatcaa
    12361 acagtgctga tcttcctttt ttcatattac tataaattga taaggtcaat tgatcaaaat
    12421 ccacgaaatg cttcatcaat ggtttgataa tcttcattgg ataaatgcaa atctaaagct
    12481 tggacattgt cagatacttg ctctggacgc ttagctccag gtataactac agacactcgt
    12541 ggattcttca tgtaccaagc taaaacaagt tgcgcaggag tggcttggta tcttttagca
    12601 atggggcgaa gtacatcgac agcggttaaa atttctctga aacgttctcc ttggaaatct
    12661 ggatttcttt ttcgaggatc tccttcacca aattgttttt cctcaccaag ttcatatttt
    12721 cctgtcagca aaccggaagc aagaggaaaa tagggaacaa aactgatttt gtttttttca
    12781 agataaggga ataattcttt ttcagcctgc cgatggatca aactgtattt atcttctact
    12841 acatcgacat atccatctgc attcgcttct ttcaattgtt ctaacgtgaa gtttgacaca
    12901 ccgactgctc ggatttttcc tgcttctttt aattcgtgca atgtagcaac tgattcattt
    12961 tttggtgtgc tttcatccgg aaagtggata taaaaaatgt cgatgtaatc agtttgcaga
    13021 cgttttagtg catcttccac cgattgtttg agaaactcag gagaattatc aaatgttcgc
    13081 cctttatttg gtacatgagc agcttttgta gcgatgacga cacgtgagcg atcatattct
    13141 ttttccttca gtacctcgcc aatcagttct tcggaacggc cgtttccgta agcataagcg
    13201 gtatcgatca aattgatccc gctattcaaa gctgtacgaa ctacttgttt tcctgtctca
    13261 tcttccaaac cgtcaaaaag attatgtcct ccaacggcat ttgctcctaa tccaagctgt
    13321 tctgcgtaga cttgtgaatg tccgattctt atttctgcca tagctgattc ctcctttttg
    13381 tctagtctaa aacaaaatac caaaaagctg aactaaaacg tttttgtgag gggaaatagt
    13441 cagatatctc ttgactttga gactggtttt tagtaggtta taagtaatat cgaatgtcaa
    13501 tgaaagagcg agtaagtttt tgaaacttta caagagagga tcatcgaggc tgaaaatgat
    13561 ctaaagtttc attagcctaa agacactctg gagatggacg agtgaaaagc cgtcccgcgt
    13621 acgagcgtta ttcgtaagag gaaagagaaa tctttcaaaa ttaggtggta ccgtgtatga
    13681 atttcattca cccttgttca tgatgaacaa gggtgatttt tttatttaca tagctaagaa
    13741 agggagagaa aactatgagt ttccaaagac caaaaggaac aaatgatatt ttgccaggcg
    13801 aatcagaaaa atggcagttt gtcgaagaaa cagcacgttt attattcaga gattatcagt
    13861 ataatgaaat gcgtacaccg atttttgaac actatgaagt gatatcccga agtgtagggg
    13921 atacaacgga tatcgtttca aaagagatgt atgattttta tgacaaaggg gaacgccatg
    13981 tgacgcttcg accagaaggt acagcaccaa tcgttcgttc ttttgttgag cataaattgt
    14041 tcggcccaga gtatgcgaaa ccgttcaaaa ctttttatat gggaccaatg tttcgctacg
    14101 aacgccctca aaaaggacgg ttacgtcaat tccatcaaat cggagtagaa gcatttggat
    14161 cagaaaaccc tgcaactgat gtggagagca tggtgatggc acttgatttc ttcaaacagt
    14221 tggggatcac gcaaatccgg ctagtcatca attcattagg tgataaagaa acgcgtcaag
    14281 cctatcgtca agcattgatc gactatcttt taccttttga atcagagctg agtgaagact
    14341 ctaaacgccg ccttcatgaa aatccattac gcgtattgga cagtaaagac aaacgagatc
    14401 aaaaatttgt ggcagaagct ccatctatct tagattatct aagcgagcct gctcaaaaac
    14461 attttgatac cgttgtttcc atgctggatg cattggctgt tccttatgaa atcgacagta
    14521 atatggttcg cggactagat tattacacac atacgatttt tgaaatcatg agtgacgcac
    14581 caaaaatggg ggcacaagca acgatttgcg caggcggacg ttatgacaat ctagtggaag
    14641 aactaggtgg tccttcaaca cctggcttcg gatttgcaat gggtatcgag cgattactct
    14701 tgactatgga ggcagaggaa gtagttatcc ctgcatttaa tgagttggat gcttacgtgg
    14761 tcgcactcgg agacgaaaca aatatcgaag cattgaaagt ggtccaagcg attcgaaact
    14821 ttggtttttc agctgaccgc gacttcatga accgtaaagc aaaagcgcaa tttaagacag
    14881 cagataaagc gaatgccaaa ttagtattgg tgatcggtga agatgagctt gctagtcaaa
    14941 cgattaatgt caaagcaatg gcaagccgaa aagaaaaaag ttttccatta gaagagatat
    15001 atgaacattt tgatgaagtc tatgatgaaa tgactttatt cgagtaggag gaaaaaaata
    15061 aatggcacaa agaacgatct attgcggatt agtttccgaa caacaattag aacaaacagt
    15121 tacattaaaa ggatgggtac aaaaaagacg agaccttggg ggagtaatct ttatcgacct
    15181 acgtgatcgt gaaggaatcg ttcaaatcgt ctttaatcct gaaatcaaca aagaagcatg
    15241 ggaaatcgcg gataaatgtc gcagtgaata cgtgatcgaa gtgactgggg aagttagaag
    15301 acgcggagaa aatgcagtaa atcctaaaat ggccactggt gaattcgaag tgattgcaag
    15361 tgaaatcgtg attttgaatc aagcaaaaac cccgccattt caaattgaag atgatcaagc
    15421 aatcagtgac gaaatacgtt tgaaatatcg ttatttggat ctgcgtcgtc caaaaatgac
    15481 taaaaacatc aaattaagaa atgcgacaac aaaagcgatt cgtcattatt tagatgataa
    15541 tgactttctc gatatcgaaa caccttacct agggaaatct acaccagaag gcgcaagaga
    15601 ctacctagtt ccttcacgtg tccatgctgg acatttctat gcgttgccgc aatctcctca
    15661 attgtttaaa caattgctga tgaatgctgg attcgaccgt tattatcaaa tcgttcgttg
    15721 tttccgcgat gaagatttac gtggagaccg tcagcctgaa tttacgcaag tagatattga
    15781 aacaacgttc ttaacagcag aagaaatcca aacttacaca gagggattga ttgcgaaggt
    15841 aatgaaagaa gttcgcggta tcgaagtcac attgccattt cctcgtatga cctatgatga
    15901 agcaatggct cgctacggca gcgacaaacc ggatacacgt tttgatatgg aattgatcga
    15961 tctaagcgat acagtcaaag aagtagaatt caaagtcttc caaatggctc ttgaaaacgg
    16021 cggtgtcgtg aaagcattga atgctaaagg cgctgcagat cgctactcac gaaaagacat
    16081 ggatcagtta ggtcaatatg taggacaatt cggagctaag gggcttgcat ggctgaaagt
    16141 agaagaagac ggattgaaag gtccaattgc taaattcatg ggagaagcaa cagaagcaat
    16201 cattaaagca acagatgcaa aaccaggtga cttgctgatg tttggcgcag ataaaagtga
    16261 aatcgtcgct gctgcattag gtgctatccg gacacgttta ggaaaagaat taggactaat
    16321 tgatgaatcg aaattcaatt tcttatgggt aactgattgg ccacaatttg aattttcaga
    16381 agaagaaggt cgatatgtct ctgctcacca tccattcacg atgccaaaag aagaagatat
    16441 cccgttattg gctgaagacc ctgcaaaagt ctatgcagaa gcttatgata tcgtcttgaa
    16501 cggttatgaa ctaggcggag gttctatccg tattcatact cgtgagcttc aagaaaaagt
    16561 actagaaact ctaggcttta caaaagaaca aatggaagaa caatttggtt tcttgttaac
    16621 agctttagat tacggattcc ctccacatgg cggtattgca ttaggattgg accgttttgc
    16681 aatgttatta gccggagaag ataatattcg tgaagtcatt gcctttccta aaaacggaaa
    16741 agcagcagat gtgatgagtg atgcaccaag tatggtttcg cctttacaat tgttcgaatt
    16801 gaatattgat gtaacgaata tagaagaata gccgataaaa aggtaaaaag gatttgtcga
    16861 atacgcttct tttgacaacc ttgatttaat tgaaaaacac tttataatga agccataaca
    16921 tataaagcgg ggcttttcca tgaaaaaaac attcgaaaat tgggcttatc atgattacac
    16981 gaccaagctt tcggtatcta tcgtttatgc gttgatcgca tcagtcgcat tgaatttctt
    17041 ttatcagcca ggacatattt attcaagtgg tattactggt ctagctcaga ttttgacgac
    17101 tttgtcaaaa tcactgatag gattcgaagt accggtatca acaactttgt atttactgaa
    17161 tataccttta tttgtattgg cgtggttaaa aatcgggaag aagtttacaa ttttcacttt
    17221 tatcacagtc actttgacct ccctttgtat ccatatttta ccggaaacag tcctatctac
    17281 cgatccaatc atctgtgcgt tgtttggggg ggctgtgatg ggactaggta ttgggctagc
    17341 gttgagaaac ggcctatcat cgggcggtct agatatcgtg agtatcacga ttcgtaaaaa
    17401 aacaggaaag tcgattggtt ctatttcgat ctacttcaat gcattgatcg tttttgttgc
    17461 cggaattcta tttggctggc aatatatgtt ttacagtacg ttgtctattt tcgtcagtgg
    17521 aaaagtaact gatgtcgttt atactaaaca aaagaagatg caggtaatga tcgtaacgaa
    17581 acatccagaa aaagtagtaa atgaaatcca aaactgtatg cgtagaggga tcacgatcat
    17641 caatgaagca gaaggtgctt ataagcatga taagcagaca gtattgatga cgatcgttac
    17701 acgatacgaa ctgcctctac ttagagaagc aatgaaagct tctgatccac aagcttttgt
    17761 aagtattgca gaaaacgtcc agatacttgg tcgtttctat gaggatggca tttagatcga
    17821 gtttgacaaa aattcatgaa atactgctgt agacagacgt tcgtttgtct gcagtttttt
    17881 tctaccatag tagaatcagc aaagaaaaac tgctttttta tttgaaattt tcttaaagtt
    17941 gaaaaaaagc atgtttattt ttttaaattt atgggatgat agaaaaaaat agtagagaaa
    18001 ggacacgatt ctacttgcta ttttcactta atacccgtat attacgaatg aaaaaagttt
    18061 atgaaaagtt ggaatattga atggaaaaac aaaaaaggtt aaaaaacagc cgctacatca
    18121 ctgggtttga tgggctccgt tcattagctg tggtgggtgt tatcttctat caccttctgc
    18181 caactagtat gaaaggcggc tatttgggcg ttccgatttt tttcgtcgta tctggttatt
    18241 tgatcacgga tctgctgcgg caggaatggg aacaaaatgg aaaaatcaat atttggcaat
    18301 tttatgtcag aagaatgaag agactctatc cggcacttgt ttttatgctt gtgacagctt
    18361 cggcttatat cactttgttt cagcgagggc tgttaaacaa tcttcgagga gttgtggtca
    18421 gcagcttact gtatgtcaac aactggtggc aaatcaataa cggtttatct tatttcgatc
    18481 gatttgctaa tgagtcaccg tttacgcata tctggtcttt agcagtagaa ggtcaaaact
    18541 atttggtttg gccgatcttg tttgtattgt tgatggtatt tgttcgtaaa agaaaatgga
    18601 tcttttatac tgtgttaggt acatccttcc tctccgccat cttgatgatg atcttgtata
    18661 caccaggagg cgatccaacc cgtgtttatt acggtaccga cactcgtctg ttttctatat
    18721 ggttgggaag tgcgcttgcg tttgtttggc caagtacgcg tttaaagaaa aacatcccga
    18781 aacaagcgaa aaatattctg aatatcgctg gaggaatctc gcttttggca ctgattcttt
    18841 gcttcttctt tttagatgat cattacaatt tcatctatta cggaggaatg ctcctagtga
    18901 gtctgttatg cgtagtgcta gttgcagtga cagcacaccc cggagcaagt ttgaatcgat
    18961 ggctgacaaa tccagtattt acttggattg gcaaacgaag ctatggcatt tatctgtatc
    19021 agtttccagt tatgattttt tatgaagcaa aggtgactga tattgccgat catgttttgt
    19081 tgcatacttt agtggaaatc acattgattc taatcatcag tgaactttct tatcgatatg
    19141 tggagcgtcc gctagcacgt tttgattatg gtcgtacatt ccaagtgatg aaaggatggt
    19201 tcagcaaacc gatcatcagt aagagcaaac catgggttgt tccaggcaca ttacttgtag
    19261 caattgcact cgttggtttc gttacggcac cgaaaaacgc tgtaacagcc gatcaaaaac
    19321 aactgcaggc aaaaatcgct gaaagtaaaa aattagcaga agaaaccaga aacaattcag
    19381 aaaacaccac tagttcggtt gaccaagcga tccttgataa gtacgagttg tccaaggcac
    19441 aagggaataa ggcacaaaaa ttagaactaa cggcatttgg tgattcagtt atgttggatg
    19501 ctgcaacgga tctacaagaa ctttatccaa aagtagtggt agatggagat gtcggacgac
    19561 aattatatgc cagtgtaccg tacattgaaa aattaaagga acaagatttg ttgaaagaca
    19621 ctgttctgat cggactagga acgaatggct cattctcgga agctcagttt gatagtgtga
    19681 tggatgcaat cggtgatcga aaagtatact gggtcaatgt ccgagtaccg acgaagcgct
    19741 ggcaaaatga cgtgaatgcg atgcttgaac agatgacgaa aaaatacgac aatatgacgc
    19801 tgattgattg gtacagctac agtaatgacc atagtgactg gttttatgat gatcaagtcc
    19861 atccaaatcc tgaagggatg acacattaca ttcacttagt cagcgaagcc ttgctaggag
    19921 gaagttcaag tacgaacagc agtgagacaa acgtgacaga atgatagcta caaaaaactt
    19981 caggcaaaaa catttgtttt tgcctgaagt ttttttatat ttaccgtgtt tttaacattc
    20041 atatagattc aggaagcaaa acggttgagt gtgtctgtaa ataacgttct ttttgttttc
    20101 gggtgagttt tttgaatgca gcttctgctt ttgttgcttc gcttcttgaa gaaaatactt
    20161 ctgcatggat catcttaaca ggtcttcttt tttgaagacg agtatattta gcaccagtac
    20221 cattgttatg ttcctctaat cgtctagaaa gatcggttgt atatccacca taaaatgtct
    20281 gatctttaca gaataatacg taaaaataat gttcatttgc catagagcat ctcacgaatc
    20341 tctgcagtgt attctccatc catattgtaa gtgatcaatg gtggagcgac tttgaatccg
    20401 tctaatttgc cttctttgat tccttcaact aacaaaatat ttgcctcttt tccaggtttt
    20461 gggtaaacaa attggatcct tttaggtgca attcgatagt cttccatgac ctgaatgatt
    20521 tctaagaacc gatcaggacg atggaccata gtaaagcgtc catttgtttt tagtagctta
    20581 cttgaaatat cgatcacttc ttctaacgtc gtatgaatct catgacgggc gattgccagg
    20641 tgtttattag ggttcgtttt atttgttggc agccctttga aataaggtgg attacagacg
    20701 actaagtcac aagaattatg tcgaacgaca gataagctgt ctttcagatc gatggtgtgg
    20761 acagtcattt gttcatttaa atggttaagc gcgatactac gtcttgccat gtctgctaga
    20821 cgaggttgaa gttcgatagc gtcgatagcc gcctgggttc gtttacttaa aaaaagtcca
    20881 actgcgccat ttcctgcaca aaggtcaaca atcttccctt ttttaggtac acgggaaaag
    20941 tgagcaagca gcacagcatc taaagaaaaa gaaaacactt tggaactttg gataatcttg
    21001 atatcttctg cgtataattg gtcgattcgt tcgtcttcgt gaagcatgga ttccctccaa
    21061 aatgaaaaaa atagtcgtct gtattatact atttttatta aaaagaacca aggtggcttg
    21121 tcaaacctct attttttatg catactatga ccataagaag ggaaaggaag agactatgtt
    21181 ttatcgattt atgcgcgggg tagttcgagt actattagcc ttagttaatg gaaatgcccg
    21241 ctatgagaat aaagaagttt taccgcaaga tgagaattac atactagttg ctccccatcg
    21301 tacttggtgg gacccattat atctggcggt agctgcagca cccaaggaat tcagctttat
    21361 ggcaaaagaa gaactgttca aaaatccagt tctgcgattt atattaactc gatccaatgc
    21421 ttttccagtt aaaagagaaa atccaggacc aagcgtcatt aaaacacctg taaagatttt
    21481 gaaatcgaca aatttgagtt tgatcatgtt tcccagtggg acaagacatg ctacagaact
    21541 taaaggcgga atggcattga tttccagaat gtctaaagcg aaaatcgtgc cagctgtata
    21601 tcaaggtccg ttgacacttg gggatctgtt caagcgtcag agagtgacag tgcgtttcgg
    21661 cgaaccaatc gacttatcag atatcaagaa aacggataaa gaaggtatgg cggagatcga
    21721 acgacgaaca caagcagctt ttgacaaatt ggacaaagaa gtgaaccctg atttcaaata
    21781 tgaagtcaaa aaaaaataaa atgataagtt tatgcgtggg cggtcttttg aatctactgt
    21841 cctgttcagt tcagactttt tcaatgaaaa cgagggagag acgtcaagtc tgccctcttt
    21901 ttgcgttcca atatgtaaaa tagaagacag gtgtcatgta atggatcaaa gtgttataat
    21961 aattagaagg acattttttg aggtgattga atgcgttttt tacatacagc cgattggcat
    22021 atcggaaaaa aactacaagg ttatgacttg attgaagacc agcgtcatgt actatcttct
    22081 atactagata tcgcgaaaga agaaaaagta gaggcaatcg tgattgccgg agacttatat
    22141 gataggagtg tcccagctgt tgatgccgta gaattataca atgaattgat ggtcgattgg
    22201 aatttaaagg aaaagatccc gatttttgct atatcaggaa atcacgatag cagtacgaga
    22261 ctttcagcgg gtacgccatg gttcagttat tcgaactatt ttttgcgaac acagctagca
    22321 gaagcatttg aaccagtcga gtttaaaaac gtacaatttt tctttcttcc ttactttgaa
    22381 ccaattgcag cgagattata ttttgaagat gacaatatcc gaacgatcaa gcaagcggta
    22441 gccaaggtga tagataaaat gcaggagcta tttgattcag aaaaaaaaca agtattgatt
    22501 agccattttt ttgtaatggg aagtgtaaaa tctgattcgg aaacgaaaat cgaagtaggc
    22561 gggttggatt cgatccctgg tgaactactt acaccgtttg attatgtagc actaggacat
    22621 ttgcataatc aagctgcttt gagacaggat aatgctcgct atagcggttc cccattgaaa
    22681 ttctctttat ctgagatcaa ccaaaaaaaa ggcgtatgga tcgttgacct gacaacaggc
    22741 ctggagttga attttaggga gatcgaaccc ctgcatgata ttgaagaaat aacagcgagt
    22801 ttcaaagaat tgctggatcc tgccttttat cagtccgtca atcgggagaa ttacatctat
    22861 atctatctca ctgatagaac gatcatcccg aatatgatga accagcttcg gcaaatatat
    22921 cctcgtattt tggggattga acgggcaaac ggtcgagcag aacaaacaaa gcgcaaacga
    22981 aaagtactag aaatgaaaga tcctcaaaaa ttagcagaag cattttttag tgaaatgacg
    23041 gaagagcagc taactgcaaa ccaagaaaaa tggctcggag aaacgttgca agaaatcatg
    23101 gaaatggaat gatcaagaat gaaaccgaaa aaattacaac ttaaaaattt tggtcctttt
    23161 atcaatgaga ccgtagattt ttcacgtttg acagaagcac cgcttttttt gatcagcggg
    23221 aagacaggag ctggcaaaac gacgattttt gatgggatca ccttcgcact ttttggtgaa
    23281 acttccggaa gacttcgctc tggaaaagag atgcgttctt tgtttgctac accagacgaa
    23341 gaaacaagcg tgactttttc ttttgaacat cagcagatga gctatgaaat cgaacgaaaa
    23401 ccagaacaag ttttagcaaa acgaaaagga aacggaacaa aaaaacaggc agccaaagta
    23461 acgttgacca tttttgatgg gaaaggaaaa gaaataaaac aaatcacaaa acgtacagaa
    23521 gcagatcagt tgatcagaga actgatgaat ctcgatgcga aacagttttc tcaaatcgtt
    23581 ttactacccc aaggagaatt ccgtaatttt ttggtttcat caagcagtga taaagagact
    23641 gttttacgtc atttattcgg tactcaattt ttccaacagt tcaatgagcg attaaaagaa
    23701 aaagcaaaac aacagcaaca aaaattggat catttagagc aagagctgca tcttctgcaa
    23761 aaaaggtttg ttttagtcaa agaagaggaa ccagcagcag ggtttgaagc cgttttagcc
    23821 caatggacaa accatcaggc aatactcgaa aaacaaatca cggaagaaaa agaagctcta
    23881 acggaacttc aggaaaaaca aaagcaacta gaaaattcct actatcgttt tgaaagcttg
    23941 aaaaaaagtt atgaagaaaa aacgatatta ataaacaagc aaaaggaatt agctgctcaa
    24001 aaagacgaaa tagaggaaaa gaaacgatgg attgggtatt atgaatttac gcaacgatta
    24061 gtcgaaccaa tccagcgcct acaagaaata aaagttgaaa aaatagaact gtcacaaaaa
    24121 agtagtgagc aaacaaagaa actagcgatg actacgcaga attatgaaga gtggcaaaaa
    24181 aatgaagcaa accgcgtacg ccgacaaaca aaaatcaaag aagctgctca aaaactgcaa
    24241 aacaaacaga tgatggtgcc aattgtcgaa tccttacgta gtaaaaaaca agacgcagaa
    24301 caattaacaa tttcattgga taaagaagaa ttgaatgccg agaagctgta tcaaaatctc
    24361 gctatctatc aggaaagaac tcgaattctt caagaaaatt tactggatca ggaaaaactg
    24421 caagaagatc gtcttgcttt tggacagtta aaatatctag atgaaaagca gcaagaaata
    24481 gaaaaacaaa aagcaaaaga cactgggtcg ctcgcacaga taagagaaga actcaaaaaa
    24541 gcaactgaag aaataacaaa aaatagtcag ctattgaaag aacagacaga acaagcagaa
    24601 cagctgaaaa gccaatgggc aaagcagcaa attgctcgtc ttcagctttt tttgcgtcca
    24661 ggggagccat gtcttgtttg tggatcaacg gaacacccca tacaacaatt aaaacacgaa
    24721 cagccaaata aggaagatat cttaaaagta gaacaacaac tggcagaagc tgagtctgct
    24781 gtagaaagaa ctcagcaaat cattgccaaa agtgaagcac atgaaaataa tcttcaacaa
    24841 caattatcag atttagaaca aaaaatcatg gcaacgaata tacaattggc tgaacaagaa
    24901 gaacacttgc aacaggcttt gaaaaaggca tttcctgaga taacagaaca aaaaacaaaa
    24961 acaactcttg tagtaatcga acaatcttta gatagacgaa aaagcaagat ggccaatgca
    25021 agaatagagc tggaagaaat aagacaacaa gcagaaaagc tcgaacaaca attagctgtc
    25081 tacaaacagc agcagacaga aaaaaataat cagctccagc aactgcaagg agaaatcaaa
    25141 agtttagaac aacaattgca agatacggat agtaactgtt tagaagagga gatacgacga
    25201 cttgaaaaac agttagaaga agactcgaat caactagctg ttgagcagaa agcaggagtg
    25261 caattgttgc aagatcttca tacgcttcgg acacagcttg atcttcaaca tgaacaaata
    25321 gaaaaaatag atgaaaaaat agaaaaggaa caagaaagaa tagctaaaaa gttagaaagc
    25381 cagtcgtatt tttcaacaat tgaagaaatc tgtgcatttg cacaacagca agcacaatat
    25441 aaagaaaacc aacgtacagt tacttcttat catgaagagc aattagtgac agctgaccgg
    25501 ctgcgtcaat tagaaaatat ccagctaact gagacattcc cagatacaga agagatcagc
    25561 caacagttag aagaaataag acgtatgatc gaaataaagc aagaaaagat atacggttta
    25621 caagaacaaa aaattagtaa tcaaaaaatc tacgatgaat gtctttcgat ttatcacaca
    25681 agccagacga aattagatga gcttacgcaa ttacagcagc tttcgcaaac attcaacgga
    25741 gaaaatccta agaagactag tttggaacgg tatgtgcttc aggtttatct tcaagaagtg
    25801 ttacaagtag caaatgatca tctccaacgt ttaacgaaaa atcgttatca atttgagcta
    25861 gcggatacca ttggcagtta tcgagggaaa accggattag aaatcaatat ctatgatgat
    25921 caggcaggta tgtctcgaag tgcgcatacc ctgtctggcg gtgagagttt tattgcagca
    25981 ctttctttag cattagctct tgccgatgtg atccagacac aagctggtgg tgtagcaatc
    26041 gatgcattgt ttattgatga aggatttggg tcattagatg aagaagcttt ggaaatggca
    26101 atggaagctt tagaaacgat cgaaagtgaa ggaagaatga tcggcatcat tagtcatgta
    26161 agagaattga aagaacgagt attacaacaa ataagaattg atacagaagg tagcggacaa
    26221 agcaaaatac gctatcaact tggataaaaa gggagattgg taactatgaa atggagtatt
    26281 ccagaaaaag tcattgcacg tggacgtcaa tacttgcaag aaggccgagt gttatcggtt
    26341 gtccctgacc aagaagaaaa cgtgtggcat gcagaagtct tgggcagtga attgtatatg
    26401 gttgatttgg atggtacagc aaaagagata gattactgcc aatgtcccta ttgggaggaa
    26461 cataaatatt gtaagcatac agttgctgtg gaactttatt tacggtcgaa aaatctatct
    26521 cgtatcatga aaaaagacca gccaatgcta gaaccggtaa aagcaacaag tgagggagaa
    26581 atcattgcaa aaggtttttc tcgtctaaaa acacaaaaca atcctcaagc ggtgcaacct
    26641 ttgattattg aataccaagt agaaacgatt gagactaatc agtatcaccc agagctttct
    26701 attttggcac tttacttgcg tatcggttca ttggcatcac cgaaaaaaac atatatcgta
    26761 aaaaacatct atgatttttt acaagcttac acagaaaaag aaacttacac tgttaataaa
    26821 cagtaccagt ttcgtctaga taaacgggcg tttcaaaaag aagatcaaaa aatactcacg
    26881 catcttgcag gaagtgctca gacgcatcaa ttattaggta cgaacggctt acaagtcaaa
    26941 ggaaaactag ataaaagata tctgcttctc ccagtagaac aagcccgctt cctgttagag
    27001 cagatgaatc agactacacg tttgcatctg cagataaatg aacaaaacga tcaaggaata
    27061 caattttccg atcaaaacaa acctttatca ttcaaagtgg aaaaatttgg agaagattat
    27121 ttgctgcaag cattgaatga ttttgatttt ttctttatgc attatcagtg gggagttatc
    27181 caagggacat tttattctct gacgaaatat caacaaacgg tttatcaaac ttggaaacaa
    27241 ttgattcgcc gattggagaa accagaagta gtgttcaaga aaaaagaatt accgtctttg
    27301 tttaaagaga ttatccctct gctgtcagag atcggcgacg tagctattgc ccctgcagta
    27361 gaagaggaag tagcagatat ccccgtcgaa tttattttct ttttccgaaa agcaaaagga
    27421 aaaatccaag ctcgagtcga tttcgtctat ggcgaagtca tctattcgac tgacccgaag
    27481 catgaggtct cgacagaaca ggcttttcag gtgttacgtg acacagtaga agagaaacga
    27541 gtacttgatc tattcagaat gtatcgctac cagaaaaatg aaactggtta tgaacgtcca
    27601 ttgccaagcg gggaagaact atatgccttt ttccgcacag aactatctgt cttccgtcag
    27661 tttggtgaag tcagacttgg aagaaaattg cgagagttgt atttagatgc gcataaacat
    27721 caaccgaaaa tcgaagtaac cgagaccggt tcatggctcg atattcgatt cgatgtgaca
    27781 gggatagagg agcaagaaat cgatcatgtc ctgcaaagct tgctgagaaa tgatgctttt
    27841 tatacattag aaaatggtca ggttctttct tttgattcag aagaatttca acaaacaagc
    27901 caagtgttgc aacaattacg ggaatcgata cgtacagaag aaggaacgat ccatgtacct
    27961 aaaaatcaag gattgatcat tcaaaatcaa ttggaaaaaa gcaatgctac ttttagtgaa
    28021 tcgttccaaa cgatggtaca agatctcatt catccagaaa gataccaggc acagcttccc
    28081 aaaggattga atgcaacgat gagagattat caaaagcaag gttttcgatg gttgaagatg
    28141 ttaggtcatt atcagtttgg cggtatttta gccgatgaaa tgggacttgg gaaaacacta
    28201 cagacaattg cctttttact ttcggaaaaa gaagaacgga aaagcttctc tgctttgatc
    28261 gtagcgcctg cgagcttaat ttacaactgg caggcagagg tgagaaaatt cgccccttct
    28321 ttaagtatcc aagtaatcaa tgggaataaa aaagaacgtg aagaattact ggctaaagac
    28381 acagatatcc gagtgacttc ttatgccagc ttaagacagg atctagctaa ttatcagtcg
    28441 caaaaaattg attatttgat tcttgatgag gcacaaatgg tcaaaaatag cagtacgaaa
    28501 actgcacaag cattgagaga attagctgta cctcagcggt ttgcactgag tgggacacca
    28561 atcgagaata atttagaaga attgtggtca ttgtttgcaa cgattatgcc aggatttttc
    28621 ccgactaaaa caaaattcag ggaacttgcc gcagaagaaa ttgcgcaaat gattcgaccg
    28681 tttattttac ggcgagataa acaaactgtg ttgaaagact tgccggaaaa aacagagatg
    28741 aatctatatt ctgcacttac agaagaacaa aaaactgttt atttagctta cttgcgtcaa
    28801 atgcgtgaag aaatcacatc aatggattct gaagcattca agaaaaaccg gatcgggata
    28861 cttgctggtc tgacaagact gcgacaaatt tgctgcgatc ctcgtctgtt tattgaagat
    28921 taccaaggcg gttccggcaa gctcgaacaa gtaaaagatc tactggtagc tgccaaagag
    28981 aacaaacgaa gagttttgct gttttctcaa tttacaggga tgctgactat cttgcaagaa
    29041 gagttagccg agctagggat ctcgacattt tatttaagag gcagtacgaa accacaagat
    29101 cggttaagta tggtagacgc atttaatgct ggcgaaaaag atgtttttct gatttcctta
    29161 aaggctgggg gaactggatt gaatctcacg ggagcagata cggtcatctt gtatgacctt
    29221 tggtggaatc ctgccattga agaacaagct gctggacgtg cacatcggat cggacaaaaa
    29281 aatgtggtag aagtgtggcg tatgatctct gaaggaacga ttgaggaacg catggattca
    29341 ctccagcaag aaaaacgcga actgttccaa aaagtgatcc aaggtaatga agaacagttg
    29401 acgaaactga ctgaagaaga catccgcatg attttaagtg taggagaaat cgaggagtaa
    29461 gagactcgaa aaagcgttca gtaatgggta agaaagcctt tctaaagaag cgattgttta
    29521 tttgatttaa aaaatagcaa ggaggctggg attccatttt atcccagctt ccttgctatc
    29581 atttttttat ttctattttt aaaggttata caaagattgt cttcattgtc tctaacagct
    29641 gatcaaaaga agaaatctcg atatcgcaaa ttggagtctg ttcaattttg cgttctcgat
    29701 ggttgaacca aagcgcttgc cagttagcac tttttgcacc tagaacatca ttgtcgtaat
    29761 tatcgccaac gtataaggtc tcttctggaa gcatatgaaa ttctttctca gctagctgaa
    29821 aaatctcttt ttctggtttt tggaaacctg ttgcttgaga aatgagcata ttgcgggaag
    29881 gaatccaatt gttcaattga agttgcttca gtttttttgt ctgatggtca gtcggcccat
    29941 ttgtgataat tcctaatggg acattctttt ccttcaagaa atcgagggtt tttttgacct
    30001 cctcatgcaa gcagatatta tcaagttctt cctcgtaaat tttttgaaat aagaggccat
    30061 cttcttccgt aatatgaggg tagtctaaat cttttagtga ttggctgatc cgatgtgctc
    30121 gcatatattc tagtgtccaa tctcctgcca ttacttttgg gaagttttca tcactatgat
    30181 ggcggaaccg gatatatagt ggatgcatat ctgcatctga aacaagcgga acgactcttt
    30241 tgaccgcgtt tcgaaatggc tgctgctgat catacatcgt atcatcaaca tcaaacacaa
    30301 ctgatttaat caaaatttta cacgcctttc ggttttcatt gattttttca caaatccttg
    30361 actattgtaa cagaaaaaat tgtctaaaaa agctgataaa taaaggtttt tcttactata
    30421 tttctatttc cttgacagaa taaggttttt atatttcagt ttttttcact aaaaatcgtg
    30481 aaacacgtca ataagtttga tgaacaaatt aatcattatg gtagaagtcg attgatttat
    30541 tttaatgcaa tctctgtgga attttcgaaa gatttatttt tttaatacag tctcaatata
    30601 actattcaaa cataaaataa cgttatttga tttatttgtt tatagtaaat tttatttagt
    30661 aatcattgct ttaaaatact acgcttatat aacagagaaa ataagcgtta taataaagaa
    30721 cagtattatt taaaatgaca gaaaacatta ctattaaaga aataaaaaac ata
//
LOCUS       DASJTC010000025        29435 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.025, whole genome shotgun sequence.
ACCESSION   DASJTC010000025 DASJTC010000000
VERSION     DASJTC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.025"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(58..624)
                     /locus_tag="U4B19_001010"
     CDS             complement(58..624)
                     /locus_tag="U4B19_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2076767.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(652..2154)
                     /gene="eat(A)"
                     /locus_tag="U4B19_001011"
     CDS             complement(652..2154)
                     /gene="eat(A)"
                     /locus_tag="U4B19_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN2076768.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2412..3533)
                     /locus_tag="U4B19_001012"
     CDS             complement(2412..3533)
                     /locus_tag="U4B19_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN2076769.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3659..4600
                     /locus_tag="U4B19_001013"
     CDS             3659..4600
                     /locus_tag="U4B19_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN2076770.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4750..5646)
                     /locus_tag="U4B19_001014"
     CDS             complement(4750..5646)
                     /locus_tag="U4B19_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN2076771.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5915..7255
                     /locus_tag="U4B19_001015"
     CDS             5915..7255
                     /locus_tag="U4B19_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2076772.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7473..7916
                     /locus_tag="U4B19_001016"
     CDS             7473..7916
                     /locus_tag="U4B19_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN2076773.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8420..9838)
                     /gene="nhaC"
                     /locus_tag="U4B19_001017"
     CDS             complement(8420..9838)
                     /gene="nhaC"
                     /locus_tag="U4B19_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN2076774.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10241..11464)
                     /locus_tag="U4B19_001018"
     CDS             complement(10241..11464)
                     /locus_tag="U4B19_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEN2076775.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11495..12334)
                     /locus_tag="U4B19_001019"
     CDS             complement(11495..12334)
                     /locus_tag="U4B19_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN2076776.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12351..13031)
                     /locus_tag="U4B19_001020"
     CDS             complement(12351..13031)
                     /locus_tag="U4B19_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2076777.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13033..14106)
                     /locus_tag="U4B19_001021"
     CDS             complement(13033..14106)
                     /locus_tag="U4B19_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2076778.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14162..14400)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14443..14676)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14753..15424)
                     /locus_tag="U4B19_001022"
     CDS             complement(14753..15424)
                     /locus_tag="U4B19_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2076779.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15511..18189)
                     /gene="mgtA"
                     /locus_tag="U4B19_001023"
     CDS             complement(15511..18189)
                     /gene="mgtA"
                     /locus_tag="U4B19_001023"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEN2076780.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            18848..19285
                     /gene="ndk"
                     /locus_tag="U4B19_001024"
     CDS             18848..19285
                     /gene="ndk"
                     /locus_tag="U4B19_001024"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEN2076781.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19307..20173)
                     /locus_tag="U4B19_001025"
     CDS             complement(19307..20173)
                     /locus_tag="U4B19_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEN2076782.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20333..22426)
                     /locus_tag="U4B19_001026"
     CDS             complement(20333..22426)
                     /locus_tag="U4B19_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2076783.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23175..23798)
                     /locus_tag="U4B19_001027"
     CDS             complement(23175..23798)
                     /locus_tag="U4B19_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076784.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(23957..25354)
                     /locus_tag="U4B19_001028"
     CDS             complement(23957..25354)
                     /locus_tag="U4B19_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2076785.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25386..26357)
                     /locus_tag="U4B19_001029"
     CDS             complement(25386..26357)
                     /locus_tag="U4B19_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2076786.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26731..27156
                     /locus_tag="U4B19_001030"
     CDS             26731..27156
                     /locus_tag="U4B19_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076787.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(27644..28345)
                     /locus_tag="U4B19_001031"
     CDS             complement(27644..28345)
                     /locus_tag="U4B19_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2076788.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(28361..28801)
                     /locus_tag="U4B19_001032"
     CDS             complement(28361..28801)
                     /locus_tag="U4B19_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN2076789.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(28801..29376)
                     /locus_tag="U4B19_001033"
     CDS             complement(28801..29376)
                     /locus_tag="U4B19_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEN2076790.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
BASE COUNT         8452 a         6120 c         5088 g         9775 t
ORIGIN      
        1 actgctaagc aaaagcagtg ccttgaaata ttttatttaa ataacacaaa aacataatta
       61 ctcataagaa accaattctg tattatctac catgaatgta aagaacccct cattaggcat
      121 cggatgaaaa taagcattga tcactcttat caaggcatta tggcaaacta acagatagac
      181 attttcttcg tcttcgatgc attctttcaa taaaggaacg atccgtgcat acacatctaa
      241 gactgactcc ccatttggga atcgtacagc gaacgctaaa cgtgcttttt gaaagttctc
      301 atctttgctt ggcagtccat cataatcccc aaaatccatt tctaccaatc gttcatcaac
      361 ggtcagcggc aagctgagac gatcagcaac tgcttgtgct gtatctcttg ctcgttgaag
      421 aggggaatgg atgattttcg tgattggaac aggcaaatct actacctttt ctgctagttt
      481 ctgtgcctgc atctgtcctt tttcagtcag cggcacatct gcccgtccgc aaactaatcc
      541 ttgtgcgttc caagttgttt ctccgtgtct cgttacataa agcatgtgat tctctccttc
      601 gttggcaaag ttccccttaa ccataccttg ttgttcttga gattacaagg gttagattct
      661 ctcaagagaa ataatctcag tagatacttt atccaggaag gtttgatcat gttccactag
      721 taacatggca ggtttcacgt tcaagatcag ttgttctaat tgttcttgat tgaagacatc
      781 caaataattc aatggttcat cccatgtata tagttcagct ggctgtgata aagatttagc
      841 caattccact tttttccgtt ggcccatact catctgctcg atcttgttat gaaaaacatc
      901 tctttccatc ccaagctttc ggaggttgtt caaaaatgct tggtagtcta cttggttttt
      961 ctctgcaaat tccgccaacg ttcctcgatt gtcttcataa ttttggcttg cataactaat
     1021 gctcagatgt tttggctggt atttttctcc tataaccttg ccgttgaatg cccccagaag
     1081 ataatggata atggatgact ttcctgcacc gttaggacct gaaatcgcca cccgctgatg
     1141 aggctcgatt gtaaaatgaa ttggctcgaa taacagattt tcatacccta attgaagatc
     1201 ttctacgctt aaaagtcgct tatggtgaga cgcttggcta ttcatcgtca aagaatcgat
     1261 atattcgata tcttttaata gtttttcttt ctcgctgatc tgggtctcca tccgattcac
     1321 gattgctttg gatcgtttca tcgtccgtgc agcatcagca cccactgctc ctttattcac
     1381 tcgtctagat tcagtatcga tgaatccgac ttgtttcttt gttttatctc cttctcggga
     1441 acgagaccat tcggcttttt cagctgctgt tttctttagc ctactgactt cttttttcaa
     1501 tttttcattt tgagccattt caaattcatc acgaagtttt ttctgttctt catagataga
     1561 gaaattccct tgataaagtt ccagttgact tttttcgatt gctaaaacat ggtccactac
     1621 ttcatcgata aatccccggt catggctgac cacgatgaag ccttgttttt tctttttcaa
     1681 ataagccgct acttgttttc taccagagat atccaaatga ttcgttggtt catcgattaa
     1741 cgggaaatga gtgtcatcca caaataaaag tgccagtagg acttttgtct tctctccccc
     1801 cgatagtgtg ctgaattctc tccataagat ttcaggatct gtttgcatca attggagctc
     1861 tctttcgatt tcccatatct caaaatccgt aatatcattt aacacgtaat aggttaaacg
     1921 ttctttatct tttgtctgtt gcgggaaata ggcaaattct tgctgatgga ttacttgccc
     1981 ttgataaggt agtttgtttt gtagaatatt cagtaaagtt gtctttcctc gaccgtttcg
     2041 tccgataagt cctagtttcc attgtgtgtc aaaatttaga tttgcttgtt caaataataa
     2101 tgtgccttgg ctgtcgtagc cgaatgtcag attttttatt tcgattttag acattttcca
     2161 tcacctcgta aaaaaagacc agtcctattt tcagaactgg ctaaagagga tgcaagggtt
     2221 ggatacacac aaaaagaccc atacatcgaa aaaaccaagc caatcccgaa aatctgcaca
     2281 ctgcttcctt gtctccacaa ggtacagtct gaatgcagct ttcatcaatc gacttaattt
     2341 ttcaatgcac ggatccctca ctttgtccta tatttgaaat tacgatagca taaagaaaat
     2401 gattttgtca actacttcgg gaggatggtg aaaaatctgc taagtgtctg tcctaagatt
     2461 tttgtttttt ctaatgtttg cctgtgttcc attgcttctt ctaaacctat tggacccaaa
     2521 tgaatactta agacaattgg gagaaaatca gaaagtcctt ctagctcttg gtctctactt
     2581 ccgcaaatag cgattattgg ttttcgatat ttttttgcaa gcactgctac accgtatgga
     2641 acttttccgt ggatcgtttg ctgatcgatc cgcccttctc ctgtaatgat gacatctgct
     2701 tgttcgatct ttttttccaa tccagtgatt tggctgacta aatcgaaacc agaaaccatc
     2761 ttcgctccca acaaaaaaag ggctccacca ataccgcctg ctgctccact tcctggaata
     2821 tcatctaaat ccacctccca gaaatctttt atttgctgag caatctttcc tgcttgttta
     2881 tctaagaaag ctagcgtcgt atctattcct cctttttgtc ttccaaattg ccaagcagct
     2941 ccttgttgac ctgtatatgg attggtaaca tctgcagcga taatcaattt tactttagaa
     3001 acaagaacgt ttcttcgatc aaaatgaagt tttttagtag ataataaagg atttccttct
     3061 gtgtaccctt ctaaagaagc acccaaggat tggagtaatc ctagaccacc atcgctacac
     3121 ccgcttcctc ctaaagtgac aataatttgt tctacttctt gaagcagtgc atctttaata
     3181 agttctccta aggcataact ggaagcaagt cggatagttt gatccgttgg tttatcaagt
     3241 agatctaacc ccaatgcttg tgcagattcg atgactgcgg tgttttttcc ttgccaagtt
     3301 gtcaataaat agctgatttt tcgctgacgg aataagagat ccatcccatc aatggtacgc
     3361 catgttcccc tgagcgcgta atagattgct tctgcagtat tttctccgcc atctgcaatc
     3421 ggcacattga tttttcttcc tttacgatct tcccagccat ttagaactgc ctgatttagt
     3481 tcaaatgaag aagcacttcc tttaaatgaa tcaattgccg ccaaaatatc catctgtatc
     3541 cctcactttc ttcttaagta agacactttt tttcattttt ttcaaacaaa acaatgctat
     3601 tttcttccat ttttctatat actaaaaggg aactcattga ataaaggaga gaaaaaacat
     3661 gaaaatcgca atagctgggg ccggagcaat gggcagccga atcggattga tgctacatca
     3721 aagcggaaat gaggtcctct tgatcgatcg ttggcctgct cacattgaag ccattcgtac
     3781 aaatgggttg attgctgact ttaacggtaa agaagtggtc gcaaaactgc caatctattc
     3841 accagaagaa atcatcgaaa gcaacgaaca cgtggacttg atcgtagcat taacaaaagc
     3901 caatcaatta gatgatatgt tttgttcgat ccaatccatt attactgaca atacttatgt
     3961 tttgtgttta ctaaatggat taggtcatga agatgtactg gaaaaatatg tgccaaagaa
     4021 aaatatctta tttgggatca cgatgtggac agctggtcta gctggtcccg gaaaagtcac
     4081 attgttaggt gatggagaaa tcgaactaga gaacttggaa ccagaaggcg aagcatttac
     4141 aaaaaaagtt gtagaagtct ttcaagaagc aaatttaaat ccgatctata gccataacgt
     4201 tcgttattcc atttggcgaa aagcttgtgt aaatggaaca ttgaatggtt tatgcacgat
     4261 tttggattgt aatattgctg aactaggcgc gcaaaaagcg gcagagagca tggttcgaac
     4321 aatcgtatct gaatttgctt ctatcgcagc aaaagaagga atcatactag atcaagaaga
     4381 agtgtaccaa catattgctt ctacttatga tcctgacaac atcgggttgc attacccatc
     4441 tatgtaccaa gacctgatca aaaaccatcg tttgactgaa atcgactaca tcaatggtgc
     4501 catttggcga aaaggacaaa aatatgatat tgctacacca tattgtgcct ttttgaccca
     4561 attagtccat gcaaaagaag gaatccttgg cgcagaataa aaggctctct gatgagaatc
     4621 agctgaatca gcagctggtt ctcttttttt aagcccaata aaagatcaaa aagaaaacac
     4681 aactctcatg atgccataag aattgtgctt gtttgtttca gtacataatc tcttcaaaat
     4741 gtaggttttt tatttgtttt caacagagga gaggtcatat tcgtattgct cataaatagt
     4801 ggaatcgtta gtgataaatc gttttgccca attttctgca tcataaaatt ccaatagctc
     4861 tgataggatt ggcttaaatg gtgtatgcag aaattccggt aattcatcta aatcacgttc
     4921 tcgaagcaaa aaaatatttt caggacgata aaaatgataa tgagcaatcg cttgattatc
     4981 cgtcactagt ttcttttgaa aatacatgga ttcaaatgtc cgcaaagtca ctccttgctg
     5041 accctcccgc aacagttcca gaatcccgcg cgattcccct gtttttttca ggtactcttc
     5101 ataagtaaga tatccaggtt gttcattccc tcgatgtgga agaatgaaat agttggtcgt
     5161 aacatctctt tttataaatt cctgcttata gctttctgct cgttcttttc tcccattatt
     5221 cgttgcacca aaaaatagat ccgtctttgg ttcgctagaa gaaaaattca tatgtttcga
     5281 ataaaatgaa ctattatgtt tcagcccaag ttcttttgct tccaaaaaat caaaatgata
     5341 aatctcatcg attgccggtt catcttgtgc ctgcttcagc atcgcctcgt gttctgcttt
     5401 gaagtgattc caaaaaaata acacaatttt tgcattggta aatgggcgca cgcgtcgagc
     5461 catctccata tcaaatccat aatctaaaaa aacgatcagc tgataatttg ccaactgttg
     5521 tctagctgat ttaatgaatt ccgactgtag taaatctttt ccggaccaaa catctaaaga
     5581 gcggatggaa ggcaaaaaat aaagtgattt attttttgca actagcagta cgtcattttt
     5641 gttcatcttt tccgcctcac tttctctgtc actttttcct atgatagcct gtttaaatag
     5701 taaaaagcca gtaaaacgtt tcctatattt gtatgaaaat ttaacatttg tatgaaaata
     5761 tgatgaaata aatcattctt ctattaaaat tcgcctaaaa gttttctgaa tagttgtaat
     5821 tgtctgaaaa tttgttatca ttagtcattg ttaaatattg ctatttattc ttaatataaa
     5881 ttttgattgt gaatttaaaa gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca
     5941 acttagctcc agacatatca ccatgttagc tttaggtgga gcaatcggtg caggtctatt
     6001 taaaggaagt ggcgaagcga ttgggatcgc cggcccttct gtattgattg cttttttgat
     6061 tggtgggctg attttatata tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg
     6121 cgacacgcat cacggattat ctggtttgat acgcccttat ctaggtgctc attctgctga
     6181 ttttactgat tgggtctact ggtctatgtg gattatcaat atcatcgctg aagctgttgc
     6241 agctgcttcc tttttgcagt tgtggttccc gcacattcca gcctgggtat ttgtttttat
     6301 gttggctgtt ttaactacga tcatcaatct ttattcggta cggttattcg cagaaacaga
     6361 atattggctg gcttttgcta aaatcagtgt cattatctta ctgatcattt ttgctactta
     6421 tcttgtcggt cagcaaatgt tgggcacagg cgttttccct acccttcagc aattgactga
     6481 ccatggtggg tttactcctc atggtatgaa gggaatcatc agttctttgc ttgtagttat
     6541 ctattcttac ggcggttctg aactgattgc tatcacggta agcgaagcag atgatcctaa
     6601 acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga atcatttcct tttacatcat
     6661 tcctttgttt ttgcttttga ttctttttcc ttggaatact ttagcaggca cgaatgtcag
     6721 tccttttgtg atggtatttg aaaaaatgaa tatcccattt gcagcggata tcgtcaattt
     6781 tgtgattgtc cttgctttgt tctcatctat caattcgggt gtgtatgcat cttcaagaat
     6841 tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga aaactagctg ttttaaacaa
     6901 gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt gttctttatc ttggtgtagc
     6961 tctttcttat tttgtcggtg acaaattatt tggctatctg gcaggttccc tttcgtatac
     7021 agtattattg atctggatca ttatcagtgc ggcatccttt gtactggcac taagaaaagg
     7081 aacattatgg gataaaggaa tcagcttttt tgcattagct gtcttaggat tgattttcct
     7141 tggtatcctg ttcacgaact ccattggggt aactgtattg actggcctgc tttacctgtt
     7201 catttatttc agttatcaaa aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg
     7261 atagaaaatt ttattttatt tatgtggaat agatggagct attgaagaaa ggagttgtga
     7321 caaaagtctt gttgcaactc ctttttccga ataaacagtc tcatgacctc ttggtaaacg
     7381 gtgttcaaaa accctctgtg caaaataatt gattgttctc catgctcatg acatgataga
     7441 agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa gtattggaag tttttgttga
     7501 tgatcggtgt ttcaacggta gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg
     7561 atcatatctt cttcagtcgt acacgagtat tcatggcgtt gatgatgggt gcagtcatgg
     7621 cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa caaaaagata aatgttatga
     7681 tcttagccgc tagtcttctt gtttttgccg gttccttgtt tatggtacga agccaaacta
     7741 ctgtcaacga cacagcttgg atgaaggcca tgattcctca tcattctatc gctatcctgg
     7801 caagtaaacg agctgattta aaagatcctg aagtaaaaaa attagcggaa gaaatcattc
     7861 aggcacaaga agaagagatc gacaaaatga aaaaaatgat caaagaaatg gaatgaccat
     7921 tatccgtcca aataaaaaaa gaggatgttc cagaagttat cagcttcaag aaataagaag
     7981 aagctacttc tggaacgccg tgaataaggg gttgtgaaaa agctgccctg catcaagtaa
     8041 taagaacagc caaacaaaaa tagcttctcc tatttttcgt ttggttaggc ttattaccgc
     8101 agatgcaagc ttttgaacac cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag
     8161 tattagagag acaaatggaa aaatagttcc tcatattttg ccattttttg agctaatacc
     8221 gagggtcagc tgattgaaca ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc
     8281 gtattaagaa aaattttgtg aaatggcttt ccacattttt gtataaattt ggcttaatac
     8341 cgagaaactg gcttttgaat accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc
     8401 acaatccctt tttatgtttt tattgttcta attgcgctgt cttgcttact gcttcttgcg
     8461 gtttcacaaa tttttttgag attccccaac ctgtgaaggc actgaccatc gaaaacacag
     8521 gagaaagcaa actgaaaaat acgaatggca agtaagtgag tgtaggaata ccaaatgtac
     8581 ttgctacgaa acttccggca attccccaag gcactaagta gttgattact gttccaccat
     8641 cttctagcgt tctacctaat acagttggat caaatcctgc tttttcataa gtttctttaa
     8701 atgcattacc aggcagaata acagatagga attgttcacc aataaaaata tttactccga
     8761 tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt cgtcatcttt cttgatactc
     8821 gttccatcac ggttgagatc aagccaaatt cgatcaacaa tccacctaat gacaaagtta
     8881 gaataatcaa tgacacagta ggcatcatac tttcgattcc tccgcggcta agcagtgaat
     8941 cgatttgttg gtttccagta tttgaaacaa accctgattc gatgacccca gccaacttcg
     9001 ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac tgctacagcg atattcaaca
     9061 aaatcgttaa aattgccggc acctttttcc atgcacacag taacattaaa aataaaggaa
     9121 tcagtgacca gattgaaata gtaaagtttg cttctagaac atcagtcact tcacgaatag
     9181 atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa
     9241 caaatgccgg gacagttgac cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa
     9301 caatagctgc tgctagattt gttgattcgg ataatggaga catcttatct ccaaaaacgg
     9361 cacctgaaac aatagctcca acaaccagcg ctggatttaa gcctagtgtt gtcccaatac
     9421 cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc actccctaca gcgctaccta
     9481 cgattgcaca cacaacaaat acagaaggga caaaccactg gacactgatc aatttgaacc
     9541 caatgaccat tagagttgga atgatccctg cttgtatcca tacagcaatc aatgaaccga
     9601 ctaaaataaa aataaagatt gggatgattc ctggtttgat gccattgatg attccttcgt
     9661 gcagccaatc tgtcgggtat ctttttatca ctgcatacaa catgaccatt ccgatcgctg
     9721 ctaagatcgt gatattaggt gagaccttta atccgacaac gccaatcgca atgcctaaaa
     9781 caataaccat taatacaagt gttgcttcta aaaaacttgg tgttcctttt tttcccatga
     9841 taatctctcc ttttttagtt gttcttatgt tctaaaaaag ttaagatatc ggattactcc
     9901 gccgcaattc atccgcgatt cctcctttat aaaaataaaa agacctgttt taaactaagg
     9961 acgaatgact ccgcggtacc accttaatta aaaacagatc tgtttttcac tttcaaactt
    10021 ttattcaaat gtatggactt tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg
    10081 accaccgact ttctgaacaa aacacctaac cgctactgcg tgaaaagttg acttgtacga
    10141 agtttacctt ccataagatc atttgtcaat gaaaaagaaa atattttttc atttttctga
    10201 aaattatttt tataactttc acacacaagt ttatttttta ctagaaagta gcgccctctt
    10261 gtttttttct tgctactatt tcttgatgtt cttgcttgat tctttctaat agctttggct
    10321 ctttcaacag atcaagagct gtattggcga gtagttctgc tgcaatcgca atagaagcaa
    10381 ggcctttgtc acttttagca gcagctttga attcttctgt gtgtccggca atatatgtct
    10441 cagagattgc taccgtaggc tggattgttg gtatgacttg gctgacattt cctacatcag
    10501 aagaaccgag acttgttctt tgttccgttt ctatttcttc atctggaaca cctgcttctt
    10561 ttgcatgctg gaagaataag tcatcaaaag aaggagtaac gatgacatca tcaactgcat
    10621 tttggaacaa tccaaactca aaagtcgtgc ccgtacttat cgcagcacct cgtacgatat
    10681 tttccacttt ttcatatact ttatctaatg tttgccgatt tcctgcacgt aaatagaaac
    10741 gtccagcagc gtaatctgga atgacgttgg cagctactcc tccgtctgta atgatcccat
    10801 ggatattgac atcttttggc aattgcaagc gtagagcgtt gattccatta aacacttgga
    10861 tcaatgcttc caaagcattt actccttttt caggtgctgc tgctgcatgg gaagcgacac
    10921 cgtaaaattt gatgtcgact ggatcattcg ctaaagattc tgtcgttttc ccataacgaa
    10981 aagctggatg gacgcataac gctacatcga catcctcaaa aaaaccttct cgaacaaagc
    11041 tgcctttagc tgatccgttc tcgccccctt cttcccctgg ggtgccatat acacggattt
    11101 ctccaccagt ttcatcaatg aattgcttca ctatgctggc ggctaatacg gagtaagtac
    11161 caaacaaatt atgcccgcat gcatgaccaa tacctggtaa agcatcgaat tctgccaaaa
    11221 aggcaattgt gggaccgggt ttattggaaa tataacgtgc atcaaaggct gttcggtgtc
    11281 cagctacttc tttttctacc caaaaccttt ctttttctaa ttgtttaatc aacgctgctg
    11341 aggaataaaa ctcatagtta cttacttcag gatgttcgtg gatatctaga gcaagtgctt
    11401 gatattctgg taaacgatat tggattagat ccttcaattg gtttgttgag attgttgttg
    11461 tcataaaaaa ttcctcctat gctagtcatt tttcttattt tctcccgaat gtttcccatg
    11521 caggcacgct tgatcctttt gatgttttct cgattacttt gactgtgtct tctgtttgat
    11581 aagcgtctac tattttttga tacgttttat tgttttcatc ttcttttctt gcaacgatga
    11641 tattcacata aggacgtgca tggtcatcca ctggttctaa gaagatggca tcagaagcag
    11701 gattaaatcc agcatctaca gctacaccgc tattgatcac tgaaatgtct acatctgaca
    11761 aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc tagatttttt ctattctcgg
    11821 taatatcaga aacagtgggc gcttgtcctt ttgccttatc cactttgatc aatccagcag
    11881 tttgcaataa cagaagagct ctgccgccat ttgtaacatc attagggatt gctacttttg
    11941 ctccgtcttt gatatcagct acatccttca aagtgttaga ataaattccc agcggtgcat
    12001 tgacagtatt tccgatcgat actaaatcag ttccgtgttc tttattgtaa ttgtctaaaa
    12061 agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc tgctaaagcc acatttggtt
    12121 gattgtaatc tgtaaatttc acgtattcca aattaatgtt ctcctttttc aaacggtctt
    12181 tcacgttgtc ccatacatct gtatcttctc caataatccc tagttttacg atagtttctt
    12241 ttttgtcagt tgtatctgct tttccttggc tacaccctgc caaccaaata attgctacaa
    12301 atgtaatgac tacaaaacga ataatttttt tcattttttc tcctccactt tcaatgtgta
    12361 atttttttaa tcagttgatt actgataaat tgacttaaaa aaactaagat tagaatcaat
    12421 aatgtagcca caatcgttac atctgtttgg aatcgattat accctctcga tatagcaaga
    12481 ttccctaatc cgcctgcacc tactgctcca gccatggtag tcagtccgat cacgttgatg
    12541 atagtcaacg ccgagacccg aatgatacca ggcaaacctt ctaacaaata cacacgtaaa
    12601 atgataccga ttggactggt tcccatcgct tctgccgctt cgatcacccc aggatcaact
    12661 tctagcagtg cgttctcgat ttgacgagca aaaaacggaa caactccaaa aatcaatggc
    12721 actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga aacgagtagt caatgctaaa
    12781 agagaaagca agataatgaa agggatggaa cgaatgatat tgatcagctt atcaagtatt
    12841 tcaaatagca gacggttagc tagtaatcca ttagggcgtg tcactactaa cgtcacacct
    12901 aagatgattc caatcatccc gccaatgaat gctgtccaaa aagacatata tagtgtttgc
    12961 ttcgtcgctt cgacaaattc atccggaatc aaataaacat tgggcaaata tgttttaaag
    13021 aaatcagtca ttttatcttc ctccttcgat cactttgata cgactatcta aatcgttttt
    13081 ttgtaaaata tttattttta caccagattt gatcaaataa tccaatgctt ttttccgctg
    13141 tttggtttct ccagctaaag taacgatcaa actccctaac ggcgtttctt gtaataactc
    13201 tacattgcca tataaaatat tcgctgttac ttgatatttg ctgtacagat gagcgattag
    13261 aggttcgttc gtctgatttc caatataact taattccacc agccatttat tggcgatctg
    13321 atcggcaaaa gcagaatggg caatgatcgt ttctaatgct tggtcaagat gagtagctgt
    13381 tcgaataaaa tctttggtca attcttcttc aggattgctg aagatctgga tgctgctacc
    13441 tttttcaacg attcttccat cttccataac cgctaccttg ttgcagattt ctttgacaac
    13501 ttgcatctca tgagtgatca ggacgattgt taaacctaac tggcgattca atttttttaa
    13561 caatgcaagg atctgaaggg tcgttttcgg atcaagtgca ctggttgctt catcacataa
    13621 taatacctta ggttcattcg ccaaagctcg tgcgattgct actcgttgtt tttgtccccc
    13681 cgacagttgt ttaggataag cagatgcttt ttcttccaaa ccgacaagtt ccagtagttc
    13741 attcactttt tgacgacgtt cctgtttcga ttttccagaa tactttaaag aaaaatcaac
    13801 gttgtcaaaa atagttcggc tttccattag attgaaatgc tgaaagatca taccgatggc
    13861 ctttctttct tttcgcaatt ctttgggaga gagctgactc agattcgtct gattgacaat
    13921 gacttctcct gctgttggtt tctgcagcaa attcatcaca cgaactaacg tacttttccc
    13981 tgcacctgag taaccgacga ttccataaac atcgcctttt tcaatcgtta aatctacaga
    14041 atctactgcc tgtatctctt tttctttttg tttgaaacat acagaaacat ctcgcaattc
    14101 aatcattaaa attcctcctt atctaaaaaa agcgtcctca agaaaatgaa aatgtcattt
    14161 tcttgaggac gcttttttgc gtgttaccac ctcatgttca agctgtgttc acacacaact
    14221 cctcttcaag tacgatcata ctctagcaag atatcggtcg cagccgtcat cgccttacat
    14281 ctatgctcgg gatgaattgc tcgatggcca ttttccaata gtcgttcctt gcttcttctc
    14341 agctaccgaa gctctctgtc aaaggttctt ctatcgtact taccatttca atgcaaataa
    14401 tattcaaaaa aaaagtcctt ttactaacat aaactatgtc agtaaaagga cgacgtatcg
    14461 cggtaccacc ttttttcgga agtttccttc cctcaagccc aacatctcaa aaagagagat
    14521 tggcgttcgt tttaacggtc gaacagttcc gaatttgcct acttatcgac aaattattct
    14581 ccaaggccat tttcagcttt tcttacttga ctcttctcac cagccagagc ctctctgcaa
    14641 agtttccaaa cttactttcc ttttcaacga aatcgatatt ttgttgttgg gattaatcta
    14701 catgaggaaa actgaaaagt caagtacgaa attcaaatta ttttttctat atttattttt
    14761 ctgttttttt atttaacatt tcaataacag ttcgaagatc ttttccttcc gcaagaagtt
    14821 tcaatttttc cttttcttcg acggtataat ttcggacaaa actggaatca ggtatcaaaa
    14881 caacgcctaa tcctgctcgt gaagcagctg ttgctcctac taaagaatct tccactgcaa
    14941 aggctgaact agcttctaat cctgttcgtt ccaaagctgt cagataagga atcggtgaag
    15001 gcttgttctc cgacacttcg tctccgaatg taaacgtgtc caagtaatca gctaatttaa
    15061 agtgatccaa gatatctgtt gctcttttcg taacagtggt cgtagcaatg cctagtttca
    15121 tatgacgttc ttttaagtct gccaatattt cttttgcata tggttttaag tggatggctc
    15181 cgcttgtcag ctgttgataa aaatattctt cacgcttcgc tcggacttcc tgtacttttt
    15241 cagggctacc tgctgctttc actaaaatat caaaactttg tttccagttt tttccgcccc
    15301 aagcgtcaac gatttctttt gggatatgga tatcaaattg ctgaaatccc caaagccagc
    15361 catcacgata cgttttttct gtgtcaacta acaaaccatc taaatcaaat attgcacctt
    15421 tcatctatac attaatcctt tccaaaacaa tacacatagt ttagcacgac tggtccaaaa
    15481 agaacagaag caaagaacca gccttctcgt ttataagagt tcttggaatc gacggatata
    15541 gattttcttc acagctgtca caagcgccag ataagcgatg actgtagcaa ataaccataa
    15601 ccagtaattg ccaggaagtg gcatcaagcc taattcagca ccaaaacttg taaaaggaag
    15661 gacagaaccg acaataattc ctaatgtagt gataacaaac atggcaaatg ttgcgttgct
    15721 ttggataaat ggtactttcg gtgtacgcag tgcatggagc actaaggttt gtgtccatag
    15781 tgattcgaca aaccatcctg catggaataa tgcgatgaaa atcactcgct gtgaactatc
    15841 caatgtatgg aaacttccgc ccactacagc aggacaaata acaaaataca tcaacaagta
    15901 agtagtaata tcaaatatcg aacttgttgg accaaagtaa accatgaatt ccccaatact
    15961 agatgcattc catttttttg gctcgtccaa atattcttta tccatccgat cccatggcaa
    16021 ggaaatgcag gaaacatcat agatcagatt caaaaacaaa agttgtaaag gcagcattgg
    16081 taaaaatggc aggaacgtac ttgcaatcag cacggaaaac atattgccaa aattagaact
    16141 tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt tctcgtccag aaagaaggcc
    16201 tctttccaaa atcgtcaaat ctttttctaa aaggatcacg tctgcagatt ctttcgcaat
    16261 atccacagct gtatctacag aaataccgat atcagccact ttcattgcag gagcgtcatt
    16321 gataccatct cctaaaaatc cgacagtatg accattttta cgtaaagctg tcgtcaaacg
    16381 tgctttttgc tgaggattca attttacgaa tacttcatgg tcttcagcta cttgtgctaa
    16441 ttcatgatca ctcatttccg agattttctc accggtaacc aattcattga tctctagacc
    16501 tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc ccagtcagaa cttttacagc
    16561 tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt gtttcttttg gtggatcaag
    16621 gaaagcaaga taaccaatca aaaccatgtc tgactcatct ttgactgaaa attctccaac
    16681 gacgcttgga ttggtctttt gtgcgacacc aatcactcgt aagccatctt cattaagtga
    16741 ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt aatggtgtaa cagtaccgcc
    16801 aagatcaata aatttagaaa tgttcagcat ttcttcaatc gcgccttttg tgatcatttg
    16861 tgttttacca gctggatcag caaccacaac actcatacga cgacgttcaa aatcaaaagg
    16921 aatttcatcc actttttgat aattgatctt atctgtatct aaagtttctg ttgcttcatc
    16981 aatgatagcc acatctaaca aatttttcag ccctgtttga tagtagctat ttagatacgc
    17041 atggcgcaaa acacgatcat cttcatttcc ttcacaatcc atatgatatt caagaatgat
    17101 tttatcttgt gtcaatgtcc ccgttttatc cgtacataac acatcgattg caccaaagtt
    17161 ctggatggca ttcaaatttt tgatcactgt tccttttttg gccatcacac ttgctccttt
    17221 gaccaaattc gtcgtaacga tcattggaag catttcaggt gttagaccaa ctgctacaga
    17281 tagtccaaaa agaaaagctt ctagccaatc cccttttgta agcccattga tgacgataac
    17341 tgctggagcc atcaatgaca taaatttgat cagtagcatg gatgttttat ggatgcctaa
    17401 ttcaaagctt gattgaattg gtttttcaga tagcgtttct gctacatgac caaatagtgt
    17461 ttcatttcca acagaaacaa tgattccttc cgcacttcca ctgatgacat tacttcccat
    17521 aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg tcttcacata ctttcgcatg
    17581 cttttctact ggatagcttt cccctgtcaa tgctgcttgg gaaatgaata aatcctttgc
    17641 ggacaataaa cgcatatccg ctggtatcat atcccctgct gaaagccgaa caagatctcc
    17701 acagacgatt tcttccatag gaatctctgt gtactttcca ttacgtttta cagctgccgt
    17761 tactttcaca agtgatttca gtttttcagc cgcttgattg gaacgtacgg actgaatcaa
    17821 cgtcattgtc ccgctgacga taaccatagt gaagataatg gctacaccga aaagatcttt
    17881 atctccagga gctgctaata catattccgt gatgaatgaa ataattccta atgcgatcaa
    17941 cactaatgta aatggcgtga tgtatgcttt caatatttct acaatgaatg gtgttttttt
    18001 gccatatgaa atcgcatttt caccgtaatt ttctcttgaa cgttcgattt gttcttctgt
    18061 caatccattt gctgatgcat ggaatagttc aaataattct tgtttcgttt ttccagcaaa
    18121 agctgcatag attttttgtg tatctttctt tttttccatc tcaatctctc ctttgtgtcc
    18181 aaaagacagc tgttctcgct gcttttggtc tttagctatt catcggatcg atagagaaaa
    18241 aactgaaagg ggctgagaac agatagataa tagccctggc ctttcctcta gatccttgtt
    18301 tttcgaatga ctgtcatcca tttgttctca tctcctttac taagtaattt taattcatga
    18361 cttccaatca tttttcgaat agttattttt ccaattagac cacctccttt ttatttttca
    18421 ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa tactcagcga aaggtttgaa
    18481 aaagcatatt aaaaaaagct tctcagatct catcgttgag ttttgacact gtacaacgta
    18541 aaaagcagat acctgctttt agctacctta tgaaaagcct tatctcgaca attcctgttc
    18601 tacccattgg cgtctctcga cgtttttggg cagtagccta tgtttgtata ggagcctcac
    18661 ctaacaaatc ttattcggtt ttccttcgtt aatataacaa ctaaaaaaga ggatgtcaaa
    18721 tcaattttcc atgtttttca tacttttttt aaaatacaca cgttatattc ctgtctcacg
    18781 cggttttatt tgtctcattt tccacctccc gctataatag aaatatagta ggaaacgagg
    18841 tgctgttatg gaaagaacac ttgttattat caagccagac ggcgtgagac gccatttagt
    18901 cggaagcatc atccaacgtt ttgaagcaaa gggactggcc attgctgaaa tgaagtttga
    18961 aactatgact cctgaattag caaaagaaca ttatcagcac ttgactgagc gcagtttttt
    19021 tgatgaactg atcgattaca tgacttccgg tcctgtcgtt tatcttgtgc tggtcggcga
    19081 ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca aaagctgccg atgctgttcc
    19141 aggtacgatc cgaggagact acgcgctgcc ggggactgaa aacatcattc acgcttctga
    19201 ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc ccgccattcg atataaacaa
    19261 accgtctgaa aaagcggcga agtgatacaa attctaaact gctggtctag ttgctgggtt
    19321 cagcaatggc cagcagtttt ttctctaagg acctccgttg attctttcct aacatatatt
    19381 cgagcactgt gaagaattct tggtagtctt tcgtcttttc ttcccctaaa gcagcagaaa
    19441 tcaatctttg ataataatct gccatcaatg tttcatagat cactgtactt ctttctttat
    19501 gtgataagct ccgtttcata tcttcattca cactacgtgc ctttttcaac cgaccatttt
    19561 ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag atactgctca tgattgttca
    19621 actgtgctaa tcgtcgcatt ctttttttga ttttcaaata attgttctca atgaaccgat
    19681 cactgaatat ccaaggaaaa tcagctagaa tagatagttc aaattgacca tagtctggta
    19741 tctgcatcaa gtattcttca atgattcggc attcataacg taaaaattca aattctgtct
    19801 cagatacttc tgttttttcg accatgtgaa gattcaaaac caaataatac agatcttctg
    19861 ttttcttaaa atgcctacgg ttattgctga tctctttata aggatcaaag ctgtcttctt
    19921 gtacccgatc tagcattcgt ctttttttct ttaactttat tggttcatcc agctggtttc
    19981 gcatccattc atattctgca agtgtcatgt tcattcgctc taaaaatgat ctcaatgtta
    20041 caaatgacat ttttttcact gaatttgtct caatcccgac tagagaagtc ttaaaacaga
    20101 ttccttccgc taattgggac tgtgtcaact tcttgttgac tcgaagtttc ttgatcaatt
    20161 caccatcata cataatagtt ctccttttta atttaaattt ttccacattc aataaataga
    20221 tacgttaaaa aggaaaaata cacgaaaaaa ataaaaaaat gtttttttca taaaaaatac
    20281 caaccttcaa gcattagaat gttgtctaac tactgaaggt tggtttgatt gtttattgat
    20341 tatttttttg ctgttcaacg tctgttcttg ttactggata taagttggtt gttgttttat
    20401 taccattttt tgaaaagaca cttgtcgaat gttcgccttt ttgcttttcg attgcttcat
    20461 tacgttccgc ttcattttta tagtcatatt ttttcggatc aatcgtctca aatccgtttg
    20521 gcacataaaa ccgcaagaga ttttcctgat tcagcttatc tgaaagactt aacgcagtct
    20581 tgactttttc ctgatctttg gagatttcct catcgattcc tgctgctttc gtatcgatga
    20641 tttctcctgt ctgattattg taagcttttc ctcctaggac ggtgtatttg ggtgtcacaa
    20701 agttcccatt tcgaaaagca actacttgat catgctgaga agataataag tctgtaccaa
    20761 aatgaagata atctttatca tcgattccca atagatgtag taatgtcggc aaaacatcga
    20821 tttctccacc gtattcatga ttgactgtcc ctttagtata accaggcata tggatcatca
    20881 atggcactcg ttgcatctgt gcgttgtcaa attcatccca tgtatctgga tctttaccaa
    20941 gagcgcttgc taaatcttta ttatctgtat tcgagatccc aaaatgatcc ccgtaaatca
    21001 caaagattga attttggtaa ataccagatt ttttcaaata cgtaaaaaat tgttccaatg
    21061 attgatccag ataatgtgcc gttcgaacat aattatctac tgtgctattg ccagtattca
    21121 aagatggaaa gtcgaaattc ttgtcatccg tataataagg cgtgtggttg gttacggaaa
    21181 gaaatttcgt gtaaaacggc tgttgtagat gctcaagata ttgggcagat tctctgaata
    21241 aatctttatc taatatccca taacctaacg tttcgtcgga ttcatcaaaa taagatcgat
    21301 caaagaaatt gtcataacct aagtttttat atacatgatc tctattccaa aaactagcta
    21361 cattcccatg gaaaacagcg cttgtatagc cttgcttttg tccaaggatc gctggtgcgg
    21421 cttgaaagac attgtctgac ccaagttctg tgaataatga gccttgaggt aaaccaaatg
    21481 tgccagtttc caacatattt tcagcatcac ttgtctttcc ctgtcctact tcatgaaaga
    21541 aattgtcaaa gctgatcgtt gcttgattct ggaaaatact atttaaaaac ggtgtgactt
    21601 cttgtccgtc taccttcata ttaatcaaaa actgctggaa actttctaaa tggagaacga
    21661 tcacattttt ccctttagcg atcccaaatg tttcgggatt tggctctgca taatgtttct
    21721 ttgtataatc taaaacttca tcaatcccat tactgcttgc atgcgcgcga acactgctag
    21781 tcattcctgt tttgattccg tcataaatag tatacgcatc gattcctaga tatttgacaa
    21841 tatatgaacg gtcaaatgtt cgctgcagca attgtggacg gttggcttcg ctcagcgcta
    21901 gatttcctga aaaaataagc cctgaaagac aggtaacagc gatagccatc ggtttgccga
    21961 cagggttgct tttgataggt attttcttga ccttcaacca aaccagtagc ccaatgaaca
    22021 cagcgatatc cagccagtag atgacatcat ggggcttcat caaagaaaag gagcttcctg
    22081 acaatccttg cgacactttc gaaaagccta atacagattt gactgtaatg aaatctgtga
    22141 attcccgata aaaaataata ttcaagtaca acaaaagcgt atttaaaata tcaatgatca
    22201 aaagaatagc taacgctggc tttggctttt taatatacaa agaaaggcca aaaaacaata
    22261 aggtagtcgc aattgggttg atccacaaga taaagtactg gatcgtacct tccacaccta
    22321 atgaaaaatc tgcatagtag gcaatgatcg tcttcagcca gaaaaagaaa acagctgtca
    22381 aaacgattgc taaacgattt tggcacaatt ttttaagttg tgtcacgtat atcacttctc
    22441 tctattcaca ttttttctta ttttgctcct gaaaaatgaa aatttcctaa aaaagttgtt
    22501 atcttaagat ttttttattc tttctaaaga ttattccgtc cacttttcac agcataaaac
    22561 cgctgcgatg taagcgatta aactaatgaa gagataaaag tcttgtggtg accgcttcac
    22621 tccacggtat tcaaaagcca gtttctcggt attcgtacaa aaaaagaggc tgtgacaaaa
    22681 ttcttgtcgc agctccttat ttcttgatgc aggacagctt tttcacaact tcttccttaa
    22741 acggtgttct cctgccaact cctcggccac aagtcacaat atttataaaa catgagaagc
    22801 tgttttttga aattgttcct tgtggctcag agttaaacgg caggctcaag acctcttgat
    22861 acacggtgtt caatcagctg accctcggta ttagattatg tcaggacgct aattttacaa
    22921 cctcttggta aacggtgttc aaaagcttgc atctgcggta ataagcccaa ccaaacgaaa
    22981 aatataagga gctatttttt gaaattgctc cttgtggctc agagttaaac ggcaggctca
    23041 agacctcttg atacacggtg ttcaatcagc tgaccttcgg tattagctca aaaaacggca
    23101 aaatatgagg aactattttg ccgtttgttt ctctaatact caggtcaaaa cgctgatttc
    23161 acaacctctg tttttcattg tccataggat tgatattgtc cttgtgatga ctcattcgta
    23221 ataattgagg cttgattttc ttcacttccc gttatcgttt gtccactgcc agaatctgaa
    23281 ggacttgttc ctgtcgactc gtttttttga gaagagacag ttgttttctt gacttgttca
    23341 tttgtctttt tccattccgc taaacgatcg gcgaccactt ttttgtctac tttttcagaa
    23401 taagcggtga tcacttttcc atttttcaaa cgggctactt tgtcgccatc atgagtcaaa
    23461 agcaaaacgg tcaaatgctt gtctttaaat tgatattgtc tgagagaaat cgcactaagt
    23521 acgtcatcga tattaataaa ttgattttct tcttttcctt ttggcaaata aatgtccaat
    23581 tcgccttttt cgtaattggc agttatttta tactccgccc accaataagt tgagaattct
    23641 tttttgaaat tatctactga ttctttgctt gctacgattt tttctgtttc tttctgagca
    23701 agatctgctg tcatttttcc tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg
    23761 aataaccaaa agagaagaaa cggtcttttt ttcctcatgc tttcctccac tcctatttaa
    23821 ttttgactat tctattatat acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat
    23881 cgtaaataat tgaacttctt tttcacaaaa tggtaagaat ggagaatttc acgcatttgc
    23941 tctcaactat tctttcttaa gcctcaaatc cgtttttatc tgatacgtct ttgaaccaat
    24001 gacccgattt tttgattgtt ctttttccat cttgtgctaa gtctactgaa ataaagccat
    24061 atcgattctt atatgcattg gtccacgacc agttatccat acatgtccac atatgatagc
    24121 ctaaacaatt ggcaccttct tgaatggctt gatggacata tttcagatga gattggacaa
    24181 attcgatccg ataatcatcg tggatcatgc catcttcttt gataaatcgt gcttcgtttt
    24241 ctacacccat accgttttca gaaataaagc agtcgatatt tccataatga tctcgtacat
    24301 tgatcaaaat atcatagatt ccttgttcat agatctccca tccgcgataa ggattcattt
    24361 tacgaccagg catttcataa ttgtcgaaat aatcctcagg cattggaccg tttttagtat
    24421 cgattggtgt ttcttttgct tttacccggc gtggctggta ataattgata cctaaaagat
    24481 ctactgtatt ttctttgata accgtcaaat cctctgcatg gatcacaggc atgagatcaa
    24541 gttcttttac tatctcaatc aattcacttg gaaactcgcc ttttacagct ggatcaagga
    24601 atgaacgatt gaagaacgca tctgctattt gcgctgcttt aacatcttca gggtttgatt
    24661 cgtctcttgg atagcttggt gtcagattca agatgatacc aataacacca ccttgctcca
    24721 tttcatggta agatttgatg gctaacgctg aagccaacgc ttcatgatag ccaacttgga
    24781 tcgctttttt catatctgac tcttctggat aatgccatcg atataaataa cctgcttcga
    24841 ctggaacgat tggttcatta tgagtaaacc atttttttac tttcgaacca aacaattcaa
    24901 aacaactttt ggcatatttt tcataggctt caaccgtttc tcggttcaac catccgcctt
    24961 tttcttgcag atacatcggc atatcgaaat gatacagatt cacaaagggt tcgataccgt
    25021 tttcaatcaa ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg ttgattgttc
    25081 cagattcatc aggtatcaaa cggctccatt ggatagatgt tcgaaatgaa ttatgcccag
    25141 tctgtttcat caactgtaca tcctcttgat acttcgtata aacttgcgag gttttttcag
    25201 gtcctacttg atcaaagaac ttttgcggtt cttttttgta ccaataatca aaaatgttct
    25261 ctccttttcc atcgcctgaa atccttcctt ctgtttgtgg gccacttgct gcagaacccc
    25321 accaaaaatt tgctggaaat tgatattttt tcatcaaagc acctcttttt cttttttagt
    25381 tcgttttagt tgattccatg tatgatattt tccatcacaa tcactttttc attttcttcc
    25441 tcttcttttt tgattcgttt taacagctgt tctgctgctc gttccccgat tttatctgtt
    25501 ggtggcgtga tcgtgatttt cggaaggata ctcagatcgt ttttatctaa ataatcatag
    25561 tgactgaacg caatatcctt cccgattctg acaccttctt gattgaaaca ttcgatggca
    25621 ccaatcgaca tattgtaatt gctgacaaaa atagcagttg cccctttttc taataatgct
    25681 tttgccccgt aatagccgct ttttctagaa tagtctcctg taaagacctc taattggatc
    25741 gatggatcat tttggaccgc ttctttggct cctgctaaac gttctcttgc tacgtagtct
    25801 gtttgagtgg cagcaataaa tccgattttt ttatgtcctt gttcaccatg atgagcgatc
    25861 acttttgcta cactttctcg atcattcact aatattgtat caatacctgc tggtccttct
    25921 ggacaattca atgcgataac tggaatactc atttttgtga gcatttcaaa tccagaccac
    25981 tcttcttcgg ataagaagac aatcagccca tcgatcacat gcttcatcag gtattcaatt
    26041 ttttgttcaa ttttttctgg ctgatcggca aacccgctta ataataagct atacccattt
    26101 tcttcagcta ctctttcgat ccctgagaca acttccgacc caaaacggct ggaaatcccg
    26161 tttatcaaaa gtccaataga gaagcttcga ttattcttca accctttagc aatgatgttt
    26221 tctttatagt ccaacgctga aatcgcttct gctatttttt tcatgttctt ttccttcagt
    26281 tgctgaccat tcaagtaacg ggaaactgtt cccactgaaa caccagccat cttagccaca
    26341 tcacggatcg atgccataca ttctcactcc tgccagttac atttgatttc tctttcttgt
    26401 tgttcgatta tgatgaccgc attctttgca tagtgttgct tgtcatcaat atccatataa
    26461 ctaaagtctt cagtagagca acttcctctc actattaatt atacttaaac aagtatttga
    26521 accggttcat ttttaatata gcattttttt aaaagattgc aacacttttt taaaacgctt
    26581 tctgaaagtg gcggattttt tgccttttta aaattttatt ctaaaattta ctctatttgt
    26641 gggtgttttg tgcgtttttc tcttccatcc gtttgaagtt ttacattcca atggtataat
    26701 ttctttatcc tatcaaatgg gggaagtatt ttgaagaaat taatcgtact atttattttg
    26761 tctgcgggac ttttgacatt gactggctgt gatttgtttg gcggctctga ccaatcctcg
    26821 gacgaaacaa ccgattcttc tgtcttaaaa gcggaaaaag cagataccac caacagtgta
    26881 tatacgctaa aagtacgtaa agatatggat ttatccgcgg gacagttgac atttacagat
    26941 aaaaaaatgg aatggacacg cacgtatcag acacaaggaa ctgattcttc tgaatcatct
    27001 gaagaaaaaa cagtgctgac agatatcaag atcactacca aagatgacga atatattatt
    27061 tcaggaaaag aaaataaaaa agaaacaaca gtcatattta caaaaatcgg taattaccgt
    27121 ttgaaggatg aggatggcaa catctacagt ctatagtgcc gtctgttgga tcttcttttg
    27181 tttctcccct tcttttctgc tatagtaaag aagcaagatg attgtaaagg agacgaaatg
    27241 gatgatcgat agtcctgatt attgtacatg taataacaac aaaaatatcc atacagaatt
    27301 taatgaccaa ggaagcttga gcatatgcga caactgtcac aaactagtca aagattcatt
    27361 taaaacattt gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa
    27421 tccaagcaaa actcactatg gctgattttt atgaacttca aactcagctt cttcctttga
    27481 tcaagcttca tcagtaaaac aaacagtgga tcatttcagt ttacaaatca aagagtaaag
    27541 atttatttat ttcaaatatt tgaatataaa ataaccaaac gatagaaagg cttattctac
    27601 atcacgtagt aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac
    27661 cttttgcatg ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta
    27721 atttttcttg gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga
    27781 atttatcccc atccacttgt attttaggac atacatcaaa atcttcatac cattgattat
    27841 agcgggtgtt caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat
    27901 agtcacgccc acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga
    27961 ccatcaaatc aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta
    28021 ataagctatc gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat
    28081 ggaacaacag gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag
    28141 catttttcat tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact
    28201 gctcaggatt tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg
    28261 gtttagaatc aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg
    28321 ttcctgaaat caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt
    28381 aagtttcaac aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat
    28441 acgtatggag actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga
    28501 tcatcggttt tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg
    28561 tttgatgctc aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat
    28621 ctttttcgta tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt
    28681 gatgacgtgg agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac
    28741 gacttactcg atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca
    28801 ttattttgcc tccttcaata ataactgatt tttaatagat actgctcgta attcattgac
    28861 cagttccaat ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc
    28921 ctcttttgca aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa
    28981 tattccgatt ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa
    29041 ttcctccaat gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa
    29101 taataccttt ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg
    29161 caattcagtt tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag
    29221 tactgtttta ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat
    29281 ttcttcttgt gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt
    29341 accaaaacgg gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa
    29401 gtctatcttc attctacacg tgttttttta aaata
//
LOCUS       DASJTC010000026        29100 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.026, whole genome shotgun sequence.
ACCESSION   DASJTC010000026 DASJTC010000000
VERSION     DASJTC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.026"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1031)
                     /locus_tag="U4B19_001034"
     CDS             complement(<1..1031)
                     /locus_tag="U4B19_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2076791.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSVVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="U4B19_001035"
     CDS             complement(1113..1802)
                     /locus_tag="U4B19_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2076792.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="U4B19_001036"
     CDS             complement(1908..2276)
                     /gene="rplL"
                     /locus_tag="U4B19_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEN2076793.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2329..2832)
                     /locus_tag="U4B19_001037"
     CDS             complement(2329..2832)
                     /locus_tag="U4B19_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEN2076794.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3036..3163)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="U4B19_001038"
     CDS             complement(3242..3931)
                     /gene="rplA"
                     /locus_tag="U4B19_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEN2076795.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="U4B19_001039"
     CDS             complement(4035..4457)
                     /gene="rplK"
                     /locus_tag="U4B19_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEN2076796.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4800..5468
                     /gene="sdaAB"
                     /locus_tag="U4B19_001040"
     CDS             4800..5468
                     /gene="sdaAB"
                     /locus_tag="U4B19_001040"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEN2076797.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5481..6353
                     /gene="sdaAA"
                     /locus_tag="U4B19_001041"
     CDS             5481..6353
                     /gene="sdaAA"
                     /locus_tag="U4B19_001041"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEN2076798.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6444..7463)
                     /locus_tag="U4B19_001042"
     CDS             complement(6444..7463)
                     /locus_tag="U4B19_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEN2076799.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7636..8541)
                     /locus_tag="U4B19_001043"
     CDS             complement(7636..8541)
                     /locus_tag="U4B19_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2076800.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="U4B19_001044"
     CDS             complement(8600..9145)
                     /gene="nusG"
                     /locus_tag="U4B19_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEN2076801.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9246..9416)
                     /gene="secE"
                     /locus_tag="U4B19_001045"
     CDS             complement(9246..9416)
                     /gene="secE"
                     /locus_tag="U4B19_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEN2076802.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="U4B19_001046"
     CDS             complement(9440..9592)
                     /gene="rpmG"
                     /locus_tag="U4B19_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2076803.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9754..10398
                     /locus_tag="U4B19_001047"
     CDS             9754..10398
                     /locus_tag="U4B19_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2076804.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10454..10957)
                     /locus_tag="U4B19_001048"
     CDS             complement(10454..10957)
                     /locus_tag="U4B19_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN2076805.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10970..11016)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11554..12519
                     /locus_tag="U4B19_001049"
     CDS             11554..12519
                     /locus_tag="U4B19_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN2076806.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12618..13790)
                     /locus_tag="U4B19_001050"
     CDS             complement(12618..13790)
                     /locus_tag="U4B19_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2076807.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13777..14922)
                     /locus_tag="U4B19_001051"
     CDS             complement(13777..14922)
                     /locus_tag="U4B19_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2076808.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14936..15868)
                     /locus_tag="U4B19_001052"
     CDS             complement(14936..15868)
                     /locus_tag="U4B19_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2076809.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15985..17064
                     /locus_tag="U4B19_001053"
     CDS             15985..17064
                     /locus_tag="U4B19_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2076810.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17061..17679
                     /locus_tag="U4B19_001054"
                     /pseudo
     CDS             17061..17679
                     /locus_tag="U4B19_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17789..18016)
                     /locus_tag="U4B19_001055"
     CDS             complement(17789..18016)
                     /locus_tag="U4B19_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2076811.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18119..20707)
                     /locus_tag="U4B19_001056"
     CDS             complement(18119..20707)
                     /locus_tag="U4B19_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEN2076812.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20727..21386)
                     /locus_tag="U4B19_001057"
     CDS             complement(20727..21386)
                     /locus_tag="U4B19_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2076813.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="U4B19_001058"
     CDS             complement(21435..21938)
                     /gene="trmL"
                     /locus_tag="U4B19_001058"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEN2076814.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22159..23025)
                     /locus_tag="U4B19_001059"
     CDS             complement(22159..23025)
                     /locus_tag="U4B19_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2076815.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23134..23775
                     /locus_tag="U4B19_001060"
     CDS             23134..23775
                     /locus_tag="U4B19_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEN2076816.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="U4B19_001061"
     CDS             complement(23837..25198)
                     /gene="mgtE"
                     /locus_tag="U4B19_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEN2076817.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25216..26115)
                     /locus_tag="U4B19_001062"
     CDS             complement(25216..26115)
                     /locus_tag="U4B19_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEN2076818.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26117..26914)
                     /locus_tag="U4B19_001063"
     CDS             complement(26117..26914)
                     /locus_tag="U4B19_001063"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEN2076819.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26892..27596)
                     /locus_tag="U4B19_001064"
     CDS             complement(26892..27596)
                     /locus_tag="U4B19_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEN2076820.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27727..28302
                     /locus_tag="U4B19_001065"
     CDS             27727..28302
                     /locus_tag="U4B19_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEN2076821.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28420..29097
                     /locus_tag="U4B19_001066"
     CDS             28420..29097
                     /locus_tag="U4B19_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEN2076822.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8564 a         6167 c         5230 g         9139 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactac acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa tctatattca ttaaataata gtttaacata aaaacaaaaa
     1861 agcaccgaaa tccaacatag gatctcagtg cttttaaata gtaaaaatta tttaacagtt
     1921 acagatgcgc caacttcttc aagtttagct ttcaattctt cagcttcttc tttagatacg
     1981 ccttctttaa ctggtgcagg agcgccatca actacagctt tagcttcttt caagcctaaa
     2041 ccagttgctt cacgaactgc tttgataact ttaacttttt ggtctccagc tgcagttaat
     2101 tctacagtga attctgtttg ttcttcagca gcagcagcgc caccagcagc agctacagct
     2161 acaggagcag cagcagatac gccaaattct tcttcaatag ctttaactag gtcgttaagt
     2221 tctaggatag ttgcttcttt taactcagca acgatgtttt caatgttcaa tgccattata
     2281 atttcctccg tttaattgtt cttttttatt taatgcttta tgcggcaatt aagctgcatc
     2341 tccatcttct ttgtctgcca ctgctttgac agcgtaagcc acgttgcgga ctggcgcttg
     2401 tagtacagat agcaacatag atagaagtcc ttcgcgattt ggcaattttg ccaatgctgt
     2461 gatttcttcc aatgaagaga ctttaccttc gatgataccg cctttgattt ctaatgcttt
     2521 tgcttcttta gcaaattcgt ccatgatttt cgctggtgcg actacatctt cgttactgaa
     2581 tgcaacagct gtaggtccag tgaatacttc gtccatgcct tcaaggccag cttttttagc
     2641 tgcacgtgaa agaatagaat ttttgataac tttcatttct acgttagcat cacgaagttg
     2701 tttacgtaaa cgtgttactt catcaactgt taatccgcgg tagtcaacga ctactactga
     2761 tgcagctgct gtaaattttt cgcttacttc atcaacgatt gctgcttttt ttgcgataat
     2821 tgcttcactc atttttgagt ttcacctcct gaattttgat ggaagagttt tttgcactga
     2881 aaaggcaagt cacttttata gttactttgc tcaaccagta ttattcatca cctattcact
     2941 tcgttcatag tgattctact cgctttgctc gaaaggaaac tcctttcgct ttgctcaacc
     3001 agtattgttc atcatctctt cacttcgttc atagtgattc acaataaaaa actctatgcc
     3061 accgcagaca tagagggact attgaatata ttcaataaac gtcctcggta ggaaattaag
     3121 gcttgatgcc acctactgtc ttcggtacaa gttaattatt aacctcaact agcttagcat
     3181 gctaaactag ttgagtcaag aattattttt cagttggatc aatcaacatt ctaatattta
     3241 attagaaaga agcttggtca acatggatac caggtccaaa tgttgttgtg atcgttacat
     3301 ttttcatgta ttgaccttta gctgctgatg gtttagcttt tagcaataca tcatggattg
     3361 ctgcaaagtt ttcaactaat ttttcatcgt caaatgaaac ttttccgatt ggtacatgga
     3421 tatttccagc tttgtccaca cggtaagtta ctttaccagc tttcacttcg tttactgctt
     3481 ttgttacgtc cattgtaact gtaccagttt tagggtttgg cattaagcct tttggtccta
     3541 atactcgtcc tagacgacct acagtagcca tcatatcagg tgttgcaaca actacatcaa
     3601 agtcaaacca tccgttttgg attttttgaa ccatgtcgtc gtcacctacg aaatctgcac
     3661 cagctgcttc agcttctttt gctttgtctc ctttagcaaa aactaaaaca gtttgtgttt
     3721 taccagttcc gtttggtaat acaacagcac cacggatttg ttggtctgct tttttagggt
     3781 ctacgttcaa acggtatgca acttcaactg ttgcatcaaa ttttgcaatg tttgtttctt
     3841 ttgctaaagc tactgcttcg gcaacaggat aaactttact tgaatcaact ttttttaatg
     3901 catcttgcat ttttttgctc tttttagcca ttttggttcc tccttgattg tggttataac
     3961 ggttgtacct cccacgtgtt ctatatcttt caatatagag ccaactgaga agctgctgtc
     4021 ttagggtgta tagattattc tacagtgatc cccatgcttc gtgcagttcc ttcaaccatg
     4081 cgcattgctg cttcaacgtt agctgcgttt aggtcttcca tttttaattc agcgatttct
     4141 cttacttgat cgctagaaac tttcgcaact ttatttttgt ttggttcgcc tgaacctttt
     4201 tcgattttag ctgctttttt caatagaaca gcagctggcg gtgtttttgt aacgaatgtg
     4261 aatgaacggt cttcgtatac agaaataaca accggaatga tcaaacctgc ttgatcagct
     4321 gtacgagcgt tgaattcttt tgtgaatccc atgatattga tacccgcttg acctagtgca
     4381 ggacctactg gtggagctgg agttgcttta cctgcaggaa tttgcaattt aacgattttt
     4441 tctactttct ttgccacgag acatacctcc ttaagtccgt gatgtggtta attggagatg
     4501 catatttctc ctcccacgtt ttatctgatc tgaaaaaatc cagacaaata atgtgcaaaa
     4561 caatgcacac tggtacagta tagcaaattt atttgttttt tcaagatttt tttctgctat
     4621 ttttacattt tgcttgaata aacgctaaat ttattctaaa aaaacaacaa tttttcgttc
     4681 tcatttcttc ttttatgtta cacttgcgct agcatggttc aacagatgaa agcaaacaat
     4741 aacacggttt tttagaccct tgtacaaaga ttcctacata acaaaatgag gtggagaaaa
     4801 tgaaagattt aaggtataga agtgtctttg atattattgg acctgtgatg attggtccaa
     4861 gtagctctca cactgccgga gctgctagaa tcgggaaaat catacgtagt atatttggcg
     4921 aacagcctga ttctgtagat atttatctat atgaatcatt tgcaaaaacc tatcgggggc
     4981 acggaacaga tattgcatta gttggcggcc tgttagggat ggaacccgat gatgaacgtt
     5041 tagctgattc tttgaaattg gcatatgaag ccggtatgga agtcttgttt gttcctaaga
     5101 gtgaaaaagc cgaacatcca aactctgtaa agatgcttct aaaaaaagga acacgaaagc
     5161 tttcagtcac tggcatttcg atcggcggtg gcaatatcca gatttctgaa ttgaatggat
     5221 tcaaaatttc tttaagcatg ggtactccta ccttgatcgt tgtccaccaa gatgttccag
     5281 gtatgattgc taaagtgact agtatacttt ctgagaccaa tacgaatatc ggcactatga
     5341 cagtgacacg ggaatcaaaa ggtgaaaatg ccattatgat aattgaagta gatgatcctc
     5401 aagtagaaga aacggtaaaa agactcaaac agctgccaaa tatcgacagc gtaaattatt
     5461 tcgattaagg agctaaaaaa atgtttttat cgattgaaga attagtagat caagcaaaag
     5521 cttatccaag tgtagcagaa ttgatgattg cagttgaaat ggaaatgagt ggccgttccc
     5581 gtagtcacat catcgagatc atggaaaaaa acctctcagt aatggaacaa tcaatcaacg
     5641 agggaaaaga aggtgttacc tccgtcacag gaattaccgg tggtgatgca aaaaaattag
     5701 atgattattt aaatgaggca gactttttaa gtggagaaac aatcttaaaa gctgtacgca
     5761 atgctgtagc tgttaatgaa gtcaatgcaa aaatggggtt gatttgtgct acaccgactg
     5821 ccggaagcgc gggtgttgtt cctggtgtgc tgatggctgt ccgagatcgg cttgatttga
     5881 cacatgaaca gcaattggaa ttcttattta ccgctggcgc tttcgggtta gtcatcgcaa
     5941 acaacgcttc tatcagtggt gcagaagggg gatgtcaagc agaagttggt tctgcaagtg
     6001 caatggcagc agcagctttg gtttgtgcaa aaaatgggac tcctcatcaa gcagcacaag
     6061 cagtagccat tacattgaaa aatatgatgg gactgatttg cgatcccgtt gcaggcttag
     6121 ttgaagtccc gtgtgtcaaa cgtaatgctt taggctcttc tcaagctttt atctctgctg
     6181 atatggcatt agcgggtatc gaaagtgtca ttccgccgga tgaagtcgtt gctgcaatgt
     6241 accaagtcgg acgtcaaatg ccgtcgattt tcaaagaaac agcagaaggt ggcttagctg
     6301 ttacgccaac tgctaaacga cttgcggaag agattttaaa taagagggcg tgataaagct
     6361 ccttaatttc gagtattaag agattagtta tcttcataac aaaaaacgaa gagaggacta
     6421 attgcccttc tcttcgtttt ttattaatcc atcaatttcg tattttttac ttcatcgatc
     6481 gtttttgttc ctgcaagttg catcgtgatc gacaattctt tgttcaggtg ttcaaaaaca
     6541 gatgttactc cttctgcccc acctagattc aacccataaa tcactggtcg gccaatcgca
     6601 actaagtcag caccacttgc cagagctttg aagacatgct ctcctcgacg gacaccagag
     6661 tcgaaaatca ctggtacacg tttgttgact acttcggcaa tttttggcaa tacttcaaag
     6721 gacgcaggac ctccatctag ttgacgtcca ccatgattgg atacccagat accatctgcc
     6781 cctgcagaga tagcgattac tgcatcttct ggcgattgta ttccttttac gataactgga
     6841 agatgggtga tttctttgat tgttttgatg tcatctggtg tcaagccttg ttttgcagct
     6901 gcataaattt ctgcgattcc ttttccttcg ccattaccac ttgcactttg ttcgctatac
     6961 gcagctaagt taggcattgg taatggaaat tggaattgat tgatgacgtc ttcttcacgg
     7021 taaccaccga gcgttgaatc tgccgttaaa atgatagctt tagcccctgc ttctactgct
     7081 ttgttcaaaa tgaactcatt gaatctgtcg tctttactca tatataattg gaaaaactgc
     7141 ggtgcgcctg gcgctgcatc ggctgcgtct ttgattgtcg tatttgcata agtgctgatg
     7201 gaaaagattg atccagcagc agctactcct ttagctgtat cagcttcccc tttttcatgt
     7261 gccaatcctt gggccgctga tggcgcttgg ataatcggtg tatctaattc aattccgaaa
     7321 acagatgtat gcaaatcggc tgagtcgatt ccgcgtaata tccgtggcat gattttcttt
     7381 gtattaaaag aagtcgtatt ttctttcatc gtccactcat cttcagaacc accgcgaata
     7441 tagccaaaag ctcctgcttc catgcgctct tttacacgac cttctagtta ggcaatgttt
     7501 acaatatcga ttggatgttc ttcattactt gtatgataag tcatagtaga tccttctttc
     7561 atcttcttga ttacatataa atcatactaa acttacaaaa agtaagcaaa catttactct
     7621 cagatacttt tatcgctatt ctttccaaat aaatcgactt acaagctgat ctacttcttg
     7681 gttttcatga agcatactat gagaggcatt ttcacctgtc actacttttt cctctatttc
     7741 atttccgcgt tgcttcaaca acgcatagac cgctaatgca ctgtttaacg gtactgttcc
     7801 atcactcaag tctgtttcat ctagttgacc agcaatcaag aaaaatcgag tggttatagg
     7861 aatattaccg atcatttgct ggtaatcttg atagcggtta gaaacgacag caggcccctt
     7921 tgctaactca tctgtcagag actgcgcact atcatcgaca aaatcattaa aaggcgcgcc
     7981 aattgctgtt aatgtctgaa cttgtggcaa tgacgtatcc tgtccatacg ttcctaaata
     8041 gcgtagtgca ctaactcctc ccatggaatg ccccactatg ttgactttat ctatgccgta
     8101 gttttctttc agatacaaaa gacaagtttt gatccattct gcttgattcc actcgttatt
     8161 tttattatct tcaaacaata cttggaccat cggattgtct gcttttccag acaaatctcc
     8221 tgttgcttgt atttgtccat ctgttcctac tgttaaaacc aattctttta ctgcttgctg
     8281 atttttttcc aaacgccgca acatcccttt aaaggaattt tctgtcccgc tgtagccgtg
     8341 gaaaaaaagt gttggaatag tggttttttc tatcacttta ctggattctg ccgttttttc
     8401 tttatcagaa acttcagtag tatttatctt cgtcgtctgg caaccactta acgcaaacaa
     8461 actgataagg actaaagctg ctgcagttgc acgtttgcat aaatttttga ttggttctgt
     8521 catacacctc ttcctttcca tatagaaaaa agaaagactt gaaaggccaa tcatatgatc
     8581 gacatttcaa gtctctttat taatcgattt tatctacttg atcgaaatca agttctgtac
     8641 tggtttcacg tccaaacata tcgatgttga ctttcaattt ttctttttct ccgtcaactt
     8701 ctgttacttg tccttctaat ccagcaaatg cgccttcgat gattttcacg acttcgccaa
     8761 cttctacttc tagatcattt tgtcgcaggc tcataccgat agaacgcaag atatgattga
     8821 cttcttctgg aagtaatggt gctggtttgc ttccggctcc gtgtgaacca acgaatcctg
     8881 tcacaccagg tgtgttgcgg acaacatacc atgaagcgtc tgtcatgatc atttcaacaa
     8941 gtacgtatcc tgggaatgtt ttatgaacga tttctttttc tttcccattt ttgacttcag
     9001 tttctttttc ttcaggaaca acgacacgga aaataaagtc ggccattttc atactttgcg
     9061 cacgagattc gatatttgct tttactttgt tttcatatcc tgaatatgta tgcaagacat
     9121 accagttttt ttcaaaagat tccattgaag gctcctcatt tcattgtaaa ataaaaaaac
     9181 ctcagtacac ccgaagtttt tctttcgctt cattatagca ctaaaagtac gttgtggcta
     9241 gcgagttatt ttaaaatcca gccaaataag ctttggatgg ctgtatccat tacgtaaaac
     9301 atcaatccga aaataataga agtttcgatg actactaacg tatctttacg gagttgtttt
     9361 ctgcttggcc aagttacttg tttgatttca tcttttacgc tttttagaaa tttcatgaga
     9421 caacctccct tgctactgtt tatttcgttt ctttatgtaa tgtatattga ttacaatatt
     9481 tacaaaactt attgatttcc aaacgttcgc cgctttttcc ttcactgact gattttgtgt
     9541 agttgcgaga cccacaaacg gaacaagcga gtgctgcttt tttctttgcc atttacttct
     9601 gcccccttgc tgagaatcag acttatactt cataacagta tcacgtaact ttttacttgt
     9661 caatttgtga aataaactat ctattgtttt ggataaaatt atgatatgat taatttgaaa
     9721 ttaagctaaa atgagacaat tggaggtata gcgatgctat taaaaacact tgtatataag
     9781 aaaaaagatc tgacaaccgt tacagagact agtacattag aagaagcatt aaaaatttta
     9841 gaaacttccg gataccgttg tgtgccgatc ttagattctt caggtaatat tttccgtgga
     9901 aacatttaca aaatgcatat ctatcgtcat aaagcaaatg gcggtgatat gacgcttcct
     9961 gtcactcatc ttttgaaaaa cgctacaaag ttcatttacc tggatacttc tttcttcaaa
    10021 gtattcttta cgatcaaaga gcttccttat attgctgtcc ttgacagtga aaatcatttt
    10081 tatggtattt tgacacatag tacgttgctt aatatgctct ctcaatcttg gagcgtagaa
    10141 caaggaagct atgtactgac aattgcttcc acaggaaagc aaggagattt ggctgctatt
    10201 gcaaaaatca tttcaaaaca cagcagcatt gccagctgta ttactcttga tatcgaaaaa
    10261 gacgaataca ttcgacgtac attgatcacg ctaccggcta acactgaagc gaaagtatgt
    10321 gaaacgatcg tcaaccactt agagaaaaag aattataaag tggtagaaat tgaaaatttg
    10381 caaaaccaag aagcataatc aaaaagcgaa aaaagaaaac agctaatctg ctttcttttt
    10441 tcgcttttta taattagaca cgcttttcaa aatcaagcgc gcgatctaaa taatacatca
    10501 atggtgctga aacagccatg acgataaact cacttaatgc aagcgtgcca taggttgtcc
    10561 aaaatgcttg gccacctgtt ggaactaaca tataagcaat cagtcccatg ctgatggtaa
    10621 agaagatcgt gttcaatact aaacgcatct ttacattttt cactttgttt tgcaataaag
    10681 ctgtcaggcc taaagaaagc aatgtttggc cgccaccaaa aaccacatcc atgataccaa
    10741 aaccaaagaa ggcattatag acgatgaccc cgcctaaaac tccccaaagc aatttacggt
    10801 taaagacaac taaatgattc aaactttctg aaatacgaaa ctggatcggc cctgaggata
    10861 caggcgcaac taaaatagtt aacgctagat aaagagccat gatgatgcca tttacaacga
    10921 ttacggataa ccgttgtctg cttttcgttt tttgcatttt ctctcctctt ttctcctagt
    10981 ttttttaacg cgggatggtt gatgaaccgc ggttatgtga aacaaacaat aattataaca
    11041 gacttttcat ttatttcaat ccgtcttatc tgcttttaaa aaagcaaaag cttaaacaat
    11101 gtaaacgttt gctaaaaatc gtgggatatg gtataaagta agtgtaaggg gtcataagaa
    11161 ttcccagtaa agcagacgaa gtaggatctg caagaacaga attgttgtga aacttaaata
    11221 aataaaaaag gatgtgggaa tttgagcaac ctcttactag agaacggtct agcaagcagc
    11281 tgaagatcta agtggacatg ccgaaccttg gaacttctcc ttacgtaagt aatggatcat
    11341 gcgcttgcta ggcttttttg tgtttttcgg tcttcggacc tttttcattt tcctagactt
    11401 ctgctataat aaccaagaat taattttgta acagattttt ttgaaaaaac aagtttcttt
    11461 cattcaaaaa atagaaaaag cttgctaatt attggttaac cagcaaacag gagtattaaa
    11521 atatcttgtt ttattgaata gcaggaggaa atcatgaaat tccattatca tatattgatc
    11581 aatccatcgg caggcagcgg aaatggctat aaagtagcag aaagaatttt accggtatta
    11641 aaaaataagc atattgacta tacacttcat tatagtgaat acaaaggaca cgatgctgag
    11701 attgctgaaa ctttggcaaa agaaacattg attccttgga tagaagaaga acagaaaaca
    11761 atcgatactt tccccctttt gatcatcgta ggaggtgacg gcaccttaca tcaggtactt
    11821 gatactttct atcagatgga agtagaattc ccagttgctt atattccagc aggttctgga
    11881 aatgattttg ctcgaggggc aggccttcca aaaaatccca aaaaaggact tcagctgatt
    11941 ttagcagctc aatcaccaga aaaagtccac ataatggctt atgaagaaaa aataagtgaa
    12001 aaaaaaggaa tagctgttaa taatttcggt ataggattag atgcagctat cgtccatgcg
    12061 acaaatcatt cttccacaaa aaagcgattg aacaaataca acttaggatc attttcgtat
    12121 ttattctcta ttctccgtgc tttattcact caaaaaggat ttccaattct ggtagatgca
    12181 ggaggaaaac aattaagttt ttcaaacgca tttttatgta cagcaacgaa gcatcctttt
    12241 tttggcggag gaatcgcgat tgttccaact gctgatgcgt ctaaaccagt cattgatttt
    12301 gtcgtggtcg aacgaatcaa tttctttaaa atcttgtggc tcattctttt acttatacag
    12361 aaaaaacaat tgaaatcgaa atatttccat cactacacaa caaacagatt acgtatcgtt
    12421 tcaacaacac cgcaacatgg tcaagaagat ggagaagaaa tggaaaaacg accttttgat
    12481 atcacaataa ccacaactaa acagttgttt tggtgttaat aaaagaggtt ttgtcagaag
    12541 ctacatctgg aacaccgttt attcggaaaa aaggactgtg acacgacttt tgtcacagtc
    12601 ctttttttac tatgcagtta attctttgcc caattgggtt gccatcgata gtttctttct
    12661 ttacccaaaa taaattggat cattgaaaac accgcagcaa aagcaactaa gacacaacca
    12721 ttgaaccaaa aagattctgc aaatgtttga ttgtaactga gtgtttcact gatcaagtta
    12781 ttattttgaa caaaccaata tgttggtgtg aatgccgcta ttttgctaac catttctggc
    12841 aagtattctg ctggtacaaa caccccaccg ataaagcaac tgcctgtgat aaaaatatct
    12901 ttgatcccac cgctggtttc tgagtttttt accaatgaag tgatacaagc cgaaaagcta
    12961 atgatcggca ataaaaacaa gaaagtattt aaaatatgat accataccgt tatatcccac
    13021 gaggtgtgga tcactaacag aaccattaga agtaagaagc cactgaacaa cagcagcgag
    13081 taactaatca aactaaaggt tactttgcga ttcaagtttc gtttggtaat gggcgcactc
    13141 tggtttcgtt tactgatctc tttgcgatta aacgctagat tgatgacgct gaaaccgcta
    13201 aaaatggata gaaacaaccc ataagacaag aaattaaagg ttgtgcccgt caacagcttt
    13261 ttcatccgct gactatatcc cgcacttagc tcaacttctc cagtaaccga gagtgccatt
    13321 tgtgtctgtt ccatagctgc atcgattgtt ttatcttctt cttgaaacag aatcagtgtt
    13381 tgcaaaaagg tgttcacttg ctgtgtaaca actgttttag aaaaggcatc tgggcgactc
    13441 tgtatcttga tcgtcgccat ctttcctact tcgacttgtt cggtaaagtc tgcaggcaga
    13501 tgtaagataa tgtttacacg attaaaataa agagcatcat cgatttcttt ttgtgaggtt
    13561 ccatccaatg taacgactgt ttccttgtca ttcaaatatt tacgaaggcc atcgactaca
    13621 ggcgactctt cttcactaat caaggcgatt ttcgcatctt gcagagaggt attttcaaca
    13681 tcaatatttt gcgcaaaaaa gaatgtggca acaacagtga taattacacc tatcagtaaa
    13741 ttcgatttat ttgctttaag caatgtccaa atcgttttat aaagcgtcat aacgcacccc
    13801 tctttccatc caagcatttc ctaacgcaaa aaggaaaata aatccgatca accatagaag
    13861 attggtataa aaactagcca aatcagtgta gaaataaagt ttatacaaac tttcgctgat
    13921 caagttgacg agatttaatt gaccaacaat cggaatatga agatcaatcc aatacttcac
    13981 ttgttccgac cccatcatac cagcaaaaaa gctcatcgca atcgtcactg aaatcaagat
    14041 gctatccttt tgcaccagat tcatttttgg tatcaaatta ccaaccaatg tacctaacaa
    14101 aatcgcacac aacgacccca gagcggtaac aatcatcaac cattgccaac gttcaccaaa
    14161 gtctacttga tagataaaac gaaagacagc taacataata taaatctctg tcaaaacaat
    14221 cgtaaagctt gctgccaaat tagcgattga aactaacgat tgctttctag gaatcaagga
    14281 caagcgaata ccatttgacg attgatttgc ttgctgatcc tgtacattgc ggacccccca
    14341 catcgtcccg tagagaatcg ccattccaat cagagtgaag aaataaaaac ttttgaaact
    14401 catattacga cctgcatgaa cttcttgaaa agatacttct tgttcaaaaa cagtcatgat
    14461 gggcgcgaga tcaacgttct ccacttctgc cactttactc gaaattatac caatacgctg
    14521 taaaaattga tccatgacgt ttttcatcaa cgtctgactc attcctgctt gacccacatg
    14581 gagcgaaaca gatgaaccat caatgagata aaaaccagcc aaatcatctg catttagctt
    14641 ttcattcgct tctacttccg atagttcctg tacttcaaat aatggtttat cattgacttc
    14701 gacttgttct aagactgtgg taaaattttc cgaaagcgct gtttgatccg ctttgattac
    14761 aattccagta ttaatcgttt caagattgcc accttgatca atctcgccaa aggccgccat
    14821 aaacatcaat cctaaaagaa ttggaaaggc caatgtccaa aacaacatgg atttatttcg
    14881 cagcagtatt tttgtgcgat atttataaaa atgccaaaac attatttccc ctcctctagt
    14941 ctctcaaatc cttgccagta atctctaaaa agacatcatt caaggacgct tctttggttg
    15001 ctatttttcc gtaactgatc ttttcttctt gtaagattgt aagaatcggc aacaatactg
    15061 tatttagttc agtagttgtt acttggagct gacctttttt ataagcgatt ccatcagaag
    15121 ctattgttcg ctgaatcgct gacaacacat tttctgggag taatgggatg tcgatcgtca
    15181 aacgatccgc cgtccgtacc atatctttta gagattcttt ggttccttta gcaatcacct
    15241 gtccttgatc catgatcacg atctggtcgc aaagaatttc tacttcttcc atgtaatgtg
    15301 ttgtatagac aatggttgct ccttgtcgat tcagctcctt gatactttct aaaattttat
    15361 tccgactttg ggggtctaca gctactgtcg gctcatctaa aaaaattaac cgcggcttgt
    15421 gggcaatccc acatgcgatg ttcaaccgac gtttcaagcc accactaagt tctcctggac
    15481 gaaacgaaac atatttttct aatcccacta attgaatcac ctcttcaatc aaggctttcc
    15541 ttttctcttt ttcggaaaca tataaaccgc aaaaataatc gatattttct tgcacactta
    15601 gttcgtcaaa gaccgcaacg tcttgaggaa cgaccccaat cattgccttg acagcataat
    15661 tttcaggagt cattgttgtc ccgaacaatt taatggttcc tttgtcataa tttaacaaag
    15721 aaagcataca gttgatggca gtcgatttcc cactgccgtt tggacctaac agacctaaga
    15781 cttctccttc ttgtacttgc agatcaaaat gattcaatgc tacaaaatct ccatagcgtt
    15841 tgactaattt atcaatttcg ataatcatca tgaaaaactc cttccaaatt tcttgttgat
    15901 ctgactataa taaagtgcaa gaaaagaaac tagtgacgat tggctaaacc acacatgaca
    15961 tttgtcacaa tgaaggagtc tgctatgcat aaatctcggc tactcaacga aactagcttt
    16021 ctactggtta tctcattgct tttgctggta agtcaaggat tcgttgctat gcaagttatc
    16081 tatttattga tcgccgtttt attcactggt gtcagttatt tactgcctac aaagccaaat
    16141 tggattttcc aagttattct atttctttta tgtgcctttt tacctgatct tgtcctgttt
    16201 ctgccagcga cgatgcgagt gatcgcccct gaagaaagcc accttgatta cttgtttttg
    16261 ttaggttttt tgttgatttc gacggttggt aatcatcccc ccatcaaggt cgcctttgtc
    16321 gtaattttgc tgtttttttc cttttactta cgaaaaaaag atcaacgaac gaccgatctt
    16381 gagaaaaagt acattgtctc caaagatact cattgggaaa aacaacaaga gttatcggaa
    16441 caaaataaag cattggccct gtcacaagaa acacaattag ccttgcaaat tgtggaagaa
    16501 cggaaccgaa ttgcccgaga catccatgac aatgtcggtc atctgctgtc aagtgcgatt
    16561 ttacaaattg gtgcgcttga aatgatcaat caagaaccaa aactaaaaga accattagaa
    16621 aatttaagtg ccaccgttca cactgggatg gatcggattc gtcaaagtgt ccatgacctc
    16681 catgagacat cgatctcttt tcaacactcc ttagagtttc tgatcgctga ttttccttct
    16741 tctgttacga ttgaaggaaa cttgtttgaa ggattgaatg aaacgcagca aaactgcttc
    16801 ttaatggtca tcaaagaagc cttaaccaac agtatcaaac atagtcatgc caaaaccgtt
    16861 gttttacatt ttcgaacgtt gccggccttt tatcgcttgc aaatcacgaa tgatggcacc
    16921 agcccacaaa aaaattctgc tcccggtatt ggtttgaaaa cgatgcgtca acgtgttcaa
    16981 gaacaaaagg gacaagtcca tattttccaa gaaaaccagc aatttcagtt aacgatcatt
    17041 ttaccaaggg agggacacca atgataacag tagtcattat tgatgatgat ccatttgtga
    17101 cgaaatcgtt acaaaccatt ttagaaagca cgaaagagat ccgtgtgtta ggcattggtc
    17161 attgtgccaa ggatgcgcga aacccatcgt cccgatgttt tgctgacgga catccgcatg
    17221 cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc caaagccatc
    17281 cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt ttccatcggt
    17341 gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt caaatccgct
    17401 tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca aatgatgaag
    17461 aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga gcttgcaatc
    17521 atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt gtacttgagt
    17581 gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt acgggatcgc
    17641 acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa cacaaaaaaa
    17701 cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt caattgtccc
    17761 gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca ggattcgttg
    17821 gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt tctagttttg
    17881 attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat tctttttcca
    17941 tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct aatttatctt
    18001 cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca tatcacactg
    18061 aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat ctttaaaatc
    18121 aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca atcatcggat
    18181 ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa acagcaggtt
    18241 ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt tccactgttt
    18301 ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt agcttttctt
    18361 ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt ccgaccaaag
    18421 aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca ttatccatga
    18481 tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa gtgacatagg
    18541 cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc gtgtagagca
    18601 aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc atcttgaatt
    18661 cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta ttccattcgt
    18721 ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc actttatctt
    18781 cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg aatacattca
    18841 attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg gtgtcgttcc
    18901 aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc attttattta
    18961 acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata tcttgtgcac
    19021 ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca atttgataag
    19081 catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt ggcagacgtc
    19141 cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc cgataaatct
    19201 gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt acttgaccag
    19261 gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata ttcgtcggaa
    19321 tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca tcaacaatca
    19381 aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt ccattcagcc
    19441 ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt cgtttagctg
    19501 ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat tttgaaggat
    19561 ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc acagtagttt
    19621 tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct tcagcgatag
    19681 cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc cgaatattct
    19741 tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg cgctggatcg
    19801 aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta gtctcttctt
    19861 gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga tcaggctgga
    19921 tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat gtctccgccg
    19981 gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag atagctgctc
    20041 gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta tctgcacgtt
    20101 tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg atgatatcca
    20161 aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg aacccgtaac
    20221 gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc tcagcaagca
    20281 tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga tcagcaatga
    20341 ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt ttcttcccga
    20401 ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt gttggctttt
    20461 cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc actaattctc
    20521 cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg actacaatct
    20581 ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact ttgtaaaaat
    20641 tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc atttcttctg
    20701 gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt ttcagttcct
    20761 aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca aggctcttca
    20821 gtctctaaaa tcgtcaaact actatttaac aatccaccca tttcacgcaa ctcacttagc
    20881 tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc tacgaataaa
    20941 agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat acgtgtgatt
    21001 gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa atgattacgc
    21061 aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg accttctaaa
    21121 gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg acgagcaagc
    21181 tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga ataaattgct
    21241 ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta actttgtggc
    21301 aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca ttgtgtcttc
    21361 ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt ttattttact
    21421 ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa ttctaaatta
    21481 gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt cgataaattc
    21541 aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc gttttcacgc
    21601 ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg ctcttctcca
    21661 tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa atgtagttta
    21721 cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat atttacatca
    21781 ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa acctagtggc
    21841 tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt tccagtgttt
    21901 gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc tctcatattt
    21961 atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt atcaactcgg
    22021 tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt tatcagaaca
    22081 ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc ctcaactttt
    22141 ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag ttccttttaa
    22201 taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg ctttgattcg
    22261 ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat tttcctctgg
    22321 tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt cagcaaactt
    22381 ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat aaaaagcctg
    22441 acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc catcttctga
    22501 gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa ccgcgttgaa
    22561 tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg cttcggcaga
    22621 ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag cattttgaat
    22681 ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa catctaatag
    22741 cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat acatgccaga
    22801 ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt taaccgcatg
    22861 ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat ttttgcatat
    22921 caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga acagctgatg
    22981 attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc cccctcattt
    23041 ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg atacaatgcc
    23101 tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg cgctgaaaac
    23161 tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt gtgtgacaac
    23221 ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt aagttatgct
    23281 ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga cttcgtctat
    23341 aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa attagggacg
    23401 gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga ggccttagaa
    23461 ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt tgacagtatt
    23521 cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt tgaccgtatc
    23581 ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg gcttaaagaa
    23641 ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt cactgcagag
    23701 aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac caaaatcgtg
    23761 gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa tgcttattgc
    23821 aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata ttgaaataaa
    23881 tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct actgctggat
    23941 caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc gccactgtga
    24001 ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt ttccaaaaac
    24061 ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg gtcaagactt
    24121 cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca agtcgacgaa
    24181 cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc agcgaaatga
    24241 agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt aaagaagcgg
    24301 tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac gctgccttta
    24361 ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa tcgctagcag
    24421 cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac aaatgatcat
    24481 cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt gccacatctt
    24541 cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca gaaatcattg
    24601 cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc tgaccttctt
    24661 gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc ttcagcacgt
    24721 acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt gtcatgatcg
    24781 cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca tcgtctgccg
    24841 acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc aagtcaacag
    24901 cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc atttcatcta
    24961 ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc gctaattctt
    25021 taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat tggtagaatt
    25081 gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga atattatttt
    25141 cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt tcatccaaac
    25201 caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt ctgcggctaa
    25261 aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca gatcataaca
    25321 atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat catcaccgag
    25381 cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc ctgtatgcag
    25441 ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact cagtctgtgc
    25501 cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc gggcgatcgg
    25561 caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta aggcctgata
    25621 ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg caaatccatg
    25681 ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca catggattac
    25741 ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca ttgtcccatt
    25801 tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta ccagaacgtg
    25861 ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt catgttctcc
    25921 ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca agacattttc
    25981 tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta acagaccctt
    26041 tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt tctcataatc
    26101 ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga tgccaaaaat
    26161 gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa taaattgagc
    26221 ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct tctgtatttt
    26281 ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc aaagtacgaa
    26341 acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata ctaggatgga
    26401 tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta gaaatcgtca
    26461 atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg gctaccatcg
    26521 tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg tccggtgttt
    26581 catccaaata actgatccga acatccagca agggataact aacactttgt tcccggttcg
    26641 ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg taaaacccta
    26701 aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg tagtgatgga
    26761 acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc tcatgatcat
    26821 ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact tctatagtct
    26881 ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct ttcgctttct
    26941 ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg ggagaataac
    27001 cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa ctgataggca
    27061 gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc ttgatattta
    27121 taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg aatctggatt
    27181 tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat caccagatga
    27241 taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg gagaactttc
    27301 atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac aaactgacac
    27361 atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat tggaatatgc
    27421 cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt gacaaattca
    27481 atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag tttcactttc
    27541 aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg ttccatagct
    27601 gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa gttttttcgt
    27661 actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca aaaaggagtc
    27721 gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa gcaagagtac
    27781 gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca gactaatatt
    27841 tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg gatccgtttc
    27901 gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg tctcgaaaca
    27961 acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg tctgcctcgt
    28021 tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc attaatccaa
    28081 accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact tttagctatt
    28141 gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt aggggatgca
    28201 tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc ttatcgtcct
    28261 gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt cattttcaca
    28321 aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg aaagaatcta
    28381 gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat ctatttattt
    28441 gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca atttatcgaa
    28501 gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc tttggtcaat
    28561 ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa tcttgaggaa
    28621 cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc ggcgcagttt
    28681 caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt cggctgtaat
    28741 gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg cggcgatttg
    28801 aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt ttctgatcaa
    28861 aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta caattatgcc
    28921 tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct ttggagaatc
    28981 acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga acatttacat
    29041 acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc taattaaaaa
//
LOCUS       DASJTC010000027        29019 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.027, whole genome shotgun sequence.
ACCESSION   DASJTC010000027 DASJTC010000000
VERSION     DASJTC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.027"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(296..1024)
                     /locus_tag="U4B19_001067"
     CDS             complement(296..1024)
                     /locus_tag="U4B19_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEN2076823.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="U4B19_001068"
     CDS             complement(1002..2576)
                     /locus_tag="U4B19_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN2076824.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="U4B19_001069"
     CDS             2805..3743
                     /locus_tag="U4B19_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2076825.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="U4B19_001070"
     CDS             3755..4666
                     /locus_tag="U4B19_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2076826.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="U4B19_001071"
     CDS             4690..6162
                     /locus_tag="U4B19_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2076827.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="U4B19_001072"
     CDS             6213..6824
                     /locus_tag="U4B19_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEN2076828.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="U4B19_001073"
     CDS             6854..8767
                     /locus_tag="U4B19_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEN2076829.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="U4B19_001074"
     CDS             8895..11876
                     /locus_tag="U4B19_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HEN2076830.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="U4B19_001075"
     CDS             11873..13534
                     /locus_tag="U4B19_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEN2076831.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="U4B19_001076"
     CDS             13574..14506
                     /locus_tag="U4B19_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEN2076832.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..16445)
                     /locus_tag="U4B19_001077"
     CDS             complement(14898..16445)
                     /locus_tag="U4B19_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEN2076833.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
     gene            complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="U4B19_001078"
     CDS             complement(16547..16900)
                     /gene="tnpB"
                     /locus_tag="U4B19_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEN2076834.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(16890..17084)
                     /locus_tag="U4B19_001079"
     CDS             complement(16890..17084)
                     /locus_tag="U4B19_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076835.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            complement(17310..18512)
                     /locus_tag="U4B19_001080"
     CDS             complement(17310..18512)
                     /locus_tag="U4B19_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2076836.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(18515..18709)
                     /locus_tag="U4B19_001081"
     CDS             complement(18515..18709)
                     /locus_tag="U4B19_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN2076837.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(18756..19604)
                     /locus_tag="U4B19_001082"
     CDS             complement(18756..19604)
                     /locus_tag="U4B19_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEN2076838.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(19809..20159)
                     /locus_tag="U4B19_001083"
     CDS             complement(19809..20159)
                     /locus_tag="U4B19_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076839.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            20754..21200
                     /locus_tag="U4B19_001084"
     CDS             20754..21200
                     /locus_tag="U4B19_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN2076840.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            21824..22816
                     /locus_tag="U4B19_001085"
     CDS             21824..22816
                     /locus_tag="U4B19_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2076841.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            22832..23716
                     /locus_tag="U4B19_001086"
     CDS             22832..23716
                     /locus_tag="U4B19_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2076842.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            23765..25372
                     /locus_tag="U4B19_001087"
     CDS             23765..25372
                     /locus_tag="U4B19_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2076843.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            25373..26857
                     /locus_tag="U4B19_001088"
     CDS             25373..26857
                     /locus_tag="U4B19_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEN2076844.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            27034..28026
                     /locus_tag="U4B19_001089"
     CDS             27034..28026
                     /locus_tag="U4B19_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2076845.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            28078..28947
                     /locus_tag="U4B19_001090"
     CDS             28078..28947
                     /locus_tag="U4B19_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2076846.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
BASE COUNT         9157 a         4760 c         5860 g         9242 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
    16201 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaagattc
    16261 aattgcccat tcgtttcctc aaagagagtc tctttagagc gtccaaataa tttgttggtt
    16321 aaataattga ccttttcatt caatagttgg atttcagttg taagattctg aatggtttgt
    16381 tgttgtgctt gattggtttt atgggcttct tgaagcaact gaagcaacgt atctgtagtt
    16441 tccaaaattt ttcaccacct tgaatcgaac tgattttgtt acagaatacc acaagaaacg
    16501 cacaagaaat aggggttcaa cacatttttt gtgttaaccc cccagcttaa aaaactcctt
    16561 tttttcctgg caaaatagct cgaggttgat cgatacttaa tccttctaac aaccatcgaa
    16621 cctgttggtt cgttaaggtt cgaacttcgt ctttcgtccg aggccatttt aacttgccgt
    16681 tttctatccg cttataacag agaagaaaac catcaccgtc ccaatagaga agtttgaaac
    16741 ggtcttgacg gttcccgcaa aataaaaaaa cggcatcttc gtaaagctcg agttgatact
    16801 cttgttgaat aagactggca agaccatcaa ttccttttcg caaatctgtt tttccgcaca
    16861 caatataaat atgtttggtt tgaagtaaat cataaagcat tggacaacag ttctttcaaa
    16921 atagcttgaa gaatataatt gttaataccg ttatagatca aaacttcaga accattctct
    16981 cttttgatgc gcatcgctaa cttggcagcc gcgggtttac ctttttctgg tgcactcttt
    17041 tcgtttccta attggacagg aacaatctca tttaatttat acatagaaaa cctccagact
    17101 tcttgatagc tcaagtatat cttgaggtgc atttgttagt aaggtactgt tctatggggc
    17161 gcttacgttg aaaaacgttg atttatcggc attccccatc catattatat ttacgtctta
    17221 ttcccactca atatgagtag cctcgtaaag aagctattat atcagtgctt tttcagccta
    17281 ttcctcattt tggtagggaa atggtagggt tagattttta ctgcttgatt tccattaaag
    17341 tttgcaactt tagattttgt tgcacaggta aatttaatgt ttcctgtcat ttgttctgca
    17401 attccttcat cattacggct ccatagatga gcgtagtgct ttaaagtgat ttcagggcta
    17461 gaatggccta accgttgcga cacgaccaga atgtctgcgt taaactcatt gattaggtag
    17521 ctaacatgag aatgacgtaa tccttttcct tgtattaggg gtaccttagc tagtttggca
    17581 taacgctgaa taattcgttg aacagttgaa cgatagagtg ggaggtcagt gtagctaaga
    17641 ataaattgtt ctacaccatg atttttttga ataactttcc acttttttag cacttcaatt
    17701 gtatcatcgt ccagagaaat gatccgcaag ccgctttcag tttttgtgta aggtttccga
    17761 ataaaatctt tttgatttgt catttccaag gtgtgatgaa ccctcaactt tttacgtttg
    17821 aagtccacgt cattccaatt aagtgctaat ccttcgctta cacgaacacc agtcatgtaa
    17881 tatagccaga tcattaaaaa acacatatgt tcgtagaaat catctttata aatttttgcc
    17941 aaaacttctt caaattcttc ttttgtccag taaggaacga ttgattttcc ttttggaatt
    18001 gcttttgttc ttttggaaat attctcattc agaaattgca gggtgaccgc atagtctaat
    18061 gatttacgaa acataccgta cataagtgaa ctgtatgctt gtgagtatcc gctatcgttc
    18121 aataaccaaa ttcgataatt ttcacaatct cgcacattga tttcacgaag cttcttatca
    18181 ccgaaaaatt cagtaatctg aatcaatcca cactcacgag aactccaagt acttttttgt
    18241 acgctagctt tgtagtgtgg aacgtaagac tgctccataa attctttgta agttaaatca
    18301 taattcgcaa atccatttac aaccatgtat tcatttttta tacgcatgac ttctttgtgt
    18361 gcttcacgag ctgatgagaa tcgttttcct agagaatctt ttgttccttt cttctggatc
    18421 ctctttccag tcacttgatc tgttcctaac tcaactagat aagaaaagcg accattttta
    18481 tcttgataca cacaagggta ttttgttttc atagttattc ctcctgtgaa agaggaacac
    18541 ctataatttc ttcaattact tgtcggggta ctaccccaac tcttttatta ttataatatg
    18601 gatatccctg atttaccatt cttgtttttg cttgtcggat tattgtggta gcttgatgtg
    18661 ttttaaatcc tagagcctct aaatcttttc gacaaattgt atttttcatt gtataaactc
    18721 actttcaaag aaaacactat cattgggtaa tagtgttatt tctttatttt tgtataatga
    18781 acggtacttt cttgaaaatc catcacttgt tcaggacttc ccaactcatt taaagctaaa
    18841 gcattgtaaa aaaatggacg tctaactata tattcgcttg tatatagtat atcttgattc
    18901 gtggaatcat agggtttctt taccatcttt ttggtgacag ttggtagttt gagattccgg
    18961 gacttgaaaa aagctttttt cttatgctct ttcaattcca gctctttatc gaaatatttg
    19021 ctgatatagc gcccgcgatt ttcgatactg tctacatcga tcttgttgat cttaacgaaa
    19081 ccctgacccc aaatcgtagt taaccggtta aatgagataa aggggaaatc aaacaagata
    19141 atatggtagt ggattgcacc gcgttgctgc ttttcccacg tggcgagata ctttaaattt
    19201 gcttttttag ttttatagag gtcataattc agtcgtttga taaatttttt aaattcatta
    19261 tttgcatact caatgtcttg aatgttttct ttgaaagtga gagttaaaaa tttcgtttga
    19321 ttgtcaaaat tcgtgtctac aaggcgggca atctcaaatc gcatactttg ataatgtttc
    19381 tgtttgcgtt ttaaactgtc atattgtcct tgagcggata attcatcata gttttttctt
    19441 ttttcaagag taatatcatt ttcataaatc caagctaatt tttgttttcg ttccagattt
    19501 acttcgtttt cgatcgtttt acgtttggga gtaatgattg cttcataatc ataaatttca
    19561 atatacgtgg gagtttcaat gattttctga ttataagctt gcaaaaatca tcactccttg
    19621 ttgcgtattc gattgtcaaa gaaccagtca ttttctcctg atgttgatct ataaatcaag
    19681 ttaaggggga aggcctgcgt agcagccctt ctagcctatg gggtgttggg cggtcttaac
    19741 ggacagaaac ggctctgtgg gcgctgcccc gccgtttcgt cctaaccacc caacactcca
    19801 tagtatagtt agttttgtgt agtggtacct tgttttctgg gaatcaaagt tgccgtatca
    19861 gcggttgccg ataacccata actgacaaat tgtccattcg agcgtcccca aggtacaatc
    19921 accaaattgg taaatgtaat tatggctaaa ccttgttggg taagttcttc cggcgtgatc
    19981 acaggatgtt cagatttgac tttgacactg attttttcgt agcgaagttt tggtaggata
    20041 cattcgtagc tccaacctag aatttcgccc tccttttgcc agcgagtgac attgacgact
    20101 tggtattctt tttcgagtgt ttcttttggt aaactgagat cggtaatttt tattggcatc
    20161 acaattctcc ttttttatac gaaagtttat acgatttgat taaaaaatat agatggccat
    20221 ctacgacttg tattatatac gagaacttat acgtagtcaa gagattttct aaaaaaatat
    20281 atacgatttt ttatacgagt gttataatga aaaaaatgat aaggaggaat ttgaatatgt
    20341 ttggtttagt acaacctaat aaagtgaagg tgggccaacg aatcaaagaa ataaaagacg
    20401 gaatgtctct ctcttttaca gaattaggga atcgattagg tttaaaaaag ccgacgatca
    20461 gttcctacgt tcaaggctat gcgttggctc ctaagagtgt gatcaaccaa ttatctagta
    20521 tcagcggaaa acctgtcggc tggttttatt ttgggcatgt ggaagaatat atccgagagt
    20581 atttgtttct cattggtcag aaaaaaatag tggacgatta tcctgaaatc attgagaaaa
    20641 taaagcaaga attttatacg ggcgagttca aaaatcctgc ttgggagaat gagctaggct
    20701 atcctttgga ggaatttatc caagaatgtt ttgtagaata ttcgagtgaa atcatgaagg
    20761 actatgtcgt acagattacg aaagaggtac ttgaagaatc caaaacgcta aacaaactat
    20821 catcaaaagc aaaagaagaa gctgtcacga ttctttcatc tgggatcatt gactatatcg
    20881 agatgagtgg tgagatcaag tatggagaga aagatcgcat tgcgaaaatg gtgaaagaat
    20941 caataaaaac ctttgatgtg aaacaagacc tgacatttaa tgattcctat ttgattggaa
    21001 agttgatcaa tattcttggt acagatgaag atacggagct gtttctttct tctttatcaa
    21061 tgatatttac tgataagcat ttttccactc gatttggtgg ggaagaatta gtcgaaatat
    21121 ttcaatcgat gcgaccagcg ttaatcaaac tctatcggga gaaaacacca gatgaatttt
    21181 atgattggtt tgagaagtaa aataagaaaa gtggcttaga ttacaagttg gtgaaaaaat
    21241 ttttttcagc ataacatcag taatctataa aggggttgtt gattatactg tagaagttaa
    21301 gtactcaaga gattttttgt acgtaaacaa agcaaaaaag ggtgcagttt cactagaaaa
    21361 tgtactcttt tttgtgattt ttcttagtta tagagttttt ttgaaacttt aaaaatgaga
    21421 aaacatattg attttaaaga gtgtagacat agcttctttt gctaaataga ttaaacttac
    21481 tggtgagtgg tcttgaacat acttttaaat cacattttac tgagttttat tggattttat
    21541 aattcattgg aaacctcgta cttaatctgt ttttatctat cgactagaat ctgtatatat
    21601 tccagctatc aatgaggccg cagaaaaggg tgcttaaaaa taatctgttg tttaaaatat
    21661 tattatcaga atacttagat acaaaattct ggcacaaagt aaaaaaaata ttgacttaaa
    21721 aaaatggata tggtaatcta taaacgaaaa tagtggtaac gtttccaaaa tgcatcttcg
    21781 ttaaattttt atttttttaa acgaaaatgg aaacgtttct aaaatgtatt gggaggagat
    21841 tgaattggaa aagacattgg gaccaggtgg tgagataagg gcggtacaac gggataaaaa
    21901 aaatatttcg aagtgggaaa aattaaagaa acagttgcgg cgtgataagt ggatgtactt
    21961 gttgctctta ccaggtatca tcatgacgtt tatctttaag tatgtgccaa tgtacggtgc
    22021 tgtgattgca tttaaagact ataatcctat gctaggaatt ttggaaagtc cttgggtagg
    22081 actagatcac tttagggaat ttatcagttc accaaatttt acgcttttgc tgagcaacac
    22141 attaaaaatt agtatttatg gtttattgtt aggttttttt ccaccaatta ttttagcact
    22201 gagcttgaat caaattatca ataaaaaaat aaaacagaaa atccagttga ttctatatgc
    22261 gccgaatttt atctcagtag ttattattgt ggggatgctg tttttgttcc tatccgcaaa
    22321 tggacctatt aatgggttag tagaaaaggt tacggggcaa ccaattatgt tcatgtctaa
    22381 tccagcttat ttcagaacga tttatatttt atcgggaatt tggcaaggaa tcggctggtc
    22441 atccattctt tacaccgcaa cgctggcggg agtcagtacc gatttatatg aggcagcaaa
    22501 tatcgatggg gcaaatattt tacaaaagat ttggcatatc gatttgcctg cattgaggcc
    22561 tgtgatggtg atcagcttca tcttggccgc cggcggtatt atgaatgtag gatatgaaaa
    22621 agcctacttg atgcaaactt caatgaacat ccctgcctca gaaatcattt ccacttatgt
    22681 atacaaagtc ggcttgcaat caggtgatta tgcctactct gctgctgttg gtctgttcaa
    22741 tatgattatt aatgtcgttt tattactgat tgtcaacaat ataaccaagc ggctaaacga
    22801 tggtcaaggc ttgtaatgaa aggagaaaat tatgaatagt gctttatcaa aaatcgaccg
    22861 tcaaatctta tggttgaatc gagtgattgt aactttttta gtcgtcatca ccattgttcc
    22921 gctgatttat gtactggtag cttcttttat ggacccagct actttgatca ataaaggtat
    22981 aagcttcaat ccgaaagatt ggacaataca gggatatcaa cgggtatttg cagatgattc
    23041 cattttacgc ggttttgtaa attcgttgtt ttactctttt agcttcagct tattgactgt
    23101 atttattacg atgatcactg cgtatccttt gtctcgacct aacttggcgg ggaaaaagat
    23161 tattatgact ttttttgtca ttacaatgtt cgtgggtggt ggcttgatac ctacctattt
    23221 attggtaaag aacttgggca tgttagatag tgtatgggcg attatcgtac caggtgctat
    23281 aaatgtgtgg aatattatct tagcccgtac ctattttagt gctttgccaa aagaactttc
    23341 tgaagctgct actatcgatg gcgccaatga cttgcaaata ctgttcaaag tcatgcttcc
    23401 attagcgaaa ccaattatgt tcgtattgtt tttatatgcc tttgttgggc aatggaactc
    23461 atactttgat gccatgattt acatcaaaga tcccgactta cagccgttgc aactcgtatt
    23521 aagaaatatt ttgatccaaa accaaccttc acaagacatg gtgggagcca atactgccat
    23581 ggcagaaatg aaacaaattg ctgagttgat caaatacgca acgatcgtca tttctagtct
    23641 gccattaatc ataatgtatc ctttcttcca aaaatatttt gataaaggtg ttttagttgg
    23701 ttctgttaaa gggtaagtat tgttcactat ttgtatgaat aaaaattata aaggagattt
    23761 taagatgaaa gcaaaatgga tgctgccaat cgtttttact tccgtactaa ttttgggggc
    23821 ttgtggaggc tcaaaagaaa agagtaaatc aagccctgat tatgaactag aaaatgtctc
    23881 ttttccattg aaagaaaaag tatctttaaa aatgatgtct caaagtgccc ctttggctcc
    23941 caatgatcct aatgacaaat tgattttcca acgattagaa gaagagacca atctccatat
    24001 tgattggaca aactacagta gtgattttgc cgaaaaaaga aatttggata tcgctagtgg
    24061 tgatttacca gatgccatct tcaacgccgc tgcaggtgac tatgatttat taaactgggc
    24121 ggaaagtggc gttattgtac cgattgaaga tttgatcgaa gattatatgc caaatctgaa
    24181 gaaaattttt gatgaaaacc ccgaataccg acaaatgtct actgcacctg atggtcacat
    24241 ttactctatg ccttggattg aagaattagg cgaaggaaaa gagtcgattc atacagtaaa
    24301 tgggatggct tggatcaaca aggagtggtt ggataatttg ggtcttgaaa tgccgcaaac
    24361 cactgatcag ttgatgactg tgttggaggc tttcaagacg caagatccaa atggtaatgg
    24421 tgaagcagat gaaattcctt ttagctttat taacaatgga ggaaacgaag acttaaaatt
    24481 tttaatgggg gcattcggtc taggtgataa tgatgaccac ttagtcgttg ataacgatgg
    24541 tcaggtacaa tttactgcta acaaagaagg gtataaagaa gccatcaaat atttcaatga
    24601 aatgaataat aaaggtctta ttgataaaga ggctttcgaa caagattgga atacgtatgt
    24661 tgccaaaggg aaagaacata agtatggcgt gtactttact tgggataaag caaatatcac
    24721 gggtgcagat gatagttatg acgtcttgcc agtcttagcg ggaccagatg gaacgaaaca
    24781 tgttactcgt acgaatggca tgggtttctc aagagatcgt ttcgtaatta ctagtgcgaa
    24841 taaaaacttg gaacttacag ctaggtggat agataaaatg tatgaaccgt tacagtctgt
    24901 ccaaaataac tggggaacct acggtgatga agcacaggcg aatatttttg aattaaagga
    24961 tggtatgtta aaacatttgc ctttagaggg gacagcaccg ggggaactaa gacaaaaaac
    25021 cgaagctgga ggaccattag cagttcttga ttcttattac ggcacagtca ctactatgcc
    25081 agatgatgcg aagtggcgtt tagatttgat gcatgacacg tatttgccat atattacaga
    25141 agacaatatc tatccacgtg tctttttaga aaaagaagaa acagatcgat tagcaaaaat
    25201 cgaaacggat atgtgggatt acatctatcg caaaagagca gagtggatca ccggcggtaa
    25261 aatcgatgaa gaatgggaag aataccttgc tgaattggaa cgtgtaggtt tggaagaatg
    25321 gattcaaata aaacaaactg gctatgataa ttttttgaag ggagcacaat aagtgttagt
    25381 agaagaaacg gcgattttgg ataaagcaaa tcgatttatt gaaatgatgc gaggaaaagt
    25441 taaccctcag tatcgacaaa actatcattt ttccgcaccc atcgggtgga taaatgaccc
    25501 caatgggttt atatattaca aagggcatta ccatctcttc tatcaatact atccttacga
    25561 tagtcgatgg gggcccatgc attgggggca tgcgcgcagt agtgatttgg tccattggga
    25621 agatctacca gtagcgttag ccccagatca accttatgac agcggcgggt gtttctcagg
    25681 aagtgcaatt gaaagagacg gaaaactata tttattttat acaggctgtc aagaaattga
    25741 tggtattagc catcagcatc aatgtttggc ggtctctgaa gatggtattc attttagcaa
    25801 atacagtggt aatcctgtgg tagcagaaaa acagctaggt gaaaatgggc taatacaaga
    25861 atttcgtgat cctaaagtaa ttgttcacaa cgatggttac tatatggtag tagctactaa
    25921 atctacacag tctactggta gaatagtgat gtttcaatcg tcagatttgt atacttggca
    25981 cttttactct gtgctgctag aagggacttc agagcaagga ataatgtggg aatgtcctga
    26041 tttattccat ttagacggac gagatgtctt aatcatgtct ccgatacaaa tcaagaagaa
    26101 cggactggaa tatcataata tcagttctac catggcttgt attggtgagg taaactggga
    26161 aacaggtcag atgatagttg ataatttcca tgaaatggat tttggaatgg atttttatgc
    26221 accacaaaca acaagagatc ctaatggtgg acgggtgatg attgcttgga tgcagatgtg
    26281 ggatcgtaca ttgcctacac atgagttagg ccatctttgg gcaggctcta tgagtttgcc
    26341 ccgagagtta agagtgaaaa aaaatcgatt ggtacaaaaa cccatctcaa ctgtatatag
    26401 tcatttagtt tatcaacaag gcagtgaaaa tattgcagtg actgacacat cagtcgtatt
    26461 tcgaaacgcg ctgtctgatt cttcatattt acatctagtc gcagatttgg agaaagctga
    26521 agtttttaca attgaattac tgcggtctaa gacggattgt ctcaaactag cctatgacac
    26581 tgctacacag gtttttgaat tggatcgtca gaaactagga agagagctta caggggcgga
    26641 agaagaccct ctgaatgtac gaaaagttga agtacctttg attgataatc agctcgttct
    26701 tgagattttt agagatacct catcaattga agtctttatt aatgggttag aagttatgac
    26761 ggctactttc tatgagctgg aaaaagctcg agatatcttg tttcattcga tcggagaaag
    26821 tcgtattcaa gcttttgaaa cagcgagtgt gaaataaatg tttgattgct taggcgatga
    26881 atcaattatt taataggttt tgaaggataa tgtttttaga aaagaacaga tagctagatt
    26941 tcccatggat ttgctattag tattttgaaa aaattagtaa tttatgacaa aattcattat
    27001 actaaacttg tgcagaaatg tgagggatca actatgaaag caacaatgaa agatgtcgca
    27061 aaattagcag gtgttggagt aggaactgtt tcccgggtat tgaataacgg ttctgttaaa
    27121 gcttctacta gagaaaaagt tgaagcagca atgacagagt taaactatca accagatttt
    27181 tatgcccggg ggttaaagat gaagcatacg aacacggttg ctttgattct acccacggta
    27241 tggcatccat tttttggcga atttgcctac catgtagaaa cagcattaga aagacaaaac
    27301 tacaagcttt atttgtgtaa ctccagtggt aatagtgaaa aagaacaaga atatatccag
    27361 atggtcagcc aaaataaagt agacggcatc attggaatta cttattcaga tatcgatgaa
    27421 tacatttcga gtaatctgcc ttttgttagt attgaccgtt atttcactga agaggtgatt
    27481 tatgtgaccg ctgacaatgc ggccggtgga cgtttggctg caaatgaact ttatcgccga
    27541 ggatgtcaga atgtagcata tatcggtggg taccaagaaa caccgaatga aacgaagagt
    27601 cgtcgaaaat attttgaaat cgagtgccgt caactgggga tttcctataa agcgttagat
    27661 atgttagaac ctttagacca tctctcagaa cgtgtaacaa attttttgtt agagaatccc
    27721 gatatcgatg ggatttttac gatcaacgat ccaatggcat tagctgtcat tgatatgctt
    27781 gaaaaaatag ggaaaaacgt accagaggat gtccaagtta tcggatttga tggtcaaaag
    27841 atgacagaag gagatcctta tctagtctca acgattgccc aacctacaaa gcaaatggcg
    27901 gaagtggcag tggcaaaatt aattgctgtc attaatggag agacagtaga aaaacgtacc
    27961 gtattgccga ttgaatttgg cgaaggtgac acaacgaaac ctttagtgag aaacgatttt
    28021 gcttagtgca aatacgtttc tttttttgaa aatgagtgaa tgaaaggtgt agataaaatg
    28081 ttatatggtg gaatagaagc cggtggaaca aagtttgtct gtgggattgg taatgaacaa
    28141 ttacaagtga tagaacggaa aagttttcct accacaacac cagaagaaac gatggagcaa
    28201 gtgtttgatt tctttgataa gtacaaagga aatatcgcat caattggtgt aggagcattt
    28261 gggcctattg atattcaaat cgaatcaaga acttacggat atatcaccaa cacaccgaag
    28321 ttagcttggc aaaattatca ttttgtagga gctttggaaa acgccttgtc ggttcccatc
    28381 tattggacta ccgatgtaaa tgctgcttgt tatggagaat atatggcagg gtatggaaaa
    28441 ggtaaaaata gtgttctgta ttacaccgtt ggtacaggtg ttggtggtgg tgctgtagtg
    28501 gatgggaaaa tagtcgcagg ttttagtcat cctgaaatgg gacatatgct cttacaacgt
    28561 cataatgaag atcaatttga aggaatctgt ccttttcatc atgattgttt ggaagggtta
    28621 gcatcaggac ctgcgattga aaaacgcttt ggaagaaaag cccagactat ctcatttgat
    28681 catccttatt ggacgattga ggcagattat cttgctcagt gtgtgtataa cacaacgtta
    28741 ctatatgcac cggaagtaat aattttaggc gggggtgtaa tgaaacagcc gcaactactc
    28801 gagttgattc gccaatcgtt taaagaaaaa atgggttcct acgtggatac gcctactctc
    28861 gaagaatatc tactaactcc aaggcttgct gacaatgcgg gcttgattgg ctgcctagct
    28921 ttagcaataa agaaactcaa tcattagaat agtatttatt agaactattc ataacatacc
    28981 ggaagtttaa aagggtattc tgaaacttaa atagtctca
//
LOCUS       DASJTC010000028        27952 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.028, whole genome shotgun sequence.
ACCESSION   DASJTC010000028 DASJTC010000000
VERSION     DASJTC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.028"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            44..214
                     /locus_tag="U4B19_001091"
     CDS             44..214
                     /locus_tag="U4B19_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076847.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVRVKSKRP
                     TNHTKKWMAKNR"
     gene            434..1141
                     /locus_tag="U4B19_001092"
     CDS             434..1141
                     /locus_tag="U4B19_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2076848.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1200..1844)
                     /locus_tag="U4B19_001093"
     CDS             complement(1200..1844)
                     /locus_tag="U4B19_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEN2076849.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDEAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGIPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            1970..2257
                     /locus_tag="U4B19_001094"
     CDS             1970..2257
                     /locus_tag="U4B19_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076850.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2365..2685
                     /locus_tag="U4B19_001095"
     CDS             2365..2685
                     /locus_tag="U4B19_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2076851.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2766..4079
                     /locus_tag="U4B19_001096"
     CDS             2766..4079
                     /locus_tag="U4B19_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEN2076852.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLSNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKIL"
     gene            4076..4795
                     /locus_tag="U4B19_001097"
     CDS             4076..4795
                     /locus_tag="U4B19_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEN2076853.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVNSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQIAQPKIIRTISLAR"
     gene            4923..5474
                     /gene="raiA"
                     /locus_tag="U4B19_001098"
     CDS             4923..5474
                     /gene="raiA"
                     /locus_tag="U4B19_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEN2076854.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTANAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5696..8230
                     /gene="secA"
                     /locus_tag="U4B19_001099"
     CDS             5696..8230
                     /gene="secA"
                     /locus_tag="U4B19_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEN2076855.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8356..9457
                     /gene="prfB"
                     /locus_tag="U4B19_001100"
     CDS             join(8356..8427,8429..9457)
                     /gene="prfB"
                     /locus_tag="U4B19_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEN2076856.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9562..10248
                     /gene="ftsE"
                     /locus_tag="U4B19_001101"
     CDS             9562..10248
                     /gene="ftsE"
                     /locus_tag="U4B19_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEN2076857.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10232..11125
                     /locus_tag="U4B19_001102"
     CDS             10232..11125
                     /locus_tag="U4B19_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2076858.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11277..12725
                     /gene="cls"
                     /locus_tag="U4B19_001103"
     CDS             11277..12725
                     /gene="cls"
                     /locus_tag="U4B19_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN2076859.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13062..13919
                     /locus_tag="U4B19_001104"
     CDS             13062..13919
                     /locus_tag="U4B19_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEN2076860.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14034..14954
                     /gene="pstC"
                     /locus_tag="U4B19_001105"
     CDS             14034..14954
                     /gene="pstC"
                     /locus_tag="U4B19_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEN2076861.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTITTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            14954..15838
                     /gene="pstA"
                     /locus_tag="U4B19_001106"
     CDS             14954..15838
                     /gene="pstA"
                     /locus_tag="U4B19_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEN2076862.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15853..16659
                     /gene="pstB"
                     /locus_tag="U4B19_001107"
     CDS             15853..16659
                     /gene="pstB"
                     /locus_tag="U4B19_001107"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN2076863.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16675..17433
                     /locus_tag="U4B19_001108"
     CDS             16675..17433
                     /locus_tag="U4B19_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEN2076864.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17446..18123
                     /gene="phoU"
                     /locus_tag="U4B19_001109"
     CDS             17446..18123
                     /gene="phoU"
                     /locus_tag="U4B19_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEN2076865.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18361..19392
                     /locus_tag="U4B19_001110"
     CDS             18361..19392
                     /locus_tag="U4B19_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN2076866.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19389..20156
                     /locus_tag="U4B19_001111"
     CDS             19389..20156
                     /locus_tag="U4B19_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEN2076867.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            20256..21824
                     /gene="liaX"
                     /locus_tag="U4B19_001112"
     CDS             20256..21824
                     /gene="liaX"
                     /locus_tag="U4B19_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEN2076868.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22121..22441
                     /locus_tag="U4B19_001113"
     CDS             22121..22441
                     /locus_tag="U4B19_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEN2076869.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPFARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            22441..22791
                     /locus_tag="U4B19_001114"
     CDS             22441..22791
                     /locus_tag="U4B19_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEN2076870.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            22958..23896
                     /locus_tag="U4B19_001115"
     CDS             22958..23896
                     /locus_tag="U4B19_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEN2076871.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANKNGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23911..24741
                     /locus_tag="U4B19_001116"
     CDS             23911..24741
                     /locus_tag="U4B19_001116"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEN2076872.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24785..25810
                     /locus_tag="U4B19_001117"
     CDS             24785..25810
                     /locus_tag="U4B19_001117"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN2076873.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25825..26763
                     /gene="galU"
                     /locus_tag="U4B19_001118"
     CDS             25825..26763
                     /gene="galU"
                     /locus_tag="U4B19_001118"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN2076874.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            26888..27328
                     /locus_tag="U4B19_001119"
     CDS             26888..27328
                     /locus_tag="U4B19_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEN2076875.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27331..27933
                     /locus_tag="U4B19_001120"
     CDS             27331..27933
                     /locus_tag="U4B19_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN2076876.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
BASE COUNT         9263 a         4800 c         6047 g         7842 t
ORIGIN      
        1 ttaagataga gttaagttaa ctacaagaat tggagggatc cacatgaaat ggaaagaaaa
       61 agtagacgaa gcagctgaaa agttatatga tttggtcaaa tcagaaaaat acaatattga
      121 agtgaatatt ccgaaaaaag gcggaaaagc ggtccgggta aaatcaaaac gcccaacgaa
      181 tcatacaaaa aaatggatgg ctaaaaaccg ctaataataa caaaagatcc ataagtcggc
      241 attttcatcg attttagggt cttttgttat tttctaggtt ggacatatct aaaaaattgt
      301 ttctgacaat aggagtagga agaacgatgt gtgtctaaat cctctgttga tggatagaaa
      361 cctttatctt tttcagttgt gaatcaggta tataccagtt ataatagaag aaacgaaagg
      421 acgggtgtac gttatgtcga atcaattacc tgtatatata cagattcatg accaattaaa
      481 aaatgaaatc gaacaaggta tatggaaagt cggagaccgt cttccttcag aaagagaatt
      541 agcagtgaaa ttcaatgtca gccgtatgac gttgagacaa gcaattcaaa acttggcaga
      601 tgaaggaatc ttagaaagaa agatcggttc gggtacatat gttgcacgag aaaaagtaca
      661 agaaaaaatg tctggtgcaa caagttttac agaaattatg gaatcacaaa atcgagtacc
      721 gtctagtcgg acaatctctt atttcttgac ttcgccaagc tcaagtgaaa tggaaaaact
      781 aaacttgagt aaagatgaca cgatcttgcg aatggaacgt attcgtttcg cagatgatgt
      841 ccctatttgt tttgaagttg ccagtgtgcc acaaaagctg atcgatggct acagcaaagc
      901 agagattact gaatcgttgt atcgcacgct agaagaaaaa ggcgggaaaa agatcggtcc
      961 agcgaatcaa acgattactg ctgtgttggc ttctgagaaa attgctgact atttagaggt
     1021 aaaacgagga gatgcagttc ttcgtttgcg ccagatttct tattttgaag acggtacgcc
     1081 gtttgagtat gtgcgtacgc aatacgtagg aaatcgtttt gagttttatt tggaaaaata
     1141 aaaaacaggt gatccctgat tccgtattag aaataaaaga gaatcagaat cacctgtttt
     1201 tatatatgat cagttaccag ttgctcgtga taagaaacag gaccctcttc aaattggact
     1261 tgtccgttca gcagctcaat gatggattct ttaaagtggt cagcactttt ttcatctaca
     1321 gcacagatga cgctaacttg atcagtatat tggatttctt ttataaaaaa atgttggagt
     1381 tcaagataat tctgtaattt tcctagttgc ggatacgtga tcatcacaga gatctcctgt
     1441 tgcaaggtac cttctacgat tccaatgtca gacaatgctt gagagaccgc atgagtgtat
     1501 gctcgtatta atccgcctgt gccgagtttc gttcctccaa aatatcgagt gactactgca
     1561 gctacattga tcagttgatt ttttttgagg acttccaaca tcggtatgcc cgccgttcca
     1621 cttggttctc catcatcgct gcttcgctgg atttcatttt tttctccaat gatgaaggcg
     1681 ctgcagttat ggttcgcttt ccaatgttct ttttttattt cttgaatgaa actttttgct
     1741 tcgtcctctg attctattcg tctaagagaa cagataaagc gtgatttttt gatctcgatc
     1801 tcactttgtc cattttcttt tattgtacga tagcgctcga tcatcttgtc acctcttttt
     1861 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1921 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     1981 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2041 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2101 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2161 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2221 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2281 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcgtagt
     2341 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2401 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2461 gactctgata ttttcgctgt gtccgcttct gacgcagata acaatcttgc aaataaagac
     2521 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2581 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2641 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2701 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2761 ataatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattaaa gcaatagact
     2821 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgagcaact
     2881 ggattctttg tcatcgctgt ggaatgagaa cgtccgtcat tgatggacag cttgcagatt
     2941 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3001 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3061 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3121 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3181 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3241 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3301 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3361 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3421 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3481 acttaagtgc cacacctgat aaaaagttac taaaggagat caagcagacg tttgacatcc
     3541 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3601 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3661 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3721 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3781 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gacatactgt
     3841 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3901 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     3961 gtaaagggcc ttatacgaac agtcaagtat attttttcta tgaagaaaaa actgcagcta
     4021 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atactgtgag
     4081 gtgcagttgc tgcagtaaag atatcgtcca aaatttaaca ttcagcgaga ttattggtgt
     4141 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4201 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4261 gaaagtaaat tctatccccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4321 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4381 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4441 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4501 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4561 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4621 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4681 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4741 ttgtttgcag atcgcacagc cgaaaatcat ccgaactatt tctttggcga gatgaattag
     4801 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4861 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4921 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     4981 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5041 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5101 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5161 tggttgttga caaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5221 cccgtgaaac gggcatgaat actgcaaatg ctgctgcttt attcaatgaa gaggacgaaa
     5281 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5341 atagtgaaga agcagtcttg caaatgaaca tgctgggaca taacttcttt atttttgaag
     5401 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5461 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5521 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5581 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5641 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5701 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5761 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5821 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5881 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattaggtt tatatccata
     5941 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6001 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6061 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6121 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6181 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6241 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6301 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6361 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6421 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6481 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6541 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6601 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6661 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6721 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6781 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6841 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6901 tcgtccagat ttgctttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     6961 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7021 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7081 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7141 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7201 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7261 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca cagttctact tgtcattgga
     7321 agatgatttg atgaaacgat tcggttcaga acgaatcaaa tctttcttag atcggatgaa
     7381 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7441 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7501 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7561 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7621 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7681 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacagctca
     7741 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7801 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7861 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7921 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     7981 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8041 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8101 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8161 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8221 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8281 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8341 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8401 aaggtcacaa gcttcaggag gtctctttga cttagaccag ctggaagaag acattgcgga
     8461 agcagaagca aaaatggctg aacctggttt ttgggatgat agcgaagctg ctcaaaaagt
     8521 gatcagtgaa aacaatgcaa ataaagaaac ttatgatcaa ttccattctc ttgctgaaga
     8581 actggaagaa atcgaattga tggtggagat gttgcaggaa gaacctgatg cagccatgca
     8641 agaagaagca gaaacacgta tcaatgcgct agatgaaaaa atgaagacat atgaattatc
     8701 tatgttgtta gatggtcctt atgatcgaaa caacgccatc gttgaattgc atccaggggc
     8761 tggcggaacg gagtcccaag attggggcag tatgctttta cgtatgtata cacgctgggc
     8821 agagcagcat gggtatcaag tagagacact agattatcaa gcaggtgatg aagcaggaat
     8881 caagagtgtg accctactga tcaaaggaca caacgcattt ggttatctga aatcagaaaa
     8941 aggtgttcat cgccttgtac gtatctcgcc ttttgactca gcgaaacgac gtcatacttc
     9001 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9061 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9121 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9181 acaacgttca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9241 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9301 aatcggctgg ggttcacaga tccgttctta tgttttccat ccatattcta tggtaaaaga
     9361 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9421 atttattgac ggctatttaa aaatgcatct acaataagaa cccattgtta aattgaaata
     9481 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9541 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9601 ggtacgaccg ctatccgcaa tctctcaatt gtcatcgaac aaggagagtt tgtctatgtg
     9661 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9721 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9781 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9841 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgggat
     9901 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
     9961 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatagtg
    10021 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10081 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10141 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10201 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10261 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10321 cagtgcggta acgatcacat tgactttagt cggtgtattc atggcagtta tcatgaacgc
    10381 aactaaatta gctcaagata tagaaggaaa cgttgatgtc tctgtattcg tagatatcgg
    10441 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10501 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10561 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10621 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10681 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10741 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10801 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10861 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10921 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    10981 caatccatca ttattacgtt cccattattc attgattcat ccacaaaata tcgtttggaa
    11041 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11101 tatgagacgt ttcttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11161 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11221 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11281 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11341 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatgggtg ctcgttttga
    11401 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11461 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11521 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11581 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11641 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11701 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11761 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11821 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11881 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    11941 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12001 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12061 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12121 atacaaaaga agtaggctat tctcccaaat tttttccgtc cattcactct actggtgata
    12181 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12241 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12301 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12361 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12421 cagagctaat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12481 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12541 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12601 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12661 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12721 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12781 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12841 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    12901 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    12961 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13021 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13081 tcttaccaat aataggatta gctattcttt tatcaggctg ttcaaagtgg attgatcgag
    13141 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13201 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13261 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13321 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13381 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13441 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13501 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13561 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13621 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13681 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13741 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13801 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13861 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13921 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    13981 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14041 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14101 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14161 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14221 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14281 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14341 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14401 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14461 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gactgggttt ggtatcttag
    14521 ctggtacatt tgtgttgttc gttatgatct tgccgactat cacaacgatg actgttgatg
    14581 cattaaaggc agtgccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14641 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14701 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14761 cagcattgat gccaacaggt cttactactc ctgcttctac attgacgtcc atcttaacaa
    14821 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14881 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    14941 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15001 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15061 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggcgg
    15121 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15181 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15241 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15301 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15361 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15421 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15481 aacagtgata aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15541 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15601 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15661 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15721 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg attattgctg ttttactgtt
    15781 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15841 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15901 gagaaagaag tagcactata tacggatgat ctacatgtct ggtatggcga caatgaagca
    15961 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16021 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16081 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16141 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16201 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16261 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16321 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgtattgct
    16381 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16441 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16501 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16561 tatttaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16621 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16681 aaagagatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16741 gggatcgatc taagcttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16801 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16861 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16921 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    16981 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17041 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17101 cataaaagtg ctttatcttt atccggtggg cagcagcagc gtgtatgtat cgcacgtgtt
    17161 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17221 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17281 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17341 ggtcacttga tcgaatacaa tgatacgaag aagatattca tgaatccaga aaaacaagag
    17401 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17461 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17521 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17581 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17641 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17701 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17761 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17821 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17881 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    17941 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18001 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18061 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18121 taaactgaag ctgtgattat gccgtttgtt taggaaaaga ggttgtgaaa tcagcggttg
    18181 tgaaaaagct gttctgcatc aagtaataag aacagtcaaa caaaaaatag ctcctcatat
    18241 ttttcgtttg gctggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattcggga
    18301 aagggtttgt gacaaaacat gtcacaaacc cttttcatca tttcatagaa aggataaaga
    18361 atgcaaaaaa aaattagcat catcactata ttttttattt gtgtgcttac ttcatgggtt
    18421 attcagcatc aagccgcttt atatgaagta cctgtcatcg agatagaaaa agtacgaata
    18481 aaagaggatc atcaattgat tacaggaaaa ctgatgaaca cggatcaaaa aggagaatcg
    18541 atttatttat cggttcctta tcaaaaaaat caaatagaaa cagcaaatat aaaagcgaga
    18601 caaatgtatc tagtccaaat gcatggagat gaatggcagc tagtagcaaa aaaaaatgat
    18661 ggctggcttt tcttcttcac aagtatcttc attggcgcca tgttgcttat tggtggaaaa
    18721 cagggtctat ttgctttagg aagtctcatc atcaatatcg ttttactttt acttttgttg
    18781 ctcatcttta ctaaaacaga agggatttct ctattaaata tatctatcct gttcgtcgtt
    18841 atagggatga ttattagcat attggcttta gatggatgga atagaagcag tataatcaaa
    18901 atttcagctg cagttacgag tgttttttta gcatttttta tttgtctttt gacaatgaat
    18961 cattggaaag accaaggact gcgctatgag gaactgaatt atctgacacg accgtatcgt
    19021 tctgtttttc tttctagcgt actgattgga acagctggag gaacactcga taccgttatc
    19081 acagtcattg cgacattaga agaattgact aagcgacaac cagaaataac agcgaaacag
    19141 ttatggcttt caggaaggca agtgggtcga gatgtgattg gctcgatgac caatatcctt
    19201 ttgttcgtct atatcagtgg gtcagttcca tcgcttcttt tatatttagg aaatcaatgg
    19261 tcgttcaaag aaacgataga acagcattta tcattggaat ttttacgcgt cattgctggc
    19321 agcttaggaa tagtattatc gatccctatc tcggtcctct tttttctgac tgtaaggagg
    19381 ctgaaacgat gaatgtactt attatacttg ttgggatttt agcaatcagc gtgctactta
    19441 ttggcggtac gcaaggtatg tatattcttt taggcttatt catcaattta gggatctttt
    19501 tcctgttgtt attcggttat catcagaagt ggccgatact ggcattgtcg gtcataggat
    19561 ttttattgat tgctgtggtt atcttatttt ttatcaacgg ttataatcta aaaatgcgtg
    19621 cagcttttgt gagtatactg atctttttat tttgtttcct actattgctt cctatcactg
    19681 attttttagc aatccaaggt tttacatcgg tagaactaga agaattatct ggcttagata
    19741 agactttagc gattgatttt agattactta cgagatcgtt gcttctgatt agtcttagtg
    19801 gtgctgtttt agatgcgagt gtagcaattt ctagtgggac attcgaagta tatcaggcaa
    19861 atccgcaatt atctttttac caattgcgcc attctagttt cgctattgct aaaaaaatat
    19921 tggcgtcaac agtaatgaca cttctttttg cattcatggg aagttcttta gcgttgatcc
    19981 tctggtttat tgatttagac attccttttc agcaagtgat caatgaaaaa tcatttgttc
    20041 tagaatatac gatggctatt cttaccacat tgtccgcact acttgttctt cctttaacct
    20101 gcgtgactac agcatatctc tttacaaaaa agaaagaaca actcaagatg gagtaaaagt
    20161 tacaacttaa gtcctatgac atttttactg aatcagtgca taatatggat gtaaggaaaa
    20221 gcttgcaaac aattttgctt aaggaggcaa tttccatgaa agaaagagaa cgtattttag
    20281 atttagtgaa aaaaggaatc ctttcgactg aagaaggctt agatttatta gaaagcatgg
    20341 caactgaaaa agacgaaaag caaatcaaaa aagaagcaga caaggtaact gctagtcata
    20401 aagaaaaaga tcaggcaagt caattgatcg ataaacttga aaacggcgaa gaagaaattt
    20461 ctgagccagt tgatccaaag gaaagagaaa gacaagatca agaaaactta gaaaagatct
    20521 tagatgaact agcaactgaa gcgaataaaa cttcggcgcg tctagatgaa gtaaatgctc
    20581 aattcgctga caataaggca gcacgcaatg agaaacagga agcattgatg cagctgaata
    20641 caaaagaaga attaggcgag ttgacagaag aagaattagc acagcgtcag acgttagaag
    20701 cagaaatcaa agaattagaa gctagtggcg atacactgtt agaagaacaa ttgaaattag
    20761 aagcagaatt aaaagatatc cgtaagaatc aatggtctga gaaaaaagaa acgtttactg
    20821 ataagtttga attgccagat gattggaaag atcaagcaac agatacactg aatcaagtag
    20881 gcgaaaaaat gtcagaagct ggatcacagt taggcaaatt cttgaagaaa acattccaaa
    20941 ctgtttcaga aacagtgaat gacaacatgg aatggaaaga tgtcagctta cgtgtccctg
    21001 ggattgcgac aacgaaattt gagcatgaat tttactatga agctcctgca gcaagtatcc
    21061 ttgatatcaa ggcagcaaac ggaaacgtga cgttaaaaac gtgggacagc aacgatgtga
    21121 aagttgaagc taagatcaaa ctttacggaa aaatgggtgc tgaaccattt gaagcattct
    21181 ctgaacggag ccagattgag gttaatgaag atcatatctc cttccaaatt ccaaataaac
    21241 gtgtgcgtgc agaccttgtt ttctacttac ctaaacgagt atacgaccac gcagctatta
    21301 aacttctaaa tggcaacatc atgattgaaa cattggaagc taaagatatt tatacaaaat
    21361 ctacgaatgg taacatcatc gtggaccaat taactgcaac gatgctggaa gtagaaggtg
    21421 taaacggcaa tatcgatatt cgaaacggga acatcttaga ttctattatc gaaacagtaa
    21481 atggtacagt gacatttggt gcgacagcag aaaacctttc tgtgtctttg gtaaatggtg
    21541 atgtacgtct tacgattaaa gaagataatt tgaaaaaagt agaagcaagt tcagtgaacg
    21601 gaaatgtcaa agtcgcttta ccagatggca tcggtttgga aggacatgct aaaacaagtc
    21661 ttggaagtat caacagccgt ctgtccaatt acgaagtagt tcgtgagaaa aaagaacgga
    21721 caaaccaaat gttgcaattc cgtcgtgttt cagatggtga aatcgcgcaa gtgcaacttt
    21781 caacgacaac aggaagtatc tatttaaaag atacagataa ataaagaggt tgtgaaagag
    21841 atgttcagcc ttgagcaata agaagcgatt ttgtaaaata gcttccccta ttttacaaaa
    21901 ttgtgcctta ttgccgatag tctatctctt gaacaccgtt taccaagaga ttatgaacct
    21961 gccaattatc gccgagtcac aaggaataat ttcagaaaaa gctcgtcacg ttttatgaat
    22021 atggtaattt gtgaccgaga attttgcaga aagacaccga ttaagaagaa ggttgtcaca
    22081 acgattttct tcatttaaaa tagtagaaca gaggtgagac atgatgaaaa aaaagttgac
    22141 aaaatctaat aaaaatgttg ttttgacagg gactttagca ggaataggag aatacatcgg
    22201 gattgatccg actgttatcc gtgtgcttta tgtattcctg agcttagttg cttttggaag
    22261 tccaattatt ttatacattt tattagcttt gattatccca tttgcgcgtt ctgcacgaac
    22321 aaactatgga cataacaatg catattatga aaaaaacaat tatcgtgaac aaactcgtga
    22381 acaaacaaaa aaacgcaaag aagcggaaaa aatcgatgat gacgaatgga gtgactttta
    22441 atgtcgtact ttcaacgtat tttagttaat acgttaacat tcatatcatt ggctgttctt
    22501 tttcctgcga tggtccatgt gagcagtatt tggacggccg ttcttgccag cttcgtattg
    22561 tctgtgttga atatgtttgt caaaccgatt ttgacgatac tgtccctgcc ctttacatta
    22621 gtcacattgg gattgtttac gtttgttatc aatgcagcga ttcttaaaat gacctccttt
    22681 tttgtgggag cagaacattt tagtttttct tcattcggtg cagcgttgtt agtagctatc
    22741 ttgatgtcgc ttgtcaatgt aattgtcagc gaacataaca gtagaaaata aaagaaagct
    22801 gtgccgaact atcggcgcag tttttttatt tagaatcagg aatgcgtttt ctattacatt
    22861 ggtaattaaa taaaaacgtt ttttttaatt ggtagtactt ttggctagac aatgataaaa
    22921 tgatagagaa cactctaata gaaatgaggt agcatgaatg tcagaagttg taaaagttag
    22981 ccaattagtg aaaaaattat ctttggaaat cgtgacaggc gacgagaaaa gcttagaccg
    23041 tgtcatcgta actggggaca tctcacgccc aggactagag ttgactggtt attttaatta
    23101 ttattcccat aatcgtttac agctatttgg cagtaaagaa atcagttttg cggaacggat
    23161 gatcccagaa gagcgtttga tcatcatgag aagattatgt agtgaagata tgcccgcatt
    23221 tattatttca agaggattac cagctccaga agaaatgatc caggcagcaa ataaaaatgg
    23281 gatagctgta ctgcgttctc ccatctcaac ttctcgttta ttaggagaat tatccagtta
    23341 tttagatggt cgccttgccc ctcgaacaag tgtccacggt gttttagtgg atgtttacgg
    23401 attaggtgta ctgattcaag gtgacagtgg gataggaaaa agtgaaacag cgttggagtt
    23461 gatcaaacgc ggccatcgat tgatcgcaga cgatcgagta gatgtttatc agcaagatga
    23521 attaacggtg atcggagaac cgcctaaaat cctggaacat ctgattgaaa tccgaggaat
    23581 cgggatcatt gatgtaatga atctttttgg tgcgagtgcg gtacgtggct ctatgcaagt
    23641 ccagttggct gtttatctgg aagcatgggc aaaagataag aaatacgacc gtctgggctc
    23701 tgaagacacg accgttgaaa tcgcggaagt gggcattcca caaataaaaa tccctgtcaa
    23761 aacaggacga aacgttgcaa tcatcattga agtagccgcg atgaatttcc gtgcacgtac
    23821 aatgggcttt gacgcaacga aaacatttga agaaagatta agtcgcttga ttgaagaaaa
    23881 ctcaggcaac gactagcaag gagtgacaat atgttagcac aaatcaatcg agttgctttt
    23941 gagcttgtag ggattcctat ctactggtat gcattgatca tcgtatcagg tatcattatc
    24001 gctatgtggc ttagcagcag agaggctgtt cgggtaggat taaaagcaga tgatgtgaca
    24061 gattttatgt tgtggggatt gcctatctcc attattggcg ctcgcttata ttatgtttta
    24121 tttgatcttc cccagtatat agctgatcca atccaaatat tcaatatccg ttcaggaggc
    24181 ttagctattt atggcggact gattgcaggc gcgatcacgc tgtatttttt tactaaatat
    24241 cattttattt ctttgtggac ttttttagat atcgctgcac caagtgtatt attagcacag
    24301 gcaatcggtc gttggggaaa tttcatgaac cacgaagcat atggaccaga cacgacgcga
    24361 agcttcttag aaagtctgca tttaccgcaa ttcatcattg ataatatgta tatcgaaggg
    24421 atctatcgtc aaccaacatt tctttatgaa tcactttgga gtttgattgg atttattttg
    24481 cttttgtttt tacgaaagaa accacacctt ttgaaaaaag gcgaagtcct tttatgttat
    24541 ctaatgtggt atagtttcgg gcgtttcttt attgaaggat tacgtacaga cagtctttac
    24601 tttttaggtg tgatccgtat ttctcaatta ttatctgctg tgttatttgt cggtacattg
    24661 attctattta tttggcgcag aaaaaaagga aatttaccgg attatgaccg ctcattagga
    24721 aaaaatcaac aaattatctg atttttagag cgttaaaaat gaaaaaaatt tcaggagtgg
    24781 taaaatgaat tatagagtag cggttttagg accaggctct tggggtactg ctttggcaca
    24841 agtcctcgcc gaaaacggac atgacgttcg gatttgggga catcgtgctt cacaagtaga
    24901 agaaatcaac acccaacaca cgaatcaacg ttatttacca gatcattatc tacccgattc
    24961 catccaagca ttcaccaata tggaagatgc agtaaaagac gcagatgcgg tgctattcgt
    25021 cattccaacg aaagcaatac gttctgttgc aaaagaacta gtgcctaaat tgaatacaaa
    25081 gccagtgatc atccatgcaa gtaaaggatt agaacaggga acacataaaa ggatctctga
    25141 catcttgtta gaggaaatcc cagcagaaaa aagaaaagct gttgttgttc tttctggacc
    25201 aagccatgca gaagaagtag caaaacatga catcactacg atcacagctg caagtacaga
    25261 tgcaaaagca gcaaaatatg tacaagagct gttcatgaat aattattttc ggatctatac
    25321 caatcctgat gtgatcggtg tagagatggg ggcagctttg aaaaatatta ttgctattgg
    25381 tgctggcgct attcacggct tagggtttgg cgataatgca aaggcagcaa tcatgacgcg
    25441 cggattagct gagatcagcc gtttaggtgt agcaatgggt gccaacccgc tgacctttat
    25501 cggattaagc ggaataggcg atctagtcgt tacttgcaca agtgttcatt ctaggaactg
    25561 gcgcgcaggc aacttgttag gacaagggca gcccctagaa gaggtgctag ccaatatggg
    25621 aatggtcgtt gaaggtgtga atactaccca agcagcgatg gaactagccc aaatgatgga
    25681 aatcgaaatg cccatcactc aaacaatcta tgaagtgctg tatgaacaaa aagaaatcaa
    25741 aacagccgca aaagaaatca tgttacgtga tggaaaaaaa gaaaatgaat tctcattaac
    25801 aattttttga aggagcgaat gtatatgaaa gttaaaaaag cagttattcc agcagcagga
    25861 ttaggaacaa gatttttacc agcaacaaaa gctatggcaa aggaaatgtt gccaattgtt
    25921 gataaaccaa caatccagtt catcgttgaa gaagcgttga aatcaggtat cgaagacatc
    25981 ttgatcgtta caggaaaagc taaacgacca atcgaagatc actttgattc aaatattgaa
    26041 ttagaaacaa acctaagtga aaaaggaaag accgaattat tgaaattagt agaagaaacg
    26101 acagatgtca acttgcattt catccgtcaa tctcatccaa agggtttagg acatgccgtt
    26161 cttcaagcaa gagcctttat tggaaatgaa ccatttgtcg ttatgctagg cgatgatata
    26221 atggaagacg aagtaccatt gacacagcaa ttaatcgatg attacgaacg tactcatgct
    26281 tctacgatcg ccgttatgca agttcctcat gatgaaactt caaaatacgg aattatcaat
    26341 ccaggagaag tattggaaga tgggttatac aatgtcaaaa actttgttga aaaaccagat
    26401 ccaagcaaag caccaagtga tttagctatt attggacgtt acttattgac tccagaaatt
    26461 tttgatgtat tgtccgagca acaaccagga gccggaaatg aaatccaact aactgacgca
    26521 atcgatacat tgaataaaac acaacgcgta tttgcccgta aattcacagg taaacgttac
    26581 gatgtcggtg acaaatttgg ttttatgaaa acaagtattg aatatggtct aacacatcca
    26641 gaagtaggtg cacctttacg tgagtacatc ctgtctttag gtaaaacatt agaagcagaa
    26701 ggaagcaaga tcgatcataa tccaaaagga ataacagaaa tcaaagaaga aaagaaaaaa
    26761 taaccctttt ttctaacgag cttcagctgc taattgaata aaaagtagtt gaagctcgtt
    26821 tttatttgtt taaaaaacac gaaaatgcta tccttataga aaagagagac aagaagggag
    26881 tttgatcatg tcaggaggag aaattgcagg attgatcgca gcaatcgcct ttgcgatact
    26941 tgtcgttttt atcgttcgtg tgttgttgca agtttctaaa actttagaaa aagtagatca
    27001 gactgtagca gaagcaaata cgacgatcga aatcgtcaca aaagacgtag atcttttgtc
    27061 tagacaagta gaaggcttac tggtcaaaag taatgaactg ctcttggata tcaatggaaa
    27121 agtcgcaacg atcgatccat tgttcacagc agttgctgat ttaagtgaaa gtgtatcaga
    27181 acttaatcat tctagtaaaa acatcgcgac gaaagtcggc ggaatcggta aaacgacagc
    27241 taaagcgacg gttgccggaa aagtcggtgg gacagcgatg aaatttttta aaaacaaaaa
    27301 acatactgaa acgacaggag gaaaataaaa atggctaaaa aaggtggatt tttattaggt
    27361 gcagtagtcg gaggcacagc cgcagcaatc gccgcgttat tgttagcacc aaaatctggt
    27421 aaagaacttc gggatgaatt agctgcccaa gcggatgatt tgatggatat ggcttcagat
    27481 tacacagata tggcgaaaga aaaatcgact gagttaacag gtattgcaaa agaaaaagcg
    27541 caagatcttt ctcaacaagc aggtgatcta gcaggtagtg tcaaacaaaa agtaagtgaa
    27601 tcagcagatg aagcaggaag tgaatctgac gatatcttgt taaatcttaa agaacaatca
    27661 gctgacttat ctaatcgttt caaaaaaacg gcagaagata tgaaaaaacc tgcagatgaa
    27721 ttaggaagta ttgctaaaga tacttcagaa gatatcatga ttgatgtaaa agacgctgct
    27781 ggtaaagcag gagatactgt caatgaagga atgtcagaag caaaaccagt tgcagatgac
    27841 tcaaaagaaa tgatgaaaat ggcaaaagaa gatgcgaaag aagctaaaaa agaaataaaa
    27901 gatacaatga cagattcatc tacaaatcac taaaatcaca aatttataga at
//
LOCUS       DASJTC010000029        27555 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.029, whole genome shotgun sequence.
ACCESSION   DASJTC010000029 DASJTC010000000
VERSION     DASJTC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.029"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            158..1897
                     /gene="yfcC"
                     /locus_tag="U4B19_001121"
     CDS             158..1897
                     /gene="yfcC"
                     /locus_tag="U4B19_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEN2076877.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2054..3238
                     /gene="iadA"
                     /locus_tag="U4B19_001122"
     CDS             2054..3238
                     /gene="iadA"
                     /locus_tag="U4B19_001122"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEN2076878.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3349..3552
                     /locus_tag="U4B19_001123"
     CDS             3349..3552
                     /locus_tag="U4B19_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN2076879.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3653..4315)
                     /locus_tag="U4B19_001124"
     CDS             complement(3653..4315)
                     /locus_tag="U4B19_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2076880.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4328..5830)
                     /locus_tag="U4B19_001125"
     CDS             complement(4328..5830)
                     /locus_tag="U4B19_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2076881.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6378..8057
                     /locus_tag="U4B19_001126"
     CDS             6378..8057
                     /locus_tag="U4B19_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEN2076882.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8184..8663
                     /locus_tag="U4B19_001127"
     CDS             8184..8663
                     /locus_tag="U4B19_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEN2076883.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8882..9562
                     /locus_tag="U4B19_001128"
     CDS             8882..9562
                     /locus_tag="U4B19_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEN2076884.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9570..10901
                     /locus_tag="U4B19_001129"
     CDS             9570..10901
                     /locus_tag="U4B19_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2076885.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10916..11878
                     /locus_tag="U4B19_001130"
     CDS             10916..11878
                     /locus_tag="U4B19_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEN2076886.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11989..12327
                     /locus_tag="U4B19_001131"
     CDS             11989..12327
                     /locus_tag="U4B19_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEN2076887.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12406..13113
                     /locus_tag="U4B19_001132"
     CDS             12406..13113
                     /locus_tag="U4B19_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEN2076888.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13216..14697)
                     /locus_tag="U4B19_001133"
     CDS             complement(13216..14697)
                     /locus_tag="U4B19_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2076889.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15006..15542
                     /locus_tag="U4B19_001134"
     CDS             15006..15542
                     /locus_tag="U4B19_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2076890.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15620..16990
                     /locus_tag="U4B19_001135"
     CDS             15620..16990
                     /locus_tag="U4B19_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEN2076891.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17057..17392
                     /locus_tag="U4B19_001136"
     CDS             17057..17392
                     /locus_tag="U4B19_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076892.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17473..18330)
                     /locus_tag="U4B19_001137"
     CDS             complement(17473..18330)
                     /locus_tag="U4B19_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2076893.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18436..18951
                     /gene="tadA"
                     /locus_tag="U4B19_001138"
     CDS             18436..18951
                     /gene="tadA"
                     /locus_tag="U4B19_001138"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEN2076894.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            18974..19058
                     /gene="ffs"
                     /locus_tag="U4B19_001139"
     ncRNA           18974..19058
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U4B19_001139"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19268..20653
                     /locus_tag="U4B19_001140"
     CDS             19268..20653
                     /locus_tag="U4B19_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2076895.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSCLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20690..21367
                     /locus_tag="U4B19_001141"
     CDS             20690..21367
                     /locus_tag="U4B19_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEN2076896.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21375..22139
                     /locus_tag="U4B19_001142"
     CDS             21375..22139
                     /locus_tag="U4B19_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEN2076897.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22141..22800
                     /locus_tag="U4B19_001143"
     CDS             22141..22800
                     /locus_tag="U4B19_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEN2076898.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22830..23339)
                     /locus_tag="U4B19_001144"
     CDS             complement(22830..23339)
                     /locus_tag="U4B19_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEN2076899.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23451..24449)
                     /locus_tag="U4B19_001145"
     CDS             complement(23451..24449)
                     /locus_tag="U4B19_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2076900.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            24650..25234
                     /locus_tag="U4B19_001146"
     CDS             24650..25234
                     /locus_tag="U4B19_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2076901.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(25311..26504)
                     /locus_tag="U4B19_001147"
     CDS             complement(25311..26504)
                     /locus_tag="U4B19_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2076902.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            26568..26948
                     /locus_tag="U4B19_001148"
     CDS             26568..26948
                     /locus_tag="U4B19_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEN2076903.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            27022..27384
                     /locus_tag="U4B19_001149"
     CDS             27022..27384
                     /locus_tag="U4B19_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076904.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         8941 a         4663 c         5755 g         8196 t
ORIGIN      
        1 aaaaaaataa tcatacatag gagaaaaaaa acaaaataga aaaaagtgat tttctaataa
       61 attattcatg tggaaatgtc atttttactg tataattcat ttgattatcc attttttatt
      121 catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg
      181 aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg
      241 tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca
      301 agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga
      361 ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact
      421 gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt
      481 cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga
      541 tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa
      601 aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt
      661 tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct
      721 tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta
      781 tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc
      841 tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt
      901 gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta
      961 tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc
     1021 tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt
     1081 agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa
     1141 accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac
     1201 atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt
     1261 tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc
     1321 ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga
     1381 tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg
     1441 tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc
     1501 atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt
     1561 tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt
     1621 tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt
     1681 agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga
     1741 cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg
     1801 ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat
     1861 tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa
     1921 agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga
     1981 gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa
     2041 agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg
     2101 aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg
     2161 cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg
     2221 tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg
     2281 aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg
     2341 tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct
     2401 ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac
     2461 agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga
     2521 aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt
     2581 agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt
     2641 aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc
     2701 gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt
     2761 ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg
     2821 ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga
     2881 tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga
     2941 tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat
     3001 caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag
     3061 taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc
     3121 agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga
     3181 aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc
     3241 aacagctcta actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga
     3301 tgttaccctg aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta
     3361 aaaggacgtt ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc
     3421 aaagcaacag acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac
     3481 atcaaagaaa aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac
     3541 gaaaaaaaat aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat
     3601 atctgcgaga cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt
     3661 cgattttttg aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt
     3721 caatctcatg agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa
     3781 gtttgacgat atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga
     3841 ttagttgatg ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg
     3901 aaagtttggt cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat
     3961 ctaaagcaac ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga
     4021 cgttgtctag agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat
     4081 catttttacg ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct
     4141 ttccttcttt tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac
     4201 cacttgctcc taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat
     4261 aaagcgcatc ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat
     4321 tcctccttta gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta
     4381 aaaagacaga aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag
     4441 gtttgacatt gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg
     4501 ttccttgttg accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac
     4561 cgccaggagc ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc
     4621 ccacggcatt cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac
     4681 aaatagccac ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata
     4741 gcaagacgcc taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta
     4801 tgataatacc ggctacagcc actagtaaaa caacattttt agaaaaactt gccacgttgt
     4861 ttaaaggttc caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg
     4921 tgtcgatcag cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag
     4981 tgtaaatagc agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag
     5041 tgtaagaagt gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc
     5101 cgcttgtttc gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat
     5161 cttcagtact cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag
     5221 tagaatcaat gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg
     5281 tgatttttgc tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga
     5341 tttgaaagtc tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc
     5401 ctgcatcagt agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc
     5461 catcttttgc catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt
     5521 cagctatttt ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg
     5581 gacgttcttc agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt
     5641 ctcgattggc acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt
     5701 cttctgcagc gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca
     5761 cggcaaacaa taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca
     5821 taaaattcat attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta
     5881 aagctagctt aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa
     5941 aagtcactat agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac
     6001 gaagaagaaa tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac
     6061 gtgcatagtt gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa
     6121 tggggaacag ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat
     6181 tttgttaatg aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc
     6241 tggtaatttt cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat
     6301 tggaatggct catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat
     6361 aagaagaggt gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa
     6421 atggaaaaaa catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc
     6481 taaaatatcc tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt
     6541 tagaagaaaa cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga
     6601 tcggggcact tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga
     6661 cgatcgagtt agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt
     6721 tccatgtggt agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc
     6781 gtaccactca tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta
     6841 tcgtttatac gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt
     6901 atgcacgatt ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa
     6961 atgcagaaaa tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata
     7021 cgatcaaata ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag
     7081 tgcaacaagt attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag
     7141 atttcgaacg aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag
     7201 atctgcttgt tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag
     7261 aaactggccg aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc
     7321 gtaatgtcaa gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga
     7381 tggaaaccat cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt
     7441 tgatctctga gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga
     7501 tcaatttgat gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg
     7561 ctcatgcaga tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac
     7621 gcggagacgt gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag
     7681 aaaatgtaat gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc
     7741 gaaaaatctt gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa
     7801 agaaaatcat ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc
     7861 gtgatctgat gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa
     7921 gcaatgattt tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact
     7981 tatttgaaca aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac
     8041 gtcgaaataa aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg
     8101 ctcttttttt gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac
     8161 gatagaataa agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag
     8221 aacaaaagat cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag
     8281 aagaactaca aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag
     8341 gaaacaaaac tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa
     8401 aaacgagaca agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa
     8461 tgcatacagg atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg
     8521 attattgttt tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg
     8581 gtgaaatcgg gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta
     8641 tttacgctga tttaattagt taagggacga gaagagaagc taagcagagc agagacctca
     8701 aacttgagat tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt
     8761 tgtacatact gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga
     8821 tgtgcaagtg atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa
     8881 tatgaaaatt acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg
     8941 cgtgatgatt gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt
     9001 aaagacagat tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc
     9061 tattgcaaaa gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa
     9121 cagcagagga gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg
     9181 cgtggtaaaa ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca
     9241 gtatatgggc gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc
     9301 aggggatact gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt
     9361 cgctttcttg ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc
     9421 gcaaacaatt cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga
     9481 acaagatccc gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt
     9541 ctcggaaacg ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat
     9601 atattgaaag tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga
     9661 atgtcagtga aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt
     9721 caacaattca acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac
     9781 gtttgacatt tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag
     9841 gattagacaa ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac
     9901 gctatattac gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag
     9961 cattggaaga tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg
    10021 caaagctagc tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag
    10081 tcgcaaccat gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg
    10141 tcagtgatat ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt
    10201 ctgattatca gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc
    10261 atcatcgttt gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga
    10321 tcatcgatcg ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct
    10381 ctgttagcca tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact
    10441 tgacattgga aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc
    10501 gacaaccgca gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg
    10561 atactgaccg tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac
    10621 aaatggtcaa tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg
    10681 tcgcacaaaa aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc
    10741 tacattattt gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg
    10801 aaatcggcag acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt
    10861 ccaaagcaat cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac
    10921 agtacagcaa gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca
    10981 tttgcgccct gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga
    11041 aagtttccca gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt
    11101 agcagaaatg caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga
    11161 tacagctaat tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa
    11221 aatcgaccat catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc
    11281 aagtagctgt agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat
    11341 gaacgttaag gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt
    11401 gtatccctcc acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt
    11461 tgatgcaagt gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc
    11521 cggttatctt tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc
    11581 gcaagaaatt cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact
    11641 tcctggagtg attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta
    11701 ttaccgcgta cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca
    11761 tggaggaggc catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga
    11821 aatctataaa gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata
    11881 cttcgttcga aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc
    11941 tttttccgtt acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca
    12001 aattgcccag aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca
    12061 gaatgcggga acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg
    12121 gtattagatg caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac
    12181 ttgaaagtaa aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt
    12241 ttagtagaag gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg
    12301 aaatcggaat ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct
    12361 actactaatg ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga
    12421 atgtatggtt ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga
    12481 attgcgtcag cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac
    12541 aatgtgactg gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc
    12601 ggtgttcaac aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg
    12661 agtttgtctc aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat
    12721 gaatgcggac atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca
    12781 cttgttccag cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta
    12841 ttgcttagct ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta
    12901 cttttccaat tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt
    12961 ttgagtgaag gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt
    13021 gcagcagcac tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg
    13081 atcttattgt tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt
    13141 tgatctgagc taataccgag agttagctga ttgaatacca tttatttgga ataagaggat
    13201 gtgacaagaa ttttgtcaca tcctctttgt tttgttctca taaccttttt tcatatacag
    13261 ttcatgaacc gcttttatta acttgccaaa taacactgta ttgtgcgttg tatccagata
    13321 gaaagtctcc gtttcatctg aaattttctc atgctgatct gtaatgatca aatcagcatg
    13381 ttctgaatat gcggtaattt taacagcatc tttgcgaaat aactgctgca aatggctttg
    13441 aagatgataa tttcctgtaa actgtttggt tatctgcaaa tggatgagta actgctcttt
    13501 cgtttctgtt tgtagtaagt gttggatcaa ttgactaagg tattgactga tttggtttct
    13561 ttccccattt tttgaaaaaa ttgtatcgat tttttctctt aatttctttt cctgtataaa
    13621 agaactagaa tgattggacg tggcttctgg aaagcaaagt gtagaaaaac tactgctgga
    13681 ttcacccaac agttcgaaaa aaacaactgc caacacaaga aaataaaaat aaattggtat
    13741 tttattttct aaatggttga gaggaaactg ttgagttaag taagtcatca attgaccgac
    13801 ttgtcgacaa ataaggtttt tgcttgtgga aaataaaaaa ccaatctcct gtttttgtcg
    13861 aaaagagatt tgatttggaa aaaaacaata gaaaaaataa ttaaagtagg ctctctctgt
    13921 ttctgtctct tcacaagggc gactcactga aaaagaagta aagtattctg aataatcatg
    13981 tgtatgctgc aataagtcca tcaattcagt gatttcagaa gaagtttttg gaaaatatgc
    14041 ctgttgtttg tttcgtaata tgaataaagt taacagcaag tagatatttt tttcttgttt
    14101 tgaaattttt tctatttgct catctgtact gttcattttt agtgcattta attcaagtga
    14161 ggaaaatggc caatcgttac ctccaaatgt atctgaaaga aaaaagtaaa gatagagacg
    14221 gatagataac tcatttcctt caagagtaca gcaatttttt acagatagga tccgtaaatg
    14281 gaattttatt aaggaatgat tgagctgttt tgtcagtttt tcgagatagg agggcgatat
    14341 ataaagttgt tcaagtaaat atttttttgg tacagcagtt tgtggataat tgattagtaa
    14401 catcaaatag ttaaaacgat tggattcttt taggtagcat aattttaact tagaaaaaac
    14461 agctaacttt ttagtttgat cggtttcttt ggtttggatc tctcctttat gattagaata
    14521 aataaattca tcaatcaata aaaattgctt gagtgataca tttatttttc ggatcagttc
    14581 tttgattgtc cgttttggaa tatgtaagtc attggataat tcttgtaaag aacaattatt
    14641 tttttgaatg accatttgta gaacaagtaa ctcttttttt cttgcttcaa ttagcataaa
    14701 tacctccttt ttattctgtt tttgtatata gttagacaaa aactgtgagt ggaaagtcat
    14761 ttcagtctgt gttattctca cagagtgtcg ccttcttttt tatataacgt tatttaattt
    14821 ttttgttacc ttgtttattc tattgtttat ttcctagggt attttgtcaa gatattcttg
    14881 tttttaaggc ggaaaaattg atttttaatg gatataacta aatatgtttt gataattatc
    14941 aataatattt ttagaattat ttatgttatt tatttatggg tagatgaaaa gagacggagg
    15001 taagaatgat ggggagagta acaaattata gtaaagaata tttgctgttc aaaagtatgg
    15061 tatacgtgga agaatatgga atgaagtcaa ttactgcaag agagttagca gagttctgcg
    15121 gttgttctac atacccgatt tatacacatt tcaaatcaat cagcgggtta aaagagaaga
    15181 tattagaaga aatcactgtt tattttgaaa actacttagc tgaatgcagt tccgatgatg
    15241 ttttaagcat tgtctactta ttgaaagatt ttttttctat ccatgaaagt attcgtgaaa
    15301 tcattgcaaa atctgattta gacatggatt cgctatttaa gcggccattt ttctcatatg
    15361 tcaaaggtca cacgaagatc aaagaagaaa agtcgcttga attggtatgg cttaatgcat
    15421 tgggaagttt gcgctcagat gattcttctc aagattttct tgaaatgatg aacgctgttt
    15481 gggataaaaa cgaggtgacg gatgaatact tggaaaatat cttaaaggaa cagtcacctt
    15541 aggcagcaac atgaaaaagt agatcagcca cctttatttt tcgtaaaaaa gattgtaaaa
    15601 tgtctaacga aacattggaa tgaaggagga agcgacaacg ttaccacagc aatttatcaa
    15661 aaaatatcgt cttcttttag gcgaagaagc atccgatttt ttttctgcgc ttgaacaagg
    15721 aagcgtgaaa aaaggatttc ggtggaatcc actaaaaccg gcaggattgg acatggtcca
    15781 aacttaccat agcgaagaat tacaacccgc tccctacagc aatgagggat ttcttggcac
    15841 tgtcaatgga aagtctttcc tccatcaagc tgggtatgaa tatagccaag aaccaagtgc
    15901 aatgatcgtt ggaacagctg ctgcagcaaa acccggtgaa aaagttttgg atttgtgtgc
    15961 ggctcccggt ggtaaatcaa cccaattagc tgctcaaatg aaggggaaag ggttattagt
    16021 gacgaatgag atctttccga aacgtgcgaa gattttatca gaaaatatag aacgatgggg
    16081 agtttcgaac gcaatagtga caaatcatgc acccgcagaa cttgtccctc atttttcggg
    16141 cttttttgat cggatcgtgg ttgatgctcc ttgttcagga gaaggaatgt tccgaaaaga
    16201 tccaaatgca ataaaagagt ggacagaaga atctccgcta tactgtcaaa aacgtcagca
    16261 agagattttg tcttcagcaa taaaaatgct aaaaaataaa ggacaactga tttattcgac
    16321 ttgtacgttt gcaccagaag aaaatgaaga gatcattagc tggcttgtag aaaattatcc
    16381 agtaacgatt gaggagattc ctttgactca atccgttagt tcaggacgtt ctgaatgggg
    16441 aagcgtagct ggtttggaaa aaacaatacg tatatggcct cataaagatc agggtgaagg
    16501 acattttgtt gcaaagctga cttttcatgg acaaaatcag atgcataaag aaaagaagac
    16561 acgaaaaaaa agtaaagtgc aaatgacaaa agagcaggaa aaactctgga cggagttttc
    16621 aaatgatttt cattatgaag ctactggaag acttttagtg ttcaacgacc atctttggga
    16681 agttcctgag ttagcccctt ctctcgatgg tttaaaagtc gtacgtacag gtttgcattt
    16741 aggagatttc aaaaagaatc gatttgaacc tagctatgca ttagcgcttg ctacaaaaaa
    16801 gatagagaat ataccttgct tgccgatcac tcaaaaggaa tggcaatctt acacagcagg
    16861 agaaacattt caacgtgatg ggaatcaagg atgggttttg ctagtattag ataagatacc
    16921 tgtcggattt gggaaacaag taaaaggaac agtaaagaac tttttcccta aaggattgag
    16981 atttcactga gtggattagt tcgtttattt ttttgtctat gctatgatgg agatagataa
    17041 aaaggagctg atacttatga aaacatcaac aaaagtaaca atcggattaa gcgtagcagc
    17101 cgctgccagt gtagcgacag cagtcgttgt atcgggaaaa gtaatggaaa agatccatca
    17161 tatgactact cggacgaaag taaaaaaatt cgtctatgac aaatttgatg gaaatgaaaa
    17221 attactagaa atcgtagatg gtctttcaga tagtgacttg gattctttga tgggaatgtt
    17281 agcaaaaatc aaatcaggaa aaaagaaaat ctctgtttat ggtgattcaa tcaaagattc
    17341 aaccgaagat gtaaaagacc ggctaatgca ttttgttgaa aaaatgatgt aagagaaact
    17401 agcttttgaa cactattcat tcgaaataag gggttgtgac aaaattattg tcacaacccc
    17461 tttaatctat ctttaattat gttcatgaag caataaaaga tagcaaaaca gtaaatcgtt
    17521 ggcttttttt agattgaaac cagactgttc ttgaaatttt tcgattcgat attttagagt
    17581 attacgatga agaaacagtg tttttgcagc agagctgaca ttgcctaatt cattccataa
    17641 ttcttgaatg atcacttcca tttcattttg tttgatcagt tctatgtaac ttttgatcaa
    17701 cttactatct tttacagtat cttttgtata gtaatgtaaa gccacatctt gcatagaaaa
    17761 agtacggctg tgagtataaa gattgttttg ctctgttaaa aaaatttttc tttcttcagc
    17821 aaacaaaggg acgaggtcgg tgccaggtga atggaaagaa ccaacgaata cagacgttgt
    17881 cgtatcaaaa tcagcatcta aagatagaaa aataccgttg atttcagtag catcaagata
    17941 atcacggttt atttgttcga ctaagagcgc atctgtgtct gtataaaaaa agaaatcagc
    18001 agaatagcga aataattcca aaatagtatc ttgccattct ttttttaaga attctccttt
    18061 tgattcgacg tgaagctgga tcaaacgaaa gcttttttct tgaacacaag aagcatcttt
    18121 aaaaagatag tgataccatg ggtgtttttt ttgatcgttg attacaggga ataacgcttt
    18181 caaaaggctg gtttcttgtt gagataatga atcttgtttg atccagataa aagcatcatc
    18241 aattgcaaaa gaaaggatat ccgcttctga tgctggagag ttttttttct gtgcttgtgg
    18301 gtaaagagca agtaatttat ccattttcat cttgttcacc tcttgtattt attttaccat
    18361 agaaaaaaag ttagttacag cctttttatc atatgctaaa gtaaaacaag aaaaaacaaa
    18421 ggcagggaag ggtaattggt aaatacaaat cttgatgcag tagaaaaaga aaaatggatg
    18481 cgccttgcat tggcagaagc aaagaaagca gaaatgcttc atgaagttcc gattggagca
    18541 gtggttgtat tggatggaaa agtgattgga agaggatata accttagaga aaccacgcaa
    18601 gatgctacta cacatgcaga aatgcttgca atcaaggaag cctgtgaaaa agtgggaagc
    18661 tggcgcttgg aagatgctgc actttttgtt accttagagc cttgtcctat gtgcagcggc
    18721 gcgatgattt tgtctcgtgt agcagaagtt tatttcggcg cgtatgatcc aaaaggcgga
    18781 acagccggta ccttgatgaa tcttttggag gacgagcggt ttaaccacca agcatatgta
    18841 gaaggtggca ttttagaaga cgaatgtggg atgattttga ctgacttttt ccgaaagctc
    18901 cgccagcgaa aaaagaaatt aaaaaaagac tggtcaacga atgaaaaata aggtatacta
    18961 ttctttgccg aaaggctagg acaatggtgg gcttgtgaag cgtgtcaggt ctggaaggaa
    19021 gcagcactaa gcaggttccg ccatgtgtct gattataatg aaacgacaac agactttcgg
    19081 gtctgttttt tttattttta tagtactgta aaaatgaatg ttcctgattt tttagccaga
    19141 ctgatttatc gaatattgta cttatacaat ttgcttcaag gcaggaaaaa ctctgaaata
    19201 gagcttgttt caatggaaga aaaagctgcc tatggtatga taaagataaa gagaggcgag
    19261 agggtacatg gaaaaaatag tgattttaca tacgaatgat ctgcattctc atttagaaaa
    19321 ttggccaagg atcagaaggt ttttggatca acgtaaacga gaaaacgaaa agaaagaaaa
    19381 aacgacaacg attgcagtag atctaggtga ttttgtggat cgttggcatc cattgtcaga
    19441 agcaacgaat ggacaggaaa atatcttgct gatgaatgaa gttggttatg atgcagcgac
    19501 gataggaaac aatgaaggcg taggaaattc caaagaagag ttgaatcatt tatacgatca
    19561 tgctgagttt gatatcatcc tggataactt attcgataaa aatacgctcc agccaccggc
    19621 atggacgaaa ccatataaaa tcattacaac gaaacaccag acgaaaatcg gactaattgc
    19681 atttactgct ccatttccgc ttacttataa tccgaatggc tgggacatac ggaatcctta
    19741 tgatattttg ccagaattag tcgaagattt acgttcacaa gtggatgttt tagttttgat
    19801 gagtcatctc gggattcagg atgatttcca aattgcaaaa gaaataccag caattgatgt
    19861 tattttagga tcgcacaccc atcatttatt cagagaaggt cacgtaatca atgatgtcca
    19921 aatagcagcc gctggaaagt acgggcagta tgtcggtgaa gtccatttaa ccatcgatga
    19981 agagaaaaaa atccttaagc attcagccaa agccatccct accgaaacaa tgacagcttt
    20041 tacagaagat gaacaagaaa taaatagttg tctagaacgt gggcatgaat tattaaaaga
    20101 aaaagaagta gcagatttgc cattttctct gagtctagat atcttcaatg agcattcgtt
    20161 tatccaagta gcctttgagg cggtgaaaga acgaggaaag acggaagcag caatattaaa
    20221 cagtggtctg tttttaactg aaattccaaa agggcgggtc aatcaggatc agcttcacac
    20281 tgcgttgccg catccgatgc acttgttaaa tgtcacattg ggtggaaatg atctgatccg
    20341 cttagtttta gaaatagaaa aaaatcgtaa ctttttacgt aactatccta tgaagggaat
    20401 gggatttcgc ggtaagattt ttgggcagat ggtctataat ggaatcacat atgattctat
    20461 taaccatcaa gtcttttgga agaacaagcc aatcgatctg aataaaaatt atacttttac
    20521 taccgtagat catttgatgt ttgttccatt ttttccaacg attgagattg ctggaaaaaa
    20581 tgaatttttg ttccctgaat ttatccgaag tgtggtagga gattacttga aagctcacta
    20641 cccaatcaaa taaaaacaag gtataataaa aaagataggt ggtgaacgca tgtctgcaaa
    20701 acaaaatgaa cagcagaacg ttgagcataa aacagaaatt gctaagaaaa aatcatcagc
    20761 tgaacaggaa acaacagtca ccgaagaact gacagctgct ttggaagaaa tcgttgagga
    20821 gcctaaagca gaaaaagtca aaggcgagct agtgacactg ttagatgaca gtaatttttt
    20881 gataggtgaa agacattatc ggcttgtgtc cgattatcgt gaaggattta atgcagaaaa
    20941 gcttggagaa cgttacagtg atgtactagc ccgatatgat tatatcgtag gagattgggg
    21001 ttatgaacaa ctacgcctaa aaggattttt ccaagcggat aatcgacggg cgcatccaga
    21061 tcagcgaatc gattccttgg aagactatct ttacgaatat tgcaattttg gttgtgcgta
    21121 ttttgtcatc gagagaatcg gcggtaaaaa agaaaagaca actcagcgcc gaaaaaagaa
    21181 aaaaaaccat aatcgtgcgt atatcgatga gaaaaaagga ccagtcgcat ctaatcgcaa
    21241 aaaaccggtt attaaaaatc gaaaaacaga gtcagcaaaa acctctgaaa aagtaaaaaa
    21301 acaagaacaa cctaaacaag tagaaaaaac aaaaacagca tttacgatca gaaaaaggga
    21361 agagtagtgg atgaatggac tataaaggat atttaattga tttagacgga acgatttatc
    21421 gtgggacaca acccataccg gccggtaaac gatttgtgga agaattgcaa aaaagaaaat
    21481 tacctttctt gtttgttacc aataacacga ctaagtcacc agaaacagta gctaatcgtc
    21541 tagcagatga gtttgacatt catgtagctc cagagacagt ctacacagct actttggcaa
    21601 caatcgattt tatgaaagca gatggaaaag ggaaaaaagt ctatgttatc ggagaagcag
    21661 gattgattga cttgatttta gctgccggat ttacatggga agaagaggct cctgactatg
    21721 tagtggtagg cttagataat tatctgacct atgaaaaagt agttaaagca acacttgcta
    21781 ttcaaaaagg tgctactttc attggaacga acccagataa gaatatacct actgaacgcg
    21841 gccttcttcc tggagcaggg tcagtcatct cttttgtaga gacagcaacc caaacgccgc
    21901 cgatctacat cggaaaacca gaagccatca ttatggataa agccgttgaa gtattagggc
    21961 tgcaaaaaga agaagtaatc atggttggag ataactatga aacagatatc caagctggga
    22021 tacgcaataa tatagatact ttgctcgttt tatcaggttt tacaaaagaa gaagatgtac
    22081 ctgggttgcc tatcccagct acctatgtga aacaatcact ggatgaatgg agcttctaat
    22141 atgaaaaatt cgagatggca gtggatggaa tatgctggat tgttcagcct atttctgaca
    22201 ctgatcagtc tggcagttgg tgtgacgatc aactttcgac cactatacgt atttgatatc
    22261 ggacatttgc aaattttgga ttatacttct ttagatcaag agacgttact gaaaaatttt
    22321 gatcacctaa tgaattatct caataatccc ttcaaaacca tcctcagctt gccagatttc
    22381 ccagtttctg ctagcggagc ccatcacttt tatgaagtga aaattttgtt tttagtggat
    22441 tatgctgtgt ttttcatcac tttgatcccg agtattttgt ttatcaaata tttgcaaaag
    22501 aatgatcggc tttggcggtt gattcgtccg tttcaaatcg gcatgttact gccagttgtt
    22561 ttcggttttt tcatgatgat aggatttgat cgctttttta tcctttttca tgaaacgttt
    22621 ttcaacaacg atgactggtt attcgatccc gttactgatc caatcatcaa tgtacttcca
    22681 gaagaatttt tcatgcatag ctttatctta ttttttgtat tattagaatt attttttgct
    22741 gtttttcttt tcttaggaaa aaatagcttg aaacaaacaa aaaagaaaga actcgtctaa
    22801 atagacgttt tctttctttt tttgttgaat tattgagtaa ggcgacttct tttttctggt
    22861 tctttgtgtg ctaatcgact ggcagcagca gcggcgatcg ctccgacgat atcatctaag
    22921 aaggtatgga catgaacgcc atcgtggctg tttagttctt ttaaaatacc aggtttcacc
    22981 ttgtcgatat aaccataatt cgtaaagccg atcgtaccgt aaacattcac gattgacaag
    23041 gctaggatct catcgatacc gtaaaggcct tcgtcatctt cgataatatg ttgcaacgga
    23101 ctcatcaatt ttttcttttc tgctaatata tctaattgga ttccagtgat aatggcgtta
    23161 tgtacttcac gctttgttag cacgctgttg acactttcag tacaatccgc caaagtcaaa
    23221 tcagaaatat agtctttttg taaaaacatg acaagttgtg cgatatcttc tacagttacg
    23281 ccacgttcct tcaatagttc tcttgccttt tcttctaaag tctcagtttt gacgaccata
    23341 ttattgctcc ccctttattt tttctttctc tatttcatgt taacggaaca tgaaaaaaat
    23401 gcaagaaact gctttcgggt ttaacttgaa atgcaaagag atataaaaaa tcaactagga
    23461 cgaagatttt tatcgtataa gcttgtgctg tgaccaggct gagttcgttc ctttggatta
    23521 atatagtgta aagcgttgtt cactgcagta ggtgcttcac cgagcccagt agcaatcagt
    23581 ttcacttttc cttcataggt acagatgtca ccagctgcat aaacgcctgg aatagaagta
    23641 gacatatctg attttacagt aatggcattt ctggtactat caagtcccca agaagaaaga
    23701 tgttctaaat tagaagagaa tccataattg acgatcagag aatcaatcat caaatcaatc
    23761 gtctcgtcac ttttcacttt ttttaaccgg atatctgtca gttccccatt attgcctgat
    23821 aatccatcga tgagataggg agtaagcaag ttgacagatg aagatttcaa gcgtgataca
    23881 ctatgttcgt gcgcacgaaa ttccgggcga cgatgaatca agtaaacttc gcttgcaata
    23941 ggctctaaca tcaacgtcca atcgatagca gaatcaccac ctccagcaat agcgactttt
    24001 tttccagcat acttcatcaa atcattcacg aaataatcca atccgtggtt ttcaaatgat
    24061 tcagcattat ctaaattcaa ttttcttggc tggaatgaac catttcctaa agctaaaatg
    24121 actgctttgg aataatggat gcctttattt gtagtaatct cgataacttc gtcttcacga
    24181 gtaagcgaga gaacttcttc ttttaaatgg aatgtatggt taaaagtagt taattgtttt
    24241 tctaattgat cgattaattc acttgctttt attgctggat acccaggaat atcgtaaata
    24301 tatttttcgg gatacaaagt tgctaattga ccaccaagtt gaggtagact gtcaatgatt
    24361 tttgttttag cttggcgcat tcctgcgtaa aaagcggcaa acatccctac aggacctgca
    24421 ccgatgattg taatgtcata gatttccata aaaattcctc ccttttcaca ctatatcaga
    24481 atttaattga gaatgcttct caaaaatgtt tgaattcgaa atcgaaacgg taagttatag
    24541 aatgtatcta gtgatgaaaa ataaaagaaa acaaatctag tatttttacg agaggttagg
    24601 tattatgtta ttattattat gaagtatgca aatattagga ggaaacaata tggcattccc
    24661 acaactagat ttaaaaagtg aaaaaggacc aaaagctgtt atcaaaacaa accgtggtga
    24721 cattactgtt caattattcc ctgaactagc accgaagacg gttcaaaatt tcattgaact
    24781 ttccaaaaaa ggttattatg atggcgtgat ttttcaccgc gttattcctg atttcatgat
    24841 ccaaggcggc gatccaactg gaacaggaat gggtggcgaa agtatttatg gcgaaagttt
    24901 tgaagatgaa ttcagtcgtg aactgttcaa ccttcgcgga gctttatcta tggcaaattc
    24961 aggtccaaac acaaacggaa gccaattctt tattgtaaat aacacaaatg tgccagctaa
    25021 tatgcttgga caactagaag gtgctggatt cccatcagaa atcattgaag cgtataaagg
    25081 tggcggaact ccttggctag atttccgaca tacagtcttt ggacatgtac tagaagggat
    25141 ggacacagta gatgaaatcg caaatgtaca acgtggtcca caagaccgac ctgtacatga
    25201 tgtagtgatt gaaacaattg aaatttcaga atagtttggt atggtacgga gtaattatga
    25261 aaaaggacga aacctagtaa ggtctcgtcc ttttattttg aataaatgat ttaatcgatt
    25321 gtctctaaag catacttgat tcgattaagt ccttccagta aagtttcttc tggacaagca
    25381 atattcaagc gcatatgccc atgaccggaa ggaccaaacg tgatgccagg atttaatacg
    25441 acttttcctt tccgaacaag agtgttctcc aattcttggt cgtctgaaat ataggcggta
    25501 aaatctaacc acattaagta agttccttca gggttcatta ttctcacttt cggaagatac
    25561 tgttcaaaaa actgattaac tgtttgaaca tttttttcta gataaggaat cagttggctt
    25621 aaccattctc cacccgtctc ataagccgct tgggttccaa tcagtccaaa agtgttgatt
    25681 tcgtgctgtt ggttcatcac taaatgtttt tcaaaatcag ctttcatctc tggatttttg
    25741 ataaatacca ttgaattttt gattgcagct agattgaaag tcttggttgc ggaagtaaaa
    25801 gcaactagta gatcatctaa attaggatca atcgttagca tggaagtaaa tgtatgatcg
    25861 aataaagtta aatcttgatg gatctcgtca ctaaacagaa tcacttgatg ttttaaacac
    25921 aattcgctta actgtttcaa ttcttctttg ctccaaacac gccctccagg attgtgagga
    25981 ttgcataaaa tcattgcttt tacattgttt tcttcgattt ttttctccat gtcagacaag
    26041 tccatgatga aatgattatt tttttctagc aaagagcttc gaacgatgtg acgttgattt
    26101 gtttctataa tagaggaaaa tggaggataa actgggtcat gaatcaaaat agaatcgcct
    26161 ggttttgtaa aagtttgtac tgctgcagct aaactggtta acacaccact tgtaaaaacg
    26221 atattttctt tcgtcaactc cacagcatgg tgttggcgtt cccaatgaat gatagcctga
    26281 tatagtttat cagacagagt ggcataacca aagattccat gtttgatgta ttcactaaac
    26341 gctttagcta ccccaccggg agacaagaag tccatatctg ctacccacaa aggaagtaag
    26401 tcatccattt gatatgtctc agcaattgcg tcccatttta cactttctgt atcttttctt
    26461 gaaatctgtt tatcaaattc gatcatctta agacctccag tcattttcgt cgcttctttt
    26521 tttgagctat ttacagtata atgataaatg cgaagagagg gaagtagatg acttataaaa
    26581 ttggacaaat aattgaagga aaagtgacag ggatacagcc ttatggcgca ttcgtttctc
    26641 ttgataacga gacgcaagga cttattcatg tttcagaagt acaatccggt tatacgaaaa
    26701 atatccattc attgttaaaa gtcggacagc cagtaacagt tcagatcatc gacattgatg
    26761 aatattccaa aaaaatcagt ttatctttaa gaacgttaga aaaaacaaat ccagctgttc
    26821 catatcgcag aaaaaggtat ttcacgaata aaaatcgtaa gatcggattt accactattg
    26881 cggagcagtt acctatttgg atagaagaag cattatctga actagaacgg aaagaaaata
    26941 agaaataaaa agtgtatttt ttctgaaaat gtgctagaat ttttatgtaa gccatagaac
    27001 aagttgatcg aggtggaaat tttggcaagc aaaagagttg ctggtacaat tatgttgcat
    27061 ttggaggatg gtactaaaaa atttttggtc cattcaattg atgacaagtt ggaatttgcg
    27121 ttagcggaac tttctgaagg aaagactggt ttagcgaata tacttaattt cttaaaagaa
    27181 atagttcata tcgatgttag caagattagt ttgatggaat tgacaaatac acatattaaa
    27241 gaggaaaatg ttccgttgtt tgtctttgaa actgaacaaa agaatcaaag agaagaatta
    27301 ggcgaaggat atatttggga agaaccaggt caaatgcgct ctgttcttgg aacttacgaa
    27361 gtagagggag tgcctttctt ctaaaataca gattaagcga acaaagaagt ttgaattttt
    27421 ttcaaacttc tttttttatt gtttggtcca aaaaatacaa tgaaatgttc tgctaaagat
    27481 agttttttag tgcattttat tttcaaaata tgttgaaaac ggttgtataa cgaataataa
    27541 taattgatcg aaaaa
//
LOCUS       DASJTC010000030        27213 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.030, whole genome shotgun sequence.
ACCESSION   DASJTC010000030 DASJTC010000000
VERSION     DASJTC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.030"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="U4B19_001150"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="U4B19_001150"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN2076905.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="U4B19_001151"
     CDS             complement(937..1518)
                     /locus_tag="U4B19_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2076906.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="U4B19_001152"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="U4B19_001152"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEN2076907.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="U4B19_001153"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="U4B19_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEN2076908.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="U4B19_001154"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="U4B19_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEN2076909.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="U4B19_001155"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="U4B19_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEN2076910.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="U4B19_001156"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="U4B19_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN2076911.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="U4B19_001157"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="U4B19_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEN2076912.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="U4B19_001158"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="U4B19_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEN2076913.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="U4B19_001159"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="U4B19_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEN2076914.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="U4B19_001160"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="U4B19_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEN2076915.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="U4B19_001161"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="U4B19_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEN2076916.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="U4B19_001162"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="U4B19_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEN2076917.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="U4B19_001163"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="U4B19_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEN2076918.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="U4B19_001164"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="U4B19_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEN2076919.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="U4B19_001165"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="U4B19_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEN2076920.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="U4B19_001166"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="U4B19_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEN2076921.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="U4B19_001167"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="U4B19_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEN2076922.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="U4B19_001168"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="U4B19_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEN2076923.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="U4B19_001169"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="U4B19_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEN2076924.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="U4B19_001170"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="U4B19_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEN2076925.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="U4B19_001171"
     CDS             13972..14157
                     /locus_tag="U4B19_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEN2076926.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="U4B19_001172"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="U4B19_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEN2076927.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="U4B19_001173"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="U4B19_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEN2076928.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="U4B19_001174"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="U4B19_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEN2076929.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="U4B19_001175"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="U4B19_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEN2076930.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="U4B19_001176"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="U4B19_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEN2076931.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="U4B19_001177"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="U4B19_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEN2076932.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="U4B19_001178"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="U4B19_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEN2076933.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="U4B19_001179"
     CDS             18232..18879
                     /locus_tag="U4B19_001179"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEN2076934.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="U4B19_001180"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="U4B19_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEN2076935.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="U4B19_001181"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="U4B19_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEN2076936.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="U4B19_001182"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="U4B19_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEN2076937.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="U4B19_001183"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="U4B19_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEN2076938.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="U4B19_001184"
     CDS             20317..21255
                     /locus_tag="U4B19_001184"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEN2076939.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="U4B19_001185"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="U4B19_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEN2076940.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="U4B19_001186"
     CDS             21935..22774
                     /locus_tag="U4B19_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2076941.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="U4B19_001187"
     CDS             22750..23619
                     /locus_tag="U4B19_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2076942.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="U4B19_001188"
     CDS             23609..24406
                     /locus_tag="U4B19_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN2076943.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="U4B19_001189"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="U4B19_001189"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN2076944.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            26020..26257
                     /locus_tag="U4B19_001190"
                     /pseudo
     CDS             26020..26257
                     /locus_tag="U4B19_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(26401..27177)
                     /locus_tag="U4B19_001191"
     CDS             complement(26401..27177)
                     /locus_tag="U4B19_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2076945.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
BASE COUNT         8815 a         5005 c         5771 g         7622 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggt attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttgaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatgg tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggta gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttgaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcgagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttataa aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcatacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgattgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac cctcttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaag tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacatcctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tgtgacagaa gtggtcagct
    25381 tcaagaaata agaaggaatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag gagctacttc tggaacaccg tgaatcagag gttgtgaaaa agctgccctg
    25501 catcaagtaa taagaacaac caaacaaaaa tagctcgtca tatttttcgt ttggttgggc
    25561 ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaatcagcgt
    25621 tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt gccgtttgtt
    25681 gagctaatac cgaaggtcag ctgattgaac accgtttaag aaagaggtcg tgagcctgtc
    25741 atttaactct gagtcacaag taacaatttc aaaaaacagc ttctcatctt tgatgaatat
    25801 cgtgacttgt ggacgaggcg ttgataagtg aatcatcttt ttgttaccta ttttttctgt
    25861 aaccaaaaaa cgatggaacg ttgttttgat tgcccttaat gaggacagtc ggaacaggtt
    25921 tgtcattttt atatttaaat ggcacaaatt tttctaatta tttgtcattt agcgctgaaa
    25981 atttttgcta tttatctaaa aaagtgataa ctacttaaga tgaagcagaa tatttacgcc
    26041 atacggcata caaccgcgaa aaatataaaa aaaagaaaac gattgaacgc gtttttgcag
    26101 tcgcaaagga aaagcatgga atgcgctaca tcaagtatcg agacttggaa aacaatcagg
    26161 tgtatacgct gattgttttt gctgcaatga atctgaaaaa gttgtccact tggcagttgg
    26221 aacatatgtc ttcatatctt ttattataca aaaatagcaa aagcaccagt ctacactctt
    26281 agtaaagagg aaactgatcc ttttgtctac attcttaaag tacttaaact aagtacttta
    26341 ttttactctt taggattgat acagtagcat ctatcccttt attaataatc ttttattttt
    26401 ttaaataaag atgttgtctc catcttcagg cactaaaact ttttcggaaa ttcctttttc
    26461 tttattgaaa gactttaatt cttctcttga cagtccccaa tggtttacag cttccatatg
    26521 aacggcaata attgtggaat ctggtattgc tttatgcaat tcatataaat cgtctttccc
    26581 cataataaga gattctccaa ctaaaaaatg attatctcca gcatttgcta ctatgatttc
    26641 tggattatac tgatcaatag tttgtttaac agcttcatac caaactgtat ctcctgtaaa
    26701 gtatagtgtc ttctcagtcg aatgtttgaa aacaataccg caaacattac ctacataatt
    26761 caagatttct cctctgccat gtttgccttt tgttttaacc aattgtatag attcaaaaac
    26821 tgtgtcttct cgtagtacct ccacattttt aaaaccatat agtcgaattt cattcgcatc
    26881 attttcatct tgcacaaata attttatttc tttaggtagg acctctttcg ctatatcgtc
    26941 ccaatgatct agatgtaaat gtgttacaat aacagcatca ataccgttga taatatcttc
    27001 gactggcatt ggcaaatcaa ctagaggatt catttgatct tttcttggtg atggaaaagg
    27061 acctttcgaa ataaatggtg gaagtgttcc tttttccgct aacattggat caattaaaaa
    27121 ttttttacct ccatagtcta agatagctgt tgcattacga atgtgatgta tattcataaa
    27181 cgttgtctcc tttttgttga tatgaaatat ttt
//
LOCUS       DASJTC010000031        26959 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.031, whole genome shotgun sequence.
ACCESSION   DASJTC010000031 DASJTC010000000
VERSION     DASJTC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.031"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..480
                     /gene="manA"
                     /locus_tag="U4B19_001192"
     CDS             <1..480
                     /gene="manA"
                     /locus_tag="U4B19_001192"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEN2076946.1"
                     /translation="KKGDFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQ
                     GQTRELHIQQSIDVTTVPAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSF
                     KKQAPYTLMTVIEGAGDLVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            574..2277
                     /locus_tag="U4B19_001193"
     CDS             574..2277
                     /locus_tag="U4B19_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2076947.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            2279..4312
                     /locus_tag="U4B19_001194"
     CDS             2279..4312
                     /locus_tag="U4B19_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2076948.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            4323..4763
                     /locus_tag="U4B19_001195"
     CDS             4323..4763
                     /locus_tag="U4B19_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2076949.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(4963..6090)
                     /locus_tag="U4B19_001196"
     CDS             complement(4963..6090)
                     /locus_tag="U4B19_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2076950.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(6093..6770)
                     /locus_tag="U4B19_001197"
     CDS             complement(6093..6770)
                     /locus_tag="U4B19_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2076951.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            7034..8800
                     /locus_tag="U4B19_001198"
     CDS             7034..8800
                     /locus_tag="U4B19_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEN2076952.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            8815..9252
                     /locus_tag="U4B19_001199"
     CDS             8815..9252
                     /locus_tag="U4B19_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2076953.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            9265..10416
                     /locus_tag="U4B19_001200"
     CDS             9265..10416
                     /locus_tag="U4B19_001200"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEN2076954.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(10478..11176)
                     /locus_tag="U4B19_001201"
     CDS             complement(10478..11176)
                     /locus_tag="U4B19_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEN2076955.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(11169..12521)
                     /locus_tag="U4B19_001202"
     CDS             complement(11169..12521)
                     /locus_tag="U4B19_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEN2076956.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(12744..14144)
                     /locus_tag="U4B19_001203"
     CDS             complement(12744..14144)
                     /locus_tag="U4B19_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEN2076957.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            14305..15042
                     /locus_tag="U4B19_001204"
     CDS             14305..15042
                     /locus_tag="U4B19_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEN2076958.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            15167..15646
                     /locus_tag="U4B19_001205"
     CDS             15167..15646
                     /locus_tag="U4B19_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEN2076959.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            15643..16410
                     /locus_tag="U4B19_001206"
     CDS             15643..16410
                     /locus_tag="U4B19_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076960.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            16640..17209
                     /gene="lepB"
                     /locus_tag="U4B19_001207"
     CDS             16640..17209
                     /gene="lepB"
                     /locus_tag="U4B19_001207"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN2076961.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(17313..17915)
                     /locus_tag="U4B19_001208"
     CDS             complement(17313..17915)
                     /locus_tag="U4B19_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2076962.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            18021..18490
                     /locus_tag="U4B19_001209"
                     /pseudo
     CDS             18021..18490
                     /locus_tag="U4B19_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            18596..19180
                     /locus_tag="U4B19_001210"
     CDS             18596..19180
                     /locus_tag="U4B19_001210"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN2076963.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            19183..20256
                     /gene="prfA"
                     /locus_tag="U4B19_001211"
     CDS             19183..20256
                     /gene="prfA"
                     /locus_tag="U4B19_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEN2076964.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            20249..21088
                     /gene="prmC"
                     /locus_tag="U4B19_001212"
     CDS             20249..21088
                     /gene="prmC"
                     /locus_tag="U4B19_001212"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEN2076965.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            21081..21260
                     /locus_tag="U4B19_001213"
     CDS             21081..21260
                     /locus_tag="U4B19_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076966.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            21282..22307
                     /locus_tag="U4B19_001214"
     CDS             21282..22307
                     /locus_tag="U4B19_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEN2076967.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            22498..23742
                     /locus_tag="U4B19_001215"
     CDS             22498..23742
                     /locus_tag="U4B19_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEN2076968.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            23895..24842
                     /locus_tag="U4B19_001216"
     CDS             23895..24842
                     /locus_tag="U4B19_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2076969.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            24976..25605
                     /gene="upp"
                     /locus_tag="U4B19_001217"
     CDS             24976..25605
                     /gene="upp"
                     /locus_tag="U4B19_001217"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEN2076970.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(25744..25986)
                     /locus_tag="U4B19_001218"
     CDS             complement(25744..25986)
                     /locus_tag="U4B19_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076971.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            26107..26179
                     /locus_tag="U4B19_001219"
     tRNA            26107..26179
                     /locus_tag="U4B19_001219"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26140..26142,aa:Thr,seq:cgt)
     gene            complement(26298..26936)
                     /locus_tag="U4B19_001220"
     CDS             complement(26298..26936)
                     /locus_tag="U4B19_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2076972.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
BASE COUNT         9159 a         4807 c         5456 g         7537 t
ORIGIN      
        1 aaaaaaggtg atttctttta tgtaccaagc ggaacgatcc atgctattgg taaaggaatc
       61 atgattttag aaacacagca aagttctgac acgacttacc gcgtgtatga ctacgacaga
      121 aaagatgcgc aaggacaaac aagagagctg catatccaac aatcgatcga tgtaacaact
      181 gtccctgcga aaacaccaga attacaaatc aaagaagtcc gaaaagggaa ttcatcgatc
      241 gtcacttatt tagaaactga gtttttcaat gtatatgagt gggatattaa aggaatcaca
      301 tctttcaaaa aacaagcccc ttatacattg atgacggtaa tcgaaggagc aggagacctt
      361 gtcgttgacg gaaaaacata tccattagaa aaaggaacaa gctgtattat tccaaatggc
      421 gtttctgagt gggcggtcca aggtgaacta gcaatcattg cgtctgaacc aggcaaatag
      481 ttcccagata gctgaaagca gcagacggtt tttcgtttgc tgctttttct acataaaatc
      541 cgtccatcaa taaatagaag tctggtgaaa aagatggaat acaaatggaa agcaaacaac
      601 agcagtcgcg tgtctatgaa acaactgaga aaagctggac atcacttaaa tgaaatgatc
      661 atgccaaatc taagtctgtt tatcgcttgg ggaatcttgt cattagcaac aaaaggtgtg
      721 tctggcgatt tgcacaccac attagaagag gtatgccatt ggatgatcca gctgctttta
      781 cccatcttga tcagttattc aggcgggaaa tcggtagggg gacaaaaagg ggcagtcgcc
      841 ggtgcaattg cttctttagg gttgatcgct cgttccaatg ctccacaaat cgtaggagcg
      901 atgattatcg ggccgcttgc cggcatcgta tatgaacaat ttgtttctag attccatgtg
      961 aaattcaagg ctggatatga aatgcttgtc agcaacctac tagtaggact gactggtagc
     1021 gttatttgta taattggaat cgtttttatt gaaccagttt taaatagtgt tcacttgttg
     1081 ctcgcctccg ttgtgtcttg gctagtcaca atgaatctgt taccacttgt cagtctgatc
     1141 ttagaacctc taaaagtact atttttcaat aatgcaatca atcatggtgt attgacacct
     1201 ttaggaatcg aattctcaca aacagtcggg tactcttcct tatttttgat ggaggcaaat
     1261 cctggacctg gtttagggat cttactttct atcctttgtt ttgcagaaaa acaggaaaaa
     1321 gtaaatgcga gtggagcatt gatgatccat gcgattggcg ggattcacga aatctatttt
     1381 ccatttgtct tgctgcgacc gtatctattt ttagctgtaa tggctggtgg ggcaagcggc
     1441 acgttgatat ttcaatggtt agatacggga ttagctgcac ctgtatcgcc tgggtcacta
     1501 ctcgtgattt ttgccaatgc gccttatcca caactagctg gggtaagtat agggatagca
     1561 atcagtacat ttgtgacctt catttgtgca gcgatactat tgaaattacc aaataaagaa
     1621 aaagagaaca aaacggtcaa agaaagggga gaaatcttgg aaccatctat cattcattcc
     1681 gtcatttttg cttgcgactc aggtatggga tcaagtgcta tgggagccgc cctgctgaaa
     1741 aagaaattag cagcagaaaa aatcgatctt tctgtcggat acagctctat ctatgaattg
     1801 aataaccaag taaatcagct ggttatcgtg caacatgaac tagcgggtct tgcaagagaa
     1861 aaagtacctc aaagtcaaat ctatgtactg gatcactttt tggaaacaga tatcttgctg
     1921 tcacgaataa aagaagcgat ccaatttcca aagcgggaag aaaaaaaaga gatagcagat
     1981 atcacagaac aaaaaacgtc gcctgctaat attgtatttt tgtataaaaa caacagacgc
     2041 ggctcacaaa ctatgggcat gacgatttta agtcaattag caaaaaaagc gaaaaaacag
     2101 tttgttatct caaaagaatc aatagaaaaa atcaaaatcc aagaagaaac gatttatatc
     2161 gcacaaagag aactagctgt aactgaacag ctagaagaaa agatccctcg gctagttcta
     2221 gttgaacatt gggtagctac gaatgaatac gaaaaactcg tgaagggaga aatataagat
     2281 gtatttatcg cccagagaaa aacaattatt agcagaattt ctcagtagcc cgtcacctgt
     2341 atctatccaa aaaatgatga atttattgaa ggtcagtaaa cggactattt accgcgaatt
     2401 agataatctt gaagtctcat tgaaatcggt aaatgcttct ttgaaaaaag cagctagagg
     2461 ctcatttgtc attgaggcat cagaagaagt attagagcaa ttaaaagaaa atacagatgg
     2521 gaaaagtaat gacttatcca ctgctaaacg acaacacgcg attttagccg agttgttact
     2581 gacgacagag ccgttgagtc ttgctcattt catggaatca tatcgcatca gtaataccac
     2641 tttttatgca gatatcaaac agttggaaga aagtattcgc caactgcctt tggaaatcat
     2701 tcggaaccag ggatacgaga tcgaaggacc ggaaaaatac cgccggcttc tgacagcgaa
     2761 tgtattagaa ttagaaatca atgaatatga actttttcat agtatttctt tcgatgcatc
     2821 actgaattat ttttttcgat tcgttgatcc gcaacatcta tctcttgcac gcaaagtaat
     2881 cggggaagaa ttgatccaaa aaaagaccaa tctaagtgat agaaagcttg aacatttggt
     2941 tttgatgctg acattgacga tggagcgtgt gacgaaaaat catttactga caaatgagac
     3001 atataatagt ctggcaaaca aagaattact acagacagct aaacgattgt tttcacggat
     3061 cgcttctgag acaaaacaac tttatccagt aaatgagatc gtcttttttg caggattatt
     3121 aaatgacttc acgaactcat ttgatcagaa tttctttgaa gagacgttcg atacacagct
     3181 tgcttattta gtaaaacaac tgattcaaga agtaagtgaa gagacgaatg tccattttta
     3241 tgaagatgaa actttgtaca aaatgctgtt gactcattta tctggtgtgt tttcaagagc
     3301 cgttttacaa gaagagacac tgaccaatcc gattttagaa aaaatcatgg ttcaatacga
     3361 ggaagttgcg aatgcaatcc gtaaagcagt agagcggatt tttcaagaaa aaaaattatc
     3421 cgaagaagag atcgcttata tggtccttca ttttgcgaat tcattggaac gaagtccgaa
     3481 agaaagcaat atcagtattg caggattttc gccaagtgga ttagcttcta ctagcatgtt
     3541 ggaactacga ttgaaaaggt attttccctt catccaacaa attcatttct atcggattgc
     3601 tgatttaaaa aaaatcgatt tacaagaaga atatgatgtt gttatttcta catctatttt
     3661 accaggatac ggtggaaaat atattttagt ctccccgcta ctgctagaag atgaagtcaa
     3721 gcagctaaaa gaagcttttt cgcatattga gaagaaaaaa aggcaaaaaa ttacgtataa
     3781 cagcaataac gagtggatga tagaagatac ttatgaggaa acaatgcatt tcatggatca
     3841 aatcaatcaa ttactcggtc gtttttttat ccaagaactg gataatcctg agacgatttc
     3901 cgaaacgatt cgcagcttat ttccttattt tcctgaagga ctagtaacgg atgaagaggt
     3961 ggttcatcgc cggctgctta agcgatatga acaagcacct atcggtattc ctcatacaaa
     4021 tatggggtta ttccatacat ccagctcatt gatttctaaa ccgatctttt gtattttcaa
     4081 tttgaaacat ccgttgacca ttgaagggat ggatagacag ccagtcgatt tgtcccgtat
     4141 gttagttatg ctggcgcctt cgccaatcca agaaaaagac gggattcttt taggaaagat
     4201 cagtggagca atcatcatga atgacttgaa caccgagatc ttccgctcag gaaataaaga
     4261 aatcgtttat cagttgttag cgaaaatcct gattgaagaa ataaagaaat gaaggtgaaa
     4321 agatgataca gctaccagca gaaaaaatat tgctcgatca atcctatcaa aattggaaag
     4381 aagcgttgac cgagatcggc cagtggatga cccaacaagg gggaatcaaa ccggcatata
     4441 tccaatcaat gatcgaaaga caagaaaaag cgagtgtcta tatcgggaat ttcgtagcct
     4501 tgccacatgg gactgacaaa gatgaagaag tagtagaaga aggaatctat ttagtccaag
     4561 tgcctgatgg tgtggacttt ggaacaaaag aggaaccaaa aatcgcgaca ctcttatttg
     4621 tagtggcttt aaaaaaagaa catcagttag cggtgctcca agatttagcc tttttctgtt
     4681 cagacatcga ccaagtaatg gcgctcagtg atgcggaaac cattgaagaa gtccaaatga
     4741 tttttgaaca tggacatata taaccaatgt tcacagtaaa aaaagctgcc ttgcatcaag
     4801 taataacttc aaccaagata aaaataagag gagctatttt tgttgggagg ttcttattac
     4861 tgcggatgca agcttttgaa aactatttat tctagaaaac cgtttatttg aaatagggga
     4921 cggtgacaag gtttttgtca ccgcccctat tatttttgtg ttttaatagc tcttttttat
     4981 atagatagtg cccataccta tgaataataa aacgatactc catataccaa gtgtcaagat
     5041 accattcatg aaagtaacct gttctgaagc tagtggcgag aaaatatctc ctcctatgat
     5101 cacattttga aaatcaacat aagaagttgg caagtaatga ctgattgatt ctatccatcc
     5161 aatcgaagaa aacaagcgat actctgctaa gaaaagacaa agaagtgtac ctaaaagatt
     5221 gactagagaa tgttcaaata aagcagatag aagaaaactg attgtactta aaaagagcac
     5281 ccacaacaac aggaacagaa agctctggaa aagatactca tacatcggca ggattcgtat
     5341 ctcctgacca atgatcgttc ctacaggata acggaagtct cccaaaccat ttttgattat
     5401 tactagtata gagacgatca aaaatggcat cacgagactt agcatagctg caccataata
     5461 aatggtaaaa cggatgctat gtttctttac catactatcc agtaatagat tgactagaga
     5521 gattacctgc tttctttttt cataagagat gatggaagaa agaaacaagg cggttataca
     5581 taaaatcaaa aaactagaaa tcattcctga aaaaatattt tcgtagtaat ttgctaaaga
     5641 gagatgcgct ggcaagtcaa aaatagaata tctttggatc tgatgttctt tcatataacg
     5701 gagcaactcg acacgttttt gttgttcaat gattggaatt ccttgtcttt gaccagaaat
     5761 cagtcgatcc agtatatctt tttcataatg ataagttgct tcgaccattt gctggacatt
     5821 ccctgtgttt tttgcatgta agattgcttc gatcagtttg ttgcttttct ctaaactttc
     5881 cacaaattct ttctcattct catcttcttt caacgtttca atcaaacgtt gcatctcttc
     5941 atagttatga ttcagttcat cgaattctgt ataagcaagt ttatgatcaa aaaaaagtgg
     6001 gaatgcaaag agcacgacca acaatagagg aaataaaaag tttactttgt tttttagtaa
     6061 gagattgcgt tcaaatgtaa gtaaagacat agttacacct catataattt acgataaata
     6121 gctaaaatct cttccatatt tgaggattct gtgacgattt ctccattttt tagaaaacat
     6181 gctttagaac aaatcgattg gatattcgcg atagcgtggg aagaaagaag aatcgtcttc
     6241 ccctctttct gccattgtct gatgaggcta ttcaagatat caatacttcc tgggtctaat
     6301 ccgttcaacg gttcatcgaa taacatgaca gatgaatcac tgatcaagta agcagcaagc
     6361 aagacatgtt gtttcattcc tagagacatt tttccaatcg gcaaagtaag gtactcagtc
     6421 atcttcaatc gttggaaaac agtttggcat tctactttgc tctgccaata gtatttgatc
     6481 aaagtcaaat aatcaacagc cgatagatag tcggataact gattcgttga ttctagaaaa
     6541 aagaaagacc ggcgataatc tgtataagca gtaggagaga tgttgtgaag ggacaattga
     6601 tcataagttg ttggcagcag ttgggcaatg ccattcagca aagttgtttt gccaaatccg
     6661 ttcggggcaa tcaaagcagc tacttctcct gattctacta aaagattcaa ttgtcgaatc
     6721 acacgttttt taccacgttt catgttcatg tttttgatca gtaatgtcat agataggcct
     6781 ccaatataaa atttaataac ataatagaat ggaaaacatc taaattaaat gctaacaaaa
     6841 gttattgttt gcatattata aaaaaattaa aataacagtt ttatttaaaa ttgaagtttt
     6901 ctgataccga aatcttttta ttttgctgac atactcaaac ctttggcaca aagtttcgtg
     6961 ccaaagtacg actatttctc tttttagtaa gcggatacaa ttagagtatc aaaaggagag
     7021 agggagagac aaaatggaac ggacgattcc caaaaacaac atttcgctaa aagcgagggt
     7081 acaaaaatta ggcagtactt tatcaagcat ggtcatgcca aatattggag cactgattgc
     7141 ctggggtgta ttaacggctc tatttatccc agatggttat ttaccaaatg aggctttcgc
     7201 taccatggta agcccaatgc taacttatct gattccttta ttgatcggtt atactggcgg
     7261 aaaagtaatt gctggtgacc gcggatcagt tgttggggca attgcaacga tgggtgtgat
     7321 tgtcggaaca gatattccaa tgatgctagg cgcgatgatc atgggacctc ttggtggata
     7381 tgccatcaag aaatttgatc agctattcca aaaacggatc aaatccggtt ttgaaatgct
     7441 agtcaataat ttttcagcag gtcttatcgg ttttggatta gcgttattag gtttttctgc
     7501 gattggtcca gttgtcgatg cattgactca agcaatggca aaaggtgtag agatcatttt
     7561 atctgcccat ttgattccat tgacaagtat ttttattgaa ccagcaaaaa tcttattttt
     7621 gaataatgca atcaatcatg ggattttgac accattggga actgaacaag tagcagaggc
     7681 tggaaaatct attctattct tgcttgaagc aaatcctggt ccaggacttg gcgtattact
     7741 tgcttttact ttgtttggca aaggagcagc aaaatcttca gcaccaggag ccatgatcat
     7801 tcacttttta ggcgggatcc atgaaatcta ttttccttac gttatgatga aaccgttatt
     7861 attcttatca gttataggcg gaggaatgtc aggaagtttc attttccaat tgatggatgc
     7921 tggactaaga gcgccagctt ctccagggtc gatcattgcg attctagcga tgacaccgtt
     7981 caattcttac cttcctgtta ttttgggcgt gattgttgcc gcagctgttt cgtttggtat
     8041 ttctgcagta attttgaaag cggatgcgaa agatacaaat gaagacttcg aaaaatctgt
     8101 tcaagagaca aaggcagcta agcaagcttc caaaggtgcc caaggtattc ctcaaggagc
     8161 tacattctct ggcgtacaaa aaatcatttt tgcttgtgat gcaggaatgg gatcaagtgc
     8221 gatgggcgct tcgatcttga gaaaaaaagt acaggaagca gacttgcctc aaacggtgac
     8281 caatcaagca atcagccaac tgacagatga ctcgaataca ttgatcgtta ctcaggtcga
     8341 actacaagaa agagcaaaac aaaaagcacc gagcgcgcgt tttgtctcag tagaaaactt
     8401 tttgaattca cctcgatatg atgaaatcat tcaagaatta agcagcaagc agaaaacatc
     8461 agtttctgaa attgagaatg agccagcaga agagtttctt tcagcaaaca tcaaacaaat
     8521 caaagagatt ctacttgtcc acgatgatcg ttccggatca gcaacaatgg ggatgaccgt
     8581 gctcaaagat atattagaca agaaccattt aactatcccg ttaagaaaag tcaatatcca
     8641 agatttaaaa gatgaacctg attcactgat cgtagccaaa gaaagtttag cggaaaaagt
     8701 acagatggct gcacctcaat ccattcattt atcagtcaac agcatggtca atccgcaaaa
     8761 atacgaatcg attgcacaat atttgaaaca gacagcttaa gggaggaaaa cagcatgcaa
     8821 agtttgccat tcgacatgat cgagctcaat cgatctttta aaaccaaaga agaagcgatc
     8881 cgctattgcg gtcagatgct tgtagaagca gggtgcgttg atgaggaata tatcgatgcc
     8941 atggtagata gagacaaaat gttatctgtt tatatgggga attttatcgc gattcctcat
     9001 ggaacagatg aagcaaaaaa atatgtgaaa aaatccggca tctgtgtcgt tcaagtacca
     9061 gatggtgtag attttggtac cgaagaagaa gaaaagattg cgactgtttt gttcggtatt
     9121 gcaggagtcg gagatgagca tttgcaactt gtacagcaaa tcgcacttta ttgcagtgat
     9181 atggacaatg ttgtccagct agcagacgcg ctcagcaaag aagaagtaac ggaaaatcta
     9241 gcaattgctt aaggagagag aaaaatgaga gcagtacatt ttggtgcagg aaatatcggt
     9301 cgaggattta ttggagaggt tttggcagct aatggatttg agattacttt cgtagatgtg
     9361 aatcacacga tcattgatgc tttaaatgaa agaaaagaat acgaaatcga attagcagat
     9421 gaatcaaaag aaaaaatcac tgtaaaaaat gtcaaaggaa tcaacaatca aacaaatcca
     9481 gaaaaagtaa tccaagaaat cgctgaagca gatttagtta ctaccgccat tggcccgaat
     9541 atcttacctt ttattgctga gttgattgca aaaggtatcc aagaaagaga aaaggaagga
     9601 agcacaacat ctttagatat cattgcctgt gaaaatatga ttggcggttc agcatttcta
     9661 gaaaaagaag tgtataaata tcttccggaa acatcattta ctgacaaata tatcggcttt
     9721 ccaaatgcag cagtcgatcg aatcgtccca ttacaacacc atgaagatcc tttattcgtc
     9781 caagtggaac ctttcaaaga atgggtcatt gacgatagcc aaagaaaaaa taaaaaaatc
     9841 caattaaaag gggttttata tgtagatgat ctagaaccat atatcgaacg caaactattt
     9901 agtgtcaata ctggtcatgc gactgtcgct tatacaggtg ccttgttagg ttaccagacc
     9961 attgatgaag caatgcaaga tgcgttagtt gtggcacaat taaaatcagt tttgcaggaa
    10021 acaggaagtt tgctgattgc caaatggggg tttgatgcag agcagcatca tgcgtatatt
    10081 gaaaaaatca ttcatcgctt ccaaaacaaa aatatctctg atgcgatcac acgtgtcgct
    10141 cggacaccat tacgtaagtt aggttatcaa gaaagattta cacgtccagt gagagaacta
    10201 caagaacata acttgacttg cccacatttg acggcaacaa tggggattat ttttaattac
    10261 tatgatcctg aagatgaaca aagccgtcaa ttgcatgaaa tgaagatcca tgaaaacttg
    10321 gaacaattga ttcaagaagt cacaggaatc aatgatccga aaacaatcgg gaatatcaag
    10381 caaaacgtga accgttatgc caagcaagtc gcttaaaata ggatacgaaa aaggggaatg
    10441 tgacgaaatt tttgtcacat tccccttttt cgaataacta ggctgtttgt ccttcaagat
    10501 agggcagatc caattcttta ccgtaacgtt gttctaaagc taggcggatc aaacgagctg
    10561 ccaggttctc atttcttgca aggataggtc catggaaata agaaccgaag acatttcggt
    10621 aaatgacacc ttcagattgg tcttcaccat tatttccttg accttgtacc actttcccca
    10681 aaggtcgttc accttcacct aagaaagtcc gaccattgtg gttttcaaat ccataataag
    10741 tttcattgaa ttcttcgtta tggataacga tatcaccgat aaaccggtta ttttcctgac
    10801 ttaacgtgta gtgatccagt gcactgattc cttggatctt ttctccatta gctcccatgt
    10861 agtagtgacc tagcaattgg tagccaccac aaatggctaa catgacgcca tcattttcga
    10921 tatagttgat caaagaatct ttctttttct ggatgtcttt tgaaatgatt aattgctcaa
    10981 agtcttgacc accgccaaag aaaaccaagt catatttatc agcatcaaaa ggttcatgta
    11041 tactaacgat ctccgtatgg caagaaaccc ccattttttc tgctatatat ttcaacatca
    11101 aaaggtttcc gttatcacca tatgtgttca tcaaatttcc ataaaggtgg gcgatattga
    11161 gttcataatt agccacggtt cattcctcct ttgataaatc cttgagcagc tagttcttta
    11221 cgaagctgca atacagcagt gtaagtagct aaaatataga catgctcagt tggcaattct
    11281 ttgatttttt cgattacttc tggtaattct tttgtttctg tcaatttatt ttctggaatc
    11341 ccggcaactt ttagacgtaa agtcatatct gtatgacggt ctcctccaga aataacagct
    11401 gggatatcca tatcggcaaa gttttcaaaa ttaccatccc aaatccagct gacatcgatt
    11461 ccgtctgcgt aattggcatt taataaagcc actaaagaaa atggataagg agctagacca
    11521 atcatatcga ttacttgatt gatacctaca ggatttttga caagtactaa tgtacatttt
    11581 ttcccattga tattgatgac ttcttgtcgg ccgaatactt tctcgtcata tcctaaacct
    11641 tgtttgattt tatcaggatc gacattgaag tactctgcca ctgcagtagc tgctaaagca
    11701 ttgtagacat tatacatacc gccaacttcg attccgtatt catgaccatc gatcacgaaa
    11761 tctgctgaca cattgtccat ccgtaccata tcagttaatc gataatccaa ttcaggacgt
    11821 ttgaagtcac aatttggaca gtaatatttc cctaagtttg cataagtgat cattttataa
    11881 tgcaagatgt gatgacaatt tggacataag acaccgtctg tattgtaatg agccatttgt
    11941 tcacgatctt cttcatggtc aaagccataa tattttctag gattgactgt ttcgatagaa
    12001 ttgaagattg gtgaatcacc gttactgata atcgttgcat ttggtgactt tgcggcaccg
    12061 tctacaatca ttttataggt cgtatagatt tctccatacc gatccatttg atcacggaaa
    12121 atatttgtaa ataagaacag ttctggtttc atatattctg tgactttgct caaacttgct
    12181 tcatctattt ctaggacagc aaactttttc tgtccttttt ttggttttgc ctgcaagaaa
    12241 gtcgatacaa ttccctgtac catattcgca ccagtagggt tggttagaac ttcatcgaat
    12301 tgctggcgca aaatattgac tgttaaagcg gtagtcaatg ttttaccgtt tgttccggtg
    12361 acaacgacga cttggtagtc ttttgccaaa ctatctagta tatgtggatc gactttcagc
    12421 gctagtttac ctggtaaact tgatccgcct ttaaaaaaag tttttaatgc ccattgcgac
    12481 gtttttccaa ctgcaatcgc aaaatgactg cgtattccca ttatgtaacc tccaaaaacg
    12541 atttgctgac gaataaattc aactaatcat accatatgtt gaaaaaaagg ttaaattgct
    12601 tttcttattc ttaagaaaag gttacggctg aaagtgcatg aaatcggaca aaatcgcatg
    12661 atttaacaaa aaaacagctt gaaacaaaat gattgtttca agctgctgtg attttattat
    12721 tccctcttac ctatattccc attttaaccg ataataattt tctcgcttgg atattcatag
    12781 cctagatctc gtgtttcttt tggcacgaat aacatcaatg tatatagcat tccgatccgc
    12841 ccaataaaca tcagtgtagc aatcgtgatt ttgcccacaa cagacaagtc tccggtaatc
    12901 cctaaagata aaccagttgt tccaaacgca gaagttactt cgatgatgat cgagatcagc
    12961 gtttgttctt ctgtagcaga taaaaaaacg atacaaaaga aacacatacc aagagaaagc
    13021 ataaagacaa cgactgattt tcgcacatca tcttgatcga ttctccgtcc aaaaatattg
    13081 atgttatctt cacttttgag gaaggaatat agatacaaac cgatgattgc tacagtagtt
    13141 gttcgaatcc cgccgcctac agaactagga ctgcatccga taaacatcag taaagaaaaa
    13201 atgatcagtg ttgtgatttg aaaatcaccc aaatcgtgaa tctgcagacc agcattacga
    13261 gtcgtaatcg aataaaacat cgaattgatc catttgacac tcatattgga atcttggaat
    13321 aaatggtcct tttccaatag ataaatcaag acagttcccg aaacaaacaa gataacaaaa
    13381 gctaacacag ctaatttggt aaacaaggaa aaacgaaacg gaagcttcgc attggaacgt
    13441 ttgtataata gccattcccg gcactccatc aatacaggaa aaccaattcc tccgacaaaa
    13501 atcaaaaaca tgatcaggat caagaaaaaa tagtcatgtg caaagggctt gatggaatcc
    13561 ccagtgacgt caaacccaga gttcgtgaca gccgatatcg cctgataaaa accgtaaaaa
    13621 acggcatcac gccaacgatc aaaatagcct ctgtaataaa aataaataga aaagaatgtc
    13681 ccaaaaagta attgaaacca gattaaaata gcaaaagtga tccgaatcag tcggacgata
    13741 ccagacaaac gcggttgatt catatccgtc atgatcagct gtcgctgttt cagtgtgatc
    13801 cgtcgtttag aaaaaatgac aaaagcagta gaaatcatca tgatccccaa accaccaact
    13861 tgaaataata tttccagcag tataataccg ttatcattga acacagaatt gatatcaaaa
    13921 gtcgatagtc cagtcacact cactgtactg attgccatga acagcatgtc cacaaaagaa
    13981 acatggctat ctggttcgcg aaaaatcggc atataaaata gaacgagtga taagaccgtc
    14041 atcaatatat aataaaaaac gatgatttgg atcgatgaga aatgattggc agcaaacttc
    14101 tgtcctcgcc gctgcagcct tcgaaagaat aaatcaacat gcacagtact atcctcctga
    14161 caatagtcgt ttctaccagt atacaagggg aattaggaat actcaaagaa attatcaaac
    14221 agataacaat acagctttcg attaaaatag aaatggctta taataaggaa aatcctagaa
    14281 aaaagaagga ggaggaataa caaattgatt aatgctcaac aaatcgtaga aaaaatgaaa
    14341 aatcaaaaaa taaattatga tacagttctg aaaaaaatga tcatgcaatg ggagaaagaa
    14401 gaagtaaagc ctaagatatt aatacataca tgctgtgctc cttgcagtac gcacacattg
    14461 gaattcatgt ctcaatatgc agagatcacg ttattttttt caaattcgaa tattcatcct
    14521 aaaagtgagt atttgcgaag attacatgaa caaaaacgat ttgtagacca atttaatgag
    14581 aaaactgacc atcatgttgc gctaatcgtt gatgaatacc gtccaaatga attcaccaaa
    14641 atggttttaa caaaccatct tgaaaacgaa aaagaaggtg gtgctcgctg cagtgcatgc
    14701 ttcaatatgc gattagatct tgttgcgcaa aaagcgcaag aactaggata cgattacttt
    14761 ggaagtgccc tgaccatctc gccaaagaaa aatgcagctt tgatcaatca gatcggaatg
    14821 gatatccaaa agatttacgg gacacaatac ttgccaagtg atttcaagaa aaacaaaggc
    14881 tatgaacgct ctttgcaaat gtgcaaagag tatgatattt atcgccaatg ttattgtggt
    14941 tgcgtgttcg ctgccaagca gcaaggatgc gatctgaaag aaatcaatcg ggaagcaaaa
    15001 gcttacttaa aaacatcaac cgtagttttt gaggaaaatt aaagaaatct ttcggaaaaa
    15061 cgtaagaaaa aaataaagaa tttatggtag aaataaggta gactgtataa tttgtccgta
    15121 gtaaagtaag gaaacaagaa taaatttcaa tgaaggaaga gataagatgc gaaaagtagc
    15181 acctattttg aagccaatcg ctgtattaac aatctcgacg attgtcgcga ctactacagg
    15241 tattgtgaat agttccagct accgtgcacc aagttcaagt tctagtactg tagaagaaac
    15301 aacaccaact acttcagtct cgacatctga accaacaaca acggaaagtt tgaatgaaac
    15361 cactacgaca agtacatggg acgatacaac aactagttcg tcgtatgatg agacatatac
    15421 aactgacagc tcaagcgaaa caagtaatac ggtaccagat ggcactccat cggattcaac
    15481 caatccaagt acaagtccgg atacaagtgc tagctccgaa acgataccaa gcagtacgac
    15541 tgtagatgat aacacgcaaa atgatgatgc agcacgtaca gcacagcaaa accaagattc
    15601 aggaaatacc caaacgtata accagcaagc aggtgattct caatgacatt tttactagac
    15661 cttatcaacg gggtgcataa attccttggc tatttagaca tcagtcctaa atatcttaac
    15721 cgtggatata cgattttaag tgtgattccc acgctttatt tattgagaat tgtttatgga
    15781 ctttggcaaa accaaaacta tcttcaattt ttcttatatg gacttgcatt tctagtgctt
    15841 gtttatttta ctgttttaaa tgtattttat tactttttag ataaaaacac gaaagccgat
    15901 gtcactcagc tttttgtgaa gtatttacct gatgaagcgt tcaacatcca gacagaagaa
    15961 tcgatagata atggaaattc gatcgataaa gtgaatacac aagaggtcat ggtttcttat
    16021 gacgaagatt atcaattgaa attagccgag aatatgcggt atttgattgg aaatggtgag
    16081 atcaaaacaa atgaactcgg tcgcatggat ggatttctgg tcgatagaaa tacattgtat
    16141 ccttattact atgtcaagaa aacaggagag aaagagtatg ttctaaatat tggacgaagc
    16201 tactcggaca tggaaaaagt agggaccatt cgacttgact caacagaaga cacattggaa
    16261 cctgtcggat tgttcatcgt cggcggtgat tttgtcaaag acgggttacg ctatcatgag
    16321 ccttatcgtc tgaaattgat tgcaaaaaag gcacagtcgc aggaagaacc agtggtttat
    16381 agtcgtagcc aaagaagaag acattcataa tgtgatgaaa cgccgtgtat cagtggttgc
    16441 gataaaaacg tttattcgga ataaggggga tgtgacacgt cttttgtcac gttccttttt
    16501 tatataacga gaaagataag acgaacacat cacttattct ttcctttcat ttataaacat
    16561 tgtagaataa agaaagtaca acgaagtatt gtgaaaaaac gtaataaata acgaggaaaa
    16621 tacaagtagg aggtaagctg tgaaagaatt tttgaaaaat tgggggattt tgatccttgt
    16681 attagcggct attttattag ctagagtgta cgtgtttaca ccagttactg taaacgggca
    16741 ttcgatggac cctacattaa gtgatggaca acgattgatt tcatctaaga tctcaaacta
    16801 tgaacgaatg gatattatta ctacaaagga accgggggat gaagaacgga tgattgttaa
    16861 acggatcatc ggtatgcctg gagatacagt caagatggaa aatgaccagt tgaccattaa
    16921 tggcaaaaag tatgatgaac cttatctgga tgagttcaaa aaagaatttt cagaagataa
    16981 gttacaagga gaatatgctt atagctcagg attccaagca caagcagaaa gttcatccac
    17041 ctttacgagt gactttgaat atactgtgcc aaaaggaaag tatcttgttc taggcgataa
    17101 ccgcttgatt tcaaaagaca gccgaatgtt tgggttagtc gacaaagata tgatccaagg
    17161 aaaagttgtt ttccgttatt ggcctctttc agaaattaaa atcatgtgat gaaaaaataa
    17221 aaagaagctg tgacatgttt tttgtcacag cttctttttc cgaaaattcc ttcttatttc
    17281 ttggagttgc tcactttttt tgcaatctca ttttaagctt tttggctgat ggataagaaa
    17341 agcgaagcgt ttttgttcat aaatagcttt tccccacgat ggaaggtatg actagtagca
    17401 gagacaaagc ccgttttttc caatcgatta ttcaattctt cttggctaaa gccattatgg
    17461 actttcggat gactaatttg ttcattcttg tcaaagtcta cgatgatcaa ttgaccgcca
    17521 ggagctaaaa tttcataaag ttttgtaaga atattctccg tatcaggaat gtgtaaaagt
    17581 acaagtgata gaaggatgag attagcttgg acagcaggaa attcgacaga agcatcagcg
    17641 tggatcgttc ggacattttt cagatctgct gcttggatct tttcttctgc cattttcagc
    17701 atagtttctg aagcatcggc aatgatcagt tctttgaatc tttccgcaag cggtaaacta
    17761 acaagcccag ttccgcctcc gtaatccagc aggacttttt ggtggctgtc gcgcggtaga
    17821 taagtagtta gttcttcgcg aataatcttg gctaatgctt gacggtcagg ggaatcataa
    17881 tggttagcga tggaatcaaa aatatttttt gtcatcaaat agctccttac gtaaaacttt
    17941 gaaattttaa cttattatag cacgtgaaaa gaaagaaagc atgctatact caattcaaaa
    18001 agggagaatc tagaaagaat atgccgatat cgtttgtaaa agatagagag gaaaaaggaa
    18061 agtgtgtccg agaaatatta ctagacttgc ctgagtggtt tgggcttcct gaatcaacgg
    18121 aaaaatatat cgaggaatca agtaaactac ctttatggtg tgaaaaaaga aaagaagaat
    18181 atttaggctt tatcactctt tcacagacaa gtgaagatac tgccgaaatc tattctattg
    18241 tatgggaata aaaaaagact atcatcacca aggattagga acaaaacttt ttaaagaagc
    18301 agaaggttac gcagcaaaac attacaaata tttgcaagtg aagactgtag acgaggggca
    18361 ctactctata tatgatcaaa ctatttgttt ctacgaatca ttaggatttt caaggttgga
    18421 agtttttcca aatttatggg atgaatggaa cccatgttta gttcttgtca aaaaattgga
    18481 acaaaagtga gcaatagatt taatactaat ctgtgcttga tgaaaattct cactgtaaaa
    18541 aatgctgaaa atcaagtata ataccatagg aacaaactgg aaggtgaaaa agagcatggc
    18601 acaactattt tttaagtatg gcgcaatgaa tagcggcaag acgatcgaga tcctaaaagt
    18661 cgcacataat tatgaagaac aaaataagcc agttgtctta atgacaagcg gtattgatac
    18721 aagagatggt ttaggcgttg tttcaagccg aatcggactg aaacgtaatg cgatgccgat
    18781 atttgaagaa acagacgtat ttgacgtaat tcagaaaatg gaaagcaaac cctattgtgt
    18841 gctgatcgat gaagcgcaat tcttaaaaaa gaaacatgtc attgctttta cgagaattgt
    18901 ggacgaattg gatattccgg ttatggcatt tggtttgaaa aatgattttc gaaacgagtt
    18961 atttgaagga tcaaaatatt tattgcttta tgcagacaaa atcgaagaaa tgaaaacaat
    19021 ctgttggttc tgccacaaaa aagcaatcat gaatttacat tatattgatg gaaaccctgt
    19081 ttatgaaggc gatcaagtgc aaattggcgg aaatgaagct tattaccccg tttgtcgaaa
    19141 acattacttt cacccatcaa tgattacaga aggagaataa gcatgtacga tcaattacaa
    19201 gcaattgaag accgttatga agagcttgga gaacttttaa gtgacccaga agtgatttct
    19261 gatacaaagc gttttatgca gctttcaaaa gaagaagcaa acacgcgcga aacagtcgaa
    19321 gtttatcgtc gttataaaga agtagtacaa ggaatcaccg atacagaaga actgcttggt
    19381 gaaaaactgg atgatgaaat ggctgaacta gcaaaagaag aattatctga attaaaaaaa
    19441 gaaaaagaag tcttagaaga aaaaatcaag atcttgcttt taccaaaaga tccaaatgac
    19501 gataaaaata tcatcatgga aatccgtgga gcagcaggcg gagacgaagc agcattattc
    19561 gctggcgatc ttttcaatat gtaccaaaag tatgctgaag cacaaggctg gaaaacagaa
    19621 gtgatggaag ctaacgttac cggaatcggt ggatataaag aagtgatcat gatgatcacc
    19681 ggagacaacg tatattcgaa attaaaatat gaaagtggtg cgcaccgcgt ccagcgtgtt
    19741 ccttcaactg aatcgcaagg gcgtatccat acttccaccg caacagttgt cgtgatgcca
    19801 gaagctgaag aagtagagat cgatattgaa gacaaagata tccgtacaga tatctatcat
    19861 gcttcaggtg ctggcggaca gcacgtcaat aaaacagcat ctgctgtacg tttgactcac
    19921 ttaccaacag ggatcgttgt tgctatgcaa gatgaacgtt ctcaaatcaa gaaccgtgaa
    19981 aaagcaatga aaatcttgcg tgctcgtgtg tacgataaga tccagcaaga agcacaaagc
    20041 gagtatgatg ccaatcgaaa atcagcagtc ggaactgggg atcgttcaga acggatccgg
    20101 acgtataatt tcccgcaaaa tcgtgtgact gatcatcgta tcggattaac tatccaaaaa
    20161 ttagatcaga ttttagcagg gaaattagat gagatcgttg atgcattgat catgtatgat
    20221 caaacatcaa aactggaagc tatgcaaaat gggtaaaatg acctatcgag aagtcctgtc
    20281 acgggcttct tcttttttag aggatcatgg gaaagaagca tatagtatcc aatttttatt
    20341 tttggaacga aaacaatgga aaaaacttga ttggctcctt catatgaacg aagagatttc
    20401 agaagaagaa cagcggttga tcgaaaccga tctgcgactt ctgcttgcag atcatccacc
    20461 tcagtatttg ctaggatatg cagattttta tgatcatcgg ttaaaggtaa ctgaagcaac
    20521 actgatccct cgaccagaaa cagaggagtt agtcgaatgg tgtttagacg agacgcctgg
    20581 tgtaccgtta gaagtgatag atattgggac agggactgga gcaattgcta ttagtttgaa
    20641 agcagcaaga aaaaattggc atatatctgc tgtggatctt tcagaagaag cattagaagt
    20701 agcgaaagaa aatgctcaga aagaaggaac gaaaatcagt ttttatcatg gagatacatt
    20761 aggaccagta atggatcagc aatttgatgt gattatttct aatccgccgt acatcagtcg
    20821 gaatgaatgg gaactgatgg atgaaagtgt ccggtcattc gaacctaaaa tggccctttt
    20881 tgctgagaac gatggattag ctatttatga aaaaatagca gttgaagcac caagcgtact
    20941 aaaatcaaat ggaaagattt ttttagaaat cggatttcgc caaggtgaag cagtaaaaaa
    21001 aatatttcag caagcatttc cagataaaaa agtggcgata aaaaaggatc tttttggcaa
    21061 tgaacgaatg atccaagttt atggttgaag ttgtggatga aaaatttttt atttgttttt
    21121 ttattataaa aaaacactta tttgacaagg atgtaaacga ctttttggtt tatcaacaag
    21181 tttatccact attgacaaat agattatcca caaacagtgg aaaacttgag gataaactca
    21241 gacaaaataa gaatgtatga aaaaggggtt gtggataaac tgtggaaacg aaacgatatg
    21301 atgaaacaga attgcaagag gcagccgaag cactgaaaga aggagaactt gttgcttttc
    21361 ctaccgaaac agtatacgga ttgggcgcca acgctctttt accaaacgct gtcaagaaag
    21421 tgttttctgt aaaagggaga ccgcaagata atccattaat cgtccatgtc gcctcttttg
    21481 aacaagtaaa agagtatgtg gacaactttc atccggcagc agagaagatt gtgaagaact
    21541 tctggcctgg accattaact ttgatattta agatcaaaaa agacactttg ccctcagttg
    21601 tgacgggagg attatccaca gctgcatttc gtataccgga taacaaaaaa acactagaag
    21661 taatcgaact ttccggtgtt ccgcttgtcg gtccaagtgc aaatacgtcg ggaaaaccaa
    21721 gtccaacgac tgcagatcat gtatatcatg atctacaagg aaaaattacc gggattattg
    21781 atgatggagc tacgcgaatt ggtgtagaat ccactgttct tgatttaagt gatccaacag
    21841 ccatgcctat gattttacga ccaggtgcag taacgaaaga acaaatcgaa gcagtcatcg
    21901 aatctcctgt tgctattgat cagcatcttg taaaagaaaa tgaaacacca aaagcccctg
    21961 ggatgaaata caaacactac tcacccgata cacgtgttct gatggtcaga gatggcgatt
    22021 ggtcaactgc agttcaatgg gcaaaaaata agaagatccg tgcaggtgtc atagcaagcc
    22081 ccgaaattgc ggatcaagtg cggacagata ctgcagctgt ttatatgtat aacgacaact
    22141 cagtagaagc ggcggctaaa ggattgtttg caggtctaag aggattagat gaaccaacac
    22201 ttggattaga tttgattttt gtacaagtgt atccagaaac tggattgggg aatgcttaca
    22261 tgaatcgctt gaaaaaagca gcaggacaaa attattttga aaaatgatca attggttatt
    22321 gtcgcattct tttagtatga tacaatgcta tcaagtaaat cttctatcag tgaacgaaaa
    22381 actaagcgta actaatcgta atagaagaga tacgaatatt tggcaatttg tttttgatgg
    22441 gaaaaataaa attgtaggaa aataaaattg taggaaaata aaaaggagct gttgtttgtg
    22501 gtagattaca aaacgtttga ccctgatctt tgggctgcga tcgccaaaga agaagaaaga
    22561 caagaacata atttggagct gattgcctct gaaaactttg tctccgaagc agtgatggct
    22621 gcacaaggaa gtatcttgac aaataaatac gcagaaggtt atccagggca tcgctattat
    22681 ggtgggtgtg aatttgtcga tatcgttgaa aatttagcga ttgaccgtgc aaaagaatta
    22741 ttcggagcga aatttgcgaa cgtccagcct cactccggct ctcaagccaa cacggctgct
    22801 tatctcgctt tggtagaacc aggggacacg atcttaggaa tggatctttc cgcaggcgga
    22861 catctaacac atggatcacc agtcaacttc agtggcaaaa cctatcactt tgttgcttac
    22921 ggtgttgatc cgacaacaga agttattgat tataatgtgg tacgtatttt agctagaaaa
    22981 catcaaccaa aattgatcgt agcaggtgcc agtgcttacg gacgaacgat cgactttgcg
    23041 aaattcagag aaatcgcaga tgaagtaggc gcaaaactga tggtagatat ggcacatatc
    23101 gcaggattag tagcagcagg acttcatcct aatccagttc cttatgcaga tatcacaacg
    23161 actaccactc ataaaacatt gcgtggccct cgaggcggca tgattttgac aaacgatgaa
    23221 gcattagcaa aaaaaataaa tagtgcggta tttcctggta ttcaaggcgg ccctctagag
    23281 catgtgattg cgggtaaagc agtagcattc aaagaagcat tagatcctgc atttaaagaa
    23341 tatagtgaac aaatcatcgc caatgcaaaa gcaatggtca aagttttcaa tcaagcaatt
    23401 ggaaccagag tcatttcagg ggcgacagat aatcatctga tgttgatcga tgttcgcgag
    23461 ttaggtataa atggaaaaga agcagagagc attttggaca gtgtgaatat cactgtaaat
    23521 aaaaactcga ttccttttga aacactaagt ccattcaaaa caagcggtat tcgaatcggc
    23581 acgccagcaa tcactactcg cgggttcaaa gaagaagatg ctgtaaaagt ggctgaacta
    23641 gttgtcaaag cattacaagc aaaagatgat aacgctcaac tagacgaagt caaaacaggt
    23701 gtacgcgaat taactgaaaa atttccgttg cataaaaaat aaaaatttaa attccattag
    23761 taaacagaaa agttcaatgt tgggcttttc tgtttatttt ttgggaaaac ggattctttg
    23821 acaatatcgg tgatgctctc tagtatatga ggtgtaattt tagtcttcac actaaatctc
    23881 agggaggaaa agtcatgact gatcgtatct cgttagaaaa ggaagcagta gcattcagtg
    23941 atgcaaatgc gcctcatccg cgtatctatg aactagcacc ggaagaagga agagttttac
    24001 tagaagaagt acaagaatca cctgtgaaca aatattccgt cgatattgaa gacttgatgg
    24061 tggatactgg cgaatgggga aaactcaata ttcgtctcgt acgaccagaa gggaatatgg
    24121 ataaattacc agttattttt tatattcatg gagcaggctg ggtttttggc agtgctcaaa
    24181 cccatgataa attgatcaga gaacttgcgg ttcgtacgaa ttccgtcgtt gtctttccag
    24241 aatatagtcg atcaccagaa gcaaaatatc caacagcaat tgaacaaaat tatgctgtgt
    24301 tgcaacagct gaataaatta gccaagtcaa aaaatctgga tctcgagaat ctaatcgttg
    24361 caggagattc agtaggtggt aacatggcta cagtcatgac gatcttaaca aaagaacgaa
    24421 agggcctccc aatatccaag caattgctgt attatccagt cacaaacgct gattttgata
    24481 cagattccta tcatgaattc ggagaaaact attttttgac aagagaagga atgatgtggt
    24541 tttgggacca atatacgacg aatgaagaag agcgtgctga aatcacagct tcgcctttga
    24601 gagcaaatat aaatgaacta aatgatcttc cttcagcaat gattttaacg ggagaagcgg
    24661 atgtattaag agatgaaggt gaggcttatg cgcgcaaact tcgtgatgca ggggttcctg
    24721 ttacacaagt acgcttccaa ggaatcatcc atgattttgt catggtaaat gctatggatc
    24781 agacaaaggc aacgcgtgca gcaatggcat tatctacttc ttggatcaag aaaaaagaat
    24841 aagctttgct tgtccgcagg aaaacttctt ttcaaaaacg gattttctta ggaaaaatgg
    24901 cgagtttgac tagtcaatca cttgattttg ttttacaatt aaaaagaaac gacagaacaa
    24961 aggagagctt aaaaaatggg caaatttcaa gtgatcgatc atccattgat tcaacacaaa
    25021 ctaactatta ttcgggacaa aaactgcgga acaaaggttt ttcgtgaagt agtcgatgaa
    25081 atcgctatgt tgatggcata tgaagtatca cgggatatgc cattggaaga catcgtgatc
    25141 gaaacaccga tcactgaaac aacacaaaaa acgttgtctg gtaaaaaagt agcgattatt
    25201 ccaatcttgc gcgcaggtat tggaatggtt gacggtatct tggaattgat tccagctgct
    25261 aaagtgggac atatcggtct ttatcgagat gaagaaacat tggaaccaca tgaatatttc
    25321 gtcaaacttc cagaagacat tgatgcacgt cagttgtttg tagtagatcc aatgttggca
    25381 acaggcggat cagcaatcat ggcaatcgat gcattgaaag aacgtggcgc gtcgaacatt
    25441 aaatttgttt gtttggtagc agcacctgag ggagtaaaag ctttgcaaga agcgcatcct
    25501 gatattgaca tctatacagc ttctctagat gaaaaattga atgaacatgg ttatatcgtt
    25561 cctggactag gggatgcggg tgaccgtttg tttggtacaa aataagtaaa tacgaaaaag
    25621 ggtgtgacac aacttttgtc acatcctttt ttggtcattt ttaacgtttg atgcagaaca
    25681 gctttttcac agctttcgtt tgcatataaa aaaaccatcc caaaaatgca gagatggtca
    25741 aattcaaatc ttttttagtt ctttgtagtt aacgattact acacccttac tttgcgtcat
    25801 caagttcgca ttttctgcag tttcatcaat ctcaacaaca gcggaatttt tcaattgctt
    25861 tgaaatcgtt ccagtttgcg acttgccgtg aattgtgaac attacgtgag tattgatctt
    25921 gaatttttca ccaatttccg atggtacgat agttgtatca aattttccaa aagttgccat
    25981 tttcataaaa cctgcctttc acatcaatta taccacgttt tatgaaaaat atcatgaaat
    26041 taaactaatt ttccatcgga ctctttacaa tcagccaaat agagtatata atttcttttg
    26101 gataaggcca gcttagctca gttggtagag caacgcactc gtaacgcgta ggtcgtaggt
    26161 tcgatcccta tagctggcat taaaagagaa gacagatata tcaaggattt ttaatccatt
    26221 gatacatctg tctttttgtt taaaggaaat aaagtatgct ttcttagcaa tagagcatgt
    26281 attatttcta taaaaattta ggagaataaa acgactagca atatagctgt gaaatcaaat
    26341 atcacatgaa ctgcccaact tgtaatgatc gagttgcttt taatggcagc ttggttgaat
    26401 aacaatcttg ccaccccttg tatcaaaagg atgtgtgcta cagtgtgtaa aatatttcca
    26461 ttatcataag tactgtaatg gataaatcca aagatgattg ctgacaagaa actagcgaca
    26521 ggaatgctga acttactaga aaaaatagcg agaaaataca ctgagaacaa ttcttctcca
    26581 agtatcatga taggcaaaat aataaataat aacgggctga aagcctcatt tattggattg
    26641 cctgttaaac cccatttcaa aatttgttgc aaaaataccg agactgcggc actgattact
    26701 acattgataa aaaaatattt ggctatattt ttgacaggag cgacaggttt cgagaacatt
    26761 tttttcgtat cttttttacc aaaaacaagt gtaaatgcta taaatccagt gatagttaaa
    26821 gaaaaaatgc taataatgga tggcgcaaat agcaacccta acaaaccgag tacaacgaca
    26881 atagtgccca atatcccttt tttaagttct attttttttc ctaaaatgtt attcatgata
    26941 ataactccta ctattcagt
//
LOCUS       DASJTC010000032        26863 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.032, whole genome shotgun sequence.
ACCESSION   DASJTC010000032 DASJTC010000000
VERSION     DASJTC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.032"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..242
                     /locus_tag="U4B19_001221"
     CDS             <1..242
                     /locus_tag="U4B19_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HEN2076973.1"
                     /translation="RKNSRVKLRISFEKNTKRIVGVQLCSQEDLSGLLSMFSLAIEEQ
                     ITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            354..1805
                     /locus_tag="U4B19_001222"
     CDS             354..1805
                     /locus_tag="U4B19_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEN2076974.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            2154..2786
                     /locus_tag="U4B19_001223"
     CDS             2154..2786
                     /locus_tag="U4B19_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEN2076975.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3374..4813
                     /locus_tag="U4B19_001224"
     CDS             3374..4813
                     /locus_tag="U4B19_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2076976.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            5108..5326
                     /locus_tag="U4B19_001225"
                     /pseudo
     CDS             5108..5326
                     /locus_tag="U4B19_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5575..5790
                     /locus_tag="U4B19_001226"
     CDS             5575..5790
                     /locus_tag="U4B19_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076977.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(6007..6263)
                     /locus_tag="U4B19_001227"
                     /pseudo
     CDS             complement(6007..6263)
                     /locus_tag="U4B19_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6450..7361)
                     /locus_tag="U4B19_001228"
     CDS             complement(6450..7361)
                     /locus_tag="U4B19_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2076978.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7380..8384)
                     /locus_tag="U4B19_001229"
     CDS             complement(7380..8384)
                     /locus_tag="U4B19_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEN2076979.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8381..10504)
                     /locus_tag="U4B19_001230"
     CDS             complement(8381..10504)
                     /locus_tag="U4B19_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2076980.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10509..12956)
                     /locus_tag="U4B19_001231"
     CDS             complement(10509..12956)
                     /locus_tag="U4B19_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2076981.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12943..13332)
                     /locus_tag="U4B19_001232"
     CDS             complement(12943..13332)
                     /locus_tag="U4B19_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2076982.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13392..13895)
                     /locus_tag="U4B19_001233"
     CDS             complement(13392..13895)
                     /locus_tag="U4B19_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEN2076983.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13908..14132)
                     /locus_tag="U4B19_001234"
     CDS             complement(13908..14132)
                     /locus_tag="U4B19_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076984.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(14236..16146)
                     /gene="ltrA"
                     /locus_tag="U4B19_001235"
     CDS             complement(14236..16146)
                     /gene="ltrA"
                     /locus_tag="U4B19_001235"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2076985.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(16892..17029)
                     /locus_tag="U4B19_001236"
     CDS             complement(16892..17029)
                     /locus_tag="U4B19_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEN2076986.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(17026..18210)
                     /locus_tag="U4B19_001237"
     CDS             complement(17026..18210)
                     /locus_tag="U4B19_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN2076987.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18469..18759)
                     /locus_tag="U4B19_001238"
     CDS             complement(18469..18759)
                     /locus_tag="U4B19_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076988.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18749..19891)
                     /gene="dcm"
                     /locus_tag="U4B19_001239"
                     /pseudo
     CDS             complement(18749..19891)
                     /gene="dcm"
                     /locus_tag="U4B19_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19967..20131)
                     /locus_tag="U4B19_001240"
     CDS             complement(19967..20131)
                     /locus_tag="U4B19_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2076989.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(20124..20798)
                     /locus_tag="U4B19_001241"
     CDS             complement(20124..20798)
                     /locus_tag="U4B19_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2076990.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20876..22216)
                     /locus_tag="U4B19_001242"
     CDS             complement(20876..22216)
                     /locus_tag="U4B19_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEN2076991.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22299..22634)
                     /locus_tag="U4B19_001243"
     CDS             complement(22299..22634)
                     /locus_tag="U4B19_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEN2076992.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22644..23018)
                     /locus_tag="U4B19_001244"
     CDS             complement(22644..23018)
                     /locus_tag="U4B19_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2076993.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(23031..23345)
                     /locus_tag="U4B19_001245"
     CDS             complement(23031..23345)
                     /locus_tag="U4B19_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEN2076994.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23458..26685)
                     /gene="fss3"
                     /locus_tag="U4B19_001246"
     CDS             complement(23458..26685)
                     /gene="fss3"
                     /locus_tag="U4B19_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEN2076995.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         7824 a         5569 c         4675 g         8795 t
ORIGIN      
        1 tgagaaaaaa tagtcgtgta aaattaagaa ttagctttga aaaaaataca aagagaatag
       61 taggtgtaca actttgtagt caagaagatt tatctggact attatcaatg ttttctcttg
      121 ctatagaaga acaaattaca ttagacaaat tgaaaatgtt agattattta tttcatccta
      181 gttttagcca accctataat tttattttaa aggcagtaac aggattaaac attgaaacat
      241 aatcgttact aaatgaaatc aagaaatata ctgtattact caattcattg aaaatattta
      301 tcataatttt taatagaaaa cactattaaa gagaaaaaag agaggtaaat caaatgaaaa
      361 aatctttaaa acttgcatca gcagttatat taatgtccca actccttata gcaacacctt
      421 tccatgtgtt ggcaagcgaa aattcaacat acaaaattgc aaaagaaaga accgagacga
      481 ctgaaatgag taattccgat gtaacagcag agagtagtga acttttgaca acttcatcaa
      541 gtgaaagtag tacgacttca aatgccgagt cttcaacaac ttcatcaagt gaaagtagta
      601 cgacttcaaa tgccgagtct tcaacaactt catcaagtga aagtagtacg acttcaaatg
      661 ccgagtcttc aacaacttcg tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa
      721 caacttcaag tagtacaagt gaaataactt cttcgaaagt gacaaaacca aaccatatag
      781 aatcttcaaa aaaggaagcg ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac
      841 agcaaataga atcaacagaa tctttccaat cacaaccaat actatctggt aatgaagaaa
      901 ttcattttga caaaaatgaa tctgtagaaa attttattat gaaaattggt gagtctgcga
      961 gaaaaattgg tcagaaatat gatttatatg cttctgttat gattgcacaa gcgatcttag
     1021 aatctgccag tggtcaaagc cagttagctc aggcaccgaa ttacaattta tttgggataa
     1081 aaggaactta taatggaaac tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa
     1141 cgctttatac gacccaatca aaatttagag tgtatgaaaa ctatgaggaa agctttgaag
     1201 attatgccaa attattaact aagggaatta gtggaaataa agatttttat gcaggagcat
     1261 taaaagctaa tagtaaaaca tatcgagaag caactaaatt ccttacagga cggtatgcaa
     1321 cagatacaca atactatttg aagctaaatg aattaattaa aacctacgat ttaaccaact
     1381 atgataaaga agttaaaggt ccacaaatga gtaagactgg ataccaagtg cctattcata
     1441 attattcaat aaccagtttg tttggaaatc gtggaaatga atttcaccga ggacttgata
     1501 tggcagctac acagggtgag ccgattcatg cagttaaagc tggtaaggtt gttaaagcag
     1561 agtttcacta ctcttgggga aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt
     1621 tatatgctca ccagcaacaa tacctagtaa aaactggaga taatgttttg caaggacaaa
     1681 taattggcta tgtaggaagt actgggaata gtactggccc acaccttcat ttggaaattt
     1741 gcaaagatag tagtctttct cagagcatgt tagttgatcc gaaaacctta ttattcggag
     1801 actaaaaaag gtaatcaatc tgacaaagta acaagaaaat ggttggattc ttagaccttt
     1861 gatcaggata ttgagactgg aattgatatc tcgattacga tgggtttgac aaataaaaca
     1921 agtccattat cgaatatcga gtgtattatt gccgtcttta tgctcatatt ttgagcaggt
     1981 ttcactagat gaaaacaatt tatctatctt gaggatttcg tgtacgtatc gttcagtctt
     2041 gtattgtaaa ttagtcataa aatcagacta agaaaaattg gaaatatttt tttctgattt
     2101 attaaaagtt aattgaaatt atatggtact tctttcttgg gagggtaggt gcagtgaagg
     2161 aaagatatgg tcaccttact gttgttgagg agattactca taatggatat aaaaaatatc
     2221 gttgcctatg tgactgtggg aatgaaacga ttgtatatcg atcaaactta ctatcaggta
     2281 gaaccaaaag ttgtggatgt ggaaattata aaaataggca taaatataaa aatttgacaa
     2341 ataaaacttt tggtaagtta gttgcaatca agccaactaa actgcgaagt gaaggaactg
     2401 ttgtttggga atgtcaatgt ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc
     2461 taaaaggtga tgtaaaaagc tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata
     2521 aaaaattcgg attacttact gcaaagtatc ctttggacaa taactgttca tggaaaacaa
     2581 aatgggtttg tcattgtgag tgtggcagta tgtgtgagat aagttatagt aacttatttt
     2641 ttggacatac tcaaagttgt ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag
     2701 ggacagtaat agagtgtcta acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg
     2761 gagtttatcg ttctaaagag aaatagattg cgtatataac tttaaaaaaa aagaggtact
     2821 atttaggatc gtatgattct attaagagtg cagctcaagc tagaaaagat gctgaaaata
     2881 aattgtttca acctttgatt gacaaatatc gaggtaatca ttaattattg tggataataa
     2941 ttaacagtga tatataaaca tctataatat attattcata ctattgatat ttagtataat
     3001 cctatggaat tgaatatcat agcaatgtat ttgaaaagca aattggaaaa caaataggtg
     3061 caacaccata aatatgaaaa aattgggaaa aagtagaatg gaaatttgct tacgtctcac
     3121 gagatagctc acactattaa tctgaagaac aatgaagtcg acttttagaa attgctagaa
     3181 gaaagtaaag cgaattatca ctagttacta tagagtaagt tcaaataaag catctccaac
     3241 ttacatggtt acatgaggat atcaaatcga aattactcaa gcttcctatg gctagagtcg
     3301 cgagaagttg ttgggcagta gacataggtt atattgttct tagctacacg atcattagaa
     3361 aggaaatcct attatgtata gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg
     3421 gattttgatt gctatatcta tgtttacctt catgtccaca ttggatggca gtattgtcaa
     3481 tattgcacta cctaccatct cagatgatat gggcatacca atgaatcaag cggaatgggt
     3541 agtgtccgtt tatctgatga ttgtctgttc ttgtttgttg ctgtttggta aaattggcga
     3601 tagccgtggg aaaatcaaag tatttcggat tggtacgctg atctttgtct tgggatcgct
     3661 gttgtgtggg ttcaatcatt cgctaggtat tttattgttc gcacgggtgg tacaagccat
     3721 tggtgctagt atgactatgg ctacaaatgc cggaatcatc acggaagtct ttcccataca
     3781 tgaacgtggt cgggcattgg gagcaatagg tgcgtttgtt tcattaggct cgattgctgg
     3841 accaggtctt ggcggcttga ttttgtccca attttcttgg gcgtacattt tttggatcaa
     3901 tgtaccggtg ggaatcgtaa cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac
     3961 catgagcggc catacaatcg atggaggggg ctttgctgcc attgcggcta cgattatcag
     4021 cttttttgga gcaatctttt tggggcaaga aagtggcttt ttccatccag tcccccttgt
     4081 actgttcgta atttcagtga ttgcctttgt cagcttttta aaagttgaaa agaaagcagc
     4141 ctctccattg atttctttcg gtatttttaa gaacaagatt tttaccatga gcttgattac
     4201 agccttgttg attttttctt ccaacttctt tgtcaatgta gtgattcctt tttatctgca
     4261 aaatgctcgc gggttgagtc ctagctatgc tgggctgttg atgatggtct ttcctttgat
     4321 gatggtcgtc ggggcgcctt taagcggcta cctgaccgac cgggttggcc cggagatttt
     4381 ggtattagta ggcttgggct tattgtcggt aacccaggtc atgtatatgt ttttgtcagt
     4441 ggactctcct ttgtggctat acattgtcgc tactgccatc atgggtgccg gcaatgcctt
     4501 gttccagtcc cccaataata cgatggttat gagctcggtc actatcgaaa acttgggact
     4561 ggcaggctcg atgaattcct ttgcccgaaa tttggggatg gtcagcggga tttcgctttc
     4621 tacgacggtc ttatatcaag cgatgtctgc taagatgggc cagcaagtaa ctaactatat
     4681 cgcaaatcgg ccggatgtct ttttatatgg aatgaagatt acctttttag gcagctttgt
     4741 tttgtgtttt gttgcctttt tcctgactat ctggcgtatc aaaaaacaag gaggtattca
     4801 gaaaaatgtc tgaaaaaacc actgttgaag aatttttgga ggcctgcgta tgggtgagtg
     4861 ggatttccat ggatgatgga agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt
     4921 atcttcaaaa tactagtgct caatgagatg cttgatttga tcacaagaag actgcgaaag
     4981 ccgctggcaa ccgcaagcta ggtatcccac cgatggacta cgttttagag gctactggtt
     5041 atgaatacgg agccaacgct ctcatctgca taagaataca tcacccagat tatgtaaaga
     5101 ttttcgatac ttctgtcaaa aaatcattaa gatagataaa tgatttccgt ctagtcaaat
     5161 tggttcagaa tgtagacaca cagatggtaa gaagtcactc acaattcgag aatggacttg
     5221 tcctatttgc cgtatacatc ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa
     5281 gggctatgaa tacaagcatt gccttagaaa acatcaagaa ccttaggaac aacggggata
     5341 gcttggttaa taagagatac cgctgtttag gcttgcaagt atactctttt atcaagaatt
     5401 ccgtggcttt agtcatgaga ggcttaatac tatgaaaatt ttctttagca tttttgattt
     5461 aggttccttt tctaattata ataagatgac cactacagta acctactgaa atatataaac
     5521 agccactgta gtaccatttt caaagtagtc tatgagagga gaatcttcat tcttatgaac
     5581 gctggaattg cggatatgaa tttaataaaa aaaaccctta atgattttac ttccaattca
     5641 atatcaaaag gtactggtat caatttaagc acaataaaaa agctgaaaag tggtgaaaga
     5701 tcagtggaaa agctgaatct tttagatgct ataaaaataa cggaatttgc tatgaaaaat
     5761 ggaaaagctg agattgaaat atggagataa ttatcgtcta gtgaattact tgccacttag
     5821 ctgaatttaa cgatccttgg tgcttgaagg gggagattct tgggaataga gcatacttgc
     5881 aagcctatgt ctttactaac aacgatatct cttattaacc aagctatccc cgtagttcta
     5941 aggcttttac ttttatctaa gcaataatcg tattcaaagg aaatttttga cccttagaaa
     6001 gtctcatttt atcagacgtg agcttaggca ttgctgaatt ttgttaattc ggttttaggt
     6061 tgtgctttga gcataatatg aaagtattct ttatcacgat ttcattcatc taaggtaatg
     6121 tgatgtgatt ctgaaattct ttcaaaggca gtttttgcat aagctgacat tacatcatca
     6181 atgacatgtt tacaatgttt cactactaaa ataagggggt aaccaagaag aaaaactgaa
     6241 tgattatttg tatctaattc catggtatat caaccatttc ctatatagac tgattatgac
     6301 atggattaaa ataaaaaggc actatgtctt aattgtcggt tttcgagtta ccgaatggca
     6361 ttcatcaccc acctatagag gatgggcgac ttctgcctga aataagttac actacttata
     6421 acttctaata aaattagatt gagtcattat tatttaacat caacgatttt ccagtttcca
     6481 tttttttgta atactaattt gtactgaaag tagctagtgg tttttgatac attatcaagg
     6541 tattttatct ttgtactcac tagaaaattt tttccgtttt gtagataaac tggattctct
     6601 aactccgaaa actttaagtc aatattgatt ggtcgcatcg caccattttc aacataatac
     6661 tccaattctt tttcagaagc tgatggataa agtttaaaaa atgtttctaa aaaatcggtg
     6721 atttcttccg ttgttttggc atcaatttct gaatcgtttt ctacttgttt tgcttcatat
     6781 tttgccttgt ctggctttcc aatcattgtt ggcaaactag ttactaaatg attcctgttt
     6841 tcatcttcaa agattgtcac tcgatatgca gaagaaattg attgcgtctt ttttccctct
     6901 gtaatatgtt gctccactaa aaatgaaacg acaaattctt ccgagtgttt ttcaacatct
     6961 agaattttta cagattgaac ttcggaactc gtgggaatat ctgctcgaac catatcttgg
     7021 attaaagcga gaccgttttc tgtaaggtac tgttcgagat tactcattct cttttcaatt
     7081 gcttctttgt tatttttcca tgaaaaatat tctttcgcaa aatcctctgt aaaactttca
     7141 acgccagaag tattcactac cttctctttt ataaccactt tttcatgagt tgtatgttga
     7201 tcaatggcgg tgaaattttt ataaacgcca aaagcaagac tacaagacaa taaaagccaa
     7261 agggcaagca ccatttttcg atgcttaccc acggatacaa ctcgttcttt tttctttttt
     7321 gggctctttt ccttttgatt ccgttcaatt tttatcttca ttctttttcc tcttttctct
     7381 tatttgtatc ggccagctcc gataagatgc tgccgccaat agttttccgt tagattcgtc
     7441 caccccactg gatctcctgc atgaaacatg cgattctccc ctgcgtaaat gcccacatgg
     7501 gtcacataag taccggcatc ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca
     7561 tcttttagat caatatgagt catggcatca tattgatcct gagccacccg aggaagtttc
     7621 aggtcaactt tggcataaca ccattgggtc aagccactac aatcaaaccc cgtttctgga
     7681 cttgagccac caaacacata ggccgttcct tcatacttaa atgcctcatc aaaaatttct
     7741 tggactattt tgtcatcaaa ctgagttgta gttaggtatt gcttcactaa taaacagtaa
     7801 aacatattgc cataattata ccgccaccca ccattttcct taatcgcgat tgggttgggg
     7861 taatcgactt tttgaccacc tgatttttcc tttgaaaaag attcagctaa agcaaaacta
     7921 tgcttcttcc cgtttttccc gacatgatct agatacccgc caccatagtt ataggcctga
     7981 ataatactat cttgatcaca ccctagttct ttggaacgct taaccaaaga cgcaaaatag
     8041 agacagcctt gtttgatgga tgcctcggta cttaatgaat taggtggtaa tccggctgac
     8101 tcagaacttt gcataacgtc ttccattgtg ccaccagatt ccacttgaat aatcgccaaa
     8161 agaattggaa tatactctgt tatatccgat tctttggcat acttttccac cattgcacgg
     8221 tgtttcaata cttcttctga gacactagtt ccgccaggag aagtgccaga actaccattt
     8281 ccctcactgt cttcaccaaa caacaaaccc acacacatca aaatacccat aaaagagatg
     8341 gtcagaaaag ttaatgaaat aacacccact ttcttcaagt tcattttttc tcacctgacc
     8401 ttgattttgg acgatggttc attctttttt gtactttttg attggtctgc tttggcagag
     8461 tggacctctg tttcacttgt tggaaagaat ttttcttact tgctgattgg cttttccctt
     8521 ttgaaatgat ttcttgacta gttagttttc gtttgatttc gggattcaga tttgctctca
     8581 caatccgctt tggtgcttct tttttcagca tcatatcttt ctgaggttga gctgctttat
     8641 ctattggaat attcgccctt ctttttccct gacgatttgt tgcttcactc agtatcttgc
     8701 gtttttcgtc cattttcgca tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat
     8761 ccgctttttc ttggaacagt ccgcgcttga attcttctgg tgcttttttc gacttttcga
     8821 ttcctttatg gagattgtat ttcaaattcg ttggcgtatt ccttacctga tcctttactt
     8881 gtttagtctt atctttcacc aaagcttggg tatccaaaac tttgccgatt tttctcccag
     8941 ccaaattcat tcgggattgt ttcttattat ttgaaactgg attctcctta gttaagtcag
     9001 atagtatttc gtgatcgttc ggtaatcgtg aattctttcg tagagaagaa ccggatggct
     9061 taaatttgga ttttgattgt tcctttgcca acttatttcc gactgctgca ccaccaccaa
     9121 aagccaaagc tcgtcccaca tttcgattga gttgccgcaa ttttcgattc attaacattt
     9181 gcggttttcg catgacgcgt cttcccattg actggctatc attactctgg aggttgaaca
     9241 tactcataat gtcgcccaat ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag
     9301 caaccatgaa aaatggatag gttgccgaaa tattgaaaaa catcgtggag atgctaaatg
     9361 cagtcgtaat caccaaagta acacccgctc gcatcataat cgtattgaat aaacgaataa
     9421 tcgccttctt tcctaaactt tcataagtcg gcaacatgga caataagaaa ctaattggca
     9481 gaaacatcgc aaagataatg aagagaattt gcgagaacaa cataatccct gttagaagaa
     9541 aaacaaagaa ggaaatgcca atattgaaca ataccaaaaa gaccacggtc cctaaacggt
     9601 tcatggtctt ggtaatactg agattcgcat tgtcattgtc ttcaatctct gccttgaccg
     9661 cttcttcccg atctttgcct ttgttttcat ctggactcac agatagaatc ttgttgaccc
     9721 ggtcttcacc aatttcttct atgttggaat catcaaattg cagtagtaac caaggttgtt
     9781 ggacttgaat cgaaaacaaa ctatcccgaa tcaaatccac actatctttt ccttggcttt
     9841 ctgaattggg tacaacgatc tcggttccaa gggtaagggc cgcttcacta acatctgagc
     9901 taaagtcatt gatttttgtg atataggtag gcgcataagc aatgaacgaa cccgatagta
     9961 gaaaaatcat cacaaaatta atcacggctc gaactgcttt cgtggtttct cgttttaata
    10021 atccggtata cgccacatac acccccagag ctaaaatcat caataataga aacccaaaat
    10081 aaaatccgga ggtctggagt ccattttcag taataccagc caatgtttgg atattctttc
    10141 caatactttc agccgtatcc gaaatgaaat ctaatttgta agcctcttga accacataac
    10201 ccgtggcgtt cgacaaatac aaactcaccg tccaaatgaa attagtaatg gcataaagac
    10261 catacatcac gcttttccct aagccatcac cccaattcca aggcaaccaa tcccaagagc
    10321 tatccacaaa gaaatctagt tgatagttgt tcagtgaata tttggaatat agatttcctg
    10381 cttcaactgt atcatcaact agccctgtcg cctcggctac cgtaccaact aaactcaata
    10441 gaactatcag agtgagtatg ctaataaaaa cgattcccac aatccgaaaa agtttctttt
    10501 tcatcccctc actccattct ctcttgaaca ggtggacggg tatcaaaggc atggaagagt
    10561 tcttcaaaga ctggatgtac ctgaaccacg cctacccgac catacaaatc ctgcatcaag
    10621 cattggccat tttccaaatc tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag
    10681 ccaaagaatt ccaaggtttt cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc
    10741 aacccgatat tattcttcaa gcgttcatcc agtaaatcat cactattttg cgtgacaaag
    10801 taaaccccgg cattcatagc ccgcccagca cgaacgagct tattcgataa tgttttccct
    10861 tgcgctactt gaagaaaact ccacgcctca tctaagtcta caattttgaa gatttctcga
    10921 ttactatgga taaagtccaa ggcaaaagtg gaaatgacga ttagcatgga aacactaagc
    10981 aattccatgg tggtatattc ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct
    11041 acttgaatga tatttaactg cctatctaaa ctaatcgact gggtaatcga accatctgag
    11101 aaaagcaaat gggcaaaatc ataatcggta aaggactcga tgtgatccgc gatattttcc
    11161 gcaactggtg agccttcttt tcgaagctca ttaattactt gtaataatcc ccgttgattg
    11221 ctttgggtaa ccgtccgaat ggccttacgc aacactggaa acttctcccc atctcgacta
    11281 gaaattcctg ttaaaaaggt cagaatatca attgctagac tttcagaatc cttctttttc
    11341 tccataatca cataaggatc aagtaatcct tgattttttg gcttactagt taagttgaga
    11401 atattgattt catccgcaat ttctggcaag gtttctttcc agccaccccg ttcccctttt
    11461 ggatcgacaa tcacagcttg tccaccaaaa aggacggcgt aataaactaa gagattgtta
    11521 ctaaaggact ttccgccacc gagtgaaccg aggaatgccg ctgctagcgc attggtaact
    11581 gaatccttga ccccttgtga ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct
    11641 acgttataac caaagtaaat gccttccaac tcccctaaca tctgagtggc gccaaaacca
    11701 agtccggcga gaaaatcaga cgtaacatac tggatataat cattcatgta tcgcttacta
    11761 gaaggtaaga attcttcatg tagccccatc atatcgccaa aaggccggac tagttttaca
    11821 ttggtatcat cataaaaatc tagcacttca tcacaccggc gctttagttc atctactgat
    11881 tcggcactta ctcgaaccac atagctcagt ttgtacatgg attctttgga ctgatccaag
    11941 gtagtttcca attcatctac ggaatccaat gcatctaaga cattggaatt tgtttcattg
    12001 tcagattgat aggcatggtt gtccaagtct tttaattcct ttttcttatt gcgaacggta
    12061 gcaagtgcct ttttgttcgt cacaatctcc acattcatac tagtgtcaat tgggaatgta
    12121 aattgttgtt gctggtaata gaaaagttct gaggagggaa actccatttc ccccacaatt
    12181 gtattaattg ttaagtacgc tacatatgat tcatgattct catgttccat gcataagtac
    12241 cggggctttt cttcaatcaa acaacgactg ggacgtaata agtcgtaacg tttcaccaaa
    12301 gtttccgact tcagcttttt ctttggtagc tgaaattcat attcttcaaa tggtgtaccc
    12361 ttttcgccat agatatgttc aatcaaatac atcagatcac tcggtgtgac tcgtctgact
    12421 ttaaatcttc gagccaattt actttccatt agtttttcaa gcttggtata tcggcgaatt
    12481 tcttcattgg ataaggaaac aaaatcgccc atcaaatgat gattcactcc atagacaaat
    12541 tcttgaaagc cggagaagaa agatttcttg agattcttta gattgacctc ttcatctgtc
    12601 gcaatcagtt tgaagccaat aaaaaagcga taatcaatct gactatcgcc aatcatggat
    12661 actaatgcct ctgtctgtaa atcaattcgt tgtttcgcta cttctgttaa tcgcccggta
    12721 atttcctttt ttgatcgttc ttgcgtggca cgaatactac tttctgtggc aatttgtaag
    12781 gcatgaatct tgccttcacg attttgcgca atcaattgac gaaagttatc gtggacttga
    12841 tatttctgtt caggtgacaa aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa
    12901 cattctccct cttgattaaa gacaagatta ttttcaatat acttaatcgg gtacatagtt
    12961 cacactcctt accagagtaa tgggttcgtc ccatcgtttc ttctgttctt tgactttctt
    13021 tcctgcgaaa gtgaccttaa atcgtaagtg atagctaata aaagatcgta aaaatcccaa
    13081 tggtttctta ccgtcaaacg ttttttgcga catgaaccaa gtaactgcca ccggtatacc
    13141 taagtatttt aaaaaggccc cctctatcat agatagtgga ggcacatggg caaatacgat
    13201 gacaaacagc aaggataaaa caaaccaagc catctggtta aaagtcacag gaaaaggcag
    13261 ttgaaagtca ttaattgcat aaatgacctt ttcaactgcc caaatactgg tgtagctttt
    13321 tattttcttc atcaaatcat tcctttaaaa agaaggatag tttagaaatc aactaccctt
    13381 caataatttt gttaatatgg atactcaaaa acacctcgac tcgttaccaa ataattgcct
    13441 ccaatctcca agtctctacc acacgcatca aaatcaatat acgtcttcaa gtgttctggt
    13501 acttctccaa aatcaccagc ttctacaatc aagaaatatg ctacatcact catatcatcg
    13561 caatctggat aatagataat atcatccaca tgctctacca tctcctcaaa agaattgaag
    13621 aaagcatgtt gaatctcaga agcaacttca ccaactggcg ttccttcaag ttcctccgct
    13681 aagccacaca accgattaat ttcctcaatt ggcgtatact catcaatctc aaagggaagt
    13741 tcataatcat gaattgcata ttcctcgtat tcgtcattta acccaattcg ttctttcact
    13801 tcatcaaaat cgacaggtgg ggtaaaccac gcccccacca attcaccttc gttatatttc
    13861 ccgaggttcg caatataaac acgcatctgt tccatcgtgc ttccccctta cgcaccgaca
    13921 attcggttaa acaaactgag taagacatcc ttcaccccag aagcattaaa gaccaatccg
    13981 accgcaatca aagcaatcac caaaaagcca atcagtttgg aaaactcccg cttaaatcct
    14041 aaaaacaaac caatgaccac aattgccagt aagactaagg attgggcatt ggttaaaaac
    14101 cattgatata tggataggta agttcctgat attataaata tctcaaactt ttccccccat
    14161 tcacacggag cgtgcgactt tcaccgcact ccgcgttcca tcgtgtttat attgaaaagt
    14221 atttatgtag tagacctata tcaagtttaa ttcttgattg ccagctaata ctctatattt
    14281 atttagtctt atgagctgga ctttatttag cttcaataga gctatatatt tttgtattgt
    14341 ttcttctgtt ttagcgtgta ttagacggtg taccgcctct tttaccaaaa taagattttt
    14401 ataatcgtca tttccaccag ctccagttgg tacaatatga tgacaatgta tttcattagc
    14461 ttgttgaaat tcttccagag ttatagcaca ttttccatgt tgagcagaaa atagcgaaat
    14521 tcgattgtct gcgtattcta ttgtgcgatt ttccaaagat aatttcatta actggtacat
    14581 cagtaattta tcaacactta gattggtgtg gataaatttt cttccctcta gagtgtagca
    14641 attcgctaaa tacacctttg ccattggttt tctgtgttgg atatatccaa cagggaaaat
    14701 aggttcgccc tcgccagcaa cataccgaag cattttagat ttaccatatc ttgttgcctc
    14761 ctttgtcgta agttgtctgc ctgtttttct caatctgccc cttttgtttg tacctaatct
    14821 gttagtgaag atagccatta cagctctatt cagtttcata caatccagat ttacattcgt
    14881 tgcaatctga taataatttt gcatacctaa taccattgaa ttataaagtc ttgtttcttc
    14941 taattccgtt ttcccttttg agggagaagc aattttcttt gcctgtgata ccagcttgtc
    15001 tttttggttt ttgaattgtt tatcacttat atgtgattta acaactaact tctttcttct
    15061 cggtatcatt ttaatcttga aaccaagaaa gtcggagtat ctttttctaa catttacaat
    15121 tcttgttttt tcttgtgata cttccaattt taatcgttgt tcaatccatt gagtgattgc
    15181 tattttggct ttttcagcac tttccttgta gcggcagaaa actctaaaat catctgcata
    15241 acgaaccata tacatttcct ttaactttgt tttcttcatt tctttgtaag cgttgctttt
    15301 acatgggcta ccgcttttat tgatatgaac cacatagttt tttgtaacag gattttcctg
    15361 ccattggctt tctacccaat ggtctaactc attaagaaca atgtttgcta gtagtgggga
    15421 tattattccc ccttgcggag ttcctttctc tggatatgtt attgtaccgt tagtcatttt
    15481 gattggagct tttaatatcc tttttaaaat gaatatcaag tgcttgtccc gtattcccat
    15541 agcccatatc tgttttatca gcttggaatg gttcacgttg tcaaagaaac ccttaatatc
    15601 aaattcaatc acataatgta gctggctgat ttgtaggcgg ttataggttg cttttattgc
    15661 attttcaact gacctattag gtctaaaacc atagctgttt tcactaaatt tagcttcgca
    15721 gattggttct aatacttgtt ttatacattg ttgtataagc ctgtcccaca tacaaggaat
    15781 acctagaggt ctggttttac catttggttt tggtatttct tttcgcctca ctggcttagg
    15841 tcgataccca tgtggactgc ctgcaacaat gtatcttact tttttgacaa ctgtttctgc
    15901 tggcaatttg ccaatatctt taattattgt accgtctgtt cctgctgttt tactgcctgc
    15961 atttgctttg atatttcgat atgccagtaa tatattttct ctcgatagga taatatccat
    16021 aagatgagtg aaaatatctc cattcttact tttggcatat aaatcatcaa aggtgttttg
    16081 catttcgtaa tattcagcgt gtcttaaatc ttctacacac aatgtttcat ttttattttt
    16141 aggcataagg cttcactcct ttacttgaaa ttgagtgagc ttttttatct tactcgtaat
    16201 ccttattgaa acaacataat acttttctaa tatatacacg acttgagggc attcctccat
    16261 tctcattaca agaatttctt aggttcgccc tctgcttttc agcattagtt caactgctta
    16321 ttgttcttcg tcagtttatt ctcttgatga gaacccaata cctttcctcg ttccgataat
    16381 cttatctata catatatatc cttaggtcac tagctataag cctgctggct tgataccgcc
    16441 tgtaacgtta tatggcattt cataacctat attcttactt tgccacacca gctacgattt
    16501 ctcgtacctt tgcatttcta caaagttcaa atttagaccc gtacattcgc tgtttcgtca
    16561 gttccttata gaacattcta accatagata ttttatagac ccccgacctg ttggatacgc
    16621 tacccacctc tggatagttt tcgatagaag atatatgttc atcaactatt acggtatctt
    16681 tcagccgtct ttaccgagct tcaaacattc tgagtttcct catacaatgc ttgtcggagg
    16741 ctcagggcag gcgtttcaga gcgttacttc atcattccac ctgtcagatt atcagttctc
    16801 ctaataacca aactttttcg ttcttttatt agtgccttag cttttcacta aggaacgagt
    16861 cgcacataaa ttttgtccga aattcatctt gtcactttcc tttcttttcc attgctcgat
    16921 ccgctgctgc acttgcattc atcaagcaca tcacagtaac accaagcata ctgccaccaa
    16981 ataccaaaaa aatatctttt acaattccca tgacgattcc tcttttcata taatcaaatt
    17041 ttcaattgcc atactttgtt gctccaaaat ctttttatgt cggtctgata agcgtgcatt
    17101 ctgtaccata tcctggatat aggttgtccg attcaatcga tccaactcct tagccatttt
    17161 ccacgtaggg gcgacttgtc ggcctagcca attcaaggtt cgttcaatgg tatacggttt
    17221 tggctcggaa gttaattgaa tctctttccg attccgtcca ataaaacgcc cccattgttc
    17281 attcaagggc cagttggttc gccttttacc ttctacttta tccgcaaatc gcaagtaacg
    17341 attgataatg gaaaacgtgg tgctctcaat gctacgacct tttaacaaat cttgaatcgc
    17401 atgaaaagca cggtcatttt tcagtcgaat ctcaaaacga tttttcgtct cagtttggtc
    17461 taatggaatc cctaacttga tatattgttc atagtctttt tcatacaaac agaaataaac
    17521 ctcactcttg agactgccga tatataaggt attgcccatg ccgtcctttt catcagcctt
    17581 taaaagttcc ccggaacgat aacttttaaa ggtacgaaac aaggagatac attcttcctt
    17641 ctgacatttt ttcgttagct caggtatatc caatagcccc actcgatcat tgattgccaa
    17701 gtccaagcgt ttcatcacgc caccggcctt tagacaatcc tcgaaaaagt cgtaccagct
    17761 acgcttttga gcgagtaaaa aagtttcaaa ttgtcggcaa cctcgcccct ttaattctaa
    17821 aagcacacct ttatcttctt catgagacaa catcaccaca atatcaccca tgacatactg
    17881 ttcttgatag gagtaaaagg cataatcttc atggagcata tactcaaatt tcaaatggag
    17941 aatttcttga ataatttttc gtgcatccgt tgtgggaaag cgaatcctta catagtcaaa
    18001 cagtaacgtc aaaggcaaat cgggattaaa gctttccagt tggtcaaaaa ttttcctcat
    18061 ggtttttata gatgcggtta cgccaccatt ttctatctga tttaagtacg gccgactaag
    18121 tccacaagcc gtagccaatt tattttgtga aacgccgtat tcgttgcgtc ggtcttttaa
    18181 tagccgataa tctaaattcc gttgtgccaa gttctcacct ccaaattcaa ggtgttaact
    18241 ttttcaaatg agttaacacc tatacaaatt acttactatc tttgatacaa caagcattct
    18301 taatggttgc ttacttattt tttgtgtttt tgtacccccc tgttagatac gggggtgaga
    18361 catcatggtc ggctaacgcc gtccatgaac aggcacagca ccacggagga tttctgcttc
    18421 gcgaatcctc cgcggtcagc gcctaacagt cagttgtgtt ggtttaggtt actcatctgt
    18481 aataccattt acttccaact ctgctttcat ttctaacaag tattgacgaa tatctggttc
    18541 ttttgcttgg tccgcaatcc gctcaaattc tgcaattact tgatttattt caattcgagc
    18601 atccgtctcc tcattcatta aatatggatc gccagcgact ttgacaccat tcttgcgaat
    18661 atcaaatttt aaccgtcgca attgttcttc taattctggg tctgactgaa tctttccaat
    18721 cacccaataa atttctccaa tcaactcatc ataaaccatg tacatccaac tcttttaatc
    18781 ttctggcaat atcaaaaatg accggaactg ttacagagtt tccggcttgc ttatacaact
    18841 gactatctga attgacttga gaatctcgat caaaagccca atcgggaaag ccctgaagtc
    18901 gccaacactc tctaggtgtt agcttccgaa tttgatactg atccgtgacg atcgcttgtt
    18961 ggtctcctgt tagtaaggta tgaacactct gttttcccag acgacctcgg cgacttgtgg
    19021 aattaggaaa agccagattc acactgtctt ctggcttcac ctcttgatat tctgtttcat
    19081 tttttcgaat catgattttt gtaccacctt gtttcgttga caaggtgggg gcggttccag
    19141 taacttcata aactcgatta ccttgttcaa actttcccgg taatagtcct gctacagaga
    19201 ttccatccct tgctggcagt gcgattttga ttccttcatc acctgttaag gttggcgcta
    19261 cgccatctga accatagact tgtccaccca ttcctcgatt ggaaggattg atatttacca
    19321 aatttctgat gttttgatca gatgttgaag acggtccaat gataggaaat acttgttcgg
    19381 tacatgcgtc tcgagaatgt ccgataagga atatccgctc ccggctttgg ggaatgtagg
    19441 cctttgagtt aagcacctac cactccacgt cataccccaa tccatccagc gttcggagga
    19501 tcgtctcgaa cgtagcccct ccttcgtgat tgagcaatcc tcggacgttc tcaaggaata
    19561 aaagcttagg tcggagaaga gtggtgaacc gagcgatttc aaagaataaa gtacctcgag
    19621 tatcggaaaa tctttgccgc tttcccgcaa ttgaaaaagc ttggcacgga aatccgccgc
    19681 aaaggatgtc cactggtccg agggattgaa taaactcatc tgatacactt gtgatgtcat
    19741 gcatttccac ctccttactt gtatcatgaa ttgctttata actacgtcta gcaaatttat
    19801 caatttcaca aaaaccgata caatgatggc tcgcctgttc catacctaaa cgaaagccac
    19861 caataccggc gaataaatct aaaaatctca tacattacct cattcattgc aaagaaaaaa
    19921 gccaagaaca ttttgctctt ggctcataca ttattaattt gtcctactat tttttattta
    19981 atccaactaa tttccgttgt ttttcattgg cctgatcggc tacatattta atcaccgttt
    20041 tgaactcttt tgcagtagca ttttccgata aatccgcaat gctcttattg tatttcttta
    20101 caattttcgt tacttgttct ttctcagtca tgtaattgaa cctccaaatc tttcctacta
    20161 taccctagtg gtaaaaataa ttctagatta tccgtagcat aatgaacttc ggaaatatta
    20221 ttcgtataat cttttattat gatatgaagc aatacatctg gatgtttact ctttactttt
    20281 gcaatgttct ctcttgattt gaaaaaagcg ttaataaatg cctttttcgg cacatgacgt
    20341 ccctctgcaa tttcacgttc ttgtgtaaat ttccaagcga ttttagggtc ttgataaaca
    20401 tagaaaatcg tcactcgaaa atgttttttc aacgcccgcg agatattttt ttcagcactt
    20461 aatattgcaa aagttgcatc cagaataaaa gaatagccat gatctaagac atatctaaat
    20521 gcctgctcta ccaaccaaga agatgctttt tgaaagagac tagagttttt tccattataa
    20581 tcaggaaact gggtgcgaaa gtaatccgca tcaataatca catggttatc ataattctca
    20641 gctaatccaa tggcaacctc tgattttcca gccccaggac tccctgccat aaaaactgcg
    20701 tctcgtccaa cttcggggga tttccctttg ataatataag aaataaactt atccttattt
    20761 gcttttgcat actctaaata agaatcatta ctttccatat taattatccc ctttcttcct
    20821 actatataaa agtattatac gttacttgaa gtataaagtc agtattgaag atcactcaat
    20881 cattcatctt ttctaattta ctaattgaat ctaagaaatc ataccctttt ggtacaagtg
    20941 gcgtataaaa ctcactaatc acactgccac ctgtatccac ataacctcga cctttgatgt
    21001 gcttcataaa gaagtttttt tcgacctccc caaacatcat ggaatatccg agttcactca
    21061 tacgacccaa agccacgcga aagttgaatt gatcgcggat tccgtctcct aaatatttcg
    21121 catccggcct ttggcaagcc aaaatcagaa agaaaccaga ttgacgaccc aacatcacga
    21181 tttgtttgag cttattcaaa atcaccgcac tttctttcgt cgttaacatc tccatataag
    21241 ccacgtattc atcaaagatt aaaaagttgg gtggaagtcc aagataggca taattctctc
    21301 ctgttttgta gttagacatt tctttcattg ccttgctacg agccatcata cgttcataga
    21361 aatcttccac acaagcagaa atttcttcct tttgtgaata aacatgaggc atcaccgtac
    21421 ctaaatcagc taaatctgca tttttgggat cgaggataaa caattctgca tctgacttca
    21481 atagtgcttc gataatggtg aggaggaaat aggtcttccc tccacccgtg ccaccagcga
    21541 ttaacatatg gggtaaagaa tcataagccc atacttgatt tttcatcaat cgcagagtcc
    21601 cattttctgc cactacttcg tctatcccaa ttcgattggc gatcatatca tacagtaaag
    21661 tatactccac ataggagtct ttgagttcct tttctactaa ctcacaatac agtccgcttt
    21721 ctaacttctt ttccaatttt aaaagctgat cttggtattt cccaagtgaa atttgaacgc
    21781 gaatagacag taatccatct ttcattcgat aacagatttt ggggaagtaa ctgatagtct
    21841 ctctggttcg gctactattc aaatctttga aaaagccttc actttgagtt tgtttcactt
    21901 cataccaatg gttctctaga atcatccgag ctagcttttg tcgatgccac aagcttcgcc
    21961 aatgatcaag aaaaaagtga atataaagca atatcatagc tcctgaaaaa gcgagacttc
    22021 caagcagaca tactaaatct tttatagacc aaacgatttc aatagtagaa agtgatagct
    22081 tagaaatatc aaattgtcta aactcttgaa aataaaagac gctcatccaa ccgatgaaca
    22141 gagcgagtag ccagccaaga acaaagtgat aaaccaagtg ctgatctcct gcgcgtatgc
    22201 gatgtccttt gtacattttg atggtttcca cctccaatcg aaaaagccca ataggatttc
    22261 attaggcaat ttcaaaaagt tatgctagtc atttactgct acgaggaggg gagttgtttc
    22321 gtttcaacca attgatttaa aaagccttca atagcatccg gcatctccac accaatttct
    22381 tccaaaatgg attctgcctg ccgacataag ttttcattta cttcgacaac tataaagaaa
    22441 gactcttctt ctcctaacca gtctagtaca tcagagtctt cttcactgtc gctattttct
    22501 ccaagtaaac cttgatgtat aatcagaaac attttttcat cagaggttaa tgctttaacg
    22561 agttgttcct cagtcagctc gccttttcca tattgatcga gacaatctaa aatatgttca
    22621 acattaattt ccataatctt cacctatttc tttctttcat tttggttcgt aggatttcct
    22681 tgattcggct gccgcttcaa gactaaatca tccgccttaa tgaaccaatt aacggtagtt
    22741 ccttctcgaa aaacataatt ggcaacggta tccacgattg gattgaccaa ctctacctct
    22801 gcatcataag caaactcgcg taagggtaca ttagctggga tactaacttg aatcattcgc
    22861 ccttgtgcag aggattttaa attataggtt cgttctttta cctcattacc aattgttcca
    22921 tcctcatttt gcttccgtac ttctcggcgt aatgcggaaa aacgcaactt cccaaatgtt
    22981 aaacctgaat ctacgacaat accttctgtt aatctcatct tcttttcctc ctatgctttc
    23041 actaaatcat caacatacaa cacgtaatca gtaaacccac gattctcaat ctcatagcct
    23101 ttggcaacca aatggggatt cacggctttt agcggttggt cttgatcaaa atccttttca
    23161 ccggcttccg cagctactac cacctcaata tcatcggccc gttgcttact ggaatacaaa
    23221 tgataactgc gaccaatcac cgtagtattc cgtccatatc cttctgttat aatttctcct
    23281 tcaccggcat aacttaactt tccaaaggtt tcagccatat ctggcacgac aaatttcaat
    23341 tccatcttct tttcctcttt tcattgttag ttttggatac aaaaatagcg cgactacttt
    23401 tttaggaggc ggtcgcgcac gttggttctt aggatttact tgtatcatct catcacctta
    23461 gttccggcga ttccagaaat aatagcccgc cgtcgcaaaa atcaaagtaa aaccaatgaa
    23521 cagtaaaaca ttggaatttt tctccccggt ttgagggaag gtcttcactg tgggactaga
    23581 ttcttgagag ccacttggaa cagttggtgt ttccggttgt ttcggcgtac ttagtatggt
    23641 tggaacttct tttggtgtta aggtttgaga tttttctttc aaatcttcat tgtgttttcc
    23701 attgatattc ccatctttat cgtaattgat ttccgcaaaa gtaaacttgg ttccttctgg
    23761 atattttccg gtatctactt ttttcgcaag cacagttttg gtgaattctt tatcatttac
    23821 ttcataatca atcttgccag atttccaaat ttctttgttt gtaccatccg gtaataaagc
    23881 atacagaatt gtttcgaaag cttcttttga gccatccagt acatcatgag taatcgacac
    23941 atcatcaaac atatccacca catcaccatg agtaaaggtc tgagaaccgt tttctaggtg
    24001 ggcctttgtt tggatagaaa catggtgttc cactgtacaa ctgacagttt gggcttggtt
    24061 attcaagcta acatccttcg ctactggctt atcgcctgct tcaaaagctt ccttattttc
    24121 atacacatag ttgaataaga caatagtttt atttaaaact tgttcggcgg ttaatttatg
    24181 ctgaattttc caagttcctg ttttctcttt gttcgccaac gttgcgcttg tttctgcaat
    24241 caccactggt ttggcttttt catctttttc ttgggcagct tttgtcgcat ccacatcaat
    24301 ggcttgcgct accacatacc agtcttcttt gatttcatgt agattgtaac tcaatttatc
    24361 aactagctcg gtagaatcca gagaagttaa ttctttgttt ccgtctttcc aagtcgcaag
    24421 agaagttaaa ctatcttctt cctcaggcaa atcatagagc attaaacggt tcaaactaac
    24481 agaaaatgct ttgttcgtca gcttctcgta aggaacggtc accgttttaa ttggttgttt
    24541 ttggtcttgt tcggtaatgg taaagacata ctcactcttc acaaagtctt ctttgttttc
    24601 cttaaaagta gaacggattt ctaatggcgt aatcttttga aaaccttctg gggcttctac
    24661 ttcttcaagt aaataatccc cataaggaag attttcaaac ttgccataac catcaaaccc
    24721 taattgctcg ttataagctg tggtcgcttc atcttcggca cctgtgattt cattggtccc
    24781 ttccaatggc gacactttaa aggtcagatc attgaagcca gtttcggtag tgcccgcagc
    24841 tgatccagca aatttaaaga aatcaaagcc aaagcgaatc acttgttctt ttgccgtaac
    24901 atttcgggta atcacggcat ttttttcgct atcatccacc tttttgatgg atacaggata
    24961 ctttgtttca tcaaggctat atccttcggg cgcttttgtt tcttgccaat aatactcgtt
    25021 gatagctaaa tgtttaacgg caacttggtt cttttcgtct aaagtcaaag tgaccgtttc
    25081 atcagaacct ttcgttccct tcgctaactc tggtttaaaa gcctcactcc atttaacagc
    25141 ttttccatcc ttagcagtaa acaaggtgta ttcagcaccc tcaaagactg ccttaccttg
    25201 tgctttatcc ccagtatctt tgtcttcttt cgtcaaagtg gtttcgccag tgacttcttg
    25261 attttgccct tttacgttac tggtaacagg ggctacggtt tgattggcat acttcaattc
    25321 gacttttaca ggtttgaacg tattcacgaa cccatgacta gctttagttt cggtcacata
    25381 ataggttccc aattccaacg cattagcaat ctcttttggt gtttccgcat gaccgttttc
    25441 atccgtggtc atttcttgaa cgatttcacc ggttggactg tctttgcgaa tggcaaacgt
    25501 attaccagct aaggaataat tgtcattcca aagatcactc cctgtttcaa ctccgctttt
    25561 atccagaatg atttgccctt tttcccgtgc gttttttgaa gtcacgtaaa tggtttcgcc
    25621 tgctttaatg gtggtagtca tgggggtggt atcaatcgta taaggcgccg gtactgattt
    25681 ttcggtaatg gttacttttg taccatgagg aatctcatcc aatgtagcaa tgccttcttt
    25741 gtcagtagtc acgtcttttg caggtaaagt ttttccaaag tctaaatgga aaaccgttcc
    25801 tggtacaaca tcacccgatt ctttatcgac ttttttgatc tttaaagaac cctccgtttc
    25861 cacatcaatt ttgattgtgt aggtattagg tttatcaatc gccccggcca ttaaagtctg
    25921 ttggccagcc tttttatagg cgacaggtgt tccggtacca gcagatttct taagcgtcag
    25981 cacaccactt tcttttgaat tggcatttgg ggtgattatc aactgattac cattcacacg
    26041 ataatcaatg tttgcggcat tttccacaac ctcatcgaac tctgacaaat tcagattatt
    26101 cgtatccgtc acagtagtcg attgccctaa tactgtttta gccgtactat tatggaaggt
    26161 tggtttcttc tgataatcgt caatggcttg atttattttt gcctcaatgg ctgcgatatt
    26221 tacggcacta ccatctgatc gttttattga gtgaagggta tacccattaa cttcttgcca
    26281 gatcatcttc tgtgccacaa tatgagtatc tgcatctgta cctacttttt tccataaaac
    26341 ggaaactaat ttggctttat cggacatatc tggtaacggg tttttctcat agccaggagt
    26401 gaactcattg taaatcggga ctcctggttc aatacagaaa atatcttgtt taaagccgtt
    26461 gtacactgca taatgcggaa cagtgacttc tgacattgga cgaccatcac taaaagttcc
    26521 ttcaacagca tattgatgtg ctaaatctag ttctacggtt accgtttgtg gatgggtaat
    26581 ttcatcagca aaagtaatcg taggagcaat tgctccaaca attgcttgaa cagccaaact
    26641 caaaatagtt aatacacata atttcttttt ttgtttttta tacatgaaat gttctccttt
    26701 caaattaagc acaaaaaaat aagatgctcg cttgaacatc tttgtttata aagtcttttt
    26761 aaaattttta ttgactgaat agaatggatt tgagaatgaa tgagcctaaa aatttaatct
    26821 aaaatacgaa atatactgcc tttaaattag aacacatttt aga
//
LOCUS       DASJTC010000033        26602 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.033, whole genome shotgun sequence.
ACCESSION   DASJTC010000033 DASJTC010000000
VERSION     DASJTC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.033"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(19..729)
                     /locus_tag="U4B19_001247"
     CDS             complement(19..729)
                     /locus_tag="U4B19_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEN2076996.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="U4B19_001248"
     CDS             complement(726..2198)
                     /locus_tag="U4B19_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2076997.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="U4B19_001249"
     CDS             2336..2809
                     /locus_tag="U4B19_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2076998.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="U4B19_001250"
     CDS             complement(2962..3513)
                     /locus_tag="U4B19_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2076999.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="U4B19_001251"
     CDS             3618..4148
                     /locus_tag="U4B19_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEN2077000.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="U4B19_001252"
     CDS             complement(4631..5074)
                     /locus_tag="U4B19_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2077001.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="U4B19_001253"
     CDS             complement(5090..6250)
                     /locus_tag="U4B19_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2077002.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="U4B19_001254"
     CDS             6757..8457
                     /locus_tag="U4B19_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077003.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="U4B19_001255"
     CDS             complement(8559..9392)
                     /locus_tag="U4B19_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEN2077004.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="U4B19_001256"
     CDS             complement(9696..11861)
                     /locus_tag="U4B19_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2077005.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="U4B19_001257"
     CDS             12121..14415
                     /locus_tag="U4B19_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN2077006.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="U4B19_001258"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="U4B19_001258"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN2077007.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="U4B19_001259"
     CDS             15066..16073
                     /locus_tag="U4B19_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2077008.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="U4B19_001260"
     CDS             16095..17114
                     /locus_tag="U4B19_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2077009.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="U4B19_001261"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="U4B19_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEN2077010.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /locus_tag="U4B19_001262"
     CDS             complement(18871..20292)
                     /locus_tag="U4B19_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2077011.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="U4B19_001263"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="U4B19_001263"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN2077012.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /locus_tag="U4B19_001264"
     CDS             complement(22703..23959)
                     /locus_tag="U4B19_001264"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEN2077013.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24071..24276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24539..25840
                     /locus_tag="U4B19_001265"
     CDS             24539..25840
                     /locus_tag="U4B19_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEN2077014.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="U4B19_001266"
     CDS             complement(25909..26508)
                     /locus_tag="U4B19_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2077015.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         8075 a         5477 c         4765 g         8285 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
    26581 tatctattga gaaagtatca ag
//
LOCUS       DASJTC010000034        24929 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.034, whole genome shotgun sequence.
ACCESSION   DASJTC010000034 DASJTC010000000
VERSION     DASJTC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.034"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(86..697)
                     /locus_tag="U4B19_001267"
     CDS             complement(86..697)
                     /locus_tag="U4B19_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2077016.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(820..945)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1027..1743)
                     /locus_tag="U4B19_001268"
     CDS             complement(1027..1743)
                     /locus_tag="U4B19_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN2077017.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(1749..2318)
                     /gene="scpB"
                     /locus_tag="U4B19_001269"
     CDS             complement(1749..2318)
                     /gene="scpB"
                     /locus_tag="U4B19_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEN2077018.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(2302..3099)
                     /locus_tag="U4B19_001270"
     CDS             complement(2302..3099)
                     /locus_tag="U4B19_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEN2077019.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(3069..3440)
                     /locus_tag="U4B19_001271"
     CDS             complement(3069..3440)
                     /locus_tag="U4B19_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN2077020.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(3459..4346)
                     /gene="xerD"
                     /locus_tag="U4B19_001272"
     CDS             complement(3459..4346)
                     /gene="xerD"
                     /locus_tag="U4B19_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEN2077021.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(4442..4915)
                     /locus_tag="U4B19_001273"
     CDS             complement(4442..4915)
                     /locus_tag="U4B19_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2077022.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(5020..5883)
                     /locus_tag="U4B19_001274"
     CDS             complement(5020..5883)
                     /locus_tag="U4B19_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2077023.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(6079..8178)
                     /locus_tag="U4B19_001275"
     CDS             complement(6079..8178)
                     /locus_tag="U4B19_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEN2077024.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(8398..9507)
                     /gene="rpoD"
                     /locus_tag="U4B19_001276"
     CDS             complement(8398..9507)
                     /gene="rpoD"
                     /locus_tag="U4B19_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEN2077025.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(9531..11372)
                     /locus_tag="U4B19_001277"
     CDS             complement(9531..11372)
                     /locus_tag="U4B19_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEN2077026.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(11554..13869)
                     /locus_tag="U4B19_001278"
     CDS             complement(11554..13869)
                     /locus_tag="U4B19_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2077027.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(14054..14914)
                     /locus_tag="U4B19_001279"
     CDS             complement(14054..14914)
                     /locus_tag="U4B19_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEN2077028.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(14973..15494)
                     /locus_tag="U4B19_001280"
     CDS             complement(14973..15494)
                     /locus_tag="U4B19_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEN2077029.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            15772..16533
                     /locus_tag="U4B19_001281"
     CDS             15772..16533
                     /locus_tag="U4B19_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2077030.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            16559..19150
                     /gene="mprF"
                     /locus_tag="U4B19_001282"
     CDS             16559..19150
                     /gene="mprF"
                     /locus_tag="U4B19_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEN2077031.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19338..20525)
                     /gene="tuf"
                     /locus_tag="U4B19_001283"
     CDS             complement(19338..20525)
                     /gene="tuf"
                     /locus_tag="U4B19_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEN2077032.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            20893..21249
                     /locus_tag="U4B19_001284"
     CDS             20893..21249
                     /locus_tag="U4B19_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2077033.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(21490..22494)
                     /locus_tag="U4B19_001285"
     CDS             complement(21490..22494)
                     /locus_tag="U4B19_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEN2077034.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            22660..23511
                     /locus_tag="U4B19_001286"
     CDS             22660..23511
                     /locus_tag="U4B19_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2077035.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            23528..23773
                     /locus_tag="U4B19_001287"
     CDS             23528..23773
                     /locus_tag="U4B19_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077036.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            23960..24664
                     /locus_tag="U4B19_001288"
     CDS             23960..24664
                     /locus_tag="U4B19_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077037.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         7013 a         5169 c         4355 g         8392 t
ORIGIN      
        1 aaacaaataa aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca
       61 agaaatttct tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat
      121 gttgttttcg acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca
      181 cgatgaatcc tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag
      241 acatgcccag catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa
      301 cgaaataatt tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta
      361 aaaatttgtt tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg
      421 taaagatcgt tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac
      481 ctgtagtcag cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac
      541 cgaataagaa catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa
      601 aagccggcat gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg
      661 ctactgcaac cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc
      721 ctcttcagcg aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa
      781 atagagttag agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac
      841 tatactgtcg gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga
      901 ctataaccac cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg
      961 tttacatatt cattatacat atctattcaa aaaacgcaac atctaacact tataaaaagt
     1021 aagtgttcat tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta
     1081 atccccagga cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg
     1141 cacaggaaga ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat
     1201 cgtcaattcc acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc
     1261 tggagatgtt tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt
     1321 tgtagcgatc ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt
     1381 caggcgctga gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc
     1441 cagtcgacct acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt
     1501 tcttcctttg tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta
     1561 accgtattct tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac
     1621 ttttatccct aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa
     1681 ttcttctgct tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc
     1741 cattacaatt attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct
     1801 tcaagtgaaa tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa
     1861 taatctaaga agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta
     1921 cctttttctt caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact
     1981 cctcgaatct gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag
     2041 gtttcgacgg cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag
     2101 ggagccagct cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa
     2161 gctgagtgcg agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct
     2221 gcttcgctga gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca
     2281 ataaaaagaa gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg
     2341 ataaagcata attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat
     2401 caattctagc aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa
     2461 agaatcgaaa gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg
     2521 ttcttcgatc gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt
     2581 acgttctaag attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt
     2641 caacggcttt tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag
     2701 acttcgttcc tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc
     2761 taaaagctga gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt
     2821 gacgaattct tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat
     2881 cactaagtat tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat
     2941 atattgctcc gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat
     3001 cagatgaagc agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt
     3061 caactcattc actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg
     3121 atttcagttg cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga
     3181 aagcccatat tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga
     3241 tcgttcgttg cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta
     3301 aagaaaaaaa gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa
     3361 gttttacctg tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct
     3421 ttccggtaat gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc
     3481 ttataaacat ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata
     3541 tccgcgtgac ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt
     3601 gtggcaaagc tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt
     3661 actaactgct tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg
     3721 acaaatacat gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag
     3781 cgttccagcc attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt
     3841 ccaactgtct gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat
     3901 tcactgacgc gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct
     3961 aacgtttttg tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta
     4021 cttggtaatt tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg
     4081 atacgctctt gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca
     4141 gttgctgtcg cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga
     4201 tcgacatcct gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa
     4261 tcacgttcat agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg
     4321 agataatcgt tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga
     4381 taccgcttca ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca
     4441 ctcagacgga ctggtttctt ttttcttttt catagcattc ctgacagatt ccatggaacg
     4501 ttagccgatg atcttttact aaaaaatgat aacgttcttc tactacttgc tccacttcaa
     4561 gtaaaagatc ctcttctact tcctcgatcg tgccgcagtt caagcatagt aaatgatgat
     4621 ggaaatgttt ggcgccttct tttctcaaat cataacgggc taatccatca ttaaaactta
     4681 ctttatcgac aattttcaat tctgtcaaga tttcaagggt tcgataaaca gtagcaagtc
     4741 caatttccgg actctttttt ttcactaaaa agaagatttc ttctgcagat aaatgctctt
     4801 tctcattttc caaaagcaca agcagagtag cttctctttg cggtgtcagc ttaaaccccg
     4861 cttcatgtag ctgctgtttt gtttttttca tcgccgaaat agcattcatg accatttact
     4921 caaccccaac aatttataat aattacaaat aaaaaaagat ataacactat gttaattaaa
     4981 attattataa gctagttaag aaaaacatgc aagggattct caattagatt ttttgactag
     5041 ttttgtcaca ccgttttctt gtgtaatcaa tccttgtttc aaaagattac ctaatgcgcg
     5101 cttaaatgct cctttgctga taccaaaagc tttcatgatt tcttctggat ccgatttatc
     5161 tgtaaagttg atttgctgat ctgcagaacg ttctaaaatg gttaaaatca tttgtgcatc
     5221 atctgatata gcttcatatg ctcttggctt caatgaaaga ttcagtacac cgtctggtcg
     5281 gacaccaatc actcgacctt ttatctgctc gcctagacga ggttcattat atcgttcaga
     5341 aggatggata aacccaagat agaaatcttc cgttaaaacg tacgtacctg ttaatttcaa
     5401 gcggtatgct gttcctatca catctttgtt tttcatttct tctgttccat atttgcttaa
     5461 agatttgaat attttttcat ctgctaaact gccccaaata cggtcttttg catcaactct
     5521 aagggaaatc atcaagcgat cccctttttt aggccatagc tctgtcattg tcggcaattc
     5581 atcaagagat acgacaaagt ctttatctgg aagaccgata tcgacaaacg cacctaaatc
     5641 ttttcgtgta gcagtaacga ttccaaaagc gtaatgaccg atacggcttt taggaatttg
     5701 tgtagtgatc cgattttcat gtttttgatt ttggtaaccg aatccttcaa ctgcttcacc
     5761 gattttgtgt tcaccttctg atttgcttaa gccaaaggtt tgtccgtttt tttggacaaa
     5821 gtagtttttt tcattttcat caataataat acctgtaaat atagttgcta ataaatcgtt
     5881 catttttttc tccctctata gtgaaaggtt ttcttaatac atttctttgc tatcataaca
     5941 taatgttatc tacttgaata gctgagcctt ggtttggaaa ggtgaaaaaa ggagtatgac
     6001 aacagttgtg tcatactccc ctttttcgaa taaacggtgt taaacggcag gctcacagcc
     6061 tctttcattt gcctaaaatt aattgctccc atttcctccg aaccatccag ttttacagtt
     6121 gatggttacg atgtaaaatg gcaaagcact ttctccaatg cctttcatca atgcagattt
     6181 tgttgaagtg gcatatagat tataatagcc ttccccattt tcgattctgg ctttttctaa
     6241 tctgtaacct ccttcaacga tatatccacg ttggatacat gtagcaatca ctttgctttg
     6301 gacacctggt gcccacgtcc atgctggatt agatgcatcg gcaatatcac ttgctctggc
     6361 agatttgatt ggctgattcg ctgagtttga agaggaaggg gtcgaggatg ccgcttcttg
     6421 ctgtgctgct tgtgcgaccg cagcttgttg agcggcttga gctgcagctg ctgcttgagc
     6481 ggcttgtttt gcttgttctt cagctgcttt tttcgcttct tctgcttttt tcttttcctc
     6541 ttcttctttt ttctctttat cagtgagcat cttatctact tgtttcaatt gttcattcaa
     6601 ctgagcagat agatctgtat ttttcacttt ttctactgct tcttttgccg tcttgtattg
     6661 gtcgcgtgtt gctgaatcca ctacttttcc gtctttaaag actatagctg ttttttcttt
     6721 agctgtttgc aattcagcat attgattttt agcttctgtt tgtgcttgtt gaatcaattt
     6781 tccaaaatct gtgttatccg tttcgattgc ttggatctct ttatctgctt tcaacttgat
     6841 gtcatctgct agatgatcat cgacgatggc aggtgctatg aaccactcat tcactttttg
     6901 gatatctcgg atcttagatt ggatatcttg gtaagttgtt tctaactctg aatatccttt
     6961 ttcattctta agactggata aatcattttt tagttttgat aaatcttgat gaatcatatc
     7021 tgcacgaata aattgatggt gatcatctgt ataaaaggca gcaagtttct catgaagttc
     7081 ttccatttgt tgctgtcttt ttgcttctgc tgctacttcg gcttctactt tttgatgatg
     7141 gtcataataa gcccagcttc ctccaccgag tagaagtact gctgctaaaa cgagataagg
     7201 tacttttttt gattttttct tttgcgcttt atctggatct gttttttcta agtttgattc
     7261 ttgatgtgtt ttttcagtat ctggcacgat tgttattgat ttcgcttctg cttcaaacag
     7321 cttaggctgg ttctcttctt tttctatttt tgcttcaaga tcagtaccct ctttgcttac
     7381 agattgattt tcagcatcgg attcactatt tatttcatcg ggctctgttg actttttgtc
     7441 tgtgttttcg agtatttctt ttttctctgc ttccggcagt ttattcgttt tttccttttc
     7501 taattgtttt tcagtaaata cttcagtttt ttctgtgata tccgttttta cgtcttttat
     7561 ttttactgtt tctttacctg tggcgttttc tctatcagag tctttttcag tttcttgttc
     7621 cttttctggc tctactttgt tttcctgtgc aattttttca ctgatcattt tgagcagctc
     7681 tttcgcgtgc tcttcttttg tatcttcacg atgtgctttg atataagcgg atagtatcga
     7741 attttcttcc tcattatcga cgatttcttt cagttcagct actcttcttt tttgttttga
     7801 ttcaagcgtt tcttcttgat tctttcctga ttcttccgaa gtctcatgaa cagttgcttt
     7861 cttctgttca tttttctcta tattagttac ttgatcgtga gcaggtggtt cttcgtcttc
     7921 tggttctgct ttttcgaata attccattac agattctaga ggtaaatctt tcaattccga
     7981 ccattcgatt ggatcgttta attctgtatc aggaaagact gcatcttcag ttgatttatc
     8041 ctgcatagct gcatctgtat ttgtttcttc aattgattct tttatttgct gatcttctgc
     8101 agttatttga tgaccgcagt ctggacagat agtattctgc tccatttttt tcgatccgca
     8161 atttggacat tttttcatca cttagcgatc ggctccttta ctaggtttac gctaccattt
     8221 aatctatcat attctatacg tcacgccaat agcttatttt taggatttac ttaaaaataa
     8281 tgaatgaatt tccttcctat agaaaaaaaa gcttgagact gcgtagtctc aagctttttt
     8341 tcttttaaaa taaaccaaac agttttactg atttctcttc tggtcaatag tccacgctta
     8401 ttctaagaaa tcttttaatt gttttgaacg agaaggatgg cgcaattttc ttaatgcttt
     8461 agcctcgatc tgacgaatac gttcccgagt aacaccaaat actttcccta cttcttccaa
     8521 tgttctcgtc cggccatcat ctaaaccaaa acgtaagcga aggacattct cttcccgatc
     8581 agtcaatgta tctagtacat cttccaattg ttcttttagt aactcataag ctgcgtgttc
     8641 tgctggactt gttgcttcct ggtcttctat gaaatcacct aagtgagagt catcttcttc
     8701 accgattggc gtttcaaggg aaacaggttc ttgcgcgatt ttcaagattt ctcggacttt
     8761 ttctgttgga agatccattt ccgcaccgat ttcttctggc gtaggttctc ttccaaggtc
     8821 ttgcagcaat tgacgttgga tacgtatcaa tttattaatt gtttcaacca tatgaactgg
     8881 aatacggatc gtacgtgctt ggtcagcgat tgctcgagtg atggcttgac gaatccacca
     8941 tgtagcatag gttgaaaact taaatccttt gcggtagtca aatttttcaa ctgccttcat
     9001 cagtcccatg tttccttctt ggatcaagtc aaggaactgc atgcctcgtc ctacataacg
     9061 tttagcgata cttactacta aacgaaggtt tgcttctgcc agacgctgtt tcgcttcttg
     9121 gtcgccttct tcgattttca atgctaacgc tacttcttct tccgcagtta aaagggagac
     9181 acggccaatt tctttcagat acatacgaac cgggtcattg atttttacac cggttggcgc
     9241 agaaagatct tctgtttttg cttgttcagc ttttttctca gcactcttca agctgtgaat
     9301 tgatggatca ccattttcat ccaccacact gatacctgcg tcttcaatct tttggatcaa
     9361 tttgtccatt tcatctgcat tcaaagtgaa tggtgtagca agctgattgg ataaatcatc
     9421 atatacaact tgtcgtttgg gtttgttttc tttgatgaaa gctgctactg ctgcttcata
     9481 cgttttttta tctgttccat ttgccataaa agaaggcccc ccttgagtag ctatttggct
     9541 tgttttaact gtttcgttaa attgatgatt tcaatcgtca attcaagctc aagctgtttg
     9601 tttccagttc gtctggcttc ttgctgcatt tgttttttta atgttatttc atcagctaag
     9661 ccagatttgc tgatcacatc tagcaagtct tggatttctc gtggcgaact ttcttctgcc
     9721 accggtaaat attcgatttc caccaccaac cgccttaaat tttcatcttg caaaaagtct
     9781 aaaaactctg caaggttgaa ttcataatgt tgtgagacat atgcatcgaa taaaaaataa
     9841 acttctgcat atgcatcatg aataaactga aattctcgtt cttttaaaat attacggaca
     9901 gaaacttctt taaatgcacg atataataga atctgctctg ccttttctac ttgcgtcaaa
     9961 ggtctctttt tccgtgcagt ttcttgtctc gtaacctgag tgtgttgact ttcctgaggt
    10021 tcttgacgat tttgctgatt tgtccttcga agatcgcgca gttgtttttg tatcgtctct
    10081 ctgccgatct gaaactctgt tgacagttga gatagataca tatcttgttc gattggtgaa
    10141 acaactcggc taagttcaac tagtaattct tcgaggtatt cgatcttgtc tttttcatta
    10201 tctaaatttt tcccttgttt taaataacgt gatttaaaag taaagactgt ttcgcgccca
    10261 tgagccacta attcttggaa agatgcttct ccatacttac ggagatattc atcaggatca
    10321 agcttttctg gaacgactac gacagaaagt gctaatcgac tgttttgccg aagcaattca
    10381 atccctcgat tggttgcttc aaaacctgcg ttgtctccgt catagcaaaa gaccaattct
    10441 ttcgtcagct tttccatttg ttggatctgt tgagctgtta gacttgtccc catagaagct
    10501 aatccagttt tgataccagc ctgccatgca gcaatcacat ccataaaccc ttcgaacaac
    10561 aaaatttctc cgtttttccg gatatcgctt cttgctttat caagattaaa taaaacttgt
    10621 cttttattga acagttctgt ttctggacta ttcaagtatt tgggctgatc tttttcctgc
    10681 cctttttcag gtgcaagcca gcgtcctgaa aaaccgatcg tctggccttg agaattacga
    10741 agtggaaaca tcactctttg ataaaaacga tcagacatac ggccatcatc atgcgtgaca
    10801 aacaatccag agcgttctaa caattcattt gaaattgatt cattcttgaa tacttgctga
    10861 agaaaagaac gctcattagg tgcaaaacct atgttgaatt cttcaattaa ttctaaagac
    10921 aacccacgac tttgcaaata ttctaaagct ggctgtccag cttttgtatg caatagcatg
    10981 tgatgataga tttccgctgc tttttcatga gcagcaatca gtttccccgt agcagattca
    11041 ggattttgga cagctatagt acgctgacgc cattgatctt ctaacggtat ctgttcgaag
    11101 tctgccactt ttaccactgc ttctggaaaa cttagacctt ctagttcttg aagaaaagta
    11161 aacacgtttc cgcctttacc acaaccaaag caatgaaaaa tctgcttgtc ttctgcaaca
    11221 gaaaaagacg gtgtacgttc ttcatgaaaa ggacacagcc ccaagtaatt tttaccagac
    11281 tttttcaact ggacatattg tccaatcacc tcaacgatat tcgtttgact gcgtactgtt
    11341 tcgataacct cttgaggaat ccgttgagcc attttttcac cccctgctct attgatatct
    11401 tttgggttta acagaagata tttcaggaga ataaccggta aaaaatcgca ctaactgaaa
    11461 tcagtgcgat tttaaaggac acaattatac atgtttgatt gtatcatatt tcattgcttt
    11521 ttcaagtata acacatttct atctcattaa gcatcaattt gctactcttt tcagtaactg
    11581 attttgaata tcagccttcc agtacaatac tcgaagaaca atatttacga gcatgaatac
    11641 aaagaagtat cctacgctct ctcccaaccc taagtttact tccaaaattc catggaacag
    11701 cattgcagct acatatatcc ctacgatcgt cgttactgct aggaagatgc gcaatgggtt
    11761 gaagggataa caagctttta caactgccat gcaactgatc ccaatcagca agtaatacat
    11821 caaagtagta gtttcagcat ttccccatgc gaatgttttt ccaagtagat acaccgcaat
    11881 gatattcact actacaagca aggcatttgg taaagcatta ataagtgctg taggcaaata
    11941 acggtaagtg atttttcttt tatcttgttc aaaagataaa aagaaggaag gatacccttc
    12001 aatcgctaaa tcaatcagcg tgatttggat agggataaac ggaaatccaa ttgctgtaac
    12061 ggcacatata atcgataaaa taaatgaata aatcgttttg ataaaaaaga tgcctgagac
    12121 tttcgtcaca ttgttgacca ctcggcgtcc ttcaaacagg acttctggca atgtagtaaa
    12181 atcagagtcc aacaatacca gattcgcaat ctgacgtgtt gctccatcac cttctgccat
    12241 agcaatgctg caatctgctt ctcttaacgc caaaacatca ttcacaccgt cgcctgtcat
    12301 tgcaaccgtt ctgccgcttt ctttcaattc atttaccagc aatttctttt gctgcggact
    12361 cactcgtcca aaaaccgtat agcgatgagc agcttctcgc acttccattt cttcagtaat
    12421 ttgagaaaga tcgacatacg catcataatt cggtaatcct gcacgtctag cgatattgga
    12481 gacagtaaaa ggattgtctc cagaaatcac tttcagatcc acaccttcct catgaagata
    12541 agctagtgtt tcttgagcgt tttttcggat tggatcatcg atttcaaaca gagcaaccgg
    12601 ctcaacaaat gctggttttt cttcgttcaa ttgctgattt ggagcgatcc ccaacataag
    12661 tacgcgatag ccattcgctt gggctgtttt tagactgtcc ggaagccgat cttcttccac
    12721 caaacgttca ggcgctccta cgtacacgac tcccagttct ggaaattcca tcgcgcccca
    12781 tttgcgatca gaagaaaatg cgactttgtt tgtcacatca taccgattag aaagctcaaa
    12841 ataatctctc atagcccgca tagtgatgtt gttatcactg ctttctgcca gatacgtccc
    12901 cataatttct gggatggctt tctcgtacag cggatgtagt gcttctaccc gctggatact
    12961 cattttccct tcagtaatgg tccctgtttt atccaagcat aatgtatcga catgggccag
    13021 cgtttcaatt gaatacatgt cttgaacaag gattcgtttt tttgccagtt tggttacccc
    13081 tgtcgttaat gcaatactta ttagcaatac caaacccttt ggaagcatgc ctaacaaagc
    13141 tgctgcagat gcaataacag aaattttgat agaagcatcg cggatgaaca gtgcttccaa
    13201 aaatagtatg atccccaaag gaataatcac gaaactagtg aatttggata ctttgcgaat
    13261 cgaatctaca agttcagaac gaatagcttt gtgagtcttt gcctcgctag tcaagcggac
    13321 agcataatta tctgcaccta ctcggataac ttcagccatg acagaaccgc ttgtcaaaaa
    13381 gcttccagaa agcaattcag ctccagtttc tttgacaatc aaatcagatt cgcctgtcaa
    13441 aagagcttca tttgcttctg cccgtccaga tataacctgt aaatcggctg gtatctgttc
    13501 tcctgctccg atttttacca aatctcctaa taccagctct ttagtagaaa tcgattgttc
    13561 tttaccgtcc cggatcacta gtacttctga ttgggaaaca atcgataatt tccgtacaag
    13621 atttcttgca tgtatttctt gataaattcc aataacgata ttgacgacaa taattgctaa
    13681 atacgccatg ttggaatacg cacctacaaa taacagacaa atcccaatac caaagttcag
    13741 aaaattgaat aacgtcaata tattatcgct gaatatttgt tttgtacttt ttgatgcatc
    13801 ttcctggtag tcattttgct ggccagaagc cattttctga actacctctt gctgtgtaag
    13861 tccattcatt ttctttttct ccattttccg ccaactttca aaaataatca cgtgtttaag
    13921 tctagcgtgt tccaagatta aaaacaactt gtttttataa aatttataga aaaacacacg
    13981 aatgatttta caaaaaagat gccaaccttt ttgtttgaat cacccgtgtg ctgtttttat
    14041 tatcaatcta tctttactta tattgttcct ccaaacggct catccaccaa cctagtgctg
    14101 taccggctat aaaaagaaca ggacaagcta gtaccccagc aactcccaat cccgcgtaat
    14161 cggtaggaat gatcataatc gttgaagcaa aaactacacc taaaatgaaa tggaatagct
    14221 tgctatatgc ctttgagaaa atgtaatcca tgatttttga caatccaaga acacaaagaa
    14281 tcccaccaat tccgatcggc acaatcactt caagtcgtac ttctttaaat ccgtcagcca
    14341 tcgctttata caatcccata tacaccaaaa aattagaagg gctcaatcct gggacgatca
    14401 tacccaaacc aatcaatccg ccggcaatca gccatgttcc aaaattctga tcgacttgcc
    14461 cgtcaaataa ttgagcgccg tataataaaa acaaataagc cagaacaaaa gtaacaacca
    14521 tgatgatcac atcagaagta tctcttcctt ttttccctgc ttctttccat aacgcaggaa
    14581 cagttccagc gatacatccg acaaaaaacc ataatatcgt tgtttcatac gcacctaata
    14641 aaaaactgac taaaaaagaa agtagaaaga tgcctgtcac tccgcctaga ccaacaggca
    14701 gaaaaaacaa aacattttct ttgaaatttt ttgtaaggtg ggctaaaaaa gaaatcagcc
    14761 gctcatacat gccaaagaca gctgctaatg caccaccgct gactcctggc aagataaatc
    14821 ctgaaccgat aaacattccc ttcacaaacc gtaacatcca gtctttggtt gtgtacccca
    14881 cttcttgatt ttccattttt tgcctttttt ccactatttg cgtactcctt ctttcattta
    14941 attttattca tgattgaaca tttcctgcta ttttacttaa agactttttc ataggtctct
    15001 attatttttt cattcatttt gacaatatca ggataaaaca tgttacctcc attatgatag
    15061 agatagccgc cattgtaaag caaggactgg atccgccaat atcggtattg tttcccttcc
    15121 tcatttccta aaagtgggct cagcacatct ttagaatacg cttctgctaa ttctactgtg
    15181 ttttctccgc catgattttt tacataggca atataatctt tcccaaaatt atatgcttgg
    15241 acagctgtcc ataaatcgca tccttgtttc tttgcttcta ctatcatttc cgccaaatat
    15301 ttgacacctt gatcaatact gtcagctgga ttatcaatga tctcagcttc tccatgcaga
    15361 ctttccgagc tctgcatgag atctgtccct tttccttttg attcggtcaa tattatcgcc
    15421 ttgatcaaat tctcgtagtt ttccatatga taattttttg cagctgttgt tatttctatt
    15481 tgatagcttt ccactcgttt taccgaacga taagttcttc ctcataaaaa caacaccgca
    15541 ccaataaccg tcagaatgat catgtaaaaa agaatacgga taatagataa tcgttttcgc
    15601 ttcatttcat cacctagtta ttttgacatc tttttttatc ttactatttc tttatcttct
    15661 tggctatttc ttccttgatt tctgtgtacg ccttaagaaa aaatgaattt tcctgtattt
    15721 tttgacatct tacgaaaatg atcgttgttt ttcagtaaaa acaattgccc aatgactgat
    15781 ataatcgtta tactttacac agtaggtgat tatgtgaaaa aagaaaagaa atatgcaaaa
    15841 atgccagatg ggagcgaaat ttactatgaa aagagcggtc aaggcttccc tctcttttta
    15901 ctgcatggca atgacggcag cggccgcttt ttttcagaac aggtccctgt actcgaacgc
    15961 tactataccg tgtatctagt agatagccga ggacatggtc gttcgacaaa cgaagcatct
    16021 atgttgaatt ttcagttaat ggccgaggat ctgaatacga tcatgttgct ggaaaaaatc
    16081 gatcaggcag attttcttgg atttagtgat ggagcaaatc tagcactggt atttgccagc
    16141 agcttcccta aaaaagttca tcgattgatt ttaaattctg gaaacacgct tgttaaaggc
    16201 gttcgttttt ctgccagagt catcagtaat attcattatg catgggtatg gttactctct
    16261 ttatttcgcc caagtcttcg caaaaatctt ttagttatca aacttttgct gcatgacatc
    16321 gggcttacag aaaatgattt aaaaaaaatc aactccccca ctttgatcat cgtgggaaaa
    16381 aaagatgtaa tcaagctaaa acattctttg tatatagcta aaactatacc aaaagcttcc
    16441 tttgtgcttg tgaaagaaca aggacatgaa ttagctagaa aagatcctga acgcttcaat
    16501 cgagaagttc ttcaatttct ttctgaaaca taactattag gaatagaagg tgatttattt
    16561 gttaaaaaat accatacaat ggctgaaaaa ccacctagga ctgttcaaaa caatcttttt
    16621 gatttctgtc attgtcatta ttgtcggtga attgatgtcc atcggtaaaa ctctatctat
    16681 ccagcaatta ggagaaacgt ttgccactat acctgtatgg aaaagcggac taatgctatt
    16741 gattggtctt atctctgtgt tacctatggt tggttatgat attattttga atcggatgct
    16801 tgaacaaaag cagaatcctc gttacctgtt tgagacaagc tggctgatca atacgatcaa
    16861 caatattgcc ggtttcggcg ggtttgttag tgtggggctt cgctctgaac tatatggaca
    16921 aaagaaagag agcagaaacg tgatccaagc attatcgaaa gttttcttgt ttttgatggc
    16981 tggactttct atttacagtc tgctttcttt tttacttgta gtcttcacgc cagttgctcc
    17041 ttttttgaag caatattgga tatggttgat cggcggcggt ctatattttc ctgtcgtctt
    17101 tttatttact actttcaaaa agaaaggatt ccttggtggt acctcactta aaactcaagg
    17161 acagctattg ctagtttcat tgttggaatg gaccggtgtc ctgtttagtt tcctttctgt
    17221 tggttattta atggaaatcc ggattgatct gtggcaaaca attccattat tcattgctgc
    17281 ttcagtgata ggaatcgtgt ctatgattcc aggagaaatt gggagtttcg atgtaatgat
    17341 gattatcggg ttgtccgcta ttggtgtgcc aagagaaata gtcgttgtgt ggatcttgct
    17401 ctatcgcctg ttctactaca tcattccgtt tttgattgga atcgtctttt tcttcaaaaa
    17461 tatcggttcc acttttgatc agcgttattc tggtatccct aaacaattag caacagaaat
    17521 tgcgcataaa atcgtggttg tcttattata cttttctgga atcatgctag tactttctgc
    17581 cactatcccg caagctttta cagagtttcg ctggcttcat agtttgaacc cattgaaatt
    17641 tcattttatt atccaatttc ctagtatact attgggattt cttttaatca ttatgggcag
    17701 agggatcgca gcacgtgtga aacgagcata tttgcctaca atcttcttga ttgttctagc
    17761 tttactttat gttttattaa gtgattttag tttcacaccg gtggttttct tgtctattct
    17821 gcttctgatc attcttgctt ctaaaaacga attatttcgg gaacaattga tctattcatg
    17881 ggagtggcgt acgatcgatg gaatcattat tggtgcactg tccttgcttt acatcgttat
    17941 tggggtatac aatctgccgg atttccctca tcggcgtcac cattttattt cctttttctt
    18001 atttccttct gaaaagattt ggttttcggg attgttggcc atcatcgctg ttagtttcat
    18061 gatcgttcta tttgttcatt tccttcaagg agaaaagaaa cagattggcg aagcctttaa
    18121 tgaagaaaag gccctgaaga tccttacaac ttatggagga aactccgata gtcaattgat
    18181 cttcttaaaa gataagcgga tgtttgccta tgaaaaagac ggtgaaccaa ctgttcttct
    18241 ccaattcgct tgcttcaata ataaatgtat tgtaatgggt gatccttctg gtaagaaaga
    18301 agattttcca gaagcaatcg aagcttttat cgaagaaaca gatcgactat gctatttacc
    18361 agttttctac gaaacaagcg aagagattgt tatgattttg catgaattcg gctacgattt
    18421 cattaagatg ggggaagaag cttatgtaga cctgaatagt tttacaactt cagggaaaaa
    18481 aatgaagggc actcgtgctg tcttgaatcg gatcgaacga gaaggattta catttgatgt
    18541 cttacaacca ccattttcta ctgagcaaat gagtactttc aaaaatatct ctgataattg
    18601 gttgggttca cgaaaggaaa aagggttttc tttaggcttt ttctctgaag attatctaca
    18661 aagagcgcct attgcagttg tcaaaaatac ccaagaagaa attgtcgctt ttgcaacgat
    18721 catgcctact tacacgaaca ataaagtagg aacgattgat ctgatgcgat atgattctaa
    18781 aaaagcacct tctggcagta tggatttctt gttcttgcat ttgtttgcct atatgaaaga
    18841 agaacatatc caatggttca atttagggat ggcccctctt tcaaatgtcg gaacttccag
    18901 aaagagtttt ttacaggaaa ggattgcata cttggtttac gagtttggtt ctcattttta
    18961 ttctttccat ggattaagag aatataaaag caagtacgct acaaactggg tctcacgtta
    19021 tactctatat tctagagata gttggattgc atatgttatg attgctttgc tgattattga
    19081 taatgcgcca gttgagcaga cacaaggtag aataagtggt ttgaaaaaat ggatacgaag
    19141 aaagtattaa aaactgcata ccgatagatg aacactctca tgatgaataa aagagactga
    19201 agtaaaacga aagttcatgc ttctacaatc tcttcttata caattgaaga acaaagagcg
    19261 gtccaaacag aaatcctgtt tggaccgctc ttttatttta aacatttttc attaccaaaa
    19321 tattcgagtc gcgtttctta tgcttcgatc tctgtaacga tacctgaacc tactgtccgt
    19381 ccgccttcac gaattgagaa tgtcgttccg ttttcaacag cgattggatg gatcaagtca
    19441 acatcaatcg tcacgttgtc accaggcatg accatttctg tatcttctgg caatgtgatt
    19501 gttcctgtaa catcagttgt gcggaagtag aactgtggac gatagttgtt gaagaatgga
    19561 gtatgacgtc caccttcttc ttttgtcaac acgtacactt ctgctttgaa ttttgtatgt
    19621 ggagtaatgg aacctggttt agcaagtact tgtccacgtt cgatatcgtc acgttggatc
    19681 ccccgtaaca acacgcctac gttatcccca gcttccccgt aatctaacgt tttacggaac
    19741 atttctacac ctgttacaac tgctttttgt gtttcaggtt tgatccctac gatctctacc
    19801 tcatcaccga cacgaacagc accacgatca atacgacctg aggcaacagt tcctcgacct
    19861 gtaattgaga agacgtcttc cactggcaat agtaatggtt tatctgtatc acgttctggt
    19921 gttgggatat attcatctac tgtatccatc aattccataa tagcagcttc agcatctgga
    19981 tcgccttgca atgcttttaa tgcggaacct ttgatcacag gagtgtcatc gcctgggaaa
    20041 ccatattcgc tcaataactc gcgaacttcc atttctacca aatcgatcaa ttcttcatca
    20101 tcgactaaat caactttgtt caagaaaaca atcaggtatt ttacaccaac ttggcgtgac
    20161 aataaaatgt gttcacgtgt ttgaggcatc ggaccgtcag ttgcagaaac aactaagatt
    20221 gcaccatcca tttgtgctgc ccctgtaatc atgtttttca catagtccgc gtgtcctggt
    20281 gcatcaatat gcgcgtagtg gcgtttttct gtttcatatt ctacgtgtgc ggtattgatc
    20341 gtaatcccac gttcacgttc ttctggagct gcatcaatac tagcatagtc ttgaggattt
    20401 gctaggcctt ttttgcctaa tactgtagtg atcgcagcag ttaaagtagt tttcccatgg
    20461 tcaacgtgtc caatcgtacc aatgttaacg tgtggtttac ttctgtcata atgttctttt
    20521 gccataattt acaaacctcc taaataatat agatcactga aaaatttcag ctctcacctt
    20581 tttattatag tctttttttg actaaatcaa aaagaatacg tccgacttac tttttgtaag
    20641 attcgaacta agcttattat acgtcaatat tggtcaaagt caactaagag cactcgtctg
    20701 tcttatacgc gaaaaaagta ccttttataa ataaaaaaat gtgttttttg agaaaaactt
    20761 caagatagtc cttaactttc ttttgaagca ataagaacaa aatgaggaaa agtttaaaat
    20821 tcagctgttg ccaacaactt tcaatagacg aaatcttcta tttcgttaat aataaggaaa
    20881 gaggtgaagt acatgaaaaa attaattggc gtacttgtcg ttttaggcat atttctcggc
    20941 ggaagttttg cagcttatca ttatttttac ggcggcgaag cttattatac aaaaatcacg
    21001 acgaatggcg aaatgtcagc tacccaagca gataacggag agaaattcac gacttatacc
    21061 tacaagcaag cggcttataa taaagatggt gaaaaaaaac aggtgacgct tcgcgaagaa
    21121 agagaacatc cattaaagat gaatgcgtat cttaaactca aagtcaatcc tcgtaaaggt
    21181 gtattaagct gggaagaaat aaaggcttct gaagtgccta aaaatgcggc agaaaaaatc
    21241 gataattagt aaatggtctt aaaattttat tagataaaaa gaggctggga cagaagcgtt
    21301 taactccaag aaataagaag gagttgcttc tgcttccgcc gtttatacgt gtttagagcg
    21361 tgagacaaaa gtgttcttta cttttgtctc acgctctttt tttgttatat tcgtcttttc
    21421 cgatccttct cttgacacaa ttcatttaac tgatcactgg tacttgaaca cctaatattc
    21481 tagtcatttt tataaacgat tcaatacttc atccacattt ttgatcatca cggatatcgt
    21541 accatccgga ttattcacaa attcaatcaa atctgggttg cgatatacat ctactggtac
    21601 gatcagttcg atcccgtttg atagcttgaa tttttgtttt ccaaattttt tctcagaaat
    21661 ttcttgtact tcttttacag gaggtgcttc tgggacaaat ccttgctctt gtacttcttc
    21721 tttaaaagcc atttttgccg tgatgttttc cttgaagact ttttccgcaa tcatctcatg
    21781 atccaaccga cctttttctt ctatcgtatc atagacagct tctttcaaat cagccatgat
    21841 atcaaattct tctgtctcaa atttttttcc aagatgcttg gctacttttt ggatcacttt
    21901 tacattttct tctaaagaag gtgcaggaac actttcgatc actcttccag aaaaataaag
    21961 ctcttttttg ccagaaaact catatttttt ttcaattaat tcgtaggtcc aatcagtcaa
    22021 attcacagcg aatgcttcat ctgctttttg tgttttcgcc cctagaattg ctcgattcaa
    22081 aatcaagcgg ttgtcgatcc ctgcttcgtc tgcttcaaca aagtgagtgt aagcctcacg
    22141 ataattgact ttcaataaag ataagtacat cactgtatct aattcataca agacaacaaa
    22201 tacatctgca ctaggtgctt cttcactttc ttggtagata tcgaaccacc gctgaacgat
    22261 tgcttcactt gcttccacaa aattttcttg agcgatttta agtgattcaa cgatcatgct
    22321 ttcttctgct agttgtccag tttttgtttg agcagaagag attttttgga tcttcttttg
    22381 aagaaaatca cgaatatatt cttttgtcag atccagctct ttttgtgaaa agactggaga
    22441 accagtttct ctgtcaataa tatgcagaat tgcttttttt aaatagatat ccattttctc
    22501 attcccttct gtcttagttt acggaaaaaa aatcaaaaat accagaatca aaatcatttt
    22561 cttttaaaca aaaatttcaa gagtagaaac aaaatggatt ctttttttcg aaaaatgcgc
    22621 tacacttata gttgaacgaa taaaaaagga gctgaaagta tgaattcatt aacaagcact
    22681 tatcgtctat caaatggtta cgaaatcccg gtagtaggat tcggcacatg gcaaacacct
    22741 gatggggatg tcgctgtttc ctctgttaaa gaagcgctgg ctgcaggtta tcgccatatc
    22801 gatactgccc aaggatacaa gaatgaagaa agcgtcggcc aagcgattaa ggaaagtgga
    22861 attcctagag aagagatatt cttgacaaca aaattatgga atgcaaacca cagctatgaa
    22921 cttgtgatgt cttcttttga agaatcattg aagaagctcc aaacagatta tttagatctg
    22981 tttttgatcc actggccaaa ccctgttgct tttcgtgata attgggaaca agcaaatgca
    23041 gatacatggc gagcatttga agaactgtat gaagcaggta aaatcaaagc aatcggtgtg
    23101 agcaatttct taccccacca tctagataca ctagcaaaaa cagcaaaaat tatgccgatg
    23161 gtcaatcaag ttttccttgc accaggcgaa ttacagacaa ctgtagttga gtatgcaaaa
    23221 aaacatgata tgatcctcga agcatatagt ccattaggca ctggtaaaat cttcgatgtg
    23281 cctgaaatga aacaaatcgc tgaagcacat gacaagacga ttgcacaagt tgctttgcgt
    23341 tggtcgctgc aacacgaatt ccttcctctt cctaagtcag tgacaccaag cagaatcaaa
    23401 gaaaatactg aattatttga ttttgaatta acagaagaag aaatgaaaca aatcgatcag
    23461 ttagatggtg tagtcggcaa agcaaaagac ccagatacta cccaatttta agtcgaaagg
    23521 tgattttatg gatccaatcg aacgcttgaa ttctctctcg gaagaagtta cccagacttt
    23581 tcattccgat tttgtctttt tgatcgatgc agagaaaatc caacattttc ctgcaagaaa
    23641 ctggacacat gatcaaatca tcgaagaact gaaaaaacgg ttcgatcatt cgctaatggt
    23701 caaaccctgg cacgaacatg aagtcattta ttcacctgaa ctacctgttt ttgccttgat
    23761 accgaaaaaa tagaaagaat ggaacggatt atgtatatat acataatccg ttccattttt
    23821 tatttttctt tcaaaaaacg ggaaaatacg caaatttttc tgttgtaatt tgcgtatacg
    23881 caagtttttt tgcattactc cttttcattt ggctgttttt ttcttataat cgaaagggta
    23941 tgagcgaagg agagaacata tgaagaaaaa acagaaacaa aaattattaa atcaattccg
    24001 tccttcattg gatggtgtaa gaggacaatt attccgttct ctagaagaag agagcttgcg
    24061 acgttatggt ttgccgttac aagtcatgct tgatcccaaa aaccgtcaaa tcttaacgat
    24121 cggattagct gggaaaacaa atgaagatac acggatcaac gtgccaatcg atgacaactt
    24181 caatactgta ttaaagagaa ttcgttcagg agaaaacggg attttcgaac gattccgtga
    24241 caatcttctt attgaaatcg tgtcttactg gaatgatcaa cgattgaaaa acgatgaacc
    24301 aactgcgcaa gctgttgcga caccagttac agaagaaaca gtcagtgtgc cagaagaaaa
    24361 aacggaaaaa ccaaaagcgg acacaactga aaatacacgc tcaactgata cgaatgccgc
    24421 tttaagtttt gctgattttt caaaagccgt tgctgagtat cctaaatttt acacagaaca
    24481 aaatgaacat gaagtagtta ttaatgaaaa aaatgctgat gaacctcgta aattagcaac
    24541 tatttctatg actactgaaa atgagttcgt tatcgaaaaa gcactcgaac ggaaatacaa
    24601 agtcaaattg actttgattc ctttgatcga acaatttgca gcgacagcga ttactgatcg
    24661 ataaaaattc tatttgtcat aaaaggctgg gatttaaaat cccagccttt ttagtcatat
    24721 aataatttct acagcaagta aaaagaaatt ccttctaaca tcaggctcaa atatcagctt
    24781 gacctgtcca acaaatcacc tatgatccta caatgttagg cgacttctct tctacctgaa
    24841 gatatcttgg agttgcctga tttcttaaga tgcctaacct taactataaa aaattctaat
    24901 aataaacata ttttctgata gagaaaaaa
//
LOCUS       DASJTC010000035        24722 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.035, whole genome shotgun sequence.
ACCESSION   DASJTC010000035 DASJTC010000000
VERSION     DASJTC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.035"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1835)
                     /locus_tag="U4B19_001289"
     CDS             complement(<1..1835)
                     /locus_tag="U4B19_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2077038.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="U4B19_001290"
     CDS             complement(1832..2536)
                     /locus_tag="U4B19_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2077039.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="U4B19_001291"
     CDS             complement(2548..4857)
                     /locus_tag="U4B19_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN2077040.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="U4B19_001292"
     CDS             complement(5052..5516)
                     /locus_tag="U4B19_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077041.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="U4B19_001293"
     CDS             complement(5516..6142)
                     /locus_tag="U4B19_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2077042.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="U4B19_001294"
     CDS             complement(6149..6514)
                     /locus_tag="U4B19_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN2077043.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="U4B19_001295"
     CDS             complement(6504..6842)
                     /locus_tag="U4B19_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077044.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="U4B19_001296"
     CDS             complement(6832..7158)
                     /locus_tag="U4B19_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEN2077045.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="U4B19_001297"
     CDS             complement(7139..7417)
                     /locus_tag="U4B19_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEN2077046.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="U4B19_001298"
     CDS             complement(7419..8783)
                     /locus_tag="U4B19_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEN2077047.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="U4B19_001299"
     CDS             complement(8796..9365)
                     /locus_tag="U4B19_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEN2077048.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="U4B19_001300"
     CDS             complement(9337..10524)
                     /locus_tag="U4B19_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2077049.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="U4B19_001301"
     CDS             complement(10588..12315)
                     /locus_tag="U4B19_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEN2077050.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="U4B19_001302"
     CDS             complement(12312..12764)
                     /locus_tag="U4B19_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEN2077051.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="U4B19_001303"
     CDS             complement(12876..13256)
                     /locus_tag="U4B19_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEN2077052.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="U4B19_001304"
     CDS             complement(13253..13639)
                     /locus_tag="U4B19_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077053.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="U4B19_001305"
     CDS             complement(13641..13919)
                     /locus_tag="U4B19_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077054.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="U4B19_001306"
     CDS             13967..14695
                     /locus_tag="U4B19_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HEN2077055.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14826..15302)
                     /locus_tag="U4B19_001307"
     CDS             complement(14826..15302)
                     /locus_tag="U4B19_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEN2077056.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15262..15609)
                     /locus_tag="U4B19_001308"
     CDS             complement(15262..15609)
                     /locus_tag="U4B19_001308"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077057.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            complement(15596..15781)
                     /locus_tag="U4B19_001309"
     CDS             complement(15596..15781)
                     /locus_tag="U4B19_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077058.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15782..16054)
                     /locus_tag="U4B19_001310"
     CDS             complement(15782..16054)
                     /locus_tag="U4B19_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HEN2077059.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16051..16254)
                     /locus_tag="U4B19_001311"
     CDS             complement(16051..16254)
                     /locus_tag="U4B19_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077060.1"
                     /translation="MVKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            16379..16591
                     /locus_tag="U4B19_001312"
     CDS             16379..16591
                     /locus_tag="U4B19_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077061.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16624..16818)
                     /locus_tag="U4B19_001313"
     CDS             complement(16624..16818)
                     /locus_tag="U4B19_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077062.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16831..17013)
                     /locus_tag="U4B19_001314"
     CDS             complement(16831..17013)
                     /locus_tag="U4B19_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077063.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17010..17150)
                     /locus_tag="U4B19_001315"
     CDS             complement(17010..17150)
                     /locus_tag="U4B19_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077064.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17640..18005)
                     /locus_tag="U4B19_001316"
     CDS             complement(17640..18005)
                     /locus_tag="U4B19_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077065.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18045..18347)
                     /locus_tag="U4B19_001317"
     CDS             complement(18045..18347)
                     /locus_tag="U4B19_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEN2077066.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18347..18646)
                     /locus_tag="U4B19_001318"
     CDS             complement(18347..18646)
                     /locus_tag="U4B19_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077067.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18639..18803)
                     /locus_tag="U4B19_001319"
     CDS             complement(18639..18803)
                     /locus_tag="U4B19_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077068.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18809..19078)
                     /locus_tag="U4B19_001320"
     CDS             complement(18809..19078)
                     /locus_tag="U4B19_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077069.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19090..19839)
                     /locus_tag="U4B19_001321"
     CDS             complement(19090..19839)
                     /locus_tag="U4B19_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2077070.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19836..20528)
                     /locus_tag="U4B19_001322"
     CDS             complement(19836..20528)
                     /locus_tag="U4B19_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077071.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20534..21205)
                     /locus_tag="U4B19_001323"
     CDS             complement(20534..21205)
                     /locus_tag="U4B19_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEN2077072.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21198..21539)
                     /locus_tag="U4B19_001324"
     CDS             complement(21198..21539)
                     /locus_tag="U4B19_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077073.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21717..22415)
                     /locus_tag="U4B19_001325"
     CDS             complement(21717..22415)
                     /locus_tag="U4B19_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEN2077074.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22502..22972
                     /locus_tag="U4B19_001326"
     CDS             22502..22972
                     /locus_tag="U4B19_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077075.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23015..23194)
                     /locus_tag="U4B19_001327"
     CDS             complement(23015..23194)
                     /locus_tag="U4B19_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077076.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23181..23519)
                     /locus_tag="U4B19_001328"
     CDS             complement(23181..23519)
                     /locus_tag="U4B19_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEN2077077.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(23535..23738)
                     /locus_tag="U4B19_001329"
     CDS             complement(23535..23738)
                     /locus_tag="U4B19_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2077078.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(23753..24529)
                     /locus_tag="U4B19_001330"
     CDS             complement(23753..24529)
                     /locus_tag="U4B19_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEN2077079.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
BASE COUNT         6861 a         5171 c         3817 g         8873 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttagcc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtgataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta cgaacattta gtgaacagtg
    14821 taattttaga ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg
    14881 ttttcttttt tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc
    14941 tttctaattt ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca
    15001 atatcttcta agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca
    15061 gaaaagctat ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca
    15121 ttttctatct tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg
    15181 acgtttgcta aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc
    15241 cactttaagt aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc
    15301 acaaacgcca ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt
    15361 tttatttttt cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct
    15421 tcttaatctt tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc
    15481 gatttttaat actaaataaa agttggccag aatttttcgt tcgtctttac tcatcttttt
    15541 ttgaataaga ttactagcat aaaaatagtt agtaccacga aaatgaacag gtaaatcaat
    15601 tacgttcacc ttcttcatgc cagttatcag ccactagaat ctttgcgtta gtgccgccac
    15661 cgttttcttt tttgtatttt tcaaatgctt tataagcagc tgtttttgat ttatagattt
    15721 ttccggaacc gactgggaac atccatgtgc cctctgtgat agctaagatg tatactttca
    15781 tttaattcac ccctttaatc cccaacacta catatccgga cttctgcatg taatcagtaa
    15841 tataagtgat ctctgctttg tatgagtcac cagtgtaact aaatgttggt ctcgttagtc
    15901 caggtaattc atgtttaatg tattttttat attcacgtag tattaactga tcaccaactt
    15961 tgaagtcacg atcattcttg cggatctcga attgtttgcg tccgctcgtc actgcttcaa
    16021 aatattctgg tagtattttt agttcgtgga tcattctgtt tcctccttca aaattcgctc
    16081 atcatcaaaa agtgaatctc cacaaaatcc ttctcccaaa tatttatcgt tcatgtataa
    16141 gtcaaccaaa tatttgtgat ctgcttctcg atattcatta atattaacga tctttatttt
    16201 gtaaacatta ccttcgtcgc tcactatctt gaattgttct ctgactttca ccaccgttcc
    16261 tcctcttttt gaaaaaattt tagtgttttt gtaagacccc ctaaatcagc ttttttattt
    16321 gatataatac ttgtatcgat taatcaaatc gaaattaaat ttaaaaaggt gatttatcat
    16381 gaatatcaac aatttagtaa aactcgttga acaacgtttt aaagaatcag gattagaaca
    16441 attaaaaaaa gataattctc cagaattatt ttctaatttt accgaagacg acattcctac
    16501 tcttaaacaa ttaaaatatt attctgatta ttggacgcaa actcgtgaga atgaattaaa
    16561 tttagaaaaa ctcattatta aaaagaaata gcaactccag cccacacggc tggtttttta
    16621 gtttcaactt ccctccttac gggtcaaaat catcggttcc ggcataatct gatcatcctt
    16681 tagcaacaaa tcaggatatt ttttccttag cttcttattg ttcgtgtaag ttttcaatag
    16741 ccattggttg gcttcgcttt tgctttctcc agaaaaataa attttccctg tttggatgcc
    16801 tgcaagttga atcatcattt tcacctcaaa tcaaattttt tctgctagat ggagcaaaag
    16861 caatccgaca tcaagaatct tttgacccat tatttcagct actttgctcg gcttaactcc
    16921 ttgagaaaag agcaagcgtg cttgctctat ctcttcgtga gtccaaataa atttggcttc
    16981 ctcaaggata attactggat cagttgctct cattgttcct ctggaaaaat gatgtcattc
    17041 ttatgcttgg accaggtatc tgcatatgga acaaagtatt gtctagctaa ttcgatttga
    17101 tcaattaaag cagtttctga taattcatgg tctgcagcaa tttcattcat actatcgcct
    17161 tcatctaaac agataagacc gttgacgtta tcgattttta catttgtgac tgcttgactt
    17221 tctccacgct cataatattc tacagttgat actgatacat cagcttcgga gtttaattgc
    17281 agaatctgtt actgccggga taatttaata ttcttttcat aattcacctt ttttcctact
    17341 gacatcgaat atttttcatg ttatgattag ccgtgaaagg agagctatat tatgtgttta
    17401 cacatgatgt ccttagctca ttcggttctt tggaagcttt tttggaaata actctcaggt
    17461 tcttgacgca aatctcattt attctttgaa acaaaaaact tccattttcc gagagctttg
    17521 gatcacttca aaagagattt gcatttatat ttctgtgacc ttgagtcgcg taaagaacct
    17581 tacagcaatg gcggcaattc cactttcagg aaaccgcctt ttttcattga tattggtggt
    17641 tagcggacta cccattttct ttcggcagtg ccaaaccaca aatgatctgt gttgatgttt
    17701 aggtagttgt ataccagata agctaaatcc aaagtgcctg catcttcaag acgacaatat
    17761 tccaaaaatt ctcccatagc tggacataaa aatcctggag ctatcatatc ccaacagtta
    17821 atagtgcaaa gaaattcgtt tagcttctga acgttttctt tggtttcacg aattacaaca
    17881 tactccaatt cttctccgct ggcagagcat tccaaaactt caaattgatt aagaaatggt
    17941 agaacatatg cttcaaacat caacattcca tctttctgtt tttgcacttg ttttgcatca
    18001 atcatgtcta tcctcctatt cagttatttc ttccattaat ggaattattt caggtcactg
    18061 gacttaacga aaacaccatt gaccatcttt ccagtccgac ctttgatttc gtcataagca
    18121 aaatccaagc actcgtataa atccatatca ttttgcattg ccaagatgat caaggttact
    18181 actacatcgc caattccgtc tcttaaacca tgttcatctt ttctagctag agaagcggca
    18241 acttccccga tttcttcgac cgtttttaac atttgcttgc tgaaatccgc ttgatccaat
    18301 cccttatctt ttgcccactg ctctactttc gcgattagtt cgtccattat ttctcctcca
    18361 catacctaaa ctgtcttcct tttgaatcaa tccataagct cctagctcta tcccaaataa
    18421 tatttttgct cagaccagta atttcagata actgtttagc ggtacccgtt actagaattc
    18481 gatcaccatg ccagattgca atctttctcg gcgttctccg tttgggcttt tcagtccaca
    18541 ttgatttacc gagcttttgg acttctgcaa ctattttttt atcttcctgc caatcttctg
    18601 aataagtcaa ttcgataatt cgctgcattg ccgctttctt atccatcccg acattctcct
    18661 ttcagtaatt tgagtacttg atcaagtgcg ctctcacgtc cgccatgaaa tgtgttgagc
    18721 cacttgtctt cgtacgacac actttgtctt aaagcttctt gatgcattaa ttcgatctgt
    18781 gctgtaaatg tctttaggtc catcttgttt acacctgctc aagttcatta agatgttttt
    18841 gcaagccttt aacacaatca atgaatagta atttggtata tgctaaattt cttaattgcg
    18901 ttccatcgat atagagtgcg aaatagtatc tgagtttact ccaactcgaa cgatcattct
    18961 taattccttc aattccagct tcttcgagtt gatcatatac gtctctcagg acgtctaatt
    19021 cctcgccctt tttataattc gctatttcat taattagttc tagataatcg atcttcattt
    19081 ttccacctct tagaatggtg cttttgattg tctattagct cgttctagcg cttttttctt
    19141 gagataggct tcttggtcga ttgcccattc aggaagcttc tctcgtcttc ctgtgcgctt
    19201 gtatccactg cttgcgttct taggttcact tttttctttc cttgcccaac ttcgaatagt
    19261 tgccaaatag tttttataag tcttaccaga tgattcacaa tactctgaca gtcgttcgat
    19321 tcgctcttgg taatcattag ggaattctgt tttgagtttc tccatctgct catctgacaa
    19381 aagaacattt ttatactctc cgtatttatg acggacgggc ttagccttcg attttttcga
    19441 aggcggtaac tctcttatat attcttttgt attattaaat gtattattaa tagatgtatt
    19501 attatctttg actttttcgt caataggggt attgcgtttt tcgtcaatag ggtattgatt
    19561 aattcgtagg tacctattga ttatttgatt ggtaccctct ttgtaaatga tttcccgatt
    19621 caagtatcca aacttaatca aatcacttac ccatcgcgat atggtctctt tattcacacc
    19681 atataaatct gcaaagtact cattgcctgc ccaacaaaag cctctttcat tacacaaggc
    19741 cgttatctct ccgtataaca acttagtatt tggtttaagt cttttgtcgt accttacgtt
    19801 ggctggtata atcgcataat aacttcgatg ttctgtcatt tttcccctcc aatatttaac
    19861 tttttgattg ttttctggtt taatttgatc cctttgattt gatatttatt tttgaaatta
    19921 atcacaccta ttttgtgctt ctctgtgtga tggattctgc agagtgctgc aaatgtgtac
    19981 tctgcatgat caacttcttt gcgctttcgt cttcctagcg ctttgtcaaa gtgatcgatg
    20041 tcagctcctg ttttgccaca gatgcagcag actctttttg tgatgcattt gtagaagtaa
    20101 tattcttgat tcgcaggtaa aatctcatag ccttctttga aaggaatatg atgttcaaag
    20161 atgaaatcta agatgatatt tgctaagaca ttggcatcac tcacagttgt attcgattcg
    20221 tctttcaggc ttattttgcg ccctgtgaca ccttcaaaac gaaagtagaa gaattccttc
    20281 cagaagtccg ttggcatgcc tgtatcgata aaaatatcgc ctatcagcgc atagatgaag
    20341 tttcgttgct gtacggtgaa acgtctagga tcaataaaac gaatttcaat gactcgatca
    20401 ccatcgtagc cgtcatacat cgtctttagt cgatcaatgt tcacttcctc attgatggtt
    20461 gcgcttatgt cttttccttt gaactttttc agaaccgctg aatatgaatc gattaatggt
    20521 ttaaacactc atatcacttc ttatctaatt cttttctctt agctgctatt gctcgctcca
    20581 tcaaggcaca ttgctcatag cttaactgtt caatagtttc aacgttatca gctaagagcc
    20641 ctaatttatc tgtctgctca ttaacatatt cgattaaggt tttggtcata tctttaccca
    20701 tctgctcatt gaaagcttct agaatcgtct ctagcatgtt taatttcttt gtatcgattc
    20761 taggtggtgt tggaatatct tccccttgaa atacatataa tcccagtccg tgtagagcca
    20821 atgctttcac aaagcatcgc ttcaatgagt tattgatttg cattgcattt ggtttaacaa
    20881 ctggttggtt tcgataatct aaaacaggaa ataattcggt ttccgtgtgt cctttaaccg
    20941 ttactgagac agatacataa gtcccagttt catccataag aaaaggttta tattcctcaa
    21001 caagaaagtc ttgatgagtt ccagaaacaa ccctgtagtg tttgtactca ttaatagtta
    21061 ccgttgcctg tggatcattc tttttcataa tctcccacgc gtgagcccaa gataaataat
    21121 caaaatttcc ttttttcttg agaattttat ttaacttacg actaaaaagt ttttcaaagt
    21181 tcgttgtccc tttgatttca ctcatcaaat tctgcctcca tttcagcaat gtatttctta
    21241 cctggtccgt aataagagat atcaatcaag ttatctctgt cgtactcttc tagcgcatca
    21301 atcaagccat cttcgatgac gtaaatatat tcaggtttat tcgaatgctt cgatagatgg
    21361 ataagataaa catgatccca aatagttaca tggttgccta aatcatcttg atcccaagct
    21421 agttcttcat tcgtcaaaag atttcgtctg atttttcgac cacttgtttc ctcaattttc
    21481 ggcttgcccc aatcaggatc aatcaaatat tgatctagag tggaaagttc tttttccata
    21541 tgctaaaatc tccttatgat gtgttttctt tgtgactctt tgcttgccgg cggagtcact
    21601 tttttatttg ttgccatgct ttttgcttgt caatatgttg ttggcttagg atgattggtt
    21661 tatagtattt ccaccagcaa ttagcaattg ccgtccctat tcttagcgct tcagctctat
    21721 tcattgtcat caccaaaaag tctctgttgt ctgttcagtt gatcaatttc catacggatc
    21781 gcagtttctg gcaaccacat ttcaataaat gaaacagcat catcgaatct cttacgaggt
    21841 aactcgccat atcttgggat tgaaaaggta cgtttaaatt cagaccaaaa ttttgagaat
    21901 acttttttgc tgatttcttc ataagctcgg ctttcttttc cccctagaac tcccataact
    21961 ttcatatttc ctttttgctt aatttcaaac tcttgttgtc cgctaattcg catagtatct
    22021 ttaagcatgg aaacatcttt tttaacatct ttcatttctt ctaattgata gatcatcata
    22081 tcttcaattg tttgaggaac agtattcttg cgaataacat cttccatttc gttgaatgct
    22141 tcaatgtatt tttgtttgaa gtagatagct ttctttcctg taaaccccat agccagcaag
    22201 aaaaatccat ctctactaat gaagaaaact cgtcgatttc tgccatatga atctggttca
    22261 ttaccttcta caaacatctg tccaaaattg gacacatctt tttttagtgc atcaatatct
    22321 cttaaaacat gttgatgttt tttctcgaag ctttctgcca cttgtaagct cgtagtcaca
    22381 gcttctttat ttttcaaaat tactaattct tgcattattt cttctctcct ttttggtata
    22441 atttagttaa aaactggtgg tgtttatttt gaattttttt cacttaacaa tttttcaatg
    22501 gatgtcaata ataagtttta tattgagtac gtttttaaca gggataaaaa taaaaggaat
    22561 gcgccctcaa ctagaagttg aacttaatgc ttcttacttc gctgcagata tgatttatac
    22621 aaaagtaatc atttctaact attcaacaga accagctatg ttagttaatt tagaactttc
    22681 atccgctaac ttaaaccgca gatggtcagc tactccattt aagaaattaa ttgctaaagg
    22741 aggatcagtc gatgataata gaatatactc tgaagctgta ccgctaaata ttcctcctaa
    22801 aagtgctata tcttgttacc ttgcatttga agtagggaaa attaatttta ataaactcct
    22861 taatcaccaa acaaaaatga tatttactct aaatagaacc caaatccaca aaattgttga
    22921 tatcaaaaat acaaacttcc caatagagaa attagtgaaa gaattaaact gagtaatagt
    22981 aatatacatt ctagtttctc ttttctattc atttttagtc agccccctcg gttggctttt
    23041 tcgctctgta ctcagcttca gcttcatcaa gccccataaa aatccaaacc atgtaaacaa
    23101 tcgttcctat caacgcttgc ctgcttcccc aaagtcctaa agcgtagatg attagcggtg
    23161 cgctgaacac tagcgctcta ttaaattttc ccatccgctt gcctccttaa gatttccgaa
    23221 aaatttgttt ctaaaaattc aagtgtttta cttcttaaaa atagatatgt gtctcttcct
    23281 tcaactggat aatagacaaa tccatttttg tttttctcga tatcgataat atttctatat
    23341 cttgggttct ttaaaactct agaagtaaac caatcatatt ttctgttaat ccgttctagc
    23401 acttctggca acgtcatcca tctaccagta tcatcagctt tttttaactc ctcataatcc
    23461 acttgggaga taattacata gccttctgga attgggattt ttgcttctag atattgcatt
    23521 agctgttcct ccctttacaa ttcgtacata gtgataatcg aatctataat tctgtttgct
    23581 tctgcagaag tctttttacc gtttaaaatt aaagataagt agcttttgct aattccaaat
    23641 ctttcagcaa gcatggtgta agttaagaac tttgaacttt cgacatattc tttgattttt
    23701 tctctatctc gttgagtgat ttctgcaatg tcagtcatac taaaactcct ttctaaccaa
    23761 tttcctctaa atccatttga gggtaatatc cttctttttt tagtaattcg tagataaata
    23821 gacgcccttt ctgtgtccat ttggtattca ttacaatttt agtgccacca tcggctttcg
    23881 ggatctcagt tgtatgagat tttgtgtatc cttggttcat gtgttttttg cacaataacc
    23941 attggttacc gacttttttc tgaataccta gtttatgaag taatttattc atctgttgtg
    24001 gagacatccc ataatctgct gcaatctgac taattgttac tgaatctgta gaagataata
    24061 tgctatctaa ataggagatt ttaggttcgt actctgcaat cttttgttct gcgattaatc
    24121 ttccagttct ttcttctttt agttgagttg ctaattgaat gattgtatct ggattaagca
    24181 aagcttcttc tactttttct ggagttagat aacctccatg ttttctaatt gctggcaaca
    24241 cttcacttgt tacccaacgt ttaaattttt tggcagaggg aagttttgat tttaagatta
    24301 aactgtacaa accagattcg ttgatgattg tcatctctct tgattgacct gaggtcgtga
    24361 ttcgcgaccc catcttatct tccaaatcta catgccgcga caaagcatct ttagtgtttg
    24421 aataacccaa aacactcgca acgtctttgc ctacaaaata tggttcatca tttactaaaa
    24481 ttgtccgaac ttcgttttgt tcgaaattaa aaatttgcgg tgtgttcata tttctcattc
    24541 ctttctttta tattcgtaaa caaatttaac aacttttttc taaattcaat tgacagtatc
    24601 tgagtaatac tctatactaa gagcataatt aaataagaca taaaaacatt gattttaaag
    24661 ctttcttggc ggtctgcatt tacatatcaa tagtgttttt tgttgtcttt ttagttgtta
    24721 aa
//
LOCUS       DASJTC010000036        24566 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.036, whole genome shotgun sequence.
ACCESSION   DASJTC010000036 DASJTC010000000
VERSION     DASJTC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.036"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(39..1145)
                     /locus_tag="U4B19_001331"
     CDS             complement(39..1145)
                     /locus_tag="U4B19_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN2077080.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            1355..2356
                     /gene="ccpA"
                     /locus_tag="U4B19_001332"
     CDS             1355..2356
                     /gene="ccpA"
                     /locus_tag="U4B19_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEN2077081.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(2693..5038)
                     /locus_tag="U4B19_001333"
     CDS             complement(2693..5038)
                     /locus_tag="U4B19_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEN2077082.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            5317..6219
                     /locus_tag="U4B19_001334"
     CDS             5317..6219
                     /locus_tag="U4B19_001334"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEN2077083.1"
                     /translation="MLLGSHVSMSGKKMLLGAAAEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            6432..8207
                     /locus_tag="U4B19_001335"
     CDS             6432..8207
                     /locus_tag="U4B19_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEN2077084.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(8273..9859)
                     /locus_tag="U4B19_001336"
     CDS             complement(8273..9859)
                     /locus_tag="U4B19_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEN2077085.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETVEEIE"
     gene            10110..10334
                     /locus_tag="U4B19_001337"
     CDS             10110..10334
                     /locus_tag="U4B19_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEN2077086.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            10475..12226
                     /locus_tag="U4B19_001338"
     CDS             10475..12226
                     /locus_tag="U4B19_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2077087.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSVKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTK
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIVMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPVKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            12226..14010
                     /locus_tag="U4B19_001339"
     CDS             12226..14010
                     /locus_tag="U4B19_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077088.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRTTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVLVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            14336..15187
                     /locus_tag="U4B19_001340"
     CDS             14336..15187
                     /locus_tag="U4B19_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2077089.1"
                     /translation="MLIEKVLNNNVVITRDERDREMIVMGKGLAFKKKVGDPILDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVHLNDSVYISLTDHLY
                     TAIERQKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GAKSEDGEVSEELFQVIQKKYKDTFGCVEKIADFLTGAFDYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            15283..17163
                     /locus_tag="U4B19_001341"
     CDS             15283..17163
                     /locus_tag="U4B19_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2077090.1"
                     /translation="MGKYQELAENIVKNIGGKENIRSLTHCVTRLRFKLKEENKAKDD
                     VLKKMEGIVTIMKSGGQYQVVIGNHVPDVYAEVLTVAGISDEAEEEVSSSSNLFNRLI
                     DILSGCFQPFLGALAAAGMVKGLNALFLFLKFYTEGSGTYIMLNGIGDAIFYFMPVIL
                     GYTAAKKFHLNPMVGLVIGAALCYPTVQGAALQSGFPDGSPAPYSLLGLPAYETFLGI
                     PWVGSSYTSSVVPVIFIIAFAGQVQKIAKKWIPAVVQTFLVSFSVLLIALPAGFLIIG
                     PIVSILTDLLSTGFQALMDFSPAVYGAVLGFLWQVLVIFGLHWSIIPLAIMQLAQNGW
                     SQMLVGVYGASFAQTAVVLAMYFKFKDQKMKALCMPAVISGIFGVTEPAIYGITLPKK
                     KPFIYSMIGGAVSGIYLMASGTISYIMGGLGIFGVVNYITETDASGMFQAFIGIAIAS
                     VIGFSLTFFFWKDEAGETDAIQKKSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGRGI
                     LIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESLVQ
                     QGERVRQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGETVGPDDALITA
                     LT"
     gene            complement(17203..18105)
                     /locus_tag="U4B19_001342"
     CDS             complement(17203..18105)
                     /locus_tag="U4B19_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2077091.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKYLLLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNDVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            18336..19739
                     /locus_tag="U4B19_001343"
     CDS             18336..19739
                     /locus_tag="U4B19_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2077092.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLSADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            19739..21172
                     /locus_tag="U4B19_001344"
     CDS             19739..21172
                     /locus_tag="U4B19_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEN2077093.1"
                     /translation="MGFRKDFLWGGATAANQCEGAYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDVFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSNN"
     gene            21317..22243
                     /gene="trxB"
                     /locus_tag="U4B19_001345"
     CDS             21317..22243
                     /gene="trxB"
                     /locus_tag="U4B19_001345"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEN2077094.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            22457..24184
                     /locus_tag="U4B19_001346"
     CDS             22457..24184
                     /locus_tag="U4B19_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEN2077095.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            24199..24549
                     /locus_tag="U4B19_001347"
     CDS             24199..24549
                     /locus_tag="U4B19_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN2077096.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
BASE COUNT         7723 a         4415 c         5270 g         7158 t
ORIGIN      
        1 aaaaggtggg agcggactag ctctcacctt ttttactctt atttttctaa aacaagtaat
       61 tcttttggtg tagtcgtgaa tggcacacag ccatcgcttg tcacatatac acagtcttcg
      121 atacgtaccc ctactttttc aggaatatag attcctggtt caagagagaa gcacatccct
      181 tcttcgatta ctaagtcatt tccttctaca agggaaggga attcgtggac ggttgttccg
      241 atcccgtgac ctaaacggtg attgaaatat tcgccgtagc catagctgtt gatcacgttt
      301 cgtgcgattt gatcaagttc acctgctgtc acacctggac ggacggcttc ttgtgcggca
      361 agctgtgctt caagagtgat gttgtagatt ttttcttgga agtcggttgg tttttgataa
      421 gcaattgtac gagtagcatc actgcaatag ccattccaaa caacgccaag atcgaataac
      481 acaagttcat tcggtgaaat cttcgtattt cctggtgtac catgagggct tgcagcattt
      541 gctcctgcaa ggactaatgt gtcgaaagac atttgtgaaa caccttgttt tttcaattgg
      601 tattcaattt ctgcaatgat cgcttgttca gtcacgcctt ctttcactgc tttaaagcca
      661 atctcaaatg caacatcggc ccatttacca gcttcgatca gtttgtcttt ttcctctaga
      721 gttttgatca gttgtagacg ttggatgatg gaagttagat cagtgtaaga atccgttccc
      781 gtcaagatat cagagatagc atcgaagcgc tcgacagtca aggcgttctt ttcaatccct
      841 acattgccag gctgaccata gcgagcacga agttcttttc cgatgatctc ccaagggttt
      901 tcgctgtcta aatagccacg gacatcatac gcccaactac tgttttgagc atcttcaact
      961 tccaaagcag gagtgaaaag gaaaggatct tgttcaagag ggataaaaag agctagtaca
     1021 cgctcatgag gatcactttc gtatccggaa aagtaagcga tgtggccagg gtcgctgata
     1081 taagccaatt cgacttggtg ttgtgccatc cattgacgta gttcattgat ttttacgtta
     1141 ttcatttggc aacctacttt ctaaattaat tttgtattca ataattgtac cactgattta
     1201 ttaaaagacc aacgttctaa aaactgaaaa aagtaaaaat tttcatgaaa acagagataa
     1261 aacggttgca attcttgaga ttatttgata ttctatttta gaaatgtaag acgtattttc
     1321 agaaaacata taaaatatga ataggagaac acaaatggaa aagcaaacaa tcactatcta
     1381 tgatgtagcg agagaggcaa atgtttcgat ggctactgtt tcacgtgtcg taaatggcaa
     1441 tccaaacgtt aaaccagcaa caagaaaaaa agtcttggaa gttatcgatc gcttggatta
     1501 tcgtccaaac gcagttgcgc gtggcttggc aagtaagaaa acaacaacag ttggtgtaat
     1561 catccctgat gtcagcaata tgttctttgc ttctttagca cgtggtatcg atgatgtggc
     1621 aacaatgtac aaatacaata ttatcttagc aaattcagat ggcaacgacc aaaaagaagt
     1681 caatgtattg aataacttac tggctaagca agtggacggc gtgatcttca tgggtcacca
     1741 tatcacagac gaaatccgtg gagaattttc acgttcaaaa actcctgttg tattagctgg
     1801 ctctattgat ccagatgaac aagtaggtag cgtaaacatt gattatgtat ccgcaacaaa
     1861 agatgcagta aacaaattgg caaaaagcgg caataaaaaa atcgcttttg tcagtggtgc
     1921 gttgatcgat cccatcaatg gacaaaatcg tctgaaaggc tataaagaag cattaagcga
     1981 aaataaccta ccatatagcg aaggattgat ctttgaagca caatacaact tcaaagatgg
     2041 tctatctcta gtcgatcgga tccataatag cggtgcaaca gctgtctatg tttcagatga
     2101 tgaattagcc atcggtattt tggatggttt attagaccgt ggcgtgaaag taccggaaga
     2161 attcgaaatc atcacaagta acaattcact actgacagaa gtagctcgtc ctcgtttaac
     2221 aagtatcaca caaccattat acgatttagg ggcggtatct atgcgcctat tgacgaaaat
     2281 gatgaataaa gaagaagtcg aagaaaaaac gattatcttg ccttatggaa tcgaagaaaa
     2341 aggatctaca aaataataaa aggacaacca ttcagaggtt gtgtgcctgc cgtttaactc
     2401 tgcgttacaa ggagcaattt caaaaaacag catctcatgt ttgatgaata ttgtgactcg
     2461 tgattcggag ttggcaggag aacaccgttt atttgggaaa aggggttatg acaaaatttt
     2521 gttataatcc tttttttgtg tcttaaaaaa gagagaaagt ggttgaaatt acatttatcg
     2581 tatataaaac aatccgtgta tagatgctgc attttgttca ccaccatcaa aaaaataagg
     2641 gactgtgaca atactattgt cacaatccct tattactgtg tattctatta aatcaatcat
     2701 ctttctcatc tgattcttta gttgcttgat tcgctcttgc ataagctgaa gcggtgttcc
     2761 agtaatctgt atagttttca tcggtaccgc caacgccaaa tttaaaggcg cttttcttcg
     2821 gtccattttt cgcccatagc gattcaactg tttttcctgg tgtttggatg gattttttat
     2881 tgacagtaac ttttccaggt gtttgaccag taaatccaga tacttttttc ttcatcacat
     2941 cagatgataa ttcgaatttc tcatcagtgc caaaaatgtc aggttttgct tggtaagctt
     3001 gattgatcag attggcaaga taattagccg aacgtatcct tgctttgctg tccattccag
     3061 tattgtcatc gtgccctgcc cagctactga tggtaataga cggagtagag acaattagcc
     3121 atgaatctcg gtattcattg gtagaaccag tttttccgac ccaatctgct tttcctagat
     3181 ttccgtttaa gcttgagatg gcatctttga atggtgtagt gatttttgca ttgatcacac
     3241 tacgcatcat gtcattcatg atcgatgccg ttgcttccga atagatccgg acagagttgc
     3301 tttcgtgttt ataaattaca tttcctgcgt tatctgtgat ggaatcgatg atatagcctt
     3361 cttcatagac gccgccgttt gctaaagcct gaaatccgtt ggtctgttga agcgtcgtga
     3421 catctactgt acctaaagga gcagattcaa cgccccaatt gtcatttgcc gggtagttca
     3481 tctttgccaa gtagtgctcg taagcatatt gtttcgatcc accggaattc aacacatctt
     3541 gatataagtg ataagccggg atattgttgg accattctag tgactcacgg acggtttgga
     3601 aggtgttcga tccttcgtta gtagcattga cgattttctc gccagcattt tctccttctt
     3661 gccaagttgt tgcataatct gatacacggg attctgatcc gataagcccc atgtcgattg
     3721 caggtccata aacaagaact ggtttgattg atgagccagc ttgacgttct gtatcgaagg
     3781 catggttatt ttgattctga ctgtaattcc gtccgccaac gaatccgtag atgcggccag
     3841 ttctgttatc catcaataca tttccggttt ctacttgact gccagcacca tcatctaaca
     3901 tgtatccata attttcaacg ccattttgca atgcagcata gacatcttta tcgattgtcg
     3961 aatggattgt atatccttta ttttggatcg tttgctgtgc cttttgataa taagcgtcac
     4021 tgaccgatga atcagacata tcagcgttgt ctttttcagc caattgttgt gcaatgattc
     4081 ttgttgcttc attcatcaca gtataataca agaattctct gtcagtctgt tctgcgattt
     4141 gttgtggtag aaagtctttt gtaaggtcat aggcttttgc ttcttcgtat tgttctttcg
     4201 taatttggcc ttcacgatac atactgaata aaacaaaatc ttttctggca aggccggcag
     4261 aaagatcttc ttttagcgcg cctgtgttcg tgtatggact atacgtgatc gggctttggg
     4321 gtaatccagc gatataagca gcttgcggca gcgtcacatc ttttgcatct acaccaaaga
     4381 ttccttttgc agcttcctgg actccagcaa tgttttgtcc cttattatta cgaccaaatg
     4441 gtgagacatt caaatacgta gcaatgatct catcttttga aaagtatttt tctacttgtg
     4501 cagaaagaat gatctcgttt gctttacgtt tgaatgttgt ttcatctgta aggatctgct
     4561 gtttgactaa ttgctgtgtc aaagttgatc caccagaaga gccaatacca gtagcttcag
     4621 atactaatgc tcgtaaaaca gctttaggaa cgtaaccttg gtgtttatcg aagtattcat
     4681 cctctgtgct gataattgca gttttcaata aaggtgaaat cttatcagat gaaatagtcg
     4741 tgcgcatcag atctgtctgg atttttgata tctctgtatt atctgcataa acaatctttg
     4801 acgtttctgt aatgtttcct agttctgtct gcagtgcttt tttagtcggc aattgtgtgt
     4861 cttcgacgag ataggcaaaa tagccagcgc ctattccaat acctagagct gcgaacatca
     4921 aaagaagaac agtaataaaa acagtcaatg attgaaaaac tctaaaaata atcttaggaa
     4981 caaaccattt ttcttttttt cttctattct gtttttttcg agtatttttg cgtggcaaaa
     5041 gggtcacctc aactaatggt tttatttcgc taattatagc aaataataga acaaaaaaac
     5101 agacaaggct gtttttggca ggaatttctt ttttaaaaga gttaaaacgt gagaaaatga
     5161 tctttattat aaaagagttc ttaagctttt ctttagagaa aatttcagtt actatggaga
     5221 acagggcaaa gaaaaatcaa tcattttctt tgtatatcat cttaccaata tataggaatt
     5281 gttagtaaca attgcctttc aaggaggaat ccatccatgt tattaggctc acacgtcagc
     5341 atgagcggaa agaaaatgct gttaggagca gcagcagaag cagccagtta tggatcttcg
     5401 acttttatga tctatacagg cgcccctcaa aacactagaa gaaaagccat cgaagagatg
     5461 aatattgatg ccggaaaagc gtacatggca gaaaacggtc taacaaatat cgtcgttcat
     5521 gccccttata tcattaattt ggggaatacg atcaaaccag aaatgtttcc ttttgccgtc
     5581 caatttctga gagaagagat tgttcgtgcg gaagctttag gcgctactca aattacactt
     5641 cacccaggtg ctcatgtagg ggcaggaaca gaagccggaa ttgctagaat tattgaaggc
     5701 ttgaatgaag ttttgacaaa agaacagacg gcacaaatcg ctttggagac aatggcaggt
     5761 aaaggtacgg aaatcggtcg aacatttgag gaattagcgc aaatcatcga tggagtaaaa
     5821 ttaaatgaaa aattatccgt tacttttgac acctgtcata cgaatgatgc cggttataat
     5881 attaaagaag acttttcagg tgtaatggaa gagtttgata agatcatcgg gttagatcgg
     5941 cttaaagtac tccatatcaa tgattctaaa aatccgcaag gttcgcataa agaccggcac
     6001 gccaatattg gttttggaac aatcggattc gacacgctga ataagatcgt ccatgatcca
     6061 atgtttgaca atatttcgaa aatcttggaa actccctatg tcggcaaaga taaaaagaaa
     6121 gcttacgcac catatggaaa agaaatcgcc atgttcaaag cacaaatatt cgatccagaa
     6181 gtgtttcctg agttattagc agaagaagtg atactctaag attttctatc tgacagcagc
     6241 aaaaaaggac caggcacatt gccttgtcct tttttgttta agaaacaaag gagtactttt
     6301 tgcagtcttt ttcatcatgc atcttctccc ccctcttttt aaatttagac aaacaaggta
     6361 tactgttaga aaagtaaaaa aacgaaaagt ttcacaagca agtggcaaaa cgtgattgga
     6421 aggacgaaaa gatgctgaac actttaaaac ccttgatcaa ccaactaaaa aagaaacata
     6481 catactttat tcttggcata gccctattat ttacaattat tcgtttgtat ttatatagta
     6541 aagcaatcta tggaacagac atgtcggcaa cctatgatga ccagctgctg attcgctatg
     6601 ctcaaaatat tttggacggt aaatggctag gggagtatac gactacaaca ctttccaaag
     6661 gaatctccta cagcttattt ttagtacttg cacataaact gcatctttct tatgctgttt
     6721 tgttcggcat gttcagtatt tttgccagta tattggttgc actcgcgttt aaaccgatcg
     6781 tgaagaacaa gtatcttctt ttaagtatct atttttattt tttgttttct ccgattagtt
     6841 ttacacatga atattccact cgaacatatc gtaatgccct cgtgattccg gctgttgaga
     6901 tcattatcgc ttgtttacta gctatttatt accgcaaata cggttctagc agaaaattgc
     6961 tgccatggtt tttagggctt tctgtcgctt ttcctttctt ttggttcatt cgcgaagatt
     7021 ctatctggct tcttcctttt actgtagtag ctttgggtat cagtttcgtc cagatcttgc
     7081 tagggcaaga cttttctttc aaagaagtaa agaagttaaa aaaacgactc tttcttctct
     7141 cgaaacggga atggctgaaa ctttcattgt ttttacttcc tatagttggc ttttccatcg
     7201 tctatcagtg gatcgaatat aaaaatgaga caacatacgg tattgcaacc atcaatgacc
     7261 gttccagtgg agaatttgga cgtttgatga aacaactgat ccgaatagat gatgaaacga
     7321 acttgaataa agaaaacagt gagatatggg tctcacatgc tgcactaaat aaagcattag
     7381 ccgcatctcc tacattccat actgtttcta cccgtattga cgaattatat acgactcacg
     7441 cgtggactca aggtggaaaa gtaaaagaat tacccggaga tatcatcttt tgggcattgc
     7501 gagatgtggt aagcgaatct ggttattacc aaaacaatgc aaaagaaacc aatgattttt
     7561 ggaaaaaagt caaccaagaa ttagaagaag cctatcacaa gggaaccatc aaaaagaaaa
     7621 aagagttcta tctgatggga tcaggagatg gaaagacgat gagtgatctg ccaatcgtat
     7681 tcgatttcct tcaatcagga tacgcttatg catggcgata taaagatttc catcaaggag
     7741 gaaattatag tttcggaacg caagaacaag tcaaacaggc tcaagaatta ttaaaccttc
     7801 cgctaatgga gaattggatc gatagtaatg aaccgagaac acctaatttc caattaacaa
     7861 aatcagcaaa agtcgcgaat gtcatcataa aaatttacca agttctggct attcccgtgc
     7921 tgattgcttc aatagcaggg atattactta tgatactagg aaccgtattt gcaaaagctg
     7981 atcgtgccta ttatagttcc tctttgattg tggtcaccgg attaatactg acacaattgg
     8041 tttttctttt cggtgtctcg tggttttgtt cttatgcgcc agaaaaaaag gattattttt
     8101 taagtgtcta cactggcgca ggcgtgccat taacgcaatt catcgtagta acaagcatgc
     8161 tagttttcag taaattaaat ctgaaaaagg ctttatcttc taaataaaaa gacccttggt
     8221 agctagattt ttaacctaac tatcaagggt ctttgtgtgg aaaaatcagt ttctattcga
     8281 tttcttctac cgtttcctgc aaacgctgtt catatttttt gtttccttta tataaatcac
     8341 caatcgtcca tgccaacagg agaaggacaa gaacaatgat tacataagcg atgtgatcag
     8401 ccaatgctag ttgagcagaa gtggcttgtt caccaaagaa actttcgatc tgtcctggta
     8461 atcctcgtaa gttcaagtaa gttagactaa tcacagagaa ccagccaaga agttttacga
     8521 tgaatgaatt tttaaatcgg ctacccatct ccgcttggct gttggtcatc atcagtaaag
     8581 ggatcatcga aaacggcaac gcaaaagcca agaacacctg ggattcattc atcaagttat
     8641 tcaaagcggt atgttcttcg atcgttcctt ttgtacttgt aaacaaaaca caaatcaata
     8701 caggaatcac agaaagcaat cgtgtgacca gtcttcttag ccaaatcggg atacgcatat
     8761 ggatgaagcc ttccatgatg acttgaccgg tcaacgtacc agtaatagta gagttctggc
     8821 cagaagcaag aagtgctaca gcaaataaag tggataagat accagatttt gctacagcaa
     8881 tcaagatacc attgctcata gctgatggat cagataaggc ttcatacaga ccaaaaaagg
     8941 aaggatcttg gacagaaccg gttttgaaga cagcgacacc catgatcaat agcagtgcat
     9001 tgactagaaa agccatcgac aattggatat tagagtccca agtagaaaaa cgtacagctt
     9061 gtgctacatc ttcttcgtct tggtgatcaa tcttccgagt ttgagaaact gctgaatgaa
     9121 gatataagtt atgcggcatg actgttgcac caataatccc taaagctcca gtcagcggag
     9181 tttcgccgcc gatgaccggt gagtctgaga atgtatgact gttagggata aagccagcaa
     9241 ataaactgtg ccaatctggg ttagacaagc caacttgata gacaaaaacg aataaaatga
     9301 cgaaaattaa gcagacaacc agtgcttcga tcttacgaaa cccgactttt gtcagtagta
     9361 agagaacgaa aacatctaat acagtaataa agacggcaat caccaatgga atatcaaaaa
     9421 gtaagtagag ggcgattgct gcaccaatga cttctgcgat gtctgttgcc ataatggcta
     9481 attcagtcaa gatccataaa atgacaccca atgtcttact tgtgcgtgct cggattgctt
     9541 gggccaaatc catttgggtc acgattccta attttgctgc catatactgt aaaagcatag
     9601 caatcagact agacatcaaa ataactgaca tcaataaata ttggaaattt tgtccaccag
     9661 tgatcgaagt ggaccagtta cctggatcca tataaccaac tgctactaat gcacctggtc
     9721 cagagtaagc aagaagtgtt ttaaaaaaac ttttcccctt tggtacatca actgttccat
     9781 ttacttcagc taaagataaa ccatttgtat gttcaataag tcgatgctta tgaaattgtt
     9841 tgttggattg attattcaag aaaaaaccac ctttcctatt tatctacgat tacattctac
     9901 acctcaaaaa gaaaaaatta aagaaaaata ttcggtatgc ctaatttatt ttcgtggcgc
     9961 gtcttgttag atgacgaaag aaattttgtt ctcctctaaa aaaaccacta ttttttgcat
    10021 gaaactaaag aatacgcctt tcttttttca ataaacttta gtaaaataag tcattgagag
    10081 caattcggaa atagaaagga gagatttata tgttatcaac aggtattgtc gtacttattg
    10141 ctattattgc tttgctaatc ggcgcagtag gtggcttttt cctagcgcgt aaatatatga
    10201 aagactattt agaaaaaaat cctcccgtta atgaggacat gttgcgatca atgatgatgt
    10261 caatgggaca aaaaccatct gagaagaaaa ttcgccagat gatgcagcaa atgaagaacc
    10321 aagggaaaaa ataggttctg acccaacgac tttttacggg ctgatttttt cagtccgttt
    10381 tttattttgt aatttgttag actattcaga ttattcagca aaaagacaag cacagacaat
    10441 cctaggctct tgataaagac aaaggaggag taaaatgtcg atttttagaa agttaggctg
    10501 gttttttcgt caggaaaaga aacattattt aattggggtt ttctcgctat tcgcagtggc
    10561 cttggtacag ttagttcctc caaaagtgat tgggatcatc attgatgaga ttgccgataa
    10621 aaatttatca gtaaagatca ttttcgggtg gatcttccta ttgattatcg ctgctttctt
    10681 acaatatctt tttcgttata tctggcggac gaatatttgg ggaagtgcag ctcggttgga
    10741 gaaagaactt cggcagcagt tattcaaaca ttttacaaaa atggacaatc tcttttacca
    10801 aaaatatcgg actggggatt tgatggctca tgcaacgaat gatttgaatg cgatccagaa
    10861 tgtagcagga gctggtatct tgacctttgc agattcagtg attacgggcg gaacaacgat
    10921 cattgtgatg attttgttta ttgattggcg cttaacactg attgcgctga ttcctctgcc
    10981 tttgctggcc gtcacttcac gagtgctggg gtcgaaactc catgatgctt ttcgtgattc
    11041 gcaagaagct ttttctagta tcaatgataa gacacaagaa agtatcactg ggatcaaagt
    11101 cattaaaaca tttggacaag aaaaagaaga tctagcggat tttgaagaaa agattgaaga
    11161 tgcaattatt aaaaacaaac gagtcaattt tctagactcc ttatttgatc cattcattac
    11221 cttgattatc ggtctgtctt atgttgcaac aattatttta ggtggaaagt atgtcttgga
    11281 aggcacgatc tcgatcgggc agatggtctc atttattagt tatatcggta tgcttgtttg
    11341 gccgatgttt gcgattggtc gattgttcaa cgtattggaa agaggaaatg cttcttatga
    11401 tcgcgtcaat gaacttttgc atgaaaaaac gcatatcgtg gaacaactgg gtgcagtgca
    11461 gaccccggta aaaggcgagc tgaatgtagc tatttcctct tttaattatc cgaaagaaga
    11521 atcaccagca ttagaacaaa tcaagtttgc aatccaagaa ggagaaacat taggtattgt
    11581 tgggaaaact ggtgcgggaa aaacaacgat catcaaatta ctgatgcgtg aatacgatga
    11641 ttatcaaggg aaaatccaat ttggcggacg ttcgatcaaa gaatattcat tgaatgcttt
    11701 attaggatca atcggctatg tgcctcagga tcacttcttg ttttcgatga ccattcgaga
    11761 taatatccgt ttttctaatc caaaactttc agaagaagaa gtagagcatg cggcttcttt
    11821 agcatttatc aacaatgaaa tcaaggaatt accaaaaggt tatgatacct tagttggaga
    11881 acgtggagtc tctttatctg gcggacagaa acaaaggatt tcgattgctc gagcattgat
    11941 caaaaatcct gaattattaa tattagatga tgcactttct gcagtagatg ccaaaacaga
    12001 agaagctatc ttgcataatt taaaagaaat aagaaaagag aaaacaacta tcattgcagc
    12061 tcaccgttta agtagcgtga tgcatgcaaa agaaatcatt gttgtcgatc aagggaaaat
    12121 cattgaacga ggaacccatc atgagctgct tgcactaggc ggctggtatg aacaaatgtg
    12181 ggagaaacag caattagaag cgaagataga aggaggcgag gcctgatgga tgaaaattat
    12241 caatcggaat ggacaaaatc gattccactt aaagaacaga aaaagatcat caaacgactg
    12301 atgaagttcg ccaaaccttt tagaacaacg ttcatcgttg ctatcttatt tgcgtttgct
    12361 ctgtctgtga tcaatgtcct attgccaagg atcatccaaa cttttatgga tgatcatctg
    12421 gctaaacagt cggctacgac acaagttatt ttgttttttg ctggtatgta cttatttgga
    12481 gtcctagtaa aaagtatcat ttggtttttc caatggtatt tgtattccat ggcttcattg
    12541 aagacctatc agtatattag ggtaaaatta tttgaaaagc tgcatacact aggtatgcgt
    12601 tattttgatc agacaccggc aggatcaacc gtttctcgag tgacgaatga cacagaaacg
    12661 ctatttgaat tttggtacgt ctttttgatg gtattgactg ggatctttgc agtcgtttct
    12721 tcattcattg ccatgtttca aatcaatgga gaaattgcct tgtggacttt ggtgttcttg
    12781 ccgattttag gaattgtcat ctggtattac cagaaattta gttctcgtat ttaccgaaat
    12841 atgcgggaaa aattaagtca gctgaataca aaattgaatg aatcgatttc cggcatgcgt
    12901 attatccagc agtttcgcca agaagctcgt ttagctaaag aatttgaaca agtgaacaat
    12961 gagtatttag acacgcgcta tgcgatgatc aaaacgaatt cgattctgtt aagtccgatc
    13021 atcaattttc tatatgcatt ggcgattgcg ttggcgttga ctttatttgg gatcgatgcg
    13081 ttgcattctc ccgtagaagt tggactgatc tatgcattta cgacttatgt ccagggattt
    13141 tttaatccaa tgtctcaaat gatggacttt ttgagcatct tcacagatgg gatggttgct
    13201 ggtagtcgta tcttgaagat tatggatacg gaagaatatg caccacaaca aaaccctgga
    13261 gcagatcaaa tgattacttc aggtaagatt gagtttcgca atgtaagctt ttcttatgat
    13321 ggagagcaca atgtattaaa caatatctct tttgttgcta atccgggaga aaccgttgca
    13381 ttagtgggac acaccggaag tgggaaaagc tcgatcatca atgtgttgat gcggttttac
    13441 gaattttatg aaggtcagat attgattgat gatcatgaca ttcgagaata tccaatggaa
    13501 gagttacgta agaaaatggg gctggtcttg caagatgctt tcttgtttta tggggatatc
    13561 gcaggaaata ttcggatgat gaatccagct atcacagacg aacagatacg ccatgcggct
    13621 gagtttgttc aagcaaacaa attcatcgat gaattaccaa aaggatatca ctcaaaagtg
    13681 atcgaacatg gtgccagcta ttctagtgga caacgacagc tgatcacttt tgcgcgaaca
    13741 atcgtgactg atcctaaaat tctagtattg gatgaagcaa cagcaaatat cgatactgaa
    13801 acagaaggat tgatccaaga aggattggcg aaaatgcgac aaggacggac aacgattgcg
    13861 attgcccatc ggctgtcaac gattcgagat gcagaattga ttttagtttt agacaaaggt
    13921 catatagtag aacgcgggaa tcatgaaaca cttctagcgc aaaatggttt gtatgcagat
    13981 atgtacaaac tgcaaagtag tggtatataa aagatgttgt gacagtagca ttttgaccta
    14041 agcactagag tgaaaataaa acatagcttc ccatattttt catctgtgag agttagtgtc
    14101 gtagttacca gatttaaaaa agacgacgtc agctagtcag aagtgcctaa tagcccctaa
    14161 gaaagcgttg acaaaatcgt ttgctttcga taatataaag aaaacgatac aacttggata
    14221 gtgattgcct gatgcaagct aaacctgatg agtctcccat ggtttcatgg acggcttttt
    14281 gggtttttct ttttgttttg gatacagccc tatatatcat ggaaaggagc aaaacatgct
    14341 gattgagaaa gtactgaata acaacgtcgt aataacaagg gatgaaagag atcgggaaat
    14401 gattgtgatg ggaaaaggat tggcattcaa aaagaaggtg ggtgacccga ttcttgactc
    14461 atgcatcgac aaaaaatttc agttgacaaa tgacggactt tcacaacagt tccaagaatt
    14521 ggcacaagcg attcctcttg agtacttaga aatttcttgt gaaatcattg aatatacaaa
    14581 agaggctctg caagtccatt taaatgattc tgtgtatatt tcattgacgg atcacttgta
    14641 tacagctatc gaacgtcaaa aaatggggat ctttgtgcca aatgtattgc tgtatgatat
    14701 caaacaactg tttcctaaag agtatgccat tgggaaaaaa acagtgaaga aaatctcgga
    14761 taaataccac atcaagttat cagaagacga agccggattt atcgcattgc atttagtcaa
    14821 tgcacaaggc attcaaagcc agcaagacaa tacctacctg tttaccacaa caatacagga
    14881 tattttaaat atcgtccgct attactataa gattacattt gatgaagatt cagtccactt
    14941 ttttcgattc gtcacacacc tgcgtttttt tctgacacgt gtcctagatg gagcgaaatc
    15001 agaagatgga gaagtttctg aggaattgtt ccaagttatt caaaaaaaat ataaggatac
    15061 gtttggttgc gttgagaaaa tagcagactt tttaaccggg gctttcgact accagatgtc
    15121 cacggatgaa aaaatgtatt taacgatcca tactgcgcgt ttagtgcatg agaatcgttg
    15181 taaataaata aaaaaggagg acaaattaac aagaacgtat gaaataaggg tagtgacatt
    15241 cagttggcta aaccttccac taattaattg gagggaatag aaatgggaaa ataccaggaa
    15301 ttagcagaaa acatcgtaaa gaatatcgga ggaaaagaaa atatccgtag cttgacacac
    15361 tgtgtgacgc gacttcgttt caaactaaag gaggaaaata aagcaaagga tgatgttttg
    15421 aaaaagatgg aagggatagt caccatcatg aaaagcggcg ggcaatatca agttgtaatc
    15481 gggaatcatg tgccagatgt ctatgcagaa gtactgacag tagccgggat ttccgatgaa
    15541 gcagaagaag aggtttcttc aagtagtaat ctgttcaatc gattaatcga catattaagt
    15601 ggctgtttcc agcctttttt aggtgctttg gctgcagcgg gaatggtcaa aggattaaat
    15661 gctttgttct tgtttctcaa attttatact gaaggatcgg gtacgtacat catgctgaat
    15721 gggatagggg atgcaatttt ttatttcatg ccagtgatct tagggtacac agcagcgaaa
    15781 aaatttcatc tgaatccgat ggtggggctc gtgattggtg cagcactttg ttatccgact
    15841 gtacaaggag cagcactaca aagtggtttc cctgatggca gtcctgcgcc ttatagcttg
    15901 ctgggtcttc cggcttatga aacatttttg ggtatcccat gggtgggcag cagttatact
    15961 agtagcgtcg tacctgttat cttcattatc gcatttgccg gacaagtcca aaaaattgcc
    16021 aagaaatgga ttcctgcagt cgtccagacc tttttggttt ctttttctgt gttattaatc
    16081 gcacttcctg ctggcttttt gattatcggt ccgattgtca gtatactgac agatctatta
    16141 agcacgggat tccaagcatt gatggacttt tctccagctg tgtatggtgc agttctaggg
    16201 tttctttggc aagtactggt gattttcgga cttcattgga gtattatccc tcttgcgatt
    16261 atgcagctag cgcaaaatgg gtggagtcag atgttagtag gcgtttatgg cgcgagtttt
    16321 gcacagacag cagtggtatt ggcgatgtat ttcaagttca aggatcaaaa aatgaaggcg
    16381 ctttgcatgc cagctgtgat ttcaggaatc ttcggcgtaa cagaaccggc tatttatgga
    16441 atcaccttac cgaagaagaa gccatttatt tactcgatga ttggtggtgc tgtttcagga
    16501 atctatttga tggcatctgg aacaatcagc tacataatgg gcggattagg gatttttggg
    16561 gtggtaaact acatcacgga aacagacgcc agtggtatgt ttcaagcgtt tatcggcatt
    16621 gctattgctt cggtaatcgg gttttcgctg acttttttct tttggaagga tgaagcaggt
    16681 gaaacagatg ctatacagaa aaaatcaggt aaagaaattg taaaaacacc gatacaagga
    16741 caaatcgctc cactaaatgc agcgaaggat gcagcttttg cacaaggaac actaggtcga
    16801 ggaatcttaa tctaccctga aaaaggagaa gtacgtgccc cttttgacgg aacagttatg
    16861 accctgttcc cgacgaaaca tgcaatcgga atggtttctg aaacagggct ggaattgctg
    16921 attcatgtcg gattggatac tgtgcagttg gaagggaaat attttgaaag cttagtgcag
    16981 caaggagaaa gagtgagaca aggcgatctg ttgctacgct tcgacaaaaa gaaaatcgaa
    17041 aaggaaggat acagtttaga aacacctgtg atcgtcacca attacacgga ttatttggat
    17101 attttggaaa accaagggga gacggtcggg ccagacgatg cacttatcac cgcactgacc
    17161 tgaacggtta ggtgaagaat ggactaggct taactattct ttttattcca cttctttatt
    17221 ttcaaaattg aaaggctctt tcatgatggc tgagctgata gggcgtttat cgaatttttg
    17281 accgacagaa attagatgga ctaaattctg ttgataattt tctgcaaaaa cacaactata
    17341 taacacgtct agaagataac aaattgatgt attgatcgta aaattaccaa tttttgaata
    17401 cagtcgctcg cgtgtcgtaa gtcgcaaagc ggctgtactc attgcagata gcgttgaatc
    17461 accgatactg gtcaaagcta aaattggaac atcattttgt ttcaatattt ctgcggtacg
    17521 aaggatcaca ttgttttcac ccgtataaga aatcagaatc ccgcacatat cgctcgtaca
    17581 attgtacgct tcatagaatg attcaccaaa agtggtgaca attgtgacat ccattttgat
    17641 gcgcttcatt ttcagcatga aatcttgaga aatcaaagta ttcgagttac tggcgaagat
    17701 cacgattttt tttgcctgga gcagtaaata tttagctttt tgcagttggt catgatgaat
    17761 gagagaaagt gtatcatgaa tcgtcatctg ttctaaagaa gccattttat ttgcgattgt
    17821 cataataccg tctgtggtgg aaaaaggaag attcgcatct acgtcttgaa aattcccttc
    17881 aagataggct gtttcagcta aaaaagcatt acgaaaactg acccagccat caaaacccat
    17941 tttttttgct acacggatga gtgtagacgg gtggacataa catcgctcag ctatcgcttg
    18001 gatggtcatt tcgccaagtg ctggagaatc gcttaagaga aactggatca cttcattttc
    18061 tgaaggagaa aaggagattt ctttgatttt ttcacgtaat aacatgtaga taccttcttc
    18121 ttattttttt acgtaaatta taactttttt gaaaatgttt tgtaaacgga tttatactat
    18181 ataacttaaa ataggctttt tttgacaaga tatttacaaa aatccactag aaaacagtaa
    18241 cttttgtaaa tgttactgga aagaaacgga tttctattgt ttgttttgtt tacaaaaaag
    18301 ataatgaaag tgtaaaaaag taaaggagtg agaacatggc aaaaggaatc aaaatcgcta
    18361 cgattggtgg aggcagcagt tacacccctg agttgatgga aggatttatc aaaagatatg
    18421 aggaattacc gatacgagaa atctggctcg tagatgtaga agaaggaaaa gaaaagcttg
    18481 agatcgttag tcagatgtct cagcgtatgt gggatgcttc tccatatgaa gtcaagatct
    18541 atgcaactct ggatcgggaa gaagcattga aagatgcgga ttttgttacg actcagtttc
    18601 gtgtcggtca attagatgca cgaatcaaag acgagcggat ccctagttat tatgggatga
    18661 ttgggcaaga aacaaatggt gctggcggga tattcaaagc ttttcgtacc gttccagtcg
    18721 ttttggcaat cgttgaggac atgaagcgtt tatgtccaaa tgcttggctg atcaatttcg
    18781 cgaacccaag tgggatggtg acagaagcag ttatcagata tggcaaatgg gataaagtga
    18841 ttggattatg caatgtgcca gtgatggcga tgatgacaga accagcaatg cttggcaaga
    18901 aaaaagaaga gctgatctac aagtttgccg gtctaaatca tttccattgg cacaaagtag
    18961 cagataataa aggaaatgac cgcacggcag aattgattga tcgtttatac gatgaagata
    19021 atggattacc gaaaaatatc tttgatatcc cgtttttcaa agaacaatta aaacaaatga
    19081 acatgatccc ttgtggctac caccgctatt actatcgtga agaagaaatg ctggaacatg
    19141 ctttagaaga atatcaaacg attggtacaa gagcgcagca agtgaaagaa acagaagcag
    19201 agctatttga attatacaaa gatccagaat tagaccataa acctgaacaa ttgcaaaaac
    19261 gaggtggcgc ttattattca gacgctgcct gtgaaacgat tgcttcaatc tatggaaata
    19321 aagaaacaca gatcgttgta tctacaaaga atgatggagc tgttcctgac ctgtcggcag
    19381 attgtgttgt agaagtcact gcctatgttg gcgcacaagg agcgagaaat gtagcgtttg
    19441 gttctcttcc tgctgctgaa agaggctggt tgcaagtaat gaagaatatg gaattgctga
    19501 cgatcgaagc agctgtgact ggtgattatg ggacagcttt acaggcgttt acgatcaatc
    19561 cacttatacg atcaggaaag acagcaaaac ggatcatgga tgagttgttt attgcacacc
    19621 agcagtattt accgcaattc aaggaaacaa tcagccgatt aaaagaagaa ggaatcgaag
    19681 ttcaagatga actggcaaga gaattatcag gtaatacaca tataatggag gaagtataat
    19741 ggggttcaga aaagattttt tatggggcgg tgctacagca gccaatcaat gtgagggcgc
    19801 ttatgatgaa ggtggacgcg gactagcaaa cgtagatgtt gtgccaatcg gaaaagaccg
    19861 ttttcctgtt atcaccggaa agaaaaaaat gtttgaattt gatgaggatc atttttatcc
    19921 agcaaaaatt gcaatcgata tgtatcatcg ttataaagaa gatattgctt tgtttgcaga
    19981 aatggggttc aagacttatc gaatgtctat cgcctggagt cgtatttttc caatggggga
    20041 tgagaaagag ccgaatgaag ctggcttgaa attttatgag gatgtcttta aagaatgcca
    20101 aaagcacgga attgagccac ttgtgacgat tacgcatttt gattgtccaa tgcatttgat
    20161 caaagaatat ggtgcgtggc gttccagaga actcgttggt ttttatgaaa atctgtgtcg
    20221 agtcatcttc aatcgctata aaggacttgt caaatattgg ctgacattca atgagatcaa
    20281 tatgatcctt catgcaccat ttatgggcgc agggctgtat tttgaagaag gagaaaatga
    20341 agaacaaatc aaatatcaag cggcacatca tgagttggtt gcaagtgcca tcgcaacgaa
    20401 gattgctcat gaagtagatc cggagaacaa agtaggctgt atgctggcag caggagcata
    20461 ttatccatac acttgcaatc ctgaagatgt ttgggcttct agaaaatcag atcgagaaaa
    20521 ttatttcttc attgatgtcc agtcacgagg agaatatccc gcttatgcat tgaaagaaat
    20581 ggagcgaaat ggcattcaga tcgaaatgga accaggggat gccgcattgc tgaaagaaca
    20641 tactgttgat tttatctctt tttcttatta ttcttcacga gtttcgacta ccgacaacga
    20701 actcttggaa cagactgcag gaaatatctt tgcttctgtc aaaaatccat atctgcaagc
    20761 aagtgaatgg gggtggcaaa ttgatccatt aggcttgaga ataacgatga atgatattta
    20821 tgatcgttat caaaaaccat tatttattgt agaaaacggc ttaggtgcag tcgatacgcc
    20881 agatgaaaat ggttatgtgg aagatgatta ccgaatcgat tacctggcag cgcatatcca
    20941 ggcgatgaag gatgcagtag aactagacgg agtcgatcta ttaggctata cgacttgggg
    21001 atgtatcgat ttagtatcag ctggtaccgg tgagatgaag aaaaggtacg gattcattta
    21061 tgtcgatcgt gataaccaag gaaatggcac attagcccgt tcaaagaaaa aatcattcaa
    21121 ctggtataaa aaagtaattg attcaaatgg agaaaatctt tcgaacaatt gatcaatgat
    21181 ttataaaagt ctattgtttt tcagcggatt tttcgagtat aataaagcta ttcgagaaac
    21241 gcaagcagaa caatagactt tttttcgcat tggctgtaaa gtatgcttac ttgattaagt
    21301 aaaggagaat ctacatatgt atgatgtaat cgttattggc gcaggtcctg ctgggatgac
    21361 tgctgctttg tatgcttcac gttcaaactt atccgtattg atgatcgaac aaggtgcccc
    21421 tggcggccaa atgaacaata cggcagaaat cgaaaattat cctggattcg attcgatcat
    21481 gggccccgat ctagcttata agatgtatga aagtgtctca agatttggga cagaaaatgc
    21541 ttatggaatc gttcaagata tcaaggatca cggcagccat aaagaagtca tctgtgaaga
    21601 taaaagttac gaaggaaaaa cagtcattat cgcaacaggc tgtatccacc gtaaattagg
    21661 cgtacctggc gaggaagaat ttgctggacg aggcgtttct tactgtgcag tctgtgatgg
    21721 tgcgtttttc cgtaacaaac gtctagtagt catcgggggt ggggactcag cagtagaaga
    21781 agccatctat ttgacacaat ttgcttctga agtagtgatc gtccaccgta gagatgaatt
    21841 acgtgcacaa aagatcatcc aagaccgtgc atttgcaaat gaaaaaattt cctttatgtg
    21901 ggatacagtc gttgacgaaa tcgttggaaa cgaaatggta gtgacaggtg tgaaagcacg
    21961 taatgtcaaa accggcaatg aatttgattt agcagcagac ggagtattca tctatgttgg
    22021 actggatcca ttaactgcac cttttagaga agcaggtttg acgaacgaag ctggctggat
    22081 tccaacagac aacgaaatga aaacgaatat gccaggcgtt tttgccatcg gagatgttcg
    22141 tgaaaaagat ttgcgacaaa tcactacagc tgttggtgac ggcggtattg ctggacaaca
    22201 agtgtataaa tacttggagt ctttagaaga aacaacagca taagtttaaa aagagtgaaa
    22261 gaacggtttc aattgttctt ttactctttt ttttcgcgtg aaatccactg aatccgattt
    22321 atgtgaatac ttgcaatcat gcttgataaa agaatccttt cttgatttaa gcgttttttg
    22381 gtcctcatac tttcatatct aataagtgaa tgctatactt attaagaacc atatttcaga
    22441 aagaggtgtt attttcatgt cttgggaaca agtgtatgaa caatgggtac atgaagaaaa
    22501 tttagaagaa aacttaaaaa aacaattaaa tgatctgggt gaagatcctg aaaaacttga
    22561 agatgctttt tatgcaccgt tggaattcgg tactgcaggc atgcggggaa tcttaggacc
    22621 tgggatcaat cgaatgaaca tctatacggt aagacaagca acagaaggac tagcacgttt
    22681 catggataca caaggtgaag aaacaaaacg ccgaggtgtc gctatcgctt atgactcacg
    22741 ccatcagtca ccagaatttg caatggaagc agccaaaaca ctggcacatc acaatatccc
    22801 gtcttatgta ttcgaaagtc tgcgtccaac accagagctt tcttttgcag ttcgtcattt
    22861 gaagacgttt gcgggaatca tggtaaccgc atctcacaac ccagcggctt acaatggata
    22921 taaagtctac ggagaagatg gcggacaaat gcctcctgca gacgcagatg cattgacgaa
    22981 atatgtccgt gaagtcagca atccgctaaa agtcgaagta ttatctgacg aagaagctaa
    23041 acatagcggg ttgatcacga tcatcggaga agaagtggac gctgcttatc tagaagaaat
    23101 caaagcagtg acgattgatc gtgaattggt tgaaacaatg gggaaagatt tgaaacttgt
    23161 gtacacgcca cttcatggaa cagggaaaat gttgggcgaa cgtgcgttga aacaagctgg
    23221 attcgaacaa ttcgtactag ttcccgaaca agctgtggca gatccagatt tcactacggt
    23281 caaatcgcca aatccagaag aacattcagc ttttgaatat gctatacgac ttggagaaaa
    23341 agagggagct gacttgctga ttgcaacaga tcctgatgca gaccgtttag gcgcagcggt
    23401 tcgtttgcca gatggttctt atgaagtatt gactgggaac caaatcggag cattgtttgc
    23461 tcaatatatt ttggaagcac atcaatcaag aggcacttta ccagaaaacg cagcggtatt
    23521 gaaatcaatc gtttccagcg aattgcctac agcaattgcg gaacactacg gtgctactat
    23581 gttcaatgtg ttgacagggt ttaaattcat tgctgaaaaa atccaacaat ttgaagaaga
    23641 ccattcacat acttttatgt ttggattcga agaaagttat ggttatttag ttaaaccttt
    23701 tgttcgcgat aaagacgcga tccaagcatt gcttttatta gcagaagtag cagcttacta
    23761 taaaaaacaa ggcaaaacgt tgtatgacgg cttgcaaacg atttttgaaa cctatggcta
    23821 tttcgcagaa aaaacgattt ctgtgacaat gagcggacaa gaaggttctg cgaaaatctc
    23881 tgctttgatg gaaaaattcc gtaaagaagc accgacagaa tttgccggag caaaagttat
    23941 tcaaacagaa gactttaaag aattgacgag aacagaccgt gaaggaaacg tagaaaaact
    24001 aacaacaccg ccatccgacg tattgaagta tacattggaa gaaaatggct ggatcgctat
    24061 ccgcccttca ggaactgagc cgaaaatcaa gttttatatt ggtgtaaaag gaagctctac
    24121 agaagacgca gccgaaaaag tagcctctta tgaagcgatt atcaaaaaga tgacagaaga
    24181 ataaaaaact tgagactgat ggaaaaagtg gtaaacttta gaagtcagtc ttttttcaag
    24241 ttggaggtga atggattgcc agacacgcaa aaatcaaaag tagagaaagt cagtcaatta
    24301 ttcaaaatgt tgagtgaccc cactcgtctt aaaattctgc tatatctgaa agacggggaa
    24361 caaaatgtga cagcaatcac ccaagcagtg gaaatggagc agtctgctgt atcgcatcaa
    24421 ctacgtttat taagagaaaa tcatgtcgtc aagtctcatc gcgagggaaa agcgattttg
    24481 tatagtcttg atgattctca tgtactggat attttgaacc agacactcaa acatgtggaa
    24541 catcaataac gataggtgca aataga
//
LOCUS       DASJTC010000037        24427 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.037, whole genome shotgun sequence.
ACCESSION   DASJTC010000037 DASJTC010000000
VERSION     DASJTC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.037"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(54..788)
                     /locus_tag="U4B19_001348"
     CDS             complement(54..788)
                     /locus_tag="U4B19_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEN2077097.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="U4B19_001349"
     CDS             1115..1525
                     /locus_tag="U4B19_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEN2077098.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="U4B19_001350"
     CDS             1518..2219
                     /locus_tag="U4B19_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEN2077099.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="U4B19_001351"
     CDS             2219..3805
                     /locus_tag="U4B19_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2077100.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="U4B19_001352"
     CDS             3824..4045
                     /locus_tag="U4B19_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEN2077101.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="U4B19_001353"
     CDS             4042..4803
                     /locus_tag="U4B19_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEN2077102.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYVLGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="U4B19_001354"
     CDS             5183..5692
                     /locus_tag="U4B19_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEN2077103.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="U4B19_001355"
     CDS             complement(5767..6306)
                     /locus_tag="U4B19_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEN2077104.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="U4B19_001356"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="U4B19_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEN2077105.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="U4B19_001357"
     CDS             7319..8170
                     /locus_tag="U4B19_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEN2077106.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="U4B19_001358"
     CDS             8198..8833
                     /locus_tag="U4B19_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEN2077107.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="U4B19_001359"
     CDS             8853..9626
                     /locus_tag="U4B19_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2077108.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="U4B19_001360"
     CDS             10034..10831
                     /locus_tag="U4B19_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEN2077109.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="U4B19_001361"
     CDS             10809..11222
                     /locus_tag="U4B19_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEN2077110.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="U4B19_001362"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="U4B19_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN2077111.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="U4B19_001363"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="U4B19_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEN2077112.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /gene="maf"
                     /locus_tag="U4B19_001364"
     CDS             15999..16559
                     /gene="maf"
                     /locus_tag="U4B19_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEN2077113.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="U4B19_001365"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="U4B19_001365"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEN2077114.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="U4B19_001366"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="U4B19_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEN2077115.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="U4B19_001367"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="U4B19_001367"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEN2077116.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="U4B19_001368"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="U4B19_001368"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEN2077117.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="U4B19_001369"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="U4B19_001369"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEN2077118.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="U4B19_001370"
     CDS             21906..22484
                     /locus_tag="U4B19_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2077119.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="U4B19_001371"
     CDS             22536..23018
                     /locus_tag="U4B19_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEN2077120.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="U4B19_001372"
     CDS             23135..23509
                     /locus_tag="U4B19_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077121.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23939..>24427
                     /locus_tag="U4B19_001373"
     CDS             23939..>24427
                     /locus_tag="U4B19_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HEN2077122.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMD"
BASE COUNT         8007 a         4389 c         5213 g         6818 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgtactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgac
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcaccgggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggta ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatggaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaaca atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tgagagtata aaatattttg tgtaaatgaa aaaatccata caaaaaagga
    23881 agtcccttct gtagaataaa gttaacgaca accaattcac agaaaagagg acttccctat
    23941 gaatgatttt actacagaaa ttgtgcaaac tctagtcact aaaggcgatt taaatgaatt
    24001 attccgttcg cacttagaaa aagcgataaa cacactccta cggactgaat taacggcttt
    24061 tttagattac gaaaaatatg atcgcactgg ttttaattca ggtaattcga gaaacggttc
    24121 ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca ttggaaatac ctagagatcg
    24181 taatggtgag tttaaacaac aaactttacc agcctacaaa agaacaaacg atacattgga
    24241 aaccactatt atccatttat tcgaaaaagg tgttacgatg tctgaaattg ctgatttgat
    24301 cgaaaaaatg tacggtcatc actatactcc acaaaccatg tccaacatga ctaaagttct
    24361 gactgaagaa gtaaatgcct ttaaatccag agccttaaat gataagtatg tcgctatttt
    24421 tatggac
//
LOCUS       DASJTC010000038        24376 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.038, whole genome shotgun sequence.
ACCESSION   DASJTC010000038 DASJTC010000000
VERSION     DASJTC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.038"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(541..1566)
                     /locus_tag="U4B19_001374"
     CDS             complement(541..1566)
                     /locus_tag="U4B19_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2077123.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1841..3526
                     /locus_tag="U4B19_001375"
     CDS             1841..3526
                     /locus_tag="U4B19_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEN2077124.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3540..4823
                     /locus_tag="U4B19_001376"
     CDS             3540..4823
                     /locus_tag="U4B19_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN2077125.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4893..5762
                     /locus_tag="U4B19_001377"
     CDS             4893..5762
                     /locus_tag="U4B19_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2077126.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5759..6595
                     /locus_tag="U4B19_001378"
     CDS             5759..6595
                     /locus_tag="U4B19_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2077127.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6621..7688
                     /locus_tag="U4B19_001379"
     CDS             6621..7688
                     /locus_tag="U4B19_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEN2077128.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7685..8512
                     /locus_tag="U4B19_001380"
     CDS             7685..8512
                     /locus_tag="U4B19_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEN2077129.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8557..9582
                     /locus_tag="U4B19_001381"
     CDS             8557..9582
                     /locus_tag="U4B19_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2077130.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9579..11762
                     /locus_tag="U4B19_001382"
     CDS             9579..11762
                     /locus_tag="U4B19_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEN2077131.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11762..12892
                     /locus_tag="U4B19_001383"
     CDS             11762..12892
                     /locus_tag="U4B19_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN2077132.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAKHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12889..13566
                     /gene="pgmB"
                     /locus_tag="U4B19_001384"
     CDS             12889..13566
                     /gene="pgmB"
                     /locus_tag="U4B19_001384"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEN2077133.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13650..14177
                     /gene="msrA"
                     /locus_tag="U4B19_001385"
     CDS             13650..14177
                     /gene="msrA"
                     /locus_tag="U4B19_001385"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2077134.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14378..15064)
                     /locus_tag="U4B19_001386"
     CDS             complement(14378..15064)
                     /locus_tag="U4B19_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEN2077135.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15144..16682
                     /locus_tag="U4B19_001387"
     CDS             15144..16682
                     /locus_tag="U4B19_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEN2077136.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16802..17269
                     /locus_tag="U4B19_001388"
     CDS             16802..17269
                     /locus_tag="U4B19_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEN2077137.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17788..18231
                     /gene="rplM"
                     /locus_tag="U4B19_001389"
     CDS             17788..18231
                     /gene="rplM"
                     /locus_tag="U4B19_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEN2077138.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18247..18639
                     /gene="rpsI"
                     /locus_tag="U4B19_001390"
     CDS             18247..18639
                     /gene="rpsI"
                     /locus_tag="U4B19_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN2077139.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18727..19953)
                     /locus_tag="U4B19_001391"
     CDS             complement(18727..19953)
                     /locus_tag="U4B19_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2077140.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20025..20183)
                     /locus_tag="U4B19_001392"
     CDS             complement(20025..20183)
                     /locus_tag="U4B19_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2077141.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            20875..21594
                     /locus_tag="U4B19_001393"
     CDS             20875..21594
                     /locus_tag="U4B19_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2077142.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            21596..22438
                     /locus_tag="U4B19_001394"
     CDS             21596..22438
                     /locus_tag="U4B19_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2077143.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            22435..23384
                     /locus_tag="U4B19_001395"
                     /pseudo
     CDS             22435..23384
                     /locus_tag="U4B19_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            23399..23632
                     /locus_tag="U4B19_001396"
     CDS             23399..23632
                     /locus_tag="U4B19_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEN2077144.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            23963..>24376
                     /locus_tag="U4B19_001397"
     CDS             23963..>24376
                     /locus_tag="U4B19_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2077145.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGM"
BASE COUNT         8025 a         4161 c         4990 g         7200 t
ORIGIN      
        1 gtctaaaata atttgtctaa ctagaagaaa agtgggaaag gatcttaaca acatcggttc
       61 gtatccaatt aaggtatcgg aacgatcatc ttccaagaga agtcaatcaa caaaagtaaa
      121 gatagatcat tttttgaatg caaaataaat tgatgggaag atagagcggt tatttttaat
      181 attttgctag ctattattga ataattcctc gaataaaaca aaaccagtag acatgtctgt
      241 tattctattt atgttttaga gataagtatc cccaaataac atggattgaa ataagggtaa
      301 atgtgtttaa aaaaacgaac tataaaaact tatacacaac gtggttaaaa atagtttttt
      361 cttccctgtg gaaaaaatag tttaacacaa acattatagt tatccacagc tatctaaaac
      421 agatggaaaa taagaaaaat tcgctaaaat accgggaggg ggtaacgagg gatgagtgat
      481 tgaaaaaaga acagagtaac tgacttatcc tcagtgtttc tgttctttta taattctgtt
      541 ctacatttct tgttgttgga tcgaatctgt gagagtaatc tcggttgaaa gaatgattct
      601 tttttcttca gggtattggt ggtggattag ttggaataaa agttctgtgg ctttgattcc
      661 taactgattt gtcggaatgt ctatccgagt caactgtggc gaaaataggg aggacaataa
      721 gggatcactg aatcctgcga tcgaaagatc atcaggtatc tgctttccag attgttctgc
      781 cactttgtaa atactcaatg attttaatgc atcggtagaa aaaatcgcag ttggtctttc
      841 tcgtagcttc attaaatcat tcattgctgc catgctatct tctaagcgaa agccgccgtc
      901 tataatccat tcattacgga tatctaggtg gtgatcaaat aagcattgac ggtaaccatt
      961 gagacgatca ttggatacat aaacttctgt cggagcatgg agacagccga tatgacggtg
     1021 tccattcttg atcagtgttt ccaccaaatc gtaactatcc tggaaattat ccgtgtccac
     1081 tgagtagatg ttcgtgtagt cgtttccgac ttttccgatt accacaatag gaatattgta
     1141 ttgatccaat tcaaaaagaa gtgtttcatc catcgtagag cttaaaataa taattcctcg
     1201 gatcattttt tcttggatct ttttcgcgca tttttctagc tcttcttctg gtgtttttgc
     1261 cgtttgtaga atcaagtcga agttattttg ttcagaaacg gcagaaatgg attgaataac
     1321 atctgaaaaa aaaggattgc cataggtcgt atgagaagta cgatgagata atgctagaat
     1381 cgcgtcaaaa ccatttcctg acagtcctct tgcaattttg ctcggttcat aattcaattc
     1441 tttgattgct tgaagtacac ttgcttttgc tttatcagag atatttggct ggttatttaa
     1501 tactctggag acagttgatt ttgagacatt agcttttttc gcaatgtcat aaatagtagg
     1561 tttcaatttt atatgcacct cctttttggg agcgttttag aataattcca tagtacaata
     1621 gacattcttt tttgtctatg attttgaaaa tttaggaata gcttgcatga tcatgagaga
     1681 ccatattttt ttaagaagct attaattatt gatacataag ctttcttatt tgtatttttg
     1741 tgtatatcaa aattatttaa aaaaatgcta ttgacagtaa gcgttttcca taatatgatc
     1801 aaatcgttgg gaacgctccc aaaaaagaaa ggggaatccg atgtatacaa aacttttaca
     1861 agcgatttat cgcgactcag atttgtctga gaaaaccgcc tctatgattg aacaaaaagt
     1921 cagtgtttat cagcagaaga aaggccaggc aggcaaactg cattgggacg aagaagatat
     1981 tttcttgatt acttatgggg atcaattttt tgaagagcag caaccaactt tgcgtacatt
     2041 taagaaattt tatcaaaagt atttggcaga tacatttaga gttgtacatt tcttgccatt
     2101 tttcccatat acatctgatg atggtttctc cgttgtaaat tatcaggcaa tcaacgaccg
     2161 tttgggcaca tgggaagata tagaagaaat gagaagcgaa acacggttaa tgttcgactt
     2221 tgtctgcaat catatgtctg ccgaaagtac ttggtttcag gaatatttaa acttgaatga
     2281 tacgtacaaa gattttttta ttgatttgcc actggataca gatacaagca gcgtgactag
     2341 accaaggacc agcccgttac ttacaacgtt cattgacaaa aacgggaaag aaaaacacgt
     2401 atggacgact tttagcgaag atcaagtaga cttgaatttt aaaaatccag aagttttatt
     2461 gaaaatgatc gatattcttt tgttttactt agaaaaaggc gcttcattta ttcgcttgga
     2521 cgctgtggga tttttatgga aagaactagg aacttcttgt atacacctgc cagaaacgca
     2581 taaaatcatc caattattcc gtgcaatcgc tgatgaagca gctccaggaa cggtattgat
     2641 cactgagaca aatgtgccac acaaagataa tatcagttat tttgggaatg gcgagaatga
     2701 agcacagatg gtctatcagt tttcgctacc acctttgacc cttcaagcga ttcgttcggg
     2761 taatacaagt aagttgaaag aatggttaaa aaccatcgat ttcgcatcga aaaaaacaac
     2821 tttttttaat tttttagcct cccatgacgg tatcggattg aatccggtac gaggtattct
     2881 gccggaaaaa acgatccttg aattagtgga acagctgaag aaagagggta cccgagtaaa
     2941 ctacaagaaa aacccagatg gaacgaccag tccatatgag ataaacgcta cgtatctaga
     3001 tgcgttaagt tctccaacag atacgacgga atggagaagc aaacgctttt tagctgctca
     3061 ttcatttttg gtaacgttac caggcgtgcc ggcaatttat attcaaagtg tgctgggaag
     3121 tcgaaatgat tacaaaggcg tagaaaaaac aggagagaat cgaaaaatca atcggcaaaa
     3181 atttttgtta gcagatattg agaaagagtt gcagacagat ccattaagaa cagcagtata
     3241 tggtgaattg acccagatgc tatctattcg aaaaaaagaa aaagccttcc atccgaatag
     3301 tttgatttac ttactggact cagcagattc ttttatcagt tttattcgcg aagcgaagga
     3361 agagaaagtc ttagttatcc ataatttttc caatcaatca gcaggttatc ttttaccaaa
     3421 aggagattac ctagacctgt tttctggaca aattatccat tgcagtcaag aacgaaagtt
     3481 gatattccag ccgtatgaat atatgtggct gaaaataata aaataagagg tggaaaaaaa
     3541 tgaaaaaaat tattgttact acctgtctgc tagctactgg atttcttctt actgcttgtg
     3601 gtgaaagtca aagctctaac gaatcacaac agacaacgat tcaattcatg cactcttctg
     3661 tcgaacagga acgattaagt gtgatcaatg aactggttgc tgattttgaa aaagaaaatc
     3721 cagacatcaa aatcaagcaa gtgccggtag aagaatcagc gttcaataca aaaatcgtta
     3781 cgttagctcg ttccggtaaa ttaccagaag taatggaagt cagtcaggat tttgctaaag
     3841 tgatggataa agatgaactg attgatcaga cggcagtcca aaacgttatc gaggaagctg
     3901 gagaagataa ttattatgac ggagccaaaa accttgttcg ttcagaagat ggaaaaagct
     3961 atattgctgc accaattagc ggctgggttc aaggtatttg gtatgacaaa gaaaaattat
     4021 cagaagctgg tttttcagaa ccagaaaatt gggatgatat tttaaaaatt gctcaacatt
     4081 tcacagataa agagaataaa caatatggaa ttgctatgcc tactgctgaa ggaacaatga
     4141 gtgagcaagc gttctctcaa tttgctttgt caaatggagc aaatgttttg gatgatgaag
     4201 gcgaggtaac gatcgatacg gaaaagatga gagaagcatt gtcgttttat caaaacttat
     4261 cccaatacac gatgccaggc tctaatgatg tgacagaaat caaagatgct ttcatgaatg
     4321 gaacagcacc aatggcaatc tattcaactt acattttgcc ttctgtttat gaagcgggga
     4381 actcagaaaa tatcggtttt gctatcccaa cacaaaaaga tcaagctgtc tatggcacag
     4441 tatctggatt gacgatttct tctggtctag acgagaaaca aaaagaggct gctgaagcat
     4501 tcacagcttt cttatctcaa ccgaaaaaca tggaaaagtg ggtcttgatg tctcctggtg
     4561 gtgctcaacc agtgaataaa caagtagtcg aactggatgg ttacaaagaa aatgaagtca
     4621 tcaagtcttt tggcgagctg ccatctgaga ttgctagtgc ttttgatgaa gtacaagtct
     4681 ttggactagt cggggaaaag aatttcacga aaatgggaga tatcacaagc tcaggtgtga
     4741 tcggaaaagc agtgaaccaa gtaactgtcg gaaacgaaga tgtcgatcaa gctttagctg
     4801 acgcacaaaa aaatacgaaa taacaagaag ttctgccaat ggtgttcgaa aaaagtagat
     4861 agctattggc agattatcag ggggaggttt caatgaatcg tccaaggaaa cattctgact
     4921 taaaattagc catgggactt ttagcaccaa gcatgctttt attagctgtt ttagtgctga
     4981 ttccaatgct ttccaatatt cagatcagtt ttttcgatat gccgctgaat cctaactcag
     5041 acgccgtttt tatcggatta agcaattatc ttgaaatttt aagtgatagt gaattttatc
     5101 gttctttagg tttaacagtt gcttacacat tcttagttgt agcaggcagt acagctttag
     5161 gattagccgt agctattttg cttaaccgca agttcagatt taggaaaacg gtgcgttcac
     5221 tgattttatt aagttatgtt acgccatcta tttctttgat ttttgcttgg aaatatatgt
     5281 tcaataatag ttatggaatc gtcaatgatc tgttagtaga taggttgcag cttttcaacg
     5341 aagcaccgct gtggttcgac cagccagttg ctagcttctt tatggtcgtt ttgtttgcta
     5401 tctggagata ctatccgtat gcgtttattt catttttggc tattttgcaa acagtcgaca
     5461 aaacgcttta tgaagcagct gaaatggacg gtgcaaatgc ttggcagaag ttcaagatcg
     5521 tgacactgcc ggcaatcaaa ccagtactag caactgtgat tgttttacga tctatctgga
     5581 tgttctatat gtttacagat gtctatctac tgacgaataa agtcaatatc ttaggcgttt
     5641 atttgtatca gacagccttt gcgttcaatg atcttggaaa agcagcagct atatcgattc
     5701 tattgttcat cattattgca gcggtcgtct tgctctcacg aaagaaggtg gacttgaaat
     5761 gaaaaataaa aagagcagga aaatcttatt ttacgcgacg attttgttgc tattagctgt
     5821 caatttgttt ccatttttga tcatgctgat gacatcattc aaggattcac aagaagcaat
     5881 tgcaacgaca caaactattt ttccgaaaga atggactttt gaacattatc aggatatttt
     5941 caatgctgag atttttccat tcatcatgta tttcaaaaat agtttagtga tctccttgat
     6001 agcctccgta actgcagtag tgattgggat cagcggggca tacgctttgt caaaactacg
     6061 cttcaaagct cgctcgaaaa tcaatgctag cttttacacc gtttatatgt tttccggtat
     6121 tttgatcgtt gttccattat ttaaaatcat ttccggtcta ggtttgtatg ataccaatac
     6181 agcactgatt atcacgatgg tcgttcaaac acttcctact gcgatattca tgctaaaaag
     6241 ttattttgac acgattccga ctgatattga agaagcagca atgatcgatg gtcttagccg
     6301 gatccagatc attattcgga tcattatacc attggctatt tctggaatca tttctgtctt
     6361 cgtttattgc tttatggttt cgtggaatga ttatttattt gcttcgattt tcctgtcgac
     6421 ctccgaaaaa tttactttac caatcggttt gaacacatta ttcagtacac cagactacat
     6481 ttggggacga atgatggccg cttcattggt gaccgcttta ccagtcatca ttatgtatgc
     6541 gttatctgaa cgatttatta aaggtgattt agtcgaaggc ggcgtaaaag gataaaaaga
     6601 aaagaaagag gaaagaaaag atgaaaaaat tagttgcatt agaaccaaga atagccggat
     6661 tcaaagatta cgaagaccgt gagatcgaaa agaacgaagt acgtatccga gtaattttcg
     6721 ctactccaaa accaggaaca gaaattgtcg actttagagg attaagtcca tttatcgatg
     6781 aagaattttc agaagaatgg cgcctattct tgccaagaga agaaggaagc caacgcggtg
     6841 tggtatttgg cgaatttcaa ttaggaaata tgattgtcgg tacgatcgaa gaaacgggca
     6901 gtttggtgac agagtatcaa aaaggagagt tagtctgcac ttatggaccc attcgtgaaa
     6961 cgctgattac aaacggtgtt gataattata aactacgtaa acttgatcgg ttggatcaat
     7021 ggaaaaatgc agtatgttac gatcctgctc aattcgcttt aggtggtatc cgggaagcac
     7081 atgttcgagc aggtgatgct gtagcaatct tcggtttagg cgctatcggg caaattgcga
     7141 ttcaattagc taaaaaagca ggtgctacta ccatcgttgg gatcgatccc atagcgcagc
     7201 gtagagaatt agcagaaaaa tacggtgccg attacacgct agatcctatg aaagcagatg
     7261 tcggttttcg tctaaaggaa ttgacagata aacgtggagt agattcagtt attgaaacga
     7321 gtggcagtgc tgccgcatta caagcttctt taagagggat cggctatggc gggatcattt
     7381 cttatgttgc ctttgctaaa ccttttccag ctgggtttaa tcttggtcgc gaagcacatt
     7441 tcaataatgc aaaaatcgtc tttgctcgag catctagtga accaaaccca gattacccaa
     7501 gatggaaccg taaacgaatc gaggacacat gttgggaatt attgatgaac ggttacttgg
     7561 attgtgaaga aatcattgat ccagttgtgc catttgcaga aagcgcagaa gcttacatgc
     7621 gttatgtaga ccagcaccca gaagaaagta tcaaaatggg cgttcaagtc cgaggaggag
     7681 aacaatgaaa ttcggtacgc aagatcaagc atttttccca agagacattt tagaaaaatt
     7741 tagatttatc aaagactgtg gatttgaaga atacgaaatc gacggtaagt tgttaataga
     7801 aaatatcgac acagtcaaac aggcaatcaa agaaacaggg atcccagtag tgacagcttg
     7861 taacggctac gatgggtgga tcggtgactt tatcgaagaa cggcgtctta atggtgtacg
     7921 tcaaattgct gagattttgc gtgcactaag cgaggttgga ggcaaaggaa tcgttatccc
     7981 agcagcttgg ggaatgttca cttatcgatt accgccgatg gtttcacccc gaagcaaaga
     8041 aggagactat aaagcagtca gcgaatcact tcgttattta gataaagtag ccgaagaaac
     8101 aggtacggtt atttatttgg agcctttgaa ccgataccaa gaccatatga tcaatttgtt
     8161 agcggatgcg cgtcattaca ttgactctaa tcaattgaaa catgtgcaga tcatcgctga
     8221 tttttatcat atgaatattg aagaagatga tatttcaaaa gcattgacta cgcaaaaaga
     8281 gtatgtgggt catatccatt tagcagataa tcatcgctac caaccaggat ctggcgcaat
     8341 tgattttact cgccattttc aaacgctact tcaaaatgag tatgaaggtt atatggtata
     8401 tgaatgtcgt ttgcgaggag aaaatgaagt gaaaatttat caggaatcat tgcaatactt
     8461 gaaaaactgt ctggaaacag cgcgtcattt aaatgattta gagaaagaat agagaaagaa
     8521 taacaataag taaataagca aaggagggag aaataaatga agaaagtagc aatcataggc
     8581 gctggacaag tagcagaaaa agtacatgct gcttattatc aaaaaagatc agatgtctta
     8641 gaattggccg ctgtggttga tccagtcagc cagcgagcag aagacttttg ccgacgtaca
     8701 ggattttctc atgcgtatac atcagttaca caaatgctga atgagatttc tcccgatatc
     8761 gtgagtgtct gtacacccaa tcgctttcac ttcgaacatg tattggctgc cctcgaagct
     8821 aatgcttcag tgatgtgcga gaaaccacca gctatgacag ctgatcaagc aagaaagatg
     8881 gcagagacag ccgataaaaa cgggcagatt ttggcttatg gattccagca tcgtttttct
     8941 gacgaagcaa caatgatcaa gaatcatctt gatgttctcg gggatattta ttacgtagaa
     9001 gcgaatgcgc ttcgtcggtc tggtgttcca ggctggggaa cgtttatcga caaagaactt
     9061 caaggaggcg ggcctttgat cgattatggc attcacatgc ttgatacagc gatgtatatt
     9121 actggatttc ctaaagtaaa aagcgttcgg gcgtacagct tccaaaaaat tggtacgaaa
     9181 aaaaattcag ggaaattcgg gaaatgggat ccaagcaaat tcacagtaga agatgcaatt
     9241 tttggcacac tggaattaga aaacggcaag atcatccaac tgaatacttc gtttgctttg
     9301 aatatcagag aagacaaact gaatgtggct ttttgtggcg atcaggcagg tgctaatctg
     9361 tatccaggcg aactattcga agatgtagat ggaaagctgg tttccttaca aaagaccacg
     9421 ccagttaatg acccacatga actaagtatg gctcattttg tgaatcgggc gctcggtgac
     9481 cagactcaga tgattgcaga tggctggcaa ggatatgtca tccagaatct ggtggaaaat
     9541 ttatatcgtt cggcagaaac aggagaagtt attaccttat gaaagtagaa gaaaaacagt
     9601 ttgatttaga gtatatcaat aagtatgcga gtcagatgac cattggaaat ggattttttg
     9661 gtatcagagg aagccaggag gaagactatc ggaatcaagt aaggggaatg tttgcagctg
     9721 gtgtatataa ccgaccttta ggagcagaat cagccgagtt agtgaatctc ccagatgtca
     9781 tcagatggga aataaaaatc gacggtgaac atttttctct tgagacgatg aaagttcttg
     9841 catacgagcg ggcactagat ttagaaatgg gagagcttaa acgctatgcg ctcattgaaa
     9901 ctgctcaagg aaaaaaagtg gaagtcatta cttgtcgtgt tgctgatcaa aataatctcc
     9961 atcaggcagc cctgaaagtg gtcatcaagc cgttgaccga agactgccaa gtagatgtga
    10021 aaacgggtat cgatggacag caaaccaatt ttggtacaca gcagttggtt gaaaaagaac
    10081 tgcgagtatt tggtgacgat ttgatcagtg catcctacca gacaacagaa agtaaaattg
    10141 atattgggat tgcagctaaa ttcaataaag caggtgtatt ccaagcaaaa aatcgccgga
    10201 tcaccgcttc cttttcagaa aagatcagaa aaaatacgtc ctttacgctg gagaagatca
    10261 tcgtgctctc tacctctctt cagcaaacag acccgtttaa gaaaagtatt gcccaagccc
    10321 agaaaagcgt tagttataca gaaattttag agcagtcgat gtcttattgg acagattttt
    10381 ggcatcagca tcggataacg atccaaggcg attcttcttt cgatcagtta gcgatggact
    10441 tcgctgctta ccatttacag attatgactc cccgcaatga tccaagtgtt agtgtagggg
    10501 cgaagggact aactggcgaa ggttataaag ggcatgtttt ctgggatacg gaaattttta
    10561 tcctgccttt ctttttatat cagtttcctg aagtagcaaa gaatttgctg ctgtaccgtt
    10621 atgatcgatt agaaggcgca cggcaaaaag caagagatta tcactatcaa ggtgcacttt
    10681 tcccatggga aagcgcacac actggtcagg aagagacacc caaatttgct gcattgaata
    10741 tcaaaaccgg aacaagacaa gaagttgctt cagctgccgc agaacatcat atcagtgcag
    10801 accttgctta tgccgttcaa gagtatgtcc aagcaacaga ggatcaatcg tttttagaaa
    10861 caaagggaaa aacgctcatt catgaaacag cagctttttg gctgagcaga gctgtaaaag
    10921 tcgatgatca tttagagata cacagagtca ttggtccaga tgaatacaca gaatatatcg
    10981 acaataatgc atatacgaac tatctggcgc attataatgt ggccttagct gttaaatatc
    11041 aagctggcga tccgcttttt ttgaaagaat gtcaaaattt tttagataag ttatatttgc
    11101 cagtgcctaa caaagaacag ctgattccgc aagatgacac gtatctcagt aagccagtta
    11161 ttgatttgac cacttacaaa gaacgtcctg gcagccagtc tattttactt gattatgccc
    11221 gtagcgaagt aaatgaactc caagtcagca agcaagcaga tcttgtcatg ctgttatact
    11281 tattacctga gtatttttct caaccaatcg tttctaagaa tttgcattac tatgaaaaca
    11341 aaacaatcca cgactcatcg ttgagtaaag cgattcacgc catagaggct tgccgtatcc
    11401 aagaaatgga atgggcgtat catttattcc aagaagcgtg cctgattgat ttaggaccag
    11461 atccgcattc atcagatgac ggaattcatg ctgcttcatt aggagcacta tggctagcag
    11521 ttatatttgg tttttcaggt attacagtaa cgaatggaac tctttctatt tctccaagac
    11581 ttccttcagc atgggaaaaa atttcttttc cttttatttg gaaaaatcac aggttgctat
    11641 ttgttttgac aaaaaaacaa atagtaataa aaaaagagac gaaagaaaca ttacctattt
    11701 ttgtcaatgg agaccaatac cagctggaaa aagaactgat cttggaacgg aggggaaaat
    11761 aatgcgtatt ctgattgcgc cagattcttt caaagaaagt ctgacagcct tagaggcagc
    11821 acaagccatt caatcaggtt ttcaagaagt ttttgttcag gctgaatatg atctgctacc
    11881 tataggcgat ggaggagaag gaacggtttc atcattagca gacagtttaa ggttgaagcg
    11941 gcaaacagca gtcgttacta atgcatttgg caaaaacaaa gaaatccagt acgcttcaga
    12001 cggtcaaact gcgatttttg aaatggcaga aatcgtcgga ttgaaagatg tcccactaaa
    12061 ccaacaacag ccacttaggt tatccacgcg aggagtagga gaactgatac gacaattgtt
    12121 gcaacaaggt gttcgacaca tcatcatcgg cgttggcgga agtagtacca acgatggcgg
    12181 catcggcatg gcagaaggtc taggttatgc tttttacgat caagataatc aaaaagtaga
    12241 agcactggga gaaaatctag gtaaaattgc tacagttgat aaaacgaacg tgcctattga
    12301 gctgattaaa gccaaaatca ctgtcattac tgatgttact aatcttttat gcggaaaaga
    12361 aggcgcttct tttgttttcg gaccacaaaa aggcttgaaa gaaaatgaat tagcagtggc
    12421 tgaccaagcc aagcatgatt tttatatgag atttgcccct caagtaatcg aggcatcggg
    12481 cagtagtgct ggtggtggaa tgggtgcagg actgttagca tttgcttcag ggaaaatagt
    12541 ctctggcatc gactacgtat tggatttact ggagttcgat aaacgggtac agcaagcaga
    12601 cctcgtaatc gtaggcgaag gacgtctaga tggacaaagt ctaaaaggaa aagcgccagt
    12661 tgggattgct agaagagtac cagagggcgt tcctgtgata gctatttgcg gaagtgttgg
    12721 taaaggaagc gaaaaagcag cagatgttgg catttcggcc gtttttccga tccttcctgc
    12781 agctgtgcct ttacaagaag ctttacagca agccactgat aatcttcatc gcacagctaa
    12841 aaacatcgca tctttacttc aagtcgttca ttttcaagga ggtaatcgat gaaaatcaaa
    12901 gcagcactat tcgatttaga tggcgttctt gtagacacag ctcgttatca ttacgaagcg
    12961 tggctggttt tagctaacca gctgtccatt ccattcactg agaaagaaaa cgagcaactg
    13021 aaaggaatat ccagaaccga gtcactagag cgattgctgt catttggaaa aatggaacag
    13081 aaattctcag aaaaagagaa atcagccttt gctgagcaaa aaaacaatct atatcttcaa
    13141 gcgattcaaa aaatggatga aacatccgtt ttgcctggtg caatagctgt tcttgagtac
    13201 ttgaaaaaaa caaacatcaa gatcggattg ggatcagcaa gcaaaaatgc acgcctcatt
    13261 ttagaaaaaa cgaatttgac aagttatttt gatgtcctta ttgacggcac gcaagtatca
    13321 aaagcaaaac ctgatccaga agtttttctc aaaggagcac agcaattgaa tgtaccgcca
    13381 aatgcatgct tggtcattga agattcagaa gctggttgtc aagcagcttt ggctggtaac
    13441 atgcatgtat tgggaatagg tgaaaacata aatcttcctt ctgctgaata tgtgattcct
    13501 gatttaaccg tttttgatca agtacgttcg ttttggcatt tatcagaagc cgtatcacgt
    13561 atataatagt ttccgttttt tctgtcacaa gcaaaacgtt atcctccttt tggaaatcaa
    13621 ggtatgatga gaaaaaaggg ggattttata tgacaaaaaa agcagttttc gcaggcggat
    13681 gcttttggtg tatggtcaaa ccatttgaag agcagccagg gatcatttct gtgacatccg
    13741 gttatactgg cgggcatgta ccaaatccaa cttatgaaca agtaacaaca ggcacaacag
    13801 ggcatacaga agcagtagag atcgaatatg atccagaagt tatttcttat gatcagttgg
    13861 tggaaatcta ttggcagcaa accgatccga ctgacgcatt aggacagttc gctgatcgcg
    13921 gtgattctta tcgtccagtg atctactata atgatgaaga agaacgaaat attgcagagc
    13981 gttcaaaaca agcattgcaa gacagcgggc gattcgataa gccaatcgtg acaaaaatcg
    14041 aaccaagaag cgaattttat cctgcagaag attaccatca ggattattac aagaaaaaca
    14101 agctccatta caatctttat cgagaaggtt ctggacgtgc aggtttcctt cgtcgacatt
    14161 ggggcgtcaa gaaataagaa gccgaaaaag ctacttatgg aacaccgtat atcagaggtt
    14221 gtaaaaccag cattttgagc taatactgag ggtcagctga ttgaacacca tttagtggag
    14281 aggttgtgaa cctgtcgcct agctccaagt cacaaggatt tggtaagaga acactatata
    14341 tcagaggttg tgacaagtcg tttgtcacaa ccttttttca atacctaaga tgaaggacaa
    14401 tcagcaacgc actgctcaaa aaaggaatga aaggcagctt ttccaacttt tttttatgac
    14461 agataaagta aacagaaaaa acggatattc caaacagact agcaagaaac aagatctgaa
    14521 gcaactcttc tagagacaag ccaccactcc aagaaagcat caacagagta tcgccagctc
    14581 cgagatagga ttgaccatag cgaaggcaca caccgcctac gataaacgtt aacagcacac
    14641 tttgccattg gaagacgccg gcctgcaacc aaaaaatcca taaaattgcc caagaacaga
    14701 atagcgtgtg agaatggacc atgaagtaaa ataaatccat caacgaaaat agcaaagccg
    14761 aaagcatcca tatgaacgtc gaaaaatttc ttgcggaaga aaaagtaaag aaccaggcaa
    14821 aaagaccacc acataaggct tctgccaaca aatagtagaa gggcaaccga cacctgcaat
    14881 agcggcagcg aaatcgttgg acgatgatcg agagaatcgg aatcaattca taccaagaaa
    14941 gcgaacgaca gcatttgaca caatgagatc taggataaat aatagagtga cctaacggaa
    15001 tccgctgggc ggttaaacat aaaaatgatc cgatacagca accaatgata aaataaagta
    15061 acacgccatc tccccctttt caaaaccaag atacgcaagc ttaaactaaa tttttgtaca
    15121 tacataaaat ttgaggtgaa aaaatgaacg tttcaatcgg attagatatc ggaaccacac
    15181 aaaccaaagc agttgctttc acagatgaag cccaagaagc agcatccgct tattttcgct
    15241 accctttgat ccaagaaaca caagggatgg cagaacaaga tcaggaattg atctttcaag
    15301 gtgtctgcac cgttataaaa gaagtaatta aacagttgcc ttcagacaca atcattcaat
    15361 ttgtctcctt ctcctctgcg atgcatggcc tgatattgtt ggatgaagag aaaacaccgt
    15421 tatctcgaat gatcacttgg gcagataacc gagcagcgga cgaagcagaa aaactgaaat
    15481 ataccgtgct tggcaaagaa ttttatcggc agactgggac gccgattcat ccaatgacac
    15541 cattgaacaa aatcaagtgg ctgcaaaaca gccaacctga attattcaaa aaaactgctt
    15601 attttgtcgg tatcaaagac tatatctttt atagattgtt tggcgaactg atcagcgact
    15661 acggaacagc ttcaggaact ggatattttg atatccatga attccaatgg gcagaagaag
    15721 tgcttgacta cttgaacatt cgaatagagc aattgccgca agtctttcct tccacctatc
    15781 aagtaacagg tctaccatca aaaacagcag cagaactagg tttgccaaat gatatatcat
    15841 ttgttttggg aggggcagac gggccgcttt ctaatctagg gttaggtgct tttggtgata
    15901 cgatcgccgc tttgaccgta ggaacgagcg gagcacttcg ttttattact actcagcctc
    15961 agttccatcc agaaatggag acgttttgtt atgttctgga tcagaaacat tgggtagtgg
    16021 gaggagcgac tagcaatggt gctggcatct ttgattgggc agggcataca tttttacagg
    16081 aagtcgttca atctgctaaa gaaaaaggag aaaatccata tgatgcactg ctttcggcta
    16141 tcgcttttgt gccagcgggt gccaatggtc tgatttttca tccctattta cttggtgagc
    16201 gagcaccgct ttgggacgca gatgcgtttg gcagttttat cggtttacag cggcaacata
    16261 cagaaaaaga aatgatgaag gcgattctag aagggatctg tctcaacctt tatcggattt
    16321 taaaaggagt ttgccaggaa aatcagctca cagagatccg tgctacaggc gggtttgctc
    16381 aatctccgat ttttcgtcag atgatggctg atgtactggg ttacccgctt gttttcccta
    16441 ctgtcacaga agcatccgca ttaggagctg tgattttagg ctggagaagc atcggaaaga
    16501 tccaaagtat cagtgaagca caggaattga tagaattgaa agagcaagtt ggagtacaag
    16561 aaactgatca taaaaaatat cagaaaatct atccattatt tgtttcgaca caaaagcatt
    16621 tagcacagac ttatcagcaa tttgctcaac tgcgagaaga attagctgat tctgaaaaat
    16681 gattggcttt acggataaaa aagcgaactg aaaatcgttc tcaggtaaat gattttgttg
    16741 tacttttcat tgaaagtcta ctattatttt aagtgtaaga aaatgttagg aggcaagaaa
    16801 gatgaaattc caacaaacca aagaagtatt aaatcaatta gttgcagatc taagccaaat
    16861 gtctgttgtt gttcaccaaa cacattggta catgagagga ccaggtttcc ttacattgca
    16921 cccaatgatg gatgagttca tggatgattt gaacgctcag ttagatgaag tatccgaacg
    16981 cttgatcaca ttagacggtt caccttattc tacattgcgt gaatttgcag ataacacaaa
    17041 aatccctgat gaaattggcc gctgggatcg tactattcca gaacgtttgg aaacacttgt
    17101 tgcaggctat cgttatctag cagatcttta ccaaaaagga atcgaagttt ctggagaaga
    17161 aggcgacgat ccaacacaag atatctttat cgaattcaaa acagccacag aaaaacgtat
    17221 ctggatgatc caagctcatt tgggcaaagc gccagagatc gacgcgtaaa aactgttgtt
    17281 gaacttgacc gaaaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    17341 acgtcaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    17401 tgaacaccgt ttaccaagag gttgtaagcc tgccgttcag ctccgagtca caagaaacaa
    17461 ttttaaaaaa cagcttctta tgttttatgg atattgtgac ttgtgaccga ggagttggca
    17521 ggagaacacc gtttattcaa aataaggggt tgtgacaaga cttttgtcac agcctctttt
    17581 tgtttttcat tttgacagga aacgaccctt ttatataaaa gaaagaaaag aagtaaagtt
    17641 tcatataaat ttttcaatca agcagaaaaa cgttgacatt gactagatta gcgggtaata
    17701 tgttaaatgg tattgtttgc cccacaaaga gccccggaaa ctcgaagtgt atgtcaaaca
    17761 tcagaactta aattggagga aaataacgtg cgtacaacat atatggccaa accaggcgaa
    17821 gtagaacgta aatggtatgt agtagacgca actgatgttc cattgggacg tctttcaaca
    17881 gttgttgcat ctgtactacg tggtaaaaat aaaccaactt tcacacctca tttggatact
    17941 ggcgatttcg tcatcgtcat caatgcagac aaagtgaaat taacaggtaa aaaagcgact
    18001 gacaaaattt attaccgtca cagcaactac ccaggaggat tgaaatcaat cactgctggc
    18061 gaattgcgtg ctaaaaattc tcgtcgtttg atcgaaactt ctgtaaaagg tatgcttcct
    18121 aaaaacactt taggccgcaa acaatttaca aaattgaacg tatacggtgg agcagaacat
    18181 ccacatgcag cacaacaacc agaagtttta gacatcacaa acttaatcta ataggaggga
    18241 atttcattgg cacaagttca atatatcggc acaggccgtc gtaaaaacgc agttgctcgc
    18301 gtacgtctag taccaggaac tggcaaaatt actgtaaata aaaaagatgt tgaagaatac
    18361 attccacatg ctgacttgcg tgaagtaatc aatcaacctt tcgctgttac tgaaacaaaa
    18421 ggcgcatatg acgtatttgt taacgtaaac ggcggaggat acgctggaca atcaggagct
    18481 atccgtcatg gtatcgctcg tgcattgcta gaagttgatc ctgacttccg tttagctctt
    18541 aaacgtgctg gcttacttac tcgtgacgca cgtatggtcg aacgtaaaaa accaggtctt
    18601 aagaaagcac gtaaagcttc acaattctca aaacgttaat atcctttata tatcaacgtt
    18661 tctggcactt ttcaattatt tggaaagtgc ttcttttgtt tttaagaaca catttaagaa
    18721 cacatatcaa tcacacttat tcaatcctct ctgaaaagct tcaaaaacag ccttatcaac
    18781 tatatctggt gtgttcacat atatctttgt tgtagaaata tctgagtgtg ttaaagcatt
    18841 tgaaatttga ttcatatctg ctccaccttg tctagctata gttgcataag tatgacggag
    18901 tttgtgtgga gataaatgaa ctaagtcagg atgccttctt ttaattgaat tgattcgata
    18961 gtttaggtaa tcggcatgaa caggacaatt tacttctcca gatggtttag tataagtaaa
    19021 gagatactga tctaacgttt gcttaatttt taatttcaac aattgatcag cttgtaccga
    19081 tttccattca cttagcaatg tcattacaac ttctggtact tgaatgacgg tatcttttcg
    19141 accttttgta aacttttttc tttgatattt atctaaagta tgtttaagac ggaccgtttg
    19201 tttttcaaaa tcaatatatt tccattgcaa agcgtaagtc tcactttttc tatcaccaag
    19261 atacagggta atcataaaaa gtgtgtaatc atgaaaagta agtaaatgat tattaaaatc
    19321 atcatttact gcttctatcc aattagtaag ttcttttgat gatagtgctt gcttaccttc
    19381 ttgtattctt ttttcttcta aagcattttt tttaggtgca gtgatacttt gaataatttt
    19441 agtagttcga ttaaattcga tataatttag tatttctgca atatcgaaca tggaacgaac
    19501 atatcctttt atgattttta tatttgcata ttctttcgat tttttggtta attcatctga
    19561 tattatctca gtattgatat ttagataatt cattgcgtat tcaccaaaca taggtaaaat
    19621 atgcaaccga aatatatctt ttgtattact aatggttata tcactaggag gcctgtttga
    19681 tctaattgtt tgacctaatt catatctagg aagccattta gattcgtaaa attttttaaa
    19741 cgttattttt ccttttaatt ctaatgagtt tgcattctcc tctctcaaaa ctttttctat
    19801 tttcttcttc atatcatttt cggctttaat cgccaattgc ctatttggaa atgttttttg
    19861 atacttttct gatgcaattc ctgttatttc tttaaacctt tttggatatc tcagattagt
    19921 tttatatttc ccatttgaaa gctttgtagg caaaatcctc actccttaaa aaaagtaagg
    19981 cagtatattt cactaatttt agtatatgcg gcgagaagaa aatttcaagt tattttaaat
    20041 gtattcataa agtctttaat atcttctacg tcaaagcgaa ctacaccacc aactcttatt
    20101 actgggaaat tatatatacg aatccactta tctagagtat tattatcgat tcctagaaat
    20161 gcacaggttt gttttttatt caggtagact ctggattcta taaaatcatc tcctaaaata
    20221 gtagttatga aatttttgaa ttgctttggg taagcacata ggaatttcac ctctccccag
    20281 tttacggcga gccttacgga agtatcatta tttgctgcta ttgtcatggc attttgtttt
    20341 cccagaacaa aaagaaatct ctaagtattt gtcgctaatc aggtcatggc gtacttgagt
    20401 ttctagctcg aatagcaggg atcgttattc aaaaattgta tttaattgtc aatgatcaaa
    20461 aatagttttt ttcctattca ctattaataa cgataaacat gtataaaaag acacactacg
    20521 aaaaaagtta aatggagttt actgattttc atatttgtat atggaacaga catagtcttg
    20581 aataattttt ataaatgttt tgatgcttat caacttattc aacccgctat ccacttcaag
    20641 tataattttt ccatacgcca ataaagtagt tttaggagca tgttctcatt tgagaacgct
    20701 ccttttttat atattttgaa agctacttac tatacgcgta gtttcaaata gttttgtcat
    20761 tctaatcttc agcaacagaa aaaaatctaa actataaaat ttagggttga ctaaaatata
    20821 atttttgtat accatatgat cgtgataatc attatcattt agaaggagaa agttatgtta
    20881 gaagtgaaaa agcttaatgt ttgttataac gattttttag cattgaacga gatttcttta
    20941 aaaatacaag aaggtgcttt aacaggtatt attggtccaa atggtgctgg aaaatccaca
    21001 ttgcttaaag caatgttaaa tattatccct catcagggag aggtctccat taataatgaa
    21061 gatatcaata aaaaactggt gaagattgca tatgtagagc aaaaagctga tattgatttt
    21121 acttttccaa taaaagtaaa agaatgtgta tctatgggaa catatgccgg aatgaaagtt
    21181 tttcaaagaa ttaaaaatgc agaatggcaa agagtttcaa aggctttgga aaaggtcgat
    21241 atgggaaaat attctaatca tcaaatagga gaattatctg gaggacaatt tcaacgtgta
    21301 cttttagcaa gatgtcttgc tcaaaatgcg gatttcatat ttttagatga accatttgta
    21361 gggatagact tagttagtga acgaataatt atggatacgt taaaaaaatt gaaacatcag
    21421 ggaaaaacag tgctaattgt gcatcatgat ttaagcaagg taaagaagta ttttgataat
    21481 attattcttt tgaatcgaaa tctaattgct cacggaagtg tagaatctgt atttaatgaa
    21541 gaaaatttaa aaaaagccta tggagatact atatttattg gagaggggga ataatatgct
    21601 tcaaaatttt attaatggtc tgtatgactt tcacttttta cagaatgctc taattacatc
    21661 tgtagttata gggattgttt ctggaacagt aggttgtttt attattttaa gaggaatgtc
    21721 tcttatgggt gatgctattt ctcatgctgt tttgccagga gtagcaattt cttatatttt
    21781 aggtattaat ttttttattg gtgcgattgt atttggtcta ttagcttcta tcattattac
    21841 ttttataaaa agcaatagtg taataaaagg agatacagca attggtatta ctttcagttc
    21901 ttttttagca ctaggagtta tccttatagg agttgcaaat agctcaacgg atttatttca
    21961 tattttattt ggaaatattt tagctgtaca agatattgat aagtggatta caattggcgt
    22021 ttcggtttta gtgttggtta tagtagttct attttttaaa gaattattaa tcacttcttt
    22081 tgatccatta atggcaaaag caatagggat gaatgtcagt ttttatcatt atctattgat
    22141 ggttctatta actttagtag cagtaacagc tatgcaaagt gtgggaacta tattaattgt
    22201 tgcaatgtta attacaccag cagctacagc atatttatat gcaaatagct tgaaaactat
    22261 gatatttatt tctgcaacag tcggagcaag ttcatcattg cttggattgt ttattggtta
    22321 tagttttaac atagcagcag gatcaagtat tgttttaaca gcggcattct tatttgtaat
    22381 tagttttcta atctcaccaa aacaaaaatt tttaagaaag aaggagagat cgttatgatg
    22441 agaaaatgga aagcagtact gggaagtctg gggattttga ttgctctttt tatattcggg
    22501 gcatgttcaa taaatagtaa agacaaagat aaagtggctt cgaacaaaaa ttaaaagtag
    22561 tagttactaa ttcgatttta gcagatatta ccgaaaatat agcaaaagat aaaattgatt
    22621 tacacagtat cgtacctatt gggaaagatc cccacgaata tgaacctttg cctgaagatg
    22681 ttcaaaaaac ttcaaaagca gatttgattt tttataacgg tgttaacttg gaaactggag
    22741 gaaatgcttg gtttacaaaa ttagttaaaa atgcgaacaa agaggaaaac aaagactatt
    22801 ttgcagcaag tgatggcata gatgttattt acttagaggg tcagagtgag aaagggaagg
    22861 aagatcctca tgcttggtta aatttagaaa acggtattat ttacgctaaa aatattgaaa
    22921 aacagttagc ggaaaaagat cctgataata aaaaattcta taaagaaaat ctagataagt
    22981 atattgaaaa attggattct ctagacaaag aagctaaatc taaatttgct tcaattccga
    23041 atgataaaaa aatgattgtt acaagtgaag gatgctttaa atatttctcg aaagcgtata
    23101 atgtgccttc tgcttacatt tgggaaatca acactgaaga agaaggaaca ccagatcaaa
    23161 taaaacactt agttgaaaaa ttacgcacaa caaaagttcc ctccttattc gtagaaagca
    23221 gtgtggatga tagaccaatg aaaacagtat caaaagatac caatattcct atctattcaa
    23281 cgatttttac tgattcaatt gcagaaaaag gacaagatgg tgatagttac tatgcgatga
    23341 tgaaatggaa cctggataaa attgctgaag gcctttcgaa ataatttgga gggtaggtat
    23401 ggtacaaatt aaaacgacta ctatactaat ttatttacta gcaattcagg aattatcaaa
    23461 aagaaaaaag aaattaaaaa ataatgattt agcaaaagtt ctaagtgtta gtccagcatc
    23521 tgtaagtgag atgctggcta aacttagata tgaggactat ttagatatag gatttcaatt
    23581 aactgataaa gggaaaaaat ttattgacga ttacaaaaaa ggttttaagt aaaagacttt
    23641 atttttaggg aggataagaa aatggctatt ggagtgatta tgataggact tatcctaata
    23701 gctggatata atttttttaa atctttctcg gattatttta gaaaaggtga atgatttaga
    23761 tgagtctatt aattcttgta ggattgcttt ttgtatcagt tattctgctc tttctcatta
    23821 gtttagctct aaggaatgag ttcagtaata aacgttaacg attgaaactt caagtcctgt
    23881 gtaagataat aaagttaatt catcttatta ggaggaagta cccatagaaa ccagagatta
    23941 tattataact caccaaaagt taatagaaag tggactaagc cattttgaaa aagaaggatt
    24001 tcagcaaact aagataaggc aactgtgcct gaccgcaggc gtaactacgg gtgcttttta
    24061 taaacatttt acttctaagg aagcgttatt tgaagaaatt attcagccgc atattaatgc
    24121 gcttattaat atgtataatg aagagtttac caaatacctc ttaaacttag aaaatataga
    24181 tatagagaaa agttggagag agaacttcaa tgaattagat ccatttatta attatatttt
    24241 tgagaataaa cgtatattcg atttgatttt attccaatca gctggaacca aatatcaaga
    24301 tattgttgaa aaaattacat catttgtgac gaaaaagaca gtctgttgtc tagataagtt
    24361 aaagactatc ggaatg
//
LOCUS       DASJTC010000039        24333 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.039, whole genome shotgun sequence.
ACCESSION   DASJTC010000039 DASJTC010000000
VERSION     DASJTC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.039"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(79..447)
                     /locus_tag="U4B19_001398"
     CDS             complement(79..447)
                     /locus_tag="U4B19_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEN2077146.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            618..1574
                     /locus_tag="U4B19_001399"
     CDS             618..1574
                     /locus_tag="U4B19_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2077147.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1647..1763
                     /locus_tag="U4B19_001400"
     CDS             1647..1763
                     /locus_tag="U4B19_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEN2077148.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1818..3500)
                     /gene="recN"
                     /locus_tag="U4B19_001401"
     CDS             complement(1818..3500)
                     /gene="recN"
                     /locus_tag="U4B19_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEN2077149.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(3518..3967)
                     /locus_tag="U4B19_001402"
     CDS             complement(3518..3967)
                     /locus_tag="U4B19_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN2077150.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(4074..4892)
                     /locus_tag="U4B19_001403"
     CDS             complement(4074..4892)
                     /locus_tag="U4B19_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEN2077151.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4901..5791)
                     /locus_tag="U4B19_001404"
     CDS             complement(4901..5791)
                     /locus_tag="U4B19_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN2077152.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5793..6017)
                     /locus_tag="U4B19_001405"
     CDS             complement(5793..6017)
                     /locus_tag="U4B19_001405"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEN2077153.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(6022..7359)
                     /locus_tag="U4B19_001406"
     CDS             complement(6022..7359)
                     /locus_tag="U4B19_001406"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEN2077154.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(7360..8214)
                     /locus_tag="U4B19_001407"
     CDS             complement(7360..8214)
                     /locus_tag="U4B19_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEN2077155.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(8311..8760)
                     /gene="nusB"
                     /locus_tag="U4B19_001408"
     CDS             complement(8311..8760)
                     /gene="nusB"
                     /locus_tag="U4B19_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEN2077156.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8753..9178)
                     /locus_tag="U4B19_001409"
     CDS             complement(8753..9178)
                     /locus_tag="U4B19_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2077157.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(9424..10488)
                     /locus_tag="U4B19_001410"
     CDS             complement(9424..10488)
                     /locus_tag="U4B19_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEN2077158.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            complement(10660..10905)
                     /locus_tag="U4B19_001411"
     CDS             complement(10660..10905)
                     /locus_tag="U4B19_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2077159.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            complement(11044..11418)
                     /locus_tag="U4B19_001412"
     CDS             complement(11044..11418)
                     /locus_tag="U4B19_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEN2077160.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            complement(11501..12415)
                     /locus_tag="U4B19_001413"
     CDS             complement(11501..12415)
                     /locus_tag="U4B19_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEN2077161.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            complement(12436..13239)
                     /locus_tag="U4B19_001414"
     CDS             complement(12436..13239)
                     /locus_tag="U4B19_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEN2077162.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            complement(13272..14261)
                     /gene="manX"
                     /locus_tag="U4B19_001415"
     CDS             complement(13272..14261)
                     /gene="manX"
                     /locus_tag="U4B19_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEN2077163.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            complement(14364..14855)
                     /locus_tag="U4B19_001416"
     CDS             complement(14364..14855)
                     /locus_tag="U4B19_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2077164.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            complement(15046..17853)
                     /locus_tag="U4B19_001417"
     CDS             complement(15046..17853)
                     /locus_tag="U4B19_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2077165.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            complement(18287..18580)
                     /gene="rpmA"
                     /locus_tag="U4B19_001418"
     CDS             complement(18287..18580)
                     /gene="rpmA"
                     /locus_tag="U4B19_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEN2077166.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(18593..18937)
                     /locus_tag="U4B19_001419"
     CDS             complement(18593..18937)
                     /locus_tag="U4B19_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEN2077167.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            complement(18964..19272)
                     /gene="rplU"
                     /locus_tag="U4B19_001420"
     CDS             complement(18964..19272)
                     /gene="rplU"
                     /locus_tag="U4B19_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEN2077168.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     misc_feature    complement(19292..19368)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            19554..20195
                     /locus_tag="U4B19_001421"
     CDS             19554..20195
                     /locus_tag="U4B19_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN2077169.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            complement(20257..21888)
                     /locus_tag="U4B19_001422"
     CDS             complement(20257..21888)
                     /locus_tag="U4B19_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEN2077170.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(22011..22370)
                     /locus_tag="U4B19_001423"
     CDS             complement(22011..22370)
                     /locus_tag="U4B19_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEN2077171.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            complement(22529..22602)
                     /locus_tag="U4B19_001424"
     tRNA            complement(22529..22602)
                     /locus_tag="U4B19_001424"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22566..22568),aa:Arg,seq:tct)
     gene            complement(22911..23594)
                     /locus_tag="U4B19_001425"
     CDS             complement(22911..23594)
                     /locus_tag="U4B19_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEN2077172.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            complement(23722..24240)
                     /locus_tag="U4B19_001426"
     CDS             complement(23722..24240)
                     /locus_tag="U4B19_001426"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEN2077173.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
BASE COUNT         7125 a         5312 c         4013 g         7883 t
ORIGIN      
        1 ttttattcat aaacattttt ttaaaaatca agctgcaaaa agccttttaa aagacttttt
       61 acagcttttc tatttttttc ataggtaaga aagtatgttc acaatgatca agacagcgta
      121 caagacgatt gtcaacagaa aaaccaaacg ccaagtcatc ttgaaataac gcggataaat
      181 aatttcttca taataatatg cttggaaaac agcaatacaa attcccatta gcaaaacaga
      241 aataaagaaa aacggtaaga tagaaattgt ccctgtattt ctagacaatg catgcatacc
      301 gccccacaaa aaaggaatcg ccaaatcagg ggcttttatt ttaaaacgac gatttaacga
      361 aaataatttt acaagaaatt ggcaaccaaa caaaacgatt accgggtaaa tataccaaaa
      421 caagataagt ggtgtgaatg tgcccatgaa cgtaaactcc ttttttatcg ttgcttcaag
      481 tatactgtat atactttaca caactgtttc attttattta aatttatgca gttatattct
      541 atatcttaca aacacattcc atgtattcca attccctatc cgttataata aaagaaacag
      601 gcaacaggag gaaaatcatg atcaaatatt ttacattaga taatgagaaa ctgcgtaata
      661 ccggaaagga aactgaccaa acgatctggt attgtgtaga acgtcctaat caagaagaga
      721 ttcatcagct gacacatcaa tttgaaattc cgtcagatta catcacttcc atattggacg
      781 atgcagaaaa ttcccgtaca gaagaattca atcaatcaaa gttgacaaag ccagcgttat
      841 tattgttaca atatcctttt gctaaaacaa gtcctagcgg ttattttcaa gtggatacat
      901 acccgctctc tattattttg acgcccaaaa agaagcttat cacaattacg aatcatgaac
      961 cactgttttt aaagaaagtc tttagccaga cctttccaaa aagtgatatt tcgcctaatc
     1021 tcaatatctt ctttcaattc atttggcagg tcacactctc ttacaacagt tatttagcta
     1081 cgcttcaatc tcaatgcgat actttggaag ccaaacttca agtatccact gaaaacaaac
     1141 aactttatca aatcatggat atccaaaaaa gcctcgttta tttcagagaa gcgacttctg
     1201 ctaatttaga tgcattgaaa gaactagcgc aaaataaact tttccaagaa aatcatgctt
     1261 tgaaaaatca tttacgtgat gtattggtgg aaacagagca ggcaatgact acttctcgca
     1321 ttcaattaaa actagtagaa caaatgaatc agactttttc agcaatcgta tcgaataatc
     1381 tgaataatat tatgaaaatt ttaacttctt taacgattat tttaactata ccgacaatcg
     1441 tcggcagtat ctatggaatg aacattaagt tacctattgc tgaaagggat gatgccttta
     1501 tatggctagt tctattaaca ttatttattt gtatcgtaac tacctattat ttaaaaaaag
     1561 ggaagttttt ataaaaaagc cctcttgtgg aaatggaaaa aagattgtac acttgaatca
     1621 actaattaat gacgaggttg aaaacaatgc gtgaaaaaaa acaaacaagt acttttgcta
     1681 aagtaacaaa atttgtcatt tggacaatgc tgattttaac gattggatct gttgtattga
     1741 ctgctgtaat gagtgtcatg taaaaataaa atatagctat cagctgctga aaaggacctg
     1801 aaccactgcg aggtggctca ggtccttttt gcgagtttca atagttcttt tgcatgttcg
     1861 agtgtgagtt ttgtaatctc acttccagcc agcatacgtg caatttcttc ttctctttct
     1921 tttgttttca actctgaaac agaagtttgc gtacgtccat caatcactgc tttcacaata
     1981 taatattgat agtcggcgac tgcagcaact tgaggcagat gagtgatgca caaaacttga
     2041 gagtatttcg atatttgaga aattttatct gcgatggcct gtgcaactct tccgctcaca
     2101 cctgtatcca cttcatcaaa aataatactt gtcacacctt gctcagaaga aaaaatagtt
     2161 ttcaaagcaa gcaacatccg agacagttcg ccaccagaag cgactttcac caaaggcttc
     2221 agtggctctc ctggattggt cgtaatgtaa aattcgattt ggtcaaatcc atcatgattc
     2281 aattgtctat tttcttcttt taagaaacgt acctcaaact cagtgttttc catatacaga
     2341 cttttcaatt catgcagaat ggatttttcc aattcttttg cgattttttt acgcgcttca
     2401 tgaagatctg cggcttgttg ctgaagtaga agctcttttt gctctagttc ctctttcatt
     2461 ttgtccaatt gaccttctga aaaatcagaa gaagctagct cttcggtgat ttcgtcatag
     2521 taagccaata ttgactcaat ggattcgccg tatttacgtt tcaactgtcg gattaattct
     2581 aaacgttgcg taacttcttc taaacgactc tcatctaact ccaataaatc aatctgacga
     2641 ctcatatcgc cgattgcatc ttgcagcaaa tagtaagcac tttggatatt ttcataaatt
     2701 gcttcatact ccacatctag atgggcgatc ccttgcagtt ctgaaacggc caagccaaca
     2761 ccatctacac tgttttgttc attattcgtc agtgctccgt aacctgcagc taatccatca
     2821 gcaatttttt gataattgct aagtttttct ctttcttctt ttaatttctc ttcttctccg
     2881 acttgcaaat cagcagcagc tatttcttct tgctggaaat ggagcatatc tattctctga
     2941 acataggatt tttcattttg ctgcactttt cgtacccttt tttccagctc acgataagag
     3001 atatattctt gctgataact ctttttcttc tgttggaaag ctacatctcc aaaacgatcc
     3061 aataaagcaa gatgtgattc aggctggagc agttcctgat gttcgttttg tccttggata
     3121 tctactaaat aactgccgat tcttctcaga ttagctaatg tgataatatg accattcact
     3181 cgacaaacat tttttccagt taaggacata tcccgtcgaa caatcagatt gtcctcatct
     3241 gtttcgatcc ctaattcaac catcagttca gagaatcctt cttgttttgg tagttcaaac
     3301 aacccttcaa gcacacattt ttctgctcct tgacgtatat aatcgcttga acctcttcct
     3361 cctgctaaca gacctaaagc atcgataata atggatttac ccgcaccagt ctctccggtc
     3421 agcgcggtca ttccttcgtg aaaagaaagc cgcaattccg gaataatcgc aaagttagta
     3481 atactgattt cttgtagcat cacattcgct cctcaactta aaggtaatgc aggaagtctt
     3541 tcttcaataa aactgcctgg tcttcagttc gtgtaatcat caaaacatta tcgtcatcat
     3601 tgataatcga aaataattct ttcttgtaac gtttatcaat caaatttgct gctgccgaag
     3661 catttcccgg caatgttttc aaaataacaa atttttccat ttggtcaact gccacgaaag
     3721 cgtcttttaa tagtttttcc aatttagtat ttacatcttc gtttgtttca gctggtatgc
     3781 tataacggta gccgccatct gaagccggta ccttgatcag tttcagttcc tttatatctc
     3841 tggaaatcgt agcttgagta acagatattc ctttgctttt caggatttca acaaattctt
     3901 cctgctttcg gatatcttgc tcactcaaca atcgagtaat caaacgatgc cgatcttttt
     3961 ttctcattac gtcttcgctc cttagcatat tcttcttaat caatgatagc ttatttcctt
     4021 ggtataataa agaagaatcc gcatacttct cacttgattt tcacagttac aatttattta
     4081 tggtcaaact gttcgtgtgc atttgaaacc acttctgcca tattaatagc tgaattgatt
     4141 gttccgcttt cgggtacttt cctcaagtga gccaagaact cgatatttcc ttctcccccc
     4201 gtgatcggag aataacttaa ttccagcaca tcatatccac cttgtgccgc aaatgacaaa
     4261 atgtcttcta aaactttttg atgggtctga gggtcacgaa caattccgtt tttccctaca
     4321 gcttctctgc ctgcttcgaa ttggggtttg attaaagcaa tcacttctcc ctcttctttt
     4381 aaaatagcat gtaatggagg cagcatcagt tttagtgaaa taaaagaaac atcgataaca
     4441 gcaacttctg gaattcctaa ggtaaagtct tcgggttttg cataacgaaa attcactctt
     4501 tccattacga cgactctagg atcttgtctt attttccaag ctagctgatt gtagccaacg
     4561 tctaaagcat aactcatttt tgcgccattt tgcaaagctg catcagtaaa tccgccagtg
     4621 gatgcaccaa tatctaacaa tgtttttcct tcaacagata gatcaaatgc tttgagtgct
     4681 ttttctaatt ttaatccccc tcgagaaaca taaggaagtt ttttaccttt gactcttaat
     4741 tcactactca ctggaatttt ttctcccggt ttatcaaacc gctcgttttt ttcgttgtaa
     4801 atcaaaccag ccatcactga acgtttggct tgttctcttg tttcaaataa tccttgtttg
     4861 accgctaata catctacgcg ttctttttcc attctttccc tcatttctcc acatcaaatt
     4921 gcttgatcat ctggcgcatc agcccgccat caaaagaaaa aacgtcctct tctaatttgt
     4981 ctatgatctt gttggcggat aacagctcaa tctccaacgc ctcacgtgtc ttttctaggc
     5041 ctaataaacg cgggtaagta tttttttcca tacgttcatc tttaccagct gttttcccta
     5101 atgttttcgt tgtaccaatc acatcgagta gatcatctct tatctgaaaa gctaaaccta
     5161 aatgttcggc taatcgttga agtagcaata agatttcttc aggttgcttc gctaatatgc
     5221 caccagccag caaggcataa cgaatcaaat gccctgtctt tctttcatga ataaatgcta
     5281 attcttctaa agaaagcttt ttcgattcgc cctcgatatc tgcagcttgc ccagcaacca
     5341 taccttgtga tcccgcacaa acagctaatt gctgaagcag cagaagctta ggagaattac
     5401 ctaagtgtgc catactgatt agctggaatg ctcctgtaag caaaccatct ccggccaata
     5461 tagctaatgc ttcgccgaat actttatgat tggtaggttt tcctctacga agatcatcat
     5521 tgtccatcgc aggcaaatca tcgtggatca aggaatacgt atgcaccatt tctaaagcag
     5581 ctgctacttg atagacggga acatcaatag gctcatcaaa ggaagcaacg gccgttaaaa
     5641 gtaataaggg acgtattctt ttcccgcctg ctttcactga ataactcatc gcatcataga
     5701 gtcctctatc cgttgtatgc tcatctaaaa aagacaagat ttctttttct acagcaggta
     5761 gatgaatagc tgaaaagtca gttaatctca tctcagcttt cctcgtttcc gtcaaaatca
     5821 acttcttcat ttgattctgt catcattttc gtcaaggtct cttctgcttt agctaatgta
     5881 tcttggcaat gtttgcttaa ttccattcct cgtttaaacg aatctagagc tgattctaat
     5941 ggaacgtctc cttgttctaa ctgcatgaca attttttcta attcttgcag tgactcttca
     6001 aaagttggat tttccatatt tttattctcc tttgtctatc tcttgtactt gtcctttaac
     6061 gattccatct tgataatgga tcgtcagcaa atccccttga tgaatctcgg tgacactttt
     6121 tactacttga tcatttaacg tcgtatagct gtagcctctt cccatgattt tcaatggact
     6181 caacaaatca agagattgaa cagcttgagc aaaacgctgc tgctgttgtt tcatatattg
     6241 ttggattttc tcttctaagc gtttttctag ataagcagtt tcttggcgag ctgctttaac
     6301 tcgataaagc ggagccactt gttccaattg gtggcttaat gcgatcgttt gtttttcttt
     6361 cgcatgataa atttgctgtg tagcttgata taatcgctgt ttcaactggt ctaatttgat
     6421 ggtctgacct tcatacaatc tttcaggctg ccgaaataca taggaacgtc gagcacgctc
     6481 gaaccgttct tgtttgcgct ggatctggcg tacaaacgcc tgctgcaatc tagcttgccg
     6541 ttcttgaatg cgcatcaact cttctgacaa aacaggaaca gctaattctg cagctgctgt
     6601 tggagtggca gcccgaacat ctgctactaa atcagcgatc gtggtatctg tttcatgtcc
     6661 tacagatgaa atcaccggcg tattagcttg ataaatcgcc cgagcaaccc gctcttcatt
     6721 aaacggccat aaatcctcaa tcgaaccacc cccacgaccg atgatcattg tatcgaaatt
     6781 gcccatctct tctactctct ggatattcct gacaacattg tccgctgctt ggtttccttg
     6841 tacaactgtt gggaacaaaa ccaattggac aattggatat cttcttttca ccgtcgtgat
     6901 gatatcgcgg ataaccgctc cgcttggact ggtaaggacc gcgattcgct ttgggaaacg
     6961 tggcagcatt tttttaggcc cttgaaaaag gccttccttc atcagctttt cttttcgttc
     7021 ttccaattct tgatataaag cacctattcc atcaggctcc atgtgctcta catagatttg
     7081 ataagaacct cccgcttcat ataatgaaat acgcccaacg acaaggactt tcattccttc
     7141 ttttggctgg aatcgtaatt tttggaatgc tcctttgaac ataatagcag atatttttgc
     7201 atggtcatct tttaagctga aatattgatg ggcattggca cgtaaacgga aattcgatat
     7261 ttcgcctgtt aaatatacgc gttctaaata aggatcggca tcaaattttc ttttcaaata
     7321 cttcgttaat gtagtgacag ttaaatattc ttgtgccatc tattcacctg ctccaaaagc
     7381 ttcggcagat tttacggttt gatacatcag catcgtgata gtcattggcc ctacaccctt
     7441 tggcacagga gtgatccagc ctgcaatagg ctccacttca tcaaatttca catcaccgat
     7501 cagcttaccg ttagcatcac gattcatccc tacatctatc actacagcac ctggttttac
     7561 gaattctttt gtgacaaaat gtccacgtcc tattgctacg actaaaatat ctgcttcttg
     7621 tgccagttca gccagatttt cggttttaga atgagcaaca gtaacagttg catctgccat
     7681 caagagtagc tgagccatcg gtttaccgac gatattgctt cgtccgataa ctactgctcg
     7741 tttacccgcc ggatcgatag aataagcttc aaacattttc atgatgccgt atggagtaca
     7801 aggaatcttg tccggtttac ccgcaaggag tctgcctaaa ttcatcggat gaaaaccgtc
     7861 tacgtctttt tcagggcgga tagctaacag gatcttttct tcatctatat gctttggcaa
     7921 tggtaattgg accaatatac catgaaaact aggatcttga ttgtattttt caatttctgc
     7981 aagtagttcg tcttcagaaa tcgtctcagg ataacgctct accaatgaat ggattccgat
     8041 ttcttttgct acacgttctt tatttcttac atagatttgg ctggctggat tttcccctac
     8101 caataaaacg actaatccgg gatggatacc attatctttc aatttttgga ttttttcagc
     8161 aatctctgcc tgcatttttt cagctaattc ttttccgtta atcaattctg ccatctaacc
     8221 actcccagat attttttgtg ttttcattct accatagaat tttcccgatt tataacaggt
     8281 ttatgatgat tcatagaaaa aagctaggac ttacgcccca gcttctaatt ctttcaatac
     8341 attggataaa acaccgttga cgaatttacg tgaacggtca tcactaaatg tttttgccaa
     8401 ctcgattgct tcatttaaag caacagccgc tggtacatct gtcacataag tcatttcaaa
     8461 aatcgcgata cgcaagatca ccaaatccat ttttgagatc cgattgatgt gccaattgtt
     8521 ttttaaatgt ttttcaatca ctttatccaa ttcatctttt tttgcacaga caccgccaac
     8581 tagcgtatca agataaacag gaacaaactc tgattcatct tcgttgatca tgtcgcggtg
     8641 gtctaattcg attgcgttga aaatcgcatc ttcttttgtt aggtctgcat tgaagtctaa
     8701 tggaaataaa gcctgcaatg ccatttcccg aatctcgtgt cttgataatt ctttattcat
     8761 tctcttcctc ttcgtcttca aaaagttctt cgattgttgg ttgttctaat ttttcaggaa
     8821 caacagctac tacatggata ttgacttcag ataaatcgat atccgtcatg aaaaggactt
     8881 gctggctgac ccgttcctgc atttccattg ctactttagg aactgaaaca ccatattcta
     8941 gataacagta aagatctact ttcaagccat cttcctcgtt tgctaagtaa acaccttttc
     9001 cgtgagctgc acgtcctaat aattcagtga cattgctggc aaaagttccg cgcattccgt
     9061 atacgccttc tactttagaa gctgcaatcc cgataattac ttcgatgact tcaggagcga
     9121 tcacgatctc tcctaaatct tggtttgcgt ttaaaatcaa atttttttca tctgccattt
     9181 atttattcct ccctttgcct aagcacgaga tacatccgaa ttctctatga tgcggcatag
     9241 tgatcacgtg gtaagaaaac tgccgccgat cagctgcaaa ctgctatgaa ttggagaaaa
     9301 cacccaactt tattatctct tacagcttaa aagttcttct atagtgtatc attaaatcga
     9361 gtgattttct ataacaaagt ctgtgaacac tgatttttac gcttacttct tctattgatg
     9421 aaactataag atgatcaatt cttttggtga acgagtcaac acacgatttc catcttctgt
     9481 gatcaacaag tcatcctcaa tacgtactcc accgattcct ggtaagtaga tccctggttc
     9541 atcagtgatc acatttcctg gtacaaattg cttttctgca cgagaagaaa cgtttggtcc
     9601 ttcgtggatc tctaaaccga tcccatgtcc tgtagaatgt ccaaatgctt ccccgtaacc
     9661 atgttttgaa atataatctc tagcaactgc atctaattgg acaccagtca ttcctggttt
     9721 tgcagcatca ataactgcta attgagcatc taagactatt tgataaattt cttttaattt
     9781 ttccccagga tcaccaatag cgaatgtacg tgtcatatcg gaaacgtacc cttcgtaata
     9841 gcaaccaaag tccaatgtga tcaagtcacc ttgttcgatg atttttttgc ttgctacacc
     9901 atgcggcata gccgaacgca atccgctagc aacgatcgta tcaaaagaaa cacctgaagc
     9961 gccaagagaa cgcatataaa agtctagttg atttgcgact tcaatctcag tcatgcccgg
    10021 ttggatcatt ttcaggatgt gatcatatgc catatccgca atatgacatg ctttttcgat
    10081 aatagcgatt tcttcttcat ctttcacttc tctgagttct tcgatcatct gtgaaacagg
    10141 tactaattcc gcatcgatga tttcttctaa aaccgaatat tctaaaaatg agactgttgt
    10201 ttcctcaaaa gctaatgagt gcagttcttc tttgcgtact aagtttgcta cctcatcaaa
    10261 aataggtccg acatttttaa taatttcaaa cccttgagcc tgcgctgccg cctgctcggt
    10321 ataacgaaaa tcagtaataa aaaatgcttt ttccaaagta ataacagcta atcctgtcgt
    10381 acctgtaaag ttcgttaagt aacgaagatt gtacggactg gtaaccaaaa atgaatccaa
    10441 attttcttct tgcatttttt tgcgcaattt ttctacacgt agcatcataa tgaaactccc
    10501 tctttctttg cacgcttttt tccattatag caaacttttc ttcgtacacg acagttaaat
    10561 aaaaatttcc ggaaaacttc tttttatttt aaataaataa agccaaaaag gagtcacagt
    10621 ctcgctgcaa ctccttttag tttactattt tgtaaaaact tatgatcgtt ttccaaagaa
    10681 aaatgagaca actgctacca aaatcactgc gcctaaaata gatggaataa gtgccatacc
    10741 tgcagctttt ggtccccatt cgccgaaaat cgcttgtcca atacttgaac cgactagtcc
    10801 agcaatgata ttcgttacac agcccatcga atttccttta ctggtgatcg cacctgcaat
    10861 tgctccgatc acggctccaa caatcaatgt ccataaccaa ctcataatat aacctccttc
    10921 tttgctaatc aattttagca tatgggattt aaaggggaca aataattggt tttgctattt
    10981 catattttct tctgcaaagt aaaaaaataa agaaaacctc ctcttagaag ttttctttat
    11041 tttttacttg cgtttaaaca tactacgaat accacgtttc actacatcaa agaaactcaa
    11101 tgattgtacc attctatctt catctacata ttctctgatc gcacgaagca cttttttagg
    11161 ttctttggaa gaaaatgtgt atgtaccatt ttttttcgtt tgaatggcat atcgaggaat
    11221 ccattttccc tttagcatga ctgatgctag tacataatcc acttcttccc atggaatttg
    11281 gatatatttg cgtctatctc gcgcatggta aaactcaaaa ccattattac ctatcatgat
    11341 ttgcccgtaa tctgtcagac ccgtaaacga agtagcatca attaccaagt ctacttttgt
    11401 attgattgat tgaaccattt tctccctcca gatatttgtt tatgaacagt atactatata
    11461 aaaaaagctc gggacaacgc ccgagctata tttttatgga ttataaaagt ccgatgacgt
    11521 gtccaacaac accgactacg aataaaccaa ggatgataac gattggagaa acctttttct
    11581 taagcaacca catacataac aatgttaatc ctaatgcagc taaaccagga atcaattggt
    11641 ctaagttgtc ttgtaatgtt gttacctttt caggagaaag ggctaagcca ttattaactt
    11701 gttcaaatgc actcttgatt ccttcaccac ctgaaggcaa tttatcccat tcgatgtaag
    11761 cacctttatc taattttaca gttgatacaa ctggtaagaa tttgattgat acccatcttt
    11821 gtacaagggc agctaatacg aacatcccta aaattgaagc accttttgtt acgtcttgta
    11881 acaatccgcc agaaaggtca tcagtaattt tagaaccagc tttgtagcca aattcttgtg
    11941 tataccacat gaatgcccaa cgaataatgt tccaacctaa gaagaagatg attggtccaa
    12001 ggatatttcc tcccatagct aatgaagcac ctagtgcacc tagcattgga cgtactgtaa
    12061 accagaacac tgggtcacca acaccagcta aaggacccat catacctact ttaacccctt
    12121 gaatcgctac atcatcaaca ggcgcgccat tcgcacgttc ttcttccaga gccaatgtaa
    12181 cccctaaaat tggagaagca atgtatgggt gtgtattaaa gaattccaag tgacgtttta
    12241 atgctgcaga acgttcttct tttgttttgt ataatttttt gattgctgga atcatagaaa
    12301 atgcccagcc accgttttgc atacgttcgt agttccatga accttgaatg aaggtagagc
    12361 gccacgcaac agctaaacga tctttttttg ataatttgat tttttcttct gccattttat
    12421 tttcctcctt caagattaat agtcattcaa aatgtcgcct aaagggtcac cagagttgct
    12481 tccgcctcca ttatttgatg aaccacccat tttagaaagg ttcaagtaaa taagagctaa
    12541 tgcaacacct aaagcaccta gagcaatcaa tgttaattga gaaattgcag ctacaacgaa
    12601 accaataacg aagaatggcc atacttcttt tgtagccatc atgttgatta ccaaagcgta
    12661 acctacagct acgaccattc ccccaccgat agccatacca tctgttaacc atgctggcat
    12721 tgattcaagg aatgattgca cagtttgtgc tgggataaat agcaacgctg ctgctgggac
    12781 cgcgatacga accccttgta aacatacagc aatgatgtgc cacatttcaa ccttacggat
    12841 gtttccttct tcagcagccg catccatcaa atgaacgatt ggaacagcga ttgtacgtac
    12901 gatcattgtt aagaacaacc ctgcaacagc caaaggaacg gcaatcgcga tcgcagaagg
    12961 aacaccttta acaccttgtc cacctaaaac taaaataatt gctgatgcta ctgatgctaa
    13021 tgcagcatct ggtgctacag cggcaccgat atttgcccaa cctaaagcga tcatctgaag
    13081 cgtaccgcca agtacgatac cagcttcgag gttaccggta actaaaccga tcaatgtaca
    13141 tgccactagt ggttgatgaa attgaaattc atcaagaata ccttccatac cagctagaaa
    13201 ggcaactaat acgactaaaa ttattgaaat aatagacatg ataagcctcc tataaatttt
    13261 aatattattt attacgcttt tgctaattca gcttttgctt ttttcaaaat atcatccatg
    13321 ttatcgcgtg aatcattagg tactttacgt acatcgaatt tcacaccttt ttgttcaagc
    13381 ttctcaaacg cttcaacgtc ttcttgtccc atagacaata ctttacttac aacgacttta
    13441 ccgaccgaat gcgccattga cccaacattt aattctttga tgtctacgcc accttcaaca
    13501 gctgtcaata cgtcttcagg attttcaaat aaaagcaatg ctttggtatt tccaaaacga
    13561 gggtcttttg ctacttcgat cattttgcta ataggaataa catttgcttt tacacctgga
    13621 ggagcagctt gttcgattag tctcttacgc agatcatcct ttgatactgc atctgaaaca
    13681 acgataatac ggtttggttg cactgctttt gtccaagctg tcgcaacttg tccatgtaac
    13741 agacgtgagt caacacgtgc taatacatat ttgatttttc cgtctccgac cacagttcct
    13801 tctggaagtg ctcctttagg ttgtgcgtct tcaactgctg ctttcggtgc ttctgccggt
    13861 tctaattctt ccggttttac ttttacaccg tcttttgctg tttcaatgat gtgtgctgca
    13921 atttcatgag cagattccat tgaaaagcgt gatgcgtagg cttcaatcaa cattggtaaa
    13981 ttcaaaccgc tgacgattgc ccatttatct ttgtgctctt cgaataatgt gtttgattga
    14041 ttgaacggtg ttccgcccca taaatcaact aagaataaca cttcgtcttg attatcaaaa
    14101 gaagcaaccg cttcttccaa tttggctctc aagtcatctg gaccttcact tggtttcaaa
    14161 atcacggctt tgacgttttc ttgttcgcca aagatcattg aaccggattg taagattcct
    14221 tcagcaaatt gtccatgact tgctaggata atccctacca tatttatacc tcctatttac
    14281 ttttgttgta acaaattcaa aaatattaat aattgctaac cattacaaca gaattatttg
    14341 accttttcca acaaccattc ttgctacacc tttttgattt tcgctagtag ttccatcaaa
    14401 aggattttcc tgtcagcagg tacttttcga atctctaact ccacttcttg actgttcaag
    14461 taacgaaagg aatcaatatc ttgttgattt actgaaacaa aattggtaat cattgatttg
    14521 ccttcagtaa aactcatacc gccgatattc aacgacttca actgtacacc gcagcggaca
    14581 accgcttcaa catcttgggg attggtgaat aaaagcatca cttttgctcg tgataataat
    14641 ccactatgat agatttgaat cattttctgc acggttatta catttgctgt gatccccggc
    14701 ggtgcagctt cttttagtag aaactttctc aatttatctt gagcgactac atcgctgatc
    14761 acaatgatac gttcaacacc tgtttgcttc gaccaaactg ttgcgacttg cccgtggatt
    14821 aaacgatcat ctatacgtgc caatcgaata tccatctgca aaactccttc ttcctcacta
    14881 cttcacagaa acccctatca tttctgtgtg aacaaccacc tcctagcaca cactactata
    14941 agcaaaaagc gtgccaactt ttttacgata cactaattga atttaaagga ttcttaacca
    15001 atacacaaaa acgatacact ataaaagtgt atcgtttttg tgtattcatg gcacagtgta
    15061 ttctttttta tctaactgat tatggactag acgtagcaga taatacattt cttctatcgg
    15121 cacttttact tttatctgat cttccaacag aacaactgag gctgccaatt ggctataaag
    15181 aggatcattc gttgtatttg tcaattcgtt ttcttccaca gtcaatggtt cattccgaat
    15241 aatacgttcg atcattcctg ctgtgtgcat cactaggttg attttgaatc catattcttc
    15301 atcgccgatt cccgtcgtct gagagatttg tgtagaaaat tgccacattg gatcgattaa
    15361 cttactacca ttaataaaga tgaagttatc agagataaac tcaacacacg tatttttagc
    15421 tgactcttca tctagggaga tgaattcagt ttcctccaat tctgattcca tcagcaactg
    15481 atccaaaatg acttcgatat tttgatcaat aaaacgttct aaaggaataa atggggcttg
    15541 gatattcaaa tcagtaatcc cagtagtagc tatgatctga tattcttttt ggataactgg
    15601 cagttcttct ttcacttcca caatcgacaa agctagaact tctaattctg tttcttgacg
    15661 cttgtttacc gctcgttcga tcaattcttt gattctttgt gctgtacctt cgccagatgc
    15721 gcagatagcc aaaacagctt ttttcttatg aataggtttt gcttcctgaa cagattctac
    15781 agaagatatt tctgcgtaac ctcgaaaatt tttcaatgat tcatgcaaca tatctagatc
    15841 tgtccctaat actgagacct ttcgtacagt ttccaaaact agagaagttg ttaccatatc
    15901 aacagtacgt actggaattc cagtgtctct ttgaatttgc gaattaaacg acgcaaggga
    15961 ccccatatct actaagagta tgaccccgct tccttcatct acttcttgta cattgcgttc
    16021 aattcggtcc aatgctgttt tcggatccat atccaaaggc atgtctactg ctcgtacgtt
    16081 ctctgcatct aacaactgtt ggacaacttg gaccatactg cttgctgtgc tgtttccgtg
    16141 agctgcaacg actacaccga ttctgccgct tgcttgattt gcccgtaacg agacgagtag
    16201 aacggtcaag taatagtctt cactttctgg aatctccact tgaaaatacg tttcgatata
    16261 actacgcact tctttcgcaa cagcatattc atctggaaaa tcaaaagcca tctcacggat
    16321 gttatcattc gtatgccgtt cttctcctaa gttgattttt ttcaaaaaag aactgatatg
    16381 caagctcata gcataaataa agttttgctg aaactcatat ggtaaagatc ttttgaccat
    16441 tacataaatc tgattggtca cttctatgat tttcggatct acaaattctg ctaatttgct
    16501 gtctgcatta aaagaaaaac catggtcttt atagaaagac ttcagatgga tgttgatatc
    16561 tgtagaaatg aaatgattga ttgcttcttg atccaaacca tccgacttca gtaaagctgc
    16621 tttatctccg atgatatcat atagattata aggtaactca taggagtccg attggatttt
    16681 catgatcgta tcatttggtg ctacagttat ctttggctcc aatattttag aaagctctgc
    16741 cattgcactg cggttgcttg ctaattgaat caatccgcta cgaatccctt ctgtcaaatc
    16801 atcgattgtg atcgagattt cgggtgattg catatgattt agaaatcctc gtgcacatac
    16861 taactggata tttgacttca attgtcctat atttccatag gtcacactgc cgatcaatgc
    16921 ttttgctaca tcttctgtca aagaaatctt tcgctgcgtg cggtttgctt cgtgggcaat
    16981 catgattttt actaaatcga ctttctcatt tgcaggacgt ttttcaaaag ccggaagttt
    17041 gatattgatt gggatacgcc gaacaaaagt ttctaataga gaagaacctg ggtcttctgt
    17101 ggtcgcacct acgatccgta catttgcttc atgggatttc gttgtttccc caagacgact
    17161 gtaagttcca tgatccataa agtaaaagat catttcttga ccttcaggcg gcaaccggtg
    17221 aatctcatcc aaaaacagca taccgccatc cgcttgatcg ataatcccca ttttttcttc
    17281 atctgcgcct gtaaaagcac cttttgcgta cccaaataaa tgactcatca aaagctcagg
    17341 gttattggca taatcagcac aattgaaaac aatcagttcc gtttcctcat ctattacatg
    17401 atgcgctttt gcaaaatgat acatcgcgtg tgcaaaataa gtctttcctg atccggtagg
    17461 tcctgtaatc aaacaattta atcctcttgg cggatacaaa atcgcagctt ttgcctgttc
    17521 cactgaattt ttcatgctgc cgttagctcc aatgattttt gcaaagatat ccttagtatc
    17581 tatttggagt gtattttttt cttcagagac cggcgtttct ttgtaactga ctacatgttt
    17641 tgtaactggc tttacgtgcg taacgagtgt ttgatatatg taacgtactg gtcgtccgtc
    17701 tgtctttatc agttttcctt cgcgcactag ctgattcaga tctttactgc tatttgttcg
    17761 ctgaatccct aacacttcag caatttcctg tgttgttact ccttgatcat attctgcctt
    17821 tgttaaatga gcggtttttt ctttgacata catataaatt cggtcaatgc gttttgccaa
    17881 tggtatcgct ccttttctat agactattgt acttaggctt caaaacataa tactatgtat
    17941 ttatttctgt ttcgtcaact atttacctga ttttgttttg ttgataacac gcggatagat
    18001 agaaatgttc aatttaaaag aacgaaaaat cccgatacga tttttataat ggaaacaaga
    18061 aaaagaacac tgtttaccat ataattattt cactgatagc aaacaatata gcttccatca
    18121 attgaaaacg atagttatca aaacatcctg attccaaatt ggcaaaaaaa tattaagtgt
    18181 aaaaagacaa agtaaacttc gtagcctcca ctacaacttt tatttttata aacaaaaaag
    18241 gctgtgacaa aagtcttgtc acaacctctt taatcataag attcaattaa gcttcttgag
    18301 ctactggata aacagacact tgttttttgt tacggccttt acgttcgaaa cgtactacgc
    18361 catcaacttt tgcaaataaa gtgtcgtctc caccgatacc cacgtttaca cctggataga
    18421 tttttgtacc gcgttgacgg taaagaattg atccacctgt aactgtttgt ccatcagcac
    18481 gtttagcacc tagacgtttt gattctgagt cacggccgtt agatgtagaa ccgccacctt
    18541 ttttgtgggc aaataattgt agattcatag ataacaacat ggtctgcacc tcctatttat
    18601 cattaattgt tttaatttgg atatattctg agttttcagc ttctatcgat tgtaatccta
    18661 ataaaagatt ttctaataga atctcagcaa tattgttttg ttcttgtgtc attcctgaag
    18721 tgacttcggt ataaagataa ccgccttctt cttcattcat ttcaacaatc ggctcaaaac
    18781 cggctaatga ggcaatcccg ttgacagtgc tgatcgctaa cgcagaaaca cccgcacaaa
    18841 cgatatcact tccataagga ccagcatcag catgcccggt caaagtgaat gaaacgatct
    18901 gacctgcgtc atttcgctta aatgttcctt tgatcatata taggccttct ttctcaaacg
    18961 ctattaagcg ttgatcgcgt cgataaccac ttttgtatat ggttgacgat gaccttgttt
    19021 acggtgtgaa tgttttttag gtttgtattt gaaagtaacg actttctttt gtttgccgtg
    19081 tttttcaaca gtaccttcaa cagttgcacc tgcaacagtt ggagcgccta cttttgtaga
    19141 ttctccgcct actaagataa cttcgtcaaa aacaactttt tctcctgctt caacgtcaag
    19201 tttttcaaca tagattgctt gtcccacttc aacttttact tgtttaccac cagttttaat
    19261 aattgcgtac atgctataaa gcacctcctt ctgatatact tagactcgcc atccgaagtg
    19321 acagcatatg ctgtgcttat tacttgttct gagcggttgt agctgtggag tccacaatta
    19381 caacagtact agcttaccag aacagcaacc gttagtcaac taaatttaaa atcaaaaagt
    19441 aaaacaaaaa ttcaagcaat acttctgctt ttctatcctc acagttctac caaataaaat
    19501 agtcgcattg tgtttctttt attcttataa taaaagaaaa aggaggagca taaatggaat
    19561 acccaaaaga actgatacaa gaagcttctt taagaatgaa cggccgtcct gtagaaggat
    19621 ttatcgctgg gcaaggacct cttcatccta aactgatgct cgtgggagaa gccccaggga
    19681 aaacagagat tgatacgcat gttcctttta gcggtcaagc tggaaaagaa ttgatgcagg
    19741 cactttcttc aactggctta acacgagaag aggtgtatat caccagtgct gtaaggagcc
    19801 gcccgtatcg cgtgactcat cggatcaata aacggacaca acagtcagaa actgtttatc
    19861 caaatcggac acctactcgt tccgaagtat ttgcgcatgc accaatattg gattacgaac
    19921 tgatgaaggt ccagccaaaa ttaattgcga cacttggaaa cattggtctt caacgtttac
    19981 ttggcaaaga atatacggtt accaaaaatc atggtcaact ctatacaggg cctgtcttac
    20041 aattaacaga acaaaaggat gcctacgaat ggtcagaaaa aaactatcga attcttccac
    20101 tttttcatcc agcagctatt ttctacaatc gctcccttgt acctgttatc catcaagact
    20161 ggcaagtact agcttcactt ttacagcaat attaaaaaga acagacaaga ccataaaatc
    20221 ttcagtttac agtcttgtct gttctttttc attgttttaa tggctaattg cagtatattt
    20281 cgcatgtaaa gctgtcaacg catgagcaac tgtttgatgt tgatgcgtga atgtatgttc
    20341 ccacaagtga gaaagttgtt tttctacttc tggctgcaag gaagtataga taatcgtttg
    20401 tcctctttgc tctgcttttt cttgaaattc atcaaaaact gcacgtgccg tcaaatcaat
    20461 acttggtacg cctcgcatgg agaaaatgac accatcgtga tgatttagtt cttccaattt
    20521 atttttgagt ttttctgaag acataaaaaa cagtggacca ctaatataca ctactgtcca
    20581 gttattcagc ttttccgttt ctggcagccc cattctttgc caatcgatcg tttctaatga
    20641 aatcgtgata gcagcgcttt tgacaacaaa aaatacacag ccgccgatca ccccgatcac
    20701 gatagcaata cttaaatcaa agataactgt acaaatcatc gtaaggataa acaatagaac
    20761 tgctgaccaa tatttattag taaatagctc cttgatcgtt tcccattcat tcatacgcca
    20821 agccgtcaca atcaaaacac ctgcaagtgc tggcattgga aactgtgaca tcactggcgc
    20881 aaaaacaagc atggagagca gtaagacaac tgcatgtatc ataccagcta atctagtttg
    20941 tgcaccagat ttgatggcca cgcttgtacg ggcgatggcc gcagtagcag gaatcccgcc
    21001 aaagaaagga agcaacagat ttccaattcc ttgcgccatt aattcttgat tgctatctaa
    21061 gggcttattt gccattcggc cagccgaagc accacatagc aagctttcaa tcattcctaa
    21121 taaagcaatg ctaatagctg gaactgccac ttgttgcaag gcagaaaagc taaaatctcc
    21181 catattcaaa cgattactac tgatcaatgt ttgtggaatc ttacccacag ttgctactgg
    21241 taaatggaac aatagcataa ctgctgttgc caaaatgata gccagcagcg aactaggcat
    21301 tcgtttcgcc catttttttg gatagataaa catccctaaa atgactagcc ctcccatgat
    21361 caaagaggct gtgtttatcg gaaatcctat cgtttgataa ctacttagtt tttcaataac
    21421 gtttgtacct tccgagtgaa caccaaataa attatcgatc tgacctaatg caataatgat
    21481 agcaatacct gaagtaaacc ccgtaattac gggtgctggg ataaaagaag tcagtgttcc
    21541 taatcggaaa agtcctgctg caagtaaaaa tagacccgct aaaaaagtag caagcaatac
    21601 accttgcatt ccctttgttg ccgcaataga catcaagata gctgccatcg cacctgtcgg
    21661 acctgatatt tgataaaaac cgcctgataa accgccaata acgataccgg caatgatagc
    21721 cgtaatcaaa cctgcggccg catcagctcc gcttgatacc ccaaaagcca aggctaaagg
    21781 caatgctaca gcagctactg tgatcccagc caaaagatct ttctgaaatc ttgaaagatc
    21841 ataacctgaa aattcttttt ttagtgagga aacataattt ttgaacacct atctaactcc
    21901 tttttcatga aattttctaa aataccattc attagaatat catatttcat gagataaagt
    21961 aatatttttc acaaagtttc ctgttagata aattcttatt ttttctatat ttataagaaa
    22021 atagaaagca atgccaacag agctagccca ccttgtttga gtaaaatcca acgatcgcta
    22081 gtcaaactgc catataatgc aacaaaaaaa atagatatta atagcatact tgtgatttct
    22141 ttagatgcgg aagaaagata agctccataa agtagtccca ctcctaatag tccattgtac
    22201 actccttggt tcttaaacaa tgtttgaaca gattttctgc ttaactcttc tttactcatt
    22261 ttaaaaaccc tacttgtcgt gtcagaatca gttctcaccg tttcgatata catgatataa
    22321 aaaaattcaa ttgcaactag agtcactaaa attctagata gtatcgtcat atcttcttcc
    22381 tcttttctga tgagttttat ttttttgctc cttataattg tactcgataa ggatgttttt
    22441 ttattttatt tgcctttgaa aacaaacaat attggcacaa aaaacaaaga aaaaccgtta
    22501 gaaacctcag tttctaacgg ttttttagcg tcacaggagg gattcgaacc cccgaccgtc
    22561 cgcttagaag gcggatgctc tatccagctg agctactatg acaagaatca gcggaacatc
    22621 aataattata gataattaaa ttgcttctgt caatacgaaa tagttattta attagcaaat
    22681 aatgaattat tttggcccaa ctactaaatc aatcttttaa aacgagacat tctccttcag
    22741 ctgttctaag gttatcaatt gaaaacagga gctacattga cttcatgcta ctcctgttta
    22801 tttagatttc ctgtatcctt taattgactt acaaatatag aaacttttac atcaagttcg
    22861 aattgagccc tcgattcaat cgattatcta caggctgcca taatcatgta tcacaactcg
    22921 attgtttctg attcctttgt cattaagatc acaccatcac ctaatggcaa gattgtcgat
    22981 gtcaaatcgg gatgatccat cacaacacga agaaattcat ttaatttacg atggatagct
    23041 cgtttgccac ggggaatctc ttcatctggg agcaagatcg ttcctccttg gaagatgtca
    23101 tctaccatca aaacgccacc ttttcttaac aaacgtaagc attcaggtaa aaattcaata
    23161 tattttgatt ttgcactatc cataaaaata aagtcatacg gaccagacaa ctccggcaag
    23221 atatctgctg cttgaccttc aagcaaagtc actttatctg tcagtcctag acgttcatag
    23281 ttttttttag ctttctcgat catgacatcg aaacgatcaa ttgtcgtaac atgagcgttt
    23341 tctccgattg tcgtggccat gacacttgat gaaaaaccga tagctgctcc gatctccaag
    23401 attcgttcag gctgaagctg tccaagtaaa aatttcaaaa atacaactgt ttcatgggga
    23461 ataactggta cttctgctac atgagcttct tcttccagtt tgcctagctc acccggtaat
    23521 tttttttgct gggtacgcat aaaatccaat agttcttctt tgactacagg acgatgcatc
    23581 atttcattgc gcatactact cctcctcttc acacattata cgaacaactg actaaatcta
    23641 caagctgcat gagtgttttc tcgtgtttca tgttcagaag agtcgttgga tctctggaat
    23701 acatttatgt ttttaacatt cttacaaaaa tttctgtgtt cgtctccgct tctttttcaa
    23761 ctctacaaag gtattccatg ctgtgattcc tgttaggcaa aaagcaatcg taaagaataa
    23821 cccctgaatc catgcaaatg ccgtttttga aaggactagt ccaatatatg gaatctgaaa
    23881 gatgggacta ccgataatat ttctcttttt tactactcca ggctcaatat aagtctttgt
    23941 atcagctttt gtataaaaat cttcgtggtt gttttcaacg gctattacgc gacgcgtcga
    24001 gatttttttt cctgaatttt cataatatgt aataatatct ccgactaaaa tctcatttgt
    24061 atttttcggt cgtacatata tcagatttcc ttgtgcataa tatggactca tttctttgtc
    24121 ctcaataata taaggagtca aaccaaaaat tcggggaacc aagacgacga gaagaataag
    24181 aattaaaaat ataatcaacg ttatagaact tataaaagtt atttttttgt aatcaggtat
    24241 cgttttcaac ttctttcttt attcaatcct tcttaatcat ataataacga taaatttttt
    24301 caaattatag ctgtaaaaat gtttcccttt aat
//
LOCUS       DASJTC010000040        23620 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.040, whole genome shotgun sequence.
ACCESSION   DASJTC010000040 DASJTC010000000
VERSION     DASJTC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.040"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            299..1960
                     /locus_tag="U4B19_001427"
     CDS             299..1960
                     /locus_tag="U4B19_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2077174.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2392)
                     /locus_tag="U4B19_001428"
     CDS             complement(2030..2392)
                     /locus_tag="U4B19_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEN2077175.1"
                     /translation="MKEQSNEKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFL
                     WSLPFLIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLII
                     AGILIALSAIFMLFSLLG"
     gene            2460..3401
                     /locus_tag="U4B19_001429"
     CDS             2460..3401
                     /locus_tag="U4B19_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2077176.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3402..4445
                     /locus_tag="U4B19_001430"
     CDS             3402..4445
                     /locus_tag="U4B19_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2077177.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4451..5521
                     /locus_tag="U4B19_001431"
     CDS             4451..5521
                     /locus_tag="U4B19_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077178.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5511..6464
                     /locus_tag="U4B19_001432"
     CDS             5511..6464
                     /locus_tag="U4B19_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2077179.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6476..7486
                     /locus_tag="U4B19_001433"
     CDS             6476..7486
                     /locus_tag="U4B19_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2077180.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7771..8592)
                     /locus_tag="U4B19_001434"
     CDS             complement(7771..8592)
                     /locus_tag="U4B19_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2077181.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8689..8967)
                     /locus_tag="U4B19_001435"
     CDS             complement(8689..8967)
                     /locus_tag="U4B19_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077182.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9235..11244
                     /gene="metG"
                     /locus_tag="U4B19_001436"
     CDS             9235..11244
                     /gene="metG"
                     /locus_tag="U4B19_001436"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEN2077183.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11723..12310)
                     /locus_tag="U4B19_001437"
     CDS             complement(11723..12310)
                     /locus_tag="U4B19_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077184.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12485..13807
                     /locus_tag="U4B19_001438"
     CDS             12485..13807
                     /locus_tag="U4B19_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2077185.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13962..14738
                     /locus_tag="U4B19_001439"
     CDS             13962..14738
                     /locus_tag="U4B19_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN2077186.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14731..15300
                     /gene="rnmV"
                     /locus_tag="U4B19_001440"
     CDS             14731..15300
                     /gene="rnmV"
                     /locus_tag="U4B19_001440"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEN2077187.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15293..16177
                     /gene="rsmA"
                     /locus_tag="U4B19_001441"
     CDS             15293..16177
                     /gene="rsmA"
                     /locus_tag="U4B19_001441"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEN2077188.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16628..17257
                     /locus_tag="U4B19_001442"
     CDS             16628..17257
                     /locus_tag="U4B19_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077189.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17381..18490)
                     /gene="ugpC"
                     /locus_tag="U4B19_001443"
     CDS             complement(17381..18490)
                     /gene="ugpC"
                     /locus_tag="U4B19_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEN2077190.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18699..19121
                     /gene="mgsA"
                     /locus_tag="U4B19_001444"
     CDS             18699..19121
                     /gene="mgsA"
                     /locus_tag="U4B19_001444"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEN2077191.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19444..19980
                     /locus_tag="U4B19_001445"
     CDS             19444..19980
                     /locus_tag="U4B19_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEN2077192.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20020..21783)
                     /locus_tag="U4B19_001446"
     CDS             complement(20020..21783)
                     /locus_tag="U4B19_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077193.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21786..23501)
                     /locus_tag="U4B19_001447"
     CDS             complement(21786..23501)
                     /locus_tag="U4B19_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077194.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7610 a         4320 c         4795 g         6895 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt tttcatttga ttgctccttt atttttggtt
     2401 cattatctaa taatgtagcg aaacatctgt cagaagtcaa aagctagggg gaaacattag
     2461 tgaatagtta tatcaaatat gtattaaaac gtgtcttttt catgatcatc acgctttggt
     2521 taattgcaac aatcaccttt ttcttgatgc aattgctgcc gggcacgcca tatacgaatc
     2581 aggaacgttt gagtcctgaa acaatcgaga tgctaaataa acaagtcggt ttggataaac
     2641 cagttatcgt acaatatggg atttatcttt ctaatttgct tcaaggagat tttggaattt
     2701 ccttccaatt taaaaaccag ccagtcgcac atttgttagc tggaagaatc ggaccatcct
     2761 tgcagttagg gttacaggca attatctttg gaacagtcct agggacgatt ttaggaacga
     2821 tctctgccat gaagcaaaat acatgggcag atacatcttc tacactcgta gcgatattag
     2881 gtcgttccat tccaaacttt gtatttgctg ttttattaca atatatcttt gcaatcaaat
     2941 tacaagtttt gccaatcgca aaatgggatg gctttgtgta cacaatctta ccaacaattg
     3001 cgttggccat gtcgccgctt gctgactctg ctcggtttat ccgtacggaa atggtggaag
     3061 tactgcatag tgattatgtg gaattagcaa aagcaaaagg attgagccgt tgggaaatcg
     3121 cctttaaaca tggtcttcga aatagtttga ttccattgat gactttatta ggaccgttag
     3181 ctgtagcatt gatgaccgga tcactggtgg tagaaaatat ctttgctatt cctggtatcg
     3241 gggaacaatt cgttaagtca atcacaacaa atgattatcc tacgatcatg gctgtaacga
     3301 tcctttactc atttatgctg atttttgtta ttttgatcgt ggatctattg tacggtctag
     3361 ttgatccaag aattcgtgtt tcagaaggga gccgaggata aatggaaatg ataccaaagc
     3421 gttatgaaac agttgcagat atccctgcgg atgaatttct tccattacaa aaaaataccg
     3481 aagaagaacg tgaacagatt actgcacctt ctttgaattt tatccaagat tcatggcgcc
     3541 gtttaaagaa aaacaaagct gccgttattt caatggtttt actattggtc atcattttga
     3601 tctctgtgat cacaatcttt gtttctcctc atgatccaac tgcccaaaac gtggcttata
     3661 tcaatttgcc tcctcgtatt ccgggaatca atatcgatgg attgaatgga aaagcaatgg
     3721 ttggtggtga acttgttgat aaatatgctc aagcaaacgt cccttcaaac gtaaatttct
     3781 ttttaggaac agacggtttg ggacgtgatg tattaagccg cttatttatg ggaatgagaa
     3841 tctcgttgct gatcgcattt attgctgcat tactagatgt aacgattggt gtagcttacg
     3901 ggttggtttc tggtttgtta ggcggacgtg tcgataacgt gatgcaacgt tttttggaaa
     3961 tcttatcagg aattccaaac ctcgttgtca tgattttgat gttggtcgtt tttgaaccag
     4021 gaattttttc aatcgtggct gctatggcca tcacaaactg gataccaatg gcacgtatcg
     4081 ttcgtgcaca aacaatgaaa ttaaaagatc aagaatatgt tttagctgga ttgacattag
     4141 gtgaatcaag aatgaaaatt gcattcaaac atattttgcc gaatatctct agtgtcatta
     4201 ttattcaaat gatgtttagt attccatctg ctattttctt cgaagccttt ttaagcttta
     4261 tcggtttagg tcttacgcct ccgtcagctt cattaggaac gatgttgagc gaagggtaca
     4321 agacattcct gtatctgcct tatctattat ggattccggc agtaacttta tcggttatca
     4381 tgattggctt taacttactt gctgatggat tgcgtgatgc ctttgatcct aaaatgaaag
     4441 agtgaagacg atgacgaaaa ttttagaagt gaaagactta gaaatctctt ttgatacgta
     4501 tgctggaaag gtacgagcga ttcgcggtgt caattttcat ttgaataaag gagaaacatt
     4561 ggcgatcgtt ggggaatctg gaagtggaaa atcagtgacg accagaagca tcatgcgctt
     4621 gctgtcaagc aacgccaata tagatgctgg tcaaatcctc tttaaaggta acgatatcgt
     4681 tggaaaatca gaaagagaga tgcaaaaaat ccgtggtaaa gaaatagcga tgattttcca
     4741 agatccaatg acctctttag atccgacgat gcccattggg aagcaagtcg ctgagtccct
     4801 tagaaagcat aataaagttt caaaaaaaga gggacaaaaa gctgcattag aattgcttaa
     4861 attagttgga atacccgaag ctgagaaacg tatcaacagt tatccgcatc agttctcagg
     4921 aggtcagcgt caacggatcg tgatcgcgat tgcactggta tgttatccgg aaatcctgat
     4981 tgctgatgaa cctacaacag cgcttgatgt gactattcaa gcacagattt tggagctgct
     5041 gaaagaaatt cagacaaaaa tcgatacatc aattatcttt atcactcatg accttggtgt
     5101 agtagcgaat gtcgctgatc gtgtcgctgt catgtatgga ggaagaatcg ttgaagttgg
     5161 aacatcggaa gaaattttct acaacccgca acatccctat acttggggac tgcttggttc
     5221 aatgccgaca ttagatagtg cgaacgatcg attgtatgct attcctggtt ctccaccgga
     5281 tttattgaac ccgccaaaag gagacgcatt ctatccaaga aatgaattcg cgatgaaaat
     5341 cgatgcagaa caagaaccac cattttttga actatcaaaa acacacaagg cagcaacttg
     5401 gctacttgct ccgcaggcac ccaaagtgac accgccagaa gaaatccaac gtcgctgggc
     5461 tatctttaaa gagaaacaaa aacatactta cggtcaagga ggaattgctg atgccaaata
     5521 aactacttga agttaaagga ttgaaacagt atttcaatgt aggaaaaaag aatgaagtac
     5581 atgctgtaaa tgacatcagc tttcacattt atgaaggaga aacattcggt ttagttggag
     5641 aatctggaag cggaaaatcc actactggcc gtacgatcat tcgcttgaat gaacctactg
     5701 atggtgagat attattcgac ggtcaagata taacgaagat caaagataaa aaaggtttga
     5761 cgaaattccg gcatgatgtt cagatgatct ttcaagatcc atacgcttcg ttgaatcctc
     5821 gtatgaaagt cagagatatc attgccgaag gaattgatgt taatggacta gctaaatcat
     5881 ctgaagagcg tgcaaaaaaa gtcgatgatc tattgagaac tgttggattg aatccaagcc
     5941 acggtactcg ttatcctcat gaattttcag gtgggcaacg ccagcgtatt ggtattgcac
     6001 gagcattggc agtaaaaccg cgttttatca tttgtgatga accaatttct gcactagatg
     6061 tgtcgatcca agcccaagtg gtcaatttgt tgcaagatct ccaaaaagaa catcaattga
     6121 cctatctatt tattgcacat gatttgtcga tggttaaaca tattagtgac cgaatcggtg
     6181 taatgcataa cggcttgtta ttagagatgg gaacaagtga tgaaatctat aatcacggtg
     6241 ttcatcctta tacagaaagt ttgttatctg ctattcctct accagatcca gaccatgaga
     6301 gaaaacgcag aagaatcaaa tatcaacctg aaccagatga cggacaagta cgtcaacttc
     6361 gtgaaatcgc accagaacat tttgtctatg caactgagca ggaagtccca tattacgcaa
     6421 aaaaactaaa acgacaaaag gaatctcttc tggttgctaa ctaggaggga catagatgct
     6481 aaaagctttt aaattccgta tgtatccgac agaagaacaa aaacagcaat tgattcgtac
     6541 ttttggatgt gctcgattta cctataacca tttgctgaaa aagcggcaga aaagttggca
     6601 acaaacagga gtagccaatt tttcgctcac acccgctaca ttaaaaaaag aatacccatt
     6661 cttaaaagaa gtagacagtc ttgctttagc aaatgcacag cttaatttgg atcgggcttt
     6721 tcgcaattat ttcaagggac gtgccagctt cccaaagctt aaaacgaaaa aaagtatgtg
     6781 gcaatcttat acgaccaaca accaaacccg tacgatctat ctggaaaatg gctacttaaa
     6841 gttaccaaaa caaaaagaac taatcaagat aaatcagcat cggccagttg aaggatcgat
     6901 tcgctccgct acgatttctg ctcgatataa tgaagaattt tatgtcgcac tactttgtga
     6961 tgtttcgcca gtaaaaaaag aatctttagc taaatggatc gggatcgcct atcatccgaa
     7021 gacattgatc caaacatctc gacctctaga agtaacttta ccaaaatttc accagacaga
     7081 agaaaagctg caacatgcac agcggaaatt gaatgtgaaa gtccgctccg ctcaccatag
     7141 aaagatcagg ttagatcagg caagcaatta tcagaaacaa aagagaaaag taatggatct
     7201 ttatctgaaa caaaagaacc agcgagaaga ttatcttgaa caattatctg ggaaattggt
     7261 caaacaatat gactacttat ttgtagaatc ttttccaaaa gaagaggcac atgctgattt
     7321 ttctattcac gactggcata aattaataac aaaattgcgt tataagtccc aatggtataa
     7381 taaaaaattt cttttgatca ataccgacgg agcagaagaa agcaattccg taagaaagag
     7441 ccaagtagta gaaaaaatgg gcagacattc ggttatcaag ggataaaaaa tgaatgaaac
     7501 tgataggagc gttagggtta gccttgttaa taaaaagtct taatgacttt tgtttctagg
     7561 aatttttctt ttgaagaaaa gctcactaat tatgacaaac caataaaaca atagcagaaa
     7621 aagttcgtac gagctttttc tgctattgtt aattttttat tcagaaagtt tacgaataca
     7681 tcaaaattct gaaacaaaaa acgtaactca caaagagttg tttttgcgtt ttttcttgaa
     7741 tgattaggtc tgaatctttt gctcacaacc ttattcatgg tgccccggct tgagctgata
     7801 gatttttgaa ggacggccga ttcctacagg acgttcaccg atctcatcga aatattggag
     7861 catagctttc ttaaaattgg aatgatcgat cgttttataa tcgatcccta agaatttagc
     7921 aaatactttt cgagcttccg taatcgtgaa atctttacct aaaacttgta gaacttgcgg
     7981 ctcgtgttcc attttattga ctacacggtt gaatgctttc aagatgattt gactatggtc
     8041 aaaagccaaa gtatctttgc cagaagactc tccagtgact aaatcaagcg agatagccac
     8101 ttctccgcta gataaatgga tcttgttact atgacgttct agtgtgaacc agcgtacttc
     8161 cttggcatca tctccggcga ttagcggttc ttctccaata aaggctaagt agctgactgt
     8221 gacgacccag cctcgcggat ctcgattagg cgtactgaat gtatgcagtt gctcaatatt
     8281 ttcttttgaa ataaccacac cagtttcttc ctttgtttct cttaagacgc tttctccagt
     8341 tgactcatcc ttttggacaa agccaccagg caatgcccat gaatttcgat aaggatgccc
     8401 ttttctttgg atcaacaaaa ctttcagctg atcttcttct ttgttatagc acatcagtac
     8461 gatatcgaca gtcaaagagg gcttgtcata ttctggacgt tcctgttttt gataccagtt
     8521 aagaaattct tcttcacttg ctttatgttc ataatataat ttttcttctt ctttagaagc
     8581 aaaacggttc atctgaccac ctccattgac agtaaaagta ctttttacct tattgtagca
     8641 gactctgaaa aaaaaccaag gatttacttg aaaaatctag gttatcattt aaatatgaaa
     8701 aataaatgga agtatgagat tgaataaaaa gaaaagcaga taaaacggca atactttata
     8761 ccgctcttca cgaatggcaa agaagccaca aataacgata tatacgtcaa tgataaaacg
     8821 caaaatccaa gctgctgtca taaaactgtt caaatgccca tggaggttaa aataaccata
     8881 catacgaata taaaaatagc tgaatattaa atgtgctaat gaaagaaaag caatactaag
     8941 tgtaaaaaaa agatgtttct tctgcatgat tgttccagtc ccttactaag atagatagtc
     9001 tttattatac cacgtgtgag gggagaaata aaaagccgtt ttttagtaga tatgaaacaa
     9061 aacaaaaacc cgcatttatt caacctattc atccttttaa aggatagatt gaacgtagat
     9121 agaagtcgtt tatccagtct ttttcatttt gaaaaaaagc taacagcatg ctttttttat
     9181 ggtatactag caaggaaacg aattcgcatc atgcgataaa ggaggaagag ccccgtggca
     9241 gaaaaggaaa ctttttatat cactacgcca atctattatc caagcggcaa acttcatatc
     9301 ggcaactcgt atactacgat tgcatgtgac gcaattgctc gttataaacg gttgatgggc
     9361 tttgatgtgt tttatctcac tggagtggat gaacatggtc aaaaaatcga aacaaaagca
     9421 gaagaactag gtgtaaaacc gcaagaatat gtagataaaa tggcggctga tgtcaagaaa
     9481 ttatggaaaa cgcttgatat cagttatgat aaattcattc gcacgacaga tgattaccat
     9541 aaagcagctg ttcaaaaaat atttgatcgt ttattagaac agggagatat ttacttaggt
     9601 gaatatgaag gctggtactc tgtttctgac gaagaattct ttacagaaac acaacttgca
     9661 gaagtgtaca aagatgacga aggtaacgtg atcggtggta aagcgccaag tggacacgaa
     9721 gtagaattgg taaaagaaga atcatacttc ttccgcatga gcaaatacgc agaccgtcta
     9781 ttggaatact atgaagaaca tcccgaattc atccagccag aatctcgtaa aaacgaaatg
     9841 atcaacaact ttatcaaacc aggattagag gatcttgcag tttcacgaac aacttttact
     9901 tggggagtac cagtcaagag tgatccgaaa catgtggtgt atgtatggat cgatgcatta
     9961 tccaattata ttactgcact aggatatggc tccgaagatg aaagtctatt cgaaaaatac
    10021 tggcctgcag atgttcagat ggtcggtaaa gaaatcgttc gctttcatac gatctattgg
    10081 ccaatcatgc tgatggccct tgatttgcct ctacctaaaa aagtatttgg gcatggctgg
    10141 ctattgatga aagacggaaa aatgtctaaa tcaaaaggaa atgtcgttta tcctgaaatg
    10201 cttgtggaac gttatggttt agatgcactc cgttattatt tgcttcgtgc tgttccgttt
    10261 gggtcagatg gtgtctttac gccagaagat tttgtttccc gtgtcaatta tgatctggca
    10321 aatgatctag ggaacttatt gaatcgaacg attgctatga tcaacaaata ttgtgatgga
    10381 ctcgttcctg actatgcctc acttgtcact ccatttgata gtgaactgtc tacgacggct
    10441 gctaacgttg tcggacgtta ccatgatgca atggaaaaaa tggaattcaa tacagcattg
    10501 gctgaaatct gggtgctgat ttctcgtgca aacaaataca ttgatgaaac agaaccatgg
    10561 gtattagcaa aagacgaaga aaagaaagct gaattgaata gtgtcatgat ccatttagct
    10621 gaatctttgc gtatcgcagc gatattactt cagccaatca tgacagaaac gccagagaag
    10681 atcttcaatc aactaggatt ggactctgaa acaatgaatc ttgaaggatt acactttggc
    10741 gaatttcctt caggaacaaa agtagttgca aaaggtacac caatcttccc acgtttagat
    10801 atggaagaag aagttgcttt tattcaagaa aaaatgtctg aaggtactca aacaaacgaa
    10861 gatacagtca aatgggatcc agaagaaaca gaacttgttt caacgaaaga aaaacaaatc
    10921 aaatttgatg tatttgaaaa agtagaacta aaagtagcag aagtcatcaa ctgtcaaaaa
    10981 gtagaagggg cagataagtt gctgcaattc cgactagatg ctggagacag ccaagaccgt
    11041 caaatcttgt caggaatcgc tgaattttat cctgatccaa gcgaattgat cgggaaaaaa
    11101 gtcgtcattg tagcaaatct gaaaccacga aagatgcgtg gacaaatcag tcaaggaatg
    11161 atcctttctg cagaagcacc agatggctct ttacaagtaa tcgaagcacc aaaatccatg
    11221 ccaaatggct cagaaatcgc atagaaattc aaagggggta tgacactagt tgtgtcatac
    11281 cccctttttc cgaaatttga gaagcaattt tcgtaaattc cttcttatta cttgaagcca
    11341 atcatataat acagaaacag agaactatcc aaaataaatc ctagaaaaag aagtacgagc
    11401 ttcgctgatt tttgttaatt tagaataata ctaataggaa tcgtaaaaac agaaaaaaag
    11461 cataatgata aagggtcggg cagattttaa ttgattcatc tggtactctt ttttattgtc
    11521 cttacattca taaagtttga atgcaatgac tttattacat ctatatttat atctaacagt
    11581 agcgttgatg tgttaattat aagttctatc actttatgtt caattagttt gtgctcttat
    11641 atgtgtgatt gaaaatatag ataaaaaaac aatcatcggc tttgacttcg atgattgttt
    11701 tctttatttt gtaaaaatcc tatcacttga ttttatggta accttttatt tttgtcttca
    11761 aacggcttgg taagagagca gtaagcagtg caaatgggat cgaaaagaca taccagaaaa
    11821 tcgtacgtcc gataccataa tgttgataga gctttgtcaa catcacgtgt aacaagatca
    11881 accctaaaag caaggagcca gctataaacc aatgattatc ccaatgcatg acttcactaa
    11941 gtgccatcac tccaaaagga tagaaataaa aatagatccc gactaaggtc atccgccagt
    12001 tctttgtaaa tgttccgata ttcaataaaa agaaaatcgc tgaaagcaaa ataccgatag
    12061 gcggaaaaag cccaaccaaa aaggaataca acatccccca caagatcatc acaggacctt
    12121 tgatcaatgc aacgattgcg atgaacaaag tcagcaatat tagtttttgt gataacgttg
    12181 caacagtcat taatccaaat aaaattaatg tgaaccaatg ccaatgggac tgctgttgtt
    12241 ctttacggga aaaatcgacc cggttggata aataatctgc taatttcgga taagaacgct
    12301 taaaatccat aaactgttcc tccttcgtgg aaaaggtagg tttagtatac gaaaaataaa
    12361 aaagaaatac atccgtctta agagggagtt tttcactgaa agtggagaaa aaagaatggt
    12421 aaattaaaat attctcagtt atactgaaaa ttataccaaa tttcaaagaa gaaagaggat
    12481 gggaatgaaa ggcaaactaa aacgagaaat caatctgttt ggcgcattag ctaccgttat
    12541 gggaaccgtg atcggtgcag gcgtcttctt caaaactgct gctgttactg ctagtactca
    12601 gtctacaagt ttgacattgc tagcttggct tttgggtggt ttcttaacga tctgtgctgg
    12661 attgacagtg gctgaattag ctacagcaat cccggaaact ggtggagctg taaaatatat
    12721 tgaagctgcg tatggaaaat tgcccagctt tttattagga tgggcgcaaa gcttgattta
    12781 ctttcctgct aatattgctg cattatcgat tatctttgcg acacaattga cgaacttatt
    12841 acaattatct accgattatc ttttgttgat tgcagtgatc accgctgttt ctgtgacagg
    12901 gttgaatttg ttagggacaa aagtcggtgc ttctgtacaa tcagtgacgt tgattgttaa
    12961 attaatacca attgctgtga ttgtcatatg gggattattg accccaggac aaggaactgt
    13021 ccagttattt ccaatcgaag ctggaaaaga tgttactttt gtcgaaggat tgagcagtgc
    13081 gttgttagca acgttgtttg cttatgacgg ctggttaggt gtcggtgcaa tggcagggga
    13141 aatgaaacgg ccggaaaaag atttgcctaa agcaattatt cttggtttga gttttgtaac
    13201 agtcgtttat ctattgatca attttgtttt tttgaaaact ttgccgattg atcatcttgc
    13261 cggtaatttg aatgcagctt ctgaagcatc agacgtcatt tttggaggaa tcggtggaaa
    13321 gctggtcact attgggatct tgatctcggt atacggtgca ttgaacggtt atacactaac
    13381 tgggatccgc gtgccttatg cgatggcttt agaagacgag ctacctttca gtaaacagct
    13441 gacaaagctt tcaaagaagt ttacggttcc ttatgttcct gcggttttcc agttagcagt
    13501 agcttgtatt atgatgagcc ttggttcttt cgacttttta acagacatgt tgatttttgt
    13561 gatgtggcta ttcagtttac tgatttttat cggtgtcttt attttaagaa aaaaagcgcc
    13621 ggaattgcca cgtccatata aagtaccact ttatccaatc gttccgatta ttgcgattct
    13681 cggtgctatt ttcattttag gtatgacgat gatgacgcaa acgaaattag cattgatcgg
    13741 tataggtgtt accttgatag gtatcccagt ctattatcaa aaaaagaaaa aacgctcaga
    13801 agagtaaaat atttcaacaa cagacagaaa aagtacgaac taggtcctct agatcttcgt
    13861 gctttttcta tttcaataaa acaagttctt ctattttgat ttttgctact tctgcaagta
    13921 gtgcttatgt gctaaagtaa gaatgaaaag gagagataaa catgatattt gattctcata
    13981 cccatttaaa tgctgaacag ttcaatgaag atattccaga aacgattcag cgagcacaag
    14041 aaatgggggt aacgaaaatg gcggtcgtgg gcttcgatac gccaacgatt gaaaagtctt
    14101 tgcaattaag ccatgactat ccaaatatat acagtattat cggctggcat ccgacagagg
    14161 ctggcagtta tacgaaagaa atcgaaaata aattacaaga acaattgact ttaccaaagg
    14221 ttgtagcatt aggggaaatt ggcttggatt atcattggat ggaagacccg aaagaagtcc
    14281 aagataaagt atttcgcaga cagattgcta tcgcaaaaga aatgaaccta ccaatcagta
    14341 ttcatactag agaagcactt gaagacacgt atcaaatcct aaaagaagaa gatgttcgag
    14401 atatcggtgg aatcatgcat agtttcagtg gtgattatga atgggcaaaa cgatttcttg
    14461 acttagggat gcatatttca tttagcggtg tcgtcacttt caaaaaagca ttagacgtcc
    14521 aagaggctgc agcaaatgta ccaatcgaac gattacttgt ggaaacagat gccccttatc
    14581 tggcaccagt tccttttaga ggaaagcgaa atgaaccagg ctatactcgt tatacagtag
    14641 aaaagattgc tgaacttcgg aatcttccga cagaagaagt ggctgcccaa acatggacaa
    14701 atgcgcaccg attatttggt ctaagcgaac atggataaga tgaagatcca agagattata
    14761 gtcgttgaag gaaaagacga tacaagaagg ctgcgggaag tagttgatgc agatacgata
    14821 gaaacaatag ggtctgcaat caatgaagag atcctgatgc aaattgaaca tgcccaagag
    14881 actcgtggag tgatcgtttt taccgatcct gatttttcag gagaaaaaat aagaaaaatc
    14941 attatggaag ctgttcctca ggcaaaacat gcttttttgc cgcgcacaca agctaaacca
    15001 aagaaaaagg gaagccttgg tgtcgagcat gcaagtgatg ctgctatttt agaagcattg
    15061 aaaaaggtcg tgacgccaga catttcccaa acggctgttt ctgaaatcac tagacaagac
    15121 cttttagact atggtttaat agctggggca caagcaaaag aaaaacgaga aaaactaggt
    15181 gacgagttgc gcatcggtta cacgaacggc aaacaattgg aaaaacgttt gaagatgttt
    15241 cgtattacgc cgaaagaact cgcccaagca atgaaaatag tggaggaaca acatgagtga
    15301 atatcgtgaa attgcgacac cctctagaac aaaagagatt ttattgaaac atggtttttc
    15361 atttaaaaaa agtttaggac aaaattttct gactgaacca aatattttaa ggaaaatcgt
    15421 agaaactgcg caaatagatg aacatacgaa cgtgatcgaa gttggaccag gaatcggtgc
    15481 attgacggaa caattagcca agcatgcaaa acaagtcgta gcatttgaga tcgatgatcg
    15541 tttgatccct gtattggctg atacgatgca accctatgac aatgtgacta ttgtacatca
    15601 agatatttta aagaccgatt tgtcaacagc tgttagagaa acatttcatg aggagcttcc
    15661 gttaaaagtc gtagcgaatt taccttacta tattacgaca ccgatcatga tgcattttct
    15721 tgaatcagat ttggttgtgg atgaactagt cgtcatgatg caaaaagaag tagctgatcg
    15781 tatctctgct gaaccaggaa cgaaagctta tggttcatta tcgattgctg ttcaatatta
    15841 tatggaagcc agtttagcat tcatcgtacc aaaaacagta tttgtccctc agccaaatgt
    15901 agattcagcg attttgaaac tgacaagaag agatataccg gcagtcgaag tgacagacga
    15961 aaaagcattt ttccgattga caaaagcggc atttcagcaa agaagaaaaa cgctttggaa
    16021 taacttgcag cattcgtatg gcaaagatga ccagacaaaa gcatggttag caaaaagctt
    16081 ggaaacagct ggaatcgatc caaaacgtcg tggggagact ttgtcattac aagaattcgc
    16141 cgcattaagc aatgcgatgt cagaaaatat gcagtaaaaa aagaggttgt gacagaagtt
    16201 gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatgt
    16261 ttttcgtttg tttgggctta ttaccgcaga tgcaagcttt tgaacaccgt ttaagaaaga
    16321 ggttgtgggc ctgccgttta actctaagtc acaaggaaca atttcaaaaa acagcttctc
    16381 atgtttttga gttagttggt cttattactt gatgcaagct tttgaccact attatgccga
    16441 acaaggagat gtgacacaaa ttttgtcaca tctcctattt ttgtttcttg atcaaaaaat
    16501 tcttatactt gatactcaca ctcattttgt atggatttag aaaagaatat gtgtcttgac
    16561 agttattagt aagtgagtta tactaagtcg acatttatgt gtcaagacat attaggagag
    16621 agaaaaaatg agaaaaaatt cagttcgaaa actaacgatt tctgctttat tgattgggat
    16681 gggggtcatt atcccaatgg tcatgcctaa aatcatgatt ccaccagctt cctttacttt
    16741 ggcgtctcat gtgccattat ttattgcgat gttttttaca ccaggagttg cagtggctgt
    16801 tgcgctaggt acgacatttg gtttcttttt aacaacgccg gttatcatcg cattgcgagc
    16861 attgtcacat cttgtgtttg cgttgattgg ggcttggtac ttgcaaaagc atccgagtat
    16921 cgttttaaaa aatggacagt ttacttttgc taattggcgt ttccaagggt tcaattttgt
    16981 gatcggtgtg attcattctt tagcagaaat gctagtagtc agcatctttt actttattgg
    17041 taatatgcct gaaacatatt attctcaagg atatttttat acagtattca tcttgatggg
    17101 gattggtggg cttatccaca gtttagttga ttataatatt gcttatttta tagcaggaac
    17161 actcagcaaa tattttgata tccccgtatt tacggcagca aaaaaagaaa atcggaaaga
    17221 gtcgattcaa gggtacgcga agaaaacgac atattagaca tgatctctca aaaaaaaaac
    17281 tctcatctag taatggataa taaatcctct aagatgagag ttttttgtcc taaaaagagc
    17341 tgcaccaaca tcttctggca cagctctaac agggggcaat ttatggaagt ttgatgactt
    17401 gttctgtttc tttatcaaag aagtggcctt tgctgatgtt gaaggcaagg tcgactattt
    17461 cgcctggttt atggaaatct cttgcatcta cttttgagat gaactcagta gaaccagttt
    17521 ttgtgtaaag catcgtttca gctccaagta attcggaaac gacaacttct gatttcacta
    17581 cggcttcagg tgaagcttca agggctactt gttcactgtg gatgtcttct ggacgaatac
    17641 cgaagataac ttcttttcct tcataacctt tttcaactaa tactttgttt tttccttcag
    17701 gaatacgaag cttaagccca tgtccatctg tgataacacc atttttcaat gtcacattaa
    17761 agaagttcat ggcaggagat ccaataaagc cggcaacgaa catattgacg ggtgtatcat
    17821 aaacttcttt cggtgtaccg atttgttgga taaatccgtc tttcatgata acgattcggt
    17881 cagccatagt cattgcttct gtctgatcgt gagtaacata gatggtcgtt gtttcaagac
    17941 gctggtgaag tttggcaatc tcagcacgca tggctacacg taatttagca tctaaatttg
    18001 ataatggctc atccattaag aagacttttg catcccgaac gattgcccgt cccaatgcca
    18061 cacgttggcg ttgtccacca gataaagcag ctggttttcg tttcaagtat tctgtcagac
    18121 ctaagatctc tgctgcattg tccacacgtt ttttgatgtc tgctttatca tatttacgta
    18181 atttcaagcc aaaagccata ttatcaaaaa cagtcatatg aggatacaat gcgtagtttt
    18241 ggaaaaccat ggcaatatca cggtctttag gtgccacgtc attcatgact tttccgccga
    18301 tatttagttc tccttcagag atatcttcca gtcccgcgat cattcgcaaa gtagttgact
    18361 ttccacaacc ggaaggacca acaaaaacga taaattcacg gtctttgatt gatagattaa
    18421 aatcagttac agaataattt tctgcattgt catatttttt atatacgttt ttcagagcca
    18481 tttctaccat aatacgttcc acactttctt ttttagtttt attaactatt taaagtataa
    18541 atgaaagcgt taactaactt caatgtcatg ttgaacaaga attttttctt ttttttgtca
    18601 atgtgctagt tttaagcttt aataaatatc cataagaaat aaaaactgat acactggagt
    18661 aaataagtat gttaacaata tcaaaaggag tgtctcttat gaaaatcgct ttgatcgctc
    18721 atgaccgaaa aaaacctatg atggtgaaat tagcaacagc gtataaaaca atattggaaa
    18781 aacatgaact ttttgctacc gggacgactg ggcagaggat cacggaagca accggcttgc
    18841 cgatccatcg gtttaaatcc ggaccgttag gcggggatca gcaaattgga gccatgattt
    18901 cggaggatca gttagatttg gtgatttttc ttcgagaccc gctggctgca cagccccatg
    18961 aaccagatgt aacagcactt atccgtttat ctgatgtcta tgagattccg ttagctacga
    19021 atatcggaac agctgaggtg ctactaagag gactggaagc cggcttccta gattttcgaa
    19081 gaatcgtcca tgaaatagac aagcatccac tttcattttg atggataagc gtatggaaaa
    19141 ataacgttta atgttcgcct atatgtagct ataaaagcta aacaaactga taatatgcga
    19201 tataatacga acgtattgga taatataaac aataatatta gtttttcgta tttgcagtaa
    19261 atgtgaaaat atgctatagt ttctgtggga aaagatccca aaaataatac agagtagaaa
    19321 ataaagcgtt aagtgctaag aggatgggat gttgcctctt ggacgaagga cgtaatgttc
    19381 gcggttttat tttcgcattc gctgcatgat acgatgtagc agctcttaaa ggagatgcta
    19441 aaaatgaaga aacaccggtt agacgcacgt atgattgcta ttatgggctt attgattgca
    19501 ttgatggtca ctttgtcccg actggtagca atcgagaccc catttattaa aatcagtgtc
    19561 acatttattc cacaagtgat catgggcatt ctttttggtc ctttttggtc agggataggc
    19621 gcagttttag ctgatttagt aggaatggcc ctcttctcga aatctgcatt ctttattgga
    19681 tttacattga atgcatttat tgaaggagca atctacggat tctttttcta tcgaaaagag
    19741 atcacttgga aaaatgccat tctggctacg cttagtgtca cgctgatcat caacttattt
    19801 ttgacaccat tatggcttgc gttgatgtac catgtgccat tgttcagctg ggtcgtttgg
    19861 gcgccccgcc tgttaaaaac agtaatatgg ttaccgattc aatcaattgc catttattac
    19921 gtaggacgta gtattcctta caaaaaaatt ttaagaagtt tagcgatcca cgctaaataa
    19981 aaaacaagca gtcttcccat cccagaagac tgcttgtttt tattgtgctg tgaattgtgc
    20041 ttgatagagc cgtcggtaat aaccatcttt ttgaagcagc tcatcatggg tgcccatttc
    20101 gacaatagct ccctgatcca tcactaatat ctggtccgca tttttgattg tggacaggcg
    20161 atgggcaatc acaaaactgg tttttccttc catcattgct aagaaggctt gttggatttt
    20221 tttctctgtc aatgtatcga ctgagctggt agcttcatct aagatcaaca tagggggatt
    20281 actgatcatc gttcgagcaa tcgtcaacag ttggcgttgt ccatctgata tctttaaccc
    20341 ttgaccgcca atcttagtat ctaatttttc cggcaaacgt tctacaaatt catacatata
    20401 agaggctttt agggcatcat tgatttctgc gtcacttgct tccggattcc caaaacgaag
    20461 attttctcgc aatgtactgt caaatagcca agtatcttgt aacaccattc caaaactttt
    20521 acgtaatgta tcacgttgga tagagcggat gtcatggtca tcgactgtaa ttgttccttt
    20581 tgttgtgtca taaaaacgca tcagcaaatt aacaagtgtg gattttccgg cccctgtttt
    20641 tcctactata gcaatggttt gacctggata agcagtaaaa ttgaaatctt cgatcaaacg
    20701 ctgcttgggg tcataagaaa aagaaacatg atcaaaagag acttttcctt tgatattttt
    20761 gggttccaag gcataggcat ctggcaaatc aggcttttct tctggctgat cgatcaaatc
    20821 aaatgctcgt tctagaccag aaaaagcggt ttgcagttgt gtcgtgattc cagaaagttc
    20881 gataaatggt ttagaaaact gacttgagta gatcgtaaag ctggaaatca caccaacagt
    20941 gattgacgaa ccactcttca atgccaatag gccgccaact aaaccaatcg ataaataagc
    21001 aaggtgatct acaaaacgag aagacgggtt tgtcaaggaa gaagaaaatt gtgcccgttg
    21061 tcctttttca tatagatcag catttagcag ttcaaattgc tgttgactag cttcttcttg
    21121 ctgaaaagct ttcacgattt tctggttacc gattcgttcg gtcacaaagc ccgagatatc
    21181 cccaataatc ctttgctgtc tatcgaaatt tgtttgtgaa gcacgagcaa cgatccaact
    21241 cacaaggaaa ataattggtg tagaacaaag aacgaccaat gtcagaagtg gacttagacg
    21301 catcataaaa ataaacgaaa gaatgatgat tgccactcca gaaaataatt gattgaaggc
    21361 cgcagaaatt gcaatggaaa tattgtccat atcatttgta aatcgactga cgatatttcc
    21421 gtgggagttt tgatcataat aacgaagtgg caatcgattc agatgagcaa atgcatcttt
    21481 tcttaatttt gcaacggata agtaagctaa ccgattgctt aacacttgga tgagccattg
    21541 actgactacg ttgattaaaa cgataccacc gaatagaata agtaaatgga acagtcgatt
    21601 gaagcggacc tgtccttctc caatcatttg atcgatactt tgaccaattt gtaacgtcat
    21661 cacaactgtt gcaatcccat tgatgattcc tagtatcaca gctagccaaa tgtcttttgc
    21721 ataggcagta aggtaaggaa tgaatcggcg gagcgtctgc aaggataatt tttttacttt
    21781 cataactatt cttcctcctg tgatgcgacg atttcctgat attctgctga ttgagcaagt
    21841 aattcttcat gggtaccctg agcagcaatc ttaccttgat ccatcactaa gatatgatgt
    21901 gcttcttgaa tagagcggac acgttgggag ataataatca ctgtactgcc aagataagtc
    21961 tgtagcgcct tacgcaaatc caagtctgtt tgataatcca acgcacttaa agagtcatct
    22021 aaaatcagta atgctggctt ggcaatcaaa gctcttgcaa tcgtcagtcg ctgtttttgt
    22081 ccgccagaga aatttttccc attttccatg acttttgtat ctaaaccatt tggaagctga
    22141 cggacaaatt cttctgcctg agcgattttc agagcttccc aacattcttt ttctgtggcg
    22201 tctttcttac cccattgaag gttttcacga atagtcccgg taaatagtac agaagtttgg
    22261 ggaacaagag caatttgctg acggagatga tccagctgcc aagaacgaac atccattccg
    22321 tccattataa ctgtaccgga actgacatca tagaaacgcg gaatcaattg tgtcaaagtc
    22381 gttttcccgc ttccggttgg accaatgatt ccaaggactg tatttcgctt cagctgaaat
    22441 gtaatatttt ggagagaaag accagaatca ggagtataac ggaagtcaac atggtcaaaa
    22501 gcgataattg cttcagaaga aaaatcggga gtagtactga cctcagcttc aagaatcgaa
    22561 ttttctgtgt ctagcacctc tttcacccga tttcctgaag cttcggcacg tgtgaagatc
    22621 acgacgaggt ttgaaacaac gatcagtgcc aaaagcattt gattcatata attgatcaaa
    22681 gcaagaactt gtccttgttg caagctacca atatttactt tgatcccgcc aactgtcagc
    22741 aaacagatga tcccgacatt catgatcaag gttgtagcag gtgataatag ggcagagata
    22801 ttggaaacac gtaaataatt gttcgctaga tcatttgtgc ttttatcgaa cttctctgtt
    22861 tctttttttg tctgcgcaaa tgcgcggatg acacgaacac cgctaagatt ctgactaacg
    22921 tgttcattga gctgatctag tttttcttgc actttttggt agagaggaac ggtttttttg
    22981 atgataaaaa ataaaatgat tgagaaaata ggcaataatg ctaagaaaaa cagacctact
    23041 tcccagtcga tcacaaaagc catgaccact gacccgatac ttaaaaacgg tgctctgatc
    23101 aataaacgaa tgaccattgc taaagctagc tgaagctgat tgatgtcgtt tgtcattcgg
    23161 gtgatcaagg tatctgttcc aaaatgattc aactcgttgt gagagaaagt attgattttt
    23221 ttgattagct gattacgtaa ctctgtacca aatccttgtg aagctactga agcatagtat
    23281 tggcaaatga tagcacaaat cagaccaatc acagacatag cgaccatcca taagcccatt
    23341 tcaatgattt ttcctcggtc attcattttt agtccattat cgatcaagga agccatcatt
    23401 aaaggcagga caagttcgaa gacagcttca agaaatttga agaacgggcc caaaatgatt
    23461 tgcttacgat agtcttttgc ataacgtaat aactgaatca tatgattcct cctattattt
    23521 atttgtccac aattgaaaaa acaagtacta ccacattgta gcttaggaaa tttagaatga
    23581 aaagagaaca agccttttca taaatgaaag ttttattatg
//
LOCUS       DASJTC010000041        23481 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.041, whole genome shotgun sequence.
ACCESSION   DASJTC010000041 DASJTC010000000
VERSION     DASJTC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.041"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(317..1813)
                     /gene="lysS"
                     /locus_tag="U4B19_001448"
     CDS             complement(317..1813)
                     /gene="lysS"
                     /locus_tag="U4B19_001448"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEN2077195.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1924..2928)
                     /gene="dusB"
                     /locus_tag="U4B19_001449"
     CDS             complement(1924..2928)
                     /gene="dusB"
                     /locus_tag="U4B19_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEN2077196.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2929..3816)
                     /gene="hslO"
                     /locus_tag="U4B19_001450"
     CDS             complement(2929..3816)
                     /gene="hslO"
                     /locus_tag="U4B19_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEN2077197.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4033..6144)
                     /gene="ftsH"
                     /locus_tag="U4B19_001451"
     CDS             complement(4033..6144)
                     /gene="ftsH"
                     /locus_tag="U4B19_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEN2077198.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6234..6779)
                     /gene="hpt"
                     /locus_tag="U4B19_001452"
     CDS             complement(6234..6779)
                     /gene="hpt"
                     /locus_tag="U4B19_001452"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEN2077199.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6779..8224)
                     /gene="tilS"
                     /locus_tag="U4B19_001453"
     CDS             complement(6779..8224)
                     /gene="tilS"
                     /locus_tag="U4B19_001453"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEN2077200.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8344..8814)
                     /locus_tag="U4B19_001454"
     CDS             complement(8344..8814)
                     /locus_tag="U4B19_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEN2077201.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8860..9285)
                     /locus_tag="U4B19_001455"
     CDS             complement(8860..9285)
                     /locus_tag="U4B19_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEN2077202.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9352..9621)
                     /locus_tag="U4B19_001456"
     CDS             complement(9352..9621)
                     /locus_tag="U4B19_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEN2077203.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9632..11227)
                     /locus_tag="U4B19_001457"
     CDS             complement(9632..11227)
                     /locus_tag="U4B19_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2077204.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11242..14763)
                     /gene="mfd"
                     /locus_tag="U4B19_001458"
     CDS             complement(11242..14763)
                     /gene="mfd"
                     /locus_tag="U4B19_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEN2077205.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14780..15340)
                     /locus_tag="U4B19_001459"
     CDS             complement(14780..15340)
                     /locus_tag="U4B19_001459"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEN2077206.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15693..16667
                     /locus_tag="U4B19_001460"
     CDS             15693..16667
                     /locus_tag="U4B19_001460"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEN2077207.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16837..18237
                     /locus_tag="U4B19_001461"
     CDS             16837..18237
                     /locus_tag="U4B19_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEN2077208.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18315..19037)
                     /locus_tag="U4B19_001462"
     CDS             complement(18315..19037)
                     /locus_tag="U4B19_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2077209.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19116..19622)
                     /locus_tag="U4B19_001463"
     CDS             complement(19116..19622)
                     /locus_tag="U4B19_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN2077210.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19745..21544
                     /locus_tag="U4B19_001464"
     CDS             19745..21544
                     /locus_tag="U4B19_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEN2077211.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            22038..23426
                     /gene="ltrA"
                     /locus_tag="U4B19_001465"
     CDS             22038..23426
                     /gene="ltrA"
                     /locus_tag="U4B19_001465"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEN2077212.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT         6661 a         5065 c         4329 g         7426 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataacttttt tgttatgttt
       61 ttgaataaat attttcttct gtcttttgga caagtaataa tataccaact aaatttagct
      121 tggtcaacga attttttgta ttttttcaag tttttttcaa taaacgatta ttcataactt
      181 ttctttcttt atcgttcgtt ttcgtcttct ttaatgtcta gaatagtcgg cattagcttt
      241 tgaactttcg ttattattta ttcctttcta cacagcaaaa gctgcctaag gaattcctta
      301 gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga attgattgtg
      361 catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca gttggcggca
      421 taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg tcatttccta
      481 gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga tcgttcagtt
      541 ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga tctgtgaatc
      601 gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt ccgtatacaa
      661 acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg ataacatgtc
      721 cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct aatgcttctt
      781 ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca tctaccatat
      841 ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg atttttgctg
      901 ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc attacatcat
      961 tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta tgtgtagtat
     1021 caattccttc attacggaaa acgcggccga tttcataaac tttttccatt ccgccaacaa
     1081 ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg tccagtgcat
     1141 tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc aatactggtg
     1201 tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg ataatttggc
     1261 ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag tagcgttgtc
     1321 tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga cgaagtgctt
     1381 ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct gttttcatga
     1441 tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact tcataggcct
     1501 catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga tcttgtagat
     1561 gcgcaaatcc agctttccct tttcctcggc gagtcatcat acgaccagct acactagcgg
     1621 tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca tgcaattctt
     1681 ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct tcacgcaatt
     1741 gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact tgatgttgtt
     1801 gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat aatgccaatg
     1861 aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg aaaaacttag
     1921 aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt gatccagcag
     1981 gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt tcgctgctcg
     2041 aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag ctactttttc
     2101 tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt tttcggctgg
     2161 tgttggttca ggaatcagtt cccctgtttc gagataatgc tgtgtgcggt gaatcatcca
     2221 tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat agtcaagcat
     2281 ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggaa tcgtcaaatg
     2341 ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct gaacccgtgt
     2401 acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg cattttctac
     2461 agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg ggatatttac
     2521 tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa gccattttgc
     2581 tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat ctattatatc
     2641 ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt tatttccgcc
     2701 aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct ctaaagtttt
     2761 tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta agcccgcacc
     2821 aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg gtgcaacaac
     2881 gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt aaatccctcc
     2941 taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt tgttattaca
     3001 gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga tttcttctgc
     3061 acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt tgaattgaac
     3121 aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca atatttcttc
     3181 tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta attgttcttc
     3241 gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga atcctcccgc
     3301 cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg aagggatttg
     3361 ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa caatcggcgt
     3421 ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca ttccttgtgt
     3481 tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac tgagatgcgg
     3541 atttttgatg taacctttca catctccctt tccatttgca tctactacaa ttcctgttgc
     3601 tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg tcgcaccaag
     3661 tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc aagtatcatg
     3721 acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc ggacgaatcc
     3781 gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc tttttccgtt
     3841 tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg gaatgcttct
     3901 tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac aacttaaata
     3961 aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc agatcaattg
     4021 acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt gttgagaatc
     4081 tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt caaaatcacg
     4141 tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg cttcttcaaa
     4201 agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag gcatcacacc
     4261 gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca aaagtttttc
     4321 agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt tatcatgagc
     4381 ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt ccgagtaagc
     4441 ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat attgaacagg
     4501 acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag ctgtcgcttg
     4561 ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt cagctgtacg
     4621 tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca agaactgatc
     4681 ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga tcgttacttt
     4741 gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag cttcgtgata
     4801 agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg gacctgcaat
     4861 cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat ttcttctagc
     4921 agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa aacctggtgt
     4981 ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc gtgcatggac
     5041 tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct ggcggtcaaa
     5101 acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg cagcaatgac
     5161 gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt tcaatgtttg
     5221 ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta ccgcatcaat
     5281 ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca aatcacgaac
     5341 acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga tcgagtaaaa
     5401 cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg ttcctggagg
     5461 tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc ggcgaggatc
     5521 tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac ccgctacatc
     5581 agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt taccaaagtt
     5641 catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca tgtaaaagaa
     5701 aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata taccgctgct
     5761 tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg ttacttggct
     5821 caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg tatctgttga
     5881 acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt attcaccagt
     5941 gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc ctgcttgtag
     6001 ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat taccaaaaat
     6061 gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg catttttcat
     6121 catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta atttaacagt
     6181 tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca gcattaattt
     6241 gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata tgcttctgca
     6301 taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac ataatctgct
     6361 tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt gactgatttt
     6421 gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct gtcaatgata
     6481 tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt gacgattttt
     6541 acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa atccaattcc
     6601 aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc ttttaagaca
     6661 cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc tagttctttg
     6721 caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt ttccaacatc
     6781 aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag tgtattttat
     6841 cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta tatatatttc
     6901 ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact ttctgatcga
     6961 taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg tctcctgctt
     7021 ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga gaaaatagag
     7081 accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga ccaatccatt
     7141 ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg tcttctgtct
     7201 ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct cttttcagta
     7261 tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac tggtctaaaa
     7321 tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct ttgtcgaaaa
     7381 aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt tctgcagaaa
     7441 atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc atatattttt
     7501 gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt ctcaacgcct
     7561 gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt ctctggatat
     7621 ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct gcataagtat
     7681 ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta gcaaatggtt
     7741 gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt ttcattaaca
     7801 ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat tgtccttctt
     7861 ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct atggcggatt
     7921 tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca caatatgagc
     7981 gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg tggacgatac
     8041 ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa accatcgaat
     8101 caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat ccttgttcat
     8161 tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct ttttcaacgt
     8221 tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt ctaaaacgac
     8281 aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat tatttgcggc
     8341 tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc gcttcaatga
     8401 agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga agtcttcttt
     8461 ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat ttcggttgtc
     8521 acggggatct ttttgttgat attttttagg acggctttct tctccagatt cagcaggttg
     8581 ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg aaataacttt
     8641 aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat agccatttga
     8701 cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa acgcaccaaa
     8761 atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg acataaaaaa
     8821 aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag ttgtagaact
     8881 agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata taggataaac
     8941 tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta catactcatc
     9001 atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga cttctttcat
     9061 ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca tgtcatggaa
     9121 aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg cagccagtct
     9181 gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact ttttctttgc
     9241 atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga ttcgctccaa
     9301 tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa tctatttctc
     9361 tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat cttctttttt
     9421 ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa actggacttt
     9481 caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt tgatttgaat
     9541 tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc gggaaatttt
     9601 taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta tttaatcgca
     9661 ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga cgaagacgaa
     9721 tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg acaaaaaaag
     9781 cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa tacagcagca
     9841 gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc cgttctttcc
     9901 aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc acacctaatc
     9961 ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga aaagaagtag
    10021 tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga aacgagttct
    10081 tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg gaaaaaacaa
    10141 ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg atataaggga
    10201 gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat ctaagataga
    10261 ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga gtcaacgcag
    10321 gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact aaaggctgac
    10381 ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg taaacttcca
    10441 aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat ccactataaa
    10501 tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac ggctgtttga
    10561 acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca tagtagtgaa
    10621 ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc gtccctgttt
    10681 ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc acaaaaacac
    10741 gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat cttccttgga
    10801 aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa actacttgga
    10861 tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta aatgcaaaga
    10921 caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt tgcaaagctt
    10981 gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac tgcggaagac
    11041 cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga taaacataaa
    11101 aaccttcgtc gccgactaag ttttggaggg gaattcggta aagggcactt aatacttttg
    11161 ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc atttctttat
    11221 gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca acgctttgac
    11281 aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga tcgtcaagcg
    11341 tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg ctgtcagtgc
    11401 ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag taaagactat
    11461 atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca ttttgatctg
    11521 cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt cgagcagatc
    11581 atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt tatagatctc
    11641 gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga caccgaggtc
    11701 gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag aaacagcttc
    11761 gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat gctgctgagc
    11821 acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct tgaatccaga
    11881 tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt cgttaaggat
    11941 cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc ctacccgccc
    12001 tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga acaacgtatt
    12061 tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa catcatattg
    12121 accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt gcccatgagc
    12181 ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt gttcgatcgt
    12241 ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc gatggattgc
    12301 ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat aacgattcgc
    12361 aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat gcaaagttct
    12421 cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact gtttcaatcg
    12481 ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac ccaaatcgct
    12541 aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa cttgacctgt
    12601 acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac ttaatagccc
    12661 gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct gctgtgccaa
    12721 gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt taaaggccgc
    12781 tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc gatccattgg
    12841 tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt catcagtttc
    12901 agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc caaaagcata
    12961 tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga tatcctcgat
    13021 ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct tattgatgcg
    13081 aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag taacaggaat
    13141 aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa cgccatctac
    13201 ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga caacgtaatc
    13261 tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg tttgacgtct
    13321 cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta ctattttttc
    13381 ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct gtcctctgat
    13441 caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca tttgttctgc
    13501 tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt gcttgcgcca
    13561 acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca tcgtccgata
    13621 ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga agagtgagaa
    13681 aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg aaaatgtctg
    13741 ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg cttccctttc
    13801 gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta aactatttga
    13861 agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt agaattttgc
    13921 ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt aatcttgcaa
    13981 aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca ttttttgcat
    14041 ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg ttggggaaac
    14101 ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat accgcataga
    14161 atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta acgggtaaat
    14221 atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct cttctcgtac
    14281 atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat caccaatatg
    14341 ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt gcaaagatgc
    14401 gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg ttgcaactct
    14461 ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt catccactgg
    14521 aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta actgattcgc
    14581 ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa acgctcgagc
    14641 aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag gcattttttc
    14701 ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt gaataatatc
    14761 cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac gaatgccctt
    14821 ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc aaaggctgat
    14881 tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct tctggcctgc
    14941 cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata atacttttga
    15001 taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc actgccagat
    15061 ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa gtcattagtg
    15121 ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa aggatctttt
    15181 ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg aaagtagctt
    15241 gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt gtatattggt
    15301 atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc cacctaattc
    15361 taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg agcttttttc
    15421 ttttttacac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc taaaagagaa
    15481 tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa taattgatat
    15541 ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc ttgttatctg
    15601 ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca taagcatggg
    15661 aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa aaaaaagatc
    15721 atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc tttgctctag
    15781 ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct aaaactgaag
    15841 gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa atctattctg
    15901 ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc gcaccacaaa
    15961 aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac cgcgatgttg
    16021 tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct aacccagtag
    16081 atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc gtaatcggtt
    16141 caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta gttgacgttg
    16201 atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa ttcccagttt
    16261 ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac aacccagaaa
    16321 tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct tacacaatta
    16381 tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt atcactcgtg
    16441 ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat ggcgaatatg
    16501 gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt atccaaaaag
    16561 taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct gctaagcaat
    16621 taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct ttgaacatga
    16681 cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat ggtttctaaa
    16741 ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta tcatgagacc
    16801 gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc gcatcgaaaa
    16861 aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct gatcagcttt
    16921 tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga aataaatgga
    16981 ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga agcttattct
    17041 gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc aaaatccgag
    17101 ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga acaaaacgga
    17161 tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga tcctgtcagt
    17221 caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct gactgatttg
    17281 aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga ctctttcgtt
    17341 atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa acaattaaaa
    17401 agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga aaaaccaaaa
    17461 gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc tatcgcaaat
    17521 gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag tgatattatg
    17581 agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt acgtcaatat
    17641 gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt taaaagtaca
    17701 aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca aacagacagt
    17761 gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg ttcacccatt
    17821 gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat cgaagttgat
    17881 ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga aacagatatc
    17941 gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg gaataaagaa
    18001 gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag tccggtaaac
    18061 tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg gcaacctgaa
    18121 tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac cccaccaagt
    18181 gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt cttctaacaa
    18241 aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct ttttattatc
    18301 taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc atcatcgtct
    18361 tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa atcaatcgtc
    18421 gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc caactcatct
    18481 cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg agaagtttgc
    18541 agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc aacagtcaac
    18601 ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac tgaacccgtt
    18661 tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc tgtccgatta
    18721 cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata tccttcatac
    18781 gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat cgcccgttcc
    18841 acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg caaagctgga
    18901 ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg agaaagtttc
    18961 tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat attatgccat
    19021 ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac ttctattcta
    19081 gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt gaatgccgca
    19141 aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat gacatcttgg
    19201 aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc tgcgtaccct
    19261 gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt ggggaaaaca
    19321 cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc cttccttaaa
    19381 aagaactcta aaaatttggc gtagcccaga tggttgatac tcaccatact tccaccataa
    19441 ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca gctttctaat
    19501 cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa gattataacg
    19561 actatcagtg cgattactaa ataaatattt ccgtttttca tttgtttttt tgaagaattc
    19621 ataagtcacc tctgtatttt atcttagact ttttttgaag aaacaacaat cattttcata
    19681 gagacttctt ccataaaaaa gtgctatact taattttgta ttacaatatg aagggagttt
    19741 tttcatgagt tattcattaa attggaatct agattctatt ttttccggag gcagtcattc
    19801 tgatgctttg aaccaacgga tgaagcaatt ggaagaccaa atgaacgagt attatcaccg
    19861 cgtaacaaaa tggtcaccta gttctgacaa tgcagagcag ttaaacgcta ttcttcaatt
    19921 gcaagaaacg attacgaatg gttttaccca atgcagcagc tatattactg cattgctttc
    19981 tgccaatgtc aatgattcag atgcaaaggt cttatcaggc aagctttacg caatgctgcc
    20041 tcgtctgcaa tcagccgaaa ctgtattgtc aaagaaattt gcagaaatat ctgacaatga
    20101 ttggaatcag atgctttctc aatcttcgtt tgaaacaatt gcttttcgtt taaacgagat
    20161 tcgtcgtgat ggctcacaat tattatcaga agctgaagaa aacattataa atacgctttc
    20221 tttagatggt ctaaatgctt ggagcagtca ttatgatacg attgtagcca gcatttcgat
    20281 tcccttcgaa caagatggac aagtagtcga attatctgct ggacaagcct tcaataagat
    20341 gatgggcgat ccagatccaa aagtcagaga aacacttttc gcagcatggg aagaagcctg
    20401 gaaagaaaag gcacccctct tcacggatac tttgaatcat ttagatggtt ttagactttc
    20461 tgactatgaa cttcatggtg tcacagattt tctacaaaaa ccattagagt acaatcggtt
    20521 gaagaaagaa acattgtctg tcatgtggga tacaatccag aaaaacaaac aaccccttgt
    20581 taattacttg actagaaaag ctaatctttt cggtaaagaa aaaatggaat ggcaagatca
    20641 agatgcacct attattttag gcgatttgaa agaaaaaacc tttagttttg acgaagcagc
    20701 agcatttatt atcgaaaact ttaacaaatt cagtccaaaa atggctaaat ttgcccaatc
    20761 tgcttttgaa aagagctgga tcgaagcaga agatcgtcct gggaaacgtc ctggtggtta
    20821 ttgtactgag ttaccagaaa caaaagaatc aaggatcttt atgacctaca gtaattctgt
    20881 taatgaggtt gcgacacttg ctcatgaact aggacatgct ttccatagca gcacgatgtg
    20941 ggatttacct tctttaaatc gcgaatatgc catgaatgtc gcagaaacag caagtacatt
    21001 cgcagaattg atcgtagccg atgctacatt aaaagtcgca aaaacaaaag aagaaaaaat
    21061 caatttgcta gatacaaaac tgcaaaatgc tttagctatg ttcatgaata tccattcccg
    21121 attcattttt gaaaatcgat tctatgaagc acggcaagaa ggattagtct cagaagataa
    21181 gatcacagag atgatggttg aggcgcaaaa agaaggctat caaggagctt tagctactta
    21241 ccatccatat ttctgggctg caaaattgca tttctttatt gatgacgtac cgttttataa
    21301 cttcccatac acatttggct acctctttag tttgggcatc tatgcttacg ccaaccaaaa
    21361 gggagcaaat tttgaagaag aatatatcca tttactacgc gatactgcct caatgacgac
    21421 ggaagatctt gcgaagaaac atttgaatgt cgatcttacc caaccagatt tttggcaagc
    21481 aggtatcgac atggtgactg aagatatcca aacgttcatg gaattgacag aagaatttat
    21541 ctgatttcta ttttatcaca ataaaaaaag agttagtcca gaatgggcta actctttttg
    21601 atgtgcgccc tgcatgggtg aaaacttggt ggtgaaagtc cactacaggc ttggcagtag
    21661 gaactgttag cgaaagacaa ggcggcaatc gtgaggttgt ggctgaagga agtcggacgc
    21721 aaactcctga actgacgaac agaaactaga tacaaggctg aattaggtcg gataagtctt
    21781 catcacaaga caaagtccaa tactgcccga aacctataca gtaaatctag cagttacatg
    21841 ggaggaaagt tttcactctt accgggggag atctcatgag ggtgttgtgg gcgttgaatg
    21901 aaaacaaacc agcacaacga tcatggcgac atgatggtga atcatgagaa gtcagccgag
    21961 gtcatagtag taacgacact gaccgttacg aaggactgaa caataagaat cttgaataat
    22021 ttaggaggtg tgagaagatg cgacgaccgc agaaaacaag aaaacttggc tatcatcaaa
    22081 gagctagtgt ggaacacgaa gggtatgatg aagtgcgcag cacttcttgc ggtgaacaga
    22141 ggaaacaaga tggtgtagaa aatctgtttg ggagaattct agatcggaat aatctcaatc
    22201 aagcgtatct tcaagtagtc agaaataaag gggcggctgg tgcagatggg atgaccgtcg
    22261 atcaactgct tccttacttg aaagagcacc gcgaggaatt actatccaat ttacgccaag
    22321 ggaactataa acctcagcct gttttgcgag tagaaatacc gaaaccaaac ggcggtgtaa
    22381 gaaaactagg aattcccacg gttattgatc gtatgcttca acaggcaatc aatcaagtgt
    22441 tacaaccgct atttgaacca gaattctcag ataatagtta tgggtttcgc ccaaatcgca
    22501 gcgcacatga cgcagtgaag aaagcaaaat cttattatga acaaggttac cgatatgtgg
    22561 tggacattga tatgaaagcc tactttgaca ccgtaaatca cgataagctg atgtatttca
    22621 ttgagcggaa agtaaaggac aaacaagtcc taaaactgat ccgtcaatac ttaatgagtg
    22681 gtgtaatgga aaatggactc atcacaacaa ctgaagaagg aacgccccaa ggaggcaatc
    22741 tctctcccct gttaagtaac atctacctga acgaatttga tcaagtgcta gaaaatctag
    22801 gacataaatt tgttcgatac gccgatgact gcaatatcta tgttagaagc aaacgagcag
    22861 gacagcgtgt gatgaagaaa tccattcaat ttctcgaaga acaactaaag ttgacggtaa
    22921 atcgagaaaa gagtgcggta ggaagtccat tgaaacgaaa gtttcttgga ttctgcattc
    22981 tgccaacaaa gaaaggtatt aaaattcgcc ctcacgcaaa agcaaaacag cgtgtgaaaa
    23041 gcaagctcaa acaactcact aaaagaaacc gtggaagaag tatccaactt attttaaaag
    23101 aaatcaagca actaatgact ggttggatca actactatgg aattggtgag atgaaagaat
    23161 tcatgaggga gttaaacggt tggttgaagc gaagaatcag acaatatatt tggaaacaat
    23221 ggaagaatcc aagaaccaaa agaaaaaatc tgattcgttt agggatcgat aaagcgaaag
    23281 cgtatgaatg gtcaaataca agaaaaggta tttggtcagt ctccaaaagt catattcttc
    23341 atcgatcatt aacagacaaa gaactggccc gccaaggata tgaggacatc tccttgaagt
    23401 accagttcgt acactcaact tattgaaccg ccgtatacgg gtccgtacgt acggtggtgt
    23461 gagaggggcg gaaggtcagt t
//
LOCUS       DASJTC010000042        23314 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.042, whole genome shotgun sequence.
ACCESSION   DASJTC010000042 DASJTC010000000
VERSION     DASJTC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.042"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(201..908)
                     /gene="deoD"
                     /locus_tag="U4B19_001466"
     CDS             complement(201..908)
                     /gene="deoD"
                     /locus_tag="U4B19_001466"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN2077213.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(925..1743)
                     /locus_tag="U4B19_001467"
     CDS             complement(925..1743)
                     /locus_tag="U4B19_001467"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEN2077214.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1767..2933)
                     /gene="deoB"
                     /locus_tag="U4B19_001468"
     CDS             complement(1767..2933)
                     /gene="deoB"
                     /locus_tag="U4B19_001468"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEN2077215.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(3032..3988)
                     /locus_tag="U4B19_001469"
     CDS             complement(3032..3988)
                     /locus_tag="U4B19_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2077216.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3988..5121)
                     /locus_tag="U4B19_001470"
     CDS             complement(3988..5121)
                     /locus_tag="U4B19_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2077217.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5114..6676)
                     /locus_tag="U4B19_001471"
     CDS             complement(5114..6676)
                     /locus_tag="U4B19_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077218.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6888..7970)
                     /locus_tag="U4B19_001472"
     CDS             complement(6888..7970)
                     /locus_tag="U4B19_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2077219.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(8036..8434)
                     /locus_tag="U4B19_001473"
     CDS             complement(8036..8434)
                     /locus_tag="U4B19_001473"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEN2077220.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8455..9117)
                     /gene="deoC"
                     /locus_tag="U4B19_001474"
     CDS             complement(8455..9117)
                     /gene="deoC"
                     /locus_tag="U4B19_001474"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEN2077221.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9163..10464)
                     /locus_tag="U4B19_001475"
     CDS             complement(9163..10464)
                     /locus_tag="U4B19_001475"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEN2077222.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10605..11621)
                     /locus_tag="U4B19_001476"
     CDS             complement(10605..11621)
                     /locus_tag="U4B19_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2077223.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11929..12534)
                     /locus_tag="U4B19_001477"
     CDS             complement(11929..12534)
                     /locus_tag="U4B19_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2077224.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12730..13653
                     /locus_tag="U4B19_001478"
     CDS             12730..13653
                     /locus_tag="U4B19_001478"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEN2077225.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13712..15277
                     /gene="guaA"
                     /locus_tag="U4B19_001479"
     CDS             13712..15277
                     /gene="guaA"
                     /locus_tag="U4B19_001479"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN2077226.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15768..17399)
                     /locus_tag="U4B19_001480"
     CDS             complement(15768..17399)
                     /locus_tag="U4B19_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEN2077227.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(17413..19086)
                     /locus_tag="U4B19_001481"
     CDS             complement(17413..19086)
                     /locus_tag="U4B19_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEN2077228.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(19099..19416)
                     /locus_tag="U4B19_001482"
     CDS             complement(19099..19416)
                     /locus_tag="U4B19_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2077229.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(19441..20841)
                     /gene="lacG"
                     /locus_tag="U4B19_001483"
     CDS             complement(19441..20841)
                     /gene="lacG"
                     /locus_tag="U4B19_001483"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEN2077230.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            21078..21863
                     /locus_tag="U4B19_001484"
     CDS             21078..21863
                     /locus_tag="U4B19_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2077231.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            21866..22558
                     /locus_tag="U4B19_001485"
     CDS             21866..22558
                     /locus_tag="U4B19_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2077232.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(22832..23203)
                     /locus_tag="U4B19_001486"
     CDS             complement(22832..23203)
                     /locus_tag="U4B19_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEN2077233.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
BASE COUNT         6703 a         5217 c         4212 g         7182 t
ORIGIN      
        1 tattcatcaa acatgaaaag ctgttttttg aaattgttcc ttgtgactca gagttaaacg
       61 gcagattcac aacctcttgg taaacggtgt tcaaaagctt acatttgcaa taataagtcc
      121 agccaagtca aaaacatgag gagctatttt tgcttggatg ttcttattac ttaatgcagg
      181 gcagcttttt catttccttc ttattctgct atcgctgttt ccaatcctac ttcgatcatt
      241 tcattgaacg tagattgacg ttctgcagct gttgtttctt caccggtgat caaatggtcg
      301 ctaacggtca tcaaacttaa tgtctgcaca tggtatttcg cgcctaagta ataaagagcc
      361 gctgcttcca tttccactcc taaaacgcca agttcagcta attggaacgt ctctgtcaaa
      421 tcatctttgt agaaagaatc ttccgaaaga acatttccta catgaaccgt atagcccttt
      481 tctttagcta tctcatatgc tttcagtaat agattgaaat cactgattgg aggaaaatga
      541 aaggcttgga aatttttttc gatcatcgaa gaactggtcg ctgcaccttg tgctagtact
      601 aaatcacgaa catggacgtc ttttgacaat gcaccgcatg tacctactcg aatcaatttc
      661 tttacaccat acgactggat caattcatgg gcatagatgg tggctgatgg cattcccatt
      721 cctgttcctt gaacagaaat acgttctcct ttatatttgc cggtaaagcc taacataccg
      781 cgtacctgat tgtaacaaac tggatcttcc aaaaaagtct ccgcaatata tttagctcgc
      841 aaaggatctc ccggaagtaa aatcttatcg gcgatctcgc cttctttcgc ttcaatatgc
      901 acactcattt tattgttcct cctattacaa taatgtcaat gtttctttaa ccaatgcttt
      961 aaaagattgt ttgactctct gagttgtttc tacaacttct tcatggctca gattcgcttg
     1021 catacctgct gctaagttgg taatacaaga gattcctaac acttttaatc cgctgtgagc
     1081 tgctacgatg acttctggaa cagtcgacat ccctactgca tcggctccta ttgtccgaga
     1141 cattcggatc tccgcaggcg tttcataagt tggtccagaa tagcccatat atacgccttc
     1201 tttaagatcc aaacctagtt tttctgctgc tgcttttgct gttgcttgat agtcaggcgt
     1261 ataagcttga ctcatatctg gaaatctagg gcccattgca tcttcattcg gaccgatcaa
     1321 aggattttca ccagtaaaat tgatatgatc ggtaatcagc atcaaatctc ctggtacaaa
     1381 tgattcgttg acaccaccac atgcattagt cacaatgact gaatgggccc ctagtccggc
     1441 catcacacgt actggatagg tcactgtttg catggaatga ccttcatagt agtggaatct
     1501 cccttgcatg gctaacactt tcttgcctgc cagagaccca taaactagct ttcctgcatg
     1561 cccaactact gtcgaaacag gaaagaacgg gatctcatca taagaaatca ttacagcatc
     1621 ttcgatttca tctgctaatt cacctaatcc tgaacctaga atcaaaccaa aatcaatttc
     1681 ttttactcct ttatcaagga taaactgggt cgtttcttgt aacatctcgc ttaacttggt
     1741 catttgcttt ctcctattta aaaagattat tttaataatt tcaagaagct ttcgccattt
     1801 tcagtcgaag aaacactaaa gttctcagca atcgttgctg aaatatctga ataatatcct
     1861 tgaggcaatg ccccttgttc tttcattttt ttactgtaaa ccaataacgg tacatattct
     1921 cgcgtatgat cggtaccagt aaacgtcgga tcgtttccgt gatcggctgt aatcattagc
     1981 aagtcgtctt cttccatcgc ttcaagcaat tctggaattc gtaagtcaaa ttcttcaatc
     2041 gcatgcgcat agccgactac atcccgacga tgaccgtata atgcatcaaa atctactaaa
     2101 ttggtaaagc tgattcctga aaaatcgttt ttcataactt tcagcagctg gtcaacgcca
     2161 tccatattgc ttttcgtgcg gacagattca gtgattcctt gtccgttgaa aatatcgttg
     2221 atctttccaa cagcaatcac gtcctttcct gcttctttca aagaatcaag gactgtgtgt
     2281 ccgaatggat tcaacgcata atcatggcga ttgcttgttc gtgtgaagtc tcctggttca
     2341 cccacgtacg gacgtgcaat gattcggcca atcatatacg gatcatcttt tgttatatca
     2401 cgaacgaatt ggcaaatccg atataattct tctaaaggaa tgatttcttc atgtgccgcg
     2461 atctgaagga caggatcggc agaagtatat acaattaaat cgcctgtttt catttggtgt
     2521 tcaccgtaat cgtcaataac agctgttccg ctataaggtt tgttacatac gactttacgg
     2581 ccagaaaatt cttcgatttg tttgatcaga tcatcaggga atccattagg aaacacacga
     2641 aatggagttt tgatattcaa tcccatgatt tcccagtggc ctgtcatcgt atctttccct
     2701 acagaaactt cttctaattt cgtcgcatag ccttgatgat ctttcactga ttccacacct
     2761 tcaagaggac ggatcgttcc taaaccaagc tgctctaaat gtggaacgtt caatcctgct
     2821 tctttcgcaa tgtgtcctaa tgtatcagaa ccaacatcac cgaacttatc ggcatctggt
     2881 gcttctccaa ttcctactga atccatgacg atcagatgta cgcgtttaaa catgattatt
     2941 ccttctttct ttttattttt attgccttta aaacaaaaag ggcgagacga aaaacacttc
     3001 ccgtaaccca ccctttttat tcgctaaaag attattttga tttgatatag ttcttcccgt
     3061 ttgcttttgg accagaagct ttccctaaga aaacaaccag cacaatgatt gtcagcaaat
     3121 atggagcaat ttgcagcaat actggcggaa tcgaagaaat aactggtaat tgtgcgccaa
     3181 tgatactcaa gctttgagca aaaccaaaga acaatgaggc acccattgcc cctaaaggat
     3241 tccacttacc aaagatcata gcagccatgg agatgaaccc ttgtccaacg atcgtaccag
     3301 ctgaaaagtt accggaaatc gactgagcaa aaacagcacc gcccataccg cctaacaagc
     3361 cagaaatcag tacgcctgaa taacgcatag catatacatt gatacctaat gtgtcggcag
     3421 cttgaggatt ttcaccaact gaacggaggc gcaagccgaa tttcgtcttg aagatgatca
     3481 cccaagctag gacagcaatc aaaatagcac aataagcagg taagaatgta tttttaaaga
     3541 aaattggtcc aatgaccgga atatctgcta atcctgggaa agaaaaatac cccagattct
     3601 gcgtgatgtt atcagtttga cctttaccat accatgcttt gatcaagaaa acacctaaag
     3661 caggtgccat cagattaatt actgtcccgc tgataatatg gtcagcacgc aaactgactg
     3721 ttgctactgc gtgaatcaga gaaaatagga tacctacgac gccacccact aaaatagcga
     3781 tccatggtgt catagaacca agtgatggac tcatcgtcaa gttgaaaacg atggaactaa
     3841 aagcacccat gaccatgatt ccctctagcc ctacgtttac gatcccgctt cgttcagaaa
     3901 aagttccgcc tagtgacgtt agaattagcg gtgttgaata aaccagtgta gaagtaagga
     3961 tcaaggcaat taattcgata gtcgacatta tagttcgccc ccttcatggt tttgattcgt
     4021 tggtttcgat tctattgtcg caacgatctc ttcttgtttt ttcccttgga agactttagc
     4081 catgacgaaa cgaatcagga agttgatccc gacaaagaag ataatcaaag caatcgatac
     4141 attgacgatc tcaaacggaa cacctgctcc tgtctgcatg ccaagtccac cgatcttcaa
     4201 aatactgaac aagatcgctg ataaaagaat accgattgct gatccagaac caagcaagga
     4261 aacagccatt ccgtcaaatc caatagatag cgaagtcgtt tgaacaaaga agttttgata
     4321 cgttcctaat ccttcaacca caccgccaag ccctgccaaa gcgccagaaa tgaccatcga
     4381 caaaacgatc gtacgtttac tactcattcc tgcatactca gaagcaaaag gattcaatcc
     4441 aacagaacgg atttcaaatc ctaaagtcgt tttcttcatc agcagccaaa cgaaaataag
     4501 cgcgatgatt gctaaaaata atcctgagtt tacgcgagag ccgccaaaag cttgtgttaa
     4561 ccatggtaac cgcagtgagg cattttgact gatcatattg gaagagtcca aacttgagcg
     4621 atagctttca ggcatcactt catgtaaagc aaatgtgctt acgtataata agatatagtt
     4681 catcatgatt gttacgatca cttcacttgt tccaaaaaat gctcgaagta aacctggaac
     4741 agccgctgcc atcgcaccag ctaaggcacc taagatcacg cataacggaa gcaaaacaac
     4801 ttttggcaga tctggaaacg caagtgccac ccagacactg acgatccacc cgcaaagtgc
     4861 ttgtccagaa agcccaatat tgaaaaaccc agctgagtta gctacagaaa aacctaatgc
     4921 tgtaaaaatc aacggcgttg cttgccttaa cgtttcacct atccctcttt gcgaaccaag
     4981 ggctgcatct agcatagaag aataaccttg gatgggctga aaaccaaaag cagccatcaa
     5041 tatcgcgcct aagatcaatc ctaaaacaac tgaaagtaca ggcactaata aattacggaa
     5101 tttttcgctg cgatcattca tgtgcgccag cccccttttc taactctttt cgtgcttctt
     5161 ctaaggtata gcccgccatc aatagaccta attcattttc agatgtttct ttcggatcaa
     5221 cgataccgac aatttctcct tcatggataa cagcaatacg atccgaaaca tttaaaattt
     5281 cttctaattc gaaactgatc agtaacactg ctttgtattt gtccctttgt tcgatcagac
     5341 gtttatgaat aaattcgatc gcacctacat ccagtccacg agttggattt gcaacaatca
     5401 acagatcagg atctcggtcg acttcacgag cgataattgc tttttgctga tttccgcctg
     5461 ataaagcttt tgcgggaaca agttcattcg ttgttcgaac atcatattct tcgatcaatt
     5521 ctctggcatg ttcattcatt actgaataat tcagcatgcc gtttttactg taaggttttt
     5581 gatgataggt ttgtagtgcg atattttcag ataatgtcat atctaggact aacccgtatt
     5641 tatgacggtc ttctggcacg tgtcctacac catgttcagt gatttttcgg ggttttttat
     5701 tagtgatgtc ttcccctttt agcttgatat gtccgctttc tgcctttcgc aaaccagtca
     5761 aagcttggat caactccgac tgcccgtttc catcgattcc agcgatacca agtacttcgc
     5821 cagcacgaac ctctaagttc aagtttttca cggcttctaa tccacgattt tctttcacca
     5881 ctagattttc aatagacaaa acgacttctt gaggcttcgc ttcttttttc atcgttttga
     5941 atgaaaccgt tcttccaacc atcatatcgg ctaattgttg tgaggttacg tctttaacgt
     6001 tgactgtacc gattcctttt ccacggcgga taactgtaca gcggtcagct actgctttga
     6061 tttcatctaa cttatgcgta atcaaaatga tcgacttgcc ttctttgact aattccttca
     6121 tgatcacgat taattcatca atttcttgag gggtcaatac agctgtcggc tcatcaaaaa
     6181 tcagtacatc tgctcctcgg taaagtgttt ttaaaatttc tacccgttgt tccattccaa
     6241 ctgaaatatc acgaacgtaa gcatctgggt tgactgacaa tccatactgt tcagaaactt
     6301 tttggatctc ttttcgtgct tttttatggt caagcatccc tgcgcgacta ggctcgcttc
     6361 ccaacacgat gttttctgtt acagtaaagg catcaacaag cataaagtgc tgatggacca
     6421 tcccaatccc taattggttg gctttggtcg gacttgtgat ctttacttct ttcccacgca
     6481 tcaaaatttt ccctgatgtc ggctctaata atccggaaag cacgttcatc aatgtagatt
     6541 tgccagcacc gttttctcca agcaacgcat gaatttctcc tgcctttact tgtaaattga
     6601 tgttgtcatt tgccttgaat gttccaaatt ctttggtaat attctgcatt tctatgacaa
     6661 tattttcttg aaccaccttt ttttcacatc ctaacgattt ctatctaggg caacgattga
     6721 ctaagattca cgcttagtga gcgttcttct tttgaaacaa acattacttc agggagttgt
     6781 tcccttatcc aaactgtcac aaggcagctt ggataaatga acaattcttg ttctaatcaa
     6841 accatagcct tgtcaaacat gtgtctaaca aggctataga aatggtttta gttttcttca
     6901 ggagtttctg gaacttttac gtcacctgaa atgatttttt cttttgcttc atcaactgct
     6961 tttttcgctt cgtcagaaag ttgtccttct gttagaccaa caccgtcttc tttcaatcca
     7021 taaacagtgt gttcgccgcc agggaattta ccgtccgctg atttttgtgc caaatcttct
     7081 actactgttc caactgcttt cagtgtagat gtcaatgtga agtttttctt ttcgccgttt
     7141 aatgtgtatt cgccttcgtc tgattggtcg cggtcaacac cgataaccca aacttttttg
     7201 tcgcctgatt cgttcaatga ttttgcttct tggaatacac cgttacctgt accgcctgaa
     7261 gcatggaaga tgatatctgc gttttgtgca tacatacctt gagcaattga acgtccttta
     7321 tctggagcag agaagtcacc agcatattga ttcaatactt tgatttcttt tcctaattct
     7381 ttcgcacctg catcaacacc ggctttgaat ccagcatcaa aacgatcgat tacttcacct
     7441 tttacaccgc cgataaagcc gactacgttt gtttcagtcg tataagctgc tgcaacacct
     7501 gcaagataag aagcttcgtt gtctttgaaa gttgcagata caacgttatc taatccatcg
     7561 atgacatcat cgataataac aaagttcgta cctgtattgt ttgttgcttg ttcttggatc
     7621 gctggtttta gtttataacc aataccaaaa atcgttttga atccagcatt caatgcttgg
     7681 tcaatatttg ggatgtaatc tgattcattt gaagattgga aatattggaa gccatcattt
     7741 cctcgtgaaa gtccttgatc tttcccccat ttttctagtc cttcccaagc tgactggttg
     7801 aatgaacggt cgtctacacc acctgtgtct gtgatcaatg ctgcagttgt tgtgtctgat
     7861 gagcttttgc caccggatga atcagtagag ccgttgttgc cgcatgcacc taaaaataac
     7921 cctgctgcta gtgcgacagc acctaaacca aataattttg cttttttcat ttctgtaaag
     7981 cctcctaaaa taattgtttt atttttcaac agtcagctga ctgaatgaac atgagttata
     8041 gatctttttc agtaaatgca taaggcagca gtgcacgaac ggtcgttgcc ttcgtcacac
     8101 cattgtctcc tactagcgta accggcatat caggggaaca gaattctgcc attacttgtc
     8161 gacatgcacc acaaggagag ataggatcgg gggtgtgccc cgctacgact agatgcgtaa
     8221 atgttttttc tccttcagat actgctttga aaaaagctgt gcgctcggca caattcgtta
     8281 atccaaatga tgcattctca atgttgatgc cttgataaat cttgcctgat tccgttacga
     8341 gacaagcgcc taccggaaaa tgggaataag gaacataggc tttatctaac gcttcaatag
     8401 ctgtgttgat ccattcttgt ttgagttctg ccatgagctt ctcctttgaa aaaattaata
     8461 gccttcacca gttgaaccag atacgattgc tactcctgcg cttgttccga ttcttgtagc
     8521 accagcttcg atcattgcca ttgcttcttc ttcgttatgg ataccgccgg aagctttgac
     8581 acccatttct ggtcctacag tttcacgcat caaacggaca tcctctacct ttgctccgcc
     8641 tgttgagaaa ccagttgatg ttttgacaaa atcagcgcct gcagcttttg ctatttcgca
     8701 agctcggatg atttcttctc tatctaataa cgatgtttca ataataactt tgacaagcgc
     8761 acgatctttt gctgcttcaa caactgcttc gatatctttt tggacttgtt gatcatcctt
     8821 tgatttcaaa gcaccgatat tgatgaccat atcgacttca tctgcaccgt tattgatggc
     8881 atctgttgtt tcaaatgctt ttgtctcggg tgtgtttgct cctagaggaa acccgattac
     8941 tgtacatacg gctactggct caccttgtaa ttgttctttc gctagcgcaa cccatgctgg
     9001 attgatgcag acagaataaa aatggtattt ttttgcttct tcgataattt gcatcacagc
     9061 ttctttagat gcgtctgctt tcaaaatagt atggtcgatc attcggttta gttccatttc
     9121 ttttcccctt ttctttaaaa cggtaaagtc cgcttttttc ctttattctg tgatgatgtc
     9181 atgaatcaac gttggcttca ttgccgattc accaatttca atatttttat ataataattc
     9241 aatgacagaa gtgatttctt cgtcgttact atagatcgtc aatagtgact caccttttgt
     9301 cacagtgtca ccaatttttt tatgcagttt caatccaaca gcgtgatcaa tatcttcttc
     9361 tttggtttta cgcccagcac ctagcatcat tgcagcaatt cctaattcat ttgccacgat
     9421 ttttgacaca actccgcttt gtttagcagg aagctccatt gtgtagcgtg cagtcaaaat
     9481 tcgttctgga tgttccacga tcgctgggtc tcccccttga ttttggatca tttctttgaa
     9541 ttttgctaac gctttacctg attccagagc ttcttgaagc aacgttcttg cttcatcggt
     9601 tgtcttagct ttgccagcaa gaacaaccat ttggcttcct aaggcgtagc acatttctac
     9661 tagatcttct ggaccatttc cttgtaatgt ttcgattgcc tcgacaactt ctagactatt
     9721 tccgatagct tcgccaagag gttctgacat atctgagata accgccactg tttctcggtt
     9781 agccagtttc ccaatgctcg tcatggtctt tgccagacgt cttgcatctt cgatgttctt
     9841 catgaaagca ccgtctccag tggtcacatc caaaacgatt gcatctgctc ctgccgcgat
     9901 ttttttactc ataatcgaac tggcaatcag cggaagagaa tcaacggtcg ctgtgacgtc
     9961 acgcaaggca tacaattttt tgtccgccgg tgccaagttt cctgattgac caattaccgc
    10021 tactttactt tcattaacaa tgcgtacaaa ttcttcatcc gaaagctcga tctgaaatcc
    10081 gggaattgct tctagcttgt ctagtgtacc acctgtgtaa cctagaccgc gtccagacat
    10141 tttagccact gtcactccga cactagctac taggggggct aaaatcaatg tggtcgtgtc
    10201 accaacgcca cctgtcgaat gcttatctac ctttattcct tcaagagaac tcaaatcgat
    10261 gacatcacca gaattagcaa tcgccaatgt taagttcgtg atttcttcat ttgtcatgtc
    10321 ttgataaaaa attgccatca ataatgcact gacttgataa tctggaatct ttccattcgt
    10381 atagttcgtg acgatgtact cgatttcctc tttcgacagc tcgtttccat ccctcttttt
    10441 ttcaatcaga tccaccattc gcattgcctg atcctccttg tcttcattcg gtattataca
    10501 ttatttttag gaaaggtaaa ccactttagt aaactggttt actaaaaata aaaaaagcac
    10561 taactagcct ttgtaaatgc ttacataagc aatataccgc cgagttagcc ctttgtcaat
    10621 acttttacgc tctcacgaat aattaatttt gtttcgaaca ctttgttcgg aacttttctt
    10681 tgaggaaatt cgatcgtatc gatcaggaaa cgtgcagcat aaaatccaat atcaaaactt
    10741 ggctgctgca ctgtcgtcaa ttgcggaatc atgtattctg acagctttaa tccatcgaat
    10801 cctatgactg agatatcttc tgggattttt ttgcccaatt catcgatcgc ctgatagcat
    10861 ccgatcgcca tggcatcatt cccgcaaaaa atagccgtga tatctttatg tttgagtaat
    10921 ttttttgatg cagtatatcc accttggaca gtcaactctc catgttcaac aaatttccca
    10981 ttaaaaggca ggccatgatc tttcagcgca tgacgatatc cgttgaaacg ttcttctaat
    11041 tgatagtatc cagtggtttc cttcagcatg ccgatcttcg tatggccatt ttcaataaac
    11101 agcgaaacag cttgataggc gccctcatat tcctttacga taagccgtcc gttatcccga
    11161 cgattcaatc cgcgatcgat taaaatgatt ggcatttttt gataaaaacc gctgtctaat
    11221 gcatatttct caggtaaaat atttggtgtg gccagcaaga ttccgtccac tgagcggtgg
    11281 gaaagctctt gtaaatactt gatctcattt tcttggctat gacgagaatt gcataacatg
    11341 atcacatagc ccaaaggatt caaatagctc tctacacctt cgatcagttt ggagaagaag
    11401 aaatcagtga catcagggac gatcattccg atggtctttg aatgtcgatt gatcacattt
    11461 gaagcgaaat aatcgggctt ataatgatgt tcattcacga tcgacaacac tttttttctt
    11521 gtcttatcac taaatctgct tcctttgtta ttcaaaatct gtgagacagt tgttactgat
    11581 acgcctgcca tttctgcgat atctctgatg gtcagcttca tcctattggc tcccttctat
    11641 ctaaattccc tatcagtgta ccaaaacccc caagaaaaga atagagaaaa aaggaaagta
    11701 tgttttttga taaaaacctc ctttatctac cgacttggca gccttctata caagttctgt
    11761 atcttattta ttttatcgta aaaaacaacg actctgttcc tactatttct ttttgaagaa
    11821 aaaatagttc tttgttttta aaaacttctc aacaaaaaaa gaactgatac gtacccgtac
    11881 atgcacgccc agttccactc tttcgtcgct ccactatttt tcagctagtt attctttcac
    11941 acttttgatg atataatatc ctttatcttt tgtgacgatt tctgcattac caaatgtttc
    12001 ttccattttc ttttgcgcac tcggcgctcc ttgtttcttc tggatcacaa ttgtcagtgt
    12061 accgcccttc ttcaaccgag gatatgcgcc tgtcaaaatt tcatgtacaa ctttttttcc
    12121 agcacggatc ggcggattgc ttacgatagc tgcataggtt tcttcatgaa ctgcttcata
    12181 gatattcgat tgatggatat caacatgttc gatttggtta cgctttgcgt ttccttgcgc
    12241 tagacttacc gctcgttcat taatgtcgac catttcaaca aaccgctgtg tcgcaaatgc
    12301 caaagctaac ccaatcggtc catacccgca tcccacatct aagatctttc cttcatcagg
    12361 taactcttcc caatcgaaag catcaatcag gacacgtgaa ccataatcca ctgtttcacg
    12421 tgaaaataca ccagaatctg ttacaaattg aaaggttctg ccttttaact caaaagccca
    12481 ttgttcaaaa tcgtgcgccg tatctgggcg ttcagaatag taatgatttg tcatgtcttc
    12541 catccttctc atttctttca tgacaacagt ctatcctttt atgtatccgt gcgcaagtca
    12601 agaataagac tccgtttctt tctattataa agattataca aaaagatcgg caatctctct
    12661 taagtggtgg ggtttgcgtt tattttgtgt taaaatagtt ccattgatgg gagaacgcag
    12721 gaggctaata tgaatgatcc aatgaactac taccgtgtgg caagagaaga atggcgagaa
    12781 ttttatcgta atgggcaagc acctttgaca caagatgaac tagataatat caaaagtttg
    12841 aatgaccgca tttccatgca ggatgttcga gatatctatg tacctcttgc tcatctgatt
    12901 tatctttata tgaaagaatt tgaatctttg ttcttgagta aaggtttatt tcttcataaa
    12961 tacgtagaag taccgccttt tattatcggg atcgccggaa gtgtagccgt tggaaaaagt
    13021 acaacagcca gactgctgca gacgattttg tctcgtacct tcaaacgaag aaacgttcag
    13081 ctgatcacga ctgacggatt tctttatcca aatcgtatat taaaagagcg tggaatcatg
    13141 gaccgtaaag gatttcctga aagttatgat atggagatgc tgatcgattt cttgaaccgt
    13201 gtaaaatctg gtcaggaaga gataaaagta cctgtttact cacatgatgt gtatgatatc
    13261 attccagaca agtacgaatt gatccagcag cctgatatct tgatcgttga ggggatcaat
    13321 acgcttcaat tacctgcgaa tcagcaaatc tacgtcagcg acttttttga tttttctgtc
    13381 tatgtcgatg ccgatcccgt gctgatcgaa aaatggtatt tggatcgttt tggtgcgtta
    13441 ctggatacgg ctttccaaaa tccagagaat tattattatc gttttgctat cggagatcgt
    13501 gaagaagcct ttaaaatggc aaaagatgta tggaaaaatg tgaatttgaa aaatttacag
    13561 gaatatatct tgcctacacg aggaagagcc gatataattc tccataaaac tgaaaatcat
    13621 atcatcgatt cgattttcat gcgaaaatat tagcatttta aaatagtttg actattttga
    13681 gataaaattt aaagggatag gggtaataaa tttgacaaac gttgccgata tgacgaacgt
    13741 tgaaaagatt atcgtattag actacggtag tcagtataac caattgatca caagacgtat
    13801 tcgtgatctt ggagtctttt ccgaattatt gagccaccgc attactgcag aagaagtaaa
    13861 agcgatgaat ccaaagggaa tcattctttc tggcggacca aacagtgtat atgatgagaa
    13921 tgcatttggg atcgatcctg agatcttcaa tttaggtatt cctgtattag ggatttgcta
    13981 tggtatgcaa ttgatcacgt acaaacttgg cggttccgta gagcctgcta aaaaccgcga
    14041 atatgggaaa gccttgttag aagtaacaag cgatcaagct gtcttgttta aagaaacacc
    14101 aaaacaacaa acagtatgga tgagccatgg tgacttggta actaaagttc ctgaaggctt
    14161 tgaaacagtc ggaacaagca aagattgtcc gattgcttct atccaagaca caacacgtaa
    14221 tttttatggt atccaattcc atgcagaagt acgtcattcc gaatacggaa tcgaattgct
    14281 gcgccacttt gcctttgatg tttgtcactg tgaaggtaac tggacaatgg ataactttat
    14341 cgatatgcaa gtagaaaaaa tccgtgaaca agttggtgac aaaaaagtct tgcttggtct
    14401 ttcaggtggc gttgattcta gtgtcgttgg ggttctttta caaaaagcga tcggtgacca
    14461 attgacatct atctttgttg accacggtct gctacgcaaa ggcgaagcgg aacaagtaat
    14521 ggaaagtcta ggcggaaaat tcggcttgaa catcatcaaa gtcaatgcaa gagatcgctt
    14581 cttagacaag ttgaaaggtg tatctgaccc agaacaaaaa cgtaaaatca ttgggaatga
    14641 attcgtatat gtttttgatg acgaagcaac aaaacttgct ggtgaagaag gcgtctcttt
    14701 cctagcacaa ggaacgttgt atactgacgt tatcgaatct ggtacagaaa cagcacagac
    14761 gatcaaatct caccataatg taggtggact gccagaagat atgcaatttg aattgatcga
    14821 accattaaac acattattca aagacgaagt ccgtgaatta gggactcagt taggcatgcc
    14881 tgattcaatc gtttggcgtc agccattccc tggtcctggt cttggtattc gtgttcttgg
    14941 cgaaatcact gaagacaagc tagaaatcgt acgcgaatct gatgctatct tgcgtgaaga
    15001 aatcgcagct gctggacttg accgtgatat ctggcaatat ttcactgttc ttccagggat
    15061 ccgctctgtc ggtgttatgg gtgacggacg tacttacgac tacacagtcg gtatccgtgc
    15121 tgtaacttca atcgacggaa tgaccgctga ctttgcccgt atcccatggg aagtcttgca
    15181 aaaaatctct gtacgtatcg taaacgaagt agcccacgtc aaccgtatcg tgtatgacat
    15241 cacttctaaa ccaccagcaa cagtggagtg ggaataaaga gccaacataa tatggagggg
    15301 gaatccctat aaatcaacat tcttcaatag ctaaaatgct tgattttcat taaaaataag
    15361 atggttccct accaaaagag actataatat aagttaattt gtttcattct tatcataaaa
    15421 agaagttctc gaaaaacgaa ctgttttccg agaacttttt tctatccatc taagtgctca
    15481 aatcatattc acgacggaat tctaaggtta ccctttctta aacaaacttt atgttatctg
    15541 cacaactttt tgcaattttt tcattgcttc ttacgaaatc gttgaactct ctttagcata
    15601 atacaaagtg tagatgaagt ctggtgcttt actcgttttg gtaaaaatga ctggaaatcg
    15661 tttctggtta tttttggcaa aggcgaggtg caggttcgat tcctgccttc tacgctaacg
    15721 atgtggctga agggaacctc cgatcttgaa tcgaaacgat ccgcagtcta ttgtttcacc
    15781 aatgaaacaa aatcaacgtt cgtccaagca tttcctgaag ccttgctata aaccccaatt
    15841 tcacaaacac ctgttgtaat tggaatgtct ttgattgtaa tttttgtcca ttcacttgtt
    15901 ttaacaaccg cagtatttag ttccgctcca ccataatttt tgatatataa ctgattagta
    15961 tcaaactctc ccccagacat tacataggca ctcaatgtgt aagttccttt gcttatgcca
    16021 ctaacatttt gataagctga tgcctcgtat gcttgatcct gccaaaaagt tagtttgtag
    16081 tccccaaaaa aagtgcccgc ttccgttttg atcgtaccct tagcggttcc ttgttgaaac
    16141 caggtttgcc aattggaagg actggtcgtt ggtccatcgc tttcgaaact tgcattttta
    16201 actagattta tttgcgcccc tgcttgttta aacgcacgca agcttggcaa ggcgtttcca
    16261 gcaaaatcaa acatcgcttg gttgtcccat tcgtttccca tagtggattg aacactgaga
    16321 taattcatac ctgcttgtgt tgcccaactc acatttccgt tgtaccaaag aggttcccaa
    16381 tagaagaagc ccgccccgcg attattcggt actgcctgaa tccgatcggc aagatccagc
    16441 aaatactctg cttgtcctgt cggtgttggt tcatatcccc ccgcagctgc ttcggcttca
    16501 ccaaaagcat ttggcatagt atcggcattc gctgttgtat gcccataagc tgtttcaact
    16561 acgatcactt ctttaccata ccgttggctg atcgcattca tattggtttg aagttcattc
    16621 attgaaccgt gccaatacgg ataatacgac actccaatta aatcaaatga cacacctcga
    16681 ctcgtgattt catcaaacca ccaagtaaat gctcctgtat cgccaccatc agctaagtgg
    16741 agcattactg gagttgtttt aggttgagtg tctcgaacag cttggatacc agacttcaaa
    16801 aaagcagcca atcgatcaaa ttctcccccg ccttctcccc aagacttgcc ataaggccag
    16861 agcattccgc tattcagttc attgccgatc tgaaccatat caggataaac gtccagctgc
    16921 ttcatttccg acagaacgtt ctttgtatag tcgtacaatg ccgtattcat ttcttcaaag
    16981 gtcagacctt gccatgcttt aggaagattc tgtttcccag gatcaaccca aaaatcactg
    17041 tagtgaaaat ccagcagcac cttaagtccc agattttttg ctcgttttgc aaggtcaatc
    17101 gtcgtttcca gatcatttgt ccctgctcca taactttctc cattactatt ataaggatcc
    17161 tgccacaaac gcagtcgaac atagttgaca ccattatttt ttaaaatctc taaggcatct
    17221 tttgaaatat cgtcttcata atattttgca ccagattttt ccatatctga caggatcgaa
    17281 atatctgctc cgcgaataaa ttcgctggca gatgcttcaa cagtttttga aacagccccc
    17341 aaagccccta acacagctgc aaataaaaca gctttacgca accaaattcc cattttcatt
    17401 atggctcctc ctttattgaa attggttcag aataaactga acagcttttt caggatctct
    17461 cgttaatccg atatattctg ctccttttgt cgcaaccagc ttgattccta agcgatcggt
    17521 gtcttctttg atatcatcat agtaagaatt gacttgcggt gctaagacca ccatatcaaa
    17581 atcctgcagc atttcgtaat gagaaccata agcacccgcc attgcactga tcggtgcttt
    17641 cgattcagat gcaccttttt ttaaagcatt agctaacatc ccacttgtcc ctgcgccagc
    17701 acaaagtacc aacacattga tttctttatt tgcatcgctg ataacttgac tcgctgccac
    17761 aggtacttcc tcacgtacat cggcagaagc accttcctcg gttgcgatca cctctgcttt
    17821 ttgtgcttct ttctctacta agacagcatc gtatgctttg caaaatggta gatagatcag
    17881 gaagtcaacg acgattagaa ccgctgctag aacaaaagct aagataccca tgcccgttcc
    17941 tagaaccaaa ccgatcggtg ctggagttgc ccaaggcaaa acgtacataa agctgttcat
    18001 gcccaataca tcgacaaaaa acttgaacag gcaaacgttt acaacaggtg ttaacaaaaa
    18061 tggaataaaa aaataagggt tcaaaatcat gggtgccgca aatagcaatg gttcgttgac
    18121 agcgaaactt actggaataa aggaagcttt cccaactgcc ttcaactgct tagacttagc
    18181 aaagagtagg aagataaaag gaacaaccaa tgttgcccca gttcctccca tagttcctac
    18241 aaaattccct aaacccaccg tcagtacttt agaagcttgt tcaccggaat tgaacaattg
    18301 cagattggtt tccacattgg catagataat tgccgcaatt gccggctcca cgatcgacgg
    18361 tccatgaaca ccgacaaacc aaaacagcgc catcgctccc cagataatag cgattcctaa
    18421 gtacccgtca gctgctgtaa atagcggctg cagcaccgta atgattgctt ctgcaaatga
    18481 gatttggaag atttgtcgaa tgcctaaatc aattaaaacg gccgcaaata ctgaaaaaga
    18541 gaaaggaaaa atatctcgga aggtttgcga aatcgttcca ggaacttctt taggcatttt
    18601 aatggtaata tctttcagca cacaaaactt atataaatta actgttaaaa atgcagcgac
    18661 aaaggaagcc agtagtccct ttgtccccat atattccgtt gaaaatccac cttcaatcgg
    18721 agagacgctt aacaacagga aacttaccac agaggccagc atcacagagg tggtgttgat
    18781 tactttgttt ttcggtaagc gacgattcat cgaatcagcc aagcagcgag cagtggttgc
    18841 tgaaacgagt aaccctacaa cacccatcga ataattatag actttccata gccatgtgga
    18901 gacagattca ggccattggt aaccaaaaat ctcaggaacc gcagcaatca aaatgaaaat
    18961 gcttgaaaat aaaatagctg gcatcgctgt taaaaatccg tcacgaacgg ctcctaaata
    19021 actattacgt gagatttttt caaaaaatgg tttccctttt tcgatttgtt taatgattcc
    19081 atccatagcg ttcttctcct atccttgatt ctgtctgtac agtacaataa agtcttgaat
    19141 cagatctctc agcaataaag tggtcattaa atgatcctgc ccatggataa aaatgaatcc
    19201 catctccatc tctttcccag atgcttcttc cgctaaaatt tttgtttgcg aattatgtgc
    19261 aagggttaaa ttttcgtcgg cttctttcaa cgccgaatca acatgttcaa agtttccttg
    19321 tcttacttct ttcaataatt tcaataatgt actgcgagcg tcgcctgaat atgcaacgat
    19381 ctcaaaacca agcatttgta attcctcttt attcatcttt tcttcctcca ttatcactta
    19441 ttaaataatc gttttggttt cactgaccaa cttgtaccag taagctgatt cttttgggta
    19501 acgcttctgg gtttcaaaat caacataaaa caagccatac cttttgttat acccatttgt
    19561 ccatgagaaa agatccataa gcgaccacaa aaaatacccc ttcacattta ccccggctgt
    19621 tatcgccttg cttaaggatt ccaaatagac tctcaaatag tcgattctcg gctgatccat
    19681 aataatgcca tcttcaaact gatctttata ccccatcccg ttctcagtga tatagatttt
    19741 gttgtaatgt gggtaatcgc ttttaattcg cagcaacaag tcatatagac cttctggata
    19801 aatcagccag tcccaatcag ttcgaggaat tccttcttta taaatacgtt ctccgattcc
    19861 cttcacttta taaacagagg tccctttttc acccgtaccg ttatgatgga ttgcattttc
    19921 tccatcgtaa gctttgacaa aatggcattg gtagtgattg atccctaagt agtcattacg
    19981 cgtggatgct tttttcaatt caacgaaatc ttcttcagga aaatgatagc tggcctgatt
    20041 tgcttcacat atctcatcaa gagcagtcaa ggtttcagtt gaataatagc ctaaataggt
    20101 agcatctaat aagaaacgga tcgacaatgc atcgtctaaa aaggcagcat gcttatcttc
    20161 cggcgcgtct gttgccgcat atttcgtctc taatgagtga actacaccaa tttcacccgg
    20221 aagttcgttt tctttgaaat agttcactac acgggcatga gcaaccatca tattatgaag
    20281 acaggcgacg atctttgtaa aatcatattt gattcctggt gggaaaacac ctaacaaata
    20341 ttgattggtt gcaactggat agatttcatt aaaggtactc cacaccttga cttctttgaa
    20401 ttcatgaaaa caaaaaatgg cataggaaac aaatgcttct attgtttctc gattcaaaaa
    20461 atctccatgg tcaaacaaac gtttaggggt atcaaaatga tgcaacgtca caaacggcgt
    20521 tacgtttctt ttcttacatt cttcgaacac acgatgataa aaagcgattc cttctggatt
    20581 tggtttccca gctccatcag gaaaaatgcg gctccaagca atggataatc ttagaccatt
    20641 tacaccaaaa cgttcacaca attcaatgtc ctttgcatat tgatgataaa aatcacttgc
    20701 cggatcggga ctgaatcgcc cttgttcctc taaaaagtca tcccaagcaa cagcgccttt
    20761 gccaccttcc ttagtggctc cttcaacctg ataggctgct gtggctccgc caaaaataaa
    20821 atcttctggt aatttaagca tgtacatctc tcctcaaatc tataatcagt tttcgctctt
    20881 tctaactgat tgcattgtaa acgcatccaa aacatttgtc aacaaaaaca aacacaaata
    20941 atcataaaaa ccagaaaaca gcaataaaat ctattgtaac aaacataaaa accaccctaa
    21001 atgtttactt ttgttcgaat tagattgact gttgtccgtt tatatgatag attatcctta
    21061 aattagaggt gaagacaatg ttgaaaaaag aaagattgct tactatcgta gaaatggtca
    21121 ataaaaaagg aatccttacc gttaatgaaa tcatcaatca attagatgtt tctgatatga
    21181 ccgttcgcag agatttagat gaattagaaa aatcgggcaa gctgctgcgt gtccatggag
    21241 gggctcaaag tctttcctac actttagatc aagaactttc tcacactgaa aaagccactc
    21301 tgcagattca agaaaaaaaa gaaattgctg ccacagcagc ctccttgatt cacgagggag
    21361 aaacgatttt cttaggacca ggtacaacaa tcgaactatt ggcgacgcat ttactgaaca
    21421 agaaaattcg gatcattaca aataattatc cagtttttga tattcttgct caagcagatg
    21481 gaccagaaat tctactgatt ggcggtgatt tccggaaaaa taccggcgca tttgtcggac
    21541 cgatcaccaa cgacaattta agaagactaa agtttacaaa agcatttatc agttcaaatg
    21601 gcgttcacaa cgaagcgatc tctacttaca gtaccgaaga aggagaagca cagcagatcg
    21661 ctctaaataa ttcccgtaag aaatatctac tcgttgataa taaaaaattc aatcgagatg
    21721 atttttatgt gttttataat ttgcatgatt tcgatctgct cattacggat cgccagatct
    21781 cgcgggaggt tcaagagcac tatgcacaat atgtcgatct gcaaattgct gattcaaccg
    21841 aaaataccac tataggagcg taactatgaa agggattata tttgatttca acggaacaat
    21901 gtttttggac tctcatctgc atgaagctgc ttggctgcat atgatacatg atcatacaaa
    21961 agatggttta tcagacgagg atattttaaa aaatattcat ggtcggacga atacggagat
    22021 tttgaaacat ttcatttcag agcatttgac cgcctctgag atcgccgtat tgtcagaaga
    22081 aaaagaaagc tattatcgta tgctttgctt gcaaaatgaa aacgagcttc agttgacaaa
    22141 agggcttgaa gcgactcttg accagctgac tgaccaacag attccattaa cgattgccac
    22201 ggctactgtc aaagagaatg ttgcttttta ttttgatatt ttcgatttag cccgctggtt
    22261 tgactgggac aagattgtct atgacgacgg ctctttccct gggaaaccgc aaccggatat
    22321 ttttcttaaa gcggcagaca aactgagctt agctccggct gattgtcttg ttatcgaaga
    22381 tgcttactca ggattacttg ctgccaaacg tgcggggatc ggaacaatta ttgcaattga
    22441 tccattcggt aaaaacaaaa cagtatttga acaggcacag ctaaataaag atggaaccat
    22501 caaagatttt actggttttt ggacagagca cctgtctgcc acatccgtta attcttaaac
    22561 gattcaacta actaccaaag aaaagcaact gaaatgcaca cctcctttta taaaaaactg
    22621 ccagtgaaac gacatgaaag atacactgct atatccttag tgaaatatag cagtgtatct
    22681 ttgacttttt cattctttta accatatgac gcgttcatct ctatctataa ttaacattaa
    22741 aaatagtgtt ttcacattat ttctcaaacc aatcttaaaa atcatcagga gtactttttt
    22801 gatatacttt tattaatgct gggcgtaatg gttaaaagat ttccactagt tcctctccac
    22861 caagctgagt tgaaaaatgc ttatctgtaa acaacataga caaagaggaa ataaacgttt
    22921 ccgtatcttt ctctgtccca aggatattaa ttaattttcc tactaggtaa gtatcattaa
    22981 acttaagatc tgccttcaca tcaaaagttt tgataaaagc ttttatcatg cgtacaattt
    23041 tgtctttatc gccataattg atttcaccac tcatatcaat atagtcacta atccctgaag
    23101 aaataatggc aactgcttct tctttcgcct tatcagataa ttcatttaat gactgggatt
    23161 gattgatgac ttcttgagtt ttatctgtta cgtaatctct cattatccta tcggcatact
    23221 ccgcaaagta atcttgaaaa aattcttcta cagggtaacc aacttcgttt tcccaaccag
    23281 gagaaatttc attatcagta cttattaaat ctat
//
LOCUS       DASJTC010000043        22852 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.043, whole genome shotgun sequence.
ACCESSION   DASJTC010000043 DASJTC010000000
VERSION     DASJTC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.043"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            30..398
                     /locus_tag="U4B19_001487"
     CDS             30..398
                     /locus_tag="U4B19_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEN2077234.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            578..1519
                     /locus_tag="U4B19_001488"
     CDS             578..1519
                     /locus_tag="U4B19_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEN2077235.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1588..2319
                     /locus_tag="U4B19_001489"
     CDS             1588..2319
                     /locus_tag="U4B19_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEN2077236.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2463..4643
                     /locus_tag="U4B19_001490"
     CDS             2463..4643
                     /locus_tag="U4B19_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEN2077237.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQEIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4663..5145
                     /locus_tag="U4B19_001491"
     CDS             4663..5145
                     /locus_tag="U4B19_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEN2077238.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5161..5922
                     /locus_tag="U4B19_001492"
     CDS             5161..5922
                     /locus_tag="U4B19_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEN2077239.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6053..7387
                     /locus_tag="U4B19_001493"
     CDS             6053..7387
                     /locus_tag="U4B19_001493"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEN2077240.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7694..8179
                     /locus_tag="U4B19_001494"
     CDS             7694..8179
                     /locus_tag="U4B19_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEN2077241.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8172..8855
                     /locus_tag="U4B19_001495"
     CDS             8172..8855
                     /locus_tag="U4B19_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEN2077242.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8981..11626
                     /gene="polA"
                     /locus_tag="U4B19_001496"
     CDS             8981..11626
                     /gene="polA"
                     /locus_tag="U4B19_001496"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEN2077243.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11671..12507
                     /gene="mutM"
                     /locus_tag="U4B19_001497"
     CDS             11671..12507
                     /gene="mutM"
                     /locus_tag="U4B19_001497"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEN2077244.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12471..13100
                     /locus_tag="U4B19_001498"
     CDS             12471..13100
                     /locus_tag="U4B19_001498"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEN2077245.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13152..13484
                     /locus_tag="U4B19_001499"
     CDS             13152..13484
                     /locus_tag="U4B19_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEN2077246.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13796..14287
                     /gene="nrdR"
                     /locus_tag="U4B19_001500"
     CDS             13796..14287
                     /gene="nrdR"
                     /locus_tag="U4B19_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEN2077247.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14310..15683
                     /locus_tag="U4B19_001501"
     CDS             14310..15683
                     /locus_tag="U4B19_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN2077248.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15683..16609
                     /gene="dnaI"
                     /locus_tag="U4B19_001502"
     CDS             15683..16609
                     /gene="dnaI"
                     /locus_tag="U4B19_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEN2077249.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16738..17178
                     /locus_tag="U4B19_001503"
     CDS             16738..17178
                     /locus_tag="U4B19_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEN2077250.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17322..18023
                     /locus_tag="U4B19_001504"
     CDS             17322..18023
                     /locus_tag="U4B19_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEN2077251.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18020..19141
                     /locus_tag="U4B19_001505"
     CDS             18020..19141
                     /locus_tag="U4B19_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEN2077252.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19156..20388
                     /gene="pepT"
                     /locus_tag="U4B19_001506"
     CDS             19156..20388
                     /gene="pepT"
                     /locus_tag="U4B19_001506"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEN2077253.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20520..21428
                     /locus_tag="U4B19_001507"
     CDS             20520..21428
                     /locus_tag="U4B19_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEN2077254.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21463..21735
                     /locus_tag="U4B19_001508"
     CDS             21463..21735
                     /locus_tag="U4B19_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEN2077255.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21746..22792
                     /locus_tag="U4B19_001509"
     CDS             21746..22792
                     /locus_tag="U4B19_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEN2077256.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7710 a         3982 c         5017 g         6143 t
ORIGIN      
        1 acaaatgata ttttacgagg aggaaacaga tgtttacgaa tgaaataaaa atcatgcttt
       61 atgtggacga tgtagaaaaa aatgcaacgt tttggagagc agttggcttt gcggaaaaag
      121 atcgtcaaga aatggatggg acgcttataa ttgaagtagg agttgaggac agtcaaacct
      181 catttctgtt atatgatcga gcatttgttg agtcccattc acctggcaca gctactagtg
      241 ccccatcttt gatgttttac agtaaagata tctttggact atataaaaaa atgcagacgc
      301 aatcagtcac attaggtgat atggtacagc ttggagaaga atatgtcttt aactttgctg
      361 atcccgatgg caactacttt gcggttacag gtaaataata gaaataaaga ctttgttttt
      421 ctgtgcgaaa caggaaagcg aagtcttttt aattcaaata ataatattat gtaaacaaaa
      481 aactttccat gcgtaattat acacaagcaa ataaatcgtc aaatgcgaac gccttcttta
      541 tactgtttag gaacgaaaag aaggaaggaa gaaatacatg gattatcaag tactgcttta
      601 ttacaattat acaaaaatcg aagaaccaga ggaatttgcc aaaaatcatt tagccttttg
      661 caaatctttg gatcttaaag gtcgtattct agtagctgaa gaaggaataa atggtactgt
      721 gtctggaacc gttgaaaaca cgactgccta tatgaaagca atgaaagctg atcctcgatt
      781 tgctgatacg atgttcaaga tcgatccaag cgaaggacat gcctttaaga agatgtttgt
      841 aagataccgt ccagagcttg taagtttaaa gctagaagag gacattgatc caaaccaagt
      901 gacaggtgcc tatctttcac cgcaagaatt taaagaagct attttagatg aacagacagt
      961 ggtgattgat gcacggaatg attatgaata tgacttggga cattttcgag gggctgtacg
     1021 cccagatata cgcagttttc gagagcttcc agactggatc cgcgagcaca aagaagaatt
     1081 tatggaaaaa cgggtagtga cctactgtac tggtggcatt cgttgcgaga agttttctgg
     1141 ttggcttgtc cgtgaaggat tcaaagatgt cggacagctg catggcggta ttgctactta
     1201 tggtaaggat cctgaagtcc aaggagaatt atgggacggc aagatgtatg tgtttgatga
     1261 acggatcgct gtcgatatca accaagtaga taaacaaatc gtcggtcgcg actggtttga
     1321 cggaacacca tgtgaacgat atatcaattg tagcaatcct gagtgcaatc gtcagattct
     1381 tgcttccatt gaaaatgaag aaaaacattt aggagcatgt agcatagagt gtgcgaaaca
     1441 tccgcgcaat cgctatgttc aagaccatgg aatctctcca gaagaagcag aactgcggtt
     1501 tacagcttta attaaataga aaacaatagt tttgattgtc gaccatttcc aatatgcgct
     1561 atactagcgt tgaggatggt gatccacatg aaatcattga tttatgctat tggggatgtc
     1621 catggcaaac atgagctttt tgaaaaactt attcactatt atgatccaaa gatccatcag
     1681 cttgttttat taggggatct caatgatcga ggtcctcgta caaaagaatg ttttttatta
     1741 ggaaagcaat tagtcgaaga aacaggagcc atttacctcc ggggaaacca tgaagaatat
     1801 tttttgcaat ttctgcaatc accggaggac tggtatgcgc cttatatgag aaatggtgga
     1861 aaagaaacgt ttgaaagcct gattcaccca ggggcatcgg aggaatactc cccaactgaa
     1921 atctcgatga tgatccaatc gagatattca gaactgatta aatttttagt ccagcgcccc
     1981 ttgtattatg aatgggaaaa ttatcttttt gtccatgccg gtgtggattt gacaaagaaa
     2041 gactggcgta agacaagtct aagagacttt atttggataa gggaaccatt tcatgaaggg
     2101 aaaaacaata caggtaagac aattgtattt ggacatacga ttacaccaat gctccatgga
     2161 gatatgcaga caacagacct ctggattcag gatcataaaa tcggaatgga cggtggggct
     2221 gtattcggag ggtcattgca tggggttgtt tttgacaagg aaggtattct tcaagatatt
     2281 gaatatcaga acaacgaagg accttggcat ggaatctgac taatggaaag aatgaaggag
     2341 ctgtgacttt gttgcggctc tttttatttt tatagagaat attgtttctt tcagtagttt
     2401 aacccaggat aaaaagtatg atagaataaa gaagcaattt ttagcagaaa agaggcattt
     2461 taatgacgga aaatttgaac cagacgatca ttcagcttgt acaaaaagaa ctttcagact
     2521 atcgtcctaa gcagttgact actgtactta atttgttaaa tgaaggaaac actgttcctt
     2581 ttatcgcgcg ttaccgaaaa gaaatgactg gaagtcttga tgaagtccag attcgtgaaa
     2641 tcgaagagcg gtatgcctat cttgaaaact tagaaaaacg taaaactgaa gtgattcggc
     2701 taatcgatga acaagggaaa ctgactcccg agttagaagc agaaatcacc caagccgtga
     2761 aaatgcaaca agtggaagat ttgtatcgtc catacaaaca aaaacgacgg acaaaagcaa
     2821 cgattgctaa agaaaaagga cttgaaccac tcgcgaagtg gctgatgcag cttacagatg
     2881 gagaggtgca atctgaagca gaaaaatata ttgacaaaga aaaagaagtc tcttctgccg
     2941 aagaagcgtt gcacggtgct catgagatta ttgctgaaca agtcagcgat aatgcaaaat
     3001 ttcggacttg gattcggtct tatacgtata acaaaggtat gtatgtcagc aatgtcaagg
     3061 atgagcaagc agatgaaaaa ggcgtatatg aaatgtatta tgactttgca gaacccgtcc
     3121 ataaaatggt ttctcatcga atattagcta ctaatcgtgg agaaaaagaa gatattctga
     3181 aagtattctt tcaagtagat gaagcagcaa ttttagctta cttggatcgc caaatagtca
     3241 aaaatcctgc ctctccttct tcttcattcg tcagagaagc ctatcaggat agttataaac
     3301 gctttattca gccagcaatc gagcgagaac tgcgcaatga attgactgaa aaggcagatg
     3361 agcaagaaat cgctattttc ggcgaaaacc tccgtaatct tttgttgcaa ccgccactaa
     3421 aaggtaaagt tgtcctggga tttgatccgg cttatcgtac tggatgtaaa ctagcagtcg
     3481 tagatgcgac agggaaagtt ttagcaatcg aagttattta tcctcataaa cctgcagcgc
     3541 aagcaaaaag agaagctgct ggaccagctt ttatccaatt aattaataaa tatcaagtag
     3601 atatggtagc tatcggaaat ggtacagcca gtcgggaatc tgaacttttt gtggctgagc
     3661 agttgaaatc cgccgaccat aaaacctatt acgctatcgt caacgaagca ggcgcttctg
     3721 tttactccgc tagcgaaatt gcacgaaaag aatttccgca tctacaagtt gaagaaagaa
     3781 gtgctgtttc tattgcccgt cgcctgcaag atcctttagc tgaattagtc aaaatcgatc
     3841 ctaaagctgt cggagtaggc cagtaccaac atgacgtttc tcaaaaacgt ttggctgaac
     3901 aattggattt tgttgtggaa acagcggtta accaagtagg tgtggatgtg aatacagcaa
     3961 gcccgcagct cttgcagcat atatcaggat tgaataaaac aactgcccaa aacattgtca
     4021 tttatcgtga agaaaacgga gaattcaccg cacgaactca attgaaaaaa gtgccacgct
     4081 taggaccaaa agcatatgaa caagcaatcg gattcttgcg agtccctggt gggaaaaaca
     4141 ttttggacaa cacaggtatc catcccgaaa gttattctat tgcaaaagaa atattaactt
     4201 cagtccaact ttcagaaaaa gagcttggta cagaagaagc agtagaaaaa ttgacacgat
     4261 tatccgtaga gaaactggct gaaagcctat cagtcggaga agaaacgtta gccgatattt
     4321 tagctgggct gacacaacct ggacgagata tgcgtgatga gatgccagcg cccttattac
     4381 gtacagatgt attgagtatg gaagacttga aaccagggat ggaattgact ggaacggtcc
     4441 gtaatgtcat tgacttcggc gcatttgttg atatcggtgt gaagcaagac ggacttgtcc
     4501 atatatcgaa actcagcaaa aaattcgtca agcatcctac agatgtcgtg tcagtaggag
     4561 atatcgtaac agtttggata gaacaagtcg atacgaaaaa aggcagaatc agtttgacga
     4621 tgctttcccc ttatgaagag taacaatgag gagtaacaaa aattgacaca agaagaactg
     4681 cagcagttag tagaagagat atcattaaaa agcttccagc ttcctttcaa gcaccaagca
     4741 atgttcaatc cacgcttacg tacaactggc gggcgctatc atttaaacga tcatcattta
     4801 gattttaatg cgaagctatt tgaagagtca gacgaagaga cgattgcagg aatcatcaaa
     4861 catgaacttt gccattatca tcttcatcta gctggaagag ggtatcagca taaagatatg
     4921 gatttcaagt atcttttgaa aaaaacagga gggttgcgct atgcaccagc cttgacgaag
     4981 aaacagataa agatggagta ttatcgatgc caagattgtt ctgccacgat ctgccgcaag
     5041 agaagaatcg atgtacgcaa atatcgttgt ggaagatgtc atggagaaat caaatggatt
     5101 gagacaaaaa gagaagactt taatgaagca caaaaaatca gatgaaagga tagaaggcct
     5161 ttggaaaaaa gtgagcagag gaatctaaaa gactggaatc agctattaaa ttttttagcc
     5221 gcacctgaag agggaaattt tgaggaactg acggcaccta cacttatttt agctggaaac
     5281 tgtcttccta tcttagctga taaagcagca caaatgtatt taaatggcca agtagatcaa
     5341 ttgtttttgg tcggaggggt ggggcatgcg actagaatcc tttatgagaa ttttgaaaag
     5401 caaggatttc attttgaaga tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa
     5461 gaagtctatg atttgcccga taaggctttt ctgatagaat cgaagtctac aaattctgga
     5521 gagaacgcca ctttttcact ggagattctt cattccttag gagctgtacc agaaaaaatc
     5581 ttattgatga atgatccgac actgcaacgc aggactcgag caacttttga gaaagtatgg
     5641 caaaatgaac aaactatttt cgcgaatgct gtgccatttg tgccggaaat ccttcacttt
     5701 tctgaagaga tcatctttac agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc
     5761 catgcgctag tattaggaga aatggaacgt ctccatgacg atgagaacgg ctatggtccg
     5821 aaaggaaaag attttattcc tcacattgat atatcagagg aagtttggtc ttcttacacc
     5881 agaattaaag agtcaataaa aacggatttt tctcgtactt gaccatttta gcaagcgaat
     5941 cctcctttcc aaataaattt ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt
     6001 gttgaatgta atagctaaaa gaatggacgc tagataagag aagaggattc aaatggaaaa
     6061 tcaaaataaa ttgtatcatt ttgttggtat caaaggttca ggaatgagct ccttggctct
     6121 tgttttacat gaacaaggtc taaatgtcca aggatcagac attgaaaaat atttctttac
     6181 acaaagagat ttagaaaaag caaatatcac gattctgcca tttaacgcag ataatgtaaa
     6241 accagggatg acgatcattg caggaaatgc atttcctgat tcacatgaag aaatccagcg
     6301 agcaaaagaa ttaggtttgg aagtgattcg ctatcacgac tttattggtc attttatcca
     6361 aaattatacg agtatcgctg taacaggctc tcacggaaaa acaagtacga ctggactgct
     6421 ctctcatgta ttgagtggtg tccgtccaac aagttatcta attggagatg gaacaggaca
     6481 tggtgatccg caagcggaat tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct
     6541 tgcctattca ccagactatg caatcatgac caatatcgat tttgatcatc cggattatta
     6601 tacaagtatc gacgatgtat ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc
     6661 tatttttgcg tatggtgacg atgcttattt acgcaaatta aaagcaaatg tgccgattta
     6721 ttattatggt gtcacagaaa acgatgatat ccaagcacga aacatcgaac gcacaacttc
     6781 tggttctgct tttgatgtct atcatgggga tgaatttgtt gggcacttca ctgtaccagc
     6841 attcggtaaa cacaatatct taaatgcttt aggtgttatt gcagtagctt atttcgaaaa
     6901 actggatctg aaagaagttg cagaagaaat gctgactttc ccaggagtaa aacgtcgatt
     6961 cagtgaaaag atcgtagcag acatgaccgt agtcgatgat tatgcgcatc accctgcaga
     7021 gatcaaagca acgattgatg gcgcacgcca aaaatatcca gataaagaaa taattgcagt
     7081 tttccaaccc catacgttta cgcgtacgat tgcattgatg gatgaatttg cagaagcact
     7141 tgatttagct gataaagttt acttatgcga tatttttggt tcggctcggg aagaacaagg
     7201 aaacgttaag attgaagacc ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga
     7261 aaataatgta tctccattgt tagattatca tgatgcagta gtaatcttca tgggtgctgg
     7321 agatgttcaa aaatttgaac aagcgtatga aaaactttta agcagcacga cgaaaaatgt
     7381 actgtaatac aattactgca gtgtccttat aaaaatagaa agagggggag tggcaaaaat
     7441 cttgccaagc cccctttttt gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg
     7501 gtaataagcc caatcaaatc aaaaacatga gaagctgttt tttcttggct gttcttatta
     7561 tttgatgcag gacagctttt tcacgacctc tgtaaactat aggaatctgt taaatcaagg
     7621 gagcagaact tgccttttac gctacttttg gtacaataat gtatgattaa aaataaactg
     7681 gagggtttct atcttgaaca taggtaagcc gagaaaacta ggaaaaacaa taaatgaaat
     7741 cgaggaaggc gactcgttat ccttaacaga atctattgaa gataaccagc ttttgctgta
     7801 tttgggattg acgaatgatg caaatccttt gtatatccaa catgactttg cacaaaaaac
     7861 agaatatcaa cagccaatcg ttccatcagt gatgctgatg gggatcatca caagtgcaat
     7921 ttcaaagcat cttcctggac caggatcaca tgttgtcaat ttttctgtca attttattga
     7981 accggttttc cattatgaaa cactgacttt tcagtttgag gtcataaaag tagataagat
     8041 gaaggatgtg gtgacgattt ctgtagaagc ggagaatacg aacgaagatc gtgtgttgga
     8101 tgctgtcgtt atggtccaac cgccacaagt tcctgaattg gaaatagcaa tcgaaagtga
     8161 aggtgaattg aatgaataat ccaagtacat taatgtcagg tatcaatcgt ttttatgcaa
     8221 aagtttatgg atttttaggt atgggacttg cccttagtgc agtcacttct tacttggctc
     8281 tttgggtatt tcctttgcaa ttagcaactt ttatcaataa ttttccacta ggctttgtgg
     8341 gtctgtggat cgttgaactg attttagtaa tggttctagg cgtaaaagca caaaagaatc
     8401 cgagcttagc aatcggtgga tttattgcct actctctttt gaatggcata acattagcgg
     8461 ttactcttgc atactacgat attggaacag taacgcaagc tttcttgaca gctactttga
     8521 tgttcatggg gatgtctgtt gtcggagtaa caacaaaaag agatcttagc ggggttggaa
     8581 gagcaggaca ggcagcttta atcggagtga tcattgcact agtcttgaac atcttcattt
     8641 tgagaagtag cgtagttgag ctgttcatct ccctactgtt agtcgtcatc tttgcaggga
     8701 ttacagctta tgataaccaa agcatccgta atatctatgt ccagacaaac ggaacagcac
     8761 atagtggaat tgctgtattc atggcattga agctgtattt ggactttatc aacttgttct
     8821 tggcgtttct gagaatcttt ggacggaaca actaaaaaag aaaaaaattc atcagttggg
     8881 gctataccga attatttggt atggtcccgc tttttctttt cgcattccgc aggattcttt
     8941 gttagaatga ataagaactt tacagtaagg agttaagaag atgacaaaaa ataaattatt
     9001 actcgttgat gggaacagtg tggcttttcg cgcattcttt gcgcttcaca attctcttga
     9061 acgtttcaaa aatagaaacg gtcttcatac aaatgcaatc tatgcattca atacgatgtt
     9121 tgaaaatgtt atgcaaaaag agcaaccgac tcatgttctg gttgcgttcg atgcaggaaa
     9181 aacgactttt cggacagaaa tgtatgcgga gtacaaaggt ggaagatcca aaacacctgg
     9241 cgaattcaaa gaacaaatgc catatatacg tgatttgttg acaggactag gggtacagta
     9301 ttacgaattg cccaattatg aagcagatga tattatcggt actttggcag aaaaagtaga
     9361 caaagatcaa tttgatgtcg tcgtgctttc aggtgatcgg gatttgactc aattagctag
     9421 taaagaagta aaagtggata tcactgtaaa aggtgtcagc gatatcgaaa gctatacacc
     9481 agaacatgta gcggaaaaat atgatgggtt gacgccaaaa cagattattg acatgaaggg
     9541 actagccgga gatgcctctg acaatatccc cggtgtgact aaaatcggtg aaaaaacagc
     9601 tatcaagcta ttgaaacaat atggctcagt cgaaggaatt tatgaacata tcgatgaaat
     9661 gaagcaaagc aaaatgaaag aaaatctgat aaatgacaaa gagcaggctt tcttatcgaa
     9721 aaaattggcg accatcaata cttcagcgcc tgtggatgtc tctatcgact cattgaagta
     9781 tgaagggaaa aatctcgata aactcgttcc gttttataaa gaaatggatt tcaatcagtt
     9841 cctttctaaa ttgaatatcg tagaagaaga agtaaaaatg gatgatattt tgtttgaagt
     9901 cgttcatgaa ttcaaagagg aaatgtttac tacggatatg gcgttgtacg tggaaatgat
     9961 gggcgacaat tatcatacag aggaaatcgt cggagtagcg tgggggacag aaaagaaaat
    10021 ttatgtcacg aacgatctat ctgtattcga aaaccgagcg ttccattctt ggatcactga
    10081 tccgactcgg ctgaaaaaag tatatgatgc gaaacgaact tatgttgctt taaaccgcta
    10141 tactggaaaa acgaaaggaa tcgattttga tgtcttgcta ggtgcatacc ttctcgatac
    10201 gaatgaaaaa agcacagata tcgaaggaat agctgctcat tacggataca acgatatcca
    10261 atcagatgaa gctgtatacg gcaaaggggc gaaaaaaggt ttaccagaag aagaggaact
    10321 cttttttgct catttagcgc gaaaagtagc tgcaatcaat gcgttgacac caaaattgag
    10381 ccaagaactg gcagaaaaaa accaagaaga tcttttccgg aaaatggaac tgccattgtc
    10441 tatcattttg gcagagatgg aaataagcgg gatcaaagta gatgcgactc gcttacaaga
    10501 aatgaagggc gagttctctg ctcgcttaag agagatcgaa caaaaaatct acgaagaagc
    10561 cggcgaagaa ttcaatttga attcaccaaa gcaattagga gtgatcttat ttgaaaaaat
    10621 gggattaccc gttatcaaaa aaacaaaaac tggatattcc acagccgtag atgtactgga
    10681 acagcttcgt gaacaagcac caatcgtaga ggatatcctc acctatcgtc aaattgcaaa
    10741 aattcaatct acctatgtcg agggactgct gaaagtgatc caatcagatg gaaaagtgca
    10801 tacccgttat gtccagacat tgacccaaac aggacgatta agctctgttg atcctaattt
    10861 gcagaacatc ccgattcgat tagatgaagg gagaaagata cgtcaggcat ttgttcctag
    10921 agaaaaagac tggctgattt attcttctga ctattcacaa atcgagttac gcgtacttgc
    10981 gcatatctca aatgatgaac atttgaaagc tgccttccta gaaggtcaag atatccatgc
    11041 cagcacagcg atgcgtgtat ttggaatcga aaaggcagag gatgtcacac caaatatgcg
    11101 taggcaggca aaagcagtga attttggtat cgtgtatggc atctctgatt acggactttc
    11161 acaaaatcta ggaatcagcc gaaaagcagc acagcaatac atcgatacgt actttgaaaa
    11221 atacccaggt gtcaaagaat atatggaaag aatcgtacgc gaagcaaaag accaaggcta
    11281 cgtcgaaacg ctgtatcacc gccgtcgata tctgccagac atcaattcaa gaaattacaa
    11341 tatccgttct tttgcggaac gaacagctat caatacacca atccaaggaa gtgctgctga
    11401 tattttgaaa atcgcgatga tcgaattgga taaacgatta aaagaaacag gtcttcaagc
    11461 tacgatgctg ttacaagttc atgatgaact tgtcttcgag gtaccggaaa aagaacttga
    11521 atcattagat aaattggtga aggaagttat ggaacaagct gtttctcttc atgtaccact
    11581 gatcactgac agcagttggg gaaaaacgtg gtacgaagca aaataaacat tgtttaagac
    11641 agagcaatat aatgaaggtg aaaagaaaaa atgccagaat taccagaagt agaaaccgtt
    11701 cgtaaaggat tggaacggtt agtagtagga aaaacgattc aaaaagttca ggtcttgtgg
    11761 cccaagatca tcgagcaacc agagacaccc atctttgagg cttctcttgt tggcgaaacc
    11821 atccaatcca ttggacgtag agggaagttt ttaatttttc atctcgatca ttgtgaactg
    11881 atctcacact tgcgaatgga aggaaaatat caatttacga aagaaaatac accaatcgat
    11941 aaacatacgc atgtactttt cttttttgag gatggaagcc aacttcgcta caacgatgta
    12001 cgaaaatttg gtcgtatgac aatcgtcgaa aaaggagcaa gtgcaactta ccgaggaatc
    12061 atgaaattgg gacctgagcc tttgccagat agcttcttat tagcagattt cgcaaatgga
    12121 ctgaaaaagt cccacaaagc aatcaagcca ttgctgctag accaacgtct agtgactggt
    12181 ctaggtaata tttatgtaga tgaagcatta tgggaagcga agatccatcc agaacaaccg
    12241 gcgaacacac tgcgccccaa agaggtagaa caattacgtc tatcgatcat tgatgttttg
    12301 gatcgtgcaa tagaagccgg aggtaccacg attcgcagtt atttgaatgc attaggagaa
    12361 tcaggcggat ttcaagtagc actgcatgtc tatcagcaaa ctggcaaacc atgtatacgt
    12421 tgtggaacgc cgatcgtgaa aacaaaagtg gcacaaaggg ggacgcacta ttgtccgaat
    12481 tgtcaaaggc taaaaatcgc aaagtaggat ttgttttagg tttgacgggt ggaatagcta
    12541 ccggtaaaag tacggcagcc aaagtctttc agtctcatgg atttcctttg atcgatggcg
    12601 atgtgattgc cagagaaact gttgagcctg gaacgcttgc tctgaggaaa attgtatcag
    12661 tgtttggcca agaggtattg cagcctgatg ggcaactaga tcgagggaaa cttgggatga
    12721 ttgtttttcc atcaaaggaa aaacggcaaa aacttgatca gctattagat ccgtttatcc
    12781 gaaaagcgat aaaagatcaa atcgcctctc taagttccaa tcatccttta gtaattgtag
    12841 atatcccatt gctctacgaa ggtcattatg accactatat ggatgcagta gctgtcgttt
    12901 atactacgcc agaaactcag cttcagcgtt tgatgaaacg taatcaactg acgctagaac
    12961 aagcgcagca gagaattagt agtcaacttc caatcgaaga aaaaaagcag cgtgcggata
    13021 ttttgttcga caacaatgga acaaaagaaa tacttgtggg acaaatcgaa aattggcttt
    13081 ttgctaatcg ctttctttga gtatctgtgt caattttgct atcctgagta tatagaaagt
    13141 gagggatagc gatgggatca ttaaaaaatc aaaaggataa agtcgtaggt aacgcaaaag
    13201 aaaaaatcgg cgagatgctc aataaagaaa atctaatcga tgaaggtcgt acgcaatcag
    13261 aaacagcaga agaaaaagaa aagcagtacc aagcaagaat cgatgaaata gaagaccagc
    13321 aggcagaaga agaagcgaag cggcaagatt tgactcaaga acgaaaaaat ccgacacctg
    13381 atcgtagtaa aacaccgctc ccagaaagtg accacattcc cgatgagcaa aaaatcgagg
    13441 aacagcgaat gaaacactat acaggaatcg aagagaagtt ataaaatagt aaaaaatcga
    13501 gatgtgactt gaagcagatg agaaggtgtt catctgcttc aagttttttg tgctagatga
    13561 ctaaatcagc ttttgtgtaa gttatttctc agtaatcaag gtgatcagta ttggaaaatt
    13621 gaaatttagt aaggacaggg gacacaatta cgcatgaaag tagaaaaaat tgttcttata
    13681 tccgtgtttt ttgggtagga cagtcgcgtt tgtgatttgt ttcgtgttat aatacaacta
    13741 gttgtaaaga aagacacatc ctttatataa aatgcgatgc aagaggtgag acaaaatgag
    13801 atgtcccaga tgtcaacata ataattcacg agtaatcgac agccgtcaag cagatgatgg
    13861 acgtgcgatc cgtcgccgtc gcgaatgtga gaattgttcg ttccgattca ctacatttga
    13921 acgaattgaa gctgctccgt tattagtaat caagaaaaac ggggaacgcg aagagttcaa
    13981 tcgcgataaa attttaagag gactcatccg ttcagcagaa aaaagacctg tagctatgga
    14041 acaaatggaa caaatcgtca accatgtcga aaaccgcgta cgcagtctcg gagaaaacga
    14101 agtatcgacg aatctaattg gtgaatatgt gatggaagat ttagttgatt tagacgaaat
    14161 cgcttatatt cgttttgcaa gtgtttatcg ccagttcaaa gatatgtctg ttttcttaaa
    14221 agagttgcaa gatatcgtgg ataaagcaaa agaaaataat aaagagaata ataaagaaaa
    14281 caattaagaa acaaaaagga ggcatttcct tggaaaatgc ttggaaagag cttcaaccaa
    14341 aaaatattta ccaaggaaga aaagcggctc cgctgagaga agacagcaag aacgccttgc
    14401 tgtatcttta tcagccacta atcggcggag aagccttgag tatctatttc accctgttat
    14461 cagaaatctc tcaagaatct ggcaaaggtc ctgaagggat gcatgcagat ctgcttagta
    14521 gtctaggctg cggtatcccg caattttatg atgcaagaaa aaagctagaa ggaatcggct
    14581 tactggatgt ttattttaaa gaagatagtg atctgggacc tcgttttatt tatgagctgt
    14641 tagaaccact cattcctaaa gcatttttca aagactctgt tctggctttt cttcttctgg
    14701 aaaaagttgg tgaacgacga ttccagaagc ttacccaatt ctttgaacct aaaaggcata
    14761 cagcagaggg atatcaaaaa atcacaaaaa aatttttaga tgtttatcgc ttcaatgaag
    14821 cagtgtacgc tgctaatcaa gaaaacatcg aatcgctgaa taaatctttt gaagaatctg
    14881 aatcagtagc attacttgaa gcagattcga cattcgattg gcagtttttg aatgattggc
    14941 tggcaaaaaa acatattgca agtcttgatg atccaatcat caaacaagtc gacatgtatc
    15001 atcagcttta cggtttcgat gaattgacac ttggcgaact gatcagtcaa tcttatgact
    15061 ttagtgaaca aaaagtatct gaaaaagaac tgcaaaaagc cattttggca atgcagagac
    15121 gtccggtgaa gcaaacaaaa gaaacagttg cggtttctga aaacagtaat gaaccctcgt
    15181 caaacgaaca tttgacaaac gcagcgcttc agctgataaa agaagcacag agcattccac
    15241 cgatgaaata tctggaagca atcaagtcag aaaaaggcgg atatgtttct aaacaagaaa
    15301 actggttact gcaagatctt gtttctcaat caggtctttc cagtagcgtg atcaatgttt
    15361 tgctgaatta tgtactagtg atcaagaata atgctagctt aaatgccagt tatgtaaata
    15421 cagtggcaaa tgaatgggca caaaagaaaa tcgccacagc agaagaagcg atccagcaca
    15481 ttcggcaaat aagtaatcaa gcaaaacaat caaaacaaaa gaagcaaaca aattatcaaa
    15541 ctggcaagcg aaatgttcga cgggaaaagc ttcctgattg ggtcaaccag ccaaaagatg
    15601 aaaaacagat cagcagcgag aaaaaagcag agattgaccg ccgttttaaa gaatacctgg
    15661 caaaaaagga gggtgatatc tagtggaaga cgtaggcaaa gaattgaaga ggatcatcag
    15721 caaacgcaat tatcaagaaa agtatgctga aatgatcgct gaagtcttaa atgatgcgga
    15781 tgttcaagct tttctaagcg cacaccaaga agaattgaca caagcggata ttgaaaaaag
    15841 ttacgctaaa ttgtatgaat tcgtgcaaga aaagcgtaaa tatcaaaaaa acgatccaac
    15901 gatgatcgct cccggctatg agccgaagct gacgttgaat tttcattatg tggatgtcac
    15961 ttatgtacca acggaagcat tgttagcacg gcaaagacaa gaagaaatca gaagccgagt
    16021 aaaagcaatg gatatgccaa aagatattca atcggcttct tttgaaaact ttgaacgtac
    16081 agatggacga ggatatgcga gcttagaagc cctagatttt gtagagaaat atactgctga
    16141 tccaaaaggg tatcataaag gactgtatct agtaggaagt tttggtattg gaaaaacata
    16201 tttattagga gcaacagcga gagaattagc gattgctgga ttcaccacga cgctagtcca
    16261 ttttccgaca tttgccgttg agatgaaaca ggcaatcgga aaagatcaag tggcagaaaa
    16321 gttagatgca gtgaaacgtt cacctatttt gatgatcgat gacattggtg cagatgcgat
    16381 gagtagttgg atacgagacg aagtattcgg tgttatcctt caatatcgga tgcaggaaca
    16441 gttaccaacc ttcttctcat cgaattttac aatggatgaa ttggaacagc atttgtccgt
    16501 cactcaacgt ggagatgaag agccgctaaa agcgaaacgg ataatggaac gcatacgtta
    16561 tttgacaaaa gaaatcgaaa tgactggtcg caaccgacgc aatggttgaa cagttgacaa
    16621 gtccaactga catttgacct agtagtaaaa gcaggtcagc gaatcattat atggacagaa
    16681 atgaccggag acaatcaccg caacaagtaa tgcagaaact taaacggagg attttttatg
    16741 agggctattt tttctagtat atctgtagtt ggtctatttt ttcttttagc aggctgcggt
    16801 agcgcagata gcaacacagg ttctgttgat tcgattgatg cgggtcaaac agtaaaaaca
    16861 agcagtacga aaacggagag acaaagcata tttgtcggaa cgattgaaag tatcactcaa
    16921 gtagaagcag aagaagaaac gcttcagttg aaactcgtcg atgtcatggc caaagaagat
    16981 ccagcgaata tgggaacgtc attcagcaat gacggagtga tcttaaatgc tactgctgaa
    17041 aaaataagcg tggacataaa tgaattgaaa atcggtgaca aagtccaatt tacattagtt
    17101 gagatgccga tcatgacgat gtctatccca ccgcaagttc ctggagattc gattattgat
    17161 gtttcattga acaaataacc aataacattt tttcaaaaat aagaaaatgt tgttttatag
    17221 aacgaaaagg tatatttttt aaggagatat gggaattttt ttattcctag tctccttatt
    17281 ttatttggaa aatgctataa ttatctagaa ggagtgaagc tatgaaccat ttagatttat
    17341 ctcagcgctt agccgcagtg ggtgactttg tccctgaatc cgctcgttta gcagacattg
    17401 gatcagatca cgcttactta cccgttgcat tgatattaag aggaaaaatc gaatatgcag
    17461 ttgctggtga agtcgttcaa ggtccttatg aatcagcacg taagcaagtc aaaaaagatg
    17521 gattagaaga ccgtatcacc gtacgccttg caaatggtct agatgctatc cagccacaag
    17581 atggtattac agctgtcacg attgcgggta tgggaggttc actgatccga gatatcctag
    17641 aagctggcaa gttgaatcaa cgcttaagcg gacaagaacg tttgatcctt cagccaaaca
    17701 tcggagagcg tacattacga tcttggctgc aagaaaatca gtataaaatc caatcagaaa
    17761 aaatacttga agaaaataaa aaaatatatg aaatcattgt agccgaaaaa gaacaaaaac
    17821 cagttactta caatgaaatg gagctgatgt tcgggccatt tctgcttgaa gagaaaagtg
    17881 agatcttcaa atcaaaatgg acaagagagc ttcaacaaag aaaagctatc cgtgaacagc
    17941 tcaaacaagc agctaatcaa gaggcacgtc aaaaacaaat cgagcaggaa atccaaatga
    18001 ttgaggaggt tttaaagcca tgacaatcag cggacgcact ttcatccaaa agtttgaaag
    18061 ctattgtcct cagtggctag ctgaagaagg agacccagtc ggactgcata tcggaacatt
    18121 ggataagcca atccaacgag taatgatgac attagacgtt cgtccagaag tggtcgaaga
    18181 agcaatcaag aaaaaaatcg atttgctgat tgctaaacat cctccaatct ttcgtcctgt
    18241 aaaacggctg gttactgatc agccacaaga aaaaatgtat gcagatttgt tgaaacatga
    18301 tattgccgtc tatgcagcac acacgaacat ggatattatt tgggatggac tgaatgactg
    18361 gttttgtgaa cttctcggta ttgaagtgga gagttattta gtaaagactc atgagattca
    18421 ttataaaaaa ctggccgttt atgtacctgt ggatcatgca cagaaaatgc gtgaagtatt
    18481 agctgcagct ggtgctggta cacaaggtga ttataccggt acaagtttta cctcgattgg
    18541 acacggtcga tttactccgg aagctggtgc acaaccagcg attgggaaag tagggaagac
    18601 agaacaagtt caagaggcaa aagttgaggt tattttgccg gaaactatcg aaaaacaagt
    18661 gattcaagcc atgcggtcag ctcatcctta tgaggaacct gcttatgatc ttttcgctat
    18721 agatgaacca gtcgaaatgt ttgggttagg acgagtaggt gaattgccgc aagaaatttc
    18781 tatagaggca tttgtagaac aagtaaaaga agcgttccaa ctagatggac tgagaatagt
    18841 ccagccaaaa aatgcaaaat cctctgtgaa gcgcattgcg atttgcggtg gaagcggtga
    18901 aaaattttat ccgcaagcaa tcgcccaaag agcagatgtc tatatcacgg gagatatcta
    18961 ttatcatacg gctcatgata tgcaaagcgc tgggttgatt gcaattgatc ccggtcatta
    19021 tattgaatcg ttatgcaagc aacgatttat cgagaaattt gaatcttgga aacaagaaga
    19081 aaattgggat attaactttt tcgtttctga aacaaataca aacccatttc aatttaatta
    19141 aaaggagaga atactatgta cgaaaatctt ttgccacgtt ttttacgata tgtcaaaaca
    19201 gaaacacgct ccgatgcaac aagcaaaacg actccatcga ctcagacaca agttgctttt
    19261 gcgcaagaat tgaaaaaaga attagaagag ttaggcttag ctgatgtttt ctataatgaa
    19321 gataacggat tcgttatggg aacattacca agcaatgttg atcatgaagt gcgttccatt
    19381 ggatttattt cccacatgga tacagcagat tttaatgcag taggagtcaa tccacaaatc
    19441 attgaaaact atgatggcga gtctacgatc gtactagatg cggaagaacg ctatacattg
    19501 aatacaaaag attttccaaa tttgaaaaat tatgaaggcc acacattgat cacaacagat
    19561 ggcagtacat tactgggagc agatgacaaa tcagggattg cggaaatcat gactgcaatg
    19621 gagatcttga tcaataatcc ttctatccct cacggtacca tcaaagtggc ttttggtcct
    19681 gatgaagaaa tcggtgtcgg agcggataaa ttcgatgttg agcaattcga tgtagatttt
    19741 gcttatacag tcgatggtgg tcctttaggt gagttgcagt atgaaacgtt tagtgctgct
    19801 caagcaaata ttacgatcca agggaaaaat gtacatccag ggacggcaaa agatacaatg
    19861 atcaatgctc tacagttagc aatcgatttt cataatcagc ttccagccga tgaagttcca
    19921 gaaaaaacgg aaggatacga aggtttcttc catttgatgg cattggatgg gaatcctgaa
    19981 gaagcttcta tgagttatat cattcgcgat catgatcgtg aaaaattcga agcacgcaaa
    20041 gcacaaatca cacatatcca agaagagttg aatcagcgat ttgatcaaga acgaatcaaa
    20101 gtagatatgt atgatcaata ttacaacatg aaagaaatca ttgaaaaaga tatgagcatc
    20161 gtggaattag cgaaacaagc aatgatcgag cttggtatct ctccagtgat cgaaccagtt
    20221 cgtggaggaa cagatggttc aaaaatctct tacatgggta ttccgacgcc aaatctattt
    20281 gccggaggag aaaatatgca cggacgattc gaatttgttt ctttacaagt aatggaaaaa
    20341 gcaacagacg tgattgtaaa aattgcagaa ttgaacacaa agagttaaaa tcgaaaaaga
    20401 acagcttatg acaaggcgtt cgtcccaatg atggaatgtc atctgtctaa ggctaaaggg
    20461 caggacaatg gaattggttg tcccgccctc ttttttgaag agtaaatagg aggtaaagaa
    20521 tggaaaagct gctagttttt tataacaaaa gttcagggaa agatgagggt gagaaattag
    20581 cacgctggtt tcaagattat gtaaaagaaa agcagccaga attatatgta gaactagctg
    20641 aaactggccc atcgattgat gatgcggaat tagtcaagaa agcagaagcc atagaagctg
    20701 atactttagt aattataggt ggagacggca cgattcatca tatcgtccaa gcgttccaaa
    20761 aaacattatc tcaataccat gtcggattac ttccaggtgg aactgtcaac aaccttgcac
    20821 gtgttcttga gattcccctc aaaaaagaag aagctgctga cgtgatcctg gaaggcaatg
    20881 tacggaaaat agactatggt caagtcaatg atgaagtcat tatctctaca ctgacaatcg
    20941 gaatcttagc agatacagca gtttggatca gtcaagaaga aaaacagcgt tacggctcgt
    21001 ggatctttat caagaagttt tttaagttat tgctgaagaa gaaaaaatat catttggatt
    21061 tagaaacaga cgaggagcat tggaaaggaa aatcccaact gttgacagtc acgatgtcta
    21121 actccgtggg cggttttaca aattttgacg actcagcgac cctagatgat ggaaaatttc
    21181 atgtaacgat cgtaccttcc ttagatattt tccgatttat tttttatttg cctagaatca
    21241 tgagaggcaa attctatacg atccccggca ttacttattt cacagccaaa cgcatgaaaa
    21301 tccaagcgca ggaaaaatct gtgggcactc gaacggatgg agacacgaca gatgatcttc
    21361 cagttgaatt atctgttgta tcaaaaggat taacagttta ttcaccaaag aaagaagaaa
    21421 aagcttagaa atagtagtta acagaaagtt ggaagaagag taatggatgt attgttacac
    21481 aatcctttgt ggttttacct tttcattgtg aatttttact tattttgtct gatggggtat
    21541 gataaacatc aggctagaac tagcggctgg cgtgttcctg agaataattt gatatttgtc
    21601 agcctgttag gcggtggttt gggaggattg atctctcgaa aagttttcca tcataaaact
    21661 cggaaaaaga agtttactat aggattcgct gcaggtgtac ttgttgattt gattctcatt
    21721 tattttttcc attaaggagc aggagatgtc gtctaaagga acgaaaacta aaatcattct
    21781 gaatctgctt ttgctgattg tgatttttgc cattatttat tatttaatca atcaatcatt
    21841 tgcagatatc tttgccgaat tgctgtctac cagtgttgtt gttttggcag cggctgttat
    21901 attagggaca gtttaccaaa tcgctgaagg gcgctcaatc aaagaaattg ctgaaccttt
    21961 cgcgccgaat tttacaacga aagatgggtt cttgacctct agctatattg cgttttaccg
    22021 aatcgtttcg tttggtacag gaacgctgat ctcggagatt tatttttata acaaaaaagg
    22081 aatgaagcta tcccagggag caggggtgac agcactccat atgattatgt acaaactggc
    22141 agtgatcact tatgcattag ctggcttggt catccaattc tctctttttt attctaatgg
    22201 tccatcaatg atctggttta tcatcgctgg tatccttttg acgatcgtga ttgttgcctt
    22261 tttattggcg atgtccgtca gcttgaatgt tcaaatattt tttgtcagct tatcgaataa
    22321 gttatttaaa agtgagcgtt tgcggaaatg ggtggataca tgcaatcagc agatctactc
    22381 tttaagggaa acggtccaaa ccattatcca agacaggact gcgctattgc ggatttatct
    22441 atggaatatg ttaaaacttg tattttggta tgtacttcct tatttgattc tgattgaaaa
    22501 ccatcctaaa atagattttc tactgaccat ttcgtttatc agctttgcag tagtgctttc
    22561 aggggttatt cctacgcctg ctggtattgg ttcctttgaa tttgtctatt tgttattatt
    22621 cagaccagtt gttggaacag ttgacgcagt ttcttcgctt ttactgtatc gtttcagttc
    22681 ctttatcttg ccatttctct atggatttgt gtatgtgatt gctgaacggc gaagagtgat
    22741 caaagaagaa ataatagaag taaaaaaaga gaataaagaa aatcaagggt aggtctttta
    22801 taagatctac cctttttatc aaatcaaaga aaaacgttat ggataagata at
//
LOCUS       DASJTC010000044        22815 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.044, whole genome shotgun sequence.
ACCESSION   DASJTC010000044 DASJTC010000000
VERSION     DASJTC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.044"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(206..1537)
                     /locus_tag="U4B19_001510"
     CDS             complement(206..1537)
                     /locus_tag="U4B19_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2077257.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(1686..2399)
                     /locus_tag="U4B19_001511"
     CDS             complement(1686..2399)
                     /locus_tag="U4B19_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2077258.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            2574..3998
                     /locus_tag="U4B19_001512"
     CDS             2574..3998
                     /locus_tag="U4B19_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2077259.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            4297..5004
                     /locus_tag="U4B19_001513"
     CDS             4297..5004
                     /locus_tag="U4B19_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEN2077260.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            4991..5314
                     /locus_tag="U4B19_001514"
     CDS             4991..5314
                     /locus_tag="U4B19_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEN2077261.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(5403..6440)
                     /locus_tag="U4B19_001515"
     CDS             complement(5403..6440)
                     /locus_tag="U4B19_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2077262.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(6761..7744)
                     /locus_tag="U4B19_001516"
     CDS             complement(6761..7744)
                     /locus_tag="U4B19_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEN2077263.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(8159..8929)
                     /locus_tag="U4B19_001517"
     CDS             complement(8159..8929)
                     /locus_tag="U4B19_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2077264.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(8922..9857)
                     /locus_tag="U4B19_001518"
     CDS             complement(8922..9857)
                     /locus_tag="U4B19_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077265.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(10173..11822)
                     /locus_tag="U4B19_001519"
     CDS             complement(10173..11822)
                     /locus_tag="U4B19_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEN2077266.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(11939..14542)
                     /locus_tag="U4B19_001520"
     CDS             complement(11939..14542)
                     /locus_tag="U4B19_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEN2077267.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(14554..15603)
                     /locus_tag="U4B19_001521"
     CDS             complement(14554..15603)
                     /locus_tag="U4B19_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEN2077268.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(15629..15943)
                     /locus_tag="U4B19_001522"
     CDS             complement(15629..15943)
                     /locus_tag="U4B19_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEN2077269.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(15959..16405)
                     /locus_tag="U4B19_001523"
     CDS             complement(15959..16405)
                     /locus_tag="U4B19_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2077270.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(16406..18328)
                     /locus_tag="U4B19_001524"
     CDS             complement(16406..18328)
                     /locus_tag="U4B19_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEN2077271.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(18540..19436)
                     /locus_tag="U4B19_001525"
     CDS             complement(18540..19436)
                     /locus_tag="U4B19_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2077272.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(19429..20109)
                     /locus_tag="U4B19_001526"
     CDS             complement(19429..20109)
                     /locus_tag="U4B19_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2077273.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(20110..20892)
                     /locus_tag="U4B19_001527"
     CDS             complement(20110..20892)
                     /locus_tag="U4B19_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2077274.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(20885..21802)
                     /locus_tag="U4B19_001528"
     CDS             complement(20885..21802)
                     /locus_tag="U4B19_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2077275.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(21966..22289)
                     /locus_tag="U4B19_001529"
     CDS             complement(21966..22289)
                     /locus_tag="U4B19_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077276.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
BASE COUNT         6844 a         4661 c         3756 g         7554 t
ORIGIN      
        1 accaccaaaa acctatcttt gcatatacta aatagacgta tatacacaga taggtttgtc
       61 ttgtgaaaaa taatgattat ccttaccaaa aaatgtttgt ttttctacat gttcgtactt
      121 atcaaaaaat atgatcctta ttgaaattac tggctattta tattccaacg acttcttgtg
      181 ttaatttttg ttatctcata gtaaattatg tttcattttc acttaattta gttgtcacgt
      241 gttcgtcaat tcgtaaaaac ggtaaatata taaacacact tgcggctata attaatattt
      301 gtaaaacagc tgctcgccag tctccggctg ttgctaaaaa tcccgatata actggtggcg
      361 tagtccaagg aatttgcact actacatgat taattaatcc cacagcagtt gcaaaatatg
      421 ctactgtcaa agaaaaaatc ggtgcaagca caaagggaat aatcagactg atattaaata
      481 caataggcaa tccaaaaata ataggttcgc ttatattgaa tatagcggga gttaccgata
      541 atttagcaaa gtccttataa tcttttctac gactgaagat aaatattgca atcaataaag
      601 caatcgtatt tcccgatcca cccattaccg caaaggtatc tttaaaggcc atattgataa
      661 tatgtggaat ttgctctttg tttgcataag ctgccatatt ttcttgcata ttctgaatca
      721 aaaacggatc taacaatgaa cctgatatta ctgcttgatg gataccaaat ccaaaaaata
      781 gattagcaat agatgtaatt aacaaaaatc ctggtaaacc agttgtaatt ttacttaatg
      841 gctctgtaac tatcgtggta attagcttat ttatatccat attaaacaat tgaatgatta
      901 taaatgacaa taaagcaaac gaggtaatcg taataatgat tgggattaat acattaaaag
      961 actttaaaac agcgggagga atatttccgg atagctgaat ctgtagtttc ttattattag
     1021 aaagtttaat aaataattct gtagccgcta atccaacaaa tatacctaca aacattcctg
     1081 aagctcctgt ataggatata ggtatcacac ccgtcacttc aacagcttta tccgcatttt
     1141 caggagtaaa catcatctgc ataggtgtta atacaacaaa tgtcgaaata gcaactaata
     1201 ccgctgcaat atgatttgta taatttctgc ttgcacacat atgataagat actgctgcta
     1261 caactaataa tgtagttatg cttaatgtac cattggcaat tgaagtacca atttgttgta
     1321 acgtggtcaa tgtactagta tcgacaattt cccccatgaa tcctgttggt tctaagatca
     1381 ctgaattaat aagtgtcaca aatcctgcta acatcataaa tggcataaat gtagcaaacg
     1441 catctcgcaa agttcttaaa tggatctgat tccccaactt agtcgcaact ataccaaatt
     1501 tttcaaatac aatgtccttc ctgctacttt tgcccatatt ttttctcctt tatgcttatt
     1561 gtaatcgatt actttttcca cccaaataat acatcactta tcaaaaaaag taaatacttt
     1621 taattaaata tatatatctt ttttagtata aaaaaataag ctctgaaaaa ataattttcc
     1681 cgagcttatt tttcaacaaa aacagcttca aaaacaaagt cttgatataa atgtctcgat
     1741 accgaatact cgaaaggttt accagacgat agagcaacaa ctttgtctat ctcaatcata
     1801 aattcacctg gatctatttt taatattttt gcgtcaaatt cattcgcagt atctcctttg
     1861 atacgtatgt gcgaagtctt tatttccaga cccagttcat ttgtgatata agcatataca
     1921 gatttcttta aatgttttgg ttccaagcct ggtatgactg ctaatggcat gaatgtttgc
     1981 tcaatcgagt atggttgaca atcaataaaa cggcttcgta caatattata tacatagtct
     2041 tttacactga tacttaattt atcagctact tttttcgatg gaagttcaat tgagaaagta
     2101 tggatttctg aatctacgtg ttcttcacca accacgctaa acaaaccttc gtttggacca
     2161 agaactcgtg agttatcaat tggtgttctg acgacttcag tcccataccc acttctactt
     2221 cgcaaaactc cttcggaaac aagtaagtct acgcctttct ttactgttaa cttgcttgta
     2281 tgataaataa ctgccaattc atctcctgta ggaagtttct caccaatttt atactctcca
     2341 tttacaattt ttttctttat atctaagtaa atttccatat acttcgcaat ttttttcaat
     2401 ggtgaccctc caatcaaaaa catatcttca tctatacttt aacataaata aaccgataaa
     2461 atccacatca aaattataca cacaataaaa aagatatata tatttaaaca aaactattta
     2521 ctttaagttg taagcggtgt tatcattaag tggaaacagg aggtaaaaaa attatgacaa
     2581 ctatgaaaaa gctaactttt ccagatgatt tttggtgggg ttccgcttgg tcagctgagc
     2641 aagcagaagg aaaaggagaa acaggaaaag cagagactat ctgggatcgc tggtttaaag
     2701 aacaacctta ccggttttat caacgaatcg attcctctat tacaaccgat cacatccatc
     2761 aatataaaga agatatagaa ctaatgaaaa aaactgggca taactccttt cgcgtttcta
     2821 tttcttgggc acgaatgttt ccaaatggcg gtaccggaaa agtaaatccc aaagccattt
     2881 ctttctatcg aaaattattt tccgaaatga ataataacgg aatcaaagta tttgccaact
     2941 tatatcattt tgacatgcct gctactcttc aagataaagg aggatgggaa tcacgtgaag
     3001 tagttgatgc ttatgttcat tacgctgaaa cttgttttaa agaatttggt gacttagttt
     3061 gtcattggtt taccttcaat gaaccacttg ggcctatttt aggtacttat cttgaagatt
     3121 ttcattatcc aaacctcatt gactttaaac gcggagcgca agcagcattc ttcactatac
     3181 ttgcacacgc aaaagcaata caagtattta aagaactagc tcttcctagt aaaataggcg
     3241 ttattctaaa tttgagtcct acttttcctc gtagtcaaaa tcctgctgat gtgaaagcag
     3301 cagaaatcgc tgatttattt tatacgcgga gctttttaga tcctatggtt aaagggaatt
     3361 tttctaaaaa gcttatcctg ttattacaag aatataatca gcttcctgaa gattatacga
     3421 aagaagatct tgtatgtatc tacgaaaata ccgctcaaat tcttggacta aactattacg
     3481 agccacgtcg cgtcaaatct cgttcttcta tggtggatac atctagtcca tttttaccag
     3541 agtggttttt cgaaccacat atcatgcctg gacgacgaat gaacgaatac cggggatggg
     3601 aaatttacga aaaaggaatt tatgatttgt gcatggatat caagaaccat tatggaaaca
     3661 tcgaagcatt tatctcggag aacggaatgg gcgttgcgga tgaagaacgt ttcatggatg
     3721 atacagggca aataattgat gattatcgga ttgagtatat caaagatcat cttgcttact
     3781 tatggaaggc aataaatgaa ggatgtaaca ttaaggggta tcatctttgg acatttattg
     3841 attgctggtc atggatcaac gcctataaaa accgatatgg gctggtttca ctcgacctta
     3901 ctactcaaaa aagaacaatt aaaaaaagtg gcgaatttta caagcaatta agtgatacaa
     3961 atggatttag atacgatgta gacttgttag ttgaataata ttctaaaatt ccacttgaag
     4021 tgaacacatt agaaaacaag aataatgaga aataccaata acaaatactt tttcttcata
     4081 cttcatttat acttgtcagt ataagagact gtgacaaatg tagtgatcct cagaagttag
     4141 gtctggaaat ctaacttgag aagatttttt taaactacgt ccagtctttt ttatttgttt
     4201 aaaattgaag ttgacaagtc ttcattaaaa atttagaata ttcacaagtt tgacgacaat
     4261 tatgaaaatt tatgtcgtgc taaaggagtt ttgttattgg atgaaaagtt agatataaaa
     4321 actgcgatca aagacacgtt acctaccgtt tttggttata tcggtattgg acttgcattt
     4381 ggtatcgttg ggaaagctgc cgggtttcat ccgctggtcg tcacgttgat gtccctgctg
     4441 atctatgctg gttctgccca atttatcacg gtcagcatgc ttgctagcca tagtccactg
     4501 ctctccatcg ttctctcgac ctttctagtc aattcccgaa tgattctgat gagtatgacg
     4561 attgctcctt atttcaagaa aaatcggctg cttcaaaatc tgctgattgg cacattgctg
     4621 acagatgaaa gtttcgcact gggaatgaat aaattgaact atactggaca aaagttaaac
     4681 tttcgttgga tgaatacagc taattggatt tcttatttga cgtgggtggg ttcttctctt
     4741 gttggcgcac ttttagggaa ttttattact gatccgaaaa agtttggttt ggatttcgcc
     4801 atcgtcgcta tgtttatcgg gttactttat cttcaagtca tttctgatag aaacacctct
     4861 aaacgattac agttgatcct gattggtctc actttgattc tggtatatgt cggactgatt
     4921 tttatcccaa gtaacttagt gattttagtt gtaacactga ttgggtgcgg attgggggtg
     4981 tggatcaaac atgccttctt ttgagtttac attgctgact attttaggtt gtactttagc
     5041 aacttggctt tctaggatcc taccatttgt cttactgaaa aaattcgatt tgccgcaacc
     5101 gttgcttgag tatttaagtt ttgtcccgat cgtcattatg tctgctttat ggttcagcag
     5161 tctatttacc caaaatatcg gacatctgcc gcagatcaat ctcgaaaatg ctttggcttc
     5221 agttcctaca cttttagctg ctattctttc aaaaagctta ttggtgatcg tcttggtagg
     5281 aattctttcc ctttcgttga ttcgcttatt ataaataaaa aaacaagagc ttatggcaaa
     5341 ttactgtcat gcgctcttgt ttttatcaaa gtcaaataag aaaaaaatcg gcgtattctt
     5401 gcttatccgt tgaacggacc actaacaacg acttttccta atatatgtcc tgtttctacg
     5461 tctttggttg cttgtttcaa gttcttcgca ttcatcccat ctgaatagac cttgctcaat
     5521 gtactgcgga taatcccttc atttgctaaa gcagcaatgt gcgctaaagc ttctccttgc
     5581 gtttcgattt catagttata gtctgtttta gcgaacatat actcccagtc gaagctgata
     5641 gattggtttt tccaaacacc tatatctaac ggctctttga tccctacgat cgttcccaca
     5701 tgtccaaatg gtcggacaag gtgtttgatt tgatcaaagt agcgggtaat atcgaataat
     5761 acagcgacat actctacttg atcgattcct aattcacgaa aacttttact taagtcgtga
     5821 tgataatcga ttggatagtc tacacctgtt tttttcagcc attcaaaatt cttcggactt
     5881 gcagtcgcgt agacttctag gccagcccaa tgcgctagtt gattcaaaat cgaaccgacg
     5941 cccccagctc cgttgatcac taagattttt ttcccgcggt tggcattttc ttctggagtc
     6001 agaccaaatt tttcaaataa caattcataa gcggtcaatg aggtcaacgg caaggctgcg
     6061 gcttcttcat ctgaaagatt ttttggtgca agagcgacaa tacgttcgtc aaccagttgg
     6121 tattcttgat tgcttcctgc tcttgtcgtt gtacctgcgt aaaaaacgcg atcgccgaca
     6181 gaaaattttt gcacttcatc acctacagca acaactttcc cgactccatc aaaccctaag
     6241 atacgtaaag catttcttat cccgttatct tggcgaagtt tcgtatcgac aggattaaca
     6301 gatactgcgt tgacttttac cagaagatcc cgtgaactgg gtacaggtat ctgttctttt
     6361 tcatcaataa agctttttgg atcatcgatc gacaaccctt cataaaaacc gactaccttc
     6421 attaattcct tttctgacat acgaattcct ccttagcttc atcaagttgg agcatccata
     6481 cagttttcat ttcaatatat atagtatgct atttgttcca cttttattca tcataattat
     6541 tctcttactt aaagtaaact atttgcactt ttttggttaa aaaagaggtt gcgacaagac
     6601 ttttgtcaca acctctttta aacggtgttc aatcaactga ccctcggtat tcgctcaaaa
     6661 aacgacaaaa tatgaggagc tatttttgtt tggctgttct tattacttga tgcaggacag
     6721 ctttttcacg acctctaatt cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg
     6781 ttggatgttc tttttcaaag gtgtctaaga tctctcgtac ttcatcagta gatttcgtat
     6841 tcatcaactg aactcgcaag tcatttgctc ctggaaatcc tttgatatag attttaaaga
     6901 aacgatgtag tcctgtgatg gaacgcggga tgatttctga ataatgatct tgcagatcta
     6961 gttgcagacg aagtagtccg atcagttctt tttctgtatg ttccctcggt tctttttcaa
     7021 atgcataagg attcttgaaa atcccccggc caatcatgat gccatctacg ccatattttt
     7081 cggccaattc ttctcctgtc tggcgatctg ggatatctcc gttgatcgtg atcagtgttt
     7141 gcggagcgat ttggtcacgt aaagcaatga ttttagggat gacttcccaa tgtgcatcta
     7201 ctttactcat ctctttacgc gtacgcaaat ggacagaaag attggctata tcttgatgaa
     7261 gtaggtgact gatccattct tccatctcag acatttccgt atagccgata cgcgtcttga
     7321 cgctgacagg caaaccgcca gctttagccg cttcaatcaa ttgtgccgct acttctgggc
     7381 gaaggatcaa gccactgcct tttccacgtt ctgctacatt agggactggg cagcccatgt
     7441 tgatatcgat tccttggaag cccatttctg ccatagcaat gctcatttct ctaaaaaatt
     7501 ctggcttgtc tccccaaata tgggcaacca tcggctgttc atcttctgta aaggtcaatc
     7561 ttcctcgtac actgtctttt ccatctggat gacagaaact gtctgagttt gtaaactcag
     7621 taaagaagac atccggtgct gctgcatgct ttacgacatg gcgaaataca acatctgtta
     7681 catcttccat tggtgctaaa acaaaaaaag gctttggcag ctctttccaa aaattagtag
     7741 tcatttattt gttactctcc attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca
     7801 ttgtcattat actaggaaac aaaaaaagaa gcaaagtgaa cagcttaaaa gatgtttctg
     7861 attgagcaaa aaaagactgt gacaaaaatc ttatcacaat tccttttccg gaataaatgg
     7921 tgttcaaaag cttgcatctg cggtaataag tccaaccaaa cgaaaaatag gaggagctat
     7981 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctccactaa
     8041 acggtgttca atcagctgac cctcggtatt agctcaacaa acgacaaaat atgaggaact
     8101 attttcccgt ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctcttttct
     8161 aacttctcaa tcctcttcct ttattgatca aataccaagt aacggcgtac aaaacaacga
     8221 tgaatagcac aagtacgatc atcgatgttg ctactggcac atcgattgtt ccaaggaatc
     8281 cgtaacggaa accggagatc atataaacga tcggattgac tttcgaaaca gcttgccaaa
     8341 atggcggcaa catagagatg gcatagaaaa cgccacctag ataagttaac ggctgcaaga
     8401 caaatgtagg cacgatcgat acatcgtcat aagactgagc aaaaatcccg ttgagcagtc
     8461 ctgctaacga aaacaagatt gccgtcatca ataatgtaat gatgaccatg aaccatgaat
     8521 acacatgaag tggtacaaag aacaaggaaa tgatcgttac gagagagccg acgagaatac
     8581 tgcgacccac tccgccaatc acaaagcccc aaatgatcac atgcgtagga acaggcgcta
     8641 ccaagatttc ttcgatgttt ttctggaatt tttgagagaa gaaggacgaa gaaacgttgg
     8701 catacgaact agtgatagca gacatcataa tcaaaccagg cacgatgaat tccatatatg
     8761 aaaaaccacc catgtcaccg atccgaccac cgatcatttt tccgaaaata acaaagtaca
     8821 aggatgtcgt gatcacaggc ggaaccagtg tttggaccca gatacgcaaa tagcggttcg
     8881 tttccttgat agctaaactt tttaatgcag taaaataaag attaaacatt tttctcctcc
     8941 gtatgattat tttcttctgt gattttcaaa aacagttctt ctaaacgatt cgacttattc
     9001 ctcatagaaa gaactttgat tccttgtctt gtcagctgat cgaagattcc gttcacccct
     9061 tgatttcgtt caacttcgac agcgattgtc tgttcatctt caaatgtaca agcgtatcct
     9121 tcaatcacag gtttggtctc atatggcgct aaatcaaaga taaatgtttc aaattgcagt
     9181 ttggatagaa gctctttcat actagtattc tcgatcagtt cacctgattg gatgatcccg
     9241 atgttgcgac aaagcatttc agcttcttct aaataatgcg tcgtcaaaat gatcgttgta
     9301 ccattttcgt tcaattctcg gagaaactcc cacatctcgc gtctcaattc aatatcgact
     9361 cctgctgttg gttcatccaa gatcagcaaa cgtggttcat gcatcaatgc cctagcaatc
     9421 atcagtcttc gcttcattcc cccggaaagc atacgtgcac ggacatttct cttttcccaa
     9481 agattggact gcttcaaata tttttcgctg cgtttcatcg cttctttgcg agagacaccg
     9541 tagtagcctg cttgattcac gacgatttgc tggaccgttt caaagggatt gaaattgaac
     9601 tcttgcggga ctaatccaat ctgttgcttt gctcggacta gatccgtatc cagatcataa
     9661 ccgaagacat tcacttttcc agatgtttta ttgactaaag aagtaatgat tccgatcgtt
     9721 gtagacttcc cagctccatt tggtcctaat agcgcataga aatctccttc tttcacggaa
     9781 agatcgattc ctcgcaatgc ttcgacccca cttccataaa cctttttcaa attatttatt
     9841 tcaagcgcat ttgccattta tttcctatct ccttatagac atgattcttt attgtatcat
     9901 ggaatacagc agatgaaaaa acaacttggt tccagcgaaa aatcaggttg ctgggctcag
     9961 gaaaatctcc taatgaaaga catcaatcgg acgaagtcag acaacctttt cagaaaaaac
    10021 aaatcgaaga taaaggatta ctattcatat gtgcaacaaa aggagcgtga gacaaaagta
    10081 aagaacactt ttgtctcacg ctctaaacac gtataaacgg cggaagcaaa agccaagatt
    10141 tttgatccaa taaatacttt cgatcacttc gcctatccca atacatgcca ctgccgcttt
    10201 tcatcaaata acgcggcttc cgtttttgcg gtttctacta aagtctctgg gtaatccaaa
    10261 actttcagca aggcaatcgc atttcgggtc cttgaaggtc cttcttttaa tttgaaatca
    10321 aaactgattc cttttccttc tgttacttgt tcttcaaagt ggacattctc acaataattc
    10381 tttaaaatct ctgtcaattc gatatcatgt gtggcgacaa atgctaaaga tggatactcc
    10441 gcaagccacc gaacgactga agcggaagaa gcaatccgtt cgatcgtatt tgtccctttt
    10501 aaaatctcat ctacgaagca gtagcagcgt tctttggaag ccacttggtc caacaaacgt
    10561 ttgatcgatt tgatttctga aacaaaataa ctgtcgcctt caaataaatc atcttcgatt
    10621 gccatggaag tcaacacatg tccaggttgc agggtaaact gttctgcaat cgctgtttgg
    10681 atcgtctgtg ctagaataca gctgatggct atacttttga catacgtaga ttttcctgat
    10741 gcattggaac ccgtgactaa tgtattcttc tgccagttga ccggattcac taccgcttct
    10801 tttaacaatg gatgatagat atctttcgct gtcacaccgc cctcagtaaa ctctggtcgg
    10861 caagtgatgg gcatataagt acggtaattc aatattgcag cggctacttc taattctccc
    10921 agcaattccc acagcttgat ggcttcagtt gaatgctttt ctaatttagt gagaacgaaa
    10981 tgataggaga taaatggcag catgaacatg atgttgacat attcaaaaat caactctgct
    11041 tcactcccac tcttcatacg aaatgaaaaa cctaatttag ggatgtctct taatggttta
    11101 gtttgctgtt tcagctcttc ttgaagtggt gtcggcagtt ttgctagctg attggcggct
    11161 gaaatcgttg aaaccaaata actcatgcta ttcagctctg tttctagtgt ttgtttcttc
    11221 actagataat agaccgtatt gaatactaac ccaccgattg ccaaatagat tcctagtact
    11281 tgtcctaaaa gaagcaaacc caaacccacc agcggaagca agccaagaaa aataaataaa
    11341 cctacgcgtc caatcttttt actttttcca tccgctagat attgttttgc aaaattatga
    11401 tctttttttc ctaaacgagc gaattgatat tggatttttt ctctcagttg aggattttcc
    11461 tgataaaaag caatcagttt ttctaactgc tgatcttcac caaaatcaaa gctgcgtaaa
    11521 cgttgataca acgcttctga acctacacta gaataggtac tgttgattcc ttcgaatact
    11581 tcgaacatat ccaggtcata ccaagtaaga tcatcgattt cgctatccca tgaacggaac
    11641 ttcttctccg tgagccatgc ttccttcaag ctttcttctt tatcaaatct cggttgataa
    11701 ggaatttttt cccatttgct acgaactgtt tcctttaatt tcaaacgtga aaaaatgtct
    11761 aaagcaacta ataaaataac agctgcaata ataattaaaa cgataaattc tctttgaccc
    11821 atatccctct ccgttccttt atttgttagc tttagtatag cactgttgcg ctatttgaaa
    11881 agagggtaaa aaaacacctt tcaaaataaa aggtgttttt ttcttgtata ttacatattt
    11941 atctctcatt tatcaaaatt gacaaataat catagggagg aattgttgtt aattcttgtt
    12001 tattctccag tgcattgaca actgtcccaa gggaaaggat agcctcaggt actatttttt
    12061 cttgttctgt tggattagct aagcgaagaa caaatccttg ttcatgaaaa taagaaggat
    12121 aaatcgatga tacaattaag tcttcaggta attcaatcaa tgaaaattta cgaggaacat
    12181 caagttcatt ttgtaatggc caaatcttgt tatctaacct gtgaataaat acattcagtt
    12241 tctgcttttg ataagaaata gattgttcta accgtttttc tgcctgtttt gccactttga
    12301 actcattaaa tcttccttct tctacattaa atccaaaagt gactttatac tcacctatct
    12361 cttgagctag cggtgtttcc atcatgacat gcccttcatt tgtcgtatct cctgaagctc
    12421 gcccaggtcg ccaagcaaga ttaggttttc caagttcccc tgttgttgaa aataaggtta
    12481 tgattaattg attttctata cgttcatact cttttaaacc atctgcataa aacgtaaaat
    12541 aatgatcatt ttctgccaca gagacactct tatcgaatat ttctaaatta acaggtttct
    12601 ctaccatagt ttctgaccaa ttcggaggaa tgacatcagg ggtattttca ataaatccgt
    12661 tttgaatttg tgcaattgat ttttcagatt tatctaaaac attaattgct aagcgcatac
    12721 gatgcgagta aacagtatta tgaattttga gtgtgccttc aacgatttga ctttctccca
    12781 gactgaacga tatttcataa gatatttctt ccatttcggg tttttcagat aaacgatctt
    12841 ttaaatcttt aggaagttga gcttttccgt aaacaactaa tgtttcttgt aaagaatctt
    12901 tatatacttt agctgtttct aattttagag atagttctgt atctccttct aatggtgaat
    12961 aatcatacgt atcgccatca tttgctgaat cgatcaaatg aacgaaatca agatattgtc
    13021 tatctccaac tatcaaattc aattgcccat ctttaaaaga aagtttatat ttattgttac
    13081 ttatctcagt tttgtctatc tcgattgctg tttctaattt ttcattattt ttctcaaatt
    13141 caacaacagt gtatcctagt gctggcacat ttacatttat ttttatgtca agttcataat
    13201 aagctggttc atcaaaatat tcgaaacctg tagcaatcat ccgataagca tttttccgtt
    13261 ctggaaaata aacttctttt tcaatgatat tatagggact acctttgaag tgtattttct
    13321 ttgaatcagc tactacatgc acgattttcg atccactata attctcagtc gttgtgttga
    13381 aaacaaggac ttcattcgat tttagcttta atgcatcagc catccgtttt acaataaggt
    13441 tttcgattcc gtcagctatc tcattagctt ctttgattcg atggaaaata tcttccgcaa
    13501 cattatctga aacacagcca ccaatactat catgagcctg gttttgcaaa atctttttcc
    13561 ataaccgacg aagtaggcct ttactgatat ggataccatt tctttctgca atcagcatga
    13621 gcggctcaac tcgttttaga attttttgtt ccaatttaaa attttctatt ttcatttttg
    13681 ttcttactga gccaatagaa cgatgaacac gagagtaaac cggctgacga agttcacctt
    13741 tatattcagg taattctttt ttgtttctga taacttctac ataagaagga taatctgaaa
    13801 taatattgtg gaatttactg tatttattca acttctctac gacttgatca aaatttaaaa
    13861 tcattgactt ttgatctaca cccgaaggta ctaaaatatc tttgtttttt ccttgcatat
    13921 gaagcatttc aactgcttca tccaactttt cttttgcaaa ttcaggaata tctttaattg
    13981 cgtctaaggt catcattcca gtcatataac caaatggaaa attcatggca taaacaaatt
    14041 tatctcccaa tcctttccag ataaaataag gagatggaac catcgtttca aagtttaatc
    14101 ctcgccaaca catgaaatta tcgatttcta cttgatgcaa aagtgtaggc aaattcgcat
    14161 tgaacccaaa cgtgtcaggc aaatacccta ccatcattgg atctccatat ttaaggatcg
    14221 tatcactgat tccaatcatt aagtttctta aaattgattc agcatccact aataaggcat
    14281 ctgtttgtgc ataccacggt cccacgaata gttgtcctgt tttcatcagt cgttggataa
    14341 ccttaagctt ttgcggattg atctccaaat actcatcgac gattgaagtt tgtccatcta
    14401 aacaaaagtt tactttaggg ttcctttcca attcatctag aacttcagta aagacctgat
    14461 cagacaatac gatggcatct tcatttgtaa aataccattc tctgtcccag tgcgtgtgtt
    14521 gaattatatg tgcatttacc atatcagatt ctcctacttt ccttcttcta ctgaatttac
    14581 ttgttgttct tttttatact tgatggattt tagtgcattg accattaacg ctgtaaccac
    14641 tcccccaata acaattgata ctaaatataa aggtatattc gtaacaaaag gaattgccat
    14701 aaaggttgaa agcattgttt cttgagtaac accaaaaaat ccatttagac cacatgctac
    14761 tgcagtgccc accacgatgc ttggtattac tcgtaaagga tctttcacgg caaacgggat
    14821 cgtcccttca gtaatcccca caaggcccat aatcatagct gtctttgcat tatctcgttc
    14881 cgattcatca taaagttttt tgtttaaaat tgttgctaaa aacattccaa atggcggaat
    14941 agcaattcct aataacatgg ctgtactagg tccaaagctt ccgcttgaaa aagaagcaac
    15001 cccaaaagca tatgcgattt tattgatagg tcctcccata tcaaacgcca tcattcctcc
    15061 taaaatagcc gacatcaaag cagcatttgc tcctgacatg ccttctagcc aattagtcag
    15121 tcctgttgtc attgcttgaa taggcgtagc tagtacatac cataatatga ttccaacaac
    15181 taatgtagta acaaaaggaa tcagcataat tgattttaga ctaataactt gcatcggtac
    15241 ttttactttt ttcaatgcat tagctaaata gccacaagct aatccagcaa ccattcctcc
    15301 aataaatccc ataccaagat tttgacacat catcccagtg ataaaagctg gtgctagacc
    15361 gggacggtca gcaattgaat atgcgatata ggctgaaatc atcggtacga taaactgtaa
    15421 tcccgtctga ccaatcattc gcaggactcc ccagaatgta tccgtctggt cccaaacacc
    15481 tgtgccacca aaaatgactg caatagaagt tagtaaccct gctgctacga caagcgggat
    15541 catataggaa accccacttt ggaaatgatt tagaatttct cttccttgtt tcttgaattc
    15601 cattagtaac tcccccacga tatatttttt atttgttaat taatgcaatt gcttcatcaa
    15661 tcgtctcttc accatctttt actacctctg aggtccctac tgttaaaatc ggtttaccgt
    15721 caaagcgacc tcgttctgaa acatttgtat ctactgcaaa tatgacaaca tctgcctcgg
    15781 aaacatccga aattgaaagt ttgttttcta tccccatagc cccctgtgtt tctactttaa
    15841 tatctaaccc tttcttcttc gcaaactttt ctaaattagc ttttgccata taagtatggg
    15901 caatccctgc gatacatgct gttactgcta ctatttttgt catatgaaat tcctcctttt
    15961 aaaatgatag agcttttacc aattcttttt tatttctcga atcttttatt cttttaacaa
    16021 actcgtcatc catcaatctt ccagataatt ctgctaaaat acgtaaatga ttagtacctt
    16081 tatcagtgtc ttttattgct aacaaaaaga cgagatcaac tggttgatca tctaatgcgc
    16141 cccattcaac agaccgcttt aacttggcta cagccactgt tgattcaatt actgctaggc
    16201 ttttgccatg aggaatggcg atatttttcc caattcctgt ggtcatatgg ttttctctgt
    16261 caaatacgtc tttttgaaat tgtttgtggt cagacacaaa cccattagtc tctaacaatt
    16321 tcgataattt ttcgactaca ctttttttat cagatgcatt taaatctatc actatattat
    16381 ttatattgat tttttcactg atcatttata agccttcttt caatatataa actaattttt
    16441 caaacgacga actcgttttt aaatttgcta ataactcatg attcgaagcg atttcattga
    16501 atgtttcata aattttattc aattcaggtt ttactttttc attaagtgca aaaaagaaaa
    16561 cagaatgaac aagtgtccca tcccaatcaa ttccttctgg attaacgtag acacagatta
    16621 ctggcttttt tataaaatct acttcgatat gcggcatagc aaaatcattc atcgctgttg
    16681 aagatagttt ttctcttttg attgctccct ctagtatccc ttctttcgcg taattttctt
    16741 ttaccaattt tttggtaatt atatttaaaa cgttcacttg attatccttt atttggtggg
    16801 taaaaaagca aaatcgttta tctaataaat ttagaaaagc atcttctgtt tgttcattta
    16861 actgtagata ctgttttact tttctgacat ctgcccctaa catcaaagga ctaacaacaa
    16921 taacaggaaa atttgtattt tcaatcggaa tcgtgcttat aacaagttca tcagtgactt
    16981 ttctatcttg tagttcattg atgcttagta catcttctat ctcaaccata tctgcaaaac
    17041 cgttatttat tctttgctct aataattttg atgttccata tccgctacta caaactaata
    17101 tcactttcgt tttgtcaggt ttgtaacgat ccaaaagtga ttgaatatgg agtgttacgt
    17161 aagcgatttc atcttcatta aaatgtatac agaatctctc ttcgatttcc tctaataaat
    17221 ctacacttat cataaatgac cttttaaaag aatatttgat tttgtccgta taagggttat
    17281 agatatttac tcccaatttg agtcttttaa ctgctgcatt gagatgtaat actagattat
    17341 tgatcaattc ttcatcagga taaaattcat ttaatttaat tcttactaac tcttgtagct
    17401 caacacccat atctaaatag gaaagcttcg tttgatctaa gtcgtgattc atagcatttt
    17461 tttcaatcga agtaatgtga atgtcaacat aagcttgttc atctattggc acaataattg
    17521 aaaatgcttt ttcaatcatt ttgattaaaa taagtgtatt ctttgagatt tcttcttcag
    17581 aattattact acgtgataag aaattgcttg tttcaatcct ttctatcgca atagcaaggt
    17641 ggatagctaa agtttgaaat tcataatctg taaatttcaa ttgtgtctta ttcatgtatc
    17701 gattaagcaa ttcggctact tttttcaata tgtccttatt taagaaatta ttcgcttcac
    17761 caatgaaatt gatattcaag tctaagagat ctgttttact gttagatgct gtaaagccac
    17821 cccaataata gttcacaact tctgacatca accttctttt acttttttca tcagctttga
    17881 tgcgcacgcc atttcgatct gaaactaagg acatcccatg cttgtttagt ctttttctaa
    17941 catctttcat tgtgctttct accgtcgacc tgctcacgta taattcgtca gcaagatttt
    18001 gaatctttag agaatcatta gtattcaata atttagcaaa cacataagtt agacggtcct
    18061 ccggagagtc tgcgatattg attttatgac tggataataa atttcgaagt gtttcttttt
    18121 ttccaataag cgaaacgcct tttttaggtt gaaccaacat ttgtacatct ggcatttttt
    18181 ggattatttc tttcaaaatt aaaatatctt tgctaattgt tttcgtagat acatataatt
    18241 gttctgataa ttctttgtaa gttgtgtgct cattttcaat caaaaagtat agtattttca
    18301 tttgccgttc gctaaattcg atgcccatta tcttcacctc ctgaatacag cttaagtgta
    18361 caattattat ctctattttc atacagaaga aagttcttga ttaatgtaga agtttctaat
    18421 attcttgatt atttcaaatc aataaaaaag cctgcaatat ttatatcaca ggcttttttc
    18481 tctattaaat atatataaga taatacattt ttctactagt tctcactaat cttgtataat
    18541 taaaccctcc gcaacttgac ggagatggag aagatgcccc cgtctaatct agcggtcatc
    18601 tctcctccca ttccttccac caattttttt gacaggtaaa gcccgattcc gctagattcc
    18661 gtatttgtgc ggctggtatt ttcggtataa aaacgctgga agactttttc aacagaggtt
    18721 tgcgttcctg gtttgatagg attggccact gttaatcgaa tgaatggatc ctcttttgaa
    18781 aatgtcacct ttgcttgtcc atctgcataa cgcaataaat tctgtatgat attttgcagc
    18841 actcgcagca acatgagcgg atctgctaat acagttgtat ttatcgagtg ttcagcgaac
    18901 gctacctcga tatttttctc agaaaattgt tcataaaaag agaaaaatac ttcttccacc
    18961 tctttagaaa aagaaagcgt atggagatcc atttcttttt gcttcgtctc tattcgagca
    19021 agatcataaa actgatccgt catactgagt aatcttgcaa cagattgttt gatgatcgtt
    19081 aaatacttct cccgtttctc cgtatcctct acctgttcca gtaattgtac ataaccgtta
    19141 atagcagtca aaggagttct gagatcatga gaaaggccga cgatcgatag tctgatgttt
    19201 tcttccattt cttgaaatgc ttgtcctttt tgttcatttt catccaacca tgtattcagt
    19261 gcatctacta ggtccattag attcttttct cgaaattcga ctgaaagacg accaccatat
    19321 tcttgtcgtt cagaaagagt ggcgattttc ttagttaatc tcttgatttc cctttgcagg
    19381 aagaaaataa aacttcccat aaagataaca acgagaagca acagccattt aatcaaatct
    19441 aaaccccact ccccacaccg tatgaatata cacagcatgt gcctttgcta gcttccctcg
    19501 aagattggaa atatgcatat tcactgtttt ctcactatca aaatatggtt cttgccaaac
    19561 tgtttcataa agatttgctc gcgaaaatac ttttttcgga tgttctaaaa aaagcttcag
    19621 cagatcgaat tctcttcctg tcaaatggac agccacacca ttcacattta catccctcga
    19681 ctctttgtcc agtaagattt ccttgtaata gatgcgttgc gacagatctt ttacttggac
    19741 agaagtctga tgacgaagtt ggatgccgat tcgtaacaaa agttcttcga tatcaaatgg
    19801 tttcgtcaca tagtcatccg ctcctgctgc caacaaggaa actttatctc tttgatcgct
    19861 ttttgctgaa agaacgatga tcggaacatt cgatgtctgt cggatttctg ctaacaatgc
    19921 ttcacctgaa agccctggaa gcatgagatc tagaaggatc aaatcgacag gatatgtgct
    19981 taacaataat tttccttccg tacctgaata agcatcaaga agtttgtatc tctgttcaag
    20041 aacctcctcc agtaaggaat gaacagcttc atcgtcttcg ataatcaaaa tagtttccag
    20101 caggaccacc tacttaatgt cagatttttg gaaaataaac attcctagag ctgatgatac
    20161 cacgattgtt actagactga ccagaatcat tttcggtgct tgcgaataag ttgcttgttg
    20221 cgcaaagtcc gtatacatga gccacgtttc cgtaaaccac tccgttagaa attccaagcc
    20281 tttgattttt gcgaaaacac cgggaatgaa ggccgttccc atgatataaa gaaaagaaag
    20341 aagcaaactg acactggtac ttctagtgat gaatgtcaaa gccactggaa cagataatgt
    20401 ccctaatgtc agcagaagca atacaggaaa tacttttaga acataatttc catagttttt
    20461 gattccctca acttcaccaa atccgaaagc aagtgtgtaa gcaagcatgg ctagtcccgt
    20521 gatcaaaacc acgccaagtg ttgtgatcaa aagcgtaacg agaagtttcg acacataata
    20581 agtcattcga ttccgtccta aagataacgt attgcgaatc gtaccgtttt gaaattcttc
    20641 cccaatataa aagcacgcaa atgctgcgaa agccagaaga atccatgcag ccattttttc
    20701 taccattaaa atccctgttg caccgttgat gactgtatcc gttgcttggt ttaattcatt
    20761 gccgtatttt gtatgcatat accattcgtt ccaagaataa aaaagcggaa gtacgaccat
    20821 cagtcctagt gagacccaca ttttacggct cttccatact tttatttttt cagcagttaa
    20881 tagattaaac atgtggattg cctccagtta attgtaagaa ataatcttcc agtttttgcc
    20941 caactaggtg aataccatcc acaggaactt ttgcttctac caatgcttga ttgatggctg
    21001 ctacttggtc aattccttca aaaagctgga taacacggct agattgtaca aaataatccc
    21061 tataaccaag agagtcgaga accgttactg cttgttccgt cgccgaagtg tcaattttta
    21121 taaattgacg agattcttgt gccaattctt ctttcgataa ttctttgatc aagtgtcctt
    21181 catgcaaaat accatagtga gtggcgatct gtgacaattc atctagcaag tggctagaaa
    21241 gcaagacggt gatgccccgt tcttttacca aacgttggat gatttctcgc atttctgcca
    21301 tccctgctgg atccatccca ttcgtcggtt catccaaaat caaaaattct ggattcgtga
    21361 tcagtgcatt tgcaatagct aaacgctggc gcatacctaa agagaaattc tttgctttct
    21421 ttttccctgt attttccaat cgcatcattt tcagcaggtc ttcgatttct ctatccgaca
    21481 caccgccgtt cgctgcttgg atacgtaggt tatctcttgc agttaattct ggataaagag
    21541 caggggtttc aatcgattga cccatacgtc gtcttgctgc ctgcaaatcc gtcgttccaa
    21601 ataattcgat cgaaccttca tcaatcgaga tcaaacccat aatagttctc ataaaggtag
    21661 acttacccgc tccattttcc ccaatcaatc catagatcat tccacgtttg atttcgattg
    21721 atactttatc taatgctttg gcagcaccgt attttttggt tataccagtt gctttcaaga
    21781 ccgtttcatt tccaaaatcc atacttatcc ttctttctta ttgatgagtg aagtatagtt
    21841 gacgggtata aagaaagcag ccagaaatgt ataaagaaag tgtaaatttt ccaattgaat
    21901 gtcaaaaaaa acaatcggac aatacgtgtc tttcctatta tcaggcagtt tgttatacgc
    21961 ttgtatcagt ttcctgattc tttcttttcg accaaagtcg tttctctgta ttcctcttca
    22021 ctgaaaattt ttgcttcttt cagatcaagc ttgagtaatt ttaccagacc ggttataagg
    22081 ataactatag caagactccc tcccagaaaa attagactgt ctcgcagacg tggttgcgtt
    22141 gtcttccaat caaagagcgc accaaacagt gctaatccaa gtggactggc caccatggta
    22201 atcgttgtta tcgttgacat aagtcgtcct tgtatatgcg ttggaacttc tcgctgaagc
    22261 cagaccatat aaaaagcaaa agaaaacatt aatccttaaa aacggactta tgaaaaataa
    22321 tacatgccgt ttgggattta caattagcta ccaaataaga ggttatgaca aaagactttt
    22381 cacaatccct tcttccgaat aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca
    22441 atatttatca aacatgagaa gcaatttttg taaattcctt cttatttctt gaagctgacc
    22501 acttctgtca caacctcaga agcggataaa taatattgtt tgatgatact ttatcttttt
    22561 cccgtttgtt tatagatcga atgatattat agaccaagaa tcctacctat taacatagct
    22621 aaaaacacaa aaaaggcact ttccaaattg tggaaagtgc cagaaaagtt aggattaagg
    22681 taactgcgct cagcattgct tcgcccagta cgattctcgt aactcgtgaa ctcgaacgat
    22741 aatcgcaatt aacgttttga gaactgtgaa gctttacggg ctttcttaag acctggtttt
    22801 ttcttcgtat cattg
//
LOCUS       DASJTC010000045        22798 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.045, whole genome shotgun sequence.
ACCESSION   DASJTC010000045 DASJTC010000000
VERSION     DASJTC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.045"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            179..3457
                     /locus_tag="U4B19_001530"
     CDS             179..3457
                     /locus_tag="U4B19_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEN2077277.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3998..5905)
                     /locus_tag="U4B19_001531"
     CDS             complement(3998..5905)
                     /locus_tag="U4B19_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEN2077278.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6035..6910
                     /locus_tag="U4B19_001532"
     CDS             6035..6910
                     /locus_tag="U4B19_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEN2077279.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6990..7964
                     /locus_tag="U4B19_001533"
     CDS             6990..7964
                     /locus_tag="U4B19_001533"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEN2077280.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8144..9289
                     /locus_tag="U4B19_001534"
     CDS             8144..9289
                     /locus_tag="U4B19_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2077281.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9426..9770
                     /locus_tag="U4B19_001535"
     CDS             9426..9770
                     /locus_tag="U4B19_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEN2077282.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10041..11327
                     /gene="efmA"
                     /locus_tag="U4B19_001536"
     CDS             10041..11327
                     /gene="efmA"
                     /locus_tag="U4B19_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEN2077283.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11465..12064)
                     /locus_tag="U4B19_001537"
     CDS             complement(11465..12064)
                     /locus_tag="U4B19_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEN2077284.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12468..13589
                     /gene="mnmA"
                     /locus_tag="U4B19_001538"
     CDS             12468..13589
                     /gene="mnmA"
                     /locus_tag="U4B19_001538"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEN2077285.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13835..15256
                     /gene="arcD"
                     /locus_tag="U4B19_001539"
     CDS             13835..15256
                     /gene="arcD"
                     /locus_tag="U4B19_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEN2077286.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15270..16388
                     /locus_tag="U4B19_001540"
     CDS             15270..16388
                     /locus_tag="U4B19_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEN2077287.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16448..17740)
                     /locus_tag="U4B19_001541"
     CDS             complement(16448..17740)
                     /locus_tag="U4B19_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2077288.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17872..18450
                     /locus_tag="U4B19_001542"
     CDS             17872..18450
                     /locus_tag="U4B19_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2077289.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18579..18956
                     /locus_tag="U4B19_001543"
     CDS             18579..18956
                     /locus_tag="U4B19_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEN2077290.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18889..20961
                     /locus_tag="U4B19_001544"
     CDS             18889..20961
                     /locus_tag="U4B19_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2077291.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21172..22119)
                     /locus_tag="U4B19_001545"
     CDS             complement(21172..22119)
                     /locus_tag="U4B19_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEN2077292.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22365..>22798
                     /locus_tag="U4B19_001546"
     CDS             22365..>22798
                     /locus_tag="U4B19_001546"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEN2077293.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         7385 a         4154 c         4817 g         6442 t
ORIGIN      
        1 tattttaaaa aaacttattc tttttacgtt aataaatctc tttattatca acaacttaag
       61 ttatattttt tctttttata aaatacactt attataattt tgttttttat tgacagtaaa
      121 aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag gaatacactt
      181 gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc tcagtacccc
      241 ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga acgaagaaaa
      301 tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga
      361 tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa
      421 aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag aaagcacaag
      481 tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt ccacgaataa
      541 cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc aagcaatccc
      601 caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga cctatacgaa
      661 tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc atttgtttgg
      721 gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat atgtctggga
      781 ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc gacttggatt
      841 ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc cagctcctgg
      901 gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg aaaaagaaac
      961 aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca cgaactcgat
     1021 tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat attattacga
     1081 tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa aattatacgc
     1141 ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga cgatcaaggt
     1201 agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc aagaaagacc
     1261 tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag gaaaccaaga
     1321 tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa ataaagcaac
     1381 aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg aatttgatta
     1441 tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc cagataaaaa
     1501 ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga ttcttcatac
     1561 agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag ggtcagatgc
     1621 aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat acgcatatgc
     1681 tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat tgacgattcc
     1741 aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga aaaatccaga
     1801 aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac tgttcagtca
     1861 ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa aaggattaca
     1921 gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg gaaatacagg
     1981 aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc ataacttagt
     2041 caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg atggatttgc
     2101 ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt atcataatgc
     2161 agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat
     2221 caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag aaggtaacgg
     2281 taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga tcaatagttt
     2341 gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata ttatcgtcaa
     2401 tagcagtaca agttttaata acggttcata taatgtggct ctttatacct cttcagcagg
     2461 aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc ttgagatgag
     2521 agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt tgaactacta
     2581 ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat caaaagattg
     2641 gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa
     2701 tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc aaacagaaaa
     2761 ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag aaacctcacc
     2821 gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa aaccaaatga
     2881 acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa
     2941 tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac
     3001 gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg aaacacagtc
     3061 aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag gagaatatac
     3121 gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg ctcatacaga
     3181 atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa
     3241 tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac caaatggaaa
     3301 accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc
     3361 aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg
     3421 tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg
     3481 taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact
     3541 tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta
     3601 cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc
     3661 tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa
     3721 tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt
     3781 ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     3841 actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact
     3901 tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt
     3961 cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc actctttttt
     4021 ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa
     4081 ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta
     4141 aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga
     4201 attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc
     4261 ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag
     4321 gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt
     4381 cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa
     4441 atgcatggga aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg
     4501 attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc
     4561 gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca
     4621 ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat
     4681 ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg
     4741 ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt
     4801 cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca
     4861 tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt
     4921 agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct
     4981 gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga
     5041 attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga
     5101 ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag
     5161 ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca
     5221 gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat
     5281 ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt
     5341 gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag
     5401 ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg
     5461 tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct
     5521 tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg
     5581 gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt
     5641 gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg
     5701 caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg
     5761 tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg
     5821 gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat
     5881 cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat
     5941 tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc
     6001 aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact
     6061 gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc
     6121 cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga
     6181 agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa
     6241 atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat
     6301 cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag
     6361 gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta
     6421 taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct
     6481 tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa
     6541 tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact
     6601 tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa
     6661 tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg
     6721 tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca
     6781 ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca
     6841 cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc
     6901 acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg
     6961 aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga
     7021 agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa
     7081 cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg
     7141 aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg
     7201 attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga
     7261 tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg
     7321 aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac
     7381 tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt
     7441 tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca
     7501 tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt
     7561 tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg
     7621 tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag
     7681 caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct
     7741 gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca
     7801 aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa
     7861 caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac
     7921 cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga
     7981 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
     8041 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
     8101 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
     8161 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
     8221 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
     8281 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
     8341 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
     8401 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
     8461 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
     8521 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
     8581 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
     8641 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
     8701 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
     8761 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
     8821 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
     8881 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
     8941 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     9001 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     9061 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     9121 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga
     9181 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     9241 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     9301 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     9361 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     9421 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     9481 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     9541 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     9601 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     9661 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     9721 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     9781 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     9841 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     9901 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     9961 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
    10021 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
    10081 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
    10141 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
    10201 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
    10261 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
    10321 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
    10381 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
    10441 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
    10501 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
    10561 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
    10621 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
    10681 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
    10741 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
    10801 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
    10861 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
    10921 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
    10981 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
    11041 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
    11101 gtgtcttagg gaacttcaac tctttgatga acctggcagg tccaattgga ttattatttg
    11161 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
    11221 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
    11281 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
    11341 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
    11401 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
    11461 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
    11521 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
    11581 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
    11641 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
    11701 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
    11761 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
    11821 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
    11881 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
    11941 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
    12001 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
    12061 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
    12121 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
    12181 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
    12241 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
    12301 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
    12361 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
    12421 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
    12481 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
    12541 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
    12601 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
    12661 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
    12721 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
    12781 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
    12841 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
    12901 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
    12961 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
    13021 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
    13081 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
    13141 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
    13201 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
    13261 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
    13321 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
    13381 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
    13441 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
    13501 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
    13561 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
    13621 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
    13681 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
    13741 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
    13801 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
    13861 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
    13921 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
    13981 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
    14041 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
    14101 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
    14161 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
    14221 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
    14281 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
    14341 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
    14401 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
    14461 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
    14521 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
    14581 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
    14641 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
    14701 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
    14761 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
    14821 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
    14881 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
    14941 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
    15001 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
    15061 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
    15121 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
    15181 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
    15241 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
    15301 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
    15361 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
    15421 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
    15481 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
    15541 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
    15601 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
    15661 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
    15721 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
    15781 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
    15841 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
    15901 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
    15961 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
    16021 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
    16081 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
    16141 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
    16201 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
    16261 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
    16321 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
    16381 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
    16441 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
    16501 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
    16561 tgaccaaaac aagaagagaa gtcgaaagaa agacgccgcg ataaccaaat gcactggaaa
    16621 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
    16681 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
    16741 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
    16801 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
    16861 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
    16921 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
    16981 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
    17041 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
    17101 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
    17161 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
    17221 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
    17281 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
    17341 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
    17401 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
    17461 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
    17521 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
    17581 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
    17641 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
    17701 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
    17761 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
    17821 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
    17881 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
    17941 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    18001 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    18061 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    18121 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    18181 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    18241 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    18301 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    18361 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    18421 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    18481 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    18541 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    18601 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    18661 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    18721 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    18781 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    18841 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    18901 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    18961 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    19021 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    19081 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    19141 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    19201 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    19261 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    19321 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    19381 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    19441 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    19501 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    19561 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    19621 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    19681 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    19741 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    19801 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    19861 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    19921 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    19981 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    20041 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    20101 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    20161 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    20221 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    20281 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    20341 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    20401 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    20461 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    20521 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    20581 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    20641 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    20701 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    20761 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    20821 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    20881 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    20941 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    21001 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    21061 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    21121 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    21181 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    21241 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    21301 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    21361 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    21421 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    21481 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    21541 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    21601 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    21661 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    21721 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    21781 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    21841 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    21901 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    21961 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    22021 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    22081 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    22141 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    22201 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    22261 tcctcggttt atcaaacttt taagaacaat ttttattagt tggcaagtag ttttggatag
    22321 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    22381 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    22441 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    22501 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    22561 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    22621 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    22681 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    22741 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaatt
//
LOCUS       DASJTC010000046        22274 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.046, whole genome shotgun sequence.
ACCESSION   DASJTC010000046 DASJTC010000000
VERSION     DASJTC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.046"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(128..646)
                     /locus_tag="U4B19_001547"
     CDS             complement(128..646)
                     /locus_tag="U4B19_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN2077294.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(648..1265)
                     /locus_tag="U4B19_001548"
     CDS             complement(648..1265)
                     /locus_tag="U4B19_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEN2077295.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1262..2116)
                     /locus_tag="U4B19_001549"
     CDS             complement(1262..2116)
                     /locus_tag="U4B19_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2077296.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2113..3024)
                     /locus_tag="U4B19_001550"
     CDS             complement(2113..3024)
                     /locus_tag="U4B19_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2077297.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3065..4399)
                     /locus_tag="U4B19_001551"
     CDS             complement(3065..4399)
                     /locus_tag="U4B19_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2077298.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4607..5437
                     /locus_tag="U4B19_001552"
     CDS             4607..5437
                     /locus_tag="U4B19_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEN2077299.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5681..5999)
                     /locus_tag="U4B19_001553"
                     /pseudo
     CDS             complement(5681..5999)
                     /locus_tag="U4B19_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6121..6315)
                     /locus_tag="U4B19_001554"
     CDS             complement(6121..6315)
                     /locus_tag="U4B19_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077300.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6820..7005)
                     /locus_tag="U4B19_001555"
     CDS             complement(6820..7005)
                     /locus_tag="U4B19_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077301.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7855..8196)
                     /locus_tag="U4B19_001556"
     CDS             complement(7855..8196)
                     /locus_tag="U4B19_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2077302.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8196..8426)
                     /locus_tag="U4B19_001557"
     CDS             complement(8196..8426)
                     /locus_tag="U4B19_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2077303.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8713..9051
                     /locus_tag="U4B19_001558"
     CDS             8713..9051
                     /locus_tag="U4B19_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077304.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9256..9495)
                     /locus_tag="U4B19_001559"
     CDS             complement(9256..9495)
                     /locus_tag="U4B19_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN2077305.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(9471..9818)
                     /locus_tag="U4B19_001560"
     CDS             complement(9471..9818)
                     /locus_tag="U4B19_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEN2077306.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(10550..11131)
                     /locus_tag="U4B19_001561"
     CDS             complement(10550..11131)
                     /locus_tag="U4B19_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2077307.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11314..11940)
                     /locus_tag="U4B19_001562"
     CDS             complement(11314..11940)
                     /locus_tag="U4B19_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2077308.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11962..13293)
                     /locus_tag="U4B19_001563"
     CDS             complement(11962..13293)
                     /locus_tag="U4B19_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEN2077309.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(13448..13849)
                     /locus_tag="U4B19_001564"
     CDS             complement(13448..13849)
                     /locus_tag="U4B19_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEN2077310.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(13935..14141)
                     /locus_tag="U4B19_001565"
     CDS             complement(13935..14141)
                     /locus_tag="U4B19_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077311.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(14236..15738)
                     /locus_tag="U4B19_001566"
     CDS             complement(14236..15738)
                     /locus_tag="U4B19_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEN2077312.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(15756..16211)
                     /gene="argR"
                     /locus_tag="U4B19_001567"
     CDS             complement(15756..16211)
                     /gene="argR"
                     /locus_tag="U4B19_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEN2077313.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(16309..16926)
                     /locus_tag="U4B19_001568"
     CDS             complement(16309..16926)
                     /locus_tag="U4B19_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEN2077314.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            17147..18520
                     /locus_tag="U4B19_001569"
     CDS             17147..18520
                     /locus_tag="U4B19_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2077315.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            18595..19707
                     /locus_tag="U4B19_001570"
     CDS             18595..19707
                     /locus_tag="U4B19_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEN2077316.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(19779..21416)
                     /locus_tag="U4B19_001571"
     CDS             complement(19779..21416)
                     /locus_tag="U4B19_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEN2077317.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21584..22192)
                     /locus_tag="U4B19_001572"
     CDS             complement(21584..22192)
                     /locus_tag="U4B19_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2077318.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         6501 a         4537 c         3878 g         7358 t
ORIGIN      
        1 ataacgttct catttaacaa aacttaagtt attaagacaa atgaataaac aactacgctt
       61 tcctcgaaac aaaaaaataa agcacaaatg cgcatttacg cactcatgct ttattcgtac
      121 aattaattca gataacggca gcgcatagga tgccaataac acttcctaaa atagctcctc
      181 ctaaaatgtc agtaggataa tgtaaaagta aaaaaatcct cgaataagcg atcaaacaag
      241 caaataaaaa ggctaatatt cctgcaactg gacttaattc gaacaacgca gctactgata
      301 aaaaggctag cgctgtatgt cctgaaggaa aagaagaagt cttcggttta cttgtgtaaa
      361 ggacaagccg acgatttttt tcataaggtc ttttccgtct tgtcgttttt ttgataaaaa
      421 gcaaaccaac aaaaaaatag atcaaactgc cgataagaat ggctagacca tattctctag
      481 tatcaggtat caagactaaa acagcagcaa ggagcaacca aataaaacca ccattagaga
      541 tattactgat aaatttcaaa aaattgattt ttcgactatc cgttttccgt tgcttggcga
      601 acttgttgat aatactatca tcgattttag caatcaaacg caacatgtta ttcctccttt
      661 ttaagaacct tttcttgcgt acgatggata aaaagcagga cttgaaaata aaacaagaaa
      721 ctgaaaccta ataaagcaaa atagattggt cctgcaaacc agaatgcgac aagaaggatc
      781 aacgtgcctg ccaaatagaa aagcgcgctt ttgaaatcat taaaaaacaa gaaaaatgca
      841 gtaatccaag aagcttcttt ttgttccagt tgaagcaaga ttttgtaact aaagagaagc
      901 gcgcttaata gaagcaaaaa caacgtagta tacatcaatg tatagccaat tcccccaccc
      961 caatagcgga aaatctgata atcgatcaat agaagaagaa aataaacact tgctaaacta
     1021 tcttgctttc cataaagaga gaagttctca cgaaactttt tccagaatag tgaaccgagc
     1081 tttttctctt catttttttt gtaatgataa atcatataca gaactgttcg acttgcagtt
     1141 cctaatgtga aaacaggcaa gacgcacagg aaccatagta gctgtaaatg gataagacgt
     1201 ggaatccatt caatgatttc taagaaataa tcattggctt ttttaccaat cgatcgattc
     1261 atcatccttt tgaacctcct gtcaaatcca tcccttcaat gaaataacgt tgtgcaaaaa
     1321 agaacagcaa aatcgtcggg atcaagacaa gtgttgctcc tgccatcaga taattccatt
     1381 gtgtcgttgc ttggttctga aaaacttgca agccaatctg taacgtgtat ttttcttgat
     1441 cttggatata taacagagga cctaagaaat cattccaagc tgcgttgaag gaattgatcg
     1501 caatggtaat caaggcaggt ttcgtcaatg gaagcatcaa atgactccaa atatacaaat
     1561 gattcgctcc gtcgatttca gcagcttcga tcaattcatt attgatcgat aagtaaaatt
     1621 gacgcatcag aaatatattg aacgcattgc cgaagaacga tggaacgatc aggggcaaat
     1681 aagtacctac ccagccgatc ttggagaata atacatattg agggatcatc gttacaaacc
     1741 ccgggatcat catcgtagaa agaaccaaag caaacatcag ctttttccca ggaaaatcaa
     1801 gcttcgcaaa tccataagca ataaaagaat ttgaaaggac attaccgatc actactagca
     1861 cagtgatgaa taatgtattt cttgcatagc gccaaaaagg aaaggcggca atcgtatcca
     1921 gatagttttc ccatacgatt ttttctggga aaaaggtcgg aggataacga acgatttctt
     1981 ccattggttt caatgaagta aacaccatcc aaagcaatgg aatgatcaaa atgattccta
     2041 ttactgtcat gaggacataa cttagtattt tttttgtttg ccgattcaca ctggtctttt
     2101 tgaaataatc catcatttgc cgtcacctcc agaataatag acccacttag gtgcaagctt
     2161 caaattgaca aacgtcagaa ccaacacgat cagaaacagg atcattgaca tagccatcgc
     2221 ataacccata tcaaaagcta cgaacgcctt gttccacata tgcagattat aaaaaagcaa
     2281 agaatttgca ggtccgcctg ttccattttt cgtcatgaca taagcttctt gaaaaatttg
     2341 gaaagaaccg atcgtagagg tgatcacatc gtaaaaaata ataggagtga ttaatggcaa
     2401 agtaatactt ttgaactttc cccaaacgcc tgctccatcg atttctgctg attcatataa
     2461 agatctagga acattttgta atgtagctaa gtaaagtagc attgctcctc cagaactcca
     2521 tattttcatc aaaatcaacg caggtttcgt ccattgtgga tcaaacagcc aagctggtcc
     2581 agggatatgg aaccagccca gaaatgtgtt gatcaatcct gcagatggag aaagcagctg
     2641 catccataag atgtagactg ctactcctga taagactgcc ggtaaataga aagctgtacg
     2701 gaatacactg attcctttga ttttttgatt gagtactgtg gctaaaaaca cactgaaaat
     2761 cgtcgtcagc ggtacattga agatgacgaa ataaacggtg ttgtataacg atttccaaaa
     2821 taaatcatcg tgaacgaaca ttcttttgaa atttgctaga ccaatgaaat tcatcttgga
     2881 tgtcaaatta taatcggtga aactaccaat aactgaaaaa agcataggcc ctagcataaa
     2941 taccaagaac ccgataataa aaggcgagat aaataaatag gcccatcttg cttctttttt
     3001 ctttcggata gataaacgct tcaaattttc ttgcctcctc tgtacctaga cagtcaaaaa
     3061 tggattattg attttgcttg actaagtttt ctactgcttt ttgtgcatct gccaatcctt
     3121 cttttggtga ttttgttcct aaaagaatct ggtcaatctg ttcattcact aatgtcgtgt
     3181 aatccggagc atctaaagga actggtgtga tcaccgtatt tttaaagttt tcgtccgcca
     3241 tttgatagat catttttcct ttatccgtta ggttgtcatt attcactaaa ttctcattgg
     3301 ctgtcttgct cgccatgata tcaaaacttt tttcgccgaa tttttcttgt acttctggag
     3361 aagaaagata tttgatgaat tcataagagg cctcaggatc ttttgctccg tatggtactt
     3421 ccaagacgaa tccgccaccc cacgaccaat ggcctgaacc ttcttctttt tctgggagct
     3481 gcatcacgcc aaaatcaaaa tcatccggtg cattttccat caaactagag taataattga
     3541 tattttgggc acgcatggct actaatccgg aaataaatgg atcagataca ccagacccaa
     3601 attcggcttc taatttattg atcgtatttt tcccataata atcttgccag tcttggatcc
     3661 actccaaggc ttccacttta tttttagtat caatcttgac tttttcatct ttgtcaaacc
     3721 aactagtccc gttatctgcg tttagcgccc atacatcagc ccctaaattc cataatggat
     3781 aaaaaccgat gcgtgtaaat tcttttcctt cttttttatc tagtttacgt gctgtttctt
     3841 ctaattcatc ccaagttgtc ggtagatctt gttcactgat tccagcttct ttaaataact
     3901 ttttattata aaaaatgact tgtgtatctg tattgaatgg gatcgcataa ggatcacctt
     3961 catattctac tgtatcccaa agctgcggat aaaaatcctt agaagtgtct ttttctacat
     4021 atttggagag attagttgct tgctgtgctt ctgctctttg ggcaacggaa tttatgtcct
     4081 gtacaataac atcgggtgca tttccagctg ttattgcaga taaagattta gtccagatgt
     4141 caccccatgg ctgatactta taatttacat ggatctcgtt ttgtgattga ttgaaatcct
     4201 caatgatttc ttcgatagct tcttttcgtg caccggatcc ccaaaaggac cagaaatcaa
     4261 tattttttct tccatcactt gttttttcag aagaatttgc tgcacttgaa ctggcatcgc
     4321 ttgcattact tgatccgcat cctcctaata aaaacaatgc tgaacttgcc agcaaccctg
     4381 taaacaataa ttttttcata ttttacctcc aaattttaat gtaaccgatg ccggttttaa
     4441 tataacgata tcattttgtt ctgaaaataa caatttataa ggtatgattc taaaaaaatt
     4501 attaatgata ccaatacttt atataattcg cgagcgcttt ctttgaattt tttaatcttt
     4561 cgtagcagaa tgagaatgta ttaaaaatag attaggagga attttgatgt ttagacacca
     4621 gaaagaattg cagtttgaag caaaaccaga tcgtccgaat ccattaatcg cgaaatatct
     4681 gcaggagctg atcggcggcc aatatggcga gatgagtgta gctatgcagt acctgttcca
     4741 aggctggagc tgccgaggag aagaaaaata taaagatatg ctgatggaca ttgcaacaga
     4801 agaaatcggc cacgtggaaa tgttggcgac aatggttgct cgtttattag agggtgcgcc
     4861 tgttgaagat caagaagcag cgatgcaaaa agatccaaca atcggtgcca ttatctctgg
     4921 aatgaatcca cagcacgcga tcgtagatgg cttaggacct cgtcctgctg acagcgtggg
     4981 caatccatgg agcggtgcct atatcattgc gagtggcaac ttgttagcag actttagagc
     5041 taatttaaat gccgaatcac aaggaagact tcaagtagca cgtatttacg aaatgatcga
     5101 tgatcgtggc gtaaaagata tgctgtctgt tctccttgca agagatagct atcatcaaaa
     5161 tctatgggaa tgtgcagtga aagaattaga agaaaaagaa ggttcaatct tagtcccaag
     5221 tactttcccg cgtgacaaag aacgaaaaga cattgcttat gacttctata acttctcaga
     5281 gggagaccaa agtagtgaag gttcttgggc tagtggcaaa gcactggatg gcgaaggcga
     5341 atatcaatgg ttgaaagaac ctaagatcga aggcgctgca ccaaaattag aaccagtaac
     5401 accaaaaatg tatgggacac ctccattcaa gaaataaata tctacccgta gaaacacccg
     5461 ttttttgttc cacacgcttt gtgagcaaaa ctgactaaag tcattaatca taaccaccct
     5521 tcaaaagggt ggttttctct agagctataa gctcaaatca ccggccagcg cctaaagacg
     5581 ctggctttca tattttatgt tcaagccata ttgcctttgc cagttttgcc cacctcttaa
     5641 aaggtttatg tactactcct tagcccttaa aaggatcgtc ttactctcgt acactcagct
     5701 tatctaacgc actatcatgt ttttcttgtt cttgaatgta ctttttgatg gtcgcttcat
     5761 tttgtcctac ttttgtcaca tagtatcctt ccacccaaaa gtgtcgatac ccaaatttat
     5821 attttagatt tgcacaatct cttgatgatc tcctgaatat tggctcgata ttgattataa
     5881 attatttttc ttctatactt tggtgtgaat acaatgtggt atttacacat ctactttgta
     5941 cgggctaaac tatttccctt tattgctcca cccgcatagc aagtggtttt gtatttcaat
     6001 cgctcggcgc gctcaattag gtctaaagac aataaatcta aaaacccgct gcttcactga
     6061 cagaaacaac gggttttcag aaaaaaatga aatgaaaaag atctcttacg gcaatctatt
     6121 ttattttttt gattttttat aaatggaaaa tgcttcaaga agtccaagaa ataaagccgt
     6181 ataaaaacaa gaacctatga aatctttatt tattagatat tcaacagaaa tcccgataac
     6241 actagccaac aatatattta tcagaagtgt aaaataagga tgctttttat agaatagaac
     6301 tagtgttgtt ttcatagact ctcctttctt catatttcca attaatcaca aattcacggc
     6361 attactagaa aaattaaagg ggcgcagaag tggaaaaata ctcttcgact ttccctacaa
     6421 accaggccat cttgtgttta ataactatac caaatctaag gtacctctaa atctaaattc
     6481 catttgtggc ggtgacctcc aatttttaac tcttctatct cccatccagt atacataacc
     6541 ttgatacttc gaccacttat atatcgaaca cacaaatacc tatccattgc aggagtacta
     6601 cctaacttag ttgctacttg catctaaata acagctgaaa agataggact tactcttagt
     6661 aaagtatctg aactattaat ttttaaacta tcatttttat cctcaactac atgcacatct
     6721 gttgcaactt cactttctgt ctcatccaga taaatgatta aggaatcggg aattgcaata
     6781 tcttcagctg acgctggaat agttgagcta aattagtcat catatcttta ttataaaata
     6841 accaacagca aaaaatacaa tgaaaaacaa acttggaact aatgtatcat taataaatgt
     6901 tgaaaaattc agcaactcat tactgtatga ttttagcagc ccaaccaaca tataccctat
     6961 aaaagctaat aagatggtta ggaatccttt agaattttta ctcattcata gacacttcct
     7021 taaataactt ttattataga aggtattgta aataataata ttgacaaaaa gtgactcaaa
     7081 ataaaaaaaa ccttacatta tactggaatg aactgtattg atagtgtacc taaaagctct
     7141 actgacataa gatcgtgtaa tcggtaaagt aattttatta attatacggg atgtaatcaa
     7201 ttagagaata aactaatcat aaaaggtact tttacattca taattgtcgc tttgagattc
     7261 tactttggtt cactatctga cccaattgca cattttcgat cattacaagt attggtgtcg
     7321 ttagaacaac taaataaaaa ctagcggtag atttttcaga atctatgcta atttctgtat
     7381 ggatgttcca gtgatggcaa atttctcttt tcattgtttt tcttgtcgca tgatatgcct
     7441 ttttgtgtta ttcagttaaa ctatagaaat tctttcctag acagatcttg atcccaaatt
     7501 ttacgttttt gagcatcgat atagtttaaa atgacattac tcatatctaa agagtttaga
     7561 ttttttagga ctaaaactgt cagttgttct ttttcttctt tctgttaaag tattgtcgcg
     7621 acttttcgta tacgttaaga tatgtcgtga aaaaacaagt tttgtgacac aaaaaaacga
     7681 atgacttgag gaaatcacct caatcattcg tttaaaatgc tatatattct ttagtttaag
     7741 atacggaaat taatgccatc gaccacacta gtagaatgaa cgacatttac ttggcctttt
     7801 tttaatttcg ttatgtttag catataaaaa tattatagag tttacttaca atgcttaaaa
     7861 catatactgt ataatctgat caattttagc catgtcttgc aatggtaagt tttctatttt
     7921 tttgagtttt cgttccttaa aatcaagaga ttttaattgt gtaataatta cttgtccttt
     7981 tatatctagc tcttcaggta atgagtaacg tgtcggtaaa ttctttatag tggaagtaat
     8041 tggacatatg accgaaaaca ttgttttacg gttaaattca taacgagaga cgactagtcc
     8101 tggtcgtctt ttttgaattt cttttcctac ggagggatca aaatcaatcc atacaatatc
     8161 acctttttta ggaatataaa ttctttcttc tgacactata aaatttctct cccttcagga
     8221 gttaaatctt cccattcgtc tttctcataa tactcggcct cttccccacc actaaatgga
     8281 tcttcaattt ttggaactaa cgtaattgtt ccatcatcag aatacacaac gatatattct
     8341 tggttttctg aaggtttctt tccgttatca gaaggtaaag ttaccactac agaacttcct
     8401 tgtaatctag attttgtaac aagcattatc atcactccct taataaaagt atactatata
     8461 acactaagta acgcaaaatt cccctttgta aactatctag aaaattgttt tgtttaaagt
     8521 atatcttatt ggatataaat agaaataaat tgtagccagt tcattttttc tttaactaaa
     8581 aaaataatcc aagaaacatt gaacctatgg atccaaaagc tacccaaata ctatagctaa
     8641 ccccccgcta ccttcaattt tgcagataaa acgctttggg agaaaatttc aaagacaaca
     8701 ggccatccta tcatgcccga ttataaacaa aaagctggct attccttttg ctctgacaaa
     8761 tttcaattgg gaaagttcac gaaactagca ggtgaagaag tttcaactgt tcggatcaaa
     8821 gaatgcccgc ttcaaataga aacattggtt caacgaattt caactagaca agactttgct
     8881 attgtagaac gtgagatcgt agctattttt gtagaagaag ggattttaca tgatccaact
     8941 catatcaatg tggataaatg gcaacctctt atctataaat tcagagaata tacaactgcc
     9001 aaccagtcgt taactttgaa ttttagattc caagaattca aagagtcata atgataaaca
     9061 tcgttaggta taaataaaaa agactcgcag cacgagtaaa aaacaaatag aagctataca
     9121 ttagaaaggt cgactgtgac cctatagatg cagtatatgc agcttatgag catttcttct
     9181 gttctctttg tttttgggat ctgtgctgaa gtctttttcg ttttttgccg ctctttcttt
     9241 gaaaaatcga ttatgttata ccttctccat tactaattca ggcatctgct gagtaatgat
     9301 ttttcgcgca aaagaaatat ctgttaccca aggttcgatt tgttcttcag tcaagacaag
     9361 cggcattcgg tcatggattg gtgcgactgt gcggttaggt tcagtagtca ttatgattga
     9421 ttctgccatt ttttcatcct gatggctacg ataaaaaccg ccaatataca ctattttttc
     9481 cttatttgaa aacataaatt tgtctttttc ttcattccac tcattgggaa aacacaacga
     9541 ttttgcaaaa atggttgatg aaaggtcttt ttttcttcta ctgtctctgc acgggcatta
     9601 atcatcaatt gtcctttttt aaatccggta aatccccact tcatgatttc cggagtgagg
     9661 atgttttctt cctcttttct taacgtcaca acaagatttg acggaaaaac ttctccagct
     9721 gctaattctg ctgcctgctt cggttgtttc gtctgggctt ctcgataata attctgaagg
     9781 agctctgact ctagatcaaa aaaataacga ccacacatcc taatccctcc caatcttttt
     9841 ttctatcata cactgcaaca agaagttcgc accaacaaaa cgctttcaat ttttgtagaa
     9901 ctaaaaaagt tgtgacaagt gacaaactct ccagtgaatg gtattctcct gccaactcct
     9961 cggtcacaag tcacaatatt caacaaacat gagaagctgc tttttgaaat tgttccttgt
    10021 gactcggagt gaaacggcag gcttacgacc tctgggtaaa cggtgttcaa aagccagttt
    10081 ctcggtatta agccaaattt atgcaaaaat gtagaaagct attttcacaa aatttttctt
    10141 aatactcgaa actaagcgac tttttcacaa cctcttcctt aaacggtgtt caaaagcttg
    10201 catctgcggt aataagccca accaaacgaa aaatatgagc agctattttt atttggctgt
    10261 tcttattact tgatgcaggg cagctttttc acaacctctc cactaaacgg tgttcaatca
    10321 gctaaccttc ggtattagct caaaaaacgg caaaatatga ggaactattt tgccgtttgt
    10381 ttctctaata ctcaggtcaa agcgctgatt tcacaacctc taatacacgg tgttccagaa
    10441 gtagcttctt cgaaaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa
    10501 ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctctaattat taaagaacct
    10561 tttgcgaagt taacaatctt tctttgatca attgaaaatt ctgcctaagt agtgatagat
    10621 cctgtgtgac tttcaacaat aagatacttc cctggatttc agcaatcaac gctttggctt
    10681 gacggtctgc ttcttcatcg gacagttttt cttcagaaag tgccaggata ttttgtttcg
    10741 tatacttgac ccattgattg actaattcta gtagcaacag acggtattct tcatccactg
    10801 tactcaactc aataatcaaa ttacctacag gacagccatg ataagtgatc tgtgtctcat
    10861 gatcagcgaa aatccagtcc aacatttctt ctaatgcttc ttctggctgt tttccagcat
    10921 ttaagatgtc ttcgaacaat tctgtctgcc atacgtccat atgcgcatgg atcaccgcta
    10981 gacaaacttc tttttttgaa gaaaagtaat aataaaattg accttttcct gtatcagaag
    11041 ctgctagaat atctcggatt gacgtttcat gatagccttt tttataaata agatctttgg
    11101 tcgcttccaa gatcttttcc tttgttttca atgatattct cctttttcat ttctcatagt
    11161 cctatattat acagtaaatt catcattttt tcttcttaat cgtttaaaaa agactgtgcc
    11221 aaattatctg acacagccct cactataaaa ctgttctcat atagctagat gttcattgat
    11281 tcaagaactg cgaggacaaa agcaattcct atcttatgat agttgctcta atacttcctc
    11341 tgttgtcatc acttgatctg caataaagcc aaagttcgtc aacgctgcat ccattgcttc
    11401 ttggccagga gcaccaattg catctgtcgc taccacaaca ttgaatccac tctctaccaa
    11461 ttcacgcata tgggaatcgc tacacaagtt tccgttcatt ccaccaagaa ttactgtgtc
    11521 gattccattt tttctcaatt ggagcgccaa atcattgctt tctggtccaa agattttatg
    11581 aggagagctg atgatcgtat tttcatccaa ataaggtttc aattcttcga taaaatcggc
    11641 tcctgaacct tcttccactg attcatattg actttttcta gcaaacatct gctgctcaag
    11701 catcatatgt tctcctgcac cgccaaattg ccatttatgg tcatgtgggt aaaaataatg
    11761 aggagaaata aagagtttgt atcctttttc ttttgcttgt tttaccattt caatcatatt
    11821 ttcgatcgta ttgtattttt ctaagatatc ttttgtcaaa ccaaagccac cgcctgctgg
    11881 tttcaagaac tcgacttgcg ggtcagtgag aactactgct gtatttttat tattgaacat
    11941 ggtggattct ccattcgtta tttattttaa tacatcattt agagcttcac tcattgtcgg
    12001 atgagaatag atttgatctc tcaataacgt ataaggaagt tttccattca tagctagcga
    12061 aatcaagttg atgacttcat gagaatcttc cgcatagata cttgccccta gaatcaagtc
    12121 agtttcaggg tcgactaaaa ctttgaatac acctttagga tcttctaata cttgtgcttt
    12181 agggacaccg cttgtcatat acttgaataa tttgtaattg acgccttgtg ctttggcttg
    12241 tttttctgtt aaacctacat tggataaagt cggagtgata aatacactgt aaggcacagc
    12301 tttccggtta ttcgttgttc gtttattctc gcccttcaac tgatccataa tgattcgata
    12361 atcatctaga gagatataag tgaactgcaa tccgcctttg acgtctccaa tcgcccaaat
    12421 gttgtctgca tctgtacgaa ggtattcatc cacagcgatt gctccgcgat ctgttagttt
    12481 aacatctgta ttttctaatc caagtgatgc tgtacttggt tttcttccag ttgcagccaa
    12541 aacttttgac gcttggattg ttacttcttg gccatcttct gtaaaggtga cggcagctgt
    12601 tgattcttgg tctgctactt gatctacaga tacacctaga tggaattcga ttcccgcatt
    12661 tgtcatgtct tctaaaatca tttgcgcgat atcttcatct tcacgtggaa gaaattcaga
    12721 atgggcatca agaacgacaa ctttagatcc gtattcattg aacattgatg caaattccaa
    12781 gccaatatat ccagccccga tgatcactaa tttttccggt aattgttcct gatccatcgc
    12841 ttgcgtagaa tcaatcaagt agcgacttgt attcaatcct ttgattggta aaatgattgg
    12901 ttcagaacca gtattgatga aaatccgttc gccttcaaca atcacttttt caccattatt
    12961 cgtcacttcg atttgatggt tagaaagaaa acgtgctgta ccgtccatca ctgtcccagt
    13021 tgcctcatcg ctgatcatat gataattttt ggcgcgaagc atgcccgtca atttttcttt
    13081 ccgagcaaca gcctctgtaa agtcgattcc tctttctcca ttgaaaatca atgatttaga
    13141 aggaatacag ccgatgttga tacatgttcc gccatacata cgtgttgact tttcaacgac
    13201 taaaaccgat tcaccttttg aagctaacgc cttcgctaaa gtcttaccgc cctttccaaa
    13261 accgatcaca atattttgat atttttccat catttttccc tcgcttcttt tttacctacc
    13321 gattggtaca aaaatatgat aaccgataat tgtctattct ccaaatcatc tgctttattt
    13381 gtcaaaaaaa accagacaaa aagcggtcct ttcacgtttt tagtctggtt cgatttcctt
    13441 tattcttcta aacaatcgtt acttctacat cgatatttcc tctagttgct ttggagtaag
    13501 ggcagaattc atgcgcatct tctgcaattt tttgggcttc ttcttcgctg atgccatcga
    13561 tatgcacttc taatactaca ccgatctgat agccgccatc atctaaatta taaagagaga
    13621 cacgtgcttt tactgtggac tctgctgtca ttcctgcttc tttgatcata tgttgtaccg
    13681 ccccattgaa acaagaagca taacctgctg caaataattg ttctggattt gttccttctg
    13741 catggattcc tggaggagtg attttcatat gcatcgagcc atttggtgcc tcgacttctc
    13801 cattgcgccc gccatggttg atcattgctg tttcgtatgc ttttttcatt gtactgctcc
    13861 tttcgattga ccatttgcta cttggctata attatagtgt atgacaagta aggcgctttc
    13921 tacaaaaatt ctgcttattc gaataccaga atgccgtctt ctactatttt tccaaaaaac
    13981 aaaaaaatac tactcgtgaa caacagcgtc atatttgttc caaaataaat agcaatcttt
    14041 tctacataat cttttatatg attgatttta agatacatat ataccagaaa gttgacccca
    14101 taaaccaata ctgttaaaaa aataaccata ttcgttacca ttttttcctc cttttgctgg
    14161 tctccctacc agcactcgat ttaaccatac cacttttttt gccgtccaag caactagcct
    14221 ttcaaaaaga ctggtttatt ctgctgttaa tgattcaaga tcagaaaaat gtccgaataa
    14281 ttttgctaat tttactaacg ccaacttgtt ctttccgaat tcatcatttt ctatctgtaa
    14341 tactagtaac ggctcatgta agcttaaacg gatcaaaaac cagccgtttc cgtatggctc
    14401 ttctagatca tatcgaatac cttcttcatt ttccgggtcg aagttcatcc ctttttcttc
    14461 ttccaacagt cgcttcattt cttctaatat cttgtttcct tgttcgcgtg gttcatcgct
    14521 gaacaatttt aatcggattt cttgtacttc tacgggctgt ttcaactctt tgatttgttc
    14581 atctaatgtg ataccttttc tttttaattc tggcaaaagc atcaggatct ttgcgatgac
    14641 atatgcacca tcatccagaa aatagttttc cttgaatgca gcgtgtccgc ttgtttcgat
    14701 ggctagctgt gtatctttcc caagacggtt aagttctaat gctttattga ttacatttcg
    14761 atagccggaa atataacgaa cttggatgcc tcctttttct tcaatgaatc tttgtagatg
    14821 agaagaagta ggggaattcg tcacgattct tgttcctgga tgctctttta atacgattgt
    14881 acttaatact gcgatcaaat tatttcgatt gatcactgtt ccagaagcac tgacaactgc
    14941 agaacgatcc acatctgtat cgaaaatgat cccaagatct gcttcatttg ctaaaacagc
    15001 ctgcttgata ctttccatcg cttctttatt atcaggattt gggatatgat taggaaatcg
    15061 gccatctggt tccaaaaatt gggaacctgt tgaatccgca cctaagctat tcaatacttt
    15121 ttcagcaaag aatccaccag caccatttcc ggcatcaaca atgattttcc aaccatttag
    15181 tggtttgctt tctagtgttc caatcccctt tttaattttt gtgactaaat cttgcgcata
    15241 aggtgtcaaa aggtctgctt tttctacgct tccagtttgt tcgcttttta ttttttgctg
    15301 ctgtgatagg ataaaagcaa tatcttcttt ttctgctcct ccttctgagg taaagaattt
    15361 tattccatta taataatagg gaagatggct agcagtcagc ataaccgcag catcgcacga
    15421 gaactgttca aattgagtgg acataaacat agcaggcgta gtagctaatt cgaaatcgat
    15481 gacatgtatc ccttgttctt tcagactttg gatcaacgcc tctttcagtg ctggtccgct
    15541 tatcctactg tcatgaccaa tcccaatcgt ccatttttta tttggatcgg ttgatttctt
    15601 ctttttcttt atccagtttg ctagtcccca gccgatcttt tttaccatgt ctgtcgtcaa
    15661 gtttacttca tatccttcaa ctggtaatgc gattcctcga atatccgatc cattttgcag
    15721 cgctttcata tctttcatat aaaagtcctc ctcatttatt ccctcatttt ttccagcttt
    15781 tctgcaaatc gctgtgcttc ttcttctgag cggcaaataa taatgaatgt atctacaccg
    15841 gccatcgttc cgacaacttc agggtaagcc acttcatcta gccaattcgt taccacatcg
    15901 gcaccatttc gttcagtcaa tacgataacc atgaactgaa cagactggat acgcaaaacc
    15961 tcgcgtctaa ttgctgtacg caaacgttct tcatttaaag aaacagatgg ctggttaaat
    16021 aatgtatagc gtactatttt gttttcattt tgacttttta ctaacttcat ttctcgaatg
    16081 tctcgagaaa tcgttgcttg agtcacatcg gcgcctgctt catgcagccg cgcaagcagc
    16141 tcttcctgtg tcccaatagg ttgttctgta atgatctggc ggatcagtgc ttgtcgctgt
    16201 ttctttttca tattagacac ctctcaatgt tgattatagc aaaagataag taatatttct
    16261 tcatattttg gtatttttta cttttatttt ttgttttcat tcaggttgct aacctttttc
    16321 tattttaaca cgcaaaatcg tcttcatttt ttcaggtgct gattttcttg gatcgctgat
    16381 gtaaatctct ttgtgttctt tagaagctct ttcatagcct tcttttgcga tgaatgcttc
    16441 taatttctca aacgttgctg gttcttcggc atagggcccg acgtgtaaga tttgggcaca
    16501 aagaccttct tccatctttt tgaatactac ttgttctttc aagtcttccg gaagtttgct
    16561 agaacgctga atggcttctt gtgctatcgc ttctgtaaca aagtcaggct gtcggatcat
    16621 caattgataa gaaaaatgtt cttttttcat cactggttca tgcaaatact tttcttgcag
    16681 tccccattgt ccttccagtg gatagacagt atacatctga taatcaggaa tcagccaatt
    16741 ttttttaggc gacatgcgta ttgcatagct gactgcatat agacaggcta ctcttcgttt
    16801 aaagtcttcc tgattcgggt cgccgatccc tctgatgcta aagaatgatt ttttaggaac
    16861 ctctagtatt tgcgggcttt ttttcatact atagctcttg tctttacgcc attcaatttt
    16921 ttccattttt cttcctccgc gaaattccat ttagttattt attatcatac ctctttttga
    16981 gtgacttggc taatcgtgtt gacactattt ttcagtttgt gctagaataa gtgaagtaaa
    17041 taaatcgtac agataacacg tttcggcgtg tcgagagaga ggcggtttct atttttataa
    17101 tagaaatcac tctctttttt tacgaaattt tttagaggtg atgtaaatga caaaacaaaa
    17161 acagctgctt ttagtacctg cagtaattgc aacagggatg atgtcttttg cgggcgtatt
    17221 gatcgagacg gccatgaatg taactttccc aactcttatc agacagtttg atttaacgac
    17281 tgcacaagtc caatgggtaa caacgattta tcttttgatg atttcgatca ttgttccgct
    17341 gtctacttat ttgaatcgca attttagtct caaaaaactc tttttagctt caaatctgct
    17401 tttcttggca ggtataatga tcgatttctt ctctcctacc ttcatggtcc tattgtttgg
    17461 tcgattgctc caaggtgcag cgacaggtat cgctcttccg ttgatgttcc atattatctt
    17521 gacatttgcc cccctcaatc gacgaggcgc gatgatgggt gtcggtactt tgacaacggc
    17581 cattgctcca gcaattggcc ccacttatgg aggcattatg acttcacatt ttacatggaa
    17641 tcatatcttc ctgtttcttg tgcccgtttt gtttctttct ttgattatcg gctggtttgc
    17701 tattcccgag atggcagtac aaaaaagtgg ttatttggat tggataagcg ttttaggaat
    17761 ttgtcttttg tttagtggaa gtctaacatt tttgagtacg ttgggaacag tttccagctg
    17821 gattgcttta atcatcggcg gtattggctg gttccttttt tactatcgaa gtaaaaaagt
    17881 ggagcatcca ctgatccgac tgtctattct ttcaaataaa acattccgct tgtttttgat
    17941 tggcttctta gtttatcagt ttttattact tggtgtatct tttgtcttgc cgaatttcct
    18001 tcagatcgtt caaggaagta gtccttttgt cgctggatta gcgatgcttc ccggcgcagc
    18061 ggttggtgct ttgctttctc ctttttccgg gaaaatgctg gatcaatatg gtccgaagaa
    18121 gccaattttt gctgggctga ttctttcttt gattggttgg ctagcattat tcctactcat
    18181 aggacacgct tcagtgggtg tattgattgc ttgtcatgtg ttttatatga tcggtatcgg
    18241 attatcttac agcaatatga tgaccaccgg aatgaatgca ctgaatgaag aactacaagg
    18301 agacgggaac gcaatgttca ataccttgca gcagttttct ggcgctgtgg ctacttcatt
    18361 agtagcggtc attatcaatt acgtccagca tcatacttcc cataactatg aagtgtctac
    18421 tacattagga tcaaaagcag cattaggcgt cttgttgctg ttgcttctcg taagctttgc
    18481 tcgattcgcc tattatctgt tctttgctaa aaaagcgtag attccatata cggcaggtgt
    18541 tgcatctgcc gtttttttgt gtgaaaatgg ttgtttgtaa aaggaggctt ttttatggaa
    18601 aaaattgcca ttatcggtgg cgggatcatt ggtatgaccc tagccagtta cttagataca
    18661 tcaaaattca atgttgcttt atttgatgaa gggacaggac aggcaactaa agctagcgct
    18721 gggattatct ccccttggtt gtcgaaacgc agaaacaaac aatggtatcg tctggctaaa
    18781 gacggcgctg cttttttccc taaacttata aaagatttcc aacttgatca aacgatttac
    18841 gcacaatcag ggacactttt gcttcgacct gaaaaagatt tagctgaatt ggctgttctt
    18901 gccgagaaac gaaaagaaga agcgccagaa attggaaaca tcgaatggct ttcacctgaa
    18961 cagacatctc aagcacttcc gttattgaaa ccagcagttt cactgcgtat cagtggtggt
    19021 ggtcgtctgg atggaaaagc atatttagaa catcttggtc agcgtttagc acaaaaacaa
    19081 atggagcaga tccaggcaaa agtcactttg cataagaaga gcgattcttg gctcatcaag
    19141 ggaccgtttg gagaatacac agctgatcgt gtaattttga cacctggccc ggcacttaag
    19201 cctttattac aagaattagg ctatgctgca gatatacgtc cgcaaaaagg gcagctctta
    19261 gcatttcaga catcttataa gatgagtcaa gactggcctg ttgctatgct tgacggagaa
    19321 gctgatttca ttcctttcat ggatggaaag atccttttag gtgctaccca tgaaaacgat
    19381 caaggctggg atttggctcc tactagcgaa gcttataagg gattgactaa gagtacagaa
    19441 cagttcttga ctgatccaga tcagataaaa aactggccat acgagttccg tgtaggtacc
    19501 cgtgcttata cgtcagattt ttccccattt ttcggcgtac tgccagatga tccaagctta
    19561 ctggctgcta gcggtctagg ctcttctggc ttgaccacag gtccttatat cggctatcta
    19621 ttagccaact tcttaaatac tggtcattgg cctacggaag attatcaaaa ggacatctcc
    19681 acctatatta cgaaaaatcc atcatagaaa aacagctttt tcaagtaagg atcccgagcg
    19741 atattgcgca tggattactt cttgaaaaag ctgttatttt agttcaattt ctgaatcaaa
    19801 ttctccaaac tttcttttgc ttcatctggc aagagatctt ttcctatctg agcttcaatg
    19861 aatcgtaaac gaatatcaag tcgttgtttc accagatttt tataggacgt atcctcaaaa
    19921 gaaacaggaa aatctggtat aggtaaatag tggatgtctt caaatggacc aaacgacttg
    19981 aattctgccg tttgttgcac gattctttca acgattgaca atgtcgtttc aggtttgatt
    20041 tttttatcat cgaaatatcc agtattcagg acatagcagg ctgtttgctg tttcaaaaac
    20101 aactctcgaa aagcttgata gtcttcccta agaggataga cacggaaagg attagcaaat
    20161 ggttcaatga ctagctcatc tagctgtctt tttgtagtga tattttccgc gtctgatcgt
    20221 tttgttgcta atgttgcacc aaaagacgct gctaaaactg ggtcattgat cttgataatt
    20281 ggcggaagcg aatcatcttt catgatccaa aaaacagcat cgatcttttc gctaagatga
    20341 tcgactcggt cgggtgtcgc ataacgtgat ttgactgtcc gcccatttcc gtttcgtata
    20401 tctcctgtta ctaggacttt ttctccttta cttgtacatg tcacgcccac attctgacaa
    20461 gtaaggaaag aagaaatggc cggatcatca gacggatagt cttgtgtttt atcaaagtaa
    20521 gaaggttcca aggcagtagc aaaaccattt tttcgagaaa taacgaaagc atcgtcatgc
    20581 aagatcgaaa cagagtgacc ttccgtatgt tttgctaagg taatcgttga ttttccagaa
    20641 ccggacaacc caaatacagc catcgtatag gtctggtcat tactagcata ttgtttcatc
    20701 ccaccgtggc aggcaataaa gccattgcga tttcccaatg cccaagcaag cgtcaaagtc
    20761 gcttttttta attctccaaa gtaacgtaat cctagcacag ccgctgtatt atggagagca
    20821 tcaaacaaaa tgagtccatt tggaaatgtt tcatgtgtcc attcgggatc acaaaagata
    20881 taaagatctc cctcatcaaa ggaaacagac ttttgataag tctctaagat cttcgaattc
    20941 gctggctgaa aattcagcaa ataagaatac aaattgttct caaatccttc tggaatcagc
    21001 aaatgacttt gcacagaaaa ttcttcagat aggccaacga ttacttttgt atggtaaaac
    21061 tggcgcttgc gactttgata cacagcttct cttaaaattt tctcgtattt gctttgttcc
    21121 ttgtctaagt cctcaaagat ccgccgagca cttgctgttc ttcccacaat gcttccatca
    21181 ttgaatacta gtgtcctaac atcagctggt aaatctagtt cttcagcatg tgagattgtt
    21241 aaatctgtga tgatcgttcc tggactccct tttgctagtt gataagctgt cttcctatca
    21301 aaaacttcat gaacattatt accgtaaaat gtcgtttcta caatcgatcg aatactggat
    21361 aacagtggat ttccacttgt gatttcgttt aattcgagac tctcttttgt actcatggct
    21421 cctcctcatt ccctaaacaa aaaaacgttt tctttattat acattattcg agtttttatt
    21481 tgtactgctg cgctgtggga agcaaacgag ttatttttca cacaaaaaaa ccgagaactt
    21541 gaataaacaa gtttctcggt ttcatcattt tccttttatc ggcttagtac cagccgtttg
    21601 ctaaccagaa tgttttagca gcatcccatg aaccataacg agatgttaca tattgttctg
    21661 ctacacgttc ttgattggca gctgaatagt cgccgtttaa gtatgaagca tctaattgat
    21721 aacggccgat gtaacgtccg ttagtagctg tgtatgaacc gcctgattct ttttgtgcga
    21781 tccattcttt tgcagagctt gaagttggcg ctgttgcagc agctggggca gcaggagcta
    21841 cttcaactgt ttcaacaggt gcaacgtatg tatgttcttg cgttgctgtt tcagttgtag
    21901 atgctgtatt ttctgttgtt ttgttttctg ttgttgctga agaatcattt gaagttggga
    21961 ttgttaattg ttcaccaaca taaatgcggt tgatgtttgc gatcgaattt ttttcagcaa
    22021 ttgcatcgat cattgagttg tctcctgcaa atttttgtga gatcttagat aatgaatcac
    22081 ctgattttac tgtataaact tcatcagcgt gagcatttgt agttcctaca aacattgcag
    22141 tagcaccagc agcaaaagtt gtacctaaaa caattttctt aaatgaattc atattaaact
    22201 cctttttttc tccgtttttt attctttcga cgctattaac tttatcattt agaaatattt
    22261 cataggtaaa agaa
//
LOCUS       DASJTC010000047        22165 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.047, whole genome shotgun sequence.
ACCESSION   DASJTC010000047 DASJTC010000000
VERSION     DASJTC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.047"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="U4B19_001573"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="U4B19_001573"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEN2077319.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="U4B19_001574"
     CDS             2370..3848
                     /locus_tag="U4B19_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2077320.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="U4B19_001575"
     CDS             3814..5145
                     /locus_tag="U4B19_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2077321.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="U4B19_001576"
     CDS             5259..7811
                     /locus_tag="U4B19_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEN2077322.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKEAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="U4B19_001577"
     CDS             complement(7818..9257)
                     /locus_tag="U4B19_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2077323.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9603..9965
                     /locus_tag="U4B19_001578"
     CDS             9603..9965
                     /locus_tag="U4B19_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEN2077324.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10152..11009)
                     /locus_tag="U4B19_001579"
     CDS             complement(10152..11009)
                     /locus_tag="U4B19_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2077325.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11129..12040
                     /locus_tag="U4B19_001580"
     CDS             11129..12040
                     /locus_tag="U4B19_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEN2077326.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12047..13698
                     /gene="mdcA"
                     /locus_tag="U4B19_001581"
                     /pseudo
     CDS             12047..13698
                     /gene="mdcA"
                     /locus_tag="U4B19_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13800..14099
                     /locus_tag="U4B19_001582"
     CDS             13800..14099
                     /locus_tag="U4B19_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEN2077327.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14089..15765
                     /gene="mdcD"
                     /locus_tag="U4B19_001583"
     CDS             14089..15765
                     /gene="mdcD"
                     /locus_tag="U4B19_001583"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEN2077328.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15769..16362
                     /locus_tag="U4B19_001584"
     CDS             15769..16362
                     /locus_tag="U4B19_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEN2077329.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16386..17462
                     /locus_tag="U4B19_001585"
     CDS             16386..17462
                     /locus_tag="U4B19_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEN2077330.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17487..18356
                     /gene="mdcB"
                     /locus_tag="U4B19_001586"
     CDS             17487..18356
                     /gene="mdcB"
                     /locus_tag="U4B19_001586"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEN2077331.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18467..19231
                     /locus_tag="U4B19_001587"
     CDS             18467..19231
                     /locus_tag="U4B19_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEN2077332.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19720..21813
                     /locus_tag="U4B19_001588"
     CDS             19720..21813
                     /locus_tag="U4B19_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2077333.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21838..22140
                     /locus_tag="U4B19_001589"
     CDS             21838..22140
                     /locus_tag="U4B19_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2077334.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7180 a         3787 c         4721 g         6477 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagaa gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9421 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9481 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9541 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9601 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9661 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9721 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9781 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
     9841 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
     9901 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
     9961 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10021 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10081 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10141 ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat cattttgatc
    10201 aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa gggcaaatct
    10261 ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac ttttgggaaa
    10321 aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc atctgtcgtc
    10381 gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt atgacggaag
    10441 gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa ttgcagatgt
    10501 tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc tttcatgata
    10561 ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg atcggtaata
    10621 tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt ttccataaaa
    10681 attcggacca agttcggaat agaaacagag gctacctcta aatacaatat gacggaagta
    10741 acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt gtctaatgtc
    10801 ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt tctgccgcta
    10861 tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg gctcaaggca
    10921 ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc gaatactttt
    10981 ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac ctttttgtta
    11041 tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga tagcagtgga
    11101 aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc ctggacaagg
    11161 cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac gtttagaaaa
    11221 tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg tttttatcca
    11281 gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa acggtattca
    11341 gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg cttgtcgatc
    11401 tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa tgaaagaagc
    11461 ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg aagctgagga
    11521 aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg aaaattgtcc
    11581 gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg cagaagccaa
    11641 acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc actgtctctt
    11701 gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc tatctgatgc
    11761 tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag aagagattcg
    11821 taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga tggacttggc
    11881 aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt tgacaaagct
    11941 gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag accaacatgg
    12001 tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg aaaaaccaaa
    12061 ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa tcagcaactt
    12121 gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa aactgattgc
    12181 tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt ttctttcaga
    12241 aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca tgtctagtat
    12301 ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga tcgatttctc
    12361 ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca caatgaaatt
    12421 aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt tgattccaaa
    12481 tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca caggtttcaa
    12541 tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta tcgtgatcgt
    12601 tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg actgggtaga
    12661 cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac gagatcctca
    12721 aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa tctacgcaga
    12781 acacggcgta caatctttga atcacggtat tggattcaat acagcagcta tcgaattgtt
    12841 actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga actgggtatt
    12901 gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa gcatccatag
    12961 ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg tcttttttgt
    13021 cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag gacaatatgc
    13081 catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact cttctaccgt
    13141 aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata atcctggcgg
    13201 tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc cattagggaa
    13261 aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa aaccagtctt
    13321 tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca atgcaatgca
    13381 ccagtgatga tttatagcga agatactaca catatcgtga cagaagaagg aattgcttat
    13441 ttgtacaaag caaaaacaaa agaagaacga caagcagcaa ttgcagcaat tggcggtgtt
    13501 acacctcttg gaatgactag cacgaaagaa tcactagata gcttgcgtgc agccggcatc
    13561 gtgaaattac cagaagatct aggcatcaaa cgtagtgaag cgaaacgttc attattagct
    13621 gcacaaacca tcgatgattt gatcgaatgg tcagatggcc tgtatgaacc accaatgaaa
    13681 ttcagaactt ggtcataaaa ttggcgagaa aaaccttttg tcgccattta cccagatgga
    13741 agaagaaaga cgaataagca gaacgtgtat gctctgtttt acaaaaaggg gagagatcca
    13801 tggaacaatt agcttataaa tatccaacga aacagccgat caataagaaa atccatgtag
    13861 gagtggtcgg ttcaggagat ttggaaatct tgatgtttcc aacagaaaaa acagaatcta
    13921 ctgtaaaaat ttgtacagga agcgatggct tcggcgaagt atggaacaat gtattaacga
    13981 gattttttga tcgttatcca atttcagcag atatcgtgat caatgacttc ggtgctacac
    14041 caggagttgt gtacctacga ttgacgcaag caatggagga actttcagat gaaaaatagt
    14101 tttgtagaat taaacgccag agaacgagtg aacgctctac ttgacaatgg acgtgaattg
    14161 attgatccat tcgatcaaat catcgcgcca agtttaccag cacaaggaat cgtccctgaa
    14221 agtgatgacg ggatcgttgt cagccgtggc acgatcaatg gcaaacaagc agtagtaatc
    14281 gctatggaag gaaaattcca aggtggcggt atcggtgaag tttccggtgc aaaaatcgtt
    14341 gctgcacttg aacatgtgtt agaagaaaat aaaaaaggaa atgagatctt tccagtcatt
    14401 gtactagata caggtggtgt tcgtttgcaa gaagcaaact acggactgtt atctatttca
    14461 gaaatcggaa atgtaatcat tgctctaaaa caatatgtac cagtcatcgg tttgattcca
    14521 ggacgtgtag gatcatttgg cggtatgtcg attacttcag cattaatgag ttatttaatc
    14581 gcaacaaaaa aagcgagagt cggtttgaat ggaccagaag ttattgaaca agaagcaggc
    14641 gttcgagaat ttgattctag tgacaaagac ttgatttgga atacattagg atctcgtcaa
    14701 cgggtagaat caaaaatcat tgatgaatta gtgactgata gtgtagaagc aatcaaagct
    14761 gctgtcatcg cagcaatgga cgaacgaaaa gacacgcatc gcagtgaaag ctcagcattt
    14821 tatttatcat tacttgattc tttagatctt tcaaaaccaa tggccattga cgattacaat
    14881 gaagcaattg ctaaccatca agcaaaatca attgacttac cagaagtagc agaaggaaat
    14941 gaaccagctg cagcaagtgt tggttacgat tggtttgcgg cactaactgg catgaaaaat
    15001 ccaacttctt ctattagtac tgtgtattat ggtaaatcaa gtaaattcat agaagaagcc
    15061 atcgtgacat caatcgtacc tgatcctaaa aacccgatgt accgtgtacg aaacggtgaa
    15121 gttggtttag ttgaaggttt ccgcatggga caaattttgg atcacgttta cgaagaagat
    15181 aaagacaaaa aagtgaaacg gccaatcatc gttgtgatcg atgtaccaag ccaagcatac
    15241 ggctataacg aagaattgat cggtatccat gtggctttgg ctaatagtgc tgctgcttat
    15301 gcaaaactcc gtcaagctgg acatccagtt attggattga ttgcaggaaa tgctatttca
    15361 ggtgcatttt tagcacatgg attacaatca agccgcttga ttgctttaaa caataaagaa
    15421 attaccgtcc aagcgatgtc taaagaaagt gctgcacgtg tgacaaaacg ttcggttgct
    15481 gaaatcgaag cagctgctga aaaagttcca gctatcgctt acgatatcaa taattataca
    15541 aaactaggag cgatctatca tttcctagaa gatatcaccg accaagctgc ttcagaacaa
    15601 aatgtcagca aagtcatcga agcaatcaat gaagccattc aagatgtccg caatacaaat
    15661 gacacgatgc tgacaaatcg atacacaaac gaaacagcca aaacattcgg acgtgtcgaa
    15721 acaaataaag tccgtgccaa aatggacgaa gaatgggatg cataaaaaat gattcaagca
    15781 catgatattg tcctttttga taaacatgaa atagaagggt tcaccttgcc tgaatgggtc
    15841 gttctaactg aaaagatgtt cggaacagtt cgtcgtgcgt cgacggatca cgcagaaata
    15901 ctaccgattg gtttacgtgg acctgcccgc aatcagagat ggtcaacaga aatctcaaaa
    15961 catgccgtca aacaagtcat tcatccgtgg gacatcatcg aacaagaatc gtatagacaa
    16021 gagaaaatca tggattatcc tgtttatcag caatttcatg ctgctagaaa catattatca
    16081 ggatgtaaat ggggaatcgg cggcagctta ggatttgaat tatcgactgg gattccggca
    16141 gtaaaagaaa ccagtgattt tgatttgctg ctgtacgcaa attcaccgat tgaattgcca
    16201 attcaggtaa ttcaatcgca tcctgctttt tttgagcaat tcgataccca agtagtgact
    16261 cggcaaggcg gtttttcact aaaagagtat ttgagagagc cagagaaaaa aattctattg
    16321 aaaacagtcg aaggacctaa attaaccaaa gaactttggt aataaagaaa aagagagaga
    16381 aagagatgtt tgaaaacaaa aaaatcaaaa tcaacgtaag tgacttccag aaagtatcag
    16441 ctgtaacggc tgttatgatg caaacgatat tgagctttgc tttggcaaac agccacagcg
    16501 caacaaatac agcttttctt ggtacctttt acgtcatcgt taaatacaca gcaccaatgt
    16561 ttatttttgc aattgtttat aatatggtaa aaacaagtca gcatttgtct tattgggaat
    16621 ttttgaaaga aaagtttttt gaactagtcg ttccgtatct tctatggacg acggcttatc
    16681 tttggctatt tccagcagtc caacaacaaa ctccgtatac ggatactgca agctttttac
    16741 tgaaatatgt cacaggtgat ggagcgcctc atttatggta taccgttatg atgctgcaga
    16801 tccaattgtt catgccgttt tttgtttggc tcggttacaa agtatttgct aataaaaaat
    16861 atgtatggcc agtattgatt ggttcaacga ttttatatgt cgcatggtat ctgttctatc
    16921 aaacacaagt attgactggg ccttatcatg acagctggta cttattagat cggtttgttg
    16981 ctagtttcat gatttatggg gtagccgcat ttgtttatca cgaaaaagtc tatcaatatt
    17041 tggacagagt acgttatctt ttcttacctg ttgccttagt tatcgcgttc ttttctgtcc
    17101 gttcattgct cgcacatcca ggagacttga gctttgcgaa tgcaccttac ttgaatacga
    17161 tccaaagttt atacagttta gtcatcatct ttgctgtctt cataggcgct tccaaaatga
    17221 ttgtaaacga ttcgccaaaa ttgccattgt tcaaatggtt gtctgtttat gcttatcgta
    17281 cgtatttagc caatgtattt gttttccaag tactgttatt attgtttaaa gatagttggc
    17341 tgcagttacc ttcaggagtc atgattcttg tagcttatct aatgacggca agttgtgcat
    17401 ttgctttaag ctggttactt catatcattt gggtagctat caaaaaaggt ttcagcaagt
    17461 aatagtagga agaaggagtg agtgtcatga taaatgacga acgaaaaaaa gcagtgactc
    17521 agcaactcgc tgcgttggta gaggaagcat tgatcgcaga agtaacatta agtccaaaac
    17581 ctggattagt ggatgcactg gatgcgggtg cacattccga tatggactat gctttatttc
    17641 ttaagtctgc taagacgcta actccttttt ttgaagaaat ggctcaaata gcatggcatc
    17701 atgctattga tcaggagtta agagaaagaa ttgctgagat tgggcgaaaa gcggaaaagg
    17761 caatgctgat tgctacaaat ggcatcaata cccataaagg agcgatttgg gcaatggggc
    17821 tattgatcag tgtcattgca caaaaaatca gtatggaaca gcctttttct ttatcgaaaa
    17881 tacttgatga tgtcagcaag cttgctgctt ttcctgatac aaaatatcgt cataaaaaaa
    17941 ccactcatgg tcatagggtg aagcaaaaat atggcgtaaa tggtgcatat gaagaagcga
    18001 tattagggta tccgcatatc aggcttgcaa ttcaagcagc taaggagcta gataatgatt
    18061 catatgtaca gaaacaaatg catatgttgt tagttttgat gagttcgcta gatgacacat
    18121 gcgtattgca tcgcagtgat cagaaaacgt tagtggaaat gcaaaaactt gctcatgctg
    18181 ctagtcaaag tgctttacca aatcgtccat ttacagcact gttgcacttt tgtcaggaaa
    18241 accaaatctc tcctggagga agcgcagatc tcttgtctgc aagcttgttc atgctgaaga
    18301 tagaggaatt atggactgcc gacacataca tttgtccgat gttactcaat gactaaataa
    18361 gacaaaattg ctaaaaaaac agtttatgca tctggttatt gactcttcct tttagcaagg
    18421 ctatcataaa aatagttttt tgtaataaaa gataacgagg aagacaatga aaaaaatcaa
    18481 ttatttagca atcattttgt tagtgggcct atgtttgttt gctctgccga cccaaggatt
    18541 cgctgtcgcg ggtggtcatg gtggaggtac atctagcggt cgaagttcgg gcagaggttc
    18601 atttggtggt cgttcatatg ggggcagtta ttttggcagt cgttcttacg gcagagattc
    18661 ttatgggtat tatggtccag gtacaggtcc ttctagttat gagcaaacgg gatttcttgc
    18721 attagctgtt ttgtcagtag gaggggtttc tgctttttct atcgtcaaga ataaaagaca
    18781 aaagaaaagg ctgaaagcgg agatattgtc ccgcatgtca ggaacggcta aagaaaagaa
    18841 agcacagttt ctggaaatcc aaaagatatt catgaagatc caatccgctt gggaagaaga
    18901 aactcctgaa aaagccagaa gctgttatac agacaagctg tttgccgaac atcagaaagt
    18961 tctgcaaaaa aacaaagcag acggtgtaag aaatcatact aaaaaaataa ctattcagga
    19021 agtaggaaat tatcgacaga tccacgaaaa tagttttgcc atccgaattg atttcagatg
    19081 ctgtgattat tcagtcgatc gaaaagatgg gcgattgatc tcaggaagta agacacaacg
    19141 acagtatttt tctcagatat ggtattttga tttttctgag aaagaaaaaa gatggcaagc
    19201 cgattttatt cagccgatta tgctggattg acaggaaata tttatgaacg gagttgtgac
    19261 aaaagccttg tcgcagctcc tttttctgaa taaacggtgt gccagagata gcctatcggc
    19321 aataaggcac gattttgtaa aatatgggaa gcgatttttc aaaatcgctt cttatcgctc
    19381 aaggctgaac atctctgtca caacctctcc actaaatggt gtttaaaagc ttgcatctgc
    19441 gataataaga acaaccaaac gaaaaatagg aagcactatt ttagtttagt tgttcttatt
    19501 acttgatgca ggacagcttt ttcacaacct cttttttgta tagatgggca aaatttttgt
    19561 aataataggc gtatgtcttt ttcgttgagg agatgaaaaa gcatatggct tattttttgt
    19621 ttgctttgct tactttcttt ccacttcaag tggcagaaat atctactttt tattatgtaa
    19681 gcggaaccat atacttgaaa tcagaaggag atcatttcta tgaaaacaag aacaattgaa
    19741 atacttcaca gaatcatgac agcccaatat ccagtaaaag caaaacatct agcaagtgag
    19801 ttcaatgtca gtcagcggac gatccgtcag gaagttcttg aagcgaatat ctggttgagc
    19861 agtaaaaagc ttccggagat ccgtacaatt cgcaacaaag gttttttgtt gaagctggaa
    19921 aaggaaaagc aagctcgatt agaaactgaa ataaaagatg tgaaagacgg ctttttgaat
    19981 cggcgcgaac gtatttttga tctagtttta gccgttgctt acagccgaat cccgatccat
    20041 ctaaacaaaa aagaagaaga atttcaaatc tctaaaagta cattagatga agatatgaga
    20101 cggttacgag caacactggt cagttatggg atagagatcg tcagttatgg aaagcaagga
    20161 ctggtttata aaggttcaga acgatctatc cgtacgatga tctatgattt tatcaatcag
    20221 aatctgggaa tgaccgactt ctttatgaat caagatgcac aattaacacc ggctcaaaaa
    20281 atctttctcc attattttcc tttggtggaa atcaaaaaaa tacaggagat ctattccaat
    20341 aagttgttga aacaagaaga tgatatttat aaaaaacaaa tcctgctttt tacattgatc
    20401 tggatccagc gaatcaggaa acatgaattt atctctgcca ttaattggaa aaatacagaa
    20461 ataaaagaca gtagcttttc tgcatttatt gaccacatga tcgaggaatt tcaattggac
    20521 aatgtcccgc aagtagaaag aaattatctt cgtttcacta ttgagacgtt caatgctaga
    20581 gatatctcca attcaatcga gtgggtacaa gctcaattac taacgatcca attgatccaa
    20641 tttgtagaga acgagacaca cgtacctttc catctaaagg aagaagcttt attcgaaaat
    20701 ctctataagc acatggctgc actgattgta agaatcaaga atcatatcca agtcatgaat
    20761 ccattgaaag aaaacatccg aacaaattat ttcccaatct accgcgcagt cactcatttt
    20821 gttcctacga tcgaagaagt aattggtggc aagatactgg aggacgagat agcttttctt
    20881 gtcatccact tttcgacgat cgccagtgta atcaagcgag acttgaatta tgtttataaa
    20941 tcagtcgttg tctgtaacca tgggatggct actggaaatc tgttagcaga gaatctgaaa
    21001 gaaaagtttc ctcagatcga gattactgca gttttaagtt caaaagaaat cgaattagta
    21061 gataaattgg atgtcaatct ggttttcagc acctttcaat tgaattatcg aaataagccg
    21121 cttttagttg tcgatccgat tttaactgag acgaatatct tggttataga agactttttg
    21181 aaaagaaatg aagaactaca gcggttagag cctaacagtc atgaatcgac ggagttattc
    21241 actcaaataa tagatgtcat gaaagaaagt ggtggccgag tagatcgaat catctatggc
    21301 aagttagaaa aattattcga aacaaacaat ttagaaatca acaaaaggga gatccagcct
    21361 atgttaaaag atattttgac ggacagcaat atcatgatca aagaaaaagc cactacttgg
    21421 gaggaatcga ttgaactagc tgctagacca ttggttaaag aaaacattgt cgaagagcgc
    21481 tatgtcgaag cgatgattcg tgcagtgaat gattacggac cgtatatcgt gattggcaaa
    21541 cacatggcac tagcccatgc ccgaccagaa gatggggtca ataaattagg agtcagcgta
    21601 gctacgattg agcaaccaat cgatttcggt aatcctgaaa tggatccagt aaagattatt
    21661 ttttgtttgg cagcagtaga ctcctactcg catctgaaca tcatgaaaga actaattgaa
    21721 ttgatcaaca acgaagagaa gttggatcga ttgattgaat gcacaaatat cgaagatttc
    21781 aaacaactgc ttttttcagt agttgagaaa taataaatga tacgggggag aacaaagatg
    21841 aacaaattga acattttatt cgtatgcggt gcaggtttag gaagtagttt tgcagcacag
    21901 atgtctgctg aggatgtttt gaacaaatta ggagtagaag ccaaactgga ccattgtgat
    21961 atttcttccg cagtttccat gaaaccagat gtgatcatca cagcagaaaa ttttcgttcg
    22021 cagttcgaaa agttcgcagt tgatcctaaa acggcgatga tctatttgaa aaatattgtt
    22081 tcaaaaaatg aaattgaaga aaaaatcacc ccagttctgc aagataaggg agttatctaa
    22141 atacaaaaag aaaagagggt aaaaa
//
LOCUS       DASJTC010000048        21880 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.048, whole genome shotgun sequence.
ACCESSION   DASJTC010000048 DASJTC010000000
VERSION     DASJTC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.048"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            75..629
                     /locus_tag="U4B19_001590"
     CDS             75..629
                     /locus_tag="U4B19_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2077335.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(802..1275)
                     /locus_tag="U4B19_001591"
     CDS             complement(802..1275)
                     /locus_tag="U4B19_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2077336.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKIKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1287..1823
                     /locus_tag="U4B19_001592"
     CDS             1287..1823
                     /locus_tag="U4B19_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2077337.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1863..2234
                     /locus_tag="U4B19_001593"
     CDS             1863..2234
                     /locus_tag="U4B19_001593"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2077338.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2518..3789
                     /locus_tag="U4B19_001594"
     CDS             2518..3789
                     /locus_tag="U4B19_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEN2077339.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4034..5059
                     /locus_tag="U4B19_001595"
     CDS             4034..5059
                     /locus_tag="U4B19_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2077340.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5353..6462
                     /locus_tag="U4B19_001596"
     CDS             5353..6462
                     /locus_tag="U4B19_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN2077341.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6545..7441
                     /locus_tag="U4B19_001597"
                     /pseudo
     CDS             6545..7441
                     /locus_tag="U4B19_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7457..8281
                     /locus_tag="U4B19_001598"
     CDS             7457..8281
                     /locus_tag="U4B19_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2077342.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8313..9623
                     /locus_tag="U4B19_001599"
     CDS             8313..9623
                     /locus_tag="U4B19_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEN2077343.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9771..11030
                     /locus_tag="U4B19_001600"
     CDS             9771..11030
                     /locus_tag="U4B19_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEN2077344.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11040..11912
                     /locus_tag="U4B19_001601"
     CDS             11040..11912
                     /locus_tag="U4B19_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2077345.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11924..12754
                     /locus_tag="U4B19_001602"
     CDS             11924..12754
                     /locus_tag="U4B19_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2077346.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12794..14977
                     /locus_tag="U4B19_001603"
     CDS             12794..14977
                     /locus_tag="U4B19_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN2077347.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15038..16258
                     /locus_tag="U4B19_001604"
                     /pseudo
     CDS             15038..16258
                     /locus_tag="U4B19_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
     gene            16255..17715
                     /gene="gtfA"
                     /locus_tag="U4B19_001605"
     CDS             16255..17715
                     /gene="gtfA"
                     /locus_tag="U4B19_001605"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEN2077348.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17792..18898
                     /locus_tag="U4B19_001606"
                     /pseudo
     CDS             17792..18898
                     /locus_tag="U4B19_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19220..19429
                     /locus_tag="U4B19_001607"
     CDS             19220..19429
                     /locus_tag="U4B19_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN2077349.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19422..19571
                     /locus_tag="U4B19_001608"
     CDS             19422..19571
                     /locus_tag="U4B19_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEN2077350.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19725..20390
                     /locus_tag="U4B19_001609"
     CDS             19725..20390
                     /locus_tag="U4B19_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2077351.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20674..21759)
                     /gene="xerS"
                     /locus_tag="U4B19_001610"
     CDS             complement(20674..21759)
                     /gene="xerS"
                     /locus_tag="U4B19_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEN2077352.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
BASE COUNT         7436 a         3294 c         4211 g         6939 t
ORIGIN      
        1 tatataagtt aatgattatg gtaaaatata tacataatca ttaactcaga atatttttaa
       61 gaagggaaac aatagtgagc tataatgtac aaccgttaag gacaaatgaa gagatacaag
      121 attttcagtt ttggctaaga agaacaagta atccggaaag agatagtttt ttatttttgt
      181 ttggaatcaa taatgggtta cgtatgtcag atattatagg attaaaagtt ggagatatac
      241 gaggcaaaag taagcctatt attgttgaac ataaaacagg taagcgaaaa ccgattttta
      301 tagataattt gagagaagaa attttattat atactgaagg taaagaagaa aatgattggt
      361 tatttccgag tcggcaacaa ggtcgacata taacaaggga tcgtgtctat cagatatatg
      421 cagatatagc agaaaaatta ggacgagatg atataggaac acatacatta agaaaaactt
      481 ttgggtatca ctactacaag aaaactagag acattgctac cttaatgttt atttttaatc
      541 atagctcgca agcaattaca aaaaggtaca ttggtataac tgaagatgaa ataggagcat
      601 cacttcgtgg atttaaacta ggtgtataat ttaaaaattt ttataaacag taaagacaaa
      661 gattaaaagc tatttttatc tagaacgatt ttatttcata aagttcgcgg aataaaagct
      721 ggggttactg gtacttggta taggttcgtg ttatataaac taagtaatta aataacattt
      781 ttaggttgga aaaggatgaa attaaaaaaa gagaatcaat acggtctcaa aaacgcaaac
      841 gctgcggaat aagaaaaata ctgataactc aacgtttagc agactatcta atagggatag
      901 tctgcttttt tcgtttttga ccatactttt gatcttctgc aaacttcctt gcttttcctc
      961 tgtagaactc atgaaatctg caaaacgaat tgccgtttcc tctgtctgct ttttcgcgat
     1021 atgggtgtat atatcatcca tagtggtttt aatatctgta tgtccaagtc gaacatacac
     1081 ttctttgaca gacgcaccag attcaaagag cagactacaa tgggtatgac gaaaaccatg
     1141 aagtgtgata tgttttaagt tattctcttt aatgatgatt ttcaaattgt gatttacata
     1201 gtctaaataa aggggcttat tttcttctga tgaaaacaaa agttgttcac catcttttat
     1261 aggtttgatt ctcattttta gatattatgc ccgcacgtat tcatcacatt tctattgtga
     1321 atcgctttat tcgaccgaca tttgactttt atcataatat actcggttta aaattactaa
     1381 tgaaaacaat taatcaagac gatcatacca tgtatcacct gtttttttca gataatcacc
     1441 accgtgtggg aactgaactg actttttttg aagtacaaga agggaacaac cagttttttg
     1501 gaaccaatac gattgaacga acgatactaa aagttccttc cgaagcatca ttgcattttt
     1561 gggaaatata ttttgaaaca caaggcgttt gccattatgg aatcgaatca ttttctggac
     1621 gacctatctt acgttttgaa gggccagatg caacacgatt agctttagtt cccttacgcg
     1681 aatttgaaga tattgatgat tatttcccgt atgtcacaga ccagatccct acagagcatg
     1741 ccattttagg aattgatgcc attcaactac gcgtacaata ttccgatgcg actgaacgag
     1801 agttattgtc ggtaggctgg tagcacaaag aatacgtcag tttcttttcg ggtcatcaac
     1861 gagtgacaat cttagaaaat caagacactc agttctatca agaagttcag attattcaag
     1921 atcgtgagaa tccagttgcc attgaaggga ttggtagcgt ccatcatgtt gcgtttggcg
     1981 tagaaaataa atctgattta caacgcatcg acaaacaatt acaagagcgc aattttataa
     2041 attcaggaat aaaagatcgt gaattttttg tatcacttta ttatcgtgac ccaaatcaat
     2101 tattgattga aattgctaca ggggaaggaa acctagatgc aaaagcgtat gaaaatcaaa
     2161 gtccacgctt tgaagaaatt cctttatttt taccgcagta cttaaatttc attcgtgaac
     2221 aagtggagca ataattagct aatcaaatcc atgacgaaga gtaagaggtt gactcataag
     2281 tcaaaaaata aagaaaaacg catcaaatcc attctaaaga atgttgattt gatgcggttc
     2341 ctatttttat aaactatcat tttcgactaa taaaactact tatgagtcag cctctctttt
     2401 tttcttgatt tttatacctc tttctaggta aactaaacat ctgccaaaaa taccgtgtct
     2461 cattgaaaca ttctcaacta tttcaaaata aaggggagta ccaaatgaac gagtatattg
     2521 aaattatcca cgcaagagaa acaacttaaa aatgtcagtc tcaaaattcc caaaaataaa
     2581 attagtattt ttaccagcgt atctggttca ggaaaatcat cgattgtttt tgatactatt
     2641 acccaagaag ctggtcgtca actgaatgac acctatgata gttttacccg tttattttta
     2701 cctaaataca gtcgtccaga ggttgatgag attgataatc tttctacggc gattgtggtc
     2761 aatcaaaaga agattggtgg cagtgcgcgc tcaacgttag ggacgattac taacatcaat
     2821 gcgttatttc gtgtgttgtt ttcatgtttt gcaacaccta gtttaggata tgcaaatgct
     2881 tattcattta atgatcctag tggaatgtgt tctgtctgtc agggaatcgg taagacgatt
     2941 gcatttttaa taggattgct ttatttttcg gctattgata atgctgattt tgtattttct
     3001 gatccattta ccgcagaagt tttgtctgga attgaggaac aggcaaagaa tcatggttat
     3061 tttgtattgg tgcataatgt taattcagca gaagatgtta aaactattca gagaaattgg
     3121 cgttttgagg ggttcattgt tgttggtgta cgtgtaaaag aatttttgga attaaataac
     3181 gtttttgatg gtcctgttgt ttatattgat acgcatcttt caaaagagac attaagagaa
     3241 cttgaacaac aaatgaatcg tttatttgta aatacaaatg attaccaagc tagtacattg
     3301 gcagtggaac atttgattca ttatggtcat gagaaaatag cgtttctttc ttttgactac
     3361 attgtaggtg aaccaagtgt tattcaagaa cgacatcaag cttatatcga tacattaaag
     3421 aaatatcatt taacctttaa atcgcattgg atatataaca actctgaatt tgagcgaatt
     3481 tatcaagaat tagctgaatt tactgcaatt gttgttactg gagattattt agcggctaaa
     3541 ttagtaaaat ttttaaaaaa taagaataaa cgagtgatgg aacaattatc gattattgga
     3601 tttgatgata ttacttttgc agaattaatg gatcctgcgt tgacaacaat acgactaaat
     3661 ccaggtaaca agggggcgat tgcttttcac caactgattg gactggccaa acaagaagaa
     3721 ataaaaaatc aagtactact attagaggga gaattgattt ctcgagactc tgttcacaaa
     3781 atcaaataaa aaagctctgt gaaataagta tagttaacaa taaaatatcc gaacgataga
     3841 gagaatgaac tgtgatctag cagttcctcg tcgcaaattc actagaatta gcattattta
     3901 ctctaaattg ttcggatatt ttttcttgtt tttaattatg attacagtgc ctatttttaa
     3961 cgaaatttgc ttattttcaa aatgtatgat tatatgttag taaacacttt caaatatgaa
     4021 aagaggagct attatggtag gaattaggga tgttgcaaaa cgggcgggtg tgtccattgc
     4081 aacagtatcg cgcgtgctaa atgaggatcc gacactttca gtaaccgatg aaaccaaacg
     4141 aaagattgca gaatcggtta atttttatca ttatgtcaaa aaaagtcccg ttgcaaaaaa
     4201 tttaacaaat attgcattaa taacgacagt ttctgaagta gatgagttgg aagatcccta
     4261 ttttcgaacg attcgcagag ggattcaagt cgaagcagaa caaagcaaag tcaatttgaa
     4321 gaaaattatt cgtttgtcag aaacaccgtt ggagcaagac gatttgctaa aatgtcaagg
     4381 tgtgttgatt attgggcagg tgctatcttc tgtgattgaa gaagtatcag caattaattc
     4441 gaatgtcgta gtgattgatg accctagtat tgaagctgaa gtagatgcag tttatactga
     4501 tttaacaaaa gcaacgaatc agcatttaga tcgattgtat aaaaaaggac accgaaacat
     4561 tgcgtttatc ggaggaggac gtgttcgttt agatagccaa ggaaataaac acgtgtcaga
     4621 tgatgattat cgaaaaatag cctatgagac gtggatgaaa caaaaaggat tagaaaaata
     4681 tagtcaaatt tatttagaag gctggaccac acttgacgga atgcaggcag cagatttact
     4741 tttgaaaaaa catgctaaag ataagttgcc gactgctatc atggtaggaa atgatcctat
     4801 tgctgtgggg gtttatcgtt cacttcaaaa aaatggtcta catattccag aagatatttc
     4861 gattgtgagt tttgataata ttgaagttgc tgaattttta acacctgcat taaatacggt
     4921 gaatgtgaat acagaagaaa ttggaaaact agcagttcgc atggttaatg aacgaataaa
     4981 agaagttaga aatgtttcga tcaaagtgat tgtttctaat aatattattg ttagagaaag
     5041 tgaaaaaaat atcgattaaa aaagtaaaca tccaaaaaag ggtgtttact tttttgttta
     5101 ctaatatttt tagtaaaaaa tctcgacaaa acattaagta aacagttatg gtgttgttaa
     5161 caaaaagaaa acgcattcaa aacaggagga attaagatga aacatttcaa aaaaatagct
     5221 ttaacaggga tagctgtttt aactgggaca ttactcgcag catgtggtgg gggagatagt
     5281 aattctaaag acgggcaagt aactttaagc tatgcgagtt gagatactga ccaagctcca
     5341 ggactaagaa aaatgttaga tgaatttgaa aaacaaaatc ctgatatcaa agtagaaatg
     5401 gagacaacgc cttgggatca atattgggtt aaattagaag ctgcaacaac gggaggtaat
     5461 atgccagatg tagtaaccat gcactctagc gaatcctata aatatatgtc acagaatgct
     5521 ttgatgggac ttgatgatgt tatcaaagaa aatgatattg acatgggaaa ctttactgaa
     5581 ggaattgctg atttctatac gcttgaagac gaactatacg caatccctaa agatgcttct
     5641 gtagtggggc tatggtacaa caaagaatta tttgataatg caggtgtcga ttatccagat
     5701 gaaacatgga catgggaaac actcagacaa gctgcaattg atttaacaga tgaatcaaaa
     5761 ggcatctatg gttttgcatc acatattgga acagagactg gctactggcc atttatttat
     5821 caaaatgggg gagaagtatt tgccgatgac aatacaaaat caggtttgaa taccccagaa
     5881 gcacaagaag ctttagcttt ttatcgagat attgcacttg ttgatcatgc ttcaccaaca
     5941 attacagaat tacaagatcc tgataagtta agtcgttttc aatcaggtag attagcaatg
     6001 atgattgaag gaaactggtt tgcaccggga tttttgaaaa atgactatct agttgaaaat
     6061 gttgcttctg ccgtgttacc taagggaaaa gttaatgcaa caattacaaa tggacttggt
     6121 tgggctgcca gtgcgagtac aaaataccca gaagaagtga aaaaactaat ggcattttta
     6181 gctagtgagg acgccaataa aatccaagcg gaaacggggg catcaatccc agcactaaaa
     6241 ggctttgaag acgagtgggc tgcttcttca gatacaattg atttgagtgc ttttggaaaa
     6301 atgttggagt atggagtccc tcgtccatac aataaagatg gattacgagc tgaacaagca
     6361 gaagttcaaa ttattaaccg tattctgtct ggtgaagtaa ctgttgaaga tggaaccgct
     6421 caaattgaaa aagaagtcaa tgcgatattg agcggaaatt aaattagtta attatacaat
     6481 atacaggcga aaggattcta tttcgtctgt atattaatgg atgaatacca aaaaggggga
     6541 tattatgaaa aatcgaattt ttggaaaata tcgtaaagat caagtgatat ggggctatat
     6601 ttttattttt ccaacggcat taggattaat tttattaaat atatggccag caattcaaac
     6661 agcaatttta agttttcaaa aaacggtagg gttcggtgat actgaatggg tcggattaag
     6721 aaattaccaa aaaatgtttg cagacggtga agtttttcag tctttattaa acacattaat
     6781 ttatgcagtg gtttcgatac ctagtattgt tgtattatcc ttgttggtgg cagttttaat
     6841 gaatagaaaa gttaaaggat tgtcgattta tcggacaatt tattttctac cagtcgttgc
     6901 tgcaccatcc gcagtcgcaa tgatttggcg ctggatgttc aataatgatt atggactgat
     6961 taataacatg ttggctaaaa taggtctcga tggaccagca tggttaacgg atccatcgat
     7021 tgcgatttat tctattatca ttgtaggagt ttggagtgct attggttata acatggtgtt
     7081 attactagcc ggcttataag aaattcctaa agattattat gaagcagcgg aaattgatgg
     7141 tgctagtcca atccagcagt ttttcaacat tacgttacca ttagttacac caacattatt
     7201 ttttgtagtt gtaacaacag ttatcaatgc tttccaagta tttgatgtta tttttatgat
     7261 gattactcct aatagtaccg ccatgtttaa atcacaatca ttagcctatt tattctataa
     7321 acattcattt attttaaatg acaagggata tggttcggca atcgttatgt tccttttagt
     7381 gattatatta attattactg caattcaaat gaagttgcag aaaaagtggg taaactatta
     7441 aggaggctat caaattatga aaaataaatt tttttcaaat gcgccaatcc atatcatgct
     7501 tattttaggt ggtattgcaa tggcattacc ttttatttgg atgattttaa cttcagggaa
     7561 aacgttggtt gaatctaccc aaattccacc tacaattttc cctgagaaat ttcagctaag
     7621 taactatcaa caagtacttg atttattacc attcatggct ttttatatca atacattttt
     7681 aatggttgtt ggacgagtga ttggctcggt tttattttca gcaatggctg catatgcttg
     7741 tgcgcgtcta agttttccag ggaaaaatat ccttttcagc ttagtactta ttcaaatgat
     7801 gattcccggt tccattttta ttactccaca gtatttatta gcacaaaaat tgggtatttt
     7861 aaatacaatt ccagcattaa tattaccagg ggtagttagt gcgtttggta cgtttttatt
     7921 aagacaattt ttcttaggtt tacctgatga attggaggaa gcggcctatt tagaaggtgc
     7981 caatatttgg acgatctttt tcaaaattat gttgccgctt gctaaatcag gattggttgc
     8041 acttggaata ttcacagcgt tgtttgcatt taaagattta atgtggccat tgattgtcaa
     8101 tacttcaatt gataagatgc ctttagctcc gggattagca aacttacaag gacaatatgc
     8161 cacgaacttc ccacaattaa tggcaggttc gttattagca atttggccaa tagttgtgat
     8221 attcatcgtt ttccaaagaa aattcattga aggaattgct acaacaggta gtaaattata
     8281 aaactattaa ttagtgattg gagagtaagt acatgacaaa aattacattt attggtgcag
     8341 gaagtactat ttttgcaaaa aacgtattag gcgatgcgat gttaactcca agtctacaag
     8401 atgcacatat tgcattgtac gatattgatg aaaaacgact aaaagaatca aatttaatgc
     8461 ttaaaaatat taataaaaat tcgaatgaaa atcgtgctga tattgtttct tattatgatc
     8521 gcaaagaagc gttaagagag gctgattttg ttatcaatgc tattcaagtg ggtggttata
     8581 aaccttcaac agtgattgat tttgatattc ctaaaaaata tgggttgcga caaactattg
     8641 gtgacaccat ggggattggt gggattttta gaaccctacg gacactacca gtaatgttag
     8701 actttgctaa agaaatggaa gaagtttgtc caaatgcttg gttattgaac tatacaaatc
     8761 cgatggcgat gttaaccatg ggtgttttaa aagcgacgag tattaagact gttggtttat
     8821 gtcatagcgt tcaagtatgc gtaccagaat tgtttgagca tttaggaata gccgatcaat
     8881 atgacttaaa tgactttcaa tggaaaattg caggcattaa tcatatggca ttcttgttgg
     8941 aaattacaaa aaatggggaa gatttctatc caacaattcg tcagttagct gcagaaaaac
     9001 caaatccaca tccagattct gtacgttttg aattgttgaa acgttttggt tactatatta
     9061 cagaatcaag tgaacacaat gcagaatacc atccatactt tattaaatca aaatacccag
     9121 aattgattga cgaattaggg attccgttgg atgaatactt acgtcgttgt gaacaacaaa
     9181 ttaatgattg ggaaagcatg cgagaggaca ttgtaaataa tggggagtta acacatacga
     9241 gaagccgtga atatgcttca tatatcatgg attctattac gacaggaact ccgtcagtga
     9301 ttgcaggaaa tgtcttaaat aaaggcttaa tcacaaactt accgacagat gtttgtgtgg
     9361 aagtaccttg tttagtcgat aaaaatggcg tgcaaccaac ctatgtcggt gaattgccaa
     9421 cacaattagc tgctttaaat cggacgaata tcaatgttca agaactgaca gtagaagccg
     9481 ctttaacact agataaatca aaaatttatc aagcagcgta tttagatcca catctatcat
     9541 ctgaattgtc tttaacagat atcaaaaatt tagtggatga tttaatcgaa gcacatgggg
     9601 actatttacc tgaatataat taaaaagaag ctgaaagagt aaggaatttt caattcctta
     9661 ctcttttctt agttaaattt agtaaaaata ttgacaaaac acttagtaaa cagttacatt
     9721 agtgttaaca aaaagaaaac gcattcaaat tgtataagga ggagcataaa atgaagtggt
     9781 ctaaaaaaat tattttagct tgtatagcac taggagcaac gatctctttg actgcttgtg
     9841 gaaaaaatca ggaagatgca agcggtaagg tagaaataga gttttttaat caaaaaaaag
     9901 aaatgactca aacgattcag gagattgcta aagattttga agcaaaaaac cctgatattc
     9961 atgtaaaagt tgtggatgta ccgaatgctg gtgaagtaat taaaacacgt atgcttgcag
    10021 gcgatgtacc agatgtcatt aatctttatc ctcaaagtat agaattgaaa gaatgggcga
    10081 aagctggtta tttagaggac ttaaccgaag aaccgtattt agagaatatc aaaaatggat
    10141 atgcacaacg ttttgctatt gaggatcgtg tatatagcat accattaacc gctaatgtct
    10201 atggttttta ctataacaaa actgcttttg aggaaatggg gattcaagca ccagaaactt
    10261 gggcagaatt tgaaaaaata gtagctgaga ttaaagatca aaacaaagta ccgtttgcga
    10321 ttgcaggagc agaaggctgg actttaaatg gttatcacca actagctata gcgactgctg
    10381 ctggtggtga agaggaagcg aacaatgttt ggcgtttctc agaagttaat ggaattaatg
    10441 ctgaaagcaa agaaatgcaa aaagatttta atcgattaga tttgttaaga gaaccaaaag
    10501 ctttgcaaaa caactggcaa ggtgccggtt ataacgatac agtagttact tttacaaaag
    10561 gtgatgcgtt gattatgccg aatggttcat gggcaatgcc gatgattcat tcacaaaatc
    10621 cagattttga agtaggaact tttccttttc cagcagatga agcaggtcaa agtctaacga
    10681 ttggcgcagg agacctagcc ttatcagttt ctgcaacttc tcaacataaa gaagaagcca
    10741 aacgttttgt agaatatatg acaacacctg aagcgatgca gaaatattat gatgtagatg
    10801 gtgcaccgtg tgctgttgag ggcgttatcg agaatactca ggattcacca ttaagtggtt
    10861 taactgaact tgcttttact gatcgtcatt tagtttggtt agccaaagat tggaatagtg
    10921 aaaatgattt ttatacctta acaacaaatt atttacacaa tggcaatcaa aaagccatga
    10981 ttaacgcatt aaatgctttc tttaatccaa tgaaagctga cggaaggtag gaaaataaaa
    11041 tgaaaataaa agaaatttta aatcgttact gggcaatcat ttttgtagca attccaatta
    11101 ttttgcagat gatttttttc tactttccaa tggttcaagg tgctttttac agtttaacta
    11161 attggactgg tctgacttat aattttgatt ttgtgggttt gaacaattat aagattcttt
    11221 tttctgatcc gaagtttatc aaatcgattg gcttcacttt aattgtgacg ctttgcatga
    11281 ttgctggaca aattggtttt ggtattttga ttgcgcgggc gttgaacgca aatattaaag
    11341 ggaaaacatt ctttagagca tggttctttt ttccagcggt cttatcgggc gtggttgttt
    11401 ctttagtatt taaacaaatt tttaactatg gtttaccaaa aattggtagt atgttaggca
    11461 ttgaattttt acaaaccagt cttttaggaa caacatgggg agcgattttt gcagtaattt
    11521 ttgttttact ttggcaagga attgccatgc cagtcatcat tttcttagcg ggttttcaaa
    11581 gtattccaca agaaattatt gaagctgcta gaatggatgg ggcgaatgat aagcaaattt
    11641 tttggaatat tgaaatcacc tacttattac caagtatttc aatggtattt attatggcat
    11701 taaaaggtgg cttaacagca tttgatcaaa tttttgcttt aactggtggt ggaccaagcg
    11761 atgccacaac atctttgggg ttacttgttt ataattatgc ttttaaaaac aatcagtttg
    11821 gttatgcgaa tgctattgct ttagtcttat ttatcttaat tgggattgtt tcactggcac
    11881 aattaaaatt atcgaaaaaa tttgaaatct aggaggtttc ccaatgaaaa catcaaaaga
    11941 aaaaaatatt tctaaatact tgcttttatt attagggggc ttcatgattt tagttccctt
    12001 agtttcaacg ctattcggtt catttaaaac aacacccgat attatgaata atttctttcg
    12061 ttttcctaat ccagcgactt tggaaaattt ccgaagattg ttagcggatg gaattggtca
    12121 ttacttttgg aattcggcaa tcatcacaat cattgcagta gttgctgtga cagcaattat
    12181 tcctatggct gcttattcac tagcaagaaa aatgactcgt gggaaagcat acggattgat
    12241 gtatttattt ttaattttag gaatttttgt tcctttccaa gtaattatga ttccgattac
    12301 cgttatgatg agtaaaattg gtttagcaaa tatgtgggga ttaatcttac tctatctcac
    12361 ttatgccgta ccacagacac tatttttata tgttggtttt attaaactaa atgtgcctat
    12421 cagtctggat gaggctgctg aaattgatgg ggctagtcac tttacaactt atttcaaaat
    12481 tgttttccca atgttaaaac caatgcatgc gacgactatt attcttaatg ctctatggtt
    12541 ttggaatgat ttcatgctac cattattaat tttaaatcgt gattctaata tgtggacact
    12601 accattgttt caatataatt atacgggaca atattttaac gactatggtc caagttttgc
    12661 ttcctataca gtaggaattg tcacgattac actagtttat cttgctttcc aaagaagtat
    12721 tatagctgga atgagcaatg gagcagtgaa ataaccaata aattattctc attataaagg
    12781 agttcataaa cttatgatta tatttgacaa tcacacacaa acatttcatt tacaaaataa
    12841 aaaaattagc tatctattag gaatcgaaga aaataattat ttaacgcatc tttattttgg
    12901 aaaaaagatt acgcactatt ctggtgggta tcgctacccc cgaacagatc gttccttctc
    12961 acccaacaat gcaaatgcgc aagatcgttt attatcttta gacacattac cattagaatt
    13021 tccaggatat ggacatggtg attttagaga accggcgcat aatattaaat taggaaacgg
    13081 tagtcgtgta aatgattttc gttatgatag ctacgaaatt caaaaaggaa agcccagctt
    13141 agcgggattg ccagccttat atacggaaga tgatgatgaa gctgaaacgc tgattattca
    13201 attgaaagac gcgattagtg gattgacttt acagttgttc tacacaattt atcaaaatca
    13261 cagtgtaatt attcgttcta cgcaattagt aaataatagt gatgaaacag tggagattaa
    13321 ccgcttagct agtcaatcaa ttgattttcc aaatcgtcca ctcgagttaa ttcatttaaa
    13381 tggtacctgg gcaagagaac gtcaaatgac tagagaaaaa attcatacag ggataaaagt
    13441 attagatagt aaacgcggat caagcagcca tcaacaaaat ccgtttgttg ctttagtaga
    13501 tgccaaaaca gatgaatttc aaggagaggc atatggattt aatttagttt atagcggtaa
    13561 tcacgaaacg gtgattcaaa aagatccatt tgaccaaaca cgggtcatta tgggaattaa
    13621 ttcatttaat tttggttggc aactaaaaac tggcgactca tttcaaacgc cagaagtcgt
    13681 gatggtttat agtaatgaag gtctgaatac gttatcacag acgtatcatg aattgtataa
    13741 tcaccgttta attcgtggaa actatcgttt aaaagaacgt cccacgttaa tcaacaactg
    13801 ggaagcaacg tattttgatt ttaacgaaga aaaaatcatg aatattgtcg atgatgcgaa
    13861 gaacttgggc attgaaatgt ttgttttaga tgatggttgg tttggcggtc gtgaaaatga
    13921 cttcacttca ttaggtgatt gggttgaaac agaaggcaaa cttgcttgtg gtttggaaaa
    13981 tttagccaag caagtgcatg ataacgggat gaaatttggg atttggtttg aaccggaaat
    14041 gatttctgaa gatagtgaac tatttcgagc acatccagat tgggcattgg gtattccagg
    14101 tcgtgaccgc tcaattagtc gaaatcaata tgttcttgat ttttcaagac aagatgttcg
    14161 tgataatatc tatcaacaaa tgacggcgat ttttgatcgt gtaccgattg attatgtgaa
    14221 gtgggatatg aatcgaaata tgacagaagt ctattctgtc ttgttggact caacgatgca
    14281 aggtgaagtg gctcatcgtt atatgttagg gttatatgaa tttttagaaa agttaacaac
    14341 gaattatcca actattttat ttgaaagttg ttcgggtgga ggcggtcgtt ttgatgcagg
    14401 tttcttgtat tacatgccac aaacttggac gagcgacaat accgatgccg tggctcgtct
    14461 gaaaattcaa tatggaacca gcttagtgta tccaatttca agtatgggtg cgcatgtttc
    14521 agcagtccca aatcatcaaa caggaagaga aacgagttta actattcgtg gcaatgctgc
    14581 gatggctggt gtttttggtt atgagctaga tctaacgcaa ttaacttctg aagaaaaagc
    14641 agaaattaaa gaacaagttg ccttttataa aaagcatcgc caacttttgc aatatggaac
    14701 tttttatcgc ttggaaagtc cgtttgaagg caatgatact gcatggatgt ttgtctcaaa
    14761 agaccaaaca gaagccatgg tcttttattt ccgagtatta gcagaagctg cgcatccatt
    14821 agtcacattg aaattagcag gattagcaga agaagcaact tatcaaatgg aagatgtaac
    14881 atttgctggc gatgaattga tgaatctcgg tttttatatt gactcagaac tacatggtga
    14941 ttatgcgaca caacgctttt atttgaaaca gatatagtag cttgaattta caatataagt
    15001 gcaacaccaa aagagactca ttccaaacga agaaaatatg ttttatttac acgcatttac
    15061 taaaaagcag cctgatttga attgggaaaa tgaagaagta cgtgaagaaa tctatgcaat
    15121 gattaactat tggttagata aaggactagg cggatttaga atcgatgcga tattaaacat
    15181 caaaaaaaaa ctagagtatg gcatctttga atctgatgga gaagatgggt tagccttcat
    15241 tgggccctgg attcttaatc agtcgggcat tgaagtgtgg ttaaaagaaa tgagagagcg
    15301 gacatttaaa ccacataata gtatgacggt agcagaagcc gatgttccta atgaacgttt
    15361 agatgaatat attggtgaag atggctatta ttcgatggtt tttgatttta gttatacgga
    15421 tattgatgta cctgaaactg gtgaatggtt taaagatagc caatggacat ggacggatat
    15481 gcgtaccaat atttttacga atcaattagt tacgcaagat aaaggttggg gcgccttata
    15541 tttagaaaac cacgatcaac cacgttcaat taataaatat attccagaag aatggatcaa
    15601 tgattatagt aaaaaaatgt tggcgacgct ttttatgtta ctaagaggta ccccttttat
    15661 ttatcaagga caagaactag gaatgaccaa catagaaatg gcatcattag aagattttga
    15721 tgatgtagct acgcattctc agtataaacg ggcattagaa tatggattgg caccagagca
    15781 agcattgaaa gctgtcaata aacgaagtcg ggataattct cgcacaccta tgcaatggaa
    15841 tacgcaaaaa aatgccagat tctcaacttc tgataatgta tggttaaaag taaatcctaa
    15901 ttatccagaa ctcaatgcag aagcttagtt aaccaatcaa cagtcggtat tcaattatta
    15961 tcatcaatta attaaattaa gaaaaaatag tgtatacaaa gacgtgctaa tctacggaca
    16021 atttctacca gtgaaagata aaaatgaaca tattttgctt tatgaacgcc aattagaaga
    16081 taaaatcgta ttagttatct taaacgttac agcaacagaa caagaatatt atgtagatga
    16141 acagtaccaa caagtattaa tcaataatta tgaagatgtt ttattagatc aaagtcgaaa
    16201 attgcgttta cgaccgtttg aaagtattgt attagctaat tatggaggac aattatgaaa
    16261 attaaaaacg aagcaatgct catcacctat gcagatagtt taggaaaaaa catgggcgaa
    16321 ttaaaacaag tcttagacaa acatttacaa ggtgttattg gtggtgtaca tctgttgcct
    16381 ttttttccat cgacaggtga tcgaggcttt gcgccgagtg attatacgac agttgatcca
    16441 gctcttggaa catgggaaga tgtggaagca ttaggtgaag attattattt gatgtttgat
    16501 tttatgatta atcacatttc tcgtgaatca aaatttttcc aagatttcaa aaaaaaccat
    16561 gagcagtcac catacaaaga aatgtttatc cgtattcatg aatttttccc agaaaatcga
    16621 ccaacacaag aagatattga tttaatctac aagcgtaaag acaaagcgcc gtttcaaacg
    16681 gtagaatttg cggatggtac gacagaagag gtttggaata cctttgggga agaacaaatt
    16741 gatttagatg tgaccaaaga agttgtgaaa gaatttattc gagaaaccat caaagatatg
    16801 gcgaaccatg gctgttcttt aattcgatta gatgcgtttg cttatgcgat taaaaaatta
    16861 gataccaatg atttcttcgt ggaaccagat atttgggaac ttttagacga agtgcgtcaa
    16921 gaagcagcga aatatgacgt cgaattatta ccagaaatcc atgagcatta ttccattcaa
    16981 atgaaaattg ccaatcatga ttattatatc tatgacttcg ccttgccaat ggtcactctg
    17041 tattcacttt atagtggaaa atcagaacgt ttagccaatt ggttgaaaat gagtccgatg
    17101 aagcagttta caacattgga tacgcatgat ggaattggtg ttgtggacgc tcgtgattta
    17161 ttaacagatg aagaattgga ttacacctct gaggaattgt ataaagtcgg ggcgaatgtg
    17221 aagaaggtct attcaagtgc caattacaat aatttagata tttatcaaat taacagcacc
    17281 tattatagtg cattaggcga taatgaccgt agctatctgt tagcacgtgc gattcaagta
    17341 tttgcgccag ggattccaca aatttattac gttgggttat tagcaggaaa aaatgatatt
    17401 gaactgttgg aagcaacgaa agaagggcgt aatattaatc gtcattacta tgatttagcc
    17461 gaaattgaag aagaagtgca acggccagtg attcaacaat tatttgcact tttaaaattt
    17521 agaaatgaat ctgcggcgtt tgatttggat ggaacgattg aagtagcaac gccatcagaa
    17581 tcagaaatag tgattacacg taaaaataaa aacggtagtg atagtgcaac attaacggct
    17641 aacttggata cgaaagaatt tatcattgag tctaaagata taaaaatttt cactagtgtt
    17701 gcattaaaat tttaacctat tccatagaac aaaaaatcct ttatactagc acttggatga
    17761 cttatccaag accaataaaa aggactcata catggataag tataaaacaa atacagcatt
    17821 taataaatgg ttttctgcca taaaattagg aaaactttct gtcaactccc aaaaatgtat
    17881 ttttgattac aattcgtatc gtaaaaaact gtcttttgaa gctgtgctca agctctttct
    17941 ctacgcagtt aatgaagaaa aagaaagttt gcgtgatttg agtacagctc ttatcgaccg
    18001 atcgctccaa ctagaaaccg atgtgtcatc gattagtcat acccaattgt ctcgagcatt
    18061 taatgcactt gaacctaaag tgttagaaga aatttttcaa caactgttag aaaaagttca
    18121 acatcaaatc aaaccaacca agcgaaatca tctgtatctg attgattcca gtacgttttc
    18181 gcttagttta aaacgtcata agtgggcgac tttccgtcaa acaaaatcag gtgtcaagtt
    18241 acacttaaac tattgttata tggacgatac tacgatgtat ccaactgatt ttaccttaac
    18301 aaatgcccat gaacatgatc tcaatcaact accagtattg gtgaatcaac cagaggcaac
    18361 ttacgtgttt gatcgtgggt attaggactt tgaaaagatg gatcagatgc attgggatgg
    18421 ctatttcttc gtcactcgga ttaaaaaaaa caccaaagtt cacgtgatag atactctgga
    18481 aacatcgcca gaaacagaaa ttctacgaga cgagctggtt cgtttaggat ctaaaaccta
    18541 tctaaccgcc aatttccgac tagtcactgt tcaagataag aacggacgtg tgtttcaatt
    18601 tatcaccaat cgaatggacg tctcttcaaa agaaatctct gatatgtatc acgctcgttg
    18661 gcaaatcgaa ttgtttttca agcacatcaa acaacacatg accattaaaa ccttcttttc
    18721 tcaaagtgaa aaaggcgtgc agaatcaatt gattttaacg atgatttccg ctctcctcac
    18781 gtttctcatc aaattggaga cgaaaacgga aaaatcagtg ttccaaatca aacgattctt
    18841 tcgttattta ctgtttcagc cctttgaatg ttgtctagaa aaattaattc ccacatgaga
    18901 aaaaagaaat ttggctcgtt acttttggtt ttcagttgaa taacagataa aaaattttct
    18961 ggaaaaagtc atcctctgcg tcagcacagt tttttttgac ttattttcag aggaaatagt
    19021 tttcagaata ttctaaaatg ttttatgcaa cactagtgat attttttaga attagatttc
    19081 taatgtgcat tttttgagta ttaaggagca agtatacaaa gatttataaa gctgattaaa
    19141 tttgaataaa aatatgcagg aacgctacta tttttagtag tgttcttttt tatatttgaa
    19201 taaaaaatgt ggtggttaaa tggtggttat aacaaaaaat gaattaatga aattaggtta
    19261 tgggaagtat cagtctgaga gaatcatacg acaggcaaaa gcaattatgg cttcaaaaag
    19321 ctatacctat tacgaaaata gaagattagg actagtacct gttgaatctg tagaagaagt
    19381 tttaggtatg aagatagagg tagaggagga ttttcagaat catggttaat tttaaaaaag
    19441 ataaaaatgg gaaatatcat tttactgcta gtttgggatt cgacaaacga acagggaaaa
    19501 gagtacaaaa aaacgtagag gggttaaaac agttaaagaa gaaaaaaagc gtataccgaa
    19561 cttgtttcta attatggaaa agaagcagaa acttctcaaa gtactatgtt gtttgaggag
    19621 tttttttata aattattcct accttattat caagggaaag tagaagaaag aacatataaa
    19681 aatcggttat ctacaatcaa aaaaactttt cttatttctt taaaatggag ttaagagaaa
    19741 tacgtccaat tgacattcaa aagtggcaaa ataatttatt gagcaaatat aaaaattctt
    19801 atgtaagaaa catgtatggg atttttcaga tgtcattaga tcgagcagtt gttttgggac
    19861 tacttgaaaa aaatcctgca aaagttattg gaaatgtgaa gaaaaggaaa actacagttg
    19921 atttttggac aaaagaagaa tttacaaaag ttatttcaac tttttatatt gatgatttgt
    19981 accaacattt tggttttatt tgtatgtggt tactttttat gaccgttatg cgtttgggag
    20041 aagccactgc attaacttgg aaagatattg attttgaaaa taaaaacttg cgtgtgaata
    20101 aaacactcta ttataagaga gccaaaacct atgagtttgt agaacccgaa acggcttcta
    20161 gtattcgtac aatcgaacta gacgacaata caattacctt tttaaaagct tggaaggagc
    20221 gtcaagtttc aataggaggc atgtcgtttg tactttctta taatggaaca cctactcaga
    20281 aatatacaat ccgtcatatt atgagaagac atgcaacgat cgttggtgtt catataatcc
    20341 gcatacacgg attaaggcat agtcatgctt ctttgcttat atcacaataa aaagatgcac
    20401 ttcgaattaa agaaaggtta aaacatgctg atgtacaaac gactttagga acttatggtc
    20461 atttgtatct aaattcattg agagagataa caaatgatcc cacagattat atccaaacta
    20521 atttttctaa aatcaaaccg acacctattt acgataataa aaaataaatg ataaacaaaa
    20581 gtaggatcac accaaaagaa acacactaga aaaactataa tacaggcttt tctagcgtgt
    20641 tgtttcttat ttccgcctat ttatacatta tactcataaa gcatttaatg catcgatcgt
    20701 agccgcattg tctatatggg tatataaagt tgttgtattg gtacttgaat gtcccaattg
    20761 ttgggcagtt aataccaatg aatttgtctg ttggtagagt ctagtagcta atgtatgacg
    20821 cagtttgtga gggctgactt gaattctaaa gccttttgaa tattttgcta ccatccgttc
    20881 gattgcaata ccggatatac gcttggcttc tccttggtaa attgctagaa ataaagcttt
    20941 ttcgttttct gttactttat atcgttcttt ccgaatagct agataattag ctaaatattc
    21001 cattgcgaat gctgctatat tgacagcatc tcttttattt ccttttctag ttactaccac
    21061 attattctta tctaaagata ggtcatctaa attaatattt ataacctcgg agagtctcaa
    21121 tccagatcct aaaatcagtg cattgatcgc caggtctctt tctacatctc gttgataata
    21181 gatcaaagct cgtttactca aagtattttt atattcattg gcaatataat ctagataatt
    21241 ttgtgtatcc ttatctagaa ataatttttt ctcaatggca ttggcacgtg cagctaacgt
    21301 agttttatct gtgatggttg ctacttgatc cataggatta ttgtctaagt atggttttcc
    21361 tagaggatta ttggaacttt tcgaaagata tttaaataga acctttaatg ccgtgatatt
    21421 tctattgata gtagaagtag atagacctaa gttattatta tctttgagct tagtttttgt
    21481 tgtctctttt tttacatttt cttttgtgcg acgtttcaaa tgagatttgt acaagtttag
    21541 gtcataggca gttaactgtc ttatcgaggt gatagaagta tcaggaacga tagactcact
    21601 cagaagccat tcataaaaga gcttataatt ttttagatat tctaaagtgg ttcgaggtga
    21661 ttgatcttta tcctcacgat cttgaacaaa atcttttata taatcgggca tatgaagtat
    21721 gattgtatct attttttgat atatttctgt atttttcatg tcattcatcc tttcaataag
    21781 taggggagaa gagtatctct ataattggac tataccaatt atagaatgtt ttgtcaattg
    21841 gtatgctcta aaatttatta atttgtttca aaaaaagcat
//
LOCUS       DASJTC010000049        21571 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.049, whole genome shotgun sequence.
ACCESSION   DASJTC010000049 DASJTC010000000
VERSION     DASJTC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.049"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            256..468
                     /locus_tag="U4B19_001611"
     CDS             256..468
                     /locus_tag="U4B19_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2077353.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            471..683
                     /locus_tag="U4B19_001612"
     CDS             471..683
                     /locus_tag="U4B19_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077354.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            823..978
                     /locus_tag="U4B19_001613"
     CDS             823..978
                     /locus_tag="U4B19_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077355.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(975..1199)
                     /locus_tag="U4B19_001614"
     CDS             complement(975..1199)
                     /locus_tag="U4B19_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN2077356.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            1277..1465
                     /locus_tag="U4B19_001615"
     CDS             1277..1465
                     /locus_tag="U4B19_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077357.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            1791..2126
                     /locus_tag="U4B19_001616"
     CDS             1791..2126
                     /locus_tag="U4B19_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077358.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            2359..3300
                     /locus_tag="U4B19_001617"
     CDS             2359..3300
                     /locus_tag="U4B19_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEN2077359.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            3302..4192
                     /locus_tag="U4B19_001618"
     CDS             3302..4192
                     /locus_tag="U4B19_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEN2077360.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            4235..5035
                     /locus_tag="U4B19_001619"
     CDS             4235..5035
                     /locus_tag="U4B19_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEN2077361.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            5050..5901
                     /locus_tag="U4B19_001620"
     CDS             5050..5901
                     /locus_tag="U4B19_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2077362.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            6102..6563
                     /locus_tag="U4B19_001621"
     CDS             6102..6563
                     /locus_tag="U4B19_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2077363.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            6589..7041
                     /locus_tag="U4B19_001622"
     CDS             6589..7041
                     /locus_tag="U4B19_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077364.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            7147..7341
                     /locus_tag="U4B19_001623"
     CDS             7147..7341
                     /locus_tag="U4B19_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077365.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            7372..7758
                     /locus_tag="U4B19_001624"
     CDS             7372..7758
                     /locus_tag="U4B19_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077366.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            7755..7958
                     /locus_tag="U4B19_001625"
     CDS             7755..7958
                     /locus_tag="U4B19_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HEN2077367.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            7955..8251
                     /locus_tag="U4B19_001626"
     CDS             7955..8251
                     /locus_tag="U4B19_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077368.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            8327..8743
                     /locus_tag="U4B19_001627"
     CDS             8327..8743
                     /locus_tag="U4B19_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEN2077369.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            9004..9141
                     /locus_tag="U4B19_001628"
     CDS             9004..9141
                     /locus_tag="U4B19_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077370.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            9551..9781
                     /locus_tag="U4B19_001629"
     CDS             9551..9781
                     /locus_tag="U4B19_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077371.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            9940..10773
                     /locus_tag="U4B19_001630"
     CDS             9940..10773
                     /locus_tag="U4B19_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HEN2077372.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            10766..12181
                     /locus_tag="U4B19_001631"
     CDS             10766..12181
                     /locus_tag="U4B19_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEN2077373.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            12193..13722
                     /locus_tag="U4B19_001632"
     CDS             12193..13722
                     /locus_tag="U4B19_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEN2077374.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            13640..14692
                     /locus_tag="U4B19_001633"
     CDS             13640..14692
                     /locus_tag="U4B19_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEN2077375.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            14694..15011
                     /locus_tag="U4B19_001634"
     CDS             14694..15011
                     /locus_tag="U4B19_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077376.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            15138..15761
                     /locus_tag="U4B19_001635"
     CDS             15138..15761
                     /locus_tag="U4B19_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEN2077377.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            15776..16666
                     /locus_tag="U4B19_001636"
     CDS             15776..16666
                     /locus_tag="U4B19_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HEN2077378.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            16689..16904
                     /locus_tag="U4B19_001637"
     CDS             16689..16904
                     /locus_tag="U4B19_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077379.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            16916..17257
                     /locus_tag="U4B19_001638"
     CDS             16916..17257
                     /locus_tag="U4B19_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077380.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            17257..17628
                     /locus_tag="U4B19_001639"
     CDS             17257..17628
                     /locus_tag="U4B19_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077381.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            17621..18016
                     /locus_tag="U4B19_001640"
     CDS             17621..18016
                     /locus_tag="U4B19_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEN2077382.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            18018..18395
                     /locus_tag="U4B19_001641"
     CDS             18018..18395
                     /locus_tag="U4B19_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077383.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            18396..19004
                     /locus_tag="U4B19_001642"
     CDS             18396..19004
                     /locus_tag="U4B19_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2077384.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            19004..19345
                     /locus_tag="U4B19_001643"
     CDS             19004..19345
                     /locus_tag="U4B19_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077385.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            19587..21571
                     /locus_tag="U4B19_001644"
                     /pseudo
     CDS             19587..21571
                     /locus_tag="U4B19_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT         7862 a         3122 c         4528 g         6059 t
ORIGIN      
        1 aaataccgac aaaaaaagta tttttctgca aattatctat ttacaaatag agaatttgca
       61 gttatactag ttttgtaagc aagatattta cggcatacaa agactaaaag caataaacta
      121 atagtctccc cagacatcag taagctcgct ttttgtgcgc tgtattttgt atgcttacat
      181 aatagcatat ttgcagtttt tgtcaaactt attagacaaa aatattagaa aatattcgat
      241 aagggggtat gaagcatgac tttaaaacag caagtattaa ttgctttagt aaagaaaggt
      301 tggagtcaaa gagagttagc tcgacgaatg ggtatatcaa ttacttattt acgagatatc
      361 ttcatcgaaa aaagaaaacc taaggaacgt ttaaaacaaa tcgaagaact tttggatatc
      421 aagttagaag tagattctaa tgatcagcca tcagaacagg aggcgtaaaa atggtacaac
      481 gtttgagcaa agcggagttt aaagagaaat ataagtattc tgaaagtact tatcaaagaa
      541 gaatgaagga atttaaaaaa tcacgtttta gtgaggggta taacgcagtt acttccaaag
      601 aaataacaat tgatgtcgat ttatatgaac agtttagaac atggcgttct aaaaatcgat
      661 ttagagttag agctgtttgt taatagctaa aaaatctaaa ttcaccactt ctgaaaagag
      721 gagttgctgc tctcctttca agtttgccaa agtccacgtt ttttcatttt ggcaactcct
      781 cttcttggag gtggtgacgg cgagagacga aggagggatg acatgaagga agaatcaaaa
      841 atagggattt tgaatgagaa caattacaca agattaatta ttgaaacaga agaagggaaa
      901 aaagtagcag aagttacatt gaccgatgca actcctgctg acggttatcg tattcgatta
      961 actcctaaat atgattaacc ttttggtggg tgtggatcgt ttccgtgact atcacggctt
     1021 tgaatttttc catctctgcc atgaataaat agctcagatc cttgattttt agaaatctct
     1081 ctagcgattt ttactgcttc tgattttgta ttagtatgtg cagtcgcttt agaatttcca
     1141 gcacctttga cattccaacc accattggaa ttaggtacaa catgttgatt ttttcccata
     1201 gttttcacct ccatttctat agaaatattt tatcaaatag aaatggagaa attcaataaa
     1261 cgaaaggaga agtgatatgg catatacctt gcaacaagaa catcaaattc tcggtttgat
     1321 taaacaacgc aggaaacaat tacaagatga ccgtgcagcg cttagaaaag ccgatgagct
     1381 atcagatagg caagctgaac taattgcttc tgaacttgag gatttgagaa tgctagaaat
     1441 aaaaaatagg gagattagat tatgaagaag acagacacac tttttatagg attcattttg
     1501 gggttattag tgattgtagc gcaccaaagt attatcgggg gaagcttgtt cgcagcattg
     1561 atggttttaa tcaatctgct tgattcaaaa gaaaggagca actatggcac gagaagaagc
     1621 gctaaaaatc ggtaaagtga ttgctgataa tcggtgggga aatagccgcc ctattatttt
     1681 aagcaagcaa catatcgaaa agcaaaaagc atggcaacaa ataaaaaagt gactctgcca
     1741 gccagcaaag agtcacatac aaatttataa aactaggaga attatatcac atgaataaga
     1801 aaattgaaaa tcttatcgaa gaattgaaac gtgaatgcca aaagcaaggt gtttctatta
     1861 tttgtacagc ccaaaaagaa ggggaactta aaagtctcgt ttatggtgag acaacagaaa
     1921 ttttactttg tcttgctatg caagaagaac atttagatga aaatctccca ttgtcagcac
     1981 atattatgcg tagaattgct gtggatgctt acgaacaagc gaaaaatgaa gaagaaaatc
     2041 aaccttcaaa tcatactttt gttattaata acaaggaaga tttagctgat gtgatgactc
     2101 gtattctcaa gggggaattt caatgatgaa tgaaaaaaca attgatctac tcattatttt
     2161 atttttcacg ttagtgattg tagcgaaaag ttatgtaatc tttggcttgt tattcgcaat
     2221 taccatgctt gtgctttgcg tgttagcaag cagaaaagag gttaaacatg aacgagttag
     2281 caaatcttga caactattta actgatcctg attatgctaa accaccatat gaagcaccaa
     2341 ttgatgagga ggatgaagat gagtaaatct accttagaaa tgagccatca agaatggctt
     2401 gaagaccgta agaaaggcat cggaggttcg gatgttggaa caattttagg attgaataaa
     2461 tggaaatctc cttatcaact atggctagaa aaaacaggac aagtagtact tgaagaatca
     2521 ggaagtgagc ccgcttattg gggtaatgtt ttagaagaag tggttgctaa agagtttcag
     2581 gaacgtacag gcaaaaaagt acgcagaaga aaccaagtat ttgaacatcc attacatcca
     2641 tttttaagag caaatattga tcgggaagta gtgggagaaa atgccattct ggaatgcaaa
     2701 acagctaatc aatttctcgg taaagaatgg gaaggcgaag aggtaccgct tagctatctc
     2761 tgtcaagttc aacattacat gaacgttcta aacaaagact attgttacat cgctgtctta
     2821 atcggtggtc aaaaatttat ctggaagcgg attgaacgag atcaagagct gatcgataca
     2881 atcactgaac aattagtaga gttttgggaa acaaacgttc ttggaggtat cgagccaatt
     2941 attgatggta gtcaagcgac agctgatttc ttgaaagaga agtatgcaga tgttgaagac
     3001 gttcaaacga cgttaccaat tcatttcgat gaactagttg aacagaaaaa cgaactcaaa
     3061 cggactaaga aagaaattga atcagctatc cgtcaagtag acaacgagat tatcagcgag
     3121 ctaggtaaac gtgaagccag tatcggtatc actcaaaaaa acatcatcag ttggaaattt
     3181 gtccgtacga gacgtatgaa ctcgaagaaa ctagcagaga aatatccaga tgtcgcaaat
     3241 gatgaagaga tttataacgt tactgaatca agaaggctaa ccgaaaggga gatcaaataa
     3301 tatggcaaca aatgaatcgt taaaaaatca attggcagca aagccacaga aacaagttgc
     3361 accaggtcaa ttaggactta aagctttgat gaatacacca acaatgagaa agaagttcga
     3421 agaggtgctt catgacaatg ctaatgcttt tatgtcgaat gttatgactc ttgtatctaa
     3481 tgacagttat cttgcagata gtgaaccgat gtctatcatg agtggtgcgt taactgctgc
     3541 aacattaaat cttgggctag ataagaattt aggttatgca tatttagttc cattcaatag
     3601 taaaaacaag caaacaggaa aatgggaaaa gaaagctcaa ttcatgcttg gctataaagg
     3661 atatatccaa ttagcccaac gatcaggtaa atacaaagca ttaaatgtga tcgaagttta
     3721 cgaaggagaa ctaaaaagct ggaaccgact gacagaagag tttgagtttg atccaaatgg
     3781 tagaacgtct gatgaagtca ttggatatgt tggctatttc gagttactga atggattcaa
     3841 aaaaactgtc tattggacca aacaagaaat cgaagctcat cggattgcta acaataaaga
     3901 tcgcgataag acaaagttaa gtggtgtgtg ggcatctgat tacaatgcaa tggcacgaaa
     3961 aactgttttg agaaatcttc tttctaaatg gggaatcttg tccatcgaaa tgcaagaagc
     4021 caccacatcg gatgagagag tccaaagggt tcaagaagac ggcagcatta ttgctgaaac
     4081 agaagttgaa gaagatattc ctgaaagaaa agaagcagag gttatatctg aagaaaatga
     4141 agatgtacaa actggattat ttgatgcatc taatccgccg ttaaacaaat aatgagggag
     4201 ctttctccct tgcttttcta gaggagaaat acgaatgaac acaggatatg tcaaattaca
     4261 tcgaaaagtg atgaattcat tcgtttggac caatccttat atgtataaat tatggaactt
     4321 gtgtttgatg aaagcgagtc atgagaatcg caagatactt tttaatggaa aagagataca
     4381 agtgaacagc ggagaattcg tcacagggcg cgatgctatc acatctgaga tgaacgaagg
     4441 cgtgaaacgt gaacatcaag tgaacagcgg ttctgtatgg agatggctaa aacaatttga
     4501 aaaaaacgga atgttgaaca tcaaatcaac tacgaaatac agcgttatat caataaaaaa
     4561 ctggtctttg taccaaagca gtgaacaaca aatgaacatc aaacgcacaa caagtgaaca
     4621 acaagtgcac acaaacaaga atgaaaagaa ttataagaat gaaaagaata ataaaacatc
     4681 atcgtcgcaa ccacgcaaaa agcgtgttta cgacaccgac tcagtttact acattctcgc
     4741 ggaggagtta ttcaaacaga tttgtcagaa tcaggaaatc aaaaagccga atctgcaaag
     4801 ttgggcagat aacattcgga aaatgatcga gatcgacaag cggaccgaaa accaggtacg
     4861 gggaatgatt gaatggagtc agcacaatgt gttttgggca tcgaatattt tatctgctaa
     4921 aaaattgcga gaaaaatacg acacaatggc agcgcaagcc aatcgtgatt ataaaacaaa
     4981 acaaactaaa acgcttgaat acgagaaatt tagcacagat gagttgccta tttgagagga
     5041 ggcgtaggca tggaaactgt tggggaaatc atggaaaagc tgatacagaa agtgcttgtc
     5101 caacgtggcg aatgtcctga atgcggacag cctttgtatg gatggcgtac gaagaaccct
     5161 gatggctcag aacgttgtaa gccaacctgt atgcaatgtg ggtataaagc attgcgagtt
     5221 caggaagact tacaaacaga acgaatttac aacgagagcc tgaaagcaag agcaatcaat
     5281 tttttcaaag gtggttctgt tgtgcctaat caagcgttgt ttgattgcac attgcagaat
     5341 tatcaaattg tcgatcaaga aacaagacaa gcggttgaag taaccaaacg ctttgttaat
     5401 tcagtcttgt taggaaatcc aagtcacctt gttttaactg gaaaacaagg aacaggtaaa
     5461 agtcacctag cgatggcagc ggcttgggaa gtcttgaagc gatcaaacta cgataaaaaa
     5521 atcttattta tcgggttaca ggaaatgctg gatcaaatca aattttcgta caacaatcct
     5581 gaactcagaa aaacgattga gggatcgttg attgcagata tcaaaacagc ggatttagtc
     5641 atcattgatg atatcggttc agaactagga aaagatgcat cagatagtcg agcgtttggc
     5701 ataaacacgc taaattcgtt cttggatgca cgacagaacc tagcaacgat tatcacaacg
     5761 aacttgcttg gtgaagaact gaaaaaagct tacggtacga gaacaatatc aagaatgttt
     5821 gtcaactctg atggatttac gatggtattt tctcaaacag cagacaagcg cataaaacca
     5881 gtcaaaggag cgatcgcatg aatttgacta aacaagaaaa aagaatctgt cgtcagataa
     5941 tgttgcaaat tataggtgat cgtgacatgg acgaattatc tcaacaagag agtcgaatgg
     6001 ttcggaagat aagtaagcta attatttatc gggatacaga aagatttctc actgttttag
     6061 gtaacgtcta cgagaatcca gagttattgg aggtagagta attgccaaaa atattagatg
     6121 catgttgcgg tagtagattg ttctggtttg ataagaacaa ttctgacgta acatttatgg
     6181 attgtcgtca gcagtatgaa gaattatcaa caggtcatgt gatcaatgtt gatccagatg
     6241 tggtcgcaga ctttcgcgat atgccttttg aagatgatgt gttcgatatg gttgtatttg
     6301 acccgccaca cttaagacat gtcggcgaaa actcatggct tgccaaaaaa tatggaaaat
     6361 tagacgaact ttggccagaa gacatcaggc aaggttttgc agagtgtatg cgagttttac
     6421 gaccttcagg aacattaatt tttaagtgga acgaggaaca gataccactt tctgatgtgt
     6481 tagaggctat tggagaacag ccattgtttg gtaataaacg aagcaaaaca cattggcttg
     6541 tgtttatgaa aaaattcagc taacggagga aacaaaggag gaaaagtaat gaagattgta
     6601 ttaaataaat gttttggtgg ctttgggttg agccatgaag caaaaatgga aatttttaaa
     6661 agaaaaaata ttgaagtatt cccatacatc aataacttta gctatgactc agatgatgaa
     6721 tacacgagat acacaggcca aaagctaggt tcgatggatt ttatctacta tttcaaaaaa
     6781 gacccgaaaa tcgacaaagt tacaggaatt tatagcgaaa ttgaccgact gtacggaatt
     6841 gctgatgatt ccagtttttc aagtgattca aacagaggtg ataaggacct cgttgctgtt
     6901 gtcgaaaaac taggaagtga ggccagtgga ccatatgcaa gtcttaaagt cgttgatata
     6961 cccgatgggg ctgagtggga aataagtgat tatgacggcg ttgaaactgc acattatggt
     7021 tttcaaacag gtagtattta gtccactaat gacaactaaa gaggaggcag aaggatgagt
     7081 gaagatgaat actttgaaca gtagtctatt aacgacagag atttttaact ggaaaggggt
     7141 gtagtcatgg acgcatcgga agtagtaaat aaaattaata gtctcaaaga ggttttcgga
     7201 gacgtcgaag tagtgataaa cgcacaaggc tatgacaacg gattttttaa gcagattacc
     7261 gacattagca ttcagcaagg aatggaagac gaagatggaa actttatcga tgaagtagcc
     7321 attttagtga cttgcgagta agtaattgac agctaaagag gaggcagaag gatgaaaaaa
     7381 ttgcaccaac tcatatctga gaaagaaagc gaactccaaa atttagaaga tagtttggga
     7441 ttggggtttc caattgttga gcaagctaaa atgactcaaa tcagtcatct acgacttgaa
     7501 ttggaagatt taagacaaat agaaaaatca attcaactca acgacaacca gaaaatcgtg
     7561 ttggagtggt tgaaaagcga gacaatttta actagagaag caccaatatt atctgttaat
     7621 gctttttctg ataaaaattt actaggaaaa ttacctgata aagtacgcaa agcttataaa
     7681 ctattggctt gtaaacaaga atatgaagtc ctatcagcat tcgctcaatg gggattagaa
     7741 caggaggaag cggaatgaac aacagacatc gcagaataac aaaactaaga aaacaggaac
     7801 tgaatgtact aaagacaaag tttgaaaaag aatatggaat ttcagcagaa gaaacatata
     7861 aagtggcaag tcagtttgtt gctgatgcga gtaatgctat tcgtaagttt gggatttcga
     7921 tattaaatga tgatcgtaaa tgggaggaaa tgagatgaaa ataaaagacg gattttacgc
     7981 tagtagtcat ggtatcggcg gtttaatgct agacatgccg ataaagaacc ctaaaacacg
     8041 tcagaaatca aaattcaaaa tcggtgacat ggtccgatgt aaagcagaag agttcatcta
     8101 tccgtttcgt ggatatgtag agcatgtcta taatcattca gcaatcattc acattgaaaa
     8161 cacgatggaa tgcgataagt ggttagcgaa aagcaaagat aatttagcag tggctcgttt
     8221 ggtggatatg gaactaatca atgacaaata aaaaagccgg atcgctccga ctgattcaat
     8281 aaatccaaca catttattat atcacataaa ggagcggttt gacttgatgc aattgttacg
     8341 agaggtagat ttcaaacaga caagatgtaa tgcgagagat gtgctgaaga actttcggcg
     8401 tttggagcgg atggcaggtc gctctttgat agatattaag tcgccgatta ttacggatat
     8461 gccgaaggca ccgaagcacg gcaataaggc agaagacgcg atcattcaga tgatggatat
     8521 agaagcggag agagacgcaa tactagcggc tttgatggct cttagtctga ttagccgtca
     8581 gatactctac tacagcttct gtgacgtaaa caagcactct aattatgaaa tagggcaatt
     8641 gatacgagga tacggagaga agaatgtaga gaagttgaaa tccatcgcat taatcgaatt
     8701 tgcagaagca tacaaaaaag gcgtgttagt tcagtatcgt tgattttgta gggtttttgt
     8761 agggatagtg tagggttttt gagcggttta acgtgatatt atggtagtgt cgaaagatta
     8821 gtgataggtc tgagacaaaa taaaatgtaa gggaggaaat ctccctcatc gttttaactt
     8881 aagcttcgat agacagcaac ggaaatatta agaataagga tgtgaatttc aactccttct
     8941 aaattgttct tattatctat catccgttgt tgtctattaa tttatgcatc ggaggaaaaa
     9001 cgaatggata aagaaatcaa agtaactgtc aaattagatt tgactgaact aaaagaactg
     9061 ctcaacaagg ctagtgacca agtcgaacag ttacaagaaa ctttagatga aattgctaat
     9121 tttaaaatcc aagtttctta gcagtatatt ttttagcagc tgcactcata aaatcatacc
     9181 aagtatcaga aaaatataac ttaactaaat aattaaaaaa ggtgggtaaa tagaaatgac
     9241 ggcgttaata atttcaattt ttgcgctctg tcttaatgtc tatatgattg gatttaaaaa
     9301 tggacaaaat aaaaaatagt agcagccgag aataatttta tagtgtcact gtggcggaaa
     9361 tagaagacgc aaaggtggag gtaggaagcg tatggcgcac tatcgggacc ttgtacaaaa
     9421 ctagaaagga ctagtacgtg tgtggtgcga gccctaggga ggaacaggat aaccgcctgt
     9481 gtgtaggttg ctattacata ttagatcact ctttgagtgg tctttttatt tacataaagg
     9541 aggctacata atgagaaact actggtatat atcgctaact aatgaatatc ctcgaaccat
     9601 tgatgattgt tcagtgcgtg ttgtgcgttc tgtccaaatc aaaaagaagt actccattgt
     9661 tgaaatgacc agagaagcta cgccaaaaga gattgataaa tgcaaacttc tttattgcgg
     9721 tcatggtttc tatgatgaac caaacattca aaataatatt aacaagaatt tgagggatta
     9781 gaatgcaaga aatggcttaa aaatctccaa tcaggacaaa tcaagttaga caaagtttcc
     9841 gatttaaaga tattgattga agctgattta atgctaaaag atattgaaaa ttagaaaaca
     9901 aaactcaacc taagaagatt gcgaggtggt gtgtattgaa tggcaagaca acgtgatcca
     9961 agacgtgatg aagccaaaag aatttggtta gaatccaacg gagaaaagca gttaaaggaa
    10021 attgcatctg aattaaatgt ttcagattct caggttagaa aatggaaatc gcaagacaaa
    10081 tggagcgctg aattgaaaag taacgttacc aatggcaaaa gtaacgttac taatcaaggt
    10141 ggcgctccta ttggtaatca aaatgctaaa ggtaacaaag gaaacagcag agcatcaccg
    10201 ccaaagagaa ataagaatgc tcttaaaact ggtgagtatg aaacaatatt ttttgacacg
    10261 ttaaccgata atgagaagga catctattct agtttgaatg atgatccttc ttttgctttg
    10321 tccgaagaaa tacgacttct taaaataaga caacttcgaa tgatgaaaag aatccaacaa
    10381 gctgaagctg gactaaatga tgaagaagtc gaacgattgc agcagctaag aaagattaaa
    10441 actccgattg aaaaaaatgg taaaaagcta gaaatcaagc gtgaggttat gcaagatgtg
    10501 cagattagca gaaaaaaaca tcgcaaaatt gatgatattc tttcaattga agattcattg
    10561 actcggatta gcaaccagtt agctaaagcc atcaagcaaa tgaatgaact ttatatgaat
    10621 gaatacagaa ctgatttaat taaagctcag actgataaga tccaagctga gacaaatgaa
    10681 attggcggaa ataattcagg tgaagaaata gaagaatgga aacaggcagt tttaaatgcc
    10741 gcaaacaaac gggcggtgaa agaaaatgaa taatgaattt attccttttg ctgatattgg
    10801 ttctgccatt gattattact atgataaacc agtagctttt tgccaagata ttttgcattt
    10861 gaatcctgat gaatggcaag aaaatgtttt aaatgattta gctgaatttt caaaggtttc
    10921 tgttcgttct ggtcaaggag ttggaaaaac agcattagaa gcaggagcaa tactttggtt
    10981 cttaacgtgt cgaccctacg ctaaagtaat agcaacagct ccgacaatga agcaacttta
    11041 cgatgtactt tgggcagagg tagctaaatg gttaaatgat agcttgatca aaaacttact
    11101 gaagtggaca aagaccaaaa tttatatggt tggtgattca gagcgttggt ttgctacggc
    11161 tagaacagcg actaaaccag aaaatatgca aggttttcac gaggaccata tgttgattgt
    11221 ggtagatgaa gcttctggtg tgtctgatcc aattatggaa gctattcttg gtacgctatc
    11281 aggttttgat aataagctgt tgatgtgtgg aaaccctaat aatattgaag gtgtctttta
    11341 cgattcccac aattcagacc gtgataaata cagagttcat aaagtatcaa gctatgatag
    11401 taaacgtaca aacaaagaca atatagaaat gattcttaaa aaatatggaa aagaaagtga
    11461 tgttgctcgt gtccgtattt ttggagaatt tcccaaaggt gcgttggatt catttatcag
    11521 tcttgaaacg gttgaattgg ctacagaaaa acaaattagt gattctttag tcaataaaac
    11581 aacggttgct catattggtg ttgacgtagc tcgatatggt gatgattcta cgattctctt
    11641 tcctagaatt gctaccaggg cattggagta tgagaagtat tcaaaacgta gcactatgga
    11701 aacaacagga tatgtcatca acatggccaa gaatctaatg agtcaatatc cgagtattga
    11761 taaagtgatg attaaagtcg atgacactgg tgtcggaggt ggtgtaaccg accgcctaga
    11821 agaacttata gaagacaagc attatccttt tgaggtgttt ggagtgaata acggttcaac
    11881 atcagaagac gatttttacg ataatttagg tactcaacta tggggaaaca tcaaggaaat
    11941 gttagaagaa aatatgacag caaatcttaa cggagaacag cctgttattg aattgccttc
    12001 tgatagttcg ttaatcaaag aattaagtac tcgcaaattc aaaatgacaa gtagaagtcg
    12061 tatacgttta gaaagtaaag atgatatgaa aaagcgaaat attggtagtc ccgatattgc
    12121 tgacgcactg gctttagcgt tttatgagcc accaagtcac tatcaattta ttcaatttta
    12181 ggaggtgagt ttttgattag tacagtatta tctttagaga gatataagaa gctacgtgtt
    12241 aaatatgcaa cacaaattga agatggaatg tttgatccga atggttttat agaatatatg
    12301 aaaccatttt ttgctgatcg cgaaagaaaa tatctagctt atactagcga aaagaatgaa
    12361 atagacaata gaccaaagcc taacactgat attgtaaaag ttaataataa acttcatgct
    12421 ggtatgtatt caattgtcgt agaccaagca gtcaatcatt ttactggtat acctatcaaa
    12481 tgggattacg atgtatcgga acaaaaaaga acactcattc aaagattaaa agataaattc
    12541 ttaaaaaatg atatagaact tccaacagtt ccagaagctt ttaacaaatt aacaagcaat
    12601 cttgattcca tgagattcgc aatgcttgat tctgaaactg caacttttca aggagcttgt
    12661 ggagtagctt tccgattgtt agagcctgtg gaagaagatg atggttggaa gttaagagca
    12721 agtaatattg aaccttggag agcagaaaga tatggaaatg ccggaatcta tatcaaagaa
    12781 aagtatgact cttaccagaa aaaatttttt caagaaatga aagttatgac aagaaataaa
    12841 attctgacat atgcttgcta tggtgatttg aattttgtta caagcggaac gttcaaaaaa
    12901 attgatgaga ccgataaccc tttggggacg attaccttgg cagaatttaa aaataatacg
    12961 aatcgttatt gtgattttga agtagctgag gaaattggag atgcaattga tcgtgctttg
    13021 tctgatcaac aaaacgaaat cgaacaattt aagcttgcgt acatgcttgt tactggcaca
    13081 acaatgagca aaggcactgc taaagaaatg atgaatcagc tagggattat caacttaaaa
    13141 gaccctactg caaaagctga atatgtcaca aaaaaattag caaaagattt caatgaatat
    13201 catatggatc tattgaaaaa acagttttac accatttgta aagcaatcga ttttaacgat
    13261 gaggtgttta aatcaaacag ttctggagaa gctcgcaagt ggcaaatcat tagcctagaa
    13321 gcgaaaacaa atactaaaga gcaatatttt agagaaggat tgaaagaatg cgcagaaaca
    13381 attgctgctt ttcttaagtt tcatgacaaa gtagaaattg agcctgaaaa aattattttt
    13441 actttctcta gatctttgcc aactgattta agttatttag ctgaagcttt acctaaactt
    13501 gcaccatacg tatccaaacg aactattcag agccaaattc catttgtaac agatgttgat
    13561 tatgaaaatg aaatgatgga attggaaagt ggaagtgctt atccagatag tgaatacaat
    13621 tttggcggag gtggcaatag tgacgataga aacgaagtat tggaccaaac gtcgagaact
    13681 ggaggatcaa gcaaggctca aacaagaaaa tcagacactt aaaaaattaa ctagtgtatt
    13741 tcctgaagca ctgaaagaga tacaagcaaa actattatca caagctgact tacacaatat
    13801 cacttatcca gaaatgatgg agttttacag tacaagtaat cagaaaaaat atcgtgaata
    13861 tgtggaaaaa aattataagt cattaaaaat gtatgatgca aaatacaaag agtttatcga
    13921 tgaatttttt ccaccatttg actatgcaaa agtcaatcgc ttattacaaa tacgatcaga
    13981 tgtatttaaa attcttgcag aatatgcgat ggatgcagat gtgaatcaat atttttctga
    14041 tcgcttagag gaaattcttc aaagaacata ttcttctaat gctaatgttt ttgttcaact
    14101 tttaaacgtt gatataccaa attatttacc agaaaatgaa attaaacatt atttgaacta
    14161 tccatggtca ggaaagacat tttcaagaag actttgggga aatatttcat cgcttgaaca
    14221 gaaactctct aatgctattg taaaaagtgt tgctagtgga gaaggcgtta tacacgcatt
    14281 aaacaccatg agattagact cagaaatttg tgacatgttt aagttagaag aatcaaagta
    14341 taacaaagcg atagaaaatc tcgttcgaac ggagtatgca aaatttgcac aagatggtat
    14401 tgaaaaatca tatttagaaa caggtattga ggaatacaac gtattgactg caaaagatga
    14461 gagagtttgc cggatttgtg gaggaaaggc aagtaagaat ccctataaac tgaaagatgc
    14521 tgtcataggt gaaaatcgag cacctttcca tagtcgttgt agatgtacgg atgttcctaa
    14581 tttaccaaaa ttaggaaagg atattgatga agaatatgac cgtttatttg gcgatttatt
    14641 agatgagttt gcacatgatt cttttggaat taatttgaaa cggaggaagt agaatgaaag
    14701 atttttttga agcagtacta acaattaatg taaatgctga tattgcagaa gcctacaaaa
    14761 cagctattga gtctgagaac catcctaatg gcttgagaga ccattggaat ggcaattatg
    14821 cctacgtggt tattggcgat caaactgtta attatcaaga taatactcca gttgataaga
    14881 ataccgttaa tttaacgatt caattattat ctcattcatt accaaattta aaagaaacag
    14941 ttgattggta tgaaaagatg ggatgtattg ttgttagaac tgactacaaa gaaggaaagt
    15001 ctagtaatta ggctttttta ttttgtccga aatgacgtta aactagcgca atactgggct
    15061 tggttgaatg gtggggcgca actattaaaa ctcaaagcaa tgcggggcgt gaaaacgaat
    15121 cgtggggcga aaggagaatg agcatgaaac acaaatcatt aatgccaatg aatttgcaat
    15181 actttgccga aggtgacgat caaaagtttt cttttgatga cttcaaatct tttgtggaat
    15241 ccaatgaaga agctcaaaaa tttattcagt cacaatctca aagtgttgcc gacaaacaat
    15301 tggaagcttg gaaacaaaat aacttagata aaattaaaca ggataccatc aaggaatatg
    15361 aggaatctaa gaaaaacaag tcacctgagc aaattcaact ggaaaaacta caagctgaat
    15421 ttgaagcaga aaaagcgttg cgtgtgacaa gtgataataa agcatttgtt gcagaacaaa
    15481 ttgctggatt agaactagat ggagagttaa aagagtctat ttctcaattt atgctaaata
    15541 atcttgttag ttcggataca gatttcacta agaatgctgt tgaaggtttt acaagtgttt
    15601 tgaatgcaat taatgagaaa catgcagatg cattaaaaga actacaaatg aagtctgcat
    15661 ttggtggaac tcaacaatcg aataaccagg ttcagcagaa caatgaaaca ttaacaaatc
    15721 cagaagaaca attaggacaa attcttcaac aatttaacta ggagagtgaa aaattatgaa
    15781 aaaaacatct ttaaataatt tagagtattt ggatatttca ccagcgatta atgctatgca
    15841 agtaccaaat acaccttttt taagctattt acttggtgct ggaaaaacag agccagcaaa
    15901 ctcgacagaa attaaatggc gtgaatatga tatcaacaac gatgattctt ctgaaaaact
    15961 tgagggcgga gaatatccag atgctgaatc aggtcgaact tggtttaaca actatactga
    16021 aatttttaga aaatcaacct ctgtatctgg tacattagat gctattaatg tgaatggagt
    16081 cggaaatgaa ttaactaatc aagtagcact acgtggtatg gaaatgaaaa ttgatttgaa
    16141 ccgaaaattg attactggtg taaaagctga tgaaaatggt tctaaaggtc gtcgaatgaa
    16201 tgggattttg aacttgatca attcagcaaa taaggcagaa acagccactg cgggtgcagt
    16261 aacaagaaaa gatatcgata ctttatttaa attgatgtat gaaaaaggtt atatgggaga
    16321 aaaactatgc ttgatttctc cagatatgca ggagttaatg actgatgagt tagatggaaa
    16381 atcaacaaaa attgttcagt tcggggaaag agtaactttt ggattgcaaa ttggaaatat
    16441 cgtgtctaat tacggtacag gtattgctct actagaacca tcattgccaa aaggaacaat
    16501 tgccgcaatc gatactaatt atgtgaaact acgtccatta cgtgaatgga gagcagaaga
    16561 acttgcaaaa acaacggatt ctagacgtat cggtcttgta ggtgaatact ctcttgaata
    16621 caatgcttca aactctgggg caattttaaa tttaaagtct gaataaaagg gagttagtac
    16681 tccctttttt gataggagga attacagtgg taaaaaaaga tgaaactaaa aaagatgaag
    16741 tcgtgaaata tagagtaggt aaaactaaaa attttgttgg gtttgttcat cctaaaactc
    16801 gtagatttat cacagcagat tcaaataacg aatttatcat ttctatagat gataaagaag
    16861 caattgcaat tttggaagat gcaattgatg ttaatgaaat ttaggaagtg atctgatgga
    16921 tgaatcgcta aaaacggaaa tcattgagtc tacaaaagaa gattttccag atttgagtga
    16981 agaacgcata actaatttat tagaaataat tttgctagaa attgaatcat acaacacttg
    17041 taaaaatgat atttcatggg aaaagttaaa aagcgtgatt agtgaagtgt tgtataaaat
    17101 aataaaaaat gaatcagaaa aaacagtatc ttcagttaga cgtggcgata caacgattag
    17161 ttatgcttca acaacaaatg atgttagtga gttacttcta ggctatggtg atttgatacg
    17221 aagagttatt ggttgtggag gattggagtt tttttaatga atgaagcaga tattttagaa
    17281 actacttatg aagatagttg tattattgaa agactgacgg acattgaaga ttctaataca
    17341 aatattacta ttcaagatta caaaaaagta tatgataatc ctatttcttg cgctctttca
    17401 caaggtcaaa tcgatggact agcagtcata gaagatggag agatggtaaa tgtttcaact
    17461 gacacatata aattatttgt tcatcctaag attaaactaa aaaaaggaga tcgaataaca
    17521 ataactcaaa aggccagtgg cttaattttt tctctatttg ctactaagcc tttttactat
    17581 cctagtcatt gtgaagtgaa tttgatagga agtgagaaaa atgggtaatc tcaaatatga
    17641 atcaaatgca gaaaagatca ttgagaattt taaaaatatg actgtaattg ctcaaaaaga
    17701 aggaatatcc ttcgttaacg attcaatgaa taaagtcgtt agtttagcta aaccattaac
    17761 tcctgtaaaa tcaggtaatt taaggcgagg atatcgtgta gtaaaagcta gaaaactatc
    17821 aagcggtcga attgttggag cagttataaa caatgaacat tattttaaat atgtagaaga
    17881 aggtcatagg actaaaaatg gtggctttgt caaagggaaa ttcatgttaa ctcgtgcaac
    17941 gaatcttgca aatatgtctt atattcctcg aagatttaaa caaatggcaa taaaaatcgt
    18001 taagaaagga aagtaacatg aaagatgaaa tcattgctgc aatcagtagc aaattaaaag
    18061 aaatctatcc tgatgggaca atatatcttg attcagttat gcagtcaact aaagattttt
    18121 atttcgtttt atcagtaatg gaatctggaa ctgaaaatgt aggaattgat attcaaaatg
    18181 tttctttctt aattgatatt gcattgattg ataataaacc taatagaaat ttaattaatg
    18241 aattagtctc acgttgtggg acttttttta atacgattac aatcgatgag caaatattat
    18301 ttccaaaagc ctatttacct gatgaagcag atggtgttca acatattcgt tttacattag
    18361 aatttccaca atatattgaa tggagtgaaa gataaatggg agaaaaaaga agtaaaaccg
    18421 gaattatttc tgtcgaaaaa cctacttggt ttcctttgga agatgagaca gggaattttc
    18481 cggtatatgg tactgcgttg ccaatgggaa cagcagtaag tatcaaacca acagctaatt
    18541 atgaaacaac acaagattat ggggattcag tggttcaaga tcaatttaca gcttttggtg
    18601 gtgcagaagt tgaactggaa gcaaatggat atactcataa agttttatct acaattactg
    18661 gagggaaaat tgttaaaggt ggtgccttgc gttctggaga agatattgcg caagatggtg
    18721 cgtttgcata tagacgtaaa aaatcgaatg ggaaataccg ttatacggtc ttttataaag
    18781 gacagtttgc tctagattca gatgaaacat caacgattga aggaagtaaa gtaagtttta
    18841 ctcatccaac ttggaagggt tcatttgtag atgtaccagg acttggatat atgtattcag
    18901 ttgatgaaga tgatgaaggc gtggataaag caatgatcgg aaattggttt acaaaagtag
    18961 caattccaat tgaagaagca gaactttcag gaggtacaga ataatgtcaa aatatcaaac
    19021 aacgattaaa ttaacgaaaa aagatgaaga aggtaaatat gagcaagtac aatttaaatc
    19081 tgctgaattt ctaccaggcc cagtcgtaga agaagcagca gaagtaatgg agggaatgca
    19141 aaatgctgta gatggaaaat cagtatcaga agcattaagt cgagcttatt catttattgc
    19201 agatattttg tttgaaggac agtttactgg tgaagattat cgtcaaggga tcgatgctcg
    19261 agaaattgct tcgttgacag gtaagttatt aaaatctgtt actgcaggct ttgatgaaac
    19321 ttatacagaa acgaaaaaaa agtaagcgaa gctctcaaat atcctttttt taaacattcg
    19381 attacttatc gagaattaga tttaaagacg caattactcg aagcaggttg gacgttacca
    19441 gaaattgaac gtactgactt tgacgagtta atgcgtcttt ttgcttttcg agatgcagta
    19501 aaagagcatg aggatgttga gtactacgat aacttcaccc aattttagga ggtgatactt
    19561 tgaataacga tgacctaatt ctgaagatga tactagatga atctggtttt actgccggta
    19621 tgaataatgc tgtcaaaaag ttgaactctt ttgatgaaac aatcgaaaga aaaagtagaa
    19681 atagtggcag ttcattaggt agtatttgga aaatatttgc tggaagtttt ctagctagcg
    19741 gagtaactag aatcgtagga gctggctttg atctaatcaa gggttccata agtggagcgg
    19801 ttgatcgagt agatacgatg aataacgctc tacgaaattt ccaaaatatg ggatttagta
    19861 attcagaaat tatgaagaat atcggaaaga atgggctttt atctcaaggt attcaaggac
    19921 ttcctactgc tttgaatgat gcgataagtc atgttcaact tcttgcttct tctacaggtg
    19981 atttaactcg ttcaactcaa atatttaaag ctttaaatga tggaattctt ggtttcggtg
    20041 gttcaactga tcaagttaat gaagctgtta ttcaattatc tcaaagtttc tcaaacggaa
    20101 aagtagacgc acaaacttgg aactcaatga tcaacgctca acttggacct actctttctg
    20161 ctattgctaa aaagatggga attacaatgg gagatctgaa agaaggttta tctcaaggta
    20221 agatttctgt tgaagaattc caaaatcaat taatagaaat ggataccaaa ggtggcggag
    20281 gacttaaatc attaagtcaa atcgctaaag attctactaa gggaattaaa acctctatac
    20341 aaaatgcaaa aacagctgtt acacgtggtg ttggtgaggt aatagaagga ttaaataaag
    20401 cattagtaga ttcagattta ggtggattta agggaattat tgataaagtg ggcagttcaa
    20461 tggaatcgtt cttaaaagta attgctgcaa atattcctac agcagtatca tttttaagta
    20521 atttatttaa cgaaattcaa aaatttggtt ctgctttgaa attcatgatg ccctttcttg
    20581 ttcctgcagc taccgctttt ggagcgttca tgtttcaact taaaggtata ccagcaataa
    20641 taaaaagctt caataatttt aagaatgcca taattggcgt tggaaattca ttaaagatta
    20701 tgggggcaat agctgctgct aattcatttg ttttaattat tggtgcagtc gttggtgcaa
    20761 tagctgtttt cggttacttt atggcaacca atgaagagct tagaaacaag gttatatcta
    20821 tttggaatga tgtgaaagat tccgtaattg gtgtattaaa aaatataaag gactggggaa
    20881 ttgatacttg gaattctgct aaataaatgg catcaaatgc agttgagggt gtcaaagatg
    20941 cttggtcagg aacaaaggaa tggttttcta acacctggaa aggtattaag gatggggcca
    21001 caggtttatt tgataaaaca gtagagactt caagaaatgc agtcgatagt gtaaaaaatg
    21061 catggtctaa tatgaagaaa tggttttctg atacttggca aagtataaaa gattcagcca
    21121 gagaaaaatg gaatggaatc aaaggttcta ttatgtcagt tgctggtcca ttgattacag
    21181 gtataaagaa tgcattttta catgttactt tttatctaga gaccttatgg aataatttag
    21241 ttgagattgg taaaaacgtc tttgagattt taaaaaatgt tatacttgcg ccggttttgt
    21301 ttattacctc catgatttct ggtggatggg aagaaacaaa aaataatatg attgctgttt
    21361 ggaataatat taaagaaagt gccataaata tttgggaatc tataaagaat atatttgtaa
    21421 gttattttac aaatatttat tttgctgcac tcaatatttg gacagggttc aagcttacat
    21481 tgataaatat ttggaatgaa gtggtaaatc aagctaaatc gatttggtta aacataaagt
    21541 atttttttat taatctttgg attgatataa a
//
LOCUS       DASJTC010000050        21167 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.050, whole genome shotgun sequence.
ACCESSION   DASJTC010000050 DASJTC010000000
VERSION     DASJTC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.050"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(280..1587)
                     /locus_tag="U4B19_001645"
     CDS             complement(280..1587)
                     /locus_tag="U4B19_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEN2077386.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1590..2171)
                     /locus_tag="U4B19_001646"
     CDS             complement(1590..2171)
                     /locus_tag="U4B19_001646"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEN2077387.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2287..2384)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2743..4035)
                     /locus_tag="U4B19_001647"
     CDS             complement(2743..4035)
                     /locus_tag="U4B19_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2077388.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(4032..4655)
                     /locus_tag="U4B19_001648"
     CDS             complement(4032..4655)
                     /locus_tag="U4B19_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077389.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4658..5494)
                     /locus_tag="U4B19_001649"
     CDS             complement(4658..5494)
                     /locus_tag="U4B19_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2077390.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5511..6398)
                     /locus_tag="U4B19_001650"
     CDS             complement(5511..6398)
                     /locus_tag="U4B19_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2077391.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6414..7697)
                     /locus_tag="U4B19_001651"
     CDS             complement(6414..7697)
                     /locus_tag="U4B19_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2077392.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(8380..9063)
                     /locus_tag="U4B19_001652"
     CDS             complement(8380..9063)
                     /locus_tag="U4B19_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEN2077393.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(9081..9698)
                     /locus_tag="U4B19_001653"
     CDS             complement(9081..9698)
                     /locus_tag="U4B19_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN2077394.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9708..10532)
                     /locus_tag="U4B19_001654"
     CDS             complement(9708..10532)
                     /locus_tag="U4B19_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEN2077395.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10525..11085)
                     /locus_tag="U4B19_001655"
     CDS             complement(10525..11085)
                     /locus_tag="U4B19_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077396.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11259..12215
                     /locus_tag="U4B19_001656"
     CDS             11259..12215
                     /locus_tag="U4B19_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2077397.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(12520..13476)
                     /locus_tag="U4B19_001657"
     CDS             complement(12520..13476)
                     /locus_tag="U4B19_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEN2077398.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            13580..14182
                     /locus_tag="U4B19_001658"
     CDS             13580..14182
                     /locus_tag="U4B19_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2077399.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(14270..15769)
                     /locus_tag="U4B19_001659"
     CDS             complement(14270..15769)
                     /locus_tag="U4B19_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEN2077400.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(15785..16309)
                     /locus_tag="U4B19_001660"
     CDS             complement(15785..16309)
                     /locus_tag="U4B19_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEN2077401.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            16607..17356
                     /locus_tag="U4B19_001661"
     CDS             16607..17356
                     /locus_tag="U4B19_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN2077402.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            17347..18132
                     /locus_tag="U4B19_001662"
     CDS             17347..18132
                     /locus_tag="U4B19_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEN2077403.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            18101..18817
                     /locus_tag="U4B19_001663"
     CDS             18101..18817
                     /locus_tag="U4B19_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2077404.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(18919..19953)
                     /locus_tag="U4B19_001664"
     CDS             complement(18919..19953)
                     /locus_tag="U4B19_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEN2077405.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(19957..21015)
                     /locus_tag="U4B19_001665"
     CDS             complement(19957..21015)
                     /locus_tag="U4B19_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEN2077406.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         6362 a         4492 c         3679 g         6634 t
ORIGIN      
        1 agttaggtca cattcctttt tatggataaa cggtgttctc ctgccaactc ctcggtcaca
       61 aggaacaata ttcaacaaac atgagaagct tttttttgaa attgttcctt gtgactcgga
      121 gatgaacggc aggctcacga cctcttcctt caacggtgtt caatcagctg accctcggta
      181 ttagctcaaa caaacggcaa aatacgagga actatttttc cgtttgtttc tctaacaatc
      241 aggtcaaaac gctgatttca caacctttct tttgctctat tacttcttga aggcttcttc
      301 caatatttct tcatcttttt tcaatccatt aaagatcaaa ttcaatatga tagccgtaat
      361 gctactcatt acgatgccat ttccagtgaa catttgcaat gtttgcggca tttgactgaa
      421 taatgtcggc gtaacattga aacccagccc aaatccaaca gaaacagcaa caatcaatag
      481 atttttatca ttgctgaaat caacttccaa cagcatacgg attccttgga ctgccaccat
      541 cccgaacatg actaacattc ctccaccaag taccggctca gggatgattt gtgctaacgc
      601 accgatttta gggaataatc ctaaaacaat caagaagaat gcagagaaat agatgggttt
      661 tcttgttttg attcccgaaa gttgaaccaa tccgacattt tgagaaaagc cagtataagg
      721 gaatgtattg aaaataccgc ctaagataac ggcaagtcct tctgcacgat atcccttttt
      781 caattcttct tctccaacat ttttcccagt aatgtcacca agtgcaaaat atacacctgt
      841 tgattctacc atgctgacca tggaaatgat gatcattagt aaaatcgacc atacgtcgaa
      901 agttggttta ccaaaataaa agggttgtgg aacatggaac aacggcgcat ctccaatcgc
      961 gctcagatca atcattccca tgaaagcagc taagatgctt cctccgacta aaccaatcaa
     1021 taccgcgatt gaacgaatga acccttttgc cagcatttgc acgcctacaa ttaggagaat
     1081 cgtcacaaat gcaagtagca gatttgtttt gtcaccaaaa tcagctgctg ttgcattacc
     1141 tccacccatt ttctcgattg ctacaggaat caaagtcaaa ccaataacgg tgataaccgt
     1201 tcctgttact ataggaggga atagtttttt aattttagaa aagacaccag aaactaacac
     1261 aacaaagatc ccagaagcga taatagatcc gtagatagct cctactcctt cattggttcc
     1321 gatcaagatc agtggagcta ctgcttggat cgcacatcct aaaacgacag gcaagccaat
     1381 tccgaaaaat cggttaactg tcaattgcaa taatgtcgct aatccacaca taaagatatc
     1441 aattgaaatc aaataagtca tctgttcttg attaaagcct agtcctgtac cgatcaaaag
     1501 aggcaccgct actgctcctg catacatcgc tagcagatgc tgcagcccta aaaccgcagc
     1561 ttttccattt tgtgtttctt tttccacgat tatgcgtcct cctcaagaaa ctcaacttgt
     1621 ccattcgtca aggaagcaat acgcgcaaga gaaacgactc tcaatcccat ttcttcaagt
     1681 aactgacgtc catcttggaa agacttttcg atgacgatcc cgattccttc tacttgcgct
     1741 ccagcttgct ggcataattc gatcaatcct tttgccgctt gtccgtttgc caagaaatcg
     1801 tcgataatca atactttgtc ttctgaagat aaaaatttac gagaaataga aatcgtactt
     1861 gtcacttgtt tcgtaaaaga ataaacagaa gctgtcaata attcttcatc catcgtcaaa
     1921 ctttttgctt ttctagcgaa gatcattgga acatctaatt ctcttgcagc aaataaagct
     1981 ggtgcgatcc cagaagcttc aatggtcaca actttcgtga ttccttgttc tttaaatact
     2041 tctgcaaacc gttttcccat ttgttccatc aatacagggt ctacttggtg agtgacgaag
     2101 ctgtcgactt ttaatacgcc ctcgcctaat atacgtccgt ctttttgaat acgttcaact
     2161 aattctttca ctttatttgc tcctttatca tttattaata ccgaaataac cgcttttgtg
     2221 caaaaaccta ctatgtttct gttaaagaaa aacaccctcc actttatcat aagaaaagaa
     2281 gggtgaaata gccaatcttt tacttatagt ccagcattta cggtactggc tagaaactgt
     2341 catccatatt atgacgatat ataagtaggt atgtaaaact aataatagtg taaattcatt
     2401 ttcttgcaac tgttttgcac gtgaaggcag tttgccatat ttgaataaaa aatgttgttt
     2461 caaatattga aaacagtcta acactacttt ctgaaactga caacgtggtt tacttatttt
     2521 tttgtaaaat ctaatgattt ttaatgcatt attccttttt gttcgggttt aaatagttat
     2581 ttttattttc tcctatctaa aaaagcagcc cacataaaaa gaaggcatga caaaaagcat
     2641 gtcatagccc ctttttccga ataaatagtg ttcaaaagac agcctatcgg caataaggca
     2701 agattttata aactacggga agtaatttcc gtaaatctat tcttattctt ttaagctgac
     2761 aagctcgtca atcactactg cttttcctga agcaatagaa tgatttgctg cgattcctgt
     2821 caaaatcgac aaaataccat ctacgtgagt tgctgcccga ttccacggat cttcttcttt
     2881 ttcttcacca aatagatcat ctaataaacg cggatctcct ccgccatgtc cgcccgcttc
     2941 ttgttcgacg attacctcat atggctcacc gaacatcgga tagacatgga tggactgaag
     3001 aacggctgct ccttcatcct ttttatctcc acctgcattg atgtaagact tttcgatcac
     3061 tttcatttcg atccgtcctt tgctgccatt gaaagaaaca ttgaatcctt cccacggcat
     3121 atacgcgttt agcgaatacg tcaaaatcgc accagtttca tatttgaccg ttacgcctag
     3181 tgtatcttca atagagatat catcggaaaa tacactgcgg tcacgaatat atccactgtc
     3241 tttttctgct tctaaataca tttctcgcaa ttcctcattg gctgacaggt ctatagcaaa
     3301 tggatcagct ttagctagtt cgcttccata gcatcgactg taaaatattt ctactccgcg
     3361 tttctccgca ttatgcatcc cataaaagtt taatccaccg aatgcaaata ccttttgggg
     3421 acggctgttc aaccaaaaat tcactagatc aaaatgatgg gtagatttat gaacaagaag
     3481 cccgccgcta ttgtgcttat ttcgatgcca tcttctgaaa taatctgccc cgtgttgcgt
     3541 atttaacagc cattcgaagt ggatagaaaa cacatcacca atcacatcac tttctatcag
     3601 ttcctttatc ttcgtgtgat acggtgcata acgataatta aaagtgactc gcaggcttct
     3661 tcccgtctgt ttttggaccg ttattatttc ttgagctttt tcctcatcga tagtcagagg
     3721 tttttcgcaa ataacatcat atcctttttt cattgcacga atgatataat catgatgcgt
     3781 tcgatctact gacgtaataa tgacttcagc cggccgtacg tcatccagta gttgctcaaa
     3841 ttgtgatggc atacatgtct ttactttttt ctgtccatac ttgttcacaa gtatccgatt
     3901 tgcataatcc atccttgttt gagatacatc acaaaaaccg actagttcac aagtttgatg
     3961 atacgtagag gcaattgcct cataaaaaaa acgcgctctc ccaccaagtc cgacttgtac
     4021 atattttttc ttcatttaaa gtcattctcc ttttctttta agcaaaatac tgtgacaaat
     4081 ggcagccctg gatacagtaa tgcagtgagt aaaaaatcat gctgtataca ccagaacagt
     4141 ccatacaaaa ggcataaata ccacagaaac cccttgacgt tttttgacaa cagccagata
     4201 ttccttttga tcaaataaac aagactatct gctttatctt ctttgcagat atcaattagc
     4261 gtactaaaaa aactatggga tacaaaaagc aacagacaca atagaaaagg aatcagatac
     4321 caagctccaa tctttcccac taataaatac caatcaactg caaaaataat gacaaacagc
     4381 tgaaacaacg taatgatgaa acacatccgt ttgatttttc ctttcctcca acataccttt
     4441 ttttctgcca agtaatggac agatagataa taagaaggat acatcatcaa aaaataaatc
     4501 agatagacaa cacgagcaga cagcactaat cctaaagtga acaaacatcc tagaactaaa
     4561 gcgaaaagag cattactgat aagactggtt aaaaaaatcc gacagacata gtagttgagc
     4621 ttgtaaagaa acatctcttc tattggcttt ttcatattca gcccttcaga ccgcttgtac
     4681 tgatcccctg gacaagatat ttattgaaga ataagaaaat cagaaaaacc ggtagcagcg
     4741 acaacgtaga catcgcaaac atactcgcgt aagtcgtact agatccagcg tctgcaaata
     4801 atttgatggc aatcgatacc gtgtaagatc tcggcttgtt taaataaatc aatggtccca
     4861 tgaagtcgtc ccatttccaa taaaactgga tgatgatcgt cgtgatgatc gaaggtttca
     4921 gcaaaggaaa aataattttg gtaaagacac tatacttgct gcatccgtca ataatcgctg
     4981 attcatctaa ctctttgggg atgctgacca tgaattgcat gatctggtaa atgaaaaatg
     5041 cttgtccaaa aaaatgtggt aaaatcagcg gcacatacgt cccaaccaaa ttcagccgat
     5101 tgtaaataat aaactgcgga atcaaaatca cttgtgcagg aatcatcatc gttactaaca
     5161 tgacgacgaa ccagaatctt tttcctttga aatcgattct cgataatgcg taagcaatac
     5221 aagtcgagct cagaactgtc cctatcgtcg aaactgcaga gatgacaaac gaattcctaa
     5281 agaaagtaaa aaatgaaatt ccagcaaacc ccgcccatcc ttttgcgtaa ttttcccatt
     5341 gaaaagtctc tggaatcaaa ttcagcgaac cactcataat ctcttgatcc gttttaaaag
     5401 aacctgcgat catccataag accggataaa tcatgactag acctaatagc aaaataaaac
     5461 cgtattgaaa ccatttcgtt actgtattat tcatttcttc ctcctctcat tcacacaacg
     5521 acccattttt ttgatgattt gaaaatcaga aaggtgacag ctcccataat caacagcatg
     5581 atccacgaaa tagcactagc ataccccata gaattaaaag aaaaagcttg gttatacaca
     5641 taaagagcat aaaagttcgt ggcatcattc ggaccacctt gggtaatgat aaatgcctgc
     5701 gtaaaagtca taaacgccgc gattgtctgc ataaccaagt tatacaaaat gatcggcgaa
     5761 agaagaggta acgtgatctt gtaaaacgtc tgccatttgt tcgctccatc gatctctgcc
     5821 gcttcataat acgagcctgg gatctctttg atacctgcag caaagatgat cattgccgaa
     5881 ccgaattgcc aagcagacat caaaacaaga gggatcatga ccagtttttg atcgctaaac
     5941 caggaaagcg caggtgcgcc taatcgttcc aggaaaatat tcactaaccc gtttttagaa
     6001 aacaactccc gccaaactag agcgatagaa atactgccac cgatcaaact aggcacataa
     6061 tacaaagaac gaaagaaagt cgttcctttg atattcctcg ttaaaagata agcaatcaat
     6121 agaccaaaga tcagtttcac aggtacagat gcaaatacat agagaaaagt gactcggatg
     6181 gagttcaaaa aacgggcatc actcagcatc tttatataat tatccaagcc gatccatgag
     6241 actggatttc ccaaacgata atcggtgaac gaataataaa gtgacaagat catcggtatc
     6301 actgttatca atagaaatcc taggatgaat ggagaagcaa atacgtaacc gaccacattg
     6361 tcttgatcca agaaccgttt tagtttttct tttttcatgc cttcctcctg ctgctatcct
     6421 agaatgtttg acgcaaactc ataaagactc tctgctgctt cttctgctgt tacttcttca
     6481 taaatgactt tttcaactaa aagattgtat tggtcttgaa tttccggatt caaagcacta
     6541 tcaaagacat tcacttcgcc gttgtccata tcggaaatca tttctaaata atcaaatgtc
     6601 gcttgcaaag ttgcatcatc tgcacgagcg agctgttctc gaatattcga catgattggt
     6661 acaccacgtt ctccttttaa aatttcattg gcttcttcac tgttttgaaa gaaatcaata
     6721 aacttagcag cttcttcttt atgggctgag ctttttgcca tactcagcat ttgcgaagaa
     6781 cgtaaaggga taccagattc tccatctgct tttctgcgag gtggattaat caactgaatc
     6841 tccatatctt caggagcccc tgacaatatt tctgtcaact ggttacttga tagaaacacc
     6901 attgccgctt ctctatccct aaccgcctga aaagacagat ccgtcgtact tgcagctcca
     6961 ccacctggtt ctggatgggc ccctttgtct accagatttt tacgcagctg gaaaaaatct
     7021 gttagatagt ctggattttc aaatcccaag ctgttagata gatcttcccg attataaaaa
     7081 ttcaatcctt tctcaacttg tggtatttga ataatacagc cagctataaa atcttcgaat
     7141 cttgaaatag catagattcc taattcttca gtgatttgat agctgatttg ttcaaattca
     7201 tcccatgtcc aatttgaagc tggttcaggg atatttaatt gttcaaatat atttttattg
     7261 taagcaatcg caaacgaatt caccccgtta gacagtccaa ctaattgccc gtcgtaagtc
     7321 gtcgttgcta aataggattc atctgcatta gaaatatcaa ttatcccttg ctccacatat
     7381 tcatctaatg gttcaatctg attgatgtat tgaggaaatt gattgttcag ttggaaaatg
     7441 tcccagatat catctgatgc tgctagtgta cctaattttg tgtaatatcc ttcataatca
     7501 taaaattcat aatcgatcgt cacatgggga tttttttctt cataaagctc gattactttt
     7561 actgttgcat cgtgtcggac ttgcgaaccc caccaagcca tgttcagcgt gatttttttg
     7621 tcatcttctt gcccacaagc cgtcaagaag aataacataa cacttaaagc taagatgatt
     7681 ctcattcttt tcttcattct ctgcacctct acttccattg ttatttttca atatactgta
     7741 tttttataca gaacatattt attaaacgat ctttttcaag gggaataaaa accaagactt
     7801 gccttatcgt tctgcctagg atgaacaact caatactttc aagaaaaatt agatagtgct
     7861 gattcttctt tcatcgctaa aaacacttct tgcttcaatg atgggaggat aattacgcaa
     7921 agaaaaaagg atatcagaca actgcccaat atccttgaca tagctaaata agcatgcttc
     7981 ttcgtgtgcg ctcttcccta aaaaacagaa aaataactag taattctgta tgaaattagg
     8041 tacaatatac acgtcgatgc acaagcattg actatggcaa cttgattggt cagttcccgc
     8101 tgacttttca agccccgaca atttagctgc tactaaattg tcgggtgcct tttttagttt
     8161 ttattcgttt ttcattctat agaaagcgct tcaataaatc aagacatttt tttgttagaa
     8221 tgctaataat aacaaaaggt tttaactctt cttatgtaca ttacaggtaa aaaaacaaaa
     8281 aaatttctct tttttttgaa ttctgaacaa atagtactgg agtacaaaag acgaggaaga
     8341 gccactatct gctcttcctc gtcttttgat tctcacctgt tattcatgat tctttttctc
     8401 agtttcccga tatttttcca ttttacgact aaaaagctct ccgctacttg gtggcgtata
     8461 tgtgatggca tttgctcctg cttgaatggt ctcagcaata ctttcttccg ttggaccacc
     8521 tgtcgcaatg ataggaagtt caggatattt tttacgaaaa tggcgtactg cttctgcagt
     8581 ttctttacct gcactgatat tgataatcga aataccagct gctaactttt catctatttc
     8641 tgtatatttc gacgttaccg tactgataac tggaatatca actactcgac aaacttcttt
     8701 tactgtctct aaaggcgtag gaccgttcaa aacgacccct aacgaacctt gcgcttctgc
     8761 gaacatgctc atgtaggaag aacgagcacc ttgtgtcaaa ccaccaccta caccggagaa
     8821 aactggaatg tcagctgcct caataatgct tttagtaatc gctggatgag gtgtaaaagg
     8881 atacacagca attactgcat tcgcatttgt attacgtatg atcgcaatat ccgtagtaaa
     8941 gatgatcgaa cggatcaatt ttccaaatat cttgatcccg cttgcttcta aaatgatttc
     9001 cggtactctt acgatatctt ttctcaattc cgtcatgatt tctggcgtaa attgttctgc
     9061 catcagccac ttctcctttg ctatctccgg tcatactgat tcaattgata cgtctcgatg
     9121 acctggatga ttttttgcgc ataaccgggg tctgtcgcat aacctgcatc ttgcaatgct
     9181 tgtgcggctt cctgatagtt ggtggccgta atcacttttt catacagcgc aggattccaa
     9241 tctactcctt ggacgaacag ttgtgtatgg tcatccatgg actgttccca tgaatcatac
     9301 actttgaagt ctccttggat agttatccat tcttcgttaa caaattcctt tgtttccagg
     9361 cttactttct tctgatcccc ataagccttg atcccgaaaa gattattgta tttgctagcc
     9421 aaggtacttt ttccccaatt tgattctaat atagcttgtc ctaaaatgat actaggaagc
     9481 actccataac catcttgtag ttcttgggca tgcggaacaa gccgttcaat aaattgctga
     9541 tgggattgcg cttcattttc ttgctgatta tcagtaaaag gagaagacag attcttcaag
     9601 gaaaaaacaa aggctaagag tatcactgct acaccggcaa ccactgctgg ccaacggata
     9661 ttcttgcgct tttgcttttt ccactttttt ttcgccatga tcaattccta cttttcattt
     9721 aattcattca gatattcttc tagcgtcaat tggtgtttgg tgatatactc agcgttttct
     9781 ttaccgacat aacgtaagtg ccaaggttca tatgtgatct tagttatatc ttcgtgtcct
     9841 tttggataac gaacgataaa tccatactgc gcagcattat ctgcgatcca tttagctcca
     9901 ggctgatctc cgtaacttgc atccaataca gtagaaggat aactgttgta ccagttttga
     9961 tcgacgacat cgactgccag acctgtgtgg tgttcgctgt atcctggctc cgtaatcgtt
    10021 tctttagtaa tctttgtcgc ctcctcttct gatacacctt gacgactcat cacatcttgg
    10081 acattttgtt cgaaaacttg ttgttgggat gatacagacc ggtaagctga gaccatcact
    10141 aatggaaatc cagctgcttc agcagccttg gcaaatgctt catattctgg agcaatgcgt
    10201 ttgtcgatca agtatccgtt acttaatgat gccaattgag tagactcatc aatttctttt
    10261 tctaatttgt gatctggacc aaccaaaact agcttccaat cgtctgtttt aacatctggc
    10321 aattgtttgg acgtacgatc ttttgtttct ttgtccttta ccttgctgct ggttgtttga
    10381 ctgttctttt ctttactagc agttgttttg gtgcttgttc gttcgactgc ttgattgccg
    10441 gcttgaacat gcgaatcatc atcgctaaaa ataacatagc ccattgctgc gatcataatc
    10501 acaactgctc caaatcctaa aaatttattc acttgaaaaa tcctttcgtt gttcttcacg
    10561 ttccccattc gcctcatgaa cgtaagtttc ttccagattt tcattcaccc aatcgagaag
    10621 ttcgattttt tctccttcga tctgtttttg aacatctgct ggcagacgaa aattcaaaat
    10681 atcatcatag ccccattcat catattgaat acttactttc aagtcaaggt atctaccagc
    10741 ctgctgatta tttttcaaag ggatcagctc gacttttgct gctttgctgc taagccattt
    10801 ttgcgcgatg cgtgttttga tttgcttgta ctctgcaacg gcaacgtttc gtttttcggg
    10861 aaaaataatc gtttgacgca aaatcttttg cgttagcatc cattcataat cagtgaaaag
    10921 attttttact ttctgcatcg cttcagatgg cagatttttc tcgccatctt tccatttttg
    10981 ccattgttct ggactgatca gcaagtattc atctaaaaaa tttttttcag tttcaaactg
    11041 ttctaataat acactcataa taagatcaat atatacttga tgcacgttta tcccctcctt
    11101 ttaactattc tacttaagtt ttgatcaaaa agcacctctt aaatcgctgc tttcattttt
    11161 tttaatgttt caagtcctct cctctttatt cttcgtgatt tttgttataa taatagacag
    11221 acaagaaact caatccaact atgaaggagc gttttcagat gaaaaaatat caatggggaa
    11281 tcgttggtct tggtaccatc gcacatgagt ttgccgaaag cttcaatcaa gagacgagcg
    11341 aactaacagc agttgcttcc agaacttcag aaaaagcgga aaacttcgct caccgttaca
    11401 atattccaaa agcttacgga agctatcaag aaatgttaga cgatgctgaa atcgacatcg
    11461 tgtacattgc tgtaccaaat agacagcata tcgaccatat cttggcagca ttggaagcag
    11521 gcaaacacgt tctatgtgaa aaagccatca cgatgaataa aaaagaactg gctgacgcta
    11581 tgcgcttagc agaagaaaag aatttgatct tagcagaagc gatgacaatc ttcaacatgc
    11641 cactttatca gcagttacgc tcaataatgg atacaggaaa actgggggct ttaaaaatga
    11701 tccaagcacc tttcggcagt tacaaagaac cagaccctaa aaaccggttc ttcaatccag
    11761 aattagctgg aggagctctg ttggatatcg gtacgtatgc tgtatctttc gctcgtttct
    11821 ttttgagctc tcaacctgaa gtagttgcct ctacaatggt tccttttgaa acaggcgtgg
    11881 acgaacaatc cgtcactatc ttgcgtaaca aagaaaatga acttgctgct gtcagcttga
    11941 cttttcaggc aaaaatgcca aaagtcggta tcgttgcctt tgagaacggt tatgtgacga
    12001 ttgctgatta cccgcgtgct gatcgtgcgg agatcttatt tacagatggt acaaaggaat
    12061 tcatcgaaag cgggaataca tctgaagcaa tgaattacga aataaagaat atggtacaca
    12121 tgattgaagg tgacttgccc aatcgctctc tcttcttgac aaaagatgtc atcgaaatct
    12181 tggatcaaat gcaagttcta tggaaaaata tgtaatttta aaaggttgtg aaaaagctgt
    12241 tctgcatcaa gtaataagaa cagccaaaca aaaatagctc ctcctatttt tcgtttggct
    12301 ggacttatta ccgcagagga aagcttttga acaccgttta agaaagaggt tgtgagccta
    12361 ccgttcagct ccgagtcaca aggagcaatt tcaaaaaaca gcttctcatg tttgttgaat
    12421 attgtgactt gtgaccgagg agttgacagg agaacaccgt ttaaagaaga ggttgtgaaa
    12481 tcagcttttt gacctgaata ttagctgaat tcacaacctc tatttttgga ttttttgttg
    12541 ggagaattct gccttgattt caataaaact tttaaaagac tgcaaaaact ggaacaattc
    12601 tgctcgatcc tcgaattcag ggcgactagt cggcagaggc atttgccgat acaatacata
    12661 aacttcttca atacgtgtta aaatttgtct gccatcattt gactctgaaa atgtttctgc
    12721 agtataaatc aataaaccat agatatgttt tccataataa gctggttgct ggattctctc
    12781 tagattttcc agcatgtctt taatcacatt ggcctgtgct cttctcatag agaagtaagt
    12841 ttcgtagtat agcggctgtc tgagccagta gttttcttga tgttctctgg catcttcttg
    12901 gctttcgcga atgaatgtca gtaaatgttc gcatttgatc tgcacatctt ctttattttc
    12961 tgataaaaga aattctgcca tttgtctcaa aatatttcga aattcttttt cgatctgtat
    13021 ttggttttcc ttcagctgct tcgtacgatc tggcatgtaa acatttgcaa gcaaagcaaa
    13081 tactaatcca atcgccatta atcccgcttc attgccgatc agctgccaag acatgttttc
    13141 ctccactaaa taatgagtga ccaaaaccga atttactact atcccttcgg ttaatttgaa
    13201 gcgagaggca agcgggatga acaggaataa aaaaagactg aagccaaaaa tagtataccc
    13261 aacagcaaaa aaactgaaca gtgctgttgc cgtagctaat ataaaggctg cgattcgatt
    13321 catgcccgta cgcaatgtag agcgcttggt atttcctaca cttaataatg caatgatccc
    13381 tgcagctggc caatacaaca gcgataagct acccgctgtg accatcgaaa agaagacact
    13441 gatcactgtt ttgactgttc gcattcctat acgcatttac ttcccccttt tttattttaa
    13501 ttatacttga ataatgaaaa actagctatc agacaaaagt ttaggtatga tagtaaaaag
    13561 accaaaaagg agaatctcta tgcaatttgg ccgttttcga ctaggtatgc gtacgatcaa
    13621 aagtgcctta gctgtttttt tatgtatctt gttttttcat ttgactcaac gaggaatccc
    13681 gatgattgct gcattatctg ctgttttttc tttgcgtcag gacttgacta caaccgtttc
    13741 ttttggaaag tctaggatca tcggcaatac attaggtggt acattagcta tcatttattt
    13801 ttttgtcaaa agctattttc acaatgattt tttggtagaa ttgatcttgc ttcctattct
    13861 agttattatt gtgatcgtcg tgtcagatgg tattaacaat aattccggta tcatttccgc
    13921 aatagctacg ttgttgctga ttgcgttgag catccctcaa ggcgaatccg ctttgtatgc
    13981 tatccaacgt gtactagata cttttatagg gacattcatt gcaatcggta tcaatttctt
    14041 tcttcgtcct cctgaaacag aaaagaagga agaactatca gaagatttgg tcgaactcca
    14101 aaagaaagaa cgattgctaa gaacaaattt acaagagata gaagctaaga tccaaaaaca
    14161 aaagaaaaat agcgaggact aattttcttt tgcaataaaa taaaaaaatg gagagaagaa
    14221 ctttaaaaag tcttcctcca tttttgattg aacggtgaag tgtactactt tattcttcct
    14281 tcatcgagtt ttgttctgcc tcttttggcg cttcatacat atttacttgg ctagtagtcc
    14341 cacgtgtcga caaattctct acgatttcct tcaagtcaag tcctgttgtc gcttttagtg
    14401 tttcttgagt agaagaaagc aaatttgtgg cataatttgt cactcggttc gctccactgg
    14461 tttctcctga actgcttcct gtatcgacaa cagagatttt ttcgatattt cctagcggct
    14521 gtgctgcttc gcgcatcaat tgcggtagca tatcgataac catactcaag acagctgctt
    14581 cgccatattc cttgaaggca ttggcgatct tttgttttgc ttcggcttct gcttttcctt
    14641 ttgctagtgc tgcttcggct tctgcttgtc cagctaaacg tgtcttattg gcttccgctt
    14701 ctgccagtgc ttccacacgg aatcgttctg cttcggcttc agtcacttct ttgacttttt
    14761 gagcttgtgc ttcttgttct ttagcgtacc gatcggcatc tgcttttttc ttcacttccg
    14821 aatcatattg cttttcacga cgtgtgattt ctttttcttc cagttcgatt tgtttttgac
    14881 gttctacgat tttaatctgc atttcctgtt cgattacttg ttgttgtgct cgggcacttt
    14941 ctaaattgta ggcttggtct gcttttgctt tagcaacatc ttgttcttgt ttgtaggttg
    15001 ctaatttcag ttctttttct tttaatgatt ctgcgatctc tgtttgtcgc tgtaattcag
    15061 ctgcttgtga ttctttttct gcttgagctt ttttgatacg cgtttctttt agagcttccg
    15121 cttctgctat atctgcatct cttttgactt gggcgattct tggtttccct aaagagtcaa
    15181 gataaccgtt tttgtctctg acctctttga tagtgaaaga aacgatgatc aaccccattt
    15241 ttgcaaggtc tacagaagcg acttcttgta cgctttgact gaacttatcg cgattttgat
    15301 agatttcttc tacagtcata gaaccaagaa tcgagcgcaa gtgtccttct aatacttctc
    15361 ttgcttcatt ttccagttct tctcttgtct tgcctaaaaa ctgttctgct gctgtagcaa
    15421 tctcttctac agatgaaccg attttgatga tcgaagtacc atcacacatc accggtacgc
    15481 cttgttccgt atacacttca ggggtggaga cgtccaattt actcgataat agactgatcc
    15541 gattcgaacg ttgaaaaact ggcaagacga atgcgccacc tccacgcaca attttgattt
    15601 tgttattgct ttcatcaatg tgcacatttt tgcttcctaa ataacttcca ctgatgatca
    15661 acgcttcatc cggttttgct gtctggtact tcgctacaaa gacgatcagc aacatcagta
    15721 aaatcaaagc tgcgattgcg atcgcgataa aaacaactgg tatttccatt taacacacgc
    15781 tcctttaagc ttttatcatg ctttcataag gcatgacata acagattctt tccttgatct
    15841 caatgatcaa tactttcgtt ccccgctcag ctgtctgttc ttgggggctg taaaaacaag
    15901 ctggacgact aatggaaccg gtaacacttt tcaactgaat ttctcccata cctcgtacag
    15961 ggatcggtgt gatcacaaca gctacttgtc cttccagagt tttttcagaa gacgacatcg
    16021 ttgattcggc atgtttcatc ggcatcaata tatagaaatt aagcaaaaat accaacacta
    16081 tcgatatgcc tccactagca ataaataaaa ataaagatga ttgatctgtt aaacgctcac
    16141 ctagaaaacc aaataagctt gtgaaagtta accaagggat aataagcatc ggatcaagcg
    16201 ggccatcaaa atcgaataca tctccaaaaa taatcaatag gactgccaat cctgcacaaa
    16261 tcagcaatgt ataccaataa atcgtctcaa gtgcgattcc ccctatcata tacacgcctc
    16321 cttccatttc tacgtctatc ataccaatgt ttgaaaattc tgtatagctt cttttaccga
    16381 ctcagtggtg ttttttatag gaaggttgtg tttatcgttg acaatttgtt aaatttttgt
    16441 tttcatgtta aaattttcac aataaattct ttcgaggtag cctatgtttc ctttttttaa
    16501 gaaaaaacca ttaaagctcc agaacacgat catccttgtc gtttgtttct ccacattcgt
    16561 ttctttgatt gtttctgcca tattgatcag gaattttatt ctttcgatga accgatctag
    16621 tgtgtcagat aaaatctcta gcattgctaa aatcgttgcc aatgataaac tcatccaaaa
    16681 aaagctgcag actggtgacc cgacacatgc gattcaagga ataacaaatg aaatcatgat
    16741 tgcatcgaat ttagatttta tcgttgtcat ggaccctgaa ggcattcgat attctcacgc
    16801 agacccaagt tacatcggtc ttcctttctc aagtcatgaa gatgtgaaaa aagtgattgg
    16861 aggacaagaa tttttctctg aaaaagaagg aatgttaggg ttcggcttcc gctatttcgt
    16921 ccctatttat tatgatcaag agattattgg tgcagtttgt gttggcttaa caaacaacac
    16981 gattgacaat caagtcaaag atggacaaaa aatgattgtt ttttcattga ttattggact
    17041 tagcgtaggc atcataggcg ccgtttttct tgcccaaaaa atgaaaaaag tactgcttgg
    17101 acttgaacca caagaaatag cagaacagtt aagtgaaaaa acgattatcg aaaatgaagt
    17161 agatgaagga atcttagcca tctcttctga taaaactatc ctcttgatca atacacctgc
    17221 aaaaaacatt ttctaccaaa cagagcagct attggatttc gaagtaggaa aaaaatcagt
    17281 gataatctat atgacgtttt gtttaaagag acgattgagc atcaaacgac cgttcggact
    17341 caatccatgt atttaaatgg gatagaaact atttctagcg ttacacctct ttttagcaat
    17401 gaccgttttt taggagcagt cgcaacttta cgaaatcgat cagaagtaat gaaattaatg
    17461 gaggagcttg gggggacgaa acaatatata aactccatga gagctcaaac ccatgaattc
    17521 atgaataaac ttcatgtcat ttctggtttg attgatttga agaagtatga agaagtcacc
    17581 cattttatcc agcagctaaa tcaaaattat aaaaaagaga ttggccaagt ctcaagtctg
    17641 atcaaagtac ctgcaattgc cggtttttta ttaggcaaaa taaacgaagc caaagaacaa
    17701 tctgttgaaa tgctgttaga aagcgattct tttatcccta aaatggagat ggatgactcc
    17761 atccattcac ttttgcaagt gttagggaac ttacttgata atgcaaaaga agccgtgcta
    17821 caaaaacatg aaagaaacgg acaggtccgg attcatttaa actatgacac agaagctgaa
    17881 gtattcatcg tgaccgtctg cgatgacggt gtcgggatat cagaagaaat ctcagaaaaa
    17941 atatttacgc taggtttttc gacgaaggga atcaatcgtg ggtttggatt gaatctcatc
    18001 cattcaatcg tcaaacgcca cggtggattt ttagaatttt ccaaacaaac aaatggcggt
    18061 acaatcgtct atctagaatt accaaaaaag gaagatcaaa atgatgacaa atgtcctgat
    18121 aatcgaagat gatccaatgg ttgcagaatt gaatcgtact tttgtggaaa gcatccctaa
    18181 tatgaacgta atcgaaagtt tcagaaatgt agaagaagga aaaaaatatt tcagacaaaa
    18241 aaacgcagat ctgctgctgc ttgatgtata catgcctggc gaaagtggta tccatttttt
    18301 atcttggctt agacagcaag gaaatgatat cccagtgatt ttgatcactg ctgcagatga
    18361 tatcgaaacc ataaagaatg tgatccgcta cggtgcctta gattatttga tcaaaccgtt
    18421 cactttgcaa cgttttcagc tggcgatcga aaagtatctt gctttctctc agacaatcca
    18481 aacgactgaa aaagccgatc agaaattgat cgatcaagta ttcaaccaca ccactcccat
    18541 tcacgaaata ccatcattca tcgatttgcc taaaggtctg tcaaaactga ctttaaaaaa
    18601 aataagtgca tttattctaa aagaagatac tattttttca acaaaagaac tggctcaaaa
    18661 aagctcactg tcacgtatat ccgtcaagaa atatttgaat tttttagtag aacaagatat
    18721 attgaacgaa agaatcaaat atttggaaat cggccggccg ctaacctgtt atgaattgac
    18781 aaagaatggg cggaaaaagc tcacagatta ctgttgagtc atcgaattat cccatacaaa
    18841 atagtccaag tatacagaag taacaactgt ttacttggac tatttttaaa cattttattg
    18901 acgaatatcc atgttttctt atcttaattg acgggcttta ggaagagata cctcattttt
    18961 cgtttttcca gtttctatta tttcacgaaa gttctcgaaa cttgtcgaaa tcatatttga
    19021 taatgcctca tctgtatttg aacctacatg cggggttaca agaactctag ggtacatttc
    19081 tactaattgc tggatagcag ggtctggaat cgtctcccat ggcttgaagg atcggaaaaa
    19141 aacctctgtt tcatttgcaa aaacatcggt tgcaaaacca gctaaatgat tgcttttcaa
    19201 agctcttaaa atagcttcat tatcttgaag ctctccacgg gctgtattaa tcaagatgct
    19261 accttttttc atttgagcta aaaatccttc attgatcatt tgatcgtttt cacctgggat
    19321 atgaggtaca tgaatactga caatatcgct tttggctaat aactcttcaa gcgagtggaa
    19381 cgtcaatact tttttagcag cttctgtctg atgaatatcg tagccgatca ctttggctcc
    19441 tagtccctta aacaaaacag cttccgtcaa ccctatcctg cctgttccta tgatccctac
    19501 tgtgcaattt ctgatttcac gactaaacat ttgctcatcc actcgaaaat ctttgaaaga
    19561 tgttcggatc gtcgtatacg cagtatttct caaaagcatc atggcaaaag tcaaagataa
    19621 ctctgctatc gcatttggcg aatacgatgg tacacgggct actgtcatac caaaatcagc
    19681 tgccgcttgt agatcgatat gattgactcc cacagtccgt gtaaaaacat aacgaatccc
    19741 atattcctgc atttttgcaa gattggtctc atctgctata caatttcccc tcaacaatac
    19801 agcatcatgg tcctttgctg acacgatatt gtcatgtgtc aacagttctt cataaagact
    19861 gagatcataa tggtaaatat tcaatttttc aaaaaaatct atctcgttcg gacgaacacc
    19921 gtaacatgcg attctcatac tttcttcctc catttcttat gaaaataaac ctatgccatg
    19981 aatcatttgt aatacaacga tccatacagg tgtcagtacc acagcaaaaa tagttgacag
    20041 taaggatgca ttggaggcta aaagtgcctc cctatcaaaa ctgatagcat aggctgctgc
    20101 cactgttgca gctggtgtag ccatcatgat aacgattgtt gccagtgctt caaaattgac
    20161 tggtagaata ttcgttgccg tcaaaactgc tagaatcacg atattcaatg caggtacaaa
    20221 taccactttg actagactgt aataccatga agttttttca gatgcagctt ttttgaagct
    20281 tacttcacct aatgttgcgc cgattgctag ccacgccaat ggtgaactta aaccagctaa
    20341 ataatccatc ggctgaaata gccacggtgc agtctggtca attcgcaaaa acgctacttg
    20401 gcttatctct ccttctgcac ctgtaactga cacttgtggc agataccctt ggaacagcca
    20461 aatgaaaagg ccggcaaaag tagcgataat gattggattg acgaacatcg ctttgatatt
    20521 tttagcttcc atcttcaagc cactcatttt tatgtatcca taagaataca aaaagattcg
    20581 gtagccaata ttgaaaattg aggaatacat cacgccgaca ggaccataaa tcgcactgac
    20641 gataggcgtt ccgaaaaagg tcgtagaacc aaagatcgtc aggacacgca acgttgtttg
    20701 cttatcccct ttatagctta agaaaaaagg ttttgaaact agaataagaa taatataaat
    20761 aactaatccc caaatcaaga cattgattcc ttgcatcagt aattctgtat tgatatcttg
    20821 catgaaagct cgaaatgcca gtgctggcaa agccactgtt aagacgactt tcgatagaat
    20881 ttttccaaca gattcagaaa aaatcccctt ttttctacaa acaaacccta ataatataat
    20941 aaaaactgtt gaagtgattg cactgacaat cgacatatct gtcaaagtcg atcgaaaaat
    21001 gtcaaataag ttcatctttc tcctcctaat tatgttcatt tattcacaat aaaaaaagct
    21061 tttattttgc taatacgtat taacattttt aattttatct ttacattgtt acttattgaa
    21121 caataggaaa ttaaagaaac ttttgaaact tatgtaaagt gattttt
//
LOCUS       DASJTC010000051        20967 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.051, whole genome shotgun sequence.
ACCESSION   DASJTC010000051 DASJTC010000000
VERSION     DASJTC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.051"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            289..606
                     /locus_tag="U4B19_001666"
     CDS             289..606
                     /locus_tag="U4B19_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN2077407.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="U4B19_001667"
     CDS             complement(697..1062)
                     /locus_tag="U4B19_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077408.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="U4B19_001668"
     CDS             1270..1752
                     /locus_tag="U4B19_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077409.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /locus_tag="U4B19_001669"
     CDS             1949..2839
                     /locus_tag="U4B19_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEN2077410.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3165..5639
                     /locus_tag="U4B19_001670"
     CDS             3165..5639
                     /locus_tag="U4B19_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2077411.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5742..7478)
                     /locus_tag="U4B19_001671"
     CDS             complement(5742..7478)
                     /locus_tag="U4B19_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEN2077412.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7475..8179)
                     /locus_tag="U4B19_001672"
     CDS             complement(7475..8179)
                     /locus_tag="U4B19_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2077413.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8310..8813
                     /locus_tag="U4B19_001673"
     CDS             8310..8813
                     /locus_tag="U4B19_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEN2077414.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8773..9111
                     /locus_tag="U4B19_001674"
     CDS             8773..9111
                     /locus_tag="U4B19_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEN2077415.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9132..10550
                     /gene="ffh"
                     /locus_tag="U4B19_001675"
     CDS             9132..10550
                     /gene="ffh"
                     /locus_tag="U4B19_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEN2077416.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10662..11240
                     /locus_tag="U4B19_001676"
     CDS             10662..11240
                     /locus_tag="U4B19_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEN2077417.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11244..11765
                     /locus_tag="U4B19_001677"
     CDS             11244..11765
                     /locus_tag="U4B19_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2077418.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11844..12398)
                     /locus_tag="U4B19_001678"
     CDS             complement(11844..12398)
                     /locus_tag="U4B19_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN2077419.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12651..12926
                     /gene="rpsP"
                     /locus_tag="U4B19_001679"
     CDS             12651..12926
                     /gene="rpsP"
                     /locus_tag="U4B19_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEN2077420.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12938..13189
                     /locus_tag="U4B19_001680"
     CDS             12938..13189
                     /locus_tag="U4B19_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEN2077421.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13313..14104
                     /locus_tag="U4B19_001681"
     CDS             13313..14104
                     /locus_tag="U4B19_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077422.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14274..14795
                     /gene="rimM"
                     /locus_tag="U4B19_001682"
     CDS             14274..14795
                     /gene="rimM"
                     /locus_tag="U4B19_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEN2077423.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14785..15546
                     /gene="trmD"
                     /locus_tag="U4B19_001683"
     CDS             14785..15546
                     /gene="trmD"
                     /locus_tag="U4B19_001683"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEN2077424.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15682..16029
                     /gene="rplS"
                     /locus_tag="U4B19_001684"
     CDS             15682..16029
                     /gene="rplS"
                     /locus_tag="U4B19_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEN2077425.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16339..16581
                     /locus_tag="U4B19_001685"
     CDS             16339..16581
                     /locus_tag="U4B19_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEN2077426.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            16932..17186
                     /locus_tag="U4B19_001686"
     CDS             16932..17186
                     /locus_tag="U4B19_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HEN2077427.1"
                     /translation="MLELSKQLPVSDPRHFDYEEIAIKILEELQKNYTTKRVNGSNGL
                     LLHAVYDKNSLKGVDECVIWGDYFYVEGITRLAKTWYCYW"
     gene            17215..18474
                     /locus_tag="U4B19_001687"
     CDS             17215..18474
                     /locus_tag="U4B19_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEN2077428.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18527..19423
                     /locus_tag="U4B19_001688"
     CDS             18527..19423
                     /locus_tag="U4B19_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2077429.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19434..20303
                     /locus_tag="U4B19_001689"
     CDS             19434..20303
                     /locus_tag="U4B19_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2077430.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20313..20967
                     /locus_tag="U4B19_001690"
                     /pseudo
     CDS             20313..20967
                     /locus_tag="U4B19_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
BASE COUNT         6918 a         3608 c         4286 g         6155 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2941 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     3001 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     3061 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     3121 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     3181 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3241 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3301 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3361 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3421 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3481 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3541 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3601 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3661 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3721 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3781 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3841 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3901 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3961 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     4021 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     4081 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     4141 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     4201 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4261 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4321 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4381 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4441 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4501 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4561 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4621 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4681 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4741 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4801 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4861 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4921 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4981 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     5041 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     5101 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     5161 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     5221 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5281 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5341 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5401 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5461 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5521 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5581 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5641 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5701 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5761 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5821 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5881 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5941 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     6001 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     6061 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     6121 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     6181 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6241 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6301 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6361 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6421 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6481 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6541 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6601 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6661 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6721 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6781 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6841 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6901 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6961 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     7021 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     7081 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     7141 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     7201 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7261 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7321 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7381 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7441 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7501 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7561 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7621 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7681 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7741 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7801 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7861 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7921 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7981 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     8041 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     8101 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     8161 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     8221 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8281 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8341 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8401 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8461 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     8521 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     8581 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     8641 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8701 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8761 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8821 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8881 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8941 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     9001 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     9061 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     9121 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     9181 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     9241 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     9301 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     9361 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     9421 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     9481 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     9541 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     9601 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9661 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9721 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9781 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9841 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9901 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9961 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
    10021 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
    10081 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
    10141 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
    10201 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
    10261 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
    10321 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
    10381 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
    10441 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
    10501 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
    10561 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
    10621 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10681 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10741 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10801 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
    10861 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10921 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10981 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    11041 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    11101 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    11161 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    11221 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    11281 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    11341 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    11401 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    11461 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    11521 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    11581 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    11641 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11701 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11761 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11821 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11881 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11941 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    12001 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    12061 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    12121 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    12181 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    12241 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    12301 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    12361 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    12421 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    12481 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    12541 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    12601 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12661 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12721 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12781 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12841 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12901 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12961 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    13021 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    13081 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    13141 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    13201 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    13261 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13321 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13381 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13441 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13501 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13561 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13621 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13681 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    13741 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    13801 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    13861 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    13921 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    13981 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    14041 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    14101 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    14161 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    14221 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14281 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14341 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    14401 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14461 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14521 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14581 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14641 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    14701 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    14761 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    14821 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    14881 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    14941 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    15001 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    15061 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    15121 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    15181 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15241 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15301 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15361 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15421 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15481 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15541 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    15601 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    15661 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    15721 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    15781 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    15841 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    15901 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    15961 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    16021 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    16081 cttagcatgt aatttgaaca ccctgatcca gtttttttct ttaattattg gtaggggaga
    16141 ataatggatt agaataaatc tacctataag tagtagtaaa aaaaagagca ctatgataat
    16201 atctttctta agagtaaccg ctttaataaa aaatccagaa tattctaaca taaatggagt
    16261 attcttagaa tcaaaaggag aaagaagatg gtttatactt atacggagca agaaaaaatg
    16321 aaagtttgag acacattcat gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa
    16381 aagaaaaaca tcctattgaa agaagtcttg aaacgctttg accatggagt gacttataca
    16441 gaaacagagg ttaattcgat actgctgaac gtattttcta gtggcgatta tgtcgaacaa
    16501 agacgttatc taatcacatt tggctttttc aaaagaagtt cagacggtcg ggcatatcag
    16561 atgatgggaa ttgaaaatta gaagttttaa tttatactca ttgaaaaaag ttcaagtatg
    16621 cgatattggt tctacaccgc atacttgaac tttttttgtc atctgtatct ataatacaaa
    16681 gccaagaacg ttattcaaaa tatggatagc tatgataaaa gctaatgagc gattcgcagt
    16741 taaataagca atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat caattggatg
    16801 cgttacatga ataaagataa ttctctttta tttgtacgaa taaataccaa aacgtacctc
    16861 ctaaaattag attcaaagaa gatagcaaag aggaaaaaga tagttctgca gcagctatag
    16921 cggcatgtgg attgctagaa ctttccaaac aactgcctgt ttcagatcct cgtcatttcg
    16981 attacgaaga gatagcaatc aaaatcttgg aagaattaca gaaaaactat acaacgaaac
    17041 gagttaacgg atcaaacggt ctgcttctcc atgccgttta tgataaaaac agcttaaaag
    17101 gtgtagatga atgtgtgatc tggggcgatt acttttatgt agaaggaatc actcgtctag
    17161 ctaaaacatg gtactgttac tggtgatgta taagagaaga ggaggattca tataatgaag
    17221 cgatggaaaa aaatgatgat tggatcaata gttttaggaa tgtcactcgt atttggtgct
    17281 tgtggtaatc aagaagacag tagtgaagaa tcagcaaatg aaattaccgt ttcgacatgg
    17341 aattatgaaa caacacctga attcaaagcg ctctttgaag cattcgaaaa gaaaacggga
    17401 atcaaagtga aagcagtaga tattgcttca gatgattatg atacgaaact caccacgatg
    17461 ctttcttctg gagacacgac agatgtattg acgatgaaaa atcttttgtc gtattcaaat
    17521 tatgctttac gagatcagtt agtcgatcaa acagaacgaa ttagagaatt agacactgat
    17581 gctgctgaag aaacatatga aatgtatgat atcgatggaa aaacatatgc attgccatat
    17641 cgaacggact tttgggtact atattacaat aagaaaatgt ttgatgatgc aggaatcgaa
    17701 tatccaaaaa atctaacttg ggacgaatat gaagatttag caaaaaaact tagtaaaaat
    17761 gacggacaag tttacggagc atatcaacat atctggcgtt cgacaatcca agctattgca
    17821 gctgctcaaa acgaggcgaa tcttgttgaa ccagattatc aatatatgac tgattattat
    17881 gatcgagcat tgaggatgca aaaagaaggc gcgcagatgg attttggcac ggctaaatca
    17941 acgaaagtaa cctatcagtc tcaatttgaa gaacaaaaag cagcaatgat gtacatggga
    18001 acttggtata tggctggcat actcgcaaac aaagaagcaa gtaagacaga agtagaatgg
    18061 ggaattacag ctattcctca gaagaaaaaa ggagaatcgg tcacgacgtt tggctcacca
    18121 acagcatttg ctgtcaataa aaactcaaag aaacaaaaag cggcgcaaga atttattgaa
    18181 tttgcagcca gtgaagaagg agcaaaagta ttagcaggag taggagtagt tccttcatat
    18241 cgtacggatg agattgatca gctttatttt aatctcgctg gaatgccaac ggatgaaatt
    18301 tctaaaaaag ctttttctcc tgatgaaatc aaacttgaat ttccgattga tacgtatggg
    18361 cctgcaatcg ataagatttt acaagaagaa tatgacttag ttttggttgg agatgaaacg
    18421 ccggaaaaag gaacggtaaa tatggagaaa cgtgtagctg cggaaaaaga ataatcatga
    18481 aaggagagaa gccaagagat aaaacactct tggtttcgtc atcgaaatgg agaatagtat
    18541 aaataagaaa gctacaaatc atttgcgtag aaaacgaacg tggatcgctt attcgtttat
    18601 cgccccgaat ttcattggtt ttttcgtttt tacattaata ccaatcctgt tttcgcttgt
    18661 actagcattt atgaaatggg attctttttc aacgccagaa tttgctggac tagataactt
    18721 tcgtaaaatg gccaacgatg agacattttg gatttctttg aaaaatactt ttttatatac
    18781 cgctgctgta gtgcctggca cacttgtttg ttcgttaggg ctagctttac tactaaatca
    18841 gaaaattaga gggatgaagc tttttcgtac gatatttttc tttccgtatg ttacttcctt
    18901 agtagcaatt gctgttgttt ggaatatgtt gtttcatcca acagtgggac ctattaatat
    18961 ctttttaaga cgatttattg aacatcctcc aggttggact tcaagttcaa attgggcgct
    19021 ttgggcaatt gtaattgtca gtatttggcg agggatgggt tattacatga ttctatacct
    19081 tgcaggacta caaagtatcc aaaaagaact atatgaagcg tcttcgctag atggagcaaa
    19141 ccgttggaaa caatttttga atgtgacatt accttcgttg agaccaacaa ccttctttgt
    19201 aacgatcatg cttgtcatca attgtttcaa ggtgtttgac cttgtgcaag tgatgacgga
    19261 tggcggccct ggtcgagcga caaatgtgct ggtttaccaa atctacaatg aagcattcaa
    19321 aaaatttgat tttggttatg cttcagctat ttcacttgtt ttgttcgtaa ttgttttggg
    19381 cgttacggtt gttcaatttt cttggaataa gcgtcgtact taggaggtgt gatatggaag
    19441 agagtgttcg aaatgaaaga aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc
    19501 aatggttggg tatgattctg ctgattgtat tagctttatt gacgatcatt ccttttttat
    19561 ggatggtttc tgcttcattc aaagcaaata atgaagtgtt aacgattccg attcaatgga
    19621 tccctaaaac ttggcattta gagaattatc tggttatttg gtctcgtatt cctttagtga
    19681 cattttttaa gaatacagct tttttaagcg ttgtgatcac gttgattcag ttgtttactt
    19741 ctagttttgc tgtttacggg ttttctgaac taaattttaa agggcgcgat attttatttt
    19801 tagcatatat cggcacgatt gctgtccctt ggcagtctta tatgatcccg cagttcatta
    19861 tgatgagaaa agtgggttta acagatacgt tgtggtcgtt ggtgctatta caagcgttca
    19921 atgcttttgg tgtgttcttg ttgaagcaat attacagtag tatcccagat tctttatgtg
    19981 agagtgcgcg gatcgatggc ttatcagagt gggaaatcta tcgcaagatc attttacctc
    20041 ttacaaaacc ggcgattgcc agtttgacga tcatcacatt cgttaataca tggaacgatt
    20101 atatggggcc attcatttat ttgtcttcta cggaaaataa aacaatccag ttagggttga
    20161 aaatgtttat tgggctattt gatgcagagt acgcattaat catggctgcc tcagttgttt
    20221 ctgttcttcc tgttgctttt atatttttag cgatgcaaaa atattttgta gaagggatcg
    20281 cttcatcagg aatgaaggaa taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg
    20341 tttatatgga attcttttat tggttgtatc aatcattggc aagtagcctt tgtttttggt
    20401 gtgtcaatat cttgtatata gcagctgcgt tccttttgtc gatagatgga aggaatgtct
    20461 tttcctttat tttttcttta ctttttattg gtccaggttg catcgtgatg ataggtatga
    20521 cagaagaatt agcaaaagaa aaaagttttt caccagtacg tttgttcata aagacgtttc
    20581 gccaattttg gctaaaggga tttttttatt ggctatttgc ttggatagtt agtgtgataa
    20641 tgatttttga ttgtttcttt tttatacgtt tttcttatgg aaaatggttg attccgttgt
    20701 ttgttttact tgcttgtttt acttgcttgt ttatcagttt cctttagcat caactgctgg
    20761 tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat agcgttctac
    20821 tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac aagtttgttt
    20881 ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat cgtatttggt
    20941 ttgatttact tcaacaatcg taaattg
//
LOCUS       DASJTC010000052        20799 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.052, whole genome shotgun sequence.
ACCESSION   DASJTC010000052 DASJTC010000000
VERSION     DASJTC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.052"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="U4B19_001691"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="U4B19_001691"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEN2077431.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="U4B19_001692"
     CDS             complement(2260..2511)
                     /locus_tag="U4B19_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEN2077432.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="U4B19_001693"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="U4B19_001693"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEN2077433.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="U4B19_001694"
     CDS             3461..3679
                     /locus_tag="U4B19_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077434.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="U4B19_001695"
     CDS             complement(3968..4480)
                     /locus_tag="U4B19_001695"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEN2077435.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="U4B19_001696"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="U4B19_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEN2077436.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="U4B19_001697"
     CDS             complement(6933..7376)
                     /locus_tag="U4B19_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEN2077437.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="U4B19_001698"
     CDS             complement(7377..8165)
                     /locus_tag="U4B19_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2077438.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="U4B19_001699"
     CDS             complement(8162..9103)
                     /locus_tag="U4B19_001699"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEN2077439.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="U4B19_001700"
     CDS             complement(9280..9867)
                     /locus_tag="U4B19_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEN2077440.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="U4B19_001701"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="U4B19_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEN2077441.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="U4B19_001702"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="U4B19_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN2077442.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12784..13719)
                     /locus_tag="U4B19_001703"
     CDS             complement(12784..13719)
                     /locus_tag="U4B19_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2077443.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13905..14864
                     /locus_tag="U4B19_001704"
     CDS             13905..14864
                     /locus_tag="U4B19_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEN2077444.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(14950..16044)
                     /locus_tag="U4B19_001705"
     CDS             complement(14950..16044)
                     /locus_tag="U4B19_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN2077445.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            16233..16994
                     /locus_tag="U4B19_001706"
     CDS             16233..16994
                     /locus_tag="U4B19_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2077446.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="U4B19_001707"
     CDS             complement(17109..17549)
                     /gene="fabZ"
                     /locus_tag="U4B19_001707"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEN2077447.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            17769..18524
                     /gene="fabI"
                     /locus_tag="U4B19_001708"
     CDS             17769..18524
                     /gene="fabI"
                     /locus_tag="U4B19_001708"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEN2077448.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(18634..19647)
                     /locus_tag="U4B19_001709"
     CDS             complement(18634..19647)
                     /locus_tag="U4B19_001709"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEN2077449.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            19812..20732
                     /locus_tag="U4B19_001710"
     CDS             19812..20732
                     /locus_tag="U4B19_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEN2077450.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         6001 a         4467 c         3685 g         6646 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tcatccccta cttttaaact
    12781 cgttcaatga acatattggc ttaaaaattg ttggactact tgctgatatt tttcaggatc
    12841 tgtcttgtat gcttccgcat gctcggctcc ttttacaaca tacttttctt ttggtccctt
    12901 cgttgcacga tagaccttat ctaacatttc aaatggtaca aatgtatctt cgtctccatg
    12961 tataaagagc attgggcgct ggtttttctt caattgttcc actgcatttg cttcaccgaa
    13021 aaagtagcca gcacggactt ttgtcactag actcgttact tgcataagcg gaaatgctgg
    13081 aagactaaac atatcgtcta gctgataagc aagctcatca tgaacggaag aataaccgca
    13141 atcttcaatg attgctttca cattatctgg tagtttttct ccgctagtca tcataactgt
    13201 tgctgctccc atgctgacac cgtatagaac gatttcttgc gattttccat tgttttctag
    13261 cactttgttt atccactgta catagtcttt acgctctggc catccaaatc caatgtaatc
    13321 cccttcgctt tttccatgtc ctcttgcatc aggaacaagg acattgtaac ccatatcatg
    13381 aaacatcttt gcgtaattgg tcatcgtttc agcgtttccc atatagccat gagccacaag
    13441 agctgttttt tcacttttcg tttcagcagg aagataaatc gctgataatt tcagaccgtc
    13501 ttttgaagtg atggtccaat cgcttcggtt ttcttttgaa ctaaaccatt tatgtgcatc
    13561 cgtttctttg gatgtctgtg ttgtaccagg tgtatcttcg tctaaaaagt ctttctcaga
    13621 aggcacaaca gcataattat aaaaataatt ccctgcaaac actaacgcaa caatcagagc
    13681 taaagcgacg cctaaaagga ttttcatcca aagtttcata aatcatccat ctcatttctc
    13741 ttttttcata taaccttgaa ttagtataat caaagaatct ctactatgca atgttcattt
    13801 ttataacccg cgaattgtaa aattaagtta gaaaaataaa aaggcattta tggtacactc
    13861 aaattgtatt tccgacgaaa gaaaacaaag gagtgtaacc ataaatgacc tacaaacatc
    13921 ttaccataga cgaactgaca atgatagaat catattatct tcaacataat aagccggttg
    13981 aaatcgctaa ccgaatggga cgtgctatac aaactattta taatgtagtc aataagttca
    14041 agcaaggcaa gactgctctt gattattggc accagtataa agaaaataag aaaaaatgtg
    14101 gtagaaaagt cattcaatta cctgctcatg aagtagatta cattaaagag aaagtcactc
    14161 ttggttggac gcctgacgtc attatcgggc gaaaagaaag gtctgtttca tgcggtatga
    14221 gaacacttta tcgtttattt tctaaaggaa tatttgatat tgacacacta ccgatgaaag
    14281 gtaaaagaaa acccaatggc catcaggaaa aacggggaaa acaacagtat cagcgctcaa
    14341 tccatgatcg acctgataat tatcctgatt tcaattctga gtttggtcac cttgaaggtg
    14401 atacgatcgt tggcattcat cataaaagtg ccgtcattac tttagttgaa agattatcta
    14461 aagtcattat cacgattaaa cccaacggcc gtaaggcatt agatattgaa actgccctta
    14521 atcaatggtt ttctcgcttc cctaaaaact tctttaaatc tattacgttt gactgtggaa
    14581 aagaattttc taactggaaa gccattagta accaacatga tattgatata tattttgcgg
    14641 accctggaac accttctcaa cgcccattaa acgagaattc taacgggatt ctgcgtcgta
    14701 atggactgcc gaaatcaatg gattttagaa aagtgaatca gacatttatt tccagtgtca
    14761 gcaatcaacg taatcatatt ccaagaaaat cattgaatta cagaacacca atcgaaatat
    14821 ttttgagcta tgtacaagaa gcattttatt ctagcttaat ttgacaaatc ataacctata
    14881 aaaaaacaaa ataaaaaagc agaaataact ggctatttct agcggattat ttctgcaata
    14941 actaattatc tattttttat tttcaattaa tttaaagctt gaccatggtt tcaaaaagtc
    15001 aaggaggaat aattcgtcat cggagattct tcctacaaca ttgacacgtc cgtcgttttt
    15061 catatccttc aaggcgattt gtgtttctcc tttgtattga ccgtaccctt cattgtcaat
    15121 cagtacatcc ccatgatgca tatctcgcgt attatgaggc gggaaatctt tatctttgta
    15181 gtatacacga gtcatcgttg aacgaaggat atattctgag cgatcccctc gataactatg
    15241 aaggttatca aacaagcaga ttcgttcgtt ttctgtaata ccttcttccg tatctacttt
    15301 caatgttgga taatccgcgt taaatgcttc tgccatttct ttcaattctt cttcacttgc
    15361 gtacgcattt ccaatagaaa tatcatcaat caatcctgta aggattaaat gcttcacttg
    15421 tgtcgcaatt tcaagatctc ggtggtcttc cagcgtgcac aagccatctt gcgtaggcca
    15481 agggccaaac tcagcagatt gcgaattgac gaatgccatc gtattcaaat tatattttct
    15541 gaatttttct gaacaaaaaa caaagtgttc gtatcctaaa ccagagtatc gatgaggata
    15601 aaaattatga gaacctaata aattatccgt gtttggcgaa taactcatga tgttgtcaac
    15661 gtaattcgtg cccgaactca tattgatctc gattttgata ccaaaaggat tgcgcgtcat
    15721 ttttgcttct tctgctcctg tgaatccgat atctaagcgg accccgtagg cacccatctt
    15781 atggaaaaaa gataagtcat cgtaagaaat ctccaactgt tcaaataacg ctggattgat
    15841 atcaaccatt acttccatgc ctaggctatt cgcgtaatca actacttctt taaattctga
    15901 aagaactttt tctttatcat cattgatctg cagcaaactg gtaaaaaccc gtttataccc
    15961 atacttatga gctaaatcca agtacgcttt gtccttttca aatgttgacc gttctggata
    16021 aatcgagatt cctaattttc ccatattacc atttcctcct gcatcttttc gtttttgtat
    16081 ccacttacat tatagcaaac acaaagtaaa tggtctacac cttttctttt tcttttaaaa
    16141 taaatgataa attccaataa tttaagtagg tattttccct ctattttgtt atcattaaag
    16201 ataacaaaat agtaaattgg aaaggaacag ttatgaagaa aaataaagtt attttaagta
    16261 ttagtttagc agcagcattg ctgtttgctg ctggttgttc tgcaaacaac acgacttctt
    16321 ctagttcgtc tacagaaagt tcaaaaagta catctactgc ggaaagcacg actgccagta
    16381 gcgtcgattt gaacgcattg gaattaccac aattgacgaa agatgtcgcc gaaaatgaag
    16441 atctggtcga aatggtgacg aataaaggca caatcaaaat caaattattt cccgaacacg
    16501 caccaaaagc ggttgaaaac tttatgacac atgcaaaaga cggttactac aacggactga
    16561 ctttccatcg cgtcatcaaa gatttcatga tccaaggtgg cgatcctaaa ggcgatggca
    16621 ctggagggga aagcatttgg ggagaaggat ttgaaacaga gatcaacaac cacctttata
    16681 atataagagg tgcgttatct atggctcgct cacaagatcc taacagtaac ggcagccagt
    16741 tctttatcgt tcaaaataca gatgatatgc atgatggtct tttaaaagac gactatcctc
    16801 aagcaatcat cgacgcttat aaaaacggcg gttatccttc actagatggc cattatactg
    16861 tcttcggcca agtgattgaa ggaatggatg tcgtggatgc tattgcaaat atcgaaacag
    16921 atagttcaga caaaccaaaa gaagaagtaa aaatcgataa aatcaatata ctgcaagaag
    16981 caaaacaaaa ataaaaaaat aacgtctgaa tcctgcatag atgacaacgt atgctaggat
    17041 tcaggcgtct tctttttaca taaaaagcaa gtaagcttcc ttttgttttg ctatatcagg
    17101 ttttatagtc aagcattttg gttgacgatg aacgtgaact cgcactcaca gactttttta
    17161 tcatctacat atgctgcaga taacgctgta ccgactgatc ccttcatttt ggtgatttca
    17221 aaatgacatt tcagcacatc tcctggaaca actttttgac ggaatttggc cttattgatc
    17281 ccaccgatat aagctctttt tcctagaaac tcttctgatt ttaggataag tatcgaacct
    17341 gcttgtgcca gtgtttctag aatcaacgca cccggcatca ctggattgtc aggaaagtga
    17401 ccttggaaaa aatcttcatt gattgttaca ttttttgtcg caacgatctt ttttcctggt
    17461 tctaaagtat ctacataatc aatataacaa ataggataac gattaggaat caattccatt
    17521 acttcagtag cagataatac gtaagacata attaaactcc ttctttcagg caattgccat
    17581 tttcattact ttgactatca aagtatcata ttcctaaata tttagcaatc tttttttaat
    17641 ttaagaaaaa atagtttgac aatcaaaaca ttttgactaa taattaagaa atcaagaagt
    17701 tattttagct attattctct atttttttac gactatcaaa tttttatcag aaacaaagga
    17761 gagtcattat gtcatttcta gaaggaaaaa aaatcgtcat tatgggcgtc gctaacaaaa
    17821 aaagcattgc ttggggttgt gctgaagcaa tgttgaaaca aggggcagaa attatctata
    17881 cttaccaaaa tgaacgaatg aaaaaatcat tagtcaaatt ggtcggcgaa gaagcattca
    17941 ctgtagaatg tgatgtttct agcgacgaaa gcatcgaaaa agcaatgaat gccatcggtg
    18001 aatatgctgg ggagatccac ggccttgtcc atgcagttgc ttacgccaac aaagaagaat
    18061 tgtccggaaa tgtgtctgat atttccagag acggctatca attagctcaa gatatcagca
    18121 gctactcttt aattgctgta tcaaaatacg cacaaccgtt tcttgcacca aacagcggga
    18181 tcgttagcat gacttatcta ggttcagaac gcgcaatccc gaattataac atgatgggta
    18241 ttgcgaaagc ttctcttgaa gctgctatcc gttatttggc agctgagttc tcgcctaaag
    18301 gaattcgggt caatggaatc tcagctgggg caatcaaaac attagctgtc acaggtgtta
    18361 aagactatca aaaactgatc cagttatctg aatctcgtac gcctgatcag caaggtgtga
    18421 cgatcgaaga agtcggtaat gcttgtgctt tcttaatcag cccactttcc agcggaatca
    18481 tcggtgatat tatctacgtt gacaaaggtg tccatctttc ataaactttt gttgcattct
    18541 ttaaagtaaa acatataaaa atcaggaaaa aactttttaa agtctcttcc tgattttttc
    18601 tttttacgtt tttttcaact cattactttg aaattattcc acgacttcaa tccagccttc
    18661 tggtgcttct atgtctccaa attgaatacc tgtcaattca tcatacaacc gttttgttac
    18721 tggtcctact tccgtttcac tataaaatac atgaagattg ttattgtgat aaatcccacc
    18781 gattggagaa ataacagccg ctgtaccaca tgcaccagct tctgcaaatt gatctagatc
    18841 atcaatgaaa atatctcctt caattgcttg cagtcctagt cgttcttcag ctaactgcaa
    18901 caaggaatat ttcgtgatac ttggtaagat agagggcgat tgcggagtga tgaattttcc
    18961 gtcttttgta attccaaaga aatttgctgc acctacttct tcgattttcg tatgtgtggc
    19021 tggatcaaga taaacacaat ctgagtaatc cagttctttt gcttctgttc ctggaagtaa
    19081 actcgctgcg taatttccgc cgacttttgc tgcaccagtc cccttaggag cagcacgatc
    19141 gtaatcagat acaataaaat tcgttggttt tagtccacct ttgaaatacg ctcctactgg
    19201 cgtacagaag acgacaaata aatattctgg agctggatgg acaccaatgt tttcaccgac
    19261 cccaatcagt aaagggcgca agtataatgt agcgcctgtt ccatagggcg gcacaaaatc
    19321 ttcatttgct tttaccactt gcttgacagc atcaacgaac atcttttcag ggaccattgg
    19381 catcagcaat cgtctagcac tttgattcat ccgtcgacta ttttgttcag gtcgaaagag
    19441 attgaccttg ccgtcttttc gacgataagc ttttagacct tcaaaacatt gctgaccata
    19501 atgaagcgct ggagagcctt catgaatcgt caggtaatta tcttctgtca attctccttc
    19561 ttcccattgt ccgtctttcc atttggcaat aaaacgccaa ggagttttga tataagaaaa
    19621 acctagattg ttccaatcga tttgcatata cttaccacct tcgttatttt ctccacattt
    19681 tatcattatc catattattt gtcattttat tttcagcttt tttccttcat tatagttatt
    19741 tttgctattt ccttttttga aaacttcttg gtacaataca gaaagaatca aattttagaa
    19801 aagaggattt tatgttttca ttattagcaa ctgccggtac gacagactct tctggcgaga
    19861 cattgacaga tcaagctgtc agcaaagtaa atgcgtttca gcggttttgg gacgggattg
    19921 attgggacgc cattttggct gcctttatcc aaaaaagttt atatctgatt tttttgatcg
    19981 tccttttttg gattcttcac cgtgtgggaa aatatctgat tgataagtct tttaatcagt
    20041 attctaaaaa agccattcta aacgagagcc gcttaaagac tttacatagt cttttaaata
    20101 ctatttttca ttataccctt ggtttctttt ttatctatgc aattttatct gctatcggtg
    20161 tgccgatcgg atcacttttg gctggtgccg gaattgcagg tgtggcaatc ggtcttggtg
    20221 cccaagggtt catgagtgat gttattaccg gattttttat tatcatggaa caacagatgg
    20281 atgttgggga ctacatcaag ctagcaaact taacgattga aggaaccgtc gtatctgttg
    20341 gtatccgtac ccttcagctg aaagcaacgg atggtactgt ccatttcatc ccgaatcgaa
    20401 acatcaccac gatcagcaat ctctctcgtg caaatatgca agtactttta gatattcgga
    20461 ttgttcctga agaaggatat gacaaaatct atgagatcat tgatcgagta aatcgtacat
    20521 tagctgaaaa ataccaagaa gagctccaaa ctgaaccttc tatctttgga ttagtggatc
    20581 ttggaaacag caattttgcg attcgtacgg tctgctacgc attgaacgga aaacaatatg
    20641 ccttaaaaga agagttcttg agctcctatg tcaaagaatt gacagcttct ggtttcacga
    20701 ttccgaacag tccaattgct gtcggaaaat aatttggaca aacgatgcaa cgaatctcac
    20761 taaaaagagc gtgggacaaa agtaaagaac acttttgtc
//
LOCUS       DASJTC010000053        19629 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.053, whole genome shotgun sequence.
ACCESSION   DASJTC010000053 DASJTC010000000
VERSION     DASJTC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19629
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.053"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="U4B19_001711"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="U4B19_001711"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEN2077451.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="U4B19_001712"
     CDS             complement(1172..1867)
                     /locus_tag="U4B19_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEN2077452.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="U4B19_001713"
     CDS             complement(1880..2596)
                     /locus_tag="U4B19_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEN2077453.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="U4B19_001714"
     CDS             2926..3534
                     /locus_tag="U4B19_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEN2077454.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="U4B19_001715"
     CDS             3859..4182
                     /locus_tag="U4B19_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN2077455.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="U4B19_001716"
     CDS             4364..5734
                     /locus_tag="U4B19_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEN2077456.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="U4B19_001717"
     CDS             complement(5864..7165)
                     /locus_tag="U4B19_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEN2077457.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="U4B19_001718"
     CDS             complement(7519..9477)
                     /locus_tag="U4B19_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEN2077458.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="U4B19_001719"
     CDS             complement(9654..9920)
                     /locus_tag="U4B19_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2077459.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="U4B19_001720"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="U4B19_001720"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEN2077460.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="U4B19_001721"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="U4B19_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEN2077461.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="U4B19_001722"
     CDS             13264..13827
                     /locus_tag="U4B19_001722"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEN2077462.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="U4B19_001723"
     CDS             complement(14080..14601)
                     /locus_tag="U4B19_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEN2077463.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="U4B19_001724"
     CDS             complement(14604..15446)
                     /locus_tag="U4B19_001724"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEN2077464.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="U4B19_001725"
     CDS             complement(15439..16164)
                     /locus_tag="U4B19_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEN2077465.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="U4B19_001726"
     CDS             16340..16636
                     /locus_tag="U4B19_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077466.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="U4B19_001727"
     CDS             17204..18796
                     /locus_tag="U4B19_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN2077467.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="U4B19_001728"
     CDS             18809..19549
                     /locus_tag="U4B19_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2077468.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5617 a         4028 c         3724 g         6260 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagccattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaagtaaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgagatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttc
//
LOCUS       DASJTC010000054        19476 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.054, whole genome shotgun sequence.
ACCESSION   DASJTC010000054 DASJTC010000000
VERSION     DASJTC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.054"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..3654
                     /locus_tag="U4B19_001729"
     CDS             127..3654
                     /locus_tag="U4B19_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEN2077469.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="U4B19_001730"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="U4B19_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEN2077470.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="U4B19_001731"
     CDS             7442..7783
                     /locus_tag="U4B19_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2077471.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="U4B19_001732"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="U4B19_001732"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEN2077472.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="U4B19_001733"
     CDS             9140..11560
                     /locus_tag="U4B19_001733"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEN2077473.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="U4B19_001734"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="U4B19_001734"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEN2077474.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="U4B19_001735"
     CDS             12994..13731
                     /locus_tag="U4B19_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2077475.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="U4B19_001736"
     CDS             13745..14560
                     /locus_tag="U4B19_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEN2077476.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="U4B19_001737"
     CDS             14573..15220
                     /locus_tag="U4B19_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2077477.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="U4B19_001738"
     CDS             15236..15892
                     /locus_tag="U4B19_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2077478.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="U4B19_001739"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="U4B19_001739"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEN2077479.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="U4B19_001740"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="U4B19_001740"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEN2077480.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="U4B19_001741"
     CDS             18234..18752
                     /locus_tag="U4B19_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2077481.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="U4B19_001742"
     CDS             18859..19359
                     /locus_tag="U4B19_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEN2077482.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6553 a         3344 c         4182 g         5397 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaagga
//
LOCUS       DASJTC010000055        19351 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.055, whole genome shotgun sequence.
ACCESSION   DASJTC010000055 DASJTC010000000
VERSION     DASJTC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.055"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(39..635)
                     /locus_tag="U4B19_001743"
     CDS             complement(39..635)
                     /locus_tag="U4B19_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2077483.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            985..1329
                     /locus_tag="U4B19_001744"
     CDS             985..1329
                     /locus_tag="U4B19_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEN2077484.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1330..1749
                     /locus_tag="U4B19_001745"
     CDS             1330..1749
                     /locus_tag="U4B19_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEN2077485.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1841..2191)
                     /locus_tag="U4B19_001746"
     CDS             complement(1841..2191)
                     /locus_tag="U4B19_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077486.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2157..3488)
                     /locus_tag="U4B19_001747"
     CDS             complement(2157..3488)
                     /locus_tag="U4B19_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEN2077487.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            3743..4309
                     /locus_tag="U4B19_001748"
     CDS             3743..4309
                     /locus_tag="U4B19_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2077488.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            4454..4541
                     /locus_tag="U4B19_001749"
     tRNA            4454..4541
                     /locus_tag="U4B19_001749"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4486..4488,aa:Ser,seq:cga)
     gene            4877..5851
                     /gene="bsh"
                     /locus_tag="U4B19_001750"
     CDS             4877..5851
                     /gene="bsh"
                     /locus_tag="U4B19_001750"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN2077489.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            6176..8071
                     /gene="asnB"
                     /locus_tag="U4B19_001751"
     CDS             6176..8071
                     /gene="asnB"
                     /locus_tag="U4B19_001751"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEN2077490.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8266..8712)
                     /locus_tag="U4B19_001752"
     CDS             complement(8266..8712)
                     /locus_tag="U4B19_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEN2077491.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            8940..9089
                     /gene="rpmG"
                     /locus_tag="U4B19_001753"
     CDS             8940..9089
                     /gene="rpmG"
                     /locus_tag="U4B19_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2077492.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9177..9695
                     /locus_tag="U4B19_001754"
     CDS             9177..9695
                     /locus_tag="U4B19_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2077493.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(9772..10728)
                     /locus_tag="U4B19_001755"
     CDS             complement(9772..10728)
                     /locus_tag="U4B19_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2077494.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            10894..11700
                     /locus_tag="U4B19_001756"
     CDS             10894..11700
                     /locus_tag="U4B19_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077495.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            11697..12686
                     /locus_tag="U4B19_001757"
     CDS             11697..12686
                     /locus_tag="U4B19_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN2077496.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            12683..13687
                     /locus_tag="U4B19_001758"
     CDS             12683..13687
                     /locus_tag="U4B19_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEN2077497.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            13819..14361
                     /locus_tag="U4B19_001759"
     CDS             13819..14361
                     /locus_tag="U4B19_001759"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEN2077498.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14381..15079
                     /locus_tag="U4B19_001760"
     CDS             14381..15079
                     /locus_tag="U4B19_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEN2077499.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15100..15312
                     /locus_tag="U4B19_001761"
     CDS             15100..15312
                     /locus_tag="U4B19_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEN2077500.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15312..16274
                     /locus_tag="U4B19_001762"
     CDS             15312..16274
                     /locus_tag="U4B19_001762"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEN2077501.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16292..16687
                     /locus_tag="U4B19_001763"
     CDS             16292..16687
                     /locus_tag="U4B19_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEN2077502.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            16850..17215
                     /locus_tag="U4B19_001764"
     CDS             16850..17215
                     /locus_tag="U4B19_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEN2077503.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17212..19023
                     /locus_tag="U4B19_001765"
     CDS             17212..19023
                     /locus_tag="U4B19_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEN2077504.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19085..19252)
                     /locus_tag="U4B19_001766"
     CDS             complement(19085..19252)
                     /locus_tag="U4B19_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEN2077505.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
BASE COUNT         6260 a         3295 c         3940 g         5856 t
ORIGIN      
        1 aacattgtca atcaatgact aattttacaa aataaatatt atccaagaag accacttaga
       61 tattgacctc cataaatcaa ggccatacta tataagaaaa tcgacagtgg tttagcaatc
      121 aaaatgatta gtgtatatcg tttcactgac atgtttgtca atccagccat caaacataac
      181 gcatcgtcag gagcaactgg aagaaaaata gctagagcaa acagcttctc aaaacggttt
      241 tgattatcca gccagccaat atatttgtta tatgtcttat cgctaatcat ggaaagtatg
      301 aatggctttc cataacgacg ccctagcaga aaaatgataa tcgaaccaat gcagattcca
      361 atatagttat agataaatcc agcataaggg ccaaatatca ggacaccagc tgcagtactg
      421 atccctccag gaatgattgg aataactact tgcaggattt ggatgaagac aaaaataatc
      481 ggtcctaaaa tgatggaatc acctactaat cctcttaacg cattcaaatc tttgaaaaca
      541 cctaaattaa taaaataaat cgtcagcgcg attgataggc ctaatccgat aatggatatg
      601 aaatttatca gttttcgaga ggcagcaata ttcattattc atcaactcct tagaggttct
      661 ttgttaacta tactttacta gaaaagcaac taactgcgcc attttttgat atgacagaaa
      721 acaaacaatt ttgtaagttc atcataagta taaaagattg atctcaaaaa aacatccaac
      781 taaagactta tttaataact tgtttttttt aaaataaaaa aagcgttagc aactatgctt
      841 aaaaagcata tcgactgaat aaactaagca ttggacaggc tgaaagacta catttaaaat
      901 gaaatgcgaa gaaacaatag aaaataaagt tgccttagaa ccatagttga atcacctaac
      961 agtcgtgaag ggagaataag agaaatggca gaaaaacaaa ccgcaggacg agatcaatta
     1021 ggagattttg caccaaaatt tgcagaatta aatgatgatg ttttatttgg acaagtatgg
     1081 tccagagaag aattatctgc tcatcagcgt agtatcatca ccatatctgc attaatttca
     1141 ggaggaaatt ttgaacaatt gccagctcat ctgaagattg gcaagcaaaa tggcattact
     1201 agtgaagaaa tagcagaaat cattacccat ttagcttttt atgtagaatg gccaaaagct
     1261 tggtcagctt ttagtttagc aaaagaaatc tatttagaag aaaatagcga agataagaaa
     1321 gaggcgtaaa tgatgacaaa acacgaagaa atacacaatg gcgtgatttt tccagcaggt
     1381 gaaaaaaacg aagcttacgc tcaagtattt accgggcaaa gctatctgaa tatgttagtg
     1441 aatgatcccg aagtttctgt tggtgtaggt aatgtaactt ttgaaccagg ttgtcgcaat
     1501 cactggcatg tccatacaga cggctatcaa atcttacttg tgaccggagg agaaggctgg
     1561 tatcaggaag aaggaaagcc agcaagacga ttaatggctg gagatgtcgt cgtaacgcac
     1621 aaagggatta aacattggca tggtgcgaca aaagacagtt ggtttgaaca tttggcgatc
     1681 actgctggta attcagagtt tttagaacct gtaacagatg aagtgtatga aggattggaa
     1741 aatcaataat gtcaagcttg aacagctcct gaaagtagat tttaatcgaa ctttcaggag
     1801 ctgctttttt gattcagtct tttataaata gaaaataggg ttaataatag gaatgagaag
     1861 cacgtcgaaa gatcttaata ttgataatcg gctgggcata catgatttcc acgtcatcaa
     1921 tcaaactacc taacagatga taatcttccc gatcatgtcg gggattacct aatagttctt
     1981 gatagtagat ttccatacta gggtcccgag gattgttcag caaatcagca agtacatcta
     2041 aatcttctgg ctgagtttct gccgtaccct gtatctctat gaaaagccct tcctcagcaa
     2101 attctactcc aatacgtatt tcagttattt catgggaata taaataagta agaagctcat
     2161 cgatgattct ttttgataat tctttttcca agtgtctttt tttcctttct gacagcggta
     2221 aagttatcta gtatcggtac aaggatacct gcaacgaaac cagttgaaaa accactgtta
     2281 tatagattta gtcctccgtg caaagtgctg acgttatgga caagagcaat atgtagcaca
     2341 ccagctatta caccgtaaaa actgccgtaa aaaccgctta tgggcgcaag acctgtccca
     2401 aacacagctg caacaagcat gccagtactg gtgatctcat gaaaagttcc aaacaaagaa
     2461 gcaataaata ttccgactag tatcggaaaa ctgtttttta aatgacaacc aaaagcactg
     2521 aaacccaccg cagataaaat agcaccgatg acaggtccat ttaactgacc gccaaccagc
     2581 aaaacataag aagacagcat cagaccaact aagcccatgt tgatcaaagt agcaccaata
     2641 ccagaattag caatgaaatc agttgtcaat tttccagatg aatcgaaaat ttctcgaatc
     2701 cccgaaagcc gaaaggaatt ataatagaaa ccaattccga ctgtcagcga aaaaaagcca
     2761 aaaataatcc aaatgagagg aaagtgatat tcattgaaaa ccaatgtatt ttcgacgatt
     2821 gaatagctga ataacctcaa aacagcagta aaagtcatcg caatcaaccc agaggtgaat
     2881 ccaatattat aaagtgtgaa tccacgatgg aaaactaaag tttgagcagc taagggcgga
     2941 agaagtatac caattgctat gccagctaaa ttccccaata aaatacctat cagtaaagga
     3001 aaacgcatac caaaagtgat ataactaacg accggtgata atacgctacc aaacagtccg
     3061 atcatgatgt actgagaaaa aggtttattg acaaatacag cgtatagata cacacctaaa
     3121 atgattgaga tagaattata aaggtttttg ccaaaaaaag agaaaccggt aactgttaga
     3181 atcgaagcaa ataagggacc gtttaaaatc accttgtgcc gataagctaa aagcacagaa
     3241 agtaaggtca aaatccctac atttatgaat gcgcttccga aaacacctaa agcaaaataa
     3301 tctgtcaaaa gattgcttgg cgaggtaaga atagttagca atccactcca attatcagta
     3361 ggagtattag aaagcaagcc aataaaaaga aaaaaaacac ctgtaaaacc aagataaaaa
     3421 tactttcttt tctgtgaaac gattcgtttt tgaggatgaa tataaataac gtcagtttcg
     3481 ttttccatta cacaccttct ttttcatttt ctttaactat aacttgaaat gaaagatttg
     3541 gacaactatc atttaggtca aaactaaaaa tgtaagggaa cccattgaat ttaaccaaat
     3601 ttttttaatt ttgcaaaaaa aagttaaaga agactgaaaa aaatttaaaa tataacgttt
     3661 tgagtaaaat gtttgcaaaa aatgctaaaa atagggtaca ataaaacaac atatactgca
     3721 gtaaagaagt gggggagttt taatgagaag aaaagtctat accaaggatc aaattctaaa
     3781 agctgcttat gatgttgttg caaaggaagg gtttaaagga tttactgcga gaaatattgc
     3841 taaaaagatg gggatttcga cacagccgat ttatctggaa ttcaaaaaca tggaagattt
     3901 aaaaaatacg ttgctggatt ctatctttga acaactaaaa gaagatgttt tccctattga
     3961 acgtactggt gataaaatca ttgatctcgg tttgaactat atctactttg caaaaaagca
     4021 tcatagtctt tacatggcat tgtacattga tgaacatggg gacggaaaga aaatgcatga
     4081 tttttcatac aattatttcc gtgaaatagt taaagaagat gaaagataca atcaattatc
     4141 ggatgaatat gtcgatgctc tgcatactgg tacatgggta gcagtgactg gcttagcgtc
     4201 attgatgtca tcaaacatca tgcatccaac agaagaacaa atcattgcac tgatcaaacg
     4261 gacaattgac tcgattttag cattagataa tcctgaaata atcagatagt gttctatcag
     4321 tatgaatttg atcggtaagt ccgttggctg caaactgtca atggactttt ttgctaaaaa
     4381 aagcgatttc tgttatttat ctattagaaa taggcttgac atttcgtgaa aaaagtttaa
     4441 aaatagtact tacggaaggt tggcagagtg gtaatgcacc ggactcgaaa tccggcgaac
     4501 cggctaatac cggcgcgcag gttcaaatcc tgtaccttcc ttatatcaac gattagaggt
     4561 tgtgaagaag ctgtcctgca tcaagtaata agaacggcca aacaaaaata gcttttccta
     4621 ttttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttgttcgg
     4681 aataaggggc tgtgacaaga cttttgtcac agccccttat tgttgtactt ttaacctaca
     4741 atattttttg attccccctg agtactattt tttataaatg ctagtaaatc aatcttcttt
     4801 ggcctttttt ttgataaaaa aaaatactag atgtaagata aaagtgtaaa aacgatttgt
     4861 tttaggaggg aaaacgatgt gtaccgcaat cacttatgca acgaaagacc attattttgg
     4921 aaggaatttt gattatgaga tgtcttataa tgaagtagtc actattactc caagaaatta
     4981 tcgtttcgat tttagaaagg taaaaaattt agataaacat tatgcaatga taggaatagc
     5041 agctggggta tccaactatc cactctatta tgaggctaca aatgaaaaag ggttgagtat
     5101 ggcaggactg aattttcctg gcaatgctga ttataaagaa ttacaagaag gaaaagataa
     5161 cgttgcacca tttgagttta ttccatggat actagggcaa tgctcaacca tagacgaagc
     5221 taaagaatta ttggctacta tcaatctagt caatatcgat tttagtgaaa aactaccctt
     5281 gtcaccttta cactggttat tagctgataa agaaaaatcg atagtaattg aaagtatgaa
     5341 agatggactt catttatatg ataatcctgt cggtgtgttg acaaataatc cgccatttga
     5401 ttatcaatta tttaatctga acaattatcg ctcgctatca aatggaacac cagaaaatca
     5461 tttttcaaac cagatcagct tgaatgttta tagtcgtggg atgggaggat tgggcttacc
     5521 tggagatctc tcttctgttt ctcgatttgt aaaagccaca tttactaaaa tgaatgctgc
     5581 atcaggtgat tcagaatctg aaagtatcag tcaatttttt catatactag gctcagtcga
     5641 gcaacaaaaa ggagtatgcg atactggaga aggaaaatac gaatatacga tttattcttc
     5701 ttgctgtaac gttgataaag ggatttatta ttatcgtacg tatgaagaca gtcaaattac
     5761 tgctgtaaat ataaataaag aagacttgga tcgtcatgaa ttggtcagtt atccagtcat
     5821 aaaagagcaa cagataaact atgtcaatta attaatgaat agatccgttt ggtatcatat
     5881 tttctgatac agtcataatt agacacgatc tttaggtcgt gtcttttttt gctgtttttt
     5941 ttggaaatag catccactaa tattttttta acacgaagag aataaggtag cctcaaaaaa
     6001 gaaaacatgg taaattagat attgggactt tattttctgt aaaaacagaa actttcatct
     6061 tttgttttca cgtgacgaaa ttgtaagaga gaaaatattc gtccgctatt tattctatga
     6121 taagatgcat atgaatgaaa taaacagtaa ttttatatta ggatgtgaaa gttaaatgtg
     6181 cggtattgta ggttttgtta acgacaagga caacaaaaaa acaattatta atacaatgat
     6241 ggatcgaatc gttcaccgtg gacctaatag ttcaggagaa tatatcgata aacatgttgc
     6301 tttaggattc agaagattaa gtattattga cttagagggc ggtactcagc ctatttacaa
     6361 tgaagatcgg acaaagatca ttatttttaa tggcgaaatc tataattacc agcctttgag
     6421 agaagaattg attgcagctg gccacgtttt tcaaacacat gcagatacag aagtcctttt
     6481 gcatggttac gaagaatggg gaacagaatt attgcaaaag atccgcggaa tgtttgcttt
     6541 tgctatttgg gataatgaaa agaatgagtt attcggtgca agagaccact ttgggatcaa
     6601 accatattat tatgcagaaa tgaacggaac atttatgttt ggttcagaaa tcaaaagttt
     6661 tctgcctcat cctgatttta acaaagaatt gaacaaagaa gcgttgaaac cttatatgac
     6721 tttccaatat tctccattaa acggcgaaac attcttcaaa gatgtctatc gtttgcctga
     6781 aggccattat tatacgtata aagatggaaa actagacatt cagcaatatt gggatgcaga
     6841 ttttgaaaca aaagaaacgc attcccgaca agaatggatc gaaaaaatcg acgaaaccgt
     6901 tcaagcttct attgaagcac atacggtcag tgatgtagaa gttggttctt tcttatccag
     6961 cggggttgat tccagttatg taacctctgt attgaaacca gatcattctt tctcaatcgg
     7021 gtttgatgat aaaacctata atgaagcgat tgaagccaga aaactaaccg aactattgga
     7081 cttagataat acagcagcag tgatcgacgg agacatgtcg ttcaaagcat tccctttgat
     7141 ccagtatcac ttagatgaac ctgattcaaa tccttcttgc gtacctttgt actttttagc
     7201 aaatcttgct tcacaaagtg tgcgggtagt ccaatctgga gaaggggcgg atgaactgtt
     7261 tgcgggatac caaacgtatg gattccatac gaattcaaaa ttcatccgtg tgattgctca
     7321 aggattgaaa aaattaccaa aaggaacacg ctataattta ggacgtaaaa tcggaaaaat
     7381 gaaaaatttc catggacgga tccatttata tgaatcactt gctcctgcaa aagaatattt
     7441 tatcggtcat gctcgagtgt tcgaagaatc agaagcatca gaagtgttaa caccaaaata
     7501 tcaaacagca ccatctgtgg atgaaatcat gactgttcat tacgagaaaa cagaaggaat
     7561 caaagatgaa gtcaataaga tgcaatatgt cgatcttcat caatggatgc caaaagatat
     7621 cttgttaaag gcagataaac tttctatggc aagttctttg gaagtccgag tacctttatt
     7681 ggatatcgaa gtcatgaaat tggctcaaca aataccaagt aagtttttat tgaatcaaaa
     7741 caacacaaaa gatattttca gacaagctgc gaacaaacat cttccggaag aatggtcgaa
     7801 tcgtgtgaaa ttaggattcc ctgtgccaat caaagcttgg ttgaaagaag aacacggcta
     7861 cgaacaagtc aaagcattat tcgaagcaga ttttgcaaaa gaattttttg atcaagaaaa
     7921 aatcatgaag ttgctagatg accatcacga aggacgtaaa gaagagcaaa gaaaaatctg
     7981 gacaatcttc agtttcttga catggtataa agtctacttt gtagatgaaa cgattccgca
     8041 agcagaagca atcgactatg tgactgtttg atgaaaatca aatatcaata aaaaagaggt
     8101 tttctcctac cgtttaacac tgagtgataa gaaacaattt caaaaaacag ctcttcccgt
     8161 tttatagata ttatgactta tggttcggag ttggcagagg aacaccattg tttcgaacat
     8221 aagggggttg tgacacgatt ttcgtcacaa cccctttaca tatgttcaat aatcgatttc
     8281 tttttctttt gtcccatctt ttttataaat gaacaaagtt gattctttgt tttgtgctac
     8341 ttgctttcct ttttcgattg cttctttttt tgtatcaaaa tggttgcttg ccttttttgc
     8401 ccctttagat ttcacgatcc attgttcttc ctcatattct acgatcacat ctgaatcaag
     8461 cagttttcca gcacgaggat tcgtatcgtg ttcatccgtc ttgcttggat ttttttcttt
     8521 ttcgaaagtt cgtttttctg attcactagc atcttcatac catttttttg cttgatcaat
     8581 ggcaattggg atcgcacggt tatcagggta cccttcatct aataatgcat ttgcgatgtc
     8641 gattgctttt tttcttgtca atttatctaa gtttttcatt gatgcaggaa agtccttcat
     8701 gttccatggc atattagtat ccccctttat taaaaaacat aatctatata aacaagtgta
     8761 agggtattta tacaaaaaga aaaagatttt gtttgagaag taaaaccgtt gtatttatcg
     8821 tcaagtagtt ttacttaaca aaatcgtgaa aaacactagc aatctagaaa aaatcatggt
     8881 aagataatca agtctgagag tcatcttaga ggattcttgt aaaaatggga gggaaaaaca
     8941 tgcgcgtaaa catcacttta gagtgtactt cttgtaaaga acgtaactat ctaacaagca
     9001 aaaacaaacg taacaatcct gatcgtttgg aaaaacaaaa atattgccca cgcgaacgca
     9061 aagttacttt gcaccgtgaa acaaaataat gtttttaaag ttctcaattc cttgctggtc
     9121 aaagaattga gacttttttt atctcaaaac atctaaaatc ataaggagcg gataatatgt
     9181 ttatttccat gtgggcacaa gataaaaatg gtctgatcgg caaaaatggt ttgttaccat
     9241 ggcgattgcc aaatgatatg cgtttctttc gcgaacatac aatggatcga attttagtga
     9301 tgggaagaaa gacctatgaa ggaatggggg atctttcact tccttatcgc catatcatcg
     9361 tgttgacaac gcaaccaaat tttcgcacga aagaaaatgc ggaagtaatg cattcgatcg
     9421 atgaactttt gtcgtatagt caaacgatct cagaagatat ctatgtttcg ggtggtagtc
     9481 ggatttttca agaacttctt ccttacactg gtcggatctg gcgaacattg atcgatagca
     9541 cgtttgaagg cgatacgtat attgggaata ttgatttttc agacttcgcc cttatagagg
     9601 agtatgaggg gataacggat caagaaaatc tatatgccca tcgttttcaa aaatgggaaa
     9661 gagtaaaaaa tagggttgct tcaaaagaag aataaaatct atgcgtctag tttgttaata
     9721 agaaaaaaag catctcacga ctaatcgtga agattctttt tttagttaaa atcaatttag
     9781 tttttctttg atatcattga ccatttcctg actggcaata ggtccgttat atagccaact
     9841 agattcaggt ccgaattgca gtacatgatt gtctgtcact gctttaagat tcttccagct
     9901 tggttcattg aacatggcag catctttgtc actgttcaca aggatgatgt agtcagcatc
     9961 taattcagct agcttttcag aggaaacagc agaccagtca gaagtggctt ttttggagac
    10021 ctcttccact agatcaggaa caccaaattc cagatcgtcg tatacgattc ttccacttga
    10081 ccgatttttg ctgaccataa aggctgagtt gttggtcacc cacaacactg cagctgtctt
    10141 gttctcgatt ttatctgcta gttcttctcg agttttcttg atggattttt gataatcgga
    10201 aatgattttt tctgcctctt tttcttttgc caaagctttg ccgacatctt ttagttgttc
    10261 ttcccatgtg acatctgttc cgttttttac aacataagtt ggcgcgattt tgctatattg
    10321 ttcgtatttc ccgccttcga cagtagcgga agaagaaatc aacagcaaat caggctcaaa
    10381 gcttaacact ttctcataag gaaggtcata agaaatcgtt gggacatcct ttaaatcatc
    10441 ttgcagataa tcttgaatgg aaccactgcc aacagtccac tgggcgatag gtttttcccc
    10501 tagtgcaatc agatagtctt ctaagtaact gccgatgatc cgtttgggcg cgtttggaat
    10561 ctctacttta tgtccaagtg tatctgtgac tgtatgagta ctattggatg aagtcgtcga
    10621 attggcagtt gtagctgttt ctttatctga agagggggag caagcgctca gtactccagc
    10681 cagcagcaat atgctagata atgtgaatac ttttgttttt aatttcattt ctttcctcct
    10741 aacataattg agaatctttc tcattggtta ttattcctga tatttcgaac gctgtcaacc
    10801 gcttcatttg gctatagata tgaaagtagc gcttgacgaa atgttgaaac agcgtcataa
    10861 ttgagaatga ttttcagcga aaggatgggt gaaatgacga gattaagagc agctgacatt
    10921 gcagccggct acgaaaacaa aaaaatcatt gaaaacatgt ctgttacgat tcctgaaggg
    10981 aaaatcacta gtttgattgg gccaaatgga agtggaaaat ctactttatt gaaaagtttt
    11041 acccgtatac tacctgtgaa atcaggaaag atcattgtca ataactctga tatctctaca
    11101 tacagttcaa aagaacttgc aaaaaaaatt acattattag cacaaactag tgaacaccct
    11161 acaggtatga cagtagaaga agtagtatct tacggacgat acccttatca aaaactgttc
    11221 agtggcatgg acacgcaaga tcatgaagca atcgagtggg cgttggatgc tacaggtcta
    11281 gctggatttc gtatgcggga tatcgcaacg ctgtctggtg gacaaaccca gcgtgtctgg
    11341 atcgctatgg cgcttgctca ggaaacagat atcttgatct tggatgaacc gacaacattt
    11401 ttagatccgg cacaccaatt agagatccta cagttattga ctgaaatcaa tcaaatgaaa
    11461 caaacaacga tcttgatgtc gattcatgac attaatcatg cttcacgttt ttcagattat
    11521 ttattagcaa tgaaaaacgg taagttgatc acagaaggta cacctgaaga agtgatcaca
    11581 aaagagtgta tgaaaaaaat atttgagatt gatgcaacga ttgtcacgct tccaagaaca
    11641 aataaaccag tttttctaac ctatgatttg ttgccaaaag agcaggagga tcaattatga
    11701 ggactatgaa gaaaaataga ctcctgcttt tggtctgttt tgttcttttg attatttttt
    11761 ttgttgctct ttcaaatggc gcagcgactg tgggaaaagc agatttttgg caggcgttgt
    11821 ttgattttga tagatcaaaa caaagtcacc aaatcatacg aaatattcgt ttgcctcgtg
    11881 tggcgggagc atttcttgta gggagttgtt ttgcattgag cggtgcattg atgcaaggag
    11941 tgacaagaaa tcctttagca gattcaggct tactaggaat caacgcgggt gcttcgttgg
    12001 caatggccct tagttttgct ttttggccta aagcttctga tttttctgtg tttctaatgt
    12061 cattggcagg ttcttttgct gtaactttgc tcgtatttgg ttttttgcag ttttcgaaaa
    12121 tgggcatgaa tcctttacag ctgattttag ctggcgtagc tattagttct ttttttactg
    12181 ctatcagcca agcacttacc caattcttta atctgcagca ggatcttgct ttttggtttg
    12241 taggcggagc tgccaatatc acatggaaac aattagcagt attgtttcct ttatatatac
    12301 ttgctgtttt ggtttctttt tcattaggaa aagcaatatc tttgattcat ttgagtgaaa
    12361 cacatgcatt agctcttgga agtcatccta aacgaacgcg gttgattgtt ttctgtctag
    12421 ttgcagtgtt agcagccatt gccgtttcgt tagtcggacc tgtttcattt attgggctgt
    12481 tggttccgcc agttgttcgc ggaatggttg gaacggacta tcgttatgtt ctacctgtct
    12541 cttttttagt tggcggtgga ttggttatgt tggcggattt tacagcgagg atgatcaatc
    12601 ccccttttga aacacctttt ggattagtca ttgcattgat tggcgttccg tttttactga
    12661 tacaagtaag gagggaacaa cggtgaaaaa aatatattgg acgttctatt tcttgctttt
    12721 tgtacttgtc atcctgtcta taggtagttt aatgattggt acgcctttta tttacctgga
    12781 tcagcttttt gccacgttca tagggaaaga atcatcttct ttggcgctga ttgttagaga
    12841 atttcgagtt cctcgattac tgatcagttt attagcagga gcttgtcttg gcgtttctgg
    12901 ttatatcttg caaggagtaa caagaaatga attggcagat tctagtatat tgggcattaa
    12961 tgccggagca ggatttttag tgatgcttta tttaggtttc ttttcgcact attcgctgcc
    13021 gtttctattg ctagttgtag cctttctagg tggtgtttta gctgcttgct gtgtctatct
    13081 tgcggcctat gatcgaaatg gctttttggg tatgaatcga atcttattgt ccggtattgc
    13141 cgttaatgcc ggcctttctg cattaacatt gctatgcacg atccaattat ctaaggaaaa
    13201 ttatggattt gtgaatgcat ggttagcggg atcaatctgg ggtgcgagct ggccttatgt
    13261 ttgggcattg cttccatggg cggttatctt aattccgcta ggaggacttt atgcacagag
    13321 gattggattg ctatcatttg gtcaagaacg cgcgcaagga ttaggcctta acgtggctaa
    13381 aagtcaaaaa atccttttgt tgttagctgt tgctttggca agcggtagtg tagcggtgac
    13441 aggtagttta tcttttgtag gactactagc tcctcatgct gcgaagctag tcgtacgtaa
    13501 agaaaataac tggacctttg ttctgagcgg tttgttcggt tccatttttc tgcttagtgc
    13561 agatatggtt gctagagtga ttctaccaag tggggaagta cctacaggaa ttttgattgc
    13621 cctttttggt gcgccatatt ttctgtatgc actttttatc agacaaaaac acgcgctgaa
    13681 cagttgaaat ttattaaaaa aggaaggcca atcatttcta ggtcttcttt ttattctatt
    13741 tatgcattta tttgaaagtt cgattgctat ttttcttttt ttcctatacc attttatgag
    13801 agaaggaagg gtgagttttt ggaccaattg aaagtaagat tacgccggaa aggaatcgaa
    13861 aagctgaaaa atctggccaa aagtggcaag aaaaaggaaa aagaacagcg aatctataaa
    13921 cagctattcg ccagcgagta ctggaagtca gcaaaagtga tagggacgac tttatcgaat
    13981 gaatttgaac tggatacaca gccggttatc caacgagcag aaaaagaaca aaagaaaata
    14041 gttgtaccta aaactttgcc gaaacgtcag atggcgtttt atgaaatgga tgaacagtcc
    14101 gttcttgtac gttcgagttt tggtgttctt gagccacaat cagagaagct ctatttgccg
    14161 gagatgatcg atctcctcat tgtaccaggt attatctaca atagagccgg ttatcgaatc
    14221 ggttttggcg gtggttatta cgaccggtat ttaaataatt ttcataaaaa aacatgtagc
    14281 cttgtttttc gtgaacaatt gaatgaacaa tggcaaccgg agagctttga tcagaagatc
    14341 caacaattgt ttattgacta aaatgaaatg ggaggttttc atgaattatc aacagcaaat
    14401 aaaattgcgt gcatggttaa gacgaccgtt atggacctat gcttttttag ggatacagac
    14461 agcggtcttc ctcatgatgg aattatttcc gcgacttgag atcccttatt atgccggaat
    14521 gtatggtcct tatcttgttc acttcaatga atggtggcgt ttagtgacac caatcttcat
    14581 ccattttgga ctgatgcatt tcgtaatgaa ctcactgata ctgtatttta tggggcagca
    14641 aatcgaggca atctatggcc attggcgttt tttcttgatt tatatgttca gcgggataat
    14701 gggaaataca gcaagctttg cttttaacga agcgaatgtt ctgtctggtg gtgcgagtac
    14761 ttcgatattc ggtttgttcg gtgcgttgtt tatcttaggg tttcatttta aacataaccc
    14821 agcgatccag cagctagtca gacattttct tttatttatt gtgttgacat ttgtttttgg
    14881 tcttctggat acctctgtag atgtttgggg acatgtcggt gggctaattg gcggattagt
    14941 gttagggaat gttttgggtc taccgaaaca acagacaagc tattcgatac accaaaggat
    15001 tctttctaca ctagtttttg tatttctttt tgtaatatgt attttactgg gtttaaaaaa
    15061 atatggttta cttgtataat atgaaggaag tgcttaaaga tggagagctt atatgatgta
    15121 cagcagctgt tcaaaaaatt tggcatcttt atctatgtgg gtgcacgcat ctatgacatt
    15181 gaattaatga ctatcgaatt gcgtaatctg tatgatagcc gtttgattga tcatgataca
    15241 tacatgacag cgtggcgcat attaaaaaga gagcaccgaa ttgaagaaag ccgaaagaaa
    15301 ggaaatttgt aatggacaag aagattattg ggattgattt aggtggaaca acagcaaaat
    15361 ttgcaatttt aacaccagaa ggagagattc aacaaaaatg gagcatcgat accaatattt
    15421 tggatgatgg taagcatatc gttcctgaaa tcattgaatc aatcaaccat cgtttgaatt
    15481 tatataatat gaaagccgaa gactttatcg gaatcggcat gggtacccca ggaagcgtgg
    15541 acagcgaagc tggaacggtt attggtgcgt ataacttaaa ctggacagaa gttcaatttg
    15601 tgaaaaaact gatcgaggca ggaacaggaa tcaaattcgc tatcgataat gatgcgaatg
    15661 ttgctgcttt aggtgaacga tggaaaggtg ctggagaaaa cgatccagac gttgtattcg
    15721 ttactctagg aacaggtgtt ggcggtggta tcgttgctgg cggaaatttg attcacggcg
    15781 tgagcggagc aggcggagag atcggccata tcactgtgga tcctgaaggg ttcgaatgta
    15841 cttgcggtaa acgcggatgt ttagaaactg tgtctagcgc tactggtgtg gtacgtctag
    15901 gtcgttactt ggcagaagaa tacgcaggag attcaaaact gaaagcaatg ttagacaacg
    15961 gtgaagaagt aacaagtaaa gatatctttg aaatggctca agaagacgat ccgtttgcat
    16021 tgatggttgt tgatcgcgta tgcttctact taggtttagc ttgcgccaac ttggggaata
    16081 cattgaatcc gtcaagtatt gtgttaggtg gaggagtatc ggcagcagga gaatttttac
    16141 gtagccgtgt agagaaatat tttaaagaat ttactttccc gcaagtaaga gaatcaacaa
    16201 aaatcaaatt agcagagctt ggtaatgaag ctggtgtcat tggtgctgcc tcattagcct
    16261 tgaaatttgc ttaaaaaaac ggaggcatta catggtttta tgggtaatta atatcatttt
    16321 gctattgatt gtgcttgcaa tcatcttttg ggaagtctat ctacgagtaa tggccaagcg
    16381 ttcagcaacg actttgacag aagaagaatt tcgagaagga atgagaaaag cacaagtaat
    16441 cgatgttcgc gaaaaagatg tgtttgatgc tgggcatatt ttaggtgcaa gaaacatccc
    16501 ttacactgtt ttgaaagatt cattaggatc tattcgaaaa gaccagcctg tctatattta
    16561 cgatcaaaag aaaagcttga gcatccgtac agcaaataaa ttacgtaaag caggttatca
    16621 agagatttat ttacttaagg gtggatatga cggatggtct ggaaaaatca aaagtaaaaa
    16681 agtataaaac agatgattgt atgacagctt taaaaaccga atcaatcatt agattttgac
    16741 caagatcaca tgaacggcgc aatggatagt tgtgccgttc atttttttta ttttgtgcta
    16801 ttatctacat atataagcta agcgaaaagg cagaaagaag gaaacaacta tgatcaaatt
    16861 aaaacgtgtt taccttgatg ctgaaaaaga ggatggctat agaatactcg tggataggtt
    16921 ctggccacga ggaatttcaa aagaaaaagc gcagttagat ctgtggttga aagaaattgc
    16981 acctagtaat gaagcacgca aaacttttga ccatgtacca gaacgatttg cttcatttgc
    17041 aaagaaatac cagcaagaat tgattttcga agaagaaaag aagcaagcta tttttaaact
    17101 cagtcaactt atgaaagaac atccagtcat tacttttgtt tatggtgcaa aaaatgaaca
    17161 tcagaaccat gctgttttat tgaaaaaatt tatcgaaatg gaggcatgca aatgaaaata
    17221 gggatcattg gtgccggacc tagaggatta agtatagcag aaaggattct ccataataat
    17281 caggacagac attctcttga actttttctt ttcgatccca atggtcctgg aggtcgagtt
    17341 tggcggttga atcagccacc ggagttatta atgaattctg tttctcaaca agtgacgctt
    17401 tttacagatg aaacattgtc cagtggagga ttggtaacac agggacccaa cttgtttcaa
    17461 tggagtaaag gacctgctac agattatatc gaacagacgg taaccaaaaa taagcaacta
    17521 attatagaag aaatcaatcg tctggaaaaa gatcagcaat ctactcgatg tttatacgga
    17581 ctctatcaac gctggttttt ctcaaagttg caagaagaat ttccagcttc tattcagttt
    17641 gtttattctt tagtaaaaaa agtcagcaaa agaaaagatg gttatctttt acagacagat
    17701 cagcaaagct attctatcga tcagcttgtt ttagcaagcg gtcactggga aaatgatgag
    17761 acagaagaag aaagagtgtg ggtagactac gcaaagaaaa atgatttact gtatcagctt
    17821 ccttctaatc ctgcagatgt cgatctttcg aagataccgc ctaaagaaaa tgtcttttta
    17881 agaggtctag gcttaagttt ttttgattat gtcggattgt ttacagtatc aagagaaggg
    17941 cgcttcgaga acgaaaaagg aaaacttgtc tatcatccat caggcaagga accgattgta
    18001 tacagtggat caagaagggg gctcccttat taccctcgag gaagaaacca aaagcaaggc
    18061 ggatcaatgg catggccacg gttactaaca aaagaaaacc tggagcaatg gcatcagtcg
    18121 ggatttttga ctggcgaaac attttttgac tatctgaaaa aagacgttga gttgttttat
    18181 tacaagaaaa tcatcgaaga atatcatttg ccgattccaa agaaaaattt tgagcatgat
    18241 tttctctccc tctcttcaga ggagtgtctc ggaaagcatc cagcactaat cccttataga
    18301 tgggattggt cgtatttaga aattcctctt acctatcagg atgagtcctt tgcagaagct
    18361 agccgagcat ttattgaagg gcagataaaa gaagcggaaa aaggcaattg cacaggagca
    18421 tttgcttcaa catttgatgc attaaaagac tggcgtgatc caatccggca ggctattgaa
    18481 tggcacgttt tttctgcaaa ggaatacaaa gaaatactat ggggatggtt tactcatcta
    18541 aatagctttt taacgatcgg tccgccagtc attcggacga aagaattagc agcactgata
    18601 gatgcgggta tttttcattt gttcgaaccg ccatttgata ttcagcaaaa gcacaaccgc
    18661 tatgttattt ctctacaaga taaagaaatc agcagtcgct atttgattga agctcgattg
    18721 ccacaaacaa gcttttctcg ttcaagtaat cctgtcataa aaaatttgaa ggaaaacaaa
    18781 cttgttcgcc ctttttcatt ttcagataat acaggaagtt atcattcaga agctatagat
    18841 gttgagcgag gtacgaatcg cgtgattgat gcttcagggg aaatccagtc tgacttttat
    18901 tgctatggaa tacctgttga aggtgtggat tggctaactg cagcagtagc tagaccttat
    18961 acagatccat ggaatctgag acaggcagat aaaatagcac aacttatatt gactgacaaa
    19021 taaaaatacg catgatgact tatctgttct ttcacaagtc ttcatgcgtg tttttaaata
    19081 attattaacg agaaacgcgt ttgcgcattg cttttttacg gttttcttcg atcattgctt
    19141 ctttttcgtc aattggatcg atcactgttt ttttcacaga agcgaccaaa ccagcaacgg
    19201 cagcggctgt tgccagactt cctactaata ttccagatac aaattttttc attgaatcca
    19261 cctcatttat tatttgttat atctattatg gaacaaaaga gaaaaaaagg ctagttttac
    19321 gtttgtttgt aaacctttgc aaaaaatcct t
//
LOCUS       DASJTC010000056        19334 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.056, whole genome shotgun sequence.
ACCESSION   DASJTC010000056 DASJTC010000000
VERSION     DASJTC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.056"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            117..1952
                     /locus_tag="U4B19_001767"
     CDS             117..1952
                     /locus_tag="U4B19_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEN2077506.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(2012..2728)
                     /locus_tag="U4B19_001768"
     CDS             complement(2012..2728)
                     /locus_tag="U4B19_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN2077507.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(2955..3497)
                     /locus_tag="U4B19_001769"
     CDS             complement(2955..3497)
                     /locus_tag="U4B19_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2077508.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(3502..3729)
                     /locus_tag="U4B19_001770"
     CDS             complement(3502..3729)
                     /locus_tag="U4B19_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEN2077509.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(3808..5688)
                     /locus_tag="U4B19_001771"
     CDS             complement(3808..5688)
                     /locus_tag="U4B19_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN2077510.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(5877..6491)
                     /locus_tag="U4B19_001772"
     CDS             complement(5877..6491)
                     /locus_tag="U4B19_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2077511.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            6633..7193
                     /locus_tag="U4B19_001773"
     CDS             6633..7193
                     /locus_tag="U4B19_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2077512.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(7498..7812)
                     /locus_tag="U4B19_001774"
                     /pseudo
     CDS             complement(7498..7812)
                     /locus_tag="U4B19_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(8066..9701)
                     /locus_tag="U4B19_001775"
                     /pseudo
     CDS             complement(8066..9701)
                     /locus_tag="U4B19_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10140..11009)
                     /locus_tag="U4B19_001776"
     CDS             complement(10140..11009)
                     /locus_tag="U4B19_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2077513.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(11029..12150)
                     /locus_tag="U4B19_001777"
     CDS             complement(11029..12150)
                     /locus_tag="U4B19_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN2077514.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(12152..12547)
                     /locus_tag="U4B19_001778"
     CDS             complement(12152..12547)
                     /locus_tag="U4B19_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2077515.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(12566..13372)
                     /locus_tag="U4B19_001779"
     CDS             complement(12566..13372)
                     /locus_tag="U4B19_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2077516.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(13365..14153)
                     /locus_tag="U4B19_001780"
     CDS             complement(13365..14153)
                     /locus_tag="U4B19_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2077517.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(14167..14643)
                     /locus_tag="U4B19_001781"
     CDS             complement(14167..14643)
                     /locus_tag="U4B19_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2077518.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(14715..17372)
                     /locus_tag="U4B19_001782"
     CDS             complement(14715..17372)
                     /locus_tag="U4B19_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2077519.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKR
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNIRKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(17391..17795)
                     /locus_tag="U4B19_001783"
     CDS             complement(17391..17795)
                     /locus_tag="U4B19_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEN2077520.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(18027..18284)
                     /locus_tag="U4B19_001784"
     CDS             complement(18027..18284)
                     /locus_tag="U4B19_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEN2077521.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(18409..18741)
                     /locus_tag="U4B19_001785"
     CDS             complement(18409..18741)
                     /locus_tag="U4B19_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077522.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT         6050 a         3826 c         2932 g         6526 t
ORIGIN      
        1 acttcgcttt tgattcgatt gaacaattaa ctcagcgttg gaaaaaaaga attcattggc
       61 tttttgttca taaaagttat tttttagttg cagcattttt ttactgcttt accttcgtgc
      121 agattttcgt tttagaacca aaagaaggtt ggaaagaaac tatccaggtt gccttaaaaa
      181 tatttactca gcgacaagca cccatgatcc tgacaatcat cattattatg ggatttttct
      241 tcttactatt gttgatttcc aatcgttttt ggtacgcctt gactgctacc ttgatcatta
      301 acttactgct cactatttcg acagtaatca aaatggaaat gcgggaagag cctgtctttc
      361 cttcagattt aaaaatgctg accggattgt cagaactatt atcgatggtt agcccagtct
      421 tattaatcgt gggcggattg atccttctcc tattattgat tactagtatt attgtacaac
      481 gtagactaca aaaacagtat tccttgaaga ttcattggaa acgtcgattt atcagtatcg
      541 ctgtattatt aggatgtttt tcgggtgtct ttttcattaa tcacaaaaat tcgccttctt
      601 tcttgctgtt taatttattt aaagtaaaca agactttctt taaccagcaa ggcgccgtaa
      661 aagataacgg accagtcatt caattcctaa acaatatcga cattaaaatc atggaagaac
      721 ctgctggcta ttcgaaagaa aaaattaatg gaataatgaa aaagtacaac acaaaggcag
      781 atgaaatcaa ccaaacaaga agtgactggt taaacaatca gacgatcgtt ctcaatttaa
      841 gtgaaagctt cagtgatcct gcacgtgtcc ctaatctgac agtacctaca gaccctattc
      901 cttacatcaa aagcattata acagaaggaa caggaggatt gatgttatcc gtcggttatg
      961 gtggcggaac agctaacatg gaatgggaag gactgacagg cttgagtatc agtaacctgt
     1021 ctgcttcgtt agttacacct tatacccagc tagtggagcg tcagaaaata agtccaaata
     1081 ttaccgatct attcgatgaa aaaatagcca tccatccttt tacagctgct ctttatcgca
     1141 gaaaacaagt gtttgataaa tttggttttg acaggttcta ttatttagac agtccggata
     1201 aattaactta tacggataaa atagaacaaa gcccacgcat atcggatgat tcagcataca
     1261 aagaaacctt gaaagcttta caaagcaata cggaaacgtc acaattcatc caattatcca
     1321 ctatgcaaaa ccatatgcct tatgatgatt actatagtga attgaactac actgctgaag
     1381 gaactgctgt cattcctaat agaagaaaag aattacaaac ttatatgcaa ggccttcact
     1441 ataccgatac agcagtaaaa aaattcatcc aagaaatcga caaaatcgat aaaccgatta
     1501 cctttgtttt ttatggtgac catttacccg caatctatag tgggaatgat tcgaaaaaat
     1561 atggattaga acagcaccag acagattatt tcatttataa caacaagtat agtcgtgaac
     1621 aagcagctac gttaaataaa gaagttgttg ctcctaataa ctttggtgct atggctcttg
     1681 agcatgcgaa tatcaaagta actccttact atgcgttgtt gacagaagta tctaatcatc
     1741 tacctgcatt aacaattgat cctacagaaa gcttgtctaa tcgttttaat ggaaaacagg
     1801 tctttgttac agaggatagt caaattatcc aagaaaaaaa tttaactgag aaacaaaaag
     1861 aaattctaga agattatcgt ctgatacaat atgatcttgt tgcaggtaac cagtacagtg
     1921 caacatgggc gacacaaaaa ataaaagact aacaaataaa ctgagcaagg ggttgtgata
     1981 aaagacatgt cacaacccct tgtttatctc attaatttgc ttgtccataa acgatttgat
     2041 taaagccatt tttcaattca ttattcgctg tctcgttgaa gtaaagataa ttatcgggat
     2101 tcccctgatt ttgttgtgtc accaatactt ttggctgatt ttgctgaaac ccgaatagat
     2161 agacgtgctt tttcagatat ggttctgtct ctgcatcgga atagacggct gtgatttgat
     2221 agtctgcttg acctgtccca ctttctgacc attctgcaga aacaggcact ccaccaatag
     2281 ccatcttcca ctccccgttc aaaatagctt gagggacttt aagaccatat aagtccacac
     2341 tcatttgatt cgtataactt ttgtattgtt gtcccattgt ttggccccat ttggtcataa
     2401 atgcctctaa ttgtgcggat ttgtctgcat tccacagtgt ttgttcaact tgttcgctgc
     2461 tttctgaaga ggaagatgaa gcctctacag aagaacttga agtagatgtt tgagttgttt
     2521 cagtcgtttt ggaagactct gacgaagtag aggtatctgc tgtttctgta cttgctgcag
     2581 ttgacttgct ctctgtgcta gttgttgtag tagttgcaac tgttgctttc gctgtttctt
     2641 ttgttgattc ttttttcgat tcattcccgt tggtacaacc agctaacaaa aaagctagac
     2701 caattgttac taaaatcctt tttttcaatt aaaaaactcc tttcgattac tcccacttct
     2761 ttattctatc aaataagcaa tgtgatttta ttgatttttc gtattttctc atcaatcttt
     2821 caaatcttct aaaaactgag aagcacccgc tgatgttgtc tgtaatacat cagcgggtgc
     2881 ttcttttatt ttaatcttcg tgattttcca atttacctca cgctttatat ttgtatttga
     2941 agaaacatag acttttagtc atcatcgtct tcctcatcca aatcttgtcg gacagttttt
     3001 ccttggagtc tttgtacttg gtagatgatt tctttattga aacttaaaag ttcaacaagc
     3061 aattgttgca agccatattt ttcttctttt tcagctaatt tgattgcccg tgtgataaag
     3121 acattttgga aatcaaaatc ttttgcaaag ttttccaaca ttgttgaggc tggtaaatat
     3181 ttgttttgac catgttcgta aatttttcca taacgcatga cttcttctaa agtagacgga
     3241 acgatttcat tttcaatcac taactcatga gagattttat tgaaccatct cgcttcttga
     3301 tcagcatatt ctgaaaaaag ggtacttaat tcttgcgaag atgtacccaa aacatacaat
     3361 tgtgcttgga tcaatttcat ttcttctaat ttaaaatttg ctaatacatg ccccaccatg
     3421 gcgccagcag ttggtttatg atggtcgata tctgcttgtt ttgtttcttg aacaaatttt
     3481 tcttccacta tattcattga tttacgcctc ttttactttt gctgattgga cttcataacc
     3541 taatttttcc acaactgcag ctaattgttc agggtggact tcatctggat caaagtttgc
     3601 tttgattttg ctcgcattaa acaaaacttt cgtattgctt accccaggct gttgagaaac
     3661 cgcatgttcg attttttgca tacagcttgg acaagctagt gtatctaatt gcataataat
     3721 ttttttcatc gtagttcttc cttccttttt cttgatagtc ttattctaga ttacagcttc
     3781 cataaaaaaa ttgattctta tcaagtttta cgatttttct tctttgcttt cgtgtccaaa
     3841 atacaataaa cgcatcgcgt taaagataac gatcaagata ctagcttcgt gaacgaacat
     3901 cccactagcc atgtaaataa acccggcaaa taagccgttg agtaaaaata agacgacacc
     3961 aatggcaatc actacatttt cacgggtatt taaaacagtt ttcttagcca agcggtaggc
     4021 atgaaccaac gattcaaaat tagattgcat caagacaacg tcactcgttt cgatagcaac
     4081 atccgttcct gatcccatag caatcccgat atcagccgca gccaaagagg gagaatcatt
     4141 gattccatct ccaataaaag caacgcgcaa tccttgttct tggtattttt tcacaaattg
     4201 aaccttttcg tctggtaata attcagcatg gacttcatca atgcctaaca tatcagctac
     4261 ataatcagca gtcatctgat tatcacctgt cagcatcact aactgttttg ctcctgcttg
     4321 tttcaactta gctaattgtt cagcgacttc tggtcgaatc acatctgaca cacctatgat
     4381 ttgagtgatt tttttgtcga ttgctacaat aatgaccgag ctgcctgttt gttgtaattg
     4441 atgtaaatct tgtacttgct ttggtgtgag agaaagtttg tgagcggcaa tcaatttggt
     4501 gttgcccgca caaactgtat gaccatcaat ctcagctgtg attccttgtc ccttgattgt
     4561 ctgattatct ttgactgcta attgttgaaa atcaatgtct ttctcctgag cataatggat
     4621 cacggcgcgc cccagaggat gatcggataa cgtttctaat cgagccacta aagctaagct
     4681 gtccccttca tcctctgtat agttcttgat agtggataca gcagtagttc ctttggtcaa
     4741 tgtaccagtt ttatcaaaga caaaagtatc gattttggaa aaagtatcca ttgcttcacc
     4801 gcctttgact aagatgcccc gtttcgctcc attaccgatt cctgctacat tcgaaacagg
     4861 cgcaccaatg actaaagcgc caggacaacc aagaaccaaa acagtgattg ctaaacgaaa
     4921 atcacgacta atcagaccaa caaccaacgc aataagtaaa acggttggag tataatactg
     4981 agaaaatcga tcaatgaatt tttccgcatg ggatttggta tcttgtgctt cttctactaa
     5041 ttcgataatt ttggcaaagg tcgtatcatc tccgacctta gttgcttcaa tcgtcacgta
     5101 gccatctgct aaaatcgttc cagagaagac ttcatctcca ctgattttga cgacttgtcg
     5161 tgattcacca gtcactgcgg cttcatccgc ataccctttc ccgtcaatga ctcgtccgtc
     5221 cacaggaata gctccaccag tttttactaa gacgatatcg ccttcttcca cgtcatcgat
     5281 ttccacttct tcagtcgttc catcttcttg gacaacacta gcagtagatg gggccatctc
     5341 ggtcaaagcc ttgattgact cccgcgtttt ggtcaatgtt ttttgttcca gatagctacc
     5401 aaacaaaaac aagaatgtaa cgatagctga ctccttgtat tctccgatca taaaggcacc
     5461 aagcacagca atagtaacga gtaaatcaat tgagataact ttgactttca ctgcctgata
     5521 cgcatggatc ataataggta tgaccgcaat aatagaagct atggctagag caatgttata
     5581 gaacaagaca aaaccagtaa attttccgat aaaaccaaaa ataatcaaca cagctgataa
     5641 ggcggtgata tgatttcgat gctcttggat ccacttttgt aatttcatct ttctccctct
     5701 tttcctttca ttcttacgag actaagtata gatacttatc catcataaaa aattgaccac
     5761 tatcaagttt agtcaaaaag gcggttttct tttggtagaa tacactacaa aaaatagtta
     5821 gcaagaagac tcactaaaag tgatcttgcc aactattgtc gtttctatcg aaattttcat
     5881 tgaatgattt tcctgtacag catacatcgg agatctattt ctttatctaa gatttttatc
     5941 ttgctaattt tttcctttag tctttcaaat ccacgtcgtt catagaattg atacgtacag
     6001 ccactatctg taaaaaggaa tatttctttt cctttctctc ttttttcaaa ttctgttagc
     6061 aacttggtac cgatgccttt tgttttcaaa tcaggatttg cagctaaaaa aatgatctct
     6121 ccatctggcg aatgcttctt taaatatgct gcaagcattt gttcatttgc tgtctcgtaa
     6181 gctcctttac tttctttagc aaatatattt tgtaaaaaat cgaatatctt aacatatatt
     6241 tgctgtccaa aagaacgatg tttctttttt tctcctttta tctccgccag caaaacacct
     6301 gccaactcgt cctctgcata caaagcaaga atttgagttg ctcgattcaa ctccatatac
     6361 aaaaaatatc ttccgtaaag attcagcaga agttcatttt ccatatatct ctcaaagttc
     6421 attccttcaa cagcaaaata gatagctttt ttgtaatctt tcttttctaa gggtttaaaa
     6481 gttagtttca tcatttcgcc tcctcaactt caatgattgt gttatactta cattataata
     6541 gacacagtgt ctactatcta taatcaatca tctataagtt tgtcgattaa gcgacagctt
     6601 tgctttgata tgtcggataa ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa
     6661 aaacagaaaa agctttggct gaaacactat ccacattatt agtaaataaa aagattcaag
     6721 caatcactat acgcgaacta acagaaacgg cagatgtaca tcgttcgact ttttatacac
     6781 attataagga tatctatgat ctatatgatc aattagaaag caactttttt cgggatctaa
     6841 atgaaattct ctcttatgat ccagcccata gctatgagga actatacact cgcttgattg
     6901 actatgttta cgcaaatact tttttatatc gcttatttat cctcaaccat ggtgatattt
     6961 cgtttcagaa gaaagttaaa caagttattg aagaaaacta tctgaacata tggctatatg
     7021 aagacaagaa aacgattatc acagaagaaa tgagattctt tactacgtac cacgtccaag
     7081 gttgtctatc cgttctcaat cggtgggtag aaagcaatta caagtactcc aaaaaagaat
     7141 tattagaact cttacagaaa ctgaatgatc acatagagaa aatcatgcct tgaataaaaa
     7201 catactaagc ttagttaaga agaaccgtaa ccttctcgta taacaactga atcttattta
     7261 gccaaattgt ttactgaaga ttttttgagt aaaatttcta taattcctac ttattgaaaa
     7321 gtagtttgta gtgctgcttc tccctctcta agtgatatat cttgttgtac caatcaaatt
     7381 cacctcatca ttaataaatt acttttaggc taatttatta caacttatag gttgaagctg
     7441 taaccaattt atagtcatat tatattttta tcatccagtt tattgaattc ttctcgttta
     7501 cctcaattca ttttcaaggg gaatcagctc atcatctaac attttacgcc atgctcgctt
     7561 catttcttgt gggtatttaa atggtttcgc ttctggatgt ttggctttat atccttcgta
     7621 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
     7681 cagattagaa gcatgtaatt ttttctcaga aacaaaccac tcattggaaa agacatgaat
     7741 caaatcatca cttacttgtg taaaatagcg acgtttaatg acaaagatat cttcattgct
     7801 atctagtgta ccttcatttc tttgtgctta aattcaaaga taattttctc aataaagatt
     7861 tttgattcca gtaagattcc tttactcctt ttacaacata gcgttgtcct ttgttttgaa
     7921 acatcttctt cccctctttg ccattaaaaa tagcccaatt ctctataaag aagattaagc
     7981 ttcattatta taatataaag tttaaatttg gtttattact tactttttct tattctctag
     8041 taagctaata ttgaaaaaaa tgtaatcaga tcattatttt tatatgttgt aacttcctga
     8101 taagaaaact aattaaaaga aagaggtaac tacataaact aattaaaaaa gctaacttca
     8161 tagttggagg ataaatggaa gaaacttcaa tgattgtttc tccttgtgtt ccttcaatcg
     8221 acactagtcc tattatcaat ctctcttttt tgtccattta cttttgcgac tgttccccaa
     8281 taatcaaata caggaagcgt tattttttgt cctttagatt catgtactgt atagactatt
     8341 ttcgaatcca tcacttgata aggcatagta tgccattcct tgccaattct tacttcatga
     8401 ttctgcaaat gttctttgta ttgtaatgct tccgtgggtg catagtcata aagtcctccg
     8461 ggaaggagtg tcgtgtaacg aataagattt tcttgattca ttaccaagtg atctctctga
     8521 gtgacaactt tatgttcata gtttgctttt gttgcagcag atacagccaa cagtaataaa
     8581 acaattgccg ttatattaac tacttttctt tgcttagctg atgtgctctt catgaaaacg
     8641 aacgataaaa tcaacgagcc aaacagtact tgaaagccta agattcgcca aggaaattga
     8701 atcatttgaa tgggtgtatt ttgtaataaa gaccatggaa aaaatttggt ggataagagc
     8761 caagagacta ccgcactaag gtaaatcact ttttctttgc gattaaatcc tttaaaaaag
     8821 aagagtggac agatcaaaga aataatacca atgattccta ccgtaaaggt tgtcaggtca
     8881 ttattcatcg caccaatcaa actatccgag acatttaatg cttggtgttg taaggtcgtt
     8941 ttatctggct cattaatcga ttgaaccatg atttgtcgga acatcggata ccaaaagtat
     9001 gctgttaaaa gaatcgtcat tcctactgcc tttgacaact ccacaatcaa ccggatgctt
     9061 tctttttttc ttgtttcact tactacaaag ccgcctaaga ctagtaagcc aacggctatt
     9121 acactcataa aagtggaaag aacatgtgta taaatgagta agctcatact gactgctaat
     9181 ggaattgcac atggcttttt ctcataaatc agtgcgtaag cataatacat aactatcggt
     9241 ataaccgtaa gtgcaatcgc ttcagcaata gcagaacgat gataaatatc aatcgtacgg
     9301 tacccataaa atgtatataa acagctaaat ataaaagccg catcgattct ttttaaaaat
     9361 tttagtccat aatgaaacat cagcatgata gtacaaacat tcaatagcca aacatataag
     9421 atataactaa cgattaaatt attacttatc caataaaaaa tgactgccgg aaaaaaggtc
     9481 aagtagggat aaaaataatt cactccgctc ccataatgat taaacgtcgt aaaattgatt
     9541 ggaccagaaa aaattgaaga taaccctttg actctagcaa aatggaaggc aatatccccc
     9601 gatgtagaaa tagtatgaac atttctaaaa taattgattg aataaagtcc agcaataatt
     9661 gctaggagaa tggacaatat tatttttttc tttacttgca ttacttaatt atatactcct
     9721 ttcatggatt gctttatcta gtataactta aattttaaat aaatcgttat ttatttttac
     9781 tctcacttaa aaaagcacta aaattataag agagggaggt aatggtctga acaccaagaa
     9841 ttgttccaac atcgaattgc ttaactaatg agttcaaagc ttaattatgt ggctcttata
     9901 tttaccgagc gtggattgcg atctctttag cgaaacaaac atctttcata aaaaaaacag
     9961 ccatatccgc tttcttggat atgactattt actactactt tactatcttt cattcttctt
    10021 gtctcactta tgataaggcg ctgggtatca tgagtgaatg atattgctga atacaaatag
    10081 tccgaccaca ttaagttgat cggacttcat aagcatttta ttgactaata cttttttgct
    10141 taagaattga ttctaacatc ttttgctaaa gttaagcatc ctatagttcc tgcattatct
    10201 gctaacttag gtgtgacaat gtattcttcg atatttggcg tatcgacata gccgttaatt
    10261 agttctaaaa aacgtctttg tactttcttt ttcagatgtt cttgttgcat gacaccacca
    10321 ccaaaaatta taatatctgg tgaaagcatc agggtcgtat tacaggcaca ttgtgcaata
    10381 taatccgcct cgatttccca gaacggatct tctatagaaa gatcttgtcc cttggttccc
    10441 gtacgcatct cgatagcggg tccagatgcc aacccttcga gacaatcatg atgaaaagga
    10501 caagtaccag agaaagtatc tcctgaatgt ctcttaacta acgtatgccc catttctgga
    10561 tggctaaacc cttcaacaaa aatcccatct tgtatagctc ctccaccaat tccagttcca
    10621 acagtgtaat aaacaacact ggataaacct tttccatgac ctgaaacata ctccccgtaa
    10681 caagcagcat tcacatcggt tgtccaggca attggaattt cgaaatgctt attcaacgtt
    10741 ccaacaaaat caaaattttt ccaggcagtt tttggagtag aggtgatatg tccataggtc
    10801 tttgattcac gatggatatc aatcggtcca aatgacccta ctccaatgct ttctaattgt
    10861 tctttatagt gattaaagaa atcaattacc aaactcattg tttcattcgg tgtagttgta
    10921 ggaaaactca ctctttctaa aacctttaaa tcatcattgc caatggcaca aacaaattta
    10981 gtcccacctg cttctattga gccatacatt tcttactcca ccgtcctttt atctaggaat
    11041 tcgttcttcc ctgtctaata gtttttcgaa aaactcatgt ggttcctttt ggtaccaata
    11101 ggcaacagaa gaaatatcgt cttgacgctc ataaagcttg atgtcatcat ttcctagggt
    11161 ttggaaagta acacgtaaat tttctttgaa actaatgggg tcgggtaaat gccagcggta
    11221 caacccatgc atcggtaaag catcatcgcc aaaaccatga actggatttg gattaggttt
    11281 tccagcacta aaataatccc gggtgtgatc tcttttagat tgaaaaggat agcccataaa
    11341 tagcgtatta aaattttgag catatgccct ttctccaaag ttctttttat ggaaagccca
    11401 agcgccgcca aaataatctt ctgctcctgt agatgttatt gtagggaaat cttcatctcc
    11461 atctatataa aacttaaatt ctccttcccc ccaccagtat ctttccaatg cgcacaaagc
    11521 aaagtaggtt cctacataat acccagtgcc tttgattcca tcaataaggg tgtaatcttt
    11581 tccttttgtc gtgatgggtt cacggttcca tttagcatga aaatacatcg catcatccgg
    11641 tacttcagtc aatgcataat taaatgtata aaaaatgtgc ttaataaact caggatgttc
    11701 attcgttaaa gtaatcttag ctttttttcg aaatggcatc tcaaaatagc aattcatccc
    11761 tgatgttgga ttcactacga ttggtaagga attcacttcg catcttgtcc caaacccatt
    11821 acaaaaaaaa tctcctaatg gactttctac agaaggagtg gattcatcat cccaataaat
    11881 acgcaaaatt aaatcacgta agacgaaact cccagcgatt gttttgtctg tcactgtcat
    11941 ccaaaaatga cgtaaaattc cagggccttc catttctgct aaagtaattg tctctccagc
    12001 atttatcgga acagaaggtg acccttttct cccaattcct agatgactcg ctgtcattgc
    12061 cgctttccct ttttcaccat ttttattttc tggtgaaata gttctacttt cctctccctt
    12121 gtggagccat atattattta aacaattcat tttattcgtc ctctatcctt tcttcacttt
    12181 tttgattatt catatagatg atttgttgtt gtgcctccgc aataattgct ccaatatcta
    12241 aattatcatt tcctaactgc aaagcgattg acattgccaa gggaagattc attcctgcaa
    12301 ttacgtgcac tctgtcagaa atataggtaa aaaacttttg atttacactt cctccagcca
    12361 tgtcagttaa aacaattatt tcgtctgtag tatccactaa ctctttaatt tgttcatcta
    12421 tatcatcaat ttcttcgaca tatgcacata agctactaat ttgattatgc atacctgtta
    12481 aaaactctag tgtctcttta aaaccctcag ctagtattga atggctagca atgatcaatc
    12541 ttctcataat tcaaatcctt ttcctttacg ctaatattcc aaatgcagcc ataaccatcg
    12601 caaaaacaat aacgagtaga atcaaaatgg tcatactgat ttttttctta ctgagtagat
    12661 aatatgctgc agcagtaata attactggaa ttaatgttgg taaaattttg tccaacattg
    12721 gttggatttt catactaact tcaccaaaag tgaatttaag tggcgttgaa acactgataa
    12781 ctgaagcaat taatgatcca actactgtta gccccaatac tgaagcagct tcagttaaat
    12841 aattaatctt ttcaccgtat tcagtaacaa tcttcattcc ctgcttataa ccaaattcaa
    12901 agcctctcat tcggaaaaag aagattacca tgattaaaat gaaccataaa aagattccta
    12961 caggattatt ttctaaagcc atataagccg caattgatcc catgatggtt gggatcatcg
    13021 tcatcaaaag agagtcccca ataccagcaa ttggacccat cgtaccaact ttaaagtcat
    13081 taatcgcatc ttttgcttct aatccttgct tttcctccat tgcaacacaa gcacctaaaa
    13141 taatggctgc cagccaaggc tgtgtattgt aatactgaaa gtgattattt aaagcttcga
    13201 tgtattcatc atcatctttg tataattttc ttaaaatagg tgacaatgcg taagtcacag
    13261 atgcaccttg ctgtgtttga taattaaagg tacaaactga catcaaccaa cgatagttcg
    13321 ctttcctaag atcctttttc gttacttgat atttttcgct cattttattc atcctcccca
    13381 ataaacccaa catcagaagc tcccgcagaa gcagtgattt gtgcatcttt ttcagctcta
    13441 tgatacacat taaatgctaa agcgaatcct atgatcgccg ctccaataat aggtaccttc
    13501 aaaaaagcgg ctaagaaaaa tccaacaagt aaataactaa tgtatcgacc tgtaggcata
    13561 aagcgtaaca acatcgcaat accaacagca ggcaacattt ttccggctaa tgttagccca
    13621 tccataaacc aattaggtaa aacatccaaa acgtaattaa cagcagactg tcctaaagta
    13681 acggcaataa atactggaat tgctgcagat aaaccaaaga aaattggccc caaccacaaa
    13741 acactattca ttttcttaaa ttctcgttta ttcgcatatt cctgtgattt gcgaacaaca
    13801 aaaccattta agattttgac aattacatct agttgcaccc ctaacatacc tacaggtagt
    13861 cctattgcta atccggcttc tactccaagc tggctaccaa atgccgttgc aataattgcg
    13921 cacgttccat agtccggtac agatgagcca ccaaaaccgg caactcctaa tgacatcaat
    13981 tgaactgtac cgccaactat caaacctgtt tttatatcac ccatcaaaat tcctgtgaat
    14041 aatccccaaa aaactgcgta atttacaaag atcatcggaa tcccataata atcaacatgt
    14101 ttaataaatg ctaatatagt cagcataata atttgaaaaa tagtaaaatg cattatcctt
    14161 actcctttat cctaattttg attgaatttc ctgtgcctta tccgccggaa caaattgtgt
    14221 aacaatttct acaccagaat ccaacaattt tttatacgtg ctgatatttt catctgatgc
    14281 ataggctcta gaagatagct gtaatgcatt atctttaaca acagtaccgc caacaattag
    14341 tcggtcaatt ctaataccgt tatctactaa atgaaggatt gtttccggct ctttcacgat
    14401 gacaaatact ttttcctgat cgtatttccc ctttttaaaa ttcgcaatcg ctgtttcttc
    14461 agtgataatt gatacagcca ttccagcagg tctagctaaa cgcatacttg atttttggat
    14521 gtcgttattt gcgacagaat catcgatcac catcactctg tttgctttag acattggtgc
    14581 ccattgcgtg acaataatcc catgcaataa acggttatca actctcgaca taacaatact
    14641 cataaatact tcctccttta tctttcattt tcttacaagc aattaccatg ccaaaacaaa
    14701 aagaacttga taaatcaagc tctttttaca taatcataaa cgtatttaac ctcatgaatc
    14761 gggatgtcga cactataata atgttcaacg acactgaaag attgcttaaa atgtgtctga
    14821 aatttttgaa attcctcact cgtttcattg aaataaattt catcttccaa agtattttgt
    14881 tttactaatc gttcaattaa gcaactaatg tgaacatata atccgaaaca cgtattatta
    14941 tcaaatgaaa ttcctagatc attttgtaag atgtatatag catctgaaac ccgttctaat
    15001 aatttagtcg gatttaatat ggtcaattgc tccattaaat tcgttagact aaaattcttc
    15061 agaatattat tgttcaaaac tttcagttca ttatcattaa agtgtctatt caattgattg
    15121 gagaattttc ttatattcat tcctaaaatc atatcttcaa ttgaaaagaa ttctacatct
    15181 tctagatgtg gatttagtgt tccaacaata aaaagaatat tgttctcttt acgaagctcc
    15241 gataaatagt tttcattcac taaagagtaa tagtcacacg tttctatgac aagatcagta
    15301 tcccttggaa agctatcttc aagtatcctt tttaatttaa acgaagttcc taatccgctt
    15361 gcacaagaac agacaatcat atccttctta atatttgatg attcataaat ataatccaca
    15421 ttttgatttt cttttacttg ttcaagaatc tgtttaagcg gtaaatctaa taatatctga
    15481 tttgcaacat caatagccat tcttgttgaa acattgttta gtagtccgaa ttttttttga
    15541 gcgtctagtt ccaaatttag ataaattttc tccagtgaac ccatatcaac aagtaaaata
    15601 atttcttcat atccggtaca ctcttgtaag tatctattaa ttttgtctgc aatctcacta
    15661 cttgaagtca ttagtggcat atcaatggcg tcgtatatat atctctcaac gatcctatta
    15721 gcagtacttg ctatacaggt tgccgttaga tctccatgag ccaaaatcag tgctaaacgt
    15781 ttttgctgaa attctttact acacattaac ttaaataaaa taaccaaaac atttttttga
    15841 atttttgtta aataaatatc ccctatttga ataaaacgat tgactaattg aacttctcga
    15901 ctacaccaac tattaatatc attgatgaag gaatataatt tatcgtccga tgttaattgg
    15961 atttgataaa tccaagaatt caaaaagata gacgttattt gaagataata atagtcaaat
    16021 tctatttgaa aatctctctc tatttcctta acatactgtc taaatcgaat ctcttgatag
    16081 gtgggatatg ttaaagctga attattttgg atatcaacat tcttttgtct aaaatatcta
    16141 tcatatttct caaaaagctg ctgcttatca gtgtctttct tccacgtttg ataaagatac
    16201 tctataagtt cgttgatatt aaaaacttcg tctacttttt ccaaatcatc catttgaata
    16261 tattttaatt cattctgtct tttcataacc ttcatgccgt tgaaattcac ccattgtggt
    16321 aaatgataca tctgaatata aagttcttcc aaatcatcta agaaataaaa ggcattggca
    16381 caggtcaatt gaatgatatt tttcaaattg ccaacatttt cactaaagcg atagtttaat
    16441 aaaaaatcta ataattgttg agatatcagt attttctttg ctagtctttc ttcttctgat
    16501 ttaaaaaata tcttgattaa ctgttttctt tcataaagag aacgactgtc taatgttgga
    16561 agtgttagct ttacaggtat tcttctcaac aaagttgata atagtttatc ctcaggtggt
    16621 tctgatgttg caaatataat ccagcaactt gatgtgtacc agttttcatt atcacccatc
    16681 cgatgaaatt tcctcgtatc cataaactga aaaagtttct cctgacattc attgctcaat
    16741 gcatgaattt catctaaaaa taagactcca ttattagcta attctgctaa accctttctt
    16801 ccatcttcgg ctcctgtata tgcacctttt acatgcccaa aaaaattgga caagaaaagt
    16861 tctggattat tggcatattc actacaatta actgaaatga aagatgcctt ctcttctata
    16921 taattattgg ccaacaaatc cttatacaca taatttgcta aatagctttt tccagttcca
    16981 gtctcgccat gaagtaaaat gggtaagcca tttggcggat agtagattgc tgctttaatt
    17041 tgagcaatta catttcgcag acttccttga cttccgataa ccctttctat tccaacccta
    17101 aaagagctat cataccattt ttccgcttcc tctgaagagc taaaaatttg ttgatttgga
    17161 actttctccg gtaaagaaaa gaagattact ggacgagtat tgatttttcc aaattctcca
    17221 tcttttacga attcattcaa atattgagag acaatattac gactaacatt taatttttca
    17281 gatattttct gtgctgtatt gttcccccat ttatttttaa tctcagagtt ctctatgaca
    17341 taattgacaa tgcgttcttt taattcttcc atcacagcac ctcctcgaca ctattcgaat
    17401 tttgccgaca gtatttttcc ataaatatga cttgctaaaa aggctttgtt tagatcattc
    17461 aattggaacg ttgcttgttt tgcttgatcc gctaagacgt tgaaaagata ctctggcgaa
    17521 atcgcatacc ccaaaatatc aacgattgtt gcaatcagat cttcttttga ttcttcatct
    17581 gaaagtaatt cacgatacaa catcgtttgt ttgcttactg tgtcatattc ctctaaggat
    17641 tcatctgcta aaagaaccac ttgttccaac aaacggtcgg ataaatattt atagaaaatc
    17701 agccccaata aataattttt atattctgac gcatccattt tactacggag attatctgcc
    17761 gcactaaata acttctgatt taattctgca cccaaaaaga tacactccta tctcttattt
    17821 aaagttgcta tataccctct tattttagca tactcagttt tagaaaaagc aaaaaaataa
    17881 agaaacttat tcttgataac aagctacgtg acgtatatcc ctaacacaaa aatagccata
    17941 tcctttggtt tggatacggc tatttgatat tactcattca attatcaaat cacagtaggt
    18001 gtgaatctca tcgggatcgg caatatctac ttctgattag gatcaaaggt gattttctca
    18061 ataaaggtgt gatacaaagc tttaacttgc ttcttctcac tctggataag aaagcaatcc
    18121 aaactagtca atatctgttg gacatctttg acttgaatcg gttccccttg ttgttgaaag
    18181 atccctaaga tttcattctc tcgaatttgt aactgtcttc gtttttcagt caattcattt
    18241 aaacgatctt ttaacatcgg aaacaattcg ggactatctt ccaaagcagc ttcctgtttt
    18301 tgctctaata gttagatttt gcgtaccatt ttcttctttt attttccttc gattgctgat
    18361 tttaatatag tctaaaggaa atttatattt attgtattct ttataacttc aagagaactt
    18421 cgtttgagta ttactgtatt tttttaattc cataagaaaa ttagttatta cctgccacct
    18481 aatcagcaaa tctgaacctg agtcaatata attaatatta ttttccatct ccaacattgt
    18541 aagtactgtt ggtgttaata tagggacttt gtacaattgt atttttttga ttttatcatc
    18601 tgcacaaagg agatttttta aatctaatcc ttcttttatt tcaaaagttg aattttttaa
    18661 atctataaga gtaaaaccaa ttccttcata ctgaatttct gaaaatcgaa tttttctccc
    18721 agttgataga tcaaggggca gaaaaccttt tattacattg tgagtgtcac catcatttac
    18781 aatgtcatta ggaattttga ttttagaatt ctgtggagca cctattggaa gataataatt
    18841 cattataatt ttgtagtctt catgaggaaa taaagtactt ttaggattgc taagatattc
    18901 tcgtaccgaa tctaagaatg gcttatccat tgaatcttca tccccattta aactaaaaaa
    18961 cattcctaca attgcttttg caactctaag tgaattcatt ttctttattg aatgtgttaa
    19021 atcagactta ccattcttta aacttgtatt gttataaagg aaatttttat tgtcttctgc
    19081 aaatctaata tattcctcaa catactcact accaaaaaaa ctattacatt tactacataa
    19141 cgtattggaa tggcaacagt ttttttgaca aattttataa ggtgcagaac ttctttccgt
    19201 atgctattcc gattgtcctt gacaatgagc ctcctcggat gtgacgatct tcggcaggag
    19261 ctaacagtta aagttagact gcctcgctag cgttagcaca tccgagctca ttatcaagga
    19321 ttcgctgcgc caat
//
LOCUS       DASJTC010000057        18641 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.057, whole genome shotgun sequence.
ACCESSION   DASJTC010000057 DASJTC010000000
VERSION     DASJTC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.057"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            192..1379
                     /locus_tag="U4B19_001786"
     CDS             192..1379
                     /locus_tag="U4B19_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2077523.1"
                     /translation="MQQLSQNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKK
                     QVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIES
                     VTENSGRFYDKENKEILSIGFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWEN
                     TDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFE
                     SHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWS
                     SQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFD
                     FQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAV
                     QNK"
     gene            complement(1471..1836)
                     /locus_tag="U4B19_001787"
     CDS             complement(1471..1836)
                     /locus_tag="U4B19_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN2077524.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1833..2198)
                     /locus_tag="U4B19_001788"
     CDS             complement(1833..2198)
                     /locus_tag="U4B19_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEN2077525.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2390..2638)
                     /locus_tag="U4B19_001789"
     CDS             complement(2390..2638)
                     /locus_tag="U4B19_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077526.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2637..2765
                     /locus_tag="U4B19_001790"
     CDS             2637..2765
                     /locus_tag="U4B19_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEN2077527.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2822..4516
                     /locus_tag="U4B19_001791"
     CDS             2822..4516
                     /locus_tag="U4B19_001791"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEN2077528.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4520..4639
                     /locus_tag="U4B19_001792"
                     /pseudo
     CDS             4520..4639
                     /locus_tag="U4B19_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4616..4789
                     /locus_tag="U4B19_001793"
     CDS             4616..4789
                     /locus_tag="U4B19_001793"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077529.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5167..6300
                     /locus_tag="U4B19_001794"
     CDS             5167..6300
                     /locus_tag="U4B19_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEN2077530.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6331..6651
                     /locus_tag="U4B19_001795"
     CDS             6331..6651
                     /locus_tag="U4B19_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEN2077531.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6680..7144)
                     /locus_tag="U4B19_001796"
     CDS             complement(6680..7144)
                     /locus_tag="U4B19_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2077532.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7276..8529)
                     /locus_tag="U4B19_001797"
     CDS             complement(7276..8529)
                     /locus_tag="U4B19_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2077533.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8558..8875)
                     /locus_tag="U4B19_001798"
     CDS             complement(8558..8875)
                     /locus_tag="U4B19_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEN2077534.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(8901..9221)
                     /locus_tag="U4B19_001799"
     CDS             complement(8901..9221)
                     /locus_tag="U4B19_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2077535.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9685..11100)
                     /locus_tag="U4B19_001800"
     CDS             complement(9685..11100)
                     /locus_tag="U4B19_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2077536.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11117..12433)
                     /locus_tag="U4B19_001801"
     CDS             complement(11117..12433)
                     /locus_tag="U4B19_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2077537.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12583..13281)
                     /locus_tag="U4B19_001802"
     CDS             complement(12583..13281)
                     /locus_tag="U4B19_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEN2077538.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13570..14526)
                     /locus_tag="U4B19_001803"
     CDS             complement(13570..14526)
                     /locus_tag="U4B19_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEN2077539.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14639..15472)
                     /gene="msrA"
                     /locus_tag="U4B19_001804"
     CDS             complement(14639..15472)
                     /gene="msrA"
                     /locus_tag="U4B19_001804"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEN2077540.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15647..17281)
                     /locus_tag="U4B19_001805"
     CDS             complement(15647..17281)
                     /locus_tag="U4B19_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077541.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17268..18641)
                     /locus_tag="U4B19_001806"
     CDS             complement(17268..18641)
                     /locus_tag="U4B19_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077542.1"
                     /translation="MISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAI
                     ALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFL
                     SKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSIS
                     DSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPW
                     FLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTS
                     IAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKV
                     GESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVN
                     VDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEI
                     NKKNENK"
BASE COUNT         5882 a         3687 c         3119 g         5953 t
ORIGIN      
        1 gtgggcaatt ctatgtaaat aaagaagtca atctagaaaa gctctccaat ctcctgcatg
       61 tttgcaaaac cactttactc aatgatatca ataacataaa aaaagaattt gaagaacaga
      121 tcgcttatac acatcgggaa aaggaccgtt acactttata tttttcagaa catatacctc
      181 gatgcaagat catgcagcaa ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt
      241 atttggaaga agatgaacct gattatcttc aattaactga atgtgaattc atctctgtca
      301 gcaaagctta ttcattaaaa aaacaagtat tagcttattt taatgactgc gggatcgaaa
      361 tcgaccgcta ctcccctcga ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt
      421 cgtatcgttt aggcggtttt aattcttggg agctacctga atcgtttttt gaacgtgccg
      481 atcgtttcat tgaatccgta acggaaaata gtggcagatt ttatgataaa gaaaataaag
      541 aaatcttatc gattggtttt gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa
      601 tagattcaaa atttatcgaa gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag
      661 catgggaaaa cactgatttc caaacctatt ataaaaaaga agaattcgcc tttattttga
      721 cactattcaa tctttgtaat tatggtttcc attcttatca gttgattgcg gaagactttc
      781 aacagctaca ccaagtgttt attgacaata ctcccgaaat aaaagaacta gttgctacct
      841 ttgaatctca cttcaatcaa gaattattcg gaaaccaacc gttcgaacgt gcgctgattc
      901 atctgatgcg ttcggcttgg gataactatc aattattcat gccagaaaaa ttttatcttt
      961 taaatgagga acagacaaat ttatataaag aagtccagac aattttttcc agctggtcca
     1021 gccagttacc ttatgatcta cggttgaatc ctaattgcat gcgggctttt gtgatcgaat
     1081 tgagcggaat tctccgtctg acaaaagaac atctgactat ctatatcgtc acaaattcgg
     1141 atgttcatta tctgatttat cgggaagcct tggaagctgt aactactttc gattttcaag
     1201 ttgcaccaac aatttattca agtatatctg atataaagaa gtatgcgcag cagtcctcaa
     1261 accgggtatt atgtgaacgt acattatata caccagatgc ggtccaatat gaaaatatta
     1321 ttcctatttc gatcaatacg atcgatcgag ctatcatttc agctgtgcaa aacaaataag
     1381 gggctgtgac aaaaatcatg tcacagcctc ttatttgttg aagatgccca tttctgtaac
     1441 aatctcttga tacacgtcag gctcacaacc tcacttattt tggacatcta cgggtccgat
     1501 ctctttattt aaaaaataag aaatgatggt tcggataact accaacgccg ctagttggaa
     1561 aatatcctgc aaggtaggtt tgataatcga ttcgatgatc ccagctgaaa caagaatttc
     1621 caaacttaga agtatatagc ctcccaacat ctttttaata gcattgttct cgctgactcg
     1681 gtcgctttgc acttttgtat ggacaaataa atatttcaaa aaatccttga ttgatagaca
     1741 aacaccccaa accaataaac agatagcaaa gatatcaagt cccagtacaa gtttttcgaa
     1801 atatggaaga agaaactgca tcaactctgt cgtcatccta cctctcctct tctttgtttt
     1861 tctttgtctt ctatttcctg attcaagaaa taagaaatga ctgttcgaat gataataata
     1921 agtgcaagct tcaaaatatc tgtccaagtc ggcttgataa ctgactcgat gatatcagca
     1981 acgactagta cttccaagct taacaaaata taacttgcta gcagttttct tgtatctgca
     2041 ttacgagtat tacgttctgc atagtttttc ttgacatgag accaatcaaa aagtttgaat
     2101 agaacaagaa cgatcccgac aattaagata ccgatagaac aaatatttaa aatagcaatg
     2161 atccattcta gaaattcaat taaatttgag cgaaacatat acgctaccta ctttctttta
     2221 atttgttcct attcttttat tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt
     2281 tctaatgata acggttcgct taaatttttc tttttcactc tcccattttt ctccctccat
     2341 ccataaatcg tggaagtatc caaaacaacg tagcaagtta aaataaggct tagtccgtca
     2401 tcagcttttc aaagaagcac tgtctcggtg ctttttttgt acccaaattt ctaaaaatag
     2461 aaccatacac ctcctcaaat cttttcttct tacctaccct atttttattt acaggaaaat
     2521 cagacacttc ttcttttttg tcaaaatttc ctttttcttc tcgtcctatc atttggcatt
     2581 ctaataaaaa cgaactatcc ttggatatag aaagagaaaa caaggaggcg tttttcatga
     2641 gtaaaaaatt aatcaaagtc ggcatcggtt taggtttgct tgcccttggc gctgcatatc
     2701 taggaaaaaa gacaggacta ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa
     2761 tctagcatcc tatttagttg aatcactacg gacatacttg gaagacattg gaggaatcac
     2821 aatgagaaaa agtaaagcaa taatgattgg tgccggtatt gccaatatgg cagcggctgt
     2881 ttatttgatt caagaaggca aatggaaagg ggatcagatc accttttatt cattggatga
     2941 tcacggttca aatgatggtg caccgaccga cactgtagtg gatgaatatt ggaataaaaa
     3001 ccatccatta gaaaaccaaa aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg
     3061 tacctatgta gatttgatgg atctgttaag tcgaatacct tctgttactg agccagggat
     3121 gactgctgaa gaagatacga gagattttga tgcaaaacac cgaacctttg acaaagcacg
     3181 tttgatggaa ggtggcaaag gaatcattga tggcggaaaa ctgggattca ataataaaga
     3241 ccgtattctt ctgactaaac tgattgctat gcctgattca gaagaagaaa aactagacaa
     3301 tatcactatc gaagaatatt tcaaagatga tccacacttt tttgaaacaa acttctggtt
     3361 tatgtgggaa acaacatttg ctttcagaac acgtagttct gctcaagaat tgcgtcgcta
     3421 tatgcatcaa atgatttatg aattcactca aatcgaacat ctagtgggcg tcaatcgaac
     3481 acgttacaat caatacgaaa gtattatgct tcctttgatc gagtacttga aagaacaagg
     3541 atgcaaaatc attttgaacc gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca
     3601 agatgaaatc actgtgactg gtttgaaaat taccaataca ctgactcaag aagaagaata
     3661 cgttgtggtc gatgaagata cagcagttat tttcactaat gggtccatta cagattcagc
     3721 tactttagga gattacgaaa caccagctgt agaaaatatg gattatggtg cagcttctag
     3781 tctctggaaa aaagccagtg aacgtttcta caacttggga aatcctgaca aattctttgc
     3841 tgatcgagat gcaagcgaat gggtcagctt taccctgacg acgaaaaacc atttgttgtt
     3901 gaatgaaatc acccgtatca cgactcaaga accaggaaat gccttgaatt catttatttc
     3961 aaccacaccg atcacgccgt taggccaaaa agatgtcaat atgtctattg tcgtgcatca
     4021 tcaaccgcat tttacgtctc agaaaccaaa tgaaacagtt atttggggtt atttccttta
     4081 tccaagacga cgtggcgaat ttgtggataa acagtatatt aaaatgaatg gaaaagaaat
     4141 gctagaagaa ctgattggac aactttcaaa agtagatcct ggaccagtga atattcgtga
     4201 aaaagaaaca gaaattttcg acagcgtaat aaataatatc cctgtctata tgccttatgc
     4261 ttccgcgttg ttcaataatc gtgccaaaag cgatcgacca aaagtcattc ctaaacattc
     4321 gaccaatctg gcctttacag gagaatttgt cgagcagcct tatcagatga tctttactga
     4381 acaaagtgct gtacgttctg gagaaatcgc tgctttccat tttgcgggta ttcctatgtc
     4441 ccgtcttgtc aaaacaccgc gttacgacaa agatattcct actctagctc gtgccgcaaa
     4501 aaaaatgttt gaataatcaa gaggttgtga aaaagctgcc ctgcatcaag taataagaac
     4561 aaccaggcta aaacagcttc ttatgttttt gatttggttg gacttattac cgcagatgca
     4621 agcttttgaa caccgtttac caagaggtta tgaaaaagct gccctgcatc aagtaataag
     4681 agcagccaag ctaaaacagc ttcttatgtt tttgacttgg ttagacttat taccgcagat
     4741 gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa ggcgtttagt ttcgagtatg
     4801 aagaaaaatt ttgtgaaaat ggttcctcac gtttttgcat aaatttgact taataccgag
     4861 aaactgatcc cttctgtcat aacctctttt tatttatgtc tgtttctttt tgaaaacaac
     4921 tattttttca acgtttactc tcttgccaat ccaacatcat ctaacggata caactatttc
     4981 aatgattacc attacactaa aaaatcggaa acacacctct ccgaacacaa atacataaga
     5041 ctatagaatg tttcatttaa gaaaaaaagc gtttcattat caaaaaggta agatctcctt
     5101 taaaatgaaa ttatctgtag tactaataat gcagacaatt ttatgaatct gaaaggagca
     5161 tctccaatga aagcagtcac ttggcaagga aaacagaaaa tggaaatcag aaacgtagat
     5221 gatccaaaaa tattagaacc aacagatgca atcatccgta tcacagcaac agccatttgc
     5281 ggctccgatc tgcatcttta ccatcatggt gaagcagttt tggaaaaagg gtatgttgta
     5341 ggacacgaac caatgggaat cgttgaagaa gtgggatctc aagttcaaac attgaaaaaa
     5401 gggcaacgtg tggttatccc ttttaatatt agttgtggtc attgtcattt ctgcctgaat
     5461 cacatggaaa gccaatgtga tgaatcaaat ccagatcagt tatatggcgg gcttttcgga
     5521 tttggcaagt tgaacggtaa tcattggggt ggtcaagcgg aatatctaag agttccttat
     5581 gcagactcta cttcatttgt tgtaccagat aatgatcttc ctgatgaaaa agttttattt
     5641 ctatcagata ttttgcccac agcttattgg agcgttgaaa acgcaggagt aaaaaaaggc
     5701 gatacagttg tgattctagg ttctggtcca gtcggtctat tcgctcaaaa attcgctgtt
     5761 atggctggag cagagcgcgt aatcgcagta gatccagtac agcaccgtct agataaagcg
     5821 gctaattaca atggggttga aacttatttc ttagaagaca cggcacaagc tggaacagaa
     5881 ttgtatgaac tggctaaagg tggcgcagat gtcattatcg attgtgtagg aatggacgga
     5941 ttagagcctg tcaaagaaaa agcgaaaaat ttagtaagct tacaaagcgg cacaatctct
     6001 ccattacaga tggcatcgca agctgtcaaa aaatttggaa cagtccaagt aactggtgtt
     6061 tatatgactc cagcttcttc ttacccgctg caagaatttt ttatgcggga tatcgctgtc
     6121 aagcatggac aggcaccaat tatccatcta atgccaaaaa tttacgcaat gattgcagaa
     6181 ggactattcg atcctacaca aatcattact cattcgctgc cactatcaga agcagcacaa
     6241 gcatatgaga tctttgacaa aaaagaagat aaaaatataa aagttgtttt gaaaccttga
     6301 attcagtaaa aaatacaggt gattttcttt atgcccaaaa tcaaacgaaa tacccatgag
     6361 atcgaaatag gaaaagaaga agatatcgaa atacgaggac gtcgcttccg cttctatttc
     6421 caaaatcgtt acacattgat ctcactggct gtcgatttat taaccgggat tttctatatt
     6481 tttggaagcc ttgcttcctt aactaaaatc cccgatcgat atggcaccta tttttatttg
     6541 gctggtgcta ttttcttgac gattcgtcct attctcagaa tcgtaaggaa catttttatt
     6601 tacgacgaaa aaaaagcaat gaagagaaca agcaaaaaaa aagaggcatg agaatttttc
     6661 aaatctcatg cctctttttt caccctatcg tatcactttg atacagacga gcggtcgtcc
     6721 cactttcttc cttgaccatt gtcaaaaatg tccactcgta tcgttcataa aacgtggtat
     6781 gatctgtaat taagtaaagt ttttcgtacc caagttgatg catttctttt ctgatttccg
     6841 ctaatatccg cttggctatc ccctgcttac gcttttccgg ttcaacaaac agggcacaaa
     6901 gatttggtgt cagatcaggg cggttatgga agtcgttttc aatgacgccc gctcctccga
     6961 cgatggtttc tttttcatct aagaccacat accattgagg aatactttcc ttattagcga
     7021 ttccagtacg aatactttcc tcatacacaa cagcaggaac tccccatttt gatgaaaacc
     7081 aattcgcgct ttgttttact aattctgggt gttctcttaa tttgatccag ttatatgtaa
     7141 ccaatgttta aacctccgac taaaataaaa cgtttacaaa aattatacaa gtctttatga
     7201 aaattagcaa tgacaacaat agtgatgtaa aaaaaaaaac ggaaaacaat cacttgttct
     7261 ccgttttttg attaatcaag catctaaagc ttcttttctt ctttcttgga tcgtagctac
     7321 cttgacgaac ggtagataga tcaaaataga aagaaccaaa ttgacagcgg ctagtacacc
     7381 acctgcaata ctttttgttg ccaatacacc gccaataatt ggtggagtta cccatggagg
     7441 cataacggta gctgctggca ctaaacctgt agaagtcgca aagtaagcaa cactaactaa
     7501 gaccattggt gtcaaaataa atggaatgaa catgatcggg ttcaacacga taggtaaccc
     7561 aaacatcgtt ggttcattaa tattgaagac acctggcgca atcgacaagt ttgttacaac
     7621 catatatggt ttgtttttac gtcctactaa ataaatagct aacagtaaac ctaaagttgc
     7681 acctgttcct cctaagttga caaagctgtc aaagaaaggt ttgttcacga taaacggtgc
     7741 gctatgtcca gctgcgattg cattaacgtt tgcttcaatg gctggcgcat tgattgtttg
     7801 catcaaagga tcgaccatat tcgctccgtg taaaccaaag aaccataaga atggtgtgat
     7861 aaatgcaagc agtaaggcag atggataaga gtttgcaagt cccataaatg gttcttggac
     7921 cactgtatag aaagaaccta cgatatcatc aacaccgaat gcagcaaaaa ttgcagcaac
     7981 taaagcaaac gcagagatag tgatcattgc tggtagtaat gaagcaaatg cttttgcaac
     8041 tgccggtggc acaccatcag gcattttgat gatcaatttt tcatttccgc ttaaacgtac
     8101 aaagatttct gttgaaataa tcccaacaat aatagcaata aacaaaccgt ttgagctcat
     8161 acctaatgct ccgcctaaag cgaagaaaga gcctaaagaa atcacacctg cggaaacacc
     8221 gtcttttcgg tagccgttag ctaaatgata agcaagcaag aaagcaatca agacagaaag
     8281 aatggcaaat gtaccgttcc aagcagcagc cccgaatcct ttccatgatt caccaccaaa
     8341 aattgagttc atcaattctt ggaacccggg aatcggtaag ttattgatca ttactgctaa
     8401 tgctcctaag atcattaacg gcatactcac cataaatgag tcccgaatcg caactaaatg
     8461 ccgttgtgca ccaatttttg atgctacagg tataaaatgt ttctccataa atgcaacaaa
     8521 tttatccata aatcttccct cttttcttta ctgtagatta cgcgttcaat cgacgataca
     8581 gatcaatgat ctcttttgct aaatctgtaa aagctataga tgtcatcaga tgatcttgac
     8641 tgtggaccgt caaaagcgtg acttcaacgt gatttccttg tgcttcttgt gtcagcatct
     8701 ctgtttgaga gtgatgtgct tggactaaag attcctctgc atctttgatt ttttcatctg
     8761 ctaaagtgaa atcacctttt ttcgcagcag caatcgcttc cattgcatcg ctttttgcat
     8821 ttccgccaaa catgatcaga cccatgatgg cttctagatt tttttgatct tccatttatt
     8881 gtatcctccg ataattttta ttatttgttt attagttttt ctgcttggtc gagtactttt
     8941 tcaccattca tcatgccata gtcagccata ttgattacat ctaaaggaat gcctttaggt
     9001 gctagctttt cagcaaattg attttgcata aagcgtactt gcggtcctaa aagtaaaacg
     9061 tctacatttt tagattctag ttgattatcc gcatctgaag cagatacagc aaagatgtct
     9121 gcttccattc cttttgcttc tgctgccttt tgcatttttg tgactaggag acttgtactc
     9181 attccagctg aacatacaag catgattgtt ttcttttcca ttttaaacac gttcctttct
     9241 atacttacac ttgtatataa gcaagtagcg tgccaacttt aaaagcgctt taaaaagttg
     9301 atatcatcac atttaaggtc tataccaagt tgcatacaca ctaaaaaaga aagcgcacac
     9361 taaacacaca aattaatttt tattaccttt tttcttttaa cgtgcacaaa aaaagttgtg
     9421 acacaaaact tgtcacaact tttttcaaat aaacggtatt caaaagcttg catctgcggt
     9481 aataagtcca accaagttaa aaacataaga tgttgttttt actttgctat tcttattact
     9541 tgatgcagga cagctttttc acaacctctc aataaacggt gttcaatcag ctgaccctgg
     9601 gtattagctc aaaacgctgg attcacaacc tcttttattt caaataacca gtaaatacaa
     9661 tgaacatcta caaaaaactt ctatttattt atagatttcg tcaggcaaat ccaattctct
     9721 cttatcaatc aaagtctgat accacgaagc agattttttc aatgttctgc ttcgttcatc
     9781 gttcaattcg attcgaacta atccatatcg attcttaaat gcattcattg gtgacacaca
     9841 gtcagtgaat gcccataaca tatagccttc gcaatttgcg ccttcttctg ttgctttgat
     9901 caagtaatgc aaatgctgtc cgataaattc gatacggtaa tcatcttgca cgattccttt
     9961 ctcatccata tactgttctt catattctct tcccataccg ttttctgtta tcagccaagg
    10021 gatattatga taattttctt ttagatacat ggctatatca tacatgattt tcggatagat
    10081 ttcccatccc ctggaattat tcattttctt cccaggtaaa tcaaagactt cataatatcg
    10141 ttcaggatgg aaaggtgtct cttcattcca ttgataacga ggggcgcgaa cgcgtttagg
    10201 ataatattga ttgattccaa ggaaatctac tgtatgatgc tgaatcgttt ctaaatcctc
    10261 tgttgcagga tcgaaataaa tcgattgttt ttgacacaac tccaggaact cttctgtgta
    10321 gtatcctttt accatcggat caaaaaatac tcgattataa aacaaatcat acatataagc
    10381 agctttttgg tcttctggag aattggatcg agcatagacc atttcaggat ttaaaataca
    10441 gccaatcctt ccaccataat ttccgctgcg gtaaatctct actgctttag ccgttgctaa
    10501 aactttatga taattccaca tcatccactt ttttgtgttt tgttcatgcg gccagcgaac
    10561 agcatccaaa tagcaacgcg tttggggaac gattggttcg ttgaatgtaa accagttttt
    10621 taccctatca ccgtaacgat caaaggcaat ttgagcatac ttagcataaa aatcaactac
    10681 ttttcttgaa gaccagccat catatttttc caaaagaaaa gctgggactt cataatgttc
    10741 caaacaaatc atcggttcta cgtctgcttc gatcaaagag tcgatcattt cacttatatg
    10801 ctgagcatat tcttcatcta ctatacagtt ctcataatca gtaaaaaaac gagcccaatt
    10861 gatagaagtc cgataatgtg tcagatggat ggatttcatc agatccacat cctgctggta
    10921 ttgttccata aaattagttg ctacggctgg gccataccca ttatgccaaa caaaatgttc
    10981 atttttatac cagctatcta taaacgaatc ctgtccttct ttttttcctt tccaaccttc
    11041 cgtttgccaa gcagaagatg cagcaccaag aataaaattt tttggaatag taattttcat
    11101 gatttcctcc tgttttttag atcccatcat tttccacacg atttgctact tttacaaatg
    11161 gcagatagat caaaatagaa acaataatac aaacaagttg cgtaataact gcaccgatat
    11221 ttcctgctgt tgccagataa gcattgacga tcggcggcat tggccaaggg accattacaa
    11281 ctgctttcgc cgcaaatcct aaagcggttg caaaataacc aatcgttcca gtgataagag
    11341 gcgttaagat aaacggaata gctaaaattg gattcagcat gatcggcata ccaaaaataa
    11401 ctggttcatt gatgttgaag atcccaggag caagggataa cttggtgatt tgtttcatat
    11461 cttcccgttt tccaactaac aatactgcaa tcagtaaacc gatcgtgact ccagacccgc
    11521 ccatactcat atacatatcc caaaatggca tagtaataat gttcggtact tcttttccag
    11581 cttcaaaagc ttccgtattt acagcaattg ccgctaaaag caacggttcc cgtatcggct
    11641 tcaccatttg atttccatgg ataccaatca cccaaaagac ttgcgcaata aacatcaata
    11701 gcaaaatacc cggtaaccct tgtacgacac cttctaatgg gcgttgtacg acattgtaaa
    11761 tgatatcata aaggtacatt cctgtaagtt ctttgatgat aaatcccgca gccgcgatac
    11821 ccgatacagt cagaatcgtc gggaataaag cagaaaaact agcagaaaca ttcgttggca
    11881 cggtatcagg cattctcagc tgtagtttct tctgtttcct caaccagcaa taaagttcaa
    11941 tagaaaggat ggagaccaac attcccaaaa ataacccttt cgtatctgta tattgtttag
    12001 ccaatacgtt ttctactata acagtctgat tttcattcac taattcataa acagttggat
    12061 taacagataa ataagacatc actgcgatga taccagggaa tacaccatcc actttattca
    12121 gcttggctaa ttcgatccct atcagaaaaa cagcatagat ggtcaagaaa cttagtgtga
    12181 catagctgat ggcagatgcg ataggcttta attcttctaa aaaacgcaag aaacttatct
    12241 gtgctaagcc attttccgag tcaaagacca ttgctgaaaa caatgtgcca aaagcacccg
    12301 taataattac cggaatcaat gcagcaaatg cgttttttat caccatgata taccttaaat
    12361 tattgattct cgttgccacg ttccctaaaa cattggctaa tttatccata aaactcattc
    12421 cagatcccct cattcctgct tcttctcctt gacgtcatct ctttttgtct attagtcagc
    12481 gcttacattt cgatgaacag aaactattta ctcctctttt ttaccaaata taaaacaaaa
    12541 attcatctga tgtatttatc ataaaataca tcagatgaac tgtcaattag tttctttttc
    12601 tcctattatt ttctgtaatt ctctccgatt tattgccata tggacagtca ttgcatcaaa
    12661 cgcagcaagt gcattccgcc tttcgatggc ctgaacgatc tctgtatgaa tatcgatcgt
    12721 ctcgattttc agatgctgga tatcataatt tttattaaac aaagcaattg attcattaat
    12781 gattgggata atatgcagca aggctacgtt tccgctagct tctgcaatca tcgtatgaaa
    12841 atcgatgtct aattgaacat gttcttgtgt gccttctcga aaagaagttt caatcttttg
    12901 cgttaatttt ttcatttgga cgatttgatt cttcgttgca tattccgctg ccaaacctgc
    12961 catcttaggc tccaataaat atctcagttc aaataagtct cgtatcatcc gatcctgatc
    13021 ttcaatcaaa gaaaaaccga gtgggtcatt ggcaatccct cgcttatcac tgacaaatgt
    13081 ccccgctcct tgccgaactt caaggatatt cctggaagct agagcccgca cagcttctcg
    13141 caccgtgctt cgccccacct ctaaataatc ggacaattca tattcattcg gcagtttatc
    13201 tcctacttgc aactgcattt cctttaaata tctaaggatc ttgtcagtag ttacttctac
    13261 tagggtttta ttttcttcca aaccacatac ctccttttca tctgatgtat tttttcgtaa
    13321 ttattataac acgtttgaat catctgatga ttttttgtat acaaaattga ttaatccgtt
    13381 catagacata caaacgaaga agatatttac cataaaaaag actaggataa aattcactcc
    13441 tagccttcga tcatttttat tttgaacgac aaaaaatatg atgttcaata acagacgatc
    13501 ttcattcgat tcttgatgtc atcttttgtt ttttttaata tatggataaa aagtttgatt
    13561 acatcgtttc tattctagaa tttggtcgat tctcaattct tcttctggtg agaaatctaa
    13621 attatccatc attcgaacac tttcttgtaa ctgacttaac cgacttgctc cgaccagtac
    13681 acttgtaaca gatttttgtc gcaaattcca tgcaagggcc atttgcgcta atgattgtcc
    13741 tcgtgatacg gcgattgttt gcagtgcttt gattttttcc agagttgatc caacttgttc
    13801 ttctgaaagg aatggtattt ccttacgatg tgctctagaa tcttcaggaa taccatgtag
    13861 ataacgattt gtcagcaacc cttgagcaag cggactgaac gtgattgccc ctagcccttc
    13921 ttcttctaat actggagata gatcgtcttc tagtagtgca cgagaaaaca tattgtagcg
    13981 catctgatga ataatgaacg gtgcttcctt tctttttaaa atttcagtca ttttttttgt
    14041 atcttctcca ttatagttgg atataccaat atataatgct tttccttgtc ttacaagttg
    14101 catcaaagct tctgctgttt cttccaaagg tgtatcaggg tctgggcgat gatgatagaa
    14161 gatgtcaaca taatctagcc ccattctttg gagactttgg tcacaacttg aaatgatgga
    14221 tttacgagag ccccattccc catatggccc ttcccacata tgataacctg ctttgctaga
    14281 aataatcagt tcatcacggt aggcttgaaa atcttctcta aaaatacgcc caaagttttt
    14341 ctctgcagct ccagcagggc ctccataatt attcgcaagg tcgaaatggg taattcccat
    14401 gtcgaatgcg cctcttaaaa tctctctttg attatggata ggatcataat caccaaaatt
    14461 ttgccataat ccgagagata aaatcggtaa tttcaaacct gaatgaccgg atttacgata
    14521 ttccattttt tcataacgtg cactatctgc aatatacaat ttttctcctc ctcatcaaat
    14581 ctttttaaaa cgctttcttt tttctatttt tagtttacta cattttaaag aaaaggagtt
    14641 aatcaaaatg attttttcgt atattttttt gccatttgtt ttttactcgt tgatagatca
    14701 gaaattgatt acgtgcttgc tggatttttt tataacgttt cggttgtttt ttgtaaaatt
    14761 gctgatgata gttttctgct ggccaaaacg taccggctgg ttcgattttt gtgacgattg
    14821 gttggtggaa agttcctgaa ctgtctagtt tctgttttga ttgttccgct aattcttttt
    14881 gtcgttcgac ttgataaaaa atcactggac gatattgctt ccctctatct tgaaattgtc
    14941 caaatgtatc tgtgggatca gtaatttgcc aatagattgt cagcaactcg ctataactta
    15001 tttctcttgt gtcataaatg atctccacag cttccacatg acctgtgtaa ccaccactta
    15061 cttgatcata atttggtttt tcaacatgtc cgcccgtgta tcctgacaaa acagatagga
    15121 tccctttttt cgattcaaac ggctcgacca tacaccagaa acatccccct gcaaatactg
    15181 cacgttcttg atagtcagca tcttgcatgg catgcttgga aaagtcatat ttttgctcct
    15241 tatcaaatcc ttccgtaatc aataaataaa agtccattac atcaggcgtc agattcattc
    15301 ggatagctaa tggacgtagt gttgcttcta gttttgataa ctgttcttta acggatatat
    15361 tttctcttat gtcattcttt gtctgaatca atacttttcg ttcccaatct cgtgtattcg
    15421 gattcaaaat cagattataa agtgtttgaa tgaatatttg ttcctcatcc atagattctc
    15481 ctcctttccg aagaagacaa gctagtgact ttagctgtaa tttatcataa agtgaggaaa
    15541 taagcgatta cttcatcccc ctacagattt ttcgtataaa aaagagagaa tccttatcga
    15601 cagattctcc tttttaatca atttatttta taaatgaaaa gttaatttac cttttatgga
    15661 aaactatttt aaaaaataaa cataaatatg cagttaatgt aacaagataa gaaataatag
    15721 tcgttactgt ataacttgtg cttactttaa tatcagcaac tcctttgact ccgcttattt
    15781 gtaccaatcc attagcattc acctgctcct ttacaggttg atgatttatt tctacattcg
    15841 ttcctaaata agcatataca ggaagtgtga ttacttggct cttctcatta gttactctat
    15901 atatgataga actaccagtt gttttataag gaaggtttac ccattgactg ccaatctttg
    15961 cttcgtgttt tattatatgc tccttattct ccaatgaagc gataggagaa taatcatata
    16021 aaaaataatc cattccttta gtggtatacg cctctactgt ttctttgttt acaacaatat
    16081 gtcccggctg agaaattatt ttttgattga aatttttttt actagaaaca gttaaagtga
    16141 ttaataacaa aacagtcata aataaataaa atagttctct tgtcttattg tatcttctat
    16201 ttttgaaaaa tatcatcgtt aatatcagag aagagaatat tatttgcaag cttaagatcc
    16261 gccaaggaaa ttgtaacaat ttggctggcg tattttgcag aagataccat ggcaccaaac
    16321 tagttgtagc tagccaagtc aaacacgtac cataataaat ctttttttct atgtttgtta
    16381 attttttaaa taaaatcagg ggaatacata aagataacag accaactagt cccattgagt
    16441 aagtagttaa gtcgttgttc atagcttcag tcagactatc gaacacatta gatgcatgag
    16501 catataaatt tgtcacgcta ggtttgttga ttttttggta gagcatttgt tcaaacatcg
    16561 gataccaaaa agcactcgtc agaaccaatg tcattcctgc cgcactaaat aattttttaa
    16621 atatagcaat aaaatctgca ttcttgaaac ctttagaaac caaacgaata aaaataaaaa
    16681 tgataattaa tgctgtactc attaatgttg atagtacatg gctgtacact agcaaactca
    16741 tactcaatgc tagctttaca gacggatact tttcttcttt tccagagatg atttgataag
    16801 cataaaataa tacagggata acaagtgtta ttgcgatagc ttcggcaatt gcggaacgat
    16861 agtagatgtc tactgtccga taagcagaga aaatatagag acaactaaat aaaaatgccg
    16921 cttttttttg ttttaaaaac cgctcgccat aatgataagc tacgagtgtt gtacacaagt
    16981 tcaataacca aacgtaaata atatagctta caatcaaatt ttttgtcatc caatataaca
    17041 ttacagctgg aaaaaaagtc agaaagggat aaaaataatt tacaccatat ccagtataat
    17101 tgaatgtctc ataattaatc ggactggtaa aaatagtcga taaccccttg attcgagata
    17161 agtgaaaaga aagatctcca gtggtataaa tactctgtgt atttttgaaa aagatgaaag
    17221 aatatatact actaactata attaatccac tcaatattac tactttttta tttattttca
    17281 ttcttcttgt ttatctcctt tttagtcaca tatcgagaat ctttcaactt attaggtgca
    17341 aatcgtttaa aacttgaata caccactaaa caataagtca gaatactgat ccataaagcc
    17401 atgagagcag ttggagtata ttttgttgta atttctatct tattttctcc agcatttaca
    17461 tccacattca ctaagccatg ttttttataa gagtcattta cgactttacc gtttactttt
    17521 acctgtgtac ctaaataagc atatactggt actgtcactt tttgcttttt gttgcttgcc
    17581 actgtgtaag taatgttatt atctgcagct ttatacttcc ctttttccca cgattctcct
    17641 acttttactt catgtttttc taagtgaggc ctttctttta tcgcttctgt tggggcgtaa
    17701 tcgtataatc ctcccatatc tgatgttgta taacgtggga gtgtttcttc attgataaca
    17761 atatgaccgt gttttgattg gataactttt gcgtaatttt cttttgctgc tatagacgtg
    17821 acgagtagaa acaccacagt agcgccaatg attaaccatg aatatttttt attcatagtc
    17881 tttttcataa aaacaagcgt tatgatcatt gaagaaaaaa agatttggat acctaagata
    17941 cgccaaggaa actggagtaa gcttatcggt gttttttgaa acaaaaacca tggaaataaa
    18001 ttagttacgc atacccatgt agaaactacg ccaacataga tgattttttc ttttttctcc
    18061 atttctttca gtatgaataa aggaaaaatc aacgcaacca atcctaataa ccccatagtg
    18121 tatgttgtaa tatcgttatt taacgcttga atgatactat ctgaaataga aagtgcttct
    18181 agctgcaaat ctgttttgaa gggactgtgt atttcttgat aattcatctg ctgcaacata
    18241 ggataccaaa aatagctagt taatgcacaa gtcaaaaaaa cagcctttac taaattcacg
    18301 accacaaatt ttatatcgtt ttttatttgc ttgcttaaaa acaaacgaac tataatcata
    18361 caagcaatca atgcactaca caatagtgta gttaagacat gagtatacac aagtagactt
    18421 atactgacag ataaagcgat gacttttttt tctctattga atatcaatcc ataagcataa
    18481 tataaaacag ctgggataat cgtaagagcg attgcttctg caattgcgga tcgataatag
    18541 atatcaactg ttcgataagt tgcaaaagta tataaacaac taaataaaaa agcagcatct
    18601 atctttttaa aaaacttttc tccataataa aaactaatca g
//
LOCUS       DASJTC010000058        18035 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.058, whole genome shotgun sequence.
ACCESSION   DASJTC010000058 DASJTC010000000
VERSION     DASJTC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.058"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(558..920)
                     /locus_tag="U4B19_001807"
     CDS             complement(558..920)
                     /locus_tag="U4B19_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEN2077543.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            1143..1895
                     /locus_tag="U4B19_001808"
     CDS             1143..1895
                     /locus_tag="U4B19_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2077544.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(2035..2958)
                     /locus_tag="U4B19_001809"
     CDS             complement(2035..2958)
                     /locus_tag="U4B19_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2077545.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2973..3245)
                     /locus_tag="U4B19_001810"
     CDS             complement(2973..3245)
                     /locus_tag="U4B19_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077546.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(3589..4776)
                     /locus_tag="U4B19_001811"
     CDS             complement(3589..4776)
                     /locus_tag="U4B19_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEN2077547.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(5189..6814)
                     /gene="groL"
                     /locus_tag="U4B19_001812"
     CDS             complement(5189..6814)
                     /gene="groL"
                     /locus_tag="U4B19_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEN2077548.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(6865..7149)
                     /gene="groES"
                     /locus_tag="U4B19_001813"
     CDS             complement(6865..7149)
                     /gene="groES"
                     /locus_tag="U4B19_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEN2077549.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            7374..8036
                     /locus_tag="U4B19_001814"
     CDS             7374..8036
                     /locus_tag="U4B19_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2077550.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(8112..9404)
                     /locus_tag="U4B19_001815"
     CDS             complement(8112..9404)
                     /locus_tag="U4B19_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2077551.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            9574..10203
                     /locus_tag="U4B19_001816"
     CDS             9574..10203
                     /locus_tag="U4B19_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEN2077552.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            complement(10306..11115)
                     /locus_tag="U4B19_001817"
     CDS             complement(10306..11115)
                     /locus_tag="U4B19_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2077553.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(11170..12039)
                     /locus_tag="U4B19_001818"
     CDS             complement(11170..12039)
                     /locus_tag="U4B19_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEN2077554.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            complement(12040..13356)
                     /locus_tag="U4B19_001819"
     CDS             complement(12040..13356)
                     /locus_tag="U4B19_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077555.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            complement(13353..15188)
                     /gene="walK"
                     /locus_tag="U4B19_001820"
     CDS             complement(13353..15188)
                     /gene="walK"
                     /locus_tag="U4B19_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEN2077556.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            complement(15193..15897)
                     /locus_tag="U4B19_001821"
     CDS             complement(15193..15897)
                     /locus_tag="U4B19_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2077557.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            complement(16087..16947)
                     /locus_tag="U4B19_001822"
     CDS             complement(16087..16947)
                     /locus_tag="U4B19_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2077558.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            complement(16934..17503)
                     /locus_tag="U4B19_001823"
     CDS             complement(16934..17503)
                     /locus_tag="U4B19_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEN2077559.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            17884..17956
                     /locus_tag="U4B19_001824"
     tRNA            17884..17956
                     /locus_tag="U4B19_001824"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17917..17919,aa:Lys,seq:ctt)
BASE COUNT         5228 a         3791 c         3073 g         5943 t
ORIGIN      
        1 tttttctgaa aaagcacatg ttttacggat atttcttgct aaaaatgaac tccatctgct
       61 aaaactatcc tttcttattg gtatttaacc taaacgagaa agaatactta ttttattcaa
      121 tgtatccgag acacgaagaa attagcgttt tctatttttc ttacttgata aaataagtag
      181 attttcctac tacaatcgta gccgttaaga taccgatgat tgaacaaata taaccaaatg
      241 tctctacaac ataatcaatg gcaaaagttt ttgaagcaat aatacgtctt tccttttttg
      301 cttcttatcc ccaaagaaac ggatataact tattatgtat tgaatttttt tggaaaggcg
      361 aattatgaca agatatacct tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt
      421 aaaaagatac aaagtaagaa agcagctgtc aatagaatgg aaacaaaaat ggcgagacat
      481 caatcagtct cgccttgttt tttattgtat acattagata tggagcaggt taccataaag
      541 atgattatta gtcgttatta aatagaatcc ttcgtatttt tttgttctat cactacaata
      601 tctttatttg gacgtttgaa ttgtattccg gcacgttcta gctcctgttt cactcctaca
      661 tggactttga catcttctcg tgtcattttt aaaacgtgat gaatcgcata gtcgatttct
      721 tgccttgtga tttttttctt cgtattgatc atcacgactt gctttttatc atatgtgtct
      781 accaatagca gttgtgttcg atctaacgaa aataatttgt aataattaat agccgtattc
      841 ccaaaaaaga attccactac tttaggataa aatgtcttaa tatttaaatt actattcaca
      901 ggtgtttcga ttagcttcat ctcattcctc ttttctatat agtcgattta gatccaatct
      961 taagttatgt taccatcttt cctagcatta aggaatcggt tacaattgaa cctttaaaga
     1021 caaaaggatt taccttttat cacttaatag tataacataa tttcgtgaga aaaaaataca
     1081 attttttctt gaccatctcg ttacgtaaag gattatgctt acatttgtca ggaggaaaaa
     1141 taatggagta cacaataaag aaaatgtcag agatatcagg agtcagtccg agaaccttgc
     1201 gcttttatga tgagattgga ctgctaaaac cagctaggat caattcttca ggttatcgga
     1261 tttatggcaa aaaagaagtc gatcgtctgc aacacatctt attttatcga acaatggcat
     1321 tcaagttgga tgatatccaa gaggtcttag acaatccatc ttttgaccac cagaaagctt
     1381 taatcaagca tcagcaaatg ttgctggaaa aacgggcaca aatcgatacc ctcttgacaa
     1441 ctgtccaaca gacacttgat atgtacgaag gaggaagaaa aatgagtgac aaagaaaaat
     1501 ttgaagggtt taaacaacaa aagttgaaag aaaatgaaga atcctatgga caagagattc
     1561 gacaaaaata cggaaaaaaa aaggttacag catccaatga aaaatggatg aatatggata
     1621 aagaaacgta tgaagaaatg caaactgttg aaaagcagat gttatttgat ctcactcttt
     1681 acttaaaaga accagaagat gaaagcttag cagaccgtat cttccaagct cataagaagt
     1741 ggttctctta cagctggcct gaatatcagc caaaagcgca taaaggacta ggaatgatgt
     1801 acgaagccga tgaacggttc actgcatatt atgacgagcg aagtggtcat ggtgcagcaa
     1861 aagctttaaa tgaaattatc caaaaaaagg cgtaatcttt tacatgatcc aacaaaaaag
     1921 tacacgtaaa atgacctaat aacaaaacag cagacaccca agttagattt ttatgctaac
     1981 ttttggtgtc tgctccattc tttcacaagt tttttcatcc tactttaaat atattcatcg
     2041 ttgaataaac gtataaggta ttttcacttt atcctttttt tctgtaagaa acaattgaaa
     2101 tgccatttct ccgcaagctt ccagatggtg gtcgatggtt gggaaaccaa gaagctcgct
     2161 gatcagcaaa ttatcgcgtc caatcaccac tggtgtctta tcttgcagat agctttgcaa
     2221 gatgcctgct gcaaccatat ccgaatttgt aaagacacca tcaactccaa gctctttaaa
     2281 aaagaagcct gcttgttttc ctttctctgc atctatacaa tcccaaaaaa caagtgattc
     2341 atcaaaagat ggaaaaagtt gatgacatag ctgcaaagtg atttttgagt tataacttag
     2401 cctgccactt cgccccaacg tgatgcccaa ttgctttata ccttgctgct tcaaataagt
     2461 aagaccttct gtaattgatt gttctcgatc aataaagaca cagcttgcct gttttccctt
     2521 gatttcttca caaaaaacta gaggtccata tttttgatat gccaataaat catctagtgt
     2581 attggcacga gacgtaatga tcaatccatc gaaagcttta gctgcaaatt cttcaagata
     2641 ccttcgctct ttttctttat gatagcccgt cggtaaaagt gtcactttgt aatcataatc
     2701 aaacgctgct tcaatgatcc cactgatcaa ctgatcaaaa tacggatgat tggtataagg
     2761 cagaatcact ccaatgcttt tcgtttttcc ataacttaac tgtctggcat ttgcattagg
     2821 gatataatcg acttctttca tgaccgcaag tatttctttc cttttttcct catctacata
     2881 tggatgtcca ttgattacac gtgaaatagt agaaactgaa taacctgtga gctttgcgat
     2941 atctcgaatg tttgccactg ctgcttcgcc acctatacaa aaagattttc ttgtaaatag
     3001 tctaccacag ctggaccgca ttcaaaaaac tgatacattt ttccttcatg cgaaaaactg
     3061 atccgctgcc cgccccaata tatatctttt gtcaaatttt cgatttcatt cattggtatt
     3121 tcttttacta tattcctatt caacccttga aaactgtaca acgttaatac ggatcccttg
     3181 aactcgacaa acgcataaaa ataattgttc gttgtgccat ctgttatctc tacataagta
     3241 ttcattgcaa gccctccttt tgatactttg agtatagtgt atgaaacgcg tctcatagca
     3301 aggagtttgt tttgccaaag aaaagtttta ttcctctttc attattacca aagtctctta
     3361 ttttaaattg aatacattat aatgatactg taaatatatc tttattatta gtgaatggag
     3421 tcaagtccac aatcttgtgt aaaagttcaa cctcatcaaa agcacaatgt tttgcgttgt
     3481 gaacctaact gccatgcctt tctttctgag gagagaggtc aggagcagta gcgcagcgac
     3541 tcttgatgtc tctcctcaga aagaagagac tcgcacaggt tcatggtgct aaatgttatt
     3601 cgaattaaat tctaaatatt ttcgtggaga agccagccac tcagtgtctt tttccatcaa
     3661 aagtgttcca attatccgag tgatggaatt gatatttgga aaaatccgaa tcactcgttc
     3721 tctccggcga atctcttcgt tcaaccgttc caacacattg gttgtacgaa tccttcggcg
     3781 gatattttca ggaaaagaca aaatttgaat agcatcttca aagccgtttt ctaaaatgtc
     3841 gcaagcagca cttaattttg aatcacaacc atatttttcc aagaagtgct cttttctttc
     3901 acgtgtcaat tcaagttcag aggctttaaa gatactcttc aactcgtctt ttacatcaga
     3961 agaaactttc tttggcagct tatcaaaaat atttctcaaa aagtgggctt ggcatctttg
     4021 ccagctcgca cctaaaaagt tcttgcgaac agccttaact aaccctttgt gtgcatcaga
     4081 aatgaccagt tttacaccga acaaacctcg ttgaacgaac gattgaaaaa cggtatccca
     4141 attatcttcc gattcgccgt cttgaacatc aaaaccaaga atcgtacgtc gtccatcaga
     4201 atcaatgccg ataccaatat atacaccttt tgagacaata cgatgatttt cccttacttt
     4261 catatacagg gcatcacaca tgaaaaatgg atattccgag ccttctaggc tccggtttct
     4321 ccactcaaaa acaaatggat ccaactgttt catcagattt gaaacgaatg atttagaaac
     4381 agtcgcacca tccgttagca gttcaactgt tttagttact ttgcgagtgg agacaccaga
     4441 aatgaccatt tcaatcatgg cagcgatcaa agctttttcg cttcgctgat atctttcaaa
     4501 gatagtagga ctaaacttac cgtctctggt tcgtggaaca tgaagttcta acgtaccaat
     4561 tcgagttgta aacgaacgat cgtagtaacc atttctttgt gattgccgtt cttctgaacg
     4621 ttcataggca tttgcctgaa tatattgatt tcgttgttct tccatcagct gattaaacat
     4681 gatagtcata agtgtttttg cggtatcatt cgcaccgctt tcgtttataa ttgcttgaat
     4741 ttcttcgctt tcaaaagtaa aatgtacttg agtcattgat atcagtccta ttcgagtatg
     4801 ttttctgcaa aaaacattgt actcctaact gagatcaatg gcttttttga tgcttcactt
     4861 ttacacaatt atatggacgt aatcgtgaat ggatatttac ttttaataga taaaatatta
     4921 taactattaa aaataactat tttcattata attacacaca aaaaagagcg tgggacaaaa
     4981 gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cagcggaagc agaagcaact
     5041 ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aatttccgtg aatttcttct
     5101 tatttcttgg agttaaacgc ttctgttcca gcctcttttt atctgtcttt cactaatgat
     5161 cagttgtaga taagcaagtt gctttttctt acatcatacc gcccatcatt gatggatcca
     5221 ttgcaggagc tgctggcgct gctggttctg gtttatctgc aacgactgct tctgtcgtta
     5281 ataacaaggc agaaacggaa gcggcatttt gcaaggcaga acgtgttact ttagttgggt
     5341 ctacgatacc tgcttcaacc atgtttaccc attctccagt agcagcattg aaaccagtgc
     5401 ctagttccac gttcttcaat ttatcaacaa tcactgatcc ttcataacca gcgttttcag
     5461 cgatttgacg aattggttct tctaaagcac gaacaacgat cttgatacct gttgccacgt
     5521 ctccttctgc ttctacagca gaaactttgc tgataacgtt cactagagct gtaccaccac
     5581 ctgagaccat tccttcttct actgcggcac gtgtagcatt caatgcatct tcaatgcgta
     5641 attttaattc tttcaattct gtttcagtag ctgcaccaac tttaacaaca gctacaccac
     5701 ctgctaattt agctaagcgt tcttgtaatt tttcgcggtc aaaatcagaa gttgtttcag
     5761 caatttggtt tttgatcaat tgcacacgag cttcgattgc ttctttttct ccagaacctt
     5821 ctacgatcgt tgtattgtct ttatctacaa caactttaga agcatttcct aagttttcga
     5881 ttgttacatc tttcaattct aaacctagat cgtcagtaat tactgtacca cctgtcaaaa
     5941 tagcgatatc ttcaagcatt gctttacgac gatcgccgaa accaggagct tttacagcta
     6001 ctacgttgaa tgttccacgg attttgttca atacaagagt tggcaatgct tcaccatcta
     6061 catcgtcagc aataatcaac aatggacgtg attgttgcaa gatttgttct aacaatggca
     6121 gaatatcttg gatgttagag atttttttgt ctgtgatcaa gatatatggg ttttctagaa
     6181 cagcttccat tttgtcgtta tctgttacca tatattgtga taagtaacca cgatcaaact
     6241 gcataccttc tacaacatct agttctgttt cgatcccttt tgattcttcg attgtgatga
     6301 caccgtcgtt tccaactttt tccattgcat cagcaatcaa atgtccaact ttatcagaac
     6361 ccgaagaaac agctgctact tgtgcaatgg cttcttttga atcaacaaca gttgagatat
     6421 tatgcaattc ttcgactgct gcttttgttg ccaattcgat cccgcgacga atgcctagag
     6481 gattagcacc tgcagttacg ttttttaaac cttcgcgaac gattgcttgt gtcagaacag
     6541 ttgctgtcgt tgtaccgtct cctgcaatat catttgtttt tgatgctact tctgatacaa
     6601 gttttgcacc catgttttca aaatgatctt ctaattcgat ttcttttgcg atcgtcacac
     6661 catcatttgt gatcaatgga gagccgtaag atttttctag tacgacattt cggcctttag
     6721 gtcctaatgt tactttcact gtatctgcta atttatctac cccgcgtaac atagctgcgc
     6781 gtgcgtcttc tgcaaatttc aattcttttg ccatgattga ttcacctcat tgattatttt
     6841 tgattatttt tttatcttcc aaatttactc aacaatcgcc atgatatctt ttcctgcgac
     6901 gatcagatat tcttttcctt cgtattttac ttctgtaccg gcgtattttt cgaacataac
     6961 ttggtcgcct gcttttacag cggcaggaac tttttcaccg ttttctaaca aacggccttc
     7021 accgactgca acaactgtcc cagtttgggg tttctcttta gcagcagaag ctaaaacgat
     7081 tcctccgaca gttttttctt cttcttttgc gacttcgata atgacgcgat cacctaatgg
     7141 ttttaacacg ataaatccct ccgataactc tttttttaat ctgttagcac tcttaacctt
     7201 cgagtgctaa cctacatttt taataatact cattctccct cttttttgca agcttttaaa
     7261 tcaaagaaaa gaaattttat tctgacagct gcttattgaa attatgatat aattgaaatc
     7321 aatcgatata agcttgcctt gcatgcttgt tatcccttaa aaggaggaat tacatgtctt
     7381 taaaaaaata tagcttcctc agcatttttt gctatggtct aatttttatc tcacccttga
     7441 tcctagcttc gataggactt gtaaaatcta cgtctgaatt gatcacagca actgcagtca
     7501 tgtatatact aggcgccgtt gtcctagcta ttttttattt taagcaacgt gaacccttag
     7561 cgatcgaatc agcggtaaaa aaagcttctg ctccaaagat tgttatttat ggattggttg
     7621 gaatctttgt cgcattgatt ctgcaaagca tcgctgtcat gttagaatct gtattgtttg
     7681 gcaaagcaac cccttccgag aatacacaaa atatcatcca gatgatcatg gaagcacccg
     7741 cttttatttt ggcgacaact gttgctggac ccatcatgga agagttcgta ttccgccgca
     7801 gtatactggg gatcatcagt cgatattcaa atttttgggt cggagcagtg atcagctctt
     7861 tgctgtttgc gtttgctcat aatgatggtc atctgctaat ctactttttc ctaggtttct
     7921 tcttcagcct agagtacaaa gcaactggtc ggatctggac atcgatgatc acacacgtag
     7981 gaatgaacac attagttgtt cttgttcaat tagctgtcca aaaagggcta gtataaacat
     8041 gactataaat actcctgtca tcctagattg gtgattttcc caatctaaag cgacaggagt
     8101 atttttgttt tttagtcgtt catcttttct gctgctccat cattttttgt ctgtctttcc
     8161 tttttgcagt ttgcagaatc tatttttttc aaaaatggga aataaatgaa aaaactaatt
     8221 gacaacacga taccttgtaa aatcgctgca ggcactccgc ctactaagaa acccgagagg
     8281 atcggcggtg ttgtccaagg gacttggact gctgtaaata aaggaacgaa tccaaaatat
     8341 aaagcggaat aggtaatcaa tcctgaaacg accggtgcga aaataaaagg aacagccatc
     8401 atcgggttca tcacgatcgg tgtggaaaaa ataattgatt cattgatgtt gaaaaaggct
     8461 ggtaaaagag caagatttcc taattctctg tatttcttcg atttcgcaaa aaccgtcata
     8521 aaaaatacag cccctaacgt caatccagca cccgtaaccg tcatgaactg atcgagaaat
     8581 tgctgagtaa cgatatgtcc gccatttgcc accgtcaact cttgtcctga ggctaagatt
     8641 tctgcattcg ctaatgaatt cgcttgcaag acgggactaa ccaacccatt gaccatcgca
     8701 gatccatgaa gaccaaataa ccataaaaac ggaacaaaaa aacctaataa aagcacacca
     8761 aacaacgtgt ctatgaacga ttgaatggga agctggacat atgtccatac tccttgaaca
     8821 acagtcattc tgaacaactg ttgaaaaacc gctgaaccga taagcgtaaa agtgactata
     8881 acaatgattg gaatcaatga agtaaaagac tgccaaacat tttcaggtat cttctctctt
     8941 tgcttccacc ctttcgcaag tcttactaaa cgtgcatagc cccatccgat gatgagccca
     9001 gttgtaactg ctcctagtat cccttcagca cctgtccaga cagtatctat ctgagtattt
     9061 agtggctgaa taagcagtag ataagcagac aaggctgtta tgccgactgg taagcgatcg
     9121 atgtcttctt cttttgcata agcgtatgca atggcaaaaa cgatccaaaa ggctatcaca
     9181 tcaaatgtag cctggtatac ttgtgtaaaa tacatagaaa atcctgtttt gtctatccac
     9241 tgagcgatgt aatccacagg aaaatgagca aataagaaaa acagcgagcc aacaataatt
     9301 attgggatcg tgtaagtcat tccattacgc aaagcagtta agggtctaag attcacaaca
     9361 gatgttatca ttggtaaaaa ggcacgatct aaccactctt tcatttctta tctccttcaa
     9421 cttctatttt tatcaatcag ttttcttaat ttaactatac ccaatgatca cgaaccatga
     9481 aaggaatgcg cactgagctt ttcaaaaatc ggattctttg acgaatattc agcaactgct
     9541 tacaatggac aggaataaaa aaggaggttt tttatgaaaa ttgcactcat tggagctact
     9601 ggccatgcag gttcatttat tttagacgaa gcattgtcca gagatttaga cgtgacggca
     9661 atcgtgcgtc atccagaaaa attgccatca gatgttccgt ttatacaaaa ggatttattt
     9721 gagttgacaa ctactgatct tgcatctttt gatgttgtcg ttgatgcttt caacgctccc
     9781 aaaggaaaag aagaaatgca tcaaacttct ttgtcccatt tgattgagat gcttgaagga
     9841 accgatacac gcttaattgt cgttgggggt gcctcttcgt tattcttaga tacagaaaag
     9901 acgactcgta tgattgacca agtacctaaa aatgcaccct tctatccaac agctttcaat
     9961 atgtacgaag gattgttaaa attgaaacaa tctaaaaatc tacattggac ttatatcagt
    10021 ccagcttctt tatttgatcc acatggcaaa cgtacaggta cttatttgtt aagtgatgat
    10081 tatttacaaa gaaacgctgc tggcgacagt acgatcagca tggcagatta cgcgattgca
    10141 ttagtagatg agatactgga tgagaaacat gaaaagcagc acatcagtgt agtcagtcga
    10201 taatacggtt atttttttaa tatgctaaaa aaataaaaat agaagataaa accaaccctg
    10261 attgatttta tcttctgctt tttatatttt tattgattga actgtttaaa tcgaaaatag
    10321 ctcagttgct tcatctggat ctgtatcata gatttcaaaa tcatgtccaa cgccaaaatc
    10381 tttttctttc aaaacatttt ccattgttaa atgagcaagt tctttcatat tgttttcctt
    10441 gctaagatgg ccaagataga ttcgtttggt ccgattaccg atcacatcag tcatgacaag
    10501 tgcaccgtct tcgttggaaa gatgtcctct atctcctaaa atacgctgtt ttaaattcca
    10561 cggataaggt cccatgcgta acatctcaag atcatgatta ctttccatca aataaccatc
    10621 ggcattttca atgacacctc gcatataatc actgcaatat ccggtgtccg tcaacatgac
    10681 gaatgaccga ttttctttat ggaaacgata aaattgcgga gccgctgcgt catgggagac
    10741 gccgaaactc tcaatatcga tatcgccgaa tgttctgact ttccccatct caaatagatg
    10801 tttttgttcc actggtacat ttccaatcag tggttccatc gcttcccaag tctgctgatt
    10861 ggcaaaaaca tccaaatgat acttacgagc caagacaccc acgccatgta tatgatctcg
    10921 atgttcatgc gtcacaagta ttccatctaa ttggtccggt gtccgatcaa tttgagccat
    10981 caaggaagtg atttttttac cgctaagtcc tgcatcgacc aaaagccgtt ttttttcact
    11041 ttcgatataa agtgaattac ccgaacttcc acttgctagg atactgattt taaaagctgg
    11101 tttttcttca gccatttgac tcctacttcc cttcttactt tgtaaacttt tttcattata
    11161 ctcgcttttt tattgatctt ccacttttgg tacagtattg tttgtcacga tcgtattgct
    11221 gaaagcgttg acactctcaa tttggaccac tttatcgctt gtttcgatcg ctacatgcca
    11281 aacaggaaca tacacatttt tttcccggac tttcagaata caggaataag ctagctgacg
    11341 ccataaaatt tttgatcctc ttggtatttt gttattgatg tacaatgtat tgattgcttc
    11401 ctcttccgta tacagttccg tcttttcgcg cagctgctcg atatcagata aatgcgtctg
    11461 catgtatttc gtgatgctat aatcattatc gtttttttct aaagaaagaa cgatttttgc
    11521 tgtatcatca ttgaatggga tgccttcata ctcttggctt gcttggattt ctagtggttc
    11581 ttcttctaaa tgagaaaagt ttttcgtgta cacataatca ttaccgaata aaatattgtc
    11641 tttgttagat aaaaaatctt ccaaggaact actaatttta tctggatcga tcacgttctt
    11701 ttcagcatct gaactggata aagatttcgt caaaacatca ccgtctaaaa acacaccact
    11761 tgccagcaag ccgcttttac cagccttgcg ataattcgct aatgaagtgc taagattggt
    11821 ctgctccgcg ctaagataat agccttgctt gttctcagac gaaatcttcc ccgtgtaagt
    11881 gatatcgtcg ctagccagac gttgttggat tgattcagtc tgattagagc gaatgacatt
    11941 gttttcttgg tataccgcgt catgataact gctgaataaa aacacattca accctaaaaa
    12001 agcgacgaaa aatatccatt cgattttttt gaaatccaat taattcgcct ccaatccagg
    12061 taatttttcc agaaggtcgt tagctgaata ccagttatct ttatatttca cataccattc
    12121 aggtaagaga tcaacgacac gattcgtttc tttgacattt tgccatgtat agccaacgat
    12181 cactgaacca atcttgttgc tatctgctcc cttagctttt aactgctgga tgatttcatt
    12241 cgtacttgca agttctactt cttcgtctga aggtatcggc acttgaatcg tattcaaact
    12301 ggtttggata ttcacttgca cagaaccatt cgtttcaccg ccaacttcca caccaatctg
    12361 tcctctgctg tctgtcccaa aaaccgggaa gccttccacg aatatccgat aatcgatcgt
    12421 attgtcactg cggtcaaaat atctgaggtt ccctaaagaa ctgcctaatt tgctaatata
    12481 ggggaaactt tcagaaaata cactagcatt ttcttttttc tctggcagct ctccacgaaa
    12541 atcaactact tgcgtttccg aatggataga cattgtttca ttcccaccgt aaaaaatcaa
    12601 ttcattgctt tcatcattat ttttcacgct atctggtttc tggaaaaaag catttctaaa
    12661 aagtgtatag ggttgctgtg acaggatata actatacttt ttcagtttga tcgaatgttt
    12721 cgtagcatat tgcgtgtcga tcgtcgatgg ttctgttgtc atttctgtcc attttgtgtc
    12781 ttttgctgtt aattctttat tgatatctgt ccaattcaac gaaatatttg cttcatatac
    12841 gagatggttc gaataattca aaaatcggat ttttttattt ccttgatcga attgtattct
    12901 agtaaaatac atattttcgc tttcttttgc caagttcgac acatctaaat ctaaatcaaa
    12961 tgcttccttg tattctctta gcaaaaaagg cgcattatag gccaactcaa aaccagagtt
    13021 cagatttttc acctgttcaa actgtgattt gtctcctgaa actacttcag aaagctttcc
    13081 aaatcttgct ttatcgatga tcaattgcaa tcggtacacg agattttcac tgttggtttc
    13141 ttggatcatt cccgaatggt gccatgtaac atgaagcgga agaaagactt cggaagtttt
    13201 tcgattgctt tgagactcga tcatttgagt gtttgtctca tctacgttga ttgaactttt
    13261 tcctgctggg ctcagccaga ttgcataaga gaagtatatg cttaacgcaa ccatcaaaat
    13321 gagtcctata cgaacgactt tttcagagat tctcattccc accattcctc ctcataaggt
    13381 tcatatggca gcgagatata aaaagtcgac ccttttcctt ccttgctctc tgcccaaata
    13441 gcgcctcgat gcgccttaat cacttctcta gaaatagcta atccaagtcc tgtcccacct
    13501 tgtgctcttg cacgtgcttt atctactcga tagaaccgct cgaacactcg gttcagatct
    13561 tttttaggaa tccccaatcc ttgatcggtg atacttaaaa ccacgttatt atgcgtttct
    13621 aataagcgac aagtaatcgt tcccccatct ggtgaatact tgatcgcgtt attcatgata
    13681 ttatcgacta cttggatgat tttatctgta tctacttcga cccaaagatc tctttgcgta
    13741 aattcacgac ggatcttata atttttttcc tgatttccca ccatcatatc aaaccggtca
    13801 agaacaaaat tcactaactc gttgaaattg acatattcta attgaagctg ggtattacct
    13861 gtatccatgc gagatagatt taagaggtca ttgatcattc gaatcattcg gtctgtttca
    13921 tctagcgtta ccttcaaaaa atttggtgct acttcctcgt ctttccacgc accttcgctc
    13981 aatgcttcga tataactacg catacttgtc aacggtgtac gaagttcatg ggaaacatta
    14041 gagacgaatt cacgacgttc ccgttctgtt ttttcttgtt ctgtcacatc atgcaagaca
    14101 gctactaatc cgctgataaa acctgattcg cgacggatca tagaaaagtc tactcgcaag
    14161 atcatctgat cactttccaa tgtactggtt gaacggtcaa gtaccatttc gtttggatct
    14221 tctaataatt tccgcaacgt atattcttct tcgatatcta aaagtgtcaa aatcgattgt
    14281 ccgattgctt cctcgttttt gatatctagc aaggaaacag ccatatcatt gatcgtaatg
    14341 actttacccc tgcgatctgt cgcaatgacg ccgtctgtca tatgggatag cacgctatcc
    14401 aaacgattgc gttcagcttc cattgtatct tgagcttctt caatacgttc agctaactga
    14461 ttgaaggtat cagccagctg tccaagttca tcttgtccat gcactttgac tttccgactg
    14521 tagtctcctc tagcgattcg aatggcttgt tctctcattt caccgatcgg ctgtgtaatc
    14581 gaacgagcaa ctaaaacaga tacgatcatt gaaataaatg ccgcaattag tgaggcagta
    14641 aagaaaatga ctgctgtgtt attgatttcc gaatattttt gttcgatatt acttttgacg
    14701 taaagagccc cgataacggc atcgccggtc ggggacagga tcggttgaac attgatattc
    14761 acccgtttat cgtcgttatc taaagcaaca taacgtttag tcgtgaaatc attgatatca
    14821 acataatcat ttttctttcc cacagcgctt tgttcattga catcggttgt tcctcggatg
    14881 atcccttttt cgtcaacgac ccgtatttcg atgatatcgc tagtcgcact gttttcaata
    14941 aatcttttga tattcgcatt tacctcttca tcatctgcat tatcttttcg attcaattcc
    15001 gggctgattg ttgttgctaa tgattccacc gtttgattca tatctttaat aaatgtattg
    15061 atcgtggaac gttctaaacc acgtataaag taggcaccaa taatttcgat cgagatcaat
    15121 aaaatcagaa taaaggtaat agcgatctta aaattgactg atcgaaaaaa gcggactttt
    15181 cttttcatcc aattactcct gttctgggtt acgcaaatag taaccgacac cacgacgtgt
    15241 cactaaataa tttggatggc ttggattatc ttcgatcttt tcacgcaaac gacgtacagt
    15301 tacatccact gttcgtacat ctccaaaata atcataaccc caaaccgttt gtaataaatg
    15361 ttctcttgtc atcacttgtc caaggtgctt cgccaagtaa aagagtagtt cgaattcacg
    15421 atgtgttaac tcgattgttt ctcctcgttt cgtcaccatg taagcttctg gatggatcgt
    15481 caaatcacca atcgtcaatt ccgcaggtgc tgcctcttct ggttccttcg cagccgtggc
    15541 accgcgacga agatttgctt tgacacgtgc aactaattct cggttggaaa atggcttggt
    15601 cacgtaatca tcggcgccta actctaagcc taggacttta tcaatttcag aatctttggc
    15661 tgttaccata atgatcggca tatcataagt tttgcgtacc tcacgagcaa cttctaagcc
    15721 gtccatttta ggtagcatca aatcaagtaa aatcaaatca ggttctacct cagcaacttt
    15781 ttccaaggct tcttccccat catatgcagt ataaacatca tagccttctt tggctaaatt
    15841 gaatttgacg atatccgaaa tcggcttctc atcatcgaca actaaaactt ttttcataaa
    15901 gagccacctc ttatatatta ttgtcatacc ttctcattat accgtatttc tgcttttgtt
    15961 tcatctttgc ctataaaaca aaagagaaaa ttaaaatgaa agtgcaaaat actttattat
    16021 tttttgttct aacttcaata aaaaaggaaa acactgattt tcacctcagt gtttcctttt
    16081 tttgatttat ccttctaaaa cttgtacacc gacacccaat aatcccggac cagtgtggac
    16141 tactaatgcc ggagaaatct ggccaaagta gatttggttt gcttgtggga actctctttt
    16201 aagtctcgct tccatttcaa ttgcttcttc tttcgcatcc ccatgggcaa cagctaagtt
    16261 gaagaccttt gcattaccga ttttttcttt gacgtaagca accgttttat ccaaactttt
    16321 tttacgtccc ctagcttttc ctactgtgta ataaatccct tcttcattac atgagatgat
    16381 tgggttaagc tttaaagcgg ttcctacaat agaagcaact aaaccgatcc gtccaccttt
    16441 ttgaagatat tccaatgtcg caacattaaa gaaaactttt gttctttcga ctaactcatt
    16501 cagattactg atcagttgat cccattctac tccttcttcc agccattttg ctgcttggat
    16561 cgcagaaaaa cctgcaccaa taccaatatt tttcgtatct agtacatatg cttccaattc
    16621 ttcttgctgt tgtgcaatta aacgaaccac attatatgtc ccgctcaatc cactggaaat
    16681 tgttaccgca agtacctttt cataaccgtc ttttttgatt tgctcaaaga tttctgtgat
    16741 cgactcacca tccggcaaag atgtaccagg gatttcctct ggcaaacgtt gatagatctc
    16801 ttcagaggta atgtcaattt tatctgtata tacctgatct ttataaataa ttttcaatgg
    16861 gatcatatac atctcgtatt tttttatcaa ttcttctggc acatccgtac ctgaatctac
    16921 taaaattgcg attttattgt ttttcatttg ttttctcctc ttttaaatat tcggtttcta
    16981 gaacaatcgt tttttccgca agaagtttca ttgcaaacga taaagttgcc gctcttacag
    17041 caatatattc tacaggggtt gcctggtcga atttttgcgg atgagattta ccttgtgcta
    17101 attggctgac catccacaca gctgcttctt gttcatcgca aaatagatta taggcttcac
    17161 gaattcctac tgtttttcct tggaacaata tcccttcttt gatttcagga atagtcaata
    17221 cttgtttcaa cgtagtgatt gcaagtaaaa atgctacgtg ttctttgtta tatctttttt
    17281 taactggtgc tggtatcaaa cctagtttaa cgtaattgtt taccatagaa gaggtaagca
    17341 atggatgctt ttctccttgt atcacaggtg aaagataacg atcaactaac gttctgactt
    17401 ggtccatata aagttctaag tcagggagtt cttcccagtg aggcagacgg aatgtttcta
    17461 gttccttccc ccactccatt aattgctgac gcattttttc catcataacc gctccttttt
    17521 tcatttcata aacatgatat cagacttatg ataaacattc aatctagttg ctttaactag
    17581 gtgatttgat tagcgggatt ccatgaaaac aaaaaacaag aaggctaagc cttcttgtct
    17641 tttatcgtta agaacaggag acccgccggg gagacggcgg gaaaggagta aaaaatgaaa
    17701 aaagtgttag ggtttgttct cttgcaagaa ctttgtttgt tggtatgctt atataatagc
    17761 gagcaaatgt gaataatcta tgaatgattt cctataagga agtaaagatt tttctgattt
    17821 tctttaaatg tagtcttgca ttttaaaatt cttagtgcta ctattattaa gtactttttg
    17881 tacgggccgt tagctcagtt ggtagagcag ctgactctta atcagcgggt cgcgggttcg
    17941 agcccctcac ggcccattgg gtgccaaacc cacggaacag gtaaatgttg gtgcgaaagc
    18001 atttcgaaaa catttacgct gttctttttt tattt
//
LOCUS       DASJTC010000059        17740 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.059, whole genome shotgun sequence.
ACCESSION   DASJTC010000059 DASJTC010000000
VERSION     DASJTC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.059"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            29..841
                     /locus_tag="U4B19_001825"
     CDS             29..841
                     /locus_tag="U4B19_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEN2077560.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            838..1638
                     /locus_tag="U4B19_001826"
     CDS             838..1638
                     /locus_tag="U4B19_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEN2077561.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1799..3067
                     /gene="rseP"
                     /locus_tag="U4B19_001827"
     CDS             1799..3067
                     /gene="rseP"
                     /locus_tag="U4B19_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEN2077562.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3135..4844
                     /locus_tag="U4B19_001828"
     CDS             3135..4844
                     /locus_tag="U4B19_001828"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEN2077563.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5056..9408
                     /locus_tag="U4B19_001829"
     CDS             5056..9408
                     /locus_tag="U4B19_001829"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEN2077564.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9552..10025
                     /gene="rimP"
                     /locus_tag="U4B19_001830"
     CDS             9552..10025
                     /gene="rimP"
                     /locus_tag="U4B19_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEN2077565.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10049..11224
                     /gene="nusA"
                     /locus_tag="U4B19_001831"
     CDS             10049..11224
                     /gene="nusA"
                     /locus_tag="U4B19_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEN2077566.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11246..11539
                     /locus_tag="U4B19_001832"
     CDS             11246..11539
                     /locus_tag="U4B19_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEN2077567.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11536..11847
                     /locus_tag="U4B19_001833"
     CDS             11536..11847
                     /locus_tag="U4B19_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEN2077568.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11860..14166
                     /gene="infB"
                     /locus_tag="U4B19_001834"
     CDS             11860..14166
                     /gene="infB"
                     /locus_tag="U4B19_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEN2077569.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSVEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14193..14540
                     /gene="rbfA"
                     /locus_tag="U4B19_001835"
     CDS             14193..14540
                     /gene="rbfA"
                     /locus_tag="U4B19_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEN2077570.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14648..14869)
                     /locus_tag="U4B19_001836"
     CDS             complement(14648..14869)
                     /locus_tag="U4B19_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2077571.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15317..17680
                     /locus_tag="U4B19_001837"
     CDS             15317..17680
                     /locus_tag="U4B19_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEN2077572.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6048 a         3147 c         3908 g         4637 t
ORIGIN      
        1 gaatctttta agtataggag gaaaaagaat gttacgtttt tttccgcaaa aaaacaaata
       61 tattcaagaa aaaagcgaat ttcaatttaa taccgatgga ccaattcctc agcacattgc
      121 catcatcatg gacggcaatg gtcgttgggc gcaaaaccgt cgtttgccac gagttgctgg
      181 ccataaagaa ggcatggaaa cagtaaagaa agtcacaaaa aaagcatcaa ggcttggagt
      241 gaaagtgttg acgctttatg ccttttcgac tgaaaactgg aagcgaccaa aagatgaagt
      301 aagttttctc atgcaactac ctgtcgactt ctttgatacg ttcgtaccag aattgattaa
      361 agaaaatgtc aaagttcatg tcatgggtta tgaaaacgta ttgccagaac atacgcaaga
      421 tgctgtaaga cgtgcaattg agcaaacaaa aaacaatact ggaatggtat tgaattttgc
      481 tttgaattat ggaagtagag cagagatcgt gactgctgtc caagaaattg ccgaagaagt
      541 agcaaaagga gaaatacacg cagaagaaat cgatgatgaa ttgattgcta agcatttaat
      601 gacaggtttc ttgcctaaag agttacaaga tcctgagctg atgatccgta caagtggaga
      661 agagcgaatt agtaattttc tgttatggca gatcgcatat agtgagctgt attttacaaa
      721 agcgctttgg cctgattttg atggtgcgca tcttgaagaa gcaatcgcct cttatcaaaa
      781 tcgagaccgt cgattcggcg gtgtaaaaaa aacagaagat gagaaagggg atcagtcatg
      841 aaacaacgtg tgatcacagc agctgtggcg ctagtcttgt ttattccgat tatatggatc
      901 ggaggcatag cggttgagct tacagcagca atcttagcgg tagtaggagt ttacgagctg
      961 ttccggatga aaggattgac actcttaagt tttgaaggag ttttatccgc cgttggtgca
     1021 gtgttacttg ttttgccaaa agaaagatgg tttttctttc ttccagagaa tgtaagtaca
     1081 tttatgttat tttatcttat agttatgatt cttttaggaa catcagttat ttctaaaaat
     1141 acgtatacga ttgatgaagc aggttttcct gtgcttgtca gcttgtatgt gggaataggt
     1201 ttccagaatt ttgtcaatgc acgaacagaa agcttggctg ttttgatatt tggtctattt
     1261 attgtttggg caactgatat cggagcatat atggttggtc gacgttatgg tcagcataag
     1321 ttaagtcctg atatttcgcc caacaaaacg attgaaggtg cgctcggtgg tatcggtagt
     1381 gctgttgtag tcgcatttct ttatttattg ctatacccag caaaagaact attcaatcat
     1441 ggtatgtttg tcatgcttct gatgacgatt attttttcag cagtcggaca atttggtgat
     1501 ttggtcgaat ctgcaatcaa acgtcattat gatgtgaaag actcgggaaa tatcttacca
     1561 ggccatggtg gaatcttaga ccgtttcgac agtttgctgt ttgtatttcc tatcatgcat
     1621 ttatttggca ttatttagta agtatacctg cgtttctttg tgaatttcat aaaagacgca
     1681 ggtttttatt tttcattata ttgatactat tggtgtcttt ggtatactat ggtaaaatta
     1741 tagtgtttta tcgttgtttt ataaaaaaca caaaaaacaa gtaaaggaga aaaaagctat
     1801 gaaaacgatt ctgacattta tcatcgtttt tggtatatta gtgattgttc atgagtttgg
     1861 tcatttcttc tttgcgaaac gatcaggaat cctcgtccgc gaatttgcta tcggtatggg
     1921 tccgaaaatt tatgggcatc aagcaaaaga cgggacaaca tatacgcttc gcctattacc
     1981 tattggcggt tatgttcgaa tggcaggcaa tggggatgat gaaacagaaa tggcaccagg
     2041 aatgcctctt tctcttttac taaattctga cggcatcgta gagaagataa atttaagtaa
     2101 gaaaatccaa ttgacaaatg ctatcccgat ggaattgagc cgttatgatt tagaagatga
     2161 gctgactatc actggttatg tgaatggaga cgaaacagaa gtagtaacct accctgttga
     2221 tcacgatgcg acgattattg aaaacgacgg aacagaaatc agaattgcgc caaaagacgt
     2281 acagttccaa tcggctaaat tgtggcagcg tatgctgacc aactttgctg gacccatgaa
     2341 taactttatt ttggcaatcg tgttattcat cattctggca tttatgcaag gtggtgttca
     2401 agtgacgaac actaatcgcg taggagagat catgcctaat ggagctgcgg cagaagcagg
     2461 cttaaaagaa aatgatgagg tagtcagtgt ggacggaaaa gaaatccatt catggaatga
     2521 tctgacaaca gtcattacga aaaatccagg taaaacatta gactttaaga tcgaacgaga
     2581 aggtcaggtg caatctgtcg acgtgacacc gaaatctgta gagtcaaatg gcgaaaaagt
     2641 tggccaactt ggtataaaag ccccaatgaa tacaggtttt atggataaga tcattggagg
     2701 gacgcgtcaa gctttcagcg gttcattgga aattttcaaa gccttaggat cactatttac
     2761 aggtttcagt ttagataaat taggcggacc tgtcatgatg taccaattgt cttcagaagc
     2821 agcaaatcaa ggtgtaacaa ctgttattag tttgatggcg cttctttcaa tgaatctcgg
     2881 aatcgtcaat ctgcttccga ttcctgcctt agatggcggg aaattagtct tgaatatttt
     2941 tgaagggatt cgcgggaaac cattaagtca agaaaaagaa ggaatattga cattagctgg
     3001 ctttggattt ttaatgttgt tgatggtgct agttacatgg aacgatattc aacgattctt
     3061 tttctagaaa aagttgacaa atagggtata ataacaaacg ataactattt tgaggaaata
     3121 aaggagtata tagaatgaga caatcaaaaa tgttgattcc aacattacgc gaagttccaa
     3181 atgatgcaga agtattaagc catcaaatct tgctaagagc tggatatatc cgccaagtat
     3241 cagcgggtat ttattcatat ctgccattag caaatcgtgt attggaaaaa ctgaaacgca
     3301 ttatgcggga agaatttgaa aaaatcggag cggtagaaat gctgatgcct gccatcttgc
     3361 cagcagaact ttgggaagaa tccgggcgtt acgaaacata tggaccaaac ttgtatcgtt
     3421 tcaaagaccg caatgatcga aaaatgatcc ttggaccaac tcacgaagaa acttttactg
     3481 atttgatgcg taatgaaatc aattcatata agaaattacc tttaaatctt tatcaaatcc
     3541 aacctaaata tcgtgacgaa aaacgacctc gcttcggttt gcttcgcgga agagagttta
     3601 tcatgaaaga tgcatattct ttccatgcaa cggaagaaag tttggacgag acatataaag
     3661 attacgaaaa agcctatacc gaagtattca agcgttgtgg cttagaattt cgctcgatca
     3721 tcggagacgg cggcgcaatg ggcgggaaag actcaaagga attcatggcg atctcagaaa
     3781 tcggggagga tacgatctgt tattcgactg aaagtgatta tgctgcaaac ttggagatgg
     3841 ctacaagcta ctatgtatca aaaaaatcgc atgaaacaca gcttgaattg gagaaagtag
     3901 ctacacctaa cgtaaaatca attgacgaag tagcagattt cttttctgtt gaacctcaaa
     3961 aaatcatcaa gtctgtattg ttcattgcgg atgaacaacc tgtccttgtt ttggtacgtg
     4021 gagaccatga agtcaatgac gtaaaactga aaaacttttt atctgctgac tttttagaag
     4081 aagcaacaga agaagaagca caaaaatatc taggtgctga gtttggttct gtcggaccag
     4141 tcggtgtttc agatgacgta aaagtatatg cagaccgtta tgtccaagat ttagcgaatg
     4201 ccattactgg agcgaacgaa acaggattcc actataaaaa tgtgaatcca aaacgcgatt
     4261 tcactgtgct ttcttatgaa gatcttcgtt ttgtacaaga aggagatcct tcaccagaca
     4321 acaacggtgt tcttgccttt actcgaggaa tcgaaatcgg acatatattc aaaatcggca
     4381 ctcgttacag tgaatctatg ggagcaactg tactagacga aaatggacga gaaaaatttg
     4441 tgattatggg ctgttatggt atcggtgtaa gtcgtttatt atcagcaatc gtagaacaac
     4501 attcagatga acaaggaatc aattggccaa aaggaattgc gccatttgat ctgcatatcg
     4561 tccaaatgaa tctaaaagac gaataccaaa caagcctgac gaatgaacta gaagaagcga
     4621 tgacaaaagc tggctatgaa gtactggttg atgacagaaa tgaacgcgca ggagtgaaat
     4681 tcgcggattc tgatttgatt ggctgtccaa tcagaatcac tgtcggtaaa aaagcagtgg
     4741 atggaatcgt agaagtgaaa atcaagaaaa ctggcgaaat ggttgaagta cgtaaagaag
     4801 aattggcgaa tactttacca atcctcttga accaaggcga ataataagcg ttaaaacaca
     4861 gaaaataaaa atagctgaat cagctgtttt tgacaatctg atgattcgct tttaaaattg
     4921 gaactgcgat ttgaatcgca gttccaattt tttaatgtaa aactcagaaa aaacgagtat
     4981 actaaaaggt actgaaaaag aagaggataa gatgcgatca attgcattgt ttctcatcag
     5041 aaagggcgta tatctttgtc agaaaaacga gatttatttg aaaaactgtt ggaacaaatc
     5101 caactagagg aagcagaaaa aaatcatcca ctgcttactg ctggtgaaat ggatagtgta
     5161 gtggtccatc gtaaatcgcg tttatgggaa tttacgctac atttttccaa aattttgcct
     5221 attatgctat atcggtcatt gactcagcat ctgactatcg cttttaaaga tattgcacaa
     5281 gtcaaactga atatcttagc agatgaccag acattcgatg aacagctctt gcaagattac
     5341 tgggcccaag ctttggaaaa tcagcaatgc gatacaccac tagcacagca agtattgaaa
     5401 acgcaggtac ctgtgataaa agatcgtaaa gtcatcttgc caatcgacag tacaggagct
     5461 atttcttatt tgaagcaaca atacttgcca ttagtagaag agttgttcgt cagttacgga
     5521 ttcccaaaat ttcgtatcga accagaagtg gatgagcagc aagcggaacg tgtattgaaa
     5581 ctttttgaag aacgcaaaca agaacaggca gaagccttta tgaagcaagc agctgaaagt
     5641 ctagttgtcc acgaacaaaa gaaaaaggaa agaaaagagc aatcgccagc tcttgaagga
     5701 cccatccagc taggacgaaa tattccagcc gatgaaccaa cgacaccaat gatcagtatc
     5761 gttgaagaag aacgaagagt cacacttgag gggtacgtat tcgacaaaga agtcagagaa
     5821 ctacgttcaa aacgcaagat cttgactttg aaaatcacag attatacttc atcatttatc
     5881 gtcaaaaaat tctcgaacgg cgaaaaagat gaacaagtat ttgatgcgat cagtgtcggt
     5941 agctggttaa aggtacgagg aagtattcaa gaagatacgt ttgtccgtga tttggtcatg
     6001 aatgcgcaag atatcataga agtcaaacat acacctcgca aagattatgc tcctgaaggg
     6061 gaaaaacgag tagagcttca tgttcatagc aatatgagta cgatggatgc aacaaacagc
     6121 atctctgatc ttgtggcaca agcaggaaaa tggggacacc gagctatcgc cattacagac
     6181 catggtggtg cgcaagcctt tccggaagca catagtgctg gaaaaaaagc aggtgtgaaa
     6241 atcttatacg gcgtggaagc gaatgtcgta gatgatggtg tcccaattgc ttataatgat
     6301 gcacatgaag cattgagtga agccacatac gtcgtatttg acgtggaaac gacaggattg
     6361 tctgcagtat atgacacgat catcgagcta gccgcagtca aaatgtataa aggaaatgtg
     6421 atcgaaagct ttgatgaatt tatcgatcca ggacatccat tgtcacgaac aacggtagat
     6481 ctgactggta tcacggatga aatggttcga gggtcaaagt cggaagaaga agtattgcgg
     6541 atgttccttg aattttcaaa agatacaatt ttggtagccc acaacgcggc ttttgatatg
     6601 gggttcttga atactagcta cgcaaggtat ggtattcctg aagcggccaa tccagtcatt
     6661 gatacgttgg aattagctag atatctgtat cctcaattca aacgtttcgg attaggtgtg
     6721 ttatctaaaa agtttggtgt tagcttggaa cagcatcacc gagcaatcta tgatgctgaa
     6781 gcaactggtc acttagcatg gatctttgtg aaagaggcaa tggacaatca taacatgctt
     6841 tatcatgatc agctaaatga gcatatcgga gaaggagatt cttacaaaag ggctcgacct
     6901 ttccatgtaa cgattttagc aaaaaatcag gcaggactga aagatttgtt caaactgatt
     6961 tcaatgtcga atgtcgaata cttcgaacgc gtcccacgaa taccacgctc ccaattgaaa
     7021 aagatgcgtg aaaatctatt gatcggctca gcttgtgata agggagaaat attcgaagcc
     7081 atgatgcaaa aaggggtaga agaggcaaga aaccgagcaa aattctatga ttatattgaa
     7141 gttatgccaa aagcggtcta tgcgcctttg atcgagcaag aattggtaaa aaatgaacat
     7201 gatttggaag aaattatcca aaatttggta gaaatcggaa aaagtttgga taaaatcgtt
     7261 gttgcaacag gtaatgtaca ttatctgaac gaagaagatg ccatttaccg aaaaattttg
     7321 atcaattcta tgggcggagc gaatccgttg aatcgtcata gtttgccgga tgtccatttc
     7381 cgaacaaccg atgagatgct aactgcattt cactttttag gggaagaaac agcgaaagag
     7441 atcgtagtag aaaatacgaa taaaatcgca gatatctgcg aagaagtgat tccagtcaaa
     7501 gatgaacttt atacaccgaa gattccaggt tcagaagatg aaatatcaga attgagttac
     7561 acaaaagcaa aacaaatgta cggagatccg ttacctgaga tcattcaaaa acgattagaa
     7621 aaagagttga attctattaa tggtaatggc ttctcggtta tttatctgat cgcccaaaaa
     7681 ctagtgcata aaagtaatga agatggctat ctagtaggtt cacgtggctc tgttggctct
     7741 agctttgttg cgacgatgac aggaatcact gaagttaatc cattagcccc tcattattat
     7801 tgtccagagt gccaatattc cgagtttttt gaagatggta cgtatggttc tggatttgac
     7861 atgccagaaa aacaatgtcc aaaatgtggt gcacgcttaa acaaagacgg tcatgatatt
     7921 ccatttgaga catttttagg attccatgga gacaaagtgc ccgatattga tttgaacttc
     7981 tcaggtgatt atcaagcaga agcccataac tatacgaaag tattgtttgg cgaagactat
     8041 gtttaccgtg ccggaacgat tggtacagtc gccgacaaaa cagcatatgg ctatgtaaaa
     8101 ggatacgaaa gggacaataa tctgcagttt cgaagtgcag aagtcgacag attagcaaaa
     8161 ggcgctactg gagtcaaacg gacaaccggc cagcatccag gggggatcat cgttattccc
     8221 gattatatgg atgtctatga ttttacgccg atccaatatc cagcagacga tcaaaattca
     8281 gaatggaaaa cgactcattt tgatttccac tcgattcacg ataatgtatt gaaattagat
     8341 attctagggc atgatgaccc aactgttatt cggatgcttc aagatttatc agggatcgac
     8401 ccacaaacga tccctacgga tgacccggaa gtgatgcgga tatttgccgg tcctgaagta
     8461 cttggagtaa gtcaggaaca aatttattcc aaaacaggga cattaggtat tccagaattc
     8521 ggcactcgtt ttgttcgagg gatgttagaa gaaacgcatc ctacaacttt tgcagaacta
     8581 ctgcaaattt ccggactatc tcatggtaca gacgtatggt taggaaatgc ggaagaattg
     8641 attcgacgag gagatgcgac actggcggaa gtaatcggct gtcgtgatga tattatggtt
     8701 tatttgatcc atgctggatt agatagcggg atggcattta aaatcatgga aactgttcgt
     8761 aaaggacaat ggaacaagat ccctgatgaa ttgcgagaaa cctatctatc ggcgatgaaa
     8821 gaaaataatg tgcccgactg gtatatcgat tcttgttcaa aaatcaaata tatgtttccg
     8881 aaagcccatg ctgcagctta cgtattgatg gctcttcgtg tcgcttattt caaggtttat
     8941 ttccctattt tgtattactg tgcatacttt tctgtacgtg cagacgactt tgatctagtc
     9001 tccatgtgca aaggaaaaga tgctgtgaaa caagcaatga aagaaatcac ggacaaggga
     9061 ttagatgctt ccgtaaaaga aaagaatcaa ctgacagtgc tggagttagc caatgaaatg
     9121 ctggaacgag gattcaaatt tggaatgatt gatttgtata aatcggatgc cgttaacttt
     9181 gttattgaag gtgatacgtt gatcgcacca tttagagctg tcccaagttt gggaaccaac
     9241 gtggcgaaac aaatcgttga agcaagaaaa gacggtccat ttttatccaa agaagatctt
     9301 gccacccgcg gtaaagtttc aaaaacattg atcgaatata tgaacgacaa tggcgtattg
     9361 aaagatctac cagatgaaaa tcaattatcg ttattcgata tgctataagc atctgttttc
     9421 ctgtctgctt gtaattttaa gcagagtatg gtatagttct tataggtaat gatactaact
     9481 gtgagtgagc ggatttttcg ctcactcttt ttaatggaat tacgcactta atttttaagg
     9541 aggcgaaact tttgagtagc gtcgttgaaa cagtcacaga aatggtgaca ccgattttag
     9601 atgaacaaaa ttttgaactt gtcgaagtcg aatttgtaaa agaaggaaaa agctggttct
     9661 tacgagtatt tattgacaaa gaaggcggta tcgatatcga agaatgcgcc tttgtcagtg
     9721 agaaactgag tgaaaaactt gatgcagcag atccagatcc gattcctcaa gcttatttcc
     9781 tggaggtatc ctccccggga gcagagcggc cattgaaaaa agaaagcgac tatgaaaagg
     9841 cacttggtga ttatgtccat gtctccttat accaaccagt agatggcgaa aaacaatatg
     9901 aaggatttct tcaatcattt gatgctgaac agcttacatt gaaaatacgc ataaaaacac
     9961 gagaaaaaga attgactttt gaccgaaaaa atattgcaaa agctcgctta gctatccaat
    10021 tttaatcacg gagaggaatc agaaaaaaat gagtaaagaa atgttaaatg cgttagacgc
    10081 tttagaagct gaaaaaggga tctcaaaaga aatcgtgatc gatgctttag aagcagcatt
    10141 ggtttctgca tacaaacgtc actatggcca agcacaaaac gtcgaagtgg aatttgatca
    10201 gaaaaaagga aagatccatg tctatgcggt aaaagaagta acggaagaag tgatggattc
    10261 acagttggaa gtctcattga aagatgcact tttaatcaat ccagcatatg agatcggtga
    10321 tacgatccgt tttgaagtaa caccaaaaga ttttggacgg atcgctgcgc aaacggctaa
    10381 acaagttatt ttacagcgtg tacgtgaagc agaaagaacg attatttata acgaattcag
    10441 tgcttatgaa aaagacatca tgcaaggaat cgtcgaacgc caggataaac gttatattta
    10501 tgtcaatctt ggtaaaattg aggcagttct ttctaaacaa gatcaaatgc caaatgaatt
    10561 ttatcaacca catgatcgaa tcaaagttta tgtttcccga gtagaaaata cgtctaaagg
    10621 tccgcaagta tttgttagcc gcagccatcc agacctattg cgtcgcttat ttgaacaaga
    10681 agtaccagaa gtctatgatg gaatcgttga aattgtcagc atcgcccgtg aagctggcga
    10741 tcgttcaaaa gtggcagttc gctcaaatga tccaaatatc gatgcagtcg gtacatgtgt
    10801 cgggccaaag ggacaacgag ttcaagcaat cgtcaatgaa ttaaaaggcg aaaacatgga
    10861 tatcgtcgaa tggaatgaag atccagctgt attcatcgca aatgcattga atcctgcaca
    10921 agtcatggat gtgatcttcg atgagacaaa tccaaaggcc tgcacagttg tcgttccaga
    10981 ctaccaatta tcgctagcaa tcggtaaacg cggacaaaat gcacgtttag ctgctaaatt
    11041 gactaaccat aaaatcgata tcaaatcaga atctgacatg acggaatttt atgaaaatca
    11101 agaacaacaa aaagaaacag aagaactgca tgatgaagca atcgttcagt cagatttaac
    11161 agacgatgaa tatgaaacaa ttgcattcaa caatgaaacg gtcgaagaaa aaccagaagc
    11221 ttaactttag taaggaggca aagagatgaa acaaaggaaa attcctttac gcaaatccgt
    11281 ggtttcaggt gaaatgaaac ctaaaaaaga gatggtccga atcacccgtt caaaagaagg
    11341 ggaagtatcg atcgatccaa ctggaaaaat gcctggacga ggagcgtatg tctcacttga
    11401 gccggaagaa gtacaacaag cttgggataa aaaaattctc gatcgagtac tcgaaacaac
    11461 attaacagat gagttttatc aagaacttct tgactatgtc actcaccaaa aagcacgtaa
    11521 agagttattt ggaaaatgaa tacagttaat cggcaaaaag ccatgaatct catcggtcta
    11581 gcgatgcgcg cgggaaaact aatcacgggt gaagaactga ccattgctga aatccgcaaa
    11641 caaaaagcaa aattggtgtt tgtggcaacc gatgcaagtg agaatacaag aaaaaagata
    11701 aaagataaga gttcatatta tgaagttcct tgctttgagc tattttctga agcagaaatc
    11761 acgcagatga tcggaaaacc tcgcaaggta ttcggaataa tggatgccgg atttgcgaag
    11821 aagaccaagg agctaattga aggttaggaa ggtgattgca tgggcaacaa aagaatctat
    11881 gaattagcaa aagaactcaa aaaatctagt aaagacgtcg tggaaaaagc acaacaactt
    11941 ggcatcgatg tgaaaaacca tatgggtgcg attactagca gtgatgagaa gaaattgcaa
    12001 caagcgttca aaaacccaag cacgaaacaa caagctgcac aacaagcaag cagaaaacca
    12061 gccaatcaac agccgaacga tcaacgtaaa aaagaaacaa cgaacaatca acaaaaaaat
    12121 aaaaataacc gcagttatca agatcgcggt caagggagcg gtcaagtgaa tcaaggaaaa
    12181 aaaccaacaa caaaccaaaa taatacggga aataccagca gccaaaatcg tcaaggaacg
    12241 agccaaggcg gcaatcaaaa ccgtactaac aataacaaca aaccaaacaa taacaatcgc
    12301 ggcaaattta ataataataa ccgtaatcgt tataataaaa aaggtaaaaa aggcaaacag
    12361 caacaatcaa ctaaaccagc tgtaccacca cgcaaattcc gtgaattgcc agatgtatta
    12421 gagtatactg aaggtatgaa cgtagcagat attgcgaaaa aaattcatcg tgagccagca
    12481 gaaatcatta aaaaattatt tatgcttggt gtcatggtca atcaaaacca agcactggat
    12541 aaagatacga ttgaactgtt agcgacggac tatggcatgg aaccacaaga aaaagtccaa
    12601 gttgacattg ctgatatcga taaattcttc gaggcagaag aagtcgatcc agataaatta
    12661 gtctcacgtc cgccagtagt aacgattatg ggacacgtag accacgggaa aacaacctta
    12721 ttggatacat tgcgccattc acgtgtaaca agcggcgaag ccggcggaat cacacagcat
    12781 atcggtgcct accagatcga tattgatggt aaaccaatca ctttcttgga tacaccagga
    12841 catgcggcct ttacaagtat gcgtgcacgt ggagcaagta tcacagatat cacgatctta
    12901 gttgttgctg cagatgatgg cgtcatgcca caaacagttg aagcaatcaa ccatgccaaa
    12961 gctgcaggcg ttccaatcat cgtcgctgta aataaaattg ataaaccagg tgcaaatcct
    13021 cagcatgtta tgcaagaatt aagtgagtat gaattaattc ctgaagcatg gggtggagaa
    13081 acgatttttg ttgagatctc agcgaaattc ggccaaaaca tcgaagaatt gctggaaatg
    13141 atcctgttag tcgcagaagt agaagattta aaagcagatc caacacaacg cgcaatcgga
    13201 acagtgatcg aagcacgtct tgataaaggt aaaggtcctg tgtcaacctt acttgtccaa
    13261 gaaggaacac ttcacgttgg tgacccaatc gttgtcggta atacttacgg acgtgtccgt
    13321 gtaatgacaa acgatcttgg ccgtcgcgat aaagaagcag gacctgcaac gccagtagaa
    13381 atcactggct tgaatgatgt acctcaagca ggtgaccgtt tcgtagtctt cgaagacgaa
    13441 aagacagcac gtgcagcggg tgaagaacgc gcaaaacgtg cattattgga aaaccgtgct
    13501 gccacaagcc gtgtgacttt ggacaactta tttgaaagcc tgaaagaagg cgaattgaaa
    13561 gaagttaacg taatcatcaa agcagatgta caaggttcag tagaagcact ggctgcatca
    13621 ttgaagaaga ttgatgtgga aggtgtccgt gtaaaaatcg tccattcagc tgtcggagca
    13681 atcaatgaaa gtgatgtaac cttagcagca gccagcaacg ctattatcat cggtttcaac
    13741 gttcgtccta caccgcaggc aaaagtgcaa gctgatactg aagaagtcga tattcgtctt
    13801 caccgtatca tctataaagc catcgaagaa atcgaaactg cgatgaaagg gatgctagac
    13861 cctgaatttg aagaaaaaat caccggtcaa atgatcgttc gtgaaacctt caaagtttct
    13921 aaagttggta cgattgcagg tgcctatgta acagaaggat atattcgtag agatagcggt
    13981 gtccgtgtaa tccgtgacgg aatcgtaatc tatgaaggcc aacttgcaag tctgaaacga
    14041 ttcaaagatg acgtgaaaga agtcaaaatg ggttatgaat gtggtgcgat gattgaaaaa
    14101 ttcaatgaca tcaaagtaga tgacgtaatt gaaggattta tcatggaaga agtcaaaaat
    14161 gactaaaaac tgataaaagg aggccaatca ttatggctaa ctaccgtgat cgccgagtcg
    14221 gtcaagaaat cttaaaagaa gtcaatgata ttttgcgcaa aaaggtccgt gaccctcgtg
    14281 tggagaacgt aacgatcaca gatgtgaaag ttacggggga cttacagcaa gcaacgatct
    14341 attacagcat tttatctgac ttagcatcag ataagaaaaa agcacaagat ggattgaata
    14401 aagcatcagg attgattcgt cgagagttag ggcacaatct aagtatttat aagacacctg
    14461 aattgttctt tgaacttgat gaatcagttg tctatgggaa ccatatcgat gaattgcttc
    14521 gcgacttgaa caaagactaa tcaaaaaatc ccgcaaatcc tgtgaaatca ggggttgcgg
    14581 gatttttttg ttttcattaa gacgtattac ctaagagttc catgacttta gtctggtagc
    14641 gtgtcagcta accaaaaaag agaaaaagaa aagtaaaaaa ttcgactttc ttttttctat
    14701 agtatataaa tttttgcata tagataaaga tcaagatacc gataacggaa gctagattgt
    14761 aatctatttt agtgacagtc aggtatagcc aagtaactag aagatcagtg gaacctgcac
    14821 cataaaagtt tgagtttgtc ggtccgttga cagttaaaag aaaaatcacg ttgaagttat
    14881 tgatatttcc gattacgccg cttcgatttg gtcacgatcc aaattttgga taatccctgt
    14941 agaaattagc atagtagctg ggataccaat tcacatattg acaaagataa cagagacttt
    15001 tacataaaac ggatcaatca ggaaaggaat tgctcgatcc agccccagtt ttccaacaat
    15061 gtgttaactg gcctagcgtc attcagcaaa ttatgcatga tgaggagcga aatgaactgc
    15121 ggaacagcca ttgtgataac gacaagtttt ttggatcaat gtaaaaaacg tcagcgaaag
    15181 gaaaaatttc gtggtttttt atacctgttc atttggctag atataaaatc caaacaaatg
    15241 aacagaagtg cttattaggt ttcaattggg agtatctgcg ctagaataag agtaataatg
    15301 aaaaggagtg ttatgtatgg attattctag taaagaatac tttgataaaa tgacggcttg
    15361 gtggcgtgct gctaattatc tttctgttgg gcaactttat cttaaagata accccttact
    15421 gagacgtaca ctgaaaccag aagatgtcaa aaaacatcca atcggtcatt ggggaacgat
    15481 tcctggccaa aattttattt atgtccattt gaaccgagtc atcaataaat atgatttgaa
    15541 catgttttat atcgaagggc caggacacgg cggacaagtc atggtatcga atgcttattt
    15601 agatggaagt tacacagaga tctatccaga agtaacggaa gatgagacag gaatgcaaaa
    15661 attattcaag cgattttcat ttcctggagg cattgcttct catgcagcac cagaaacacc
    15721 aggttctatc catgaaggcg gagagcttgg atactctctt tcacatgcag tcggagctgt
    15781 attagacaat cctgaagtaa tctctgctgt cgtgattggt gatggtgaag cagaaaccgg
    15841 tcctttagct ggaagctggt tctcaaatgt ctttatcaat ccagtgatag atggagctgt
    15901 tttaccgatc cttcatttaa atggtgcaaa aatagccaac ccaacgatct tggcacgtaa
    15961 atcagatgga gaattagcaa attactttaa cggacttggc tgggagcctt tctttattga
    16021 aggaaatgat ccagaaaaat taaatcctgt tatggctgaa aaaatggatc aagcaatcga
    16081 gaaaatcaaa tccatccaaa aagaagctcg cctgaagaca gcaacagatg tcgtgatgcc
    16141 aaaatggcca gtattgatcg tacgcacacc aaaaggatgg accggtccaa aagaatggga
    16201 cggggaacca attgaaggaa ccttccgagc acaccaagta ccgattcctg tagatcaaga
    16261 acatatggat catgcagatg ctttactccg ttggctgaaa tcctatgaac cagaaaaatt
    16321 atttgatgca caaggacgta ttttagaaga aatcagagaa attgcgccta ctggtgacca
    16381 aagaatggca aaaaatccta tcaccaatgg tggaatcgat ccaagaccgc ttattatgcc
    16441 agattggaaa aaatatactt tgcaatttga aaaaccaggc tccgttaaag cagaagatat
    16501 gaccgaactt ggtaaattcg ttcgcgagat cattgagaaa aatccagaaa atttccgtat
    16561 atttggacca gatgaaacaa aatcaaaccg attgaatcaa gttttcaaaa cgacaaatcg
    16621 ccaatggatg gaaaaaatcg aaccagaaaa tgatgaatgg ctttcgccat ctggtcgcgt
    16681 catcgacagt caattatcgg aacaccaaga tgaagggttc ttagaagggt acgttctgac
    16741 tggacggcat ggtttttttg caagttacga atcattcttg cgagtggttg actctatgtt
    16801 gactcagcac ttcaaatgga tacggaaatc acatgacctt tcttggcgaa aagattatcc
    16861 ttcgctgaat ctcattgcaa gctcaacagt cttccagcaa gatcacaatg gatatagcca
    16921 ccaagatcca ggaatattga cacatttggc agaaaagaag gcggagttca tccgtgaata
    16981 tctgcctgcg gatgcaaata cattgctcgc agtcatggat aaagcattcc gctcgtcaga
    17041 aaaaatcaat ttgatcatct cttccaaaca tccgcgtgct caattttact cagcagaaga
    17101 agcagccgtc ttagtgaatg aaggcttgaa aatcattgat tgggcttcga cagcaaaaga
    17161 ggaggaacct gaattggtca ttgctgctgc aggaacggaa tctaacttag aagcattagc
    17221 agctgtaact ttgctgctgg aagaattccc aaaactaaaa attcggttca tcaatgtcgt
    17281 agatttattg aaattacgtc atccaagtca agatccgcgt gggttgagcg atgaagaatt
    17341 tgatcaatat tttacaaaag acaaaccgat cttatttgct ttccacggtt atgaaacatt
    17401 ggtccgcacg attttctttg atcgtcataa ccatcattta atgattcatg gctacaaaga
    17461 aaatggagat atcacgacac catttgacat gcgtgtagta aatgaacttg atcgttatca
    17521 tttggcaaaa gacgcagctt taaaaatcaa aggaagtcaa gcagaagatt ttgcggaaaa
    17581 aatggatcag aaattacaag aacatcaaaa ctatattcgt gaaaatggta ttgatctgcc
    17641 agaagtattg gattggaaat ggaaaaatct tgaccaataa aatgataaaa tcgatcccgc
    17701 aaaacagaat tttttacatt gtattatttg tcaaattaag
//
LOCUS       DASJTC010000060        17394 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.060, whole genome shotgun sequence.
ACCESSION   DASJTC010000060 DASJTC010000000
VERSION     DASJTC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.060"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            104..541
                     /locus_tag="U4B19_001838"
     CDS             104..541
                     /locus_tag="U4B19_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077573.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            636..1520
                     /locus_tag="U4B19_001839"
     CDS             636..1520
                     /locus_tag="U4B19_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEN2077574.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            1744..4911
                     /locus_tag="U4B19_001840"
     CDS             1744..4911
                     /locus_tag="U4B19_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077575.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            4931..5476
                     /locus_tag="U4B19_001841"
     CDS             4931..5476
                     /locus_tag="U4B19_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HEN2077576.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            5504..5959
                     /locus_tag="U4B19_001842"
     CDS             5504..5959
                     /locus_tag="U4B19_001842"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HEN2077577.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            5956..6714
                     /locus_tag="U4B19_001843"
     CDS             5956..6714
                     /locus_tag="U4B19_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEN2077578.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(6800..7540)
                     /locus_tag="U4B19_001844"
     CDS             complement(6800..7540)
                     /locus_tag="U4B19_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2077579.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            7601..8854
                     /locus_tag="U4B19_001845"
     CDS             7601..8854
                     /locus_tag="U4B19_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2077580.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            9176..9403
                     /locus_tag="U4B19_001846"
     CDS             9176..9403
                     /locus_tag="U4B19_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEN2077581.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            9526..9852
                     /locus_tag="U4B19_001847"
     CDS             9526..9852
                     /locus_tag="U4B19_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEN2077582.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            9920..10633
                     /locus_tag="U4B19_001848"
     CDS             9920..10633
                     /locus_tag="U4B19_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEN2077583.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            10943..11793
                     /locus_tag="U4B19_001849"
                     /pseudo
     CDS             10943..11793
                     /locus_tag="U4B19_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            11914..13779
                     /locus_tag="U4B19_001850"
     CDS             11914..13779
                     /locus_tag="U4B19_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2077584.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            13827..15236
                     /locus_tag="U4B19_001851"
     CDS             13827..15236
                     /locus_tag="U4B19_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEN2077585.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            15344..16045
                     /locus_tag="U4B19_001852"
     CDS             15344..16045
                     /locus_tag="U4B19_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2077586.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            16204..16683
                     /locus_tag="U4B19_001853"
                     /pseudo
     CDS             16204..16683
                     /locus_tag="U4B19_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
BASE COUNT         5809 a         2918 c         3730 g         4937 t
ORIGIN      
        1 ttttaaaaat tttttttact tagaatccag cgaccctaga tatttttctg tataatctta
       61 aataaagaaa acaatatcaa aaaatgcacg tagaagtaga taaatggagt ggttttgcaa
      121 atttctttta tttctagaag ttattcacgt atacggattt gtttttctca caaataaaaa
      181 ggcaggaagt acaatggatc atttgtatat aaaatatatt catcaatttt ccgttttggt
      241 gttaggcaca ctaattatag cggggattgt cgcatttatt attcaaggcc gtgacatatt
      301 aacagtacat caagagtttt tcagttatgc atttggtatt cctctgatcg ctataaggtc
      361 tgataacaaa aagtggaaga aacgctatta cattctcttg ctatttttgt gtttttatct
      421 gccacctaag atttttactt tattaggtat acaatgccta tcattttctt ttatttccta
      481 tatcattgtt ttaattttcc tcagtttcat agtatggatg ttttatcttg tcaaaaaata
      541 aatgaatgtt tcatgaacag tacttcatca cagcctaatt tttttcttta tgctatcgtt
      601 atattaaaga aatcaatcag aagggaaaga aagtgatgaa aaaagtttta ttagtggtaa
      661 accccagttc aggcggtgaa caagcaaagg aatttgagca attagcgata gcaaaattgg
      721 aatcggtgtt tgatgaagtg gtcgtcttgc atacaaaaaa agcaggggat gcaaaaaact
      781 ttactcgcga agcggctacg gaagggtatc atagtgtgtt tgtcatgggc ggagatggaa
      841 cagtcaacga aggaatcagt ggaatcgctg aacaagaaca tcggccaaat ttcggatttt
      901 tcccattggg tacggtgaat gacttggctc gagcattagg gataccgcta gaacctgagg
      961 aagcgatcaa tcatttttcg attgaatcag tcaagccatt ggatattgga aaaataaatg
     1021 acgactattt tatgaatgtc gtcgcaattg gctctatccc tgaatcaatc aatgatgtag
     1081 atgcggagaa gaagacgaaa tttggaaaac tagcttattt catgtcagga atcaaacagt
     1141 tagcttccac acaatcctac tcgtttcacg tagaagttga tggaaagaag gaagaaattg
     1201 agagcagtac cttgctgata ggactaacta actcagtcgg cggttttgaa acattacttc
     1261 caaatgcaaa agtggatgat ggtaagctcc atttggttta tttaaaagat tcttctttga
     1321 tcgatacgct taaaacattg ccagacctat taaaaggagt agatgaatct acgaaaaatc
     1381 tagcctacct tacttgtgag caattagttg tgtcattagc tgatgaggaa gaattgacga
     1441 cgaatgttga tggagatgaa ggggataaat tacctgtgac aatcaaagta ttgccctcac
     1501 atttacaggt ttattgttaa aatggaggtt gtgaccgagg agttggcggg agaacaccgc
     1561 ttgttcggga taagggactg tgacaagact tttgtcacag cctttttttt gtacaaaaaa
     1621 gaaatttaca cctaatagaa aaatttttcc ataaaaatac atattttaaa tatgaaagta
     1681 caattggaaa tagattaaaa cgataaaata aaattgtcaa aacgtcagag aggatggtgg
     1741 agaatgaatc aatgtgtaaa aaagctgatt gcgtcgattt tgtttgtctt ccctctcttg
     1801 atggtgacat ccgtttttgc cgatgtagag aaaacaaacg aatcaacgca atcagagaat
     1861 caaagttttg acaagactac tttttcagaa gaatcgatta cttctgaaac tacagaatct
     1921 caaagtgagg cttctactaa taaaaggcaa gaagaaaata agacaagtga gacctcagaa
     1981 gtagctgaac aaacgtctga tagcagtgaa gcggcactgg caagagctgt ttcagcggat
     2041 gggtacagcg aagctgccac aacagaagaa ctggcgcagc ttctcgcaga cgaatcagta
     2101 actaagatcc gactgattca gcctttgact ttagacaggg aattagagat caaacgagat
     2161 atcgttattg attttggtgg attcgctcac aatttcggca cacaccatat ttatatcaat
     2221 gagatacctc agtcgatcga gttccagaat tttaaaggaa cagctgcaca tccaggtgga
     2281 ttgacaccaa atatcgatgg aaatgccatt atcattgctt atatgaccga tttttgggga
     2341 agcagatatc attttacggg agaaataaaa tttaccggga cacttgattt gtatgatggg
     2401 tccaaattag gactgattta cgctcctcgt gcaactgtca cattagatgg tgtttccggt
     2461 gtgttggatg tccaaccagt gaaggaagga ctgaatgccg ctccgggtaa agcctatttt
     2521 tgtagaagct atcaattgaa tgtgatcaat ggtagtcaac tttatggacc gtatctaggg
     2581 aaattctacg gttttctaga ttcaggagat gagactggta gtggaaaaac agcgccagga
     2641 atccatatct taagcggttc aaaggtctct cttgattatg atcgggcaga tggcgttagc
     2701 gatgaaggag aggcagtcga tacgctacct ggtaatgtca tgtttgaagt cagtggttcg
     2761 ggaagcgaat tttcggtaaa tacaaagatc aatattacca atgacaacaa tcgcggaatc
     2821 atccaaatga gaggttcagg atcgaaagtc actgtatcga atggtgggaa aatcaccatc
     2881 aatacagcaa ctacgggcgg atttcgcttg caaggagatt ctagtcaaat ccatgtatca
     2941 agtggtggac aaatctcggt caaaatggct ggagatgacg atcaacccgg gaacaatggg
     3001 atgcgttttg taggaaaagg cctttctttg atcgtagaag gagccggatc agttatcgac
     3061 atcgataagc agagtgggcg ttccagcgca gttcgttttg agaatggtac gcaaaagctg
     3121 acagtaacca atggaggctc attgagggta agaaatacag gtgatggcaa atcttacgtg
     3181 aacagactca atcaaggaaa tcaagcgatc caattttatg acgcttctgg ttttctagaa
     3241 agtccaggtt cagctgattt tacagtcact ggagaaaaat caaatatcga tatacgcgca
     3301 gattccggag caactgtaga tacaactgga gatattgatt taaattttac ggcaggtgaa
     3361 aaaacttatt tagtcatggt cgggaaaaca ggggaagata agaccggcat catcagtggg
     3421 gatgtgttga atgtggactt agaatctcct acctattttg atttccaaaa caaacggatt
     3481 ggaagcgaga ccaatggcat aggcggctgg gtattccaag gaaatggaga ttcttcttta
     3541 aagatcgccc attcagaaat cgctttatgg cgaaaaacag gcgcaacatt cgataacttt
     3601 gaaaaagacc cggattattt ttctgatatg acagacttat cgatcaatgg gaccgactta
     3661 ggcaatttcg tatcaagccc tagtgcaggt ttaacaaatg aatttgctca gacaggtgcc
     3721 ggaatgaaaa attataatcg aatcagtgcc aataatcagc tcccgatgat tgaatcctta
     3781 agagtgccaa cgaatgcaga taaaaaaatc tatgggcatg tcgttgttcc cgaaggtgtc
     3841 gaggcaaagc caagagatgc ttggacaggc gaagtagaag tgactttacg tattacttac
     3901 gcagatacga cgaaatcatc tgtagaaatt actgctctca cgcaaggaca aacagatgaa
     3961 aatgaagggg caggatataa cccatgggga gaaggcgaac aaggcggctt gttcgaagtt
     4021 caagtaccca atgatgagag attaaaaacg gaggatcaag tgcaagttgt tgctgctaaa
     4081 aaagtaagag gtggccaaga agtgacagaa ctggactctg ccaaaacgac agtcgatgtg
     4141 attcctcctg aacctgctaa tttaagtacc acattgatca atgcagcttc tcgcacgatc
     4201 acaggtacag gtgaagcagg agccttggtt tcattgaaaa aaaatcaagc tgttttgtca
     4261 gaaacaacta ttgatgatac aggggaattt gttctttcta tcccagaaaa tcaacttttt
     4321 gcaggagatg tattagaaat tgtattaaac gaccaagcag gagaagctac tgctaagggt
     4381 gtgcagaaca agccaagtac caacgatgaa attgggaatc acaatccaag tgatacgcca
     4441 gttgtttatc atgatgcccc gccttttgca cctgctacga agatcgtagt agaaggtggt
     4501 ctctcatttc ttgccccttc caaactcgat tttggtcaga tcaaagcaac tggtttgaca
     4561 caagaagcat ttggccaaat ggatatacag gaaaaattat ggatttatga tcagcgagaa
     4621 acgaaaaatc cgtggatctt aacgataaaa ttatctcagc ctttcacaaa tgatgcagga
     4681 ttttctatgc aggatgtcct atatttcaag aaaggagagg agtatcaatt agtgaatgaa
     4741 gaagaactgg aaattgtcag aggacaaaat tcaacgaatg tgcaagcaga ttattcagaa
     4801 tatttaaata acggtcgggc gtttaaactt gtcttaacca aagaaaatca gatagctggt
     4861 aattactcag cagagataac atggacatta gcagatgcgc cgacagaata aagatcaagg
     4921 aggatgattg atgaatcgaa tatggaataa aagtcatcga aacaactgct ctttacttat
     4981 gatccttctc cttggattca tcctttggca gacacctgtc catgcctccg aattcaattt
     5041 tgcggtagat tttgaattac cggctaatca gattgataag aacaaaacat atataaatgt
     5101 aaggatggag cctgggaaaa cacaggaaat cccctacacg gtctttaacc gaacagataa
     5161 atcagtaaca gttgagacat tgatccactc cgctacgacg aattcaaacg gtgtgatcga
     5221 atatggagaa tcaaaaagta aatttgatga aacattaagt tatgatatga aaaacttagc
     5281 aaaaacagtg ggagaaataa ctgttcctcc tcaaagtgcg gtcactgaga cactttcact
     5341 gaaagcgcca gaaaaagagc tggctggtgt ggtcgcagga gggatcacgt ttcaagaaaa
     5401 aaaagagaac gtttctaaca aaagtacagt tggtgtcgtc aatcagtttt ctcaagtgaa
     5461 agcaatcttg atttgaaata gcgaaaaaga gtcttcccca gatttgaaat taaaaaaagc
     5521 ttatgcggat caaagcgatg gaagaaatgt gattaaggcg aatcttcaaa atccaaatgc
     5581 ttcttatatg ttcggcttga ctacatcggt tgaaatctac aaaaaagaaa caaaagagcc
     5641 ttacctttca ataaaaaaag aaaaaataga tgtagcaccc aatacaaatt ttgatttact
     5701 tgtcccatta gaaggaaaaa aattagctcc gggacattat caagcggttg tgaagggtgc
     5761 ttggaaagaa aaacgctgga atctccatac cgattttgaa ataactgatg atgcagcgga
     5821 ccaattgaat gaaaaggata ttgatttaaa aaatcagcca gaaacatttg actggcgatg
     5881 gctgatactt gttggagtca ttcttattgg cttatttgct ggattattta ttttaaaaga
     5941 aagaaggaaa aagaaatgaa aaaaacaatt gcattaagta ccgttctagc tggaagtcta
     6001 ttattatgta cacaaagtgt ttttgctgag aataagagct cagccactac agaaggaacg
     6061 atttctgtga ccaaaccaga tgctgcaaat cctgaagacg ttgttccagt tcctcctgtt
     6121 gatccaaatg atcctgatac tccttttaca cctgacgaac caaatcaggg aacatccgga
     6181 ttactgacga ttgaccaagc tccagatctt gattttggaa caatcaagtt aggtacagca
     6241 aaaacagtat atgctgcatt acaaagcgga acggacagta ctggagcaca aacacaagta
     6301 cagaatttcg tacaagtaac ggataaaagc ggaaattatg ccggttggaa attatcagtc
     6361 aaacgaacag aatttattgg tacaacatct gatacgaata aactaactgg ttcaaaggtg
     6421 aaattcaaaa acgccgtgat tcgaaatagc gcaactaata gcagtggaat gccatctacc
     6481 gtttctggaa ctggcgctga aggaatcgaa atcccagcag atcaggcagt agatctagta
     6541 actgcaggag caaatgaagg gattggcaca tggatttatt cattaggaga gaatgtggaa
     6601 caagggagac aatcagtgga attatttgtt cctgtatcca attatgctgt agacaactac
     6661 gtttcaacat tcacatggtc gttaacagat gctcctacag aagaggaagc gtaaaatcat
     6721 atcaacgatt tcagggagtg gagccgattc aaggcccact ccctgaaatc tgctatgctt
     6781 acgtctcagc tcttattcat caactcgaag cagttttttg gaagtcatgg aaacgttcct
     6841 ttgtcttttc aaagatcttt cgaccaacga gtaaggagtc accgttgaaa aattctaaca
     6901 cataatgagc agaatcaatt ttctgcaatt gcgataagtt gatacagtaa gattttaaaa
     6961 gtaaaaagta agcatacttt tctaactctg tcttcaattg ttttaaagaa gtattcacta
     7021 atttatcttc tgtagttgtt ttgaccataa cttggttttt caaacctttg atactttcaa
     7081 tatataaaat ttcccgagga tacaggacga gacttccacc tttttgatca ataaagattt
     7141 tatcgacatt tacgatctgc tgttctttcc aattgatctg ttctagcact tgacgaattt
     7201 gttcagataa attcctgtct tttctgatat atccaatcgg aaggattttc cgattgatga
     7261 tctctactgc aaaattcgta taagcggtga aaaaaacaat attactatgt gcattttttt
     7321 tacgtatttg ttcggcgaca tcaatacctg tgagtgattg cttcaagtcg atatcgataa
     7381 agaaaacatc gtgattatca atagtgccca actggatcaa ctgattgatt tgagagatag
     7441 aagtgagctg cacgatattc tcgatatgat gattgaccaa ttctctgatg atccattcgc
     7501 tgtgaaactg gttatcttct atgataaaat actttttcat atgcaggctc ctattaaaaa
     7561 aatatgtgta cattttgaaa caaaacaatg gaggaataag atggtcgcaa cagtgacact
     7621 ttatttgacc tttactttga ttttagattt aggtatctat ttattctttt cgaaacaact
     7681 gctagtaaaa aacagctttg gttgtatagc tttgatggtg gtgacacttt tacttcctat
     7741 ccctaaagtg agctttcctt acctagttgg cttattattg atgtttcttt tttattttac
     7801 tacgaaaaac ctgtttatca gctgtttgat cagtaatctt ttttttgcta tcactggact
     7861 cagctttttt ctggtttttg atctgatgga ttatttagga aagagacatc ttggaacagc
     7921 aagttttttt gcactactta tcgcatttat tttctatcta ttagtaaaaa gtatagacaa
     7981 acagacgaga tttctcaact tgctttttac ttatccaact gaacactatt ttggaggtat
     8041 ttgtatttta ttaagctggg gcgtgattta cttggtattg ggaagtgttc acttccgctc
     8101 atctaattat ctgcttctat ctcttttgtc gatagtcatc agtttttttt ctttgttttt
     8161 ttccattatg ttgatcagtt tccaagtaaa agaaagacaa catcaggaat cgcttcaact
     8221 gtatcaaata aatgaagaat attaccatca tctagaggaa ttcaaacatg attataaaag
     8281 tctactgttt tcattaaaaa ttactcttca ggaaaataaa aaagaaacgt tggaattttt
     8341 agaaatatta gaagatcatt ctgcagctat ttttgaaaaa ccgcaaatac aacagctaac
     8401 aaatattgta tccccagctg tcaggggtgt ctttttaaaa tttctagaaa aagcagaatc
     8461 ggagaaaatc ccgatccaag tagttattcc tgtcgaagtg aaaacaatcc cgattgatct
     8521 ggttgttttt attcgctgtt tatctatttt tatttctaac gcattcgatc atcgtgtagc
     8581 cgatcagagt ccgattacga tcaatctgct ggaagaaaaa gagggatcac gttttagtat
     8641 agcgaacaag gcaaatccaa cgaatttgac gctatcggag atggtcaaaa gaggaaaaac
     8701 ctctaaaaaa aatggcgggt taggtctata cagtgcgaaa aaaatgttgg atgtttacga
     8761 aaatgcagaa ctaaaaatcg aattttcaaa acagaatcac tatttttttg tagaaatgta
     8821 tctggcatct agaaagagca aatcagccta ctgacttact gtcaaaggat aagtttgagg
     8881 aaagtctaca cggagataga gacaacaaca cagcatattc aaaagaaaca gaagcgtgat
     8941 gaccgttgtc acccttctgt ttcttttgct atgtagttaa acaaagtagt tgacacttcc
     9001 gctacttagt attagtacga aagtatactt catgatacta agtagataag aagtgaaaag
     9061 cggaacccac gatcgtcaaa atgctaactg ctttgcttac accagataaa gggaagagca
     9121 atatcgctta ttttaggcgt ggagaagcaa gcaaatccct acgatcacaa aaaaaatgct
     9181 gacccagcag ttaagagagt tagaacaaga tgaaagtatc catcgaaaag cctataacca
     9241 agtcccgcct aaagtagagt actcgctcac cgaagaagga aaatcattaa gagagattct
     9301 agtagcgttg tctgtttggg aagaagaacg tatcgaaaaa caaaaagcct caagggaaga
     9361 agacacgcta ctgcatacac atggtggcta cctaaattat tgattttcta tgacatttat
     9421 tttaaataaa agaaaacagg agttttttta aaagcatcat ctatttttta tgtaaaaaaa
     9481 tggtatgatt aatcaagtaa gaagtgattg gaagaaggca agcaaatgaa tagtatggaa
     9541 aaagtcaacc agtttagaga tgaacgtaat tggcgacaat ttcataacga aaaagatttg
     9601 gctatctcta tttctttgga agcatctgaa ttgttagaac tttttcaatg gaaaacgccg
     9661 gaagaagtaa aagaaacaca attagaacga atcaaagaag aactggcaga tgtattgatc
     9721 tattcataca tgatggcaga caaccttcac ctggatctag atgagatcat tgaagaaaaa
     9781 ttgatcaaaa ataatttgaa atatcctgta gaaaaaagcg caggtgtacg aaaaaaatac
     9841 acagagttgt agaaatgtgt cattcggctg ttggaaagag caaaagaaaa acagtaaaat
     9901 gagtctggag ggagagaaaa tgaaaaaaaa aggaaaagca aaaagatggc tagtcaatat
     9961 cctcttgttc ctcttgttat tagtaggact agctttgatt ttcaatgaac agatcaagga
    10021 ttattttgtc agagagacag gagataaata tgctatagcg aatgtaacaa aagaagatct
    10081 gaaaaagaat aacgataaag atgtcagttt tgactttgat gcggtagaac caatgacgac
    10141 agaaggggtc atgaggtcgc aaatgagagg aacagatcta ccagtgattg ctagtatcgc
    10201 agtcccttca gtttcaatca atttaccggt tttcaaaggc ttggataata cgtcactact
    10261 ttacggagca ggaacattat ctccagatca agaaatggga aaaggaaatt atgccttagc
    10321 aagccatcgt gcaacgaacc ctgaattgct gtttacaccg ctagaaaatt tagagatggg
    10381 tgccaagatc tatttgacag atttagaaaa tgtgtataca tacaaaacat tttttaaaga
    10441 aaaagttgct cctacagata cccaattact gaatgaagtc gagggaaaag aaattgtcac
    10501 actaattact tgtggcgaca tggacgccgt cacgcgacta gttgtacaag gagaactgga
    10561 aagtgttaca tcgataaaag atgcgacaga tgatatgaga tctgctttca atctagagac
    10621 aaaaacattt taggatactg ggacataagt cgttacagat taaaaacagc caaaggatat
    10681 gaatcgataa ggaaaaacca atttgctgaa aaaaagagaa acgcaaagag gtgcatctct
    10741 gatttggttg ttttctagat agacttgtgt tccagttttt ttatttttta tatgaaaagg
    10801 cttgcataaa taaaaattcg atggtatgat gattttgaat aagaaatgga ttgttactgt
    10861 acggcaggca aaaccagaat tcagctaaga aacagtaggc gaattctagg tttttttatt
    10921 tttcttgaga gggcgaagca aaatgaaaat tgataaaatt ttgaataaca atgtcgtcat
    10981 ttcgaaaaat ggattcgggg aagaagtcgt ttgtatggga aaaggactag cttttcaaaa
    11041 aaagattggg gacgagattt cgccagaagc ggtccaaaaa gaatttgttt aagagattct
    11101 tttgctaaaa accaattcca gcagctgatg gctgatattc cggcagagga gatcgaatgg
    11161 gtcaaacaag tagtcgaact ggctgaagat aaactaaaag ttgaattttc gcctaatatt
    11221 tatctgacat tgaccgatca tcttcattat gcgatcacac gagcaaaaga aaatatccaa
    11281 ctgcctaatc cattattatt tgaaacaaaa aaattttatc cgaaagaata tcaagcagcg
    11341 aaagaggctc ttttgttcgt gaaacaaaag acgggtgtgg aactttctga ggatgaagcc
    11401 ggttttattg cctttcattt tgtgaatagc caacaaggaa acgaagacat gcaagtgacg
    11461 atgaccgcta ccacaatggt aagagatata ctgagtatca tcagtaaatt cttcggcatc
    11521 gtgttcgatg aagaatcttt gaattatcag cgtatcatta ctcatctgca gttttttgcc
    11581 cagcgttatc tcaaagggga aggatctgac gagcaagatg aatttctcta cgcattagtt
    11641 caaggaaagt atccaaaagc gtttcattgt gtcgaacgga tcaatgagta tcttcgacaa
    11701 acgcaaaaac aggaaatggg gatcgcagag cagatttatt taacgattca tattcaaaga
    11761 gtagtaagtg agaaaaaaag catccaatca taaggattgt tactgtatgg caggcaaaac
    11821 ctggattgaa aaaataggtt cacgaatgtt tgtgactgtt tttttcaatc tgggttttta
    11881 ttttttaaat aaaaaagaaa gaaggaaaaa acaatggata atcaagcagt tggtcgtcgt
    11941 gtatgggaag cggtaggagg acaaaagaat gtcaaaagtt tagtacattg tgctacgcgg
    12001 ttgcgattca gattaaaaga tgaatcttta gcagatacgc aaaagttaaa agaagacccg
    12061 gacgtgatcc aagtcgtaca aagtggcggt cagtatcaag tagtgattgg cagtaatgta
    12121 gcagatgttt atcaatcgat cgtggatgag gaaggtcttg cagtttcgga ggatggaaaa
    12181 gaagagtcca aaaatatttt gaatcgtctg atcgatatta tctctagtat atttacacct
    12241 tttctagggg cgatggcagc cgccggggta ttgaaaggat tcttatcatt ggcaacggtg
    12301 atgggctggc tgacagctga tagcggtact tatcaaatat tgtttgctgc agcagatggt
    12361 gtctttacct tcttacctgt catgcttgct tttacagcag ctaaaaagtt caaagccaac
    12421 cagtttttag cagtagcaat tgctatggca cttgtgtatc cagctatcac agcagttgct
    12481 ggtgcgggag aagctatcag tttctttggg atcccagtga ttctttcacc atctggttat
    12541 acttcttctg tcataccaat cattctagcc gtatgggttc aaagtaaatt agagccattc
    12601 gtaaaaaaag tgattccgca atttctgcaa atgattttag ttccgcttgt cgttttagta
    12661 gtgatggttc cattaacatt tcttgcttta ggaccaatcg ggacggttgc cggtaatgct
    12721 ttaggcggtc tcttcaactc gatttatgga tttagtccaa tcgtagcagg tctaatcatg
    12781 ggaagtttat ggcaagtatt cgtgatgttc ggcatgcatt ggggattcgt accgatcatg
    12841 tttttgaata tcgaacaata tgggtttgat gtattgatgc caatgcttct tccagccatc
    12901 ttggcacaag gtggggctgc tttagctgta gcattgcgaa caaaagatac gaaacttcga
    12961 gcattaggga tttcttctac agtgacttcg ttatttggaa tcactgaacc aactgtgtat
    13021 ggtgtgacat tacctttgaa gaaaccattc attgccgctt gtatttctgg aggtattggt
    13081 ggagctatta tcggattttc cggagtaaaa gctttctcca gcagtttggt cagcttattg
    13141 acgattccga ctttcattaa tacggtggat ggtgtagagt caaatgtgac agtagcagta
    13201 atagctacag gaattgcttt tgttcttgca ttcgtaggta cattgatttt aggatttgac
    13261 gaacaaacac aagacaatca attagaaaac aaacatgcga atgcaggaga accgattact
    13321 tcagcacggc ataccttaaa aagtccttta acgggtaaag tactgccatt gagtgaagta
    13381 ccagaccaag ttttttcttc tggcgtgatg ggaaaaggta tcgctattga tccagaagta
    13441 ggagaactag ttgcacctgc agacggagag atcacgacta tttttccaac tggacatgct
    13501 gtagggatca caacgactga cggagcggag attttgattc atatcgggat ggatacagta
    13561 gaattaaatg ggaatggctt tgagatctta gtcaaacaag gggatctcgt caaagcagga
    13621 gatttattga ttcgttttga catcgaagca attagagcag ctggctatag cgtaatcact
    13681 ccagttgtca ttacgaatac tgatgcattt gccgatatac ttgaattaga ccaaaaagag
    13741 attattgcaa acgaagatgt tctggcaatt gtaaaataag gaggaaatca ggatgcaatc
    13801 acgtttttca gaaaaatttt tatggggtgg agcaactgct gccaaatcaa ttagaagggg
    13861 cttatgatcg agacggaaaa ggactatccg ttgcagatgc tatgcctgga ggtaagcagc
    13921 gctttgcgat tatcggcagt gaagaatttg actggacgat tgatcaagaa aaatatattt
    13981 acccgaatca tcgaggaatc gatcattacg atcgtttcaa agaagatctt gccttgtttg
    14041 cggaaatggg attcaaatgt tatcgttttt cgattgcctg gacacgaatt tttccaaatg
    14101 gagacgaagg tactcctaac gaagcgggat tagaatttta tgatcagctg attgatgagt
    14161 gcttaaaata tgatattgaa cctgtgatca cgatttctca ttacgaaatg cctcttcatt
    14221 tggcaaaaga atacggtggc tggaaaaatc gaaaactgat cgacttctat gaacgatttg
    14281 ctcagacagt attagagaga tacagcagta aagtcaaata ttggatgact ttcaatgaaa
    14341 tcaattcggc ctttcacttt ccagctttaa gtcaaggatt agtaaaaagc aatggagcag
    14401 gagagtacca aaatattttc caagcatggc ataatcaatt cgttgcaagt agcaaagccg
    14461 taaaaatcgg ccacgaattg cgttcggata tccaaatcgg ctgcatgatt atctatgcaa
    14521 caacttacag catcgatgcc aatccagtca atcaggcagc aacaatgatc caaaaccaag
    14581 aattcaattt tttctgcacg gatgtccaag tacgtggaga ataccctgca tacacagcgc
    14641 gtacccataa aaaatatgga gtcgatccgg aaaaattaga gcaaacggaa aaagacttcc
    14701 agttgctaaa agaatatcca gtggactaca tcggatttag ttattatatg tctacggcaa
    14761 tcaatgagac tgatccacaa gcagcaactt cagaagggaa cttgctggga ggagtaaaaa
    14821 atccgtttct tgaggcaagt gaatggggct ggcagatcga tccagaaggg ctgagaatcg
    14881 cattgaatga attatacaat cgttaccaga aaccattatt cattgttgaa aacggtcttg
    14941 gagccattga tcatatggaa gaggatggca cgattattga tgattatcgg atcgattatt
    15001 tacgtcgtca tatcgaagcg atggctaatg cagtagcaga cggtgtagat ttgatgggct
    15061 atacgccttg gggatgtatc gatcttgtca gtgcatcaac aggagaaatg agtaaacgtt
    15121 acggctttat ctatgtggat ctagacgatg aaggaaatgg gacactgaat cgatcaaaga
    15181 aaaaatcttt tgattggtat aaagaagtaa ttgcctcaaa tggcgtaaac ttgtaattaa
    15241 agcagctgaa tgaaaaagaa cacacttctc acatgtgatt tgtgttcttt ttttattttt
    15301 tatgtgctat tcttttagta atagtaaagc aaagggtgac gttatgaaaa ggacacgtgt
    15361 actatacttg gaagtcgcag ataaaattaa agaagatatt ttctcaggaa aatatccagt
    15421 cggttcgatg ttgccgacag aaagtgaatt agaagaactg tttcaagtca gcaaaatcac
    15481 tgtccgaaag gcaatcgaac tgctggcatc agatgaatat gtcgcaaaga aaagtggtcg
    15541 tgggacgacg gttctaagca atcgtcctta caatcgatta tccaaagcgg caagctttac
    15601 gcaaatcttg gagaactccc gatatgaagt ccggaaagaa acgctggatt tgcaattggt
    15661 ccatataaag aaggaagatc ctttgttttc ctttttcggt gagcaagctg tttgttttaa
    15721 gcgcttatat tatttgaaca atcagcctta tatttatttt gttcattatc ttccacaaga
    15781 aatgatgcaa ttcagcagag aagaatttgg caaagaatct ttgtatcgtt tgctgacgaa
    15841 aaaaggatat actattgact cattttctga tgatttctcg gcagtatttt taacagaaga
    15901 agaacaagga attctgaaaa cagacgcaca gatcggcatc aaacggatac ggaaatcgtc
    15961 tacgcaaatt ggggaaatga ttgaatattc ggaggcggtc taccatacgg agctgcatcc
    16021 ttaccatatt gattatgaaa cataaataaa aaaagaggtt gtgaaaaagc tgccctgcat
    16081 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttaggctta
    16141 ttaccgcaga tacacggctg tttctggatt accatttatt ctggataagg gggtgtggca
    16201 cgattttcag aaggaatcta tgaaccaatc actgtattga ttttagcttc gatggtaacc
    16261 ccattcggct tgacgatcgc ttatttctta gggaaaatca ttcgtaaaaa tatactcaat
    16321 cgtcaagaaa tcgatacatt gaagaccgct tttcctatgg ggatctgtca gatcacagag
    16381 ggatgtttcc ctatcgtatt gaacgacttg gcacgcaatg tcatagcaac gggagtcggt
    16441 gggacagtag gtggcggatt aagcatgttt tggggcgcag atagccgtat tccggcatcg
    16501 ggaatgtttg cagtcgctac gatgaccaga ccatgggcat tcattggtgc attgttagct
    16561 ggttcttttg tcacaggaat aaccatttta ttattgaaaa aaccagtaga tccgaatgca
    16621 gaaatcatcc aagaagaaaa agaggaagaa gacatatctt gggatgattt gacaattagt
    16681 taggagaggc agaatggatt acaagaaaaa agtacaatag atgtaccttg agacaggaat
    16741 ggtatgaaag aatcagagtt agaaagaatc gaatatacag attttggttt aggagcgatc
    16801 gaaactgtcc agagcatcgg caccctcctt gaaatgacga tgaaggaaaa atgagaacct
    16861 ttcgttgtcg aaagggtctg gtatacctta tgtcgaagga aaggaaacaa aaaaactttc
    16921 ctgtactatt ccaaaaagga aagaaagtta ttatacagta aaaaaagaaa ttattcttca
    16981 tgctagagat cagtacacgt tcgagcccaa tatcaaacac tagttccagg caggcgcaac
    17041 aggtgcggtt atcactgaat tttcttccag cagtgacgat tcaagtgata tttttacaaa
    17101 tccagctatt tatcgttaac aaaaagattt ttaggagaag ctgatgaaca aaaatgatca
    17161 aattttgatc gtttagttcg tcggcttttt tatttagaga aacatcattt atcagtgaaa
    17221 cataacacga gaacaaaatg attttttcgt taataaaaga aacaagtatt tgttgtttaa
    17281 aataatacac gttattggca tataggacga aatcccagta tcttctataa gtaagtcatt
    17341 tcatatcgat gaaaacacgt tatttatttt tgttttatct ataaaatggc tgaa
//
LOCUS       DASJTC010000061        17257 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.061, whole genome shotgun sequence.
ACCESSION   DASJTC010000061 DASJTC010000000
VERSION     DASJTC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.061"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(20..565)
                     /locus_tag="U4B19_001854"
     CDS             complement(20..565)
                     /locus_tag="U4B19_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2077587.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(577..1269)
                     /locus_tag="U4B19_001855"
     CDS             complement(577..1269)
                     /locus_tag="U4B19_001855"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEN2077588.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1288..1572)
                     /locus_tag="U4B19_001856"
     CDS             complement(1288..1572)
                     /locus_tag="U4B19_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077589.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1585..2148)
                     /locus_tag="U4B19_001857"
     CDS             complement(1585..2148)
                     /locus_tag="U4B19_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEN2077590.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2376..3038
                     /locus_tag="U4B19_001858"
     CDS             2376..3038
                     /locus_tag="U4B19_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEN2077591.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3162..4358
                     /locus_tag="U4B19_001859"
     CDS             3162..4358
                     /locus_tag="U4B19_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEN2077592.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4459..5835)
                     /locus_tag="U4B19_001860"
     CDS             complement(4459..5835)
                     /locus_tag="U4B19_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2077593.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5872..6739)
                     /locus_tag="U4B19_001861"
                     /pseudo
     CDS             complement(5872..6739)
                     /locus_tag="U4B19_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(6828..7853)
                     /locus_tag="U4B19_001862"
     CDS             complement(6828..7853)
                     /locus_tag="U4B19_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEN2077594.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7871..8545)
                     /locus_tag="U4B19_001863"
     CDS             complement(7871..8545)
                     /locus_tag="U4B19_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2077595.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8729..9622)
                     /locus_tag="U4B19_001864"
     CDS             complement(8729..9622)
                     /locus_tag="U4B19_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEN2077596.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9696..11393)
                     /locus_tag="U4B19_001865"
     CDS             complement(9696..11393)
                     /locus_tag="U4B19_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEN2077597.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11386..12699)
                     /locus_tag="U4B19_001866"
     CDS             complement(11386..12699)
                     /locus_tag="U4B19_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN2077598.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12699..16736)
                     /locus_tag="U4B19_001867"
     CDS             complement(12699..16736)
                     /locus_tag="U4B19_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEN2077599.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16749..17207)
                     /locus_tag="U4B19_001868"
     CDS             complement(16749..17207)
                     /locus_tag="U4B19_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2077600.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4832 a         3887 c         3009 g         5529 t
ORIGIN      
        1 atcaaatcac tttgtttttt tataaaattt ctgcgcctag tgtggactca aaatgtctca
       61 atgcccattc atgtccaacg ggatcgaaac ttgccacgga atctttgaaa atatggagtt
      121 tgtatcctag attatacgca tcgactgctg tatgcaacac acagatatct gtacatacgc
      181 cggtcaaata aatatctgtg atctggcgtt ctcgcaaacg gatgtctaga tctgttccac
      241 taaaagcgga ataatgccgt ttgtctatcc aataaacatt ttcctcttgc tcatagtctt
      301 ggtataaact ttttaaagag ccgtacaatt cccgtccacc cgttcctatc acattatgcg
      361 gaggaaataa gcggttctcc ggatgaaagg aatcttttgg atcatgcgca tcaatggcaa
      421 aaacgacaaa atccttttga tcaataaact tctttgtatg agcagtcagc tttttttcaa
      481 ttacttgtcc tgctgtccca gtagtcagct ttccatcctc tgcaacaaaa tcatatgtgt
      541 agtcaatcga gatcaatgcg ttcatcttag tcctccttat acgagatgtt tgacaaattc
      601 gatcaccatt tctgctgatc gtttgcctgc gtcttcgata aattcgtcaa agctttgtgt
      661 cgccgagtga tctgctgtgt cgctcattgc acgtacgatc aaaaatggga tattgaactg
      721 acgagctgtt tgtccaacag cggctccttc catttcgcaa gctaatgctt cagggaaatt
      781 cgttaagatt tctttgattt tatctggcga gtcgacaaat gtatcacctg taacgatcaa
      841 tccttctttt gcgttcaaat tcgttgcttt tgctgcttta accatttcac tgcgcaaata
      901 tgtgcttgct tcataatata gaggcattcc tggtaattgt cctgggttgt agccaaatcc
      961 tgttgcatct gcatcaaaat aagctacttt gtctgatata acgatgtcac ctacttgtaa
     1021 gccttcaccg attccgccag ctgaacctgt attgatcacc atgttcacgc cgtattgttg
     1081 gatcaacaga cttgtggtaa tagatgctaa gacttttcct ataccggaac gtacgacgat
     1141 cacttcgtgg ttacctaatg aacctgaaat aaataaagcg cctgcgcgtt cccatgataa
     1201 tggttcgctt aggttctctc tcaaaatttt tacttcttct tccattgcgc cgataatacc
     1261 gattttcatc tttttaattc cttctttcta tataaatgcg attgctaaaa aaaccacaca
     1321 taacattagg ataacaataa taagagattt catcaaaaaa gagttccatt tggtcgtctt
     1381 ttcccgttcg ctgatccttg ttttcgtgat tggtttattc tttttgcttt ctttacgata
     1441 aaagctggca atcttttttt cttcttgctg ataagctgtt tctgcttttc gctgtttttt
     1501 taatgactcg cttgcttgtg cttgttgtcg cttgcgaagt tcgcttcgcg taataagcgg
     1561 tcctttggcc ataagccgat ccctctactt tcctttggtc aatagttccc agtattgaat
     1621 cgcataaatc gtctttgcat cacaaatcaa ctgatctttc atcgcttgtt gcgcctcttc
     1681 aagagttaga tgatacaact ccagtacttc gtcttcatct tgtgctaaag gattctcgac
     1741 cttctctacc ccttgtgcat gataaatatg aaggacttca tttgcaaaac ctggtgaaag
     1801 atacatcgaa gtaagatggc tcagcgactt cgcacgatag ccagtttctt cttctaattc
     1861 tctagctgct gtcatttcgg gattttgtcc ttctcctgga tcgatttttc cagctggaat
     1921 ctccaaaatc actttttcta aaggttttcg aaattgtttg acaagaagca gacgatcttg
     1981 ttcgtcaaaa gcaatgatcc cgacaccgcc aggatgaaat accagttctc tcttcgaagt
     2041 tcctccatct ggcaaacgaa cttcgtctac agcaacatca ataatttttc cgctatagat
     2101 ttctttgcga ctgattgttt tttcttcaaa ttgctcattg ttcagcatat cttctcctgc
     2161 tttctttgta atcataactg cttttttttc atttttcaat ttttctcaaa aactccttat
     2221 tagtttacca tagataaacc ttagattttc tctaaatata gaaaatcctt ctttagtccg
     2281 atactctttc tagtagtccg tatgctaaaa taagtatgta atcgaataac gaaaagaatt
     2341 caatactcaa cgataaatga catggagggt ttattatgat aaaacgaaat tggccgtggc
     2401 ttctagcatt ggtcttgcta tttctctttg tccgaaacaa tagtttttct tttgttctag
     2461 gacttttatt gataggtgta gttttagttt tgtggggact ctttggttcg cgcaggaaaa
     2521 agggaaaaca agaagaaatt cctgcactga ctgacgaact tgaaactcac tatgaaaaaa
     2581 gcggtatgac agcgaatgaa atttcatttt ttcgacaaac aatgaatcaa acaaaaaatg
     2641 agatcaatca attgcaaatg aatatgcagc agacagccaa acttaaagca atcgatctcc
     2701 gccatgatcc agtcaaagca gcgaaagctt tattcaaaga attggtgaaa gaaccaaacc
     2761 gactgcatga agcaagtcag tttctttata cgcatctgcc gaatctagtt gatttgacaa
     2821 ataagtataa tgagatcaat gaccatgaga tcaaaaacaa acagacctat gaaaaactag
     2881 aagaaagcgc gcagatcatc gaccagctgt cagctttaat cgcacaagat tatcaaaaat
     2941 tcgttgctga ggatctagat gatatcgacg tagagatatc tgttgcgaaa cagagcttaa
     3001 agcgtgataa taaatatgaa ccagaacaaa aggattaact cttttgttca taaaagaaga
     3061 ctcggaacat tcagatgatg tctggctgtt tcgagtactt tttctaaagt agagacattc
     3121 atgtaagaat cgagaacaga ttcaacaagg aggaattttt tatggatcat aaaccgactg
     3181 aaacaaaaga tgttacacca gttaacgata cacttgaaga tttattgaac aacccgtttt
     3241 caactccaac agatacgctg accacttcac aacaagcgga gattgatcag ctccaagagc
     3301 aacagacagc tgctcgttta attgacaaac taccggcgga acgccaagaa caagctaaac
     3361 agctagcagc aaaaatcgat gctaatgatg cacagtctgt catcagctac ggatcggctg
     3421 cccaagcaaa attaagtgaa ttctcacaat cgatgttgaa tcatgtgcaa gcacaagata
     3481 ttggtcctgt aggagattcc ttaactgaac tgatgtatcg tctccaagaa gccaatcctg
     3541 acgaactgcg tgccggagaa gggaacatct tccagcgtgt cttcgggaag gtcaaacaat
     3601 cgatctatga agtgacagca aaatatcaaa agattggtgc acaaattgac aagatttcag
     3661 tgaaattaga taaagaaaaa gacggtctgc taaaagataa tctgatgtta gaacagcttt
     3721 accagaaaaa caaagactac tttgatgcac tgaatattta tatcgctgct ggagaattga
     3781 aaatggaaga acttcaaaca acgatcattc cagaagcgat gaaaagagca gaagaaactg
     3841 gcgatcagat ggatgttcag attgccaatg attacacgca attcctagat cgtctggata
     3901 aacgtacgca tgatcttcgc ttagctagac agatcacgat ccagcaagca ccacaaatcc
     3961 gtttgatcca aaacacaaat caggccttgg ctgaaaagat ccaagcatct atcgctacag
     4021 ccattccgct gtggaaaaac caagtcgtga tcgcattgac cttgcttcgt caaaaagatg
     4081 cagtaactgc tcaacgccaa gtctctgaaa caacaaacga cttattgaag aaaaattcag
     4141 aaatgctgaa aatctcagcc atcgaaacag cgaaagaaaa tgaacgtgga atcgtggata
     4201 tcgagaccct tcaaaccact caaaatgatt tgatcgaaac aatccaagag acattgcgta
     4261 tccaaaaaga aggaaaagaa aaacgtcgtc atgcagaagt agagcttgga cacatggaag
     4321 aagatctgaa acaaaaactg ttggatttaa cccaatagat cacctttcgc ttgttactaa
     4381 aacaaacgcc caacaacagt ttattacaac tgttgatggg cgtttttctt atctttcttc
     4441 taacttctcc attggagtct atttctcatt catcttgctg tgtccatagc catagccaaa
     4501 ataaatgatc agtccaataa ccattacgat accgaacacg atccatgtca cagtttgcag
     4561 acgaagcatc aatacaatac aagtcaaaat ggagacgatt ggcaatagcg gaacgaatgg
     4621 cactttgaat tctccagctt ttggtttgcc aaacattttt ctcaacttca gtaagcctag
     4681 actaacaagc atcaaatagg cgagcgccat gatattcgtt agttccgcta atacattcaa
     4741 tgggaaaaat ccggcaaaga atacagctgc cacaccagcg gcataagtcg cttttttcgg
     4801 tgtacgatgc ttttgatcga tttctgtcat aaacttagga agtaagccat ccttgctgac
     4861 agcaaacagt aaacgagcaa gtgaatacat catcgagatc gttaccgtca gtagcgttaa
     4921 aattgcccct acagcgatca ccgcgcccac tgttccatgt cctacatacc tcattgcaaa
     4981 agccactggg tcattgactc ctagttcagt atacggaacg attcctgtca agatcagcgt
     5041 cacgatgatg taaaggacag tagcaatcag tatcgaacca atgattcctc ttggcacatc
     5101 tttttgcggg tttttgactt cctcggctgc cattgcaacg gcatcaaatc ccaagaaagc
     5161 aaaaaacaca agagctgcac catctagcac accggaaaaa ccaaaaggca tgaatggctg
     5221 ccagttatca ggtttaacga aaaatattcc tacaatgata aacaacgcga tgataccaaa
     5281 tttgacatat accatggcat tattcaaacg taatgctttc ttggcttcaa gcgacacgat
     5341 gtaagtgacg aatatcacta cgagcaccgc aatcaagtca atgtatgtac cattttccgg
     5401 attgtacccg ccagttaatg cttgtggaag ttcggcccct aaacttttca aaaaaccttg
     5461 catatagcca gaccatccgg aagcaacaga ggaaaccgct agtaaaaatt ccccgatcaa
     5521 caaccaccct gtcagccagg cagcaaattc accaaataca acatacagat aggcatatgg
     5581 cccaccaatc actggcactc gcgaagcaaa ttctgcgaaa cttagtccag acaaaatgac
     5641 aacgattgca gctaagacaa atgaaagtga cagtgctgga ccagcatttt gattggccgc
     5701 aacccctgtc accacaaata ttcccgtacc gatgatcgca ccaattccaa gcatgatcaa
     5761 atcagacgtt tttagttctt ttttcatctc actcgtatga ttgagattga tctctttctt
     5821 acgaaacaaa tccattgaag ttcctcccca ttttaggttg gaaaacagcg attaaactgt
     5881 tttcatatac cctaaaaggg ataccaccta aaatgactaa aaggcaacca acaataacat
     5941 aaatgagctc ttttttcgtt tttctttcgc caaggatgaa aatcccacct aaagtagaga
     6001 taatgatccc catttgtgaa agtgagaagc caacagctaa accaagtgat ttgactgtta
     6061 gaagcatgca gatgtttcct aagccccata ataatccaca gatcatgttt ttccacacaa
     6121 actgatcgac tttaacttta cggaaagcaa agaagcttgc gcctaagatc attccgatac
     6181 tttgaggtaa gataattgac atcgcatcca aattcgccca gttgacgatg atggtatata
     6241 caccataacc aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa tcaagcattt
     6301 tattatctgt atctattttt ccttcgtcat cttgtcgggc agtcaaatac gcaccgctta
     6361 caagaagagc taaagcaata ataccgtata cataatcttt cgtttgtgtc cattcatgga
     6421 agaaaacggc tcccgcaatc gtattcaaaa ttaattgaaa accagtagac aatggtagcc
     6481 ctacagaaac acccatgtac ttcattccat ggaactgtcc attttgtcca acactccaag
     6541 ctaagccgga taaaaatccg atcaagaaaa tccaaggggt gattacagga ctaacaacga
     6601 aaaagacaac taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt tgttgattgg
     6661 catcgccacc aattttacca cttactaaac caatactacc ccatgccaac atagggacta
     6721 atgctattaa aatttccata accaatctcc ttttatttca catgataaga aacttatcag
     6781 cgaaaacgtt aacaacacaa gcactttttt tcgtttctaa cgattttcta acgtttttca
     6841 ttataaaaat ttaagattgg gaaaaataaa gtcattgttg tcccttttcc ttcagttgat
     6901 tcagccgtca aatggatacc taatttttcg gccagatttt ttgctaaata aagtcctaat
     6961 cctgttgaat gtgttttgct aagtcgacca tttctcccag taaatccctt atcaaagatc
     7021 cgtttcaagt cttccgctgg gataccgatc cctgaatctt taactgaaag gctgactcct
     7081 tgcgcttcct tttctatgct cacaatgatg tttccgtgat ccggcgtata tttgatggca
     7141 ttactaagca attggttgaa gataaaagcg atccatttag catcggtcaa aacagtatga
     7201 tcctcaccaa cgatctcgta gcgaatattt ttttggataa aataatttgc ctgactgcgt
     7261 atcacaggct gaatgattcc ttttagtcca tattcttgga tgaggtagtc ccgcgagaaa
     7321 ctgtcgagtc ttgcataata caaaacttgt tctacgtatt catcgatttt tgataattca
     7381 ttttctagta atgtatactt ttgatcgtca atatcgaact cgatcgaacg aactagcaat
     7441 ttagaagcgg aaagtggcac tttgatttca tggacccatg agtcaatata atctttttgg
     7501 tcttgttgat tactgattgc tgattgcatt acttcttggt gttcacggat cagctgattg
     7561 atatattctt ctaccagttt ttcttcttct gtcctcccac cactcaaata attttgcaaa
     7621 ggggaagttt tttgtatgtc ggctaatttt ccccaccatc ttctcttgct caagtaacca
     7681 cccagcagat aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc aattgtgctc
     7741 aaatcaactt tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc aataaaaaac
     7801 agccatccca agatcaatgt ccactgatct ttcaaatatt taaaaaaatt catcttaacc
     7861 gctcatcctt tcagggaacg atatatccct gtcctacttt tgtttcgata tatccttgga
     7921 tacctgcctg ttcgattttt cggcgtaacc gattgatgtt gactgtcaat gtattgtcat
     7981 ctacaaatcg ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt aagatctttc
     8041 cattgtgctt catcaaaata aataatagac gatattcatt tttgctcaaa tcaatgatct
     8101 ctccgttgat ttccatactt ccgttgtcaa cattcaacgt aatgccattg tggctcatca
     8161 tttcactaga taattctgtg tagttgtaag aacggcgaag taatgcattg atttttgcaa
     8221 tcagtacatc gatttggaaa tgttttgtca caaaatcatc tgcccccatg ttcatagcca
     8281 tgatcatatc catattcgta ttcctgctgg agataaaaat gatcggtact ttcgatactt
     8341 cacgtatttt ttgacaccaa taatagccat ccaaaacggg aagattaata tctagtaata
     8401 caagctgtgg ttcttctctt tcaaagtctg agaacacttg attgaaatcg gtcgttccaa
     8461 aggtttcaaa ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg atctcttcgt
     8521 cttcgactac catgattttt gccataaaaa atccctccat ctttcttctt gctactacaa
     8581 tatcaaagaa gcccttgtga aacaagttgg cttacctgga aaatgtcatt ttagcaaacg
     8641 ttaaaaaaaa ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc agcttaaatt
     8701 tattatgtta ttcttaataa ttggtctatt atctgttatt tttctttcgt tgtttttgtc
     8761 ttttaaattt tcgataacac caataaccga aaagcgttag cactagcaat atacctagaa
     8821 ggagtataag taatcgataa agatggtatt tttttgtctg ttctatcttt gaagagactt
     8881 cttctgtaaa gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc acggtgtaag
     8941 gtgtacaagt gataagtgtg actagatcct gcttttctct aattgctaat gtatttgttt
     9001 ttgttggaag gacaacaatt ttctcaacga cttcataagc gagcatccga ccatacactt
     9061 caagatagaa tcggtcatct tgctccattt cttcaagatc cgtaaagagt tttttctcag
     9121 caataccgct atgggcggta atcacggaat gtgtactctc tcctccagtt ggaaaagagg
     9181 tgccttgcag gactgtcgct cctctatcta acaaaagatc attcgtttca tcaaataaag
     9241 gcaagctaac attgattttc ggaatataga tggctccgat catatgttgt tcgtaatatt
     9301 cttttcctgg attttggaca tttctaaccg cttgctcaaa aggatctttg acttgtccca
     9361 ttcctgggat tactgatgta tgagcaagcg tctgattttt cttttcatgc actgctaatt
     9421 gttgctcatg tttttcttct ttatcgcgtt caagctgctt ttgataattc tcgatccttc
     9481 tttgatccaa ataattgctg agagcatctg ctaaaaatgg atatgtaaaa actgcagctc
     9541 cggcaagaaa catcactgcc ataaataatt gtaataaaag actgatttgg gtttgaggtc
     9601 tattacctat tttctttttc attttcttca ccttcttatt taaaaaaact gcagcaacct
     9661 ttataaaacc gattgctgca gcttattttt gttgcttata cttcagactt cattttagat
     9721 ttacggtacc agctgtacgc tccaatcatc aaacttattc cgattagtaa gaatgccaag
     9781 agcccgttcc caccagtagc aggaagtgac ccgcgtttca tattcggtac tctttctggc
     9841 cctggatttt ctactcctac gatctgaatt tcttcattat agcttccata agagatgata
     9901 aattcagtaa aggctgcctc acctgttgga agtacataac cttctggcgc catggtctct
     9961 ttcatttgat aggttccata atctaaccct tgaacctcaa agtatccatt tccatctgcg
    10021 acaaattctg ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc tgattcataa
    10081 acagctaaag gcgactcccc tttataaaat attgcgtatt cagaacggtc ttcattccac
    10141 aattcgaatc ttgcatcttt aagcaattca ctgcttgatg catcatattt ttcaaacttt
    10201 cttccacctg ttgtgacttt ctctggcggc tctactcctt cttcataatc aaattcatca
    10261 tttcgtccga tttcaataac cgctgtgttg ttatggaaat catccgcttc tagttctgtg
    10321 gaaaccttca aattatacgt gatcgttaaa tctcctccac gtgcatctgc taatctacct
    10381 aaagtatcta ctttggctac tccattttct tctgtcaaaa tcgtcacgac aaacccgttg
    10441 cttactactt ctactatata atcttgtcct tttgttaaaa tcgtctcgcc ggctcgcact
    10501 tctatttcct gatcataata agttagacca actgtaggcg tatctgtgat tttgtaattt
    10561 ttgataacag cgcttccgtt ttcaaggaca gaatcgatga aataaggaat aggtgctgtg
    10621 atcgtgtaag aagccgtttt tcctctttct agatcaatat aagaaatcgt ttcttgttct
    10681 ccagcttcat taataatcgt tacttgtcta aggtcgcttt cctctacatt catctgttta
    10741 tctgcttctc tgatttcgtt tttcggatac aaatgcacat ctgtattgta tgtgtctttc
    10801 catgtagaac catcccacac gccatccacc atatcaggca tcatcactgg catcataaca
    10861 accattggag acgcgattct ggaaatatga gctggcgaat aagtttctaa gaaaagatag
    10921 actttatctc tagaagaagg tgcttcttcc gtcacttgga cggtagagaa taccgcttct
    10981 ccagcttcat tcgttgtttg tttgtcaata cttgtcagtc gatattttaa aaaccaactt
    11041 tctgtgtgcc gtttttgaat ccattcaatc gcttcggcag aattaagacc gtcttctcgt
    11101 tccggtgtta aaggattatc tcgaatcaaa tcgtaatata cttccgttac gtcaaacaaa
    11161 tcaaaatcga ctcctggaat attttcttca ccaaaatccg tagaaaccaa tccgctattc
    11221 ggtcgttctt ccggaacaga tgagaaacct tttttgtgta atgtaatcgt cacttcactt
    11281 ggtttttgtg cgacttcttc ggctttgact agttttccgt tcccgaaaat ccctattccc
    11341 gcagtaacga gtaacagtac actcattccc gtgacaatca tttttttatt catttttttc
    11401 tccccgttct ttttttatta ataaaacagc acctaacaat aaaagaattc ctagtaaaga
    11461 aatcaacgtt cttgcttcat tcgtttgcgg gaatacggac cattttcctg attgacctgg
    11521 attgcttgga ccactcggct ctttcggttt gtccgttgtt tcttttacgg tctcgttata
    11581 aacacgtggt gttgcggatt cataggcaga gactggaact ttcccggatt ctaattcagc
    11641 catctcctgt ccgttaacta ctacatattg tggttggcct tttgcatcca cccaaaattc
    11701 agggatgatg acctcgattc tcttacttgc ttcatccatc tcgtaacctt ccacaccttt
    11761 taattcttca aaataatagg tgccagaagg caagaaacgt tgtcctgtcg taacaaggcc
    11821 ctctttatcc gaaatgaatt tgcttacatt tttgtcattc aaaggatcat tgtctgctgg
    11881 tttgatccat tgatttttca agtcattagc aggagccata tctaaataat atttcccccc
    11941 ttctatcatc tgatatagag caaaaacagc accttctaat ggttttcctt tttcagttgt
    12001 tcctctttga cgtccatact tgaagaagta tggatctctt agataaccga catttttagg
    12061 atagatatga atctcttgca gttcctcggt tgattctgtc gggtgataaa ttggcaaaat
    12121 agcagcaatt gggatagctg tctgatcgat atcaacatcc aatccaactt ctggatcaat
    12181 cgattcttcg aaaatcaaat aaactgcatc ccgatcttgg tttttcttag gtgctttgat
    12241 acgtgcaatc ccgtctccta atccgccaac cgtatctttc ccagttgtta ccgaagtccc
    12301 tatttggaca agcctttctt ctgtgattag ctgacggata ttgtttctgt tcgtttcagc
    12361 gatttgctgg acgaattctt taggagtcat gttttcagca gctttttttt catgataata
    12421 atcggtcatg tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct gtgaaatgat
    12481 atctgctgtt tcccctgctt cctcagagat agccaagcca tcattttcat ggtattcaaa
    12541 ttgatcgtta taatcgatat ctcgaatcat ccgtttatgc aacacgaatt ccacgacatc
    12601 ttcccctgat tcttctgccg acatcgcatg tggaagaagg actgctgcaa gcggtagtaa
    12661 aacaagtaat tgaagcatca aacacaacag ctttttcatt accttgctcc ctccttttta
    12721 gattgtctga caaacaaacc ataggctgct cctgcaagcg ccatgaagaa tgcagcaagc
    12781 aggatataga ataatcgtcc ggatcctcct gtagcaggca acggattttc aggatcgttc
    12841 ccgacactaa gctgtaatct gattcgattt gtctccaaaa gttcatattc taactcttgc
    12901 tgtagatcag gcgtctctgc ttcttcgttt ggataatacg aaatctcaac ttttagtgta
    12961 cccgcttctt ttcctggaac tacccgatca ttttctatgt caggttcttc tcgataacta
    13021 atttctaaaa taaagtaacc tggaagtttt ctatagctgt caggtgactt cacttccttg
    13081 atccgataga ccccttccaa caagtctgaa gcattgaata gggaagaatc gtttggatct
    13141 ggtgccaagc ctccctcgat gatttcatac tctcctgcac tattttgttt ctcaattgaa
    13201 aattctgcgc cttcaagacg tcggctattc atactgtcaa ttttcaaaag ttccaaatcg
    13261 attggcgcta gttcatcttc taaggttact tgtagtacac catcgatgat ttcatagcct
    13321 tccggcaagg gagcagtttc gtcttcgatg accgtatctt caatgataaa cttaccttct
    13381 tcattcagtg caaactggaa cacttgatca gatagccgat acccatttgg tgcgacagat
    13441 tccgagacct catattcacc aatagacaga tgcagaggtt ggtcgtaatt taaatcgaca
    13501 tacttttcta ctcctacggc acgagaatag aagtatccga tcccaggcaa acgtttcgat
    13561 tcttctggtt ctaacggatt accagtgtat gctcgataca gcgggaagga agaatccccc
    13621 actttcctga tatcgaaacc tgcaccatct aatgtatggc cttcatggtc taattttcta
    13681 actcggatct cccgataatc aaacgtattg taagcagtga atgctaccca taaacgatcc
    13741 tcttcatctg ttgtatacgt gagatattgc caattttctt cacctgtttg cttgagttgg
    13801 atagtcccta tagcattatc gtaaagtcgc aagatccatg tgtcattttc atcaggttct
    13861 ttgaaacctt ctgggttctg gctctcaatg attttgtatt ctgcatagtt ggtagtttct
    13921 tcactatcct tataaacaaa tgaatctggc tctccgattg gatgaataac tttctcacca
    13981 tccacgatta catatttccc gtcttcatct actgaataga acgtgagtct ccctttctct
    14041 tcctctactt cgttcacacc agaaagccct ttcgctactg gatatccgat ttccagtccg
    14101 ttaccatctg tttcataaag ttcaaactct gcgccattga ttggcatatt actgccttca
    14161 attagtttat tgatattcat gtggattggc ttgaattcat ttcgcatttc cagtttgaca
    14221 tacaactcat catactctgt gccttctatg ttaggctctt gtacaacaga aagcttgtga
    14281 tattctcctt cataatagga attcggatct ttaccttccg gtccgccgac tctcgaacca
    14341 gatgtatatt catctacaag ctcgtagtga cctaacatct catcttcgtt ggctgcatat
    14401 tttttgattt ggaattgata cgtctcatct actaactgat aacgtggtgg tccagcgatc
    14461 tctctgatcc aaaatatgtc gctgactgct ccagtttcat ctcctaaaat cggcatcttc
    14521 gtttcaaaga tcgctattcc gtccgtggta gttttacttg tttcgattgg ttcggcttcc
    14581 ccactctctg gattttggtc atataatgca aattcgactc cagccaacgg ctcgccattt
    14641 tgacccttct tgctgacttg gaaattacgg cctttatgtt cgtaacgaat ggaagaagat
    14701 gggaaataca tccgtccagt agtgatatca ttctcataat cctccaggtt ctgaatatag
    14761 gccatattgt tcactgtgca aaaatttccg tcttgagctc ccgcatcaag ttttacttgg
    14821 tacttgatcc tgaccatttc cccttcacct aaagaaatcg gagaaaattt ataagcttgg
    14881 ttttcccgtc cgacatacgt tgtcggagtc gccactactt ctggtggggc ttgtccatga
    14941 cgttcttcat cccatatttc tggctcttgg tacttcatgc cgttttctcc tggatctaga
    15001 tggtaaaact gaatatctgc cgatctgcca gctccaaatg gtgagacatt aaaaccagtt
    15061 tcatcataaa gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat atcctccaag
    15121 gctgtaacaa tgtcattctt gttgttcgca ttgtaatatc gcggtgttac gccatcttct
    15181 tctaaatcag caatattttt caatacattt cgtccaatag catctagtcg ttgtctcccg
    15241 ctaacgtttc catcgattcc taaaccaatg gagaagatac ttggcgggtt ctcttgatac
    15301 ttgtttttca gccaatgatg ataagcaact gtgttgatcg cagatgactt ttcttgcgaa
    15361 gtaggactgc cattcgtatt gttattagaa gaaccatcac caacccagtg agtcgcttta
    15421 ttatctcctt cttttacctc tccataacga tagtgtacat ttacttcatc ttctggacgg
    15481 ttaaagtcag atgcataggt aactggatga gtaaaccctg cattagaagt attcccagaa
    15541 aactcctcaa aatttctgaa caaaacgtca ttatcttcag caatcttatc ttccatcaca
    15601 gtccagttgc tccaagctcc attagtgctc gtacgataac ttgactgaat cggtgcataa
    15661 ctgaaggtcg gtgtcccatc tcccacaacg attaggattt tttctggatt tctattttct
    15721 ccgccattgt ctttatacaa tgtttcatag ccatttttaa gccctagtgt aagcggagtt
    15781 cctccgattg gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg attgatatca
    15841 tttgtcaatg gaaagatgtc cgaactgatt tgctcatggt tattgatagc tgtactatta
    15901 tggtaaaaat tcaccatacc gattcgaata tccagactcg ggtcagagag cagatcttta
    15961 ctcatttcgt ttactgcctc aatcaacgca gcatttttag tttgactatt ggtcccagcg
    16021 gttaattcac tcatactcgc tgacttatct agaacaaaaa cgatatcgat tggactgatt
    16081 tcctttttct cgcccccgat gacatctaga tagacattgt aaagggttgt tgcatcttca
    16141 ggatgtagat cagatgctgt gctgcttggc agaacaggtt ctacccattt tttgatgtac
    16201 gccccgttat aatccaagta actgttttca aaattccgtt catttcccca aagctgaacg
    16261 gtttgagctg gaacgtttct atcttcttcg cctgcagttg atccattatt agtagcatag
    16321 tcgtaatttc gccagttcgc tcccccgttt gtataatcac tatcatcgat caagttctct
    16381 ccgtcatatg gcgagccttt gtctccttga tagcgttcat tgctttgtgt ccaatttcct
    16441 aacacttttg tatagtcatt cgttgtaaac ttcgggtagc gatgctgttc gttttcaact
    16501 tctccgctag gatagatata ttcatagggg tcttgcgtag ctgttggccc aagacgctcc
    16561 aaaataccgt tgaataactg gcgttgaatg cgctggagtg cttgaccgct gggtacgatt
    16621 ggttcgtcca gcttttccat ctcattttct tcgctacgtt gaatttcagc ggaatcttct
    16681 gtatcagttt cattttcttg taattcttgg ttatctattc tttcatcaac caccacttga
    16741 agaggtagtt acccttcgtt cacatcgggg atcttttctg tttcaaaaat caatgtgccg
    16801 gtttctgctt cttttgaata aacttttccg acataccatt cttgaccatc gatcgtctcg
    16861 acttttaggt cttctccatc ttcatcatag gaggatagac ctgttccacg aacgtttttt
    16921 acggtaccaa tcccggcggc tgccgcatcg attgctaaac gtattctacc ttcattggca
    16981 ctttcctttt tttcaaaagc cagatgccat tcaagggttt cttctttttc aatatacttc
    17041 ccttcaattt ttgcatagtc ctcatctaag aatacctcat cctcctttgt ttctgttgac
    17101 tcagcagcta catccagggt cgtcatatga tagactgact ggaaatatgg tccaaatagc
    17161 aaactgacag ttactaactg caaaagcttc aattttaacc ctttcacatc cttatactcc
    17221 tttcaaaaat atttgtcttc tttcacaaca atcaaca
//
LOCUS       DASJTC010000062        16890 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.062, whole genome shotgun sequence.
ACCESSION   DASJTC010000062 DASJTC010000000
VERSION     DASJTC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.062"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(247..1974)
                     /locus_tag="U4B19_001869"
     CDS             complement(247..1974)
                     /locus_tag="U4B19_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEN2077601.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(1967..3160)
                     /locus_tag="U4B19_001870"
     CDS             complement(1967..3160)
                     /locus_tag="U4B19_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEN2077602.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3347..3991)
                     /locus_tag="U4B19_001871"
     CDS             complement(3347..3991)
                     /locus_tag="U4B19_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2077603.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4085..4219)
                     /locus_tag="U4B19_001872"
     CDS             complement(4085..4219)
                     /locus_tag="U4B19_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEN2077604.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4221..6353)
                     /gene="feoB"
                     /locus_tag="U4B19_001873"
     CDS             complement(4221..6353)
                     /gene="feoB"
                     /locus_tag="U4B19_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEN2077605.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6350..6823)
                     /locus_tag="U4B19_001874"
     CDS             complement(6350..6823)
                     /locus_tag="U4B19_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEN2077606.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7224..7448
                     /gene="nrdH"
                     /locus_tag="U4B19_001875"
     CDS             7224..7448
                     /gene="nrdH"
                     /locus_tag="U4B19_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEN2077607.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7607..9766
                     /gene="nrdE"
                     /locus_tag="U4B19_001876"
     CDS             7607..9766
                     /gene="nrdE"
                     /locus_tag="U4B19_001876"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEN2077608.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            9816..10781
                     /gene="nrdF"
                     /locus_tag="U4B19_001877"
     CDS             9816..10781
                     /gene="nrdF"
                     /locus_tag="U4B19_001877"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEN2077609.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(10870..12009)
                     /locus_tag="U4B19_001878"
     CDS             complement(10870..12009)
                     /locus_tag="U4B19_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEN2077610.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12318..13031)
                     /locus_tag="U4B19_001879"
     CDS             complement(12318..13031)
                     /locus_tag="U4B19_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEN2077611.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13285..13986)
                     /gene="nagB"
                     /locus_tag="U4B19_001880"
     CDS             complement(13285..13986)
                     /gene="nagB"
                     /locus_tag="U4B19_001880"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEN2077612.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14220..15752
                     /locus_tag="U4B19_001881"
     CDS             14220..15752
                     /locus_tag="U4B19_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEN2077613.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(15943..16122)
                     /locus_tag="U4B19_001882"
                     /pseudo
     CDS             complement(15943..16122)
                     /locus_tag="U4B19_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(16142..>16890)
                     /locus_tag="U4B19_001883"
     CDS             complement(16142..>16890)
                     /locus_tag="U4B19_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HEN2077614.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKL
                     ALKGITISVPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLES
                     RFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         5220 a         3475 c         2966 g         5229 t
ORIGIN      
        1 aatatttttt gagaatgcac ttcgtacata ataaacttta atcacattag tctttcatac
       61 ctacgatcga tctctttgac tcgcacccat aaattcataa tagtttgtcg gcgtctttga
      121 acgtttcgaa gatgagctac gcattctctt tttttataga caaaaaagat agagacgaac
      181 atggagcttc caaatttgga tatttgatcc aattttggaa gctccattta tttcgtgtct
      241 gtctttttat tttttccagc tttcgactaa ttcttggttc tctttgatcc agtcttttgc
      301 tgcttgctgt ggatcttttc cattgttgat ttccaacata actttttcca tatctttttc
      361 tgaccaatgg aaattgtcta atactttgta agcttctggc tggtcttctt tcaatccttt
      421 tcgaaccatc gtatggatct gctcttcgct tcccattccg ttttcaggat cttctaagta
      481 tttcagatca tatttggcaa acatccaatg aggtgtccat cctgtaacta caattggttc
      541 gtgttttttg atcgcttgac ctagtgcaac ggtcattgct cctgaagaag atgtctctac
      601 tttccagtct ttcaagttgt catatttttg gatcgtgttt tcagcagccg taactacacc
      661 agctcctggc tcgattccta tgattttctt acctgcctga tcggttaact catcgatcga
      721 atttacatcc atgtaggctg gcactgctaa accaactttt gctcctgtca aattggcacc
      781 taggtcttcg acttgatctt tatattgtgc atattgtgaa ccatgagttt taggtagcca
      841 agctgaaacc atggcatctg actcgccatt tgaaacagat ttccacatga ttgaattatc
      901 taatggcgtc atcgttacat catagcccat ttgtttcaat acttcaccga ctacatttgt
      961 tgaagcaact tctgtatccc attcaacata agataagttg atacgttttg tttctttggc
     1021 agatgaaaaa ctccctatac caatcgcacc taagatcact actactacag caccaatgat
     1081 cagtccttga cgttttttac ttttctgtgt tgttgctcct gcagcttttt tattgtttgg
     1141 ctggttcaaa tgctgtgtaa aacggtcaat gatgattgct aagataacta atgcaacccc
     1201 gttgacaaat ccattcccga cttgtgctct ttgcaatgct gataatacgc cgcgtcctaa
     1261 gccgggtgca ccgatcatag aggcaataac gaccattgaa agtgacagca ttgttgtttg
     1321 attgatccct gccatgatcg tacttttagc taaaggcaat tctagtttga atagtttttg
     1381 acgacttgtg ctcccgaatg aatcagaagc ttctactaat tctttaggaa cttgtcgaat
     1441 ccctaaattc gtaaaacgaa cggttggtgg cagagcaaag atgactgagg caaatacccc
     1501 tggaaccatc ccgataccga aaaatgcaac tgctggaatc aaataaacga atcctggcat
     1561 tgtctgcata aagtcaagga tcggtttgat aatactttgg gctttactgc tttttgccat
     1621 caagatccct aatggtaccc caatgatgat cgagatcaca ctagataaaa gaacaagtgt
     1681 gacagtattc atcaaatcat tccatagacc ttggttataa ataaaccata gaccgattaa
     1741 tgtgaacaac gtaagtcctg gacgtttctt agaaataaag aaagctgcta ccgtcaataa
     1801 caagataaac aaaggagctg gaatcactaa cagtaaattt gtaatccccg acatcactgc
     1861 ctgtccaatc gtttgtaaaa atgaaaatag gccagaaaaa gttgtcgtaa accattctgt
     1921 gatattttct acccagtttg ccactggtaa ttgatagttc aatagattat tcatgttcac
     1981 tcacctcgct tgtttctgct aatgcttcaa tcacactacc tcggatgacg acacctttta
     2041 attttccatc ctctacaact gcaagtggag tcggtgagtc ataaataata ttgaaaatat
     2101 cacttaccac catgtctttc ccaatcaccg tcacattttc atctaggaag tcgatcaatg
     2161 gacgtttttg cttcctagct tctaacgctt gatctgcagt gatgatccct ttcaggtgtc
     2221 tctttctgtc aacagccatc agcatgctga cttcttcatt acgcatcctt gtcaatgcaa
     2281 cgtttggtcc atcagattcg atattcgtcg tcaatgcagg taccatgatg ttttgagccg
     2341 taagcacttt ggaacgatcg acttcttcca caaattcacg tacataatca tttgctggat
     2401 tggtcaatat ttcttcacct gttccgatct gcatgatttc gccgtctttc atcaaggcga
     2461 ttctgtcccc aatccgtaaa gcttcattca aatcatgggt aatgaaaata atcgtttttt
     2521 gaacattggc ttgcaggtcc agtaattcgt cttgcatttc tcgccggatc agcggatcaa
     2581 gagccgagaa cgcttcgtcc atcagcaaga tctcaggatc atttgctaaa gctctggcta
     2641 ggccaacccg ttgttgcatc ccaccagata actgactagg atattggtct ttaaatgaaa
     2701 gcaaactaga attttctaat gctttttctg ctttttcttg tcgttcttct ttaggtacac
     2761 cacggacttc taacccgtat tctgtatttt ccaagatcgt tcgatgtgga aacagaccga
     2821 agttttggaa aaccatattg attttatgac gacggacttc tcgcaattct tctttgctta
     2881 acttcgatac atcttctcca tcaatataga tactgcccga agttggctca atcagacgat
     2941 tcaatagacg gatcaatgtg gatttcccac tcccggaaag ccccatgatc acgaagattt
     3001 ctccttcttt tacgtcaaaa ttcacgtcat acacaccaac cgttgcaccg gttttcttta
     3061 aaatctccgt tttcggctgg tgatcattca tcatatctaa agcggcttgt gtctttttac
     3121 caaatatctt tgttaaatgt tttacttcta cttttaccaa acaaattctc cttttttatg
     3181 taaattcatt tttctttctg aaaaaagtga ccacactatg ttaacatcat gtagtcactt
     3241 tgtcaaaaat gaaagaaatt tgcttgtcca aactgaaaaa aacttggcta aatctttgta
     3301 aataaaaggt ttagccaagt taatattttt ttaatatttc acacaatcat tctgcaagct
     3361 gatgctccgg gtaaaaaaag tttgtcatac gctgtagtgt atattcattt ccaataaaat
     3421 gaacgatgtc ccctgccgta aatttagcat aggggccagg tgaaacaagc agttcttcat
     3481 tatgagaaat tgcgataact gtggcgccag tcgcctgcca gatattcaac tcgctaattg
     3541 aacggtccag atgcattgct ttttctgata aagataattc aaacggaagt aaaggattca
     3601 tcttatgtat tcgcttcgtt tgattgacca gctgatccaa taattgagaa aaatgattca
     3661 gctcatcttg ttgcttctcc acacttgcca tgatttctgc ttttgtatct tggatggatt
     3721 gtacatcttg atattgcaat aggaaatctt ttgctttttc cttcgaagca acaaatgccc
     3781 cacttccatg ccgtacttcc atgattccaa gatctaccag cacattgata gccttgcggg
     3841 tagtctcagg agaagcattg tacgtactgg caagtgttga gcgggcatgg atcttctcac
     3901 ccactttata tcggttttca acgatacgtt cggctaaatc aacagcaatc tgctggtagc
     3961 gcgggcggac gatttttttc tttccagaca ttttctttcc tctttctttt tttactttcc
     4021 ctcatcttac tatgctctag caaaataagc aaaaaagggg agaatagcct cttgcaacga
     4081 ctgatcatcg cttgatctgt tcctttttta ctgggcaagc tgtttgacag tcatcacaat
     4141 tcttaccgga ttttaaacga gtatagacaa ttgcgcctgc acttccaaag attaacacac
     4201 ttaataagaa tgtcgccatc ctattcctct ccttctaatg acaatatagt ataaaactgg
     4261 ttcttggatt ttggctgacg gaacaaaaca taaacgagtc ctatcatcag taaaatagca
     4321 agatacgtcc ctaacgctac ctgtccaccc tccaataagt gtcccaactg ataaatcaca
     4381 aaactaacta aataagctaa accgcattga tagcctatgg cgatccaagt ccatttcttg
     4441 gcattcattt cccgatggat agccccaatt gccgcaaaac aaggcgcaca aagtaaattg
     4501 aaggctaaca atgaataagc tgctgcggga gtatatgtgt gttgtagcac ttgccagatt
     4561 tctctgccat tttcggatac ttccgcatga ccaaacaaaa taccaaatgt gccaataata
     4621 ttttctttag cgaccaatcc tgtaatcgct gcgactgttc cttgccagtt tccccaacca
     4681 agcggctgaa aaacaactgc caggattcgc ccgaaaaagg ctaagatact ttggtcttca
     4741 gaaacaggtt gagcataaaa attataagaa gagctgaacc agatcacgat actagaaaca
     4801 aagataatcg ttcctgcttt tttaacaaaa gacttgctgc ggtcataggt ttgtcttagg
     4861 accgctgacc attgaggaaa atgatatgcg ggcaattcca tgataaaagg agctgtatct
     4921 ccagcaaaca aacgtgtttt tttcaagctg attccagaga aaataattgc agtcatacca
     4981 agaaaatagg cagaaggagc tacccaagat tgatgaggaa aaaaggcccc tgctactaag
     5041 gcaataatcg gcaactttgc agaacaaggc atgaaggttg tgaccatgat cgtcaaacga
     5101 cgatcttgct cgttttcaat cgtgcgactg gccatgacgc ctggcactcc acatcctgta
     5161 gcaatcagca taggaatgaa ggattttcct gaaagaccga atttcctgaa aagtcgatcc
     5221 atgacaaaag cgatcctcgc catatatccg cagtcttcca gaaagctcaa gcataaaaac
     5281 aatataatca attgcggtag aaatcctaac actgctccca cgcctgcgat gatgccgttc
     5341 aagatcagct cctgcatcca tcccgctact tgccattcag ctaacaatct gccaacatgt
     5401 tctggtaacc atgtaccaaa caatgtgtca ttgatccaat ctgtccccat tgttcctact
     5461 gtttgaatag ccagataata gatcagccac atcacaaatg cgaagatcgg caaggctaac
     5521 caacgattcg ttgcaatctg atcgattttg tcactcatcg ataattggaa agaatcattt
     5581 gacacaacac agagtgtaca tagttgggta ataaattcat aacgttcatt gaccagaatc
     5641 gtctcactat cgtcgtccaa tagtttctct gttaactgga tcagttgctc gatctctttt
     5701 tgctgagaaa ctgctatctc gtaatcctct cttaccttct gatctcgttc aaaatatttc
     5761 attgcatacc agcgtttctt gccgctttgt tctcctagta catcttcaat ttcatgtaaa
     5821 gctgcttcta aacgtggatc atatgtacag ggaacaggaa tagaaggccg cttttttgca
     5881 gactcctgaa tcttttcgat tagtttttcg attccttgct ttttcaatac gctgattggt
     5941 aaaactggta caccaagacc ataggaaagc ttttctagat tgattttttt tccttctttt
     6001 ttgatgacat ccatcatatt cagaccaatg atcataggaa tgtctaaatc caataactgc
     6061 gtagttaaat aaagatttcg ttctaaattg gtagcatcaa taatgttgat gatcagttca
     6121 ggctgttcct gaaccaaata atctcttgca atcacttctt ctggtgtata aggagacaaa
     6181 gaatagatgc cgggtaggtc ctgaatatga atgttcggat tttttttcaa cgatccttct
     6241 tttttttcta ctgttacgcc cggccaattc cctacatatt gattcgaacc agtcaagaga
     6301 ttgaacaagc tagttttccc gctgtttgga tttccgacta acgcgatatt cattcttttt
     6361 cctccctttg tatcaagatt agttcggcct ctgactttcg taacgtcagt tcataccctc
     6421 gcactgtcag ttcgattgga tctcccagag gggctagttt tcggataact atttttacat
     6481 ttcgcgtcaa tcccatatcc attaaccgtc gcttcactgc gccttgtccg tggattgcta
     6541 cgacgatccc agtctctcct gcttttaatt gatcaagaga agtccagctt tcagaggaag
     6601 cagtcttttt attgacaaag atttgaccta gtatttcccg acttactgcc actcgatgac
     6661 tttttaccag taaaatggca ttgttgcctg ataaattgac gacgacgatt ttacttccgg
     6721 caacgatccc gatattttgc aaatggcgtt ttgttttttc ttctgtttcg attcgttcga
     6781 cttgataaac gaaattctgc tttgcctgcg ctaaggtctg catattgatt cctctcttgt
     6841 gaaataaatc atgtattaaa atcattcatt atatgttcta attttcttac gtgttacgca
     6901 aataaatgag aatcattctc acatagaata ctctcggtaa ttgagaatgt caattatact
     6961 tgtgatatta tgagcgaatc tttagtattg atagataaaa atgagaaaaa aatatttctt
     7021 catagttttc cacaattttt tctctattaa atttgctatt ttcccacacg atacttgttt
     7081 tcagctcttt tttcaaaaac acaaaactta gtattccaca gtttccacaa cacaatatgt
     7141 agtgtttgtt gtttgacttt tttactaaga gttttatact atctattata gataataaga
     7201 atgtgttaga ggaggcactc accatgaacg taaaaatctt ttctaaaaat aattgtatgc
     7261 aatgtaagat ggtcaaacgg tttttaacag aaaatcaaat tgcttttgaa gaagtcaata
     7321 tcgatgaaca gcctgaagca gtagactggt tgaaagcaca aggattccaa agtgttcctg
     7381 tcattacttc tgatgcagaa acagttatcg gattccgtcc tgatcagttg cgtaaacttg
     7441 ccagctaaca tgactaaaag aatcaaaacg tgatacatgg atttgaaggg cttgagacag
     7501 caccagtttt tgtcgcaaac ttccaaatcc ttttttaaag cttaaaaaca atgcaatgat
     7561 ttgcaatcaa gcatcatcaa atacgaaatg aaggttgggc gtacgtatga gtcttaaaaa
     7621 tttacaagat gtcagttatt tcaagctgaa taatgaaatc aatcggccag tcaatggtca
     7681 aatccctttg aataaggata aagaagcttt agttgccttt tttgaggaaa atgtcaaacc
     7741 aaatacgatg acttttccat ctattatgga aaaaatcaat tacttgatcg atcaagaata
     7801 tatagaagca gaattcattc aattgtattc accatcattt attgaaaagc tatatcaatt
     7861 tttggacgag caacattttt ctttcaaatc ttttatggct gcttacaaat tttattctca
     7921 atatgcgttg aaaaccaacg acggttccgc ttatctggaa acttatgaag atcgagtagc
     7981 cttcaatgcg ctttactttg cgaacgggga tgaagaatta gcacttgctc ttgcagacga
     8041 aatgatccat caacgatatc agccagctac accttctttc ttgaatgctg gacgtaaacg
     8101 tcggggcgaa ttggtgtctt gtttcttgac tcaagtcact gacgatatga actctatcgg
     8161 gcgctcaatc aactctgctt tgcaattgtc ccgtattggt ggcggcgtag ggattacttt
     8221 atcaaatctt cgtgaagccg gcgctcctat caaaggatat gagggtgctg caagtggtgt
     8281 cgttccagtc atgaaattat tcgaagatag tttcagctat tctaatcaat taggtcagcg
     8341 tcaaggagct ggcgtggttt atttgaacgt tttccatcca gacattattg ctttcctttc
     8401 agcaaaaaaa gaaaatgcag atgaaaagat tcgcgtaaaa actctttctc ttggcgtgat
     8461 cgtacctgat aaattttatg aattgacacg taaaaacgaa gatatgtatt tatttagtcc
     8521 atatagtgtc gaaaaagaat atggtgtccc attctcttat gtaaatatta cagctgagta
     8581 tgatcatcta gttgcaaatc caaacattcg caaacaaaaa atcaaagcgc gtgatcttga
     8641 aaatgaaatc tcaaaattac aacaagaatc tggctatcct tacgtaatca atattgatac
     8701 agcgaaccgc gagaatccaa tcgatggcaa gatcatcatg agtaatttat gttcagagat
     8761 tttacaagtt caaacgcctt ctgtgattaa cggcaaacag gaatatgaag tattaggaac
     8821 agatatctca tgtaacttag gctcgacaaa tatcgtgaat ttgatgcaaa gccctaactt
     8881 tggtcaatct gtgcgtacga tgactcgtgc attgacattt attacagatg catctgatat
     8941 cgatgtcgta ccaccgatcc aaaacggaaa taagctgaat catacgattg gattaggtgc
     9001 catgggctta cacacttatc ttgcaaaaga acaaatggaa tatggctcag aggattcatt
     9061 agactttaca aatatttact tcatgctgtt gaattattgg acacttgttg aaagcaacaa
     9121 tatcgctcgt gaaagaaaac aagttttcca taattttgaa aaatcagctt atgctgacgg
     9181 cacttatttc gaaaaatacg taactggaga attccagcca aaatcagaaa aagtcaaagc
     9241 attgtttgaa ggcattttta tcccaactgc tgaagattgg aatgctttga aacaagcagt
     9301 aatgaaagat ggactgtatc atcaaaatcg cttggcagta gcaccaaatg gctcaatttc
     9361 atatatcaac gatacgagtg caagtattca tcctatcact cgcatgatcg aagaacgcca
     9421 agagaagaaa atcggtaaaa tttattaccc agcgccttat ctatccaatg agacgattcc
     9481 gtattacact tctgcttatg atatggacat gcgtaaagta atcgatgttt atgcaacggc
     9541 acaacaacac gtcgatcaag gcatgagttt gactttattc atgcgttctg aaatcccaga
     9601 aggcatttat gaatggaaga caacaacgaa gcaaacgaca cgtgatttga atattttaag
     9661 acattacgct ttccacaaag gaatcaaatc catttattac gtacgtacct ttactgatga
     9721 tgcagaagaa atcggaagca atcaatgtga aagctgcgtg atctaatatc atagatagat
     9781 tcgcttattc atattttaat taaaggagca ttctcatgtc agaaacatat tatgaagcaa
     9841 taaactggaa tgaaattgaa gatatcattg ataaatccac ttgggagaag ctgacagagc
     9901 aattttggct agatacacgt atcccacttt caaatgacct agatgattgg cgtacattgt
     9961 ctcaattgga aaaagataca gtaggacatg tatttggcgg tttgacactg ctagatactg
    10021 tccaatctga aagtggcatg gatcagttgc gtaaagatgt ccgcactccc catgaagaag
    10081 ccgttttgaa caatattcaa ttcatggaat ccgttcatgc caaaagttat tcttctattt
    10141 ttagtacact gaatacgaaa aaagaaatcg aagaaatttt tgactggaca aatacgaatc
    10201 cttatctgca aaaaaaagct gagcgtatca atgaaatcta caaacacggt acaccgctag
    10261 aaaagaaaat tgccagcgtc tttttagaaa cattcttgtt ctattcaggt ttttatactc
    10321 ctctttatta tttaggaaac aacaaattgg ctaacgtagc agagatcatc aaactaatca
    10381 tccgagatga atctgtacat ggaacctaca tcggttacaa attccaatta ggattcaacg
    10441 agttacctga agcagaacaa gaaacgttaa aagactggat gtacaatctt ctttatgagc
    10501 tttacgaaaa cgaagaacgt tatacagaag aattatacga tccaatcggc tggacagaag
    10561 aagtcaagac attcctacgc tataatgcca acaaagcatt gatgaaccta gggatggatc
    10621 cactctttcc agatactgcc aatgatgtca acccaatcgt aatgaacggg atttcaacag
    10681 gcacaagcaa ccatgacttc ttctcacaag tcggaaacgg ctatttgtta ggtcatgtag
    10741 aagcgatgaa agacgacgat tatttgattg gattggaata aggatttatt ttaattcaaa
    10801 aatttttcga aaaataaaaa gagtttagcg tacagatatt ttaaatgtac actgaactct
    10861 tttttatgct tattcacttg tttcttccag cacttttgga ctgtctaact tttccgaaac
    10921 atgttccatt cttctcaata aatagatttt ccggatattt aatattatcg tcttaatcac
    10981 tgcatagaca ggtatgcaaa tcagcatgcc taatattcct gcgacacttc ctgctccaat
    11041 caataccaag atgatcgtca aaggatggat attcattgat ttcccaaata acaacggctt
    11101 gatcaaattt ccatctaatt gttggatcac taaaatcgac aacgccatat acaaagcttg
    11161 aaatggagag ataaacaaac cgacaatgat tgctggtacc gctccaataa aagggccgac
    11221 atacgggata atattcgtga ttccgcaaat gaccgcaagc aacaagctat aaggctgatc
    11281 gaatattctc attgccaaaa agctcattac accaataatc aaagcatcta agatcgtact
    11341 gctgatatag gcagataatg tttcgtttaa ttcttttacc gtttggcgta actctttacg
    11401 aatcgctttc ggaaagaagt gagacaacgc atcaataaat ttgtgcccat ccttgaacat
    11461 aaatagcaaa ataaatggaa cggtaaataa caaaataaaa aattgcatca taaatgaaat
    11521 gatttttgag acacttgcag ataaactgac taatatgata ttgaacaaat tactaagaga
    11581 taaatttgct gatgctaatt gttcttccaa attgaaattt ttaaactctt cccgctgcgc
    11641 caattcattt aaccatttgc ttgtttcatc tgcatacatt ggcaagctct gggtcaattg
    11701 aattgtctgt tccattaact gaggcactac attcatgaca gttaaaacaa ctaaaatgat
    11761 tacgaccaaa aacgtcaata aaaaaccgaa cactctcggg atccgatgct tttcgagaaa
    11821 aacaacgatt ggatcgaaca tataatagat aaaaccagca acaagcaatg gtaaaaaaat
    11881 tgccgaaagc attttcaata ttggactaaa aatattcggc atttgtaaaa taaagtacac
    11941 gccgatgatc gtgagtacta gctccactgt ccagaaaaat aattttgatt gtttaaaacg
    12001 tgattgcata catctcctcc aaggtccttt gatacttcta ttctatcatt taacaaagag
    12061 ttacgcgctg agattttcat ttgcttaaaa aaaacaaaaa aaataaacat attttaatga
    12121 gtagacgagt acttaaacaa aaacacggtt tggagtagtg acaaaaatta tactgcagtc
    12181 cctttttcca aataaacggt gttcaaaagc ttgcatcttc gtaataagcc cagccaaacg
    12241 aaaaatataa ggaactattt ttatttggct gggcttatta cttgatgtag gacatctatt
    12301 ttacaacctc agatcattta ttgcgcaata ctgactaaat gcaactttgt gatatttttg
    12361 tgtacggaca actcctcgat cacatcggca taagtgagcc cttttggaag atcaatcgta
    12421 tagatatttt tgtacactcg gtagtcatca acgaatttta cgtcaaaatt cacatcttcg
    12481 attacgatat ttttctcttc aaaataatcg gtaagaaaat ctttggtttc ttttctatgg
    12541 ataaattgga tttctaactt tttagtcgta ggtacatgga tcacgcgttt cactactgtg
    12601 agtgcaatag caatcccgat aaaactgatg atcgcaatgg aatagtaacc catgccgatg
    12661 gcgatcccta atccagcaat tgcccataaa gaagcggcag tcgtaagtcc cgtcaccgat
    12721 tgtttcgtga cgatgatcgt tcccgcgcct aagaaaccga caccactcac cacttgagca
    12781 atcagccttg cttcatctga acgaatcgtt ccagcaagct gtggatactc taaagcatct
    12841 cttaatgcac cactagcgat ctcaacttgg atcaatgcaa tgatacatgc tcccatacaa
    12901 actaaaatat gcgtacgcat acctgctgga cgatttttat attgtcgttc aaaaccgatt
    12961 gctccgccaa atagcatagc gatccctaaa cgtaaaataa tctctggaat tgtcaatacc
    13021 atatcattca tctaaacacc ctccagtcca atatatcatg tctgaaagga gcagacaaaa
    13081 aaagtgcaat ttttacctct tacgaaaaaa ggactgtgac aaaatttttg tcatagtcct
    13141 ttttatgaat aaacggtgtt caatcagctg accctcggta ttactcaaca aacggcaaaa
    13201 tatggggagc tatttttcct ttgtcttctc taatactcag gtcaaaacgc tgatttcaca
    13261 acctctttag cctactattg aaaattacaa cttacttgct gcttcttcat cgatgatcac
    13321 aacaacattg tcgtgatttt gcaatacact tgctggcaaa tgattcgtta caggtccttc
    13381 aatcatccct ttgattgcat cagctttgct ttcgccaaat gccatcaaga cgatttcttt
    13441 acttttcata attgaaccga ttcccattga aacagctttt gttggtacat cttctgcttt
    13501 atcgaagaat cgtttgtttg cattgattgt tgattctgtt aagtcaacta ctgaagtcgt
    13561 tccgtcaaat gaagcaccag gttcattgaa accgatatgt ccattttgcc cgattcctaa
    13621 gatttggata tcgattggat gtgcatcgat gatttgatca tactttttgc atgctgcttc
    13681 cagatcttct gcttttccat ctggtacaaa cgtttctttg aatggttttt tatcaaataa
    13741 ttggacatcc atgaaatgac ggtagctttg ttcatctgaa ccgcctagtc caacgtactc
    13801 atctaagttt acagaagtca tttcactgaa atccaaatcg cttgaagtca tttctttata
    13861 taaagtaact ggtgtgcttc ctgttgccaa tcctaatact tttgcacctt tgttcatacc
    13921 ttctttgatg atttcaaatg ctttttttcc gccttcttca gcgtttttta cacgaatgat
    13981 ttccatagtg attgctcctt tatattttgg tatagcccaa ttatagcatt ctttttttct
    14041 ttttacaata attagtatag accaaattgc atttttttca ttttcttgac aataaatagg
    14101 aatccgtgtt aggtttcata agatggttca atcaaacaat ccatgttgca tgaaaaccaa
    14161 gcaagtgatt cactgtggac gctttatgtc tgcagttttt ttatgaaaaa ggagttatta
    14221 tgagtatact gacattaaac aacattaccc agcaatttgg cgacaaaatc ctctatgaag
    14281 gtgtcgatct gcaattaaat gctggcgaac atcttgggct gattggacaa aacggagccg
    14341 gtaaaagtac attgattaaa atcatcactg gtgagatctt accagatgac ggacagatcc
    14401 attggcaaaa aaatattcac aaaggttacc tcgatcaata tgtggaagtc gatgaaacat
    14461 taacaatcga agaatttcta aaaactgcgt acgcaaaaga atacgaaaag gaagaaaaaa
    14521 ttgcttttct ttaccaaaca tatagcgaga ccttcgatga ttccttgctg gaaaaagctg
    14581 ggaaactcca aacggaatta gaccaaggag tgttttatca aatcgacacc ttagttgctg
    14641 agatgagcag cgggttgggg atcgatgtct taggtttaga ttcactgttg aaaaatctaa
    14701 gtggtggaca acgttcaaag gtgattttag caaaactact tttagaaaac ccagatgttc
    14761 tgatcttaga tgaaccaacc aatcacttag atgatcagca tatccaatgg ctgacgcagt
    14821 ttcttcagga ttttgacggt gcgtatatcg tgatttccca tgaccaagaa tttcttgatc
    14881 agatcaccac ccatatcgca gacatcgagt ttggaaagat tactaagtat actggccatc
    14941 taaaacaagc attgaaacaa aaggaacaaa atcgcgaaag ctatttgcgc caatatcatg
    15001 ctcaacaaaa acaaatcgaa aagaccgaag cttatattcg aaaatacaaa gccggttccc
    15061 gatcaacgat ggcaaaaagc cgacaaaaac aattagataa aatcgaacgg ctgacgcctc
    15121 cagcttcagc aagtaaagct gtctttgatt ttccattctc gcctattgtc acgacattag
    15181 cagtggaaac aactgattta gtcatcggat atgatcgacc gctacttgca cccatcaatt
    15241 tgaccatccg ctttggtgaa aatattgcca ttcgaggctt caacgggatt ggaaaatcta
    15301 ctttattaaa aacactgatc ggtgagattg aaaaaataag tggcgatttc cacttcccag
    15361 ataacacaaa aatcaattac ttttcacaag atctgtactg ggaaaatcca ttagaaacac
    15421 cactacaata tttaagcagt caatttccaa aagcgactat caaagaatta cgtcgccaac
    15481 tagctaaagc agggttagtt aatcaattag caagtgaacc tctcactaca ttaagtggtg
    15541 gggaacaaac taaggtgaaa cttgcacagt tgacaatgaa ccaaggtaat ttactgattc
    15601 tcgatgaacc gacgaaccat atcgatcaag aaacgaaaga aagcttacaa gacgggatac
    15661 agaaatttcc aggaacagta atcatcgtct ctcatgaaca agaattttat caagatcttg
    15721 tgcaacgagt cattgagatt gaaccaaaat aaacgtttga ttgaatagtt gaaaaaaagg
    15781 agctgtgaca aaagttttgt cacaactcct tcttctgaat aaatggtggc caattagctg
    15841 accttcggta ttagctcaaa aaacggcaaa atatgaggag ctatttttat ttggctattc
    15901 ttattacttg atgcagaaca gcttttcaca acctcttttc gtctattttt tattttcgag
    15961 atttctcttt cctttaaaaa caactgtttg aaagaagaac gcacatcctt gaaaaacaag
    16021 cccgctaacc gttacaccta accagccata ctgctgccac attgtcgtcc ctagcaatga
    16081 accagccgca cctccaataa agtacataaa cataaataca gacccgtggt gctagttaat
    16141 tttaatatcc taaagaatat ctcaaagttc cttcaaaacg tcgttgtgct cgacttaata
    16201 gaacactgta agccagtaat atgctttcaa atctactctc tagcccttta acagaacgtg
    16261 agttgaaatt ttgacaacct aatttttcta aggaagaaaa aacagtctca actgtttttc
    16321 tttgttttcc aagtaagctg tggtctagtt tttctgattt atccatgttt ttacgtggcg
    16381 gaacggatat agtaatgccc ttaagggcaa gcttttcatg gattttatgg ctaatatatc
    16441 ctttgtcccc taggatattg ggaagattcg cttcttccga taaagtctct aacactttca
    16501 cgtcatggac acctggattc gtgattgagt aagtaatcgg aaagccagtc ttagtaacga
    16561 tcatatgaat tttaagacca taaaaataag atttttttgt tgagttatag ccaactttgg
    16621 ctatttgatt caatagtttt gcttgtctat ttctaactgg tttacataat ggactaggaa
    16681 aactatctat aattccgact agttcacctt tggtgagctt cttgatgaaa aagtatcgaa
    16741 taatcttgat agtgtaagct aagtttgaac ttaagcgagt gaatcgactc ctactaggaa
    16801 agtcaccatt aggaaataag acggaacaaa ccgctctata cgtggctctt tggctagtat
    16861 agccattaat tatgccccat ataacacaag
//
LOCUS       DASJTC010000063        16173 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.063, whole genome shotgun sequence.
ACCESSION   DASJTC010000063 DASJTC010000000
VERSION     DASJTC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.063"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            226..894
                     /locus_tag="U4B19_001884"
     CDS             226..894
                     /locus_tag="U4B19_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEN2077615.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1324)
                     /locus_tag="U4B19_001885"
     CDS             complement(1094..1324)
                     /locus_tag="U4B19_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077616.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1451..2833)
                     /locus_tag="U4B19_001886"
     CDS             complement(1451..2833)
                     /locus_tag="U4B19_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN2077617.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2989..4176)
                     /locus_tag="U4B19_001887"
     CDS             complement(2989..4176)
                     /locus_tag="U4B19_001887"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEN2077618.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4183..5214)
                     /locus_tag="U4B19_001888"
     CDS             complement(4183..5214)
                     /locus_tag="U4B19_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEN2077619.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5260..6684)
                     /locus_tag="U4B19_001889"
     CDS             complement(5260..6684)
                     /locus_tag="U4B19_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2077620.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6859..7764
                     /locus_tag="U4B19_001890"
     CDS             6859..7764
                     /locus_tag="U4B19_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEN2077621.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7855..8532
                     /locus_tag="U4B19_001891"
     CDS             7855..8532
                     /locus_tag="U4B19_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEN2077622.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8529..8699
                     /locus_tag="U4B19_001892"
     CDS             8529..8699
                     /locus_tag="U4B19_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077623.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8813..9709
                     /locus_tag="U4B19_001893"
     CDS             8813..9709
                     /locus_tag="U4B19_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2077624.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9764..11134)
                     /locus_tag="U4B19_001894"
     CDS             complement(9764..11134)
                     /locus_tag="U4B19_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2077625.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11372..12118
                     /locus_tag="U4B19_001895"
     CDS             11372..12118
                     /locus_tag="U4B19_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEN2077626.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12115..12954
                     /locus_tag="U4B19_001896"
     CDS             12115..12954
                     /locus_tag="U4B19_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEN2077627.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12955..13904
                     /locus_tag="U4B19_001897"
                     /pseudo
     CDS             12955..13904
                     /locus_tag="U4B19_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14045..14797
                     /locus_tag="U4B19_001898"
     CDS             14045..14797
                     /locus_tag="U4B19_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077628.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14816..15967
                     /gene="queG"
                     /locus_tag="U4B19_001899"
     CDS             14816..15967
                     /gene="queG"
                     /locus_tag="U4B19_001899"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEN2077629.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5251 a         3013 c         3245 g         4664 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttttcttg aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt
     1381 gactagttct tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata
     1441 ctcgttatag tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac
     1501 tcataccacc tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca
     1561 agagactatg cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa
     1621 caaaatcaac tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta
     1681 aacctgtcgc gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt
     1741 ggtaatgctg gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg
     1801 cttcgatttt catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag
     1861 agtatcctac ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc
     1921 cgacttggtc ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc
     1981 tatctaggat ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa
     2041 aacttcgatc gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc
     2101 ttttcaagtg ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt
     2161 tcagacgttt tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc
     2221 ataacttttc tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg
     2281 agccattgtc taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt
     2341 agattccggt ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg
     2401 ttttaccaaa gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga
     2461 catgatggtt cgcttcgtct gcatattttt tatagataga attgatttct ctgttctttt
     2521 ccatataata ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa
     2581 gatctctggc acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag
     2641 ccaagattaa tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta
     2701 atttagttgt aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt
     2761 tgcagtcaat acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt
     2821 gattcgccca cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg
     2881 tcaataaaaa acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag
     2941 aggttatttt tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac
     3001 agcttctgga tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc
     3061 atagccgaca actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc
     3121 ttctttgaca agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa
     3181 gacaccttct tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc
     3241 ataatcatag cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt
     3301 cccagcttct aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg
     3361 agctctgcgt atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc
     3421 atctaagtct cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc
     3481 gcattcgcta cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt
     3541 catgatgacg attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt
     3601 tgtatctaat aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat
     3661 acctgaattg acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa
     3721 gcgatctgtt tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga
     3781 agcgctactg gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc
     3841 atgatcgatt gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc
     3901 ccagtttgct tttttatcat attctagctc atctaaagta aaactaatga caccgatatc
     3961 gtcaaagttt gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg
     4021 cgttccataa gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc
     4081 aatcaaattg atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc
     4141 ggcaaatttt gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt
     4201 gattttgaat tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa
     4261 tgctgagaag cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg
     4321 tgccagtttt cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac
     4381 cacactagaa gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg
     4441 agcggcttct ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac
     4501 aagatttttt tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa
     4561 atcgaaagca cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa
     4621 aggagcataa gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc
     4681 tcccgtcaag ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt
     4741 gccgcgagag gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc
     4801 cattgtacct ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc
     4861 ttcattttcg ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc
     4921 accatgcaga ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag
     4981 agaaaagcgg ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg
     5041 atctttttcg atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga
     5101 ttttaaactg acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt
     5161 ctccaaggtt atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa
     5221 gatcctttct taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc
     5281 tggagccaat ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt
     5341 aatcttccaa gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc
     5401 ataagctttg aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt
     5461 catcccagaa gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt
     5521 atctagtaaa gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg
     5581 accatattca acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg
     5641 gaagtagaac aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa
     5701 gaaaaagtaa gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat
     5761 gacgcgtgta agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag
     5821 accaacgatc caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac
     5881 ggcagttgtt gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa
     5941 ctgatcattt tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg
     6001 acggatcgca gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga
     6061 aataacagca gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg
     6121 agtaacaaga taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc
     6181 acctagagaa gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt
     6241 actcaaagct ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa
     6301 taagatgaaa acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc
     6361 aaagaaattg gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa
     6421 ccatggtttg aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg
     6481 atcaaaaata atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat
     6541 ttttgcattc gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa
     6601 ataagtgctt aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac
     6661 acgttggatc attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta
     6721 agtaaaccgg ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc
     6781 gcttcctttt taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag
     6841 gagtaaggag ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta
     6901 gaaaaagaat tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt
     6961 ggtcctgcta ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat
     7021 catgtgaaaa atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga
     7081 gactcgacgt tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt
     7141 ggtgggagtg ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg
     7201 atggtctctt ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg
     7261 gaagaaacct tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc
     7321 cttttaaaag atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct
     7381 gttgaaacag tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc
     7441 gcccagcatt tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt
     7501 accagtatca aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct
     7561 ctgacggatg aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta
     7621 tttagccgaa tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg
     7681 aaaaacgaaa attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa
     7741 accgcagttt cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa
     7801 acttgtggcg gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt
     7861 acacttatca tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa
     7921 gaagcttctg ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga
     7981 aaaatagata tagagactga actacagctc ttggcccagc atgatcggat cattttccag
     8041 tttccgtttt actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg
     8101 gaacgagcag cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg
     8161 gcggaaaaag aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg
     8221 ccgtatcagc gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca
     8281 caatttatgt atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat
     8341 ctctcattgg aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta
     8401 caggaaaatc agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca
     8461 ctgactgagg caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga
     8521 acttcattat gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag
     8581 aatatcaaga ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac
     8641 gaacaatgca gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa
     8701 attttcttat aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag
     8761 aatatgaagc gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga
     8821 tgaaaatgag tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc
     8881 tcgtaagcgg atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg
     8941 agttgcgggt ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac
     9001 ggaaacaacg acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa
     9061 agagacacaa acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat
     9121 cgtaaatgat tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca
     9181 gacagaaatt cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa
     9241 agcacgtgag cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca
     9301 ggcagcagaa aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa
     9361 ttatacgaat catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca
     9421 agcagagtca acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt
     9481 agaagcttct gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa
     9541 taccaacagc gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa
     9601 tcaaatagcc gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag
     9661 catggagaat tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata
     9721 aaaaagaacg aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt
     9781 tttcgcgtac aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg
     9841 agattctcgt ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg
     9901 tcacataatt cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg
     9961 cgtgcttctt tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag
    10021 gcaaaatact tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg
    10081 gtagaagata atttaagatc agaccaatta agattccacc tgagattgct acgacaggat
    10141 aaaaaggcgc accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg
    10201 caagtgtata gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg
    10261 ctgtaatcac gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag
    10321 tttgtacaaa aggtgaacca atctcactca attgattcca cggatagata ctcacaatga
    10381 taaaaatagc accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa
    10441 tgatcgtctt tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt
    10501 gataggaagc aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga
    10561 atccaccatt tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa
    10621 ataagattac cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg
    10681 aaaaccaaaa ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa
    10741 acaagacgac aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat
    10801 aagatcctac agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc
    10861 aagcggtcaa gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac
    10921 cagtcgaagg atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga
    10981 atcctgcaat cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg
    11041 cacccataaa cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg
    11101 cactaagact tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc
    11161 aacaagaaaa aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt
    11221 gaactcttga tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa
    11281 ctttttcatc attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa
    11341 agttaggtaa acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga
    11401 cctttcagcc gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc
    11461 acaaaaaatt accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc
    11521 tttgctagag ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa
    11581 ctcagtccgt aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc
    11641 aattgatgta ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag
    11701 acctgggaaa aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga
    11761 atatgccaaa agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc
    11821 aagagctcta gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga
    11881 tgcgttaagt gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac
    11941 gattttgatc gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct
    12001 tttaaataaa cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct
    12061 tcaattggcc tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc
    12121 caatccttta ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc
    12181 atgcttgtag gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct
    12241 ttgatgggag acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt
    12301 ggttttagtt acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt
    12361 ttcgtgactc agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc
    12421 ttttttgcat tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat
    12481 attttatttg ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt
    12541 agtggaatcg tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc
    12601 gatccgacaa tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg
    12661 ttctttttga cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt
    12721 gccatgttga tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg
    12781 atcattacag ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat
    12841 agttacaatt ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt
    12901 gcttttttat tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa
    12961 acaaaaaaat cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga
    13021 aagcaggctt ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc
    13081 aattcgatct tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt
    13141 atcgtaccag tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag
    13201 gcaagcgatg cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg
    13261 ttcgataact tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc
    13321 aaaaatgtag aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat
    13381 gcttggctag acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct
    13441 gaaaaagatg cagaaaatgc gacactctat aaaaaaatgc agaagcatat gtggaaaaac
    13501 taaaagaatt agatacacaa gcgaaggaaa gttttgcttc tatcgaagag aacaaaaaat
    13561 tattagtaac aagtgaaggg gcattcaagt atttttcacg agcatatgat ttgccagcag
    13621 cttatatatg ggagatcaat acagaaagtc aaggtacgcc tgatcaaatg aaagcgatca
    13681 ttgatcagat aagagcaaaa gaagtaccgg ttttattcgt ggaaaccagt gtagactcaa
    13741 gaagcatgga acgggtagca aaagaaacgg gtttgaaaat ctatgataaa ctgttcactg
    13801 attccatagc aaaagaagga gaacaaggag attcttacta tcagatgatg aaatggaata
    13861 ttgaaacgat ccatgaaggt ttgtcacaaa caaaagaaag ctgaaaagtg caaggtctag
    13921 cttgaggagc agaccttgca ttttttataa gagatatgaa aaaaaattat gctgtttttc
    13981 gtcgtacaga tacctttttt gaagaaattc cgttatagtt agagtaagga aggaaggtgt
    14041 ggctatgaat tttacacgta aagataaata tacgattaaa gaagaagtct ttcgattgat
    14101 cagccagcag gaaaataccg acaaggatca agaccttcaa tttcttgaag cagaattagc
    14161 taaattattc atagcaaatc aaaatgagac aacagaacca gatgaagagc tgtacatagg
    14221 caaatggttg tttgatgaat ttcagccttt agatgaaaat accggtatcg gttattttgt
    14281 gttcctttct ccagtcggct tcacacctgg cgtattagtt aaacaaaacg acgaaataac
    14341 cgtcaaagca tggcctgcat tattttcagt catcaaagca gaaggagtaa tcaaaaagat
    14401 ccagtcggcc tattataaaa gtcaatttat tgccaacagc aaattaatga atatgtttta
    14461 taatcgtcta gtagaaattg ctaggcctgg gaaaatcccg caaacattca cgtacacaca
    14521 gcaaaaaaat ctctatgaag aagtattgta ttcccgcgat ctgtctgagt tttcgccatg
    14581 ggaagaggta aggctccatg aactgttaag tacaaaacca gtttcattga atttttttaa
    14641 tgcattagat aacaaaagaa aattaatttc aacaaattat tgggaagtat ttgctgcaag
    14701 aaaaaaatgg atggaagaaa agaagattgc cgatcagcag atccagtttg tccagctgaa
    14761 aaaagaaaaa ctatattatg ggttatacag ccgttaacag caagcgagga agaccatgac
    14821 attaaaagaa gaaatcatac aagaaagcaa acgattagga attgacaaaa tcgggttcac
    14881 cacggcggaa ccttttgatt cattaaaaga atcgttggaa gaacaaaaag cagctggcca
    14941 tacttctggc tttgaacatc caaatatcga tgagcgtctt tatccagaaa tgacatttga
    15001 tcagccgaaa tcgatcattt ccattgcact tgcgtatcct actaggatcc atgaagaagt
    15061 gcctagggat gaaaaacgcg ggcagtttgc tcgtgcttct tgggggatcg attaccatga
    15121 tatactcaga gaccgtttga accgattgat cgaattcatc caaacaagag cagaaaaatg
    15181 gcaaaaagaa gaggaatggc gtctagctcc gcaagtagat actggagaac tgatcgatgt
    15241 cgctgtagca cagcgagctg gtttaggatt tattggacga aacggattgt tgatcactga
    15301 agagttcggt tcatttgtct atcttggtga gatcgttact aatattgtat ttgagcctga
    15361 taaaccagga gaatttggtt gcggtgactg tatgcgctgc gtgactgctt gtccgacaaa
    15421 agcattgctt ggtgacggaa gaatgaatgc ccagcgatgt ttgtcttatc aaactcagac
    15481 aaaagggatg atgccggaag aatatcgtaa aaaaatgcga aatgtcattt atggatgtga
    15541 tatctgccag ttagtctgcc cttacaatca aggaaaagat tttcattttc atgaagagat
    15601 ggaaccaaaa gtagacgaaa tatatccaaa attaaagcca atgctttcga tgtctaataa
    15661 agacttcaaa cagcaatttg ggcatttagc tggttcatgg cgtgggaaga aacctttgca
    15721 gagaaacgca ttgattgctt tagctaatct aggtgatcga acggcattgc ctgagatttc
    15781 agaatgttta acagatgtcc gccctgtcat cagaggaaca gcagcttggg ctattggacg
    15841 tctaggaaca aaagaaccgg aaaaatggct tgatgtatta gatgaaatat tagctaaaga
    15901 aacagaagaa gtcgttataa acgaattgga acatgcccag aaattattgc ggagaaagtt
    15961 aaaataaaaa aatagttaac attattcaaa gtttttgtcc agtaattgtt gtaacaatta
    16021 ctggtttttt tgtatttttt acattttttt aagagaattt gataaaaatc aaaaattttt
    16081 ttgctttttt ttatggatta ttgcagtgaa tgttacagaa tctgcgtttt tcattcaaaa
    16141 ataaaaaaac atagaaaaac gattactttg ctt
//
LOCUS       DASJTC010000064        15346 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.064, whole genome shotgun sequence.
ACCESSION   DASJTC010000064 DASJTC010000000
VERSION     DASJTC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.064"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            129..620
                     /locus_tag="U4B19_001900"
     CDS             129..620
                     /locus_tag="U4B19_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEN2077630.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(644..3019)
                     /locus_tag="U4B19_001901"
     CDS             complement(644..3019)
                     /locus_tag="U4B19_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEN2077631.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            3345..3971
                     /locus_tag="U4B19_001902"
     CDS             3345..3971
                     /locus_tag="U4B19_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEN2077632.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            3994..4698
                     /locus_tag="U4B19_001903"
     CDS             3994..4698
                     /locus_tag="U4B19_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2077633.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            4890..5723
                     /locus_tag="U4B19_001904"
     CDS             4890..5723
                     /locus_tag="U4B19_001904"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEN2077634.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            5920..7848
                     /locus_tag="U4B19_001905"
     CDS             5920..7848
                     /locus_tag="U4B19_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEN2077635.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            7986..8441
                     /locus_tag="U4B19_001906"
     CDS             7986..8441
                     /locus_tag="U4B19_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEN2077636.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            8911..9180
                     /locus_tag="U4B19_001907"
     CDS             8911..9180
                     /locus_tag="U4B19_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077637.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            9580..9834
                     /locus_tag="U4B19_001908"
     CDS             9580..9834
                     /locus_tag="U4B19_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEN2077638.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(9923..10969)
                     /gene="holA"
                     /locus_tag="U4B19_001909"
     CDS             complement(9923..10969)
                     /gene="holA"
                     /locus_tag="U4B19_001909"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEN2077639.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(11007..13280)
                     /locus_tag="U4B19_001910"
     CDS             complement(11007..13280)
                     /locus_tag="U4B19_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEN2077640.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(13273..13812)
                     /locus_tag="U4B19_001911"
     CDS             complement(13273..13812)
                     /locus_tag="U4B19_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEN2077641.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(13948..14166)
                     /locus_tag="U4B19_001912"
     CDS             complement(13948..14166)
                     /locus_tag="U4B19_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEN2077642.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(14163..14408)
                     /locus_tag="U4B19_001913"
     CDS             complement(14163..14408)
                     /locus_tag="U4B19_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEN2077643.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(14524..15252)
                     /locus_tag="U4B19_001914"
     CDS             complement(14524..15252)
                     /locus_tag="U4B19_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEN2077644.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         4695 a         2967 c         2733 g         4951 t
ORIGIN      
        1 ttatctatta aaataaaata cttcgaactc aaatgagaag aattttagca aacaggtaag
       61 aatctgccct aatcctatga taagatagat gtatggtaaa tatttatatg tatatcagga
      121 gggttcctat ggcaaaagat gctagcttcg acattgtatc agaagtcaat acagaagaag
      181 taaaaaatgc gattcagcaa acaacaaaag aattgtccaa tcgctttgac ttcaaggatt
      241 ctacagtaga aatcaaatta gagaacagca atctagttgt attgagcgat gatgatttca
      301 aaatcgaaca gatcaaagat gtattatttg gtaagctgat caaaagaaat gtgccaatca
      361 aaaatatcca tttttccgaa tcagaacacg ctctaggtgg caaagctcga caaactgccg
      421 atttggtcaa cggaatcgat cgtgaaaacg cgaagaaaat cactacagct atcaaaaatg
      481 aaaaactgaa agtaaaagca caaatccaag aagatcagat tagagtgaca ggtaagaacc
      541 gtgatgatct acaagctgtg attgctttat tacgtaaact tgacttacca atcgagttgc
      601 aattcactaa ctatcgttaa tcaaaaaacg aggctgacgt ctgtcattcc tcgttttttt
      661 gatttactgc ttcttttgat aaccaaaaac gctgtattcc ttcttccaat tccccaacac
      721 ttcgtacttc tcttgctgtt tgccaatgtt gtgggaataa acgtcgataa gaaaaactag
      781 aaaatcgttg atctgccaat acaacgattc cttgatccgc catgccacga atcactcgac
      841 caccagcttg caagactttg ttcattccag gaagctgata agcgtaagca aatccaaggt
      901 gttccttctc atcataatat gatttgatca gttcctgctc atgattcatc tgcggcaaac
      961 ctacacctac gatcatactg ccgatcaatc ggtcccctct caaatcgatt ccttctgaaa
     1021 aaatcccgcc aagtacgcaa aatccaatca atgtttcagc gggatttgtt tgaaattttg
     1081 ttaaaaatcg ttcgcgttct tcctcattca tgtccgtttc ttggattagc acattgctgt
     1141 cgggatattg ctgtctgaat gcttccaccg caagatctaa atattgataa gaaggaaaga
     1201 aaacgaaata attcccgacc tttccatcag caaaagcatg aatcgtctct actaattttg
     1261 ccagactgtg ttcccttttt cgataagtcg tctctaggta atttgcgatg aaaacttgct
     1321 ggtttccttc tgggaaagga ctcggcagtt tgtaagccag acttttttgt ccccccagtg
     1381 tttcctgata ataagagagt ggtgaaaaac tagcagaaaa aagaatactg ctttttcctt
     1441 tatccaacga ctgctcaaga aacaagcttg gatcaataca aaactctttt actatcagat
     1501 cgcgatacgt tttttcgact gttgtttcgt agtgatcatc ataaaattca ctgaccttca
     1561 aaaaatgaag caaatcaaaa taataaggaa gcaattgttc ttgtgatggg tgctcaggaa
     1621 attctgccag ccattcttta attttttcgc ttaattgata acctgccttt actaaagatt
     1681 cagccggtgc tttttgatga tgataatccc aatgatcttc ttttgctatt tctcggatcg
     1741 aatcaaattc ttttaatagt ttgttgaaac ggcgatgaag ttttttgaat tcctttggta
     1801 tagcttcctt acttcgtttc gttttttcat aagatagttc tgccgaatac atctctcggg
     1861 aacgattcac caaattatgt gcttcgtcaa tcaaaaacag atagtcctcg tttttttcat
     1921 cttcaaaaaa acgtcttaaa taaacagttg gatcaaataa ataattgtaa tccccaatga
     1981 ttacgtcaca ccagatactg acatctaatg atagttcgaa aggacaaagt gtatgctttc
     2041 ttgcataagt ttcgatgact tcacgagtga tctgattctc atgattcaaa agatcccata
     2101 aaccttcatt aatccgattg taataaccat tggcatatgg gcactgatct ggattacagg
     2161 tcgtctcatc taaaaagcag atcttgtctt tcgccgtcaa agtgacactt tttacttcag
     2221 ctcctgtatc ttttaatgct gtcatagcat cttcagctac ttgtctagtt attgttttag
     2281 ccgttagata aaagatcttt tctccctctt cttcccctac agctttcaaa gcggggaata
     2341 acgtagagat tgtttttcct gttccggtag gtgcttctac aaacaatttc tgtttcgtac
     2401 ggatcgtttt ataagcagct accgctagtt cacgctgacc tttcctatac tcatcatggg
     2461 ggaatttcaa agccatcaaa gaggcattgc gtacagtgcg ccagttttct tgaaaaacaa
     2521 gccattcttt ataatcttca gtcagctttt taaaaaaagt ttctaactgc tctcttgtct
     2581 gatgttcaac ctttctcgtg atcacttctt ctgccgtctg gaagtaagtc aattgcagat
     2641 ttatttcttg taaatcatgt tcttgactat agatataggc atacaccctt gcttgatgaa
     2701 agaaaagttc cacttgttct ggttctaaat cttcaaatcg tggttcggag gttttgattt
     2761 catctatata ataaataccg tctcttttga aaataccgtc tgcacggcct tcaacgatca
     2821 ggtaatattc atctaattga actgttgttt ttaaaaagac ttctttttca tagttttctc
     2881 ccgcatcttt ttggagcttg cggtggattc tcgctccttc taatgcagta tggttacttt
     2941 ttttacgatt gtcgatactt ccacgtcgca aaataaattc tactaagtgc cgcacagata
     3001 tttttgactg tttgatcatt tccaccacct ctttctctct aagattacct gaaaaaacct
     3061 agtttagcaa aaagagctct ctttccaaac ataaaaaaac accctgctga ttaggcaggg
     3121 atataggata tagaatttgg atttttggat gaatctaatg taacaaggac atattaaaat
     3181 gctatgaaaa cttactttcc ttctctttag aatatccaaa tacttttcat atagtctgat
     3241 aattcagata agtaaagaga aataagtacc tgtttttgtt tcctagcaat gaatgcgtta
     3301 tactaatgga tgaaagaagt tttggctatc aaggaggaat gcacatgcat atcgaaagat
     3361 taaaaacagg aatgattgaa gaaaactgtt atttggtcta caacgaagaa gcacttttga
     3421 tcattgaccc tggagaagat gcggaacgaa tcaaacaaga aatcaaaaaa acaaaccaac
     3481 agccggtagc gattttgctt acccatactc actacgatca tatcggtgca gtggaacctt
     3541 tacgcgcgta ttatcaaatc ccagtttatg tcagcccact agagcaaaaa tggctaggtg
     3601 atccgatttt gaatctttct ggactcggta gacatgatga tattgctgat gttattgtct
     3661 cgcctgcaga atatgaattt gaaatgaaaa cttatacttt aggaaacatg tcttttcgcg
     3721 tcgtccctac cccagggcat tctataggaa gcgtcagctt tatttttgat gattttgtcg
     3781 tttctggcga cgccctgttt agaggaagta tcggtagaac cgatctgtat acagggaatt
     3841 tagaacaatt acttcatagt attcggaccc agctgtttgt attacctgat gaatttgcag
     3901 tctatccagg acatggcgat gcaacgacga ttgaacagga aaaaaggaca aatccttttt
     3961 tcaatcgtta aaacgactta ggaggagaat aaaatgacag aaactacact ttatatcgta
     4021 cgacacggaa aaacgatgtt caatacaatt gaacgagtac aaggctggtg tgatacaccc
     4081 ctcacaaagc aaggacaaga aggcattcat tatttaggtc gcgggttaaa aaatgtcgac
     4141 ttttcatttg cttattctag tgacagtggt cgtgcaatcg aaactgcccg tattattcta
     4201 ggagaacatt ctaagggaaa agaaattcct tattttattg aaaaaaggat tagagaatgg
     4261 tgtttcgggt ctcttgaagg tggatatgac atggaaatgt ggggtgtgat ccctcgtgta
     4321 ttgaatttta aaacatatga tgaaatgttt accacagaag tttcttttga acaaattgct
     4381 aatgctgtat acaacttaga taccgctggg tgggcggaaa cttatgaaac attgcgagac
     4441 cgtgtatgga caggatttga agatatcgcc catcactgcg aaaaaaatgg tggtggaaat
     4501 gttttagtcg tatctcatgg tttgacgatc gcctttttgc ttaatctgat cgatcctaaa
     4561 cagcctgttc gtgctgggct tttaaatggc agcgtaacaa aggtcactta tgcaaacggt
     4621 cgattttcta tccacggcat caatgatacg tcttatatcg ataacgggaa aacacatagt
     4681 cgcgactcgt ttctataatg gggatatttt aatttttacc agagaatcat aaaaggcaaa
     4741 gtctgaacga gggctttgtc ttcttttttc tcttcgtgta cttatctgct ttatttctca
     4801 agtttttctt aatattaaaa aacagattgg gtttctcaaa aaataacggt atactataag
     4861 atgtatctta attgggaagg aaggaattta tgttttttgc aaatattatc aaagcagtga
     4921 ttctcggtat tattgaagga ataacggaat ggctgcccat cagtagtact ggtcatttga
     4981 tcctagcaga tgaattcatc aaactggatg ccagttcaca atttatgtcg atgttcaatg
     5041 tcgttatcca attaggggcg atcttggctg tcgtcgttct ttattttcac aagttaaacc
     5101 cattttctcc aagtaaatca acggtagaaa aaaaagatac gtggacactt tggtttaaag
     5161 tgatcgttgc ttgcatacca gccatcatca ttggtttgcc tttggatgat tggttagaag
     5221 caaatttcca caagttctta ccagttgcca ttgttttgat cgtttacggt attgccttta
     5281 ttattgtcga aaaacgcaac aaaaacaaaa cacctcgttg gacttctctg aatgacttta
     5341 cgtaccaagc agctcttctt gttgggttat tccaagtctt ggcacttgtt cccggaacat
     5401 cccgttctgg cgcaacgatc ctaggatcta tcttgattgg tgcttcgcgt tttgtcgcta
     5461 cagagttttc atttttctta gggattcctg tcatgtttgg cgcaagcggc ttgaagatcg
     5521 tcaaatattt agctgaaggg aattcattca aaatggaaga aactgttgtt cttcttgtag
     5581 gaacattcgt atcttttgtg gtttccgtct tagctatcaa attcttacta agctatttga
     5641 agaaaaacga ctttaccgct tttggctggt atcgtattat ccttggtatc attttgattg
     5701 gttattggtt tattgcaatg tagaaaaaac cagaacactt ctcttatttg agaaatgttc
     5761 tggtttttta ttttctttaa taataatcat tataaataat aaaaacaaca tcaagcttaa
     5821 tcaccaaatg ataaccatta tcgttctcat ttagctgtta ttattatcaa ttacattttt
     5881 attatgctat ccttgttttg acaaggaggt ctaataacga tgactaatca gaaaaaagct
     5941 tttgcagcaa caatcggctg ccttttattt atgatcttcg gttttatctt cgaccgattc
     6001 tctcttacca tcaccccact tttttatatc agtgccatta tcatcggtgg atataaacaa
     6061 acatcggaag gcttgaaaga attgtggaat gatcggacac tgaatgtgga tttattgatg
     6121 gctttagctg ctattggtgc ctgtttgatc ggtaattact ttgaaggagc aatgttgaca
     6181 ttcatctttt gtttaagcgg tgcattagag gaatacacaa ctaataaaag tcaaaaagaa
     6241 attactgctt tgatgaatct tcaaccacaa aaagcccaat tattaaaaga aaatggacaa
     6301 atgcaagaag tagatgtttc tcaactaaag gtggataact tggtatttgt cgctaaagga
     6361 gcagctgttc cgatagatgg ctttttagaa agcacacagg caacgatcga tgagtctgca
     6421 ttaagcggtg aatctgtgcc tgtagaaaaa tatttaggcg atgagatgta tgcaggaacg
     6481 ctgaatcaag gaaatcccat cacgattcga gtaacaaaaa cgagttccga aactgtcttt
     6541 gctaagatta tccaattagt agaagaagcg cagaatacgc caactcaaac agcttcattt
     6601 atcgaacgaa tcgaaaataa ttatgtaaaa ttgattttgc ttgctgttcc tctaatgatc
     6661 cttctccccc actttttcct aggttggagt tgggatgaaa gcttttatcg aggaatggta
     6721 ttattagttg tggcttctcc ttgtgcgctt gtcgcatctg ctacaccagc ttctctagct
     6781 gctctttcaa attctgcaaa aaatgggatt ttaatcaaag gcggtatcca tttagaaaag
     6841 ctttctgaat taaaagcgat tgcctttgat aaaacaggaa cgctcactaa aggaaaacca
     6901 gttgtaactg acagcttttt ctttgatgaa aaggatttgg cacaacaact tcttgttgct
     6961 atggaacaaa aaacgacgca tccacttgct catgcgattt tgcaatattt cgattgcaca
     7021 atacctgaag aaatccaata cttaccaata gaagaaatta ctggttttgg attacaaact
     7081 acctatttag gtgcgcaatg gaaagttggc aagcacgcat atgatcctga gacgatgata
     7141 atcagcaaag agattgcgga aatgatcgaa cgtttggaaa atcaaggcaa aaccgtgatt
     7201 tatctttcta aagatcagca gttaattgct gttttaggtc tgctagatat tccaaaagca
     7261 aatacccaac aagtaatttc ctactttaaa tcccaaaaca tccatacaag tatgataacc
     7321 ggcgaccatt ctggcacagc aaaggccatt gctgaacaag tagggattga acattactat
     7381 gcaagctgta ctccggaaga aaaaacacaa ctagtcaaaa aagagaatga actttaccaa
     7441 gtaaatgcga tggttggtga tggagtcaat gatgcgcctg ctttagcagc cgcttcttta
     7501 ggtgtcgcta tggggcaagg gacagatgcc gctatggata tcgcagacat cgtcttaatg
     7561 aaaagcgact taaataaatt tgtctacagt catcggctat ctttaaaaat gcggcggatc
     7621 atcaaacaaa atattatttt ttcgattctt gttattctct tgttgattgc ttctaacttc
     7681 ctgcaatttt taaatctacc tcttggagtt gtaggacacg aaggaagcac aatattggtt
     7741 attctgaatg gcttgcgttt actaagacct ttacctgatc tttctgattc ttcaaaaaaa
     7801 tgtaagtctt gtccactata caaagcgcgg ctgtcttctg tcaaataagc aaagtgtttt
     7861 tttatgattt tgtttatttt atgaaaaaaa cagcttatga ataggtcttg aaggatgaat
     7921 tcctactttt gtaactttct aaaaacatgt tatgattgtt tcgaagaaag tgaggaatat
     7981 atttcatgga aagttggctt tttttaggaa ttattttact tgtagcaatt ttagctaaaa
     8041 atcaaagtct gatgatagcg gcagcttttg ttttgattat gaagctcata ccagcctccc
     8101 gttccttttt cccttggctt caaggcaaag gaatcaatat tggggtgacg atcatcacag
     8161 cggctattct aatccctatc gcaacaggag aaatcggtct acgtgatcta ttagaagcct
     8221 ttaaatcccc tattggctgg gtagctgttt tttgcggtgg gctagtggct gtcttatctt
     8281 ccaagggtgt aggattgatt gcttctgatc cacaaatcac tgttgcactt gttttcggca
     8341 cgattttagg tgttgtcttc ttaagaggaa tcgccgccgg accggtcatt gcttctggta
     8401 tcacttactg tatcttacaa gtatttgctt taggaaaata gaacaaattt tgtctatttt
     8461 aggagattct taacaccccc aatttactga tccgtgatat actgacaacg aggtgataca
     8521 atgttcgaaa aattaaagta tttcatagga acaatcggct ggtttcttct agcggtactt
     8581 ttgattctgg gcttagctta tctattattt cctgatttga tacaaaatgt gtttgatact
     8641 gctttttgat tgacctatta aaacgatata ggggatgctc ctgcgatgca ggggcttttt
     8701 ttaatcaatg ttttgtaata atttaggccg tgccaatgag acttcctata gaattcgatg
     8761 ctcttttttc tttttggctc ttctgctctt ttttgttata ctagattaca gtggacaaaa
     8821 gaaagaatga tcgcatccac ttcaacgaat atcaaaatgg aagaattagt gcctgttttc
     8881 aggttacttt gaaaagaaag gaagacgctt gtggaaactt ttgaaggtct tttaattgat
     8941 acgtacgttt tacaagaaca gccaatgatg ctgactggga tcaacttgca gcttgaggac
     9001 cgtgtgatca atggcctgct taaagatcgc tatttggcaa aatatatctt acgtttgccc
     9061 ctaaataaat atgtgctgga agtaagtggc aaatggaacc gtcggaacca gttggtcata
     9121 aaaaaaatgt tgataaaaaa tatcgatgaa tacattaaaa taatcggtac accagaatag
     9181 cctttactga tcaaaggata ctggttataa aataactgca gtttcttaat agaaaaacaa
     9241 agaacaacag ggaatcgtat cccttttcaa aaaaaacttt gcaaacggtt ctctttgtta
     9301 tataataact tagtaccaaa cattcccatt caaactttgt ttaaatagat aaataaaaat
     9361 ctagtttggt ttgacgctat tgcctcccac aatagcgtct ttttgatttt tttcagcttg
     9421 tactatccgt ttttactgaa aaaaaatcct aataaaagac tgttaaggaa aaaaacttgt
     9481 catgttcata ttgcaaagca cgctgccttt aggtataata atgaatgttg aattatgcgg
     9541 taacacgcgt aagaacttaa gttatcggag gtgaaactaa tgccaaacat tgaatctgca
     9601 atcaaacgtg ttcgtacaag cgaaaacgct aatgtgaaaa attcatctca aacaagcgct
     9661 atgcgtactg caatcaaaaa atttgaagat gctgtagctt caggtgctga caacgtagat
     9721 gcgttatata aagaagctgt taaagctatt gatatggctg aatcaaaagg tctaatccac
     9781 aaaaacaaag ctaaccgcga caaatctcgt ttaagcaaaa aaatcgctaa ataaaagaat
     9841 cggcttcggc cgattttttt atttgtcaaa aaaaggacaa aaacaaacga ccttcccgca
     9901 tgctttgccc tcgtctttct tcttatcgat ttcttaacaa ctgccctgtg cgcaaaacga
     9961 atagttgaaa acttaattct ttttcgattt gtcccgtttt gatttgataa tccatctcaa
    10021 ctaattgatc aaacaactgt accagcaaat gttcatcgaa tttttttact tcctgcatgg
    10081 caagttttac acgatatgga tggattttca atgtttcagc aatatttgcc tgctgataac
    10141 caatctttac aagaaatttt gtctgtaaaa ataaacgtaa ttgagaaagc aaaatcgcat
    10201 taattttgat tgtttcttcc ccttgcataa ctaaatcttc gtataaacgc aaagcttgtt
    10261 ccgtcttccc ttttaaaata tattgggtca tgtcaaaaat attatgttct aacgttttag
    10321 gaactaattc ttctacttct cttaccgtga tttgctgttg attttgtcca taaaggacca
    10381 atttctgtaa ctctctcatc attttggata aatctaagtc ggtcaacctc aagaataaat
    10441 caattgcact tcgttccatt ttgattgagg aattctctaa ggtattcatc agatattgac
    10501 gtacttcttt ttcattcaac tgttggacat tgatgataat tgctgttttt ttcaattgtt
    10561 tcgttacttt tttccgttca tccaactttt catatggcgc aaagaatact aatacagtcg
    10621 attctaatgg ttctttcaaa taagccgtca ataaatcagt attttgttcg attccattgt
    10681 ttactttttc acctgttaaa aaataaggat tctctacgaa tactaaccgt tgatctccga
    10741 aaaacggcaa cgtctctgct tcagctacta cctcatctaa acttgcattt tccatgtcaa
    10801 aacttaaaaa atttaagtca tcttcgccct ctaattggat ttttttgaca atttctgtcc
    10861 gaacttgatc ttggagaaac ttttctgttc ctaaaatcaa gtaacaagat gcaagttttt
    10921 ctttacgtat tttttgtaac tctgcttgta tactcaaatt actcaccact ttcaaaaatt
    10981 atcctaccat gagcagatga gaattactag tctatcattg tttccactag cccatgagga
    11041 tgaaaaactg gatgccagcg ataggtgatc attccttgtt gatccgttcg ccatgttttt
    11101 atctgatttt cttctagtgt ctccaatact tcgctgtgcg ggtgaccata acgattattt
    11161 acgccgcagg atataatccc atgttttgct tcaatatggg aaagaaaatc tgtgttactt
    11221 gacgtacggc taccgtgatg acctaatttg actacatcgg ctttcagatt aggataaatc
    11281 aaaagcaatt gtttttccat tgcttgattc aaatctccta aaaataaaaa gcgcctattg
    11341 ttatattcca tccatagacc aatcgaatct tcatttcttc cctctccctt ttcaaaagga
    11401 gtcaatacat aaagatcata ataaccgctg atcctgtccc catgtttgat ttcaatgatc
    11461 tcagtaccat ttttttgagc ttcagataaa tgcctttgta atggctcatt tcgtccagcg
    11521 ccccaaccga tataaatttt tttgatagta aaatgctgag ataacgtggg taattctccc
    11581 acatgatccg tatcgtcgtg tgtcaagatg agtttatcga ttacacttat cccctgacct
    11641 tttagatatg gaacgatatt tctttcagca ggaggacgta tcataccttg cgcccacttt
    11701 tctctttcaa agtgtaactg acctccagta tcgatcaaaa tcgtttcttt tcgaaaagga
    11761 ctttgcaaaa agatactgtc tccttgaccg acatcgataa aagcgatcgt tgaagaaaag
    11821 cgtaaaaatg gcaaagtcaa aaaaataaaa gaaaaaagac caacataaaa caagtgtttc
    11881 acaggttgtt ctttcaacct atccatacaa atcagtaatc cgaaaatata catcccagat
    11941 agcacaaggg gcaaacgtcc tataatgaga gacgaccctg aagaaaaatc gattattcgc
    12001 tcaaataaga ttaatccctc ttctatcagc aaaagtaaag tttccggcag ccagcgattg
    12061 aagagtgtga gaaataacac accaggaaga aaaagaaact tgaataccgg tgcaatcagt
    12121 aatgtaaaaa gactacctaa aataggccac tcataaaaat gccatacaat caacggcatc
    12181 gtgatcatag taaacaggac gtgagaggcc gtttttttca accaatctcc atcaaaagaa
    12241 atcaggataa acaaaaaact catgaaaaaa ctaagctgtc cacccgcatg aaaaagaaat
    12301 cgaggatcaa atagtaaaag aagccaaaag acaactgcaa ataagtccat ggtggaataa
    12361 tgcttattca gcaatttgac tagcaatcgc aataaaaaca gcaaactagc acgcaaaaca
    12421 gaaatagaac ttcctgcaat agaaacataa cagatagtta gtagactcaa cggaataata
    12481 ctgacgtgta gtggccatcc gatacgtcga aacaaataat acagccaccc taaataaaat
    12541 tggatgtgta gacccgatag actgaacaga tgcagcaaac cactctccct aaaaatttct
    12601 tctggttctc tgaatcctgc atctttatag ccccataata aagaattaat atatacactt
    12661 gttttttgcg gaaattcaga ttggacaaag gagataaaag atcctctttt tctctgaagc
    12721 cagtttcgcc agtgattttg catggtgatc tgttcaatct tttgaatttg gaaccgtcca
    12781 gaaattctat ggttcctgaa gtaagcttct tggtcaaaat tataaagatt ccgctttttt
    12841 gctggagaga tgaactcgcc ttcagcagtc aattctattc cctttcctcg gaagttaagc
    12901 catgttttca attctgcttc tgtgtttact tgataagaaa gctggagctt ttgtttattc
    12961 tctgataacc ttccttccat tgtcaaaaaa ttaccatttg cacgaatcgt atctgtaaat
    13021 acagtaattt gcgcagatgt tgttttttcc tctaaaaatg aatactcttg tagcctataa
    13081 gaaatggata ccgttatgct acacaaaata cacaagtacg cgataaaacg tccttctttt
    13141 agcgaaaacc ataaaaaaat tccggcaaga agcaaacgat aaccccaatt gtcagtgtaa
    13201 tacaacaaac agataatcat cgacgtaatt gccggaaaaa tcaaccgatt tcgaacatgc
    13261 ctttctaaaa cgctagacat gttttcctgt taattcattt tgaagttttg aaaaatgctg
    13321 tgagtctaaa gcaacttggt gtacagatac acctaattgt tcgatcaacg cttgggcata
    13381 aggatcattt cgatagtcat ggagataatt gatccctttc acaccagctt gcaacaaagc
    13441 ctttgtacag gccaaacatg gaaaatgtgt gacatagatt tctgaacctt ccgtcgggat
    13501 accaagtttt gcacactgta ggagtgcatt catctctgca tggatcgttc gcaagcagtg
    13561 tccatccacg acgtaacacc cttcatctat gcagtggaca tctccactga ctgctccatt
    13621 atagccgcca gcaatgatcc ttttttctcg aacgagtgtc gctcctactt ctaaacgtgt
    13681 acacgtacta cgtaaagata acagtactgc ctgagccata aagtactggt cccaaggaat
    13741 gcgttcatta tgtgacttat cagtaacttt ttctttattc gtatcgcatg cttttgaaga
    13801 tttttctaac atgttttata ttcctccgtt ttttcaagtt gattaaaaag tactcattgc
    13861 ttaaacaaaa tattctttaa atataacgaa tcatcctgtc tgacaaaaga tcctaccctt
    13921 cactgccgaa atgaagtccg tcatccatta gcccattata aaaattcttc gaatccattt
    13981 gttcaagaaa ttccgtataa aaatcgatac ttcacaatca tggcgttttg ctacttctgt
    14041 gagaacagag atatattctt tgatcaactt gttattccga tatttttgtt ttgtctcatc
    14101 gactgctggt ggcgtgataa agaaagcatg atcagaacta tagtcttttt tcagctgaac
    14161 tatcatccac tgcagatttt ttggtacgtt tctttagaaa gttgtttatg ctctgctaga
    14221 tcattcgtcc caatgagaat aaatacttta tctgctgtat agctaagtac atctttggca
    14281 aagcgttctc gtagagcttg actgttattg ccgctgacag ccgtattcat aatatgaatc
    14341 ccagatattt tattttggag atagacattc agcattggtt tttttaatgt ttctttgcga
    14401 acaatcaagc tatttcctat ccaaaaatac tacctcctcg tcctgccaag ctcttagaat
    14461 tggattattc ttagtatcca ttagcgtcca tttattgctc gtgccagtcg attttgaatc
    14521 attctattta attgtaacga agttttttag tttttcaact gtcttttcac cgataccaga
    14581 aatctcctgc aggtcttcca ctttctggaa ggatccgttt tcttctctaa atcgaatgat
    14641 ttcttgcgct ttgactgatc cgatcccagg aatctgctga agttctgatg gagtggctgt
    14701 attgatattt attttgggag gctcggaatc tgctgtaggc tgttttcctt ccgaggatgc
    14761 caactgatca agatgttctg gcagctcttc acctacagca gggatatata ggacttgctg
    14821 atcttctatc ttcgcagaaa agttgattcg atcgctatcc gcttcttcta ataatccacc
    14881 tgcacgcttt agtacatcat agacccgttc ttctgaagaa aaggaataga taccaggctc
    14941 cttcacactt cctttgatat cggtgtacat gacctttgga cgagcatcct cttctacatc
    15001 gtttccttca gctgatggat cttctccact ggaactaaac gaagaaagta tttcttttgc
    15061 agcagtctct tctgtttctc tataaaatag caaatttgat aataaaaaac cacttgcgat
    15121 acaaaagata agaactatca tagacatcca aatgatcaat ttgggtgaag ctagtatttt
    15181 cttgctgatt ttttctatca ttgttctttt ttcaaagaat gaccagtaaa aatcattcat
    15241 tactcctttc atttaaaatt gaagaaacag tgagctctgg caaaatcgtc ccttcaacat
    15301 ctaattacgt caattttttc ttttgttttt atttttatcg tgaaga
//
LOCUS       DASJTC010000065        15280 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.065, whole genome shotgun sequence.
ACCESSION   DASJTC010000065 DASJTC010000000
VERSION     DASJTC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.065"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..737
                     /locus_tag="U4B19_001915"
     CDS             <1..737
                     /locus_tag="U4B19_001915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2077645.1"
                     /translation="ETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLLS
                     YVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVRK
                     NREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDYK
                     NILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENGR
                     DIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            1733..1984
                     /locus_tag="U4B19_001916"
     CDS             1733..1984
                     /locus_tag="U4B19_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077646.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            2067..2339
                     /locus_tag="U4B19_001917"
     CDS             2067..2339
                     /locus_tag="U4B19_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077647.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            2429..2668
                     /locus_tag="U4B19_001918"
     CDS             2429..2668
                     /locus_tag="U4B19_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077648.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            2815..7098
                     /locus_tag="U4B19_001919"
     CDS             2815..7098
                     /locus_tag="U4B19_001919"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HEN2077649.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            7197..7562
                     /locus_tag="U4B19_001920"
     CDS             7197..7562
                     /locus_tag="U4B19_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077650.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            7564..8016
                     /locus_tag="U4B19_001921"
     CDS             7564..8016
                     /locus_tag="U4B19_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077651.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            8630..9940
                     /locus_tag="U4B19_001922"
     CDS             8630..9940
                     /locus_tag="U4B19_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEN2077652.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            9963..10274
                     /locus_tag="U4B19_001923"
     CDS             9963..10274
                     /locus_tag="U4B19_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077653.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            10634..11890
                     /locus_tag="U4B19_001924"
     CDS             10634..11890
                     /locus_tag="U4B19_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEN2077654.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            11887..12432
                     /locus_tag="U4B19_001925"
     CDS             11887..12432
                     /locus_tag="U4B19_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077655.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            12433..12933
                     /locus_tag="U4B19_001926"
     CDS             12433..12933
                     /locus_tag="U4B19_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077656.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            13014..13570
                     /locus_tag="U4B19_001927"
                     /pseudo
     CDS             13014..13570
                     /locus_tag="U4B19_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13590..13934
                     /locus_tag="U4B19_001928"
     CDS             13590..13934
                     /locus_tag="U4B19_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077657.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            13953..14864
                     /locus_tag="U4B19_001929"
     CDS             13953..14864
                     /locus_tag="U4B19_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2077658.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            14943..>15280
                     /locus_tag="U4B19_001930"
     CDS             14943..>15280
                     /locus_tag="U4B19_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2077659.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETL"
BASE COUNT         5359 a         2224 c         3284 g         4413 t
ORIGIN      
        1 tagaaactct aataaaagat gaggaagata agattgagat tctcatcggt aaactaattg
       61 catacagaga tatgaataag ttacttaaca aagagttagc tgataatata ggaattagtg
      121 aagtattact cagttatgtg ttaaatagaa agagaaatcc gtcaaaagaa atgcaaaaaa
      181 aaatcgatat ttttctatta agtaatgaaa aagaaatttt gaaggagatt aatcgtgata
      241 gtgagatatt tagtttatca aaagacgata aaattgttat gggaaaaaga atacgagaag
      301 tccgaaagaa ccgggaagag acgcttgaaa aatttggtaa aaactttacc atctatacag
      361 gcaaaaatgt tataagtaga tgggaaaaag gtattaatat tccagatatt gaaaaactaa
      421 tgaatattgc ctatcttggt aaggtcactg ttccttacct tatgtacgga gaagattata
      481 agaatatact tccaaaagat gaaagagtta gcgattttaa aaagataaat tcttttagta
      541 tgggattaag gatgaggaaa attaggaaag attattatct agaaagggag gagtttggaa
      601 agcttttttc tccttcaatt tcaaaatggt ccatagatag gtatgagaat ggtagagata
      661 tacccaatac taatcgaatt atccaatatg cttatatagg aaatctttct ttagaatttt
      721 tgatttacgg gatataaaaa tagtttacgt cgatacttat taagttatcg acgtttttta
      781 ttatagaaaa aatttataaa aggaggtttc ttgatatgat aagaaacaaa aagggtttgt
      841 tgaaagttgc tttactggca gcttttttct aagtaacttt ttgacaagct caatggcaac
      901 acaagcggaa gaagtaatac aagaactaac tgttatgaat aacagctaga taaaatgggc
      961 tatgtatgtt atccaacaat ttagtaatca ctagaagcct agcgaaagtt cgcttgataa
     1021 gttatagatg agtattgatt tgtagtgttt ccaaatcaaa agggtacaga gccacatgaa
     1081 atatgttgtc ctctgcagcg tcaggatggg aatccatacc atggagattc ggagatctgt
     1141 gagggaatac ttacgtggtt aatatgtcag ttgtgttgga cgtagtaatg gtcatagtgg
     1201 gtagattgac tgaattctta tatgagaaaa tgaaaccaac aagaagatag ggttatttat
     1261 acagaattgg ggatcttttc tggaaaagta atcggataat gcgagattcg tacggtcttt
     1321 tttaaatttc tctaaaaaaa acggcatcga atgggttggg tgattgataa gcaatgaatc
     1381 atagactgat atagaataaa aaatgattct atttatcttt ttacatctat cgttttttca
     1441 agtagccgca aattatatca gcattcaaca atttttgtat acaagttcta gtaaaaggat
     1501 ctgaagaaaa agttggttgt tgtgaagata aaaaaagaat caggttttac tgagtttttc
     1561 ttcaaaattt gtgttattag gttaggacaa agataagaag aagtatgaag atttaaaaaa
     1621 ggaatgagga aaagatagta atttccgatt attggagttt ttttatttat aaagaagaag
     1681 cgaataagtt gtgattccta gatttcgcgc gtgattaatt aatgagcaaa aaatgcggaa
     1741 agttttagtg tttaatacac gaggacaaag tttcttaggt attgaaggca cacgtgtaac
     1801 ggttgatggg tggtgtgatt gtttcgaggt tggaaaacat gttgaactta tggcatttac
     1861 tgagctaaaa gataagaaca acgtggaatt tttcgagagt gatattggtt gggacgatca
     1921 tgaagaagtt tatggaaaat ttattttgcg gaagggaaat tcttatacga atggggaagc
     1981 ataagtagcg atttgttaga gatcattgat aggattgaaa ttgtcgggaa tatttatgag
     2041 aatctagagt tatttgaggt gatagaatga gtgatggttt tgaacaagta tcaataatta
     2101 aaaatcaagt tcgggagatt cttagaaaaa aaggctattt ggtagatagt tattttgaag
     2161 gagattacga aacatgggta ggtgtctatg ctcgtccaga gaataaacct acctatttag
     2221 atcctacgac gtcagaagat gcctatttac aaaataggta tcgagtagat ggttttaagc
     2281 aagactttgc ggagtggttt gaatgggaaa ttgaaaacgg agaagtaaaa gaagaataag
     2341 agtacttgtt gtatcaaaat aagccagtag aacaaccaaa ccagtagcta gtaatgacaa
     2401 taatactgag gaatcggatc attatggtat gattgatatt tctcttccag aagatcttaa
     2461 gccagatcct gtaacaattg gcaatagtgt catgattttt gattcattag atgaagctat
     2521 gtcatatggt tggaaagaaa ttaaggatga aggtagtaat tggtatggca tgagatttgg
     2581 tgatggtgaa gttgccaaaa aagctggtat tccaattggg aaatggacag ttgatttcca
     2641 cgaagcaggt tctttttttg ttctgtaatt atagtgggag ggtaaaatca ttgatgaaat
     2701 gtgttcagat aagagcgcca gataaaaata gaagagacag ttcacgtcgt tatcactttt
     2761 ggtaacggcg ttttgtttta ttatataaaa ttctcaggaa aaggaggaat tcttgtgtat
     2821 gcaaaaacga aaatgaattg gttgaaagca ctcgcattgg tggctttttc tcaattctgt
     2881 ttcttggtgg gaactgctca agcacatgca gaagaaacga cggctataca acctacgatg
     2941 gaagccattg cccaagtcgc ttctgaagtg acagaagtcc aaccagcaga agccggggtc
     3001 ccagctggtg aagccactgt tgaacaggcc cctgtgacag aagcgccaac ggtcaattcc
     3061 aaaacgactc tcgatcagac gattggggaa acacaacacc cagggaatag tcctggtact
     3121 tcacaaccag ttgaagaaac tgcctcaatt acaacccctg cttcagaaac gtccactcta
     3181 gaaggagaaa aagctgccga agcaacgcct tctgtgcagc cagagtcttc tgagaaacaa
     3241 gcggaagaaa agaaacgaat agaagaaaca gtggaacaac cagcggctac tgctgacaaa
     3301 gaaggaaaaa atgaggaaaa cgttgaaaaa acaatctctt caaccgcagc tagtcaaaat
     3361 caaccaacga aaccaaacaa agaatctacg gctagtattg aaaatcaaaa gaaaaaagag
     3421 cagaacattc caaaaacagg tacacctaca ctgttagtaa cagtaccaac caatcgttcg
     3481 gttcgtgttc gccgtgcaac caatgcagct gttttaggtg gtcggaaaat tgattggggc
     3541 gaaaatattg gtgttacttc tagtccgaac aatctaaact tcactttaaa ttatatggtt
     3601 cgttttgaga ttaatggaca agtggcgttt tgtattgagc ctggtgtctc agccgcagaa
     3661 ggtcaagttt atacagctac tgctttgaaa aactatttga aaaattcaaa tgtacggaaa
     3721 aaaacaagct tgatctctta tttaggatat gtcacaaata aagacaaatc cgatgaacaa
     3781 tacattgcgg cacaatttat gatttgggaa gctttgggca caaaaatccc aatcaaaaat
     3841 atccgtgttg attatgatcg tcgaaaagca gaaattcaac gtttgattaa taactttggt
     3901 gaaaaagcaa cttttaataa tcaaacacat acggtaaaaa ttggcgaaac gttgaaagta
     3961 aagaataaaa atgatttttt gaaatatgtg aaagacattc aaaaaccaaa aggtgtcaat
     4021 gtatctattc acggcaatga agtatggatt acagtcacaa aagaggcacc ggataaagca
     4081 aaaattcaat tgaataaaat taaagttcaa ggtgcgccat tagcctatcg tcgtccagga
     4141 gctcaaacaa ttggtgtgtt acatccaaca gaacctaatg cgtctgttat taatttacaa
     4201 attctaaaac aaggaaacat tcgtgtgttt aaagaagatg cggaaactgg tgtgaaagca
     4261 caaggtgcag cttctttaga aaatgcggtc tatggtttgt atcaagcaga tgggaaaaag
     4321 attaaagaaa ttaccttgaa aaaaattaac ggcaaagtac aggcagaaat taaagatctt
     4381 gatcctggca aatatgtttt aaaagaaatc aaagcgccaa aaggttatgt gctttctgat
     4441 gaagcaattg aaattgttgt tgaacctgga aaaactgtca atgcgaccgc aaaagatcaa
     4501 gtgatcaaag gaaccattga tttagtgaaa gttgctaata aagatttagt cgattcaacg
     4561 aatccagata acaaaccaaa attagctggc attgaaatca ctttgacgtc aaaaacaact
     4621 ggcaaagtgg tgaaaaccgt tgtgacagac aatgatggtt atgctacttt tggtaaaaat
     4681 accgttgtct ttgatacgta cattctttct gaaaccaaag gaaaagaagg gtataaacta
     4741 tttgaaccac ttgaagtcac aatttctgaa aatgggcaaa ctttccatta tgttttagaa
     4801 gataaagtaa ttgaacaacg tcttaaagtg gtgaaagtgg atcaagaaac aggaaaagtg
     4861 attccacttg ctaatacaga attcaagatt tttgatactt tagccaatcg ttatgtcaca
     4921 atggagattc caaatgatac agaaacaacg gatatcttta aaaccaatga aaaaggcttt
     4981 ttcctaacta acggtacact tacttatgga aaagatcgct atcgtttgga agaaatcaaa
     5041 gcccccgtaa attacgtttt gaatcaaaac ccattagtgt tctcgattac caatgataca
     5101 gaaccgatca aagtgatcca ttttgcgaac cgtcaagcac ggaaaagtgt aaaattattc
     5161 aagtatgaag aatggaacaa gaagaaaacg ccgctcgcag gtgtgaactt taccttgtat
     5221 aacggtaaaa atcaagcaat tggtgaatat accactgatc aaaacgggga aattttagtg
     5281 aaaggtctag ttgccgatga ctattacttc ttggaaaatg ccccattaga aagtttccaa
     5341 ccaaatacag ataaacaagc attcacggtt gcattcaaac aaaatgcgcc agatgatggg
     5401 gaaacgttag tggtttctgt acgtaattac ttgattccac caaaactaga aacacaagcg
     5461 tcaaataaag ccgatggtga aaaagtggtt gatccattag aaacgatcgt cattaaagac
     5521 agtgtttctt ataccaactt gtttgtcgga aaagaataca ccatttcagg agtattgatg
     5581 aacaaggcaa cgaatgcgcc aattttacaa gatggcaaag aaatcacaag taccttggcc
     5641 tttattgcag acaaagtcaa tggcacaaaa gaccttgaat ttgtggttaa tgctagtgtt
     5701 ctgaaaggtc aaacgatcgt tgtttttgaa aaacttttcc gtgatggcat tgaagttgca
     5761 gcacatgcgg atatcaacga tgaagcccaa agtgtgaaag tgaataatcc aaccgttcat
     5821 accacggctg tctttgaaga cggattgaca gtcgcagacc caacaggatt gtttacctta
     5881 aacgataccg ttgaaattac cgagattctt ccaggaaaag catacttggt caaagggatc
     5941 ttaatggata aagcaaccgg tgaacccttg cttgtgcatg gaaatacggt agaaacgagt
     6001 gttggcttta ttgccaaagc agaaaatgaa acggtgatcg taccatttac ctttgatttg
     6061 acaggtataa atggtcgtga aatcgtcgtg tttgaaagtc tttttagaaa tgatgaagaa
     6121 ctagcaaacc ataaagacat caacgacaaa gggcaaacgg tacgagtaac taacccaagt
     6181 gtacgtacaa cggctacaca taaacccact ggcttgaaag aggttaatcc attaacgaaa
     6241 gtcaccatta cagacgctgt gaaataccac gatttggtcg ttggaaaaac atacacagtc
     6301 aacggtgttc taatggacaa agtaacggaa aaaccagtgc tagtagatgg aaaagaagtc
     6361 acaagtacct taacgtttgt ggcagaaaca acggatggta cagtcaatct agactttatt
     6421 ctagacgcac gtgcgttgcg aggcaaagaa gtcgtggtct ttgaagatct gtttagagaa
     6481 ggcgtattat tggcttctca cgcagatatt accgacaatg accaaaccat caaaatcagt
     6541 aatccaacca ttacgacgaa agcgacgaat aaaaatggcg gaaaagagat cttggccttg
     6601 ccaaatcaaa ctgtgcttga atgggtcaaa gtagacggtc ttgcgattgg tcaaatctat
     6661 cgcttaattg ttcaaggcta cttaacacca gacggcacac catttgaagg cactcatgcg
     6721 gaaaaagtct ttgcggccga taaagaaatg atggaattct tatttgaatt cttagtggac
     6781 ggtcgcaatc ttgcaggaaa aggcttgagt tttgcggaat ggctccaagc gaaaacggaa
     6841 gatgagaaag aaacgtttga agaagtggcg aaacacaatg tagatctaac aaacaaagat
     6901 caaacggttc aaattgtgga agcgccaaaa ccaccgcaac cagtgaaacc agaacaacgg
     6961 gttaaaccag ctactgttgt accaatgttg ccaaaaacag gtgaacaaac aagtagtata
     7021 cttatggttc ttggattagt agtattagtc attgctttag gtataacact ttataaagta
     7081 cgtaaagcca aagattagtc atgaggaaca agcggttagg atttttaacc gcttgtttga
     7141 ttgtaatgta gaaatattta gaatttattt tggtttttaa gaggaggaaa aactttatgg
     7201 cagaaaaagc attgcgtatg aagaaagagt ttttaggtca agtattttta ttgggttcac
     7261 gggaacctaa tccaccaaag gaaattaatg gtgtgtctgg gcgatatgtt caattacaag
     7321 cagagtgttc gcgagaagaa cagcctgctt tttttgttta tctcacagct gaagaggata
     7381 aaagattacg attaccagaa aaagaggtgc gtatttcatt tgaagaagct tattttgttt
     7441 ttcgtaaaga acgagtgaat agtcgtgaaa caaatgatat gggacaaaca gagcgtcgaa
     7501 caactggcta tcaagatgtt ggtgtctttt taactggtgt aaaaattggg gaggttttgt
     7561 aagatgggat taaaatttcc aaataattat gacattacag aagcattaga tgaaacttgt
     7621 ttaggagccg tacgctttgg cgcattagaa gagtctgatc gtgtatttga taatgaaaca
     7681 ggcgattata cgggagaaat tcgatcacag cgagcacaat tagtttttgg aaatccagat
     7741 gaaccaaaga ttgcacgacg agttaaattt ccagcagatg tagatttgac tggttttaaa
     7801 cgacgtgatc ctgtgaaatt aattgatcca gaatatcatg tggtatatat tccagcacaa
     7861 gatggcaatc gtgaatattc agatttagtg atttatgcga aaggattaga gaaagtagaa
     7921 gctccaacaa ataatcctaa tatgaaacaa tcacaagaaa aaggtttaga tcaaaaacaa
     7981 ccaagtgttg attctaagga aaaagagaaa aaataaggtt tttttgaaag aataggaagg
     8041 agtcttccta ttctttttat ctaagataga aggaagggga gattttgaat tcagataaaa
     8101 ccaatcaaat actttgagag attgtgcttg ttatcaaaat atccaacaat tgattgagta
     8161 ttttggagac aatccaacga tttttgtaca aacaaatgca tatatttgtg cggttattgg
     8221 gcagtgtatg tttttacgtg atcttgttcg tttttcatta ccgtaccagt ggaaacaaga
     8281 tcatcaggaa gtgatttgga ttcgtatagt acgaatatgt aatatgtgtg cacaccctta
     8341 taaaaatcga ttaaatcttt ttcgattatg tttttttact aaatgtgata ttcctgtatt
     8401 agttgctttt tgtcaaaagt ttttagcaca ggaaaagtaa aaacagaaag gaggaatgtg
     8461 acaattgaaa gctcattttt tcaagtatcg tggtcggcga attcgctatt tcaatcgtta
     8521 tttatggttt tgctattatg gtttcttttt gtttccattt ttgattctgt taggttggtt
     8581 tagttggaaa gtattttatc cacgtttaag tacgttttcg aattggcaaa tgtatcttat
     8641 tcctggaata agtattgtgt tgtatttgtt gctggttagt gggttaattt tagggttgat
     8701 gcattggtcg cgactaaaag aaggatattt tgcctctgtt tattggcgtc agttacttgt
     8761 tcgaatgtta attgacaatc ggtttgttta tacaaagaaa caagttagtg agaaacaaac
     8821 aagggaaaaa atgagattac caaaagttta tttttatcaa aagaaacaag aattagtggt
     8881 ttcttttcct ttagatggtg ggcgttttca cgaacgattt ttaaaaatgg gtcatttgtt
     8941 atctgaagta tttttgcgtg atttaattcg tgaagaacga gaaaaagccc gaatcaatta
     9001 tgtattcctt tcagattctg gtcggattcc tattgatcaa tgtatagcag aaaatggacg
     9061 tattcattta atgaaaactt tggattgggt atatgatcaa tcaccaaata tgttgattgc
     9121 tggtgcaatc ggtggtggga aaacctattt attgtattct ttaattcaag cttgtttaag
     9181 tgttgggaca gtagatattt gcgatggaaa agcggcagat ttagcagcat taggtgacgt
     9241 cgatgtattc aaaggacatg tgttttacaa aataaatgaa gaaatgatta tttgtttacg
     9301 aaatgcatta aaagaaatga atcaacggta tgcctatatg aaaaccatga aagatcctaa
     9361 atatgaacca gggaaaaatt atgcttatta tgggttacca ccgcatttta tcttttttga
     9421 tgaatgggca gctttttatg gttcgttgga tatggaagcg agaaatagag tcgataaatt
     9481 gattcagcca ttagtattaa agggtcgtca agcagggatg tacattattt tagcaatgca
     9541 acgaccagat gcagaatatt ttccaagtgg tgttcgggat aatttaacat tccgtatttc
     9601 tgttggtcga ttgtctccca ttggttattt aatgactttt ggagaagatt ttaaaaataa
     9661 gccattttac aacaaagatc aacctggtcg tggatatgct tttccaggat ttgatgtacc
     9721 tgtagagttt tttgcgccat ttgtttacaa agattatcac tttatcaaag aatttgagaa
     9781 atttactgaa atgattgtct atgattttac agtaaaagag gatagtcaag atcaaacgat
     9841 gggagatgat aatcagttgt tacaagaaca actagagcgc caaatgtatt tagcaaaaga
     9901 tccaaaagga acaagttttt ctgatgcgca tattttgtaa tcaactaagg aggtgaagtt
     9961 aaatggcaga gagttgttat tatagagtag ctacagcaat acgaatgatt aatccttctc
    10021 tttcttccag aacgttttat gattggttga atcgtattga acaagtaact gattaccgct
    10081 ttttgcgcaa agaacgtgtt tttacaggaa aagttattaa tcaagtattg ctaacgaaaa
    10141 aagatataga acgattgact cgattgtatc attatcgagt agatttagaa gaggatttaa
    10201 ctttgtcaat ttatcgcgtt ttttctcctg aaaaatatag tgaaataaca aaattagatc
    10261 atttaatttt gtagttatta tctatcggga gggctctaga cgtagtctgg tagccgcact
    10321 ctgcctggcg gtgcgcatta aggctgcgaa gcatgggcct ataatgcgca ctcgctagca
    10381 gcgtgtgcag ctcacaaaga ggtcgtgcgc ttgcgcacgg cctctttgtg taaagccccc
    10441 cgatatataa tatggggggc agaaataaca taacacatag aaaaaagttt tataaaccct
    10501 tttgtatcaa gtgattatct aaggtcagca gttgtactac ttttcccaaa aaacgagggg
    10561 gactagttga tatatcggtg tttctaaagg gttaaaaaaa tcgattttgt tattttataa
    10621 aaggagcgag aaaatgacac cgattgagct aaaaagctac agaaagaagt taagtgcaat
    10681 tcataatata agtggagata aaaagccgct atcacaagca agatttgcgc aattgatggg
    10741 ctgctcaaaa gattacatta gaaagatgga acaagggaaa agaaaaatcc cagaggattt
    10801 tgaacaacga atcaaggaac gctttcgatt gtatatgaaa agcggaatag atcttcgagc
    10861 aatgattgat tatttgcgtc tttccttttt tgggacagac tacaaaacag tgattcaacg
    10921 tgtattacga atggatctga cagaatttga aacacgtcca acgggcttgt atctttttga
    10981 ctgtctttct gttcgtggga atatctgggt gttctggcat tcaaaacgaa ctgtaacaga
    11041 aaatgtactt gtgcaatttt caggacaagg ttgccgggaa tatgaggaag tcatgcgtga
    11101 acaacaaaca gattggcgaa catggctata ttccatttgg aaaaatcaag gaaagttgga
    11161 agctggttat acacgtgtgc aatgtagccg attcgatatt gcgttagatg aactttgggg
    11221 attggaaacg gaagaacatt tcgatctctt tgaattacta gaaaaacaaa aagcaggttt
    11281 attagaaatg gactttaaga gattccagaa tattggcggt tgttttttag atgatggcta
    11341 ttttcgaagt gaaggattat ctttgtattt tggatcgcga aagagtcctc tcttttttaa
    11401 tttttatgag aaacgttttg aaattgcgaa ccgagaaaaa atttcagaat tagaagcatt
    11461 aacgaaatat ggtatttata atcgatatga gttacggtta gccaatgaga aagcaacaca
    11521 agcagtagaa gcatttattt taggtgattc accgaaacgt ttaggagaga ttggcgttgg
    11581 tttaattaat gcaaaactta ttgtttggga tagactggaa agtgatgcac ggattgttga
    11641 taaacgatgg gcaaaattat ttcgttcgat ggaagaattg gattttacag taaagccaga
    11701 aaaattctca ttagatcgtt cagaacgctg gtttttgaaa caagtagctc cgacattaaa
    11761 gttgttacaa gaagcagatc gtattttagg aagagacttt gtacaagaag ggattgataa
    11821 tgccgaacta aatgacgagc aacaaaacta tctagaatgg atgggaacgt caggagggaa
    11881 aattgcatga atgcatatac gacaacagaa tggctgcgcc tgttggattt aaaggctgca
    11941 atcgaagcat taaatgaaaa gatggttgat ttgtcttatt ttcgttttcg agttccttat
    12001 attgaacaag cggtaaaagc tggacgttat caggaaaaag agaactggca agaaattgca
    12061 cgcttattag aagttcgtaa gggatacgaa caagaactcg aagagttgga atttagccga
    12121 aggaaaggaa ggctagaatt cattcgcttt tatcgatttt cattgccaat acctgcgatt
    12181 ttagcggtta aaaaaggctg cgataaaatg aagatatatg aaaactgtgt ggcagcactt
    12241 agcagcgaaa aacctcttat ggaagaaatt tcattggtga ctgtcacttg ggaaatgagt
    12301 cgccagccaa cagaagaaca gacatatctt tctttagaag agattgagat agaattagag
    12361 gaaattggtc gctatgccac ttgttctacc tattgtggat cggtcattag tattgctggt
    12421 gtgattgtct aaatgaaaaa aattatgatt tatgttggtg cgtattggtc acgtgatcca
    12481 acagtattag aaaatccaga ggcggtacac tattgcttgc gccaattatt ctatctgtat
    12541 aaagaacgat tagaatcgtt gattcgtcag ttaccatata ctgacagacg tttagatgaa
    12601 cttcttctaa ggtatccagc aatgtataaa cgacgaaaaa atagattgtt accagaagag
    12661 tatcccattg aaaaacatga acttgaaggg cgatttgtgg cttatttcta tgatgatgtt
    12721 cgtatgcgct tagtagaaca acgaatggaa atagaaaatg atcgctacta ttttatcaat
    12781 tattgtaaga agcgtaagta tcaagtaact gatgattttt atgattgtat tttacaagat
    12841 ggggaagtga ttctatccaa aattgctcca agttttcgtg aacttgtgcc gattgatttt
    12901 ttgaaaaaat gtcatttgcg gattcttccg taaaaaagag ctataaaagg aaagaaaagt
    12961 agaagccaaa tcggtttctg cttttttatt ttttttagga aggagaagat ggtatgtcac
    13021 tggaattttt aggagagcca attgaaaagg aagagttcgt ttcgcattat atggatttgt
    13081 ttattcaggt gataaaaaac tttgtgcgat tgatgaattt tatccacgcg aacaagacta
    13141 tctagccgct gaacgaataa aaatcagttt attatgggaa aaattcacag atgaaaataa
    13201 ccgaccgcca gattatcgct atttatcaga ttgtttaacg aataatgtgc tttctaaaga
    13261 atacctattt cacaaacgtg agaaaaatca aatgtcggtt gaacattttt gtgattgtta
    13321 tattgcatta ttgcgtagtc aacagttatt aacagtagaa cgatatggtg cagagtttgg
    13381 ttatgtcttg caagctgagc gtcatagagc tacaagatgc tatgaaaaag aagatcaagt
    13441 gattgatggc tatgaagaat tatgtattcg aaacaatcgc tatttgcaac aacgtttata
    13501 tgaacaattg atgatggatt tttcaaatct ctatagaggg tatgaatgtt cgatgattca
    13561 agaaaggtag aagaaaaggg ggaattttta tgtggcgatt attatttttg gtggtatgtg
    13621 gtatggcatt gttctatgtg tatcgtgtct atctctatcc acgaatggaa cgatggacaa
    13681 gttggtatag agatccttta aaacaacgaa aaggattgaa gcggtatcgt tatgaattac
    13741 ttgttgaatt ttcggatggg cgacaagcac agtattattt ttttgcaaac tatccaaaat
    13801 acaacaccag tgaactattg attcaattat tcaaaaataa tccatatgtt gggttagaag
    13861 aaaacggacg ggagttcttt tatcgttcag ataatattag tgcggcgtct atgaacaaaa
    13921 cacgaattgc ataaaaaatt ggaggaataa tcatgaaaaa aacaacatta gcagtcgtaa
    13981 caacactagg aatttcatta ttttcgtttc aattagggag ttcattggtt tatgcagatg
    14041 aaactgtaga aacacctaag tcagaaattg tagaaacatt gccaggggaa ggattaccag
    14101 atgttgagac agagctaaat ggaggaacaa cggaaaaacc agcagaacct gaacaattgc
    14161 agtcagagaa acctgaaaaa gagccagtga caccagaacc agagtttcct gaatcaactt
    14221 taccgactga ttcgatcgaa gaagaaggtg tgcatagttc aacaacaacg gattcaagta
    14281 tagtcacaac tccggaacaa tcaggaacaa ccagttcttc tgaaaagaat actgattcgt
    14341 caagtacgga acagccaacg atgtcgtcta ccagtgaaga acaaaaagaa gaaccgaaac
    14401 aacctgttga aaaatcagaa gcaattccaa aagaaaataa acctattgtt tcagcaaaag
    14461 aagtcacagt atcggtaacc ccaagcggtg aaattacgac gaatacttct caaggaatga
    14521 gtgtaccgat tgtcactagt aatgtagagg aattgaccca tattccaaca ccaaccaccc
    14581 cattaaaagt agcaggtgat caaacaattg ttggtgtaaa agatggtatt ccattaatcc
    14641 aagatagtga agggaatttg gtaaaggatt tgtcgattcc tgtaaagaaa cttccaagtg
    14701 gcaatattga aatcaaaaca gcagatggaa aaacaaaagt cttaccaaag actggtgaag
    14761 aaatacatgt tgcactttct gttctaggag gagtgttgac aagtattgct ggatttgttg
    14821 gatataaaag aaagagaaaa ggaatagaaa atgagggtaa ttgaatggac tatttttcag
    14881 aaaaacaagc ttatcgttgt caatcgtacg tactagtaaa aacatactag aaggagtagc
    14941 ttatgaaaaa acgacaacta cggcggaaag aaaaagttta tacagagaaa gtaccgaagc
    15001 aaaagaaaat ccattcaaaa aaagcagtga aatggatttg gtgtttggtt ttatttcttt
    15061 gtttttcagg gggcttggca ttttttcaat ccatgaaaac gaaggttcaa gtgtcgtttt
    15121 tgaaagatga aatccaacaa ttacaaaaga aagaagtaca aagtcggtca gaattaacgt
    15181 acacacctga aattgaatcc tttatgaatc gttttgttgc tttgtatatg aatacattcg
    15241 ataatcatga acaagaacaa caacgtcaag aaacattaaa
//
LOCUS       DASJTC010000066        15208 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.066, whole genome shotgun sequence.
ACCESSION   DASJTC010000066 DASJTC010000000
VERSION     DASJTC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15208
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.066"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(94..1137)
                     /locus_tag="U4B19_001931"
     CDS             complement(94..1137)
                     /locus_tag="U4B19_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEN2077660.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1514..2479)
                     /locus_tag="U4B19_001932"
     CDS             complement(1514..2479)
                     /locus_tag="U4B19_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEN2077661.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2713..3558
                     /locus_tag="U4B19_001933"
     CDS             2713..3558
                     /locus_tag="U4B19_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2077662.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3749..4936
                     /locus_tag="U4B19_001934"
     CDS             3749..4936
                     /locus_tag="U4B19_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEN2077663.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4942..5298
                     /locus_tag="U4B19_001935"
     CDS             4942..5298
                     /locus_tag="U4B19_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEN2077664.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5300..5638
                     /locus_tag="U4B19_001936"
     CDS             5300..5638
                     /locus_tag="U4B19_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEN2077665.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5714..5970
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6033..7067
                     /locus_tag="U4B19_001937"
     CDS             6033..7067
                     /locus_tag="U4B19_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2077666.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7060..7746
                     /locus_tag="U4B19_001938"
     CDS             7060..7746
                     /locus_tag="U4B19_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2077667.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7770..8618
                     /locus_tag="U4B19_001939"
     CDS             7770..8618
                     /locus_tag="U4B19_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEN2077668.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8815..9588
                     /gene="sufC"
                     /locus_tag="U4B19_001940"
     CDS             8815..9588
                     /gene="sufC"
                     /locus_tag="U4B19_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEN2077669.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9601..10887
                     /gene="sufD"
                     /locus_tag="U4B19_001941"
     CDS             9601..10887
                     /gene="sufD"
                     /locus_tag="U4B19_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEN2077670.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10884..12119
                     /locus_tag="U4B19_001942"
     CDS             10884..12119
                     /locus_tag="U4B19_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEN2077671.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12106..12576
                     /locus_tag="U4B19_001943"
     CDS             12106..12576
                     /locus_tag="U4B19_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEN2077672.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12581..13972
                     /gene="sufB"
                     /locus_tag="U4B19_001944"
     CDS             12581..13972
                     /gene="sufB"
                     /locus_tag="U4B19_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEN2077673.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14070..>15208)
                     /locus_tag="U4B19_001945"
     CDS             complement(14070..>15208)
                     /locus_tag="U4B19_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2077674.1"
                     /translation="TFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAEAQ
                     TALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILPAF
                     GELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTLLP
                     RKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWEDID
                     YFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMILY
                     GYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLFES
                     GASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4973 a         2836 c         3251 g         4148 t
ORIGIN      
        1 tgttgttgtt agaaggatat ctgcaaaatt aaaaacagtt gtgattcgga cattgttcca
       61 tgcgagaatc acaactgttt tttcatgttc attctaaact ttgatcagcc aaataaatag
      121 taaagccatc actgtttgga gaagcccgac agctaatcca taagataaga agcgtgtacc
      181 ttctttcatg aattttgcaa agtctaaacg cagaccaatt gccgctaaag cagtgatctc
      241 aaaccagctg ctgataaaat gtgccgattg gtcaataact acaggtaaag aaaacaagct
      301 gttgaccaaa caaaagatca caaaccctaa tacataccaa ggaagcggta attttcgttt
      361 tttctgcgta tcagttggcg tttcaaacag agctggttgt ttcttgtgca tgaatttttc
      421 aaacagatag acaacaacaa cgagcatcat aatacgtaga atcttaaata gcatggcgaa
      481 ttcgacaaca gaattgtcaa gtaagcttgc cccagcgact acttgaccaa cagattggag
      541 tgtaccaccc aaaagtgcac ttttttcaat gagatttcca ccaaacaact gtaatccaag
      601 aaaagggaga gtcaacatca tcacagtacc cagtagattg actagtgtga tgatctgtcc
      661 tttttcttcc tctttcgcat ggatggaagg ggcgattgaa gcaatagcag aagaaccaca
      721 aacagcgttt cctccagcca tcatcaaaga agaagtatcg ctgaattgca gttttttacc
      781 aatcaagtaa gcaccgatga tagtgactga catttgcagt aacacaaaaa caattccttt
      841 tacaccaatt tgagcaatcg tttgagctgt gacagttgca cctagtaaaa aaacagagaa
      901 ttctaatagt ttgctttcag caaattttgt gccgattgct agaacaggct gtttaaaaaa
      961 agtatttcct aataaaatac ccaataaaat agcaatagtc gctccaccta actgtggcag
     1021 ataaatagca agaagcctgc tgaaggcagc gatggcaaaa gaaagcagta aaccgggtaa
     1081 gatttcgcgg accgctgaat cctttacaga ttttattgga tgtgacaatg atttcatttg
     1141 atcggttcct ttcgtcaata aatactaaaa gcatactcga acatagtagc actaaaccag
     1201 tattttgaca aaagaacaag aggtcgtgac aaaagtcttg tacgacctct tgtttcgaat
     1261 aaacggtgtt ccagaagtag cttctacgga aataagccga aatttcacaa aaatttgaga
     1321 agcaattttc gtaaattcct tcttatttct tgaagctgac cacttctttt acgacctctt
     1381 ggtaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaaccagta caaaaacatg
     1441 aacagctgtt tttgtttggc cgttcttatt acttgatgca gaacagcttt ttcacaacct
     1501 attgaatcga ttattaagcg atttttttag aaacaaattt tccatgtttg ctatgttctt
     1561 taattaagaa acgtacgtaa tgagcaagaa cgtagaagac aactacactt gcaaacagac
     1621 attccgcaac ttttaagaaa tccaatacgc tactatagat acctaaactg ttgaagtaat
     1681 gaacagtagt aaatatagct gttgcagaga taacaagcgg gaatgtaaag gctgcatagc
     1741 ttggataaaa acgaagcttg aacaaacgtg gaagcataga cagcacaacg aaatacaata
     1801 cttgagataa agcgaacaat gctacgacaa gataaaaatt aggttcagca aagtttttaa
     1861 gataacctgt taggcaaaga gaaccaggtg ctgcaacgat cgtaatcagc ggacgtgtcg
     1921 gatgagccat atttcttacg aaaaagacac gtttgatgat gatcggcaat aagaacgcat
     1981 agcagatcaa tgaaatccaa aagaaaattt ttccaataag tgggaagaag ttcccggcag
     2041 ttacagtgat gaccccaaat ccaacgtaaa tgataaacca gcttggataa attgcttcaa
     2101 ttgaaagatc agctttgata acatggtgat aattgaaata aaagacaagg atcacttgga
     2161 agataacagc gcacagccaa atgaatttga caattggtgc aatactttca tatgaaacaa
     2221 aataagtaca aataaccatg agtgacatcg taaaagttgg tgaaacggag gcgatgattg
     2281 gatcatgcaa cgtttgtttc gtgtgttcga ataagataaa caatttaaca aaaatgaata
     2341 tcataagaat catagagata ccgccagtta gattgcctaa agcaacatgg tgatagtctt
     2401 taaacagatt tccaagggaa gctaacccaa ggatcaaccc gcagatcggg atcggaataa
     2461 ttttcaaaaa gttcttcata tataatattc tcctttttat ttaatagatt ttagtaccaa
     2521 gatgaacgaa tacattgtaa aatgaatagg aatgtatgat tacgagcaag cagacagctg
     2581 attgtcttca tctaagttac tagttggatc agcaactgtt ttgttgtaca tttctattat
     2641 agatggtttt ttgtatattt aaatcaaata gtttttatag taattatcat aaaaagttat
     2701 ggaagtggtg ccatgtttaa gttattaacg acttttcgag cagtatatga aacaagaaat
     2761 ttttccagag cagcagaagt actgttttta tctcaaccag cagtctcgaa tcaaatcaaa
     2821 cagttggagc gggaattgaa tatcgatctt tttcaacgta acggaagaaa agagatgctt
     2881 ccaactaagc aagccgactt attatatgaa cggacactgg ttttgttaga agaatgggaa
     2941 gaaactcagc aaaaattgct caatgaacaa aatgaagaag aagtatgtcg gatcgttgct
     3001 tcccacacct ttgctgtcta tcttttgcct cagttgatga aacatctgat caaaaggttt
     3061 cctaaagtgc attttgagat cgaaatcacg aattctcatg aatcattaga acaagtagcg
     3121 aaacatgaat ccgattttgg ttttatcgaa aagccattag agacttccgg cgtacaaaga
     3181 tactctttga tggaagacca gctagtcatc gctggggacc caaaaagcaa attgtggctt
     3241 gtaagagaag caaactctgg tgtttttcat tacacgcagc gttattttga agagatgaat
     3301 atccaagata agaagatgat cgtgaaaagt aacgatgtaa ttgttgcgtt gctgaaggaa
     3361 ggaatcggtc gctcgatttt gtctaagcgc tctgttccag aaaatgtgcc aatgactccg
     3421 ttgaatttcc atcgatattt ttatttcatc caacgaacca atctggtttc tgatcagctg
     3481 ctagagattg ccgaagagat ccttcgatac aatgaaagca cagaaacttc tcacagcgag
     3541 gagaatctca gctggtagat aattgcatgt atcaaaaaaa gttctatgat acactaaaga
     3601 taagcagtag gcttttgtac tgcatttgct caagcaactg cacaaacgga aagttaattt
     3661 ttcccaaatg tctatggaaa gactttccat tatctaactg ggccgctata ataatcagca
     3721 gtatggtttt taagaaaagg gatgaaacat ggaaaaaaaa ttaaaagctt cgaacgataa
     3781 tcgtattgat tatggcgtga ttttaccagt ctttctgtta tgtttgattg gtctgctctc
     3841 attatatgta gcacttagcc atgatccgaa caatccagca gtcttgaaag gcgtagggat
     3901 gcaagttgtc tggtatttag ttggagcggc agcaatcgtt gtcatcatgc atttgaattc
     3961 caaatggatc tggaaactga ctccttacct ttatggtttg gggttagtgg taatgggact
     4021 cttgctcaaa ttctatgatc agaatttagc tgcttcaaca gggtctaaaa actggtttcg
     4081 attcggtgcg tttactttcc agccagcaga gttgatgaag attgcgtaca tcttgatgat
     4141 ggcactgatc gtaactaaac ataatacgca agtaaaagaa cggacgatga aatcggactt
     4201 ttggctgatt gggaagctct tgatcgtgac aataccggtt ttagcattga tcatggccca
     4261 agatgacttt ggaacgatgc ttgtctttct tgcgatcttt ggcgggattt tcctgatgtc
     4321 tggaatctct tggcgcatca tcgcacctgt agtaatcttg gcagtagtcg ttggagcagg
     4381 aacgatcttc ttggttacga ctgaaggcgg aagagacttg ctttataaag ttggattcaa
     4441 atcttatcag tttgcacgta ttgattcatg gctggaccct ttccatgata cgtcaggaat
     4501 gagttatcag ccggcccaag gacttttggc aattggtacc ggtggcttgt ttggtaaagg
     4561 attcaacgta agcaatatct atgtgcctgt tcgtgagtcc gatatgattt ttaccgttat
     4621 cggtgaaaac ttcggcttta tcggtggtgc attcgtcatt ttcttgtatt ttattttaat
     4681 ctatcgtatg attcgggtgt gtttcgatac gaataatgaa ttttacgcct atatcgcaag
     4741 cggtttgatc atgatgctgc tgttccatgt ctttgagaat attggagcaa atatcggatt
     4801 acttccttta acagggatcc cattgccttt cattagtcag ggaggatcct cgattttagg
     4861 gaacatgatc ggtatcgggt tgatattatc gatgagatac caaaatgaag cacctgcaag
     4921 atcttcaagg aggtaagaaa attgtacacc ttttactggt acccgaaatg ttcaacatgc
     4981 aaaaaagcca aagcgtggtt agaccagcat ggcgttagtt atcaaaccgt tgatatgatc
     5041 aaagaagtac cgaaggcctc aacgattgag acttggctag aaacgagtca agtaccaatc
     5101 cgtcgcctgt tcaacacaag cgggatgaaa tatcgggaac ttggactgaa agatcaagta
     5161 ggcgagttta cggcttcaaa agcgagccaa gtcttatcga cagatggtat gctgatcaag
     5221 cgaccactga tcttacgaga ggaccagctt gttgcaattg gattcaatga aaaaatttat
     5281 gaaggagaac tgatataaaa tggcaggcaa gcatttgaag aaaaaggaca acctttggat
     5341 cttatttaat ggaaaagaat actgtgttgg tttgacaaat gaagcgcaag aagaacttgg
     5401 agacatcacg tttgccaatc tgccaaaagc aggacaatcg ctaaaaaaag atgaacctct
     5461 gatcgaagtt gaagcggaaa aagcggtcaa tgaatttgcc agcccgctaa cgggtacagt
     5521 ttcttctgtt aatgataaaa tcggtgaaga tatcaatatc ttgaatgatt cagatgaaat
     5581 gaatgcgtgg atcttaagtt tcaaagatgt tgatccaaaa gaatttgacg aattataaaa
     5641 tcagaaaatc acttgaaaaa agttgacact gattcttcca gttgctataa tcataagcaa
     5701 tcctatatga aacgagaaag ctttgaaaag aagagtacat gtcacggacg tttgaagaga
     5761 gccctgtttg ctgaaaatgg gtaacgaaaa tgagatggaa gatggtctta gagcagagta
     5821 agcgagcgta agcaaactta ctacgggagt gcccgttaca gcgcgacagt atacggaaag
     5881 aagctaagtc caagtactgg aagaggctgt tattgcgaag taacggtaaa tcaaggtggt
     5941 aacacggaag ttcaagcttt cgtccttttg cttaaggcaa gagggcgaag gcttttttgt
     6001 ttgaaaaatt ataagagaaa gatgtgaatg gcatgtcatt aatccaacta acgaatgtca
     6061 aaaagacatt caatgggaaa aacggaaaca tccaagccgt caatgacgtt tcactaaacg
     6121 ttgaaaaagg ggatatttat ggaatcgtcg gctattctgg cgcaggaaaa agtacattag
     6181 tccgtctcct gaatggactt gagcttccca cgagcggcga agtgatcgta aacgatcaag
     6241 atattacaaa attaaaaaac aaagaattac gtacattccg aaagaaaatc ggtatgatct
     6301 tccagcattt caacctgtta tggtcaagaa ctgtacttga aaatatccag ctgccgctgg
     6361 aattagctgg cgtaccaaag agcaaacgta aagaacgggc agaagaactg ttgcgtttag
     6421 tcgggttaga aggacgcgga caagcatatc cttcacaact ttccggcgga cagaaacaac
     6481 gcgtcggtat cgcacgagca ttggcaaatg atcctgagat cctgctttgt gatgaggcta
     6541 ctagcgcatt ggacccgcaa acaacagaag aagtattaga cttattgtta gcgatcaata
     6601 agaaattgaa tttgaccatc gtcttgatca cacatgaaat gaatgtgatc cgcaaaatct
     6661 gcaacaaagt cgctgtgatg gagttgggga aagtcgtaga agaaggagac gtattgactg
     6721 tcttcagaca accgaaaaaa gaagtaacca aacgctttgt acaacaagac atcgaaccag
     6781 aagctgattc gacagaacta ttagctgaac tcatcaaaga aaatccaagc ggccgtttgg
     6841 caactttgac ctttagcgaa agaaatgcca acgagccagt catttcccaa gcgattcgac
     6901 gctataacat cgatgtcaac gttgtgtatg gaaagatcaa acaagtgaaa gaaggttcct
     6961 ttggttcatt gactgtcttg atgactggtg aaaccaaaga attagatcaa gcagaagtgt
     7021 tcatcaaaga acaaggagta ggaattgagg tgatccatcg tggataaaag cttttcagaa
     7081 acatatctag attttaaaca aatcaacccg gaaacgatga atcaggctat ccttgataca
     7141 ctatatatga cattaatctc aatggtctta gtgacgatca tcggattagt tttgggattg
     7201 atcctttact ctattggacg caaaaacaaa ccgtatgctc gaatcattta tggaatcgta
     7261 tcgatcatca gtaatatttt ccggtcaacg ccatttatga tcctgatggt tttgatcatt
     7321 ccatttacta aagcaatcgt aggcactatg ctaggtgcgc aagcagccat tccagctctt
     7381 gttttatccg cagcaccatt ttatgcccgt ctagtagaga ttgcttttcg tgaagtcagc
     7441 cctggtgtac tggaagcagc agatgcaatg ggagcaagtt actgggaaat catctggaag
     7501 gtcttgatac ctgaaagtat tcccgcattg atttctggat taacagttac aacgatctcg
     7561 atgatcggat ttactgcgat ggccggcgcg atcggcgcag gtggacttgg aggtcttgct
     7621 tggcaggaag gctaccagcg tggtaatcta acagtgacat tcgttgcgac tttaattatt
     7681 ttactaatcg tatttatcgt tcaaggaatc ggtgatttct taactaagaa aacagacaaa
     7741 agataatagg atataggggg atgtaggata tgaaaaagaa aatttttgga tttgcagcag
     7801 cgcttttgct gactgtagga ttagcagcat gcggtaacaa cagcgacagc aaagattcag
     7861 caaataaagc agacgacacg acattgaaag tcggcgcttc accgacaccg cacgcagaga
     7921 ttttggaaca tgtgaaacca ttattgaaag atgaaggaat cgatcttgaa atcgtcaaat
     7981 tcgatgacta tgtattaccg aaccaatctt tggaagaagg cgacattgat gccaattact
     8041 tccagcatat tccttatttg aacaaagaaa tcaaagaaaa aggatatgac tttgtcaatg
     8101 ccggtgctgt ccacatcgaa ccaatgggct tgtactctaa aaaagtcaaa gatgtttccg
     8161 aattaaaaga tggtgcaaca atcattactt caaattctga atcagactgg ggacgcatca
     8221 tcacaattct acaagatgct ggcttagtca aagtcaaaga tggagtagat ctggaaacag
     8281 caacattcga tgatattgaa gaaaaaccta agaacttgaa gttcaaccat acgatcgacc
     8341 catcactttt agcttctacg taccaaaatg atgaaggtga cctagttgca atcaacgcaa
     8401 acttcgctta cggcgcaggg ttgaatccat tagaagatgc cgtattgctt gaaaaggaca
     8461 actctcctta cgtgaatatt attgcgactc gtaaaggtga tgaagacagt gacaaaatca
     8521 aaaaactgat tgaagtgctt caccgcaaag aagtccaaga atggatcaca gacaaatggg
     8581 gcggctctgt taaaccagta aaggctgatg caaaataagg tatggaacaa aaaagatgag
     8641 aaataagtct gtaatggctt atttctcatc ttttttatac ctattctaat ttagaaatca
     8701 aaattgttta ataataaata aaagatattt gattttagta aggaaggtca ttgcgcttca
     8761 ttctcaatta gtctaaaatg agtgagaacg aaagagaatt tgggggtata atgcatgtct
     8821 gtcttagaaa taaaaaatct acatgtgtcg atcgaggaaa aagaaatcct taaaggcgta
     8881 aacctaacaa tgaaaaccgg agaaatccat gccatcatgg gacctaacgg cacaggaaaa
     8941 tctaccttat ctgccgctat catgggcaat ccaaactatg aagtaacaga aggcgaaatt
     9001 ttgtttgacg ggaaaaatgt tttagacttg gaagtagatg aacgtgcgcg acttggctta
     9061 tttttagcta tgcaatatcc aagtgagata ccaggtatca cgaatgcgga attcatgcgt
     9121 gcagcgatca atgcgaaacg tgatgaaaac gacaaaattt ctgttatggc atttttgaaa
     9181 aagttagatg aaaaaatggc tttactaaat atgccagaag aaatggctga acgttatctg
     9241 aacgaaggtt tttctggcgg agaaaagaaa cggaatgaga tcctgcaatt gttgatgtta
     9301 gaacctacat ttgcaatttt agacgaaatc gattcaggtc tggatatcga tgctttgaaa
     9361 gtcgttgcta aaggagtaaa tgaaatgcgc ggagacaatt tcggtgcatt gatcatcacc
     9421 cactatcaac gattattgaa ctatatcaca cctgatgtcg ttcacatcat gatggaaggc
     9481 cgagtagtca tgacagggaa tgctgaccta gctaaacgtc tggaagcaga aggctacgct
     9541 ggtatcagta aagaattagg aatcgactat aaagaagaag aagcgtaagg gggagcaaga
     9601 atgaaagaaa aaaactggct cgattatctt gacgcggtaa atgatttttc tttaagtaaa
     9661 ggcgaacctg attggatgag aaccttccgt caagacgcac tagcgaaagc agatgaactg
     9721 ccattgccgc atatcgatcg ggtgaaattc catcgctggt cgttatttga tgtgaaagaa
     9781 acacaaacca tcagtgaaac aggcacgatt ccggcatttg atgcaatgaa agacaatcct
     9841 gtgcttgtcc aacaaggttc atggacgatt tttgaacaac tgcctgtgga attagcagaa
     9901 aaaggagtca tttttaccga cttgttcact gcaatgatcg aatatccaga actagtccaa
     9961 gaatattata tgaaaaaagc agtaaacatg aacgaagacc aattgactgc actccatgta
    10021 gcttttatga acagcggtat tttcctgtat gtgcctaaaa atgtcgtgat cgatgaacca
    10081 ctcgagtcct tattcattca agatggcgca tccgatgagc acttcttcaa gcatgtgttg
    10141 atcgtggcag atgaacatag tgaattttct tatctggaac gtttccagac aaccaaagaa
    10201 caagtagcaa aatcatcagg taatatcatc gtggaagtca tcgctaaagc tggttcaaaa
    10261 atcaaatatt cagccgtaga tcaattagga gaaaatatta cttcttatat gaatcgccgt
    10321 gggcacattt tacgagatgc ttctgtcgac tgggcaattg gtgtcatgaa tgacggtcat
    10381 gtgatcgctg actttgactc agatttagca ggagaaggtg cacacgcaga agtcaaaatc
    10441 gtggcaatca gctcgggcag acaaatccaa ggaatcgata cacgagtaac caataaagcc
    10501 cctcatacga tcgggcatat tttgcaacac ggagtgatcc gagaaaaagg aacattgacc
    10561 tttaatggta tcggccacat cttgaaaggg gcaaaaggtg cagatgccca acaagaaagt
    10621 cgtgtcttga tgctttctga taaagcacgt ggtgatgcga atcctattct tttgatcgat
    10681 gaaaacgaag taactgctgg acatgctgcc agtgtaggac gagttgatcc agaagaaatg
    10741 tactatctga tgagtagagg attgcataaa gaagaagcag agcgcttagt gattcgagga
    10801 ttcctcggtt ctgtcttaac agcaatccct gtagaacaag ttcggaaaga attagtcgaa
    10861 gtaatcgaag ggaagttgaa tggatgatcg atgctaaaaa gctacgcaat gattttccta
    10921 tcttatttca agacgtcaat gatgaacctt tagtttatct ggataatgca gcgacgaccc
    10981 aaaagccaaa acaagtactc caggcattag cgtactatta cgaacacgat aacgccaatg
    11041 ttcatcgcgg tgtccatacg ttagcagaac gcgcgaccca tgattatgaa caagcaagag
    11101 aaaaagtacg ggcgttcatc catgcaaaag aaacagcaga agtgctgttt actcgtggta
    11161 cgacgacaag cttgaattgg gtagccagaa ccttcggcga ccgctatatc aaagcaggag
    11221 acgagatagt gctctcttac atggaacatc attctaatgt tatcccgtgg cagcagttgg
    11281 caaaacgaac aggtgctgtc ttgaaatata tcgaactaac agaagaaggc ttcctggata
    11341 tggaggatgc agcgaaaaag attacggaca aaaccaaaat cgtctccatt gctcatgtat
    11401 ccaatgtctt aggtgtcttc aatcctgttc aagaattagc tgaacttgtc catgaaaacg
    11461 gcggggtcat cgtagtagat ggtgctcaat ctgccccgca catggccatt gatgtccaag
    11521 cactagactg tgatttcttt gctttcagcg gacacaaaat gtgcggacca acaggtattg
    11581 gtgttttata tggtaaacga aaatggttag aagaaatgga acctgttgaa tttggtggcg
    11641 aaatgatcga ttttgtcgaa ttatacgaca gtacatggaa agaactacct tggaaattcg
    11701 aagcagggac accaaatatc gccggcgcta tcgcattagg ccatgcaatc gattatttgc
    11761 aaaaactcgg tatggacaat atccatcgat atgaagaaga actagccgct tatgtgttgc
    11821 caaaattaca agcaattgat ggattgactg tttatggtcc acaagacccg aattttcgga
    11881 caggagtcgt tgcattcaat ttggatgggt tacatcccca tgatgtcgcg acagctttag
    11941 acatggaagg ggtagccgtc agagcaggtc atcattgtgc gcaaccgcta ttgaagtacc
    12001 ttgaagtatc agcgactgct cgcgcgagct tttatttcta caacacaacg caagacgccg
    12061 accgtttagt tgaagcaatc atggcaacaa aggagttttt ccaacatggg actatctaaa
    12121 ttagacaatt tataccgtca agtgatcttg gatcattctt cccatccgca tcatcgtgga
    12181 agcttgactg actcgacaag caagatcgaa ttgaacaacc ccacatgtgg agatgtgatc
    12241 gaattgcaag tcgatgtaaa agacggaatc atcaaaaata tcgcctttga cggcagtggc
    12301 tgctccatca gtacggcaag tgcctcgatg atgacagacg ccgtcatcgg aaaatcaata
    12361 gaagaagcag aacatttaac tgtagacttt ctacagcttg tccaaggaaa agaagtcaaa
    12421 gatcccgacc aattaggcga tgctgctatg cttggtggtg tagctaaatt tcccgctcgg
    12481 atcaaatgtg cgacacttgc ctggaaagca ctaggaaaag caatggaaaa cgatggtacc
    12541 gcagctttcc atcattacca caaggaagag gagtgagaac atgggcgtac cagaattaga
    12601 agaatataaa ttcgggtttc acgatgatgt cgaaccggtt ttcagtacag gagaaggctt
    12661 gacagaagaa gtgatccgtg agatgtcaag aatcaaaggc gaaccagaat ggatgcttga
    12721 attccgtctg aaatctctag aaacatttaa caaaatgcca atgcaaacat ggggccccga
    12781 cctttcagac atcgatttcg atgccatcaa atattaccaa aaaccaagtg acaaacccgc
    12841 tcgtgactgg gaagacgtac cagaaaaaat caaagataca tttgaaaaga ttggtatacc
    12901 agaagctgaa cgagcttatc tagccggtgc ttccgcacaa tatgaatcag aagtcgtgta
    12961 ccataatatg aaagatgaat tcgaaaagtt aggaatcgtc tttactgaca ctgactctgc
    13021 tttgaaagaa taccctgaat tattcaaaga gtacttttca aaacttgtac cgccaaccga
    13081 caacaaatta gcagcgctga attcagccgt atggtcaggt gggaccttta tttacgtgcc
    13141 aaaaggcgtc aaagtcgatg ttccattaca aacgtacttc cggatcaatg cggaaaacac
    13201 ggggcaattc gaacgtacgc tgatcattgt tgacgaagga gcaagcatcc actatgtcga
    13261 aggttgtacg gcacctactt attcaagtaa cagcctgcat gccgccatcg tagaaatctt
    13321 tacaagaaaa gatgcgtatt gccgttacac aacgatccaa aactggtcag acaatgtgta
    13381 caacttagta accaaacgag caaaagcata cgaaggcgca accgttgaat ggatcgacgg
    13441 aaacctaggt gcaaaaacaa cgatgaaata cccaagtgtt tacttagatg gaaaaggtgc
    13501 acgaggcacc atgctctcca tcgcctttgc aggagcaaac caaatccaag atacgggtgc
    13561 aaaaatgatc cacaatgcac caaatacatc tagctctatc gtctctaaat caatttcaaa
    13621 agacggcgga gaagtaaact atcgaggaca agtaaccttt gccaaaaaca gtgcgggttc
    13681 gatctctcat atcgaatgtg acacgatcat catggacgaa tggtcgaaat ccgatacgat
    13741 cccattcaat gagatccaca acagccaagt ctctttagaa cacgaagcca aagtctcaaa
    13801 aatctcagaa gaacaactct actatctgat gagccgaggc ctgaccgaat ctgaagcaac
    13861 tgaaatgatc gtcatgggct ttgttgaacc attcaccaaa gaacttccaa tggaatatgc
    13921 agtcgaactc aatcgattga tcagttatga gatggaaggg tctgtaggtt aataactatt
    13981 tcattgctcg aaacgttgat attaaagcgt ttcgagcgtt tttttatttg tattttaatg
    14041 gttggctacg tttttggcta cgtttggaat tattcgccca taaattttgc aaatcggtca
    14101 ccagttttct taacagattg tggtgtgaca tgagcataga tattcatggt agttttaata
    14161 tctttatgac ctaatcgttc ctgtacttct tttatcgacg caccactttc aaacaataag
    14221 ctacagtggg tatgcctaaa tccatgtgga gtaatttgag gctccatcgg tgtctttttg
    14281 taaatccagt ctaaccaatc gtttactact tgggggtagt acaacttgtt tgtgtttgta
    14341 gtgaaaagaa actgttttgg tgaattcgtg ttatagccat ataagatcat tattttcatt
    14401 tgttcttgtt gccaaatacg cagttgttta agtgtaaaat catcaagctg aatcgttctt
    14461 gatgaatttt tagttttagg tacttgtaga actacttgat taaactcgtc ttgagcgata
    14521 gttttaccaa ttgttattgt tttattgaaa tagtctatat cttcccattg aagcgccaat
    14581 gcttcgcttt tacgcattcc tgtataagct aataaacgga aaaaagtgta cagtttaact
    14641 gaattatttt ctttagtatt ctgtaaaaac agttgtaatt gatctttcgt gtaaaacttc
    14701 aagggttgtt cttcttcaat ttttcttgga agcaatgttt tagacatagg attatcctta
    14761 cagacttcca ttgctactcc aaatttaaat atttgagcag ttgtttttct caagaaacca
    14821 tattgtttta atgttttgtg ccattcattc acctgttttt ggcaatagct aacacttatt
    14881 ttatccaatc gtaattcccc aaaagcaggt aatatatggt ttttgcaaaa acgttgagca
    14941 acggcaacag aagacggttt tactcttgtt ctgtagttat caatccataa ttcatacaac
    15001 tcttgaaaag ttgtaacatt gattttaaac tcttgttttt ctttaaagtc tacaagtagt
    15061 ctattaagtg ctgtttgcgc ctcagctttt gatttgaatc ttctacgctt tgtacgtata
    15121 tcttttccag tcttgggatc tttccctaaa taagtttcaa atccccaata tttttcgcca
    15181 ttttgtaatt tataactaaa aaatgttg
//
LOCUS       DASJTC010000067        15207 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.067, whole genome shotgun sequence.
ACCESSION   DASJTC010000067 DASJTC010000000
VERSION     DASJTC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.067"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            376..708
                     /locus_tag="U4B19_001946"
     CDS             376..708
                     /locus_tag="U4B19_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2077675.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            1004..1159
                     /locus_tag="U4B19_001947"
     CDS             1004..1159
                     /locus_tag="U4B19_001947"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEN2077676.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            1435..2775
                     /locus_tag="U4B19_001948"
                     /pseudo
     CDS             1435..2775
                     /locus_tag="U4B19_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3016..4632)
                     /locus_tag="U4B19_001949"
     CDS             complement(3016..4632)
                     /locus_tag="U4B19_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN2077677.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            4750..4968
                     /locus_tag="U4B19_001950"
     CDS             4750..4968
                     /locus_tag="U4B19_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2077678.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5163..5624
                     /locus_tag="U4B19_001951"
     CDS             5163..5624
                     /locus_tag="U4B19_001951"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2077679.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5817..6056)
                     /locus_tag="U4B19_001952"
     CDS             complement(5817..6056)
                     /locus_tag="U4B19_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2077680.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(6080..7603)
                     /locus_tag="U4B19_001953"
     CDS             complement(6080..7603)
                     /locus_tag="U4B19_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEN2077681.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(7621..7743)
                     /locus_tag="U4B19_001954"
     CDS             complement(7621..7743)
                     /locus_tag="U4B19_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN2077682.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(7773..8756)
                     /locus_tag="U4B19_001955"
     CDS             complement(7773..8756)
                     /locus_tag="U4B19_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEN2077683.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(8780..8929)
                     /gene="rpmG"
                     /locus_tag="U4B19_001956"
     CDS             complement(8780..8929)
                     /gene="rpmG"
                     /locus_tag="U4B19_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEN2077684.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(8950..9327)
                     /locus_tag="U4B19_001957"
     CDS             complement(8950..9327)
                     /locus_tag="U4B19_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077685.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(9359..9538)
                     /gene="rpmF"
                     /locus_tag="U4B19_001958"
     CDS             complement(9359..9538)
                     /gene="rpmF"
                     /locus_tag="U4B19_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN2077686.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(9553..9822)
                     /gene="rpsN"
                     /locus_tag="U4B19_001959"
     CDS             complement(9553..9822)
                     /gene="rpsN"
                     /locus_tag="U4B19_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN2077687.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(10345..12111)
                     /locus_tag="U4B19_001960"
     CDS             complement(10345..12111)
                     /locus_tag="U4B19_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077688.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(12071..13825)
                     /locus_tag="U4B19_001961"
     CDS             complement(12071..13825)
                     /locus_tag="U4B19_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077689.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(13935..14504)
                     /locus_tag="U4B19_001962"
     CDS             complement(13935..14504)
                     /locus_tag="U4B19_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEN2077690.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(14522..>15207)
                     /locus_tag="U4B19_001963"
     CDS             complement(14522..>15207)
                     /locus_tag="U4B19_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2077691.1"
                     /translation="IEVPYIEKVSADVLKVEEITYENILKDINFDVKVGDVIGLVGKN
                     GVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTESVRSELALGNEI
                     VSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNLFLFDEPTSGLDY
                     INMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKKDIKLRVEDSTLI
                     EQIFNDITK"
BASE COUNT         4841 a         2957 c         2289 g         5120 t
ORIGIN      
        1 attttaatta tcaacgtttt ttaattcgtt tttagcttaa aaataacttt tgtcacagtc
       61 tcactcctaa aattacttta tttacgtatg aagtgggcag gaggtcgaat ccagaactgg
      121 gctatggtac ttcaacgagg acttctcctc tagaattgaa aagcatgcga tttaccttcc
      181 gtaacgacgg tagatttatt caactatgtg tccaatcaaa atttgtctga tttcaaaaat
      241 agtcaagaga attttggttg ttaaaaactc ttatctatca acttccacag tttagttgac
      301 aaaccctgat actaagttgg gctatgatac acttttcttg actatcagtt acgttaccgg
      361 aaggaatgaa ctctattgag aaaaacatat gatcgtaaat ttaaactaaa gatttcacag
      421 gatatccttg agaaaaaagt aactaccaag aaaattgctg aagaatacaa tatctctcgt
      481 ccaacaattt cccgatgggt ttcagaatat cgtcgatacg ggaaaaatgc ttttgctgga
      541 caaggaaaaa gattacctga taaagcagat ttctacattc ttgaacagga aaataaacga
      601 ctaaaagagg aagacgagtg ttctacgaac acaaagatcg atacggatct ccagggatca
      661 ctcaaaaact ttacgatgaa ggaatcgaaa tcaataaacg tgttgtagct gttcttatgc
      721 gtgaaatgaa tttatgcaca agaggatttc atcgacgcaa atcttctcat ggtagagaaa
      781 aaacaatcaa agaaatggta aaaaaatgtt gaatcgataa tttgaacaga accaaattga
      841 tgctgtttgg gtgatggata ttacttacat tccatgtaat gacggtcgcc tctatttgtc
      901 cacctatatt gacttagcaa ctcgtattct acgttactat atggtagatt cacacatgaa
      961 aaaagagatt gtcgtccgtc cattacaaat ttattaagga gaattgccta cggtaattca
     1021 ttccgataga ggtagccagc atcggtcttt tatttatcaa gagctgttga acgaccacca
     1081 catcacacat tcagtgagcc aaactgggac tcctgttgac aatgcagtaa ttgaagcgtt
     1141 tcatcgatca atcaaatgag aattaatcga ctccaacaaa tataaaagta aaatagagat
     1201 gaaggtgtta atccaagatt atttagaaga ctactaccca aacaaaagaa tccatacaaa
     1261 atttatgatg actccttgac aatttgaagc gaagaaaata agggatctat aatatctagt
     1321 gttggtgacg ccttctgttg ccaatacttt ttcttttttc taataaacgc aaacagacac
     1381 cttaaaatcc tttagaataa agtcgacgaa aaccattcaa aggagtgttt caagatgtcc
     1441 tattcagagt ttaccaaaga gttactagat attctagacc taaatcttat ctttcatgaa
     1501 gatgcctttc gcaaagagcg aatcaatgac gaaacatgtt ttgtttttga tggcacattg
     1561 acctatcaac cggaggaatg ctttcatcgc cactatcaaa atgaacaaac gatcattaaa
     1621 tagggctgga aaaaagtatc catcttattg aatgatgtca gtaactacaa gaccattctg
     1681 cggattaaca aacagcgctt caaatgtaaa cactgtggaa agactttttt agcagaagat
     1741 tcagttagtg atcgtcgctg ctccattgct cgcagagtca aacaagccat tcttgagcta
     1801 ttaagcgaac ctctttctat gtctttgatc gcacgtatga agcacatttc tccgaccact
     1861 gtcattcgaa tacttcgcag ccttcggcct aaaaccattt ctttgagcca gctgttacca
     1921 gaagtggtgt gtttcgatga attcaaatca gtgaaaaatg tctcgggagc catgagcttt
     1981 gtcatgatgg acggcaagac ccatcaactg attgatattg tggaaaatcg gcaactcaat
     2041 cctttacgcg actatttctt gcgctaccct ataaaagtcc gtgaacaagt gcgtctagtg
     2101 gtttcagatt tctatacacc ctatcgcaca ttggtgaaag agtgttttcc gaatgcccga
     2161 atcgtcgccg atcgctttca tattagccaa cacattggtc gggcttttac caaccaccga
     2221 attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt cgaagcactt aaagaaatac
     2281 tggaaactac ttcaaaagaa tgcgtgggaa ttgaaaggtc agcatcgcta ctggcgtccg
     2341 tcatttcgag atcatctgac agaagcagag atagtcgatc ggctattatc ttatgatgat
     2401 tccttaaagc gaggctacga agtctaccag gattttcttt ccgttatcag aagacaagat
     2461 gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg agttgccaga gcactaccaa
     2521 ccagtgttca cgaccttcaa gaaataccaa acagaaatca aacgagcgtt gcgcgttccc
     2581 tattccaacg gccctataga atgtttgaat aaccatatca aagtactaaa acgaattgcc
     2641 tatggctata gaaactttca aaactaccga gaacgaatct ttttatatcg aggaaaatat
     2701 tttaagaaaa ctaagaatac tacccaactg actaaagcca gaacaacaac acggctagac
     2761 aagatagctg tctagccgtg tgagtatctc gattattcta ttgaatttct tcaccaacac
     2821 tcattgacga agagccaaaa taagtaatct aaccgactgt taactaaacc attgcaggtc
     2881 ccgttaaaga tacttgtgcc tgttgtcacc gcttcttttt cttgtccaca agcgcttaac
     2941 acacttaata aagcccctgc tattattaat aataatatct ttttcttatt aaaaacctcc
     3001 tcatctattt ccagattagt aaatgtagta atctgtgctt tcttttttct caatactaat
     3061 aactaactta tggggtaaac aaatactcgt ttgaccgggt tttgagatcc atccagttct
     3121 aaccgcgatt tgatcaggac tattatcttc tttaactcga attcttccat ctttaatctc
     3181 aacaatatta tattgtccct tcgcaggatg ataattcacg agtttatgat tgatcttatc
     3241 caaatcgaag cgatccattt ccttgccatc aattcgaacg atggcatatt tgatagcatc
     3301 ttcagttgtt tcattctcct tttctataga gaatacaaat aagggaataa aagaggtaat
     3361 aatcaaaatg ccaataatta tgacatcgcc tatcttgaca ccagcttttc ttaaaaattt
     3421 cattaacgat atctggctaa acatattaga ctttttccat ggaaatggtg aaatttttcg
     3481 gaaattatct gcattaagag aatcagttaa acaaccctca agttgttcct taatacattg
     3541 ttgatccatc ttcataccaa taaaaactag tttattaatg cgttcaccaa acaacggatg
     3601 ccaggcttcc ttcaattcgg gatttttttg tataatcatt tgacactcaa ttttaggtaa
     3661 actagctacc caataattta atgattcaat acttttatag ctacccgatg tttcaaagat
     3721 cgtcgaaaaa tcactatgtt gggcaaacca aagaaaccct tttgctcttg taatattgcc
     3781 aggaaaattt ttatccgtaa gatgggcaaa tttttgtgcg tcaaggagta accgatttcg
     3841 atagataaaa cttgagatac catattcttc tgtttcagga atatgatttt gatagccaag
     3901 ctctaactct ttgaaccagc ccactgaata ggaagtctca tctatattaa aaagatttgt
     3961 atttagaatt ttcgataagt ctacttcacc tttaattgtc ctaattattt tggcattgga
     4021 ctgtaactta gcaattagac cgttaatttt gtaaatcgtc ttggttgaca ctaagtcact
     4081 attatttaaa agtaatatgt tacaacactc gaattgatca attaacaagc cacgaatatc
     4141 ctgttcttct tcaactacat taccttcagc ttttctttcc tgaatctttt cacccgtttc
     4201 gaaattatcc caaaaacgtt ttacatccac tactataata atggcaccaa tctctaatcg
     4261 ttgggtcaaa tcaatgtcta aagggttttt agctaatata attgcttgaa taattggaat
     4321 tggtttgctg attcctgaag cttcaataac aatttgattc aagtcagaaa attgcactaa
     4381 ttgatctagt tcaacaatca aatcttcgcg taatgtacaa caaatgcatc cattcggcat
     4441 cgaaacaagt ttttctccag ttcttttaaa acctttcttg tcgactaata ccccatcaac
     4501 atttacttcc cctatgtcat ttacaatgac agcaattttc tttccgtgat caccgtacag
     4561 aatatgattt agaagtatcg tttttcctga accaagatag ccagttaaga ttgtaatagg
     4621 tatttttttc atagggaacc tctttctata ccttgaattt acactttaag tgcaacacat
     4681 aaaaatgagt cataatcgat atccaactag tataagtttg tcgagaacac tcctagaagg
     4741 aaaataatta tgagccagta tcgtcatctt actatactcg aacgagaaag aatctttctg
     4801 cttcacgaag aaggattttc tattagtata attggcaacg acattcagca tagcccgttt
     4861 actattttac atgaactgag aagaaacaag aaaaaaacta ttcaccttcc aaagcggaaa
     4921 aaaattatgc ttatcgaaga aaaaaatgcg gacgttatca cattctaaat gatccgtgag
     4981 tttggtcaat tgttcgtcat ttatttgtcg attttcagtg gtcgccagag gaaatctcga
     5041 atcgtttgaa gcatgaacat atggactttt caattagttt tatcacgatc taacgtgcaa
     5101 tttgcaatga tcttttcgat acagaacccc tctcacacgg caataaaggc ctgatccgtt
     5161 cattgcacca tcgaggaaaa acaagacata caaaaaatca tgtcgagtct cgtggaaaga
     5221 tttgccttac tcattcgatc catggccgtc caaaatccgc agatgataga agtgattttg
     5281 gtcattggaa agccgatatt gtagctggca aaactggtgg tacttgttta gtgactttga
     5341 cagacagaaa atcatgcttc cttttagcag gaaagaaggt ccccagaaag aaagccgctt
     5401 atgtgagcga gaaaatgatt gaacttttaa gcagtattcc aaatgaggcg gtcaaaacga
     5461 tcactccaga tagaggaaaa gaattctcca atcatttcgc agttacagaa gcattgaata
     5521 atatgccctt ttattttcca gatcctcatg cccttggcaa caatgaacaa attaaaatac
     5581 gaatagactc attcgagaat acttgccaaa atcgaaagag atagctacgg tagataactc
     5641 aacgataaat cagatgattg aaaaactgaa taacagacct agaaagtgtt tagattggag
     5701 aacaccttat gaagtcttct ttgataaagt gttgcgcttg atttgacaat tcaagacata
     5761 ataaaaagca gatcaaatcc aaataaggga aaaatgatct actctggtat tatcaattaa
     5821 ttggccaagc tattatgccg ttttgctaaa cgtctacttc ttccagtatt gaccttgata
     5881 aaacaacaat caggatcccg ttttaatact ctatgaactg tgtcaatact aacatgtaaa
     5941 gttgcagcaa tatcagctaa tgtactccca tttaatctca tttgctttgc aaatcgataa
     6001 cgccgataag catcaatatt cttggaatcc atagacaata atacttttct taccatgtat
     6061 ttcctccaaa aattgaattt taatgagcca tcatttcttg agcaatttca tgtttagaca
     6121 atgaagctgg atagaaagta ggccaattat gcaattcatt atagagtttt tcttgacttt
     6181 ctccaccaaa gaaaatatgg aaatgctctg ccttacttgg tgcaattcca tgatcactaa
     6241 attgaatata tttaaaacga ccagcacctg catcatcagt ttcaaaatta aaacgaacac
     6301 cacggtttcc tttttcataa ttcaaaattt tgtatccaac atatttgtat gtgtattgat
     6361 gatgttcacc gttgactaca aaatcaatgg tgttgtcttt aatgttgata gtatcaatat
     6421 ctgtcttata gccagtagta taataatctt tgtactcggc agctgtcatg tctttattga
     6481 tttttgcttt gtaatcgaaa acttcatcca aaattccatc ttttaacaac ggataaacag
     6541 attgccattc gcctgtataa tcactcaatt cgcgatcctt cacatcggca tctttaaagt
     6601 acccattagc aactgtcttt tcatccttcg cttctttttc tggaactacc tcttcaccag
     6661 caaccatagt tgttttttct aacgctttta gattgtcttc cattactgaa acataatctt
     6721 caccattatc catttgttct tttgtaagac tttcaagagg attcagtact tccaatttta
     6781 tcccagcttc gttggccaaa gtttttgcaa ttttatcatt agcattcttt tcaaagtaaa
     6841 tataattgat cttatttttt ttaacatact cttttaattc agctaatcga ccagaagatg
     6901 gctcttcttc aggacttagt ccagcaatag gaacttgttt caatccataa tccaaagcta
     6961 aatatccaaa ggctgcgtgt tgcgtcacaa aacttttttg tttcgcttct ttaagactag
     7021 tggtatagtc agcatccaac cgtttcagtt tagtcaaata cttttcagca tttgtctcaa
     7081 atacttttgc ttttttcgga tacaatttaa ctaactgatc cttgatattt gaaacttcct
     7141 taattgccat tttgggtgaa acccacgtat gaggatctag ttcatgatgg tgatcttctt
     7201 ctccgtgatc atgatcttct tcttcactac ctggcaataa aatcatgtct tttgttcctt
     7261 ctactacatt aggcccagct ttcttccaac tttctttagc ttttggtacc catgattcca
     7321 tgttttcgtt atgatataca aaaacatcag catcatgagc ttttgccata tccttagctg
     7381 agggctcata gtcatgaggt tctgatcccg ctggaaccat taaatccacg ttcgcttcat
     7441 cgccgacgat gtttttggtg aaatcataca ttggatagaa tgttgttaca atttctaatt
     7501 ttccatcttt atcattcgat acttttgcat cattagtgct ggagcaagct cccaataatc
     7561 ctagagctgc tatcccaata agtcctacac tgattttctt cattcttttt ccttccttaa
     7621 ttatgctaat ttcttcattt tatttcgttt atgttctttg ataattttta atgccctttt
     7681 tctagtttta atattcgggc ttttaagtcg tcgatatgca ctagatatat ccgttttctc
     7741 cattagaaat tacccccaat cactacttat cattatttgc tttccgccag taatttatca
     7801 aaactctgtt tgatcctttc ttcatccaac atttttccga ttagaataat agtggtttgg
     7861 tctttttgtc gagtggtgta ttgaaaatta acctgttcgt ttacctcttg catggcaact
     7921 ataaaatcct gctcttgtaa agcaatcaat cctttaaaac gatacaggct cccttggttg
     7981 gttaaaatca gccagtcaat ccattggagt aaaaaatctt tttttatgtc ttgatctgaa
     8041 gttaagagaa ttgatttaaa ctcatgtact tgatggtgtt gatgatagta tttgccatta
     8101 tagtgatttt ctgatgaatc actttcaaaa actaagggat gattaaattt tttcaaatta
     8161 taaaaatcct ttttttgaat cgtttcacta tgtgtgaaca ttcgaatatc ggataaaggg
     8221 ttaatacttt tgatttctct ctgtaatttt tttagtgcag ataaattcaa gttttgtttt
     8281 ctagacacaa aaatgcgatc tgccatgaca agttgcttaa gggcttgtgg ttctctttca
     8341 ataagttcaa aactgtcact atcaataaca gtcaataaac tatcgatata tactcttcct
     8401 cttagttgag gcgctgttaa aagtgtttga atgattggtt ggggatctgc tatacctgta
     8461 gtttcaatga tgatttgtga aattggttgt tgatgttctt ctattgtttc cagtaaagaa
     8521 atcaatattg ctaataaatc actgcgaaga ctgcaacaca tacatccatt atttagttgt
     8581 aacacccgtt cctcgctatg aactaataat ttgtgatcaa tcccaacttc accaaactca
     8641 ttttcaatta taataatttc ttccctgggg tagggtgaga cttgcaaggc ttgattgatt
     8701 agcgttgttt ttccagcccc taaaaaccca gaaacgattg taactggtat gcccattttg
     8761 tcaccttttt accacttagc tatttcactt ctttaaaaat tgcccgtctg cgcaattttg
     8821 gcgaatactt ttttaattct aaccgttcag ggttattccg tttatttttg ctggtgagat
     8881 acagacgttc tccagtttcg acacattcca aaataatatt ttgacgcata aatcttcctc
     8941 cttcaatact tatcgatctt caagatatgc ttgaatttgt tgtgcatctt caaaagttaa
     9001 ctcgccagat tccaattgtg aaactaaatt ttctttcata tcaacaggta tctcccaata
     9061 accatcgata cattgaaata actcctcgtc atactcttca aaaattggat agattcttaa
     9121 aaaatcatca aatgataagc cttggtcctc atagtaatct ttgactaatc cattttcaat
     9181 tgcctgttgg gtcggtaaac ccgtttcagt aataattcct ctctctacca tcgaacgata
     9241 aaaagtgcat ttatctagcg attgctgggt cgtttcaaga taatacttta atccttcttc
     9301 aactaaaggt gggtaaataa tttccatttt gattccctcc taaaagttat atattaaact
     9361 attttgaagc tcctttaatt actttctttc cattatagta cccttttaaa gatacatgat
     9421 ggctacgtct ataatcacca ttcgcttcgt caaatgaaat ttctggtttt actacagttt
     9481 gatgagttct tcttaaccgt ttcttcgcct tagatgtttt tcttgctggt actgccatga
     9541 tatttcctcc ttttaccaac ttgctttttt tactccagga atcaaccctt gatgggcaag
     9601 ttctctaaac ttaatacggg acataccgaa ctttcgcata taccctcttg gacgaccatc
     9661 cgtctgatca cgaagcctca gacgattcgg atttgaatct cttggcaatt tagcgagagc
     9721 ggtgcgatct cctgcggctt ttaattcctg acgtttatct gcatactttt caatcatcgc
     9781 catttgtttc tttgctttag caattttcga cttttttgcc atataccttt atactccttt
     9841 tattttgatt tagacacctc aaatcgtaat cgttacgttt tgacactgat actttaccaa
     9901 caagagttgc tcttgtcaac aataaataac atgaaataaa actcatatga atagataact
     9961 atttgaaaac gaatttatcg gtacatagag ataatcaaat catttaaaag aatatgcagt
    10021 ttaggctctg aaaaataaga attcactttt attagtccaa tcaaacacaa ataccgtttt
    10081 aagtagcttt tcctctactt aaaacggcaa tatgaaagaa actataactt tttattcaat
    10141 cttttatgtt taactggttt atcgccaatt ttttaaaact ttgattattc cttatattat
    10201 aagaatagac attaagtgaa ctaattcaat catactgatt ctttcgtgta ttcaaaatta
    10261 gatgctgttt aataaaatta ttctttttga aaaagaatta taactatatc ccaaaaattg
    10321 taggttaatt taaagcctat ttcatcatcg tgattgagct tctttttcat agacgaattt
    10381 ctcagatagt ttagttttct ttatgagatt tttgtatatt tctgaccttc gatacaactc
    10441 attatgagta ccttgatcaa ctagttttcc ttgatccatc actacaattt tatttacctt
    10501 ttcaatagac ttcaagcgat gcgatataat tagtactgtc ttatcttgta ttaaacgatt
    10561 taaactttcc tgaatacgtt tttcattttc cgcatctaaa ctggcagtaa tttcatctaa
    10621 aattaaaatt ggcgcatttt tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc
    10681 tccagaaagt gaacttccgt tttctccgac caatgtatga taaccgtcag gtagtttttc
    10741 aataaagtct tcgcagttag ctaaacgagc tgcttttttc acttcctcat ctgacgcttg
    10801 actatttcca atacgtatat tttccaaaat acttgtatta aacaaagtta cctcttgaaa
    10861 aacaatagcg attttgctaa acaaagaagc tggagataca cgtttaatat catacccatc
    10921 aattaaaata cagccttcat cataatcata cagtctagac actaatttca agatactagt
    10981 tttcccggaa ccactagcac ctactaacgc agtcacttct ccttgtttag ccgtaaaaga
    11041 gatatgatct aaaattgggg tattgttatc atatgagaaa gagacatctc gcaactcaat
    11101 gtcaaaagat ttcagaggac tatctgatcc ctcttggata ctggtttcct tcatagctct
    11161 aattcgctgt attttaggag ctaaataaaa tatttcaagc actgcttctt tcatagaatc
    11221 gaaggaatcc tttattttta ttgcggctag taagtaacca acgacgtaga gtatagtcac
    11281 ttcacccgtt aacagtaagt gaacacctac tagaatgact actgctaaac taacatatga
    11341 gaaaatagag gataaggcca tgactgaaaa agtacttaat tctaccttta aatgaatcct
    11401 ttcactctct tccatttttt tgaaaagtct gtcttgaact tttttagata gattaaagct
    11461 attaatctct tgctgcaatt ctatagtttc ttgaaattct tccgagttct ctctcaaagt
    11521 atcgtaatat ttagtattgg ctttcgtttg ggatttcttt gataacaaga ttaagacaaa
    11581 actaaataac gttggcaaaa taacagctaa tcccattttg acattaccaa ccaaaagcat
    11641 cactgaaata aaagggaaaa acagagccat accgccggct ttaggtattg catgactcat
    11701 cgcatgctca ataccttcaa cgtcagacat gatagtttga gataaatctg ataaattatg
    11761 ttttgaaaaa taggataatg gtaaatttga caaatcgtct gcaatttgca ctcttaaatg
    11821 cgcactttct ttataagtac tattatataa gttttcatac tccctatcta acaaccaata
    11881 catgaaaagc aaagtaacaa acgatacggc aaaataaagc caacggggct ttgtattttc
    11941 taaaactaat tcgtctatca gcatcattat aatgaatgct ggtaccatat taatgcagta
    12001 tacaaaaaaa ctagaaatag aagctttagt taaagcttct gcgccctgat cacttaaagc
    12061 aaatttgttt ttataaaaac ttttcattgt caagcctcca atcatttgca ttctcataat
    12121 tagtgaccaa ttgtttatac acttgatgtt tagccattaa ttcattatga tttccacgtt
    12181 ctactatttt cccttctgac aagacaagaa tttcatctac agatttaatg ctggataatc
    12241 gatgagctat cataataact gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt
    12301 catactcatt atctggatca atagatgcac tggcttcatc cataataaca atctttgcat
    12361 cttttaaaat tgctctggca atagctatac gttgtttttc tcctcctgac agataaactc
    12421 ctttactacc aattaaggta tgctctcttt cgggaaattt ttctaaaatc gattcgcaac
    12481 cggctaatct tagcgcgtca aatactttac ttgctggcgc ttgaggattt gctatagaaa
    12541 cgttatcgta aatactctga ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt
    12601 taataattgt gtcttttgaa tagtgattta aaggcttacc accaatttta atctgtccct
    12661 tatccacatt gtagaaacct gaaattagtt tagctatcgt actctttccg cttcctgatt
    12721 ttcctactaa tgcataaatt cttttttgtt ctaaaactaa gttgaaattt tctaaaacac
    12781 actcttcttt gtatgaaaag ctgacatttt caaatgcaat gtcacaattt ttaatctgtg
    12841 tctcgttccc atattttagt tggtcttgct tcatttctaa ataaagggag tctagtgttt
    12901 cgactgcata tcttgcttca aacaagtgca tacttacata cattattctc atgaaagaaa
    12961 caaaaataat tccacttaaa aaaaacaaca tcaacaagtc aacagctaat atcttgggag
    13021 tggtaagaga agttataaaa aaggttatag gaactatcaa aatgcaaact atgccaaaaa
    13081 atagccattg aaaaagaaca aaaggtgttt tcccactcaa agaatactta tatgcaaact
    13141 tagcataatc gttaattgct ttattcaagg ctttaaatga tgttacttta gcaccaaata
    13201 tctttataac ttgaattccg cggatatatt ctaccgtttc agagcttaac acatctaacg
    13261 attcttgata tttttgaata aaatttgtat tctctgtcat tttctttaac aagaatacac
    13321 caattactaa catggcgatt agaaccaatc caattttaaa gttaatgaaa aagcctaagg
    13381 caactgataa aacaggtgtt actatggctt gagcactatc tggaatcaag tgagcaattg
    13441 ccatatgtgt tttagccgcg ttgtcatcaa ttgtctttcg aatatatccc gaagaattta
    13501 aatcaaaaaa acgaaaactt gcttctgtta aaccgctaat tccctttttt ctcaaattag
    13561 tctctaagcg aaacccaagg atatgagaaa ataagccaga aaaaatataa aataaagctc
    13621 ctaaagttaa ccaagcaaca gtttgaaccg cataactctt agctaaatat tcatctccta
    13681 caataattag agactgaaga aatttgtaaa tactgtaata tccatagact gttaatacga
    13741 cagaaaaatc agaaaataaa gtggctaacc atccgtagta tttcatttta gggacataac
    13801 taaataattt tttgtatact ttcatttttt cctcccccgc attcaatttc ttctcttatt
    13861 cgtttacaac tgttaatgct ttccgtaata gcgtaaatta gagatgtaca aacagataaa
    13921 aggtaaacta cgttctattt tttgtatttt tttaagactt ttcttccaat caatacacca
    13981 atcatagata aacttgctac aactaacact gaaatgattc cccatatacc taccgcatag
    14041 tggatcattg catccattgt tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa
    14101 cgatctgtaa agaaccatat aggaatccac tgtccggcag cactgcctaa ttgcattaga
    14161 gcccaagacg caacttgagg aattgctcga tcatatccaa atacagatag aataatatca
    14221 gctaagatcg ccatactaat cgaactgata aaccaaggta agtacgcccc gcccattaac
    14281 gtaaaaaata acaataaaac aattgtgtaa atagtaaaaa ttccttttct agggtagtta
    14341 tagctaatat aaacaaatat gattccagat aataatccaa aaatcccggg ggatatgctg
    14401 tgaccaaacg ccccaccaat actactaacc attattgaaa aaaagtaaat tactagggaa
    14461 ataactgtag ctaaaccaat aaaaatataa tcttttgttt tcatatatat tctcctcaat
    14521 tctatttcgt tatgtcatta aaaatctgtt ctatcaaagt tgaatcttct actctaagtt
    14581 ttatatcttt ttttattcca ccatttttta aataaataat tctattgcat gttttataaa
    14641 gaaattccgg atcatgagta gctataacta ctgcaccctt ttcttttaag tctttaataa
    14701 ggtcagcaat cctttccata ttaatataat ccaacccact tgtaggttca tcaaatagaa
    14761 ataaattagt ttttgacaat gaagctatag aaaccaataa tctttgtttt tctccaccgg
    14821 ataaatcaaa cggtaattga tctttcattt tataaagttc catttttttt aaaatttttt
    14881 cttgctgatc tttactaact atttcattcc ccagggctaa ctcgctcctc actgattctg
    14941 tgaaaaattg gtaatccatt tcttgcatta ctaaaaacgg cgatgacaaa ttttttttat
    15001 tttcgattat ctttcctttg gtagggcgaa taatcttcat gagtattctt agtaaggttg
    15061 ttttccccac accgtttttc cctaccaaac caataacatc tccaactttg acatcaaaat
    15121 ttatgtcttt tagaatattt tcataagtaa tttcttcaac ttttaataca tctgcactta
    15181 ccttttcgat atagggaact tcaatcg
//
LOCUS       DASJTC010000068        15002 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.068, whole genome shotgun sequence.
ACCESSION   DASJTC010000068 DASJTC010000000
VERSION     DASJTC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.068"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(524..1090)
                     /locus_tag="U4B19_001964"
     CDS             complement(524..1090)
                     /locus_tag="U4B19_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEN2077692.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1090..2046)
                     /locus_tag="U4B19_001965"
     CDS             complement(1090..2046)
                     /locus_tag="U4B19_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEN2077693.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2046..2702)
                     /locus_tag="U4B19_001966"
     CDS             complement(2046..2702)
                     /locus_tag="U4B19_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEN2077694.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3072..5090
                     /locus_tag="U4B19_001967"
     CDS             3072..5090
                     /locus_tag="U4B19_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2077695.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5160..5366
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5517..7931
                     /locus_tag="U4B19_001968"
     CDS             5517..7931
                     /locus_tag="U4B19_001968"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEN2077696.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8279..9832
                     /locus_tag="U4B19_001969"
     CDS             8279..9832
                     /locus_tag="U4B19_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEN2077697.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10213..11997)
                     /locus_tag="U4B19_001970"
     CDS             complement(10213..11997)
                     /locus_tag="U4B19_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077698.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12012..13733)
                     /locus_tag="U4B19_001971"
     CDS             complement(12012..13733)
                     /locus_tag="U4B19_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077699.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13833..14594
                     /locus_tag="U4B19_001972"
     CDS             13833..14594
                     /locus_tag="U4B19_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2077700.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4887 a         2750 c         2965 g         4400 t
ORIGIN      
        1 ccatctggaa tttatctttt ttactgcttt gatcataaaa gtctgcatat tcatcactga
       61 tattttttct aataaattct tccatcataa aaatcacctc aaaacgatta tacgaactta
      121 cgttcttgtt gtaaagcgat atttgagggg caaaaaaggg gcataatttg actgatatta
      181 agttagctat tgatatatga atgacataca aaaatataaa atcttttaaa aatgcttata
      241 tatcaatgtt tttgccattc ttttatatat ctgttatatg tcaaaaaaat gtgttcaatt
      301 tcaatttgta tttgaataaa gaggttgtga aaaagctgtc ctgcatcaag taataagaac
      361 agccaatcaa aaacagcttc tcatgttttt gatttggttg gacttattac cgcagatata
      421 agcttttgag caccgtgaat aaagaggtta tgaaatcaac gctctaacct gagtattaga
      481 gaaaaaaacg caaaacatat catttatgtt ttgcgttttt tatttatctt tttttctcaa
      541 tacaatataa aataggcggc tggttctttt ggttgataaa ccgataattc agcacattat
      601 atgtttcctg tggcagtttg gaacaaaaag aatcaaccat atccagttct ttttctccac
      661 cttcatgacc atagtagacg acaaggatca atcgtccgcg aggcacaagt cgtttcaata
      721 tttcttccat agctgtgcgt gtagtctctg gcatagtgat gattgcttta tcactttttg
      781 ggagataccc tagattgaaa atccctgctt tgagattttg ttgttcagct atcacttctc
      841 ctaacgtttc gtggccttgt agaaacaatg agacacgttc ttctaactga cgctgttcta
      901 gcctttcacg agtagcatcg attgcttgtt gctggatatc aaaactatag acatgaccag
      961 tttttccgat gtgttctgct agaaaaaccg tatcgtggcc attgcccatc gttgcatcta
     1021 cgacgtgatc ccctggttgc aagatttctt gcagtaatgt atggctgaaa tgcaaagcag
     1081 tttgcaacat cagacgctca cctttttccg tacatcatat tttccttgga agctatctct
     1141 gcgaagaagt tcttcatcaa ttgcattcaa gacttcccat tttttcaagc tccacattgg
     1201 tccaatcaag gaatcccacg gcgcatcccc tgtcaaacga tggatgatga tctcttttgg
     1261 tatcatttcc agctggtcgc aaatgacatt gacatagtca ggacggctca ttaattgaag
     1321 gcgaccttca tgatagtccc gcaacattct cgttttgcgc atcagatgca tcaaatggag
     1381 cttgattcct tggatatctg aatctaaaat ggtgcgtctg acattttctt tcatcatttc
     1441 taaactttcg cctggtaatc cgttgatcaa atgcgtacaa acacggatat tatgtttgcg
     1501 taatttagcc acgccatcta aataagtttg ataatcatgt gctcggttga tcagttttga
     1561 agtttcttca aaagttgttt gcaatccaag ttctacccat aaatacatcc gctcgttcag
     1621 ttcagcaagg taatcaacta cttcatctgg cagacaatcc ggtcgtgtac cgactgataa
     1681 tcctacgaca cctggtaaat tgactacttg ctcaaatcgt tctttgatta tttcaactgg
     1741 cgcatgggtg tttgtaaaat tttggaaata aacgatgtat tgttggacat gaggccattt
     1801 ttgatgcatc atgtcgattt ctttctggaa ttggattgga agagggtctt caggtgcaac
     1861 gatcatgtct cccgaaccag atacactgca aaaagtacaa ccgccatggg caacagtccc
     1921 atctcgattc ggacagtcaa atcctccatc gatcggcacc ttgaaaattt tcccgccaaa
     1981 ctcatttcgt aaagcgtaat tccaagaatg atatctttta tttccttctg aataaggaaa
     2041 agtcattaaa agtccttctt tctgtaagag ttatctattt gttttctttt ttctctatac
     2101 actggataag tcaggcacag ccaagaaaga ctgagtaagt aaccgccgat gatatcagta
     2161 ggaaaatgaa cgcccagata aatgcggctt atcccgacac ttactataat aaacatcatc
     2221 agcaattgaa gcaatgtgcg taaggttctt ttttgaacaa aagctggaag cagaaatagt
     2281 aaagaaccgt aaagaatcat actgcctgct gcatgtccac tagggaaact gtaacttgtt
     2341 tcaactacta agtgcgccaa tgaaggacgc tctcgcatga aaaatagttt gatcaccggg
     2401 ttgatgattc cagcaattac aataaatcca gaaacgaacc aaaatgtttc tgcccttttt
     2461 ttcttatacc acatccataa agccgcacaa gcaaataaaa tgatgacggt aagaggatta
     2521 ccgaatttag tcataaagag ttggtagctg ttcagatcag gataagaagc tctgacaatg
     2581 cctgtaaacc actgatcgaa accagaaagc cacgacagat ggaactttac gatccagccc
     2641 aaaatgaaaa acaggacaag aaagaagcta ccagccacct gaaatgatgt tttatatttc
     2701 atacaattcc tcctttgaag caatgactta ttatagcatt tttggtccag cgaaaaaaac
     2761 ggtaaagaga taaaaccaat gtatgattcc tctttgaata accaatattc ttataaaaaa
     2821 aataactgta atcggaaaga cattttttgg taatgcaaat gaaatgttag ggaaataaaa
     2881 tcagtgtttt tcgaaatcct tttaaacaag aacgtagacg taggaagaac ttgaaaatga
     2941 acagaagttg ttcattacgg taaattacaa tatgattaca aaaaacaaca aagctcttaa
     3001 ataactttta ataaatatta agagaagtta taattattaa taatagataa attgaagttg
     3061 gaggatagaa gatggaaaac attgcaagaa aagaacggag acgtttagaa cagaccaaac
     3121 gttttcgaaa agcaaaacga ggggcagcaa tcgtgggaac tgcgatggta ggctgttctg
     3181 tcgctgcccc attagtacag ccggttcctg ttcaagcgga tgaaacacct gttcagttcg
     3241 gcgcaagaat caatacagcg gcatttatca cagaaattgc tacttatgct caaccaatcg
     3301 ctcaagccaa cgatctatat gcatcagtga tgatcgcaca agcagttgta gaaagcggtt
     3361 ggggcagtag tgcgttatct caagcacctt attataatct ttttgggata aaaggcagct
     3421 atcaaggaca aaccgtctac atggatacgc ttgaattttt aaataatcaa tgggtaaata
     3481 aaaaggagcc tttccgacaa tatccttctt ttgctgaatc tttcaatgac aatgcctatg
     3541 tacttagaaa tacttcattt ggcaacggtt attactatgc cggtacttgg aagagcaaca
     3601 cgaaatccta tacagatgcg acagcttgtt tgacagggcg ttatgcgact gatcctggtt
     3661 acgcaggcaa attaaataat atcatcgcaa cttacggatt gacccaatat gatacaccag
     3721 ctagcggaaa cgcaggcggt ggagcgacta caggtggcaa taatactgga agtgcaggta
     3781 gtactggcgg ttcaagcaat tctggtaata caaactcagg ttcagcaact tcgaccactt
     3841 atactgtcca atctggtgat tctgtttggg gaatctcaaa taaattcggt atcacaatgg
     3901 atcaattgat ccaatggaac aacatccaaa acaatttcat ttatccagga caaaaactga
     3961 caatcaaagg tagccaggaa aatggctctt cgacgaataa ttccggtaac aataccaatt
     4021 cttcaggaaa tgctggtaca tcaaacggag gacaaacaac tggtgcgaaa tacactgttc
     4081 aatctggtga ttctgtttgg aagatctcaa atgatcacgg aattacgatg gctcaattga
     4141 ttgaatggaa caatatcaag aacaatttcg tttatccagg acaacagcta atcgtatcaa
     4201 atggcggttc ttctaatgct ggagcaaata ctggctcaaa tactgggaat acaagtggct
     4261 ctagttcgtc cagctcaagt tcttctaata cagcttcagg aacgaaatat acagttaaag
     4321 ccggagactc tgtttggagc gtctcaaata aatacggaat tacgatggct cagctaatcc
     4381 aatggaacaa tatccaaaat aactttatct acccaggaca acaactgact gtttcaaaca
     4441 gtggatcaac aaacagcacg tcaggtaata caggttcgtc aaccaataca aataacagca
     4501 gttcagttaa tactggcagc agttcgtctc aaggcactta taccgtgaaa gcaggtgaat
     4561 ctgtttggag cgtggcaaat aaaaacggca tcacgatgga tcagttaatc gaatggaata
     4621 atatcaaaaa caacttcatt tatccaggac agcaattgat cgttaaaggt ggaacttcgg
     4681 caaatacaaa tacaggttca actacttctg cagcgaaacc aaatacaccg aatacgtctg
     4741 ctacgacaag tacatcatcc actggtaata cgatgtatac cgtgaaagcg ggcgagtcag
     4801 tttggagcgt agcgaacaaa catcatatca cgatggatca gttaatcgaa tggaataata
     4861 tcaaaaataa tttcatctat ccaggacagc aagtgattgt gaagaaaggt tcatctcaat
     4921 caacaaacca acaatcagtt actggttcga aaacctatac tgtgaaatct ggagaatctg
     4981 tttggagtgt tgctgacagt catggaatta caatggctca actaatcgaa tggaacaata
     5041 ttaagaacaa ctttatttat ccaggtcaaa cgttgattgt agctaaataa aatagctgtt
     5101 cgaagaaata acgattgaat tccacaaaaa tatttgttac tataaggcta cttaaatatg
     5161 acaacacttg aaaaggaagt agtaaaaaga ctgaagtttc agagagcgca tggctggtga
     5221 gaatgtgcac ggatgctttt gaactcgcct ttgagttgcc cttgctgaat acccgagtaa
     5281 gcaagcggcg gtgacggccg ttacaacgtt cgaggccttt tcactaaggt aaaattaggt
     5341 ggtaccgcgt agattacgtc ctataaaaag agcaatcttt ttataggatt tttttatttc
     5401 ttttgtgaag tttgagaaaa caaagcaaaa ggaaataaaa ataaacagat tctgccggtt
     5461 ttttcgttat atttataata gaaacaagtg ttttcaatag aacaggagga cagcaaatga
     5521 gttacaatca taaagaaatt gagaaaaaat ggcaaaaata ctgggcaaaa acgaatactt
     5581 tcaacaccca tgatgatcct gaaaagccaa aattttacgc actggatatg tttccctatc
     5641 catccggtca agggctacat gtagggcatc ctgaaggtta tacggcaacg gatatccttt
     5701 cacgtgtgaa aagaagtcaa ggatacaacg tgctgcatcc aatggggtgg gatgcattcg
     5761 gtctgcctgc tgaacaatat gcgctagata caggaaacga tccagctgag tttacccaaa
     5821 aaaatatcga aacattccga cgccaaatca attctttagg atttagttat gactggaatc
     5881 gcgaagtgaa tacaactgat ccggagtatt ataaatggac acaatggatc tttacgaagc
     5941 tttatgaaaa aggtctagct tatgaagcag aagtagcggt aaactgggta cctgaattag
     6001 gaacagttat ttcaaatgaa gaagtgatcg atgggaaaag tgaacgtggt ggctatgatg
     6061 tagtcagaaa accaatgcgc caatggatgc tgaaaatcac tgcttatgct gatcgcttat
     6121 tggatgatct agaattagtt gattggccag aaagcattaa agaaatgcaa agaaattgga
     6181 tcggtcgttc agtaggagca aatattgaat ttaaagtggc tggtacggat aaatcctata
     6241 ccgtatttac gacacgacca gatactctat tcggtgcaac ttactcggtt cttgcacctg
     6301 aactagatct tgttagagaa attacgaccc ctgaacaaaa agctgctgta gaagcttata
     6361 tcgaagagac agctaaaaaa tcagatctaa agagaacgga tcttgcaaaa gaaaaaacag
     6421 gagtatttac tggtgcttat gcaatcaatc cagttaatgg caaggaaatc ccaatctgga
     6481 tcgccgatta tgtattatct tcatacggca caggagccat catggctgtt ccagcacatg
     6541 atgaacgtga ctttgaattc gctcaaactt tcggactaga aattcttcca gttatcgaag
     6601 gtggagatgt tgaaaaagct gcttacacag aagatggtac gcacatcaac tctgaatttc
     6661 tagacggcat gaacaaacaa gaagcaatcg ataaaatgaa tgaatggcta gaagaaaatg
     6721 gcgtagggaa aaaagaagtc agctatcgct tacgtgactg gctattctcc cgtcaacgtt
     6781 actggggtga accaatccca atcatccatt gggaggacgg aacagtcaca gctgtcccag
     6841 aagaagagct accattacgg ttgcctaaga caaaaaacat caagccaagc ggtactggag
     6901 aatctccatt agcaaatatc gaggagtggg tcaatgtcgt tgatcctgta acaggtaaaa
     6961 aaggccgtcg cgaaacgaat acaatgccac aatgggcagg aagctcatgg tattacctgc
     7021 gttatatcga ccctcataac aaaaaagaac tagcagatta tgaaaagttg aagcgttggc
     7081 taccagttga catctacatt ggtggagcag aacatgcggt acttcattta ctatatgcac
     7141 gattctggca taaattctta tacgatatcg gtgttgttcc tacaaaagaa ccattccaaa
     7201 aactatacaa tcaaggaatg atcttaggcg aaaacaacga aaaaatgtca aaatctcgtg
     7261 gtaatgtagt caatcctgat gatgtggtcg aaacttacgg tgcagataca ctgcgcatgt
     7321 acgaaatgtt catggggcca ttagatgctt cgattgcttg gagcgaaaat ggcttagaag
     7381 gaagccgtaa attcttggat cgtgtatggc gtttgatcgt tgatgagaac aataaaatgc
     7441 gtgaccgtat cactacattg aatgatggca agcttgataa agtgtatcat caaacagtga
     7501 aaaaagtgac agaagactat gaaaatcttc atttcaatac agcaatctcc caattgatgg
     7561 tctttatcaa tgaagcctat aaagtggacg ctctgcctta tgaatatatc gaaggatttg
     7621 ttcaactact cgcaccgatt gctcctcaca tgggtgaaga actatgggca atccttggaa
     7681 atgatggtgg tatttcttac gcaccatggc caacatacga tgaagcagca cttgttgaag
     7741 acgaagtaga agttgttttc caagtaaacg gtaaagttcg tgcgaaaagc aatgttcccc
     7801 gagacttagg aaaagatgag ctagaaaaag cagcattagc caatgaaata gtgcaagaat
     7861 atattgaagg caaaacagtc agaaaagtta ttgcggtacc aaacaagtta gtcaatatcg
     7921 ttgctaatta attggctctt tgtcaataag gactgatgag ttgtgcaaaa tcaaatctgg
     7981 gccagaatga acttcattct gtctcagatt ttttgttatt tcaaattgat taattgattg
     8041 atcaaaaagg ttttgatttt catgttctca aacacgttca atgtcttttt cttctgaatg
     8101 gtcttgtaag agatctctgt aatcatcaaa tttttgtcta ttaatcttag tattttttga
     8161 tgtataaact acctgaaatt gagagtacat accaaattca taaatttttt attagtggga
     8221 ctagtgattg tatggatttt gacgcataat gaatgaagta tgagaaaagg agagacaaat
     8281 ggatcaagaa ctatttatta tcgaagtatt atcagctaaa caaaaaatta tcgagaaagc
     8341 gaaaaaaaga gcgaaaagaa taatgttcaa gcttttaagc ttagtatttt tctctggttt
     8401 gctttttttc ggagctgtat cgttcctata tcgccatatg agtgcagtag tccgttcagc
     8461 tgaagggatg caaaaattaa ataatttgta tcacttttcg tggggagttg gcggaataac
     8521 aattgttgtg cttattgttt ttcttctact tatttggctc cgttaccatc aattgtcgcg
     8581 tcatttcgat caatggctta gtcaaagagc acaacagttt ttacataata aaccaacttc
     8641 tcaagatgat gactatcttt attttgagaa gacttcgacg agtcaagctt ttcggataca
     8701 aaaaagtgat tgccagttat tgctgagatc acttgatgat ttacctatct acttaggcga
     8761 aagcaaatca aaattcggaa tgaacacatt ccatattttt ttactagaaa acgaacccag
     8821 atgggaaaaa agccagtctt cgcctggtgg atacctgaca actaagatga gtacattagt
     8881 cactggtttt ttcctcattc ttttagctgg aacaatctat tatgggagat acactcagcc
     8941 aaaaatgtct tctcatataa atgctgcttc tcacatgaca atgaactcaa ctaacgatac
     9001 gcatatacaa aagacaaatg aagggttgtt agctgatcct aagcaaaagg taaatttgac
     9061 aacacaaaag ataaatgatc tcaaacttga tacagagacg cactctctct atatgacaac
     9121 agatagtggt gctaattggg tatttgtgcc gattgattcg caatggttgc gttttggcga
     9181 atacaccctg acaacggggc tgattcctga aggttattgg atggacaaca catatgatat
     9241 ttccgccgat ttctcatggt ttatctattc ttctggggat gatgtacgtc tgctgacttc
     9301 aagagataat ggtaaaacat ggcaaacgtc tattatcacc caatcagttc agccgatacg
     9361 ttatcgaaaa gcggtatttt atggcaacga tggtctattt atctgtacat cagccagtgg
     9421 gatgtctgca gaaactttgt acatttacac tactatggat ggcggaatca cttggataaa
     9481 aagtggaagt acaatgatca atcagccagt acaaaatgtc agcttcgtta caacttcttt
     9541 aggttttgtt tcgacacgtg aaagtttgtt ttatacaaac aacggaggac gctcattcaa
     9601 agaatcagtt ctgtcgattc cggaaaacta tcagacaggg gggatagatc tttttcaatc
     9661 accaaatgaa gttactcaaa taagcgcgaa tacgttagag acaaaatttt atttgttgaa
     9721 aaaaggacca atcgatgcag ggaaaatgtt tgcctgcctc ttccgttcaa ctgataacgg
     9781 ggaaacatgg caattcgttc agcaactatc gcaagtagaa caaggagatt aaagaagaga
     9841 gattgtgagc ctgacgttca gcaccgagtc acaagaaaca aattcaaaaa acagcttctc
     9901 atatttgttg aatagtgtaa ctcgtgattc ggagttgaca agagcaacaa gaagcgattt
     9961 tgaagtatag cttctcgtat tttacaagat cgtgtcttat tgccgatagg ttgtctcttg
    10021 aacaccgttt attcgtaaaa agggattgtg acaagtcttt tgtcactact taccgaacga
    10081 aactgaagga gctatccaaa aagtatcatt agtattattc tttgtgtttc tagtggcacc
    10141 actagtacat ttaaaaaagc tatcgatcat ggactaatca tacatgatcg atagcttttt
    10201 agataatgtt tgttacgcta tctgaaattg actattataa agactggcat aaagcgttgg
    10261 tgattggagt agttcagaat gtgttccttt ttcgacgata tcgccattct tcattacaag
    10321 gatcatgtct gcattttcaa tcgttgacaa acggtgtgca atcacaaaac ttgtcctatc
    10381 ttttgtcaca gcttccattg cttgctgaat caatgcttct gttcgtgtat ctacgctaga
    10441 agttgcttca tctaaaataa cgacaggagg attcgctaga atgactcgag caattgtcaa
    10501 taattgttgc tgtccttgag ataacgtgcc gttttcgcta gaaatgatcg tatcatatcc
    10561 gtctggcagt gttcgaataa agtgatcaca ttgtgcgatt tcagccgctt cgataatttg
    10621 ttctcttgtg gcatccattt tcccgtaggc gatattatca gctaccgttc cttcaaacag
    10681 ccaggtattt tgtaaaacca tcccaaaaga acgacgtagt tctccacgtg aaagcttggt
    10741 aatatcaaga ccgtcgaatt cgatctttcc tttattcagt tcataaaaac gcatcagcag
    10801 attgactaaa gtcgtttttc ctgcaccagt tggaccaacg atcgctaccg tttgttttgg
    10861 ctttactgaa aaatcgactt gattcatcaa tattttatct gatgtgtaac caaactgtac
    10921 atttttgaaa gctatttttc ctttcggttg ttctaaaagt cttgcatctg gaaggtctgg
    10981 aatttcatct gtttcatcta atatctcaaa tatcctatcg attgctgcca ttgcggattg
    11041 gatcgagttg atcacataag aagcggtaga aattggttca gaaatctgat tgatgtactg
    11101 aagataagct tgtaagaagc cgatcgtcac accacctgat agaacaagca tagcaccgaa
    11161 taccgcactt atgatgaagg cgagttgatt aatgaaacga atggccggat agatagcaca
    11221 gttcataaat tgagcacgtt taaaagcgtt gtattgcttt ttatttactt cgtgtatagc
    11281 agcttctgca tgagcttgct gattgaatgt tttgaccact agattaccag ctaagatttc
    11341 ttccattcga ttatttaaaa cacccagttc atactgattc tgttcagaaa tctgtttgtt
    11401 tttgttcgca atcttagttg taacaattga actgccaacg atcagcaata agacgagcaa
    11461 agtaagcttg ctttcgatat aaaacagcat cagaccagca gcagctattg ttacgacaga
    11521 agtaaaaaat tgattgatac ctgtcaataa cacttgagaa agttgattga gaccggtagt
    11581 tgtccgacta atgatgtcac ctacttgatg attgtcaaaa aacgacatcg gtaatgtttt
    11641 gaattttttt gtgatttctt ttcgcaccct caatgtgatc ttttcgctta acgctgccat
    11701 tgtccgttcc tgaataaatg aagtgatgct gcttaagact gaacagccga tcagcaataa
    11761 gacagggaac aacaatgctt gtttgatatc actgaaagaa agctgtccaa tacctgtttt
    11821 tcttatttct gataagagat catcgatccc gattcccatg atcaagggca tggctacgac
    11881 taataggttc ccgaataaac tgcatagaaa caatccgtaa aaatatttat gttcaggttt
    11941 gatcattttc caaaaacgta tcaaagcacg cgtgttcaac gtttttttct tttccatcgt
    12001 atcccttctt tctatattcc ttgtgatttt gcaaactcct gataagtttg gcaattagac
    12061 agtagttctt catgagtacc ttgtccaacg atttttcctt catctaaaac aatgatgtta
    12121 tctgcttgtt ggattgtact gaggcgttga gcaacaatca cgagagtttt ctccgacatg
    12181 tgtttatgaa gggctgctct taatgctgca tctgttttat agtctaaagc ggagaaacta
    12241 tcatcgaata catagatctc tgctggtttg atcaacgctc gagcaataca tatccgctgt
    12301 ttctggcccc ctgaataatt agaaccgcct tgagcaacaa agctgtccaa tccgtcagga
    12361 agggtgtcca ctagttcttc taattgagcg atcttgatcg ctttagacaa ctcttctgtt
    12421 gaggcatatt catttcccat ctgcaagttg cttaaaattg tcccgctgaa taagaaagcc
    12481 ttctgaggga cataactgat gtgggagcgg atctgttctt gagtcatttg agtaagagga
    12541 ataccaccaa gggttacttt tcctatcgta gggtcactaa gtctcaagag aagtttcgcc
    12601 aaactacttt ttccagatcc ggtcccgcca acgatcgctg ttgtttttcc tttaggtatt
    12661 gtgaaatcaa tatcttctag aactggatca gagatttcag tatatttaaa tgtgacatgt
    12721 tcaaaagcag ctgcatttgt ctgatcaaag gttggttcag ctactgtttt gatcgtctct
    12781 tcctcaattt cgatctctgc ttgcagtatt tcttctatcc gtcgtaatga ggcaaacgat
    12841 ttagggataa tgaccaatac catagctgcg atcatcaagt aagcaagtgt cagcatagag
    12901 tattcgacta cagcagtcac gacaccgatt tgtaatgctc cttctgctac aagatttccg
    12961 ccaaaccaca aaatagaagc ataaacgatt cccatgagca aaaaagcaat tggagtgacc
    13021 caagcaaata ttcggttcac tttgatcatt tgattagcat agtcagcaaa gctcttattc
    13081 gttcgctttt cttcatatga ttgattatca aaggcacgga tcatattaat cccagtaaag
    13141 aattctctca atgtgatggt gatccgatcc atttttggct ggatactcaa agataaaggt
    13201 gcccctcgtt tcatcagtcg ataaacaatc catacataaa aaacaacaga gatcaatgta
    13261 acccatgtta gtaatagaga ataacgaaac atcatgagaa tggtgaaaac gcaaataacc
    13321 ggtgctggta agatcatttg gcaaaataaa acaatcattt gctggacatt atcgacatca
    13381 ttcgtcattc ttgtcaaaag agatccagaa ctaaaacgac tgatatcctt cattgacagc
    13441 tgttggaatt tacggaaata tttctcccgc gtttggaatc caaacctagc ggaagccaat
    13501 gctgaaaagt aactagaggc aactgcagcg attgttccta gaaacgctac aagcagcatt
    13561 tcaactccaa ttttttttat aactgaggct tcgccacttg ctatacctgt gtcgatcagc
    13621 tttgctacta attttggtac acctaatgta ccggcgattt ctaaacagat catcacaaag
    13681 gtaaggagag cgagtccttt gtttttctgt aagaatgttt ttaagataga catattattc
    13741 ctcctttttt cctgatactt ttcgattcta taccttcaag taacttgaag gtcaatagaa
    13801 aaaagaataa actgaaaaag aggtgagaaa aaatgaaaaa aatgtatacg attggccaag
    13861 tatcggaact atttgatttg ccaaaatcaa cgatccgcta ttgggatgaa caaggattaa
    13921 tccgttcaag tagacaagaa gagaatgatt atcgattgtt tgatatcgat gatattttta
    13981 tgctttatga tatcgttttt tatagaaaat tagatatacc gatcaaacag atgaagaatt
    14041 tatatggaaa aacattgacg gagttatatg agacacttga tgaaacagaa agaagaatcc
    14101 ataaagaact ggtggtgatg aaacaaaaac aaaaagagat aagggagcaa aaaaaacagt
    14161 tgaaattgat gatcgataca aacgaggaag aatttcctgt agaagagatc ccttttgact
    14221 gtatgatctc aacggagttt gaagatattg tggaaatcaa aaaatttcta cctaactatt
    14281 caagctttgg gatgatgagt atgccagctt ccagttcaca gacgatgtat ggatttttta
    14341 tcgaccctag tgaagtacat ttatttactc aagacgtgat ttgggagaaa aaagatacgg
    14401 ctgtttatcg tcgattctta ttgaaatccg agatgaacca tgcggaacgt aataatattt
    14461 tagaaatcag agaaagaatg tatgaaaaag gatggaaaac aggagaagtg atcggccagt
    14521 atctattgac gaatacggat gaaaacaata tcagaactga atattatcat gcatggatag
    14581 aaatgaaaaa ataggaaata tgtgtttaag ataactcgaa aatgatggtt gttttagtta
    14641 taaagaaaaa ttttctggtt cgattacggg tttaatgtgg gggattatta gtcgtggata
    14701 gatagaattt tagtttttta gaggaaatat gtctttaaaa atggaatggt tgcaattctt
    14761 agcgatatat acgatgtaat tgtcctctta aaaaagtatg gaaaaataat tgtattgggt
    14821 gttatgtata attcatggtg aactgagact aatgagaaga aaaattcata ttgttatgag
    14881 tgagattgta tcagatgaga atgataaatt aaataggtgc ataaaagcat tacacttaaa
    14941 atacattgtt gcttacatta ttacaatatg ttaataaggc aaaaataaat agatggattg
    15001 aa
//
LOCUS       DASJTC010000069        14431 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.069, whole genome shotgun sequence.
ACCESSION   DASJTC010000069 DASJTC010000000
VERSION     DASJTC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.069"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(228..1115)
                     /locus_tag="U4B19_001973"
     CDS             complement(228..1115)
                     /locus_tag="U4B19_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2077701.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1112..1981)
                     /locus_tag="U4B19_001974"
     CDS             complement(1112..1981)
                     /locus_tag="U4B19_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2077702.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2118..3404)
                     /locus_tag="U4B19_001975"
     CDS             complement(2118..3404)
                     /locus_tag="U4B19_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2077703.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3677..4255
                     /locus_tag="U4B19_001976"
     CDS             3677..4255
                     /locus_tag="U4B19_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEN2077704.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4255..5184
                     /locus_tag="U4B19_001977"
     CDS             4255..5184
                     /locus_tag="U4B19_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2077705.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5181..6917
                     /locus_tag="U4B19_001978"
     CDS             5181..6917
                     /locus_tag="U4B19_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEN2077706.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="U4B19_001979"
     CDS             complement(6980..7816)
                     /gene="pheA"
                     /locus_tag="U4B19_001979"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEN2077707.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7813..8322)
                     /locus_tag="U4B19_001980"
     CDS             complement(7813..8322)
                     /locus_tag="U4B19_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEN2077708.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="U4B19_001981"
     CDS             complement(8315..9610)
                     /gene="aroA"
                     /locus_tag="U4B19_001981"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEN2077709.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9623..10714)
                     /locus_tag="U4B19_001982"
     CDS             complement(9623..10714)
                     /locus_tag="U4B19_001982"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEN2077710.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="U4B19_001983"
     CDS             complement(10731..11897)
                     /gene="aroC"
                     /locus_tag="U4B19_001983"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEN2077711.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="U4B19_001984"
     CDS             complement(11899..12963)
                     /gene="aroB"
                     /locus_tag="U4B19_001984"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEN2077712.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="U4B19_001985"
     CDS             complement(12996..14018)
                     /gene="aroF"
                     /locus_tag="U4B19_001985"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEN2077713.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4108 a         3143 c         2608 g         4572 t
ORIGIN      
        1 tgacaatcag gacagtcatc ggcaatatag cagcaatcac ttctttaaaa ttttttagca
       61 tgtatcctcc tcctttactc tttaagttta ccttaaaatg aaagagtttt cgagcacaaa
      121 cttaaaacgt tatcatttaa tttttctcaa cataaaataa cacacgtccc aaaagtatct
      181 ttttacttgg aaaaatgact ttgaaggaac gcgcttattg tgttttttta ttttacagag
      241 ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa atacgatggc agttggtaaa
      301 gtagcgatga ctaatccagc catgatcatt ccatagtctg gtgtatagga agcacccata
      361 gcagataaaa caagcggaac cgtccgttta tcaggagatt gcaaagctac taagggccat
      421 aaataattgt tccagctact catgaatgtg atgattgcag ccgcggcata ggtattttta
      481 gccgtcggca tatacactct aaaaaaaata cttagttctt ttagaccgtc taatcgagcg
      541 gcttctacta gatcttttgg aaaagattta acattttgtc gaaagaagaa gatcaaaaaa
      601 gcagtgctaa cagatggcag gatgacaacc aagaaactgt tgattcccaa aaccgttccg
      661 ttcaattgtg aaaacattcg atataaaggg atcatcaatg ccgcaaaagg aaccatcatc
      721 gatagcagta gaatattgaa aattaggtct tttcgtttac tttgataaat ctcaaatcca
      781 tatcctgcca ttgacgaaat cagcaatgca aacacagtgg tcaccaaagc gatcatcgct
      841 gaattccaaa gggaacgagt aaaattcagc tcattggaaa acaaattttg aaaattgatg
      901 atcaagttac tgccaatctt tatttttccc gatgtgatat ccacgggtgt attcgtcatt
      961 cccaaaacca tccagacaaa agggaaaatg gaaataaatg ataggatgct taatgctgta
     1021 tactttaata ctgacccaat tgtcgaacga agttctaatg cttgtttttt gacgttgggg
     1081 cgttcgattg tttctttttc taccatattt ttcatcgact ttctcctcct actttcgttt
     1141 ggattatcga caatatcaca ataaaggaaa caataacaaa ggaaactgct gccgcatatc
     1201 caaaattagg cgtgaattta taactcaaat tatagatgta ttgagatagg gtggtcgttg
     1261 catttgctgg tcccccgcca gtaatatttt gtacttcatc aaaaagctgc aatgtcccga
     1321 tcgtcgaagt aatggaagta aacaaaatga ttggttttaa atttgggatc gtcactttga
     1381 taaactgctg ccatttagaa gcaccgtcaa tagatgctgc ttcataaatc tccgaatcaa
     1441 tattttgcat gcctgaaagg aaaaaaatca tattgtatcc agtccaacgc caagtgatcg
     1501 aaagaatgat cagaactctc gcccaaattg gatgagtcaa ccagccaatc ggtgtgtcaa
     1561 tcataccaat gcccaacaac aagttgttta caaggccaga ttgagaaaat agacttttca
     1621 tgatcagaga ataactgacc atcgaagtga tacatggaag aaagatagcg gttcgaaaca
     1681 accctttata tttcaacttt ttatcattta aaaggttgga aataaaaagt gctagaaaca
     1741 acatgatcgg tacttgtaca agtaaataca ggaatgtatt aaaaaaagct tttttaaaag
     1801 ttgcatctgt caacatccgc tgataattac ctaacccgtt aaatgtcata ttgacagggg
     1861 gaccactttg aaaagacgtg atcaaagcca aaaccatcgg aataaaaacc atgattgata
     1921 tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt gaataaatct ttctgcttca
     1981 ttcttttccc tccaattcta tcattgatta tctacatgaa aagacaagaa agacaatatc
     2041 tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct gatgaaaaag cgaagaaatt
     2101 cactttttta ccaccgatca attagccaat tgtgcttcta cttgtttttg tgtatctgct
     2161 agtaccttgt ctgtctcttc tccattgatg attcgatgaa gagcttcagc tacgactgct
     2221 tcaattgcat aggtctcatg tccgtaattc acttctggta tttcagatgt ccatgttgaa
     2281 aaatcgtcaa agatcttttg accactgtaa aattcagaag cttcttgata agcttcttgt
     2341 tcattcgcgc taagcatcgt agatactagt ccgatttctt tcgcaagagt gcccatcagt
     2401 tcttcattcg aagcaaacgt tgcttctagg aaatctttcg ctgaatcttc tccagctact
     2461 cctttgatca cataccagcc agcgccgcca aggttagaag cttgcgcttt ttgcagattt
     2521 tctggaagtg caggtattgg tgcaatcttc cattttccag attggttttc tgctccttgg
     2581 atcgttgaag aataccaagc gccagttggg ctagaagcta ctgctcctga ttgtatcgca
     2641 gttactccag cattccagtc agaggtttgt tcgactagat cttctttcaa aagagtggca
     2701 aacaattcaa gaccatactt taaagatgca ttgtcttgga tcgtgactgt ctccccatct
     2761 tcagatgtat accattctcc tgcctgttgc ataatcatcc ttactcgacc aaggtcagaa
     2821 ggattgactt cagcgatttt ctttcccgtc ttttctttga catctcgcgc tacttgaata
     2881 tactcgtccc acttcagatt ctccatatcc tcttctgtat aacctgcttc tgccatcaga
     2941 tccgtacgat agaagtttgc tgttacgccc gaatcgaaag gaacaccgta tattttatct
     3001 cctactttgt ttaccgcaaa tttatacggt gcaaaatgat cttcctttac aatagcagac
     3061 aaattagcga atgcatctgg ataagaagtc aaatagcctt gtatcctata atcttcaatc
     3121 aagacgatat tgggcaatcc ttctgtattc ccgctcgcta aagcggtgtt cagtttctga
     3181 acgatatcat cctgagacat cgtaacaact tctatctctg tttcttcgtt ctcataaact
     3241 ttttttgctt cattcactgc tttgatattg aatgtctcat cccacgccca gacagtgact
     3301 ttttcttctt tgtctgctgt actactagca cttccgcatc ctgttaagaa acctgatgct
     3361 gccgctaaac aaacgactcc agcaattaac tttctgatgt tcatttttat ccgctccttt
     3421 tccatccatt agttgtttac gtttgctaca ttcatcatac caaaatttga aaacggtttc
     3481 tttattattc agatacttca aaaatttctc ctaaaataat aaaaaaatac aaatcaacta
     3541 tgacaaaagg aataatctta gcatctagac caaagatcga aatggtgagg tttctttaaa
     3601 ataaaaatat ccttgcattt tctcctagaa ataagtattt ttaaattaca agactattct
     3661 taaggcgggt aaaaaaatgc caatacgcgt aattcttatc gatgatgaac agacgatttt
     3721 agatggaatg aaacagttga tcaattggtc tgagcacggg tttcaattaa tagggacttt
     3781 ttccagtccg ctgaaagcgc ttgatttttc tttggtacat gcaatcgata tcgtggtcac
     3841 agatgtaatg atgcctgaat taaatggaat cgaattatcg cggctattaa aacagcaaca
     3901 acctgaaaca cagatcctta tcctctctag ctatgatgaa tttgatatgg ttaaagattc
     3961 ttttaaagaa ggcgtcgctg attatctttt aaagcctcga ttgagtcctg atttttttct
     4021 aaattcctta actgccgttt cacaaaaaat caaaaaaaag cccaacaaaa gcccaatcac
     4081 caatcgttat gagcagatct gcgagaatct ttcacagcag attgctggac aaactcgtcc
     4141 attgcttgac cctcttcttt ttgaacacac cttgtttttc tgttatatac cgaaaaacgg
     4201 ccattcagtt caaaacaagg ctctaaacaa aaacagtcga tcacccattc attgatggat
     4261 agcgacgatt cgtttactat ttatcctgtt tctacctttt cggaagaatc cggctatgta
     4321 atcaatactg attccaaaga tacacttagg aagtttgttc aaaagtttcg tcaagaagtt
     4381 tttccaggtg atttttttgt tgtttctgat tcttttgcaa tccaagatat caatgaaata
     4441 ttccaagaga tcagaaattg caccaaagga caacgtttct atcataaggg aaagaacctt
     4501 gcttatcagc atgaacttct tccgttatta accaaccacg agcagcaagc aagtagttat
     4561 ttaacaaaaa tcgttcataa agactacgct ttagctgtca aagaacttga gaatttcttc
     4621 acgacgatat tggaacagcc tattgatcca gcttatttga aacatgaggc aatcaatcgt
     4681 ttctatgcat tactcaacgc tttggctgac gagcatgttg ggaatgaaga actttctttg
     4741 ctcaagatcg cccttccttc tttattagcc gatgctgagt atttcgaaga tttttctatt
     4801 cttgtcaaag aaacacttga gaaactcacc cacttttctg caaatgcaaa aaaagacaat
     4861 ttcgatatct tgactttgat ctacgagttc gtccaggaaa attatgagaa agaaatcaat
     4921 ttacaacttt tatccgaaaa atatcatttt tcatatagct atctctcagc catctttact
     4981 gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag tccgtatcag taaagccaag
     5041 ctctttttga cggaaacacg gcttactttg acggaaatct gtgagaaagt cggctatacc
     5101 gaactgggtt atttctccag agtttttaaa gaagagacag gaatgacacc ttctcaatac
     5161 cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga gcatgaactc tttgccaaac
     5221 ttctgttcat tttgttcatc ggtctttttc tgcaagccat cgtcatttct ttgtttattt
     5281 atcatcgatc aagggatgcc tatatccagc tgttcaatca atcgaatgat gtggtactaa
     5341 aaaagattca atctgaattt gaaagtttga atgatacaat cgagaataca ttagccgctt
     5401 ttgattctaa tccagctgtc aaaagctatt tttccaatga tccagcccaa catatggaac
     5461 agcttcagca actaagaaca atccaaaaaa tgaatgactc tctttcaaaa atccatccaa
     5521 tgatcgatta cgatgtattg atctttggag aaaatggaag aacttttgtc ggaaacgaca
     5581 tgctcactgc cgtatctgcc gattcttttt ttcaatctgc gattgctcag agagtgaatg
     5641 aacgtgctgc tgatacacaa atgctttttg cacaccacgg attgactctt cgcgataaga
     5701 aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac attgaatcac gtttacggat
     5761 atgctgtttt atcgatctca tccaagcaac tagcgaatct ttttcaatct gtagtaaatc
     5821 ctgagatatc gaagatctcc ttcatgaacg aagaacagac gatcatcgca tcaaatgaag
     5881 aaaatacaat tgggagccga tcaatgattt tcaaaaaact aacagtaggt gaaacaacga
     5941 tttcaaatgg acagcagttg acccgtcttc ctctgtaccg ccaggattgt acattactca
     6001 gtcaagtcga tcttcattct ttatctaacc aaatgggcgt gatttttccg attattttgt
     6061 ataatatcgt gacacttgct tttgctggct cattggtatt tatgtatttg gatcgacaca
     6121 caaaatcgat ctaccgcttg attgattcgc taaaacggct agaggaagat ggcttaaaag
     6181 ctactgttcc tgtacaagga agccatgaag tccaattgct agggaatacg atcaatcaat
     6241 tgctaagtaa tgctcagcat cattatgaac agacgatcca agacgaaaag aagaaacgtt
     6301 cgcttgaaat ccaaactatg caagcacaga tacagcccca ttttatttat aatacgctga
     6361 ctagcttgaa atttttgatt tggcagaaaa aaacagatga agcagttcaa ggaatcgaca
     6421 gtttcattac tttactaaga agtacgattg ggaaaaaaga agaagtgatt ccagtcaagg
     6481 aagaaatcaa aagcgtacaa agttacatcg atcttttgag tatgcgatat ggcgaaagaa
     6541 tctcggctaa aataatgatt cctgatgaac tgcagtcatt atctatgccg aatatgatcc
     6601 ttcaaccaat tatcgaaaac gcctatcttc atgcttttca caataaaacg caaggattca
     6661 tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt cgaagtgatt gataacggcg
     6721 atggcttcga tactgaaaat accaaacaaa aaaatgatca tttttcagga atcggcatca
     6781 gccatgtaga tgagcggata cgcctaatgt atggcgatgc atatggctta acgatccaat
     6841 cagtgattgg gacaggaacg actgtaacga tttgtctacc gcttcttcct aaaaatcaat
     6901 cagaatatca aaaataaaga atagccaagc gctgattttt ttattttaag ttaaataaat
     6961 caatcagtat tcttgacttt catctacgcg tctcttctac tgtcagcact ggataacagc
     7021 ccaatatttg actttctcct ccgagcatct tgatttcttg gagcgcattc tctactaatg
     7081 tcattggacg gtccaacaac aaatcgatca cgaaaaaata ttctcccaag ctagttttca
     7141 atggtcggga ttcaatctta cttaagttga tttcccgcca gccaaaagct gccagcattt
     7201 tatgaagcat ccctggccga ttagcaggta gtgtcaagat gacactcatt tttactggag
     7261 ctgactgctg gctggtcatc tttcggtcac cgacgatcca aaaacgtgtc tggttcaact
     7321 cattatcttg gatattctca gctaagatct ccaatccaac atgctgtgct gtttctaatg
     7381 aagcaatcgc tgctgcgtct tctttcggat gttctgctac atacattgct gcagcagttg
     7441 ttgattctgt tgctactaaa ggaacattag gataatgcgt ttctaaaaac tgttgtgatt
     7501 gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt tgttgctgga ttgcctaaaa
     7561 gctgctgctt gataggaaga acgatttcac ttttaacttc gacttctgga tgtttggaaa
     7621 gaagatcgat tgtgtgatga acagctcctt ccaatgaatt ctctacagga acgacagcca
     7681 gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg gatggaagca tacgcagtca
     7741 attgttctgt aggaaatagc tgacaagccg cctgataagt gaatgaactt tctggcccta
     7801 gatagctgac gttcatatgc ttttgatcct ttctataatg gtatcgatga tcttttctgg
     7861 tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct tcataccaag aaagtcgttg
     7921 tgccaataat gcttttattt cttctcttgt ttttccaaga gctaaaggtc gaatagacgt
     7981 ttcatcttgt tggatacgat caactagaca gtccgcttct gcctctaagt aaagaacaat
     8041 cggctgtgtt tttaaaaaac gccgattttc tgcactttgg acaatccctc caccagtcgc
     8101 gataattttg tcttcatttg acagctttct taaaatatct gactccactt ttcgaaagct
     8161 tttttcaccg taatagtgaa aatattcggc aatcgacaat tggatatgtt cctcaatctt
     8221 tgtatctaaa tctacatact ctttttgtaa acattgagct aaccctttac cgattgttgt
     8281 ttttccagcg cccatgaaac caatcagcaa aatgctagcc atgagcttct cctcctgaca
     8341 caagttctgc taaatcgtca aaaaatgccg gataagagat tgaaacagct tcagctcctt
     8401 ctagttcgca aggtccatca gctatcaaag cagctacttg cagcatcata ccgattcgat
     8461 gatcgcctct gctggacact tttgcgccat gcagaggtgt cggcccatgg atgatcagac
     8521 cgtctgctgt cgcttttatc tttgctccca ttttctgtaa ttcttctgcc accgcatcaa
     8581 ttctgttggt ttcttttact tttaattctt ctgcgtcttt gatgatcgtg attccttctg
     8641 cttgagtagc cactaaagct agaatgggca attcatcaat cagacgcgga atgatttctc
     8701 caccaatcgt tgctttcttc aaatgactcg tcttgacact taaatcagcc gattggttct
     8761 gttcattgtg attcgtctgg gtaatcgctg cgcccatctc ctccaacaca tctaagatac
     8821 cagttcttgt tggattgacc ccgacatttt ttaacaacag ttggctttcc ggaacaagaa
     8881 gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc tggaacagtt atctgttgac
     8941 cagttaattt ttgcggtccg gtcactatga tcgttttatc ttccactgtg atccttccgc
     9001 caaattgacg aatcatttct tccgtatggt tacgggaacg ttctttttca acgattacgg
     9061 atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga tttgacttgc gcactggcta
     9121 caggcatatt gtatcggata ggtgataaag agcgggttcc tgtcacagtg attggtagat
     9181 actggtcatt ttcttctcct tgcaagtcag cgcccatctc gcgtaaaggt tccatcactc
     9241 gccccattgg acgtttagac aacgaagcat ccccgaacag agtagtcgta aaaggctgtc
     9301 cagccaaaat acccatcaat aaacgtatgg tcgtaccgga gtttcccatg tcaattgatt
     9361 cggttggttg gacaaatgag tggctaccgc gaccatgaat cttgatcacc tcttcctctt
     9421 cttcgatctc gacacctaat tgttggaagg catgtaaagt actcagacaa tcttctgcgc
     9481 gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc tccaaacatg atgctgcgat
     9541 gggaaattga tttatctgct ggtatcctaa cagtccctct taatccatgt atttgttgta
     9601 ataattgcat ttatccagcc tcctatttga tccagcaatg ataactggta ttagacataa
     9661 tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa actgatttgc agtattccta
     9721 aaatatcttc cctggtctct tggatcttca aattaatcaa agaaactccc gcttttccga
     9781 tcagtccagt tacttctgca ataacgccag gttcatctgg cacatcgata aataaatcaa
     9841 aaaaggcagg aatcgcgcct tgcttatgga caggcaactg gtctcgactt tcttttgccc
     9901 gattgaaaaa agaaaagatt tccgattggt tctcagtcat gatccattct gctatttgtg
     9961 tcatttcttg ttgccaatct gccaatcgtt ccaataatat ctgcttattg ctcaacaaaa
    10021 tatctgtcca catgacagga tctgaggaag cgatcctagt gatatctcga aagcctcctg
    10081 ctgccagttg tttggctcgc ggatactctt gattgaattg ttctgcctga ttgactaagc
    10141 cagctgccag aatatgtgga aaatgactta gcattcctgt tatacggtca tgttctcttg
    10201 ctgtaagagt tacatatttt gctttggttc cgcaaaatag ttcttgcagt ttttcaacat
    10261 cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc attttcaaat agtttagcat
    10321 ctgctgcctg tatacctgat ttatgagaac cagccattgg atgtccaccg acaaaccgaa
    10381 gatttttctg ctcagcggat gtcacgactt gctgcttcgt actgctgaca tccgtaatga
    10441 tgacgtgatc agacaagttg attctttcaa ggacatcaag ataagctaag gttgtacgga
    10501 cgggaacggc taataagata acgtctgctt gttctgcacc tgtttcaaaa tcacttgcaa
    10561 cgtaatcaac gatctgctgt tctactgctt gctcacgggt ttttgactga ctatcccaac
    10621 caactactat cagttctgga ttggtctgac gaatacaaag tgccatagaa gcaccaatca
    10681 gtccaagccc cacgataaaa acatgttgtg ccatcttatt tgcccccttt ttagtaatgt
    10741 ttcaagtagt cacggtactc cttcactgca gctttcaact caacaagcga atcacttggg
    10801 aattttgaca atagctctgt cgctaccaca gtggctacaa cattttccgc aacgacgcta
    10861 gctgcaggaa ctgctgtact atccgaacgt tctacacttg ctttgtaagg ttcttttgta
    10921 tcgatatcaa cactttgaag tggcttgtac aatgtaggaa tgggtttcat gaccccgcga
    10981 atgacaagtg gttcgccatt ggtcattccg ccttcgaagc cgcctagatt attggtacgt
    11041 cggaaatagc ctctttcttc ttcccatagg atctcatcca tcacttctga gcctggtaat
    11101 gaaccagaag tgaatccgac cccaaattct acgcctttaa aggcattgat actaaccaca
    11161 gcttgtgcga ttttggcatc tagcttcgtg tcccattgta cgtagcttcc taatccagct
    11221 ggaacgcctc caacgagcac ttctacaatt cctccgatcg tatctccatt tttctttgtc
    11281 tgatcgatca aatcttttat tctttgctca actgctggat caacaactcg tacttctgac
    11341 gcttcagaaa tttctttgat ttcttgaatc gagatatttc caggtatttc tgcttggatt
    11401 ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa gttcttccag aattttcttg
    11461 gcaattgcgc cgattgctac acgcatggtc gtctctcttg cagaggaccg ttcaagcaca
    11521 ttccgaagat ctcgatgatg gtatttcata ccgcctacca aatccgcgtg tcctggacgc
    11581 ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca cttctgctac agacatgaca
    11641 gttgtccaat ttttccaatc tttattttct accactaaag tgacaggtga accaattgtt
    11701 ttgccgtgac ggatgccaga cgtgatccga acacgatctt tttcgatctt catccttcct
    11761 cctctgccgt aaccagcttg tctgcgagcg agttcgtgat tgatttcttc ttctgacaga
    11821 ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt ctggtccatg ggattctccc
    11881 gcagtcatat aacgcatctt acagtcctcc taagtaatct ttgaatgtct ctgtgggaac
    11941 aggaacgatt tttgcttgcc caatcttttc tagcaagatg atattcaatt ggcttcctct
    12001 tgctttctta tcatgggtta tcgcgtggaa taattcatct ggatgactta gatcgtacgt
    12061 aatcggcaga tggaattttt gaagtatcct ttctaaagca gctgtcgatc cagttggaga
    12121 caagccgatt ttttctgcat gagaagtgat ggctaccatt ccaatagcta ctccttctcc
    12181 atgactgact accccatatc ctgctgtatt ttcaattgca tggccaattg tatgaccaaa
    12241 atttaaaata agtcgatttc cttggtcaaa gacatcttct tccactactt ttcgtttgat
    12301 ttccaacgta gcagtaatga ttggaacagc atgttccaga agatcttttt cgtctttcag
    12361 ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct gctgatttga tgatttcagc
    12421 gattccttct cgtaaacgac gtaccggcaa agtttttaga acctcaggat caatcaatac
    12481 accagctggc tggctaaatg ttccgacaag gtttttcgct ttgtttgtat tgactgctgt
    12541 cttcccgcca atactagaat ctacttgtgc aagtagcgta gtcggaattt gaacgaaatg
    12601 gatcccacgc atgaacgtcg aggccacgaa tgctgctaga tcacccacca ttcctccacc
    12661 aagtgcaagt atcccatcgc tgcgggtaaa atcattatct gctaaaaaat cgtataactc
    12721 agctgcttgt tctaaagatt tactggtctc acccgccggt acgattgaaa ggactggttc
    12781 aaaattagct tgtctcaatt ggaacatgat tttttctgca tatagtggag caacgtttgt
    12841 atcagtgatc acagctactt tttgaggtct ccaaatcgtt tgaagccatg atccagcctg
    12901 ttctagtgcg cctttttcaa tatgaatatc gtatttttgg ttatcaacat gtacagtaag
    12961 cattcgtttc acttctttct tttaaaaagc tggaattaat cgagtgtctg aatcttttcc
    13021 attgcttctt tcatgatcgc aacttctttc atcatgcgtg agtaagtctt ttcattcaac
    13081 gattggggac cgtctgacca agcatttgca ggatcaggat ggatctctac gatcatgcca
    13141 tctgctccgc ttgccacacc cgcacgagcc attggtggta caagttccca aattcctgta
    13201 ccatgacttg gatcaacaat cacagggaaa tgagttaatt ttttcatcaa tgggacagca
    13261 cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc gaatcccacg ttcgataaaa
    13321 atgacaggag atttgtcttc gactgcaatg tattctgcag cattaagcca ttcattgatc
    13381 gtaccggaaa ttccacgttt caatccaaca ggtttacctg ttttaccgac agcagatagc
    13441 agtttaaaat tttgcatatt acgtgcgcca atctgtagga tatcgctgta ctcagccacc
    13501 atatcgatat gcccttcatc catcacttca gtaatcactt tcattccaaa ttcatcagcc
    13561 gcttgacgga tgtatttcaa tccttcttct tctaaacctt ggaaagcata tggagaagta
    13621 cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc cggctttggc cattcgcgca
    13681 cattctctga tttggtctaa tccttcgata gaacaaggac cagccatcgt cacgaaactg
    13741 ccgtcaccaa tttttactcc gtctacatcc acgactgtat tgccaggatg gaattctcga
    13801 gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt ctactccttc atagcgtgta
    13861 aatgctcctt cttgtaaact tcgcgtatca ccttttagtc caatcactac tcgttctgtt
    13921 ccttgatcga ttgagacttt gaatccttct ttttcgatat gatcgacgac tttttttatt
    13981 tgttcttccg ttgcttcttt tttcataatt actatcattg tttttcccgc tttcttttgt
    14041 ttacttatct atcttggctg attttctcta ctttttcaag gtggttttac attaattcct
    14101 actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt ctttcctact tatgacccag
    14161 ataatcaaca aaaaaagatg tccctgtagg aaattgcctc tacagtgaac atcttcaacg
    14221 agccttaaaa agcgctgcaa aaatataaaa tagccactat agatgaattt ctctacgtgg
    14281 ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac aaacctggtt tgtttgtacc
    14341 cacgaagtca tgaatacaaa cctatctaaa atagctaaag taatacgtat tcagttttgt
    14401 aagttttcta gatttcataa aaaatcgctc c
//
LOCUS       DASJTC010000070        14257 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.070, whole genome shotgun sequence.
ACCESSION   DASJTC010000070 DASJTC010000000
VERSION     DASJTC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.070"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(433..1617)
                     /locus_tag="U4B19_001986"
     CDS             complement(433..1617)
                     /locus_tag="U4B19_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEN2077714.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(1642..2649)
                     /locus_tag="U4B19_001987"
     CDS             complement(1642..2649)
                     /locus_tag="U4B19_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2077715.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(2821..3180)
                     /locus_tag="U4B19_001988"
     CDS             complement(2821..3180)
                     /locus_tag="U4B19_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077716.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(3152..3523)
                     /locus_tag="U4B19_001989"
     CDS             complement(3152..3523)
                     /locus_tag="U4B19_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEN2077717.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            complement(3540..3842)
                     /locus_tag="U4B19_001990"
     CDS             complement(3540..3842)
                     /locus_tag="U4B19_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077718.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(3859..4329)
                     /locus_tag="U4B19_001991"
     CDS             complement(3859..4329)
                     /locus_tag="U4B19_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEN2077719.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(4304..4582)
                     /locus_tag="U4B19_001992"
     CDS             complement(4304..4582)
                     /locus_tag="U4B19_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEN2077720.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(4579..5628)
                     /locus_tag="U4B19_001993"
     CDS             complement(4579..5628)
                     /locus_tag="U4B19_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEN2077721.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(5585..6514)
                     /gene="tadA"
                     /locus_tag="U4B19_001994"
     CDS             complement(5585..6514)
                     /gene="tadA"
                     /locus_tag="U4B19_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEN2077722.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            6646..8400
                     /gene="ade"
                     /locus_tag="U4B19_001995"
     CDS             6646..8400
                     /gene="ade"
                     /locus_tag="U4B19_001995"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEN2077723.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(8498..9160)
                     /gene="trmB"
                     /locus_tag="U4B19_001996"
     CDS             complement(8498..9160)
                     /gene="trmB"
                     /locus_tag="U4B19_001996"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEN2077724.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(9230..10012)
                     /locus_tag="U4B19_001997"
     CDS             complement(9230..10012)
                     /locus_tag="U4B19_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEN2077725.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(10216..11427)
                     /locus_tag="U4B19_001998"
     CDS             complement(10216..11427)
                     /locus_tag="U4B19_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2077726.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(11420..12157)
                     /locus_tag="U4B19_001999"
     CDS             complement(11420..12157)
                     /locus_tag="U4B19_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077727.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            12295..12729
                     /locus_tag="U4B19_002000"
     CDS             12295..12729
                     /locus_tag="U4B19_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEN2077728.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            12722..13066
                     /locus_tag="U4B19_002001"
     CDS             12722..13066
                     /locus_tag="U4B19_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEN2077729.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            13246..14256
                     /locus_tag="U4B19_002002"
     CDS             13246..14256
                     /locus_tag="U4B19_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEN2077730.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
BASE COUNT         4533 a         2910 c         2401 g         4413 t
ORIGIN      
        1 ttttttcgca acaaaaaact tgattttcat acctgtgctt tcacgattca ggcaaagtac
       61 aggtggaaaa tcaagttttt attattcgat caatgaatgt ttcggatcgg ttttctagct
      121 aaacataaag cgaagaataa agtcgacagc ccacgaggct gatctaaaaa tgttttttct
      181 ttttacatat gtaatgtccc ttataaagat gaatgaaaat aaaatattgg gattttcctg
      241 aaaatcaata aataaaatgc cagtcttttc ccttttgacc acttttccaa gcaaaattcc
      301 ctaagaagat cctgtaaaaa agaagggaaa ttgaagttaa attaccattt tgcggtcttc
      361 tccgcaaaaa aaacacatgc aagaattccc tcttgcatgc gtttcatctt ttatcctaac
      421 gaatgctttg gcttagccgc gcagacgctc cacatcacgg gcaatcatta gttcctcatc
      481 tgttgggatc actaatactc ttacttttga ttcttttgta gagatatcta cttcttcacc
      541 acgcaattca ttcaacgctg gatcgatttc acaaccaaac caagacatac ctttgatgat
      601 ttcactacgt acatgtccgt cattctcgcc gataccagca gtaaagacaa ttgcatcaac
      661 accattcatc gttgtcacat aaccgccgat gtattttcgg atgcggtcca cgaaaatgtc
      721 ataagctaaa cgaatatgtt ctttttcata gttcttttca agatcacgca tatcactaga
      781 caatcctgtt aggcctagca gaccagattt cttgttcaaa acattgatca tttcatcaat
      841 atcaatctct aatttttcca ttaaatatgg gagaacagct ggatcgatat cacctgaacg
      901 tgtacccatt gtcaccccag ctaatggtgt gaatcccata gaagtatcca tggatttacc
      961 tccatcgact gctgtgatcg atacaccatt tcctaagtgg caagtgatga tttttagttc
     1021 ttcgatcggt ttgttcaata attttgccgc acgttccgac acataacgat gacttgtgcc
     1081 atggaagcca tattttctta ctttgaaatc ttcataataa gacaccggta agctatataa
     1141 ataattttgc tttggcattg ttgagtggaa agatgtatcg aatacagcca cactcaaaat
     1201 gtcaggtaaa atctttttaa acgctttgat ccccattaag tttgcaggat tatgcaaagg
     1261 agcaaattct gctaattcag caatgcggtc cattactttc tcatcgatca ctactgaatc
     1321 cccataagtt tctcctccgt gaacaacacg gtgccctact ccggtgattt catcataaga
     1381 gcttaaaatt tgcagttcaa tcaactgatc taacagcatt tttactgcta cttcgtggtt
     1441 ttcgatatcc atgatttgtt cgaatttttg attatcgccg tacttgatgg taaaaataga
     1501 atcttgtaaa ccgatacgtt cgacgatccc tttcgcaact acggtttctt ctggcatttg
     1561 atataattgc catttcaaac ttgaactccc agcattgatt gcaattgttt tagacatatg
     1621 actctccttc tcgttgaatg attacagagc agatgatttc cagtttttaa actcgttgaa
     1681 aaattccgtc accttctctg gttcttttaa tgaagcaagt ttcacaagca agacttcttt
     1741 tacttgttgt gcttgtggtc cttttttctg taagatcaaa atactttttt gcgattgttt
     1801 attacggaac aattcatctg gcaattgaat cattccttga agataacctt cttctttaaa
     1861 ccattttttg atttcttcac tttgatccgt ttctaaaaat ccactcggca tcaaaaacaa
     1921 accaaaacca tccggcttca catatttcat tgattgctct agcaataaat gatgtgcata
     1981 actatggcct tcttcggtac ttgttaaaaa ctcttttgct ttttcgtcat tcggataata
     2041 accgattggc aaatcgctga ttgcaaaatc gacaggatct attaataatt cctgcaaccc
     2101 gtcttgatga aagtattgaa catttgcttg tgtcaaatca cttgtagaag ctgcgacagc
     2161 caacaatgta tcatcaatat ccacaccaat tccttctgtt tggtaccccg cattcgatag
     2221 gtttaaaaga actgttaata acaagttgcc cattcctgcc gcaatatcca gaatcttaac
     2281 cggtgctttt ttatttgtgt atagttgttc aataagaaaa acaaataaaa aaccgatact
     2341 atcaggagtg agttgatggt tcgcctgcaa atgttctgtc tggctgccct tcaataaaag
     2401 taattgagac agtctgcgcc attcttctgg ctcgaaagaa agtttcttca actcttcata
     2461 aagagctgtg atccgttgcg ttgtctcttt tgtcggcact ccatccacca cacgtacttg
     2521 ataatcatct attaaatttt ctgcattttc gacataggct tctaaaaagg aagttcccaa
     2581 tgcattctgt aaaagctgaa tagcttccag gttttgatta aacccttgtt ccattttttc
     2641 tggtaacatg gtgcacctca tttctagata tattataaca attttttcac ctgtttaatt
     2701 ctatcgaaca attgacaaaa tttcaatcgt tttcactgtt ttttatttgt gaaacggcaa
     2761 acattttttt attaaaaaca gtgttataac agaaaagagg gcgttttcac gaacattcca
     2821 tcattctgta ctagtatctg tatcattttt tacttcttct gtgaaccgaa agataaacgg
     2881 tgagacttcc acctctaaga atagggactg ttgtacccac tgatatgtta cagatccagt
     2941 tgtgtagtaa aaaactcctt ccgaactttc ggcaagcgta gactgttccg ataaagccat
     3001 ctgcttcatt atttttgcta tataataatt tttgattcgt atattaagat ccattgacaa
     3061 acggtagctt tctaatgctg ccatcaataa aaacgtacat aaaagaaata aaagcaagac
     3121 agaaaataaa atagagccct tataacattt tctactgatc gttttttgtc catatggcat
     3181 atccatcttc tccattttca aagtgtacat aaaaagaaat tgtgtgttct ttttccaata
     3241 attgaagctc agtgatactt gttaacatag gttgatgtcc gccggaagtt cttcttttac
     3301 gtatcattgc ctgatatctt tcgatgatat actgtttatc ctcgatcgtg tagtgcagtt
     3361 gctgattttt cacagagaca ttgtgactgc tcttgagttc ttcttctaat tgtgctaaaa
     3421 atattagcca ctctttttga tctttacgct gcaaaaattc acttacttgg atggtttgct
     3481 gaatcaaaag attgaatgtg agcaatagac tgctcaatat catcagtcca attagacatt
     3541 caatcagcgt aaatgctgga attacttgtt ttttcaatac tgatcgcttc tttctccata
     3601 ttgacaaaca ctttttcgat tactccatta ttatcgacaa tttggatatc gtatgggatc
     3661 atttcttccc ttcgctctgg tcttttgtga tacatccgct gcgtcttgat atcttcataa
     3721 atcattcgaa taaatagcag ttcctcccca cttttctttt ctgcctgaag caaaaacagc
     3781 tggccaaagc tcactaagta gacacagaca gaagcgatca taagcgctgt aagggactcc
     3841 aacaaaacaa atcctgtttt attcaattct ttttgtatag cgaccactcc ctatctggaa
     3901 ttgatacgta atcgtccgct ctttttcttc ccaataaaat tgataagctt ttagtgaact
     3961 ttcattcccg gttctagcag caaaagtaat agaagcatgg cgtttcaagg taatctcatc
     4021 aggaatatct aatacttccc aatttatttt atctggattc ggaacatgaa aaatgatttg
     4081 attctcttca ctattccaat aaaagcctgt ttggatctga ttcacaatag cgattttttg
     4141 cgtagaatag attcgttttt caaattggcc gaaaaattga tagactgcca attcattctt
     4201 ccatgcagaa aatgcgagaa ctggaaaaga aaggatcgtt gtaagtaata aaatcagtaa
     4261 gagtgattca aacaacgtat atcctgatgc taaaacctta ccttcaaggc cgctgataga
     4321 tttctgcatg ttctcgtttg atatagcctt cattcaacaa ttcctccaca gtaggaactc
     4381 ttccattatt ttgaagcgca tagagctctt tttgtgtgtc taccaactgg atgatcgctt
     4441 cacgactttc ttggtctaca tgattcctat aacgcgacaa attagggacg aacaacaaca
     4501 ctaatacact gatgatcagt aagacgatta gcatttcgat caatgtaaaa ccgctataat
     4561 aagatatttt tctctttttc atggcaaaac ctcctctatg cttccgtaaa caggtaataa
     4621 aacagcggca taaactaata aaatcaaagc cgctacgccc aaaaaaatga ttggctgaat
     4681 aaaatgcatc cagtgatgaa gacgtttgaa aagctgtttt cttgtccatt cgctgtaaaa
     4741 aatcagttct ttacctaaac ttccctttgc ctctccctgt aaaacaattt tactgaattc
     4801 ttctgataga aaacgataat tctggaactg ctcagccaat gacatacctg cctccaatcc
     4861 ttgaatcaac ttagaggcaa gatgctgaat cagactttct tgtttcgttt cctttaagca
     4921 gactatgatt tgccttaatt cgaaaccttc atgaaaaagc tttccaatct ctagtgacaa
     4981 ataagccgat tgatacaagg aatatatcgg tccaatcaat aattgtctgc ttatccattc
     5041 actctgatca acaggagaca acttcatcag acgatacctt actagtaaag ccaatagagt
     5101 aaccgctagg atgaatagta gaacatacca atgaaagact tgtatcagct gaatacccca
     5161 atgctcttgc atgaccattc ctgaaccaat aagctgaggc aaaacgaaca accgcatact
     5221 aaacaagata cctagcaaaa aggtgagcaa tagcatcgga tatgcaagca gctttttcaa
     5281 atcattgcga aatccttcaa caagccgaaa ctgtttggca atttccaaca aagttgtcgg
     5341 cagattccca tgacgttcag ctaactcgat ctgtaccagt tgctctgctc catatcctat
     5401 ttgcgaaagt acctcactaa acgcttgccc ttcctttagc tgatgttgga tatttgccaa
     5461 aataacaggt ggaaacgaac ggatcgtgag taataaataa agggattctt gcagactaaa
     5521 accagataaa agcagttctc ccatggtttg tgcaaactgg ttttgctggc ttcttgttaa
     5581 tcctttagaa tagttctgac tgccaaatat tttcctgagc ttcttcataa ctttcctccc
     5641 atgatttttt tactgattct tcctccataa attgataact ccataaaaca gaaggatggt
     5701 gattggcatc ttccaaaagt tcttgataaa tgacaccagc taaacattca gatagctctt
     5761 cttttttctt tgtcagttca tggattctga ccaatgtacc ttcaagattg cgtgcatgta
     5821 cggttgcaaa aacccggtgc cctgttagtg ctgctcttac cgcagcatgt gcagttgatt
     5881 catctcttat ctcgccaatg attagcaagt ctggacgatg ccgtaatgcg agtctcaaaa
     5941 gtgtatcata agtctgtgct attttcggat tgatctgtag ctgcagaaaa aacggttctt
     6001 ctatctctac aggatcttcg atagtaatca cttgcaattt ttcttccaaa gccaaccgat
     6061 acattagaga agtcttgcct gatccgactg gtccgctgaa aaggtataat ccacgtctga
     6121 cggttttctg taaaatagtt gcaaaatctc gagaactaaa gcaacgatgg ggtgtttcaa
     6181 aagtaccgta taatagccga atcactaaac tctctcgttg aaggtaatct cctacagaag
     6241 acaaccgcag acgaacttcc tgtttttcta aagaataagt aaatgcacct aactgagctt
     6301 ttcttttttc tccgatatcc atctgtccca aatacttaaa tcttgagatc agttgctgtc
     6361 catctgccga agagactttc ttccagaatg ttcgtttttt tccatagcga aaatagatgt
     6421 aaaatacttc tgaagctggc aatatatata cgtcttgggc atgtttggca aaactttcac
     6481 ttatcatttg atccgataat cgttttattt ccattattct cccctccaca aaaaacatac
     6541 gtctttttga tctttttgtt tcaaataaaa tgaatttaca aaaaaatatt ggcaaaaacc
     6601 gaatttatgt tatacttata aaaatttatc ttgggaggca ctccattgga aacaacatta
     6661 aggaaatata tcgaagtagc aagtaaaaaa aagcctgctg acctagtgat caagcatgct
     6721 aagatcgtca acgtctttac gaaagaaatc atggaaaaag atgtagcaat ctgcgaagga
     6781 atgatcgtag gaatcggtga atacgaagga aaaagcgtac ataatgctaa tgggcaatat
     6841 ctagtgccag gattcatcga tggccatgtg catatcgaaa gctcactttt atcaccaagc
     6901 gaattttcaa aagtatcctt gcttcatgga gttactactg tagtgacaga tccccatgaa
     6961 ataggaaaca ttgctgggag tacaggtctt gagtttatga tagaagatgc aagacaaaca
     7021 ctcatgaata tcttttttat gctaccttct tgtgtacctg taaccccttt tgagaccaat
     7081 ggtgcaaatt taaatgcaaa aaaattagcc ccttttttac aaaaacccga agttttaggc
     7141 ttagctgaag tcatgaacta tcaagcagta gcaaataatg aaacagacat cctagaaaag
     7201 atccaattga tgcaccagca taagaaaaaa attgacgggc atgcagctgg tatcggcatg
     7261 gaagagttaa atgtttatcc tgcagcaggt attcggacgg atcatgaagc tacaactgca
     7321 aaagaagcta aagaacgttt ggatcttggt atgtatttaa tggttcgaga agggaccgtt
     7381 gcaaaagatc tggcatcgct actaccggcg atcacacctg aaaattcaag aagatgcttc
     7441 tttgtcaccg atgataaact gatcaatgat ttagtaaaag aaggatcaat cgatcatatc
     7501 gtccgtcaag ctattgcact tggaatcgat ccattacaag ccattcagat ggcaacattg
     7561 aatgcagcag aatgttttga tttgaaaaca ctaggtgcga ttgcccctgg gtatcaagct
     7621 gatttcttct taacagacga tcttaccaag ctgccaattc atgaagtttt ctataaggga
     7681 acattagttg ttcaatctgg gcaactccaa gaccatttat ttgctgatca ttcaaattca
     7741 tatacgtgtt gtctgccaaa actaaacgct caaccattaa atccacattc gttagagctg
     7801 cttctttctt cttcacaagc ccatgtgatc gaaattatcc cgaatagtct agtgactaaa
     7861 gatttggtag aaaaagtgga ccgacaaggc gaacgattca ttccttcaat cgaaaaagac
     7921 caattaaaga ttgcggtgat cgagcgtcac catcaaacgg gaaacattgg attaggcata
     7981 gtgaaaggat ttcagctgaa acaaggagcg atagcgacaa cgattgccca tgactcgcat
     8041 aatctagttg tcgttgggac aaacgacgag gatatgctgt atgctgctaa tcttttgatt
     8101 caaaaaggtg gcgggatgac cgttgtcaac aatcagcagg aaatagcttg tgtttctctt
     8161 cctataggcg gaatcatgac acaggaacct ttttcgatcg tctatgaaca gttgaatgaa
     8221 ttaataacta aagcacatca actaggagca gctaaaacct ttgatccttt tttgacactt
     8281 tctttcttaa cattatctgt catacccgaa ttgaagatca ccgacaaagg attattttca
     8341 ttttctcgct tccaattgat tccaccttgt ggaaaaaccg atgaaataaa tacattttaa
     8401 atcaaataaa tcccagaaaa tacattacat ctgtaatgtt cattttctgg gatttattta
     8461 tggatattaa tgtttttatg aaagcagaaa gttagtttca cagacttttc tttttggtct
     8521 tatcttgaaa ttgagcttct actcgataaa tacgttgacc ttttgaagaa aatttttctt
     8581 catactccgt cataatattg ccttcaaatt gactttgatg taaatctaac catacctgtt
     8641 tgaggatcat gccatattga gaaaaacttg ccagagaata ctcaaacaat ccttgattat
     8701 ctgttttgaa atggatttct ccatttggac gaagaatctg ttcatctaca gcaagaaaag
     8761 tcttatatgt caatcgccgt ttttcatgac gtttttttgg ccatggatcg gaaaaattca
     8821 gatagatttg atctacttcg ctgtcagcga aatactctgt caacgctgat ccatccacat
     8881 gtagaagttt caggttagga agcggttgtt cgatcaactt gtctagtgca atcgaaacaa
     8941 cactgacctg catttctact ccaatataat tgatttcagg atgggctttt gccattccag
     9001 tgatgaactg gcctttcccc ataccaatct caatgtggat tggatgatcg ttttcaaaca
     9061 attcattcca ttttcccttc cacaatgttg gatcactaat tacaaaattc gggtgagccg
     9121 ctaacatttc cgctgctccc ggacgatttc ggacacgcat tatttatctc cattcctttc
     9181 ctctaattaa aaaggtctgg ccaaatctcc gcagagtttg ggccaaacct cagccactga
     9241 aaattcgttt taattgtaaa atttcttcgt tcatccgtcg atgatcttca cgtttgcaat
     9301 atctggtgat ttcttggagc aaattcatgg ccgcatacca atgaaatttt tcaagcattt
     9361 cgtcagtcgg gcgtatgcca taactgatca gccactgatt ccagctagac agtggcacat
     9421 aatttcctaa aatcgttgca atatccaccg cagggtctgc aaacatgatt gaatcccaat
     9481 cgactagata taaataattt ttacatacta accaattacg atggtttaca tctccgtgta
     9541 ctactgataa gttttcctta tctattgacg ggatctgctc acgaagaaaa cgatggacaa
     9601 tttgcagaaa ttgatttttc tggagttctt ccggtaattc tttttcatat ttttgcagca
     9661 tcttctccgg cgtcatgaca cgaccaccga tacgggtcag catggtttta agagaatttg
     9721 aatgatgtaa atggtacaaa acatcaatca cgtcgtttct ttgaccaatc tcattaggag
     9781 tcagcaagcg accttccagc cactcttgtg ctgtcaaagt atcgccattc cccgttcgtt
     9841 ttgtccaaac aagttttggt gtaattcctt cccttgaaag agcagccaac atcggagtcg
     9901 tgtttcgttt aataaagact ttgtcctcat cacgcatgcc tatgtatgtt tttcctgtgt
     9961 cgcctttgat gggacgtaac cgccactctt ggtctaactg aaactccatc atgcattctc
    10021 ccgtcctctt actctttgtt ttgcagtact tattattcta gtcgcctgaa cactacacgt
    10081 caagcacata tacgttcttt ttcatttaat tgccattcaa aaaaccagct tttctcaaaa
    10141 aaaatagaac gaacagcatt cttactgtca acgctctatt tttcattctc atttttaccc
    10201 tacacagctt tgttttcagt ctgacatctt cttcaaacga gttggtacat atagtttaac
    10261 aaagagcata gtaacaatca aataagccgc tagcggaacc catcgctgct gcccatctaa
    10321 tacgactgca gcaatcaaac caaaaataac cgacgcgata attaataccc atgtgagtag
    10381 atactgaatc gcctgcttct tctgattctt atggattgga taaagctgaa cgagaaccat
    10441 atagcggaac tggttcgcta aaggtaacag ttggaaaccg atcaaatata aaaataagaa
    10501 accgataatc agactaaaat aaaagtcgac tactccggca atcaataacc cacctataat
    10561 tactaatcgg aaataaagac cactaaaatc agaacctctt gccaatcttc ttgcaaacaa
    10621 gtaaagataa gtgttttttg attctctttt aatatgagtc aatataggat ccaaatagcg
    10681 tcttcttttg atctttgccg taatttcagg aacatctgtg aataaattga taaattgata
    10741 gatctgatgc aacctatgtt cttctacatg caccattttc tcccaatcca atcctttttc
    10801 ttgttgcttc cacaaaaacc agtaatatag acttgcctgt actaatgcac ctgctattcc
    10861 tgcccatatt agccaataaa atgatagaaa caaaaagatc agagaagaaa aaaaccagcc
    10921 taatttccaa taacgaataa cttgttgatc tagtcgaaac aattctgctc tcttgatttg
    10981 cagatgactg aatttcaaac tccataatat gatcagatag acgaaaaaca tcgaaaagga
    11041 ttgctccgta ctaaccacca ctaatggcat ggccaatccg catgccaata tcaaaaagat
    11101 caacggaaaa atgcaagaat agcgaaaagc tcgttccaaa taggtttgca tttctttttc
    11161 tttaggaagt aaaaaaacaa tatctgctgc ctctgcaaaa gtagccatct tacccacgaa
    11221 taaacagagc atccaaaata cagagattat gagccctcca attgcaaacg gtggctgcag
    11281 ggttttcaac caattagaat aatataatcc cacaccgcca atcaagaaaa gacaaactaa
    11341 aacaaaatga tcgttcaata cataacgcat atatttcatc attttctttt gatgtcttgc
    11401 taaacgtttt tgaaaaaaac tactcatgtg cctgctcctt tgttaaagca atatagattt
    11461 cgtccaaaga agaatccggc agctgaaatt cttttctcaa catctcaaga gaaccgtctg
    11521 ctcgtatctc tccgttatgt aagaccacaa aacgatcaca atatttttct gcagtagcta
    11581 aaatatgcgt agacatcaaa ataccggctc cttctttttt catttctacc atcaattcaa
    11641 gcaatgcatt gatagctagt ggatccaagc ctaaaaatgg ttcgtcgatg atatataaag
    11701 aaggctcgat caaaaaggcg cataagatca tcactttctg tttcattccc tttgaaaaat
    11761 tagctgggaa ccagtcaaga cgattagata accgaaaagt ctccaacaac ttttctgctc
    11821 ttttcattgc tatatccata ggtatatcgt aggccatcgc agtgacttcg atatgttctc
    11881 tcaatgtcaa ttcttcatat aaagaaggag tctctggaat atacccaatt tgtttgcgat
    11941 attcttccgt atttttcttt aaggatagat gattgatttc tatcgttcct tttgaagctt
    12001 ccagcagacc gataatgtgc ttgatggtcg tacttttccc tgctccgttc agtccaatca
    12061 accctactat ctctccaggc tgtacatcga aactgatatc ttttaacaca gggagatgtc
    12121 cgtacccccc tgtgacatga tcgataatta aactcattct ttttcctccg ccatttattt
    12181 ttatttatta taccacgaag aaaatgacgg tattttgttt ttttccttac aatagcttta
    12241 acttatggta aagttaagct gaagtattta ttctttatag aaaggatgtt ttatatggaa
    12301 aactgtattt tttgcaaaat catcaatcaa gaaatcccaa gctataaaat atatgaagac
    12361 gacaaggtct atgctttttt agacatctct caagcaacga aaggacacac attagttgtg
    12421 cctaaacgtc atgtagcaga tatttttgaa tatgatcccg aacttgcagg agaggttttt
    12481 tcgcgtgtgc ctaagattgc tcaagcattg gaaaaagctt ttccagaaat gaaaggtctt
    12541 aatatcttga ataacaaccg tgaactagcc tatcaatctg tttttcattc acatatccat
    12601 ttagtccctc gctacagcaa agaggatgat ttttctattc actttgtcaa tcatcaagat
    12661 agttatggat cggaagaact aaaagctatt caagaaacaa tcgtaaagca ggtgtcctgc
    12721 gatgattaaa aactttttca aaggactgtt atttggagct gctgctggca ctgtcggagg
    12781 tctgttattg gcgcccagga gcgggaatga aacaagaaag aaactgatcg aagaattgga
    12841 agaagcaaca actctgacaa atgatttaaa taacagcctg aatcatttta aaagcgcatt
    12901 gatcgaaaca aaagaaacag ctgaatctgt gattcctgct ttccaagaag caatcaaaaa
    12961 agatatcgaa gattttaaat tccaagccga accacgtatc acacaaatca atgaacaagt
    13021 agaaaaacta aaagctgatc tgccagagta tcctgaacaa atataataat gattattttc
    13081 tcaacagatt taactatata agtgagaaaa gtttgcgtat ttcctaggaa tgtgatgaaa
    13141 ttttgaattt tgtttgaaac taccacgaca acgtttttaa aggaaatggg atatggtata
    13201 gttaatatat aaaaataata actaattaca ggagtgcaaa ttttaatgaa aaaaaaatca
    13261 attattttgg cagctactag tgcgttggct gttttaactc tagcagcttg ttcaggcgat
    13321 actaataaag atatcgcaac gatgaaaggc ggaacgatca ctgtatctga tttctatgat
    13381 gaagcaaaac ttgaatcatc gaaccaatcc ttagtacaac ggatgatcat ttataaagta
    13441 ttcaataaca aatatggcga taaagtaacc gataagcaag tggatgcaga atacgacaaa
    13501 caagcgaaat cattaggtga tacttttgaa tcacaattag aagctgctgg atatacgaaa
    13561 gatacttata aagaatatat tcgtaacaat ttagcttttg aagctggctt gaaagctcac
    13621 gtagatatta cagatgacga tttgaaaact gcttggaaat ctttccatcc agaagtagaa
    13681 gcacaaatca tcaaattatc aagtgaagat gaagcaaaag atgtgaaaaa atcagcggat
    13741 gatggagatg atttctctaa attagccaaa gacaaatcaa ctgatacaga gacaaaagaa
    13801 gatggcggta aagttaaatt cgactcaacg acaacaacta ttcctgcaga agtaaaagaa
    13861 gcagctttca aattgaaaga cggtgagatc tccgatgtta ttactacaac aaatcctact
    13921 tcttatgcga cagaatacta cgttgtcaaa atggtgaaaa accaaaataa aggcaatgac
    13981 atggataaat acaaagatca actaaaagat atcgctactg aaactaaact aagtgacaat
    14041 gcattcacaa ctaaagtaat cggtgaagaa ttaaaagatg caaatgtaaa aatcaaagat
    14101 gatgcctttg aaaatgtgtt gagtgcattc acaacaactt catcaagtac gaaagactca
    14161 agtgaaacta cagcttcaac aaagagttct gacacaaaat caactgattc aacaaaagaa
    14221 tcttcaactg aagaaacaac tgattcatca aaataaa
//
LOCUS       DASJTC010000071        14139 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.071, whole genome shotgun sequence.
ACCESSION   DASJTC010000071 DASJTC010000000
VERSION     DASJTC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.071"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(51..3725)
                     /gene="rpoC"
                     /locus_tag="U4B19_002003"
     CDS             complement(51..3725)
                     /gene="rpoC"
                     /locus_tag="U4B19_002003"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEN2077731.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="U4B19_002004"
     CDS             complement(3783..7400)
                     /gene="rpoB"
                     /locus_tag="U4B19_002004"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN2077732.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8128..9096
                     /locus_tag="U4B19_002005"
     CDS             8128..9096
                     /locus_tag="U4B19_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2077733.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9114..10019
                     /locus_tag="U4B19_002006"
     CDS             9114..10019
                     /locus_tag="U4B19_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2077734.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10016..10822
                     /locus_tag="U4B19_002007"
     CDS             10016..10822
                     /locus_tag="U4B19_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2077735.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10958..11947
                     /locus_tag="U4B19_002008"
     CDS             10958..11947
                     /locus_tag="U4B19_002008"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEN2077736.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12264..12788)
                     /locus_tag="U4B19_002009"
     CDS             complement(12264..12788)
                     /locus_tag="U4B19_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEN2077737.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13075..>14139
                     /gene="scm"
                     /locus_tag="U4B19_002010"
     CDS             13075..>14139
                     /gene="scm"
                     /locus_tag="U4B19_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN2077738.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT         4003 a         3047 c         2793 g         4296 t
ORIGIN      
        1 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
       61 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
      121 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
      181 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
      241 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
      301 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
      361 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
      421 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
      481 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
      541 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
      601 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
      661 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
      721 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
      781 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
      841 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
      901 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
      961 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
     1021 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
     1081 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
     1141 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
     1201 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
     1261 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
     1321 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
     1381 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
     1441 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
     1501 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
     1561 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
     1621 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
     1681 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
     1741 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
     1801 tctgttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
     1861 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
     1921 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
     1981 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
     2041 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
     2101 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
     2161 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
     2221 atatcttggg aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
     2281 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
     2341 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
     2401 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
     2461 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
     2521 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
     2581 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
     2641 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
     2701 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
     2761 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
     2821 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
     2881 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
     2941 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
     3001 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
     3061 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
     3121 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
     3181 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
     3241 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
     3301 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
     3361 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
     3421 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
     3481 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
     3541 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
     3601 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
     3661 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
     3721 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
     3781 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
     3841 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
     3901 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
     3961 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
     4021 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
     4081 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
     4141 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
     4201 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
     4261 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
     4321 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
     4381 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
     4441 actggcgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
     4501 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
     4561 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
     4621 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
     4681 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
     4741 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
     4801 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
     4861 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
     4921 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
     4981 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
     5041 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
     5101 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
     5161 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
     5221 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
     5281 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
     5341 tcaacgacac cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
     5401 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
     5461 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
     5521 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
     5581 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
     5641 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
     5701 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
     5761 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
     5821 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
     5881 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt
     5941 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
     6001 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
     6061 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
     6121 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
     6181 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
     6241 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
     6301 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
     6361 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
     6421 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
     6481 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
     6541 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
     6601 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
     6661 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
     6721 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
     6781 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
     6841 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
     6901 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
     6961 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
     7021 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
     7081 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
     7141 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
     7201 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
     7261 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
     7321 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
     7381 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
     7441 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
     7501 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
     7561 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
     7621 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
     7681 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
     7741 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
     7801 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
     7861 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
     7921 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
     7981 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
     8041 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
     8101 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
     8161 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
     8221 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
     8281 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
     8341 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
     8401 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
     8461 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
     8521 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
     8581 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
     8641 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
     8701 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
     8761 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
     8821 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
     8881 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
     8941 ggtaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
     9001 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
     9061 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
     9121 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
     9181 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
     9241 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
     9301 cttgtcttct aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca
     9361 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
     9421 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
     9481 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
     9541 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
     9601 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
     9661 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
     9721 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
     9781 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
     9841 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
     9901 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
     9961 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    10021 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    10081 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    10141 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    10201 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    10261 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    10321 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    10381 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    10441 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    10501 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    10561 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    10621 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    10681 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    10741 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    10801 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    10861 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    10921 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    10981 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    11041 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    11101 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    11161 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    11221 acaaacgatg ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg
    11281 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    11341 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    11401 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    11461 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    11521 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    11581 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    11641 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    11701 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    11761 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    11821 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    11881 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    11941 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    12001 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    12061 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    12121 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    12181 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    12241 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    12301 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    12361 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    12421 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    12481 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    12541 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    12601 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    12661 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    12721 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    12781 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    12841 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    12901 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    12961 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    13021 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    13081 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    13141 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    13201 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    13261 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    13321 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    13381 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    13441 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    13501 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    13561 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    13621 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    13681 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    13741 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    13801 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    13861 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    13921 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    13981 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    14041 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    14101 acagatacat ctaccacaag tgatagctca gattcatca
//
LOCUS       DASJTC010000072        13792 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.072, whole genome shotgun sequence.
ACCESSION   DASJTC010000072 DASJTC010000000
VERSION     DASJTC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.072"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            44..238
                     /locus_tag="U4B19_002011"
     CDS             44..238
                     /locus_tag="U4B19_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077739.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            507..806
                     /locus_tag="U4B19_002012"
     CDS             507..806
                     /locus_tag="U4B19_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077740.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(903..1337)
                     /locus_tag="U4B19_002013"
     CDS             complement(903..1337)
                     /locus_tag="U4B19_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2077741.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1569..2279)
                     /locus_tag="U4B19_002014"
     CDS             complement(1569..2279)
                     /locus_tag="U4B19_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEN2077742.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2269..3129)
                     /locus_tag="U4B19_002015"
     CDS             complement(2269..3129)
                     /locus_tag="U4B19_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEN2077743.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3134..3772)
                     /locus_tag="U4B19_002016"
     CDS             complement(3134..3772)
                     /locus_tag="U4B19_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEN2077744.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3787..4086)
                     /locus_tag="U4B19_002017"
     CDS             complement(3787..4086)
                     /locus_tag="U4B19_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2077745.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4129..5613)
                     /locus_tag="U4B19_002018"
     CDS             complement(4129..5613)
                     /locus_tag="U4B19_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEN2077746.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5633..6094)
                     /locus_tag="U4B19_002019"
     CDS             complement(5633..6094)
                     /locus_tag="U4B19_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2077747.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6112..7179)
                     /gene="ulaG"
                     /locus_tag="U4B19_002020"
     CDS             complement(6112..7179)
                     /gene="ulaG"
                     /locus_tag="U4B19_002020"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEN2077748.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7397..8167
                     /locus_tag="U4B19_002021"
     CDS             7397..8167
                     /locus_tag="U4B19_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEN2077749.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8440..9267)
                     /locus_tag="U4B19_002022"
     CDS             complement(8440..9267)
                     /locus_tag="U4B19_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2077750.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9835..11070
                     /locus_tag="U4B19_002023"
     CDS             9835..11070
                     /locus_tag="U4B19_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEN2077751.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12069..12329)
                     /locus_tag="U4B19_002024"
     CDS             complement(12069..12329)
                     /locus_tag="U4B19_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEN2077752.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12356..13657)
                     /locus_tag="U4B19_002025"
     CDS             complement(12356..13657)
                     /locus_tag="U4B19_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2077753.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
BASE COUNT         4436 a         2824 c         2172 g         4360 t
ORIGIN      
        1 tatttgaata ttaactaaaa acagcaagaa agaagaggat caaatgtatt atgtagaagt
       61 aaaaacaaaa ggcgtcaaaa acaaacaata tgtgaaaggt atcagtaacg aatatccttt
      121 acttggttca tggaaagaag ctgctccttt ttccaaacca tgtgccataa aaataaagaa
      181 cgagctagaa aaagaactga cttgtggtaa agctgttgta gatatcatcg aaaagtaaat
      241 ccaatgtata gaaaggaaga tcacacatga aaagcgacgt acaaattgct aagattatag
      301 aattagaaga agaagacgca gtgttgacaa ttgaaaaaaa ataaatgttt gtataccata
      361 tgattatttc aatatgtatc ctggaattgg agatctcgta gagctatatc aagatgaatc
      421 aaatatactt gtcattcatc atgaaatata gagacttata aaattgttta tttttataat
      481 gtatcagtat agaaaggaaa taaaacatgg attatactgt tgaatttgac agtgaactac
      541 ttaagcaatt tccacaagaa gaacatttca tgtataaaga agcaatttcc cacaaagtga
      601 cagagtattt ggagcataat ttttttcgta taaagccagt gcgtactcgt tcggcaaaag
      661 gaatatacga gatgaaagtc aaaattaata aaaaaaatta tcgcatagca tttgctttaa
      721 aaaaaaatca tgtgttcatt ttttatatta gttcgaattt gcaaaaaatt gtatttgata
      781 aagaggtgag taaaaaaata aaatagctga gcattggagg atagattgag tgatactaag
      841 tctatcctct tgtttttcta aagaactaag ctagaatttt ttcttttgta cgttattata
      901 tgttatttgt cattaccaaa aagcaccttt tttcttctag tatctatgac ataagaaata
      961 ccacccattt cctttacttg aacaaatcct actgattcat aaaatttttt tgttttctca
     1021 gtatcatctg tcaataattc gatttggact ggtccataac tagtgatctt ctccaaagct
     1081 tcattcaaaa gcactttgcc tattccttgg cgctgatacc tcggatgaat gaggatatct
     1141 tgaataaatg cgatcgtcgc aaaatcagtg atccaacgaa cgattccgat aatcttccct
     1201 tccccattac gctttatcag agattcagat tgttcaaatg ctctagctaa ccttgcagtg
     1261 tcttttgtgt aattcgtcca accaacgctc ttatataatt cagaaatctc atcgatcata
     1321 agggaatcaa tattcatttt tttcttcctt tcttatacaa ttctccctct aataatacag
     1381 ttctttgact atttaatcta tcacatataa ctctcctgta aagattctct cgattcctta
     1441 aaattcctct ttccttttag aatgcatact ttttgataaa taaaaaggtt gtgataaatt
     1501 ttcttcacaa cctctgtttc tctctatcaa gtgttatctt ttaattttct acccttaccc
     1561 tagcatctct attgcccgta gtatgcatac tttccatgtt tgcgaaaata atgtttatct
     1621 agtaaatatt gcgggatttc acatatattg gggttgatca gctcgttttc tttggccatc
     1681 agacaaattt catctaagat caaactgttt tctactgctt tcataggtga atccccccat
     1741 gtgaacggtc catgtccata gacaagtact ccagggactt cgtttggatc taattttctt
     1801 tccttaaacg tctcgacaat cacattccct gtatgaactt cgtaagcttc tcttacttct
     1861 tctttcgtta attggcgtgt acaaggaacc ttaccataaa aagcatcagc atgagtcgtg
     1921 ccataagctg gcaaatcgcg tcctgcttgc gcccacatga cggagtgcgt ggaatgagta
     1981 tgagtaatag cagttatatc ttcaaaagtt tgatacaaaa caacatgtgt ggggagatct
     2041 gaagatggtt ttaagctatc ttcttctatt atatttcccg acaaatctgt taccaccatt
     2101 tgatctgctt gcatgcattc atatctgaca ccgcttggct taatgacgat gactcctagc
     2161 tcacggtcca cttcactcac attgccccaa gtcaatttga ctaatccgta tcgcggcaat
     2221 gccagatttg cttcaaggac acgttgcttc attgctgcaa ttttttcttc acaagacatc
     2281 atagccagcc tccttcaatt tagaacttaa gtagtctttt gcctgctgga tttctcttcg
     2341 aaagtcttgg cttttttcac tccacatttc aatcagaaat gttccatcat aatctagacg
     2401 tttcaaatgt cttaataatc caagaaaatc aacacaaccg ctaccaaaag gaacatcacg
     2461 aaactggcct gaatattctt ttgttacagg gtaagtatct tttaagtgga tagcagtgat
     2521 ataatcgatc cctttctcaa gttctcgagc cggattattt tccggccatg cggacaaatt
     2581 gcctaggtct ggatatactt gtagaaaagg tgaatgaatc tgttctttga tttctaaaaa
     2641 ttttgaaatg gagttcataa atggatcatc catgatttcc acggaaagaa tgatgccata
     2701 tttagaagct tcctttacgc tttcttttat tccttcgata aagtcttctc tggaattcat
     2761 tgatttctct tcataataaa catcgtatcc agctatttgg attactttga cgcttagatt
     2821 atgcgccaaa tggattgctt ttttcattag ttgtttcgct gcttttttct tttctggatc
     2881 agcagaacca aacggaaatc gccgatgcgc acttaaacaa atcgaatgga tacgtacacc
     2941 ggagctgaac atctcttctc tcagctcagc tatcttttct tcagaccaat ctaatcgtgc
     3001 taaccgctca tccgtctcat caatcgacat ctcgataaaa tcgaagtcca tttcttttac
     3061 aagggaaaaa cgttctttcc atgaaatatc tttgggtaac gctttttcat aaatcccaat
     3121 ttgagccatt tccttacccc cagatacgaa tgatctcatc tttgaagttt tgtgcggcct
     3181 tcatcggatc atttgctgct gtaataccac gaccagtgat aaaggtatac acatcgatcc
     3241 cttcaaataa gtgtaatgtg tgtggattta atccaccagt tacagaaaca ttaaagccta
     3301 gttcaatcag ttttttgatc ttcgataaat ctttttcacc ccaagtttca ccagataaca
     3361 gcgcatcacg gctttgatgg tagattacct gccggatacc gatgttatgc caatccatgg
     3421 cttgttcaaa agtccaattg ccgtagagtt ctacttgcaa ttcacctatt tctttttgtg
     3481 ctgcctccat agttggaatc gttgcacaac aaatgactgt catccagtct gctcctgctt
     3541 ttgcaacatt actcgccact gttcctccag cgtctgcaca tttcgtatca gcgactagtt
     3601 ttttatctgg gaacatactg cgaatacagc gaatcgcttt ttgtccttcc tgtaagcaaa
     3661 gaattgtgcc cacttcaata atatctacta catcaccaac tttcataaca tctcctaatg
     3721 catgttctaa gtcattgtga tctaacgcaa tttgcaaatt cggcctgctc atcttagttc
     3781 cttcctttac ttgttttttt ggtaccactt tgtttcagca aatttttcac tgatttcttt
     3841 gactgacatc acatttttga taccgataac tgtaactcct tttttctctg cctgagtaaa
     3901 catattcaaa aaattgactg gcgtaaaaac tacatcgtat tggccagctg aactttttcc
     3961 ctctgaaatg gcacaatgat ggattttggt aattggtacg tttagttctt tcattgcttt
     4021 ttccacactt ctcatcatca tcaagcttgt tcctgatcca tttgcacacg atactaaaat
     4081 tctcatctta atccctccat tatgagtgtt gtctaatcag attttctttt atactctctg
     4141 cttttctgca tatgtgtcat agtcttctac aatcaaaaaa taaccatcag gatttttccg
     4201 atattgtaac tgcggaatag ccaataaacc aatgacaact acggcaattc cagcataacc
     4261 taggactttc atgattactg taaacaatgg ccaagctgtt gcccagtcga acatcccgat
     4321 ataaccaccg tatgttgaca tcccaatcca tgtagcgaat aatgctgaac cgcctacttg
     4381 tatgattcct gaaagaaacg ggaacaggca agcggctttg aatccacctc ggttattggc
     4441 ataaactgct ataacggcat tatcaaagaa caacggaatg aaaccggcta tgacgatcgt
     4501 tggtgatttc aataaaatca gtaaaccaat catcaaaaac tgtcccaaag caccaaacaa
     4561 gaacccaatc gttaccgcat ttggcgaacc aaagccaaaa gtagcagcta cgtcaattcc
     4621 tggaacagct cctggtaaaa tcgtgtttga gattccttgg aaagattgag ttagttcatc
     4681 gacgaatgtc cgaacaccta attgcaagat cgccagatat accgcaaaat ttaaagaagt
     4741 cgtcataatg tagaataaga aactctgccc ttcttgcatg aattgcgctt gaatcaaata
     4801 atctttgcct agtacaatca gaatgatccc aaagaaaaat agcatgagta acgaagtagc
     4861 aaccatattc tcattgaaaa tcgacaagaa tcctggtaat ttgatatctt caattttttt
     4921 gtcttgcttg attgatctat ttttcttttc atcccgcttt ttcatccact cagctaattt
     4981 tgcaaatata aagattccga acatttgctg atgtgccact gcaaatccgg caccttctgt
     5041 caattcctgc gtgattccta ctgttaagtt agaagctact gtccaataac aacctaaaat
     5101 cagtcccata aaaatcaatg cttgtatcct acctagatca ggaaagcaaa acagtaaaat
     5161 ccaaaaagcg gtagctgctt gttgaacctg aacgtttccg gtagtgaaaa cggaacgcag
     5221 tttcgtatat ttctgaaaac ggactaataa gatatttaaa acaaatgcaa tcagtaataa
     5281 aatcatcaca tctccaaagg tacggccaaa tgtctccatt aatccagcat ccacagcatt
     5341 ttgtccaaaa taaggatctg taaccatggc atccaaattg aaccgttcct ttagtccaac
     5401 caagattggc cgaaaattat tgaccaaacc accagagcca acagtcaaaa taaagtaacc
     5461 gaccgttgcc tttaagaatc cagcgatgca ttcatgaagc gggcgtttca gcaaaatata
     5521 gcctaataac acgataaaac caatcaggta tgctggctgc gtcaagatat ttgttgcaaa
     5581 atacgtccaa atgcttaaca aaaattcccc catctttttt ctcctctatc aattaattat
     5641 attctgccat cacaacttgg taatcatcga tggagttcgt agagatgaga cgctcaacca
     5701 atccatctgt cattaataat tcggataatt tttggatatt ttctagatgt tcttctggat
     5761 cttttgctgc gagtgtaaaa aacaaactag ccgttttctc ctcattctcc ccttcacttt
     5821 caaaacggat tggctgtttc aacttggtaa atgaaatagc tgttccaaaa acaccatcac
     5881 ttttttctga agaatgcggc attgctactt gaggtgcgat cacaatataa ggaccgtatt
     5941 tttctacaca atggatgatc tcctccacat acgtttcatc aataatttcc ttatcgatca
     6001 atacaagaca gctttcggcg atagcttcac gccaatccgt gatttcttta tctgggtaat
     6061 gaatcaattg atggtcaaaa aagtatttca gcaagaaact ccccttcttt cttataaaaa
     6121 cgacggaaac ggtgtatcat tttcaatcga gaagacatct tcaaatcctc gataaaaatt
     6181 aaactcaaga tcatctttgt ttgtcggata gatgaatttc ccgcctactt gccagatata
     6241 aggtttaaat ccatacttca ggcgatcctt tttgaatttc caaatttcag tgatctcttt
     6301 tggatcagcc ataaaattag accaaatatc gtagtggaca ggaatcacta cttttgcatt
     6361 caatgactcc gccatgcgca gcatatcaac agaagtaact ttgtctgtta tccctcgtgg
     6421 gttttcacca taggccccta aacagacatc gattttgtgt tcattaccat gtttggcaaa
     6481 taaattggag taatgtgaat ctcctgcatg ataaagattc cctcctgatg tttcgaataa
     6541 atagtttact gcaatttgat ccatctcctg aggcattttt ccttttaata cctcttctgg
     6601 atcttcacaa gtgaccaacg ctgtccgatc aaacgcttct aaagctacta cattgatgtc
     6661 tttgattttg actgcatcac ctggctttac tgtaatcgtt ttttcttcag gaattcccca
     6721 tttttgccac gtgcgaacca cttcttttgg cccaatgaat gttgcttcag gacaattttg
     6781 atgaactgct gcggctgtat ggatatctag atgatcggaa tggatatgtg taacgattaa
     6841 agcgtctaca ttttttatcg caaaaggatc gattacgaaa ggctgagtac gtaaattagg
     6901 ctgcatattt tgaacgccac tcatacgcat catctgatgg ccttttttca tcatgccatt
     6961 cccatgacta cgttttcccg tcccacacca tagatcacat aaaatattcg ttccttcatg
     7021 acttttcagc caaattcccg tacagcccag ccaccacata gcaaaagttc caggatccac
     7081 tacttctgtt tcgatttctt cattcaagta tgtgccccac tcaggaaatg tttctactaa
     7141 ccacttttct tttgttactt cattgacagt tttactcatt tgcttgctcc ccccatatcg
     7201 ttgttccaac ttacaagtaa atattaatgt gtatcagcat ccatgaaaac ccttttatat
     7261 gaccgtttgt tttttatatg attttctttc agtaatcaat gattgtttca gtataataaa
     7321 gacagatatg aatgtttaaa tgaacaacta gtcaaataaa tccatggatc aagatgtccc
     7381 tcaggaggca aaagtcatga aaaactcact tgtcaatatt gaaaaacgtc aaaaagaaat
     7441 tttggaactg cttcagcaga ctgaacaact atcaactttc gaattagctg aatcccttga
     7501 tgtatctatt agtacgatac gaagagattt aaatttatta gaagaaaaaa acgagatcat
     7561 acgcaattac ggttattgtt cttataatta caaaaaccag actgattttg atcaatctgg
     7621 tcctgaaagg attaaacaag cgattgccag agaagctagt aaatacatta acgattatga
     7681 tacgctgttt atcaattcta gctcgactgc gttgaaaaca ctggattttc ttcaatctaa
     7741 ccatttgaca gtcattacaa acaacgtgaa aatcaattac tctccgcaca aagataattg
     7801 tagttatatt ttaactggtg gagagatgcg ctttccaaaa gaagcactag tgggcgacat
     7861 cgcattaaat acgatttctt cagtcaatgc agacatttgt atcattggct gtagcggagt
     7921 cgatgtagta catggggtca caacaaagat attaaatgaa tcaacgatca acgaaatgat
     7981 ggtcaacaag acaatcaaat gtaagatttt agtcgctgat catcgaaaaa tcggactgac
     8041 atcaaagttc aagattgctg atatttcagt ctttgattat ttgatcactg ataaattttg
     8101 ttctgaaaaa gtattagagg agatcagaaa tacggggata aaaaccattc aagtagattc
     8161 attttaagaa ccaggacgta aatatgtaaa taggattatc aaaaaaatga ataacaaatt
     8221 tatcgataaa tgaaaaacac gctacgctca aattgataaa ggaatcccaa gatgtatcca
     8281 tccaatcgat accatcataa tctattgctt cttttataac cgttatatgt tgcctaaata
     8341 ggaatcaaac aatatcgaaa aaaggaggta tgacaaaagt cacacctcct tttttcgaat
     8401 aaacggtatt caaatcagaa cactgatttc acaaccactt tatttcaggt cgttttcaat
     8461 cacctttacc gcgtaatcat ttccgttgaa acgcgctatt ttttttgcgt ccatatatct
     8521 ttctaaataa tcatcttctc ctaattgctt atggcaagaa gcaatttcat aaagcaagta
     8581 ttccaatgca taagtcgatt tatgtttctt attatactca atacccttct cacataattg
     8641 gattgcttta tcataatcct cgattttacg gtaataggtg gcgatatcat aatatacaat
     8701 gcagaccatc agattatcaa aatcagtctc actcaatatt ttaagtgtac gctccacata
     8761 aactttcgca aaatctaatt ctccttttga caagtagatc attgccacgt ttgcagctgc
     8821 ttgtaagtga tggatatctg tttcggtcgt ttgttccaac acttgattaa aataaaataa
     8881 tgctgcatct ggttttttcg cataaagatg atcaagcagt ccttgataaa ataaaatttg
     8941 atttaaaaga tcagaattac ttactttttc ctgatcgatc gtttgtaaaa ttttataaga
     9001 ttcttggtat tttgatacag aattcaatga ttgaatctca tttatttttt tcctgatttt
     9061 gtcttcttct gtttctacta atatatcgga gatctcgaca ttcagctttt tgcaaagcgc
     9121 acttaagata ttgatgcctg gcgacatatt cttgttttcg atattgctga cagttgcttg
     9181 ttcacaaatg ccttctgcta attgtacttg tgttaacccc tttttctttc ggtattcttt
     9241 tagtaatgct ccatctactt taatcaaaac aatttccttc cctttaaaaa attatttctc
     9301 ctttatatca tacacaaaaa aaagttctaa acaaccaaaa atgtttagaa cagaataaaa
     9361 attttaattt taaaataata ttcaaattaa acaactggcg tatctgctcc ttgatttctt
     9421 tttgtgttat ctgaataatt atcgtttaca gttataacac atagtgaaaa ttgtcatggc
     9481 gagaaagtct aagaatattt ttaaacataa ctatttcttt cttacgtgtg tataatgacg
     9541 acagcacacc tatcaattaa tataattatt ttattttttt atgttctcat gtttattttt
     9601 aatgacacaa cttctcgtat ttatctggca caacatataa atcgatcaaa aaattgttgc
     9661 gtgttttttt gaaaattgga gtttcaactt gattacaaaa tttaaattta gaagttgtga
     9721 aaacagcgag agacagttta atattcaaaa tatacatagg ctactcgaac gctgtgtttt
     9781 ttagtatact agtaagaaac gaagggatag actcattcca ataaggagga tttcatgaag
     9841 acaattcttt ttgctcctgc agtattcaat ttagctgaga caacacgaat gattgaggtt
     9901 gccaaagcta tgaaagaccg ttattactgt gtattctttg gcttttctaa aacttttgct
     9961 cctttgatca cagaaagtgg ctttgagtat catttactag cgccgatcct tacgaaaaaa
    10021 caagaagaac aaatcataaa atttgaccag ttgcgtacac ttaaaaaccc ttttaccaaa
    10081 gaaatggttg cctctcgtat cactagtgaa cagagtctga ttgctaagtt tgagcctcga
    10141 ctgattgtga caggttcgaa tgtgacaatt tttttatcgg cccgcaataa aaagattcct
    10201 ttagtatatg ttaaacccta tgctttgagt gaaccttttt ttcaatcaga gcatgcgctg
    10261 atgccccacg agttacgttt cttcggccct ttaagcaatc ccctttggca ctggacaaaa
    10321 aagcgtatag taaatacccg atggatcccc aatgccttca aacacgctgc acaaaaagaa
    10381 atgcttcctt taccaaaaac aagtttacaa atgatggatg ctgatctaaa tctcatcaca
    10441 acgcctaaac tatttttgga agaagaacag ctgccggaaa attatcgaat cgttggtcca
    10501 atttttgcta agctcagtac ttccttgcct gaagaagtgc taacttttat acagcgaaag
    10561 caacaagaaa gaaaacgtat tatctatttc gcaatgggta gttcaggcaa tccgcgtatg
    10621 ttacgcaaaa ttttgttctt cttacaaaaa caaccggatc tagcggttat tgcacctatt
    10681 gactatctgc ttgatgacat tcaatctgac tcagaagatc ttttaataac agaatatctg
    10741 tctagtcatc ttttgcatga attcattgat ttttctttta tccacggcgg ggaagggacc
    10801 gtccaaactg cctgtgaatc cggcaaaccg tttatcggtg tggggatgca atttgagcaa
    10861 agctgtaata ttgactattg tgtaaaatac ggaaacgcaa tcgccctcac aaaaccagtg
    10921 actgaaagaa aactgcagat tgctctgcgc gaagtttctt cacccaagat tcgtcagcag
    10981 gcacgcacgc tgcaagccgt ttttccaata aatggtccac aacaagcaat tcaagaaatc
    11041 gaaaccttcc tgagcaacca cccaatatag tttcaagtag aaaaagcttt tttaatagag
    11101 actcatcagt aatttcgcta ttcgagaccc agacagtgaa aatacaaaaa aagtaaatag
    11161 tggattgaaa agttagagtt acttaaatta cggtgtctaa aaaacacaaa atcatcctta
    11221 aaataaaaga tctgacgcta tccaaatgag cataggtagc gtctgccatt tattcttagt
    11281 caaattcttt gacacctttc tctggttgga tcatctcaac gtttcttcaa aagaaagacc
    11341 ataataatta ggcaaataca ttttcttaaa atgtgtcata tagatctaga agcatcgctt
    11401 tgtagagatt tgtgccgcta gtcccctcaa caattactaa atcaaatgta ggatttacca
    11461 ctttatcttt gataatcaac ctccctcttc tgttgagaca atgatctcgc aatccgatgc
    11521 atactcctat ataaactgcc agcaatcccc acatattgag tagatcatgt cagaatgttc
    11581 tatgtcgtca gcaacgatac actaaaagtc acgttcacca tgactgattg ctattcccag
    11641 cacaattatt tctatacacg tttcatgatt tgaataaaca ctcacatcat acatattacc
    11701 attcatacac catactgcag cccctactat atggttgtaa gctcccaaat ctatatttga
    11761 cttgatcact ttggtagtag atctactaat tctaaatcca ttgctgataa aatttccatt
    11821 ttttcctcct tctttctgcc aaaacagtta aaacaataat aatctttatg taaaaaaggg
    11881 ctgtgacaaa agccttgtca cagtcccata ttccgaataa acggtggtca aaagcttgca
    11941 tctgcggtaa taagttcaac caaacgaaaa ataagccaac atttcacaaa aatttaagaa
    12001 gcaattttcg taaatttctt cttatttctt gaagctaacc acttctgtta caaccacttt
    12061 tagaacattt aaagcttcga tgtgacaaac catctatgct gctcgatcat aaaattgata
    12121 gcaaaaattc cacaaagtac atatgtcccc catgaaaaca aaacattcat atgcggtaca
    12181 atgatctttg gaagaatcac gatcaaaaat gatgccagtg cccaaatcca tcgattgatt
    12241 tgaatatgat atgtagctag catattcaat ataaggtgac tagccgttaa taaaaagata
    12301 aaaacaaaaa ataaaatcag ccaatacatt gtttcccacc ttctaaaaac caaacttaga
    12361 cagtctggac ttcgttttct ccacgacttt gttcatctga cagcatcttt ttatcataag
    12421 cttttacaaa tggataatag ataataaatg ctatcaatgt gttgatcatg atcaagataa
    12481 caacacgcca gtcatttcct gctgaaagat aaccgccgat tggagctggt aatgtccatg
    12541 ttggcaaaac agtaaatcta ttgaccattt ctaatttgac tgcaaaataa ctgataatag
    12601 tcaaaactaa aggaactaag ttgaacggga tgaacatgta aggattcaac atcataggag
    12661 ctccaaaaat tatgggctca ttgatattga agataccagg aataatagaa aaacgaccaa
    12721 cctctttcaa gaattttgat ttacagaaga atataaacaa aacagatacc acgatcgttg
    12781 ctcctgcacc accaattgtt accgtccatt gataaaactg ttcaggagcg atatagggta
    12841 attgggtggc agctgtacca tctgccaatg cctttgcatt ctcatccaac atgatcagcc
    12901 acattggacg aacaacagcc cctacaatac tcatgccatg gataccaaat gaccacaaca
    12961 aatggatcaa aaacattggt agtaaaacac caagcaaatt attccctaaa atattggtta
    13021 aaggctcgaa taacgataat aaggcagcgt ttacatcaaa gcccgcgacg cttcgaatca
    13081 gccaagtaat tgttaaaatg actgcacctg gaatcaatgc ctcaaaacta cgggcaacag
    13141 caggtggcac ttgttctggc attttgatag taatatttcg atttttaaag aaacgatata
    13201 cttccactgc aaaaatcata gataagatcc ccgcaaacat cccagatgct cccaaagaac
    13261 tcataggtaa gacccagcca agaggtgcct cttcaggagt taaaacgttt acaggaactt
    13321 gtaacataaa gaacgttcct aaagataata taccacctgt cacactatct aatccatatg
    13381 atttagctag atccgaaccc ataacaaaag ttgcataaat agccatcaat cccatcgtca
    13441 tcctaaaggg taaaacaatc gttgccgaat aaggggcaat aaattcattc catccaggaa
    13501 acggtaaatt gataaagacc ataaaaaatg tcccaatcaa aatcaatgac aatgttgaaa
    13561 caaccccatt acgaattgct aataaatgac gctgtgtacc aatttttgcc attggtggga
    13621 caagtttctc ttcgacccat ttcatcagct tttccactaa aaccactcct tttcaataaa
    13681 atatgaacca ttcattgcat gacaacgcaa agaatgttca taactaatta tataattaag
    13741 aaattttatt tcaatatatt ctcttaaata atcaaattta tttttaagaa tc
//
LOCUS       DASJTC010000073        13518 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.073, whole genome shotgun sequence.
ACCESSION   DASJTC010000073 DASJTC010000000
VERSION     DASJTC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.073"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(40..987)
                     /locus_tag="U4B19_002026"
     CDS             complement(40..987)
                     /locus_tag="U4B19_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEN2077754.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            1177..1446
                     /gene="rpsN"
                     /locus_tag="U4B19_002027"
     CDS             1177..1446
                     /gene="rpsN"
                     /locus_tag="U4B19_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEN2077755.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(1622..1984)
                     /locus_tag="U4B19_002028"
     CDS             complement(1622..1984)
                     /locus_tag="U4B19_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2077756.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(1995..3116)
                     /locus_tag="U4B19_002029"
     CDS             complement(1995..3116)
                     /locus_tag="U4B19_002029"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEN2077757.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(3132..3482)
                     /locus_tag="U4B19_002030"
     CDS             complement(3132..3482)
                     /locus_tag="U4B19_002030"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEN2077758.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(3612..5123)
                     /locus_tag="U4B19_002031"
     CDS             complement(3612..5123)
                     /locus_tag="U4B19_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEN2077759.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(5445..6797)
                     /locus_tag="U4B19_002032"
     CDS             complement(5445..6797)
                     /locus_tag="U4B19_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEN2077760.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(6950..8026)
                     /locus_tag="U4B19_002033"
     CDS             complement(6950..8026)
                     /locus_tag="U4B19_002033"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEN2077761.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            8222..9967
                     /locus_tag="U4B19_002034"
     CDS             8222..9967
                     /locus_tag="U4B19_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEN2077762.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(10247..10684)
                     /locus_tag="U4B19_002035"
     CDS             complement(10247..10684)
                     /locus_tag="U4B19_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEN2077763.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(10668..12041)
                     /locus_tag="U4B19_002036"
     CDS             complement(10668..12041)
                     /locus_tag="U4B19_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077764.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(12363..12851)
                     /locus_tag="U4B19_002037"
     CDS             complement(12363..12851)
                     /locus_tag="U4B19_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2077765.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(12873..13475)
                     /locus_tag="U4B19_002038"
     CDS             complement(12873..13475)
                     /locus_tag="U4B19_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEN2077766.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         3867 a         2933 c         2453 g         4265 t
ORIGIN      
        1 aaatgtttca gcttttttcg ttttttattc ctgtatttct tatttttcga atgacaggat
       61 gattttccca gcaggaggat gtccttcaac tagctgatgt gcttggatga catgcttaag
      121 tgaagcagac agattcattg aaataaaaac atgaaaggct cctttttggt aggctttgat
      181 tatgccagca aaggcttctg catctagata ttcctttctt ggaacaaacg attcataaaa
      241 gccttccttt ttttgccgca aatctagatc tgggaggtca tttaatgcaa catacctgcc
      301 gcctggtttc atgatctgga tgccggtttc tcctttgata cttcctttcg tcgcatcgat
      361 tacaaggtct gcctgatcag caaattgcag acctggattt gttttatcat aagcagcaaa
      421 tgctgaagct cctaattttt ttaccttttc ctcattttta ctggatgcac tggttaagat
      481 tcggattccc ttttcatgga gaagctggat cagacttgac ccgaccgcac ctgatgctcc
      541 tagtatcatt acagtatctg tcggctggat ttccgtcaaa tggttgatta gattataagc
      601 cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa ctcattttat ctggaatttt
      661 tgctactttt gctgatggca acacgacttc ttctccgtaa gtcccgccga ctgcatggac
      721 agctactcga tcacctacat gaacagttgt gacatctgaa ccaatctctg tcacaatacc
      781 agcgccatca ttacctggta cataagggaa tttcaatgtt cgtatctctt tcatttctcc
      841 taaacgcaag gcaacatcat aaggattcac actgaatgct tttatttcta cacgaacatg
      901 tccagaaccg acttctctgg gatgagcatc gatttcttca aaaacatctt gtgcttctcc
      961 gtaaccgtta attgcaaatc ttcgcatggt caccatctcc ctttttcttt aagtttacgt
     1021 ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt ggcgcatgat tcaaaacaaa
     1081 atagttgtct tttttcgcgt aacgtgttaa attatttatc taaaagcgta aagattacga
     1141 tttacgctaa taaaaaataa ttggaggaat ttgttgatgg ctaaaaaatc aaagattgcc
     1201 aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg ctttacgcca ggaattaaaa
     1261 gcgcaaaaag attacgaagg cttgcgtaaa cttcctttag atgctcgtcc agaacgattg
     1321 aaaaatcgtg atttaatcga tggacgccct cgcggataca tgcgtaaatt cgggatgtcc
     1381 cgtataaaat tccgtgaatt agcacataaa ggtttgattc caggagtaaa aaaagccagc
     1441 tggtaatcca agtctggctg taatgtaaag gaggagggcg catggaccac tccttggatc
     1501 atcaaaaaag atacgttcgc ttcttctagc agaaatgctg gtactagaag cagacgtatc
     1561 tttttttacg cacagctggt ccttttttat atgtaaaggc tgcatggtta cttcgctata
     1621 tttatttagt aacaggagtc agtgcttcga tacccacact aattttcaat gctcggtcaa
     1681 cttctgccat aatgggtcga tccaaatggc agaccttttc tttcaatctt gatttatcga
     1741 tcgttctgat ctgctccaac aggataacgg agtctctttc tattcctgtt ccagtagctt
     1801 tgatcccaat gtgagtcgga agcttaggtt tagccatttt tgccgttatg gccgcaacaa
     1861 tgatcgtcgg gctaaaatgg ttacctagat tattttgaat aaccaaaact gggcgtgttc
     1921 cgccttgctc cgatcccacg actggagaaa gatcagcaaa ataaatatct cctcttttta
     1981 ccataggatc ctccttattc ttgatattct cttggtatcc gctgtcctaa tccgcaagcc
     2041 acttcatagt ggatcgtacc taattgatct gctatatcct gcatagtgat ttctttgtct
     2101 gcatttttgc cgataagcgt cacttttgtt ccgacaggaa attcttgagg caatcggatc
     2161 atcagttgat ccatacatac acgcccaatc gtttcacagt agttcccttc taccaataga
     2221 gaaaaaccct gcatctttcg tggccagcca tctgcataac cgatcggtat cgttccgatc
     2281 cactctgctt caggagtgat atatgtttct ccatagccga ttccttcacc tgctgggagt
     2341 tttttgactt gaatcaattc agaaaccagc tcaagtgctg gctgcaatgg ataaacttcc
     2401 ggtagtgcgt gcccagaggg attaagtccg tacattgcta ctccataacg aatcatattg
     2461 ccgattgttt catcatgcca taaagccgtt gcactattgc tgacatgtac atatcgagga
     2521 agttcgggaa gttttttcag cacttctata aagcgttctt tttgcagatt ccaatatgta
     2581 tcatctgcct gatctgctgt agaaaaatgg gtaaataaac cttcccattc taatacacga
     2641 ctttctttga tgaatgcagc tgcctctttt acttcttcag gtgtacaaaa tccgatacga
     2701 cccatcccag tgtctgcttt tagatgaatc gaaagaggcg tctctagctt acttttctct
     2761 aaaacagcag ctgctgcttg caaccattcc cgtgtacctg cagttactga taaatcatga
     2821 gcaacagcta cagtcagtgc gtcgtaagga accatattca agataaggat gggctgagta
     2881 aatccagctt ctctcaactc gactccttca tctaaatttg aaacacaaaa gcccgaagct
     2941 ccacctgcaa ctgctgcctc agctgtttca atagctccat gaccgtatcc gttcgctttt
     3001 actactgcaa acaattcttt tccttgcgga agtcttgcaa cttcatttgc tacattttca
     3061 gttatagctt ttttatgtat cactgctttc gttgggcggt gccaagcgac aaccatcgtt
     3121 tttccttctt tctagtcttc caaaataatt tgtgcaaaag ctgtttcatt tgtatgagta
     3181 atactgaccc atacattgcc ttcatgaggt gaacgcgtca caacaggctg accattttca
     3241 ttttttaaaa tctcgacatc ttgaaaggta acttttccga tcccagttcc ccaagcttta
     3301 gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt ctacttggcg atgaaaaggc
     3361 aaggactgga atagtttcat ttcatcagag gtcaagattc gtgcaatgaa ttttggtttt
     3421 tcttcgatca gccgagtgat cctaggaagt tcgactgcat cgatcccaat tccttttatc
     3481 atatgctttt ctcttttcaa taaatttaga acactctcat tctaccaaat tataaccatt
     3541 ttgccacaaa taagtccaca aataacaaaa atgaaagttt ctcttaaaaa agagaaactt
     3601 tcattttatg tttaatcgtt atttgaacgg attacaaatc cgcgtttttt atcattgtga
     3661 cgtttaccgc catctttttg gctgttatta tttttgctat aaccgccttt tgatttgttt
     3721 ccacgataat taccgccgtc acgacgatta cggttgtttc cgccaccacg tttgccattt
     3781 ttattgtatc cttttttctg cattggcaat gggcgttctg gtgtgatctt cactggcact
     3841 gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg caactaaatc ttgtgcagaa
     3901 tattcttcta ataatttatc tgctgtttgc aggtatttat ctagaccatt ttctgcaagt
     3961 tttgtttcga tttgttcaac tgcagcacct aattgacctt tgaatgcttc tttttcggtt
     4021 ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt caatcacatg caagtagtcc
     4081 atttcatttg gtgtaacgaa agtcacagac ataccgcctt ttccggcacg gcctgtacgt
     4141 ccgatacggt gaacgtagct ttctggatct tgagggatat catagttgta cacatgagtg
     4201 acacctgaaa tatctaatcc acgtgcagca acgtctgtcg ctacaaggat atctaaatga
     4261 ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt gagacaaatc gccatggatt
     4321 ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca attcgtctac acggcgtttt
     4381 gtacgtccga agacgattgt taattcaggt gtttgaacat caaacaagcg agtcatgata
     4441 tcgaattttt cgtattcttt tgcgcggaca tagtattgat cgatcaagtc agctgtcatt
     4501 tctttcgctt tgatcttcac atgatgtgga tttttcatga atttaacgcc aatattcttg
     4561 atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc tttgatcagg aacttgtgaa
     4621 atgatttttt cgatatcttc caagaagccc atattcaaca tttcatctgc ttcatccaaa
     4681 actaatgttt gaacggttcc aagtttcaat gtgtggcggt taatgtggtc aagcatacgt
     4741 cctggtgtac ccacaacgat atgtggacga tctttcaagc cacggatctg acggccaata
     4801 tctgctccgc cgtaaacggc ttgtacacgt acttttttgt cgcgccctaa acgatataat
     4861 tcttcttgtg tttggatagc taattcacgt gttggtgcga taactaaacc ttgtaattcg
     4921 tgacgatctg gatcgatttt ttctaacatt ggtaatccaa atgcagctgt tttccctgta
     4981 cctgtctgtg cttgtccaat aacgtctttt ccagctaatg ctagtggaat cgtttctgct
     5041 tggataggcg ttgcttcttc aaaaccagcg cgttcgactg atttcaacaa ttctggtgat
     5101 aattctagtt ctttaaattt caaatgaatc ctcctatatg gttgttaact gaggatgtag
     5161 cttttttgct accccgtcga tttgttcggt caaagtgtat tgtttctttt tgacagtggc
     5221 cacgcacgtt tttatccatt cttagcccct gtggcaccac cgaaaaatca ctttgcgaag
     5281 tttaggcatt agaaaagcca aacgggtcgc gttcagcttt atcattatta tgacatctgg
     5341 cttcaacaag ttgtttctat ggcaaaacca gtccaatttc tcttcatctt tcggcaatag
     5401 gttattctaa catagttttt cgttttctgc aagtttacag atttttattt catttgctga
     5461 agttgttcta ccacttcaaa taagcccatg ctgttgctgc cttttagcat aatactatct
     5521 gttgcttcaa gattttgttt gatagcatca atcaatttat tcttttcttt ttcaaaatag
     5581 aacacaggta gttgggggta tttttggttc aattcttctt ttagtgcatg catttgttcc
     5641 ccataaagga aaatcatggc gaatttctca ggatcgatat gttcactcat ttgcgcatgc
     5701 atatttaaag actctttccc taattccagc atatctgcta acacagcgat tctgcgtcct
     5761 tttgttggca gatttttaaa agtatctaat accagtccca tggctgttgg atttgcattg
     5821 tagacatcgc tcaaaatagc tgcaccattt cctgccgtta gccattcggt tctgttttga
     5881 gtgatagaaa catgggcgag cccttggcgt atctcaggta tggacagtcc aaagaagcgc
     5941 ccgatcgtat acgcaatcaa agcattcgtt acattataac ttccaggcag cgggatcgta
     6001 aatgtttctc cttctactac aaaatcggtt tgtgcttttc cttttgctgt cacttccgca
     6061 tgaacatcgc catttccaat accgaatgtt gtgactgttt gtactaatgt tgccgttaaa
     6121 ggtgtcagca acggttcatc tgcgggcaca acgagtaatc cgtctgatac aagtccgtca
     6181 gtgatttcca ttttggcttt agcgattcct tctcgcgacc caagattttc tatgtgtgct
     6241 tcaccgatca ttgtgattgc tgcagctttt ggttgagcca taagtgaaag ttccgtgatt
     6301 tcattggcgt gatccatgcc catttccaaa acgatctttt ctgtatcttg gggcatgtgc
     6361 aaaatcgtgt aaggaagacc aatattattg ttgtaatttc cttgcgtctt atatgttttg
     6421 tatttttcag acaagacaga cgcggttaga tctttcgtgg tggttttacc attgcttcct
     6481 gtaattgcaa cgacttctgc gcctactttt tccaagtaat aggttgccaa ttgctgcatg
     6541 gctttcaaag gatctgctac aggaataact ggaaaatttt ttggagcttg ttcaacaggc
     6601 aagctccaaa atgaagcaga tgctccgttt gtgatcgctt gctggataaa ttcatgtcca
     6661 tctcgcgtac cagcaagagg aacgaataaa cttccttcgg ttatcttccg gctgtcaaat
     6721 tcgacattgg taatctttgt cagtgcagag cattcgctgc ccacggcttg tgcaatttct
     6781 tgtattgtta gattcattga gatctctcct aaaaaaggtt gtgaaaaaag cgtttggcct
     6841 aaacggcggg ttcgcatgac tctcacttcc aagtaaattt ctctattcgt atttagaagc
     6901 tagaatgaac gcggttacgc aatcactcca gcctcttctt tatttctctt tattcatttt
     6961 tttcaaaaaa agattgacgc tgatggtatc gattcattcc taactggatc agttcttcaa
     7021 tcaaatcacc gtatttcaag cccatatttt cccataagag tgggtacata ctgaactccg
     7081 taaatcctgg catagagttt aattcattca ggaataattc atttttattt gtcaaaaaga
     7141 aatcgcaccg gctcaatccg cttccaccta acatcgtgta agctaacttc gcgtactctt
     7201 gcgctttttg ataaacttct tctggcactt cggctggaat ctgcatttcg attttattat
     7261 tgatatattt tgcttcataa tcatagaatg ctacgtcttt tacgacttca ccaggcaaag
     7321 tcgtccgaac gtcttcattt cctaatacag caacttcgat ttcgcgcgct tcaattcctt
     7381 gttcaacgat tgctcgagaa tcatactgat aggctgttgc taaagcattt tgcagctctt
     7441 ctcggttttc tgcttttgta atgccgacac tagaacccat attcgccggt ttgacaaaca
     7501 tcggataaag caaagaacct tcacattgat caaatacttt tttaggattt tctttccatt
     7561 gattcttaag tactggtaca taaggaactt gcggcacacc agcagcttgt aaaatatact
     7621 tggtcatgat tttatccatg gcacatgcac tggtcaatac gcctgcgccg acataaggca
     7681 tattcaatgt ctctaagaag ccttggatcg ttccatcttc cccgtttggc ccatgtaaaa
     7741 ctggaaaaac gatggctcct tcttctttga tttcgcccgg attgatcact tttcctgtaa
     7801 agccttcctc tgtctgtcca cttgggtccc atgaaagatg caagacatct ttgctggcag
     7861 gtttttctgt tagtaatgga cctttgaccc attgtccttc ttttgtaata aatacgagtt
     7921 gaacttggta ataattataa taaatggcat ttaaaactga aaatgcggaa agaatggaca
     7981 cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat cttcaaaaga ttgtcctcct
     8041 gtattctaaa tcgttcagtg atttactctc atattttagc acaaaacaaa aataaacgaa
     8101 taacttattt ataaaaaaac gagccagctt ctaaaagaac gagcgtcatt tattttttgt
     8161 cagctagatt atttgtcccg ttttttataa aaagttatga taaagaaaag gagggatgat
     8221 gatggcgcaa acaaatccac gcagccgctt ggcagaaatc attcgagtgt tcatcaatta
     8281 caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa caggtaaaaa aagcctttga
     8341 agaactaggc cccaccttca tcaagatagg acagatgctt tctgttcgag aagatttgct
     8401 ttcttctgcc ttcactcaaa cattcaaaac attacaagat agtgtaccta gcgacacatt
     8461 ttcaacggtc aaaaagacga tcgaaacaga actttctctt tcattatcag atatattcga
     8521 tgatttttca aaatcgcctt ttgcctctgc ttctatgggg caggctcatc gtgccaagct
     8581 gaagaacggt gacgcggtag tcgttaagat ccaacaccct aacatcgccg aagagatccg
     8641 acttgatctt caactatttg aacgagcgat ccctttgatc aaatacattc ctgaaactag
     8701 cgtagttgat ctaaaagggg tactccaaga agtaaaacgt tccttgatca atgaaatgga
     8761 cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa aagaacaatg gctggaaaga
     8821 gatccgcagc cccaagattt atgacgcttt ttgttcgaaa aaagtgatcg tgatggaaga
     8881 aatgtctggt aaaaacttga atcatttaat gaacgccgaa aacaaaactg aaacttttat
     8941 cactggaatt caaaacaagc agctgaaaca agaagttgca aagcttttag ttgagaactt
     9001 tatgaaacag gtgtttgatg atggtttttt ccatgctgat cctcatccag ggaatctgct
     9061 attccatgta ctaacaaaag aagaacagac acaagctagc cgaaaaactg aaacagtcca
     9121 tgaaaaagag ttcggttcct ttgcttttcg agcctccact tctgcagaag accctgttgc
     9181 gccatatacc atcaactaca tcgattttgg tatgatgggg catctttctg caggacttag
     9241 gcaaaaactc acacaagccg ttttagcgct gtatacgaaa gatgcttatc gaatcgaaaa
     9301 agcggtcctt cgactttgtc agcaagaagg ctcgtttgat gaaagtcggt tccaccaaga
     9361 actgacaagt tttcttgaac agtattatga ttctcctatt gatgaaatca acttgcaaga
     9421 agtattcaca catgtcgtga cgatttgtca tcaaaataat ctgcagttcg atcgtgatat
     9481 cacgcttttg ctgaaagcat ttgggacatt agaaggggtg atccgcgtcc tcgatcctga
     9541 agtttccctc atggaagtcg cttcgccttt tgctcaacat tattttctca ctcatctgga
     9601 tgtagaagat acattgaaac aaagcggatt agacttattg gaagggatga aggctgcgcc
     9661 taagatccct caacagctcc accatctatt agaaatgtgg acttcgggac aaggaaaagt
     9721 caatttggaa ttgaaaaagc aagacaaatt attgagcagg atcgaaagta tgatcaatcg
     9781 gcttgttttc ggtatgattt tagctgcatt gatcgtaggg tcctccctgc ttgtccaagc
     9841 agcacctgta gaaaacgcag aggtcgtttc cttattaggg atatttacgt atgctattgc
     9901 cgcattcgtc attatttttt tggcaatcga tgcattgatc caaatgtata agaagaggaa
     9961 aaagtagaaa aaggttgtga cagaaagaat cagcttcaag aaataagaag gggttttaca
    10021 taaattgttt ctcaaatttt tgtgaaaatt cggcttattt ttcgaagagg ctacttctga
    10081 aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc cagtaataag aacaatcaaa
    10141 aagggaacat ggaacacatt tatcctgttt ccctgttccc tttttgatgt attcgagcat
    10201 aaataaaata ccttcgttac ctactatctt ctgtacttgg taaaccttag tcaattgcct
    10261 tttttaacgc ttgacgaaaa gacatatgat ctttctccgt aaagggtttt ggaccctttg
    10321 tccttatgcc agcttttctt aactttccac tatgataacg ttgttctaga agcgtgccga
    10381 tattcgcttc gtcaaattct tttccagagt gatggaacac tcgcacaccc ttcggcaata
    10441 caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc attcttctta accataccga
    10501 cgattttata atccgcacta tcagctccgc ggtctacgta gacgatttgg acatgattag
    10561 cgtattcttt ggtcgtataa tggtccacac tggtcacgat aaccacgtca agaccatagg
    10621 attctcctaa ttttatgaca tcttctttta ctggagaacc atctccatca atgaatagcc
    10681 tcattctttt tcgccttctt ccataaatga taaacagtta ctaatccgct cagtaaaaga
    10741 ccaacattga tccattttgc caagataagc agttgtacac tttctatcgg cactgattca
    10801 tggttcagcg tgatcaataa tagatggatt ggctctccca tttcttttgc taaaacaaac
    10861 aaaagcgtca tcaataaaaa ctggtggatc acactccctt tattttgagt agtatacttg
    10921 acaacgatcc ggcgatccat tggaatagct gtatcagttt gataatattc cttatttagc
    10981 cagctgcctg ttactacgtg ccagaaacta agcagaaaga cacctatcgc aaaaccattt
    11041 ggaatcaaga ggaccaataa agaagaactc agtccaagca attgcatagc caatggagac
    11101 catgttaaac gtttcaaaat ccatggtccg actatttttg ctgcaatcaa gcctgctgca
    11161 taaggcaaat acataaaaat cgaaagtttt tctaatccgt acagtatgcc aacataaaaa
    11221 ctgctaaata aaaaaaggaa attccctagc attccattta gaaaagtaaa cagatttagc
    11281 cagcttggca acttccattc cttcttctgg tgtcctaaaa caaacacgaa caagatgaac
    11341 agcagcaaga aaccataaat agcaagatca aaaagccttt catcgaataa caatcgggca
    11401 tttctcaaaa gaaacaataa gccaaagaat atcaaaaata aaacaagctc tcgcgtagct
    11461 accagatttc ttttgatgtc tttgaaatcc agttcgtaac gcggataatg actaaccgta
    11521 tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc ttgctgcaat ctgagtcggt
    11581 gcaggcaact caatcgcttg atacaggaga aatagccaga tagctgcaaa taagtacttt
    11641 ttcccatcca tattgataaa accttgcgtc ttttcgtgaa aattgaccgt ggtattagca
    11701 ggtacaagcc atgctgcact cagccctagc aaaacacttg aaaaataaaa taaaggaaaa
    11761 taaaatacgc ctaatattcc aagcaatgaa ccagcaccac caatcagcag agcgatcatc
    11821 agcaacgtgt aactatctag accaaatcgt agactgttga ctaaaaatag tccagtcatc
    11881 cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc aattatgcga gtctgttagt
    11941 cccaaaaata aaagataagg aataaaaata accgtgttac ttaagataaa gggcgttgtt
    12001 tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca tgacaatggc tccttaaaca
    12061 gtcttataac taatctgctc ttcgatttta gcattaaagg ggaaataaac caattttaca
    12121 gaatatattc ttgtaaaaaa aaatacagaa agaagcataa gcttctttct gcatttttac
    12181 ggtacttgat tactcaagca gacaagccct tctccacaaa agtcttgccc gacacccctg
    12241 catccggcag gtgataaaga tagaaatcaa ttatcctgtg cctccaaaat caattaggag
    12301 ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt tactttaaca cagttcctat
    12361 gtttatgcga gagctttttt cattttataa tcgaccattt gaaatccttg accgatatct
    12421 gaaataagtt cttctgtaat ctcaaatcct tcatgcttat aaacctcgat tgcttgatga
    12481 ttcccctgat tcactcgcaa aaagatctct ctttcctttg tttgcgcagc aagttcttca
    12541 taccagcgaa aaactgaaga agtcaatcct tgaccacgta ctttatttag caaatataat
    12601 ttacttaaat aaatttttcc atttatctct tcataagcag tgtaaccgac agtcttttct
    12661 tcgcttttta ataaaaagta ggacacacct tctgtcagtt ctttttgaat ctgttttttt
    12721 gactgataag ttttcagcat gtaatccact tgtttttgac caatgatcgg cacaaaaact
    12781 tccggccata cttcttgtat gactgccatc agctcatcca gttcattttc tgttactttt
    12841 attttttcca tacacccacg tcctttttta cattataaat gaccttgcgc ttgatggaat
    12901 aaaaagagca tgatcatgct atacacaccc accgaccacg gtttcaagaa aatcacaatc
    12961 tgcataaatt ccctaaaaga taatttcgtc aaaccagcta ctagacagat caagtcgtct
    13021 ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt taatatcttt cgtttttctt
    13081 gtcagcaatt catcaaattt ctgatacttt tttggcgata aaataagctg gacaaaggct
    13141 acgccatagt aacggacaag caaaaatccg attgcttccc caatcactag gccaatacaa
    13201 ctatataaaa gcccaaatcc attgccaaac atcagcatgc ccgctactga ggagatccct
    13261 cccggcaaaa taggaatgat cacttggatc acttgcaaca gaacaaatat catcactgca
    13321 tacttgccaa attgctggat aaacgcttgc aaagacgtgc tcgatcgaaa aatcccatga
    13381 tgataagcat aaagaatcaa cacaaagaaa aaaaacagac caataatggg catgatctga
    13441 agacattttt gtaaaactga tcctttttta gacatctata ttccccacat cttttttcat
    13501 ttattaaaac tgcctcct
//
LOCUS       DASJTC010000074        13285 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.074, whole genome shotgun sequence.
ACCESSION   DASJTC010000074 DASJTC010000000
VERSION     DASJTC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.074"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(431..1045)
                     /locus_tag="U4B19_002039"
     CDS             complement(431..1045)
                     /locus_tag="U4B19_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2077767.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1651..2028)
                     /locus_tag="U4B19_002040"
     CDS             complement(1651..2028)
                     /locus_tag="U4B19_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077768.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2328..3209)
                     /gene="galU"
                     /locus_tag="U4B19_002041"
     CDS             complement(2328..3209)
                     /gene="galU"
                     /locus_tag="U4B19_002041"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEN2077769.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(3252..4271)
                     /locus_tag="U4B19_002042"
     CDS             complement(3252..4271)
                     /locus_tag="U4B19_002042"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEN2077770.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(4904..5908)
                     /locus_tag="U4B19_002043"
     CDS             complement(4904..5908)
                     /locus_tag="U4B19_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2077771.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(5921..6970)
                     /locus_tag="U4B19_002044"
     CDS             complement(5921..6970)
                     /locus_tag="U4B19_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2077772.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(6987..7808)
                     /locus_tag="U4B19_002045"
     CDS             complement(6987..7808)
                     /locus_tag="U4B19_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2077773.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(7795..8574)
                     /locus_tag="U4B19_002046"
     CDS             complement(7795..8574)
                     /locus_tag="U4B19_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2077774.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(8591..9061)
                     /locus_tag="U4B19_002047"
     CDS             complement(8591..9061)
                     /locus_tag="U4B19_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2077775.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(9073..9492)
                     /locus_tag="U4B19_002048"
     CDS             complement(9073..9492)
                     /locus_tag="U4B19_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEN2077776.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            complement(9573..12350)
                     /locus_tag="U4B19_002049"
     CDS             complement(9573..12350)
                     /locus_tag="U4B19_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2077777.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            12541..12765
                     /locus_tag="U4B19_002050"
     CDS             12541..12765
                     /locus_tag="U4B19_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077778.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
BASE COUNT         3912 a         2762 c         2155 g         4456 t
ORIGIN      
        1 ttttatttct attagaaaaa ggtatcctgt tggacaattt tgttatatca ggtaaacttc
       61 attgcaataa tgataacaaa tgtactataa ttactttgta aagagttttg gggaagactc
      121 tttcatcaac gtgtattaag agacaagaaa gaatccatat ggattctttc ttgtctcttt
      181 ttccactact ttttattttt ctgacactac atattcttat aactcatttc tcataactgt
      241 gaaacaactt ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc
      301 atcttaaaat aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt
      361 ctttttttca aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac
      421 atgtggataa ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa
      481 cttgctgcag caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat
      541 ttggcaaagt caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa
      601 attcaaataa tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc
      661 cagtaacaat gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg
      721 ccgggtttct tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct
      781 gatcaataat tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt
      841 caatcaatat acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac
      901 ttagtgtgcg gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc
      961 aagtgccagc ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat
     1021 aatagtcaaa ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata
     1081 gaaacagctg cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg
     1141 tggtctttgc tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca
     1201 tagcaaataa aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc
     1261 ctagattagg tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt
     1321 cagggtgtat gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga
     1381 gccttttttt attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg
     1441 caagcaagaa aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa
     1501 aaggaatggc ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt
     1561 gaaaaaagct tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt
     1621 ttatcttttt ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg
     1681 ttcgagagaa tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga
     1741 ctttcttttc atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa
     1801 tacgcaagct tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga
     1861 tgctgcatac cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat
     1921 aaatagacta ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca
     1981 cttgagtata tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca
     2041 ccttttttta taattaaaaa attcgccttt ttattctact atgaatcttc tggcttggct
     2101 ttcatttctc ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag
     2161 cataatattt tctgaatcta ttcacgaaag tctttttcta tctttcgttt tccacgactc
     2221 aactgatcta gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc
     2281 tttaataaca aatagccttt cattctcagc aggccactta tttccagtta actttctgtt
     2341 cttagctttt tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt
     2401 tgaagtccat attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt
     2461 tttcctttaa actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc
     2521 gttagctgta tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta
     2581 ggagtcagta gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt
     2641 ttttctacaa acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata
     2701 attccgtatt tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt
     2761 gtttcttcat aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca
     2821 tcccccagca tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca
     2881 tcccccagtc ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct
     2941 tcaactaaag cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata
     3001 tttgcatcaa agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct
     3061 tcgattcccg aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt
     3121 ggaagcattt cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg
     3181 gcaggaatga ctgctttttt gaccttcact tgctgttcct cctaaacgac gattaactat
     3241 tgatcatcta tttaacgaag agaaaattca ttctccattt ttccgtcacg taacatgatt
     3301 tcttttgctg cttttttgat atcttctcca ttatacaaaa cattatagat tgtttgagta
     3361 attggcatct caacacctag ctgttgagct agctctactg ctgcttttgt cgttgccaca
     3421 ccctcaacga ccattcccat gttttctaga acttcttcca gcttatgtcc ctgtcctaac
     3481 aagttaccgg cacgccaatt gcgagaatga acacttgtac aagtaacgat caagtccccc
     3541 acaccactca aaccgataaa tgtcagagga ttggcaccca tcgcgacacc aagacggctg
     3601 atttctgcca atcctcgagt catgattgct gcttttgcat catctccgaa tcctaatcca
     3661 tggatagcgc cggcaccaat tgcaatgata tttttcagtg ctgcaccagt ttctacgcca
     3721 atcacatcat tatttgtata aatcctgaaa taatcattca tgaacagttt ttgtacatag
     3781 gctgctgctt tttgatcaat acttgcagct gtaattgttg tgatatcatg tactgccact
     3841 tcctcagcat ggctaggacc agataataca acaattgcct gtcttttttc ttctggaatt
     3901 tcttcagcca aaacttctga gattcgttta tgcgtatctt gttctagtcc tttacttgca
     3961 tgtataatga ttggcttagt attcatttta ccaataagtt cctgtgcgac tgaacgaatc
     4021 gcttttgttg gaacaacaaa taaaatagca tctgcgttat ccactgcttc tgccaattct
     4081 ttatatccga taatcgattc tgggatcgtc aaatcaggta aaaaatgctg atttgtatga
     4141 tacttattga tttcatcaat ctgttcaggt acatttcccc aaatacggac atcatgcccg
     4201 ttttctgcca atgtttgtgc taaagcagtg ccccaagatc caggaccaag aactgcgatt
     4261 ttttgtttca tggatattcc tccaaataaa taattgataa tttccattat agtatacatt
     4321 tcttaaagtt gggactaatt tccattgttt ttaagtgttt gtttacttct tttatcgttt
     4381 ttacttgaaa taatcaatga tatgttacaa tagtctacat tttattttta cactcgttat
     4441 gctttgcttg ttcctaagag gaagcagttt tgacaatgtt actaccagca ttactattag
     4501 gaggttagga aacttctcct agtgagtatt gatttaaaat acataagaga cagattgaac
     4561 atttctaaat ctgtcaggtc ctgcaacgga cagattgtct ctgttgacgg ttgacatagg
     4621 aacttttgtt ttagtcaaca ttctggatac aaaacgctat gtaaactggc gcaactcgtt
     4681 gtacttggac ttttactacg gatccatgca acaactaaat agtactattg cataacaaaa
     4741 aatggttggc acccacatgt cccattaaac agattataag ttgaactgta gataatgcaa
     4801 aaaatgtgtt ttataacgta attgatgcca atattttaaa aaaataaagg gagcagttat
     4861 cgctgctctc tttatttttt atattctttt tatccagtct atcctaataa tcaaactgac
     4921 gatagtagcg gcgtatctct aatggatggc agttcaactg ctccacatga gcatcgatgc
     4981 gttgtgtcac tacatgcatc aataagaaag acaaaacacg tccgcgatat ttttcactga
     5041 taccttctac tggatagtct tttgtgtcta tcaatgtata attgctgcaa atttttggca
     5101 aaagtttagc cacacgttca gataacactc tctgttcatc ttcgcctaaa aataatgtga
     5161 ctggggttgt ttcttctacg atctctaacg tcccgtgaag aaactcggca gcatggatag
     5221 atttcgatgg aagccagctt tgttcttccc agtaacacat ggcatatgaa taaacagctc
     5281 cccattgatt ccctgctcca ataaaataat gcatactatc acgtcgatgt ttttctgcaa
     5341 atgataagcc aaatgcatct gcttttttct ctgcttccac taaccctgtt ggtaaataac
     5401 gttcgagctg ttcatagtat tctgaataag cttcgaattc tccattcaaa tacatcaatc
     5461 gatctgctgc cataaaaaat ttgatttgtt ctgttccagg agctggataa gtgaccacaa
     5521 aatcgcatag ctgagacaac ttactgttcg ctttatcgat aaatccaatc agtgtcgctc
     5581 ctactttctt gatttgttct actgctttca ctacttcttg agttgtaccg gtaacagagg
     5641 acaaaataac gatagaatct tttgttaatc ttttgtttcc agtagtgtaa tattctgcag
     5701 catgctgtac aaagacaggc aagttgcttt tcccattcat ataagtgaca gcttgcatcg
     5761 aggaagcata cgttccaccg attcctagat aatagacagc gtcaaatttt tccgcataaa
     5821 gtttatcgat gatttcttct acttgcggac gtaaagctag tgcgcctttt attgctttga
     5881 tttgttcttc ttcattgaat ttcagcatga tttccacctc ttataattta cttttcaaga
     5941 ctgtatcaaa gtcttcaaaa atctttttgt tcaagtcaat acctttagct actgcggttt
     6001 tataagcgaa tagttgaaac ggaacgacaa gcgctaatac agacagatat tcatcacatt
     6061 ctattgctaa tccaagtgtt ttctcgtttc ttgctttttt tgtggtaatg gtgagagtct
     6121 ctccaacgta ccgactcata tatctttgca acttttgcga acgttcctta tttacacttt
     6181 ccccttcaat aaagaatagc aaatgcgaag catctgcctc caggtatggg ccgtgcatat
     6241 aagcttctaa ctcaaatcct tgtgacggaa cgcggattgt ttcagtcaat ttcgtctctg
     6301 cttctttagc tgttccccag tttggaccat atccaatggt gacgaaacgc tgtgctaaac
     6361 gaaataaact ttgatatcca tcaaaaaatt cttccgtttt ttgaataatc gctggtaaat
     6421 ggttgattat tttgcgcaac tgttgcatgt agtcttgttc tatttttttg ctgattttat
     6481 ttttacttat tccaattcct atccctaaga gtagtaactg taaaacagtc gcagaatagc
     6541 cttttgtaac gaagcctact gattctaccc cggcacctaa atcaataatt tgtgcagctt
     6601 ctaaagcaag tggactttgc acgtcatttg tgatagcaac aacaggtaac ccttgttttt
     6661 gtatcatttt tactgcttca acagtagaag cacttttccc gctttgtgac acagcaatca
     6721 cagtatcgat tgaaggagaa agttttccat aattgaaaaa ataaaaaggt tcttgaatat
     6781 cgacagtaag atcaccataa gattccaatg ctggcttcgc tgctaaacaa gcgttataag
     6841 atgaacccgt tgctaagatc agtaaattca ctgttttttt catatgttct cttgtcgaaa
     6901 aatcattctt tttcaagatc gtacttagta cttccttttc ttcatagata taatcttgca
     6961 tcgtaaacat tttctctgct ccttttttaa aaaatcccta tccacgaacc aaaaattcca
     7021 aagaaagcta gtccgatcaa gatcgttaat ggtttcactt ctttcttcaa tagataaaac
     7081 actatgccaa atgctccaag acataagata tttggaacga tatcatcaaa aatactttga
     7141 acagtcacag cattttctcc tgaaccgatt tttaaaggaa tattgatggt gatcatcgtg
     7201 gcagtcattg ctccaacgac catcagtcca ataatagacg cgcctaatgt caaactttcc
     7261 atcataccgt ttgcctggat ctttttcaaa aaacccgttc ctaagcgata tccccaacct
     7321 gttgctagat agcgaaacag aatgtgcgga atattgaaaa tcaaaatgaa caagataggg
     7381 cctaaaatat ttccttgcat ggctaaagag gtacccacac ctgtggcaat caatcgcaaa
     7441 gttccccaaa agaaagaatc tcctaaccct gcaagaggtc ccatcaaaga tgttttgata
     7501 ctatcgatcg cagtcacatc aaattcagga tcatccttat tttcttcctc cattgcagcc
     7561 gtgattccta aaatcagtgt aacgatatgt ggggtcgtgt taaaaaactc tagatgccgt
     7621 ttcaaagctg ctgccatttc ttctttcgac tgatataact ttttcaaaat cggaatcatt
     7681 gcataaacat aagccatatt catctgacgt tcataattcc aagaaaactc catttgaaaa
     7741 gaacgccaaa atacgtgatt tagctctttt ttcgttattt tgctgttttt gatctcagaa
     7801 ttcttcatct tcttctacct cccctgcagt tgtttgaagt ggtagttgac ggacattcat
     7861 aatattgact accacaactg ctaaaatcgc accaaaaata gctactccgg tcacaggaat
     7921 ctctaagtaa gaagctatag caaaacctaa gaaaaaatat ggtgcaactt gtttgttgat
     7981 cagcaaacga gcaagcatcg caaaaccaag agctggaatc aaccctgtag ctactgacaa
     8041 ccccttttga acgaagtcag gaatcatatt taatagattt ccgatcgtat tgcttcccac
     8101 catataagaa accattacca caacaccaag catcaaggaa agaccgatac cggcaagcaa
     8161 gtgcatatgc tcgactcctt tataatttcc ttcttctgca taattgtctg ctttatggtt
     8221 cataattgga atcaaaagcc ctaagtaaat atttttcaat atcaatgtca acgtagcaat
     8281 cggcaaacct agtaaaagcg cggtttctgt accggctcca gctgtaatag caaaagccgt
     8341 ccctaaaatc ccaccggtca caacatcagg tggaatcgaa gcacctactg agaacgatcc
     8401 gataaatgct aactctaaag ttgcgcccat aatgatccca gtttttacat cacctaacac
     8461 gattcctgta aacagccccg tcacaatcgg acgagaaagc aacgatgtcc ctagtgcata
     8521 ttctgattgt gcgacaaatg caacaattcc taataagact gcttgtacaa tcattgaggt
     8581 tcttcctttc ttacaaaact tctcttacat ccactttttt atctgctggt acttgacgga
     8641 tttctacttc tatttgtttg tcaattaatc gtttcaagcg ttcctcctct tctggtaaaa
     8701 tatttaccgc ttttccgatg ctgcgagtac caggttttgc cttgatccct cctaagttga
     8761 tggttttgat ttctggatac gcttctgcca gcttatatgc atcctcgatc gattccacta
     8821 cgatgaataa cttatattta tctgtcaccc cgcttttcaa cgcggcaatc gagtcatcaa
     8881 ttgatttgat cactagtttg actccttgtg gcttggctaa tttgatcgtt gtctttcgta
     8941 tctcattcgt aggtacatca tcattagcaa tcaaaataca gtctgcgcct attgtttgtg
     9001 tccatgaaaa agctacttgt ccgtgtaata accgatggtc gactcttgtc aatacaatca
     9061 tttttcttcc tcctaaaatt cgtcttcttc tattttctga ttgagccgct cgttacaaaa
     9121 ctggatcatt ttttttgaat cagttagcac ctgctggatc aaaacagttg tctctacttc
     9181 agcgtccaac cgagatacta gctcaatcaa tagtggcaaa ttcaatccgg caacgagata
     9241 gacatgatcc cgattgatat attgtaagaa ttcgttgttg acacttccgc caaaaagatc
     9301 cgtcagaaca atgatttctt tatctttgta cctcataaaa agctgcctta ctgtttccgc
     9361 taaatcaaaa ttctcatcag tatagcaatc gaatacagta atagaatgat ttttcccata
     9421 gatcagctct aatgaatcta atatccctga agcaaatttt ccatgggaag ctaagataat
     9481 ttctctatcc actttttcat ctcctctttt cacttacaca tactaaaaaa gcaagttgca
     9541 tgccaaaatt agtggcaaca acttgcttat gttcaaaatt cttcttctat cgagagaaac
     9601 tctgtatctt cactcaaaat atcatagata taggcaatct cagaattggg gatcatgaca
     9661 ctataatgct cttcaatgac actaaacgca gatttaatag cagataaccc ttctttttga
     9721 cactgcttcc atttctctaa tccttgatag gtttcgattg gcatttgtcg gatcaatcgt
     9781 tcaatgagac agctcacatg gacatacaac gctaattttt tgggattgct gatgcttcga
     9841 ttcaactgca cctctaattc tctcataaaa tgatcgattt ctcccatcac cttctctgta
     9901 tctagaatag tcacggaatc cagtactttt tccaatgaaa aattacgaat gatgcgctga
     9961 ttgaatattt ccttctctga agcattcata gtagtgtcca accattcagc tagtacagac
    10021 atcttttccc ccgagatgag gtcttctaat gaaagatagt cgatattggt gatatcagga
    10081 tctgtggtgc caatcattcc tacgacttcg tatatggaaa ataatgggtc actgtttttc
    10141 ttatctttta attgctgata ttcatatggg atgattttca actgacaagc cgtaggtaag
    10201 cttttttcta gcagttcact taagtggacc gccgtaccga ttccagtgaa gcaagtagta
    10261 atgagtgctt tgggtttgtt ttcctctgga tagagcagtc gccattctgg ctggatctgt
    10321 cgaacagtct cttctgctat ctcttctaat gatacatttt tttgcaggca ttcccctact
    10381 gtgattgcta aaggtgtcgt cacattattc aaaatcaaaa gcggtacgga taactgtttt
    10441 ttaaaaaact gatggatttc tttcagagat cccatgtcta ctaatatgat caatccattt
    10501 gaaacatcgc tgttttcgca ataatcaagg atttctgaag caatctgctg tggtgtgaca
    10561 tctagtggca tatcaaatga ttcaaataga tcctcttgta ataaacgatt tactacattt
    10621 gccatactgc tagcagttgc ataaccgtga gcagcaatca tcgcttttgg cagtcgtttt
    10681 tcttttgacc atttcgcttt ttttagatag atcgtcagta aaatccgatc catacttgtg
    10741 tcttcaatat ctaatttgat cttacttaat tccagaattc gttggacata gtgataacta
    10801 gatggataag actgagaaac ttgttgttct aattctttga ttagttggat aatatcatgt
    10861 tcttccggta accatttgat agtgcttcgc tgatagagat agtaactcaa ggcatagaca
    10921 ctgtttccat cgaatttgat ttgataggca gtttccattt gtcgaaaggt ctctcggata
    10981 gattgtgtcc aataaaccag cagctcgtgt ttttcttgtc tactggtctc aaacagcaaa
    11041 aaatcaaata attgaatcac ttcttgcttt aattttcctt cgcaagaata tagcagacag
    11101 ttatttttct tgaaatcaat taggatttgc tcaaaacttt taacgattcg ctgctgttcg
    11161 ggctgttgct tctccattaa ttgctctaag gtctggtctt gcgtaatata gattccttgc
    11221 tgtgctgtat ttttagtaat aggctgcgca agtagttctt tttgcaaatg atggatcgtg
    11281 acatggattt caggctgttc acgctgttca gcatatgctt ttgctgctga aactttgacg
    11341 atatttttca gctctcctat gtttccggat aatttctgac ttgttaaaag tgacaaagct
    11401 ggtccactga ctgacaaagg gctccccatt tttctttgtt cttcgagaaa aaaagagtaa
    11461 atcagttcta gccgttcatt acgactacgc tgattaagtg caggtagttc gattcggata
    11521 ggtattcttc gaatgaaagt tgttaagaaa tgactagtaa tctcttcagt cgtagcaaaa
    11581 aagagacgtg ttttgatccg gcgtgacaaa ttaggttctc ccatacgata aatcacacct
    11641 tgatcaagat aggtgaacag tttttcctga ccttctggtg ttaagcgatg tacctcgtct
    11701 aaaaacaaga tcccaccatc tgctgcttca aaagcccctt ttttattttc ttctgcacca
    11761 gtgtacgctc ccttaacatg tccaaacaaa ttgctagtca aaagttcagg attatctgca
    11821 tattgtgcac aattaaaagt gatgaatggt gcagaatctt ctagcagatc atgaagcaga
    11881 caatactgat agaccaaatt aactaaataa cttttacctg tgccactttc gcctgtaatc
    11941 aaaagtggta atccgccatc tggatagtaa agagctgttt tgatttgttc gatactttct
    12001 ttcaaactgg cgtcgtatcc gatcataaaa gagaataagt cttttggttg cgcgaaaaaa
    12061 ggctgttcct ctttgagatc atcgatagaa aaataaaaat tcgtgcgtaa cttaaaaaat
    12121 tgtttttcaa aagcttcttt gtggaaataa atcactggtc gagaattgat tttgactagt
    12181 agttgctcat catttaactg gttcaaataa tgactgactg tgtttctttt taccccaaaa
    12241 tgatcagcta acctttgtgc tgtaaagacc tcgttcatat gttctaaatc gaaaaaggct
    12301 gtctggttct ctaagtaaca taaaatttcc ttcttcaaca acttattcac ccctcctcca
    12361 tcatagaaaa cggattcatt ttgtcaataa aatctttaat aaagaattga aaaatatatc
    12421 ttattataga cagcagccta cttatccctt ataattttta ttctaaaaaa cgtacagttc
    12481 cttccagttt ttatactgga atttcaacat atgaaagaat aaggaggcta tgactattca
    12541 atgaaatcgg ataaatataa aaacaggaaa catacttcct ggaaaacttt tcgagaaatc
    12601 atcggatctt atctcgctgt agttgtattt ttgcctttgc tttatatggc tatgacagaa
    12661 caaactcgtt ttattggcgt agatacgcaa tatatagccg aaacaggatt tttattcatc
    12721 tttatcttga tttttgcgtt ctataaatcc ttccatacaa aataagattc acagatttca
    12781 actttcctgt ctgtttttag ctattacaaa tccttatatg tggtataaca acaatttatt
    12841 ggcataatga ggaaaaacga gttatgatgc tttcagagag taaagaggta ctgttgtcac
    12901 catccttttt attgattacg tacaccactc ttagtttttt cccattgatt acgaaaacaa
    12961 ggtagaaaca ttgatgttcc taccttgttt tcttttttta ttttcagatc aaaatctcaa
    13021 gtggcttttg acttgatgtc gggaggttgt cttcgttcca tcaaacctga tagaatctgc
    13081 gccaaaccat agttttctcc actattattt attcctctaa gatagtaccc acaataaacg
    13141 aataaatatt tttctcattt tcatactcct tctttgaaaa tcaattagat ttctctcaac
    13201 ccactttttt acaaaaatta cgtagcaaaa aagattgcaa gaacaaatta aaaaagaagt
    13261 aaatagagtt tcatctataa acatt
//
LOCUS       DASJTC010000075        13201 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.075, whole genome shotgun sequence.
ACCESSION   DASJTC010000075 DASJTC010000000
VERSION     DASJTC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.075"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..2376
                     /locus_tag="U4B19_002051"
     CDS             4..2376
                     /locus_tag="U4B19_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077779.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="U4B19_002052"
     CDS             2437..3897
                     /locus_tag="U4B19_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEN2077780.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="U4B19_002053"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="U4B19_002053"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEN2077781.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="U4B19_002054"
     CDS             6128..6277
                     /locus_tag="U4B19_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077782.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="U4B19_002055"
     CDS             6352..6945
                     /locus_tag="U4B19_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEN2077783.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="U4B19_002056"
     CDS             6958..7857
                     /locus_tag="U4B19_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEN2077784.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="U4B19_002057"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="U4B19_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEN2077785.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="U4B19_002058"
     CDS             8723..8983
                     /locus_tag="U4B19_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077786.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="U4B19_002059"
     CDS             9428..9634
                     /locus_tag="U4B19_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEN2077787.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9682..9885
                     /locus_tag="U4B19_002060"
     CDS             9682..9885
                     /locus_tag="U4B19_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077788.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="U4B19_002061"
     CDS             9901..10338
                     /locus_tag="U4B19_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077789.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="U4B19_002062"
     CDS             10331..11038
                     /locus_tag="U4B19_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077790.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11418..11597
                     /locus_tag="U4B19_002063"
     CDS             11418..11597
                     /locus_tag="U4B19_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077791.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="U4B19_002064"
     CDS             11922..12185
                     /locus_tag="U4B19_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HEN2077792.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="U4B19_002065"
     CDS             12179..12529
                     /locus_tag="U4B19_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2077793.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="U4B19_002066"
     CDS             complement(12553..13167)
                     /locus_tag="U4B19_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2077794.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         4886 a         2180 c         2716 g         3419 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 a
//
LOCUS       DASJTC010000076        13086 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.076, whole genome shotgun sequence.
ACCESSION   DASJTC010000076 DASJTC010000000
VERSION     DASJTC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.076"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(79..2199)
                     /locus_tag="U4B19_002067"
     CDS             complement(79..2199)
                     /locus_tag="U4B19_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEN2077795.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2429..3607
                     /locus_tag="U4B19_002068"
     CDS             2429..3607
                     /locus_tag="U4B19_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEN2077796.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3623..4381
                     /gene="aroD"
                     /locus_tag="U4B19_002069"
     CDS             3623..4381
                     /gene="aroD"
                     /locus_tag="U4B19_002069"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEN2077797.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4404..4889
                     /gene="ybaK"
                     /locus_tag="U4B19_002070"
     CDS             4404..4889
                     /gene="ybaK"
                     /locus_tag="U4B19_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEN2077798.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4960..6213)
                     /locus_tag="U4B19_002071"
     CDS             complement(4960..6213)
                     /locus_tag="U4B19_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2077799.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6329..7678
                     /gene="gdhA"
                     /locus_tag="U4B19_002072"
     CDS             6329..7678
                     /gene="gdhA"
                     /locus_tag="U4B19_002072"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEN2077800.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7790..9136
                     /locus_tag="U4B19_002073"
     CDS             7790..9136
                     /locus_tag="U4B19_002073"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEN2077801.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9444..9830)
                     /locus_tag="U4B19_002074"
     CDS             complement(9444..9830)
                     /locus_tag="U4B19_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2077802.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10098..10247)
                     /locus_tag="U4B19_002075"
     CDS             complement(10098..10247)
                     /locus_tag="U4B19_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077803.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10709..10921
                     /locus_tag="U4B19_002076"
     CDS             10709..10921
                     /locus_tag="U4B19_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077804.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11199..12998)
                     /locus_tag="U4B19_002077"
     CDS             complement(11199..12998)
                     /locus_tag="U4B19_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEN2077805.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4291 a         2354 c         2577 g         3864 t
ORIGIN      
        1 ttattctaag atttctcttt accaacatca tttgacaaag agcctaattt tgtcgctgct
       61 ccttttaaat ttattttatc attcggaaga acttgtggtc gttccgtgaa gttcttggta
      121 cgtttgcgtt ttataaagat caacagttga ttgtcccttg tgttcactaa agacactagt
      181 tgacttgtca ccaagagatt tttcagcttc ttttaatttc tctaagccat tcttgtagtc
      241 ataatcactg gtatcaattg ggtctaatcc actattcgta tagaaccgaa gaagatcacc
      301 attattgatt tggtcagaaa cagctaactg tttatcaact ttttctttca gtgaatcaat
      361 ttcattctgt acttcctcag taggttcagt aatagcttca ccagtttcgt tactatagat
      421 cgtaccaccg tagtaagtat attttggtgt aacaaaatca ccatcacgga aagaaacaag
      481 ctgttcatga tctttcgaga atagatcttg tcctaactga atataatttt ttgtatctac
      541 acctagtaag tgaagtaatg taggcaaggc atcaacttgt ccgccgtaag tgtgattgat
      601 tccgccctgt gtttgtccag ggatatggat catataaggg acacgctgca tttgtgcatt
      661 gtcgtagttt gtccaagtag aacttgtttt tccaactaat tcagctagat ttttatttct
      721 tgaattcgag acaccgtaat ggtcaccgta aaggacgatg actgaattat catacaagcc
      781 gctttctttc aaataattga agaattcttg tacggcggta tctaaataat tggctgtcgc
      841 aaagtaaccg ttgatcgttt catcatttgt attggctaac gggaatcctg cttcgtcatt
      901 ggtgaatttt gaataaggat aatggttaga tacagcaatg aattttgaat aaaatggttg
      961 ttgcagacgt tccaagtatt gtacggattg ttggaaaaat ggtttatcat gcaacccgta
     1021 ttggaacgaa ttgtcatcat taacatcata ataagaagaa tcaaagaaat aatcatatcc
     1081 taaattcttg tatgtctcgt ttcgattcca gaatgtacca gcgttcccat ggaataccgc
     1141 actagtgtaa ccttgtgttt gacttaaaat atcaggtgca gcttggaaag tattttttcc
     1201 gcctaattgt gtgaacaagg agccttgatt caagccaaac aatgaatttt ccatcagcgt
     1261 ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa aagttgtcaa aactgaacgt
     1321 gctgttgctg tggtaaagac tattgatgaa aggtgtaact tcaagctgga gaccattctc
     1381 atctgtcaat ttataatcga tcaggaattg ctggaagctt tctaaatgga tataaatcac
     1441 attttttcct ttagcgagcc cgaacaaatc atcatttggt gctgcataat gttcttttac
     1501 ataattttct acttctttca tatcattggg acttgcttct gcccgcactt ggtttgtctg
     1561 gtaagtttga acaccgtcat aaaccatgaa agcattcaag ccaagatatt tgaccaaata
     1621 atcacgagag aatgttcgag tcagcaattc tggacgatca gcttccgcca taaaaagatt
     1681 tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa gccattcttg ctcggatcgg
     1741 gcgagggtcc aacttgattt tttttgcaat cagcagaaca gtcaagataa taaaatcgat
     1801 ccaatagatc acatcatatg ggcgaaacag tcgaatggca ctttctccca gaccactcgc
     1861 tactttccca gcacctaaaa tcgtgttgat cgtaatgaag tccgtgaatt ctcgataata
     1921 agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa atgaccatca tcgtgatata
     1981 agcaactcta gggcgtctga tataaagagc aattccaagt aataacaatg tagttgcaat
     2041 agggttgata aacaagataa agtattgaat gccactttcg attccgagat ggaaatcaac
     2101 taaataggca aaaatcgttt tcaaccaaag taaaacaact agtaaagaaa aaaatccgag
     2161 cctcttattc aaaaaggcag gtgtttttat acatttcaag ataatcttcc tttctgcact
     2221 tcttaatcac agaagtgtca tgaaaccatg ttacacagct cacttattct catttatttt
     2281 actagtatga caatatagtg tcaattgaaa ccagaagttt atgctgtttc attaaggttt
     2341 tctttaagct tcatgaaaaa atgagcagta agacaagtcc tatgtttttt gttatacttg
     2401 ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa gtaaaaaaaa gagcaagcaa
     2461 tcgattgaaa aaaggatatc cactgatcca aaaagaagac cttgctcatc cccaaaaaga
     2521 aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa tttatcgcaa aaggatatct
     2581 aggaaagcag aataagggaa tcggttgggt attgagccag cagaacatcc catttgatca
     2641 atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa agaactttct ttttttcaga
     2701 tgattctact accgcttttc gtatattcaa tggagaagga gacgggatcg gcggtctgat
     2761 tgttgattat tatgcaggtt atgctgtttt ttcttggtat aacgaaacgt tatatgcaca
     2821 tcgtgaatta ttgattcaag cttttcgtga aatctacccg gaaatcgtag gaggctatga
     2881 gaaaattcga tttgaatcag taaatctgcc tgagagccag ttcatttacg gaaaagaggc
     2941 accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt gccgtttatt taaatgaagg
     3001 attaatgact gggatctttt tagaccaaaa agaagtacgg ggatcattag cagaaggttt
     3061 cgcttctggg aaaacagttt tgaacatgtt tagttatacc ggcgcctttt ctgttgctgc
     3121 cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta gcaaaacgca gtttaaaaaa
     3181 aacacaggaa cagtttgaag tgaatggttt ggatgtcgat cagcaaaaaa tcattgttat
     3241 ggacgtattc gagtatttca aatacgccaa aagaaaacaa tttttatatg acatgattgt
     3301 attggatcca ccaagttttg ccagaaataa gaaaaaagtc tttcgcgtag caaaagatta
     3361 tggggaactg gtcaaggata gtctggatat tttagcagaa gacgggatac ttattgcgtc
     3421 tacaaatgct gcgaatgtct cgatagaaca attccaaaca atgattgaag aggaattcca
     3481 tgccaaaaat gtttcttttg aacaacttgc tttatatcgt ctgccaaaag atttccagac
     3541 gacggatacc tttttagaag gcaattatct gaaagtgttt gtttatcaaa ttaaaaataa
     3601 ccattagaag gagcgatgca gaatggtttg ggtaaaggga ttgaatatag gaagcggtag
     3661 accaaagatt tgtgcgcctc ttatcggggc cgtccgagag gaagtattac gtgaagcagt
     3721 tcttgcacgt gaagcagggg ccgatttagt cgaatggcga gtcgatcatt atcgtgaagt
     3781 tttttctttt gatgaagtag cagaaacatt ggttcttatc catgagatgc ttgatggact
     3841 gccgttatta tttacgtttc gtaccttagc tgaaggcgga gaacaagaca tatcgatccg
     3901 tcagtatcgc gacctttatg aacatgtgat acacacagga ttagtagata tggtcgatat
     3961 cgagttgttc aaagtagaaa gtctaggaaa aagactgata gaggaaatca agtctttaaa
     4021 cattccttta attatcagta gtcatgattt taaagaaaca ccagcagacc ctgtcttgct
     4081 ttaccgattg aatgttatgg aacattttgg agccgatatc ggaaaactgg cagtaactcc
     4141 caataatgaa cgagatgttt tgcggttgat ggagttgact agacgggcaa atgcttttgt
     4201 ctccatgcct attattacga tgtccatggg aaatcttggt aaaatttctc gtctggctgg
     4261 cggaacgacg ggttccgtta tgacatttgg ttccttaagc agcgattcta cttctgctcc
     4321 aggacaatta ccggtcaaag cattgaagca aattattcaa ttattaaatg aaccgttgta
     4381 gaacaagttt gaggtgatat tttttggcaa agaaaaaaat agtaaaaacg aatgcaatcc
     4441 gtatggtcga acaaaaaaag attccctata cagagcacga atatgagtgg gacgaaagcc
     4501 acctaagtgc gtcatcggtc gctgaacagc ttccagaaag ccaatctcat atatttaaaa
     4561 cactggttgc tgtgggcaat gtgacggggc ctcttgttgc tgtgataccg ggagaagccg
     4621 aattgaatct aaaaaaatta gcaaaagttt cagggaataa aaaagtagaa atgctccatt
     4681 taaaagattt ggaagcaacg accggataca tacgtggtgg ctgctcgcct ataggtatga
     4741 aaaaactatt tcctacttat ttagatcaaa ttgcagaaag ctatgaacaa atcattgtct
     4801 cggctggcag aagagggcta cagatggagt tagcacctca agatatctgt gcattaacga
     4861 gcggacaatt tgcagatata aaacaataaa tcatacgtat aaaagaaaga aggaggccgg
     4921 gatgttgttt gtccagtctc cttctttctt ttttatacat tatgctcttt gcccggcatt
     4981 cattcctgct acatgccctg tagcgaaagc cgctgtgata ttaaagccgc cagtataacc
     5041 attgacatcc agtaattctc ccgcaaaata cagaccatca atgattttac tctccaatgt
     5101 tttaggataa atttccttta gagaaacccc tccgccagtt acaaatgatt tctcaatagg
     5161 aaaagttttg ttgattgata gcttaaactc tttgcatagt tgaacaaaat cttgaatttg
     5221 tttttgagag gcttgttgtc ctgtcagatg atccatttca agccgctcaa gaaagaaaac
     5281 taacaatcgc tcaggcaata taccatgcca agcattgacc aagttctttt tggttgcttt
     5341 acttttttct gtcaatatat gaataagctc ttgtttggtt tgtgtaggga aacaatccag
     5401 agaaagtgtc actggttctc ccgttttctc taattcttta ttgacaaaac ttgaacaacg
     5461 cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt aaatccatcg tatgttcagt
     5521 caatacacga ccttttttat ttaaaacgcg taaagtgatg tcttgtaaag aaagcccttg
     5581 aagtgttttc tcctgaatat acggttcatc cgatatcaag ggggactctg tcgcataaag
     5641 aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac ccatctccag tagaaccagt
     5701 agatggataa gtccgtccac cagttgttaa gataatggca ggagcatgga actcttcgaa
     5761 ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc aatttctcta ctcgagtaga
     5821 gaatagaagc gtcacatcta gttctttcag acgattgagc aatgcttcga taatcgtttt
     5881 tgatttattt gttacaggaa acatccgtcc atgatcctct tcttttaaat gtacgccttg
     5941 tgattcaaag aagttcatga tatcgaaatt attccattgc gcaaacgtac tatataaaaa
     6001 ctttccattt ccaggaatat gtgcaatcaa atcatcaaca ggacgattgt tcgtaacatt
     6061 gcaccgacca ccacctgtca tcagtagttt ttttcctgct tttttgtttt tttcgatcaa
     6121 gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc atcatcccgc ttgttccagc
     6181 gccgactacg atcacatcat acagtttttc cattccctca cctcaaggaa agtgtagcat
     6241 atctgaagaa agaaaacaga aaaaatgttt tgagccaaaa gagaaaatga gctaaaatga
     6301 gggaagataa gcaaaggagc gtggaacgat gacaaacgca acagaatatg ttcaagcaat
     6361 tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa tttttacaag caatcgatga
     6421 attcatgccc acggttatgt cttttttgga cactcatccc gaatatatcg aaaagaatat
     6481 cttagaactt ttaacggaac cagaaagagt tattcaattt cgagtgcctt ggcaagatga
     6541 tgaaggaaac tggcgagtaa atagagggta ccgtatccaa tacaactcag ctatcggccc
     6601 ttataaaggt ggattgcgct tccatcctag tgtcaatcta agtatcttga aatttttggc
     6661 ttttgaacag attttcaaga atagtttaac cggtctgcca atcggtggag gaaaaggcgg
     6721 aagtgacttc gatcctaaag gaaaatctga taatgagatc atgcgttttt gtcaaagttt
     6781 catgctggaa ttgtcaaaac atatcggccc atcgattgat gttcctgcgg gagatatcgg
     6841 tgtaggagct agagaaatcg gctatttgtt cggcgaatat aaacgattga aaaaatatga
     6901 tactggtgtg ttgacaggta aacctttgga tttttgggga agtaagatac gtacggaagc
     6961 tactggttat ggattagtgt attatgtcaa acatctattg aacgaagaaa aagattcatt
     7021 taatcaaaaa acagtttttg tttccggaag cgggaatgta gccatttatg caatcgaaaa
     7081 agtgcaagag ctaggcggga aagttgttac atgttcagat tctagcgggt acatctatga
     7141 tccagaagga atagatgtga aactattgaa agaagtcaaa gaaatcaata gacaacgttt
     7201 gacggaatac gcaaaccaac gtccttctgc tatttatcat gctggagaat ctgtttggac
     7261 attgaaagaa aaagcggata tcgctttgcc atgtgctact caaaatgaaa tagacgaaag
     7321 cttggccgaa atcttagtag aaaatgggat caaaatcgta gcagaaggcg ctaatatgcc
     7381 atgtacagta aaagctgttt ctgttcttca tgaagcaggc gtgtggtatt gtccaggaaa
     7441 agctgctaat gctggaggag tagcagtatc tgctttagaa atgagtcaga acgcccagcg
     7501 attgacttgg gaaagagagc aagtggatga gcagctggat caaatcatgg aaacgattta
     7561 ccatacgtgt cgccaaacag cagccgagta cggcgacgaa aaaaatctgc ttcttggagc
     7621 aaatgttgct ggatttgaaa aagttgccaa agcaatggca gcagaaggtt tagtttgaaa
     7681 aatgaaactt tcttggaaat attgtcagca ttttgtttat ttgcttgaaa aataataaaa
     7741 aaaaacgtaa agtattacct gtagagtaaa cctataagga ggagaacaaa tggcacacat
     7801 tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa caagagttag ggtacatgca
     7861 aagccaagta acagcagccc acaacgaatt acgggaaggt actggcgcag gaagcgactt
     7921 ccgcggctgg atcgatttac caacgaacta tgacaaagaa gaatttgcac gtatcaaaga
     7981 agcagctaaa aaaatccaat ctgattctga agtattagtt gttatcggta tcggtggttc
     8041 ttatttaggc gctcgtgcag cgatcgactt cttgaatcac acattcttca atttattaga
     8101 tagcaacaca agaaaagcac cacaaatctt ctttgccgga aattcaatca gctcaacata
     8161 tattgctgac ttgatcgaag taatcggtga tcgcgacttt tcagtaaacg ttatttcaaa
     8221 atctggaaca actactgaac cagcgattgc gttccgtgta ttcaaagagc ttcttgttaa
     8281 gaaatatgga caagaagaag caaacaaacg tatctacgct acaacagata aagcaaaagg
     8341 tgcagttaaa gtagaagcag atgcagaagg ttgggaaact ttcgtgattc ctgatgacat
     8401 cggcggacgt ttctctgtat taactgctgt tggtttgtta ccaatcgctg ttagtggtgc
     8461 agatatcgat gcattgatgc aaggtgccgc tgatgcaagc aaagcatact caagcgacaa
     8521 actagaagaa aacgaagctt accaatacgc agcaatgcgc aacattcttt accgtaaagg
     8581 caaagtaact gagttgttga tcaactatga accaggaatg caatacttct cagaatggtg
     8641 gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa ggcatctatc cttcaagcgc
     8701 aaacttctca actgatttgc actctttagg acaattcatc caagaaggac gtcgcaatat
     8761 ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca atcacaattc ctgaacaagc
     8821 agaagattta gacggtctag gctacttgca aggaaaagaa gtggacttcg taaatacaaa
     8881 agcatttgaa ggaacattgc ttgctcatac agacggtgac gtgcctaact tgctagtcaa
     8941 aattccagaa atggatgctt atacattggg ttatacaatg tacttctttg aaattgctgt
     9001 aggtatttca ggttacttga atggaatcaa cccattcgac caaccaggtg tagaagctta
     9061 caagaaaaac atgttcgcac ttctaggcaa accgggcttt gaagatcttg ctaaagaatt
     9121 gaacgaacgc ctgtaagata agggtttttc gctagattta aataatcagt gatagaaaca
     9181 tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact caaacatact gcatcgttta
     9241 ttcagaataa ggggctgtga caagacgttt gtcacagtcc cttgtttgta tagaaatcga
     9301 ttgattgcct tgctctttag tttgttttag gactttaaga acagtgaggc tgacttcgaa
     9361 ggttaattct gtttttttgt tttctagaat cagtaagcaa aaaagcactc acttcgagtg
     9421 cttttttgcg atatattctt agcttaagat aggcctaatt tttcttttac agatgagggt
     9481 accttatcgc tggtaattac ttttttctct atgatccgct tttcgcttgc tttcaactgc
     9541 aagatcgttc cttcggtgta agcagaacca ttggcagagt caccagaagt aatgacatcg
     9601 aaatctaact gtcttttttc accgttttca gcataagcat ctacgttgta tttaaaacct
     9661 cttccaatta cttccccatt atctgctttg atatcagttt ctgcaggcat tggagctttg
     9721 actaccgcat aataatcttt acctacataa gtagactgat agtagtccca acctttccat
     9781 cctataaaag caatcaatat gataacgatc aaccctaaaa tttttttcat gaaagatctc
     9841 cttttttatt tttattatat taaatattat acagataata acggaatatt atcatcgaaa
     9901 cagctaacaa aattgtcata aaaaacgaga tggttgatat tagaacttgt gtttttgttt
     9961 aggtctagag ataaagagag acaatttcca aatatatgta atgaatacaa aaagacgttt
    10021 gtcgcggatt accgttacaa acgtctttta ttgattttcc gagatctcat cagtcgccat
    10081 ttggaatggt tcactcctta tctagattcg tttaatgctc ggactgcttg ttcgattgta
    10141 ataccaaacg caacgttatt ttcgttttgt gcatcaatcg ccataaaacg gttagaagtt
    10201 gtatcaaaag ctacgcggta ttgcatattt gtttttgtaa acgtcataaa attcatctcc
    10261 tttgtaatgt aatttagttc ttgaaagttt cctttaaact ttcgaactta taccaagttt
    10321 agcatcgtac gttttttgat gcaattaaaa agcatttatc attttcctta tttatttttt
    10381 cttaaatggt ttagctattt tcttttaatc cgataagtag cccaatcatc aaggttacaa
    10441 atactattgc ttgaatataa atcatatgct cacctcttta taaataactt agcaatataa
    10501 agaaaaaaac gcacgaacaa cataggaata taaacaaacg tttttgtcac aacgtctaag
    10561 atcgtgttaa aaaatagtta tctaaaaagc agaaagatac tcaaaataaa aaataaaaaa
    10621 atgaataaaa acgttttcaa atacaaggta atttgttatt ataaaataaa gaaaaaacaa
    10681 aataaaaata aagaaaagag ggaatgaaat ggtaaaagca gaagtaggaa gatcttatat
    10741 atgcaaaccg atcggagtat ctcatgaact aatcggctgt attgaacgta catatatcaa
    10801 cacggctgtc gtgtatgttg aaagttatga ccaacgagac tatcaactta tcaatgaaag
    10861 caagtatcgc atgctcgtaa aattctgtga tattagcgca cctactgaat tggtttctta
    10921 aacagtattt ttgagaattc gcaagaactc ccttataata taaataaaga cagaagggag
    10981 cgagaagata tgccaaaaag aaaaagttca gggaaaatgg gaattattct tgcagtgatc
    11041 tctgcaattc ctgctatttt agctattgtt cgtgcgatga aggaatcaag agaaagttaa
    11101 gcacacatta tgagcgtcaa acaaaaaacg catatacctg cggtggcagg ttatgcgttt
    11161 tttgttgtat aaagactttt ttcattaacg atttttgatt agccgactcc caaaacaaaa
    11221 tttagcagtt tatctgaata agtgatttct tcatctgttt gtttcacact ttcatttagt
    11281 agtcccatct catcggttat gataccatcc acaccataaa acatcatgcg actcatggca
    11341 tctgaatcgt tgattgtcca tacatatact tcttttccgt cttggtgggc agaattaatg
    11401 aagttacggt tgatcgtact atattccatt gtcagaaaat cagcaggcgt cacagggggc
    11461 ccaacaatat tgaaaggtaa gatgtagccc acataaaagt ttggttctgc ttcttttaaa
    11521 tctgaaacgg tttggtacgt caaactttgc aaaatatgat ggttctctaa gataactttg
    11581 cggtatttat ggacaaatcg ttcgacaagg tcatccgaat cttttttaga ggtcttgatc
    11641 tcaataagta atttttggtt tagcttatgt gctttttcca aataagaatc aaaagaacaa
    11701 acaggggctt gcactccgtt ttcagtaact gtaagatttg tcaattcttt caatgtaaga
    11761 tcctgtggtg ttccatttac ccctgttaag tgacgaagat ttgcatcatg catcactacg
    11821 aaatgaccat cttttgtttc ctgaacatcc atctctacat aatcaggatg atagtctctg
    11881 cttgtttttt caagtgcttc taaagtattt tgcgccccat tttgggtact cacacctctg
    11941 tgagagacag tcactggttt tgtctgaaca gctgaagtca gataatccat attgtaaaga
    12001 cagacgccaa taccgaaaaa aactgcaaaa agagtcaaac cagtgttttt cagcgcagag
    12061 aaacgcaagt tgggggcttg tgggataaac cacttaggga tctcaggcaa gaagccttcg
    12121 tcatccatga aatcaacaat gatataaaaa atccctacgg tagacatcac gatattgaat
    12181 aataaaatcc cttgtaaaag cgtcatagca aacacagcgc tgatcaatgc atagtctggg
    12241 aaaaattgtt caaccatact ttgtgccaaa ataacaacga tatatcctaa agaagaaagg
    12301 agtaaaatcg taccgccaat cacaataaat tggcccgtga tggccaaaag acgcgatttg
    12361 gtcaatgacc agctttcccg aatggctgct cgaaaagaac gatctcgtag aatcatttcc
    12421 ggtaaagcaa aaatcagtcg aatcccaata tagccaagga aaatataaac gagtaaaaag
    12481 gaactgacga tgatccaacg attagcaaaa ataaaatcca taatgaaagc ggggattttt
    12541 atttttgaca gtagatcaga attaaaactt aatccgctga tcggtagaat caataaaaaa
    12601 taagcaagga aaaacaaaaa agtaatgggt ctcactttct tcagctgaag tatcgtcaag
    12661 tgtaagagtt gcttcaaaga gattggttct ttctttttaa taaaaaaaac actcatcaaa
    12721 agaaaagtaa attcgaaata aactaacagt aaaatcgtca tcaaaactac gattaacgag
    12781 agtagaaccc caggatgttg agaaaaaatt gaaggaatat tgtcataaga caaatagtca
    12841 attgcccctc ggtgtaaaat aaaacgggtc gtactgctca ataagggtaa acaaataaat
    12901 aaaataaagc catgcatcag caagacatca cggaaataag cttgtgttcc ttttaaaaag
    12961 tcccaagcat tacctaagct acgtttcaaa taattcatcc tatcacctgc tcttcacatt
    13021 gtagcgcaaa tctaatgaaa aaggaaaggg tgcagtgtgt tttgcagacc ctttcccaga
    13081 tttaac
//
LOCUS       DASJTC010000077        13051 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.077, whole genome shotgun sequence.
ACCESSION   DASJTC010000077 DASJTC010000000
VERSION     DASJTC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.077"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            152..1075
                     /locus_tag="U4B19_002078"
     CDS             152..1075
                     /locus_tag="U4B19_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN2077806.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            1081..2334
                     /locus_tag="U4B19_002079"
     CDS             1081..2334
                     /locus_tag="U4B19_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEN2077807.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            2468..3814
                     /gene="gor"
                     /locus_tag="U4B19_002080"
     CDS             2468..3814
                     /gene="gor"
                     /locus_tag="U4B19_002080"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEN2077808.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(4628..5581)
                     /gene="menA"
                     /locus_tag="U4B19_002081"
     CDS             complement(4628..5581)
                     /gene="menA"
                     /locus_tag="U4B19_002081"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEN2077809.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(5594..6697)
                     /locus_tag="U4B19_002082"
     CDS             complement(5594..6697)
                     /locus_tag="U4B19_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEN2077810.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(6787..7743)
                     /locus_tag="U4B19_002083"
     CDS             complement(6787..7743)
                     /locus_tag="U4B19_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEN2077811.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            8024..9958
                     /locus_tag="U4B19_002084"
     CDS             8024..9958
                     /locus_tag="U4B19_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2077812.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            10415..10831
                     /locus_tag="U4B19_002085"
     CDS             10415..10831
                     /locus_tag="U4B19_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEN2077813.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            10845..11411
                     /locus_tag="U4B19_002086"
     CDS             10845..11411
                     /locus_tag="U4B19_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEN2077814.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            11434..12420
                     /locus_tag="U4B19_002087"
     CDS             11434..12420
                     /locus_tag="U4B19_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEN2077815.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(12575..>13051)
                     /gene="scm"
                     /locus_tag="U4B19_002088"
     CDS             complement(12575..>13051)
                     /gene="scm"
                     /locus_tag="U4B19_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEN2077816.1"
                     /translation="STDTSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSST
                     TTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNR
                     TNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         4065 a         2369 c         2695 g         3922 t
ORIGIN      
        1 tcacaatctc tttttatatt tggacagaat atatcgaact ttattttgat tttttgtgaa
       61 ataattttca aaaatccgac tttaaataga agaagtattg gaaagttttt agagctatgc
      121 tactattatg aagttgtagg aggagaaaat tatgatcgaa ctcataacca cagtagaaac
      181 tattgaacaa gcagaacagt tgcttgctgc aggtgcagat acactatatt tcggtgaaga
      241 gcaatttgga ttgcgtctgc ctgcttcatt tacacgagaa gaacagcgac aattggtaga
      301 attagcacac aagtatggta aaaaagcgaa tattgctgta aatgggatca ttcatcctga
      361 aaaaatgaaa aatgtacctg aatatttggc ttttttaaaa gaaataaacg tagatgtgat
      421 cacagttggt gatacaggta ttatttatac gatgaaaaaa aatcctgaat tgcgtatacc
      481 gtatatttat gatgctgaaa cattagtaac gagcgcacgt caaatcaatt tctgggcgaa
      541 aaaaggcgca gttggagcag tacttgctcg tgaagttcct tttgaagaga tgaaggcgat
      601 ggaagaaaaa ctcgatattc ctgtagaaac ccttgtgtat ggtgctacat gcatccatca
      661 atccaaacgt ccactgctac agaattacta taactatacg aaacaagacg aacaaaaaga
      721 tcgtgaaaga ggtcttttta tctcggaacc taaaaaagaa gagacacatt attctattta
      781 tgaagatagt catggcacac atatttttgc cagcaatgat ctgaatctct cgaatgaatt
      841 gaatgaactt tatcagcatc attactgtac atggaaattg gacggattat ttactccagg
      901 cgaaaaattc gtaaacatca ctcgactatt cgccgaagca aaaaaacaaa ttttagaagg
      961 tagctgggat caagaaaaag cggaaaaaat gactagcgaa atcagtaaat tgcatcctga
     1021 aaaccgtgaa ttagatacag gattctttta tattgatcct tctgctatca aataaggagt
     1081 atgatgaaag aaatgacagt aaaaagaaaa ttgaaacgac cagaaatcct agcgccagca
     1141 ggaacactgg aaaagttaaa aaccgcgatc cattatggag cagatgcagt atatattggc
     1201 gggaatgcat atggtttaag aagccgtgca ggtaatttta cttatgaaga aatggctgaa
     1261 ggagttgctt ttgcaaaaga acatgatgcg aaggtatacg ttgctgctaa tatggtcacc
     1321 catgaaggaa atgaagatgg agcaggggaa tttttccgca gtttgcgtga tgtcggtatt
     1381 tctgcagtga ttgtttctga tccagctttg atcgaaattt gtgcgacaga agccccaggt
     1441 ctgccgatcc atttatccac tcaagcttca gcaactaatt ttgagacatt ggaattctgg
     1501 aaagaagaag ggttggaacg tgtcgtttta gctcgtgaag tttcaatgga ggaagtggca
     1561 gagattcgta aaaatacgga taccgaaatc gaagcattta tccatggtgc catgtgtatc
     1621 tcttattctg gtcgatgcac attgtccaac catatgtcga tgcgggatgc aaaccgtggc
     1681 ggatgttctc aatcttgtcg ctggaagtat ggattgttcg atatgccttt tggtcaagaa
     1741 cgaaacagtt taactgatac aggcgaagtg gaagaagaat tctcgatgag tgcagtggat
     1801 atgtcaatga tcgaacatat tccagaattg attgaaaacg gcgtagacag cttcaagatc
     1861 gaaggaagaa tgaaatcgat ccattatgta tctacggtgt caaatgtcta taaagcggct
     1921 gtagacagtt atatggaaga tcctgaaaac tatgtctgca agcaagaatg gatcgacgag
     1981 ttatggaaag tagcacaacg agaattagct acaggttttt actacaatac accaactgaa
     2041 aacgaacaat tattcggtga acgtcgtaaa atcccagtat ataaatttgt cggtgaagta
     2101 ttatcttatg atgaggcaac acagattgct acgatacgtc agagaaactt gttttctgta
     2161 ggagatgaaa tcgaatttta tggtccagga ttcagtcatt tcagtcaaac cgttgaagtt
     2221 atgcataatg aagaaggcga atccattgac cgagcaccaa atccaatgat gattttaact
     2281 atgccagtcg cacagccggt gaaacctgga gatatgatcc gaaaacgtaa ataaggaaaa
     2341 agctgatata tccctgtttt aagcatcttc gcatacaaaa ttcaagaaaa gtgttacact
     2401 tgttttctgt agaaaaagga tcgttaaata aaggttttga ttgatctctt caataaggag
     2461 gaacaaaatg caaatatatg attacatcgt aattggtggc ggctctggcg gtattgcttc
     2521 agcaaacaga gcagggatgc atggcgcaaa agttttgctg attgaagcga atgaattggg
     2581 cggaacatgt gtcaacgtcg gttgtgtacc taaaaaagtt atgtggcaag caagtacgat
     2641 tttggaaaca attgaacgag atgcctcaag ttacggtatc caagcagatt taaaaaatgt
     2701 tgatttcgcc ggtctagtag aaagacgaga aaaatatatt gattttctac acggtgctta
     2761 tcaaagagga ttagatagca atcatgtcga ggctataaaa ggttacgcta cttttatcga
     2821 tagtcaaacg atcgaagtaa acggtgaaac ctatcgtgct cctaaaatct tgatcgctac
     2881 tggaggccgt gcaagtaaaa tgaccattcc tggtggagaa tacgcaattg attcaaatgg
     2941 attttttgaa ttaacagagt tacctaagag agcaatcgtt ttaggtgctg gatacattgc
     3001 ggctgaactt tcaggtgtat ttcgtggttt aggtagtgaa gtcatgtggg catatcgaaa
     3061 agaacgacca cttagaacgt ttgataaaat gctttcggac aatttgatcc aaatgtacca
     3121 agaagctgga ataaaaactt acgcacatca tattgctaaa gaaattacta aaaacaatga
     3181 cgaatatacg gtgctatttg aaaatggtga aaccttaact ggtgattgtg tactttttgc
     3241 cggtggacgt gtaccgaata ccgaaaaatt aggtctggaa aatacaaatg tggaattgga
     3301 caaaaaaggg tttatcaaag tagataaata ccaaaataca acggatgaac atatttttgc
     3361 tgttggtgat gtgatcggta aattagatct gactcctgtt gcaatcgcag ctggtagacg
     3421 tttatcagaa cgtttattta atggaaagaa agattcttat cttgactata aacttgttcc
     3481 gacagtcgtc tttacgcatc caccaattgc aacaattggt ttgacagaag aagaagcatt
     3541 ggaaaaatat ggcgaaaatg aattgaaagt ttatcgttca cgctttaccc caatgtactt
     3601 tgcattaaat gattatcgcc aaaaatgcga aatgaaattg atctgtgtcg gaaaagaaga
     3661 aagaatcgtc ggcttacatg ctattggtat tggtgtcgat gagatgctgc aaggttttgc
     3721 cgtggctatc aagatgggtg caactaaaga agactttgac aatacagtag ccatccatcc
     3781 aacaggagcg gaagaattcg taacgatgcg ataatttaat attaacagtt ccaacagctt
     3841 tttattgaac aaaccatgta aaaccagaaa atcagtatac tgattttctg gttttttgtt
     3901 ttttagttat ttatgtaaga ttgcttcgat aattctcaaa acgaaataag tagggaagtt
     3961 ggagattttg attatcttcg taactgacga agagtaaatg aatccatttg gtatgcactc
     4021 ttaagttcaa aatattggta tcgatgttta aaagaaatag aaatgtatcg aagaacagat
     4081 gtttaaaaaa gtgtgacttt gtataaataa attagaagaa gcaaacgagg atgttaaaag
     4141 aataccatat agttaccaaa ccaagaaagt tctcgtcaag ttcgctggta cgaatcaaat
     4201 caagtgaaaa aagggactgt gaaaaaaatt gtgtcatagt tagtcacttt ttccgaacaa
     4261 atagtgttca atcagctgtc cttcggtatt agctcaaaaa acggcaaaat atgaggaact
     4321 atttttccat ttgtttctct aatactcagg acaaaacact gattttacaa cctctgataa
     4381 acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa tgtggaaagc
     4441 cattttcaca aaatttttct taatactcga aactaagcga ctttttcaca acctcctgat
     4501 aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa aaatataagg
     4561 agctattttt atttggctgt tcttattact tgatgcaggg cagttttttc acaacctctt
     4621 catgctgtta aatggcttgc ataatgattg ttagtaatag ccccaataca taactggcat
     4681 tgaataagac catatttttg atggaatagc tgaaactgat aggctggggt aacgtttgct
     4741 tgaattgatc caaatgcttc ttcacaattg gcaaggtcaa gaaggtgacc aagatcggcc
     4801 attcatagat accgctcaac aaccccatta aaatcacgaa atagcaagca tacatcaata
     4861 gctggaataa gactgttccc gcaggacgtc cgatataaaa aactagtgta tagcgatgat
     4921 tttcaatatc acggtcaaga tcccgcaaat tattagccaa catgatattg gcaatcgtaa
     4981 acaccaaggg taaggaagct aatacgattg ctgctatagc ccaaagattt cctaccaaag
     5041 caaattgtcc agatgaaaag ttgactgtca gatcatagac atctgaaccg aatgtattga
     5101 cataaacgac catggcaaaa attccaaggc ccatagtcac cccactgaaa atttccccta
     5161 aaggcattcg agataaagga atcggtccga atgtatagaa tactccaata aaacaacaga
     5221 taaatcccat caaaagtaaa agccaacccg tattgatcgt tagaataacg ccgataacaa
     5281 aaacaaatgc aatcatagct aaaatcatgt ttcgaatcaa tacaggagat agatttctgc
     5341 ggccaatgac attttcttca tacttatata cttcagaatg agccttttca taatccatgt
     5401 aattattgat agcggtagtc gctaaatcaa aaactagcat tccaataaaa aataaaaaag
     5461 tgtttagcca atgtatctca tgaaaaaaag cgatagagaa taatacccct accgcaaaag
     5521 gaaatacact agccaccttt gttcgtattt caactacttc taaaaagatt tttagtgtca
     5581 tggttcgtac tccttatttt agttcagaac ggtcacccat cgtgtaatgg gagtcctccg
     5641 ataatttgaa ggtgtctttg attggatcgg tcacatatac tttatcgtct tttgtcacga
     5701 agatcgcttc cgtcccgtta ttcaattcgc tttcgacgta ttccagtcct tttttcacac
     5761 ccattgaaaa gactgctgta gacaacccgt caccatcaat tgatttatct gttatgatcg
     5821 tgacacttgc gatatcattg tcgaacggat aacccgtttt tggatcaaac aaatgatggt
     5881 attttttgcc atctacttct aaataacgct cgtaaattcc agaggtaact aatgttttgt
     5941 tccgttcttt aatagaacct aaaacagatc cgcgagccat gttcggatct tggatgccga
     6001 ctgtccagtc ttggttttcg cctcttgggc tgtggcctaa gacatagaca ttaccgccca
     6061 agtcaacgat ggcagtggtc actccttgtt ttttcaagac tttcactact tcgtccgtaa
     6121 tgtaaccttt ggcgattgca cctaaatcga tgatcattcc tttttctttt agataaacag
     6181 ttttttcttt gtcatttagt tcgatcttat gataatccac taatttcaat gcctgatcga
     6241 tttcttcttg ggaaggtttt ctggcgtcat caaaaccgat tcgccataat tgtgtgattg
     6301 caccaatcgc catatcgaat ccgccttgag aatcttggct atattcgtag gcccgcttga
     6361 ctaacgtgta gacgtcatca gaaactttta ctggtttgat cccagcttgc tcattcactt
     6421 catcgatctc agaacctttt tgattgatcg tgattttatc accaagttct tttactcgat
     6481 cgaaggcagg tttcaatgct gattctttgc cttcatcata cacacggata cgaacgtaag
     6541 tccctaacaa gaactgttca tcagaatatg gatctttcaa tatcttggta gctgctgaac
     6601 tttcagtggt tgttttattt gctgagttgc tgcagccagt tagcaccata aaagagatgc
     6661 ccagtgtgat taaggaaaat agtagttttt tccgcatctt tttcctctct tcattttgaa
     6721 aggtgagacg actgcctaat ctggctatcg tcccaccctc caacttttat tagtatgatt
     6781 atgtgattat ttttctgtta cgatattatc tacttcgatt gtttgagtgt cgcctttttc
     6841 agctgcattc actaattgtt gtgcgtacat gatgaatgaa tgtgagctgt gtgtagcacc
     6901 tgtaacaact tctacattag caggtgagcc atcttcttcg cccattgctt tgacgaattc
     6961 gtcgttcaga gtagggatgt aagtttttgg ttctgtaccg ctttttgctt tcatattttc
     7021 attatattct gtatcatctt gttttgattt accgtctttg tcgatataat caaagtttga
     7081 ttcagttact ttgccgccag caactgtcat ttcgaattgc acacggtagc cgttagagta
     7141 gttcttttct tcaagcttgt aagtgccgtc tttcaaatca gcaccattgt cgatttcgat
     7201 tgtatctgtg ttaccagctt gagcagcttg gatcaattgt tgtgcgtagt tttggaagct
     7261 ttcagatgag tgagtagctc cagttacgac ttccacaccg cttgcgcttt gtgcttttaa
     7321 gaattggtca ttcaactcag gaatgtattt ttctgggttt gtgccagatt ttgcttccat
     7381 gtttttgttg tattctgcat cttttgtttt tgattcgccg ttttcattga cgttatcata
     7441 ttttgattca gtgattttgc cgtcttttac aaccatttca aagactgcac ggtaaccatt
     7501 tgaataattt ttttcttcta atttatatgt gccgtcttgc aaatctccgc cagctacaac
     7561 ttttgcagat gattctgttg tgctttttgc agtcgaagat tgtgctgtag atgattcaga
     7621 agaagagttt gagcttccgt tgtcatctgc gccgcaagcg cctaacacta gagctgaaaa
     7681 tgctaatacg gcaaatccag ttacaaaacg ttttttcttc attttaatac cccaccattc
     7741 catttctagt ccacaatttt tctttgtgat tcattataca ttctaatctt tttagcctat
     7801 aagtcaacta tttttgataa ataacccatg taatatttca aacgttttct ttcagcaaat
     7861 ttcgtgaaaa aacgctttat tttaaaattt gaaaaaaatc caaaaggagt tagacaaaag
     7921 aacagaacat ctatataata tatggagatt gtgtaataag tatcaaacta aaagaattat
     7981 catatattca ttatatagat agtctataag gagcggatga tcaatggcaa agcaaaatat
     8041 tgtcgttgta ggagcaggat atgccggtgt ttcagctaca aaatacttgg caaagaaact
     8101 gaaaaaagaa gatgtagcaa ttactctgat cgatcgtcat tcgtaccata ctatgatgac
     8161 cgagttgcat gaagtagctg gcggacgtgt agaaccagaa gcaatccaat acgatctgca
     8221 gcatttgttt gctcgtcaaa agaatgtaca acttgtcaca gacactgtaa tcggtatcga
     8281 taaagaacga aaaatcgtca agaccaaatt aggttcctat ccttttgatc agctgatcct
     8341 tggaatgggc ggagagccga atgactttgg tacacctggt gtcaaagcac acggattcac
     8401 cctttggtca tttgaagatt ctgtaaggat tcgggaacat attgagaaga cagtagcaaa
     8461 agctgcattg gaaccagatg atgaactgcg taaagccatg ctgactttcg ttgtctgtgg
     8521 ttctggattt accggtattg aaatgattgg ggaactgatc gactggaaaa atcgcctagc
     8581 caaagattat aaattagatc caaacgaaat cacactgatg gtcgtagagg caatgccgac
     8641 gatcttgaat atgttggatc gaaatgatgc tgcaaaagct gaacgctatt tgcgtaagca
     8701 caacgtggaa ttattattga atgcaccaat cgtagaagtg gctgctgatc atatcaaatt
     8761 gaaagacgga cgcaacattc cgactcacac actgatttgg acagctggtg tcaaaggaac
     8821 ttctgacgct gctgactttg gattagaatc agcacgcgga cagcggttga tcgccaatga
     8881 atatatgcag gcaaaaggct acgaagataa aaacatctat atcattggtg atttagtctt
     8941 ttatgaagaa ttcccaggaa caccgacacc gcagatcgtt caagctgctg aacaaacagg
     9001 acacacagcc gcagcgaata tcgtggcttc gatcaaaggc ggagaaaagc ataaatttaa
     9061 aggaaactat caaggattta tggtttctat tggtgccaaa tggggggtag ctaacctatt
     9121 tgataagatc catttaagcg gatttttagc tatcgtaatg aaacatatcg tgaatttgaa
     9181 atacttcttt gatattcgtt ctggttatta tatgttccaa tatttaatgc atgagttttt
     9241 ccatatcaaa gatgatcgca acgtgactcg aggacattct tctcgttacg gcaatgtctt
     9301 atggagtgtg ccacttcgta ttttctatgg tttagtttgg ttcgttgaag cttccaaaaa
     9361 aatcattggg aatggtgaag tgttcaaacc aagtacttgg tttggtgaag gatcatggtt
     9421 cactggaaac agtgtgttcc cttggacatg ggaatacaat gtaacagatg ttgccagtgg
     9481 tgcttctgct gctggtgatg caaccacagc tgccagcgga gcaggggcaa gtgccggaga
     9541 agcagcgact caagctgcac attttggttt gagttatgca tacggtgaag aaccaatggc
     9601 tgtgattgag aagtcaccag actggttcac aaagatcatg caagtgatca tgccaaacaa
     9661 tgatgtagca atgttcttcc aaaaattcat ggtattcttt gaaattgcct tggcattagc
     9721 attgattgct ggattattca catggctatg cagtgcgaca acgattgtat tggtgatcac
     9781 gttctgctta tcaggcatgt tttactgggt aaacatttgg ttcatttttg ttgctttcgc
     9841 attgatgaac ggttccggac gtgcgattgg gttggatcgc tgggtaattc catggctaca
     9901 acgtacacta ggtaattggt ggtacggtag accaagttcc cagtacggca agaaataaat
     9961 agataaagaa cgttatttga atgcaagatt tcaatagaaa ggggctgaga caaaccgtca
    10021 cagcctcctt tttatgtaaa attaaaggaa taagatgccc gagataagcc tccatgagca
    10081 agacacaggt gtcacaacct ctgttacaac ctcttgttat aaacggtgtt ccagaagtag
    10141 cttcttcgaa aaataagccg aaatttcaca aaaacttgaa aagtaatttt cgtaaattcc
    10201 ttcttatttc ttgaagttgc ccacttctgt cacaacctct tgttataaac ggtgttccac
    10261 ctgctgcttc tcggtaataa gcccaaagaa ttagaaaaca tggaaagctg ttttctattc
    10321 ttcgttctta ttactcaaag caagacacag gtgtcacaac ctcttgtttt tatgctaaaa
    10381 ttgggtgagt taatctggaa aaaggagaac aaaaatgagc cttaaagaat ttgctgaaaa
    10441 aagtctgctc aaaccttggg atgtttttat tattttgctt ttgactgttc tttctttttt
    10501 accagtcatg atttttggtc ttcagcaaaa caccgcaaca gcagaaaaag aagcggtact
    10561 gcgtgtggat ggcacagaaa tcaaaacatt ccctttgatg gctggagaag agagcaacac
    10621 atatacttac aaagatgaac acggagatta taacttgatc gagatagatg gcgatcgagt
    10681 acggataaaa gaagcagatt gcgatgacca gatctgcgta cgtcgaggct gggcaacgaa
    10741 aaatggcgaa accatcgtct gtttgccaca taaattagtg atcgaagtcc gttcaacaga
    10801 tggaggtgac gaagacagtc tgatttatta gaccttcaca caccatgagt aaattacaaa
    10861 aaatgattta tatttcgcta cttgttgcac aaggagtgat cattggttta ttggaaaata
    10921 tgatccctta tcctttttct tttgcgcctg gtgcaaaact aggcttagcc aatctaatca
    10981 cgatcgttgc catttttacc atgaaaaaac gagacagctt cttactactt tggctacgac
    11041 tattcctgac tactttgcta ggcggaacga tttccacatt cctttatagt atgagtggtg
    11101 ctttgcttag ttattttgga atgttattgg tcaagcaatt aggaccaaaa cgtgtcagta
    11161 tcatcggaat tagtgcggcc ggcggtttca tgcataatgt cggacagctt ttgatggctt
    11221 ctttcattgc acaatcatgg acagtcatgt tgtacttgcc agtcctttca gttttaggga
    11281 ttttatcagg gattgcaatc gggatcgcag cgaattatct tttacgacat gtgcagacac
    11341 ttcaacgatt ccaaaaagat tatgagcagg gaaccaaaca aaaatggagc ccaatgttta
    11401 ccaaaaaata atcgtcacac aaggagtttt tttatgaagc tacatccaat gtgggaaaac
    11461 tatcctgctt taagtaaaga attgacagaa acactaaaac ggatggaaga aactgtccgc
    11521 ctgaaaaaca aacctgtcga acaagcaatc aaagaaacga tccatgccgg cggaaagctg
    11581 ttgcgtcctg cctatcagct attgttctca caatttggac cagagcaaga tcgagacaaa
    11641 gccatcgctt tggcagcttc tatcgaaatg ctccatacgg ccacactgat ccatgatgat
    11701 atcgttgatg aagcggatct gcgacgtggt cagcctaata tccgtagtca gtttggtaat
    11761 gtcgtttctg tctatgcagg agactattta tttgtctgct gttttaaact attgtctgat
    11821 tactccactt ccttgaaaag tctccaattg aattctcgca gtatggaaaa agtattgaat
    11881 ggcgaattag ggcagatgga tgatcgctat aattatgaac tttctgtaaa agaatatttg
    11941 gaaaatattt caggtaagac agctgaactg ttccaattaa gttgttctgt tggcgctttt
    12001 gaaagtggaa caagcgagcg cttcgcaaaa aaagcaggag atatcggact ttctatcggg
    12061 atggcctttc agattatcga tgatatttta gattatacaa aagagagcca ggaaatcggc
    12121 aaacctgtac tagaagacat gcgtcaaggg gtctattctt taccgttgat ctactctttg
    12181 caaaaaaaca aaccaaagct actaccttat ttagagaaga aagcggcaat gacagaagaa
    12241 gatgtcgacg ccgtacgcaa aatcgttgaa catacaggag gcgtggaaga agctaggaag
    12301 ttagcggcct cttacacaga aaaggctttg aaggaaataa agaaattacc tgccacctct
    12361 cttcgaacaa aagagaatct cttttccctt acccaactga ttttggatcg caaagactga
    12421 atagggaaag gttcgatacg taaataaatt tctcagaagg aaatctacgt gttttttgac
    12481 aaattgaatt gtagttcttg tatttatgat aaaaagcaaa aataaagagc aacgtccgtt
    12541 agcgacgttg ctctttttta tcttgaatat ttttttacct tttcgatttc ttataactca
    12601 taaaagtgat tcttaaaccg attacaagta atataacgcc tgcgagtgtt atccaattat
    12661 tcgattgact tccagtttta ggaagtgctc tttttgattt gtttccattg tcattgcttg
    12721 ttttattagt taagttaaag ttcgtcctgt tatccgaact tgtttttgag aaggagacac
    12781 ttgcatgacg tgtatcgctt gtggtagacg acgtgtctgt actatcgctt gaagttgatg
    12841 aatctgagct atcacttgtg gtagaggatg tatctgtact gtcacttgaa gctgacgtgt
    12901 ccgtgctatc acttgtagtg gtagatgaat ctgagctatc acttgtggta gaggtagtat
    12961 ctgtactgtc acttgaagct gacgtgtccg tgctatcact tgttgtggta gatgtatccg
    13021 tactgtcgct tgaagctgac gtgtccgtgc t
//
LOCUS       DASJTC010000078        12765 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.079, whole genome shotgun sequence.
ACCESSION   DASJTC010000078 DASJTC010000000
VERSION     DASJTC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.079"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            67..2232
                     /gene="acm"
                     /locus_tag="U4B19_002089"
     CDS             67..2232
                     /gene="acm"
                     /locus_tag="U4B19_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEN2077817.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2504..2989)
                     /locus_tag="U4B19_002090"
     CDS             complement(2504..2989)
                     /locus_tag="U4B19_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEN2077818.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3079..3424
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3469..4110
                     /locus_tag="U4B19_002091"
     CDS             3469..4110
                     /locus_tag="U4B19_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEN2077819.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4129..4764
                     /locus_tag="U4B19_002092"
     CDS             4129..4764
                     /locus_tag="U4B19_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEN2077820.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4782..5597
                     /locus_tag="U4B19_002093"
     CDS             4782..5597
                     /locus_tag="U4B19_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEN2077821.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5916..7352
                     /locus_tag="U4B19_002094"
     CDS             5916..7352
                     /locus_tag="U4B19_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2077822.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKSSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7541..9973
                     /locus_tag="U4B19_002095"
     CDS             7541..9973
                     /locus_tag="U4B19_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEN2077823.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQLSFVPI
                     DSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQDYELPT
                     VDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEVQPAVG
                     VKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIEAQPNH
                     PDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRAKPHEV
                     KLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAATGVRNI
                     SGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMARAAGI
                     HMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGDMLFLP
                     MGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQPQDELF
                     EEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKVFIEQE
                     ETSETLPDDNDM"
     gene            10203..11270
                     /gene="rlmN"
                     /locus_tag="U4B19_002096"
     CDS             10203..11270
                     /gene="rlmN"
                     /locus_tag="U4B19_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEN2077824.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11463..12566)
                     /locus_tag="U4B19_002097"
     CDS             complement(11463..12566)
                     /locus_tag="U4B19_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2077825.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4364 a         2143 c         2718 g         3540 t
ORIGIN      
        1 agaaaggatt tttgagagat gatatagtag gttttatttt tagttaaaat aggggagata
       61 gaaaaaatga aaaagtgtag aaatattatt ttatcaatgt tatttattat tactaatata
      121 acatcactga ttcctgttca tgtctatgcg gatgcaggca gagatatcag cagtaatgtc
      181 acttcgttga ccgtggaccc aacgaatatt acagatggag gaaacataaa agtaaaattc
      241 tcgtttgacg aaaaaaaaca aaatatccaa ccaggtgatt atctttggat caattggcca
      301 agtgaaggaa atattcgcgg tgaaggattc caaaaagaaa tcccattaat gattgaaaat
      361 aaaaatgttg gaactttaac ggtcagaaaa gattctgcgc aggttgtgtt taatgaaaat
      421 attaaaaacc tagactcagt agaaggttgg gggcaatttg agatacaggc cagaaacgta
      481 accgataccg gtgaagaaaa tacgggacca ttcaccgtga cgagcggtga taaaacagct
      541 actgtaaatg tgacaaagcc agcttctggt tcttcttcta gcgtttttta ttataaaaca
      601 ggagacatgc tacctgaaga tactaagcac attcgatggt ttttgaatat taacaataac
      661 ggcacttatg ttgaacaacc agttaaaatc agtgatgaaa tccaaagtgg acaaagatta
      721 gatccaagta cgtttgaaat taatcaaata cacttaggtg aacagaaggt ttatagagga
      781 gaagagggaa tccaacaatt tttacaagat ttccccagcg ctacatttaa tttttcagta
      841 actgataatt acattgaaat aactatacca aaaaactttg tcaatcttag gaaaataatg
      901 gtgagttata agacgatcat agaaaaccca gaacaaataa attttgagaa tcattcggaa
      961 gcttggttta aagaatttaa taaacctgct gttgatggag aaagtttcaa tcatacagtt
     1021 aaaaatattt cagcttctgg tggagtcaac ggtacagtta gaggcgaatt gaagattttc
     1081 aaatatatca atgacacaga gattggtatc ccaaatgtga ctttcgaatt aagacgagca
     1141 gatgaacagc ctattcaagg acagtctagt atcctgttga cttcgaatga acagggagaa
     1201 gctagtatca aaggcctgca agtaggagac tatgttgtaa aagaaaaaga agcacccaat
     1261 tggattgatt ttgatccact aagttcaaat gaactcaaat tctccataaa cgaaaatgat
     1321 acagagggag tcagtttacc tatttataac aagaaaaagg ttacgaacat cacagcaact
     1381 aaaatttgga acggtggaac cactccaaga ccgagtattt attttaaatt attcagagca
     1441 agtacaaaca attgggaacc agttcctgat gcagaaacaa aacgattaga caatggaata
     1501 acttcagtga cttgggaaga tatccaacag tatgatgata gtggaaacga atatacgttt
     1561 aaagtgcaag aagtagatca aaacggaaat gattatgtcc cttcaggtta tcaaaaaatt
     1621 gaaaacgggc tagtcgttac aaatgagaat aaagaagtca tttcagtctc aggacaaaaa
     1681 acatgggaag acaatgagaa ccaagacggc aaacgaccga cgacgatcac agtaaatcta
     1741 ttagccgacg gtcaaccgat ccaacacaaa gaagtaagtg aaaaagatga ttggcgctat
     1801 cgtttcacaa atctgccaaa atacagagac gggcaagaaa tcatctatac tgtaacggaa
     1861 gacaacgtgc ccgaatacag tacgacgatt gacgggtata acatcaaaaa cagttataca
     1921 ccggaaaaaa caaatatatc tataccaggt agccatataa caccgtggaa aaattttcca
     1981 gagaagacag agagagagaa aattagaaat ctattctctc aaaaattgca aatatttgct
     2041 catacaggag aatccagcga acaaagaagc aattatatta gtaagagagc tgtacctaaa
     2101 cagataacaa ataaatcaaa aagcattctt cctaaaacaa gtagtaaaaa atctagtttt
     2161 gctgtaatca ttggtttatt gatagtaact atctgtgctg ggatattctt acaaaaatat
     2221 aaaaaagttt agttagaata taatattttt tgttaaatat gactgaaatc aaaagaataa
     2281 aattccgatt tataatcgaa aaaagaagca tttttatata tgcttctttt ttcgtagaat
     2341 caataaatat ggatttggct aactatggaa gaatactcgt attagttttc agcgttcata
     2401 ctagttgtta cattgtttga ttggccaaaa gaatttctga tatattttgt gctaaataaa
     2461 gaaaaagacg cttgaattgg gaaataagaa aactactcta tcattacaaa ttttcataaa
     2521 ctggcagatg ttctaagtaa acaagtatct tttcaaagtc ggaaaaatcc ataatcaacg
     2581 tagtggtatt gatatttgga tgaaagccta cggtatcttg acgatcaata gcagcatcga
     2641 tcacgacttg gatttttttc tctttgtcgt gcatcaatgc taatggagtc actgtacctg
     2701 ctggaacgcc taggaggtca ggaagctgtt cttcagaaac aaaagataag cgcttttctt
     2761 ttaattgttc agctagttgt tttaaatcgg cgtgcttttc atcaggcaag atgacaagat
     2821 agatttgttt tccttttctt gatttcaata atagattttt tacttgcgga ccaggtaggg
     2881 aaaaagggac atttttgact gacgtaatcg caggatgatc gactttttga aaagagatac
     2941 ccagtttttt tagtagttca taagcttctt gttcggttgc acgactcatc gcgcatcacc
     3001 ctcctttttt tattagtgta tcataaaaaa gagttgacaa atgaaaaata atcatgtaaa
     3061 ttacacgtaa agttaaaaca aatgacatcg aaaagaagag tagccgttag gtgtttttag
     3121 agagtctctg gttggtggaa agaggcagca gttaaccgtg aaacacatct tggagtcgat
     3181 tttctgaagc gtatagtagg aaaatccggg gagcccgtta cagctgcggt cgttgctaga
     3241 tgaccagata aggctggctt ttccgtgagg agtcggtaaa aaaaggtgga accacgtgat
     3301 taacgtcctt tgatcttcgg atcaaaggac tttttgtttt catctgaacg gcaacgactg
     3361 tgtgaggttt tttctgcaag gaaaaaataa acagaggtgg aaccacgtat aatacgtcct
     3421 cttggcattt gccaagagga tttttttatt ttaaaggagg aaaagaagat ggagtatttg
     3481 attgaagtta tgccgcaatt attagtagga gcactcacaa cattgaaatt atttttattt
     3541 accttgattg gctcattacc attagggatt gttttagcat taggattgtc tttgcatttt
     3601 aagccgctac gctacttgct tcaaacatat gtatgggtta tgcggggaac accgctgttg
     3661 ttgcaactga tttttatttt ttatgggttg ccgacgattg ggatcgtttt tgatcgtttc
     3721 gaagcagcac tcattgcctt tatcttaaac tatgcagctt attttgcaga gatctttcgt
     3781 ggagggatac aatcgattcc tcaaggtcaa tatgaagcag cgcaagtttt gcaattaagt
     3841 ccctttcaaa cggtgacgaa aatcgtcatc ccacaagtga caaagattgt tttaccttct
     3901 gtagggaatg aagttatcaa cttggtcaaa gattcttcct tgatctatat attaggttta
     3961 ggtgacgtaa tgcgtgctgg gaaaatcgcg atggaacgcg acgcgacttt actgcctttg
     4021 ctaggcgtgg caatcatcta tcttacattg actggtattc taacaatcgt aatgaaacaa
     4081 gttgaaggaa aattaaatta ttaccgctaa ctgataggag gaagaaatat gattgaattg
     4141 aagaaagtga acaaagcatt tggtgaaaaa aaaataatca aagatttgaa tctaatcatt
     4201 ccagatggga aaacattagc tatcgtaggt ccttccggtg gcggtaaaac cacgctttta
     4261 cgtatcttgg ctggcttaga aagttctgac agcggagaat tttatttaga cggtgtaccg
     4321 ttcaacccat tcgagcttaa aatgaaagaa caagttgtag gagtcgtatt ccaagacttc
     4381 cagctatttc cacatctgaa tatttttgaa aacattacct tagcaccaaa aatggtattg
     4441 aaacagacaa aagaagaata caccaaaaaa gcagaagaat tagcggcttc attagggatt
     4501 agtgatttat tgaatatcta tccttatcag ctttctggcg gacaaaaaca gcgggtagcg
     4561 attgccagag cattggctat gaatccacgg gtattggctt atgatgaacc aacaagtgct
     4621 ttagatcctg aattacgcca gcaagtcgag cgtttgatcc tatcattgaa agaagatggg
     4681 gtaacccaaa ttattgtcac ccatgattta gtatttgcag aaaatgtcgg tgatcaatta
     4741 ctaaaagttt ccccaattca ttaagaaagg agaagataga gatgaaaaaa accagttttc
     4801 tattcgcttt gatcgcaggt cttcttcttt ttgcaggctg ttcgaacaaa aaaacttcag
     4861 cagataattg ggcgactatc aacgaagaga aaaaaatcgt tataggttta gacgatacat
     4921 ttgttcctat gggatttaga gataaagacg ggaagctcac aggatttgat attgatttag
     4981 ccaaagcagt ctttgaacaa tatgggatta cggtagactt tcagcccatt gactggtcaa
     5041 tgaaggaatt tgaactgaac aatggaacga tcgatttgat atggaacggc tattccaaaa
     5101 cgccggcaag agaaaagaaa gtgcaattta caaaacctta tatggaaaac gatcaagtgc
     5161 tgatcacacc taaagaatca ggaatcacaa actttggaca aatgaataat aagagattag
     5221 gtgcgcaaaa tgggtcatct ggttatgatg tatttacgaa acaaccagaa gtattaaaag
     5281 agatagtcaa aaaccaagat gctgttttgt atgatagttt taatgaagca ttgatcgatc
     5341 tgaaaagcag acgggtcgat ggtctgctga tggataaagt atacgcagac tattatttga
     5401 aacaaagaaa cgaactagaa gacttcaaca taacaaaagg ttcttatgag agtgaggatt
     5461 ttgcagttgg tgcacgcaag tcagatagta cattagtgaa aaaaatcaat gaagccctag
     5521 atcaactaga gaaaacaggt gaattccaaa aaatatccga taaatggttt ggcgaagatg
     5581 ttacgccgag aaattaacaa ataggggact gtgacaaaag acgtgtcaca gtcccttttt
     5641 ccgaataaac agtgttctcc tgccaactcc ttgtgactcg gagctgaatg gcaggctcac
     5701 aacctcttgg taaacggtgt tcaaaagcca gtttttcggt attaagccaa atttatgcaa
     5761 aaatgtagaa agccattttc acaaaatttt tcttactact cgaaactaag cgactttttc
     5821 acaacctcct ttacataatt ttattttgtt gtggaagggg tttcgtgtta ttctaaaaga
     5881 ggaacgattg aaggttatag gaaaggaaga gatgaatgga acacaaagaa cttacctcat
     5941 ttccaaaaga ttttctgtgg ggctctgctt ctgcagcgta tcaagtagaa ggagcttggc
     6001 aagaagatgg gaaaggagaa tcagtctggg atcgatttgt acggatacct ggaaagacat
     6061 ttaaaggaac gaacggtgat ttggcagtag accactatca tcgttataaa gaagatattg
     6121 ctttaatgaa agaacaaggt ctgaaagctt accgcttctc tgtagcttgg acacggattt
     6181 ttcctaatgg acgaggagaa gtcaatcaag ctgggctcgc tttttatgag agattgatcg
     6241 acgaattgat tgaaaacgat attgaaccta tgttgacttt atatcattgg gatcttccac
     6301 aagcattgca agatgaatat aatggttggg aatcacgtca gatcattgaa gatttcactc
     6361 attatgcaga aacattattt gaggcattcc gcgggaaagt ccattactgg attagtttga
     6421 atgaacaaaa tatcttcact tcattaggtt atttgttagc agcacatccg ccaggtgtga
     6481 cagacccgaa acgaatgtat gaagttaacc atattgctaa tttagcaaat gcatctgtca
     6541 tcaataaatt tcacgagtta gaaatgcctg gaaaaatcgg accaagcttt gcgtatacgc
     6601 caaattatcc aattgacagt aatcctgaaa atgttctggc tgctgaaaat gcagaagacc
     6661 tgatggccaa ttattggatg gatgtctata tgtggggaaa atatccgatt gctgccatga
     6721 ggttcttaga agaaaaagga tgggcaccaa cgattgaagc tggcgatgca gaattgctag
     6781 aatctgctaa accagatttt cttgggatca attactatca aacggcgacg aatgcattca
     6841 atccattaaa cggagttggt gctggaaaaa tgaatacgac aggaaaaaaa agttcatcag
     6901 aagaaactgg agttcctgga atgtataaaa aagttgagaa tccatttgtc gaacgaacaa
     6961 attgggactg ggagattgac ccagaaggat taaggatcgg actgcgtcgt atcacaagcc
     7021 gttaccgcat gccggtactg attacggaaa atggcttagg agagtatgac aagttgacag
     7081 aagataaaca aatccatgac gactatcgaa tcaattactt acaaagccac gtcaaagcca
     7141 tcaaagaagc aatttcggat ggagcagaag tcctaggcta ttgcacctgg tcttatacag
     7201 atttgcttag ctggctgaac ggttatcaaa agcgatatgg atttgtatat gttgatcaag
     7261 acgaaacaca agaaggatct ttggaacgat ataagaaaga cagttattac tggtaccaaa
     7321 aactgattga agaaaatgga gagaaccttt agttcacata cttaaaacaa gaaggtacgg
     7381 ctggatatgg ccgtgccttt tcttgataaa atcatttaga aattaagaaa aactgtaaaa
     7441 taagctgaaa aatgaaagaa agcgtcagtt ctttcaaaca aaactttagt tcccttactt
     7501 ttttttgtat aatgaaactt gatgaaatga gggataataa atggcacaaa agaaacggcc
     7561 tactaaaaaa aggaaaaaga cgaaaaaaac gaaaaagcaa aaacagcagc aggaacaaat
     7621 gctgacgatt ggtgttgggt ttgccttgat tttatttgct gtcttcggct ttttaaaatt
     7681 aggtttttta ggaatattga ttgcaaacgg ctttcggatc attgcaggaa atacttacca
     7741 aatcctctgt cttttattag ccgtattagg attttggatt gtcattaaga atacagagtt
     7801 ttcaattggt aaaaaccgac gctggtttgg cggcatactc ttttatttcg gtatcttatt
     7861 attgttacat gcccatttat ttggcaaact ccatactggg gaaccaaata tcatggggac
     7921 gacatgggat ttgttagcca gtgatataaa acaaagtcaa gtagacaata acgttggtgg
     7981 tgggatgatt ggagcaatcc tttatcattt tacttacttc ttgattgcac agcctggaag
     8041 ttatttagca gcagtgctat tactggcagg aggagcattc ctgttcagta atctggaagg
     8101 ctatcagctg ttaaatggta tccaatcaat tggagaacga gtacaggaat tattggaagg
     8161 tgatcctgcc aagcaagctc gaaagcaagc ggcaaaggaa gagagaatga aacagcgcgc
     8221 agaagcgaaa gaagcaaggc gcttagctgc aaaagaagct gcagaatatg agaagaaaaa
     8281 agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg caggaaccag aaaatacaga
     8341 gccggagcag ctaagttttg ttccaatcga cagttttcaa gaaaatatcc atcctgcgaa
     8401 tctagagaaa ccagtaccag atacaccaaa acagaccaat acagcagaag gtttttcaga
     8461 tgagttgcca gaggatgacg gtacctcatt agaatttgag attgaagcag aacaggaaaa
     8521 tcaagactat gaattaccga cagttgattt gcttgattct ataccaactg tggatcaaag
     8581 tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt tccaaagttt
     8641 cggtgtcgat gccaaggtcg tcaaagccag cttaggacca tccgtcacaa aattcgaagt
     8701 ccagccggct gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg atatcgcgct
     8761 tgctttagcg gcgaaagatg tccggatgga agcaccgatt ccaggtaaat cactgattgg
     8821 tattgaagta ccaaatggaa agatcagtat ggtttccttt agagaaatca tcgaagcaca
     8881 accaaaccac ccagataaat tactagaagt acctttagga cgtgatgttt ctggacgggt
     8941 acaaacggct gatctttcga agatgcctca cttactcgtg gctggttcga ctggtagtgg
     9001 gaagtcagta gcaattaatg ggattatcac aagtatatta atgcgtgcta agccccatga
     9061 agtaaaactg atgatgatcg atccgaaaat ggttgaattg aatatgtaca acggcatacc
     9121 tcatctgttg acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct tacaaaaagt
     9181 agtacaagaa atggaagaac gatatgaaaa attcgcggca accggagtaa gaaatatttc
     9241 tggttacaat gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac atccaacctt
     9301 accattcatc gtagtgatcg tagatgaatt agctgatttg atgatggtag ccagcaatga
     9361 agttgaagat gcgattattc gtttagcgca aatggctcgg gcagcgggga ttcatatgat
     9421 cctagctact caacgtccaa gtgtggatgt tatcacagga atcatcaaag caaatgtacc
     9481 ttccagaatg gcatttgcgg tatcaagcgg tacagattct cggacgatca ttgattctaa
     9541 tggggcagaa aagctcttag ggcgaggaga tatgctattc ttgccaatgg gtgagaataa
     9601 accgatccgt gtgcaaggag cattcatttc tgaccacgaa gtagaacggg tcgttcaatt
     9661 tgttacagat cagcaagaag ctcattatga agagaaaatg atgccgacag atgaagttga
     9721 aacgacaggt gctccagaac agccacaaga tgagctgttt gaagaagcaa aagcacttgt
     9781 cgttgaaatg caaacggcaa gtatttccct tctccaaaga agatttcgga tcggttacaa
     9841 ccgagcagca cgtttagtag atgaactgga agctcatgga gttgttggcc cgtcagaagg
     9901 aagcaagcct cggaaagtat tcattgagca ggaagaaaca tcagaaacgt tgccggacga
     9961 taatgacatg taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa taaaaagaca
    10021 aggcatgatg ataccaaaag aaaaaatggt ttttttccat cttttctaat acggtatgat
    10081 gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg tgaagtattg
    10141 cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata
    10201 ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat tggtttatag
    10261 aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg
    10321 taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc
    10381 attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat ggtacagtaa
    10441 aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg agacaagaat
    10501 atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt acgttttgtg
    10561 caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt gcacagatca
    10621 tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca catgtcgttg
    10681 taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatca
    10741 tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc actagtggat
    10801 tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg gctatttcct
    10861 tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa
    10921 ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt
    10981 ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag
    11041 atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca
    11101 gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat
    11161 tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag
    11221 cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta
    11281 ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta
    11341 aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc
    11401 atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt
    11461 atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg
    11521 tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca
    11581 tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg
    11641 tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa
    11701 tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga
    11761 aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca
    11821 gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa
    11881 acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta
    11941 ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg
    12001 tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg
    12061 ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag
    12121 gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag
    12181 atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact
    12241 ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact
    12301 gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca
    12361 taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct
    12421 acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt
    12481 gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa
    12541 tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta
    12601 aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga
    12661 caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa
    12721 aacgaacaag tacaaaaaca ttgaaaacga caataatgaa ttttt
//
LOCUS       DASJTC010000079        12426 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.080, whole genome shotgun sequence.
ACCESSION   DASJTC010000079 DASJTC010000000
VERSION     DASJTC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12426
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.080"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..662)
                     /gene="frr"
                     /locus_tag="U4B19_002098"
     CDS             complement(105..662)
                     /gene="frr"
                     /locus_tag="U4B19_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEN2077826.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(665..1387)
                     /locus_tag="U4B19_002099"
     CDS             complement(665..1387)
                     /locus_tag="U4B19_002099"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEN2077827.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1523..2404)
                     /locus_tag="U4B19_002100"
     CDS             complement(1523..2404)
                     /locus_tag="U4B19_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEN2077828.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2503..3285)
                     /gene="rpsB"
                     /locus_tag="U4B19_002101"
     CDS             complement(2503..3285)
                     /gene="rpsB"
                     /locus_tag="U4B19_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEN2077829.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3644..4123
                     /locus_tag="U4B19_002102"
     CDS             3644..4123
                     /locus_tag="U4B19_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEN2077830.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4340..5431
                     /gene="phnW"
                     /locus_tag="U4B19_002103"
                     /pseudo
     CDS             4340..5431
                     /gene="phnW"
                     /locus_tag="U4B19_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            5424..6209
                     /locus_tag="U4B19_002104"
     CDS             5424..6209
                     /locus_tag="U4B19_002104"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEN2077831.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6555..8246)
                     /locus_tag="U4B19_002105"
     CDS             complement(6555..8246)
                     /locus_tag="U4B19_002105"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEN2077832.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8668..9615)
                     /gene="arcC"
                     /locus_tag="U4B19_002106"
     CDS             complement(8668..9615)
                     /gene="arcC"
                     /locus_tag="U4B19_002106"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEN2077833.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9730..10749)
                     /gene="argF"
                     /locus_tag="U4B19_002107"
     CDS             complement(9730..10749)
                     /gene="argF"
                     /locus_tag="U4B19_002107"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEN2077834.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10840..12069)
                     /gene="arcA"
                     /locus_tag="U4B19_002108"
     CDS             complement(10840..12069)
                     /gene="arcA"
                     /locus_tag="U4B19_002108"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEN2077835.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3436 a         2558 c         2205 g         4227 t
ORIGIN      
        1 tttaaaaact gaataatttt ttcttttttt aatttttaaa taaaagaaat ataaataaaa
       61 acagaggaag cggaatgatc cgcccctctg tttttatgat tttattaaac ttccaataat
      121 tctttttctt tatcagcagc gatcttatct acttctttaa tgctgttatc tgtcaatgtt
      181 tgtacgtctt tttctaatcc gcgcaaatca tcttcggtaa tatcaccatt tttttgttgt
      241 tttttatatt catcgatcgc atcacgacgg atgttacgaa tcgcgatttt tgcattttct
      301 gcttcttttt tcacatcttt agctaattct tttctgcgtt cttctgtcag ttgcgggata
      361 actaagcgaa tgacatttcc atcattggtt ggactgattc cgatatcact tgccataata
      421 gctttttcaa catcttgaag aatgctcttg tcaaaaggtg tgatcattaa aacacgtgct
      481 tctggaattt ggattgatgc catttggttc aatggtgttg gcgcaccgta gtagttcaca
      541 gtaatacggt ccaaaaggct agcgttcgca cggcctgcgc ggatttgccc aagttcacgt
      601 tgaagatttt gtgctgcttt tttcattttt tcttttgctt ctgtcataat tgcatctgcc
      661 atttttattt cccccttaca gttgttccga tattttcgcc taagattgca cggcgaatat
      721 ttccgtgttc atttaaattg aagacaagca gtggaatatc attatccatg cttagtgaac
      781 ttgcagttga atccatgact tgtaatcctt tggcaataac ttccagatga gtcagttctt
      841 caaattttac tgcatttgca tctactttcg gatcagcaga ataaacaccg tctacattgt
      901 ttttagccat caggataaca tctgcgccga tttctgccgc tcgtaatgct gctgttgtgt
      961 ctgtcgagaa gtacgggttg ccggtaccgc ctgcaaagat aacgatccgt cctttttcta
     1021 aatgacgttc tgctttacgg cgaatatatg gctctgcgat ttgacgcatt tcaatcgaag
     1081 tttgcacgcg tgtcggtaca cccacgtttt ccaacgtatc ctgtaaagct aaggcattca
     1141 taactgtagc taacataccc atgtaatcag cttgagcacg ttccatgccc atttgtgctc
     1201 cgatttgtcc gcgccagata tttccgccac ccacaacgat tgccatttca acgcccagct
     1261 cgtgaacttc tttgatttct tggatgattt ctttgattac tggtggtttg attccaaatc
     1321 ctgcatcccc agctaaagct tctccactca attttaagac tacacgctga tatttcggtg
     1381 tgaccatgtt cattcctcca atattttcta ttgccatttt accatactta gatgatttcg
     1441 acactctaag actggttttt cttgttttgc ttaaaaaaag ggagcgcaca aaccgtgcgt
     1501 tcccgtctgt tattcaacag aattattttt tcacttggct cataacttct tctacaaagt
     1561 tatcttcgcg tttttcgata ccttcgccaa cttcgaaacg tacgaatgtt ttaacagttg
     1621 cgccttttga tgcaacgtat ttttcaacag tcatatcagg atctttaacg aatggttggt
     1681 ccaccaaagc gatttctgct ttgaatttct tcaagcgtcc ttctaccatt ttttcaacga
     1741 tattcgctgg tttgccttcg ttcaatgctt gttctgtaag aacagttttt tcatgttcta
     1801 attcagcttc tggaatttgt gattcgttta cataacgagg attgatagct gctacgtgca
     1861 tagctacgtc tcttgcaact gtttcgtcag ttgttccatc tagaacagtc aatacggcaa
     1921 tacgtccgcc catatgcaag tatccaccaa atgcagcatt gtcgtctttt tctacaactt
     1981 caaagcgacg gaagctgatt ttttctccga taacttgagt tgcttcgatc aagtcatatt
     2041 caattgtacc tttatctgtt ttgattttca ttgcagcttc catgtctgct ggtttgtttt
     2101 cagcaacaag ttctgcaatt tcttttacta aatcttggaa catttcgttt ttagaaacaa
     2161 agtctgtttc tgagtttact tcaacgattg ctgcagtgtt acctttcaca gcaactgaag
     2221 ccaatccttc agctgcaatg cggtcatttt tcttcgcagc ctttgccatt cctttttcac
     2281 gtaaaagatc aactgctttt tccatgtcgc cttctacttc aactaatgct tttttcgcat
     2341 ccatcattcc tacgccagtc atttcgcgta gttcttttac cattttagct gtaacgtctg
     2401 ccatgtttaa ctcctcctgt gtgtttttct ttttaaaaaa agctgcttca aaggagagtg
     2461 aggcttgtcg cccagccttt gaaacagctc catcattcaa aattattctg ctgaagaatt
     2521 gtcgccttca acaacatcta cgatttcttc gattgatgtt gctgtgtttt cttctgtgaa
     2581 atcttcttca actacttgat cttcaccttg gtttccttcg atgaaagcat cagccatttt
     2641 tgaagtaatc aatttaacgg cacgaattgc atcgtcgttt gaagggatta ctacgtcgat
     2701 ttcatctgga tcgcagtttg tatcaaccat agctacgatt gggatattta atttgtgtgc
     2761 ttcttgaaca gcaatacgtt ctttacgtgg gtcaacgatg tacattacat ctgggattct
     2821 tggcatatca gcgataccgc ctaagaattt ttcaagacgt tcacgttctt tgttcaaacc
     2881 agcaacttct tttttaggta gaacttcaaa agttccatct tcttccattg cattgatttg
     2941 tttcaaacgg ctgatacgtt tttggatagt atcccagttt gtcaatgttc cgcctaacca
     3001 acgatggtta acgaagtatt gtcctgcacg agttgcttct tctttgattg cttcttgtgc
     3061 ttgttttttt gtacctacga ataatgctac gccgccttct tcagcaacgc tcttcatgta
     3121 atcataagct gcatctacta atttaactgt tttttgcaag tcaatgatgt agattccgtt
     3181 tctttctgtg aagatatatt tcttcatttt tggattccag cgacgagttt ggtgaccaaa
     3241 gtgtacgccg gcttctagta attgtttcat agaaattact gccatgtttg tttcctccga
     3301 tttggtttta tttttcctct tcttatcttc taccttgccg gcaaactttt tcaagcacct
     3361 accgacaatc caataagaat gtggatttgc tgatgcataa agcaccctca gctattatac
     3421 agacaaaaga tccgtatcgc aagagcaaat atacttttct gctcaacatt caatagttta
     3481 cctgaaagat aaaaagaatt caagaaaaac agtagatttc tctttgtttc tttattatcc
     3541 tttttccata aaataatttt aggtataaaa gacgaatatt attgggcaat ccttcttttt
     3601 tttgttatga tacatgtgaa atctttcgta aaggagaaag aaaatgcgca aagctcaaag
     3661 acatttgctg attaaacaag tcattgaaga attcccgatc cgtacgcaag aagaactatt
     3721 aaacaaactt gggcattatg gcgttaccgc cacgcaggca acgatttctc gagatatccg
     3781 tgatctaaaa atcgtgaagg cacctgatga aaatggcgtt tcccgcttcg tattgtttcg
     3841 cgaaaaagga actcctgaga ctaggactga ggaagaggat cggctggttc aaatgattga
     3901 agacattgta ttgaaagtcg aacgagtcca ctttttgacg atcgtcaata ccttgccaga
     3961 caatgctcat ttatttgcat ctgttttaga tgagatcaaa cctccctcta ttgtcagtac
     4021 aattgctgga ttcgatacga tcatggtcat ctcggaaaat gaggaggacg caaaacgagt
     4081 agaagaattt cttcaccatc caactgaagt atcacttttt taatcagagc acgtattcct
     4141 aatatggaat gcgtgttttt ttattttcaa tattttcctt attaaataaa cctttacact
     4201 ctttacaaaa atacatcatt ttttattcaa ttttttcaac accttgattg aatcacaata
     4261 atcatcattt atattggatg ttaacaagag cagcttcttg ctctttgtgt atttcaaaaa
     4321 agaaatgaaa ggaaatcata tgtataaatt attaactcct ggaccattga cgacttctga
     4381 aacagtgaaa aatgctatgc tgatcgatca ttgtacttgg gatcaagaat acaaagaaat
     4441 tacacaatgg ataagaaaaa aattattgga tcttgctcaa gtatcggaag gaatatacac
     4501 aacggtctta atgcaaggta gcggctcctt tggggtagaa tcggtattaa gttctgtgat
     4561 caggccagaa gatactgtac tgatttgttc aaacggcgca tatggacaac gaatgatcaa
     4621 catgtgcgaa tgtctgaaac tgaactatac cgtttatgag gtatcgtaac atcaaatcct
     4681 ccaagctcat gtgattacac agctgattga agagaatccc tctatctccg cactagcaat
     4741 ggtccatagc gagactactt ccggtatttt gaataatttg gaagagattg ctgctgtcac
     4801 acaagaaaaa gatcttttgt ttatagtgga cgcgatgtca agctttggtg ggataccgat
     4861 tcgtgtagcc gaactaggga ttgatttttt gatttccagc tcaaacaaat gtattcaagg
     4921 tgttccaggc ttttcttttg tacttgctaa taaagcggaa ctagaaaagc gaaaaaatgt
     4981 gagtcgaagt ttgtcacttg atctatatga ccagtaccgc gtgatggaaa acgagaatgg
     5041 caaatggcgt ttcacttccc ctacccatac ggttttggct tttcaacaag cgctgaagga
     5101 actagaggca gaaggaggaa tcgaaactcg atattcacgt tatctcgcca ataatcatgt
     5161 actacgacaa aaaatgacag agatggggtt ccagcctttc attcaagaat atcaggggcc
     5221 attcatcacc tcttttcttt accctaagga ctggcgattt gattttaatc atttttatca
     5281 gtatttaaag caaaacggat ttgtgatcta tcccgggaaa atcagcgatc atgaatgttt
     5341 tcgtatcgga aatattggcg agatctatcc ggaagatatt ttggaactga cagcaattat
     5401 caaagactat aaggagaacg ttaatgatta aaactgtaat ttttgattgg gcaggaacaa
     5461 ctgtggactt tggctgtatg gctcctgtcc acgcttttag aaatgctttt ctagaaaaag
     5521 gaatccaact gactgacaaa gaaatccgcg aaccgatggg aaaattaaaa tgggatcata
     5581 tccaacagct tctttccctc ccttctgtca aaaaacaatg gatagctatt tatggacaac
     5641 tacctcagga aaaagaggtg gaggaattgt atcaacaatt tgaacattat ctgtttaaag
     5701 atttggtcaa gcattctacc ttgaaacccg acacattgga aacgatcgaa cggttgagaa
     5761 acgctgggat caatattggt tccacgactg gatttactgc tcctatgatg acaatcgtag
     5821 cagaaaccgc aaaaaaagaa gggtattcac cagacttcat ggcaacttct gaagacgtcg
     5881 aagggtatgg tcgaccgttt ccttatatga tccataaaaa catgcagtac ttcaaaaaca
     5941 agtcggttga tgaagtaatc aaagtcggag atacggtctc agatatccag gaagggaaaa
     6001 atgccggagt gttgacagta ggtgtaattg aaggtagttc gctaatgggg atttcttatg
     6061 atgagtacca aaaattagag atggggaaaa aagatcgttt aaaacagtct gttgctctta
     6121 gattccaaca agctggcgct gatatttgtg taaacaattt aaaagagtta acagaataca
     6181 ttttgtacgc tgacaagaat ccagaatgac ttttatacaa aatcagaggt cgtgaaaaag
     6241 ctgtactgca tcaagtaata agaacagcca aacaaaaata gctcctcata tttttcgttt
     6301 ggttaggctt attaccgcag atgcaagctt ttgaacaccg tttattcgaa aaaaaaaggg
     6361 agctgtgaca cgacttttgt cacagtcccc ttatttacaa acaaacagtg tttaagagac
     6421 aacctctcca ctaaacggtg ttcattcagc tgactctcgg tatttgctca aaaaacgata
     6481 aaatttgaaa agctattttc tcttttgttt ctctaatact cggatcagaa cgctgatttc
     6541 acattctctt tttcttacat cttatctggt gcatgaagtc ctagcaaacg aaggtcttct
     6601 ttcaataata cagtcactgc ataaactaga gctaagcgtg cttctttttg ttcatcatca
     6661 gctaaaatct tcgtatgagc atagtatttg ttgaatgctt gtgctaattt gattgcatgt
     6721 ttcgcaatca ctgacggttc gtatttttcc ccagcagaca agaccgtttc agggtatttt
     6781 tgaactaatt taaccacttc ccagctggta tcgtcattca atgcatagtc tgctgtttct
     6841 gaagggacaa atcctgcttt ttccaacagg ctgactgcac gagcatgtgt atattgcaca
     6901 taaggtccag tttctccttc aaatcgaaca acttcttcca ggttaaagtc aaatgtattc
     6961 agtcgatcgt ttttcaaatc gtggaaaatg actgccccaa ccccaacttg tttagctaca
     7021 gcatctttgt tttccaaatc agggtttttc tcgctgattt gttctttagc agattggatt
     7081 gcttcattta atacttcttc taataagacg atctttcctt tacgagttga taatttttta
     7141 ccaccttgcg tgatcaaacc aaatggaata tggcgcatat cgtcagacca gtcaaagccc
     7201 atttccttca agacagcttt caactgttta aaatgatagc tttgttcatt ccctactaca
     7261 tatagagatt gtttgaagtc gtaagtacgt ttacggtaca atgcagcagc taagtcacgt
     7321 gtaatgtata gtgtcgcacc gtctgacttc ttaatcaacg ctggattcag gtcataagca
     7381 gataggtcaa cgatctctgc acctttatcc tcgttcagca aatgtttttc ttctaaaagt
     7441 ttgacgattt catccatctt gtcattgtag aaagcttcac cgttcaacga atcgaaacgt
     7501 acttccagca gatcatagat tttattgaat tctttcatcg attcatcacg gaaccattgc
     7561 cataattgaa tagcctcttc atcgccttct tccagacgtt tgaaccaagc acgagcttct
     7621 tcatttaatt ctggttctgt ttcagctact tcatggaatt ggacatataa acgaagcaat
     7681 tcattgattg gttctgcttt aacagcttct tctgtacccc attttttgta agcgacgatc
     7741 agttttccaa actgtgttcc ccagtcacct aaatggttga tgcggattgg ctgataaccg
     7801 attttttcca taatgaaacc gatcgaatta ccgataactg tcgaacgcaa atgtcccatt
     7861 gagattggtt tggcaatatt aggagaagac atgtcgattg gtactgttcc ttgattcccg
     7921 atattgctat ccccataatg ttctttttct ttcactactg tttgcaaaac ttttttgctg
     7981 ataagttctt tattcatgaa aaagttcaag taaggtccga caacttcgat tttttcaaag
     8041 ttggcagaat cgattttttc tgctaaatct gcagcgattt gctgtggcgc ttttcggtaa
     8101 acttttgcta atgaaaaggc tggaaatgca acatctccgt gttcagctga ttttggattt
     8161 tctagtaatt gttcgacttg ttccagtgtt aaatcatctt tgacagcgtc atagacagct
     8221 tttgcgacaa gatctttatt attcatcttt attcctccta aagtgctccc ttgattgtta
     8281 aacaaaaaaa atctctagcg acggcatact tagtatgcca tcactagaga cgagatttcc
     8341 cgcggtacca ctccaattgt cctaaaaaag gaccagctcc atctgatatc ggtagacgac
     8401 cgtctgttta aaacagaatt ctccaagtgc gcttcgttgt atcttcttca ttcggcttcc
     8461 accacttccg aattcgctag ccaagaatga tacaagtact ctcttgttca acaaatctta
     8521 gtcttattaa actttcttga attatatcag aaaaaaaaac gaaaggctag tcgtttctga
     8581 taaaaaaaca atccagatgg cctaaaggag aagaaatacc acctggatag ctataaatca
     8641 tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc cttcttcaga
     8701 agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt ttggttttgc
     8761 ttcaacaaat gcaatagctg cctctacttt cggaagcatg cttcctggag caaattgctt
     8821 ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct tttgatctgg
     8881 tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca aatccgcatc
     8941 gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt ctcgtccttc
     9001 taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga ctgtgataac
     9061 tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg gtttagggga
     9121 agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag tggagtcagc
     9181 attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt tacttggatt
     9241 tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa ccacatcttt
     9301 atcgatccct ttttccttca gtacttcgcc catggcattt tgtaaccagt aaccgataga
     9361 tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct cagaatcagc
     9421 agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg agatgatcaa
     9481 ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta acgcttcttg
     9541 ttgggctttt gcacttgcat ccgtagacaa gattgcatta ccccctaaag cgacaactac
     9601 ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc tagataacaa
     9661 aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt gagatgaatg
     9721 tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca tgatcgcttt
     9781 gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc tgcggaagac
     9841 ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt caccgtaaac
     9901 agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat ctgtgttgtg
     9961 tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga tacgttcttc
    10021 gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt ctgctccgtc
    10081 tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt cagcaaagcc
    10141 ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa cgatacgcat
    10201 attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt ttcgaccatc
    10261 tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga tagttaaaaa
    10321 gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa ctggaacacc
    10381 agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt cgattccatc
    10441 aaacatacga cctagtactt tagctgtatc ttctgtggat tcttttttac ctaattggat
    10501 atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag tagtaaatgc
    10561 agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc cttctagata
    10621 gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat cgatcaaata
    10681 ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt ggaatactga
    10741 ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa gaggaaatcc
    10801 cgacggcagc tgacagtaag accgccgtca ggaaacaaat tacttcaagt cttcacggac
    10861 aagtggttgg ctcatgcagc gtggaccacc acggcctcgt gacaattcac ttgaattgat
    10921 ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat ttcggttata
    10981 agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc attgttctcg
    11041 aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat ctaatccaag
    11101 agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat catcaccatc
    11161 tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg gatggatagt
    11221 gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg cacggttgtt
    11281 tggaattttg attgctaaca ctttttcaaa tcctgaattt tttgcgaata ggtggcgtgc
    11341 taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta ctactttatc
    11401 gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat ccaaccatac
    11461 ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg taaacatcga
    11521 ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc ccattgatgc
    11581 ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg gatagtcatc
    11641 gtctgctgac agatcgtata gactgctaga acgtacttca atatcttttg tacgaacccc
    11701 agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact catgaagacc
    11761 ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca agaattgttc
    11821 tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa gagtctcgac
    11881 gccttcattt tgcaatgtct ttgcaaagtt gtcatgttct tcttgagcaa tcggaagata
    11941 tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt ccacttcttg
    12001 tccaggcctt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa cgtgaatagg
    12061 tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat cctccttttg
    12121 taatcacttt cataattttg tctcattcaa aatgagacat tcctcacatt aatattcgat
    12181 aattaaataa ttaaacgttt ttctattttt aagcgtaatt tattcatttt ttattctgta
    12241 tacactttca aaaaaatatt tattcattga aaaaaatttt ttgaataaat taaaaaaata
    12301 aattatatta gcgttgttag cgtttactat atgtattatt gttgtttttt atacacaaat
    12361 cattcgcttc aaaacaaatt attcaatacc attgtatatt ttttacataa taacaaatta
    12421 tatttg
//
LOCUS       DASJTC010000080        12406 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.081, whole genome shotgun sequence.
ACCESSION   DASJTC010000080 DASJTC010000000
VERSION     DASJTC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.081"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(6..812)
                     /locus_tag="U4B19_002109"
     CDS             complement(6..812)
                     /locus_tag="U4B19_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077836.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            950..2464
                     /locus_tag="U4B19_002110"
     CDS             950..2464
                     /locus_tag="U4B19_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEN2077837.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(2867..3850)
                     /locus_tag="U4B19_002111"
     CDS             complement(2867..3850)
                     /locus_tag="U4B19_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEN2077838.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(3855..4502)
                     /locus_tag="U4B19_002112"
     CDS             complement(3855..4502)
                     /locus_tag="U4B19_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2077839.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(4587..5219)
                     /locus_tag="U4B19_002113"
     CDS             complement(4587..5219)
                     /locus_tag="U4B19_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2077840.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(5235..5786)
                     /gene="coaC"
                     /locus_tag="U4B19_002114"
     CDS             complement(5235..5786)
                     /gene="coaC"
                     /locus_tag="U4B19_002114"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEN2077841.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(5783..6520)
                     /locus_tag="U4B19_002115"
     CDS             complement(5783..6520)
                     /locus_tag="U4B19_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEN2077842.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(6517..7506)
                     /locus_tag="U4B19_002116"
     CDS             complement(6517..7506)
                     /locus_tag="U4B19_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN2077843.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            7764..8306
                     /locus_tag="U4B19_002117"
     CDS             7764..8306
                     /locus_tag="U4B19_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2077844.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            8323..9408
                     /locus_tag="U4B19_002118"
     CDS             8323..9408
                     /locus_tag="U4B19_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077845.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            9420..10226
                     /locus_tag="U4B19_002119"
     CDS             9420..10226
                     /locus_tag="U4B19_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2077846.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            10227..11054
                     /locus_tag="U4B19_002120"
     CDS             10227..11054
                     /locus_tag="U4B19_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2077847.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            11051..12124
                     /locus_tag="U4B19_002121"
     CDS             11051..12124
                     /locus_tag="U4B19_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2077848.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
BASE COUNT         3875 a         2243 c         2563 g         3725 t
ORIGIN      
        1 atgttttatc gtttgatttt tttaggaata aaattcttga tgaacgtaaa tttttggtaa
       61 tcgtttcgac tatagaggta accaaataca ctgaaaacaa ctgctccaat aaaattaaca
      121 aataaatcct tcatagtatc catgattccc acgtctaagt aaccattttg gatgattgtt
      181 ttatctgttt taccatctgc attttgactt tcaatgattg tccgagtgat atctggaaca
      241 tgatagacag tgtttccgtc tttacttaac tccacactat tgatttgctg gatgatcgta
      301 tctttttgca tatcgaattg cagtaattta tctgctgaaa attccacaaa ctcccacata
      361 acaccaactg tcatcgaaaa acagaaggaa acaactgcta gtaataaagg agtcaagttc
      421 atggtttttg tatttttatt gagcaaataa acaagggaaa agccgacacc cccagctaaa
      481 aatccgctga atgcatgtag aaaagtatcc cacattgaaa aatagccata aaaatcactt
      541 aactctccta aaactgttga actaaaaata aaagataaca aaatgatttc tagcatattt
      601 ggaatctgta tctttaatat cttttccaga aaataaggga tttcaaataa aattaatgtc
      661 agtaataaaa gcattgtatt tccccagtgt tggttccaga cctgatccac tagcataata
      721 acgacgatga tacgtaataa aatatagatt ccgtttgtga ttctacctgc agtttttgta
      781 ctgttcgtaa aaaaggtttt caaatgattc ataaaataac tccttcatgt atatccttaa
      841 ttatactaag aagcgtgtgt ttaccaaagc tatatgactt aaagaaaaac agagttgata
      901 ttgtgaaaaa aatcacttaa tatagtataa ggagaaaagg gggatacaaa tgaaaaaatt
      961 agttgctggt ttattaacag ccgttgcact tcttgctatg agcgcatgcg gcgcagatca
     1021 gaaaaataca gcgcagacaa gttctagtac gaaggaatct gccgaagtat catcaggcgc
     1081 ttctgaacaa gtatatacag atccaaaaga aatgaaagaa tcttatgatg tcgtgattat
     1141 tggagctggt ggtgcaggga tggcagcagc tatcgaagca aaagatgctg ggttgaatcc
     1201 agtaattttg gaaaagatgc ctgttgccgg aggaaacaca tctaaatcat caagcggtat
     1261 gaatgcttca gaaacaaaat ttcaaaaaga agaaggaatc aaagactcga atgatgcctt
     1321 ctatgaagaa acgctaaaag gtggtggagg aacgaatgat aaagaaatgc ttcgatactt
     1381 tgtcgatcac tctgctgatg cggtcgattg gctagatcaa aacggtatca aactggacaa
     1441 cttaacgatc actggaggga tgagtgaaaa gaggacacat cgtccgtctg atggctcggc
     1501 tattggtgga tacttagtgg aggggttatt gaataacgtc catgagcggg aaatccctat
     1561 ttttgtcaac gcagatgtga cagacattaa gaaaaatggg gaaacggtca atgaagtgac
     1621 tgtacatgtt caaggagaag aaccaaaaga ggtcaccgga aaagcaatcg tagttaccac
     1681 aggcggtttt ggtgccagca aagaatttat tgaaaaatat cgtcccgatt tagctgatta
     1741 tattacgaca aatcaagaag gatcaactgg agatggtatc accatgattg aaaaattagg
     1801 tggacaaacc gttgatatgg ataaaataca aattcatcca actgtgcagc aggatgaggg
     1861 ggtcttgatt ggtgaggttg ttcgcggaga aggagcaatc ttggtcgatc aagaggggaa
     1921 aagatttgtc aatgaaatgg atacaaggga taaagtatct gcagcaatta cagcattgcc
     1981 tgaaaaatca gcttatttga tttttgacca aggcgtgcgt gatcgtgcca cagctattga
     2041 attttatgac caaaaaggat atgtcacaac tggaaagaca atggaagaat tagcagaaaa
     2101 aatcgattta ccaaaagaaa cactagcaaa aacagttgat ggatggaata cggatgttgc
     2161 ggagaaaaaa gatgctcaat tcaaccgtgc cacagccatg gaacatgact tatcgtctcc
     2221 gaattattat gcgatcaaaa tcgcaccagg aatccattac acgatgggcg gtgtgaaaat
     2281 caatacgaac actgaagtat tagataaaga aaaccagcca attagggggt tatatgctgc
     2341 tggtgaagtc acaggtggct tacatggaaa caaccggatc ggtggaaatt cagttgcgga
     2401 gatcattatt tttgggcgac aagcgggcat acaagcagct cgatttgctt cggaaaatga
     2461 atgaaataga acgtaaataa aaagaggttg tgaaatcagc gttttgagct aataccgagg
     2521 gtcagctgat tgaacactgt ttagtgaaga ggttgtgaaa aagctgccct gcatcaagta
     2581 ataagaacag ccaagtaaaa acagcttctt atgtttttaa cttggttgga cttattaccg
     2641 cagatgcaag cttttgaaca ccgtttattc ggaaaaagag cgtgggacaa aagtaaagaa
     2701 cacttttgtc tcacgctcta aacacgtata aacggcggaa gcagaagaaa ttccttcgga
     2761 aataagctga aattcacaaa aatttgaaaa gcaattttcg tgaatttctt cttatttctt
     2821 ggagttaaac gcttctgtcc cagcctcttt tttgatcagc atgattttat tcagttatct
     2881 tcaaaatgat ttttccgatg tttttgtttg cttccatata ttcatgcgct tgtttgactg
     2941 cgctgaattg gaatgtacgg tcaatgattg gccgaatctt gccattttct agaaaaggcc
     3001 cagctttttg cataaaagtt tgtgcgagct ctgctttata ctgatcactt ctaggtgtca
     3061 ataatgtacc aagcagttgg acacgtttcc caatcaatac accaaggtct atctcaggca
     3121 cgatggtccc acctagcata ccaatcaaga cccaacgtcc atcaacggct atagaagcta
     3181 aattcttttc ccagtaagaa gcgccgatga aatccaaaat aacatcgact ccccgacctt
     3241 cagtgatttt tcgtacctct tcttgaaagt cttgtttttt atagttgatc aattcatctg
     3301 caccaagttc tttacaaaag gcgagttttt cttctgatcc tgcagtaacg atgattttcg
     3361 cgtgagaaag ctgtctagct aattggatcg ctgctgttcc cacaccactt gctcctgcat
     3421 ggatcaagac agtttcgcct tcttgtaatt ttccaagcca gtaaagtgtt tgataagcag
     3481 tcaaaaaaac ttctgagatt ccagcagcag aaacatagtc tagcgtatca gggagaagga
     3541 ttgcacgatc ggcaggcatc acagcatatt cggcataacc acctagattg actaatccgt
     3601 atacttttgt cccaggagta aaagtggaga tatcactttt attctcaact acctctccag
     3661 ctacttcgac tcccaaaata gggtagggag gtttcaaaga gactgattct cttttcataa
     3721 tatctgttcg attgacagct gctgtatgga ctttgatcag taattcgcct tgttttggtt
     3781 taggaacagg cacttcaatt tgttctaata catctacaga accaggatgc ttgattgaaa
     3841 tggcacgcat ggatctattc ctcctttgcc cattcttgga atgctgtatt caactctttg
     3901 ctaccttcag aaagtactag agtcgtacga gaaatatccg tttcttccac tagagcggca
     3961 agtacagcag caactgtaga gcgagtgatc gtttttgatg ccttctcatt aggcttagtt
     4021 aattgaatgg aagtaacttc ttcatcgtta gtcaatgtga ctggttgaag aatgacaaaa
     4081 tctaagttag ttcgtttcag ccattcatct gcataatgtt tagtaatata ataatctgtc
     4141 atggactcag gccattttgc tggttcatct gcataaacag cactgaccat cagatagcga
     4201 gagacatgcg cattttcagc tgcgatcact gttttgattg cgccatccaa gtctacttgc
     4261 agaagattct ttccttgaga gccggcagca aaaatcaata catcgatgcc tttgaatgct
     4321 tcggtcattt tttccacgga ccaagttaaa tcaaaagaga catacgaaac attctctttt
     4381 tttactaatg tttgggaagc aacatctctc acccctgcaa aaatttcatg ggaacttgga
     4441 gctaaatctt ttatcaggtg tcggccaatt tgtccgtttg cacctacaac aaaaattttc
     4501 atttggatca tcctttcgac gtcaaatctt tccattcaat agtaacacca aaagagaaaa
     4561 aaatcagaga tcggctacga tgactatcag ttagtaagag aagggtgctt gaagtatctt
     4621 aacaagacat tgcatactgc tgcagtcgca agacctgcga cgattccttc aggtatacca
     4681 cttgtaaaaa taaccgctag aatagctgaa taaacagcat ttaaatttgt tccgatgact
     4741 ttggcataat cttgctggaa taaaaagtag atcatattca tcactaaaat cgtattggtc
     4801 aatgaaccgg aaagtccagc taagaataaa gaaacggaat ctgctttttg tttcattagt
     4861 ttgcgcagac ctttataaac gaaataagga accaccccaa tcaagatgcg aggaataaac
     4921 gcgatcagca aggctttcca gcttccatgt tctgttccga tgactgggat aaacggagaa
     4981 aagacaaaag acaaaggtgt ttggataact gtgctacgga tcatgctgat cagtccaaat
     5041 gtaccgccaa gaaaagcacc tagtcttggt cctaaaacga tcgaagcaat gattacagga
     5101 atatgcatgg ttgttgcatt gattggaccg atagggataa aacctaaagg cgtaacagct
     5161 aataaaatca aaatagctaa aaacatcgca gttaatgtaa agttttttgt atttttcata
     5221 agtactcctt ttttctaagc atttctttca ttaagagtca gcatgacctg ttcgatgatc
     5281 gtctgaatat cagctaaggc accttttcca aaatctccac aagctaaaag agattctctt
     5341 ggttcaattt cctgatagcc gacagatctc aatgtttcca gattacgctg agtgatcggg
     5401 ttttgataca tattcgtgtt catggcaggg gcaatcaatt ttggtgttcc cactggaagc
     5461 gccattgcga ctgttgaaag aagatcgtct gcaatgccgt ttgctagctt gccgataatg
     5521 tttgctgtag caggcgcgat gagaaacaag tcagcttgtt ttgctagttc gatatgattg
     5581 attacctgcg gatttgattc ttgcatgacg tctgtatgga ctgcgcgctt cgacagagat
     5641 tgcaaggtta atggcgtgat aaaatgtgtg ctgcttttag acataatcac atctacctgg
     5701 gcacctaatt tgacgaattg acttgtaaga tcagcgcttt tgtaagcaga aatacttcca
     5761 gtaacgccta gtaagatctt tttcattttg attcctccag tctaattttc tcagtaatca
     5821 atgcagcaat ctcagattta ctttgagctt cttccactgt tccatcttta gataagagat
     5881 agccgtggtg tgtctcgcca tggatttccg tcaaatcatt tgcgaaaata aaatcagttt
     5941 tgttatttat cagactattt tttgcgactg ccagaagaga ttcctttggt acatctacta
     6001 atagtttgaa accaatgatt tttgcttttg gctgccattc acgtagataa gaaatgattt
     6061 ttggtgtttt cttcaaaacg agcacaagat gctcggtgtt agaagagatc tttgaaacag
     6121 tggtatctgt ctgttcattt tccgcaaacc aattgtccag ttcttcttgc gttgaagaag
     6181 tgggtaggtt ttttgccagc tgttcttctg aaaaactaaa agcaggagta aaatcactga
     6241 ctgccatact gtggataatc gcatcgtagt gttctttttt cgttagttct tctaattgta
     6301 aaaataagtc cagtgcagat tcgatcgtat aaatcgttat attggagtgt cttttcggtt
     6361 tcagtgcttg tttcgtagtc acgtaatcga ttgtggtatc cggattggca gctaaataat
     6421 cagctaatgc ttgccctaat cgtcctgtcg aatgatttgt aatagcacgt acttgatcga
     6481 ttttttcagt cgttcctcca gctgtgatta gaatcttcat ttatggtcac ctacatttac
     6541 tagtgttctt ccttggtgtg ttccagcaag tagtttagat gctgtttcag gcaattgatc
     6601 cagagtaatc tcttggacga ccagttcatc tgcaatctgc aaatccgcca tctgttgcca
     6661 taaagaaaga cgttgatcta ttggtacatt gacagaatca attccaatca actggatgtt
     6721 tcgcagaata aaaggcaaga cagtcgcgtt tagtttgatt cccccagcat tgccacataa
     6781 agctactgca ccgttataag agatcaatgg aagaagactg cttagctgct cgccacctac
     6841 tgtgtcgatg acatagtcga tttgctgttt tcctaaagct tttgttgtct ctggcagaaa
     6901 ttcatcaggt cgtacgactt gcaaagcacc tagacttttt agccatgtga cagactcttc
     6961 ttttctggaa aaggcaatga ttgactggaa tcctagttta tgtaacaggg ctatcgctgt
     7021 actgcctaca ccgccagaag caccagtaac gacaactttt ttatcggagg tcataccttg
     7081 acggattaaa gcgttcacac acaacatagc agtaaaccct gctgtaccaa ggatcatcgc
     7141 ttgacgcagc gtcatatttt ttggtagagg aactaaccag tctcctggta ctttttgata
     7201 ttggctataa ccgcctggat gggtgacgcc aagcccaaaa ccagtagcaa gtacaagatc
     7261 ttcttttgaa aaacgtggat tgttactttg gacgacagtt ccggataaat caatccctgg
     7321 agtcatcgga tatgttcgta tcacctgacc agactcgctc attgccaatg catctttata
     7381 attgacatca gaataggcaa tttttaccaa gacttcattt tcagccaact cagtaaatgg
     7441 cagatcactg attgctgtat ggaaaacagg ttcctttatt acctgaaaag atcgaaaagc
     7501 gttcattcgt gagacactcc ttttaaaatt ttcttttatc ataccataaa gcctactctt
     7561 ttattcttgt tcttccgtaa atttagacaa aaattaggtg aaaaataaag aatcaaacaa
     7621 ctagaattta ttccaaaaga taaagaaata ttcagcaaaa aaaattttac aaagggttgc
     7681 ctttttcaat aaatttcgta tactaatacg agtggtagta aacaggaagt ttactattag
     7741 aaaacttcga ttcggaggaa attatggaaa ttggtgagaa gttaagaaat ttgcgcatcc
     7801 aaaagaattt gacacaagaa gaactaggcg aacgcacaga tttgtcaaaa gggtatattt
     7861 cccagttgga acgagattta agttcaccat caatggaaac attcttttct attcttgaag
     7921 tcttaggtgt gactccagaa gaattttttc atcaagaagc agcgaatctg caagtggttt
     7981 attcaaaaga agatcatacg gtttatttcg atgaagaaaa cgggtatgaa ttggaatggc
     8041 tcgtggctga ttcgaatgaa aaagaaatgg agcctgtctt gctgactttt gaaggtgcag
     8101 gagaatataa gacgtttgaa ccttcattat ctgaaacgtt tatttttgta ttagaagggg
     8161 aattagagtt aagcttagga gagacggtct atgtggcaaa agcaggacag tcgatctatt
     8221 atcaagcgac aaaacaacat caattaagga atcatctcaa aaaacgaaca aaagtattga
     8281 ttgttgcaac gcaatcatat ttgtaatata gggggtaaaa acgtggggaa accaatcatt
     8341 tcatttgatc aagtagtgaa acgctatgat gatgaaacag tattaaaaaa agtaagtttt
     8401 gagatcgaac aaggaaaatt ttatacactg cttggtcctt ccggctgtgg taaaacaacg
     8461 atcctacgta ttatagctgg attcaccgaa gctacagaag gggacatcta ttttgaaggg
     8521 aaacggttga atgatttgcc agctaataag cgacaagtaa atacagtctt ccaagattat
     8581 gcactttttc ctcatatgaa tgtttttgat aacgttgctt ttggattgaa gatcaagaaa
     8641 atgtccaaat cagatatcga aaagaaggta aaagatgctt tgcgtatggt ccagcttcct
     8701 ggatacgaaa aacgggagat tagtgaaatg tctggtggtc aaagacagcg tgtagcgatt
     8761 gcacgagcaa ttgtaaatga accgaaagta ttgctattag atgaaccgtt atcggcgctt
     8821 gatttgaaac tacgtacaga tatgcagtat gaactgcggg atctgcaaca acgattagga
     8881 atcacgttta tttttgttac tcatgaccaa gaagaagcgt tggcaatgag tgatgagatt
     8941 tttgtcatga ataaagggaa aatcgtccaa agtggtacac ccgtagatat ttatgatgag
     9001 ccaatcaatc atttcgttgc tgattttgtt ggggagagca atatcgtgga cggtgtaatg
     9061 atcgaagata atctagttga gtttgttggt aagcaatttg aatgtgtcga tggcgggatg
     9121 cgtctaaatg aaccagttga agttgtttta cgcccagagg atttgacgat cacgacacca
     9181 gataaaggaa aactagtcgt aacggtcgat actcagctgt ttcgtggtgt ccattatgaa
     9241 atcatctgtt atgatgaaca gcaaaatgaa tggatggtcc attcaacgaa aaaagctaaa
     9301 gaaggaagca aagtaggatt ggcctttgag ccagaagata tccatgtcat gcgtttcaac
     9361 gagtctgaag aagaattcga tgcacgtctt gacagctacg aagaataggg gggacaagaa
     9421 tgaaaacaat gcgcaggatc tattccgtcc cttatatttt gtggctcggt ttattcgtga
     9481 tcgcgcctgt attaatgatc atttatcaat ctttttttga tatgaatggg caattcacgc
     9541 tgaataatta tctatcttat tttacgtcgg ggacatcgct ttctatgacg atcaattctg
     9601 tatggtatgc atttttgatc acgtttttca cgctgatcat cagttacccg actgcttatt
     9661 ttttaacaaa actgaagcac aagcaattat ggctgatgct gattattttg cctacatggg
     9721 tgaatctgct gcttaaagct tacgcattta ttgggatttt cagtgtgcac ggcagcgtga
     9781 atcatttctt tgattttatc gggatcgggc cgaaacagat attattcaca gacttcagtt
     9841 ttctgttcgt ggcaacgtat atcgagattc catttatgat tatgccaatt ttcaatgcat
     9901 tggaagagat gaatccttct ttgatcagtg ctagtagaga tttaggcgcg aatagctttg
     9961 agaccttccg acgagtgatt tttccgttat cattaaatgg tgtgaaaagt ggtgtccaag
    10021 cagtatttat tccttctctt tctttattca tgctgactcg tctgattggc ggaaatcggg
    10081 tgatcacttt aggaacggct atcgaacagc atttcttggt tacacaaaac tgggggatgg
    10141 gttctacaat cggtgtgatt ttaatcattg ctatgttcat cgtgatgctc ctgacaggcg
    10201 agaagaaaaa aggaggtcgc tcataaatga aaaaatttaa atggtcgtat ctttatttag
    10261 ggatcgtctt tttattccta tatgcaccga tcttctatct gattttttat tccttcaatg
    10321 aaggaggaac gatgaacagc tttacaggat ttacttggga aaattatgaa gctgtttttg
    10381 aagatacacg tttgatcatt atcgtattga atacgttcat gctggctttc ttatctgcat
    10441 tgatcgccac gatcatcggg acatttgggg ctatgggaat ctattacacg aaaaaacgac
    10501 gtgcacggac agcgttgttg agcatgaata acatcttatt agtctctcct gatgttatta
    10561 tcggtgctag tttcttgatt ttctttacac tactaggatt cggattaggt tttcagagtg
    10621 tattgctttc ccatattgct ttcagcattc caatcgtcgt cttgatggtc ttgccaaaat
    10681 tgcaggaaat gaacgattca atggtcgatg cggctagaga ccttggtgct aataatttcc
    10741 aagtagtcaa aaatatcatt ttgccttatc tttctccagg aatcatagct ggatatttta
    10801 tggcatttac ctattctctt gatgattttg cggttacatt tttcgtcaca ggaaatggct
    10861 ttacgacgct ctcagtagag atctattctc gtgcaagaca aggaatcagt ttagaaatca
    10921 atgcgctaag tgcgttagtt ttcttgttct ccatgatatt ggtcattgga tactacttta
    10981 ttagtaaaga aaaccgtcca aaacggctga agaaaaacag aagagtacgc gagggggtgg
    11041 cacggctgcg atgaaaaaat tacaatctct gtttttaggt attctcatca ttattgcggt
    11101 gctgctgctt ggtgttcgtc aacttgaaaa aacaagtggc atgagtggag caaaggtact
    11161 taccatctat aactggggag attatatcga tcctagtttg atcacgaaat ttgaaaaaga
    11221 atatggatac aaagtgaatt atgaaacatt tgattctaat gaagcgatgt tcacaaaaat
    11281 ccagcaaggt gggacaaact acgacatcgc tataccaagt gaatatatga tccaaaaaat
    11341 gatcaaagaa aaactagtac ttccattaga tcattcgaag atcaaaggat taaataatat
    11401 tgatccacga tttttagact tggattttga tccagataat cagtattcga ttccatattt
    11461 ttggggaact ttagggatcg tatataacga taaattcttt gatgcaaatc aaattaagca
    11521 ttgggatgat ttgtggaagc cggaattaaa agacagtttg atgctgattg acggtgcacg
    11581 agaagttatg gggctttctt tgaatagttt aggctattct ttgaatagta agaatatggc
    11641 acagctgaca gaagcagcaa ataaattgaa tcatttaaca ccaaatgtca aagcaatcgt
    11701 ggcagatgag atcaaaatgt atatgatcaa tgaagaagcc tccgtggcag tgacattttc
    11761 tggcgaagct gccgatatga tgtgggaaaa tgaacattta cattatgtga tcccaccaga
    11821 aggatccaat ctttggtttg ataatatcgt tatgccaaaa accgtgaaaa ataaagaagg
    11881 tgcctatgat ttcatcaact ttatgctaga accggaaaat gcggcacaaa atgcagagta
    11941 tattggttat tctacaccaa atgaagaagc aaaagcgtta ttgccaaaag acatttcgtc
    12001 ggacgaacaa ttctatccat ctgatgacac gatctcccat cttgaagtct acaaagatct
    12061 aggatcgaaa tacctaggca tctataatga cctattctta gaattcaaga tgtataggag
    12121 ataatggcaa aactcagtaa gaatggggac ttatcaacgt tctaaagagc taaaaatcgt
    12181 cctttttcta tctgagtttt ccaatcttct accaaaagcg gatgaaacgc cgcttttttt
    12241 gtgttatttt attttttcta ccacgttttt ctaccagaga aaagtggaca aaaccattgt
    12301 aaaatagggc ccgagacggg ctagtaaatc taagattgaa aaagttacag aaggataaag
    12361 taattggaaa ctatgcaaat atacttaacc cttctgtaat ctatta
//
LOCUS       DASJTC010000081        12173 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.082, whole genome shotgun sequence.
ACCESSION   DASJTC010000081 DASJTC010000000
VERSION     DASJTC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.082"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..610
                     /locus_tag="U4B19_002122"
     CDS             <1..610
                     /locus_tag="U4B19_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2077849.1"
                     /translation="KRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWIQLIL
                     DEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSKITVF
                     PKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAYTIQN
                     ATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            723..1001
                     /locus_tag="U4B19_002123"
     CDS             723..1001
                     /locus_tag="U4B19_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEN2077850.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            1119..1355
                     /gene="secG"
                     /locus_tag="U4B19_002124"
     CDS             1119..1355
                     /gene="secG"
                     /locus_tag="U4B19_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEN2077851.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            1458..2213
                     /locus_tag="U4B19_002125"
     CDS             1458..2213
                     /locus_tag="U4B19_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2077852.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            2216..4573
                     /gene="rnr"
                     /locus_tag="U4B19_002126"
     CDS             2216..4573
                     /gene="rnr"
                     /locus_tag="U4B19_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEN2077853.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            4595..5059
                     /gene="smpB"
                     /locus_tag="U4B19_002127"
     CDS             4595..5059
                     /gene="smpB"
                     /locus_tag="U4B19_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEN2077854.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            5361..6080
                     /gene="atpB"
                     /locus_tag="U4B19_002128"
     CDS             5361..6080
                     /gene="atpB"
                     /locus_tag="U4B19_002128"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEN2077855.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            6133..6348
                     /gene="atpE"
                     /locus_tag="U4B19_002129"
     CDS             6133..6348
                     /gene="atpE"
                     /locus_tag="U4B19_002129"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEN2077856.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            6439..6963
                     /gene="atpF"
                     /locus_tag="U4B19_002130"
     CDS             6439..6963
                     /gene="atpF"
                     /locus_tag="U4B19_002130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEN2077857.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            6950..7492
                     /locus_tag="U4B19_002131"
     CDS             6950..7492
                     /locus_tag="U4B19_002131"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEN2077858.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            7515..9071
                     /locus_tag="U4B19_002132"
     CDS             7515..9071
                     /locus_tag="U4B19_002132"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEN2077859.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            9085..9987
                     /locus_tag="U4B19_002133"
     CDS             9085..9987
                     /locus_tag="U4B19_002133"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEN2077860.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            10010..11416
                     /gene="atpD"
                     /locus_tag="U4B19_002134"
     CDS             10010..11416
                     /gene="atpD"
                     /locus_tag="U4B19_002134"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEN2077861.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            11430..11852
                     /locus_tag="U4B19_002135"
     CDS             11430..11852
                     /locus_tag="U4B19_002135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEN2077862.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4046 a         2159 c         2687 g         3281 t
ORIGIN      
        1 taaaagatat gcagagaaag gattcaacgt tcttgtacct gatttacgtg cgcacggaga
       61 aagtgaagga gaaatcatcg gaatgggatg gctagatcgg ctagatttaa ttgcctggat
      121 ccaactcatc ttagatgaac agccgaatgc gtccattatt ttacacggcg gatcaatggg
      181 agcttctacg atcatgatgg caagtggtga aaaacttcca tcagcggtca aaggatttat
      241 tttagacagc ggatatgttt ctgtttatgc agaattccga tatatgttaa gtaaaatcac
      301 tgtctttcct aaaaaaatgg tcatgagata tgcgaatcat tatgcccaga aatacgccgg
      361 ttactcctta aagcaagcat cagctacgag acagcttggc agcaaccatc tgcctttatt
      421 agtcatccat ggtgaaagag atcattttgt ccctacagaa gcagcgtaca cgattcaaaa
      481 tgctacggca ggggataaag cactgctgct tgttccagaa gcagaacact tggaagcatc
      541 gatgaaagat ccgaaaacct attggacagt gattttttca tttattgaac aaagaataac
      601 gatggattaa ttttctcttc ttttttgtaa gtaattcgag gataaataat tgtttataga
      661 tctggagact gctaaaataa ggtaggaata aaaaaatatc agttatttga ggagtgcggc
      721 atatggaaat caaagaagaa aaaaatcgtc ttgctttatt caatgatgag caacaagaga
      781 ttggagaaat gacatggtca gatgcgggcc cagacatcat gatcattgac catacatttg
      841 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
      901 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
      961 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     1021 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     1081 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     1141 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     1201 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     1261 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     1321 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     1381 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     1441 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     1501 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     1561 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     1621 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     1681 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     1741 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     1801 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     1861 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     1921 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     1981 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     2041 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     2101 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     2161 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     2221 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     2281 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     2341 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     2401 agtgaaactt cctttaaaac caatcttgat cgaaggaaca tttcgagcaa atgaacgagg
     2461 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     2521 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     2581 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     2641 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     2701 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     2761 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     2821 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     2881 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     2941 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     3001 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     3061 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     3121 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     3181 tgtatatttg actgatcgag tgattccgat gatcccgcaa cgtttatcga atggtatctg
     3241 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     3301 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     3361 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     3421 tcagctcgta cctatgttcc aagaaatggg tgaattgcat cgaatccttg aaacgatgcg
     3481 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     3541 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     3601 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     3661 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     3721 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     3781 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     3841 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     3901 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     3961 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     4021 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     4081 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     4141 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     4201 aggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     4261 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacaaaaag tgcgcgtaaa
     4321 agtagtcaaa gctgatccag atgaacgagc agtggatttt gaactcgttt ccgcagaaga
     4381 ggtcactgcg ttggaacgtc caaaagcacg caatggaaaa tctggaaaac gagaaggaaa
     4441 acaagcaggc aaacgctcag aaaaaaatgg caagaagaac aaacgatata gtgatagtaa
     4501 gcaaacgttc ggtaagaaaa aagggaaaaa gccattttac aaagatgtca aaaagaagaa
     4561 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaactaat
     4621 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     4681 ggtcttgcaa ggaacggaga tcaagtcgat acgaaacagc cggatcaatc tgaaagatgg
     4741 atttattcgc gtccgcaacg gggaagcttt cttgcataat gttcatatca gtccttatga
     4801 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaagaa
     4861 acaaatcgtt cgtcttgaaa acgaattgaa aaacactgga atcactgttg ttcctttaaa
     4921 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcaaaag ggaaaaaatc
     4981 gtatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     5041 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     5101 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcac aggttttttc
     5161 ttagatcagg agcaccgtgc atggggaaaa attcgcttcg tttttgactg aaataaaaaa
     5221 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     5281 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     5341 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gtttcacatt ggaccaattt
     5401 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     5461 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     5521 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     5581 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacataggtt
     5641 tagtaacaaa aatcgtctta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     5701 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     5761 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     5821 ttccgatgaa actgattgaa gaatttacaa atttattaac acttgcttta cgtctttatg
     5881 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     5941 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     6001 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     6061 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     6121 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     6181 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     6241 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     6301 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     6361 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     6421 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     6481 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     6541 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     6601 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     6661 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     6721 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     6781 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     6841 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     6901 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     6961 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     7021 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     7081 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     7141 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     7201 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     7261 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     7321 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     7381 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     7441 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     7501 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     7561 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     7621 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     7681 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     7741 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
     7801 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
     7861 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
     7921 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
     7981 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
     8041 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
     8101 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
     8161 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
     8221 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
     8281 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
     8341 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
     8401 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
     8461 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
     8521 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
     8581 cggttgatgc cggactatct gtatctcgtg tagggggttc tgcgcaaatc aaagcaatga
     8641 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
     8701 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
     8761 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
     8821 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
     8881 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
     8941 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
     9001 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
     9061 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
     9121 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
     9181 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
     9241 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
     9301 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
     9361 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
     9421 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
     9481 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
     9541 tctgatcagc caagctttga tgaagtccgc aaaatcgtaa atatggcaac aactatgttc
     9601 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
     9661 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
     9721 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
     9781 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
     9841 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
     9901 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
     9961 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    10021 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    10081 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    10141 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    10201 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    10261 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    10321 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    10381 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    10441 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    10501 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    10561 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    10621 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    10681 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    10741 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    10801 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    10861 attacaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    10921 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    10981 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    11041 ggcttcttct tcaagtgctt tagcacctga aatcgtgggg gaagaacatt ataaagtagc
    11101 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    11161 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    11221 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    11281 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    11341 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    11401 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    11461 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    11521 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    11581 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    11641 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    11701 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    11761 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    11821 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    11881 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    11941 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    12001 tggaacaccg tatatcagag gttgtaaaat cagcgttttg tcctgagtat tagagaaaca
    12061 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    12121 tgaacaccgt ttatttgggc tctttgtcaa atgatgttgg taaagagaaa tct
//
LOCUS       DASJTC010000082        11945 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.083, whole genome shotgun sequence.
ACCESSION   DASJTC010000082 DASJTC010000000
VERSION     DASJTC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.083"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            578..1183
                     /locus_tag="U4B19_002136"
     CDS             578..1183
                     /locus_tag="U4B19_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEN2077863.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="U4B19_002137"
     CDS             1199..1771
                     /locus_tag="U4B19_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2077864.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="U4B19_002138"
     CDS             complement(2023..2280)
                     /locus_tag="U4B19_002138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077865.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="U4B19_002139"
     CDS             complement(2406..3221)
                     /locus_tag="U4B19_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077866.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="U4B19_002140"
     CDS             3738..5063
                     /locus_tag="U4B19_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN2077867.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="U4B19_002141"
     CDS             5056..5406
                     /locus_tag="U4B19_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077868.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5388..5573
                     /locus_tag="U4B19_002142"
     CDS             5388..5573
                     /locus_tag="U4B19_002142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077869.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6477..6752)
                     /locus_tag="U4B19_002143"
     CDS             complement(6477..6752)
                     /locus_tag="U4B19_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEN2077870.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6724..7677)
                     /locus_tag="U4B19_002144"
     CDS             complement(6724..7677)
                     /locus_tag="U4B19_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2077871.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8289..9782
                     /locus_tag="U4B19_002145"
     CDS             8289..9782
                     /locus_tag="U4B19_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEN2077872.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9917..10213
                     /locus_tag="U4B19_002146"
     CDS             9917..10213
                     /locus_tag="U4B19_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN2077873.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10314..11165
                     /locus_tag="U4B19_002147"
     CDS             10314..11165
                     /locus_tag="U4B19_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077874.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11186..11469
                     /locus_tag="U4B19_002148"
                     /pseudo
     CDS             11186..11469
                     /locus_tag="U4B19_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11831..>11945
                     /locus_tag="U4B19_002149"
     CDS             11831..>11945
                     /locus_tag="U4B19_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN2077875.1"
                     /translation="MNPFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
BASE COUNT         4106 a         1837 c         2220 g         3782 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 ttactatact cactatatta aatatattta gtatagtaaa aactagaaac aataaaattt
     6361 gtgaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
     6421 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
     6481 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
     6541 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     6601 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     6661 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     6721 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     6781 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     6841 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     6901 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     6961 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     7021 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     7081 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     7141 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     7201 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     7261 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     7321 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     7381 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     7441 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     7501 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     7561 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     7621 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     7681 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     7741 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     7801 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     7861 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     7921 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     7981 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     8041 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     8101 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     8161 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     8221 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     8281 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     8341 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     8401 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     8461 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     8521 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     8581 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     8641 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     8701 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     8761 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     8821 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     8881 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     8941 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     9001 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     9061 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     9121 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     9181 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     9241 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     9301 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     9361 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     9421 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     9481 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     9541 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     9601 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     9661 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     9721 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     9781 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     9841 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     9901 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     9961 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
    10021 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
    10081 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
    10141 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
    10201 gtatatccag tagtagtttt tatttactta aaaaattggt tcattgagca acctatcgct
    10261 gctaaagtag caaaggaaaa cgttaaagga aagaatttag ggaagaaatc gaaatgaaat
    10321 cggacgactg gattgaacac acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat
    10381 tttgggtatt gcaattatgg ccattaggaa aactttgtag ttacggggca cgacttattc
    10441 ttgatttaac cgtttactcg attggagtat ttggaacggc ttattacttg ataaatgatc
    10501 ccaataggat tgcagcatca gacattttta cagtgctaat gacgttgatt tttgtcatgc
    10561 tattgtctat ttttactcaa ttagtcatgc aatttaccag gcaaaaatta acagcaggaa
    10621 aacttttcaa aagcctattc gatttaattg ttgtaaatgc aattctagcg actttaataa
    10681 aacaaattga acaatctgga agaacaacaa aaggagtgaa ggatttttta ttgacgttta
    10741 ttcactttgt aaatggacac atgtatttgt ttgaagtggg tattattctc ttttttgtcg
    10801 ggtatgtatc cacgaaactt attaaacgtt caattgaaaa agaaattgaa attgacctga
    10861 ttccgacact tcttggttat gagtacaatg ttgattttgg aagatatgac gaattgatca
    10921 atacggatat cgttcacacg agtatccatg aatctactgt taagggcatc agatctattt
    10981 taaaaaatgg ccaggaaatg aaagcaatag atcgaagaaa agagatctac aatttttatg
    11041 aagtcgaagc aagcctacga tatggcatcg gaaaattgtg gttagatgga agtaatattc
    11101 gtcaaaacaa accatacatt ttttggaaca gtagtgattt agaacttcca atgaaagata
    11161 aataattatg gagaatcgtt tgaaaatgat tcctctatac aggattgtgc ttatagttgg
    11221 catggagtta aaaaccttaa agaaggagca gttattcaaa gtgaaagaac gtcataatca
    11281 gttactatgt agtaaaacgg gtacgatgaa cattgaatac cgaacagaat atttagaatt
    11341 aacgaatcaa actgattttt tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg
    11401 gctacttata gccaattgat tgatgaatgg gaacaattca aggaatctaa aattccagaa
    11461 cgtctctaaa agcctttatt tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa
    11521 aagtgttcca ataggctgtg cctattacgt ttctcaaaaa taaatgtggg aacaaaatat
    11581 ttaaaaatag gatagtgaaa ggatagtgaa ggatagtaaa aggagggtag taggatagca
    11641 aaaaggatag gctccttttc gctatccttt ttaaattgga ggaattaata tggaagtaag
    11701 aataagaaat gtggatgaga aaatcgtaag agaaattgat cgaagatgtg acgaacggtt
    11761 ctgttgcaaa gttttaaatc tactatcaaa taaggtagaa taatagaaaa agatagcagg
    11821 aggaatgacg atgaatcctt ttaaaggaaa gcaatttcag caggatgtga ttattgtagc
    11881 cgtgggctac tatcttcgtt ataaccttag ctatcgtgaa gttcaagaaa tcttatatga
    11941 tcgtg
//
LOCUS       DASJTC010000083        11941 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.084, whole genome shotgun sequence.
ACCESSION   DASJTC010000083 DASJTC010000000
VERSION     DASJTC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.084"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..498
                     /locus_tag="U4B19_002150"
     CDS             <1..498
                     /locus_tag="U4B19_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEN2077876.1"
                     /translation="WKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLR
                     KKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSIASAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVL
                     LGIPD"
     gene            complement(1113..2153)
                     /locus_tag="U4B19_002151"
     CDS             complement(1113..2153)
                     /locus_tag="U4B19_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN2077877.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2502..2828)
                     /locus_tag="U4B19_002152"
     CDS             complement(2502..2828)
                     /locus_tag="U4B19_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077878.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2815..3618)
                     /locus_tag="U4B19_002153"
     CDS             complement(2815..3618)
                     /locus_tag="U4B19_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2077879.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3971..4261)
                     /locus_tag="U4B19_002154"
     CDS             complement(3971..4261)
                     /locus_tag="U4B19_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEN2077880.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4397..4576)
                     /locus_tag="U4B19_002155"
     CDS             complement(4397..4576)
                     /locus_tag="U4B19_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077881.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4573..4923)
                     /locus_tag="U4B19_002156"
     CDS             complement(4573..4923)
                     /locus_tag="U4B19_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077882.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4916..6241)
                     /locus_tag="U4B19_002157"
     CDS             complement(4916..6241)
                     /locus_tag="U4B19_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEN2077883.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6691..7305
                     /locus_tag="U4B19_002158"
     CDS             6691..7305
                     /locus_tag="U4B19_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2077884.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7470..7712)
                     /locus_tag="U4B19_002159"
                     /pseudo
     CDS             complement(7470..7712)
                     /locus_tag="U4B19_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7916..9235)
                     /locus_tag="U4B19_002160"
     CDS             complement(7916..9235)
                     /locus_tag="U4B19_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HEN2077885.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9390..9977)
                     /locus_tag="U4B19_002161"
                     /pseudo
     CDS             complement(9390..9977)
                     /locus_tag="U4B19_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10615..10872)
                     /locus_tag="U4B19_002162"
     CDS             complement(10615..10872)
                     /locus_tag="U4B19_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN2077886.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10865..11134)
                     /locus_tag="U4B19_002163"
     CDS             complement(10865..11134)
                     /locus_tag="U4B19_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2077887.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11265..11444)
                     /locus_tag="U4B19_002164"
     CDS             complement(11265..11444)
                     /locus_tag="U4B19_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077888.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
BASE COUNT         3692 a         2155 c         1829 g         4265 t
ORIGIN      
        1 tggaaaaaga aaaataaaaa atccttttat tcctggaaaa tggatgaaac gtacatcaaa
       61 attaaaggaa aatggcatta tttgtatcga gccatcgatg cagatggttt aaccttggat
      121 atttggttac gtaaaaaacg ggacacacaa gcagcctatg cttttcttaa gcggttagtg
      181 aagcagtttg atgaaccgaa ggttgtagtc acagataaag caccatcaat tgctagtgcc
      241 tttaagaaac taaaagaata cggattttat caagggacag aacatcgtac cattaaatac
      301 ctgaataatt tgattgaaca agaccatcgt ccagtaaaga gacgcaataa attctatcga
      361 agtttacgca ctgcctcacc cacgattaaa ggcatggaag ccatccgagg attatataag
      421 aaaacccgaa aagaaggcac tctcttcggg ttttcggtct gtactgaaat caaggtatta
      481 ttgggaatcc cagattaaat catagatacc gtaagggatt ttattcttta tttaaaactt
      541 tgcaacagaa ccgtccgttg cgggtaatac cgtaacactg aagggcacgc attaaggcgt
      601 catcttaatg ctttcctgta ggacactgac ggagattacc agtctcccga gccttacagg
      661 ggcctttctt tttttgtatt ttcaattatc acaatttatc gtccaaaagt gcagaacgaa
      721 aatcaattca tcacaagcat atcagacaat taaatttaaa acaactaaaa atatacaaaa
      781 aagtgtaatt gaatattagc agcactgcct gaaaagaagt aatatcaggc ttttcagctt
      841 ggggaaataa gtgaattacc tctcttcatg aattattata actcaaaaaa gcacatatgc
      901 aaacactagt tccctatttt tttgaatgaa aaataactaa aatatctaac gattatgact
      961 ttaaaaccta cgtttgcgat tgttttcata aacttttaat ctaaaaactc cggaataata
     1021 gctatttatt tgtaacattt gtaataaaat taaaatcata aaaaagttct aatttaaaat
     1081 tgatcatttt tatagtgatc gctttttatc aactactcta gccaattttc cgtactaact
     1141 gttggtaatt cgttaaatgt ttctctttgt tcgtttgcaa gctttgccgc aatagctttt
     1201 tcgacaaacc aattttttac ataaacgaaa attagattct gcaaattgtt tactttttca
     1261 gttttttgtt tttgataagc tttcagcaaa gtcatatata atcctttgtc agcatcaatt
     1321 ccataagatt ccagttcttc ataaacaaca actgtttgct ctaattcttc agcttttttc
     1381 tttgcattat gaatagtttg gacggttttt tgagcgtctt cgtaagtact agaaaatgtt
     1441 tttatcaagt ttaatacatt ttctggtaca aatgtattta tactgtcctc tttcatgatg
     1501 tcgacgaagt tatctagtaa aacttgattt tgtaactgat cagtttcagt gtctattatg
     1561 tgtctattag tgtctataga attagaatag aatagattgt ctgcgatttt cgcaattacg
     1621 gttgagtcct tgatatgact ggattttgcc gtatttgcga ttttcgcaat tacggtggta
     1681 tcattgattt ctcgcggttt tgccgtattt gcgattttcg caattacggt ggtatcagta
     1741 ttgtcttcct cttccatgat tttaaagacg tcttgattcg ttacttctgg cttgcctaag
     1801 tacaataaat taggcatgga ttcaatctta ccgtcacgtt tatttactct tcctctcttc
     1861 tgtttcagaa gacctgcttt ttctaattcc tttttgattt tagatacttt accttcgcgg
     1921 cattgtaaca atttcattaa ttcttcaact gtgaatatga aaaatatatt atcatcagaa
     1981 tctacccagt tatttttgac tgaataatca aatcgatctt taagtagcat ataagcaatt
     2041 tttgcatcat ttgatagatc catgtatttt tcgttagtaa aaaatacctt aggcatttga
     2101 taatattttt cacgataaat ttcttttgat ttgtaataat taaaatcatt catagccaat
     2161 cctctttctg taggatagcg ttttgttcaa aactatgtta caatgaatta aacaaatata
     2221 tctatccact atatattttt ctactgctga taagtcgcca aactttgtta aagcagtagt
     2281 ctttttttat ctttttttag tttaccttaa aaaataaatt aaagcaaacc gctctatttc
     2341 aaggtttaag ctcatagtaa ctatatcacg atattacaat aatattatat cacgatatcg
     2401 taatattaca atatgaagat aagcgactta tcagctttga tactactgat ttttataaaa
     2461 aaaatatcac gatattatga tatcgtgata ttgaatgtaa attaaatttt attttgtttc
     2521 tttgctagtt tatatccttc aatcaagtat tcttcaggta tggactttaa tagcgcatct
     2581 tctataacgt ctggtttact tttgtctgat tcagcaatag tcgtccaata tttgagtgct
     2641 gacattagaa ttggatttat acgaatgcta tctgctgttc ttacactact tccttgatct
     2701 tttatgactt tacttatatt tttcttggtt aattcgattt cttctacttc ttttgattta
     2761 aaattctttt tatttttatt tcctagtaat ctacttactg cagcttgact gcgattatct
     2821 tctgccatca aaatcaccaa cttcttttaa atattcttct gtaaatgctt tatagtcatc
     2881 ttcagctgtt gagggtttat taaaattgat tagatctgat ccagcagctt gcgcttcgtc
     2941 aacggttatt gtcctacgaa tataagtttt gaaaattttt gcaccaatga ctctagaaat
     3001 ttcggttgct gcagacgtca tttctttaga aatgttataa cgcttattat acttagtaaa
     3061 tagaactcct cgtacttcaa gcttgtcgtt cgtatattct tttaccgttt caatagtttc
     3121 acttaacttt attagtcctt ttaatgagaa atacgcagct tcaactggaa tgataatttc
     3181 atcagaaaca gtaaatgcgt tgactgtaag cattcctaaa cttggtggac aatcaattat
     3241 aatataatca tagttgtcct tgattggttg aagtactctt ttaagcctgt gttcacttcc
     3301 tacttgagta aattccctct ctgcagaaga aagggctatg tcggctggca aaacatcaat
     3361 tcctttatag tccagaatag catctgtaat gtcagcctcc ccttttaata cttcataaat
     3421 tgtaggttgg tttgttccat ctatatcgaa acctacatta tctgtagcat ttccctgagg
     3481 atcaatgtcc actaaaagta catttttacc gtaaacaaat ttcaaacatg ctgctaaatt
     3541 aacgctagtc gtagttttag aaactccacc tttttgattg gctactgaca atattcttgc
     3601 cattaaaata tcccccattc atcttatatt ataatataat tatatcataa tattgtgata
     3661 ttgcaatatc gtatatgcat tatattgtaa tattgtgtga ttattttgtt ttaaagatat
     3721 atatcggttc ggatataaag attattcata tgttattcga aattaaaata atcacttttt
     3781 attactttaa aattttttat taatgataag aaatatgttt tgcgagcagc aactagaata
     3841 atttaaacat tactaaactg tctatattgt tactaataat tcccttctta tagtgcattt
     3901 tcttagaaaa aaacaatatt acgatatcat gatattaaga tatcgtaata ttatacaatt
     3961 gtgattgata ttatatatgt ttttttattt ttttatcatg tttaatcttg aaggaatcat
     4021 attctttttc taaagacagg agcttttcat aaacatcaga catgtttaaa ctagaggcca
     4081 ggcttaaatc ataaacgaat gaagcaggta atgattttat tctacgttgt cgggttatcc
     4141 agcttgcaac cgttccttgt gggtaggcat ataactcaca aaattgttct acagtgattc
     4201 ctaaattctt gataatgtaa acattgatcg ggtgcgagta aacaaatgta ttgatagaca
     4261 tggtgcgtaa gcctactttc ttttattatt gcgtttgcaa taatagttta acacacgcat
     4321 tattctagta tctactccta tttgtaaaaa caaaaaagga ttcatagaga taactctata
     4381 gaatccgtaa aataccttat atatataacg ggaagatata aaacctaaaa ttatgataac
     4441 ctgctgcagc ttctacatag aaagagaagc gctcaacacc aacctcattt atcaaacgtt
     4501 ggccataacc ccaaatctct tctgatagct gatcaaaggt tatatacttt agctcatatt
     4561 gctggataat gttcattcta atcgactcca tttattatcc tggtaaaatt cgatatgacg
     4621 tatttcgtcg tagtgaattt tgtcttttcc aacgtagatc cccagctcat cactacctaa
     4681 gatcttgcca atgatatctt ctggatagta tccttcagcg tttcgttctt ctttttgaat
     4741 agatactgat ctatttttta tgactgcagc gttcaatact tccgcaatct cttcagtcgt
     4801 catttgttct tttgctaaat tttgactggt tcgttgttca ttgtcggcat caatttcttc
     4861 agtatgatca gataaataaa accctgccca ctttaaacgt ccacgatcat tgtaatcaat
     4921 catttttaat tcccccattt ccgcccgcat gaccaccaac aagtgtgctt ctagtaatgc
     4981 ttctagcgcc ttctaacatt gaagaagcat gtacaatcga cgtaaaacca tatttttgcc
     5041 gaatcttgtc aataatttta tctaaatttt cttcgtcaat ttgcttttgt ggatcactaa
     5101 ataaatccag ctgaagcgaa tcggtgtaca caagcttgct ataggttatt ccaacatgcc
     5161 ttacttcttg tccttcataa tacttatcaa acaagaaaag acaataattc tgcaattcct
     5221 ttgtattatc tgtgataggg atcttcatct ggtgtgaaaa ccctggtctc gtttctaaga
     5281 tcgacgtgcc aatgtgtaaa tggacacaac ctgttttaca gttatgctgc ctaatgcgta
     5341 tcgctacttg ttccgccatt tcttttacca ccagctcgat ttcatttctt ctagtataat
     5401 ctcgtggtaa cacttgtgaa tttccatatg atttttcctt tgtcggttct ggtgggattg
     5461 ctatatctgt tcgatcgatc ccattagcat ggaagtacag ctgtaagcca atcacaccta
     5521 gtctattctt aatcgtataa ggattccagt tagcaagatc tctaatgctc ataatcccca
     5581 tctgattgag tctttttttc attcgagagc caattcccca aaagtccgtc atttcaggaa
     5641 tattccatac tttttcagga acgttttcgt acgtccattc cgcaacaaat ccttcattat
     5701 gctttgcttc gttgtctaag gctagtttag ccaaaagggg attatctccg acacctactg
     5761 tagcaatcaa tcctagctct tctttaatat tctcctggat catttgagcc agcttctttc
     5821 tccgttgact tcgtgttcct tcagtcgtaa aaagattcag tgatcgggtc acttttaaga
     5881 ttgattcatc gatcgagtaa atcagtagat cttcatcagc cacatatctt ctgaaaatat
     5941 tatttacctg catatttcgc ttgatatata ggttcatacg tggtggaaca acgtgtagtg
     6001 ttttaggaaa tggttgtggt aagtcacgtg gtctactcac atttgtaata ccataccgct
     6061 tttttgcttc aggagaagaa gctaatatca atcctgaacc agtattatca gctcgactca
     6121 taacaacaag ttctgttgtt aatggatcta aatttctttc tatacattcg acacttgcgt
     6181 aaaagctttt catatcgatt aaaaagtaat catttactgg ctcttttgaa taatccatca
     6241 taaataacac tccattcttt cacattatac aaacatatgt tcgatattgt aaagaagaaa
     6301 tagctaaata cataaaaaaa gaagcatagg tatgctatca ccctgcttct tcttttgatt
     6361 tgtatgatca atttattcta tcactttact aattaatttg aaaatctaat aaaaactctc
     6421 actctaaaag agtgagagac tgcaaattag agtacggact tttttggagt tccgttcttt
     6481 aatattaaca aatgaataaa atccttacaa catttgagaa taagttatca gtagacactt
     6541 acaaaaaatt ctcatactat ttcctccatc attagttttt gtctatataa ggtttaccct
     6601 aaatagacac tggtataaca ttgaaaaata tggcttcctc gtgtatgatt agggtgtatt
     6661 ttaaatagtt gcagaaagta ggtaaaagtt ttgagagttg catacatacg tgtctcatcg
     6721 atcgatcaaa atgaacaacg ccagattgaa gaaatgaaaa aattcggtgc agagagaatt
     6781 ttcatcgaga aacaatctgg cgcaacgatt actcatcgac ctgttttcca agaagcatta
     6841 gattacgtcc gagatcaaga tatttttatt gtggaagcta ttgatcgact tggaagaaat
     6901 tatgacgaga tcatcgcttc agtcaattac ttgaagaaga aaaatgtgaa gctgatcatt
     6961 actagtttac cgatcatggc tgaagcaatt ggcaatcctc tcctggataa attcattaag
     7021 gatctgatca tccaaatttt ggccatgatc gctgaacaag aacggactga atcgaaacgt
     7081 cgccaggcac aaggaattaa gatcgcaaaa gcaaacggcg tctacaaagg gcggcccaaa
     7141 ttatacagtg cagacgcaaa agatccccaa cgtcgccttg tttacaaaag catcgttgaa
     7201 gatcttaaaa atggtgttgc gattgctaag attgctaaag actacaatgt tacgagacaa
     7261 accgtgtata gaataaaaaa agacagcatg gtcaatgaca aatagttgat catgctgctt
     7321 ttattatctt ctcatcttta atttcctaca tataattact atctataaaa ttaagtctta
     7381 ctcagaacag ataaagcata aaattccaac aaaccatttt ttacttaact aaataaagag
     7441 tctgttaata taatcttcac ttatttacgt taatccccta ctgtatttgt tggttcctta
     7501 tctatatttt ctacaaaatc cttattatct tgtgatttag cttcgtataa ttctttgtat
     7561 gtcgacacat tggttatact aattcctata gctagttctt gagactctac ttttctctct
     7621 agctcctttg acaaagtgat ttgagattta tttggttccg ttggagtcaa ttctatagat
     7681 tcttcataaa aatctctata cataaagcta tccctgaata attcatagct ttaattcttt
     7741 ggcacatttt attgaaatat gtatcactaa tatccctatc aactgatttt gactaaaaag
     7801 ttacttcaaa cattcactgt tcaaaaatat tgaggaataa attttgggtg taaggcattt
     7861 tgagcatact tctccctgga taacttccca gtaaaaaaat cgttagattg ttggattaga
     7921 tccattgact ggctcgcctc aggcgagcaa agaccctgta gaattcattc tgatacacat
     7981 gataactaga taatttgaga tagactcgtc tacccgtttg gactaatttc ccagcaactt
     8041 tcagaaactt caatcgaata gaatgaatgg tcattccctt ttggacttct tcaaagccaa
     8101 tcgttcttaa gaagttgact aagttgtaag ctatcaagct gagcatcatt cggacatgat
     8161 tctccaaaaa gcgaggactg tctgttttgt caaagtaaaa gccagctttc gcttctttaa
     8221 tgaagttctc cattgtgcca cgtttggcat agatagaaaa gatggtatca ggagaaacat
     8281 tttctgatag attcgtcacg ataaattcat gtcgaaagag tagttcgccc gcttcacgtg
     8341 ttgagcgtat acacacgcga cgactttgtg accaggattg tgcttgataa gtggtcgaga
     8401 agtactgaac ttctcgttct tcccactttt gattatcgcc ataaagtact gatttctcag
     8461 ctatttgacc tagtctacga ttattcttca gtcgaataac ataatgactc ttttttgatt
     8521 cacacgaatc atacacacca ggtgtagcga acccgctgtc tccacgaacc aagatgtcag
     8581 tgtttggtag agagtgatta tagtgctcta ataaaggtgt gagaaactcc tttacgcctt
     8641 ttgatgtata ttgatttcct gaacgtagtt cagcttttaa gaagtcacca gtcaatccgt
     8701 caaaagctac caatggatgg taaccatagg tttggtagtg tgtattataa ttcgtttgtt
     8761 cttggtgacc aaatgtatct gaatgggtcg aatctaaatc aatgattaat tccgtatcat
     8821 tacggatgag gcgtgcttta tcgataagtt cttggttcaa agcttgaagt tcatggatat
     8881 tctcctcaga tagtcgatct aaaaatcggg aaagtgaaga ttgagaagcg agttctttcc
     8941 tatctaaaac agctttaaac acaggatctt gtctcaggag attagctgca gaatcagctg
     9001 agtaaccagc aattaattgc ataatgaact gctcaagtat ggataagttg tcatgagtaa
     9061 aatacgctcg ttcgtcttca atgtgaatgt gttgcttagc cagttcagaa aaaccaaaga
     9121 tgttcatcag ctctttcact aggacgagac ccgaatcact cgataattga ccacctgtat
     9181 gagaaatagt gatatttgaa ttgaatttta cttggttttt gtgtaagcta gtcatgaaaa
     9241 gaactccttt cttttggttg gtttagtcac tttaaccata gcagaaaggg gttctttttt
     9301 catcacttaa cgggtgaaga tgaaaaagta attgtaagct gccgtgaggc agtttcacat
     9361 ggttttaatt aaaagtatga attattcagg ctatcattaa ttttataaaa aaaattaaat
     9421 gtttttagca ctgccatata acattcaaaa cattcttcta tagttggttt acttccttga
     9481 ggagaatgaa ccaatttatt acgtttttct atgaaatttt taatttcctt tcttatttct
     9541 ttcggaaact caatattaaa gtacccttta aacatgctcc ctaataattt gtctacaggg
     9601 ggagattgta aattctcaaa tattagagtt aaatcaggtt tctcttgttg ataaaattcc
     9661 tttaccccta tttcacaggc tgtaattatc gaaataaacc tatctctagt atttgatata
     9721 tttttacttc ttttaaggag tataagagaa aaggggatac tttcgtgtcc atgcatgtaa
     9781 ttaactaatt gttgagcttc agaagttata tctctaatta tatagttaac ggaattagga
     9841 agtgtcttta actctaatga aatttctatc caattactat cttctaatct gtatatgctc
     9901 gaagtataat ttgattcgtc agcaagcatc gctacatccc aaacactgat cataaaatct
     9961 tttaaacgtt caatcatttc agcaatattg ctaaaatcag ctaatttttc tttagaataa
    10021 aaaaaaacat ttatatcaga atcaataaat tctatttttt tattactata tcgtcatcat
    10081 tatcttgacc atttatgttt attgttccct gagtggggag tacttctttc aaattcaagt
    10141 taaatttata ttccattact tctcctccaa tatataaaaa ttacttaact gtatccaaat
    10201 ttaatatgta aataaatcat taatttttag ctaaattcta tgagaagttt atttttgttg
    10261 tttttgctaa ttaactaaaa ctagtacaaa aaatttaaaa tagattatta aacctattgc
    10321 ttattctatt cagtcatata tataagcgtc atcctttagt aactaacatc ccacactaca
    10381 atagtattat aacatatcta ttcaacttca aattgttgaa atttaagcca tttatattta
    10441 cttttcctca actttagaaa tgattttact aaccttatat ccgtaatacg cgtaacaaac
    10501 acattcatta ataacaatca acactaagct atattagctt tttaaatgac tagggtaaat
    10561 aaaataagga tcaaaaactt ttaagtttct gaccctttcc ttacttccga ttggttaata
    10621 gtgatctttt gcagaataaa taaatatcgt ttcattttca actctatata tcagtctatg
    10681 ttcatctgta attcttctgg accattttcc agataaatca tgctttaatg gctcaggttt
    10741 tcctaatcca gcaaaggggg aacgatcgat atcttttatt aacttgttaa tcttttttat
    10801 attgcttttg tttccttgct catgccaata aagataatca tcccaagcat catcagacca
    10861 agccttaatc atcagattca acctcgatta agtcatgtgt tttaaatgca cctttggaga
    10921 attgttcatc tcctcgacga attttttcca tgacgtaatt attagaaagt gttctcaacg
    10981 tttcttgcat agaatcataa tctctttttg ataatacaac aactgtatct tctacatctt
    11041 tacttgttac aataagtgtt tcagcatcct cattaacttg tttcatataa ctacgtaaat
    11101 tttggcggaa atttgaataa gctactgctt ccattccttt caccccttat ttatttctat
    11161 aaaacaattg tacattatat tgtacaatta agcaaatgat taatattcta acttcttata
    11221 attaaaagtg ctatcacttt tagtttttag gataaaaagt gacgtcactt tttgagtgct
    11281 ggtacctcaa atcctgcttt ttcttgggca atcaataaat ctaacgcaat acacataatc
    11341 ctggattcac ttttgccgta cttctgcttt agttgctgca attctttttg tctcttttca
    11401 tccaacgtaa cgggtaaccg tttatttcct attgcaattg gcattagtac tctctccttt
    11461 catcgaaata accctatgaa aaatatggct gcaatcatct tgaatagtag actcacaagg
    11521 gctaatacaa agcgccaaaa gatcaaacaa aataccagta ttgccaaaaa tactacgaag
    11581 tttaaatgca cagctgcagc atggcgcata agtatttcgc caacaaatag gactaacaat
    11641 agaggaatga ttttattcat atggatctcc ttctttctct taggttatcg ggtgttgccc
    11701 gcttatttcg caccacctaa gcggcggggg cggttggagc gattggtgcg aaataagaac
    11761 cattccctta gttactaggt tttttgtcct tatttcacac attctcactt ttcggtataa
    11821 atgattattg cctttttttc ctttcaaatc gttgatatgg cattcgttta cggtcaagtt
    11881 cattttggat ttcttctgta tcgtcgtaag tataggtgtc taaaaaatca gttaaagtcg
    11941 t
//
LOCUS       DASJTC010000084        11766 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.085, whole genome shotgun sequence.
ACCESSION   DASJTC010000084 DASJTC010000000
VERSION     DASJTC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.085"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            506..1069
                     /locus_tag="U4B19_002165"
     CDS             506..1069
                     /locus_tag="U4B19_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077889.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1130..1495)
                     /locus_tag="U4B19_002166"
     CDS             complement(1130..1495)
                     /locus_tag="U4B19_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077890.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1571..1816)
                     /locus_tag="U4B19_002167"
     CDS             complement(1571..1816)
                     /locus_tag="U4B19_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2077891.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1886..2140)
                     /locus_tag="U4B19_002168"
     CDS             complement(1886..2140)
                     /locus_tag="U4B19_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077892.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2304..2627)
                     /locus_tag="U4B19_002169"
     CDS             complement(2304..2627)
                     /locus_tag="U4B19_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077893.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2814..2887
                     /locus_tag="U4B19_002170"
     tRNA            2814..2887
                     /locus_tag="U4B19_002170"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2848..2850,aa:Ile,seq:cat)
     gene            complement(3272..3769)
                     /locus_tag="U4B19_002171"
     CDS             complement(3272..3769)
                     /locus_tag="U4B19_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2077894.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3809..4618)
                     /locus_tag="U4B19_002172"
     CDS             complement(3809..4618)
                     /locus_tag="U4B19_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2077895.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4952..5809
                     /locus_tag="U4B19_002173"
     CDS             4952..5809
                     /locus_tag="U4B19_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN2077896.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5802..6704
                     /locus_tag="U4B19_002174"
     CDS             5802..6704
                     /locus_tag="U4B19_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEN2077897.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6737..7087
                     /locus_tag="U4B19_002175"
     CDS             6737..7087
                     /locus_tag="U4B19_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2077898.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7136..8059
                     /locus_tag="U4B19_002176"
     CDS             7136..8059
                     /locus_tag="U4B19_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEN2077899.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8137..9030
                     /locus_tag="U4B19_002177"
     CDS             8137..9030
                     /locus_tag="U4B19_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2077900.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9068..9631
                     /gene="amaP"
                     /locus_tag="U4B19_002178"
     CDS             9068..9631
                     /gene="amaP"
                     /locus_tag="U4B19_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN2077901.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9645..9833
                     /locus_tag="U4B19_002179"
     CDS             9645..9833
                     /locus_tag="U4B19_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN2077902.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9846..10748
                     /locus_tag="U4B19_002180"
     CDS             9846..10748
                     /locus_tag="U4B19_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2077903.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10780..11022
                     /locus_tag="U4B19_002181"
     CDS             10780..11022
                     /locus_tag="U4B19_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2077904.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11278..11649)
                     /locus_tag="U4B19_002182"
     CDS             complement(11278..11649)
                     /locus_tag="U4B19_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077905.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4168 a         1983 c         2232 g         3383 t
ORIGIN      
        1 attggtcgtt gaatgggctg aatagtagtg tataaaacaa ttttgtggag cgtgaagctt
       61 ttatggtaga ggaaaatacg aataaagtga tgttgataaa aagattccta tcttgaatat
      121 gaatcgagat gtcttgaaaa tgaatgagtt tgaccaatca ttatcctatc gaaatttaca
      181 gtttcgttcg attggtctgt caattaatga aaaagtaaaa aagaatatgt tctaggaaga
      241 gggcggcaca tctaaatttt taagtaacgg acagttcaaa tatcaagcat tgaaagaggc
      301 tatcctactg tttgaaggaa tacgaatata taaaaacgac aaagaggaaa aagtagttca
      361 ttaaatccct tcaagtgatg aaagaatgag atctaataaa acctgatctt tcatctgttt
      421 ttttaataac gtaataattt ttttattttt tgtgctttcg ccgttattgt agagtgattt
      481 agcattctta tttttaaaaa taactgtgtt atatattaaa aaaggcataa tagttataag
      541 cttttttaaa gaatatttag aaattattaa ttttcgtctg aaaacaaaaa aagaaagcgt
      601 tattatttta gtctattttc agggggggaa ccttatgata aaaaaactat ttttactatc
      661 aatattaacg ctagcaatgg caggttgttc tgactctggc ttagcaactg aactgatcac
      721 aaaatcaact gaagccacga cagaggagtt aaaatgtgga aacgatcaca gtgactacag
      781 aggcttaaat cgacgatgca tgggaatatt aggcagaagc aattgtaagc atcgatgtag
      841 taatcgaaga aggtgtagaa gaagcaacag aaaaatcaaa agaaagatca aaagaagcaa
      901 tctgtgctgt gcatcgtata cagacgtcga agatgcggta cttttaaacc acgtcaggag
      961 atgttcattt atgtctaggg aagttggcga tgaaccaaaa ctagagtacg cttttagttt
     1021 tgcacgtgag gatgaagata accctaaaac ttacgacatt ttagcttgat ctaagatggt
     1081 taaagctttt taatgaagta agaggagata gaaggctttt ctagttatat taggtaagat
     1141 aaattccttt ttgctccaaa tttgctaaac attctgctag ataaacttca tcgtgttccg
     1201 gataaatcgg cacgttgcgc tctacagtag gtaaatcaaa aaatgcttcg catgatttgc
     1261 cacgatacat aggatttttc cactctggtg ctgggctgta accacgtttt tccatttcat
     1321 ccatgacaag ctgatgatat tggaataatc tataaggaga atagtgaaaa acataatcca
     1381 cagtagcatg tttctttccc cagccatttc ctcttaatgc acagcattcc cgatgttgtc
     1441 ctaaaagctg ttgacgagga agctttgaaa tcaatttttc atgccataat cgcatagaaa
     1501 tgtctccttt atatatagat tgttaacctt agacgttaaa tgaaaaaacc agcaccaagg
     1561 ctggaaaaaa tcacttcagt ttgcttgctg tttctgagcg accaattagg taatcaacgg
     1621 aaatatcgta aaagtcagcg atatagatta attcaatgat gcttggaatt tcttctcctt
     1681 cttcgtactt acgatatttg aataaaggaa tacttaacgc gctagaaaca gctacttgag
     1741 ataaagatcg gccagttctt aagaaattca gacgacaacg taaagtatcc agtaaaatag
     1801 cttcttcgat actcatttaa tcacctttca ttatagtaga atagttataa tgactataga
     1861 aagctacaca tgcaatcaat agtactcaat gttttttatt taaacgtatc aaataaagaa
     1921 tgacgatcaa taaagacaaa accagtcctg ataattcttc tataaaaaca ctatcgggtt
     1981 caaagtagtt gtaattaaaa actttccaca taaagtagat agccggaaga gtcaaaatag
     2041 ctaaataaaa ccgttctttg ttccaattca taagataaac aatacttgcg accagtgcga
     2101 ccgcaggcac ccaaaacaaa actctcacca acgtaatcat gtaacggact cctcaacgat
     2161 tcatataata aaagataaca tgttattgca tacatcacaa atgaaatgct aggaaaataa
     2221 aagaagaata gatcaatgaa aaatgaaaaa gcagaacagt tttctgctga taagcaggaa
     2281 ctgttccgct gaatgtgtct tatctatcga gaaaaaatca atggattgat atctaagtaa
     2341 ttcaatacat ataaaaagag gacgatcgca ttcactaaaa aggaaagaat gatgatccca
     2401 tagcgaatgg aaacatattt aacaaatact tttgtttgaa tacgggcata tctttttcct
     2461 ttatagctga gcatcatgac aagaagcaaa agggtaatca caccttgaaa aatcattcca
     2521 ataaacagaa ttcctgaggt ttgataattt aattggagaa aataagctgc tgccgcaact
     2581 aaatcgaata aaatcgcaat aaatggcaaa ataatcactc ctaacattga gaataaataa
     2641 aagaataaca ttttttctaa aataagcaaa taaaaattaa agtaacaaaa tagttatgac
     2701 acaagtaaaa gaaataagaa taacaatatt attttactta tattttgaaa cttgtgagag
     2761 tttgtcattc gtgctatagt tgaaaagtaa caaaaataaa ttttatttat gatgtacctt
     2821 tagctcagtt ggttagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca
     2881 aggtacataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa
     2941 aaacagcttc ttatgttttt acttggctgt tcttattact gcagatgcaa gcttttgaat
     3001 accgtttaag gaagaggttg tgaaatcagc gttttgacct gagtattagc ttaacaaacg
     3061 gaaaaatagt tcctcatatt ttgccgtttg ttaagctaat accgagggtc agctgattga
     3121 acaccattta agaaagaggt cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa
     3181 tctactttga tagaaaaagc acgtaggact aaaacagttc tgcgtgcttt ttttattgtg
     3241 tgttaaatat cgtaaatgaa gatagatatt tttatgattc gacgatagag acgttgaaat
     3301 ctttcaacag ctctaaagcg gattctccgt gttttctact ggagactaac attggatcaa
     3361 caaaaaagtc tgcttcagga aaagcactct gcaggcctat cagattagag agtacacaca
     3421 agtttgtttc aatcccgact acttcgatct ctttttgttc ttctttgctt tcaaataaag
     3481 tttcctggat ttcagctaaa gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc
     3541 gttcattttc ttgtggagaa agaagaggga tcaattgcaa atcttctcgt tcttcttctt
     3601 tgtccttcag ttcaactggt atatcccgtg tgaagagaat atattcatta ttttggcgtg
     3661 ctttagctaa tctttgagca atacgttctg ccaattgatc tgactctgga atataaaatt
     3721 cgctgtttgg atcaagaata tgattttgca tatcaataat aactaacatt ttctttcctc
     3781 ctacattcat atcgtcattt tattcttttt aatagaagtt ttttaaaata taagcaacac
     3841 cagcttcttc attcgtcagt gttacttggt ttgcaatttc ttttacttgt cgctgtgcat
     3901 tccccatcgc aatactcaac ccgctgtaat tcaacatagc aatgtcgttt tcttgatcgc
     3961 caaaagccat gatgtcactc gattcgattc caaggtaatc cgccagaaaa acaagtgaat
     4021 gagctttagt gatacctgta ggcgtgattt ctaagaaatg cggatcagag aatactgatt
     4081 ggataccatc tgcttgcaga tctctttgta gcttctgtag tttttcttca ctcttatctg
     4141 ttaatgctaa tttaagatag ctaagagaag gatcttccaa catatttaaa atttgatcaa
     4201 aagaatggtt ctgtaaattg aaatgttcat aataagggtc atctttgact agatttgatt
     4261 catcgaatgt cagctgatca tcttgggttt ctcggctagc aatgatggat attttcgctt
     4321 cttgagcata gaagaaggct tttctcagtg tatcgctatc tatttttgaa cgtctgagaa
     4381 ctttcgccat ttttccatcc ttcatttcag caatcagtga accattggaa gaaatcaaat
     4441 agccaccgat tcctaattcg gaaaaaatat ctttagcaga aatgctgttc cttccggtag
     4501 cgacggttac gattccgcca ttttcatcaa atttccgaag tgcttggata ttttcttctg
     4561 gtatctttcc atttcgatca agcaaggtac cgtctaaatc aatcgctgct agtttcattt
     4621 tatcttccta ctttcttttt acatatctat tttcctgtta ccttaagatt aaatgagaat
     4681 ttaaaaaaat gaaactaact tgctttgtca ttatgaattt tttcaaaaaa cattcttaag
     4741 aaaaaggtgg ctatatatag tagtttttgg tcaatacctt tgaataaaaa gccatgaaaa
     4801 acgaatagtt agcgcttacc gatgcgtttt gtgataaaaa aattttaaaa tattcaatga
     4861 aaacaaagca tatattttgc tttaaaacca aatgaggaat attctagatg tagaacaggt
     4921 tagaaaaaga ttagaaaaag gagtgatttt gatggagaag agatacggcg tagttcctgt
     4981 ggtaactgct tcagacgaaa attatgctcc ttacttgagc gtgatgatcg caactgcatt
     5041 agaaaattgc aataaaacaa gacgcataaa attttatgtg attgacgatg gtctatcaga
     5101 atatagtaaa gaaggattgg aagaaacggt aaataaatat tcaagtaatg caagtatcca
     5161 attcctgaca gtggaaaaag atatctacga agacttccta gtcagtgacc atatcacaac
     5221 taccgcttac ttaagaattt ctttaccaaa cttattagct aaagaggatt ataagaaagt
     5281 cctttactta gattcagatg tgttagtact agacgatatc gtaaaactat atgatgagcc
     5341 gttaaatgga aaaacaatcg gcgctattat cgatccaggc caagtgaaag ctttggaaag
     5401 attaggaatc gattccgatg acttgtactt caattcagga gtgatggtga ttgacattga
     5461 tcaatggaat aaaaaagaaa ttacagaaaa aactatccat tacctcagtg aaaatggcga
     5521 tcgaatcatc tatcacgacc aagatgcttt gaatgctgta ctttatgaag attgggaaca
     5581 acttcatcca aaatggaaca tgcagacttc gctgattttt gaacgtcatc cagcaccaaa
     5641 tgaaaaatat gaacgtcagt ataaagaagg aaatgaaaaa ccttcgatcg ttcactttac
     5701 aggacatgat aaaccttgga atacgttgaa agatcatccg tataccaatt tatacttaaa
     5761 aaaattagca cacagtactc taacgaaagt aggcgagatc aatgaataaa aaagaaatag
     5821 cagtagtcgc aagttgtaac acaaaattcg taccacattt agctgcatta tttgtctcag
     5881 tattagataa ttgcaacccg tctaaatttg ttcggttcta tgtgattgac gatgatatcg
     5941 attttgaaag taaacagctt ttacgcttct cagtgaaaaa cgcacgaatg aactcagatg
     6001 tggaattctt gaaaatcaat aaagaattct ttacaaatgt ggtaatcagc gatcgtattc
     6061 cagaaacagc ttattatcgt attgcgattc cggaattgtt ccgcggcaca gaagttgaac
     6121 gtatcttata tatggattgc gacatgatcg ctttacaaga tatctctaaa ttatggcgtc
     6181 ttgacttcgg tgattcaatc gtagcagctg ttgaagacgc tgggttccat caacgactgg
     6241 aaaaaatgaa aatttcagct aaatcaatgc gttatttcaa ctcaggtttg atgctgatca
     6301 atgtgaaaaa atggttagat gaaaatatta cgcaaaaagt attagatttc atcgaacata
     6361 atccagaaaa attacgattc catgaccaag atgcattaaa tgctatctta catgaccgtt
     6421 ggttaccact tcatccgcgt tggaatgcac aaggctatat catggcaaaa gcaaaaaaac
     6481 atccaacagc agcaggtgaa cgtgaatacg aagaaactcg caacaatcct tacattatcc
     6541 atttttcagg acatgtgaaa ccgtggagca aggattttga agggccaacg aagaaatatt
     6601 acgaaaaata tgcagggatg acggctttcc gttgtgtagc taaattccct aaatatccaa
     6661 aatatgcaaa agttcaacca aaaacagatg tagttgttaa ataatagaag accacaactg
     6721 aagaggggga gtaggaatgg aaattacaat gtctaaaaat gcagtagaga cgttaattga
     6781 aaaggttggg gaaaatacaa aaatcgcact cgcgcttatc aatgattccg atccatttct
     6841 aagggacaaa ggtgcttttg caaaaggcag cttttttcaa atcattcctt ttgtttcgga
     6901 atttggagaa tatgcgacta aaatcgagca tccattattg gacatctata catctaaatt
     6961 agagcaaaat tatttcggta aacgattgaa catggacttc aacaaacagc ttgacagctt
     7021 ttcattagaa aatgaaatcg cagtactcga ttataatatc aaattaaaga actgcttctt
     7081 cagctaatag tttcgagaaa gtccaaaaag gaggtataca ttgagatact actttgtgga
     7141 tgaagaaaag aatgtaatag aaacagatga aaaaaaatat aattggctcg tacttgatag
     7201 tgcaaagccc gaagagattg agcaagcaat cgaagaattc gagcttcctg atgatatttt
     7261 cgtaggaacc gaatatccgg aagaagtttc tcggatggag cgcctttatg agacgaaact
     7321 agagcatcca ttttctttgg tagtgattaa tctagatcag tcagataatc gtatcgaaaa
     7381 aaaattaacg ccaatatctt ttgtactttc cgataatctc ttgattactt gtatcgatag
     7441 aaaaaccgaa tttgtggatc ggctgctgac aaagcatgga aatcatttaa actcgtttga
     7501 aaagatcatc acgtatacat tattagatat ttacacccat tacgtgaaag aactaagaga
     7561 gatgaaaaag cgaattgacg ctttggataa agcagctcgt aaaacaacgg aaaatgaaga
     7621 actgtataaa caggcagatc ttgaaagaga catcgtatat atcgatcata ccttaagaga
     7681 tcaacgggaa acaatggatc aattatggaa ttctgaaact ttcatcaaac gtttagacga
     7741 tgaacagcta ctctatgatg tccatttgag acagcgacaa acagaaaaaa tgatcgagat
     7801 ctatcgagat ttattagaga gtattggtga tctattcaac gggatgatgg acaataatct
     7861 aaatcatatc atgaaatatc tagattcagc tgcattaatc atttccgttc cggcgttgtt
     7921 cgcagggatt tggggaatga atacaggagg attaccgggt aaaagttctg ctctaggctt
     7981 tatactcgta gtctttggct ctttgatagc agcagtagtt ttaggttacc atttataccg
     8041 gaaagattat acaaattaaa gaatctattt aaaacaggta tcaaacaaag aaatttgttt
     8101 tggcaataca atttaaaaac taggaggaat acagcaatga cagaaccaaa attaatcgac
     8161 ccgcgaaact tgtaccatac ggagaaattt cctaaacaag atcaggatac accagcttta
     8221 cagcagaata tgattcctgt tcctgattgt ggagaagaat cctatgaagg caataatcaa
     8281 ctagaaaacc gccgtgtact aatcactggt ggagattcag ggatcggtcg tgcagcagcg
     8341 attgcatttg ctagagaagg ggcagatatc gctcttcaat tcttcccagg agaagaaaaa
     8401 gatgcagagg aagtagcaaa atacgtgaaa gatgcaggac gtaagattgt cttactacct
     8461 tttgatcttc gtgacgaaca agcacctaaa gaaatcatag ataaagccgt gagtgaactt
     8521 ggcgggctag atacgatggt actaaacgca ggacagcaaa tttcttgtca gtctattgct
     8581 gagttaccac ttgagcaagt caaagacaca tttatgataa acatcatcgc tatgtttgct
     8641 ttagtgaaag aagctgttcc gcatatccca gcaggaggag caatcgtaac aacaacttct
     8701 gttcaagcat tcaatccaag cgaacattta ttagattacg cagcaacaaa agcttcaatc
     8761 gctaacttta cgatcggttt agcaaaacag cttgcaccta aaggaatccg tgtaaatggt
     8821 gtagcacctg gtccaatttg gacaccatta caactagacg aaggacaacc ttctgattcg
     8881 atccctgaat ttggtcagca aagtttacta gaacgtgcag gtcagccagg agaattagca
     8941 ccagtttatg ttttcttagc atcgaataaa gcaagttatg ttacagccca aatttacgga
     9001 gtaactggtg gagaagcgat caacttataa gaagttgatc gtttcacccc tatgaggagg
     9061 tgcgaaaatg aataaaggtg taaaaacgat cggagtgatc ggaacattac ttttgctatc
     9121 tgtgcttttg tttgcgatac tattaagtgc aacctatcca ttgccttttc agctagagaa
     9181 gtttcgattc ttttccatca ctaattcata cgtgcaacaa tatgtatttt ggcttgccgt
     9241 tgcatttgct attttgacaa ttatcacgtt acttgttttc ctgttttatc caaaaacaat
     9301 cggaaacttt ctactgaaac atggtgatgg ggagttgacg ttggataaaa aagcaattga
     9361 aggaatggtt cgttctcact tgcatgaaga tgaattcatc cattcaccaa aagtaaatgt
     9421 caaagcaacg aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac gaacttcagc
     9481 actcattggt aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac aacacatatt
     9541 gggaacaaaa gagccagtga aagtatcggt gaaatacaca ggctttgaag aaccagctaa
     9601 acgaagcaat gaccgcaaac gagtagaata gggggggacc aaaaatgaaa gaactatttt
     9661 cgacatacaa gctccctatc atctttgggg tacttggatt gatattagcc gttttatgga
     9721 tcaccgtggg ctttttcaag accttattga tcttgatcat ggcagcgatc ggcgtcgcta
     9781 tcggttttta tctgaaacaa acgaaaatct tagatcacta ttttaaatct taggaggaaa
     9841 catccatgaa taacaataac aaaaataata acaataaaat gaacaaccaa aaaccagtgt
     9901 ctgcacatcc agacccaacg acaccaaacc aatcaaatgg tgtgaacgat gaaaagaaca
     9961 aaacagcacc agtatctgca catccagatc cagtagtccc taacgttcaa caaggagtag
    10021 aagagaagaa acatgatgca gaaaagcatg cacctcatca tccaggaact ccagaatcta
    10081 aagttccaga acaacatgat gcaatcgatc cttctaaacc tgtggaagag caaaaacatc
    10141 cttctgacca tgcgaaagca caagaaaaac ttagttctga ccacacaaac ggacaaggaa
    10201 aacttggttc tgatcataca aataaccaag gaaagcatga ttctgaacag gaagattcaa
    10261 tgcgcggtga gctgaattat gaagataaag ttgtgcaaaa aatcatcgga atcgcaattg
    10321 aacaagtaga cggtttatta tctgcaaacg gtggtttctt ctctaatgtg gcaggaaaac
    10381 ttgtgaatac agataatgta acggctggga tcgaaacaga agtcggtaaa aaacaagtag
    10441 cagttgatat ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc tttgaagaaa
    10501 tgcaagaagt aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg atcgaagtca
    10561 acgctaacgt agtggacatc aaaacaaaag aagaatttga aaaagatcaa gaaacggtgc
    10621 aagacaaagt aactgacgca gcgaaaaaaa ccggtgaatt tgcttcgaat caaacagata
    10681 aagtaaaatc agcagctgga agcggcgcac aaaaagtaaa agaaaataca gaaccacggg
    10741 ttcagtaata aggaagtaaa acattaggag gaacgaaaca tgggatttat ttggtcactt
    10801 atcgtcggag gaattatcgg agcaattgca ggagcaatca cgaataaagg ttcttcgatg
    10861 gggattattg caaatgtcgt agcaggttta gttggatcag cacttggtca agcattatta
    10921 ggttcttggg gaccaagtat tgcaggaatg gcaatcgtgc catcattgat tggtgccatc
    10981 attttagtac taatcgtatc attctttgct cgaaaactgt aaaaagactt ttaaaaagag
    11041 gtcgtgaaaa agctgttctt catcaagtaa taagaacagc caaacaaaaa tagcttctca
    11101 tatttttcat ttggttgggc atattatcga agatgcaagc ttttgaacac tgtttatacg
    11161 gaataagagg ctgtgacaag acttttgtca cagcctctta tttatgcggg caaattccca
    11221 attagatttt taaaagtttt tccttaaatg tgtctttatt gatacagaga agcttcttta
    11281 ataaaacaat gcctccctaa attttcttga gataggaaag gtgaacatac aaaataatac
    11341 gaccactttg aaaacccaga tgatccacaa atggatcgta tgatactgat gaagtaaaaa
    11401 gcaatggatc agaaactcaa agagattgaa aaagacaagg aagaaataat atctcttttt
    11461 tactcttgtc tgttttagcc aataatagct cgtaaagtaa tctagacaaa caaatagaag
    11521 taaacaaagt ttcgtaagag cagcaatcgt atcatgttgc tctgctagac gattgacgag
    11581 caggaaaaat gttggtaatg gaatatagat cacgagtaat gaaagaaaaa agtaaacaat
    11641 aaatggcata ctattcctcc taattgtgtg agttcgccct cattttaaaa taacgatatt
    11701 tgtttgtgaa gctttttcgt gtcgtaacaa atgttatcta atgtattttt aaataaataa
    11761 tcatta
//
LOCUS       DASJTC010000085        11635 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.086, whole genome shotgun sequence.
ACCESSION   DASJTC010000085 DASJTC010000000
VERSION     DASJTC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.086"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(105..629)
                     /locus_tag="U4B19_002183"
     CDS             complement(105..629)
                     /locus_tag="U4B19_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2077906.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U4B19_002184"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U4B19_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEN2077907.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U4B19_002185"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="U4B19_002185"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEN2077908.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="U4B19_002186"
     CDS             complement(2351..3031)
                     /locus_tag="U4B19_002186"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEN2077909.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="U4B19_002187"
     CDS             3157..4026
                     /locus_tag="U4B19_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2077910.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="U4B19_002188"
     CDS             4052..4567
                     /locus_tag="U4B19_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077911.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="U4B19_002189"
     CDS             complement(4614..5207)
                     /locus_tag="U4B19_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2077912.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="U4B19_002190"
     CDS             complement(5232..5411)
                     /locus_tag="U4B19_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077913.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="U4B19_002191"
     CDS             complement(5488..5997)
                     /locus_tag="U4B19_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077914.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="U4B19_002192"
     CDS             complement(6093..6473)
                     /locus_tag="U4B19_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2077915.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="U4B19_002193"
     CDS             6803..7552
                     /locus_tag="U4B19_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEN2077916.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="U4B19_002194"
     CDS             complement(7736..9013)
                     /locus_tag="U4B19_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077917.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="U4B19_002195"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="U4B19_002195"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEN2077918.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="U4B19_002196"
     CDS             complement(9935..10879)
                     /locus_tag="U4B19_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEN2077919.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="U4B19_002197"
     CDS             complement(10881..11567)
                     /locus_tag="U4B19_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEN2077920.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3321 a         2464 c         2046 g         3804 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaataacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgt
//
LOCUS       DASJTC010000086        11608 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.087, whole genome shotgun sequence.
ACCESSION   DASJTC010000086 DASJTC010000000
VERSION     DASJTC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.087"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U4B19_002198"
     CDS             complement(86..265)
                     /gene="rpmF"
                     /locus_tag="U4B19_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEN2077921.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(323..892)
                     /locus_tag="U4B19_002199"
     CDS             complement(323..892)
                     /locus_tag="U4B19_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEN2077922.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1078..1980
                     /locus_tag="U4B19_002200"
     CDS             1078..1980
                     /locus_tag="U4B19_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2077923.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U4B19_002201"
     CDS             complement(2015..3799)
                     /gene="pyk"
                     /locus_tag="U4B19_002201"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEN2077924.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U4B19_002202"
     CDS             complement(3873..4835)
                     /gene="pfkA"
                     /locus_tag="U4B19_002202"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEN2077925.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="U4B19_002203"
     CDS             complement(5003..8317)
                     /gene="dnaE"
                     /locus_tag="U4B19_002203"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEN2077926.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8548..8733
                     /locus_tag="U4B19_002204"
     CDS             8548..8733
                     /locus_tag="U4B19_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEN2077927.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8795..9991)
                     /locus_tag="U4B19_002205"
     CDS             complement(8795..9991)
                     /locus_tag="U4B19_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEN2077928.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10161..11597)
                     /locus_tag="U4B19_002206"
     CDS             complement(10161..11597)
                     /locus_tag="U4B19_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEN2077929.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
BASE COUNT         3421 a         2478 c         2040 g         3669 t
ORIGIN      
        1 tattttttat ttaccgacta caaaaaaata aacctcaagg ctccatggtt agccttgagg
       61 ttttacattt tctaattaaa atgtattaag cttcttttga cattacgtcc ttaccatcat
      121 agtaaccaca atttgcacat acgtgatggc tttttttcat ttcaccacag tttggacatt
      181 cgtttaaccc tttgattgtc aatttgtagt gtgtacgacg ttttgctttt ttagcttttg
      241 atgttcttct agctggtact gccattttgc tacacctcct tgtaaagtct tgtatctaaa
      301 ttagacacaa tatattccaa tcttacttgt tatcgtcgtc ttttgattta gaatcaaaaa
      361 gttcagatag ttttgcaaga cgaggatcaa ttttattctc ggcttcttcc atttttctgc
      421 gctggtattc ttcttcggaa acaacttccc aatcattgcc ttttggaagt tctgttgctg
      481 cttcctcttc ttttgtcaat acccgcattg gaatcgccaa taagatgttg tcttcaatcg
      541 actctgctaa attcaataat ggaccttcaa ttagcaaaat ctcatctgca gacatttgtt
      601 catctctact ttggaattgt tcttctgtca tgaatacttc gtctacagaa aaatccatgg
      661 gcagttcaac aggttctagt gaacgtgttg aaggaactgt caaagtcgtc tgcacagtgt
      721 aatgaacgat atagcctttc ttatcaactg agaccaaacc ttcgacttcc actggcgcaa
      781 catcaagtat taaattgtct cgcttcatga gagcggcttt caagtcgagc gtctcatgaa
      841 aagttaacgg tgtctcctgg tattttctca attccaataa agaccatttc atttttcatc
      901 actcctaagc aacaaaagca attatacagg gcaaaaagca tgttgtcaat gaaattttct
      961 tgacaccttg cagttcataa aaaaacagaa aaatctttat tagaaagcct ctgtttgcta
     1021 tcatcctcag aatcatttac aataatcatt attccattaa aggagcggat gatcttcatg
     1081 aacacgaaag atatcgaata ctatgtccaa atcgtcaaac aaaaaaattt tacgaaagtg
     1141 gctaattatt tccgtgtgag ccaacctact attacatatg cattacaacg tcttgaaaaa
     1201 gagtttgaca ccgtacttat tacaagagat cgatcgcatc acgaattaat cgtcacgcct
     1261 tctggaaagc agctgtttct ccatgcacaa accattctcc aagagatttc tactgcaaga
     1321 gctgaaatca ctcagttgca aaagaaaaag cttcgttttg gcatgcctcc tattatcggc
     1381 aatgaatact ttcctaaact gtcaagttac tttatcaagc atgaattgat gaatcaaatt
     1441 gagattaccg atggaggctc tcgggactta tacgggatga tccgacaagg gaggatcgat
     1501 ttagctgtat taggctcaac acagccaatc gtggatcaat atttagaaac agagttactt
     1561 ttggacaaac gtttcatgat cgtcgtgcca cctacacatc ctctttccaa gcaggatact
     1621 gtctctttca aagatatagc agatgaacag tttgtactat tgaacgaaca ttatgtccac
     1681 cccgcagcat tcactaagct ttctaaacaa acacatactc atccaaaagt tgtttatcaa
     1741 agcaatgact tgacgatttt aaagagtatg gtccgagaaa atgtgggtat cggttttttg
     1801 gcagagattg ctgtccatcc tgaagatcat ttagtttgcc tccctctttc agatgaagtt
     1861 cagcctcgct tcttgatttc attggcaaaa cgaacacagc agatcgtcac gcctcttcaa
     1921 gaacaagtag tccaagttat ccatgaattc gttacagcac aaaaaaacag ctcttcgtaa
     1981 aagaagagct gtttttattt tttattaagc taaattagat agctgttgtt tcgccacggt
     2041 aaacgatgcc gcgacgtgaa tcaacggtaa tcaattcacc ttctgtaatt gttgttgttg
     2101 cgcctttagc accaacgatt acaggaatat cttgtgcaat tcctacaact gcagcgtgag
     2161 aagtcaatcc gccttcttca acgataacag cagctgcttt ttcgattgca ggcatatatt
     2221 ctttgtcagt tgatggtaca actagaacca ttccgtcttt tgcttttttt acagcttctt
     2281 cagcagatga agcaataact gcatttgcaa cgacagattt ttgtccaacg ccttgagctt
     2341 ctaataattt agaaccaatc atttgaattt tcatcacgtt agttgttccg cgttcaccaa
     2401 ctggtacacc agcagtgatc aagattaaat cgccttctga tgcgaaacct aattcaacag
     2461 cttttttagc tgctagttca aacatttcat cagttgttga tggtttttca gcaactgttg
     2521 ggtaaacgcc ccagtttaac atcaaaccac gttttgtgcg ttcgtcaaaa gtaacagcca
     2581 agatatctgc atctggacga tattttgaaa tcatttttgc agtgtatcct gattcagttg
     2641 cagcaacgat tgttttcacg cctagatttt ttgcagcacg agctacagat aaaccgattg
     2701 tttcagttac atctgtttta tcgaattcgt tcaattggaa tgtacctaat tctgataatg
     2761 cattttcagc ttcttggtcg atacgtgcca ttgttgaaac agcttcaact gggtagtccc
     2821 cgtttgcaga ttcaccagat agcattgttg catctgtgcc atcaaataca gcgttagcaa
     2881 cgtcagaagc ttccgcacgt gttggacgtg ggttttcttg cattgattct aacatttgtg
     2941 tagcagtgat aactgggata ccagcagcat tacatttttt gataatgcgt ttttgaacca
     3001 ttggtactag ttcagctggg atttcaacac ccatatctcc gcgggcaatc ataacaccgt
     3061 cagaaacttt gatgatttcg tcaatgttgt cgataccttc ttgagattcg attttaggga
     3121 agatttgaac atgagtcatg tctttttctt ctaagatttc gcggatatct agaacgtctt
     3181 gtgctttacg tacaaaactt gcagcgataa agtcgatatc gttgtctaaa ccaaaacgaa
     3241 tatcgtcagc atcttttgga gtaatacctg gcaaactaat agaaacgcct ggtgcattta
     3301 cccctttacg tgatcctagc atacctgcat ttttaacttc tacgactaat tcacggtttg
     3361 cttcgtcttt ttcaatgatt tgcatatcga tcaaaccatc atcaaataaa atgtgtccgc
     3421 caacgtgaac atcgtcaaat aatcctgggt aagtaaccgc gattttttct tttgaaccta
     3481 catgttcagg atccatagag atgcgtgttt gatctcctac ttcaaatttg atgtatcctg
     3541 cacgaccaaa atcagcttct gttgtgcctt gaacagttgt acggatttca gctccttttg
     3601 tatctaaaag aataccgaca tcttttcctg tctttttaac tgcttcacgg accatagtca
     3661 tacgtgataa ttgttcttca tggtcaccat gagaaaaatt aaagcggaag acatttgccc
     3721 cagattcaat caattgagaa ataatttcta ctgagttact tgctggtcct aatgtactaa
     3781 cgattttggt ttttttcatt taaaaaactc tcccattcaa ttttgtttat ttaaagctgt
     3841 acacagctta aatagcttgg tttctttcac tcttaaaatg aaagtgaatt atttagatca
     3901 tatagtgata agtctggttt atgtttgttg ttttcaagtg tatcaatgat gtcagacgca
     3961 acgatttcgt tgtcatggat accgacacat aatccacctt ttccttcttg aagcaattcg
     4021 acagcgtaag caccgaattt acttgccaac acgcggtcgc gtgcgcttgg tgctccacca
     4081 cggacaacgt gtcccaagat agatacacgt gtatgatagt caccaaattc tgaaagtttc
     4141 tcagcgaatt catttccgcc cattacacct tcagccaaga tgatcaagca atgtttcttc
     4201 ccgcgatcgc ggccttcttg gattttttta gcaactgaag ccatatcaaa atcatgttct
     4261 gggatgatga tttcatccgc tccgccagcc acacctgacc ataaagcgat gtctcctgca
     4321 ttgcgtccca ttacttcgat aacgaatgta cgaacatgag aagtcgctgt gtcacgaata
     4381 cggtcaattg attctagtac tgtattgatt gctgtatcaa aaccgattgt gaaatcagtc
     4441 ccaggaatat cattatcgat tgttccagga atacctactg ctgggaaacc gcgttttgtc
     4501 aaagccattg cgccatgata agagccgtct cctccgataa ccactagacc ttcgattccg
     4561 aattttttca attgttcgat tccttttaat tgaccttctt cagttgcaaa ttctggataa
     4621 cgtgcagaat acaagaatgt accaccacgt tggatcttgt cacctacatc tgccacatca
     4681 agacgacgaa tatctccagc aactaaaccg gcaaaaccat aattgatccc gtatacttcc
     4741 agtccttcgt aaataccttt acgaacaacg gcacgaacag ctgcattcat tccaggtgcg
     4801 tctccgccac tggtcaaaat tccgatgcgt ttcataataa acattcacct cattaaaata
     4861 ctttatccta agtatgactt aggtttacaa ccatttttgc gttaaccacg actaacattc
     4921 taacattatt tttctcactt gtcttgattt acggataaaa aaatgaaaaa aaatgaaagc
     4981 aagtagtatt ttttaccgta agttatttga aaaccacatt atcttcgcca agcagataaa
     5041 taagttgccc ctttatactc ttgctgtcat ccacccaata agcatctgac agaactactt
     5101 ttcgttgcgt tttttcgtga tacataacta ctggtatata tcctgggtgt ttctgaaaaa
     5161 tcacttgtaa ttgtttcatt atctcgggat cttcccgttc ttctgtaata cgcaaatagc
     5221 aagtttgtga aggatactgg gcttctaact ggttaggatc agcaaagctt tctccaatta
     5281 gttgcagttc ctgattatat ttgctgattt cgctccgccc ttgaacaaag atcactcgat
     5341 caacggcaaa caaattacga tatttacgat aaagagttgg aaaaactgtg acagaaattt
     5401 ctccactact gtcggttcct tcaacaaagg ccatcagttc tccttttttt gtccgaattt
     5461 ctctaacttc cttgatcatc actaataaat tgactggttg attagggacg atatctgtaa
     5521 tcagctgaac ttgtttagct aaacgaagac gatcaaaccc ttctgcagga tgacctgaca
     5581 aataaatacc taagtactcc tcttccagct tcaagcgttc ttctagtgta tagtcagcga
     5641 cttcttcttc ttttaatgcc atgattccca gcaagtcaag acttccacca ctgtaaacga
     5701 tattttggat ttttccttcg agatcaagca tcaattgttt cctgttagga gataattcat
     5761 caaaaactcc tactgctgtc agtgattgca gtaactctaa tttcagccaa cgcttatcga
     5821 tccgtatgag gaattgatcc actgactgga acgggccatt ttgttttctt tcgtttaata
     5881 tatcttctac aaaatcacgc cgaatacctt ttatcgctgt cagtccgaat ctgatttctt
     5941 ccttattgac caactcaaaa ctggaattac tttgattaat ggacggcgag gaaagtttga
     6001 gtttattttt acgtgcttct gcaatatatt ctttcaattt tgatgtgtta tgccgaacag
     6061 aattcatcag gctggtaaag aaagcacctg gatgatgggc ttttagatac gccatttgga
     6121 atcccacgaa tgaataagca aatgcatgtg accgattgaa tccatagttt gcaaattttt
     6181 cgatatattc atatacggtc gctgctgttt tttccgaata tccctgttgt aatgctcctg
     6241 atacaaaatg cgacttttct tcatctaata cgtctttttt ctttttactc actgcccgtc
     6301 gcagaatatc tgactgccct aatgtaaatc cagccatagt cgaggcgatc tgcatgattt
     6361 gttcctgata gacgatcact ccatatgtgt ttttcaaaat cgggatcagt gaatcatctg
     6421 ggtagcgaat ggcttctttt cctttcttcc tggcaatgaa ggtatctata ttttgcatag
     6481 ggcctggtcg atacaatgca ttgactgcgg caatatcttc gatgttttct ggatctaatc
     6541 ttcgtaaaac gttccggata ccagcagatt caaattgaaa tactcctgag gtttcgcctc
     6601 gttgaaacaa gagcagcgtt tttgcatcat ttagcgggat ctcttttaat cggatctcat
     6661 acccttctac tcgcttaaca gctttcaaag taaaatcaat aatcgataag ttgcgtagtc
     6721 ctaaaaagtc cattttcaaa agaccgattg cttctacctc attcatcgta tactgggtca
     6781 gaaaagcttc atcagaaccg cgttggagcg gaactagttc aagtagattc ttatcgctga
     6841 taactacacc tgctgcgtga gtcgaaacat gtcttggcaa tccttctaaa atcactgcag
     6901 ttttgaataa aagttggttt ttttcgcttg cctgaactaa ttctactaat tgatgcgaag
     6961 ttttataagc ttgggtcaat gtgattttca attgattggg tattgctttt gaccagcggt
     7021 ttgcttcact ttgcgataaa ccaaacactc tagctgcatc tctaagaacc atttttgctg
     7081 ccattgtgcc aaatgttgcg atttgtgcca tatgatattg tccatacttt tctcgaacat
     7141 aattcaatac ttcttcacgt cgattgtcag gaatatccaa atcgatatct ggcatcgtaa
     7201 agcgttcggg gtttaaaaag cgttcgaaca gcaaatcata ttctaatgga tccacatctg
     7261 tgatttctaa cacataggct actaatgaac ccgcagcaga accacgacct gcaccggtaa
     7321 cgattttgtt tctatgagca aaagccatta catcccatac gatcaagaaa taatcatcga
     7381 atcccatttg atggatgaca gataactcgt agtccaaacg ttcttggtat tcctttgtat
     7441 agtcagtgat tcggcgttcc acttgcgaat agcataactc tcttaaatat tctccagctt
     7501 gttttccatt aggaactgga taatgcggaa gtaatttctg atggaactgg aattcaaatg
     7561 aaatcgattc agctaaacgc tgggcgccta ttacagcttg ggaaagaccc gctttttcga
     7621 atcgtttgat catttcttgt tctgaaagaa gatagacatt tgctgttagt ggtaaagatg
     7681 cagataaatt ttctaaaggt atttggcttc cctctttgat atgtttcatc acttcaagtg
     7741 caaaaagttc ttcttgttgt aaactagcga tgggatgtaa tgcacaaagc gaataatttt
     7801 cttttatcac atactgatac cattcaggca attgatcagg cgtgttttct gtcaagccga
     7861 tataaaaatt tctagtaaaa acattttcaa gtaaaaactg ccaatgaccg aaaaagttat
     7921 cctctttatt gaaagcctca gaaacagaat gatttgccgg aacaacaaac gctagatggt
     7981 cggtttctgt catttctgca agttccaatt tttcttggat catttttcgg ctggaaagtt
     8041 tcatcagctg ctgataacct atttcatctt ttgcgtacgc aagcaagcca taattttttt
     8101 cttcataagt ataactaaga tgtaatccaa tgattggctg tactccttct tctacacaaa
     8161 tacgatagaa ttcaggtgca ccgtataaga catctttatc cgttatggct aatgaagtgt
     8221 agccttgttt ttttgcttct tgtacatatt ctcgaatacg tatcgtactt tgtaaaagag
     8281 aataagaagt aattgtataa agttgtggga aaaacatctt gcattccgcc ttttttttcg
     8341 ttaaaattca ctagcgttaa aaggtttgat ccttttactt tcattaagat agcaggcgct
     8401 gaagttgatt attccacttc atttgcttgc ttgtaatctg taggaatgag ttctttcgca
     8461 agaactcaaa ttttattgag atttggtctc gtttctttac attcaaagaa aatctggtaa
     8521 aattaagata gaaaagaggt gttctttatg cgatatatcg ttacattatt ctgggctgtc
     8581 gttctaggtc aagtagtcgg ttatattggt gctgcattaa caagcggaac atatgatttt
     8641 actttgacaa caatcatttc attcattgcg ggtgtcatta tccttttgat tggtgcagtt
     8701 gcgccaagaa aagaaacctc tgcccattcg taaaaaaaca gccaagagct tgagatttct
     8761 caagctcttt tttattttct actcctcacc accattataa tctgctgcga agaactttac
     8821 gaaagttgaa taaagaaaga agaatatttg acagaacaaa caaacttgtc acaataaaaa
     8881 cagcactgta attataagca tgcgcgactg tcgatcctag catgggacct aaaacttgtc
     8941 caaagttgct aaacatctga ttgtagctat agattcggct gactccttct tgaggcgtaa
     9001 tcttactgat cagtgtattg acagaaggca tcaaagcgcc agttgaaaag cctaaaataa
     9061 agcgaagtat acctaattgg agcggtgtag tgacgaatgc cataggtaaa taacagcaaa
     9121 atgataaaat gagtcctcca attagaactt tttgattccc aatcttatcc cctagttttc
     9181 ctagtttcgg tgaagaaaag acagcggaaa caccagctat cgaaacaatc aagccgctga
     9241 caaaaaggat attttctgta cttccgctca attcacgaat atataaggtc aaaatagggc
     9301 tgatcgtagt gattcctatc tgtaaaatca aggtcgtaat gaagagaccg atcaaaatag
     9361 agagatgatc catttttgaa aaaatttctt tggtgcttat cagatctttt ttctcgattg
     9421 gatggaaatc ttctttgact aagaaaatgg tcaataccgt tgtgatcatc aacaaagccc
     9481 ctgtgatcaa gaatacattt tccataccaa accactgagc cagtgcaccg cccattgaag
     9541 gtccgatcag atttccagca atcgctccgg tagacaatgt tcctaacgcc catccgctct
     9601 tttctctagg tgcctgtgaa gcaatcatcg ctgtggcatt tggaatatat ccagacaaaa
     9661 tcccattcca aaaccgcatg atcaaaagcc aatacgcgtt tggtacaaaa gccaaagatc
     9721 ccattgttaa tgtcatccct gctgctgcac ggatcatcat gagttttcgg ccttttcgat
     9781 cagctagatt tccccagatt ggtgcaacca cggcagatgc aaaagcagtc acagagattg
     9841 ctagtcccga aaacaattcg atttgatctt ttggtgttcc tagttgctca acataaactg
     9901 gtatgaatgg cataactaaa ctaatacttg caccagtgaa aaaacagcca atccacgcaa
     9961 tcactaaatt ttttctccaa ttaatctcca tctatcatcc ctctttcgtc tcttttatca
    10021 tacctatttt cgaataaaca agcaacgatc tcgccagtaa aaagagaaaa atcccgcaaa
    10081 ggtcatttta tatgtaacgt tctttttatg taaaacaaaa agagatcaga acaaatactg
    10141 ttccaatccc ttctctttag ttaaattgtt gctagaatga tgaagttgat aatgaataaa
    10201 atatcaacga tccacaaagc aacagaaact tgattgtatt ttcctttgat tactttcaca
    10261 attgtataga aaataaatcc tgctgcaatc ccataggaaa tgctatagca caatcccata
    10321 aagatcgatg caaagaaggc cggtaaagct tcttccatat ctagccattt gatgtctgca
    10381 aaagaagcca tcatcattac tccaactaaa atcaatgctg gcgcagttgc ttgatttggt
    10441 acaatggcga tcagtggaga gaataaacta cttaatgcga atagcactgc cactactact
    10501 gaagtcaaac cagtacgtcc accggccccg atcccagctg cagattcgac ataagttgtt
    10561 gtattcgatg taccaaagat agcccccaca gaagtagcga tcgcatcggc aaatagtgct
    10621 ttatccattt ttgttttgaa cccacgacca tcttccaacg cgatctcgtc ttcttttgag
    10681 aaaatacctg tgcggcgtcc tgtaccgata aatgttccga tcgtatcgaa agtatcagat
    10741 aaactgaatg cgatgatcgt catcaatact tgaggaatct tggaagaatc actgaataaa
    10801 gattgcatgc cttcagaacc aaatgctgca ccgaaagttg ttcctaattc tttaaatgaa
    10861 ttacctaacg aattcgtttg ccaatcaatg gaacttaaat caacaacacc cataaaaata
    10921 ccaaggactg tggtcgctac gataccgata agaatagccc cacggacatt ttttacaact
    10981 aaaatggctg tcagtaccaa accaatcaca gccaataaga taggagcgtt gtcaaagttg
    11041 gctaacgcag ggacgattcc accatttatc gtaacatttg tcgcttttcc gccttcaacg
    11101 acagagctcg aaatagctga ctggtctgct gaaaaagata agaatcctgc atttttgatt
    11161 ccgacatacg caacgaaaat accgatcccg ccaccaatcg catgttgaag actttcggga
    11221 atcgcccgaa taatcatttt tcgaatattt gtgacagtaa taaaaatatt gattaatccg
    11281 caaataaaca ccattgccaa ggcttgttgc caagaataac caagcccaaa aacgaccgta
    11341 aaagtgaaaa atgcgttcaa tcccatacca ggtgcctgag catatggtac atttgcgaac
    11401 aatcccatta ccagcgtacc aataatcgat gcaatgattg tcgctagaaa tactgcttgg
    11461 aagggcatac ctgatgctga caaaatcgtc ggatttacga ataagatata actcatggca
    11521 aagaatgtcg ttactcccgc cattatttca gttgatacat ttgtattgtt tttctttaaa
    11581 ccgaaaaact tttccataaa aagttcca
//
LOCUS       DASJTC010000087        11532 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.088, whole genome shotgun sequence.
ACCESSION   DASJTC010000087 DASJTC010000000
VERSION     DASJTC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.088"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(95..2170)
                     /locus_tag="U4B19_002207"
     CDS             complement(95..2170)
                     /locus_tag="U4B19_002207"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEN2077930.1"
                     /translation="MAKDLLLEIGLEEIPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFTHAKQYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2172..3089)
                     /gene="glyQ"
                     /locus_tag="U4B19_002208"
     CDS             complement(2172..3089)
                     /gene="glyQ"
                     /locus_tag="U4B19_002208"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEN2077931.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3473..4285)
                     /gene="recO"
                     /locus_tag="U4B19_002209"
     CDS             complement(3473..4285)
                     /gene="recO"
                     /locus_tag="U4B19_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEN2077932.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4428..5327)
                     /gene="era"
                     /locus_tag="U4B19_002210"
     CDS             complement(4428..5327)
                     /gene="era"
                     /locus_tag="U4B19_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEN2077933.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5342..5737)
                     /locus_tag="U4B19_002211"
     CDS             complement(5342..5737)
                     /locus_tag="U4B19_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEN2077934.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(5721..6197)
                     /gene="ybeY"
                     /locus_tag="U4B19_002212"
     CDS             complement(5721..6197)
                     /gene="ybeY"
                     /locus_tag="U4B19_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEN2077935.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6214..8406)
                     /locus_tag="U4B19_002213"
     CDS             complement(6214..8406)
                     /locus_tag="U4B19_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEN2077936.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVVFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8430..9401)
                     /locus_tag="U4B19_002214"
     CDS             complement(8430..9401)
                     /locus_tag="U4B19_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEN2077937.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10107..11486
                     /gene="brnQ"
                     /locus_tag="U4B19_002215"
     CDS             10107..11486
                     /gene="brnQ"
                     /locus_tag="U4B19_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN2077938.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHYVPFAALGLGWVVPTLLGYAGSLLFYYVYRVSGYK
                     QEASELPEE"
BASE COUNT         3108 a         2421 c         2119 g         3884 t
ORIGIN      
        1 attgaattaa taatagttat ttatatttat aacaaataaa ccagagaaat agcctatttt
       61 gtgcctgttc tctggtttat ttacatatta gaaattattt tgtgatgatt tgatctaaac
      121 ttgctagtga caaagccatg gcagcaattt gacttaattg cgttaatcgg ttcttacgta
      181 ctgctggttc ttctgccatg accatcgtct gatcaaaata attttcgatc agtgggcgaa
      241 ggcttacaag ctgatcataa ttttcgttga tcgtttggtc agcaaagctt gttttcattg
      301 cctctactgc tttgtgcagt tctttctccg cttcgttttc gaacagttca gggtctactt
      361 ctgattgttt ttcaagctcc gcttttttcg ctaagttcac gacacgtgtc aaagcttcca
      421 tcgatggttt gaagtcttca tctgcaagat gctgcttgaa caattgtgct gctgcaaata
      481 cttttgtcag atctttttgt tctgcattca aaacagcttc gatgacatca tgacggatat
      541 tcttcgttgt caataattgg cgcagacggc ctttgataaa gtcgatcacc tgttgttgtc
      601 ccgctgacaa gccaatgcca aaacgatccg tatctttact gataacttca ctaatcgttt
      661 cttgcagaac agacaaagga aaggcccatt ttttcgattc gatgattcga acgacaccat
      721 atgcttgacg gcgcaaagca tatggatcgt ttgagccact tgggatcatc ccaactgcaa
      781 agaaagaaaa gatactatct aatttatcag caatcgctaa aacagaacct actgtagtct
      841 caggtaaatc tccttcactg gatataggca tgtaatgttc acggatcgct gttgctacgg
      901 ctggtttctc gcctttaagc agagcatatt tttcgcccat gatcccttgt agttcaggga
      961 attctcctac catgttggtt actagatcaa atttatagat ttcagcagcg cgttgcagat
     1021 ccatcaattc ttcctcagat agtcctactt gttcgcctat gatgcgagcg atgacttgta
     1081 cccgttgcat tttctcatag atgctaccga ttttttcatg gaaagtaaca gtttttaatt
     1141 tttcaacgta ttggtcgatt gaagctttct tgtcttcatt ataaaagaat tccgcatctt
     1201 ctaaacgagc agtcaatact ttttgattgc ctcgtaccac attttccaat tttacccgat
     1261 cgccattacg agcagaaata aaatgaggaa gcaacatacc ttgatcgttc cgtacttcga
     1321 aataacgttg atgttctttc atagaagtga ctagtacttc ttcaggaaca gatagatatt
     1381 tttcttcgaa gtcgccagaa aagacagttg gatattcgac taaattgttg acttcttcta
     1441 gtaaatcttc atctagatca atgatccagt gatttttttc tgctaattcg tttgcttgtg
     1501 tgatgatcat ttcttttctt ttttgcggtg aagcaataac aaattgttca ttcaattttt
     1561 cttcgtattg ttttgcatgc gtaaatgtca catcttcgcc taagaaacga tgacctcttg
     1621 aagctctgtc tgttttgaca tccaagatct caaacgggat tatttcgtca tctaacaatg
     1681 ccacgatcca gtgaataggg cgaatatatt caaaatcgta ttctgcccag tgcatcgtta
     1741 ctgggaaagt gaggctttta atgacatctg ctaaacctgt caatacttct tttgcttctt
     1801 tgccgtgagt gtatttcgtt acatagacat attccacgcc ttttaattct ctaaaagtga
     1861 tagcatctgt cgtcagacct tgtccgcgga caaatccttc agctgctttc gtccagtttc
     1921 catctgcgtc caaagcaatt tttttggctg gtcctttgac ttcttcttcg ctgtctgctt
     1981 gacgttctgg aatgtttgtt actttgattg ctaagcggcg cggtgtagaa aacgtttcga
     2041 tcgtatcata agtcaattgg ttttcatcta aaaattttgc tgttttttct tctaattgtt
     2101 tcatgcttgg tgtgactaca tgagcaggta tttcttccag cccgatttca agtaataagt
     2161 cttttgccat cttattcagc ctccttcacg ttttctggat ttgctgtgtt tttattcaat
     2221 aaagggaatc ctaatttttc gcgttctgcc acaaagattt tggctacagc acgagccatg
     2281 ttgcggatac gggaaagata acctgcacgc tctgttacgg atactgcacc gcgagcatct
     2341 aataaattga atgtatggct acatttcaaa atataatcat acgctggatg aacaagattt
     2401 tcatcgatac agcgtttagc ttctttttca aaacgatcaa aattgcttag tagcaggtct
     2461 tggtcgctta cttcaaaaga atatttagaa tgttcatatt ctggttgttt gaagatctca
     2521 ccatatttca cgccatcagc ccattccaga tcataaacac tttcaacttc ttggatataa
     2581 gaagcaagac gttctagtcc ataagtgatc tcagaagtaa ctggtttaca aggtagtcct
     2641 ccgacttgct ggaagtatgt gaattgtgtg atctccatcc catcaagcca tacttcccag
     2701 cccacaccgg cacaacccat cgatgggttc tcccagttgt cttcaacaaa gcgaatatcg
     2761 tgttctaatg gatcaattcc taaaagacgc aagctttcta aataaagctc ttggatattt
     2821 tcaggtgaag gtttcattac gacttgaaat tgatgatgct gatacaaacg gtttgggttt
     2881 tctccataac gcccgtcagc tggtcggcgg gatggctcta cataagcagc attccatggt
     2941 tccggtccga ttgcccgtaa gaatgtatat gggctcatcg tacctgctcc tttttctgta
     3001 tcataagctt gcatcagcat gcagccgttt gatgaccaaa atttttgtaa tgttaaaatc
     3061 atatcttgga cggtcaattt tttactcatc tgttttgccc ctttcaattt ttaagcgttg
     3121 ctgtcaataa gaatacagga aagaaaacga caaaaagtcc ctatgtctga ttacagacat
     3181 agggacgaat aatttcgcgg ttccacccta cttccgaata aagattcggc agcttcattt
     3241 atcgtactcg caagtgcctc ttcaaaaatc gtttgggtcc ggcttccact ctcccgaact
     3301 cgctaaacca cgtgtttttt actgatcttg gtcaatgtac gtattcattt tgcagttttc
     3361 tggtttgtat aatagatcct ctcagtgaac gatctattta atctacggtt aataatagac
     3421 ggaagtccct gtatttgtca aatagttttg atttttttgt tgtatctttc atttattctt
     3481 ctttttcagg tatctctttt tcaggcatat ttttctctgg cgtctttaat acgtgtgccc
     3541 aagacttcat ttgatcaatg aactttttgc tttttagatg aataccgaca tattcatcat
     3601 acaactggtc aatgaggtca cggattgcct gcttcgtttc ttctttcaag tggattccac
     3661 tgatcttatc ataagaaata gccgaaaaca ttcgaacgaa ataaacagcg cgaggatctg
     3721 catgataacg gtgttcgtcc attggccagt gtttctcgca tatcacaccg ccatatttgg
     3781 aagaatagtc aaattttcct tttgtttccc cgcatacgac acagtgcgtc caaattggcg
     3841 cgattccgaa gcgttgcagt atttgcactt caaagatatt cgtgatgatt tctgcatcat
     3901 ctccttggtc catccgctgc agagcttgtt gcgtaaattg gaataaatgc gggtcatatt
     3961 gatgatcttc gattgctgca tctactagat ttagcaaata cgtaccataa ccgctcaaaa
     4021 agatatcttc ttggatgttc cgaaacgcat ggacgtcttt gacactgctt aaaaacgaca
     4081 aaccttcact tcgaaaatta ccgatataga ctgcttcggt aaaggggagc aaagccggag
     4141 ctaatggctg attttgccga tggattcctt tgatataaaa catcattttg ccagaagatt
     4201 ccgtaaagat tttgactaat ttatcttttt ctttgtaatt acggctagat aaaatcaagc
     4261 ctttcgattc tgcaagctgg ctcatattct ccctcctaaa aaataaatct ggaaaagcag
     4321 attctgccga tccagattat ttatgtttcg atcttccgct gtttttctca acgtacggat
     4381 ttttatccag tttatttcta tacctaacct ataagaagtc cagaatatca gtactcgtct
     4441 tttcggtagc cgaagtcttg cagatagact tttttgtctc gccagtcttt ttggactttg
     4501 acccaaagtt ctagaaatac tttgtctcct aaaagatttt cgatatcctt tcttgctttc
     4561 gttcctactt gcttcagcat ttttccgcct ttgccgataa tgattccttt ttggctatct
     4621 ctttcgacga tgatcgtggc ctggatatgg attttatcat tctcgtttcg cttcatcgaa
     4681 tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg ttaaaaacaa gactttctca
     4741 cggatcaatt cagaaacgat gaagtattca ggatgatcag tcacttgatc atctggaaaa
     4801 tattgtgggc cctctggcat ttcactgact aatacatcca ttaagcgttc gacattgttt
     4861 ccttctgtag cagagatcgg aatcacttgt gcaaaaggca tctgcgtcga ataatcttcg
     4921 atgattccca gcaactcgtc tggatggatc ttatcgattt tattgataat cagataaaca
     4981 ggagaatgca cgttttttag acgttcaata ataaaatcgt ctccccggcc acgtttttga
     5041 tcagcactga tcataaataa gactgcatcc acttctttca acgcactgta agctgcttct
     5101 accataaaat ctcctagacg atgctttggt ttatggattc ctggtgtatc gataaaaatc
     5161 agctgagctt ctggtgtagt atatactcct tggattttat ttctagttgt ctgtgccttg
     5221 tcactcataa tggcaatttt ttgacccacg attcggttca ataatgtgga ttttcctaca
     5281 ttcgggcggc cgacgattgc gacaaaccct gatttatgtt ctggcatata cgttctcctt
     5341 tttaattaaa caacaaatga tatattttag ggacaaataa aatcaaccca atgatgacag
     5401 caaaacaagc ggtcactaaa actgctccag ctgccatatc tttgattttt ttcccaatcg
     5461 gatgaaaatg aaaatccgta aacatgtcca ctacattttc aaaaacagta ttgatgatct
     5521 ctactatcca aacgagaaaa acagctaaaa gcagccataa ccactctatc cgatcaagct
     5581 ggaaaatcac gcccataaat aaagcaagga taccgaacgc aacatgtttc ctcatattgc
     5641 gttcttcctc aaaaacggtt ttgactcctt ggacagcaaa ttccacagac atcaggaaat
     5701 gtttgttttt ctctacgttt ttatcttttg agtccatagg catccaagat ctctttctgt
     5761 aaaccaaaca tttctttctc atcttctggt gtcatatggt catacccgtt gatatgcaag
     5821 aatccatgta ccgctaaaaa gccaagttcc cgatcatatg aatggccgta agcttctgct
     5881 tgttcagcag cacgttcaat agagatcatg atgtctccta gtacacgagg taatggattc
     5941 tctccatctt caaaaatcac tgggatttcg tcttcgcctt cttcttccag agcaaaactg
     6001 atgacgtctg tcggtgcatc ttttccacgg taatcacgat tgattacttg gattgccgca
     6061 ttgtccatga aagtaacaga catttctgtt tcctctggca gctttaaata agatgctgca
     6121 aattgcagca agtcatcgat ttctttcatt ttctcttctg agacttggtt tgtttcatcc
     6181 ataaaggtaa tatccattat acatcgcttc tttctattct tcttgttctt cctttatctt
     6241 ctcaacttct gttttcattt taggatattt gatccgagaa tggaaggtgc tacttaaacc
     6301 gctgattaat gatttttcca cttttcggat ttctttcaag gtcagtccac tatcgtctaa
     6361 ttgaccatcg gaaatacgct cttcgataag attatggaca aaactttcga ttttttcgct
     6421 cgtcggatga tccattgccc gtactgcagc ctcacaactg tcagcaatat tgacgatccc
     6481 tgcttcacga ctttgcggtt ttggtccagg ataacggaat tgctcttctg ttacttccgg
     6541 attgcgttct ttcgctttga tgtagaaata acgcattagc gtggttccat gatgctgttt
     6601 gcaaatatcg atcaccattt gcggcatatt ataatcttcc aatattttgg ctccatctgt
     6661 cacatgtcca aagataatat gtttactgtc ttctggcaat aagaaattgt gcgggttttc
     6721 cgctcctgaa ggcaagtttt caacgaaaaa gctagcatgt ttgatcttgc cgatatcatg
     6781 ataatagcaa gctactcgag ttagcaaaga acgtccgccg atttctgcta cagcgttagc
     6841 acttagatta gctaccatca tactatgatg ataagtacct ggcgcttctt ccagcaactg
     6901 tttcaataaa ggatgatttg gattgctgag ttcatttaat acaattacac tgtcatcatt
     6961 gaccatcaat tcaatatatg ggtgtagacc gatcgtcagc aagaatgaga agagtgcacc
     7021 agtgaacccg cagacgagca ttaaccaagt cgttgcatca ccaaagctca ttccttgata
     7081 gacgatcaaa atgacatcca tcatcacagg aaaaataatg acccacatgg cagcagataa
     7141 tccttgttct gagatccgtt tgcgtctgac aatcgttgcc atgagcccag agaaaacata
     7201 agacatcaag ataatcgtta gaaagttcgt gccaatcgaa tcataaaaaa tgaaaagtgc
     7261 ggatactgct tggaaaatac cagccatgat ccctgctctt cgattcaaga aaaagcttaa
     7321 aacaagtggt gcaaaagcag cgggataaaa cagcgggata taagtagctt gttccgtttg
     7381 gaataattgg aaaaatttca tcagcactat gctgactgac atagctgtca catagaaaag
     7441 cacgtatttt gttctttctc ctgcattttg gaattgaagt gaattataaa tcagtacgcc
     7501 gatttgaagc aaaacagcta aaaccatggc aattagcgga aaaatcgaag ttgtttgatt
     7561 agtcagtcct agtaattcca attttttcat agctgaagca tcgatttgat taccttcacg
     7621 aacaatgatt tctccttgga agatcatcac tggttgtaca gcttctcgtg cggattgttt
     7681 caattcttct gtctttttct cattcaatac gtcattcact acgatacctt gatccactaa
     7741 gtagcgaatc atttgttgtt gtgctggtgt gacattcaaa tattggattt gctcttctgc
     7801 ttcttgttta aaagcttcca attcactttc acgaacttgt ttgctcatct gttcgtcaat
     7861 gagcttcaga ctctcatccc tcacagtatg aatctcagtt tcagtcattt caaatatcgt
     7921 cgaataaaaa ttgttcggta gtttttgata aaacacaaca ttttctgcat tcacattttc
     7981 aaaatttttc ttcagcgcgg cgatccgctc ttctgtagta ggctttggga tattttcatc
     8041 ttcctttgcc ttatcaatct tttcgtcata agatttgttg atggcatcat tcgtcttatc
     8101 gatcaattca aatagttgct tgatccgatt gttctggtca tctgcaagat ctttttggta
     8161 agtgtattct ggagtgactg cttctgctgc cagttttctt ttttgttcgg tttcttccgt
     8221 gttctcgatc gttttgttcg cacggatact ttcttctgct acttggcctt ctctgaaatc
     8281 attggccttt tgtttgacac tactaaatgt aacgccgcag ataatgataa aaaacaagaa
     8341 cagcaggaaa ggcaatgctg cctttccaag tttttcttgt agtttctgca gtgttttatt
     8401 caacatgtgc ttatcctcct aagcaaagct tattcttgat ggagatctgc cttttcataa
     8461 gctttgatga tttcggcaac tacagggtgc cgaacaacgt cacttgcttc aaaattaaca
     8521 aaatcgattt tcttgatttg ctttaatgtt cgttctgcat gaacaagacc acttgtcgtc
     8581 cctcgaggca agtctatctg acttgtatcc ccattgacaa tcatttttga ttgataaccg
     8641 agacgggtca agaacatttt catctgagcg actgtcgtgt tttgtgcttc gtccaagata
     8701 acgaatgcat catccaatgt tcgcccccgc atgtaagcta aaggtgcgat ttcgattacg
     8761 cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa tttggtataa agcatcataa
     8821 acaggccgca gataaggatc cactttttct tttagatccc cgggaagaaa gcctagattt
     8881 tcacctgctt ccacagcagg ccgcgtcaaa atgatttttt gcacttcacc ttttttcaag
     8941 gcagcgattg ctaatactac tgcaagaaaa gtttttcctg taccggctgg tcctacgcca
     9001 aatacgatat catgtttggc taccgattcg acatatttct tttggccgct gttttttacc
     9061 cgtataggtt ttccattacg gtctttgacg atctcttcct catacatctc aacaaaataa
     9121 tctaatgtac ctttttgccc cattttcaat gccgtcacaa catcgggcgt acttacgtgg
     9181 attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg aggcagccaa ggaaatctgg
     9241 gattgctcac caatgatctg aatgatttct ccacgtgtat ggatggttgt ttccgttgtt
     9301 tcctcaatca gttttatatg cttgtcgtgt gcacctaaaa gcatgctgac atcatcagca
     9361 gccgtcaatc taatctctaa agaactagac tcttccgtca aaaatagaca tccccttttt
     9421 ttcgattcat tctttcctat tttaccatac tcagaagaaa ctatatagca aataagaaaa
     9481 ggttctaaaa gagttgctta accataagta ccaagaaacg ttaaaaaact agccgcttcc
     9541 attctctaaa ggtctcgttt gaataaaaaa agctgatcat gtaagctatg cataagatag
     9601 aggacaagta ttccgtctaa cttagcaagt attctctggg tatttttctt gtttcaaaaa
     9661 aagaaaacaa aatatttgaa atattcttgc atttcaaaaa aaacaggtgt attataacgt
     9721 caacaaataa atatacagca aagcgtcgag gaaaacagta ggctattcag tgatttattc
     9781 ccagtgagtc atgtaagtga gaaatgacaa taaatcgaat agacgaacat catttccgag
     9841 tcaatgtcga aaacgtagat cattgtggct gcctccatta tcaggctagc ggataacttt
     9901 atcgaaaagc gtccgtgaat gaagaagctt ttttaagcga aaaaaggtgg taccgttcta
     9961 gaatctagag cccttttccc aagtggaaaa gggctttttt tatattttaa ggtggtggtt
    10021 ttcatggaat ctgatattag tggtatttct agtttctgca acgtttattt tgttactcaa
    10081 aatgataatt attggaggaa aaatttatgg aagaaacaaa aacttattca tggaagcaat
    10141 tattgatggt cagttcactt atcttcggga tgttcttcgg gtcaggtaat ctgatctttc
    10201 ctgtacactt aggtcaaatg gctggatcaa actggatcag cgctggtgta ggatttgcca
    10261 tctcaggcgc tcttttccca ttattagcta tcttagctgt agtagtgaca aaaagtgatg
    10321 gtctgtatga cttagctaaa cctgtcggaa aatggtatgc tgccttgttc ttgatccttg
    10381 ttcatttaac gatcggacca ttctttggta caccgcggac agcagcaact gcttttgaaa
    10441 tggctgctaa accctttttg cctgaaaaat atgattcatt aggtatgttt ttattctctg
    10501 ctcttttctt tttacttgct tatttcctta ctgtacatcc aacacgtttg gtcaaatatg
    10561 tcggaaagta tttaaactcc ttgtttcttg cattattagc tatcattttt gtcgtttctt
    10621 tattccaacc catggggaat ctaaaccaat ctgctgcaga ttcttatcag acgaacgctt
    10681 tgacaaatgg cgttttagaa ggatacaaca cggttgatgc agtagcttta cttgctttaa
    10741 gtgtcacttt tgtccatgca gttcgtgatc ttggttttaa agataaaaaa gtcacagaat
    10801 taacagcaaa agctggtagc ctgagcgttg cgttagaagt catgatttat tttggtctgg
    10861 tccttctcgg tgcgatgagt ttgaataaga tgtctttatc agaaaatggc ggaactgcac
    10921 tttctcagat cactaatttt tatttaggta acttcggtgc tagcttctta ggggtaatgg
    10981 tcacattagg tgtcttcact actgctatgg gactcgttgt ttcttttgca caagatttcc
    11041 ataaactgtt ccctaaagtc agttatatga catggctgcg gttaacgact tttgtctctt
    11101 ttgtcgttgc gaatgccgga ttggataata tcatccaatg gtctttacca gtattgatgc
    11161 ttttgtatcc attgtcactt gcgttaatct tactttcctt aacagcaaaa ttcttccaaa
    11221 agagtccatt tgtctatcaa gtaacgatgt tcttttctgc cgttccagcc gtattggata
    11281 tgttggcaaa ttcgcctgca ttagtgagtc agcaaactgt cgtagcaagc ttactaggat
    11341 tttatcatca ttatgttcct tttgctgcat taggtcttgg ctgggtcgtc ccaacgttgc
    11401 ttggttacgc tggcagcttg ctcttttatt atgtatatcg cgtatcagga tataaacaag
    11461 aagcaagtga gttaccagaa gaataaccaa atcaaacaca atttaacata agagatatta
    11521 aacaggaaaa aa
//
LOCUS       DASJTC010000088        11264 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.089, whole genome shotgun sequence.
ACCESSION   DASJTC010000088 DASJTC010000000
VERSION     DASJTC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.089"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            226..1485
                     /gene="lysA"
                     /locus_tag="U4B19_002216"
     CDS             226..1485
                     /gene="lysA"
                     /locus_tag="U4B19_002216"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEN2077939.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1472..2272
                     /gene="dapF"
                     /locus_tag="U4B19_002217"
     CDS             1472..2272
                     /gene="dapF"
                     /locus_tag="U4B19_002217"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEN2077940.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2275..3156
                     /locus_tag="U4B19_002218"
     CDS             2275..3156
                     /locus_tag="U4B19_002218"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEN2077941.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3153..3869
                     /locus_tag="U4B19_002219"
     CDS             3153..3869
                     /locus_tag="U4B19_002219"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEN2077942.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3875..4570
                     /gene="dapD"
                     /locus_tag="U4B19_002220"
     CDS             3875..4570
                     /gene="dapD"
                     /locus_tag="U4B19_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEN2077943.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4586..5587
                     /locus_tag="U4B19_002221"
     CDS             4586..5587
                     /locus_tag="U4B19_002221"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEN2077944.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5584..6945
                     /locus_tag="U4B19_002222"
     CDS             5584..6945
                     /locus_tag="U4B19_002222"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEN2077945.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6905..8056
                     /locus_tag="U4B19_002223"
     CDS             6905..8056
                     /locus_tag="U4B19_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEN2077946.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            8031..9200
                     /locus_tag="U4B19_002224"
     CDS             8031..9200
                     /locus_tag="U4B19_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2077947.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9375..10754
                     /locus_tag="U4B19_002225"
     CDS             9375..10754
                     /locus_tag="U4B19_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2077948.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            11035..11264
                     /locus_tag="U4B19_002226"
                     /pseudo
     CDS             11035..11264
                     /locus_tag="U4B19_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3749 a         1958 c         2508 g         3049 t
ORIGIN      
        1 ttcatcacta aatagtgttg aatttatgtt tttatgtata aatagcttct aatagttcgg
       61 gtattttgtt tttataacca tttttattct tatttttaaa aaaatatttc gaaatatact
      121 agacaattca gaaaattttg tttaatatta ttccaataaa attcaaatct ttatcaaatg
      181 aaattccaat aaaaacggaa aaaaagtgaa aagaggaatg aaaaaatgac agaattgata
      241 attggcggag tcacagcaag cacattggca gaaaagtata agacacctct gtatgtgtat
      301 gatgaagaaa agatgagaca aacgatgctg tcattcaagc gaggctttgt atcgaaacgt
      361 tttgatacaa aagtgcttta cgcctctaaa gcattccaaa cgattgagat gctgcattta
      421 gttcgtgaat atggattcgg gctagatgtt gtcagtggtg gggaaatcta taccgctttg
      481 caagctgatt ttcctagaga tactatctac ttccatggta acaacaagac gccagaggaa
      541 ctgatctatg cacttgaaaa tgatttgttg catattgttg ccgataattt gatggaagtt
      601 gaattattag cagaattaag ccaacgctat ttaaaaaaaa tctgtatcat gcttcggttg
      661 aatgtcggag tagaagcaca tactcatgaa tatatcgtca ctgcgcatat tgattcaaaa
      721 ttcggcatga gctatgaaag taaagagtgc caacaagccc ttcagttgat aaaagaaagc
      781 agctatctag agttagaagg atttcatgct catatcgggt cacaaatttt tgatgttact
      841 gcttggttag ctgaaatcga caaattgacg aattacttaa ccgattttga ccaacctctt
      901 tccttgaatc ttggcggagg gtttgggatt cgttatacac aaaatgatca gccgatccca
      961 gtcgaagagg cactaaaaaa actgattgaa tatacggaag aagctttgga aaaacgaaaa
     1021 ctgaccattc gtcaattact gatcgaaccg ggtagaagta ttgttggaga agctggtaca
     1081 acactataca cgattggttt catcaaaaag acaccacata aaaaatatta ctttgtagat
     1141 ggcgggatga cagataatat ccgtccagct ctgtatcagg cagaatacga ttgtgattta
     1201 gcagcaaaac tgtcggaaga aaaaacagag aaagtgacga ttgcaggaaa aatgtgtgaa
     1261 tcaggcgatg tggtaattaa agaagcttat ttgccgaaag cagaatcggg tgatctttta
     1321 gctgtttatt ccacgggagc ttatggatac tcgatgagca gcaactacaa tcgagcgaca
     1381 cgaccagcag ttgtttttgt caataatggc caatcaagac tgattgttcg cagacaagca
     1441 ttttctgatc tactaagggg ggaagtttcc tatgatcctt cataaatata atggctgcgg
     1501 aaatgatttt atcttattag attacgcaga agacacagat tatagtcaat tagccgcttc
     1561 tttatgcaca aaagaaaaat atgatataga cggattgatc gccgtaaaaa tcgatccgtt
     1621 ggaaatgatc tattacaaca aagatggcag ccgcgcgccg atgtgcggaa atggcattcg
     1681 ctgttttgct cgatacgtaa atgatcagaa catgatgaat gaacaggaat tcaacgtaga
     1741 gacattagca ggcacaagaa aaattacggt gttggatccc tcaccttttt attgcagcgt
     1801 agatatggga gtgccagatg attctccgga aaaaatcaaa gctaatcaaa cttatccaat
     1861 aaaaaaccag gaaatagaga tcaatggtga aaaagtaacg atcactagtt tatttttagg
     1921 aacgattcat accgttgttt ttgttgaaaa tgcgcgtgag gaagtctgga gaaaaagagg
     1981 tgaactgatc tgtcatcatc ctttgttcaa agaaaaaacc aatgtcaact ttgtagaagt
     2041 agtcaatgaa caagaattga tcgtccgtac ctatgaaaga ggagtaggtt ggacacttgc
     2101 ttgcggtaca ggttgctgtg cctcttatgt ggttgctaag gaaactggga aaatttcagc
     2161 agatcaggta gttgttcact tggaacaagg agacttgatg atctctggtg aggatgcgat
     2221 acaaatggct ggcccagcag ttcatgaatg gacaagaata ttggaggaat gaagatgata
     2281 aaaggttcga tcgtggcgct gatcacgccg atgaatgaag agggatcagt agattatgct
     2341 ggtttggaaa aattgatcca attccattta gatgaacaaa cagatggatt attagtttta
     2401 ggtacaacag gagaatcttc tacgctcaca caatctgagg aggagcagat ccttcaactg
     2461 acagtaaaga aagtggctgg ccgtgtgccg gtgattgcag gagctggaac gaataatacg
     2521 aaagaaacga ttgaaaaagc gaagcatttt gcttctttgg gggcagacgc acttttggtc
     2581 atcacccctt actacaacaa aacaagtgat gcaggactag ctgctcattt tacagcaatt
     2641 gcagaagcct caccgattcc gcttattttg tataatgttc cttctagaac aggcatgtcg
     2701 attccaatcc atgtattagt aaatttagca gaacatccta atatcattgg tttgaaagaa
     2761 gcctcaggag atatggctta tgtgatggat gccgctcggt tgattggaga agaattcttc
     2821 ctttacagtg gaaatgatga tttgatccta ccagtaatga gtgtaggagg aagcggtgta
     2881 ataagtgtat gggcgaatat ccaacctaaa atagtccatg aattagtaaa agatacgcag
     2941 gacggaagat ggcagcaagc aaaagaaaag caattaaatg cacttgaact gatccatgcc
     3001 ttgttcagtg agaccaatcc tattcctgtc aaagcagcca tgtcattatt agacttacct
     3061 tccggacctc tacgcctgcc tttagtttct ctttcaaaag agaaaaagaa acaattagct
     3121 cagcttttgt taaaagaaag gacggttgaa aaatgaaaat cggactgatt ggcagtggac
     3181 agatgggaaa tagagtcgaa gaagttgcac aagaaagagg agatacggtc cttcttttac
     3241 ggacggcacc taaagaagca actacgcttg cttttacgga actgccagaa ctgcttattg
     3301 atttttctca tccagataat ttagagaaaa tcctttcgta tagcttatca tatcaacttc
     3361 cgttagtcat tggcacgaca ggttatacac aatcacagtt ccaagaaata aaagaacatg
     3421 ccaaacatgt acctgtgatg tatagtgcta atttttcgtt cggcatcatg gtgatgaatc
     3481 aactaatcaa gcaagcagct gcaatgttgc aaggctggca gatcgaactg attgaaaaac
     3541 atcatagtcg taaaaaagat gcaccatctg gtactgcaaa tatgctgctt caaacaatcc
     3601 aagaagtgca aaagctgcag cccgtataca actggcaagg gaaaaaaaga gaagaaaacc
     3661 aaattggtgt acacagtatt cgtgcaggtt ctttacctgg tgaacatgat gtattgtttg
     3721 ctgctactga tgaaatattt acgatcaaac atgaatcttt ttctaatcga atctttgctg
     3781 caggcgcagt agaagcagcg gactggttga aagatcagtc accaggttat tacaaattag
     3841 aagatttatt aatggacaaa ggggtgtaaa aaacgtggaa gctcaagaaa tcatccgcta
     3901 cattcaaacg gcatctaaaa agacgcctgt gaaagtctat ctgaatgtaa ccgagccaat
     3961 cgaatttaaa gaaagtaaag tttttggtga aggaagcagc ttcgttgttt ttggtgacta
     4021 tgagacgatt gctccggttt tagaagctga atcagaaaaa atcattgaaa tcgaaatcga
     4081 aactgctgca agatacagcg cagttccttt gctagatatt aaaaaaatta atgctcgtat
     4141 cgaaccaggt gctattatca gagatcaagt gtcaatcggc aacaatgccg tgatcatgat
     4201 gggagcgatt atcaatattg gtgcagtaat cggtgaaaac acgatgattg atatgggtgc
     4261 tgtcttaggt ggacgagcga cagtgggcaa aaactgccat attggtgcag gagctgtctt
     4321 agcaggtgtg atcgaaccag catcggccaa accggttatc gtagaagacg gcgtactggt
     4381 aggtgcgaat gcagtgattg ttgaaggtgt ccatatcgga aaagatgcag tcgtggcagc
     4441 aggagcagtg gttttagaag atgtggcagc agaaactgtt gtcggtggaa tacctgcacg
     4501 tgtattaaaa ataagtgatg atcagacaaa agaaaataca gcattgattg ctgcgttacg
     4561 cgaattatag aggagatgga gaaaaatgcg cgaaatagat acctgtattg tgggggcaac
     4621 cggattagtc ggccaaacga tgctcaaagt gttagaagaa tatgattttc cagtaggacg
     4681 attgaaatta ttagcttcaa aacgttcagc cggtaaagtt tgtgtattca agaaaaagaa
     4741 acacactgta caggaattga cagaaacttc atttgaagga tatgatttag cgctattttc
     4801 tgcaggtgct tctgtatcca aagagtttgc gccaatcgca cgaagtcagg gactatttgt
     4861 catagataat tcctcagcat ggcgggaaga ttcggatatt ccattaatcg ttccagaggt
     4921 caatctggaa gactatacat tgaatcggct gattgcaaat ccaaattgtt caaccattca
     4981 agcggtactt ccgcttaaag ctttacaaga agcctttggt atccagcgag tcaattattc
     5041 gacctatcaa gcagtttctg gttccgggca aaaaggcatt gaagatcttc atcgtacgca
     5101 aaatgacgag gaaccagttt tgtatcctta taatattagc caaacggtga ttccggaaat
     5161 cgatcagcct ttggaaaatg ggtatacaaa agaagaacag aaaatgataa atgagacccg
     5221 taagattctg catcagcctg ctttgccagt atcagcaacc tgcgtacgtg tgccaatcga
     5281 aaatggacat ggagtctctg tggcagttca actgcaaaaa gagtttacga cggaagaaat
     5341 tagagagtgt ctcagccagt ttccagggat tcaattagaa gatgatctag cagcacataa
     5401 atatccgacg agcattcttg ctagaggaac agatatggtt tatgttggga ggatcagaaa
     5461 agacacttct ttagagaacg gattgttgtt ctatacgact gccgataaca tacgcaaagg
     5521 agccgctgcg aatgccgtac agattgctgc agctttaatg cagaaaatag cggaggtggc
     5581 aagatgagta atacgctgaa agtagcgaaa ttcggcggaa catcgatgtc tcatgaagag
     5641 caattcaaaa aagtccgtac aattgttctt tcagatccca atcgccaagt gatcgttgtt
     5701 tctgctttag gcaaacgtac gtcagatgat gacaaagtaa cagacttact ttatttgatt
     5761 tatgcttacc ttcagcatca tgttgattgg acaccccttt ggaaaaggat ttgtgatcgt
     5821 ttcattcaag taagagatgt tcttgccctg cagttaccta ttgaagatga actagcacgc
     5881 atacagaaac aactggaaga cgaaaagata actgaagatt atttagtaag tagaggtgaa
     5941 tatctgactg ctcaactgat ggctgagtat cttggttacc agtttattga cgcaaaagac
     6001 ctgattttct ttgactatga tgggaaaatc gatcaggaaa aaacagcaca ggaaatcaca
     6061 aaagcattta aaagatatcc gaaaatggtt gttccagggt tttacggggc gaaccctgca
     6121 ggaaaagaaa aattattggg ccgtggggga agcgacatta ccggcgcgat actggcgaag
     6181 atgttgcaag cggaaagtta tgagaattgg acggatgttt caggaatcat gatggctgat
     6241 ccgcgaatca tcgatcatcc taaaatcatc gaggaaatct cttttagaga actgagagag
     6301 atggcttata tgggggctaa cgttcttcat gaagcagctg ttttcccagt tcaagaggct
     6361 gatatcccca tccaaataaa aaataccaat gatcctttcg ctcaagggac aaaaatatcg
     6421 aatcacgaaa tagaaaagga aaatggactg acaggaatag ctggacgcaa ggattttctt
     6481 tccatcactt tattcaaacg tcatatgtcc gatgaaatcg ggtttatctg gaaagcaatg
     6541 agcatttttg ctaaacatga gatatcaatc gaacatattc cttcagggat cgacaatatc
     6601 ggagtgatcg tctcagcaga agcgattgcc gataagttat ttctcatcac gaaagaatta
     6661 aaagaaacac ttggcgtgga agaaatcgtc gtgatcgaag acttagcttt gatctctgtc
     6721 gttgggggtc cgcataaaga attgattgga ttatctggga aagtgttatc gatattgaac
     6781 aaattagaaa ttcgcacatc gatcttatca caaggagcac aggagttgaa tctgatcatc
     6841 ggtgtaccga ataatcaata tgaaacagta gtgaagggta tctatgaagg gatggtgaat
     6901 acggatgctg aaagaatatc gaatggcact ccatcaaatt cctgaaatcg ggtttcatga
     6961 attcaagaca aaaaaatatt tatatgaaca agtaaaaaac agcgaggccg ttatccatga
     7021 aatcggcgaa acaggactac tcctttattt tgataatcag caagaagaaa cgattgcttt
     7081 tcgaacagat atcgatgcac tgccagtcaa agaagaaacg aatctctctt ttgcttctaa
     7141 gcatgaaggt tatatgcatg catgtggtca tgacggacat atggcgatgc ttttagggtt
     7201 gactgacttt ataactaaac acattgagga tcttatttat aatgtagtac tgatctatca
     7261 gccatcagaa gaaatttccg gtggcgcaca aagcgttatt cagtcaggat tgttggataa
     7321 atatgaagta caagcaatat ttggctttca tctatggcct ggactagcta agggagaagt
     7381 tttctctcga ccaggacctt tgatggcaca aagcagtgaa acagacatta tcattaaagg
     7441 ccagtctgca catattgctt ctagtgaaaa aggaatcgat agtctggagg cagctgtacg
     7501 atttttgaag aatgtttatg attttgaccg gcaattaccg gcagagtgga atcatctaat
     7561 gaaatttggc caaatccaag gtggaacgat ccgaaatgtt ttagctggag aagttattgt
     7621 atccggaagt atccgttctt acagttcaga tatgcaaaat tatttgaaga ggggattaga
     7681 ccatttagca gaacagtttc actctacagc tactatttct tttcgctata atgacggcta
     7741 tccagctgta atcaaccata gaggcttata tgcagcactg ttagattcaa acaaactaca
     7801 tgaattagat gctccagttc ttcaagccga agattttggc atatatacgc aacaatatcc
     7861 atgtgtattt ttctttttag gtgtcggtga tacccctgct ttacatgatc cgacgttcga
     7921 ttttgatatg gatgttttag aaaatggtgt agaatggtac aaaacgattc tttttacaga
     7981 aaatcttctt gtaaaaatcg atagcagttc aaaagaggag cgagtaaata atgatgaatt
     8041 tatccaatcg atttagtcct aggctttcta gaatagaagt atcaaaaata cgacaattcg
     8101 atcagcaaat atcgtcgata ccagatgtga tcaaactcac cctgggagaa ccggattttc
     8161 caactcctga acatgtcaag caagcaggaa tagcagcgat tgaagaagac ttttctcact
     8221 ataccggaat gagaggatta gaggaactgc gggaagcggc ttgtatattt cagcaacagc
     8281 gttatggatt aacttatgat ccgcaaacgg aagttttgac gacagttggt gcaacggaag
     8341 cgattgcctc tgcgttgtta tctgttttgg aagaaggaga caaggttctt attccagcgc
     8401 ctgcttattc aggatatcag ccattagtgg aactagctgg tgctgaattg atcccgatag
     8461 atacttctga cacagggttt gtctgtcagc cggaacaatt tgaacgagca tttgaacaat
     8521 atggatcagc tgtcaaagca gtcattttaa actatcctaa caatcctact gggacgacat
     8581 tgtctgcaaa acaattgaaa gcaatcgctg aggttttaaa aaaatatccg gtatttgtta
     8641 taagtgatga agtatatgct gaattgactt atagtggaac gcatatgtcc atagctactt
     8701 atcttccaga acaaacaatc gtgataagcg ggttatccaa gtcacatgcg atgactggtt
     8761 ggcgagtggg ctttattttt gctcaaaaag cccttattga cgaactaatt aaagtccatc
     8821 aatatctggt gactgccgct acaacgatgt cgcaaaaagc tgcggctgaa gcgctaataa
     8881 atggggtgaa agatagcgag aatatgaaag agcaatatca aacacgaaga gactatctgg
     8941 tccagcaatt ggcgccttta ggatttgaag tgacccagcc aaacggtgca ttttacttgt
     9001 tttgcaaact cccagctgcc attcaaactg actcttggca gttttgtgta gaattagcgg
     9061 aacaggcaaa agttgcgtgt atcccaggaa ttgcatttgg cccagaagga gaaggttata
     9121 tccggatttc ttatgctagc agcatggaaa aattacatga ggcctgcaaa cggatcaaag
     9181 actttttaac agaacagtga ttcatagatt ttatcaaaaa aacaatcgtt aattttgcgg
     9241 tatattctgc aaggagacga ttgtttttta aatttagttc aaatgaacgc gtttcttttg
     9301 attcataact aatgtaatgt taagtttaaa ttgtttagaa tgattttaaa agaatattca
     9361 aggaggtttc atcaatggaa aaaatcaaag ttgtcattgt aggtgcatct catggagggc
     9421 atcaatcgat cttggaacta ttgacacgat acggtgaaaa tgtagatatc acgctatttg
     9481 aagcaggaga ctatatttcc ttcatgtcat gtggtatgga gctgtattta gaagacaagg
     9541 taacagatgt aaatgatgta cgtaatttca gaccagaaaa ctttccgcaa tccaatgttc
     9601 atattttaaa taatcacgaa gtaaaaacga tcgatgccga taaaaaaaca gtaacggtta
     9661 ctagcgcatc agataacgaa acaaaagaat atccttatga caaattgatt ttgagttctg
     9721 gtgtcaaacc aaacaatctg ccagtaccag gagcagatct agaaaatgtc tacttgatgc
     9781 gtggatacaa ttgggcaacc aaaatcaaag aaaaattaaa tgatccggct attaaaaaaa
     9841 tcgctgtcgt aggtgcgggt tacattggga tcgaagcagc agaagcaagc cgaaaagctg
     9901 ggaaagaagt cgtattgctt gatgtgatcg atagaccttt aggcacttat ttagatgctg
     9961 agatgacaga tattttagaa caacacctaa atgaaaacgg tattgaagtt ttgacaaatg
    10021 caaaaatcga agcttttact ggtaacggaa aagttgaggc aatcaaaaca agtgaaaaag
    10081 aaatcccagc agatctagtc attcaagctg ccggtgtgaa ggcaaataca gaatggttaa
    10141 aagggatcgt cgacttagat gaacgtggct ggatccaaac aaatgaatac ttgcaaacga
    10201 atcttccaga tgtgtatgca gtaggagatg ctacacttgc ttattctatc ccagcacgca
    10261 aaaaaatgcc gatcgcttta gcgactgttg cacgtagaga agcacgttat gtagtaaaac
    10321 atctttttga agaagtacca aacaaaccgt tcggcggagt cgtaggttcc tctgcattaa
    10381 gtgtctttga ctatcatttt gcagcaagcg gattgaatag ctttacagca gcaaaagcag
    10441 gagtagatgt acgagtgtct tattatgaag atacgttacg accaaaatat gtaccagaag
    10501 aacaaggaaa tccaaaagtg tccatccaat tattctcgga tccaatgacc catcaattgc
    10561 taggaggagc agtattatct acctatgatg ttacaggtca aggaaatgta ttggcactag
    10621 cgatccaaaa caaactgact ttagaagaac tagcagaagc tgacttcttc ttccagccag
    10681 gctttgaccg acaatggagc atgttgaatc tagccgctca acaagcgtta ggagaagaac
    10741 catttgtaga ataagaaaac gaaatatttg aaaagaggtt gtgaaaaagc tgccttgcat
    10801 caagtaataa gaacagccaa gtaaaaacag cttcttatgt ttttaacttg gctggactta
    10861 ttaccgcaga tgcaagcttt tgaacaccgt ttattttcaa aaaagggact gtgacacgat
    10921 ttgtgtaccg acccccaaaa gttagaccaa aaaatctaac ttttggaggt cggtattttt
    10981 tatggttaaa tatacttttg aacttaaatt aaagatcgtt catgattatt tggatggcaa
    11041 aggcgattgg accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaaattcgg
    11101 gagaagcaca aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc
    11161 tctatcaata aaaagaaagt tcagcgcttg atcaagaagc taggaataaa agtaacggca
    11221 tacacgcgta aatcacgtcg ctacaattct tatcgaggaa aagt
//
LOCUS       DASJTC010000089        11230 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.090, whole genome shotgun sequence.
ACCESSION   DASJTC010000089 DASJTC010000000
VERSION     DASJTC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.090"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            12..84
                     /locus_tag="U4B19_002227"
     tRNA            12..84
                     /locus_tag="U4B19_002227"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="U4B19_002228"
     tRNA            88..160
                     /locus_tag="U4B19_002228"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="U4B19_002229"
     tRNA            168..249
                     /locus_tag="U4B19_002229"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="U4B19_002230"
     tRNA            267..339
                     /locus_tag="U4B19_002230"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="U4B19_002231"
     tRNA            351..422
                     /locus_tag="U4B19_002231"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="U4B19_002232"
     tRNA            439..524
                     /locus_tag="U4B19_002232"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="U4B19_002233"
     tRNA            535..608
                     /locus_tag="U4B19_002233"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="U4B19_002234"
     tRNA            618..691
                     /locus_tag="U4B19_002234"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="U4B19_002235"
     tRNA            711..783
                     /locus_tag="U4B19_002235"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="U4B19_002236"
     tRNA            811..884
                     /locus_tag="U4B19_002236"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="U4B19_002237"
     tRNA            898..971
                     /locus_tag="U4B19_002237"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="U4B19_002238"
     tRNA            995..1084
                     /locus_tag="U4B19_002238"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="U4B19_002239"
     tRNA            1097..1170
                     /locus_tag="U4B19_002239"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="U4B19_002240"
     tRNA            1189..1264
                     /locus_tag="U4B19_002240"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="U4B19_002241"
     tRNA            1266..1336
                     /locus_tag="U4B19_002241"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="U4B19_002242"
     tRNA            1350..1423
                     /locus_tag="U4B19_002242"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="U4B19_002243"
     tRNA            1448..1535
                     /locus_tag="U4B19_002243"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="U4B19_002244"
     tRNA            1557..1628
                     /locus_tag="U4B19_002244"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="U4B19_002245"
     tRNA            1651..1740
                     /locus_tag="U4B19_002245"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="U4B19_002246"
     tRNA            1753..1826
                     /locus_tag="U4B19_002246"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="U4B19_002247"
     tRNA            1845..1917
                     /locus_tag="U4B19_002247"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="U4B19_002248"
     tRNA            1921..1993
                     /locus_tag="U4B19_002248"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="U4B19_002249"
     tRNA            1998..2068
                     /locus_tag="U4B19_002249"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="U4B19_002250"
     tRNA            2081..2154
                     /locus_tag="U4B19_002250"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="U4B19_002251"
     tRNA            2166..2237
                     /locus_tag="U4B19_002251"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="U4B19_002252"
     CDS             2503..3696
                     /locus_tag="U4B19_002252"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEN2077949.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="U4B19_002253"
     CDS             3826..5298
                     /locus_tag="U4B19_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2077950.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="U4B19_002254"
     CDS             5381..6169
                     /locus_tag="U4B19_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEN2077951.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="U4B19_002255"
     CDS             complement(6236..6904)
                     /locus_tag="U4B19_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEN2077952.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="U4B19_002256"
     CDS             7019..8731
                     /locus_tag="U4B19_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077953.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="U4B19_002257"
     CDS             8734..10506
                     /locus_tag="U4B19_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2077954.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..>11230)
                     /locus_tag="U4B19_002258"
     CDS             complement(10588..>11230)
                     /locus_tag="U4B19_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2077955.1"
                     /translation="LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLIT
                     KFLFLTGLRYGELISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAK
                     EIIDQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLR
                     HSHISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         3229 a         2011 c         2544 g         3446 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat
    10921 ttctttagca ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt
    10981 tttaattttt aactcgtaat cataagtacc agaaaccgta attttattac catcataatc
    11041 agattcagtc aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt
    11101 aattaaagaa taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac
    11161 ttcttctttt gataaataac catctttttc tttttttatt tcttcgtttg tcttaggttt
    11221 tcttgctaaa
//
LOCUS       DASJTC010000090        11175 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.091, whole genome shotgun sequence.
ACCESSION   DASJTC010000090 DASJTC010000000
VERSION     DASJTC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11175
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.091"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..305
                     /locus_tag="U4B19_002259"
     CDS             6..305
                     /locus_tag="U4B19_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2077956.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="U4B19_002260"
     CDS             378..1739
                     /locus_tag="U4B19_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEN2077957.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="U4B19_002261"
     CDS             1761..2021
                     /locus_tag="U4B19_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077958.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="U4B19_002262"
     CDS             2035..2787
                     /locus_tag="U4B19_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEN2077959.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="U4B19_002263"
     CDS             complement(2881..3486)
                     /locus_tag="U4B19_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2077960.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="U4B19_002264"
     CDS             complement(3539..4513)
                     /locus_tag="U4B19_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEN2077961.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="U4B19_002265"
     CDS             complement(4891..5610)
                     /locus_tag="U4B19_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2077962.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U4B19_002266"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U4B19_002266"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN2077963.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="U4B19_002267"
     CDS             7161..8558
                     /locus_tag="U4B19_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEN2077964.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="U4B19_002268"
     CDS             8639..9079
                     /locus_tag="U4B19_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2077965.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="U4B19_002269"
     CDS             9227..9553
                     /locus_tag="U4B19_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEN2077966.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="U4B19_002270"
     CDS             9554..10303
                     /locus_tag="U4B19_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077967.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="U4B19_002271"
     CDS             complement(10383..10838)
                     /locus_tag="U4B19_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN2077968.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
BASE COUNT         3550 a         1956 c         2294 g         3375 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt ataga
//
LOCUS       DASJTC010000091        10706 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.093, whole genome shotgun sequence.
ACCESSION   DASJTC010000091 DASJTC010000000
VERSION     DASJTC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.093"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(224..1072)
                     /locus_tag="U4B19_002272"
     CDS             complement(224..1072)
                     /locus_tag="U4B19_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2077969.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1071..2138
                     /gene="gnd"
                     /locus_tag="U4B19_002273"
     CDS             1071..2138
                     /gene="gnd"
                     /locus_tag="U4B19_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEN2077970.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2140..3141
                     /locus_tag="U4B19_002274"
     CDS             2140..3141
                     /locus_tag="U4B19_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEN2077971.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3138..3422
                     /locus_tag="U4B19_002275"
     CDS             3138..3422
                     /locus_tag="U4B19_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEN2077972.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3455..4252
                     /locus_tag="U4B19_002276"
     CDS             3455..4252
                     /locus_tag="U4B19_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEN2077973.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4266..5099
                     /locus_tag="U4B19_002277"
     CDS             4266..5099
                     /locus_tag="U4B19_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2077974.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5117..5974
                     /locus_tag="U4B19_002278"
     CDS             5117..5974
                     /locus_tag="U4B19_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2077975.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5996..6505
                     /locus_tag="U4B19_002279"
     CDS             5996..6505
                     /locus_tag="U4B19_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2077976.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6542..6943
                     /locus_tag="U4B19_002280"
     CDS             6542..6943
                     /locus_tag="U4B19_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077977.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7055..7408
                     /locus_tag="U4B19_002281"
     CDS             7055..7408
                     /locus_tag="U4B19_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077978.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7442..7885
                     /locus_tag="U4B19_002282"
     CDS             7442..7885
                     /locus_tag="U4B19_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2077979.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7902..8987
                     /gene="uxuA"
                     /locus_tag="U4B19_002283"
     CDS             7902..8987
                     /gene="uxuA"
                     /locus_tag="U4B19_002283"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN2077980.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8980..9693
                     /locus_tag="U4B19_002284"
     CDS             8980..9693
                     /locus_tag="U4B19_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2077981.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9900..10049)
                     /locus_tag="U4B19_002285"
     CDS             complement(9900..10049)
                     /locus_tag="U4B19_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077982.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10445..10645
                     /locus_tag="U4B19_002286"
     CDS             10445..10645
                     /locus_tag="U4B19_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEN2077983.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3574 a         1694 c         2318 g         3120 t
ORIGIN      
        1 gaaaagataa attttttact actgtatact aactttgtta tcgcatctaa caagtaactc
       61 gaataatacc aatacaacct tctccaaaga tgcgatgaca tacgttctaa cccaccgact
      121 attgatgttc tgtaccaccc ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg
      181 gctttttttg ttttatggat ttttcgcttt ttctgttatg gtgctattct ttgatttcag
      241 aaatagtatg tcgaacttta gcgatagaac gacgtgattc tttttcgttg taaaacatca
      301 aaatcacata tagagagtcc aaaatgctta gttgagaaat acgggaagct aaagcttcag
      361 atcggaattc tgtttcttca gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg
      421 aagaattagc ttggctagta ataacgattg tttttgctcc agtttttctt acggtttcag
      481 caatatggat tgtttctttt gattgccctg tgtgagaaat caaaacagca cagtcttttt
      541 ccgtcaataa agaagcttcc atcaattgaa tatggtaatc cgtgctgtga gaagttgcga
      601 taggggaacg caaaaattta tgataagcgt cagttgctac aatctctgaa ccgccaacac
      661 cgaaaaaata gacacgttta gattcattga taatagtagc cgcttttttt agatcctttt
      721 gttgtaacaa cttttttgtg tcatttaaag ttgaaatact agactcgaac accttttgcg
      781 ccatagtgag ctcagtatct gttttctgaa tattttcgtg gatagaaata gcagatagat
      841 cattttcttg gatcaacatg gccattttaa aatctttaaa tccattataa ccaagttttt
      901 ttgtaaattg aaaaaaagta gagtcagcaa tgcccagttc acttgctaaa ttgctaatag
      961 gactatgact tactttgctt ggattttcta aaatataatc ggcgatagcc tgttcttttg
     1021 ggctgaagtc gttgtacatt gttttgattt gtagtcttat ggagacgctc atgagaattc
     1081 ctcattttca taataagatt atcatttttt ccgttctcat taatgttaaa gatacaagga
     1141 gagtaggaaa aaatcaaaca aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt
     1201 tgtataatga gggtataaga aatggaggcg ctatcatgga aattggtgta attggtttgg
     1261 gaaaaatggg attgaattta gctttaaact taaaagagca ccatcacaaa gtacaaggac
     1321 tcgacatttc aagtacagct gttagtcagg caagagaaaa tgacattcct gcttatcaag
     1381 agatctctga atttctagct acttttgagc agaaaaaaat catctggtta atgcttcctg
     1441 ctggagagat aaccgaaaac atgttaagaa aactttttcc aaaactcgga ccaggagaca
     1501 ttattattga aggcggaaac tcttattata aagattcaat ccgtcgagca gaagagtttc
     1561 agaaaaatga aatcggttac ttggattgtg ggacttctgg aggaatcgaa ggagcacgac
     1621 atcaagcctg cttaatgatt ggaggagata aagaaacttt tctatccgtg gaacaattat
     1681 tcaaagatgt agcaattgaa agaggatatc tgtatgctgg tgcatccgga agcggacact
     1741 tcttgaaaat gatccataat ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat
     1801 tccaacttgt acaaaaaagt gagtatgctt ttgacttgga acaagtcgca cgtgtttgga
     1861 atcatggttc ggtgattcgc ggctggctga tggaaatcat ggaagatcag ttaaacgaac
     1921 atcccaattt aagtaattat cggggaatcg tttctgcatc aggtgaagca aaatggacgg
     1981 ttgaaacagc gctagaaatg gctgttccag ttccagtaat tgcaactagt ttgttcatga
     2041 ggaatctttc acaggaagaa gatagttttt cagcaaaagt agtttcagca ctaagaaatg
     2101 ggtttggcgg acatgaaata gttgaaaagg gtgagtaaaa tgccagatta tcatatagca
     2161 atcgttaatt ccagcagttt cggaagagtc tttccacacc acttggaaag attaaagtca
     2221 gttggagacg ttcatttctt tactgttgat caagagattc caggaaaaga actagcggag
     2281 ttacttaaag gattcaatat cattattgca agtgttacgc cattttttcg acgagatttt
     2341 tttgaaaaca aagatgaact gctattgatt tcccgacatg gtattggtta caacaatatt
     2401 gatttaaaag cagcaaaaga acaccagaca atcgtatcta tcatacctgc tttaattgaa
     2461 cgaaatgctg tagcagaaaa taatataact aatttgttgt ccatgcttcg acgcacgaca
     2521 caagcacaag caagagtcaa ggacgatcga tgggaagatc gggcggagtt cgtcggtagg
     2581 acgctgttta acaaaacagt tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc
     2641 gaaattttgc gtaacggttt tcgctgtgat gttctagctt atgatcctta caaatcagat
     2701 ttatacatcg aaagctttgg tgcaaaaaaa gtagaacttc aagaattact ggaacaatca
     2761 gatatcattt gtttatgtgc gaatttgaca gaagagaact atcacatgat ttctgataaa
     2821 gaagtcgatc agatgaaaga aaatgtttat ctatctaata gtgcaagagg cgctctagtt
     2881 gatgaagaag cgatgattaa tggtctgaaa agcggaaaga tcgcaggata tgccacagat
     2941 gttttagaag aagaaccggg acgtcaagat catccgtatt tagcgtttga acaagtagtg
     3001 attacgccac atatatcggc ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt
     3061 gtcagagatg tcgaacagat cagtcagcac cttcttcctg aacgttctgt ccagacaaag
     3121 agcaactata taaaaggatg atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc
     3181 ctcaatttta tttatatgaa gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat
     3241 acggcgcgaa aagagtactg gtcacccacg gaacggtatc atgggaaaag gcgctaccta
     3301 aattagtatt tttgaatgac gaaacgattc aattttttta tcatcgttat tctggagaat
     3361 gtagttatgc tgaagccaga cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt
     3421 agttggcgtt ggcggaggaa aactgaccga tctagtgggt tatagtgcca atcttgcaag
     3481 aatcgatttc ggagtcatcc ctactttagc tagtaattgt gctccatgga cacctcttgc
     3541 tgtcatgtat aaggagaatg gacaagcaga agggaaaaca gaacactttt acagacaagc
     3601 tgcttttttt ataacagatc cccaattagt gattgattcc cctgttccat atttcattgc
     3661 cgggttagca gatacactag caaaatggta tgaatctgag ctcatcttag aacaagattt
     3721 tttgcaagat gaatcatttt tgaagctagc acaagatact gcgaagatct gcaaagaaga
     3781 gattttgaat cattctgcta aagcaatcca agatatggca caaagaaaac tgacatcgga
     3841 gttcaaacag ctttcagaga ttatctttgc tgttgctggt ttagttggag gattaggaga
     3901 taaatacgca agaaatgcgg cagcacatgc tatgcatgac gctatcagta aatatattcc
     3961 ggaaagccat cgttttctac atggagaaaa agtagcgtat gggattttct atcaactggc
     4021 actagaaggt cgctgggaaa aaatagacga gttatatcct ttgtatcggg aattagaact
     4081 gccggtttct ctagaacaaa tgggattatt tccgaaagat aaacagataa tagataaaat
     4141 ggctgaattt attgattcga aagagaaagt gcatttgatt cctatagagg taacacagaa
     4201 atcactgata cagtcaatga tggaactgga aggttatatc agaaagatct gaaggaggag
     4261 gacacatgga aacattaagt gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg
     4321 ttatgtcccg ctggttaggt ggaggagcca cgttgacctt acgtttttct cctttgatga
     4381 ctggattagt cgtcgggttg atcatggggg aagttgcaca agcgatgatt gttacagccg
     4441 ctttgcaaat gatttatatg ggggtttttt ctccaggagg atcaatgcca gctgaaccat
     4501 ctattgcagc agctatcgca gtaccagtcg cattattagg ggatctgtcg ccagaagcag
     4561 cgattgccgt tgcggtgcca gtcggattgc tcggaagtta cttgtatcag tttcgttttt
     4621 tcatcaatac ttttttaggt aaatatacag acaaagctgt agaagatttg aatacaaaag
     4681 ctatggcccg atcaattatt ctttatccaa cggttgcctc atttttatta ttcgttccgc
     4741 ttgttttcat tgctttatat ttaggggcgc cattgatcgc tgatgtaatc aaagctttag
     4801 agggaacagt cgttatccac attcttgaag tagtaggtgg agggttagcg gcaatcggta
     4861 tcgcgacaac tgtatacgtt atcggtcgaa aagattatct ggtctttttc ttccttgctt
     4921 attttatgag tgttgttttg aaatctttag atattacgat ggttacttat gccatttttg
     4981 ggattattat cgctttgatc tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca
     5041 caagcaataa caattcagca tcttttgatg acgaagatga ctatgatgac ggattttaat
     5101 ttaggaggaa tgggaaatgg ctgaaataat ggataatcaa acaggtgctc ctgaagaaat
     5161 cacgaaaaaa gatgtaacaa aagcctatct gagatggcat tttgcaaacg aaattcctca
     5221 ctcttttgaa cgttacttgg cgccatcact actatacgga atgatgccaa tcttgaaaaa
     5281 actttataaa gatgaagact ctttaaaagc agcatataaa cggcagttac tgttcttcaa
     5341 cactcaatta agctggggtg gcggtgtgat tactggccta atgtcttcta tggaacagca
     5401 aagagcagaa gaagagtatg aagaaaaaga gatcttgatg caggatgact tgatgtataa
     5461 cacgaaagcg ggattgatgg gggcactagc aggaatcgga gatgcaatcg attcaggaac
     5521 cgtccaatat atttttatcg caatcgcggt accttgggca caacagggaa gtcctttagg
     5581 cgcacttttt ccttttatct gctttgggtt gtatcaggtt ttactagggg tatttttcgc
     5641 aagacaagct ttcactatgg gccgtaatgc tacaggccta atgcaaaaca ctggtgttca
     5701 gacagcgatt gaaatgctat cagttttagg gttgtttatg atgggggtgc ttgcgggaaa
     5761 ctatgtaaaa gtagaatctt cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca
     5821 ggaaattctt gatcaaatcg tgccagggat attaccttta gctgtcgtaa tgggtgtgta
     5881 cttgttctat acgaaaaaag gattgaaagt cacacaagca ttattatggc tgactggaat
     5941 attaattgtg ctagcaggaa tcggtatttt ataaaaagaa gggaacgtga ataagatggg
     6001 tgtagtcaat ttagcgcgtg tggatgagcg tttgatccat gggcaagtaa tggtaacgtt
     6061 atctcaaaaa agtggagtga attccatttt tgttgtagac gaagttgtag caaaagataa
     6121 atttatgaga gatctttaca aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat
     6181 tacaccagag aaagctaaat tctattggga tgaatatcaa tttaaagaat acaattgtat
     6241 tttgatcgca aaaacagtcc aaacgattta cgacttagta aaacacggaa tccccatgga
     6301 agaattgaat attggaggga ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa
     6361 atctgtttat ttaaataaat cagatgctga aaaattgaaa gaaatgaatg aacaatatgg
     6421 cgtaaagaat atttattttc aagccactcc atctgcacct aaaacaggat taaaagatgt
     6481 attgaacaag tttgatttgt cataaacaaa tgcttagaaa aaagataaag agggcgaaaa
     6541 aatgaagaag aacgagctgt ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa
     6601 gcgccgtaca aaaaagagta aacagcgttt tctttcagca ttagtatcag atatttacag
     6661 tatgagaaca gatgtgacag tcattgctta cgatacgcta gcctaccgat caaaaaatat
     6721 ttatgttgga gatatcgaaa aagcagagaa agttatctgt acttattacg atacacccgt
     6781 tcatgcatta ggaagttatt tcatgtttga ttggaaagac caaaggaaaa aaacaatcta
     6841 ctctatatta ttgtccttca ttttgttgtt cagtttaggt tggtggggaa tgatgatata
     6901 taacaaaaat cctcatcacg tattcgatct gctatccgta taaacaggta tcactgtact
     6961 agcatttggg agctactttt tcttattggg aaaagcagct cgcggatggc ctagcagaca
     7021 aacttttatt cgaaatactt catccatatt aacgatgcta gaaatgatcc gtacgattga
     7081 tgatccgagt gtagcgtatg cgtttgttga tgaaggctgt tatggagaaa agggcttaga
     7141 cagcgtgaga tcgggtatga aaaaagaggc tattcttttt tatctagact ctgttggggc
     7201 agatacaccg ctacagtttt cgggaaatta tttttcaaat aaagaacagt ggctgaaaca
     7261 agttgataag ttgaaagaaa aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc
     7321 gcagttcttc tatttgacca aaacagattt acgagggaaa acatttaatt ggcaaaatgc
     7381 caatcagatt attgctcttt ttcgataagt aaaaagtgca aaacaaggag tgagaagact
     7441 aatgttggaa atcatgatcg ttacacatgg taagttgagc gaaggactga aagatgcggc
     7501 ggaagtaatt gtcgggacat cgagccatat tgaaacaatg tgcttagagt ctggggagga
     7561 catccaaaca ttcggagaga aggtcaaaga aaaattaact atgatcaaca cagcagaacc
     7621 catgctagta tttgttgatc taattagcgc aagtccgtac aatcaagcgc tcttggcagt
     7681 caatgaactg actgaagaaa agaaagcagc cacttatgtc atagggggcg tgaatctgcc
     7741 aatgttgtta gaagcaatca atcatcagat gcttcacaca ccgatagaag aagcggtgga
     7801 acaaattgtc aaacaaggaa aagataattt aactgtttgg catgcttcag cggaagaaaa
     7861 aaaattagat gaggacgatt tttagaagga aggctgaaaa gatgaagtgg ggatttagat
     7921 ggtatggtga aaaaggggat tcgatcccat taaaatatgt tcgccaaata ccgggaataa
     7981 atgggataat tggaactttg ctgactaaaa tgcctggaga tgtttgggag atttcagaaa
     8041 ttgaggagtt gaaagcatct gttgagagcc aaggactgga actgcttgga attgaaagtg
     8101 tagcgatcca cgatgcaatc aaggcaggga cagatgaacg agattactat attgataact
     8161 atatacgaac aatcaaaaac ttgtctcgat gtggggttcg attgatttgt tatagcttca
     8221 aaccaatttt tggatgggcg aaaactgatt tgtttaatga aaacaacgat ggaagctatt
     8281 ctcttttata cgatcagaaa atcgtcgatc agatggagcc aggagaaatg tatcaattga
     8341 tacacagcca gtcaaaggga ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat
     8401 tcgctcagct ggaaaaactg tatgaaggaa tgaccgaaga agaattattt gaaaatctga
     8461 cttatttttt agaaaaagtg attccagtgt gcgaaaaagc agatgtaaaa atggctatcc
     8521 atccagatga tccgccatgg gaactttttg gttttcctag agtgacgaaa aatcttggcg
     8581 acttgaagaa aattctaaat gcagtagatt ctccatataa tggactaacg ctgtgcacag
     8641 gatctttagg ggcaaatccg gaaaacgatt tagttgaaat catccatgag gtaggttcgc
     8701 gcatccattt tgtccacttc agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa
     8761 cggcacatcc ctctacagaa ggctcccttg atatgtatca tattatgaaa gcgttggtgc
     8821 aagaagggtt cgacggtcct atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg
     8881 ctatgcctgg atatggcctg tatgatcgtg caatgggact cacttacatg caaggtctat
     8941 atgaggcgat aagtaaggaa aaaccactgg gagagcatta tgaataaaat caagctcttc
     9001 atctttgata tggatggcct tttattagaa actggacgtt tagcttatcg tgcttatgta
     9061 aaatcagcac aaaagtatga ttatgaaatg cgtaaggaag tctattattt actaactggc
     9121 caaacagaaa tggcgataag agaacaaatg ggcattttgt acggagaaga tgttccatat
     9181 atcaaatggc gagaagcaat caatcaatat aaagaaaaaa tcgtgaaaga agataagaga
     9241 gtatatacga aaaaaggagc agaagaaatt ttatcttttg caaaagaaag aggaatccat
     9301 acaatcgtgg cttcgtctaa tacacgggta aaagtagaaa tgtatttaaa aatggaaaat
     9361 ctatatgcct tgtttgatca aatcatttca ggggatgatg taaaaaaagg aaagccggaa
     9421 ccagagatct ttttgaaagc ttgttcaaaa atgaatattc ctccaagtga agcacttgta
     9481 tttgaagact cgattgcagg gatcgaagca gcaaggcgag cgggcatttt gtcttttctc
     9541 atcaaagatc atttgatagg gttgcccgat cataatggga agcataagtt gaaagtagat
     9601 gtaagtttat atgaatccaa taaagcagac tatgaatttt atgacctgca tcaagcaaaa
     9661 cgatttttag aaagtaaagg gttgtatctc taataaagag gactattttc tagtcctctt
     9721 tatttattgt cctgcacgac tgagggaaca gatgaaaaag aagtaatttt ttattttgaa
     9781 gtatctacct tatttgtagt gaacaattta gaacaaacac aaaacgcatc tactgttatc
     9841 taccagtaga tgcgtttaaa gagaagaacg agaatcgtcc atatgagggt ttccactcct
     9901 tatgcagatt tttttaattc ttgcactgct tgttcgatcg taacacctgt cgctacatga
     9961 ttggtatttt ctttgtcaaa aacgacaaat agattcaaat ctgtatccaa ggttactcga
    10021 tattgtatat ctttgttata gaatgtcata gccattctcc tttctttaaa tacttgctga
    10081 aaggcaacga acgcgttgat aacgaaattc catttataag tataccacca attgaaaggt
    10141 tttcatagtt aagaaatttt aaagtcaaaa gttgcagata aaagcaaata aacaagaaaa
    10201 caaaaagtga atcaatcgcg gttattcaac tttttcttcg attcgaagac aatagaagac
    10261 aatgaatttt atatttcatt gcattatcct tgtgttatta gaaaaagcat gatatattaa
    10321 atgagtaatc tattttgaaa cgtaatttga gcgatatatc tacatagata aaactccttt
    10381 tgcctatcaa atatttattg caacgaaaaa acgtacattg atacgtatca ggaggaattt
    10441 atttatgaat aacggtacag taaaatggtt caacgcagac aaaggttttg gctttatcac
    10501 tggtgaagat ggaaatgatg tatttgcaca tttttcagct atccaaggtg acggcttcaa
    10561 aactttagaa gaaggccaag cagtgtctta cgatgttgaa gatggtcaac gtggccctca
    10621 agcaacaaac attgttaaag cttaataaaa attgactttt aaccgtcttt tcgaaagaaa
    10681 agacggtttt tttgtacgaa aaaatt
//
LOCUS       DASJTC010000092        10068 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.094, whole genome shotgun sequence.
ACCESSION   DASJTC010000092 DASJTC010000000
VERSION     DASJTC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.094"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(26..178)
                     /locus_tag="U4B19_002287"
     CDS             complement(26..178)
                     /locus_tag="U4B19_002287"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN2077984.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(369..1247)
                     /locus_tag="U4B19_002288"
     CDS             complement(369..1247)
                     /locus_tag="U4B19_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2077985.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1286..2404)
                     /locus_tag="U4B19_002289"
     CDS             complement(1286..2404)
                     /locus_tag="U4B19_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEN2077986.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2401..4500)
                     /locus_tag="U4B19_002290"
     CDS             complement(2401..4500)
                     /locus_tag="U4B19_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEN2077987.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4551..5372)
                     /locus_tag="U4B19_002291"
     CDS             complement(4551..5372)
                     /locus_tag="U4B19_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2077988.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5369..6178)
                     /locus_tag="U4B19_002292"
     CDS             complement(5369..6178)
                     /locus_tag="U4B19_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2077989.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6199..6693)
                     /locus_tag="U4B19_002293"
     CDS             complement(6199..6693)
                     /locus_tag="U4B19_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2077990.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6705..7193)
                     /locus_tag="U4B19_002294"
     CDS             complement(6705..7193)
                     /locus_tag="U4B19_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEN2077991.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7270..9966)
                     /locus_tag="U4B19_002295"
     CDS             complement(7270..9966)
                     /locus_tag="U4B19_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEN2077992.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKSIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
BASE COUNT         3059 a         2265 c         1657 g         3087 t
ORIGIN      
        1 tattttatac ggctgaaaaa atctatcacc aaaaaaattc aaacattaag ccaaggatta
       61 tgaagtcaac atttcgaaaa atgaacatac attggtaagc aactataact attaccacag
      121 acataaagta acacaatata ttactattta agtgtacgcc accagtgaat atcctcaagg
      181 tatacagcgc acaacaaaaa gaaaaaagga aacgcagaag catttccttt cagtgattta
      241 atgaaaacaa cacgatttga cttagagcct ttttatttga attcattttc gctttatcca
      301 acttcatttg atcatttgat tactcggtta aatacttttg acatgagtat caatgaaaca
      361 ttcattaatt atggttgctt atttcttacc gcttttctgg ccaatgctaa tccgccgatt
      421 attccggcat tatcacctaa ttcgggcgta ataatgtagc tatcaattgg tggtatttca
      481 acatatccat ttataagctc aacaaattta tcctgaactt ttttcttcat gtgttcttgt
      541 ttcattacac caccgccaaa aataattcta tctggcgaaa acatcagtgt tgtattgtat
      601 gcacattggg caaggtaaaa ggcttcaatc tgccaaaacg aatcgtcagc caataaattc
      661 tggcctttta cacccaaccg tttttcaatc gctggtcctg ctgccatccc ctctaaacaa
      721 tcttggtgga agggacagtt tcctgaaaaa ttgtcctttg gatgtcgctt gatcaacata
      781 tgccccattt ccggatgact gaacccttcc acgaaagtcc cattgaacaa ggcaccacct
      841 ccgatacctg ttcccacagt gaagtatgca acacctgcta accctttgcc agaaccagca
      901 acatattcac catagcaagc cgcattgaca tctgttgtcc attcaatcgg aatattgaaa
      961 tattgcttta gcgttcctac aaaattgaaa ttttgccaag caagttttgg tgttgatgtg
     1021 atatgaccga aactcgttga tttgcattga atatcaattg gaccaaagca gccgaccccg
     1081 atgcctgcca attgttcctt aaaaggttta aaaaaatcaa tgacctgctt cattgtttca
     1141 actggagttg ttgtgggaaa actgactctt tccaaaatcg ttaattcctc agtaccgact
     1201 gcacagacaa atttagtccc tccagcttca attgccccat atatcatcat aggttcctcc
     1261 cttcgaatat gtgaaaaaca cttcattaac gcggcaagcg ttctcgatca gtgaaatgac
     1321 gtttagtatt tcctgaagct tctgttgaat accagtaagc gacactagca acatcgtctt
     1381 gccgctcaaa taagcgaaca tcatcgttgc cgatttgttg taaagtgacc gataaatctt
     1441 cggtaaaaca gatggggtct tgaagatgcc aacgataaag accatgtcgt ggcattgcgt
     1501 cgtcaccaaa ggcatgaacg gcattcaatt ttcccgtttc aaaacgatct cttgtggcat
     1561 ctctatttga ttggtaagga taccctaaaa aggctgttga atagtttttc gcatgtggac
     1621 gtccgaactc atcgtactca tgaaaggccc acgccccgcc aaaataatct tctgaacctg
     1681 ttgaggttac ggtcggatac tcactatccc catcaatgta gaatttaaac tcgccttcgc
     1741 cccaccaata acgttctagg caggtaagcg caaggtaagt acccacgtaa tagccttgac
     1801 cctttaatcg atcaattaac ttataatcct cctgtaattt ggtaatgcgt tcccgatgcc
     1861 aatttgcgtg aaaatatagg ctattttctt ccaattcgtc caccaaagcg taatttatcg
     1921 tataaaaaaa tgaggacata tccttcggat gtaaattctc gatcgtaatt ttagcggatg
     1981 tttggaaagg catttcgaaa taggaattaa agccccctgt aggattaacc actatcggca
     2041 acgagttcac ctcgcaacgc tctccaaacc cattacaaaa gaaatcccct aaaggacttt
     2101 caacagcagg gctatccgac ccatcccaat aaattcttaa gattaaatca cgtaaaacaa
     2161 aactgccaaa ttctgttcga tcacgaatcg tcatccacat atgacgaatt attcccgttc
     2221 cagtaatatc cgcaatcaca gtttcttcac cagaagtcag cggaattgcg ccacgacctt
     2281 tgcggcttgg tcctagctcg cttggcaaca tcgcagcttt acctttctcg cctgttggat
     2341 tttcgggagt gattgctcga ctctggattt ttttaaattg ggtaatattt tttagtatac
     2401 tcatcttttt ctcctttgaa tgtgaatcgt ttttcccttt tctacgtgaa gaattttttt
     2461 cgtatcactt attttcaatg gcttttctga caaatttgaa atactcaact cggattgcgt
     2521 acattcaatc gaaatgaagg cttgaccgaa cttcaaattc ttccaagaaa acgttaaatt
     2581 atcaacagga tcaatttgca atactccttt tttgaatttc aatccgaaat attgactgat
     2641 cattaggctg acaaaaagac ccgatgccca gccgcatttg ccgcagccaa agtctcgcac
     2701 gacttctcct tgttgagcct ctaagcgata cggccaccac caccacgaac cgtctaaatc
     2761 tgccaaacaa attagttctt ctaatcgggt tacgaaagct tgagggttgt gcagctcgct
     2821 cattaaaaca gtgataaagc caggaaaagt ggcgccggat tctaatcccc aagtgatgcc
     2881 tttgatttcc tcgctatagg ttggattgaa agaactagca gaaaaggcca tggtgttctg
     2941 atataaatga tctttattat ctaaaaattg ataaaaaggc atcaacgtag tgtctgattc
     3001 ttctccatca tgcatcaata aattaatttt tccatccaca atcacatcac ttaaaaagat
     3061 taacccttgc tcaagaatcg gtttttgata gtgcttcact gaataggttg ttttctcctc
     3121 atcaccgatt ccctccaaaa attgctcacc aaaaggacca tcgatagtca tttgtttaca
     3181 aattgattcc tttagtttac aggcaatgtt tttgtatcgc ttgctaagac tcagttgttc
     3241 tagggcttca aaaatttctg caagtccaat acaggccttc caaagacaaa gattgctgcc
     3301 ggtatgatac tttccaagtg caaaggcgtc tgataaccaa acagatggaa aaagcattgg
     3361 ttcccctgac cgacgttgag ctaatatgtc ctcaataatt cttgaagcat tcagtagaac
     3421 ctgcggacac cgctcaaaga attttgtatc cccagtatgt tcaaaataaa gactagccag
     3481 taacgcactg gataacgaat tactcagaga atgattgata cctcctgtaa attttgaacc
     3541 aggaaactta acgccatatt gatcaaacca tagaaccgtt ttttgacata actccggttc
     3601 aaacatggtg aatggcaatg atgcgtaata catatcctta ttccaaattc gattagtagc
     3661 aggatagctg ccccaattgc tgccgacgac ctgtccctgt cgattcatac cgaaactaga
     3721 aaagctttga taaagtgcac gacgatagag atggacaact ctatcgtttg gcattgacag
     3781 ctgcccaaat agctgagcaa aatagttcaa tgtttccttc agccattttt cgatatcaga
     3841 ttcctgaaaa gtcattactt cttcaaaagc atttgggtcc acaaaagcaa tggcgaagct
     3901 tgccacttcc tgttgtgcga tcgtgtcctt atttcctttt tgagaaatca acacattttg
     3961 ctgatccgaa tacttctctt gatacgaagg cacatctttc actgaaactt gaaggctttc
     4021 gtgtgtttca ttcacaatgt aaacctgttg aacaagcata cttaaacgct tattagccag
     4081 tatgggagca aaggatacta caatgacctt aaacgtcgaa taatgatgaa tatatctagg
     4141 caaacagtct tgaatcacat cgacttcaat acgatgatcc gattctgaca aatggtagac
     4201 aatcccgttt acttcaacct ttaaggataa cgaattggtt tgagagagct gtttattgac
     4261 ccaaacacct ggtttttcct cagttagtaa attttctcga taaaaggtca acgtttgaat
     4321 cgtcccgcaa acatcaaggt ttgctaatgt cttttgatta cttacatcga aggtcaatcc
     4381 ctttacatca ggaatattcc gatagtagct gtattcgttt gaagtagtca catttccgtc
     4441 gtttaactca gcattaaaaa atgggtgttt gggtgaataa attacttcat taatagtcat
     4501 cttttccctc cttgctaaaa aaactgcagc ctagtagctg cagcttccta ctatgcgatt
     4561 ccaagtaatg acaataaaat tcccaagacg accattccaa aaataatttt gatgacgccg
     4621 acttttttat ctagtaattt gaaacagctc aatgttacca ataacggtac aattccttta
     4681 aaaatactat cgaggatttc ttggacattc attagttctt cgcccttcat tgaaaagctc
     4741 aaaacggtat tgaatttgac catactgatc gtcattgcac ctaccatgat cagacccaag
     4801 atactagcag cttttgtcag aatttcaatt aacccgttcg catacagctt ttcaatatat
     4861 ttagagccta atgaatagcc taaaacgccg ccatagtatc gcatcaagta attaggtata
     4921 ttgaacataa ctaaaaagat aattggtgcc aaaatatttc cggcattggc taagttgatc
     4981 gctagtcccg cggtaacgac acgccaaact ccccagaaca atgaatcgcc aattccagcc
     5041 aatggtccca tcaaactcgt tttgatcgcg ttgatacttt ccacgtcaaa accagctttt
     5101 tcagaatttt ccttctccat cgatgccgca atccccatga caaaggacgc gcaaggaatg
     5161 gtcgtcgaaa aatactgtgt atgacgttcc agcgccgcag ctcgatcttc ttcacttttg
     5221 tagaaatgat taatgaaggg catcatagaa taggcgaaac caccgccgcc catcgtcacc
     5281 ggattcaccg aagcatacat agtcatcgag cgccaaaaca tcttccgaac caattttttc
     5341 tcatctttag aaattccatc agctaatttc attgaaagaa atcctcctct tcttggtcaa
     5401 gcgttgcatt tacagcagca tcgctttgaa ctgcatgaac agatatattt tttagttgta
     5461 gatctctttg ggctataaca attgcaataa tcgttccaac tgcggcaatt gcaatcaatg
     5521 gtaactcgag atacgccata acaacggccc caaagaaaaa ccacacagca atatttttac
     5581 tccaaagcat ccgtaacagc atcgccatcc caactgccgg cagcagattt ccagcgactg
     5641 ttaatccgtt catgatgacc tctggaatat tatccaagac gcttttgaca acatctgctc
     5701 caaataaaac ggcaaagaat ggaataagag cataaagcgc atagtggaca aaccacataa
     5761 agaagtgcag attgatgatt cctttttgat caccagattt tgcaaatttg aaaaatttag
     5821 cagccatcgg tccacataca aaagaaaaaa tcatattcat taactgtaat cctaatactg
     5881 ctagtggaaa gacaatggga ataatcgcat ccacgtttcc tcccattttg attgcgaacg
     5941 ctgtagccag cccagcagcc aaagaaggct ccgccgtact agagattccg atattgacga
     6001 cacccataaa gatggcttct agtgacgccc cgacaatcaa ccccgtctgc aaatccccca
     6061 tcaacgctcc tgctaacgga gcaacaacaa tcggacgact cagcatggac atcccgaaca
     6121 tttcatgtcc agcaactcca ataaaaacgg ccaaagcgac tacaaatgca tcatacataa
     6181 tttttctcct tcctttttct aaaaatcact caaaatagag atagctgaac gtttttccat
     6241 cgccggtgta ggctgaaaaa cggtatctgg ataaagttca actaattctt ttaagtcttg
     6301 aatttctgac gtcgttaaca tcacttcctt tgataaggtt ttcttatctt ccgatggcgg
     6361 tgtcattttc ccagcattcc ccacgttcat taattcgatt tccgttggga aagctttcgc
     6421 aacttttacc gcatctttga cattagggac taaaaccatc aagcgcatgt ccttcgcccg
     6481 cggatctttt aacaaatgaa tcgcgtcatc aactgtttta ataatgactt tgacattcga
     6541 tggtgcagcc atcttcaatg tcatcttttg aatatcgttg gtagccgcct catcgcttgc
     6601 tactactaac ccggttaaat ttagactctt tgtccaagtc atcgctactt gtccatgaat
     6661 taatcgttca tcaattcgca ctgctttaat catttttctt cctcctaaaa aaaatcttcc
     6721 tcttcattta ctacatgtac ctcatcaaga atggtcatct ttaattcctg ttggcattta
     6781 tttaccagtt cctgaatatc ctcctttgtt aattccttgg ttgccaatac tatttcgagc
     6841 agaattggca gattaaagcc agacaaaata gttacagcaa tctctttctc caaaaaggtt
     6901 ttggtaatct tctgattgac acttccgcca aataaatcgg taaaaacaaa aatctgatcc
     6961 tgctcgttca cctggtttac aaatacattc aactcctgtt caaaatcaca ctcatccaca
     7021 taagcattaa tcaccgtaat ctcttgaacc ttccccgtca gtaattgaat actgctaaga
     7081 aagcctgaag ctaagtgacc gtgagtcgct attagaattt tcttcatatg gttaattcct
     7141 ttctttctgt tcacttttat ataagcaatt cctgtgccaa cttttaaaaa cattggttta
     7201 atcacgttaa ttcacattaa aaaaagtgtc taacctaaaa aaaacagatt agacactttt
     7261 ttagacactt tatttttgaa tatacggtcc taataattca tagagcagcg aaagctcata
     7321 agcattgact ttaaaattat atttcatctc cattgattga aaaatacttc tagaaacata
     7381 ataaaattca tcttcatctg gtttcaaatc ataattcggt tcttcagatt tttctgtccg
     7441 agccaagaat aaacgttcca tcatcaatgc cgtatgcata tagagattta gttttacacg
     7501 gccatttaaa gtgaaatgat agtaattttc atatttagaa atgactgttt ccacttcact
     7561 catcacgatc gttgggttca gaaagttcaa acggtccgca attccttcca acgaaaagaa
     7621 ttttactagc tcctgaatca agcggttaaa ttctgccggc ttcatttcag gactcaaaat
     7681 atgctccaaa ttggctgctc catcaccgtc taagatgtca taaacattaa tattaggaat
     7741 tgaaaaggaa cgtggaagat ccgaagtcgt gatgaccaat tttgtcttgt caaaataagt
     7801 ttcatcattt tggtcgatta attcacgtag ttctctgtag tccttcgtga aaacttcgat
     7861 ggattcatcc gataaatgat gacgaaaaac atctttcaat ttgtccgaaa tccccaaacc
     7921 cgacatacag gaaatgataa tgttgctact ttgagaaaag ccttcaaagt attgaacatt
     7981 gattgaatac ttgtctgctg catcctccgc aatcgattta aatggcgcat tcatcgtcat
     8041 cttcaagcca atatcaagtg caatggaagt cgtcaaatta ttcagaagaa gtaactcccc
     8101 ttttaaatcg ttcttgatct gtgtgtaaat ttgatttaaa gagcccatat ctacgatcag
     8161 cactagacta tctgtattca cttgccgttt cacgaaattc cttgtcgatt cgacaatctc
     8221 tgacacatta ctctctactg gcatatccaa cgcttcaaaa acataattgc cgcataattg
     8281 attcactact gcttgaatgc tgctagccgt actatctccg tgagcaagca acaggccctt
     8341 cagctcgatg gattcatcta cataatcaga taaagtcacg acaataagca gtcttagaaa
     8401 gttctcgtcc ttttctgaga gcggtggtaa atgctccaag aactgattac ataagtaagt
     8461 cgtccgaggt agatttgcag ctaatgtacg atttatcccg gttaagtctg tcttatagct
     8521 tggggaaatg acataggatc tgctaataat ttctgcaaca ggtccactcg tcgtcaatcc
     8581 gtacgttttt cctaaaacgg atacccagac ctttctaaac atctgaatca tcaattttct
     8641 actcattgat aaaaaagaat ccttatcaat gtgatgcacc aaatgctgta cttcattctt
     8701 tatcgaagaa aaattttcat tgaaattacc ttcatttatc agctgagtaa ttttttccaa
     8761 aatcgaatca taatcaaacg gtttgattcg atccgttttt atcttttgat ttttctttat
     8821 caataaacta gcaatcggca agctttctac ttcggattcc agcatattta actgaatcaa
     8881 gatctcattt ttcccaatcg ttaatggttc ttcttgtgtt cgactataca catccgcaca
     8941 actgatttta atgatattct tcagcttacc aatattccca ctaaagggat gagataacat
     9001 taaagacaag gcttctcttg aaacaataat atctttttgt aaaacatccg cttcattttg
     9061 aaaaaataat tgaatcagct ctaatcgttc tcgaatgggg cgatcttcaa aagttggcaa
     9121 cataacacta atttgaatac gccgatcaaa ggtgtctaat aaataatttt cacctttttc
     9181 cgtcgtggcg aaaataaaac gaaccttagc agaatgccaa tttacatttt caccgactgg
     9241 acgatagttt ccagtatcaa taaatagaaa gagcttttct tgattttctt tcgataggcg
     9301 atggacctca tcgagaaaaa gatagccgtt gttggccgtt gccaataaac catcggtatc
     9361 cttttcagcc cccgtaaagg cccccttctt gtatccaaac agtgttgccg aaagcagttc
     9421 gggattgtcc gcataatcgg cacagtttaa gatagcaaat ggtgcatctt tttcaataat
     9481 ttgctcatgg atagcgtatt caaaaatttt tgtggctaaa aaactttttc caacacccgt
     9541 ggctccattt attaatacac tgagtccgtt aggcggatat ttgacagcgg ctatacattt
     9601 ttgaatgacc tctctcagac ttccgtcatg accaactaca gaatgaaagg cttcgtttat
     9661 ttgctccgta ttatctacaa ccgcatattc atctaaaggc ttccacaaaa caggctttcc
     9721 cggtaatttc tcgacctttt tatcttccac taatctcgtc aaataatggc tggcattctg
     9781 acgagagata cctagattct tcgaaatttt ctcggcagtg acagaagaca tattccctgt
     9841 tagtcgtatc agctcatcaa aaactcgatc tttagcgctc tttttcattt tttcctccag
     9901 tttaatgtgt ctaaaagcat aataaactat cggctgcaat atgtctattc tatatttgta
     9961 ttccaatttt tactttgaaa cacaaatagt cgaatatttg cctcagatat ctgacaaaat
    10021 aatttatcta ccaatttagt ttttatctaa tatgattttt atgttttt
//
LOCUS       DASJTC010000093         9467 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.095, whole genome shotgun sequence.
ACCESSION   DASJTC010000093 DASJTC010000000
VERSION     DASJTC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.095"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..330)
                     /locus_tag="U4B19_002296"
     CDS             complement(<1..330)
                     /locus_tag="U4B19_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077993.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKC"
     gene            complement(337..885)
                     /locus_tag="U4B19_002297"
     CDS             complement(337..885)
                     /locus_tag="U4B19_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEN2077994.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(930..1262)
                     /locus_tag="U4B19_002298"
     CDS             complement(930..1262)
                     /locus_tag="U4B19_002298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077995.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(1276..1533)
                     /locus_tag="U4B19_002299"
     CDS             complement(1276..1533)
                     /locus_tag="U4B19_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077996.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(1728..2270)
                     /locus_tag="U4B19_002300"
     CDS             complement(1728..2270)
                     /locus_tag="U4B19_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077997.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            complement(2291..3382)
                     /locus_tag="U4B19_002301"
     CDS             complement(2291..3382)
                     /locus_tag="U4B19_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN2077998.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(3390..5600)
                     /locus_tag="U4B19_002302"
     CDS             complement(3390..5600)
                     /locus_tag="U4B19_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2077999.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(5616..8144)
                     /locus_tag="U4B19_002303"
     CDS             complement(5616..8144)
                     /locus_tag="U4B19_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEN2078000.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            complement(8196..8606)
                     /locus_tag="U4B19_002304"
     CDS             complement(8196..8606)
                     /locus_tag="U4B19_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078001.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            complement(8619..8837)
                     /locus_tag="U4B19_002305"
     CDS             complement(8619..8837)
                     /locus_tag="U4B19_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078002.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            complement(8834..>9467)
                     /locus_tag="U4B19_002306"
     CDS             complement(8834..>9467)
                     /locus_tag="U4B19_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEN2078003.1"
                     /translation="FIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIRSMNP
                     ESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYETVDA
                     SEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKETKKPF
                     IVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
BASE COUNT         2899 a         2045 c         1350 g         3173 t
ORIGIN      
        1 acatttaatt tttaaacaaa attctcccca aaaaggaact ttttctagtg aaaacttggt
       61 aatctctttg taattaattc ttgttaaaga ataattaaac acttcatttt tacttgttat
      121 tttttcattg actataacta gttcatctgg taaaaaaatt aaccaacaaa catcgacaaa
      181 cagtaaatga tataatccat ttccacctcc aagcatattc aatactggtg aacatgcctt
      241 agaaacacga atatagttat ggtttaaata gatattttct ttaaacattg cttgtttaaa
      301 agccatatgc tcttgttcta aacttttcat aaacttttac tctaatgcac agctagcagc
      361 agctgctgca attcctgcta caatatttac cttagctaac tctttagctg cagcttttcc
      421 taattgtttt tctgcaattt ttaataataa atccgcagct ttttcaaaat taaaagcttt
      481 taatgcttca ataaaatctc cctgcgtacc tatagcccaa gccactcctc ctccaggaag
      541 agaattaatg gctacacatc cagcatatcg tttccaatca actttctcca ttctaggaac
      601 aataatatgt tcataaaaga ctcttgctgc tcttccttgt tcgttatctt gactgattcg
      661 ttcacgaata gcaccaaaat cctttatatg gtatacacca tgttcgtcta aaaaaccaac
      721 tgtatcaaaa taaaaagtta attcatctac taatttattc tttatttcta aatcaactgt
      781 attcactttt tctgtggcat acacacttgt tccagtacta acaaaagtac ttaaacctaa
      841 taagaaaaca cctaaaacac caaatatttt aattattctt ttcataattt ctcctccata
      901 aaaaataagt tactttttaa atatcttgtt tatgaattca ttccccaaag gccttttaag
      961 attgatacag cagtccgttc acttctttta gacttgtcat acaagtcttt taaatgatcc
     1021 catatcagac tagtttgtct atctagcttc aatccattaa acgctaatga acggttcatt
     1081 ttttctatta ctttagataa agctacagaa atttttatac cttcacaacg cgcatcttgg
     1141 atttcaataa tagtatcatc aattagtttt tgaatttctt gcctatcaac atattcaatc
     1201 ttagaaagag aatcttttaa agctactaat gattcaacga tttctttttt ctcttcagac
     1261 attttcttcc tccttctagt gacccgtaaa gccatgtaga gcagagtcca ttgaaccagt
     1321 aattacacta tctacaacgc cattccaatt tactctacat tttccatttt tcagatttgt
     1381 cggtccatac ggatagcctt tttgacattc aatgttcgga ttataccaga taggattata
     1441 ccattcagcc gcagaagcag attcagtcga tacaaacaat cccacaccaa caaatccaat
     1501 acacatcaca ctatagccaa ttagtttttt catctcgtat ccatctccta aaaaatagaa
     1561 tagttcttat agttattcat aagacatatt ttttccgttt ttgttttatc ctttctgtcc
     1621 tataacaact tttttcaagt aaagaatgac atagtttttc ttaaaaaaac agtctgtttt
     1681 tttctatttc ttcggtgtat aataaatatg agtgttcatt ttttatttta atacgcactc
     1741 cgtacataat aaatcttgat cgcatcaatc ttttgagctt cttgacctac tttcaattga
     1801 agtaatacaa attgagtaac cttattcatt gtttcttctc caaataacaa atattgatcc
     1861 ggattctcta tatcttgtgt cacctggtaa agtttcccag ttgttttcat ttgtacatgc
     1921 gggtctacat caatactatt gtctttaata caacgatctg tcatatagtc cctcacagct
     1981 tgattgcgtt tataaacatc tgtgaaatta ctccattttc tagcaaaatc ttttaagaaa
     2041 ttttttgtca tttcatctat ttgcatttgt tgcgctgtct cgtcttctaa tgaaccaatt
     2101 tgactgttac tttcagtact ttgaagagtt ccacttgttt cttttactgc ttcgattgtt
     2161 tcttctgtcg ttctcaattg tgttttttgt ttttcagaag aaaaccaatg acaaccagta
     2221 agacctatca aacataacca actaaatact aaaattatcc cttttttcac gtttctagtc
     2281 actgcctttc ttatttcacc ttacgaacca gactacgaat ttttgtttga ttgtacgtgc
     2341 gttgataccg agccataatc cgtccttgct cggcattctg ttccacacta gagaaatgtt
     2401 gtccgccata tgaaacatct gttataattc cggtgtgacc atagattcca ggagacaatg
     2461 caccacctgc ttgccaattc accacatctc ctgcttgtaa atcacttggt tttgggttca
     2521 tgattacttg ccaaccatag gttgtccagt tataatcttc ccctattaac atcgcaaaca
     2581 tgtgtccaga acccattaac tgtgggccac ctaattgaga tacataccat gccgtaccac
     2641 cataacattc cccattattt acggatcgtc caatgatccg atccaaaacc ttcgcattgc
     2701 ctttactttt tcctgcaata agcgaagttt tttttgttgg taggctattc caatgcttga
     2761 tcgcaaaagt atcatattcc gttaagttat aggacgttat gatactcatt gctcctcgat
     2821 aatacggact cccttcggta atacctgctg ccgcatagcc tccacgacct agaatcaata
     2881 aacaacttcc aggatcttta ttgcccacac attgattaaa gttgctttgg ctcgccacca
     2941 taaagccata atagtcataa caagctttta agttcggaaa cttcatatag aaaccacctt
     3001 cagaaccacc aattccacgt gcagaacctt gtgggtaggg acaaccagga aaccaggtaa
     3061 tcccaaaaaa gttcgtatcg tttcttccag attgtgactg tccataagct gattcatagc
     3121 ctaattgaga aagaatcaca ctcggtaata actgatactt tacagctgca ccaactgctg
     3181 cttgcgcatg ttcatctgtg attggaccac gtcctttaat ctctgaaaaa atcggtaatc
     3241 cttcggtata ttccccatta aaagcgacag ctagacctgt agcttctcct ccatgctcag
     3301 aaagttctcc tgaaaaaaag aatggcaata gaaaaaaggg aagaataaag aaaagaagac
     3361 aacctagaca ccctttcttc atttcttcct catcctttct tcgataatgg tttttggact
     3421 gttcgtcctt ttggtaaagt aaaatcagtt cctggttttg cgtccaaaat cggcagtgtt
     3481 ttaggatcct tttctatttt ttccgattgc tctgttggct tatctaaaga agaaaatgca
     3541 ggttgtaacg attttggtgc ggaaatcgac ggtttacttt cagacgaacg ttgccccggt
     3601 tgttgcacag aacgatttcc tgatagagtg gctaccgacg aattaggaac agagtattgg
     3661 ttcaataact gctgcattgc tggataactc ccatttttcg cctgattttt cacttggcga
     3721 atctgttgtg aaagagaacc agcagctgtt aattgtggca cgggtacttt tccagaaggc
     3781 aacggaatcg attgaccaac aaaagcgata cctttattta acaatggttt tctttgatta
     3841 ttcccttgtt gcttactcaa ttgtgccaat gattgtacct gttgttttgg tcttactgaa
     3901 ttcatggacg aatttggaat caaaccattt gaaagaccag aattccactc tgttttcgat
     3961 ttggtagaag acatttcagg ctctacccca tgataacgaa catgacgacc actataaggc
     4021 aaggcttgat ttgaacgccc acctagttca aagggattaa ccacttgcct cccttttacc
     4081 gattcttgtg gtgtacgttt ttgttgaaac ttttgaaatc catgtatcgc tgcagaagcg
     4141 agtggattcg ttgctcgata cacattccct gctaatcctg cacctttgat tgccaaattt
     4201 tgtccagcag ttgatagttg accaggcagt tgtttcattt gagacaaaga atgacttgct
     4261 atgtttttcg tgttgtttat agtttgatct gcagctccta accctagttt ctttaataaa
     4321 tctttccgtt ttacaatgag taaaattgat aggagtgctt tgacaaacat atttcctaaa
     4381 aaaccaaaaa cagtattcat tggaattaaa tcgtctacta tgttatagat caaaaatgcc
     4441 accaaaataa tcacactgta aaaagatttt tgaaacatca agcccaaaaa ggactttaag
     4501 gtattcatga gcatataatc aaatactgga acaaaagaag cgagtaatga aaaaggaatc
     4561 acaagtaata aacacaacac caacatttga aagataaaac gaatcatgcc aatcactaca
     4621 agtggtaatc caatacttag attaagcaat ggcaccatag aaccaacgac tgctttataa
     4681 acagctttag ttggttgaat atagacacga taagccttct ttttccctct atcttctcct
     4741 gcagcagtgg tcacttttgt ctcaatatca gataattctt cacttgacgc ttccttggct
     4801 agaaattctt gtggttcaat ctctgcttga tacaattctt ctaaatttgt tgtcccataa
     4861 tttaacaata tatacggatc aacaacagcc gctttataat aaagttggcg cactttttga
     4921 atggcttcct tttgtgtcgt tggcactaaa acaccattga catcttttgt cgccaattca
     4981 tctagactat ttgtagcaga aaaaataaag ccttctacct ccacggacaa atcatttaac
     5041 cctttggtaa aagcttctcc tttactaggt acgttactac catagccaaa ccaaaccatt
     5101 gaaaaaatga caacgaagag aaatcgaata aacaccattt ttgcgtaatg tttccctcta
     5161 aatatacgaa gagtaaatag atataacaca tacaagacga caatccccgc accaatagga
     5221 tcgtatattt ttgaataaat agtatccgaa aaagtgaaaa actggtgaat taactcgttc
     5281 attgcagctc cttcatagag cttctcaatg acaaaatcaa ggccttgcca aatcaattta
     5341 cttaaactaa agaacatatt cgtcatcatt tttagaaaat ctgtgccatt tgccccaaat
     5401 aacattttgc ctaatgaaaa ttcactatca gataaataag aagaaaaggt atcaatatga
     5461 aatttcccaa tctcatagat cgattgatcc acctgctttt ctgccgcata caccaatcca
     5521 ccaacaaaaa gcagtccgaa tccaatcgtt aaaaaaaaca aaacgcacca tttcttttcc
     5581 ttatgcttta tataagccat tgtttcacct catttctatt gcatgattaa cgtatcctgt
     5641 tcttctactt gagagtgact tgttttttcc gttgtttgta atgcagcggt catttcttca
     5701 aacaaactgt gtacattgag ttttcctaca cgtccatagg gatctaacat ccaacactgt
     5761 cctttaatca tgtggctcat ttcagctaag ttcgcttcat ttacttccaa acgtaaatga
     5821 ttcaaaatct tttctcgctc actgtcttca tcaaaagcaa agacgacacc aaaattccct
     5881 ttttctttat ctgaacctaa gtcaccaata aattgtgtat ctaacactaa ttgattcatc
     5941 tgggaacgcc ccacacgtgc cattgctgca ataatactac gtcctgcgtt cgaattatta
     6001 aatacccatg cttctgtaaa ataaatcgct gttttcttac tactatctcg ttttccaaac
     6061 atttcacaaa aacgacctaa actaatcatc aaacacaaag actttcgatc cgcatctgta
     6121 tatagttctg gtcggacatt gtcatttggt aatttcaaat cttggatctc taaaatcgtt
     6181 gttttggcat taaagtctaa tcccgggttt tctccataag aaaatcctaa acgtaaaatc
     6241 gaatcttcta ccgtcaagcg taaaaactca ccatattctc gaacctcttt ttgttccatt
     6301 tgttctaaat agtcgaccac atgcatcaag ccaacttgtt gtccggcttc tttttgaact
     6361 aaaacttctt ggattccctt taataccgcc ccttttaaaa ggtgattggt acctaacgga
     6421 cttaactcat caatcatatc ctgggctact tgtttcgcat ctactcggga taaacaaaca
     6481 attggatcta atacaccata attacgggga tttgtagcat ctaacgtgac ataactcatt
     6541 ttttgcaaat gttctacaaa caaagggtaa tgtttctgat aatacgggtc ttgaatgagt
     6601 ttcgtaaacc atttgcgctt ttctcctttg ggatccacaa ataaactttg tacattcata
     6661 aacgaactat acaaaaagag caagccaact aaaaaggtct tccctttacc ggtttttcca
     6721 gttactgcta catgaggcgc atcaaacaac gcacctttga ttccttccgc aatggctaga
     6781 agattcaaaa agacaaaacg atttgacgca taaattaatt cttctaatgg tgttttttct
     6841 cctcgagaac caccatcccc taatgtatct tgccgaccaa tataccagcc ggattccata
     6901 cctaattctg atactgctga aaacaaccat tcgccaactg cttctacagt tgttcggtgc
     6961 ttccaatgta actgccgtcc caatggttgc cctaataaac atttatagaa caattctcgt
     7021 tgatcatttt ttgcacggtg gatatggata atcgtatgaa gttgttgaat aaccgttgca
     7081 ctattttgac gaacttcttc aaccgtttga ccgtatacaa taaaacatgc gatccaatcg
     7141 acgacaggtt gcccattatc tactcgttgc tttaaatgtt ttactaatcc tcgaacagtt
     7201 aataagcgtt cactaggttc ttcatcattt tgaaccaatt ccttatgctc tgttttgaac
     7261 cgttccgata gccaattcag ttttaaacca atacctccct gtcctttcaa gcgtggatag
     7321 tgaattttca ttcgcaattc tgctgggaaa gaaagatcct gagcaaattg aaatagatca
     7381 caatttctca tattttcagg tgtttcccca ataggcaaca atgctaaata actactccct
     7441 aactcatctt ctaattgaag aaggccttta tgttccttcg gtatacgtac atacgttcct
     7501 tgattgtctt tcagtacttc ccaattattt tgttcttcta ctttgtgagc cattcctcga
     7561 atatattgca gacgattgag atacaacagc tcttttttta ctaagggacg aatagaaaag
     7621 ctataaagta attgtgtcac ttcttttaca taaggatcac tttcagaaaa caaagtttcc
     7681 aaatcatcta cttctaatcc aaataagcgt gctaaacttc ctgctaaatc taaccagatt
     7741 agatttatta catcatgaaa actctccacc gttgcttttt ctttgacttt gattcctaaa
     7801 ataaatgaag gacgatagac ttgaccttct cgttccaaca atgtcactgt ttcttttgca
     7861 taatacgcac caattgcttg actatcggga gaaaatccct ttcctaatgt gtcaaacttc
     7921 gtccgtaaat tcattgttcg tggatacatc accaactcca catcttcata atctgctaaa
     7981 agcaacagta aacgagccat catttctttt atttcctgtg tcttttttgg tgtgtttttt
     8041 gaaattgttc gtggaaaaac atgatagtag gcccagatac aatcctctcc atttaacaaa
     8101 caatttttct tgaatgcttt tactggttct tttacaagca ccaatgaatt tcacctccca
     8161 ataaaaaagg aacaaccagt tgattgtccc tttttctaat cctctaaata aacatactta
     8221 ttcacaaaaa gtaattcttc caatgcattg tttgatacct tgtcccagtt ataatacaca
     8281 caatcaccat tcatcatacg aatcatataa caaaaccaat cccataaata aaaatgcact
     8341 ttttttccat caggttttag ttttgccagt gctaacacac ataaataggg gagtcctata
     8401 taaaaaacaa ctcctgctgg tggaaaaaaa gaaatgaccc aaccaataaa tgaacgtaaa
     8461 aataaatatt caaagattac gaccaacaaa aaagttgctg cattgactaa tgcaactgga
     8521 aatggaagac gccaattgct tgtaatctgt tgtacgcgat aaggttccgc ataaacaatc
     8581 gaataatcat aaggttccca atccacttca ctatcctcct aatccataaa taatttttca
     8641 atgattttac caaacgcatc aaatacacgt gttggatcat ttaagaagaa ataaactgcg
     8701 gcacctgctg cgatagatat taatgcattt cctacttttg atttcataaa atttcgcaat
     8761 cccagataaa caaccaccat tattaaaaaa tagccacctt gcgttaatat aaaatctaca
     8821 attcccttca gtgtcatttc cgttcttctc ctttcctatc caaccaatta tgtgttaatt
     8881 tttcaacata atactgattt gtttttttcg taatgacaat cgtcatttgt tctttttgta
     8941 aaagtgacgt atcaccatct cgaaagacga ccgttgcttt tacaataaaa ggttttttcg
     9001 tttctttata acgcaatact ttcatctcta cttgatcaac tttagctgtt tgattgagtc
     9061 cttcaggttc tttcatcatg tatgacatat cttttgcatt gtttttcgca tacttatcta
     9121 agaaatcttc cacaaatcct tcaacatctt tcgcttccga cgcatcaacc gtttcgtata
     9181 atgtgtcatc ataagtaatc attcgttgtt tttctgtatt ctttggtagt gccgtaaaat
     9241 aaggataagc aataatacga cagcccttgt ttgaaataga aaagggaaga ttgagtactt
     9301 gtgtgattgt cacacttgtt tctttcttac tctctggatt catcgagcga atatcataag
     9361 ttactttata actagcagtt ggaatggttt caatttcttt taatccaata aacgaagcag
     9421 cagttaattt tcgaacacct gaaacacttc ctggttcttg aataaag
//
LOCUS       DASJTC010000094         9348 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.096, whole genome shotgun sequence.
ACCESSION   DASJTC010000094 DASJTC010000000
VERSION     DASJTC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.096"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(79..747)
                     /locus_tag="U4B19_002307"
     CDS             complement(79..747)
                     /locus_tag="U4B19_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2078004.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            881..1168
                     /locus_tag="U4B19_002308"
     CDS             881..1168
                     /locus_tag="U4B19_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078005.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1584..3122
                     /locus_tag="U4B19_002309"
     CDS             1584..3122
                     /locus_tag="U4B19_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEN2078006.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3119..4084
                     /locus_tag="U4B19_002310"
     CDS             3119..4084
                     /locus_tag="U4B19_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2078007.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4071..5393
                     /locus_tag="U4B19_002311"
     CDS             4071..5393
                     /locus_tag="U4B19_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEN2078008.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5456..5560
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5634..6377
                     /locus_tag="U4B19_002312"
     CDS             5634..6377
                     /locus_tag="U4B19_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2078009.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6392..7384
                     /locus_tag="U4B19_002313"
     CDS             6392..7384
                     /locus_tag="U4B19_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2078010.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7396..8133
                     /locus_tag="U4B19_002314"
     CDS             7396..8133
                     /locus_tag="U4B19_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2078011.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8123..8845
                     /locus_tag="U4B19_002315"
     CDS             8123..8845
                     /locus_tag="U4B19_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEN2078012.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8929..>9348)
                     /locus_tag="U4B19_002316"
     CDS             complement(8929..>9348)
                     /locus_tag="U4B19_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN2078013.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3065 a         1607 c         1970 g         2706 t
ORIGIN      
        1 aagattttac tgaataatat cattttagtt tacataatga tatttctgta aattaaataa
       61 agtagtgaaa aataagaatc agtgctctaa ctgttcaaga ggatcaagtg gttcaaatgt
      121 ttctgtagtc tgttttgctt ccaattgggg caaaacagat ccaatttcca gatacttcac
      181 tttattcaat gtataagata gaatagaaca gctaagtaat gtatacagaa aatgtgtgag
      241 atccaaataa agcaaggaga caactaaaat accaaaagta atcaagaaaa gacttttgcc
      301 aatcttaata tgaaaatatt tttcaatggc ttgataaatg atatctgttc cgcctgtgct
      361 tccgccgaat cgaaagacga gaccgcaacc aatgccagaa agaatacctg tcatcaaact
      421 attgatcagt agactatcta cagcaaaatg tatatgaagg ctctcccata aatgtaaaaa
      481 gaaaactagc gcaaatgttc cgacgatcgt ccggagaaaa gcgtgttttc caaaaagaaa
      541 caatgagata agcaataaag gtaaattaaa aagcagtgaa gtgacagaag gagcgaatcc
      601 aaaaagatta tgcaaaagca aagcaatacc agtaacccca ccatctgcca gcttagatgg
      661 aatattgatt cctgtcacag ctaatccgta gattcctgcg cctaaaatga ttccgcacaa
      721 ttctgtaaat aaatttaatt ttttcatttc tagcctcttt tcttttgaaa attttcagca
      781 acttctttat tatactgact tatactctta cttcaagagg aaaatgaaaa aaaattattt
      841 ttggctgttt ctttttattt atcaaataag gagtggaaaa atgcgagcag cagacttatt
      901 gacaattctt acaaaatcac ccaaaaaaac agagtatcaa ggagtttttt tggctcaaga
      961 acaaaaactg gtgcctgtga caatggtcca gataaataaa gcaaatcaat ttatcttttt
     1021 atttgaacac ggcaaagcac cacttgctat gaaagacatc tttctttttt tgatgaagaa
     1081 ccgtgagaaa gctgtttatt atcagtcaaa agaagaactc attcctcttt atggagtgaa
     1141 agagatagaa aataaagtgg tgatataagt aaaaataaga aaacaagacg tttatgatgt
     1201 caaaacaacc attttttaca tttttggcaa aaaagtgtca tgatacagta taatattcgt
     1261 tgaacaggcc aataaaggtg ttctccccca cgagatttct actttattgg ctgtttcttt
     1321 ttattaaaat aaaagttgtg tgtgtaaaaa agatatagct gattccaagt gacaatttcg
     1381 tatagatttt tatggtattc gtagtgaaaa aacgagaacc aaaaagttga tccgactttg
     1441 ttgcttggat tctttttttg tcagcatatt tagaggtatt tttttctttt ttttgttcgt
     1501 gctgtaaggt taaggcaaga caagttagtt gggaaaaagg aaagaaatgt ttatacttaa
     1561 agaataacag aaaggaaggt cgcatgaata attttataca aacattaagt gaaagaaaag
     1621 gcgagttgct ggaagctgca tttcaacatc tgtccatttc tctcatttct ttgctgattg
     1681 cagcactgat tgctattcct ttagcgattt gggcagccaa tcataaaaaa atggcagaag
     1741 tcttattgca gatcacgggt gtgctccaaa cgattccttc acttgcctta ttaggtttac
     1801 ttatcccatt tgtagggatc ggaactgttc cagcattgat tgcacttgtt atttacgctt
     1861 tgcttccgat tttccaaaac acgtatattg gtctagctga aattgacccg tctattgaag
     1921 aagcagcggt agcatttggt atgtccagaa tgagacgctt gctgaaagta gagcttccca
     1981 tcgcattacc tgtcattatc tcaggtatcc gaactgcctt agttttaatc atcggtacgg
     2041 ctactttggc agcattgatc ggagcaggtg gattagggac atttattttg ttaggtattg
     2101 accgaaatac acctgttttg acattgattg gtgcgatcag ttctgccttg cttgcaatta
     2161 tattcagcgg actgattcgc tggttgcagc ataagaaacc tcgatattcc atcattactt
     2221 tagcagcagt ggtgtgttta tttggcggcg tttctttata tcagtctgcc ttgtttaaaa
     2281 atgaaaccat cacgattgct ggaaaattag gagcggaacc tgaaatcctg atccaaatgt
     2341 ataaagaatt gattgaagac aacacaaaaa cagaagttga attaaaaccg aattttggca
     2401 agacgagttt tttgttcaat gcactggatc aaggtcaaat cgatatctat cctgagttca
     2461 caggtactgt cctagaaagc ttggtgaagg tacctgaagc aatgaaaagt gctgatttga
     2521 caaaagaaaa aacatatgac gaagcagaag aattattgaa taagcaattc gatatgacat
     2581 tattaaaacc aatggcttac caaaatacgt atgccttggc agtcaagaga tcttttgcga
     2641 acgaacatca acttaaaaag atttccgatt tggcagctat ctctgatcag atcaaagcgg
     2701 gattcacact ggaatttatt gatcgttcag atggatataa aggaatccaa gaagcatatg
     2761 gcttgaattt ttcaagtgta caaagcatgg agccaagttt gcgttaccaa gcaatcaata
     2821 atggagatgt caatattgtg gatgcctatt ccacagatag ccagctcaag gagtacgatc
     2881 tagtgactct agaagatgat cgagggttat tcccagctta tcaaggcgca ccgctgatga
     2941 aaaaagattt tgctaaagaa catcctgaaa tcgttaaagc actgaataaa ttaagcggaa
     3001 aaataacaga ggatcaaatg attgatatga attaccaagt caatgtcgaa aagaaaaagc
     3061 cgacagaagt agcacgaatc ttcctgaaaa atgaaggatt agtaaaggag gaagatcagt
     3121 gactaaatta atcgagttca aacatgtgca aaaaaagtat gatggaaagt atgtcatcga
     3181 tgacctaaat ctggcaatca aaaaaggaga aatctttgtt ttggttggtc cttccggtag
     3241 tggaaaaaca actactttga aaatgatcaa tggattatct aaaccatcag caggagacat
     3301 ctacttcaaa ggaaaatcgt taaatgaata taatctgcag aaaatgcggt ggaatatggg
     3361 atatgtgctg cagcaaatcg ctttgttccc gaccatgaca gtcaagcaaa atattgaagt
     3421 catccctgaa atgttaggtt gggaaaagca gaaaagagca gaccgtgtag atgaacttct
     3481 tcaaaaagtt ggcttatctc ctgatattta tcgagatcgt atgcctagag agttgtctgg
     3541 aggagaacag caacggatcg ggatcatccg agctattgct gcaagcccag atgtgatttt
     3601 gatggacgag ccatttagcg cattagatcc gatttcaaga aactcattac aagaattggt
     3661 tttgtcctta cacgaagaat tgggcacgac catcgttttc gttacgcaca atatggaaga
     3721 agcaatcaag ctaggtgatc gtattgcatt tatgaaggat ggagaaatta tccaatgtga
     3781 cacaccagag cagttattga tgaatcctaa aaacgactac gttcggcatt tctttgatga
     3841 accgaaacag acgaaagagt ggcgagtaga agatttagtc gtcaatggat atttcttaaa
     3901 tgaaatacca gaaaatgctc gtcaacaagt ttgctttgat acgcctatga aagaagtata
     3961 ttcgcttctt tcggtgtatc caagcattgt gatagtagaa aagcaaagaa gtattgggtc
     4021 gctgacaagt aaagaaatct ttgctttttt aagtcgagag gaggaaggaa atgaaaacat
     4081 atgatgcaat catcatcggt tcaggtgtga gcggcttaag tgctgcttat ggattgaaag
     4141 aagcaggtaa aacagtacta gtggtcgaag aagatctatg gggcgggact tgtcctaacc
     4201 gtggatgtga tcctaaaaaa gtcttgttaa gtgcagtaga ggcacgtaat cgggtaaaac
     4261 aattgagtgg aaaaggtttt aacgagatcc caactgccaa ctgggaagaa cttcaaaaat
     4321 tcaaacgaac atttacggat ccagtcccag aaagccgaaa aaaacaatta gcagaagctg
     4381 aaatcgatca tctttcagga accgctcgtt ttttggatga ttcatctatt gaagtcaacg
     4441 aagaagtctt ccatgcggat tacttagttt tggcaaccgg gcaaagacct acgatcttac
     4501 cagtggaggg aaaggaatac ttgaaaacta gtgcagactt tttatcattg cctgtactgc
     4561 ctaaagaaat catttttatc ggtggtggtt atattgcttt tgagctagct acgattgcca
     4621 atgctgcagg gagcaaagta accattgtcc atcataatca acggccatta aaagaatttg
     4681 aggcgtcttt ggtagaggaa gccgtccatc aaatggaagc atctggcatt cagtttgctt
     4741 ttggtgttga aacccaaaag atcatttcag aaggtactcg ctatcgttta gtcggaaaag
     4801 agacggaatt agtagcagat atgatttttt gtgcgactgg gagacagccg aatacagaat
     4861 ctttagcact tgagcaagcg aatatcgtct ttgataaaca cggaattgca gtcaatgatt
     4921 atctgcaaac gagcaatcca aaaatttttg cgtgtggaga tattgtttca aggaaaaccc
     4981 caaaattgac accagtcgcc acttttgaag gaaattatgt agctaagcgg ataacggatg
     5041 caacaagcga acctatcaaa tatccgatta tacctacgat cgtctacgcc agtcctaaac
     5101 ttgctgaagt aggagttaca aaaagtcacg cctcttcgtc agatcaagtg gtagagatgg
     5161 atcttaccag ttggttcacc tatcatcgtg tcaatgaacc agttgctaag gctgaattga
     5221 cctttgacca gcaaaattat ttgattggtg ctgcagtgat tagcgagcaa gcagacgaat
     5281 tgatcgatga tttgacactc gtcatcaatc aaaaattgac taaaaaagag ttggatagct
     5341 atatcatggg gtatccgaca ttggcaagtg atctctccta tttattgaaa taatcagcat
     5401 gataactaaa gcgcttgttg ctttttcctt cttgtgctac actttaaaaa atgaatacct
     5461 gtttctgggg atgtcgtaga cgacatgaga gtaagttgtg aacttagacc cattgaacct
     5521 gtcagttaat actggtgaag ggagaaacat agcagacgct tgaagcgggt tactatgtcg
     5581 tgcccatttc aagcgttttt tgttattcat attagaagaa aggaggtaac aaaatgaaga
     5641 aaaatcttta tccagcagtc agtatggcat tcattttaat tatttggcag attgttgtca
     5701 tagtttggaa aatacctgcc tttattttac ccggacctgc tgctgtattt tctgcattag
     5761 cggcagatgc acaagtatta ctttcccatt ctgtcacgac attaaatgaa gcagtgcttg
     5821 gtttggtaat agctagcgtt ctttctttta ttactgcctt ggcaatggat tattggaagt
     5881 ttttagaaat gtctttttat ccgttactag tgatttcaca aatgttgcca atcatggtgt
     5941 tagggccatt acttacccta tggtttggtt tcggtatgct tccaaaagtg atccttgttg
     6001 tcttgataag ctattttccg atcgtagttt ctttttcgga tacattgagg aaagtatcaa
     6061 aagagcaaat catctttttg aaaacgatgg gggcagatac agggaagatt tatcgaatat
     6121 ataaaatacc agcgggttta tccggcttct tttccggttt aaaagtggct gctacttatt
     6181 gtgtcagcgg agcagtcgtt ggagaatggc taagcgctca agcaggctta gggtattaca
     6241 tgatacgcgt aaaaaacagc taccagatcg ataaagtttt tgctgcgatc atttgtgtca
     6301 ttctattgag tttattgctg aatggcgctt gttcattatt gaaaaaaggg tattatcgta
     6361 tgctttatat tttttaagag aaggggatca aatgaaaaaa ttatggcttt tattaccgct
     6421 actgttgctt ttatccgctt gtggcactgc aaaagaaaca agtagcaaac aagaaataaa
     6481 ggatttgaaa aaagtgacac ttgttttgga ttatgtaccg aatactaatc atactggcat
     6541 ctacctagca aaagaaaaag gctattataa agaggctggt ttaaatgtcc aaatcatcga
     6601 accaggagat aattcgacaa gtattggtct agtaggtgcg gacaaagcac aatttggtgt
     6661 aagttatcaa gaagatgtca cttatgctca cgcagatgga cagaatatac cagtcaaagc
     6721 aattgctaca gtgatcaaac ataatacttc tggattcgcc accctatcag acagtaacat
     6781 ccattcacca aaagattttg aaggaaaaac atatgcaggt tggcaaagtc caagtgaaga
     6841 agctgtctta aaagctgtga tggaaaaaga cggcggggac ttttcaaaac tgacaatggt
     6901 aggaagcaat ggagaagggc cagaaagctt aggaaaatcc tcagatatcc aatggtactt
     6961 tgaaggttgg gatatgatca aagcaaaaga agcgggtatt gaactaaact atatcccatt
     7021 gaaagaatta gatgaacggt tagattacta tactccggta attattacga atgatcaact
     7081 gatcaaaagt gatccagaat tggtacagtc ttttatggat gccacgaaaa aaggctatca
     7141 ggaagctatt aaggatccaa atgacagtgc taaactgttg caaaaatatg caaaagaaaa
     7201 tgatcggacg tttttagaag aatcacaagc attcttgtca aaaaattata cggatgatcc
     7261 tgagaattgg ggattgatgg aagaaaaagt ttggcgcaat tatacagctt ttatgcagga
     7321 aaacggattg atcaagcaag atgtaccttc gcaagaacta ttcactaatc agtttataaa
     7381 ataggagtga tagatatgtc aatccaaatc agccacttga atgtagtcat tgaaaacctg
     7441 aaaatattag aagacatcca tttcactatc cctaaagata cctttacatc gattgtcgcg
     7501 ccaagcggag ccggtaagtc aacattgctg aaaactttaa caggagtcca gcctatgact
     7561 tctggaacga tcaaagtaga cgatcaaaaa gtaactggtc tccatacagc atttagttat
     7621 atgccgcaag aagatatgtt acttccttgg ctgagcgtct atcaaaacgt tacgttatat
     7681 caaaaaatca atcatctaac aatcaatgaa gaacaagtaa gggattatct ggatatcttt
     7741 gggttaaacg gctatgaaca ttttttgccg gaacaattat ctggcgggat gaagcagcga
     7801 acggccttgt tacgaacaat catgaatcct tcttcttact tgcttcttga tgaaccattt
     7861 ggagcgttag atgctatgac cagagggcag atgcaagact ggatgttgaa gctgcctaaa
     7921 aaagcaaagc ggactacttt acttgtgacg catgatatcg aagaagcaat ttatttatca
     7981 gacaggattc ttgtgctttc cgcccgtccg gcacatgtga ttgctgagat tcaagtgcct
     8041 gaaaaacgac ggtcacgtga atggctactg cagcaatcag agttgaagca aacgatttat
     8101 cagttattgg caggtgaatt agatgttaag tgatgctcat tgtcatttag atgggagtca
     8161 gagtttagca ctccttcagc aggaacacac gattttgacg atcatcaatt gtgattcgcc
     8221 agaagaatgg aaggaaaaca gacagcttgc agcaagtaag actcaagcct taagttacgg
     8281 tatccatcct tggaaagcag atagctatac gtttgaacaa gttgagcctt ttttgaaaaa
     8341 agcaaggatc atcggtgaaa tagggttaga taatatttgg acgaatgtac cgatgactac
     8401 acaaaaaaaa gtatttgagc gacagcttgc ttttgcagcc ataaatgaga aacctgttgt
     8461 actgcacaca aaaggatgcg aaaaagagat cttaaattat atccaagctt accctaatca
     8521 ctatttgatc cattggtatt cttctttgga ctatcaacaa gactatattg atttaggctg
     8581 ttatttcagc attggtcttg atttgaaaaa gaatccagca gtctggcaac tagctaaagc
     8641 agtcccaatc gaccacttac ttattgaaac ggatggacta catgcagcag agtgggcttt
     8701 aggggggatt ttttcagaaa aagattatgt ctcactgctt gaagaacagt taaagacaat
     8761 cgctgagatc aaagagctat cagttgaaga agttaaaaaa aatacttatc aaaacttgga
     8821 acgtttcatt agattaggta aataaaaaag agatgtgaca caactagtgt cgcacctctt
     8881 tttggctttc tttgtataaa agtaaactgt ttgattaata aatgaatctt agtaccaacc
     8941 gtttgcttcc cagaatgctt tcgccgcttc ccaagaacca taacgtgaag taacatattg
     9001 ttctgctact ctttcttggt ttgcagcaga gtagtcacca tttaagtaag aagaatctaa
     9061 ttggtaacgt ccgatgtaac gtccgttcgt tgctgtgtaa gaaccgcttg attctttttg
     9121 tgcgatccat tcttttgcag agcttgtgct tgctggagta gcagcagttt ctgttacagc
     9181 aggagcaggt tgtgtttcag caacaggcgc tgcttgtttt tccgtttgaa ccgtttcttg
     9241 tacaacaggt tgttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc
     9301 tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DASJTC010000095         9018 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.097, whole genome shotgun sequence.
ACCESSION   DASJTC010000095 DASJTC010000000
VERSION     DASJTC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.097"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1101
                     /locus_tag="U4B19_002317"
     CDS             <1..1101
                     /locus_tag="U4B19_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2078014.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1609)
                     /locus_tag="U4B19_002318"
     CDS             complement(1280..1609)
                     /locus_tag="U4B19_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEN2078015.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1599..1871)
                     /locus_tag="U4B19_002319"
     CDS             complement(1599..1871)
                     /locus_tag="U4B19_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEN2078016.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="U4B19_002320"
     CDS             complement(2322..3125)
                     /locus_tag="U4B19_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEN2078017.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="U4B19_002321"
     CDS             complement(3103..4227)
                     /locus_tag="U4B19_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2078018.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="U4B19_002322"
     CDS             complement(4243..5058)
                     /locus_tag="U4B19_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEN2078019.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="U4B19_002323"
     CDS             complement(5048..5920)
                     /locus_tag="U4B19_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2078020.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="U4B19_002324"
     CDS             complement(6005..7294)
                     /locus_tag="U4B19_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEN2078021.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7522..8166)
                     /locus_tag="U4B19_002325"
                     /pseudo
     CDS             complement(7522..8166)
                     /locus_tag="U4B19_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
     gene            complement(8643..>9018)
                     /locus_tag="U4B19_002326"
     CDS             complement(8643..>9018)
                     /locus_tag="U4B19_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2078022.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVWLSVRTAFFVG"
BASE COUNT         2778 a         1933 c         1427 g         2880 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgttaa tttcttgtaa attccctgcg ttttttcagt aaactagttg
     8281 taaatcttgc atgctccttc ctacgaactc atgaatcgtc gattttgccc ttctagctag
     8341 tttcaccaat taagtagttc tacattcccc gcttttaacc tactacacct ttttttgttt
     8401 tcagctattc cttcttcttg ctgaaagtcc ttgaggttct ttcttacagg ataccatcaa
     8461 ggtgttcaat ggcttattcc cattgaatga ccgagactta ctatcagatt tctgctcgtt
     8521 gaaccaagtc tttgatacag gatttcttcc gtcatcttct cacaaatacc accttgcgtt
     8581 tctctagcga ttggtcgatg tgcaccctcc cagtggactg tcactacccc tttttgctat
     8641 gcctagccca caaaaaaagc tgtcctcaca gacagccaga ctgtagacaa aagctgttcc
     8701 aaactcgtaa ctcgagcttg gaacagcttt ttatctactt cgtttcgaat ttttgaacaa
     8761 aaaaataaag gctccttgcc tttccatagc caggtggcta atttctttaa attcattgca
     8821 gcaaacacta gcatcgtatg cgtcgcgact ttttccaagc ctcgatactt ggtccaacgc
     8881 atcccatgct tttcttttgc atccgcaaat attcgttcaa tcgtttgttt ccgttttttg
     8941 taaattgatt tattcagatc tgttaggcga agatgttcca cttcatccat cagatctccc
     9001 caaacatgtc tcgtgatg
//
LOCUS       DASJTC010000096         8942 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.098, whole genome shotgun sequence.
ACCESSION   DASJTC010000096 DASJTC010000000
VERSION     DASJTC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.098"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(13..1152)
                     /locus_tag="U4B19_002327"
     CDS             complement(13..1152)
                     /locus_tag="U4B19_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEN2078023.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1551..2849)
                     /gene="eno"
                     /locus_tag="U4B19_002328"
     CDS             complement(1551..2849)
                     /gene="eno"
                     /locus_tag="U4B19_002328"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEN2078024.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2965..3720)
                     /locus_tag="U4B19_002329"
     CDS             complement(2965..3720)
                     /locus_tag="U4B19_002329"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEN2078025.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3824..5014)
                     /locus_tag="U4B19_002330"
     CDS             complement(3824..5014)
                     /locus_tag="U4B19_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEN2078026.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5124..6125)
                     /gene="gap"
                     /locus_tag="U4B19_002331"
     CDS             complement(5124..6125)
                     /gene="gap"
                     /locus_tag="U4B19_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEN2078027.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6166..7257)
                     /locus_tag="U4B19_002332"
     CDS             complement(6166..7257)
                     /locus_tag="U4B19_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEN2078028.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="U4B19_002333"
     CDS             complement(7406..8740)
                     /gene="rpoN"
                     /locus_tag="U4B19_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEN2078029.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8800..8871
                     /locus_tag="U4B19_002334"
     tRNA            8800..8871
                     /locus_tag="U4B19_002334"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8833..8835,aa:Arg,seq:ccg)
BASE COUNT         2318 a         1897 c         1648 g         3079 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgcccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata tttcagcaag tagagagttc gattacttaa ctaagtgatc
     1501 atactcgact atctttcaat agtcgatcat gtatcttcat tagaaaagaa ttatttgttt
     1561 tttaagttgt agaaagattt caaacctttg tattctgcaa cttcaccaag ttggtcttcg
     1621 atacgtagta attggttgta tttagcaata cggtcagtac gtgatagaga accagttttg
     1681 atttggccag cgtttgttgc aacagcgata tcagagattg ttgaatcttc tgtttcacca
     1741 gaacggtgag atacaactgc tgtgtagcca gcttctttag ccatttcgat agcttcgaaa
     1801 gtttctgtta atgtaccgat ttggttcact ttgataagga ttgagttagc aattcctttt
     1861 tcgatacctt cagataattt ttgagtgttt gttacgaaca agtcgtcacc aactaattga
     1921 actttgtcgc ctaatacgtc agttaatttc ttgaatccgt cccagtcgtt ttcgtctaat
     1981 ccatcttcga ttgagatgat tggatattta gaaactaatt cttcgtagaa tttgatcatt
     2041 tcgtcagttg ttttttcgcc ttcgcctgag tcagctagta cgtaaacacc tttttctttg
     2101 tcgtagaatt ctgaagaagc agcatccata gcaagaacaa cgtctttacc aggtacatag
     2161 ccagcttttt cgattgcttc gatgattact tcaaaacctt cttcgtttga accaaggttt
     2221 ggagcaaatc caccttcgtc accaacagaa gttgctaatc cgcgagcttt caagattgaa
     2281 gctaatgcgt ggaatacttc agcacccata cgtagagctt ctttgaatgt tggagcgcca
     2341 acaggcatga tcatgaattc ttggaagtcg attgagttat cagcatgtga tccgccgttg
     2401 atgatgttca tcattggtgt tggcaatact tttgtgttga atccgcctag gtagtggtat
     2461 aaaggtactt ctaggtagtc agcagctgca cgtgcaacag cgatagaaac acctaaaata
     2521 gcgttcgcac ctaatttacc tttgttagga gttccgtcta aagcgatcat agctttgtcg
     2581 atagccattt ggtcacgaac atcgtagcca atgattgctt cagcaatgat gttgtttacg
     2641 ttgtcgacag ctttagttac acctttaccg ccataacgag ctttgtctcc gtcacgtaat
     2701 tcaactgctt cgtattcacc agttgaagca cctgaaggaa ccattccgcg gccaaaagct
     2761 ccgctttctg tgtatacttc tacttcgatt gttgggttac cacgtgagtc taagatctcg
     2821 cgagcgtaaa catcagtaat aattgacatg ttttgtctct cctttgagtt tgttttttcc
     2881 taagggattt ccctcatata cgattttagt taattttgct cattagtgca aacatttctt
     2941 gcatttttcg caaattttca acaattattt tacagcgtct aacaatgaca agaatgaatc
     3001 tgcttgcaag cttgcgccac cgactaaagc accgtcaaca ttttcttttg ccatgtattc
     3061 agcaatattt tctggtttta cagaaccgcc gtattggata cgaactgctt ctgatacttc
     3121 ttttccataa agtttttcaa ctgtttgacg tacaactcca caaatttcgt cagcgatttg
     3181 tgcgtcagct gatttaccag taccgattgc ccagattggt tcataagcaa taaccatgtt
     3241 tgacacctgt tcagctgaaa gatcagctaa accagctgta atttgtcctt cgatccattc
     3301 agcagttttg cctgcttcat aggtttctaa agattcgccg cagcatagga ttggtgtcat
     3361 accatttgca aagatagctt tcgctttttt attgatgtct tcgtctgttt catggaaata
     3421 ttcacgacgt tctgaatgtc cgatgatcac ataatgaaca cctagatcag caagagctgc
     3481 tggtgaagtt tcaccagtga acgcacctga gttttcccag tagcagtttt gtgctgagat
     3541 ttttagttct gtaccttcag ctgatgtcat taattcttgt aagaataatg caggagatcc
     3601 gatgactgaa tcaactttgt catttgaagg gattttgttt tttactgctt cagcaaattc
     3661 ttttgcttct gatgcagttt tgttcatttt ccagttacct gcgataatag gtttacgcat
     3721 tgaggaacac actccttaat ttattgaaaa atggctaggg agtcatgaaa actcccttca
     3781 ccttttgttc gattactcgt cagaattgag caaacttggt cgtttattta tcgttgattg
     3841 ctgctagtcc tggtaattct ttaccttcaa gcaattccaa gcttgcgccg ccacctgttg
     3901 agatgtgtgt gaatttgtca gcaaatccta attgttctgc tgctgcggct gagtctccgc
     3961 caccgatgat tgttgttgcg ccttctaagt tagcgattgc ttcacaaaca ccgattgtac
     4021 ctttagcaaa gttactcatt tcaaatacac ccataggtcc gttccaaaca actgtttttg
     4081 caccttgtaa tgtatcagca aataatttta ctgatgctgg gccgatgtct aaagccatca
     4141 aaccatcagg aatgtctcct tcgattactt gtgtttcaac atcatttgaa aattctggtg
     4201 cacagacatt gtcgattggc aagacaagtt tgtcgcctgc tttttcgatt aattcttttg
     4261 ctagttccac tttatcagct tctactagag agttaccgat cttgattcct tttgcttcgt
     4321 agaatgtgta agtcattccg ccaccgatca agattttatc tgctttagga atcaagtttt
     4381 cgattacgcc gattttatca gaaacttttg cgccaccaag gattgcaatc attggtcgtt
     4441 ttggttcttc tactgcttca ccgatgaact tgatttcttt ttccatcaag aatccagcaa
     4501 ctgttgggat accagttgaa gcgataccta cgttagaagc atgtgcacgg tgagctgtac
     4561 cgaaagcatc gttaacaaat acatcgccta atgaagccca gtatttacct agttctgtat
     4621 catttttgct ttctttttta ccgtcaatat cttcaaatcg tgtattttcg aatacgacaa
     4681 cgtcgccgtc attcatgttg cggattgctt cttctaattg ttctccgcga gtttcaggta
     4741 cgaatgttac ttctttacct aataattcgc ctagacgttt tgcaactgga gctaaagatt
     4801 ttccttcttt atcttcttct gtttttacac gtccaaggtg agagaaaagg attgcttttc
     4861 cgccttgttc tagaacgtag ttgattgttg gcaatgctgc tttgatacga gtgtcgtcag
     4921 tgatcacgcc gtctttcaaa ggaacgttga agtcaacacg gacaagaact tttttgtctt
     4981 ttaaatcgat atctttcact gttttcttag ccattttatt tcctcctgaa taaacttatt
     5041 atcaaaaaaa gcggaagaag cgcgattgct tccccgcttt taaatgtaca tgtctataat
     5101 ttacacttgt acgtttgtct atcttataag ttagcgaagt attctaaagt acgtactaat
     5161 tgtgcagtgt atgacatttc gttgtcgtac caagcaacag ttttaactaa ttgtttgtcg
     5221 ccaactgtca ttactttagt ttgtgtagca tcaaataatg aaccgtaagt cataccaacg
     5281 atatctgaag aaacgatttc gtctgtgttg tatccgtaag attcgtttga agcttctttc
     5341 attgctgcat tgatttcgtc aacagttact tctttttcaa gaactgttac taattctgtt
     5401 aatgaacctg ttggtacagg aacacgttga gcagctccgt ctaatttacc gttcaattcg
     5461 ggaattacta aaccgatagc tttagcagca cctgttgagt taggaacgat gtttgcagca
     5521 gcagcgcgtg cacggcggaa gtcaccttta ggatgaggtc cgtctagagt catttggtca
     5581 cctgtgtaag cgtggatagt tgtcattaat ccttcaacaa caccaaattt gtcgttcaat
     5641 gttttagcca taggagctaa acagttagta gtacatgaag cacctgagat aactgtttct
     5701 ttaccagtta atgtttcgtg gtttgtgtta taaacgattg ttggaacatc gtttccgcca
     5761 ggagcagaga taacaacgcg tttagcacca gctgttaaat gtttttcagc tgctgattta
     5821 gaagtgaaga aaccagtaca ttctagaacg atatctacgc ctagttcgcc ccatggtaat
     5881 tcttctgggt tgcggttagc taatacttta acttcttttc cgtttacgtt gaatgaacct
     5941 tcatgaactt caactgttcc gttgaaacgt ccttgagttg tgtcgtattt taacaagtgt
     6001 gccaacattt tagcatctgt caagtcgttg attgctacta cttcgattcc atctacatct
     6061 tggatacgac ggaatgctag acgtccaata cgtccaaaac cattaatacc tactttaact
     6121 gtcatttaag atttcctcct tatgaaaatc aaaaaaatat ttattttaaa gggttacccc
     6181 ttttaaaatc tcatttgcag cagcttcatc agtgatgagc cacgtttgtt ttggtgcatt
     6241 tttcatataa gctcgaattg ctttagcttt tgattttccg cccgcgattg ccatgatgat
     6301 cgggactttt tccaaatctt gcaattctat gccgattcta gggaccttat aaacgacttc
     6361 acctttttca ttgaaaaagt agccaaaaga ttccgcaaca gcatttgctt gtttcagcat
     6421 gactaattct ttttcagtca tcttacgacg tgctgccatg tgtaatgcac gcccaatact
     6481 atgaataaca caattcgctt cactaatcaa gtttaagact tcttggatag atggctcatt
     6541 gagtaaagaa ttgtaagttg ccagacttaa ttgttccgga acataaagtg cgcggtggtt
     6601 accgtttgct ttagcagcca ttcttgcact gacagaattt gcctgtacag ttatcgcttc
     6661 accaatcccg cctcttgcag ggacaaacag gttatgccgt ttcttatttt ccagattaga
     6721 caattgttcg gctaccacag ccatagtagt tcctcccatc acagcgatga tattctcgcc
     6781 ttctggaagt agtcggtcta aggcttcgct cagcgcttcg ccgaatgcat cagccacttt
     6841 ggtctgctct tcgctgtttc ctgaaacaac gatacagcgt tggttaccaa aatattctgc
     6901 caactgttgt tcggtttgat gcgtacctaa aagctgatcc atgaagtttt caaggctttg
     6961 ataaacttct tcgcccttag ttgtcagcgt cataccgctt tttgaggtgt cgatcaaatt
     7021 cagatttttc aaaaggtctg tttctgttcg taagactcgt tctgtgatcc ccatactttc
     7081 cgataaactt cgacgcccaa tcggctgcat ccaatagata tttcgtaaaa ttttatagcg
     7141 ttcttgcaac acttcgataa tatctggtgc aacagattca atcattttta tttcatccag
     7201 catagcgatc tccttagttg gacactttac gtccaatgta gacttaaaac gacccatata
     7261 cgataaaaaa agaaattaac tattcgaatt tcacatcaat aaagggaaat ctcaacctca
     7321 cgactgtaag tctaacagtt ttgctctcgt ttttcaacta aaaagactag aaaaagtgaa
     7381 atactttttc tagtcttttg actgattagt tatcataacg ttttcttttt gatgaactcg
     7441 ggattcctaa taaatcacga tattttgcta ctgttcgtct tgagatcgct atctcatctt
     7501 ttttcagcaa ttcaaccaat ttctggtcag acaacggctt gtttttgtct tcttgatcga
     7561 ctaattcctg cagctttttc ttcgctgtat ctgctgaaag gtcttctgtc tgttctgtac
     7621 tattcgtagg aattcgtgta gtgaaaaatt tttttaattc aaatacaccg aagtctgttt
     7681 ccaaatattt cccgttgact gcacgactga ccgtggattc atgcacatct atctcagttg
     7741 caacttcctt taacgtaaga ggcttgattg gacgttcctt gtcgataaag aaagcttttt
     7801 gccgggaaac aatgacttga gcaacccgca aaattgtatc cccacgttgt aggatcgttt
     7861 tcttcaacca ttcgaattcc tgtttccgtt cttttaaata attttcagtt tctttatctg
     7921 cttgttgtga catttgtttg aagtaactct cttgaaagcg tatatttggc tgattttttc
     7981 ggtttgagat aacttgtaca tttttatcgt cgtccactaa tacccggaca tcaggaataa
     8041 tgtacaattc gctgctacgg tcgaaaatcc ggccagggct tggactcaac gtttgtaaat
     8101 agtcgaatat ctgctgaaca gcatgtagat caacagaaaa acgttgtgcg atttctttcc
     8161 attttcgctc caccagttcc tcaaaacatt cttccaacac gatataagct aattccggcg
     8221 cataatcatc tcgttctgtt tgaagcatga gacattcttg caaactacgt gcacctactc
     8281 cagctggttc taattgctga atcagagtca acgcatcgag catttggata ggcgtagctc
     8341 caatctcttt tccggcttct tctaaactaa tcgtcaaaaa tccgttcaaa tcgatgtatt
     8401 ctacaagata aagaacgatt ttcctcaaaa aagtatctcg gtaattcaaa tggacttgtt
     8461 cgatcaatga atcaaataaa gacccttttg tatcaggaat ctgatttagg taatttgtct
     8521 cttctccttc ataagaagat gaagaagctt ttatgtaatc tggctgccat tccggctcaa
     8581 caacttccac caatggattt tccattgctt gattctcaac gaatgccaac agctcctcac
     8641 tgttatattg taaaacttgg atggactgtt gcaactgttg cgtcatggct agtttctgtg
     8701 tctgctgctg tttttgtgaa aaattttgct caaatttcat ttttttgtcc aatccccttg
     8761 taatttttta tctgtttagt tacaattatc ccagtgcttg cgtccttagt gtagtggata
     8821 tcacgtaaga ttccggttct tgagacgggg gttcgattcc ctcaggacgc gttctactgc
     8881 tattttagca taatttacga atagttgctt ctttattttt aagtgaaaac tgtccgccaa
     8941 ag
//
LOCUS       DASJTC010000097         8849 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.099, whole genome shotgun sequence.
ACCESSION   DASJTC010000097 DASJTC010000000
VERSION     DASJTC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.099"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            60..1121
                     /locus_tag="U4B19_002335"
     CDS             60..1121
                     /locus_tag="U4B19_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEN2078030.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            1124..2020
                     /gene="murQ"
                     /locus_tag="U4B19_002336"
     CDS             1124..2020
                     /gene="murQ"
                     /locus_tag="U4B19_002336"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEN2078031.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            2034..4016
                     /locus_tag="U4B19_002337"
     CDS             2034..4016
                     /locus_tag="U4B19_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2078032.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(4081..4527)
                     /gene="nrdI"
                     /locus_tag="U4B19_002338"
     CDS             complement(4081..4527)
                     /gene="nrdI"
                     /locus_tag="U4B19_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEN2078033.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            4738..6162
                     /locus_tag="U4B19_002339"
     CDS             4738..6162
                     /locus_tag="U4B19_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2078034.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(6343..7116)
                     /locus_tag="U4B19_002340"
     CDS             complement(6343..7116)
                     /locus_tag="U4B19_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEN2078035.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(7113..8018)
                     /locus_tag="U4B19_002341"
     CDS             complement(7113..8018)
                     /locus_tag="U4B19_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2078036.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(8172..8492)
                     /locus_tag="U4B19_002342"
     CDS             complement(8172..8492)
                     /locus_tag="U4B19_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEN2078037.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         2784 a         1587 c         1880 g         2598 t
ORIGIN      
        1 tctttagata tatactctag tcatagaaat gaagacggat acagaaaaag agggataata
       61 tgtttggctt ttcaatattt atgaacgatt ctttgtcaga agagaagaaa cagtatatcc
      121 gccaaatggc atccaatggc tttgtcggta ttttcacttc catgcatata ccagaagatg
      181 atgtttcggc atatcaaaag cgattgatcg aactaggaag cgaagcgaaa aagaataaac
      241 tagaattgat ggtcgatatt tcaggagagg cattggaacg agcgggtttt tcattagaag
      301 atctaacgcc tttgaaagca attggtgtaa caggtttaag aatggattat catatttcca
      361 atcagcagat tgccgactgg tcacatcaat tgaaaatcag tttgaatgct agcacgatca
      421 cgcctaaaga tatcgatgaa ttgaaagaag cagaagctga tttctcacag attgaagctt
      481 ggcataatta ttatccacgt cccgaaacag ggctggacaa agaatggtat cagaaaaaaa
      541 atcaatggct caaaattcaa ggtctccttg tccaaggttt tgtaccagga gatacagagt
      601 tgagaggccc actatatcaa ggattgccaa ctttggaaga acatcgaggg atccatcctc
      661 ttgcaggcgc tctagatcta ttagcaagta acacggatat tgtttacatc ggagatgcag
      721 gtctgtcaga aaacgttcag gagcaatttg cttctttcca aaaagaacag acagtcttgc
      781 tccatacgga acctgtagat gaagaatttt acgaatatat attagggaag catacgaatc
      841 ggcaagacga tgcaagagat gtcatacgaa gcgtagatgc acgttttcga gagattccgc
      901 ctatcccagc aagaaataca gctacgcgta tgaaagggtc tattacttta gataacgaaa
      961 agtatttgcg ctatatgggt gagatccaat taaccaaata tgatttgcca gcggatgaaa
     1021 aagtgaatgt agtagctaaa gttatcaaag aagagctacc attgatcaac caaatcaaag
     1081 ctggtatgaa ctatcaattt ataagaaaag aaggaagata aaaatgagca aaatcaattt
     1141 agagaaccta acaacggaac gcagaaacca agaaactttt ggattagatg agatgagtat
     1201 ttctcaagcc gttcaactaa tgaacaaaga agatcagaaa gtggccgctg cggttgaaaa
     1261 agaactgaca agtatcgaac cagtgattgc taacacgatt gagtccttta aaaaaggtgg
     1321 acgtctgatt tatatgggag ccggaacaag tggaagacta ggtgtgttag atgctgctga
     1381 atgcgtaccg acattcggtg tagaaccgga aatggtcata ggtctaattg ctggtggaga
     1441 agaagcaatg actgtagctg tcgaaggggc cgaagattct tatgagctag ggcaagaaga
     1501 tctaatcaag ctgaatttga caaaaaatga tatagttatt ggtatcgcag ctagcggtcg
     1561 tacaccatat gtgatcggtg gattagatta cgcaaaatcg atcggtgctg tcacaggaac
     1621 gatttcttgc aataaacaag ccgaaatcag taaacacgcg gatttcccaa tcgaagtcga
     1681 tgcaggtcca gaatttttga caggctctac tcgattgaag tcaggaacag cacaaaaact
     1741 tatcttgaat atgatttcta ctatcagtat gattggtata ggtaaagtat tcaataactt
     1801 gatggtcgat gtcaaaccaa caaatgaaaa actaatcgaa cgctcaaaac gaatcatcat
     1861 gcaagcgaca gaagctgatt acgaaacagc tgaaaagtac tttgaagaag cagatcaaaa
     1921 tgttaagctg gctatcgtca tgattttgac aaatagtgat aaagaggaag ctcaagcaaa
     1981 attagctgaa gcaaacggat ttgtaaaagg gaccttatga gggggaaaag accatggcaa
     2041 aaaacgaact gactttggaa gaactggcaa aacaaattta taaaggggca ggcggcatgg
     2101 gcaatgtaga aagcattgtc cactgcatga ctcgtgtgcg tatgaagatc atcaatgatg
     2161 atcttgtaaa tgtctctgaa ttaaaagcga ttccaggagt gcttggtgta gtagaagatg
     2221 aacagcttca agtcatcatc ggacctggga aagtgaacaa agtagcacag caaatggttg
     2281 atttggcagg cgtgaaatta ggagaaaaac tttcttctgt aagtacagct acagcaaatt
     2341 catcagcatc tggaaaagac cgtgtaaacg aacgggcaca agaaatgaaa gcggcacaaa
     2401 aatcgaaaca aaaaccatct aaaatcaaat cggtattaaa agacatttct aatatctttg
     2461 tgccaatgat tcctgcattt gtcggtactg ggatcgtggc agggattgcc gcagttttag
     2521 caaacttagt cacagccgga acacttgatg cagcaacatg gcaacaatat atcgatgtga
     2581 tgaacatctt gaaaaacggg atgtttagct acctagttat ctatacaggt atcaatgctg
     2641 cacaagtttt tggtgcaaca ccaactcttg gtggagttat cggggccgtt gtgatgctga
     2701 cagggatgaa tccggaagca ccactgaaaa acttatttac tggtgaagct cttgcagctg
     2761 gacaaggtgg gatcctaggt gtcatcttcg ctgtttggct attgtcaatc gttgagaaaa
     2821 aactgcataa agtagtacct gatgcggtag atatcatcgt tacaccaact ctttcattga
     2881 ttgccatcgg tctgattgaa attttcttca tcatgccttt agcaggtttc atttctgatg
     2941 gcatggtcgg tgggatcaac tgggtacttg gcgttggtgg agcatttgcc ggcttcgttc
     3001 taggcacatt attcttgccg atggttatgt tcggactgca tcaaattttg actccaatcc
     3061 atgtgcaaat gattgatgaa actggtcgta cgctattgct tccaattctt gcgatggctg
     3121 gtgcaggaca agtgggtgca gcattggcat tatggattcg ctgcaaaaaa gataaagaat
     3181 taacagaaat gatcaaagga gctttaccag tcgggatcct aggaatcggc gaaccattga
     3241 tttacggagt aacattacct ctaggacgcc catttattac tgcttgtatc ggtggaggta
     3301 tcggcggggc agtgatcgga atgatcggaa atgtcggggc tatcgctatt ggaccttcag
     3361 gagccgcttt gatcccattg atctctgacg gtaaatggtt aggctacatc cttggattat
     3421 tggcagctta tgctggtggc ttcgttgcta cattcttctt cggtattcct aaagaccgtt
     3481 tagaaaaaga agaattagcg actgaaacag ccacagtaaa tgaagtgcct gaaaaagtag
     3541 tacctcaaac ttcagctgaa agtgtgacta tcctctcatc agttgctgac ggaacagttg
     3601 tttctttaga taaagcaagt gatccagttt ttgcacaaaa gatgatgggt gaaggctact
     3661 ttgtagaacc agaaaacgga catatctatt ctccagttac tggggtagtc agctctgtct
     3721 tcccaacgaa acacgctatt gggatcacaa cgccaaatgg tttagaaatc ttgcttcata
     3781 tgggaatcaa cacagttgac ttaggcggta cgccatttga tgtgaaagta acagaagggc
     3841 agcaagtgac acctgatacg cttgtagcag aagctgactt agaagcgatc aaagcagcag
     3901 gcaaagaaac atccatgatc gtcttggtaa ccaatatgga tcgtgtaaaa aactttgtct
     3961 tggaaaaaac aggtaaagca aaagcaaaag cacctgtgat ggatgtcaaa gcttaatatg
     4021 atagaaacaa tagaaacgaa agaagaaact gaaatctatt tttcagcttc ttctttttta
     4081 ttatttattg ttttctgcta aaatctgata aattcgtgtc acatcagatg gtgttccccg
     4141 taattcataa tctgccaaga aaggaaagcc aaatgtttgt gcatattgtt tggctgttag
     4201 acaatactga ttattgaagt ttttatttcc gctaccgact acaccaagac aaagtttatg
     4261 attgtcttca tgttccagat attcacgcat cgtttcggtc aagatttctg tatcaccgtt
     4321 atctaaacca tttccgccgt ctaaataagt aggaacgaaa gtaaaaaatg gttctgtttc
     4381 tttcgtaaaa tcactatttt catggatttc ttttaaagta acttcaggat tgacaggatc
     4441 ttctgcatgc attttttcag catattctgc tagatgtttg gcaaaggcac gcgtatttcc
     4501 tgaaatcgat atatataaaa tgttcatgga tcatcctcca attctttcct tttagtatag
     4561 catctttttt aagttttttg gtggtgtaat ttggaaaggg actaggcgct ttgcttgtat
     4621 aatgagaaaa aagccggtag aattatattt ctattaaagt ttttattaat taagtaaaaa
     4681 taaaaaaggt ttgttatact ttttgataaa agcggtttta ttttggagga tgcttacatg
     4741 gatacagagc aacagaaaaa aggaattggt ttaattccac ttgctgcctt agtaattggt
     4801 tcagcgattg gaggtggcgt atttggtatt atgacggaca tctccggatc ggctggcggc
     4861 ccagctattt tcagttgggt actcgtagga acagcaatgt tgatgctctc cttgtcgatc
     4921 aataatatca ataaaaaacg tccagattta aaaggcggga tctttagtta cgcagaagaa
     4981 ggttttggcc gtttcgctgg ttttatcagc ggttggggtt actggctcac atgctggtta
     5041 ggcaacgtag cttttgcaac attgttgatg agcgcattag gatatttctt cccagtattt
     5101 gaaggtgggc aaaatctggc atcgatcgtg atgagtacag tctttttatg gggatatgca
     5161 tggctagtga atcggggagt cgaaaatgct agtttcgtca atgctatcgt cacattctgt
     5221 aaattagtgc cattattttt atttattgta gtagctatca gcacgttttc gatccaacaa
     5281 ttcattgata acttttctag cgacattata ttgaatgcga atggacagcc gatcccgttt
     5341 tccagtcaag tattgagttg catcatggtg atgctttggg tatttgttgg tattgaagga
     5401 gcctctgtcg taggttcacg agcaaaaaaa caatcagatg ttgggaaagc aacgattcta
     5461 gggatattcg gcttgattat tatttacgtc ttggtttcca tgcttccata tgggacaatg
     5521 acgatgaatc agctgcaaac gatcaaggcg cagcctgcta tgggctatgt gtttgaaatg
     5581 atggttggta aatggggagc agtcgtgatc aacggcggtt tgatcatttc tttagttggt
     5641 gtttggctct catggacgat cttaccgatt gaaacaatgg gaaacatggc tgaagacgga
     5701 cttttgccta aagcatgggg aaaactcaac aaaaatgggg caccaactta tgctatcgta
     5761 ttgacgacgt tatgtacgaa tgtcttttta ctttcattat tattcactga cagtgcttat
     5821 aactttgctt acacacttgg tacagcagct attttcttca cgtggctttt cttaggattg
     5881 taccaaatga aactttctta tcagcggaaa gaatgggtcc aatttttagt agggctttta
     5941 gcaagtgcct ttcaagtatg ggctatgcta gtcgtagcct ttaatgaagt aatgcttgtt
     6001 cttgttcttt tcttgccagg catttatttt tacctcaaag cacgtaagga gcaaaacgag
     6061 cctgtacaac atatccactt agataagatt ttacttatag gtgtgacgat ggtcggagtg
     6121 attgcaatgg ttttatttgc cactgggatg cttcacgtat agaagtataa aagaagtata
     6181 aagagaggtt gtgagcctgt cgtttattca aaaacatact aggaaaggag ctgtgacaca
     6241 aatgttgtca cagctccttt ttcagaataa acggtgttta agagacagcc aatcggcaat
     6301 aaggctgaac gtctctttca cagcctcttt tttgatgagt catcatacat tttttcgctt
     6361 aaatacgaga tatcctaatg ctaagaacaa aacgatgtaa gctaaattac cgaatagcat
     6421 ttgattagta gaaagatgag tcaattgctt catgatctct tcgtttccaa tttggttttg
     6481 cagattcaac atactgatcg gattccattt gatccactcc catttttgga tgagtgcaat
     6541 aaggatacca gatatgattg acgaagcaaa ataaaagacg attcctgctg agatggatgc
     6601 gccggaacta ttgataaagc aagcaatcat caacactaaa cttaaaatca gccataaacc
     6661 gatataacta cccaaagtat aagtgaacaa gctttggatc agtgtttgac cagtagaaac
     6721 cttctccgtg aaggaaatcg aagggaaaag aatcagtttc atcaacacag tgacaataat
     6781 agctaaaagg tataaataaa cagaataaat aactaatgtg atccatttgc ttactaatat
     6841 ttctcctcga gaatactttc gataaaggag atttttcaat gtaccatatt gtgcttccat
     6901 cgaaatgatc gtgcttgcag ctgcaatcat gataaagaca atccaagatg tggcactaaa
     6961 aagctgagca gtcatttcaa ctggatcaat gatcgtcgtg tattttttgg ctagaagtgc
     7021 tgttcctatg agcaaaacaa ctaatagaac agataaaaca gcagttgact ttttcttgat
     7081 caatttaaat aattcttgtt ttacaagcaa tgtcattttt cttcttcccc ctttactgaa
     7141 tccagaattt ccaatagaga agattccaaa tcattcgtca gatgctggac gtctgtcaca
     7201 tggatatttt gtgcagacaa tagctggatc acggttccta ataattcttc gttttctttt
     7261 aaaagcttga ttttatcagg atcttccaaa gtatatccag cttctgtcaa gatcgttcga
     7321 gcagctagat catttgttgt agagataacg atgaaatctt gcgctgtttg gttcaattca
     7381 gccattgtac attgtttgat gatctttcct tgattgatca ggatcagctc atcgaccatt
     7441 ttttcaagct cgctgagtaa gtgactggag atcaagaaag aggtgccctg agtagccaag
     7501 tcggtaatga tttttcgcac atctcttgtt gcttgtggat ccaaaccgtt catcggctca
     7561 tctaaaataa cgatagacgg atggttcagt aaggcaagag caattcccaa tttttgtttc
     7621 attcccaacg agtaggattt cgcttttttg tgaatgtact tttccatctt gagctgattc
     7681 acgatagtgt cgatagcaga tacatcgttg gtttcagaga ataacttcag atgatcatag
     7741 cctgttaaga atggatagat acccgggtat tcgatcaatg ctcctacttg ttccaaccct
     7801 ttatgactag agggagaaat ctcattttca ccaatacaga ttgttccatc tgagtaatgg
     7861 atcagtccta aaatagcttt catgatcgtt gtttttccag cgccattagg acctactaat
     7921 ccaacgattt tgccagggga taagctaaaa ctgacatcgc tgagggcttg tttgtgacca
     7981 aagttcttgg ataattcttg gaccgaaaga cttttcaaaa atattcactc cttttctata
     8041 gatagtacaa gcatacacct gatgagtgga gaagtcgcta ggtcattgga cttatttctt
     8101 attttttcat aaaaaaattg aatattaaat aaaaaggctt attttttctg atcagtactt
     8161 attccaagtg tctagtggcc gctagtaatc ttgatgccaa tgatactgat gagtagcatc
     8221 cccacaaaga accaagtgga aagattcagc ttatcatgaa acaagaccac accaattatg
     8281 accgaaccaa ctgcgccaat ccctgtccag ataggataag agagactcat cggcaaactt
     8341 ttgattgcct ttgacaaaaa gtaaaaacta gcaatcatgc caatcactgt gtaaaaagaa
     8401 taattgattt tagaaaaacc atcactccat ttcatcatcg ttgaccagaa tacttccaaa
     8461 attcctgcta tgattaattc catccatgcc atacttacat tcctccttaa ctataaaaaa
     8521 atacacaaaa agaggaaaat catcccttca aagacctaat ggaatgatca tcctctttat
     8581 cctacccggt ggcagattgt atatcttttt ctgtagtata gcacacctat ccagatatcg
     8641 gcaatctcat actttttagt aaagtgtacc aagcactgac ggatatatcg ttagcggaaa
     8701 agaagatgaa tgctttgcaa tttcatagaa agaagttgtt atttattaat catttaaaaa
     8761 aaacagtaac tagaccttat ttctatatat tatcgggcaa taaattaaaa aaagctttac
     8821 cttacaataa gtaagataat ttttgtgat
//
LOCUS       DASJTC010000098         8665 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.100, whole genome shotgun sequence.
ACCESSION   DASJTC010000098 DASJTC010000000
VERSION     DASJTC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.100"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(263..2644)
                     /locus_tag="U4B19_002343"
     CDS             complement(263..2644)
                     /locus_tag="U4B19_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEN2078038.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2634..3281)
                     /gene="recU"
                     /locus_tag="U4B19_002344"
     CDS             complement(2634..3281)
                     /gene="recU"
                     /locus_tag="U4B19_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEN2078039.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3345..3884
                     /locus_tag="U4B19_002345"
     CDS             3345..3884
                     /locus_tag="U4B19_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEN2078040.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3974..4393
                     /gene="gpsB"
                     /locus_tag="U4B19_002346"
     CDS             3974..4393
                     /gene="gpsB"
                     /locus_tag="U4B19_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEN2078041.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4415..4782
                     /gene="rnpB"
                     /locus_tag="U4B19_002347"
     ncRNA           4415..4782
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U4B19_002347"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4991..6157
                     /locus_tag="U4B19_002348"
     CDS             4991..6157
                     /locus_tag="U4B19_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEN2078042.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6203..7699
                     /locus_tag="U4B19_002349"
     CDS             6203..7699
                     /locus_tag="U4B19_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEN2078043.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7780..8463
                     /locus_tag="U4B19_002350"
     CDS             7780..8463
                     /locus_tag="U4B19_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2078044.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2725 a         1571 c         1840 g         2529 t
ORIGIN      
        1 ttattaagaa gtggcactaa ataaaagact agtgtgtatt tagccgattt aattataaac
       61 aagtaaatgg ataaagtata tgtaatgata aaagaaaaat ggattttcta atttttaagc
      121 taaatgttag atcacacatg tatatgctgg atagtcttat aaaaaatcat gtaatacagc
      181 ttggtacatg atttttattt gtagctgttt tttacataga cacaaaaaag aaaccacgct
      241 tgtagacgtg gtttcttttt tactatctgt ttccagaagc tgaggtgctg ctggatggtg
      301 tatggtcatt tgcagcatcc gctccgctag ttgctggctc actggaagaa gcaggaggag
      361 cagatgaaga cggtgtcgag ctttcttcgg atgaagaaga aggaaccgtt gttgaagacg
      421 ctggctcgct tgtttctgct gtcgtgcttt cttttgagga ctcagcagtt gattcgcttt
      481 gactagtaga gctagagctt tgagtcgttg tctctgttgt tgagcttcca ggagtctgga
      541 catagctgga agatggaata gttgttgatg gtgtaattgc attcgatcta gctgtatatt
      601 gatctttgta gtacagttca ccgcccacac gaattaatcc gcttggcatc tcccagtctg
      661 tatttgtcac gttagctgaa acatactgca taagctcgcg ataaacatca gaggctacgt
      721 gtgacgattc tgaagtgaca ggcgtcattt ttttattgta acctgtccac acggaaatcg
      781 aataatttgg cgtatagccc gcaaatagga tatctggata caccccagaa gaaataccaa
      841 gttttgcata ctcgtcatct gtgtaattgg atgttccggt cttaccggct tgatataacc
      901 cagcgatttg cgcattcgta ccagttcctt cagtgatcgt atcttttaaa atgtctgtga
      961 tcatataagc tgtttctgga gacatggctg tttttccatc tggctcataa gtttcttctg
     1021 ttccgtcttg gaagacgatt ttattaacat attgaggttt gtaataagtc cctccattgg
     1081 cgaaagccgc ataggctgct gccattttta aggaagaagc tccgtatttt gtcccatctt
     1141 gttcttccgt attactagag atcgcattcg attgatggat cgtactgtat tcgatcccaa
     1201 gattttttaa gaaagaagcc actttatctg aaccgacttc attgaataat ttgactgctg
     1261 gaacattacg ggataagtaa agtgcttggc gtaaagtaat ggtgcccata tattgattat
     1321 cccagtttcc tacaggagtc gaagtccctt catagttata cggtgcgtca gtaatagttt
     1381 ttccagtaga atatttcaaa tattcaaaag ctggtccgta atctgtgaca ggtttcatcg
     1441 tagaaccaaa atcacgggaa gtattcactg ctaagttgtt gcctaaagtt acgtcttctg
     1501 cgatgtgacg tccgccaatc tgggctttta ctttcccggt attggtgtcg ataagggtgg
     1561 aggcaacttg catctcgtcg tctgggtaag agacatactg atccgtgttg acgatatcat
     1621 atagtttctt ttgggcgtcc agatccaaat tcgtataaat ctctaaaccg tctgtgtaga
     1681 cattcttatc tgttttttct tgtacttcat tgatgacttc tttgacataa ttatcgacga
     1741 tcttagtatt gtcatcggat tgagttagtt cttgcagacc atccgttacc ggtacattaa
     1801 cagcttgatc atattctgtt tgagagattt tttcgttttg aagcatcgta tacaaaacag
     1861 tatctcgtct tttcttcgcc tgatctggat aaacataagg atcataggcg gaaggggctt
     1921 gaggcatccc agcaagcaat gctgtttgcg gtaaagaaag ttctgataat ttcttaccaa
     1981 agtacatctc agaagctgtc tccatcccgt acaagccgtt tgacatatac actttattta
     2041 cgtagtaggt caagatctct tgtttcgatt ttttttgttc caatcttaca gccatccacg
     2101 cttcttgggc cttgcgtttc agagtctgat cctcggcact ggtggagaag aaggaaagtt
     2161 tgatcaattg ctgtgtcaaa gtacttccgc cttgcagtcc gccagaggta aagttagaga
     2221 gggcagaacc gatgatccga atagggtcaa ccccgatgtg tttataaaaa cggcgatctt
     2281 ctaccgacac gatcgcatct tctaatgttt tcggtaattc attcgcagaa attttttcac
     2341 gcttttctgc gcctaaatct tcgaatagtt cgccatcttg tgtatatagt ttggaagata
     2401 cggtagcgtc taatttttta tccgtcaatt cgggcgcatc ttttgcgtaa taccagaaaa
     2461 gtcctacacc tgctaaaaag aggatacaga aaaatgagag tattcctaaa aggatcttga
     2521 taaataatcc ttttttatgt gttccagagg actttccaga acctttgcca gaattcttct
     2581 tcacactctt tttaggagta ggctgtttct gtcgtctaga gcttctagtt tgttcatttg
     2641 ccattcttgc tttctccttc aatagataat gttgataaat agaagtcaac cgcgtccaaa
     2701 taaggaatcc gcggagaaat accagttttg atctctattc ctagttcttc gatcaaagat
     2761 aaaggtagtg atttgcgtcc tttttccttt tgggcgtccc agtaagcaat caaatgaaca
     2821 ctattcaaat aaaaacagcg cttcaaagac gaaaaccata acaagacaaa gcagacccca
     2881 ttttgttcga ggcatgcttt catatgttcg atttgatggg catggaagtt tttaaaggga
     2941 aaagatgttt tgttcttggt ttcttttgct tcaaagtcca agtaaaagcc tcgatagacg
     3001 ccattatagt ctgtagttga ggcttgggta aagtaggctt ccttgatcac agcagcactt
     3061 ctcttaggat agtctacttt cacgatttgg ataggggtag gctttttatg gataaccgcc
     3121 aacccgcggg aaagataata tttattactt tcattgattg cttcttcaaa gcgcatcccc
     3181 cgattgccga attcagtgat ctgatttctt ttttcttgaa ttttttcgac gggagaggca
     3241 ttcttataat aaggttggcc atttggatag cgcaatacca tctttaatca cactcctagg
     3301 ccacattata gcaaaaaagc ggatagaaag aaaaggaatc tgagatggat aagtcaaaag
     3361 taatctatgt gtctgggtat cgaagtttcg aactcggtgt ttttaaagaa aatgatccta
     3421 aaatccaagt tgttaaaaat gttttaaaaa aagaactcca tcagttagca gaagaagggc
     3481 tggaatgggt acttgtcagt gggaatttgg gcgtcgaact atgggctgtg caaatagtag
     3541 cggaattgaa agaagaatat ccggaactat ctcttggtat tttgtatcct ttttctgatt
     3601 ttggtaataa ctggaatgaa gggaaccaag aaaaaaaaca attagcagaa caattagcag
     3661 attatgtcga agctgttagc catcaaccct accaatcacc tagtcagcta aagaatcaca
     3721 ctcgttttat acttgagcat acagtgggct gtctgctgat ctatgatgaa gaattccctg
     3781 gaaaaacgaa gtattttttt gaagatgcac agaattacca agcagatcat ccctacgaaa
     3841 ttcggctgat cagtatggac gatcttcaaa attttagtga ataatcgata ttgtgtttga
     3901 tttatcaact tttttcctat acaatgaaaa aggtagaaat gttattacgt aaatgaaaac
     3961 tagaggtgta aatatggcga atttggttta tagtcctaaa gacattttac aacaagaatt
     4021 caaaacaaaa atgcgcggct atgatcccgt tgaagttgat gaattcttag acaatatcat
     4081 caaagattat gaaacttaca gcaaagaact ccttgcttta caagaagaaa acgatcgatt
     4141 gagtgcaaaa gtagctcaat tatctaaaac tcaaggagca gctcaaacac gcgtgcaaca
     4201 aacagaggca ccaaagagcg cagcagtgac caattttgat atcttaaaac gcttgtcaaa
     4261 tcttgaacgt gaagtatttg gtaaaaagct tgatcaacaa gcaagtgcag tcaaaccagc
     4321 acaacctaat ccaaataact acacaaatgc agacacaagc ttagatgata atgaaaaaac
     4381 acgtcaattt taatttttga ttaatgaatt tgcggttttc gggtaatcgc ggtctgactt
     4441 gatcaggctg aggaaagtcc atgctcgcac aggctgtgat gcctgtagtg ttcgtgctta
     4501 gcgaaaccat aagctaaggt actctttgga gtaacggcag gaaaacaagc taaggcttcg
     4561 gctatgctta agtatccttg aaagtgccac agtgacgaag cggctgggga aacccatccg
     4621 gtggaacgcg gtaaacccct cgagcgagca acccaaacaa tggtaggggc gctttttgtc
     4681 tggaagaaga acaggacaaa aaggcagtat aactgcagat agatggttac cgctttgttt
     4741 tttctccctg gaaaagcaaa gtacagaaca tggcttatag aaaaccgcaa acatcaggga
     4801 attaggtcgt gacaaaagta atgcgttata agaaacaaga aaaacatttg aaagctattt
     4861 ttcaatactt cagatgaatc gttcttatta caaaacaaac ttttgtatca ccggttattc
     4921 ggaatttgag taaaagtgtg gggtaggact gatgtcccac ctcctttttt tataaataag
     4981 gagattgtgt atggaaaaaa acaggaagtt caacctcgtc gcaactgcgg caagcgggct
     5041 agaagcactg gttggtaaag aattaagaga tcttggaatc gaatgccaag ttgagaacgg
     5101 acgtgcacgt tttgaaggaa caatggaaac gatcgctaca gctaatttat ggttacgtac
     5161 agcagatcga gtaaaaatcg tggttgggga attcgatgcg tatacgtttg atgaattgtt
     5221 tgaaaacgta aaagcattgc cttgggaaga ttttcttcca ctagatgcag cttttcctgt
     5281 agctggaaaa tcgatcaagt cgaaactata tagcacacct gattgccaag cgatcacaaa
     5341 aaaagcaatc gttgaacgtc tccgtaccta ttatcaccgt ccagcttctg taccactgac
     5401 ggaaactggc gcacattttc agttggaagt agctttgctg aaagaccatg tgatggtaac
     5461 tctggatacg acaggaccaa gtttattcaa acgtggttat cgcttagaaa aaggtggagc
     5521 accattgaaa gaaaacatgg cagcagcact tattatgttg acaaattggc gaaaagaccg
     5581 tccattttat gatcctgtat gtggttcagg tacgctttgt attgaagcag ccttaatagg
     5641 ccataatatc gcaccaggat tcaatcgtga gtttgcttgc gaatcttggg attggttcag
     5701 tcaagaaatc atggaaaatg ttcgatcagc agccgaagaa aaagcagatt atgatattga
     5761 attagatatt actggttcag atatcaatgg acggatgatt gaaattgcca aagccaatgc
     5821 agaagaaatc ggattaggct cttcgattac ctttaagcaa caagcagtca aagacttcaa
     5881 aacggataaa gaatacgggg tcatcgtggc taatccgcct tacggagaac gtctaggaga
     5941 agaagaaact gttcggaaat tatataaaga aatgggcgat gtattccgtc cattaaaaac
     6001 atggagtaaa tatatcttga ctagcgactt agcgtttgag gaattttacg gacaaaaagc
     6061 aaccaaaaaa agaaaattgt acaatggtgc attgcgtacg gatctattcc agtattgggg
     6121 aactcgtccg cctagaaaaa aagagaaaga cgaataatct tactaattga ttcattcaat
     6181 tggatgtttg gaggaatagg gaatgaaaga aaaagagttt ttacaagaag taaaagaaat
     6241 caatcttcta caacaagctt taggaatcct cgattgggat acacaaacag gtatgcctga
     6301 aaaagctagt gcataccgaa gtgaagtaga cagctatctt tacagcattt atttttcaaa
     6361 aaaaatcggt ccaaaaatca aagaagccat tcgctatttt tctgaacatc cggaagaatt
     6421 gtcggaagtt ggaactgccg tttttgccaa agtaaaagaa gagtatgatt tggaacatcg
     6481 cgtaccggaa gatcttatga gaaaatatac agctgccgtt tcttcagcac acggacaatg
     6541 gcaaaaagct agagaaacac aacaattttc tgattttcaa cacgcattga atcaaaatat
     6601 tgaattaaca aaacagttga taccttactg gagaaaagat gaaaagacga attatgatgt
     6661 tttgctgaat caatacgaac ccgggatgac tgtcgaaatc cttgataagg tatttgatca
     6721 gcttaaagaa ggaattcttt cgatccgccg tacgctccaa gaaaaaggga ctgaacctga
     6781 aacagacttt ttaaatcgga gaatgacgaa aaaacaacag aagagatttg tagtgaaagt
     6841 aatcgaacaa cttggttatg attttagtcg tggtcgttta gatgacactg tccacccatt
     6901 tatgactgga attaatccga atgatgcacg aatcacgaca cgatggaacg aaacagattt
     6961 caaaatggct gtttttggag tgatccacga ggctggtcat ggtatgtatg aacaagacat
     7021 cgatgataaa tatgcgtata caccggttta tgaaggtaca tcaatgggta tccatgaatc
     7081 acaatcgttg tttaacgaga tcattattgg aagtagtcgc agcttctgga aaaagcaata
     7141 tcctttcttt caagaatgtg cagaaggaac attcgatgat attccttttg acaaatttta
     7201 tcgttcattg aaccatacaa aatcaagttt ggttcgtatc gaggcagaca gtttgactta
     7261 ccctctccat atcatcattc gttatgaaat cgaaaaaatg attttcaatg aaggggtaga
     7321 tgttgctgaa ctacctgaaa tatggaatca aaaatatgaa gagtatctgg gaatacgacc
     7381 aacaaatgat gtggaaggca tcctacaaga cgttcattgg tcaggtgcaa gctttggtta
     7441 ttttccctct tatgcattag ggtacatgta tgcggcacaa ttacttcatt ctttgcagca
     7501 agaaatagat gtggaaaatg ttctagcgtc agacgattat tctccaatca aaaactggat
     7561 gtcagagcat atccatcaat atggtgcatc tagaaaacca aatcaattaa tcatggatgc
     7621 aacacatgaa ccgcttaatc cgcaatacct gatcgactat atgaaagacc tgtattttaa
     7681 tgtatatcag gtaaaataaa atacaattta caaagatagc tgaaattcat ctaatctata
     7741 gatgttttca gctatttact taagaaagaa gtggaacgaa tggataaatt acaagtgatc
     7801 gctgattata gtatggacca attagcgcat gatcaaacag gacatgggag cgatcatacc
     7861 aaaagagttg tcaagttagc tgaaaaaatt ttggagacgg aaccgcaggc agaccgcttt
     7921 gtgacgcttg cagcagctta cctacatgat acgattgatg ataaagtcgt taaaaatgag
     7981 aacgaggcaa agcaacagct tcgtgtattc cttcgctctt tgccaatagt agaggaacag
     8041 atctcgatga ttttttccat tatcgaaaat atgtcctttt ctaaaaatct ctctgaagcg
     8101 gccgaactat ctctggaagg taagatcgtt caagacgctg atcgtatcga agcattagga
     8161 gcaatcggca ttttgcgtac tgcttatttc ggcggtggac atgggcaccc gatttttgat
     8221 cctgaactgt atccgcaaac attcaaggac aaagaaaatt accgaaaagg cacgacagtg
     8281 atcaatcatt tttatgaaaa actgttcttg ctgtctgata aaatgaatac tgattacgga
     8341 tatgaagaag ccaaacgaag agaaaccttc atgcgtgaat tcttagaaga attttttctg
     8401 gaatggtcta ttgacgatga aaattttact ccagacaatt ggatcaaaaa taacaaaaaa
     8461 tgatggaggt tgtgacagaa gtggtcagct ccaagatatt tcggatcatt ttccgtaaaa
     8521 gttacttctg gaacactgtt taccaagagg tcgtgagcct gccgttcagc tccgagtcac
     8581 aaggaacaat ttcaaaaaat agcttctcgt gtttgatgaa tattgtaact tgtgattcgg
     8641 aattggcagg agaacaccgt ttatt
//
LOCUS       DASJTC010000099         8247 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.101, whole genome shotgun sequence.
ACCESSION   DASJTC010000099 DASJTC010000000
VERSION     DASJTC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.101"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            31..1185
                     /locus_tag="U4B19_002351"
     CDS             31..1185
                     /locus_tag="U4B19_002351"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEN2078045.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1207..1959)
                     /locus_tag="U4B19_002352"
     CDS             complement(1207..1959)
                     /locus_tag="U4B19_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEN2078046.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2323..4989)
                     /locus_tag="U4B19_002353"
     CDS             complement(2323..4989)
                     /locus_tag="U4B19_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEN2078047.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5330..6781
                     /gene="cls"
                     /locus_tag="U4B19_002354"
     CDS             5330..6781
                     /gene="cls"
                     /locus_tag="U4B19_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEN2078048.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7110..7925)
                     /gene="thiD"
                     /locus_tag="U4B19_002355"
     CDS             complement(7110..7925)
                     /gene="thiD"
                     /locus_tag="U4B19_002355"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEN2078049.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2411 a         1705 c         1470 g         2661 t
ORIGIN      
        1 gttttttatc ataagtaaag aggtgttgaa atgaaaatag ggattgatcg tctttccttt
       61 tttattccta atttatattt agatatgaca gagttggcag aaagccgtgg ggatgatcct
      121 gcaaaatacc atatagggat tggtcaagac caaatggcag tcaatcgtgc aaatgaagac
      181 atcattacac taggagcaaa tgctgccagc aaaatcgtaa cagaaaaaga ccgtgagcta
      241 atcgacatgg tcatcgtcgg aacagaatcc gggattgatc attcaaaagc gagtgcggta
      301 attatccacc atttactgaa aatccaatct tttgctcgtt cttttgaagt aaaagaggct
      361 tgctacggtg gcaccgcagc tttgcacatg gcgaaagaat atgtcaaaaa tcatccagaa
      421 cgaaaagtac tagtcatagc aagtgatatt gctcgttaca gcttggcaag cggtggtgaa
      481 gtgacgcaag gtgtcggtgc tgttgcgatg atgatcactc aaaacccgcg tattttatcg
      541 attgaagacg acagcgtatt tctgacagaa gatatctatg atttctggcg tccagattat
      601 agcgaatttc ctgttgttga tggtccttta tctaattcta cgtatatcga atcattccaa
      661 aaagtttgga atcgacataa agaattgtcg ggtcgaggac tcgaagatta tcaggcgatt
      721 gctttccata ttccgtatac taagatggga aaaaaggcat tgcaaagcgt attagaccaa
      781 acagacgaag aaaatcagga acgtcttatg gctcgctatg aagaaagcat ccgttacagc
      841 cgacgaatcg gtaatcttta cactggttca ttatacctgg ggctaacttc tctactggaa
      901 aattcgaaat cactacagcc aggagatcgc atcggtctat tcagctacgg ttctggtgca
      961 gtaagtgaat tttttacagg ttatctggaa gaaaactatc aagaatatct cttcgcacaa
     1021 tctcatcaag agatgctaga ttctcgaaca cggatcactg ttgacgaata tgaaacgatt
     1081 ttcagtgaga cgcttcctga acacggagaa tgtgcagaat atacttcaga tgtacctttt
     1141 tcgattacaa agatcgaaaa tgatattcgt tactacaaaa tataataaaa tcataaaaag
     1201 aacccttcac gagtcgtgaa aggttctttt tttatctgcg gaccaaagga gcagtctata
     1261 ggtcagctta gtcgtactaa ggatttctcg tttttcactg aatagtttgc gaatgtacct
     1321 ggtcacaaca ggatcttttg cgtacgcgtc atagtatagc ttcatcaatt cttcagtaat
     1381 tgattcctgt gtaaaataag taaaatcttt tttcttcact ttgaatcctt catcaattaa
     1441 gtatgtttca aattgatcca ttacaactgg cgatgtatta ggatcttctt ctatcattcc
     1501 tagttgcttt tcgataggat aaaaaacatc atcgattgat tccactactc tgattatcac
     1561 gcaccatttg cttgactttt tcatcaattt ttctaattga ataagatgta ccaatgctgg
     1621 atttgctgct gtaaaaacca tttcatatgt ctcatttgat tggataaatt cttctaacga
     1681 ctgtttgata aaggaaacat ttggcaactc atttttttct gcttcttttt ttgcattttc
     1741 tagcattcct gaagaaaaat cgatcaaatc atagcgatta atttcttttg ctaaggaaag
     1801 aaatctgcca gatcctccag caacgtctgc tgctgtttca gcaggcagga ctttctccgt
     1861 tttcagataa ttttttacat cttcagcaat agttacttgc gattcttgct ggatttcata
     1921 atattcttca gcaaattcat cccattcttt ttcattcatt tgatttcctc taattttttg
     1981 ttatatgttt tcacttttcg gaataaatca ttctcctctt ttatctattc tatctctttt
     2041 gatgaataat aaaatgattg gctttccgtc tatcataaat aaaaaaaggg actgtgacca
     2101 aagtcgtgtc aaagtccctc tttccgaata aacagtgtcc aatcagatga ccctcagtat
     2161 tagctcaaaa tgctgatttt acaacttctt ccttaaacgg tgttctcctg ccaactcttc
     2221 ggtcacaagt cacaatattc aacaaacatg agaagctgtt tgttgaaatt gcaccttgtg
     2281 actcagaatt aaacggcagg ctcacaacct cgttgattcg ttttatttat ttccttgtac
     2341 tcgtacaatg atcaatttag tgatttccat caaaacaatt gtcacaagtg cgattcccat
     2401 acatgtaaag acttctgtca atccaaatgc tgatgggata gagaagactt ctttcagtcc
     2461 tgggatcatt gttaagcagt aaagcgcgct acatacaact acagctagta agacacttct
     2521 gttagagaag aaccctgctt ggatagctgt ttgtgtgttt gaacgtgctg ggaatgtttg
     2581 caatgtccga caccaaatca atgttgaaaa tgacatggct acaccgaatt caggtgaatg
     2641 ctgcatgcca agccattgag caaagataac agcaatccca atcagcacac cacgatacgt
     2701 tacagaaatc aatgttttgt ccgtaaagat tcctgaattt ggatctcttg gcgggcggtt
     2761 catcgtagca ggttcagctt tttctgtacc taatgcaatt gctggcagtg aatcattgac
     2821 taaattgatg aacaacagtt gtaatgctgt aaatggattg gaccagtcag caactaaggc
     2881 aaagatgatc gcaatgatcg cacctaaatt tcctgcaaat agataagaaa tagctttctt
     2941 gatattatca aacacattac gtcctacttc tactgcgctg acaatagaag caaagttatc
     3001 atccgtcaat accatcgctg ctgcatcttt tgccacatct gtacccgttc ccatagcaat
     3061 tccgatgtct gcttgtttca atgctggtgc gtcatttaca ccatcacctg tcatagctga
     3121 gatatgacct ttattttgcc aagcacgaac gatacgaatt ttattctcag gtgaaacacg
     3181 tgcataaaca gtgacttttt ctaaaacttc gtttagttga tcttctgtca attcatctag
     3241 ttccgtcccg gtcaaagcca ggtttccttc ttcaaaaatc ccgatttgtt ttgcgatagc
     3301 aacagctgtt gttttgtggt caccagtaat catgattgtt ttgattcctg cacttcttgc
     3361 ttctgttacc gcctgagtca cttcagcacg tggaggatcg atcatagcaa gcaaaccaac
     3421 aaggatcaaa tcgtcttcat cttcaaatga taaatcaaaa ttatcaagtg gacggtaagc
     3481 aaaagctagt acacgtaatg cgcgtgttga aaaagcttca ttttgctctt ggaatttttt
     3541 catcaattca tccgtcatcg gaacgacttc accatttagc aatacttttt tactgcgttg
     3601 gaagacgata tctggtccgc ctttaacaaa caagtaattt ttgttatcaa ttgtgtgtcc
     3661 agtagacatt aattttcgat ctgagtcaaa aggaagttct gcttgtcttg ggtagttgtt
     3721 tcggatttcc tgataaggct ggtttaattt ttcactaaag tcgataaacg ctacttccgt
     3781 agggtcgccc aatttagtac cgtcttcact gatcgatgca tcattcgcta aaacactgat
     3841 ttcgatcaag cgacgttcgt cagctgtcca ttttgaaggg tcatttgtga aatccccagt
     3901 atcgccatta cctaaaaagt agtcaacaac tgtcattttg ttttgtgtca atgtaccagt
     3961 cttatctgta cagatgatac ttgtcgaacc aagtgtttca actgctggaa gcttccgaat
     4021 aatggcatga cgttttgcca ttttattcgt tcccaaagat aacacgattg ttacgataga
     4081 ctgtaatgct tctggaatag cagctacggc tacggctacg gcaaacatca ctgcgctgac
     4141 gatatctgca gtcatatctc ctgtaccttc tccaagataa atacgaaaca gttggatacc
     4201 taaaatcaat aaagacaatg ccaaaatagc caaacttagt tttttactga atttatccag
     4261 acctcgttgt aacggagtga cttgctcagt tgttgattcc aaaaggccag caacttgtcc
     4321 gatctccgta ttattcccag ttgaagtcac taagaattta ccgcgtccgt aagttacgat
     4381 cgttccactg aataccatat ttgtacgatc tccgattggt accgtacttg tgatatccgt
     4441 tacttctttt tctgcagaag tcgactctcc tgtcagcata ccttcatcta cttttaatga
     4501 tcccgcatct agtaaacgtc catcagcagg tacataatcg cctgcgtcta acaacacgat
     4561 atctcctacc acaagttcgt ttgccggaat cgttttttcc ttaccatcac gaatcactcg
     4621 agcatcgggc gctgaaaggt ttttcaacgc ttctaaagaa ccttctgctt tttttgtttg
     4681 gacaacactg attatagaat tcaacatcaa tactgcaaaa ataactaaga attcgatgta
     4741 gtcacccatg accatctgaa tcagtgcgac aatcagcaat acgataacca ttgcatcttt
     4801 gaaagtttct aagaacaatt tccaagttgg cttcttcggc ttttctttta gctggttcaa
     4861 gccatctctt tctaattttt ccttcgcttg cgtggtgctc aatccatcgg ttgagctctg
     4921 cgtttgctgc atcaacgagt cgatcgtttc tttatagacc gcttgttgtg cttgttcctt
     4981 gttttccaaa tttcattccc cattttttga tcttggccct agtcctgtct gaaaaaaaag
     5041 agacctaaga caaatacgca tttgtcttag gtctcaccat ttcatacaat accagaaaac
     5101 catttggtta acttactgtt gatattatcg caacaaacgt tgccggttac tcccctaagg
     5161 agatattcaa ctgtactaat atcctacctt attaacctac aaaaagcaac tactttagcc
     5221 cgatttacct acattttcat gaaaggagtg aaaagtatat tcaccttggt aggcttcttt
     5281 cttctatcat gctatactaa tgttattgta tgtaaggagg aacatttttg tggtatctag
     5341 tattataacc gccctttatc tattaaatgc acttattgct ttggtggcta ttttgatcaa
     5401 accccgagat gtagcagcca tttgggcatg gctcttagta ttgtttgccc ttcctggggt
     5461 gggctttgta ttatatttat ttttcggacg tggattaacg gataagaaaa aattctatct
     5521 ccgacaaagt gacttgaaag aattagaaaa ctttcagtct tttagaggag ataccattga
     5581 acattacgat cctgacatgg gcgataaaga caaacagcaa tttgttgact tcttctcttc
     5641 attaaatcgt atgccgctga caagaatgaa ttctgtcact cttctcacag acggacaaga
     5701 gaaattggat tcactgcttc aagatctaaa aaaagccaaa cattcgatcc atatcgaata
     5761 ttacgcattt gtgacagata atatcggcca gcaagtctta catgttttag aagaaaaagc
     5821 cgcagaaggc gtggaagttc gaatattata tgatgcattt ggctctcatg gcacaaaagc
     5881 aaaagatttc aatcgtctaa tcaaaaatgg tggacatgtc catacatttg ttacctcaca
     5941 aagggcatta cttcgtttcc gattgaatta ccatgatcac cgaaaaatcg ttgtgatcga
     6001 tggaaagatt agttataccg gtggtttcaa tattgccaat caatatgtaa atacaacaaa
     6061 aaaattcggc tattggcgcg atacgcatat acggattttc ggtgccgctt cttctttgct
     6121 ccagcttcgc ttcttaacag actggaacgt ctcggtacct gaagaaaaaa aggtcggcta
     6181 tcatttgaat tatttcttta aaaaagcaga tcgagatgaa tctaagcttg ctgatacatc
     6241 catccagctt gtttcaagcg gaccgaataa cgaaagggaa caaatcaagc tttcatttat
     6301 caaattgatt acttctgcta aaaaacgtgt ttggatacag acaccttacc ttgttcctga
     6361 tgaaagtgtc attgctgctt taaaaatcgc aactgcctct ggtgtagatg tgaaaatcat
     6421 gattcccaac aaaccggatc atccttttat ttatcgagca acacaatatt atgctcggca
     6481 gctgatcaag gaaaatgtac aaatccttgt ctatgagaac ggcttcctcc atgcaaaaac
     6541 attgataatg gatgatgaaa tctgcatggt aggttcagca aatcaagata ttcgtagcta
     6601 ccgattgaat tttgaaacaa gtgctgtcat ttacgatcct gagtttttag aagaacttgc
     6661 tactcagttc aaagaagatg agacacattg ttcatccatg acaactgaaa cagtcaagga
     6721 aatgtctaac tggctattat tcaagcaaca aatttctcga ttattttctc caatcctata
     6781 atttttttat tcaagtagag aaaaatatac taagattaaa gggactgtga caaaaatctt
     6841 gtcacagtcc cttcttccga acaaacggtg ttctcctgcc aactcctcgg tcacaagtca
     6901 caatattcaa caaacatgag aagctgtttt ttgaacttgt tccttgtgac tcggagctgg
     6961 acggcaggct cacgacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa
     7021 gtccaaccaa gttaaaaaca taagaagctg tttttacttg gctgttctta ttacttgatg
     7081 cagggcaact ttttcacaac ttctttcttt tactctaatt caccagctgc ccaatgattg
     7141 atggggccat atttgtgccc tacatttatt tcttcacgaa ttgcacggtt tacatagtct
     7201 tttgccaatc ggatcgcttg ttcaactgtt tttcctttcg ctaactcagc tgcaatacat
     7261 gcagatagcg aatcgccagt tccgttgatc cggtcggtag gataaaatgg tgcatttaac
     7321 caaaatgatt ttcctgattc gaacaatacg tagtcattga ctgtttcttg ctgcgcattt
     7381 ttagcatgat gtcctttgat cataacgttt tttgcaccca tatcttgcag ttcttttgct
     7441 gcttgcacaa aatcttcttc agctgctaat gtccgtccag tcagcttttc tgcttcataa
     7501 aaattaggtg tcagtacttc ggccaaaggg acaagctttt ctttcaagct ttgcaaagca
     7561 gattcttcta gtaaaagatt tccatgtttc gttacgatta cgggatccac gactaatggt
     7621 ccaaaatcat atttttggta attttttaca actgtctcaa tcaactttga atctgctagc
     7681 atacctgttt tagctgcacg tatttgaaag tccttcgcaa tcagttcaaa ttctttgtcg
     7741 ataaaatctg ttggcaatgt cacacttgct ccgattccgt aagaattacc tgcaacacat
     7801 gcggtcatga cagaaacacc gtaaacattt cttgtaaaga atgtatgcat atctgcttgc
     7861 atccctgcgc ttccatcgct atctgatcct gcgatcgtca ttgcttgtac atactgatct
     7921 tccatcaaat cgattcctcc atttttataa aaagattttt cataatactg ttactgtttc
     7981 tatcatatgt ttttcaaagt atccctcagt aaatatacca ctaccttttc ttggtaatca
     8041 aatttatttg aaaaaacatt tgcaataaat cagaaaatag tggtcgttta tttgtattcg
     8101 ctattctaat aataaataac cactatttga tttatatata tttcagtatc cttcttgttt
     8161 tcatataacc aaaggcgcta tgatttccat atgaaaaaaa taaagtcagg tgaaaaaaat
     8221 gaaacttgag gataaaaaag tcatttt
//
LOCUS       DASJTC010000100         8200 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.102, whole genome shotgun sequence.
ACCESSION   DASJTC010000100 DASJTC010000000
VERSION     DASJTC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.102"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1087
                     /locus_tag="U4B19_002356"
     CDS             <1..1087
                     /locus_tag="U4B19_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEN2078050.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="U4B19_002357"
     CDS             complement(1305..2447)
                     /locus_tag="U4B19_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2078051.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="U4B19_002358"
     CDS             complement(2452..2637)
                     /locus_tag="U4B19_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEN2078052.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="U4B19_002359"
     CDS             complement(2991..3236)
                     /locus_tag="U4B19_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2078053.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="U4B19_002360"
     CDS             complement(3230..3631)
                     /locus_tag="U4B19_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2078054.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="U4B19_002361"
     CDS             complement(3818..5626)
                     /gene="uidA"
                     /locus_tag="U4B19_002361"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEN2078055.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(5649..7415)
                     /locus_tag="U4B19_002362"
     CDS             complement(5649..7415)
                     /locus_tag="U4B19_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEN2078056.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(7428..>8200)
                     /locus_tag="U4B19_002363"
     CDS             complement(7428..>8200)
                     /locus_tag="U4B19_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEN2078057.1"
                     /translation="DSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQDWNNINLY
                     SETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQSEDLLKAL
                     KWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEEWERHELLS
                     LVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFFYPILVNKE
                     KESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
BASE COUNT         2627 a         1573 c         1106 g         2894 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gctaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caacatataa accactccta
     5641 tctaaatatt aaataaatgt atcctttaaa ccacaataag catctattgg acttactccc
     5701 ttaaattcag aaagacccat tagtttgtcc accaatattt ctaaagtttc ttgttttcca
     5761 tcataagtat taatataagt ctttacttga ggtacatcag ccaaatgaaa cggacactgt
     5821 acagatacaa aaattgttgg aatttcatga atgtaaaacg gtatatccgg agttccttta
     5881 cttgctggcc agacaattcg ttgaactgtg cctggattaa cattagctat attaatgatc
     5941 aagtcatatt ttttagttaa atttttaatt ggtctcttag cagcatatat tccagctata
     6001 tttttaattt tttcttcttg cgataatcct ttcaattctt cttcaggtga aatgtatttt
     6061 tcaacttcaa atccttttgc tgacaaaatc tgtttcaata aatcaatggc ttgaggtcta
     6121 tttccagcaa ctacttcacc aaacccacct tttaccccat gtacatctac tagtaaaatc
     6181 cttttatatt tttctggtac aataggccaa atatcttgtt tatctttaac taatgtaatt
     6241 gcttggtcag ccacctcttt aaaaattgat ttattttcaa ttaaaccaat tttttccaga
     6301 gcttcttgct taggtaataa aatttgttct tttgcttttt tgtgtaatcc caatttagct
     6361 tttaacccta aaattctttt taaggcatct aataaccgat catcagtgat aattccttta
     6421 cgatagcctt ccatcatata attaaagtct tcatctggat cattaaaaaa tagaaataga
     6481 tcacatccag ctgcaataga agtgggcaat aattctgatc gtttcattgc cccagtcatt
     6541 cctaacatat gactagcatc agtaactact aatccattaa atccgagttt tcctctaagt
     6601 aagtcagtga ttaattcttt tgataaagtt gcaggaagta aatcttcatc tttcagatca
     6661 ggattaaaat gttttgaata agctggcata tggatatgac ctgccataat tgaaggtaat
     6721 ccagcttcta ttaagcctga gtatactttt ccaaaagaat tatcccattc ttccgtacct
     6781 aaactattaa tactaaaaga tagatgctga tctcgttcgt caataccgtc accagggaaa
     6841 tgtttagctg caggcaaaat tccactctcc tgaatacctt tcatataagc taaactaagt
     6901 tctaaaactt tatcagggtt ttgtccaaaa gatcgactag aaatgatagg atttctccaa
     6961 tttctattaa tatctacaat aggagcaaaa ctccagttac atccaatagc cgatgcttca
     7021 atcccagaaa ctctccctaa ttcataagca tatttcgcat catttgttgc accaattttt
     7081 acttctacac caacttcagt accatctgta caagctccgt taccacctgc ttccgtatta
     7141 gcagcaatta atagaggaat tttactttct gtttgtaaga tatagttttg atcatatacc
     7201 tcatcagcag ttcctggatt atatcgtaca gctccaatat ggtactgatt gaccatatta
     7261 ctcaagtaat ttttatcacg actagatccc atattaacaa acaattgacc tattttttct
     7321 tccaaagtca tagaatcaat cgtctcttca atccaagaaa tagcatcttc atctaaatta
     7381 tatggttttt ttcgtaaatc tacttttata gtcattttct ttctcctcta cttattagta
     7441 agatttttcc aaaaagtatc tatatatttt tgttcaatat ctacataatc tcccgataaa
     7501 catttaaata aaacatcagt tcttaagttt tcccatgatt ctttttcttt attgaccaaa
     7561 ataggataaa agaaaacttt atttttattt gcagcttcta gatcaccagg agaatcacca
     7621 atcattaata tcttatccaa atcacaacca ttttcaatta cacttcgaat aatatcttct
     7681 ttttttcctt tatcttgaca acacagttca tcaactaatg ataataattc atgtctttcc
     7741 cattcttctt gaacagcttc cttattagct gagcttacaa taattatctt cccatatttt
     7801 ttcagagctt ttatagtatc tctagcactt aaaaagggtt tatctaatcc ttttgcttga
     7861 ctaatctgtt gattgacttc ttccgaccat tttaaagctt ttaataaatc ctctgactga
     7921 gaatattcta tttccctctt caaagatgga ttagacaatt cttttgttag agatacccag
     7981 tgctttaatc gattaatcga tgttacatca tttttttcta atgttttaac taaacctaaa
     8041 aatctgttta ttccacgagt ctctgaatat aaattaatat tattccaatc ctgtaggaat
     8101 ttttctctat gatgaacatc aaaaatctct gcagcaattg gtccgaaaaa gagattgtgt
     8161 ttataagtca tcgtatccat aacacagcca tctgaatcaa
//
LOCUS       DASJTC010000101         8166 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.103, whole genome shotgun sequence.
ACCESSION   DASJTC010000101 DASJTC010000000
VERSION     DASJTC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.103"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            476..1339
                     /locus_tag="U4B19_002364"
     CDS             476..1339
                     /locus_tag="U4B19_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEN2078058.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="U4B19_002365"
     CDS             complement(1468..3090)
                     /locus_tag="U4B19_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEN2078059.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="U4B19_002366"
     CDS             3339..3860
                     /locus_tag="U4B19_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN2078060.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="U4B19_002367"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="U4B19_002367"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEN2078061.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="U4B19_002368"
     CDS             5941..6759
                     /locus_tag="U4B19_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEN2078062.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="U4B19_002369"
     CDS             complement(6862..7059)
                     /locus_tag="U4B19_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078063.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="U4B19_002370"
     CDS             7228..7806
                     /locus_tag="U4B19_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN2078064.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="U4B19_002371"
     CDS             7901..8038
                     /locus_tag="U4B19_002371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078065.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2444 a         1475 c         1611 g         2636 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagata
//
LOCUS       DASJTC010000102         8143 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.104, whole genome shotgun sequence.
ACCESSION   DASJTC010000102 DASJTC010000000
VERSION     DASJTC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.104"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            382..570
                     /locus_tag="U4B19_002372"
     CDS             382..570
                     /locus_tag="U4B19_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEN2078066.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            648..1661
                     /locus_tag="U4B19_002373"
     CDS             648..1661
                     /locus_tag="U4B19_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2078067.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1728..2762)
                     /locus_tag="U4B19_002374"
     CDS             complement(1728..2762)
                     /locus_tag="U4B19_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2078068.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2877..5102
                     /locus_tag="U4B19_002375"
     CDS             2877..5102
                     /locus_tag="U4B19_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN2078069.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5120..7003
                     /locus_tag="U4B19_002376"
     CDS             5120..7003
                     /locus_tag="U4B19_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEN2078070.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6984..7958
                     /locus_tag="U4B19_002377"
     CDS             6984..7958
                     /locus_tag="U4B19_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEN2078071.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2638 a         1480 c         1712 g         2313 t
ORIGIN      
        1 gttagtctac attattttgg cgatttgcca ataaggactg ttgagttgtg caaaatcaaa
       61 tctgggtcag aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact
      121 gattgatcaa aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg
      181 tttgacgcgg aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga
      241 cccattattt tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg
      301 ctgggagttt tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg
      361 actgtaacga aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa
      421 gcgacacgat atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca
      481 acagaagaag aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca
      541 ttgcagaaga agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac
      601 actatggtat aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa
      661 ttcttaaatt tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc
      721 ttgccttcca gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata
      781 ctgttcaaat cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa
      841 cagatttgac gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa
      901 acagcaaggg gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag
      961 ataaatcggg tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg
     1021 ttgcgatgaa ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga
     1081 atgaactgat caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac
     1141 aattattttt tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac
     1201 aaacgaatga tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc
     1261 tgaaaggaac catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt
     1321 cggatgatcg atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa
     1381 aagattggaa aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg
     1441 aatttccgcc aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag
     1501 cattagaaaa tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata
     1561 caaaaaaaga gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt
     1621 gttaccagca gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt
     1681 ttcgttttga gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt
     1741 gttcttggga taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt
     1801 tcgtcccgta taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat
     1861 ttcgcattga tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta
     1921 tcaaagcttg ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga
     1981 tagacaccaa ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca
     2041 ttttctaata gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga
     2101 taagacatat atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc
     2161 ttttcattta tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg
     2221 atataggcta ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc
     2281 cgttcaagat ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg
     2341 actatttgca tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca
     2401 acgatcaccg cacctaactg atctagatct tcccattgtt taggattatc agatagatta
     2461 tagattcgat tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa
     2521 tgtttgtaat agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc
     2581 gaaggcattc tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct
     2641 gcctctacta tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac
     2701 acacgggaaa ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc
     2761 atttttacac actcctcaaa aaaataaaaa atttttattg acaaaattgt ttagtaaaca
     2821 gtatacagga attataaaaa aaagtaaacg gtttccgttt tgttgggagt gaaatgatgg
     2881 atattacgca tagaaaaaca gataaaaatt tcatcttatt tgatgaaaag agccgagtct
     2941 tccatttacg aaatacattt ctttcttatc tgataaagat agaagaaagc aatgtattgg
     3001 cgcatattta ttttggcaaa ccagtcaagc agtacaaaga caataaaaat tatccccgaa
     3061 gagatcgtgg attttcagga aatgttccct taaatcctga ccggtctctg tcgaaagata
     3121 cactaccgca agaatactct tctcatggca gcatggactt tcgaacgccc gcatcgatca
     3181 ttcagcgcaa gaatggatca gacttactgg atctgagata tgacagccat tatattacag
     3241 atgggaagcc agagatcgaa ggcttgcctc aaacttatgt gatggacaag tcagaggcac
     3301 aaacgttggt gatttcactt aaggatagag aaacagcgat ttatttcgat ttgttctata
     3361 ccatatttac tgatagagca gtgatcacac gatctgtaaa gataagaaat gaaaccggag
     3421 aaacaatcaa actagaaaaa gcggctagtt tccagctaga ctttgcccat actaggcgat
     3481 ttgatgaagt cattgctttg ccaggagcac atgtcaatga aagacagatt tcacggcaat
     3541 ctgttttatc tggaacaaaa gtatttgaaa gccgtcgagg aacgagcagt caccatatga
     3601 ataactttat tgctttagtt catcatcata cgacagaaaa tactggagaa gcgatcggac
     3661 ttcaatttgt ttattccggc aaccattctt tcgaacttga aaaagatcag atcaatcaat
     3721 tgagagtcgt cggtgggatc aacagccatc gtttttcgtg ggaattgaat gcaggccagt
     3781 cttttcaaac acctgaaatg atcctttctt actcaagtca gggattgaac aaaatgagtc
     3841 agatccacca tgagttgcta cgtgaaagaa tcgcaagagg tcgtcatcaa tttgctgaac
     3901 ggccgattct ggtcaataac tgggaagcca cttactttga cttcaatagt gaaaaaatca
     3961 aggcgatcat tgatgaagca aaggagctag gcatcgaaat gttcgtatta gatgatggct
     4021 ggtttggaaa aagagatgcg gacaattctt cattaggaga ctggttcgag tatgaaggca
     4081 agttgacaaa tggattacgt gaaatagcag attatgctca tagcaaagga ttgaaatttg
     4141 gtttatggtt tgaacctgaa atgatctcgg ttgattctga actgtatcga acacatcctg
     4201 attttcttat gcaggagcca ggtagaatgc cttctgcttc cagatcacaa catgtactgg
     4261 attttacaag gttagatgtt cggcagacga ttgagaaaca aatgagaaag attttagata
     4321 cgattcctct tgactacatc aaatgggata tgaaccgttc gctttcagat gtctattcga
     4381 ttacccttga cccacagaga caaggagaag tagcgcatcg ttatatgctc gggctttatg
     4441 aactacttga acatttgaca actgattatc cggaaatctt atgggaaggc tgttctggtg
     4501 gaggtggacg gtttgatgcc gggtttattt attatatgcc gcagtcatgg acaagtgata
     4561 acacggatgc agtggagcgt atgaaaatcc aatatggaac aagccttgct tatccgattt
     4621 cgtccatcac agctcatgta tcagctgtac caaatcatca gaccgacaga tctacttcgt
     4681 tgaaaacaag aggagaaaca gctatgagcg ctgtttttgg ttatgaactg gatttgacaa
     4741 agctgtctcc tgaagaaaaa aagcaagtaa aagaccaaat tatatcctat caaacgattc
     4801 gtccggtgat ccagtatggc cattattatc gtttggcgag tccttttgaa gaaaatattg
     4861 ccgcatggat gtttgtgtct cctaaacaag atgaagctat tgtgttttta gggaggatac
     4921 tagcttcagc tcaaccagcg ttccatgaag tatatctgat ggggttagat gatgaggcac
     4981 tttatcagga acagacctcg aagcggatat tttcgggggc cgaattgatg acagttggac
     5041 tttacttccc cgattttcaa ggtgatttcc aaacagaact gcttcatttc aaaaagttat
     5101 gagagagaag gaaaaaagta tgaaagcaaa tataaaatgg ttagatgatc cacaagtttt
     5161 tagagtgaac caatcaaatg cacatagcga tcatgtttgt tatgcaacaa gggaagaagt
     5221 agaaaccaca tcctccctta aacagtcttt aaatggatca tggcgttttt gttattcgcc
     5281 aaatccttcg gtacgaccaa aaacatttta taaaaaagat tttgatgcct gttcttttga
     5341 tgaaatcact gttcctagtc atattgaatt agcaaattat gacaagatcc attacatcaa
     5401 tacgatgtat ccgtgggagg gacatgtatt tcgccgtccc cctttcacga caagccctga
     5461 gaaagaggcg gaagggatct tttcagctgc taaagacaat ccggttggtt cgtatttgac
     5521 gacttttcga ttagaagaag gtctaaaggg aaaacaaatc tcgattcatt ttgctggtgt
     5581 agaacaagca atgtttattt ggttgaatgg tcattttatc gggtatgccg aagattcgtt
     5641 cactccgtct gaatttgatt taacacctta tttggaagaa gaaaatattc tagctgtgga
     5701 ggtttacaaa agaagtacag ctgcattctt ggaagatcag gactttttcc gattttttgg
     5761 gatcttcaga gatgtcttct tagttgctcg aggacaaaac catttagaag atctttggat
     5821 caaacctcgc ttagatgaca aatgtcaaaa tggcaaactt gaagtagatt tgtcttttga
     5881 gcaagtagcc aaaaacagtc agctgaatgt agcgatacta gatcctgatg ggaaggaaat
     5941 ctatcaacag caatatccgt taaaagagca aatgacgatt cgcatcgatg gatttgaaaa
     6001 tgtggcactg tgggggcatt ttcagcccaa tttatataag cttgaattgg agacgataga
     6061 cgaagaaaaa agagtaacgg agtatattga ctattccttc ggttttcgta aaatcgaaat
     6121 caaagataaa atcatctact tcaatggaaa acggatgatt atcaacgggg taaatcgaca
     6181 tgagtggcat ccgaaaaaag gacgcgccat cactatagaa gatatggaaa aagatttggc
     6241 tatcataaaa aatgcaaaga tcaacggcgt acggacatct cattacccca accagatcac
     6301 ttggtattat ttatgcgacc aagcaggaat ccatgtgatg gcggaaacga acctcgaatc
     6361 ccatggctca tggcaaaaaa tgggcgcatt tgagccttct tacaacgtac caggtgcagt
     6421 acctcagtgg caggcggctg ttcttgatcg agcaaaaacg aatttcgaaa cattaaaaaa
     6481 ccatccaagt attctttttt ggtctcttgg aaatgaatct tatgcggaag aaaacattcg
     6541 acaaatgaac gattacttca aaaaagtaga ccccgatcga cttgtccatt atgaagggaa
     6601 ttttccaaat ccagcattcg aagattcgat ttcagatgta aaaagctgca tgtatgcgcc
     6661 accagaagag atcattgact accttgaaaa taaagcggaa aaaccgttca tcttatgtga
     6721 atatatgcat gacatgggga attcgttagg tggaatggat tcttacatga cgttgcttga
     6781 tcgttatgaa atgtaccaag gaggctatat atgggatttt aaagatcagg cgatccaagt
     6841 gactgatcca atcacaggca gagaagtgat gcgctatggc ggtgactttg acgataaacc
     6901 aagtgactat gaattctcag ggaatgggat cgtttttgca gatggacaag aaaaacccgc
     6961 catgcaggag gtaagatatt attatgaaaa atacagtaaa taaaagtcat atggatacgg
     7021 aaaaagttgc aatcgtcttc ggcgactgta cattaggtgt caaatcgggg aatacgcatt
     7081 atattttttc ttatacaaga ggcggactgg aatcgctcaa taaaaacgga aaagaatggc
     7141 tataccggga aacacttcca gttttttggc gagcaacgac cgataatgac agaggtagcg
     7201 gatttggatt taaatccggt atgtggttag gagccggatt gtatccaaaa gtcaaagaaa
     7261 tagaagtact ggtggacaat aaaaagatcg atttgccgat tgctccagta aataatcaat
     7321 attcaagtaa tgaatacgct gaaacggttg aaatcatttt tacactggaa atcaacacag
     7381 taccaaaaac ccaagtgaca atttcctacc aaatcgctgg agagggtaaa atgcttgtca
     7441 atatgcatta ttatggacaa aaaggattac cggaattacc cgcattgggt ttaaggttca
     7501 ttatgccaac gaaagcaaaa ttttttgagt atgaaggcct atcaggggaa acatatccgg
     7561 atcggatggc tggtggtgtc gaaggtattt ataaagtggc aggactcccc gtttccccat
     7621 accttgttcc ccaagaatgc ggcatgcata tggaaacgaa atgggtagaa ctaacaagaa
     7681 atcaaacgtt ggataatacg gataaagatc aagaagattt ttcgttgcga tttgaacagc
     7741 aggatacgcc atttgctttt tccgctttac cattcaaagc agaagaactg gaacaggcaa
     7801 cacatataga agaactaccg ccagaaagac gaacggttct tacgattttt gcaaaagtaa
     7861 gaggagtcgg gggtatcaat agctggggag cggatgttga gtcaccttac cgaatcagca
     7921 gcgaagagga ccatgaaatc ggctttatta ttcagtagca atctttagat tattatttga
     7981 aaaacggagt ttacaagaca aagctgcttt attctatatg tcatgaacaa gagagtaaaa
     8041 aagttctcag atacttttgt tcatgactat tttcttcaat aaaaccaatt gttatttttc
     8101 tgaaaaacaa agttaactat cagaaaatta aaaaaagttt cat
//
LOCUS       DASJTC010000103         7812 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.106, whole genome shotgun sequence.
ACCESSION   DASJTC010000103 DASJTC010000000
VERSION     DASJTC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.106"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(32..1384)
                     /gene="celB"
                     /locus_tag="U4B19_002378"
     CDS             complement(32..1384)
                     /gene="celB"
                     /locus_tag="U4B19_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEN2078072.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1384..1902)
                     /locus_tag="U4B19_002379"
     CDS             complement(1384..1902)
                     /locus_tag="U4B19_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078073.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1918..2244)
                     /locus_tag="U4B19_002380"
     CDS             complement(1918..2244)
                     /locus_tag="U4B19_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEN2078074.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2257..4236)
                     /locus_tag="U4B19_002381"
     CDS             complement(2257..4236)
                     /locus_tag="U4B19_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEN2078075.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4257..4571)
                     /locus_tag="U4B19_002382"
     CDS             complement(4257..4571)
                     /locus_tag="U4B19_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEN2078076.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4739..5032
                     /locus_tag="U4B19_002383"
     CDS             4739..5032
                     /locus_tag="U4B19_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEN2078077.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5110..6222)
                     /locus_tag="U4B19_002384"
     CDS             complement(5110..6222)
                     /locus_tag="U4B19_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2078078.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6609..6788
                     /locus_tag="U4B19_002385"
     CDS             6609..6788
                     /locus_tag="U4B19_002385"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078079.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            6982..7152
                     /locus_tag="U4B19_002386"
     CDS             6982..7152
                     /locus_tag="U4B19_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2078080.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(7576..>7812)
                     /locus_tag="U4B19_002387"
     CDS             complement(7576..>7812)
                     /locus_tag="U4B19_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2078081.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1554 c         1262 g         2501 t
ORIGIN      
        1 ttttttgtca cagttccttt tttcttagaa atcattttaa cttttcacta gctaattctg
       61 ctgccttttc tctttcttca tttaataatt tattatcata gaatttgata aatgggaacc
      121 agataatgaa tgcgatgatt gcacaaatga ctgctaacac agcagctttc caatcaccac
      181 cactaccaac aaatgctccg atcccgactg gtgaaggcca cggcatctgt gcaagcatcg
      241 gtttgacgat ttccagccga atggcaaaat aagcgagtgt agcagaagcc atcggagcta
      301 agaaaaacgg aaccgctaaa tagggattgt agacgattgg catcccgaaa atgattggtt
      361 cattgatatt gaataatcct ggtacaagtg aggcttttcc taacactttc agctgatcag
      421 atttcgcgag gaatgcaata aagatgatca atcctaaagt cgcacctgat ccaccaacgg
      481 ttacataaga attgttgaat tctcctgcca acggataatt tgcgccttgt gcgttagctg
      541 ccatattagc taatacgatc ggggtcaaga aagaagtgat gatattcgct ccatggatcc
      601 ctacgatcca caatgcatgg atcaagaaat aaatcaccat gacaccaagc catgtattcg
      661 tcaactgcgt aacaaaccca aatgggatag caatcatttt gaagatatcc gttcctagtg
      721 caactaataa tccattgatg atcatcacca caaacgcaat gacaaatgtt gggatcagag
      781 cagtgaaaga acgagaaact cctggcggaa caacatctgg catttttatg acccaatttc
      841 tcttgacaca catacaatac aattcgaccg caataatcga cataataata gctgtaaaga
      901 ttcctgaagt tcctaaacgt tcgacaaaag agcccatcct aataccattt actacagttt
      961 ctgtctcatt gatagaagca atcaagctga ttttaccatc agtcatgatc aattcaggga
     1021 tacacataaa gaatgcaaaa acagatagta atgcccccgt gagcggatta acatttaatt
     1081 tttgttcatc tcgttcaatt gacgttaatt cataagcaaa cacaatggca aagtaaatag
     1141 ataaaatccc catcgtcatt gtattgacga tcatgtacaa gtcactaact ttgaaaaaag
     1201 ttgcattaaa aaatccttct aaaaatggga acggtaatgg caaaatattt aaaaccaaaa
     1261 acatcgagcc tacaatcgtg aaaggaatag aagccatccc cgctgccatg acagcccgaa
     1321 cgattttaag ttgcgcaatt ttccccatag gtcccatcag gtatttctgc aaaaaatcaa
     1381 acattagtta ttctcctttc ctaaatataa cgctttttca tattgtttaa tacggatata
     1441 ctgttggtct tggttgagtg aaattttctt taaacgatac tgtataaatg cactggcaaa
     1501 aataccgctt acaacaagaa tgaaaacaaa taactgtcct ccattgttat ttgccataaa
     1561 aggatacaag aatgtaaatc ctgaggtaaa caacactgga atcactaata aattaaagac
     1621 actctggaac aaaaaatacc actttgtcca tggaacgttc gtttggtgtt ttcgataaag
     1681 ctttacttgt tctcctactg caggcaaaat agctaaaatc agaatcatcg gtaaactact
     1741 tcctaaactt ttagctgata gaagcagaat catccattgc atgttcatga aaaagaaaaa
     1801 ggcagtcagg taacgtacga ttagataacg attgaaatac atgtttttca aactaagttg
     1861 ctttttttct aattcgattt tcctttttac tgtatcctgc acaatgatcc ttcctttcta
     1921 tttatttttt tgatacaaat aattcatttc ctgtgcaact tccaataatg tcatcgtcgt
     1981 catcagatga tcttgtgcat ggaccatgat aatttccatt tctatttttg tcccgctggc
     2041 atattctttc agaagttgag tttgtgactg atgcgcatga agaatctctt catttgcttg
     2101 tcgcaacttc tcttctactt cttcaaacat ctctttacgc atcgagcgaa atgcttcatg
     2161 tatcagtgtc cgtgcatttc cgctatggaa aatgatatca aaagaagtca cttgtagtgt
     2221 ttcactgttt attttttcag ccatctgttt tcctccttat tcgatcagtt tgataaattt
     2281 actgctgaaa ttctcaaaaa taggttcagc taagatttcc tgctgtactt ttagctggtc
     2341 aactaaccta acgattgctt tagtcacgac cgtaattcct tcattttcga cataagaagg
     2401 cgaaactaaa aaaacaaaat gaatttcttg atattgatcc cactgcatcc cagaaggaat
     2461 caatgcaacg gctacctttg tttcgattcc aactggaaca gcgggatgtg ggactgccac
     2521 tgtatcactg aatatgattt gtcccatatt ttcacgttgc tggatctgct ttcgcatttc
     2581 tcttatatat ccttcttctt catttatgct cagcttttgc agcaaatcat gaagaatttc
     2641 ttcctttttc ggttcgtacg tataagtctt gaaaaatttt tcagacaact gttcggtgta
     2701 aatctgcttt ttttgctgta gagaaaatgc tggtatcggt ctatctgagg aatgataggg
     2761 tatagattct ccaactacct ttcgtatttt acgaacatca tcttcattca aaaaaatact
     2821 gacatgtaaa actggaattt tgaaaaacat tgtcgaaaga tcaatagaag aaataatcag
     2881 gtcgattcct tttaaagttg tttcattgat ctcataatag ccttttacac ttaccactgt
     2941 aatattttta ccgaattcac tgactacgcg attctttaat agttgggcac tgccgtaacc
     3001 cgttgcacag ataatcaatg catgtagttt atgcgcagct ctttcttttt ccaatgccgc
     3061 catgagatgc aaagccaaat aggcccattc atcttcattg atggtatagt ttttcaaagc
     3121 cggcatgttg ctgaaatgcc tttttactgc gtcaaagact ttgggatttt ctttctttat
     3181 ctcctcagtc aatgggttct caatgcgaat acctcggctc aatcgaatca tcatcggtga
     3241 caaatgatcg agtagtccat tgaaaagctg gagatcctct actaatgaaa aatgaaattc
     3301 ctgagacaat cttttcagaa ctttcgctaa ttcctttgat agttcctgat cattttgatt
     3361 ttctttgtga ttcgacttag ccatgagatg taatgtcaaa taagcaattt cttcttcggg
     3421 aaacgataca cctgcgatta cctcaatacg ttgaacgatc cgctgcgcca cttgatactc
     3481 tactcgctta gaaacttctt gttcgatgcc taattccttg atttcaaacc cttctcggag
     3541 ccgtttgatc cccaaagcca agtgtaaagt cagattctgt atgataaaat ccgaaacttt
     3601 taacttagct tctcttgttt catcgagaat gataatgact agctcttcaa aactgatgtc
     3661 tgggaaaaaa cggctttgcc ctaaatattc tttcaatgaa tttccataat ggtttccaaa
     3721 aaaagtatcc atgataaaat gccgtcgatt tctttcttcc ccttcgatcc atgtcccttg
     3781 accatgctta gaaacgattt ttagtgaata tggaatcaat cgctccttga tttcttgcat
     3841 gtcctttgtc aaactggaac gactgatata aagctcttct gccaaatcat ccataaaaag
     3901 aatggcatct tccaaaagca attggttcaa tatatacttt tgtcgatctt cggactcata
     3961 aaatccttgc ggttctctag catatccagg tactatttct tgccgactca aaaacaagtc
     4021 aaatgtcagt ctttgtaaaa tatgcagttg gtagccataa cctggtttag caatgatctt
     4081 acctccgttt ttttctacta actctttgat ccgctgcaag taattacgga ttgtccgatc
     4141 agataataac aattcagaag ctaaacgttg actggtgaca tactccttct tgtgatcaat
     4201 caaatatctc aacagcaatt tttccttctg tttcattatc atccactcct attccatcat
     4261 atgttctctt cgatcacctc tgctaacttc tggatgcctg tagggattgg gacatacgct
     4321 tggaaaggga tactaacaac aggctttcca acagattctc ctaatttttt gaatttatcc
     4381 aaaaacatcg tcgtttgcgg gctgatcaga aataaatcaa aatcactatt cttaatcata
     4441 ttatcgcctt ctgtagctga aactgcatct acgagtacct cttctctttt ttgcttaaag
     4501 aaatccgttg tctttgaggc catcatcgag gatgacattc ctgccgcaca aataatcaat
     4561 gcttttttca taaatcaatc actcctttta tttttataaa ccaagcatat cttgtaagcg
     4621 ttttctattc aaaatatata tttccgtagc attgcggaaa aaatgtgtat catatcttga
     4681 ataataattt ctggcaccgt gatatactag tgatagaatt ttttcaggag gggatttagt
     4741 gagtatcaca gtgaatattt tgtatacaag tacaaatgga caagccagaa aatttgcaga
     4801 agaaatggtc gaaagaaaca ttgtcaaagc gattcgtgca gaagaaggaa acgagaaata
     4861 tgactattac tttccactag aagatccaga atctctttta ctgatcgacc gttggaaaga
     4921 tgaagaagca atcgaaaagc atcacaaatc cgaaatgatg gctcaaataa acgaactccg
     4981 taaaaagtat aaattaaaaa tgaaagtcga acgtttcact gatttaccgt aaaaagatac
     5041 acatggaatg atagcaattt atcatccatg tgtatcttca ctttttcctt attttttatt
     5101 cgatctcatc taatgattca aaccgtgttt ttctcttatt tttcggataa gtatttcagt
     5161 caccccaata tacatgattc ctaaacatac cccaaggata ttctcaaaaa tgcggatcgt
     5221 tactgctcct ggaaccccga ataaactggc agtgattgcc aaagcaccaa aagaattaaa
     5281 aactgtttta tatctatagg tagaacaaac acctaaagct agaccaccta atataccgac
     5341 ccagttaaaa ggaataaatt ggctgatacc aataaataaa acacatccaa tcaatgagcc
     5401 aatgattcgg tctactgctc gttctttgaa accaatcgac atcaatccat agctagatac
     5461 aatagaagaa aatgcaaacc ctgcccacat caatcgctga aaagggacat actctcctgc
     5521 aacgatcaaa agactaatgc caaatgcata gatgagcatc caaatttttt cctgcgaata
     5581 catgcctttc aaaaaaatct ctttaaacaa gcttttgtct ttgttctttt ctcgatgttt
     5641 tctgtaaagg agaactgaaa accaacaaaa aaacaaaaat aataagctac ttgtttgtgt
     5701 aaagaaattc ttgttcaacg aatctttagg taacgaataa acaataaaga gatatgaaaa
     5761 gccgtacaaa gaagcattcc ccattttggg atcactagcg gttgccatta atatgctgct
     5821 tagtaaaata aaatgaatca acatgcccat aactgaccat ttgatcagct ggacatacgg
     5881 tgctactaat agtgagaaca tgacgattgc taaacctatg atcgaatggg aaatcttata
     5941 accaaagtca acaaaacgaa tactcatcaa catacagaat aaaaccacag cgtatggttt
     6001 atgcttcgat tcaaataatg ttgtaattat aatgataaaa caaatggcaa acaatacgat
     6061 gaataatgat cgtaaaaaca atgaacaaat atacatgcac ttttcttttt tagaagctgc
     6121 ttctcgtatt ttttgtttta aaataaacgg atctagttgt agagactgat aaaaatccac
     6181 ctgttgtcgt cctttctttt tacttttatt ttttacagtc atctatgaca ggttgtaaat
     6241 actatggaac atcatatggc gttgtcattt tcttgtcaac tatttttcct attcatgttt
     6301 ttataaaaaa acatatcgtt ttcttgtatg attaaatcac cacaaaataa acaactgaga
     6361 gaacgatatg ctttatgaat ggttctatca aaaaaattct gagatcaata gacaaaaatt
     6421 tgatgattac agaggtctct tacgaaacct tacagaagaa aaaaacattg atcgttgctg
     6481 cagttctctt ccctgctcca tgtgtctgta gaaattgtgg ttctgctgtg gtagatggaa
     6541 ataggaaatt aatggtggtg aaaaacaaga agaaggaaac cattgtccac tttgaataat
     6601 ataatcatat gccaatggtt atacgcctaa aaaagcagcg ctatacctgt aaaaactgtc
     6661 gaagccattg gaacgctcaa agttatttta tccgtccaag gcactcgatt tcaaatcatg
     6721 tcagacataa aatcacttct ttactgactg aaaaagtgtc gttatttttt atttcgaaaa
     6781 gttgttaggt atctttgaca accgttattc gcacattgaa agagttgaga agctatttac
     6841 caaagtcttc caaaagaatt ctgtcttgag tgttgagggt tgtgaatttc gttctcacgt
     6901 ttccgttgag gataaactga gttttatttc cgcggatggc gagacagaaa aattaatcga
     6961 gaaaatttac ctacacaaaa aattcctcga ttaacaaact atttccttag ctgcactaat
     7021 cctaaagaaa tagaattctt agttacagac atgaacgcca cctactttca gctcaccaaa
     7081 catgttctac caaatgcgaa attggtgatt gatcgattac ttggtttttc cacgcctttc
     7141 aaggaggcgt aacctttatt cacgagttgg ttgaagcttt ccgagacaaa agacccgact
     7201 tgtttttctc cttattgaca aagagccatt aaaactagag aaaaaattac aactgatcgc
     7261 tgattatttg tagaaagaaa atcaaagtat tacccaataa acttgagtca ttaggcaaaa
     7321 aacactgcga aactcaagaa tgattttcgc agtgtttttg tatatttttt attaactagt
     7381 attactacaa aggtttattt ttcacttctc aacgcagcac aaaattgtct ttacttattt
     7441 actggttggg ttcaaccttc tctactccga tactaatatt tgtttgtata ccccttctaa
     7501 aatcggtagt ttccattcaa ttgatgggta ctagttgatt ttaaaaacta tgcttattca
     7561 attttttatt ttagctcata tatttcgttt ctttctaaat tttatactga tgaaaacaag
     7621 tagcacaccc aatattgaca atataaactg gctaatgatg gtttctccag tttttggaag
     7681 atgcttattt ccactgcttg ttggattgtt tcctcttata tctgagctat cagcaacttt
     7741 tctattctct tttgttaaaa agatcgccac ttcttgttct ttttcgatct tgaaagttac
     7801 tggtgtcgca tc
//
LOCUS       DASJTC010000104         7766 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.107, whole genome shotgun sequence.
ACCESSION   DASJTC010000104 DASJTC010000000
VERSION     DASJTC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7766
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.107"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..107
                     /locus_tag="U4B19_002388"
     CDS             <1..107
                     /locus_tag="U4B19_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HEN2078082.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="U4B19_002389"
     CDS             complement(176..904)
                     /locus_tag="U4B19_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2078083.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="U4B19_002390"
     CDS             complement(917..2197)
                     /locus_tag="U4B19_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEN2078084.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="U4B19_002391"
     CDS             complement(2338..2940)
                     /locus_tag="U4B19_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEN2078085.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="U4B19_002392"
     CDS             3185..3391
                     /locus_tag="U4B19_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078086.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="U4B19_002393"
     CDS             3703..4422
                     /locus_tag="U4B19_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEN2078087.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="U4B19_002394"
     CDS             complement(4503..5186)
                     /locus_tag="U4B19_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEN2078088.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="U4B19_002395"
     CDS             5405..7591
                     /locus_tag="U4B19_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEN2078089.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2784 a         1309 c         1465 g         2208 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgacaa
     7741 gaattttgtc acacctccct tattta
//
LOCUS       DASJTC010000105         7714 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.108, whole genome shotgun sequence.
ACCESSION   DASJTC010000105 DASJTC010000000
VERSION     DASJTC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.108"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..942
                     /gene="sgrA"
                     /locus_tag="U4B19_002396"
     CDS             <1..942
                     /gene="sgrA"
                     /locus_tag="U4B19_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEN2078090.1"
                     /translation="LGLSIIAAMIGISTSANERANENLSFTVKTDRIVYDMTQQVITI
                     PVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEYTIMISAELSID
                     EIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESSTENTSGESSTES
                     TSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMSSESSTGSTSSE
                     SSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSD
                     LPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            1240..1596
                     /locus_tag="U4B19_002397"
     CDS             1240..1596
                     /locus_tag="U4B19_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078091.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            1599..2756
                     /locus_tag="U4B19_002398"
     CDS             1599..2756
                     /locus_tag="U4B19_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2078092.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(2782..3222)
                     /locus_tag="U4B19_002399"
     CDS             complement(2782..3222)
                     /locus_tag="U4B19_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEN2078093.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            3382..3888
                     /locus_tag="U4B19_002400"
     CDS             3382..3888
                     /locus_tag="U4B19_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEN2078094.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4056..5969
                     /locus_tag="U4B19_002401"
     CDS             4056..5969
                     /locus_tag="U4B19_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEN2078095.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            5974..6975
                     /locus_tag="U4B19_002402"
     CDS             5974..6975
                     /locus_tag="U4B19_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEN2078096.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(7177..7383)
                     /locus_tag="U4B19_002403"
     CDS             complement(7177..7383)
                     /locus_tag="U4B19_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEN2078097.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2853 a         1158 c         1566 g         2137 t
ORIGIN      
        1 cttgggctga gtataattgc agcaatgatt ggaatcagta cttctgcaaa tgaacgggca
       61 aatgagaatc tttcatttac tgtgaaaact gatcggattg tttatgacat gacacaacaa
      121 gtcattacta ttccagtcaa accaaacaaa agcgtgaatg ctagtgatgt acatgctgtt
      181 ctaacatatg gttgggatgg taatggaagt tcagaaaaag ttattgggga agtttattta
      241 aaagacgtac aatggaccgc tggtattgag tatacaataa tgatttctgc agaactatcg
      301 attgatgaga tcaagagtaa agataaagtt gatttgattg ttttttatga tggacagatg
      361 acgatcacgg agaatttgaa accaagcagt tggacagttg ttggaccgcc aagcagtacg
      421 gatagcacaa gtagtgaaag cagtacagaa aacacgagcg gtgaaagcag tacagagagt
      481 acgagcagtg aaattagcac agaaaacacg agcagcgaaa gcagtacgga gagtacgagt
      541 agtgaaagta gcacaggaag cacgagcagc gaaagcagta cggagagtac gagtagtgaa
      601 agtagcacag gaagcatgag tagtgagagt agcacaggaa gcacgagcag cgaaagcagc
      661 acaggaaaag taaataatga acttgatttg gtacctatca taccaactaa ggaacaaaag
      721 agaaatgcag ggagcaacct aaaaagtgtg ctaaaacagc ctgattttgt ttctcgtgga
      781 attgatcatc aagaaaaagc gaaagaaaca gataatagaa aaagcgattt accagaaact
      841 ggtagtagaa ccttgaacaa gctaattaca tggatgggtg tgttactcat tttgattgta
      901 ggagcaagct attttcgttc tctcttccat cgagtgaaat aaagaataaa agcaacagat
      961 ctaaaattga ataaaacgat agatttttga gtgaatattt cattcgagag tgctgtattg
     1021 cctggcgaag ttgtagcttt gccagatcta tttttgttta ctaacatatt tgagaaacag
     1081 gaaataacag gaacacaaat ggaaaataag agcaagaata attgacaatg cacactgcgc
     1141 atggtagtat ctctgaaaaa tcagtaacga atctatttgt acgttattca tgtagctgtc
     1201 ggaataagtc taaataatag ataaaaagga gtatgtactt tgtttgaaga atttattgat
     1261 atcaatgaaa ggcaagtata tcaatttttg aactattgtt atgaaagaga cgagaaatta
     1321 tatgtggtga aggatatcgc tttagatcta aactacaccc tggctaagat gaattcggtg
     1381 attcaacaag cagaatcgtt ttgcgaaagg tatccagaat ataaattatc ttttctttca
     1441 gaaaacaaga tgataaaagt cgagttttct tctcagtttt tactgagtaa agtttattct
     1501 atacttttag agggaacgat tggttatatt ttactggatt cactttacaa aggtacttat
     1561 caatccttag aaaatcttag tcaaaaaatt atataagttt gcgtacgatt caaagaaaat
     1621 taagagaact taatactatt ttgaaaaact atcatttatc ttgcagcttg aaaaaaggta
     1681 atccgataga aggagaagaa taccgtatcc gatacttttt tcacttgatg tattggcagt
     1741 cattcgacga aaatcattct gatatattat caagtataaa aaataaacgt caactattag
     1801 aaaaatttgc gaaacatgcg ccatatacgc gtagtataga tagaaagaaa tggatcagcc
     1861 tactaagaat ttcgttgcaa cgaatgaaac aaggccactc gattacgtat gtcccagaag
     1921 agatgaacaa atttgtgcat ccattgataa caaaagaaga gtttacgcat tacatactag
     1981 agcctttttt tgaagcaaac ttctggtcga tgaatcacat cactccaaca gagcaagctt
     2041 acctttattt tatgtttggt gtaatgaaca cctatttgga agaagatttg ttggatactc
     2101 cagataaagt acaagaaagt tggggctgga ttgaatcgtc tattgtagaa aattttgaaa
     2161 aaaacttcaa gatttgtttt acaacttcag aaaaaaatta tctatatgtc aatttggcaa
     2221 tgatccattt gtattcaagg gtttttggga caaaaaagaa ggtggatgct tttggaaaat
     2281 cagcagaaga cagagatttt cagcatatat ttccagtgat gtatccaatc atgcaacagt
     2341 tttttagtgg ggtagctcgc agaattccag agcttaatac ttattatgaa aagaacagac
     2401 ggttgatttt tcaatactgt atgttagtca gggatatttt tataaaacac gaacaacctg
     2461 tcatattcta tattcaatca aagtatggta aaacacagga attatgggca aagaaaagga
     2521 tattatctat gactgaacgc ccaataaaat atagtgcatc cgctgatcag ttacctgata
     2581 ttgtcattag cgattatcct atcgacaaaa aggcatatga tcaaacgaat acacagatat
     2641 tttactggaa tggacaacct acaaaaaatg attgggcgcg tctgaaagct gaaatcataa
     2701 aaatcaggaa tgagaaaaat agatccatat tggaaaaatt ggatttcgtt aattaagaaa
     2761 agcagattgt ttatgccaaa attaagacat tgcaaaaaga ttattttctt taaaatcgac
     2821 aagatgacca gaaagcaaga gttcgttgag ctcttgcttt cttttagttt tagaaaggaa
     2881 catcctgatt ttatcttcgc taaatccagt caaaagtttt gcagattggt cgtagataat
     2941 cggttttttc aataaagaag gggatgtacg aatctcaata tatagttcac tcaaactgag
     3001 ttcatctatt ctgtgaacaa gatttttata actaatagaa tgagtagaga gcatttcggt
     3061 cacgccgttg tttgtcaatt ttagtaagtg gataaattca tttttggtta gtggttgacg
     3121 aattaagttc tgttcgataa acggtatatc atggacgata agccaacgtt ttgcgcgtct
     3181 tgaagacgag cagccagaac ctgtatacaa tttaatcatc atacattcct ccaatagcta
     3241 agtatttatg aaataataac atgaagaaat agtcgaaaag tgccatttag aggtgtaaaa
     3301 taagaaaatg ttgtcacgta taatggaact agctagataa gaaatactat taaatcaaga
     3361 aaaaagaaag aaggcacaag tatgtcaaac acagccgacg caatagtagt gatcgattta
     3421 caaaaagggg tatgttttga ttcttcgccg atacacgaat tagaaacagt gacagcatta
     3481 gtgaataaaa gaatcaaaac atacgaaacg attgggaaac cagttatttt cgttcaacac
     3541 gaagatgctc aattagtgcc acaaacaaaa ccatgggagc tattgtcgga attatctatt
     3601 cctgagcaca gttattttgt gagaaaaacc catgcgaatt cattttataa gactaatttg
     3661 agtgttttat taactgaatt ggactgtcat tcgatcgaat tatgtggggc acaagcagag
     3721 tattgtatag atacgactgt gaagtttgct catggattag gataccagct tatgatgtgt
     3781 agaggagcaa cgacaactta tgataatcct tttatgaaag caaaggaaac aatcgctttt
     3841 tacgagaaaa tttgggataa acgatttctg acatttttgg agcaataagt tgtttcttcg
     3901 tttaaaaaaa gcagaatgga tactggttta tccattctgc tttattacgt aaaatgagaa
     3961 aataatcctg ttctattttt atgggataaa ccatttattt tttgtataaa aaatgtttgc
     4021 gtttacatat aattctccta gaaacataaa aaggagtgaa tagcttgaga acaaaacaag
     4081 tattaaaggg agcgttggtc atcttatttg ctgtaagtac aagcgtcccg caatggacag
     4141 tatatggaga gagcatttct tccgaatcgg aatctcggga ggtagaaacc acagaaacta
     4201 ctgatgctgg aaatgaaacg aaatcgagta cttcacaaac agaaacaaat actgaaacaa
     4261 cggttgagac atctgttact agcacagatc aagaaacgac cgctaccaca gataaccaac
     4321 aaacagaaga agcaacagct acaaaaatgt caaacgacga atcctcttct gctaaagtaa
     4381 gtgacaaaga gaatgggaca gaaaatgatg atatcggtaa aggtgaaaat ttcaatggta
     4441 aagaatattt gaattacttc ggtaaacaga tgacagaaat acttaatgga ccaacagaag
     4501 acatcaagtc aaaattaata gagttattag tcactgagct tgctgttaat gattatggtg
     4561 tagataaaga gattatcaaa catctgattg ctacttatct attgtacgat atcgcttttg
     4621 caaaaaacag tgtgttgatg tcgatgcttg gaggtacaga tgatgggaca acgcctacag
     4681 aaaacacagg cttctatcca aatacaaaac aacttcatca aactgtacta aacaaacaag
     4741 atggcaaaat gatggatgta tatgcatatt atgtcgatca aggttcagat aaaacagtca
     4801 tgatccatgg cggatttcga gggaattgga ataacgggat cgttacagaa gaatacaatg
     4861 atttttataa ggccgggtat aatttattat ttgttgattc cagagcgact ggtaatagcg
     4921 gaggcgatta tgtgacctat ggacaatatg agtcagatga cgtcctttac tggataaatc
     4981 aagaagttcg agagcggcca tctcaaaaga ttttacttta tggtggttcg atgggagcag
     5041 ctacgatgat gtctgtatta gcgaaagata ttcctgtcaa tgtcaaagga attattgaaa
     5101 attgcggatt tgcatcaatt gatgaacaac tacgttttac gtacagccag acagtagccc
     5161 ccgcactacc ggatgccata aaaaatcagt tagacatcat tggggatcaa gagcatgaag
     5221 atttatttat gggccttttg aaacaatact attttgatca ggagatgcat ttggatacaa
     5281 aagctgctct acctacgatt ggaatgtcag attcattgcc taaattgatt atccatggaa
     5341 cagcagatga tgtagtacct gtgtctaacg cgcaaaaatt atatgagtta tcaggagggt
     5401 ataaagatct tttattagta gaaggagcag ggcatggaaa ggcacaggaa gttgaccatg
     5461 ctgcctacac gaaacatgta acggactttt tgaaagtagt gttccatgat cagataaacg
     5521 taaagtatgt ggatgaaaac aataaaagtc tactcaaaga ccaggacgaa atccagcttt
     5581 acggagctta tggagaaaat tatatgacag agcaaaagac attcgaagga tacgaattag
     5641 caaatgttga aggaccgact gaagggattt ttaatgagac gataccaact attattttct
     5701 attacaaaaa aatcccagta gctccaccaa aaaagcaaga tccgacagag aatgcagaca
     5761 ataagggaaa gaatgaacct cgagaggatc aatcaagtga tgatacgcaa ccgattcaga
     5821 tgacagtgaa agtacaaaaa aagacagatg caaaaaaaag aatgttgccg aaaactggag
     5881 aaaagaaaca ttcgcttctc atttattcag gtggattttt atctgttagc acgattatca
     5941 catggatttg gcgaagaaag agagggtaaa tgaatgaaat ggttcaagcg aatagtgatt
     6001 gcagtaccag ttatcatcgt agttatttta gtcactgtat ttttgattaa tcaaatcaca
     6061 ccaaagccga ttagcttgct tgttagaaaa caatttgaca caagtgaaaa agaacaagta
     6121 tatgcccgac cagatgattt ccagcaaaaa atcgaacaaa tcgatataaa aaaagaaata
     6181 tcctatcctt caaagtacaa aaataataat atggatatct acacaccgaa agtggaaaag
     6241 aaaaaattac cgattctttt ctggatgcat ggaggcgcat atgtaggagg ggataaaaat
     6301 gattgtcgtg attatttgga atatctatgt tcagacacgc agcaaatcat cgtaaatata
     6361 gattatgaac gatcaccgga agcaaaacat cctacaccag ttatccaatt gaacgaagcc
     6421 attcaagcag ctaaaaaaga gataaaagat caagcagatt ggtcgaatat ttcgattggt
     6481 ggaaattcgg caggtgcaca aattagtgga gaatatttgt tagcacttca aaatgaagaa
     6541 attaaaaaag ctgatcatct tgatccgact ttggagaaca agcagattac gaagtttatt
     6601 tctttgtctg gtctgttgga tccttctgca cttactcaag taagtgataa aatctctagc
     6661 tttctttatg aaaaatgtgg atgggcttac tttgataata aggactttga acaatcagag
     6721 aaggtcaaaa gtttagcctt ggtcagacat gcggatcgtt ggacgcaaaa cacattctta
     6781 acggatggaa atacaaatac gtttacgaaa caaatggaag aagtaacttc tgtatttgag
     6841 aaagcggggg tgaaagtcac aaaagtagat tatgcggaga aaaatgctgt tttgaaccat
     6901 gaatatcagt ttgatttcag caataaacaa gcgcaagaaa catatcaaaa gttagtaatg
     6961 tttttcaagg agtaaagtct tatgatgcaa aagaggttgt aatctgtcac ttagctccaa
     7021 gtcacaagga gcaatttcaa taaacaagct tttcatggtt tatgaatatt gtaacttgtg
     7081 actgaggagt tagagatggc aaggggcacc ttttattcga aatttgggaa tatcacacga
     7141 ttattgtcac gatctctttt acgtaaattt tataagtcag atttgttgtt ttgatcgttt
     7201 aaaaaaagca acaaatccga agcctgcgga ggcaagaagg gacaaaaagc cgagtggtaa
     7261 gcaaaagaat gctggttcca ctaatattcc gttgttattg atataaggct gggaacaaag
     7321 atgaatagca aataaaatca atgcactagc gaaaaaaagc aaagaaatat ggatagcttt
     7381 cataagcagc acctcctttt ttataattgt atgataaata gattaagaat ctataaatgt
     7441 gacaagtttg ttaggatgtg aataaaatga tcatctagcg aacttgaaac aaagatggct
     7501 aatgataagc aattaatgat tcagcaatca gataggtaca aagtttacca tcatacgtct
     7561 agaccaataa tagaaaatat gggaatataa tagaaaagat ttaggatacc cccaaaagaa
     7621 tttccccaaa aattcgatgt atcctaaact tttttatttt acataacaat catatcaact
     7681 gtgaaataaa attacaaatg ataacctaaa atga
//
LOCUS       DASJTC010000106         7601 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.109, whole genome shotgun sequence.
ACCESSION   DASJTC010000106 DASJTC010000000
VERSION     DASJTC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.109"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(223..948)
                     /locus_tag="U4B19_002404"
     CDS             complement(223..948)
                     /locus_tag="U4B19_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078098.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1102..1428)
                     /locus_tag="U4B19_002405"
     CDS             complement(1102..1428)
                     /locus_tag="U4B19_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078099.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1465..1830)
                     /locus_tag="U4B19_002406"
     CDS             complement(1465..1830)
                     /locus_tag="U4B19_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEN2078100.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1867..2082)
                     /locus_tag="U4B19_002407"
     CDS             complement(1867..2082)
                     /locus_tag="U4B19_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2078101.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2211..2546
                     /locus_tag="U4B19_002408"
     CDS             2211..2546
                     /locus_tag="U4B19_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2078102.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2622..4619)
                     /locus_tag="U4B19_002409"
     CDS             complement(2622..4619)
                     /locus_tag="U4B19_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078103.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(4717..4992)
                     /locus_tag="U4B19_002410"
     CDS             complement(4717..4992)
                     /locus_tag="U4B19_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2078104.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            5224..5958
                     /locus_tag="U4B19_002411"
     CDS             5224..5958
                     /locus_tag="U4B19_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2078105.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5997..7139
                     /locus_tag="U4B19_002412"
     CDS             5997..7139
                     /locus_tag="U4B19_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2078106.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2567 a         1385 c         1093 g         2556 t
ORIGIN      
        1 aaaatttttt cactaaaatc actacctgaa acagtctaaa acacttatat atcaacgttt
       61 aatgaaacta tctccgtttc atttcagata caaataaata tagtcgggct ccaaatccga
      121 aaaaaaatac ccccagtagc ttatcctaag ctactggaga tgtataagta cggtcatata
      181 acaacggggt agggtaaata aatgaaaaag atggaagaat cattattcaa taactttttt
      241 aaatacttcg ttgtaatcag ttacctgtcc gttcttaaat accacagaat aatctttttt
      301 cccattttca agtaaccgaa gtggaatatc tttaccaact ttctctgtca ctgcattact
      361 gatacctgtt agattagcta ataaatctga taaagattga gcatctgtag aaatctctgt
      421 atcagtattt atcgtgattc ctttatcttc atttacaagt ttccaagtag gtggtatagc
      481 tgtgtatgtt tctacaaatg aattaagttt ttcattaagt gacttctctt ttatatttcc
      541 atctaaaaac tctgtatata tggcttgagc tatactaaaa caaagctggc cttgttctaa
      601 taattttaca tcattagtta gtaaagctgt gttcacgtta tcaatcgctt caagaagagt
      661 atctttggtg tccctaacag ctttaggtgt gccttctgga atttccctac ttgatacctc
      721 gtccatttta tcttccataa accccttaaa atcatatctt tctcccaaat ccatagaagt
      781 ttttgagaaa gctttcattt ctgagtctat ttcttcaata atatttaaca tgtctgatac
      841 atattgttta ggctgattcg tttttaatct atatgtggta aatatctcaa ttcctccaat
      901 tatcaataaa attgccaaac aaatccataa taattttttc tttttcattt ttcctcctat
      961 acactgctgt tataacagtg actaatgtaa agattttccc agcaatcatc agactattat
     1021 ttacagggaa tcaaaaaaac tccttagtaa gcttacacct ttaggagttc ctgtttgaac
     1081 ggtatgcata atacatttaa atcaatttaa cattaacacc tcagtatatg gtatctctac
     1141 atatacacgg tctttttttg gatagtactc gacatttata ttgatagcac ttccattgtc
     1201 taacgtagct ttagcccggt acgtgttatc accattatca tgagtgatcg tcacatcatc
     1261 acatgtccga tttaagttat actgttcttg taaaatctga gttaccaatg gtacagatga
     1321 cttctctagt ccagatgaac ccgtcattct tgaaaaaatt atacctataa tagccaatac
     1381 aacgatacct ataatgacaa acttcttatt catatttact ttattcaact ttttatctcc
     1441 tatctccaaa atgattttct ctcatcagta actcattgac atccccaata gtacaccaaa
     1501 gatggctcca gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttctctcg
     1561 atataagtaa cccataataa cagcaacagc accaaatacc acaggaataa atgctaatga
     1621 tactaccata gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac
     1681 gatttgttcc ttcatttcat taattgtctg ctcagtactt ttctttactg tgtctgttcc
     1741 ggttgtctca tctgtttctt gtaatgctcc gcaaaactgg caatacttac tttcatttgt
     1801 tatttcttta ccacattttc tacaatacat gtcttctcct cctaaaatca ttcaattgct
     1861 tattagctaa ctgtttactt tattaaatac atcagaaaac agctcattta ttggtacact
     1921 taatatctca gaaagcattt tcatttgata gggtttagga atcgttctcc ctgtttgcca
     1981 cgaacttaca cattgctgtg taacattcat ttttttggct aactcagttt gggaaagtcc
     2041 tttttctttt ctgtatcggc tgagattact tacttttttc atgattctcc cctcctgttt
     2101 tattatatac aacaatatga tgtatttcaa ctcataaaat atataaatac ttcaaatgtc
     2161 attacaaata caacaacttg ttgtactcta ctgagaaggg gggtgtatta atgaaacaga
     2221 agcaatatgc aaaggtcatt agagaaaagc gaaaagcact cgggtggaca caaaaagaat
     2281 tagctaaaaa aatcttctct acacaacagg cagtagcaag atgggaaaat tctgtaacag
     2341 aacctaacct cgatagctta acagctcttt ctagagccct aggaacccct gtaagccatt
     2401 ttctagacaa tgttgacata gattatgagg aagagttttt ggctctctat cgctctctta
     2461 gtactgaaga tgcaattcga acaattgact atatgaagct attaaaaagg caagaaaatg
     2521 aacgcaatca gctattgaaa gattgaaaaa taaataaccc accgaacttt aacggtgggt
     2581 gtattgagat gaaataagat taagagctat ggataacctg tctagtttaa tataattggg
     2641 tctcttctat tccttactct ccgtccatca tctcttgtta cacttgtaaa ttgataaata
     2701 ggaactaatt caactcctga taatatcaat tcttttatta cttcatctat ccctgcccct
     2761 ttcgctagat atactaaaaa actactgctt gctttcacat tgtatagttt cttaatatcc
     2821 ttagaagctc gttcaagagt gaatccttgt ttaacaaagt agcctctgtt ttctattaaa
     2881 gcatttttca cgctgttatc cctgacattt cgtaggcgtt cacaaacagt gttaataagt
     2941 tctttttctg tcattagata tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct
     3001 tcatctaaaa tcaattgttt ttccttgcta agttctgatc gggaattttg aggaaatact
     3061 cttttagcct ctttttcgtt aaaacatatc ttaacccatt cataagaaaa atattgatat
     3121 gtaaaatgaa atttttccag aagtggaagg atatcttcat tcaatttttg aatcacagaa
     3181 gttgcattaa aaacgtctaa tcgatagaca ttgcttctct tatgtttaaa gtttcctaac
     3241 gctttctctt cctttaacct atttaggtat tcgagcatct tcaaaggaac atcttcatca
     3301 tctaattttt ttatcaatcc tataaaagtc ataagtgata gtaacttatt acatttatct
     3361 atgtcataag ttttaccata gtaagaactc atttttttag ctaattgttc ccccgacaaa
     3421 atacaagtca cactttcctt actatcatca aaaaaattaa catcagaaag caagacatca
     3481 tagacacgtc gcatttcctc tagataattc cttattgtcc tgaaaagttt gtctttcatt
     3541 gattcaagag tgtcaaaaag tacttttgat tcctctttca attttcgaaa atgtgtagga
     3601 tacaagtcta ggttctctaa tagccaatta aatgtttcta cccgactgtc tttctttaaa
     3661 attagcatca caactccaat aaaattttca ctgatatcac aattactgga atgacaataa
     3721 aaaatggaat gcccagtttt actggtaaaa atactagcag agggattatg atcgttgtga
     3781 aacagacatc ttaagttttt acttgactta agtaatttat tcatatcaat ggttaacaat
     3841 ctttcataaa tctcattaaa agacatgtcg gaggaatcaa acaggcagtc tttaaaccac
     3901 tgtttcattt cttctttgtc gtcatttttt atcaacttaa caaaatcggt ttcattgact
     3961 agttcatact ctctccctga atcactttga caatttttag aacatgtcat ggtaaaatca
     4021 atcggaagct cagtaatatc taacatgttg tcttcattaa ttattatcac tgcatctgac
     4081 ttatatcctc caaaaaacat tctattcgaa gcatttacat tgtcatcaca cccggggatg
     4141 ttctttttca agtattcgta aacagcaacc attttaccat agtcagtaat tgcttctttt
     4201 aatagaaaga caatcttgta tttttctaga ttttcagagt accgaatact tttttgtatc
     4261 gcaaacgcag aattatgaac taaattgata gataaaacct tttctatgct taagtatcta
     4321 ctatcatctt tactcaaatc tactaccttc cccttctcat ctttaacagt gttatcgaca
     4381 tccagaataa ttactctggt ttccacaaaa ctctccttgt cattgccttt attctttgtc
     4441 ttgcttggaa taaatgattg tgatgaattt agtatctcta caaactcatc tcttgtaact
     4501 tctctatagt tatctaaaca cctatctctt acatcagttt tttctttttt tgtaactggc
     4561 ttctctaata atttttcttt atctacataa attggaatat taatcattga tgtttccatt
     4621 tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca tacagataat aaaaacaaac
     4681 tctttattac tatatgtttt agtcttattt gtttactcaa cctttatacc gttccatgaa
     4741 actatcaata tcagattttt taaatctaat gaccgtatca atttttataa caggtaaccc
     4801 gtattgagaa acatattttc taatcgttcc tactgataca cctagatatt cacttgcctg
     4861 ctgatagtcc atatactcta gagtgactcc agaaataatg tcttctagca taggctttag
     4921 cactgtaatt atctctttca cttgttgttc aacttgtata gaaacaatgc tatcaatgat
     4981 ttgtagagac atatttcccc ctcctttcgt gattgcttga acaaattttc taagaattat
     5041 cttcgtattt tttgctcaaa ataaaagtat tttgttatta accgattaat caaacaaaaa
     5101 atgattacca gttcatcgac atacacataa tacctcgata aaaaaggggt gtcaatagtt
     5161 ttgtaaaatt tatttacaac accccttttt tgaggtatac taattttagg aggtgtggtc
     5221 aatatgataa aacgtccacc aataaattat ttagaacgaa aaaagatact cggaacaaaa
     5281 ataaaagcta taagaaagag taaaaaatta acacaaccag catttggtct tatgattaac
     5341 aacggtcaat taattgacaa aaagactatc tacgaatggg agaagggaac ctatttacca
     5401 ataccagagc gattatcccg aattgctgat ttgggaaata tgagtataga ggagttagtt
     5461 tgtggcaatg tagaagaata tattttggga attattcttt atagagatag cattgtacta
     5521 gatggtataa catttccaga taaaaacctg ttccagcatc ttcgtcaaca gttcccacca
     5581 gtacactcta atttagatac atggctggat agatattcca aattagaacc agagatgcaa
     5641 gaatttattg ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat
     5701 atcttaaaaa tagaagaatt attcatcaat gctattgtag aagaatttga caataacatt
     5761 cttttcctca ctagcagtat agaagaacta ctagaaagaa tggtagatga atggctacct
     5821 attcaattga aagacatgtc ttatcccgaa gaagctgtga gagaaattac tgataatata
     5881 aacaagctag aacaaacgat ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag
     5941 ggaggtgata ccatctagtg gtaagtactc cttgtaaact aagaggagat gatttcatgg
     6001 caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca
     6061 ttgccaccga tccacgaact ggtgaaaaaa taaatactac gagaagaggt tttagtagca
     6121 aacaagaagc tcaaaaagat tatgacgagt acagacatca aatattatac ggtgttaaaa
     6181 agaaagcacc tgatatgaca tttgaagacc tgtataatga atggataact catcagcgta
     6241 ccagtgttaa agcttcaaca atcgcaatct cagtaagata cgcaaataat caaatacttc
     6301 cagcatttgg taagcttaaa ctatcgaata tctctgtccc atattgtcaa aaagtggtag
     6361 atgaatggca cagtaaatat gaaagttacg attatatgag aaaacagact gcacaaattt
     6421 tacgctacgg ggttgcaatg caatatattg acaataatcc tatggaaaaa acacttcttc
     6481 cacgaaaaaa agagtatgag aaaaatcgta aattttactc aaaagaggag cttaataatt
     6541 tactggatgc ttttaaagat tttggtaaca tgaaacaata cgccttcttc agattattgg
     6601 catacacagg tatgagaaag agtgaagtac tcgctttaca gtggaaagac atagacacgt
     6661 ttaataaaga actacatgtc aataaaactc ttgctgtaga tgaatttggt aaagttataa
     6721 ttcaatctcc gaaaacaaga gcttctagac gagttatctc tttagatact gagactcttt
     6781 ctatcctcaa caactggaaa ttacagcaaa aagaagagta tttaaaactt ggatacaata
     6841 ctagctctaa agagcaacat gtttttacaa cagttaaaaa cactctatac attccaaaca
     6901 ctgttaatga ttggttaaga tatattttga aaaaatataa cttgcctcgt attacacctc
     6961 atgggtttag acatacacat gctagtttat tattagaagc aggagaatct gtcaaggtag
     7021 ttcaacaacg attagggcat gaaaatagta aagtgacatt agacatctat gcacatatta
     7081 caaataatgc acctaagaaa acaggtcaag actttgctga tatgatggct catcagtaaa
     7141 agagctggtc aaaatctggt catttctagt atacaggcaa aacaaaaaag ctagaaccct
     7201 tatatatcaa gggttctagc tcctataaca ggtttaccca acactgcctt ccaatgactc
     7261 ttttgggaat cagaggaaag aatcaggctt tatagagata tttcatctaa caaattactt
     7321 tagactgaca gtatatatca gcaggtactg tcatcgggaa cgtagccaat atcgtatcca
     7381 aaacagcagg tagagaagct atttgaggat tcaagttatt ctatttcaac catgaagtta
     7441 agttcattaa aaataaagat agttggtatt tcaataattt ttgtatagag ttaatcactt
     7501 tattgatgct aattcatctt gattggtatt ataatcgtat tcaagttgcg tggcgtgctt
     7561 ttgctagaaa atatataact gttctttata aagaaatata a
//
LOCUS       DASJTC010000107         7564 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.110, whole genome shotgun sequence.
ACCESSION   DASJTC010000107 DASJTC010000000
VERSION     DASJTC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.110"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            67..2676
                     /gene="clpB"
                     /locus_tag="U4B19_002413"
     CDS             67..2676
                     /gene="clpB"
                     /locus_tag="U4B19_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEN2078107.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2827..3513)
                     /locus_tag="U4B19_002414"
     CDS             complement(2827..3513)
                     /locus_tag="U4B19_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078108.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3491..3985)
                     /locus_tag="U4B19_002415"
     CDS             complement(3491..3985)
                     /locus_tag="U4B19_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEN2078109.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(4005..5225)
                     /locus_tag="U4B19_002416"
     CDS             complement(4005..5225)
                     /locus_tag="U4B19_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2078110.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5372..7207
                     /gene="lepA"
                     /locus_tag="U4B19_002417"
     CDS             5372..7207
                     /gene="lepA"
                     /locus_tag="U4B19_002417"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEN2078111.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7403..>7564
                     /locus_tag="U4B19_002418"
     CDS             7403..>7564
                     /locus_tag="U4B19_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2078112.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLS"
BASE COUNT         2509 a         1423 c         1538 g         2094 t
ORIGIN      
        1 tataatagtt tttgtaaaga tcaaagaaat aagtgatctt acctttaatt aggagggtat
       61 acctgtatga atattgagaa aatgactacg acgcttcaag aagcgattgc tgaagctcaa
      121 cagattgcgg tcactcgaaa acatcaagat atcgacattg cccatgtatg gaaaatcttt
      181 ttacaatcta atcattttgg tcgaaatttc tatacggatg ccggattgga tgtcgaatct
      241 tttgaacatg aaattgatcg tctactggat gagtatccag ttgtttctgg tggaaacgtc
      301 caatatggac aaaacctcag ccagaatttc tttcgattat tgaatgaagc agaccaaata
      361 cgtgaaagct tcggcgacga gtttctttca acggaagtcg ttattttagc gttgatgaaa
      421 ttaaaaaatt atccgttaac tgtttatttg aataaaaacg gattatctga aaaagaattg
      481 cgtaaaaaca tcgaagagat gagaggagga gaaagagtga cctcacaaaa ccaagaagaa
      541 caatacaaag cattagaaaa atacggcgtt gatttagtac agcaagtcaa aagcggaaaa
      601 atggatccaa tcatcggtcg tgatgaagaa atccgtgatg tcatccgtat tctttcacga
      661 aaaacaaaaa ataatcctgt tctgatagga gaacccggtg tagggaaaac ggcaatcgtt
      721 gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg aaaatttaaa agataaaacg
      781 attttttcat tagatatggg tgcattgatc gctggagcaa aattccgtgg tgaatttgaa
      841 gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg atggacgtat cctcttgttc
      901 atcgatgaaa tccacaatat cgtaggggca ggaaaaacag aaggaagtat ggatgccgga
      961 aatctgttga aaccaatgct tgctcgcggt gaattacatt tgatcggtgc cacaacttta
     1021 gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg aacgtcgctt tcaaaaggta
     1081 ttagtcaaag aaccaacagt ggaagatacg atcagtattc tgcgtggatt aaaagaacgt
     1141 tttgagatcc accatggtgt taacatccat gacaacgcat tagtagcagc agctacttta
     1201 tctgaccgtt atattactga tcggttctta ccggacaaag caatcgattt gatcgatgaa
     1261 gccagtgcaa cgattcgggt agaaatgaac tctatgccaa cggaattaga tcaagttact
     1321 agacgtttga tgcagttgga aatcgaagaa gctgcactga aaaaagaatc agatgatgca
     1381 agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg aacttcgtga agaagcaaat
     1441 gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca attctgtttc agcaaaacgt
     1501 gctgaaatcg ataaagcaaa acacgaatta gaagatgcag aaaataacta tgatttagaa
     1561 cgcgctgctg tattgcgtca tggtacgata cctcaattag aaaaagaatt gaaggaatta
     1621 gaagctaaag caaaagacag cgagattaag atggttcaag agtccgttac cgaaaatgag
     1681 attgctcaag tcgttggcag attaacgggt atccccgtaa cgaaacttgt agaaggtgaa
     1741 cgagaaaaac taattaaatt gaatgaaaca cttcataaac gagtgattgg tcaagatgaa
     1801 gcagttgatg cggtcagtga cgcggtaatt cgttcacggg ctggattgca agacccaaat
     1861 cgtcctctag gttctttcct tttcctagga ccaactggtg tcggaaaaac cgaattagcg
     1921 aaagcgttag ctgagaactt gtttgactca gaagatcata tggtacggat cgatatgagt
     1981 gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag ctcctccagg ctatgtaggt
     2041 tacgaagaag gtggtcaatt aacggaagct gtcagacgaa atccttatac aatcgtcttg
     2101 ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata tcttgttaca agtcctagat
     2161 gacggacgct tgactgattc aaaagggcga gtagtagatt tcaaaaatac ggttctgatc
     2221 atgaccagca atatcggttc acagttactg ctggaaggcg tgactgctga tggaacgatt
     2281 ccagaagcag tggctgaaca agtcaacacg ctattacgtg gaaacttcaa accagaattc
     2341 ttgaaccgaa tcgacgacac gattttattt acaccattga gcctagacaa cgtgaaagga
     2401 atcgttgaca aaatggtggc gcaattggca caacgtctgg aacatcaaga aatcctgctg
     2461 acaatcagtg atgaagccaa aacgtggatt gctgaaaatg cttatgaacc agcatatggt
     2521 gcaagaccat tgaaacgatt tatcactaag gaagtagaaa caccactagc taaagaaatc
     2581 gttgcaggac atgttatgcc taaatcaaaa gtaacgatta ctttacttga tggacaactt
     2641 catttcaaaa cagaagagct agaagaaatc gtctaatctt ccattgagac tataaaagaa
     2701 aaaaggggtc tgacacaact agtgtcagac ctcctttttt caaataaaca gtgttccaga
     2761 agtagtttct tcgaaaaata agccgaaatt tcttgaagct gcccgcttct gtcacaacct
     2821 cttttattag gtatttttca ctttttctaa tttataactt tcaaagtaac gctcatcttt
     2881 tttactttga gaccatgcaa acaatcccat taaggcagca cctatcgtat ttgtcagcaa
     2941 gtcgcccatc gtatccatca atgctgctcg tccgacaaac agtgtgccat cagaagcagc
     3001 aaaacgttga agattcatgc ctaagaactg gtcgcataag aattcccaga actcccaaaa
     3061 cacgccgcaa agtccagcaa acgcaaagcc gaataagagg aacaaccaag gactggtttt
     3121 tgatatctca gcatctttca tcaagtaacc aatcaatcca tagccaagag ctgtcaggac
     3181 cattggactc accgcatgta agattttgtc ccaaaaagag atgatactga tcaaatgcat
     3241 ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag taaaacaaaa cgattgctgc
     3301 tggtatttta agtttgaaaa ctttcaaaat gacttgtggc atgaaaatca atgcaatccc
     3361 taataaagat tccacgataa atgtgattcc ttgagaagtc gaatattttt gtgtgataaa
     3421 ttcataaaaa ttatagcaga caacgaatac accaaaagca agtaaagcta tttgcacctt
     3481 tttgatttct ttaatcactg aaagtccctc catctaaaaa tctttttact tctgaaagca
     3541 actcaatttc tgaagaaacc acttttccgt tcaatttgat caaacctatt gtatataagt
     3601 tcaggtagtg aaattgattt tcagctgttt cttgtagtgc agcgattttt ttcgggtggg
     3661 ttccgccttg ctgtcgagta tcagtatata agccgatcac tgggatgcct ttcgcataag
     3721 ccacaccgat ttccgatgct accccggcat cgatcgtcag cccatctagt aaagcaatca
     3781 ttaaatcact agccaacact ttttctgtgt cagcaagagc aatcattcga ctgtctgcat
     3841 aagcagtttt gtcattgatt gcttcatttt cttgaggaag atagatcgaa agctcagaag
     3901 agagctctct gatttcttta actaactgtt ggttatataa caaatcactt ttagcaaaca
     3961 ttggtgcggc aaaataaatt ttcatctaaa catcatcctt tttatcaaaa ataatcgcta
     4021 aagtaagctt ccatgtttgg aatgatacgc tctagtgact tcagtgtttc ttcatctgtt
     4081 tctaaccgtt cgattcttgc caaataagaa gcacggcgat aattcatcat caagcaagtc
     4141 aatgtttgga tatccagtcg aacggatttt cctataggtt catttaagac acggacttgt
     4201 ccctgttcat cccatgttaa agcgaagaca ccattattcc aaggagcgac aggatcttca
     4261 atgatgaaat gaaaagcatc agctgttcca acaaacggaa accgttgaag gaattctttt
     4321 acatcaacga tccgagccat aaaatatggt tcgatctgct ctttgatctg actatcttca
     4381 atcaaaaatg ctaaaggctc atttttataa atatctccat gtacccagta gatcatggaa
     4441 aaatgtgcgg aaataaagtt ccataagcca tttcgagctt cttgatcaag atagaacatt
     4501 tcttttacgt gaaatatttc ttcagctacc cagtagaaca gaacacctct tggttcatgg
     4561 ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat tttcgaaacg ccaatactct
     4621 tcccaatgaa aatcactgcg gaataaagca ccatggtttt gttgggcaaa tttgtcataa
     4681 acggaaaata cgtctggatg atcaactggt aatcgctcaa ccataccact tactgggatt
     4741 tgtttaggta gctgtgtatc tctgatttta aaggaaagtt tatcagacat gatttcccat
     4801 cctttacgcc gatagtaggg gatactgtaa ggatacaaat aagagatcca ttgcttattc
     4861 ttgcgcatat tatccaatgc aacgataatt agatcttgca tcaatccatg gttagcatat
     4921 tctggataag ttccaactcc agtcacacca cccattttat aacgtgcacc gtgaatattg
     4981 acttcacaag gatagatagc gatttgagag atcagtttat tttcgtggaa ccatccgaac
     5041 actttcgata cttccaaaat cggttttttg gaacggataa aagctcgttt attctcaaac
     5101 ccgctttcct cgatgtctgc ttcagtcact tggaacacat aggaaagcaa ttcattgaat
     5161 tgatcgatat gttcttgagt gaccggtttt aattcaagat tttctctaaa atctttttga
     5221 tccatttttt catctccatt tattagcttg ataacagtat agcaaaattc tacaaaaaaa
     5281 acttctatag ctgactaatt aaaaactctt taaattactg cttttcgttg gtataatgga
     5341 aagtgctgat tatatgaaaa gaggagacgc tatggatata aagaaaatga aagaacgcca
     5401 agagcagatt cgtaattttt cgatcatcgc tcatatcgac catgggaaat ctacattggc
     5461 cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc gaaatgcaag atcagctgtt
     5521 agactcgatg gatttggaac gtgaacgtgg aatcacgatt aaattaaatg ctgtagaact
     5581 caattacaca gcgaaaaatg gagaaacata tatctttcac ttgattgaca ccccagggca
     5641 cgtcgacttc acctatgaag tctctcgaag ccttgctgcc tgtgagggtg ccgttttagt
     5701 ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc aatgtttatt tagcgttaga
     5761 caatgatttg gagatcctgc cagtcatcaa taaaatcgac ctacccgcag cagatcccga
     5821 acgtgtccgt caagaaatcg aagacgtaat cggaatcgat gctagtgaag ctgtattagc
     5881 cagtgccaaa tcagggattg ggattgaaga tattctcgag cagatcgtgg aatatgttcc
     5941 tgcaccaagt ggagacttag atgcaccgct aaaagctttg atctttgact ctatttatga
     6001 tagttaccgc ggtgttgttc taaatgtccg gatcacggac ggtatggtca aacccggaga
     6061 caaaatcaaa ctgatgagta atggcaaaac atttgatgtt acagaggtcg gggtcttctc
     6121 gccaaaagca gttgctagag actttttgat ggtcggtgat gtcggttata tcacagcaag
     6181 tatcaaaact gtccaagata cacgagtagg ggatacagta acgttagcag acaatcctgc
     6241 cgctgaagct ttaccaggtt accgaaaaat gaatccgatg gtttattgtg gtctttatcc
     6301 aattgatact tctcgttaca acgatttgcg ggaagcttta gaaaaactgc aattgaatga
     6361 tgctgcttta caattcgaac cagaaacctc tcaagcatta ggttttgggt tccgctgtgg
     6421 attcctcgga cttcttcata tggatgtcgt ccaagaacgc ttggaacggg aattcaatct
     6481 agaattgatc acgactgcac catccgttat ttaccatgtt aataaaacgg atggtacgac
     6541 tgctacagta gataacccgg cagattttcc tgaacctgta acgattcagg atgtagaaga
     6601 accattcgtc aaagcacaga tcatggtccc aaatgatttt gtcggtgcag taatggaact
     6661 ttctcaacga aaacggggag aattcattac gatggattat ttagatgatt accgagtaaa
     6721 tgtcgtctat aatattccgc tttctgaaat tgtctttgac tttttcgaca aattgaaatc
     6781 aagcacgaaa ggctatgcct cattggatta tgaaatgtct ggttatcaaa aaagcaagtt
     6841 agtgaagatg gacatcttgc tgaatggaga aaaagtcgat gcattaagct ttatcgttca
     6901 ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa aaactgaaaa aactaatccc
     6961 acgccaacaa tttgaggtgc caatccaagc agctattgga caaaaaatcg ttgcccgttc
     7021 agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt tatggtggag atgtttctcg
     7081 taaacgtaaa ctcttagaga aacaaaaaga agggaagaag cggatgaaac aaatcggctc
     7141 cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa atggatgagc aagatagtgt
     7201 gaaataactt ataatcaatc gaaaaaccta ttaaataaac ctttttgagt agatgcaaca
     7261 ctgaggatta tctgtataaa agattttaaa ctgtcatttg gttatcacgg cgatactaaa
     7321 attaacaaaa ttggaatatt tttcttgaag atttttagct gtcagtatat gatgaaagca
     7381 tctcatagga ggtgcttttt tcatggttga taattcaagt ggaagggttc ttaaatctct
     7441 aagaaaagag aaaaaactat ctcaaaaaaa gttagctgat ttagcaggta tttcacaaag
     7501 tactttagtt aaatatgaaa aagggtcaag aaaaatccct aaagacgtag ataatactct
     7561 aagc
//
LOCUS       DASJTC010000108         7561 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.111, whole genome shotgun sequence.
ACCESSION   DASJTC010000108 DASJTC010000000
VERSION     DASJTC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.111"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(75..257)
                     /locus_tag="U4B19_002419"
                     /pseudo
     CDS             complement(75..257)
                     /locus_tag="U4B19_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            244..441
                     /locus_tag="U4B19_002420"
     CDS             244..441
                     /locus_tag="U4B19_002420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078113.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(484..1059)
                     /locus_tag="U4B19_002421"
     CDS             complement(484..1059)
                     /locus_tag="U4B19_002421"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEN2078114.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1214..1740)
                     /locus_tag="U4B19_002422"
                     /pseudo
     CDS             complement(1214..1740)
                     /locus_tag="U4B19_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2197..3708
                     /gene="gadC"
                     /locus_tag="U4B19_002423"
     CDS             2197..3708
                     /gene="gadC"
                     /locus_tag="U4B19_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HEN2078115.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            3721..5121
                     /locus_tag="U4B19_002424"
     CDS             3721..5121
                     /locus_tag="U4B19_002424"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HEN2078116.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5286..6721)
                     /locus_tag="U4B19_002425"
                     /pseudo
     CDS             complement(5286..6721)
                     /locus_tag="U4B19_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7114..>7561
                     /locus_tag="U4B19_002426"
     CDS             7114..>7561
                     /locus_tag="U4B19_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEN2078117.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT         2371 a         1279 c         1377 g         2534 t
ORIGIN      
        1 aattattgac acaacccaaa cagggagcgt caataatttt gtgtaaataa ctcgtccttc
       61 tgcaaaataa tgggttactc agtaaacatt gaagctaatg tatccgtcac ttgttggaag
      121 cctttatggc ttctatttaa gaatttttga ttgtatgtat caaagatgga tactaggaaa
      181 cgctctagtg attcttcatt ttgaaactgc tcttttctgc ggctgtattt cttgatttgt
      241 ttattgaaag attcgattgt agaaccagaa ggaatggaga acacttttga actttacaac
      301 aaaactactg ttgataaatt aattccaatt ttagttccaa ctgatttaaa tggtcatgat
      361 gttttacttc gttttaacgg atttttaggt gaggattata gatacctaaa aatttctgat
      421 cctcaacctt taacagaata ataaaaaacg tgtaatcaat tatttgatta cacgtttttt
      481 atttcaatct tcgttgtagt agtggttgcg acataccgaa aggtaatctt cattacctcc
      541 tactacaatc tgttcacctg aataaactgg aataccatca acaatacgag cattcattac
      601 agccttttta ttgcaaaaat ggcaaatagt tttaatttcc tcaattttat cagcaaatgt
      661 caataatgct tcggatccct tgaagagatt attttgaaaa tcattcttca atccatagca
      721 aattactggt acttcctcat tcattacgat aagtgataga tcaataatat tctctctact
      781 taaaaattga gactcatcga ccaaaataca atcaactttt ccgtactgat caataatctc
      841 cactaattct ccgtacacat ccatttcaga agtaattgca atagctggtt tcgaaacacc
      901 tatacgactc tcaacttttc caaatcctga tcgagtatct attgcgcttg tcattatgat
      961 aactctcttc ccttgttctt cataattatg agccactttc agtagctcaa tactctttcc
     1021 actattcatt gttccatatc tgtaaaataa ttgtgccatt aaatttattc ccttctttta
     1081 caattcaatt attccttaat tccatctgct tttatgcagc tgctcgttta ttccttgata
     1141 tgtctataac atctactatc cctaaaacca atttataagc cattagagat ggtattcctg
     1201 aacatctctc atattgtttt ttgagtggta tatcatctga aaaactgaag gaatcatgaa
     1261 aatagcgaca tacttatcat ttagagttct aactttaaat gcatttactt cttcagtcag
     1321 cactttagtc atgttggaca tggtttgggg cgtgtagtga tgcccataca ttttctcaat
     1381 caagtctgcg atttccgaca tggtaatacc ttttcggaag aggtgaatga cggtctcctc
     1441 taatgtgtca ttcgtccgtc tataagcagg aacagtctgt tgcttgaact cgccattgcg
     1501 gtcgcgcgga atctggagat gaagttcccc atactcggtc ttgaccgtac ggtcatagga
     1561 gccgttacga gaattacccg tgttaaaacc aatgcgatcg tacttttcgt aatcgaggaa
     1621 agccgttaac tccgttttga gaagcgtatt gacagctttt tccaaatgga cacggaataa
     1681 tccattcaaa tcgcctttgt tcgctagagt ctttaggatt tctgtagtaa aatcgttcat
     1741 atggaagtcc tcttttctgt gaatgtgttg tggtaactct attctacaga agggacttcc
     1801 ttttttctat ttacacaaaa tattttacac tctcaacaaa agtatcttcg attaatactt
     1861 gctcaagtta tgtgcagcta cgaaaaagta ggtaccgagg tactttcttt ttcgcagctt
     1921 tttttggact tatgagaagt acattcgctg gaagaatacg atataaaacg agctttttca
     1981 ctaaagatat taatagagtg aaagaaacaa gcttttgttg tcagtttttt cacttttcta
     2041 aaaatgaaaa taaaaaattc tggaagtgga gaagaatttt gcttgctata ctttgtcttg
     2101 tagaaaagag gtagacaaaa taatgcaagc tcaaataagc gattttccat aaccttggta
     2161 ggaatgacaa attaaatagg aaagggaagt aataatatga ataaaaaaaa gttatctcta
     2221 ttcggttttt ttgccctgac agcttccatg gttttgactg tttatgagta tccgaccttt
     2281 gcaacatcac aattacattt ggttttcttt cttcttttgg gaggtatttt atggtttcta
     2341 ccagttgctt tatgtgctgc agaaatggca acagttgaag gatggaaaga tggtgggatt
     2401 ttcagttggg ttagtcaaac tttaggagaa cgttttggtt ttgcagctat ttttttccaa
     2461 tggtttcaaa ttacagtagg ttttattaca atgatctact tcatactagg agcgctgtcg
     2521 tatgtaatta attttccaga gcttaataca gatccattta tcaagtttat tggtgtatta
     2581 gtcattttct gggtactgac tttttcacag ttgggcggga ccaatcgtac cgctaaaatt
     2641 gctaaagcag gatttgtcat tggaattgtt attccttcga tcattctctt tggtttagca
     2701 gcagcctact ttatcggcgg taatccgatt caaatggcta ttaatgcgaa agcttttatt
     2761 cctgactttt ctcagctttc aactttagta gtatttgtat cttttatatt ggcttatatg
     2821 ggcgtagaag cttcagcatc acatattaat gagctggaaa atccccaaag aaactaccca
     2881 ttggcaatgt ttttactagt gattttggcc atcgccttag atgcaatcgg cggtttctca
     2941 gtagcagcgg ttattccaca acatgacttg tctctcagtg ctggtgtaat ccaaacattt
     3001 gaaacgctta ttttacattt taacagtcat ttacattggt tggtaaaagt gattgctctc
     3061 atgattgcct gtggcgttat gggtgaagtt agttcttggg tagttggtcc atcacgaggg
     3121 atgtttgcag ctgctcagcg aggtctgtta ccagttttct tccggaaaac taataaacaa
     3181 gacgttccag ttccgttgat tatgatacaa ggaattatcg ttactatttg ggcagcagtc
     3241 ttaacttttg gtggaggagg caacaattta tcattcttag tagcaatctc cttaactgtt
     3301 gtcatttatc tcgtaggata tttactattt ttcctaggtt actttgtatt agtttttaag
     3361 aaaaaagact taaaacgaac ttacaatgta ccaggaaaaa ctgttggcaa aacgattatc
     3421 ggtggaattg gttttatttt atcagtgttc gcattgctta tctcttttgt accaccagct
     3481 tcaattgcag ctagtgagca ccgaacatac caaatcatac tacttattag ctttgtagtg
     3541 actgttcttt taccttttat catttatgaa gtacatgata aaagtgctca cacaactatt
     3601 gaggaaccta ctcacgtgaa agctgaagac gttaatcccg ctgtttatcc agctgctaga
     3661 ggagagcacc acgttacaaa aaatgatgaa catattttaa aacattagga ggaattcatt
     3721 atgttatacg gaaaagataa tcaagaagaa aaaaactatt tggaaccaat ttttggctct
     3781 gcaagtgagg atgttgactt gcctaaatat aagttaaaca aagaatccat tgaaccacga
     3841 attgcttatc aattagtaca agacgagatg ttggatgaag gaaatgcgcg attaaactta
     3901 gctacttttt gtcaaacgta tatggaacct gaagcagtga aattgatgac ccaaacgtta
     3961 gaaaaaaatg caattgataa atcagaatac ccacgaacaa cggaaattga aaaccgctgt
     4021 gtaaatatga ttgctgattt atggcatgct ccaaataatg aaaaattcat gggaacttca
     4081 acgatcggct cttcagaagc ctgcatgctg ggtggtatgg ccatgaaatt tgcttggcgt
     4141 aaacgtgctg aaaaattagg tcttgatatt caagcaaaaa aacctaacct ggtgatctct
     4201 tctggttacc aagtttgttg ggaaaaattc tgtgtatatt gggatgtgga actgagagaa
     4261 gtcccaatgg atgaaaaaca tatgtcaatt aatctagata ctgtcatgga ttatgtggat
     4321 gagtacacaa ttggtattgt aggtattatg ggtattactt acactggtcg ttatgatgat
     4381 atcaagggtc tgaatgattt agttgaagct cacaataaac aaactgacta taaagtatac
     4441 attcatgttg acgctgcatc gggtggcttt tatgcaccat ttactgaacc tgatctagtt
     4501 tgggattttc aattgaaaaa tgttatctca attaattctt caggtcacaa atatggtttg
     4561 gtatatccag gtgtgggttg ggtcttatgg cgtgaccaac aatacttacc agaagaatta
     4621 gtatttaaag ttagttactt aggtggagaa atgccaacta tggctatcaa cttctctcat
     4681 agtgcagcac aactaattgg gcaatactac aactttgtac gctatggctt tgatggttat
     4741 cgtgatattc accaaagaac tcatgatgtt gctgtttatt tagccaaaga gatcgaaaaa
     4801 actggtattt ttgaaatcat taatgatgga tcagaattgc cagttgtgtg ctataagctg
     4861 aaagaagatc ccaatcgcga atggacacta tatgatttat ctgatcgtct gttaatgaag
     4921 ggatggcaag tcccagccta cccattacct aaagacttgg atcaattaat tattcaacgc
     4981 ttagttgttc gagcagactt tggaatgaac atggctggtg attatgtaca agatatgaac
     5041 caagcaattg aagagttgaa taaagctcat attgtttatc ataaaaaaca ggatgtgaaa
     5101 acgtacggct ttactcacta aaattcttca gagaaatata aaagaagtta attaactaat
     5161 aaaaggagtt gtgattacca cacagctcct taaatctatt tgaataaata tatgatattc
     5221 atttgttaca ctctcaatct tagctgttgt tctattcatc attactctta ttgtaagtag
     5281 ttcagtcaaa tttgcttgtt caattgatcg ataacagtat gaattgtttg aaaagtaatc
     5341 ggatatataa ttgttttctc actacctaag ccttcttggt ataaagaact atcgcaaagt
     5401 ataatccgct gagtacaaaa gaaagtgtcg tctactattt ctctaagttg tttacaaatg
     5461 atattactaa tagaaaagtg ttctcccaaa atgtcttcta actgttgtcg atagtataaa
     5521 tacctaaagt ctgacggagc tactatgaac acttcaattg gagatttccg tttatgctca
     5581 tttaaaagac ttaaactaat ggttagttta cgaatagaat tctcattcca acgcagaaat
     5641 aaattgtttt gttttcgcca ttgttcaaga atttcatgaa cttctaagta caaagcgcgt
     5701 tgctcttcat ttaacaagta agtcttatca ggttgaaata tctgaatatc agcccaaagt
     5761 gagcggataa acgggagaag tgctttatca aaaagtatct gattttcttc agatatattc
     5821 agagataaga taagtttttg actgagctca atcaccaaag ggttatctgc tacatggttt
     5881 tgataaacaa cgttcaagtc tttttgtaac aattcaaagt tatcgcatag ataatttcta
     5941 gagttgaata gaaagtagat ataaaggcat tcatcttcgt catgacataa agagaaagaa
     6001 agttgctcta atcctttctg tattagctga aacaagggag tatgtcgggc aactcttttt
     6061 tcttcctgac taaaatatat tgggttagtc ttagatctct gcaaaccgat agcgatacct
     6121 aataaaataa attgtttttc aactgtaggg taacttcttg aaaaaaatgt ttttctacat
     6181 aatcaatcaa ttgttgaccg ctttgaataa ttgatttatt tagctggatc aactctgtcc
     6241 gatttgtctc atagattatc gttagtaaca aacttcgata atccttttca ggaatttcaa
     6301 aatggccatc agcatttaag tactgcattt gtttaaaaaa attaatcaac tcatttcgta
     6361 agttgtaaac tttgcttaac gaaatgaagg tagtatcagc tatattactc caattgtgtt
     6421 cccctgataa ataatgtcct agtacattga aaaagtcaga gcgctgatag atgagttgag
     6481 tgagtgccaa cctcgaaaac gaagagtcga atgcgactgt aaagctactt ctagatttct
     6541 ccactgaaat aatttgctga tggagtatgt cggtaatgca ttccaagtcg ttagtgacag
     6601 tgagtgggca aacgttcaat tgatctgcta tttcgcgaat ctgaatggtc cctttttcgt
     6661 gtaattgctc acaaatgtaa acttgacgaa aaatactttt ctccagatag gtttccaaca
     6721 tagtactcct cctagtcatt ttatacataa ctattataac tctaccaatc ggaatagtcc
     6781 tgtaatatat tgaatcaaaa atataaatta taaaataatt agatggggta tttggtttaa
     6841 gtatccctta tatagtaata aatttatagg tgtgattctc gaattaatac ccgaaaatgt
     6901 gagagaattt ttatggattt tttaggatac aaattaatta tttttgcttt tatcctgatt
     6961 accagtccag tactgtcggt atcccatttt gcagcaaaat catgttgata aagtgacaag
     7021 aagactgatg agatatatta tccaaattga gaagataatt aatcggattc ttgagtatac
     7081 cctacttttt aagaaagtag ggtatacaag cgtatgaata aagacataac tttccctgtg
     7141 atggacgttc ggcttgttcc aattaccaaa atacaggcga acgattataa tcccaatcga
     7201 gtagcgtctc cggaaatgaa cttattggag ctttctatca ttgaagatgg ctttactcaa
     7261 ccattagttt gttattacaa ggaaaaagaa gagaagtata tcttggttga tgtgcttcat
     7321 aggtatcgtg ttgctaagga aagactcaac ttaaaagttg taccggtaac agttattgat
     7381 aaacccatag aagaacgtat ggcttcaaca attcgacaca atcgtgcaag agggacccat
     7441 gaaataagag gaatggaaat tgtcatccaa agattagtaa aaaatggatg gtcagacaag
     7501 agaatatcca aagaattagg catggacatt gaagaaattt ttcgctttaa gcaagtgtct
     7561 g
//
LOCUS       DASJTC010000109         7512 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.112, whole genome shotgun sequence.
ACCESSION   DASJTC010000109 DASJTC010000000
VERSION     DASJTC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.112"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            220..476
                     /locus_tag="U4B19_002427"
                     /pseudo
     CDS             220..476
                     /locus_tag="U4B19_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            863..1090
                     /locus_tag="U4B19_002428"
     CDS             863..1090
                     /locus_tag="U4B19_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEN2078118.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            1106..2272
                     /locus_tag="U4B19_002429"
     CDS             1106..2272
                     /locus_tag="U4B19_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEN2078119.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            2507..3550
                     /gene="hrcA"
                     /locus_tag="U4B19_002430"
     CDS             2507..3550
                     /gene="hrcA"
                     /locus_tag="U4B19_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEN2078120.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            3644..4207
                     /gene="grpE"
                     /locus_tag="U4B19_002431"
     CDS             3644..4207
                     /gene="grpE"
                     /locus_tag="U4B19_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEN2078121.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            4258..6087
                     /gene="dnaK"
                     /locus_tag="U4B19_002432"
     CDS             4258..6087
                     /gene="dnaK"
                     /locus_tag="U4B19_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEN2078122.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            6238..7404
                     /gene="dnaJ"
                     /locus_tag="U4B19_002433"
     CDS             6238..7404
                     /gene="dnaJ"
                     /locus_tag="U4B19_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEN2078123.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2571 a         1277 c         1666 g         1998 t
ORIGIN      
        1 gcttcttcaa aagtacatcc tccagtaaat tctaagtact ggaggattct ttttctagtg
       61 ttgctttact catgaaaaag tagttggcta cttcatcaaa atttctgaac ttttttaatg
      121 tataataaag agctcgatca tttactacaa ggatgctagc tcctacattt tcacagtttt
      181 ttagcaagta aatttttcta aaatcacatc aggaaatgca tgagattctt taagagaggc
      241 aattataggg ctatgattac ggttcgtgta agtgacgcca aaaaagaatg gctttcttat
      301 atggctgaat tttatgggat tactttatct gaacttctaa aaaactattc gatggaacaa
      361 ctagaagatg aatacaatcg tcaaacggct gaaattgccc ataaacattg gataagaagt
      421 gggaaaacaa cagtattcat ggaagaatcc ttaatgaatt tggtgggctg gaatgacctg
      481 ccgtttaaaa ttcacgcccg aaattcagaa gcagctctga ttggcaaata cgtgtattgg
      541 tagagataga agataatcaa ttcgttgtta tcgctattca agtagggcac cgaagaaatg
      601 tctatgatta agagactgtt tatttttaca aaaaaataat aattggaatc tagttaaaac
      661 tcttgaagtg agagatttga ctagattttt ttatgaataa aataaggaca aaattgttta
      721 ggaagaatca tgagtgtaaa aaaatcatcc aagaagtcga ttcttggatt ttttagtgga
      781 tttgttcgcc aaatacgttt agtatgcgta tatgagaggt tgtaagcaaa gcgttgtggt
      841 ctgagcactt aaggaggaat ctgtgaaaat agcttttcat atttttgcag attttgactt
      901 aatgccgaag gtcagctttg cgattaccgt ttatcgagag gctgtaaaag caccgtgtag
      961 ctccgatgac aataagcaag catttttaga gctaggtttc cttgattcta aatatgcggc
     1021 ttatttcaat gagttggtgc ttgaacgcga ttttaagaag gtcgaaaaca ggcacgactt
     1081 ttcgacgtaa ggagaaagaa tttcaatgat agaaacaata aatcccgcag aaaacaaggt
     1141 ttctgcgtac attcatatcc cattttgcga gcatatctgc tattactgtg actttaataa
     1201 agttttttta gaagggcagc cagtagatga gtatgtggaa atgctgctga atgaaatgag
     1261 attaatgctg gaaagtcatc ctacagcgaa tttggaaaca ctttatgtag gaggcggaac
     1321 accaacttct ttatctgcta aacaacttga tcgactatta tcaggtgcta gagagatcct
     1381 gccttttcaa aatggtaatg agtttactgt agaagcgaat cctggtgatt tgacccgtga
     1441 aaaacttcaa gtgatgaaaa actatggcgt caatcgttta tccatgggag tgcagacatt
     1501 tgacaatcgc ttattgaaaa aaattggacg taaacatact gcagaagatg tgtatcaaac
     1561 aatgcaattt ttagaagcag aaaattttac caatgtaagc atcgatttga tttatgcatt
     1621 accgggacaa acactagaag gatatcggga tactttggaa aaagctttgg ctttggatct
     1681 gcctcactat tctttgtact cattgatcct cgaaaacaaa acaatgttca tgaactgggt
     1741 acgacaagga agactcgaac tgcctgatca ggaaacggaa accagaatgt ttgaagagac
     1801 gattgaagca atggaaaagc atggacgtca ccaatatgaa ataagtaact ttgggctctc
     1861 cggacatgaa tctcaacata acctcatgta ctggaacaat gaccactatt ttgggtttgg
     1921 agctggtgct agcggttatc ttggagatat tcgttaccgc aacaaaggac caatccaaca
     1981 ctatttacgc ccattacgag caaatgaacg cccggtattg gaagaagaaa aattgacacg
     2041 aaaaaatcaa atagaagaag aaatgttctt aggactaaga aaaaaaacag ggattactaa
     2101 aaaacatttc tatgatcgct atcaaaaaac aattgaatcg gtgtacggtg atgtgctaag
     2161 ggaattagag caagaagggt tacttgttaa tgaagaagac cgtgtttatt tgacaccaag
     2221 agggactttt ttggggaaca atgtgtttga gcgcttttta tttgaaaaat agtttgaaga
     2281 atatttgttt tttatcgaaa cttgaaaaga aaaagtttag aaagtttgac aatgaggtat
     2341 tgaagtattg atataactgg atttacatca attatttaga attagcactc ttgtgtttag
     2401 agtgctaaaa aatggagtct ttctcttgac aggagaaggg cttcttgcta tatttataag
     2461 tgaattagca cttagaatat atgagtgcta atgagaggtg aggaagatgt taacaaagcg
     2521 acaaaacgac attttacgtc tgatcatcca acactatacg agtagtggcg tgcccgttgg
     2581 ttccaaaaca ttaatgtctg agggagtcaa ggccagctca gcaacgattc gcaatgatat
     2641 gaaagcatta gaagatgaag gattgctgtt gaagactcac tcttcttctg ggcggattcc
     2701 atctgcttta ggctatcgtt attacgtgga tcatttgtta aaacctgctc gcgtagcaga
     2761 agatgatttg ctgctgattc gtcagtctct aggcagagaa tttcatgaaa tcaatgagat
     2821 tattaagcaa tctgctgaga tattatctga gctgacaagc tatacgactt tttcattggg
     2881 acctgaaata aaagaccgcc gactgactgg ctttcgtatc gtacccttga ataatcgaca
     2941 gattattgct atcgtcgtaa cagatatggg gaatgtagaa agccaagtgt tcactcttcc
     3001 cgaatatatg ggaagtcagg aactagaaaa aatggtacag attgtaaacg accgtctagt
     3061 cggagatcct ctgatgacgg tgtaccataa acttcgtacg gagattccga tgattcttca
     3121 taaatatttc cagacgacag aagggatgtc tcagttattt gacaccgttc ttggtcatgt
     3181 ttttgaagaa aaggtttatg tcagtggaca aatgaatctc ttggattatg agccccgtca
     3241 agatcttgat cagttcaaat cgatgttttc ttttatgaaa gactcagacg gacttacaca
     3301 aatgatcact ccgatggata gcaatattca tatcaagatt ggtgctgaat taggcaatga
     3361 tttattgcag aatatgagta tgattcaagc aagttacgag atagatggtc atggaagagg
     3421 tacaatagcc ttgttagggc ctactagtat gccttattct cggatgttcg gtctagtcga
     3481 tgtttatcga agagaactag cagctaaact tgctgattac taccgctcgc ttgacttgtc
     3541 aaactcgtaa tgtgaataac gaagctattt gcagctgatc gttctctctt tcttagtaag
     3601 agcaatcaaa cagctgactg aataatgaaa ggaagactgt atcgtgaaag aaaatcaaga
     3661 agaactagat aaagaaatga cagatgctca gccagaaccg gaaatcgatg tcgaagctgc
     3721 agaagattcg ggtatttcag aagctgaagc ggaagaattc gaaacagcga aattaaaagc
     3781 tgagttagaa gaaatggaag ataaatactt gcgggctcgt gctgaaatcg caaatatggc
     3841 aaatcgagga aaaaatgagc gtgagcaatt acaaaaatac cgttcacaag atttagccaa
     3901 aaaattactt ccatcaattg acaacctgga acgtgcatta gccacagaag tgtcagatga
     3961 tcaaggggca ggtttgaaaa aaggcgtgga aatggtcttg gaaagtttgc gtaacgcact
     4021 ggaagaagaa ggaattgaaa aaatccctgc taaaggagaa gcctttgatc ctaatcttca
     4081 tcaagcagta caaactgttc cagcgactga agatacacca gccgatacga ttgtagaagt
     4141 actacaagaa ggctacaaat tacatgatcg cgtgttaaga ccaactatgg tcattgttgc
     4201 acaataattg tgcggatata aaacttacac tttcaattaa agaaggagac gttaattatg
     4261 agtaaaatta tcggtattga cttaggtacg actaactctg cagtagcagt attggaagga
     4321 aacgaagcaa aaatcatcgc taacccagaa ggaaaccgta caacaccttc tgttgtatca
     4381 ttcaaaaatg gagaaatcca agtaggggaa gttgcaaaac gtcaagctgt gacaaaccca
     4441 aatacgattt catcaatcaa acgccatatc ggtgaagcag gttacaaagt agatgtagaa
     4501 ggtaaatcat atacaccaca agaaatctca gcaatgatcc ttcaatatat caaaggattc
     4561 gcagaagact atttaggtga aaaagtcgaa aaagcagtta tcactgtgcc agcttacttt
     4621 aatgatgcac aacgtcaagc aacaaaagat gctggtaaga tcgctggtct agaagtagaa
     4681 cgtatcgtca atgaaccaac tgcagctgct cttgcctatg gtttagataa aacagatcgc
     4741 gacgaaaaaa tcttagtatt cgaccttggt ggcggtactt ttgacgtatc tatccttgaa
     4801 ctaggagacg gtgtctttga tgtattgtca acagctggcg acaaccatct aggtggggac
     4861 gactttgata acaaaatcat cgactacatg gtagcagaat tcaaaaaaga aaacagcatt
     4921 gacttgtctc aagacaaaat ggctttacaa cgcttgaaag acgctgctga aaaagcgaaa
     4981 aaagatttat ctggcgtatc aagcacacaa atcagcttgc catttatcac agcaggagaa
     5041 tctggaccat tgcatttgga aatgacattg acacgtgcaa aatttgatga attaacagct
     5101 gacttagtag aacgtacaaa agtaccagta cgtcaagcgt taaaagatgc aggtctgtca
     5161 caatcagaaa tcgacgaagt tattttagtc ggtggatcta ctcgtatccc tgcagttgta
     5221 gaagcagtac gtaaagaaac aggaaaagaa ccaaacaaat cagttaaccc tgatgaagta
     5281 gttgctatgg gtgctgctat ccaaggtggc gtgatcactg gtgatgtgaa agatgttgtt
     5341 ttacttgacg ttacaccatt gtcattaggt atcgaaacaa tgggtggagt gttcactaaa
     5401 ttgattgacc gcaatacaac gattccaaca agtaaatcac aagtattctc aactgcggca
     5461 gataatcaac cagcagtaga catccatgta ttacaaggtg aacgcccaat ggctgctgac
     5521 aacaaaactc taggaagatt ccaattgaca gatattccag cagctcctcg tggtattcca
     5581 caaatcgaag taacattcga tattgataaa aacgggattg taaatgtatc tgcgaaagat
     5641 ctaggtacac aaaaagaaca aaaaatcacg atcaaatctt cttcaggtct aacagacgaa
     5701 gaaatcgaac gtatggtgaa agatgcagaa gcaaacgctg aagcagataa agctcgtaaa
     5761 gaggaagttg acctacgtaa tgatgtagac gcattgctat tctctgtgga caaaacattg
     5821 aaagaattag aaggcaaagt ggacgaagaa gaagtgaaaa aagcagaaac agctcgtgac
     5881 gaattaaaag ccgctgttga agcaaacaac atcgaggaaa tgaaaactaa acgcgatgca
     5941 ttgaacgaaa tcgttcaaaa cttgactgtt aaactatacg aacaagctgc acaacaacaa
     6001 gctcaagaaa atccagaagc agctcaaggc ggagcagatg atgtcgttga tgctgatttt
     6061 gaagaagtcg atggcgacga taaataatca gttcactgaa tttcgagaga aggccaaagt
     6121 tgctactttg gctttttctt ttgaaaaata gtttgaagta tcggtttttg tttcaaacaa
     6181 aatatgatat gataacaagc gacgtgaaaa tgatgagaac caataggagg aaagcctatg
     6241 gcaacaaaac gtgattatta tgaagtctta gggctctcaa aaggagcatc agaagatgaa
     6301 ataaagaaag cttatcgtaa gctttcgaaa aaataccatc ctgacatcaa taaagaacct
     6361 gatgcggaag aaaaattcaa agaagtatca gaagcatatg agattttaag tgacccgcaa
     6421 aaacgcgctg catacgacca atacgggcat gcaggcacag atccaaatta cggtgctggt
     6481 ggagctggtg gcggatttgg tggattcggc ggcttctctg gtggcggctt cggtggtttt
     6541 gaagatatct tcgactcttt ctttggagga ggcggtcggt cagtcgatcc aaatgcgcct
     6601 cgtcaaggtg ctgatttgca atatacggtc gacttgtctt ttgaagaagc gatttttggt
     6661 gtagaaaaag aaatcaagta taatcgggaa gaaatctgtc atacttgtgg tggaaacggt
     6721 gcaaaaccag gcacacaacc aactacttgt cataaatgtc atggtgcagg tacgatcaat
     6781 gtagaacgcc aaacgccact tggacgagtg atgagccgtc aaacttgtga tgtttgtcat
     6841 ggcacaggga aagaaatcaa agaaccttgt ccaacctgtc atggctcagg acatgagaag
     6901 aaagcacatt cagttaaagt gaatgtacca gctggtgtag aagaaggaca acaaatgcgt
     6961 ttagctggtc aaggagaagc tggagaaaac ggcggaccat ttggtgattt gtatgtggtt
     7021 tttcgtgtag aagaaagtga tatctttgat cgtgaaggtt ctgaaattta ctatgaattg
     7081 cctttaagtt ttgtacaagc agctttgggg gatgaagtcc aagtaccaac tgtccatgga
     7141 gatgtgaaat tgaagatccc tgctggaaca cagacaggta cgaacttccg tttacgtgga
     7201 aaaggcgctc cgaagttacg tggtggtgca acaggcgacc aacatgtcaa agtaaaactg
     7261 atcacaccga aaaatctaaa tgaacaacaa agagaagcat tacgtgcatt cgctgaagct
     7321 ggcggcatca aagttgaaga acagcaagaa gacggtttct tcgataagtt taaagatgct
     7381 tttggcggga aaaagcggaa gtagtttttc tagtaaaaat ataaagtaga cttgcccaaa
     7441 ttttctattt ctagtaaaaa cgctaagtca ttaataatta aaaaccagac gaacaatcat
     7501 acgttcgcct gg
//
LOCUS       DASJTC010000110         7438 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.113, whole genome shotgun sequence.
ACCESSION   DASJTC010000110 DASJTC010000000
VERSION     DASJTC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.113"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="U4B19_002434"
     CDS             complement(52..1581)
                     /gene="ahpF"
                     /locus_tag="U4B19_002434"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEN2078124.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="U4B19_002435"
     CDS             complement(1591..2154)
                     /gene="ahpC"
                     /locus_tag="U4B19_002435"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEN2078125.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2298..2960)
                     /locus_tag="U4B19_002436"
     CDS             complement(2298..2960)
                     /locus_tag="U4B19_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2078126.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3144..4790
                     /locus_tag="U4B19_002437"
     CDS             3144..4790
                     /locus_tag="U4B19_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN2078127.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4781..5863
                     /gene="uxuA"
                     /locus_tag="U4B19_002438"
     CDS             4781..5863
                     /gene="uxuA"
                     /locus_tag="U4B19_002438"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN2078128.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5971..7438)
                     /locus_tag="U4B19_002439"
                     /pseudo
     CDS             complement(5971..7438)
                     /locus_tag="U4B19_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         2332 a         1364 c         1376 g         2366 t
ORIGIN      
        1 tcttgtcacc atcctttttt ccgctctctg actttttaca atcttcatag ctcaagtgcg
       61 catcagataa tcgaatgctc ctaaagctgc agtagctcct gaacccattg aaacaatgat
      121 ttgtttgtat ggactatctg tacaatcacc agctgcgaaa acgccgggga tattcgttgc
      181 gccatttttc tcggtcacta tttcatttct tggattcatt tcgatcgttc catctagcca
      241 atccgtatta gggagtaagc cgactagaat gaatactcct gaaacagcta gtgtatgcat
      301 ctccatcgta cggcgatcga tataggagag agattctacc tgattcgtac cgctgatttc
      361 tttcgtcgct gcatgtttga taacagtgac attcgcaaga gagactagtt tatcctgtaa
      421 gatttgatct gcttttaatt ctggtaagaa ttctagaaca tatacatgtt ttgctaaccc
      481 agccaaatcg attgctgctt caacaccaga attaccacca ccgataacag caatctcttt
      541 tcctttaaat aaagggccgt cacagtgtgg gcaataagca attcctttat ttttaaactc
      601 tttttcgcct ggtacattga tagaacgcca gtgcgcacct gtcgcgatga tggcggtttt
      661 tgctttaagt gatataccgt tagctaagcc aatattaata aaatgttgct ctttatttaa
      721 ggaaacggcc tgttgattca tcataatgtc gatagggtaa ctacgcacat gactttctac
      781 ttgctgcatc aattttggac cttctgtgta agaggtacca atcatatttt caatccctag
      841 tgtttcaacg acttgtccgc cgaaagaatc agcgacaagt cctgtacgga ttcctttacg
      901 tgctgcatag atagctgcac tactagctgc tggtccaccg ccaatcacta atacatcata
      961 aggttctttt tcaaacaact cttcttcaat caaagggcca gtgatttttt caacaatttg
     1021 ttcgatcgtc atgcgtccgc tactaaattc ttctccattt aaaaacacag ttggcactgc
     1081 catgattttt ttatcatcga tttctttttg gaacatccca ccttcaatca tcgtatgagt
     1141 aatatttgga tttaatacag ctaatatatt caatgcctgt acaacgtcgg ggcaattatg
     1201 acaagtcaag ctaacataac tgtcaaaatg caatgtttgt gttatttttt tgattttaag
     1261 actcaaagat tcactgattt tcggtgcccg tccactaatt tgtaacatag ccagtaaaaa
     1321 agaggaaaat tcatgaccga gggggatacc agcaaaaacg attccacttt ctttgccaac
     1381 tggattgata gaaaaactag gtgtgcgcat cgaatctctt ttttcccaag agattttagg
     1441 agacatttct gctatttcct tcgtaaattc agctagtttt tgactatttt cactattatc
     1501 caaactaaca gaaaaaacaa taggagattc aatcagtgct aaatattgtt cgagattttg
     1561 tttagtttct ttgtctaaca tatccaccac ctaaatcttt ccaacaagat caaaacttgg
     1621 tttcaatgtt tcgccacttt ctttccattt tgccggacaa acttcacctg ggtgggtacg
     1681 tacatattga gcagcgcgaa tcttatcaat cagtgcacta gcgtcacgac cgattccatc
     1741 tgcattgatt tccatagctt gaacgattcc gtctggatca ataataaatg ttcctcgctg
     1801 agctaatcct tgctcttcat ccaaaacatc aaaaatacga gaaatctgat gagacggatc
     1861 accgatcatc acgtattcga ttttaccgat agcatctgat gtatcatgcc atgctttgtg
     1921 agtaaagtgg gtatcggtag agcaagaaaa gacttcaaca cctaaagatt tcaatgtact
     1981 gtattgttct tgtaagtctt ctaattcagt aggacaaaca aaagtaaaat cagctggata
     2041 gaagcaaaga atactccagt tccccataag atctttttca ctgacttcta aaaattctcc
     2101 ttgatgataa gcatgtgcat taaattcttc gatttttttc ccaatcaatg acatagtaaa
     2161 acctccaata ataattattt atttataatt attgtaattt agttggagtt gcaaagtcaa
     2221 tggatatttt gttatttaat aaagattact caaaaacgaa ggttaattat aaaaatagaa
     2281 gaggtcgcaa gaaaatgcta ctgctggaaa tactcgggat aacgttgagt aacagtttcc
     2341 tgttctttca gcatcatgtg taagtgcaat cccgtttgaa atcgagcttc ttctggtttt
     2401 ttatcaacta aagccttgag taaatcataa tgttgttcca tgatcgtttc ccatggaagc
     2461 ccctgaatgg tcactcgtaa ccagcggaaa cgttctaaat gggtattatg gtcatctaac
     2521 cagttccaaa cttcttttct tcctgcaatt tcgaaacaag cttcatgaaa taaattatct
     2581 gcacggaaaa aagcgcgttc gtcatttatt ttgactgctt tttcttgttc ttctaaaatc
     2641 gtttctagca ctattcgtgt ttgtgtgtcc aatttggaac aacattcaat catgatttgc
     2701 tcttccaact gttctcggac aaagcgagca ttttctgcag aaacaagatt aattttggaa
     2761 acatacgtac cactttgagg tatcgtatac acgagttctt gctgacgtag ttgaatcaat
     2821 gcttcgcgaa tgggagtccg gccgatagca agatgttcct ccagtgcctt ttcagatatt
     2881 ttccttccag gttctaagtc agcatagata atttgtttac gaatcgcttg ataagcttgt
     2941 gattgcaagc tgcttttcat ctttattcct ccaaaatcat ttctatgaac gtagtataac
     3001 agaagaggtc tgaaaaacaa tctggtatgt cacattaact agtaaatttg tataaatagt
     3061 aaaattttgg ttgacatagc gatttttatt gcataaaatt cacttgaata caaaaactaa
     3121 tatgttagtt taggagggga aaaatgattg atttaaacaa ctggaggaac tttatccctg
     3181 aattatctca aaaagaagta gaaatacctc attttgaccg tgcatcatta agagaaaatg
     3241 gacaaaaaaa tcctgtctgg atccatttcg gtggcggaaa tctttatcga ggatttcatg
     3301 cagaaatcgc tcaacgttta gcggatctag gcgaattgtc cactggtgtc attgtttgtg
     3361 aaacattcga tgaagaaata attgaaaggg cttaccaaga ttttgatgat ttgattcttg
     3421 aagtggtcat gccaagcagt ggagagttcg tcaaacgtat attagctgcg acagctgaca
     3481 gtctgtattg tcatccagat tgttctgaac actttgataa gatgattcga cactttgaaa
     3541 atcctagttt acaattagtt acttttgcta ttactgaaaa aggttatcga ttgaaagata
     3601 caaataataa atatatagga ccgattgcag tcgatataaa aaatggtcca aagaagccat
     3661 cacatacaat gagtattctt gctgtgctac tttataaacg ttttttatct ggagcttggc
     3721 cgattgcact tgtctctaca gataattttt cacaaaacgg tagtcaatta cgtacttctt
     3781 tgctaacgat cgcaaaagaa tggcaaaagg aaggttatgt acaaaataca tttatccaat
     3841 acttatcgaa tgaagaaatt gttgcgtttc catggacgat gattgatcgt atcaccccga
     3901 atccttcctt gtcagttagc aaaatgttgg caaaagaggg cttttcaaat atgtcgattt
     3961 tccaaaccgc aaaaggaaat aatattgctc catttgtcaa tacagaggac atccattatt
     4021 taatcatgga agatcatttt cccaatggac gtccgaaatt cgaaaaagct ggggtattct
     4081 tgactgatcg tgcaacggta gataaagcag atacgatgaa ggtgacgact tgccttaatc
     4141 ctttgcatac gtctttagct attatgggtt gtttactggg gtataggagc atagctgagg
     4201 agatgaaaga tccagattta tcgctgctag tttctgaaat cgggtacaat gaaggcctcc
     4261 cagttgtgga gtctccaggt atcattgatc caaagatctt tctggaagaa gtaattactg
     4321 tgcgcttttc taatccaaat attcctaata tgcctcagcg aatcgccacg gatacctctc
     4381 aaaaggtggc gattcgttat ggtgagacga ttaaaaaata tatggaaata gaagttaaag
     4441 acccgcagac acttgtattc ataccattga ctctcgctgc ttggcttcga taccttattg
     4501 gcattgatga tcaaggaaaa ccattcatcc cgagtccaga cccactattg acagaccttc
     4561 agcataagct ttccgatatt caactgggta cgcaagatac atcacatatt catgagaaaa
     4621 tggctcctat tttgtcaaat aaagaaattt ttggtgtaga tctttaccaa gcagagcttg
     4681 gcagaaaaat agaagaaatg ttcatcaaga tggtagcagg accaaatgcc gtacgaaaaa
     4741 cattacagga ttacttacaa gaacatggag gaaatttatc atggaaatga cttttagatg
     4801 gtacggatca caggaagaaa agattacctt agatgaaatc agacagattc ctgcggtcaa
     4861 agggattgta ggcacattga tggatatacc agtaggagag gtatggccta gggagaggat
     4921 caaagaacta aaagaagaaa ttgagtctcg tgggttaaca ctaaaagtaa ttgaaagcgt
     4981 caatgtgcat gatgccatta aaattggatt accttcccgt gagcagtata tcgaaaacta
     5041 taagcagaca atccgaaact tggcagaata tggaatagaa gtcatctgct ataattttat
     5101 gcctgtattt gactgggtga aatctgacct tgactaccgc ttagaagacg gctcatccac
     5161 gttagctttt atatctgatg atattccaga tgatccaaga gaaatcgtcg cgcgcatcga
     5221 acattccagc aatgaatttg gattgccggg atgggaacct gagcgcttag ctcctatcaa
     5281 atccttgctt gaagcctatg catcagtaga cgaagaaaaa cttcgagaaa attttgccta
     5341 ttttctccaa tcaatcatcc caacatgtga agaggtaggg gtcaaaatgg ctgtacatcc
     5401 agatgatccg ccatactcga tttttggatt agctcgaatc gtaaagaatc gagaagattt
     5461 agattggttg tgtcgcatag tagacagccc ttataacgga atcactcttt gtacagggag
     5521 tattgcagaa gatccagaaa atgatgtata tgggatttta gcagaatttt gccaaagaga
     5581 tcgaatccca tttgcacatg ttcgaaatat taaattttta gctaatcgag atttctatga
     5641 ggcaccacat aaatccgaat acggttcatt agacatgtac cgcatactaa aaacaatgta
     5701 tgacaacgga tttaacggat acatccgtcc tgatcatgga agaatgatct ggaatgagac
     5761 aggaagacca ggatacggct tatatgatcg agcattaggt gctgtttatc taaatggatt
     5821 atgggaagca atcgaaaaag gaaacaaaag taaagataat tagtgaaaaa aactgtaatt
     5881 ttattatgta aataagaagg atgttcaaat tcttgataca taaatccgga ggttgttaat
     5941 aagcattcgg atttatgtat cttatttggt ctaggaagta acttgatttt cttttttcga
     6001 ttgatccatg agtgcttctt cttttaattc aaagatatat tctgagatcc tcataatcac
     6061 ttttttccag tcttctggtg tgatgattcc ttttaaaata tagatttttt tgatatgaga
     6121 aggcaattgt ttcaaagggc agtcgctgat aacgatgttg cagttctcca taaaatcatt
     6181 ttgtattttg atcgattcat ttgaaaaaat ttgttctatc ttaacgatca gatctttact
     6241 gatataaaaa tcacgcgtat agttcacacc aatcacaatt cgcggctgtt ttttgtagcg
     6301 atcatacaaa tgtactaggt cttcaaccat ccattgcaca ttttcctttt ctaaaataac
     6361 tggtaacaag tgttgatttt gttctctgaa atccatataa aattgttcaa tggcggtttg
     6421 gacttctttc aatacgctat gttcttgata aagcatgaaa gtaggaaggg gcgtatttgg
     6481 ttgattgaag ttaagaaatc cattataaag tttatttcgt gcgaatgcca atgagtaaag
     6541 aatcttttct ttttcaggta taaacaaatc aaatacagta caaagggact ccaccatttc
     6601 tcttgaataa agaacatgag gaacgtttgc tttttttaaa agtttgtatt gctccaagct
     6661 tgtttcttcc gattcgattt tcgtgataga aagtcgagct aaaatattca ctaagatgcg
     6721 ttgttcgcga caaattggca gattaggtcg caaaatatct acagagagat caataaaaaa
     6781 gtcaatatat tcatactctg tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa
     6841 attctcttta catattttta atagaaaata taatttatct atcataccgc tatttagtcg
     6901 gtcaagtact tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc
     6961 atcataaaat tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata
     7021 ttgaatttgt acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga
     7081 caaaccgtac tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc
     7141 actatagttt gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga
     7201 agtcaagatc tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca
     7261 gtaagccgga tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg
     7321 cagttctgtt agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa
     7381 atttgattca gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacga
//
LOCUS       DASJTC010000111         7412 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.114, whole genome shotgun sequence.
ACCESSION   DASJTC010000111 DASJTC010000000
VERSION     DASJTC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.114"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(755..1168)
                     /locus_tag="U4B19_002440"
     CDS             complement(755..1168)
                     /locus_tag="U4B19_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEN2078129.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1624..2109)
                     /locus_tag="U4B19_002441"
     CDS             complement(1624..2109)
                     /locus_tag="U4B19_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078130.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2248..2463)
                     /locus_tag="U4B19_002442"
     CDS             complement(2248..2463)
                     /locus_tag="U4B19_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEN2078131.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2687..3487
                     /locus_tag="U4B19_002443"
     CDS             2687..3487
                     /locus_tag="U4B19_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2078132.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3506..5374
                     /locus_tag="U4B19_002444"
     CDS             3506..5374
                     /locus_tag="U4B19_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEN2078133.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5367..5858
                     /locus_tag="U4B19_002445"
     CDS             5367..5858
                     /locus_tag="U4B19_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEN2078134.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            5846..6400
                     /gene="srlA"
                     /locus_tag="U4B19_002446"
     CDS             5846..6400
                     /gene="srlA"
                     /locus_tag="U4B19_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEN2078135.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6418..>7412
                     /locus_tag="U4B19_002447"
     CDS             6418..>7412
                     /locus_tag="U4B19_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEN2078136.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2628 a         1193 c         1415 g         2176 t
ORIGIN      
        1 cataagcccc aatgaatgac taaaaagaaa agcagaatca catgccatat catcccatat
       61 ttcttcaaga aaataaataa gcgggaaaaa gggaaggaat agtagaacat cgatccagca
      121 ccattttttt cttcttttga aataacataa aacaataaac atgaacgtgc agggaagtaa
      181 aaacaaagct ttactcataa ctaacaaaat accagctcct tttatttttt tctcccccac
      241 attcaaagag taacaatggt atgtacttaa taatagaaaa atggatcaac ttgtatgaat
      301 aacagctgaa aagatctgca ccaaataaaa aacttccagc caaaactgac tggaagttta
      361 tttttttctg aaatattcta acacaaatat gaaaaagatg gccgaagcta ataagccgat
      421 ttcgtagcaa aacaatgaga taccagagtc gtaataataa aatcctctaa tcttatggat
      481 aaaatagatc atcggacaag cagataagag taagtatttg ttaggccttt tgaataagta
      541 aaagaaagca ctggtcatta tgataaatgg tatccaaaac aaaagtttac ttaagacaat
      601 caaatgatca actcccatat tttttaccaa gagagatcat aacatcttta tcgtaaaaac
      661 aaaatatttt tttgctcatt ttgaaaattt aaacatactc gttcttatcc aaatatataa
      721 aaatcgcccc tcattcatcg agaggcgata agttctaatt taactcaatt ttttttgttt
      781 cttttggctg ataagctgtt ttaggtaata ccaatttcag cacaccgttt ttaaaggttc
      841 cagtgatttt ttcttcatca acattaggaa gatagaatga acgtttgtaa gagaccgaat
      901 gtctttcttt ccgtacatag ttgccatctt ctttatcttc tgtatgggac tcatgattgg
      961 cggagatcgt caaagtatta tctgaatatt caactgtcgt gtcttctttg tcacagccag
     1021 gcatatcagc agttagtttg tattcttttt cattttcgac taaatctact tttggataac
     1081 ttgaaacacc taaaaaatca ttgaatgctg gactaaacac gtcattgaaa tcagggaaca
     1141 tgtcgcgaat gctaggtaca tttgccattt ttgatcatcc tttcaataag tattttttat
     1201 ttatccctat ttacgtaata actctatttt gaaaaaaatg caagtataat ggttagcttt
     1261 gtgaatattt catctaatac gatacaaagg ctatctttct aatttgaaaa agggatcatt
     1321 aaaataataa gaagaaaata tgtcaaaagg taaaaaagta aattcttcta tccaaatata
     1381 gaaaaattga atctagaaaa ccgcccgaca ataaatttca ttaactcagt tgaccgaaaa
     1441 tgaagtagat accaaaaatt tatcaagaga gcaagtagag aagtgagtat attcataaga
     1501 gaaatagctg taacgaatta ttaggttaat gcaaatgctt atttatactc cgtataactt
     1561 tctcagaaaa aatgatacca ataatgactt gctagtactg taaatccgta taatatcatc
     1621 taatcaaaat ggtgttccat ttgcatctgt catcgtaatc gatccttcgg gtgacactcg
     1681 ataaaaacca acgcttccag atcctccttg ggaacggatt tgtttgctgg aagctgtgaa
     1741 caaataatct cgatcaattt tttgataaaa gctaataaca atatcgtcac tttgtaattc
     1801 ggaacggtgg gcaataagaa atagaagtgc tttgtcagag ttttctaatg gaagagaagg
     1861 tacagtgctt tcttgcattt cagatgttga agaaatgcta aacgactgaa tacttgattg
     1921 gctatgttgc gaatctacat taccagtaga agtattagta atggaagtag tagaagtact
     1981 tttagttgaa ccaacctcag ttgttgaaat ggctgctgaa gattctttaa cagaattctt
     2041 tgttttatat ccacaggcag aaaaaagtat tacagttccc aacaagcctg cgtaaatccc
     2101 tttatgcatg atagctcctc ctaaaataga ttatctttat tcagtatatc acttaaacaa
     2161 agaaaagcag atggtttaaa atgaaaaaac cttcccaata aagggaaggt tcatgaagat
     2221 aataactttt ttaataatta tagctttcta atatctttca tcaacttttc tagtaatcat
     2281 tgggaaaaat ttcaagcagt agatcgcaca aatacctacg acgatataaa tgatcttcgc
     2341 aaaaatagtt gttgaaccac cagcaatcat agctactaaa tcaagttcga acaagcctac
     2401 caacagccag ttcaaccctc ccacaatcaa taaagttaat gcaatagcat ctaaagtttt
     2461 cattagaatt ctcctttctt aaatgataaa acaattatat atcaaaaaga gaaacattgc
     2521 ttatataacg ctcaggaatt ttagcaatct atgcaaaaag atgaaaattt tttttgctct
     2581 tccattagta cacatttttt agctgaagct gtaaatttca tgtcttaatt ttttttagtg
     2641 agcttttata ctggatttgt aagaaaaaag aaggagagga tcacatatgt cggattggtt
     2701 ggatattgca ggaaaagtag taattgtaac tggaggttcc tcaggaattg gaagaagtat
     2761 cgtcgaaaat ttgttgaagc agaatgcaca agtagcgaat tttgatgtaa ctgaatgccg
     2821 tatacagcat gaaaacctac tatctttaaa agtcgatgtg tcttctaaaa cggacataga
     2881 agaagggata tataaagtaa tgaaacattt caagacgatc gatggtctag tcaataatgc
     2941 aggaataaat attccttctc ttctaataga tagaaatcat ccaaaaagca agtatgaatt
     3001 atcagaacaa gtttttgata agatgatcgc tgtcaatcaa aaaagtgtat atttgatgag
     3061 ccaagcagtt ggaagaatct tggtgcaaaa gggatcagga gtgatcgtga atctttcttc
     3121 tgaatcagga ttggaaggat cagaaggaca gagctgttat gctgctacaa aagcagcgat
     3181 gaacagtttt actcgttctt gggcaaaaga gttaggaaag aggaatgttc gtgtggtagg
     3241 tgttgcacca gggatactgg aagaaaccgg tttgagaaca caagaatatg aagaagctct
     3301 ttcttatacg agaggtataa gcgtcgaaca gctgagaaac ggttacagtc gaacaagtac
     3361 gatccctcta ggaaggagtg gtaagctgca agaagtggcg gatctagtct gctattatct
     3421 ttcagaaaga tcaagttata tcactggagt cacgactaat atttcaggag gtaaaacaag
     3481 aggataaagg atagggggga cgcacgtgac gctagtaaac agatggtacc aattattgaa
     3541 attactagtc actcacaaga aagtttcaat ccaggaagtg caaaaagaaa tcaaaaccag
     3601 taatcaaacc ataaaaaaag atattgggga gttgaatgaa gaaattgccg gtattgcaaa
     3661 aatcatccaa aaaaacaaac attttgaact tgaaatttat gattttgatt tttttgatga
     3721 gatcatgcaa ggaaaattga aaacagccag tgatttcaat tcggcatcca aaagaatcgc
     3781 ttatttacta aataggctga tcgaaacaga ggatttctta cgtatggatg atttgtctga
     3841 agaagtcggt gtcagcagag gaacagtggt caaggatcta aagacattaa aagagatgat
     3901 caatgatttt gatgtaaaga ttacaggcac accaaataaa ggactaagaa taataggaga
     3961 tgagctagaa ttacgactac ttttatttta ttttgtttct ccctactttt cagagacgaa
     4021 aacagaacag tttctagaag aaaaacaagc aatcaaacat tttcagaaga agacgaatgc
     4081 tagtaaacaa gaaatcaccc ttttaacgaa agttatcggt atttctttga atagaatctc
     4141 tagtaactat ctgttgaaga agcccatcaa tcgttacagt gggtgttttg aatatatcca
     4201 agaattcaat gaactgatca atcagttgga agaaatctat gaactaactt tgagccaata
     4261 tgaaaaagat tttatcagtt ttcctttgaa catcatcaac acaagtataa cgatgataag
     4321 gaaagctaac tctgatttgc gcttatattt cgaccccatg atgatgcgta ttcgacgatt
     4381 agatctcatt gatctggatg agtccttctt atatcaagag atgaaacagc acctctcttt
     4441 attgataaat cgggtaattt ttcgttatcg attgactgat ttattttatg gtgagataga
     4501 aaaaaagtat cctttttctt accaaatcgc tagcgaatgc atacaagcat tagaagctat
     4561 cttgcaacat aagatctcta aagtagagat cagctaccta acgttgtatt tggagttagc
     4621 atttcgatat caaaaagagc atttttcctc taaaaaagta gccattgttc atacgacagg
     4681 aaaaggaaca gcattaatga tccaaagaca aatcaaaagg atattgggga gggatataca
     4741 aataacacat gtttcagaag aacaatatga aaaagtagat ctaaacaaat atttcgctgt
     4801 ttttacaacg attccgttaa aaaatgtaaa tgaagagaca ccggtgatcc atttgactaa
     4861 tctttttgat gactcttggt taagggcaca atggcaaaaa gcaaaaaaaa agcgattagc
     4921 cgggtttcat tctactgttt ttttctatca aatatggaaa gaaaaaaaga cgtatcaaga
     4981 atatttaaac aaattagtcg ctcctttgat cagaggctat tatgtggatg aagaatttga
     5041 aaaaaggatc ttgaaaagag aatatcagca aagcacattt gttaacaaag aatttgcttt
     5101 tcctcacaca accaaccagc tatctcaaca cattgtttta tcagtagggc taataactga
     5161 tcaaggttta gcagaaacag gaggaaatgg agttattttt ttatttgcca tacctgaaga
     5221 aatgacggct gaagttgaga atgaattatt tgagctatac gatatcctat ttgaactcat
     5281 aaaaatggga atagatgagt ttattgaatt agcaccgaca caagaatcat ttttaaaact
     5341 aatcaaaagt aaaggagaga gacttcatga atgatatgta tctattcata gttttcgcag
     5401 cttcagctta tgtgctgcaa gcagttatca gttggaagca actaaagcat ttcaatcaaa
     5461 catatagcga actgcgaaga aaaggaaaag tagcgattgg taaacgacct gggaaaatcc
     5521 gttcaggcac gattgttatg ctagctatag acgatcatga tcaagtttta gaagtcagaa
     5581 aaatgcaagg ggttacatct ttggcaaaat ttcattcatt acctgtctat gcgggtgaag
     5641 atatccatta catcgatcgt taccatccct cagttcgagc ggaagatcgt ttgacacaag
     5701 cagctttaga aaatgccaga gaagtatact tacgaataga ggcaggaaat gaaaaaagtg
     5761 aacaaccttc attgcctttt attgctatgc tcaataattt tcggatatgg aaaagtacga
     5821 tcaaagaaaa aaggagtgac aaacgatgga tatcataaca aaatttgctg agggatttat
     5881 gaggctgttc caaacaggtg cagaaacatt cataagctgg atgacaggaa tcgtaccagt
     5941 tgtcttgatg ctgatggttg caatgaatac gctgattgct tttttaggtg aagaccgagt
     6001 aacgaaagta gccaaattat cagcgaaaaa tccgcttact cgttaccttg tgttaccgtt
     6061 cttatctgct tttatgttgg gaaatcctat gtcatttaca atggctcgtt tccttccaga
     6121 atactacaaa ccaagctatt atgctgctca agctcaattt tgtcatacga gcaatggtgt
     6181 attcccccat attaatcctg gagaattgtt tgtatggatg gggattgctc aagggatcga
     6241 aaccttaggg ctaaattcga tggagctggc tatccgatac ttgcttgtgg gactgctgat
     6301 gaactttatt ggtggttgga taactgattt tactactgga tttgtatgca gacagcaagg
     6361 aatcgttctt agcaaaaaag tagagctttc agttgattag agaaaggaga gttttaaatg
     6421 tatggtgata ttaagatcgt aagaggaaac ggcgggtatg gaggtccttt gattattagg
     6481 ccgacaaaag ataaacacaa gttcatctat gtcacaggtg gaggtgagag gccaatgatc
     6541 gtagataaaa tagaagaact gacaggtatg gagtctatca atgggttcaa aacatcaata
     6601 cctgatagtg aaatcgcgct tgcaatcgtt gactgtggag ggactcttag atgtggaatt
     6661 tatcctaaaa aagggatacc aaccatcaat atcgtttcca caggaaaaag tggcccatta
     6721 gcacaatata tcactgaaga tatttatgtt tcagcagtag ggttagaaca aatacagcca
     6781 ctaatggaaa ctgatgataa caaagaaaca gtaaatactg catctccaga taaaaagtac
     6841 aaatatgata cttctaaaaa gataaccgag caaaaagcag agcagcaaaa ctggatagca
     6901 aaaattggaa tgggcgcggg aaaagtagtt gctacattca atcaatcagc aagagaagca
     6961 gtagatacga tgatccggac agtgatccct tttatggctt tcgtttccct tttgattggt
     7021 gtgatacaag gttctgggat cggtaatgta tttgctaaca ttatggcacc tttagcagga
     7081 aatgcagttg gattaacaat cattggcttt atttgttcac tgccattttt atctcctttg
     7141 cttggtccag gggcagttat tagccaagtg attggaacat tgatcggtgt agagattggg
     7201 aaaggaaata ttcctcccca attagcatta ccggcactat ttgctatcaa tactcaaaat
     7261 gcatgtgatt ttattccagt cggtctaggt ttggaagaag caaaagctga gacagtagaa
     7321 gtaggtgtac catcagttct ttattcaaga tttcttaacg gcatcccacg agttatcatt
     7381 gcttgggtag caagttttgg attatatagc tg
//
LOCUS       DASJTC010000112         7141 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.115, whole genome shotgun sequence.
ACCESSION   DASJTC010000112 DASJTC010000000
VERSION     DASJTC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.115"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..615
                     /locus_tag="U4B19_002448"
     CDS             <1..615
                     /locus_tag="U4B19_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN2078137.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="U4B19_002449"
     CDS             612..1385
                     /locus_tag="U4B19_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEN2078138.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="U4B19_002450"
     CDS             1382..1780
                     /locus_tag="U4B19_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEN2078139.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="U4B19_002451"
     CDS             1965..2636
                     /locus_tag="U4B19_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078140.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="U4B19_002452"
     CDS             2695..3135
                     /locus_tag="U4B19_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078141.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="U4B19_002453"
     CDS             3147..3815
                     /locus_tag="U4B19_002453"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEN2078142.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="U4B19_002454"
     CDS             3879..4502
                     /locus_tag="U4B19_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEN2078143.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="U4B19_002455"
     CDS             4558..4944
                     /locus_tag="U4B19_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEN2078144.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="U4B19_002456"
     CDS             5157..6548
                     /locus_tag="U4B19_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEN2078145.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         2277 a         1160 c         1572 g         2132 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc
     6781 ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga
     6841 gcaatttcaa aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt
     6901 gtcaggagaa caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt
     6961 atttattcca tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa
     7021 caaccaactt atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc
     7081 tttcgtagat tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga
     7141 t
//
LOCUS       DASJTC010000113         6978 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.116, whole genome shotgun sequence.
ACCESSION   DASJTC010000113 DASJTC010000000
VERSION     DASJTC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.116"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            290..886
                     /locus_tag="U4B19_002457"
     CDS             290..886
                     /locus_tag="U4B19_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2078146.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            complement(944..1426)
                     /locus_tag="U4B19_002458"
     CDS             complement(944..1426)
                     /locus_tag="U4B19_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078147.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1532..1729)
                     /locus_tag="U4B19_002459"
     CDS             complement(1532..1729)
                     /locus_tag="U4B19_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078148.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            2024..2782
                     /locus_tag="U4B19_002460"
     CDS             2024..2782
                     /locus_tag="U4B19_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEN2078149.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2842..3270
                     /locus_tag="U4B19_002461"
     CDS             2842..3270
                     /locus_tag="U4B19_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078150.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3316..3388
                     /locus_tag="U4B19_002462"
     tRNA            3316..3388
                     /locus_tag="U4B19_002462"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3349..3351,aa:Gln,seq:ctg)
     gene            complement(3538..3909)
                     /locus_tag="U4B19_002463"
     CDS             complement(3538..3909)
                     /locus_tag="U4B19_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEN2078151.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4124..4309
                     /locus_tag="U4B19_002464"
     CDS             4124..4309
                     /locus_tag="U4B19_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078152.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4392..4580)
                     /locus_tag="U4B19_002465"
     CDS             complement(4392..4580)
                     /locus_tag="U4B19_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078153.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4604..4867)
                     /locus_tag="U4B19_002466"
     CDS             complement(4604..4867)
                     /locus_tag="U4B19_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078154.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            5025..5282
                     /locus_tag="U4B19_002467"
     CDS             5025..5282
                     /locus_tag="U4B19_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEN2078155.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5353..5544)
                     /locus_tag="U4B19_002468"
     CDS             complement(5353..5544)
                     /locus_tag="U4B19_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078156.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5685..6005)
                     /locus_tag="U4B19_002469"
     CDS             complement(5685..6005)
                     /locus_tag="U4B19_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078157.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(6028..6462)
                     /locus_tag="U4B19_002470"
     CDS             complement(6028..6462)
                     /locus_tag="U4B19_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEN2078158.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6797..>6978
                     /locus_tag="U4B19_002471"
     CDS             6797..>6978
                     /locus_tag="U4B19_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEN2078159.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2186 a         1361 c         1069 g         2362 t
ORIGIN      
        1 ctataaaagc aagtaatttt taatggcaat atcataaaaa aaagaagata gagatttaat
       61 aattatatat cagctgtttt tttcaaataa gcaaaaattc ctaaaacaca acaatgaaag
      121 cgataactaa gaaaatcctt acataacgat ttttcttatt aaaattgttt tttgaacttc
      181 gcttattttt tgtgagcata tctgtttact tactttttgt aaggtgctat acttcatttc
      241 gtaatcaatt aaaaaaatgc aaaacgcaaa tcaaaggaga aattttatta tgacaacttt
      301 tactgacact ttgaaaaacc gccgttcaat ctatcatcta ggacgtaatg tttcactttc
      361 taatgaagaa ttaacaactt tgatcaaaga agcaattaaa gaaagcccaa ctgctttcaa
      421 tgctcaatca acacgcgcag ttatcctttt cggagatgca catgaaaaac tttgggaaat
      481 cactgaagaa gcacttcgcc cattgactcc agcagaagct ttcccaaata cacaaaacaa
      541 attagctggt ttcaaaaatg gttatggtac agtattattc ttcaaagata ctgacgtggt
      601 aaaaggtcta caagaacaat ttgaattata tgcagacaac ttcccagatt ggtcagaaca
      661 atcaaacgga atcgcaacag ctaatacttg ggtagcatta gttgataaag gattgggcgc
      721 aaacttgcaa cattacaacc cagttattga cgaagcagtt gcaaaagaat ggaatatccc
      781 ttcaaactgg aaactacgtt cgcaattagt attcggttca ccagaaacac cggctgagga
      841 aaaagaatac atgaacgatg ccgatcgctt ccgcgtattt ggataatttt atttgaaata
      901 aaaaacagag gttgtgacaa acatcttgtc gcaacctctg tttttattct ggatcaatca
      961 taatatcaat ctctgcctca tcgtttaagc gttgggacaa aaatggaact aattcttcta
     1021 atgatgcaga aaatttcagc atcggtcctc gccatccgtt tcggaaaaaa ttcattttta
     1081 cgattttaga accttttggc tgatataata caattttatt gatctcagaa aaaggaacac
     1141 cttttttgtc aaagctattc aaaacaagtc cttcttctgt gattcctcta ctgtccaaca
     1201 aaaaactaat aaaaatcatt gctgacaaga tcccttttgc caattcatct gctgtaggat
     1261 gactgatgag tgtataccaa atgactagtg acactaagaa gggtatcacc aagtagaccc
     1321 atcgatttcg cacagtccga taaataattt cttttcgaac cagccaaaga aataaaaaca
     1381 tcacaacaac gaataaaaac aatagaaccg taaagaatgt taccatgaca actcgcctcg
     1441 ctttttcttc tccagaaaat aggaaaagaa tactgattta ccttctcaaa gaaattggac
     1501 ttgcagcacg ccgcacgtcc aattgttttc tttattcaaa tgtatcatat tttgcaacaa
     1561 ccatgcttgc tttttcatct aataattctt ggtcctctgc agcacattgg gcaactttga
     1621 ccacggcgca gtttgtcatt tttgatacaa cttcaccaat gaccgttttt gtaaaaccaa
     1681 ttggtttgca tgcatattgt tctcctaaat taatcatttc ttccgtcatc aaaatcctcc
     1741 taaactgctg gaacttttct ccacattacc actgctattt ctttggagat ttaactcatt
     1801 gatttttatt tacagttaaa aatcaacctt acaggcatta tagcgtactg ttttcgtttt
     1861 cacaattaaa acagctgttg tatttagaag aatttttttc aaaaaattaa aacatattcc
     1921 ttaaaaacta attatttcct acatattaaa atataatact tccatttcca ataaaaatac
     1981 atttataata ggaaagataa acagctgaaa agggagattt caaatgaaga aaaaaacgtt
     2041 caaaaaacta ttgtttctag ggggcacagc tgctctttta ttaagcggta caagttatta
     2101 cctacatatc cattctccag aacatacaat gaacactgca caaaaaagtt caacacaaaa
     2161 aacgaaaaag acaaaagaga aagcagaagt tttactagat gttccattag agagccagtt
     2221 tgattctcct tctttagaaa atggctgtga agtcacttcg ctttctatgc ttttatcttt
     2281 ttatggttat gaaacaacta aaaaccagtt agctgagcaa ttggattacg tccctgtttt
     2341 caatgcagat gggactcatg gcgatcctaa tgaaggattt gttggtgata tcagtggcgg
     2401 agactgggct atggtcgtct atgttccccc agttgcctcc cttgctcagc aaatcgtcca
     2461 aacggattat cacacttttc caaaaaccga tgctcaactc gatgatatca aaaaagcact
     2521 atctaaagga aatcctgtct ggacttctgt gactatcaat tttgaagtcc cagatgaaac
     2581 tgattttatg acttggacca caaataatgg agaagtaaga gtcactcctc ttgttcatgc
     2641 ttgtgtagtc actggatatg acaaagaaaa tatttatgtc aacgatccct atggtgtaaa
     2701 aaatcgagca gtaccgatca acgatttttc ggcgattttc actgcaatgg gtggacagat
     2761 gctaactttg gagaaaagct agtaattcaa acaaaaatcc cttatactca agataccaaa
     2821 cctttgaagg gagcgtagat aatggaaaaa ttcacacata aaaaaatgga tccaaatgaa
     2881 attccgatta tttttgtccg tgactgcaag ggaaatgttc aaggtaaagt ctctattaat
     2941 gagtggaacg agcgtcgcag acctgctacc ctcaatgaac tggaaatcaa attgtatcgt
     3001 caatctcttg tttattacgc agatcaagag tacgagaaag ccaccgattt attgaaattt
     3061 ctaattgctc gaacagagta tactcgcttt gaatatattg aacgtttagc aaacatctat
     3121 catattatga atgaacctgt caaagaatat cagttattgg acaccgtctt gtcagtagca
     3181 gaactgattg cccttcctgc tggattagaa aagaaactag tacgccgcct tttacgagta
     3241 aagcaacaac tatccgatca agaaaaataa cttgcgtttt tttaggaaag tgctatgatt
     3301 attttgttga ataaatggga tgtagccaaa ctggtaaggc acttgactct gaatcaagca
     3361 attgtaggtt cgaaccctgc catcccaatt tgatgtgctt gctgaaaaaa tgaaccccct
     3421 gtaaaagaac aatctttttg cagggggatt attgtattat ttttactact tgtatttagt
     3481 ttacataatt aaattatgaa taataaaatc gggcgtattc ttaatacaaa aactgattta
     3541 cccaataatt gctaatgctt tctccggaac tttttctcta ggaacttcct catacgtttc
     3601 tatgtgtgct cctttattgc taattttcaa ataatgattt tcttttaaca cagaaagacc
     3661 attaaattca accgttcgtt ctttcccatt ggcatcagca gctttctgca cataccttgc
     3721 tgtaccatat tcatttactt cctctggttt ttgtgtcttt acatagacct ctcctttttc
     3781 aactaatgga tttagctgat caagtatacc agggatttct ccataacttc cttcagtata
     3841 atatttcagt ccaaatagca aaataccact aatcgccgcc agtccaatca aatatttaat
     3901 cactttcatc tttctacctc cttgttatat attcagtata tgggtcagtt aaagaaattc
     3961 catccgtccc aagttggaga tttttctcct actttagaag cgagaatcga gtgttttttt
     4021 agtaaaaatg atcgttattt gatattattg acgtgtggat aaatcagttt ttctcgtttt
     4081 gtccatgtaa aaaatcatgt tgatagcaaa ggatgtgttc aaaatgaatc aagaagaatt
     4141 atttcaaaaa gtcaaggaaa tgattaagaa cggcaatttc gatggcgcaa aacgatttat
     4201 cgaagaacat aaagaacaat taggacctta taaagagaaa gcccaaaatc ttttaaaaga
     4261 tgtaaatatc gacagtgtca aaaacaaatt taaaaatcta ttcaaataag taaaactttc
     4321 acttttgtaa aatcaaagga ggacaatgct gctgcatcgt cctcctttgg ttcttttctg
     4381 aatacttcat actaaaataa acgtgtcgga tcgtaatgat aagtcacatt gccgttcagt
     4441 gaggcttttc ccaccacata tttacgattc tccaacttca gccacgcttc tcgaaaagcc
     4501 attaattctt ctctttttac atcaatcttt tcgatttttc catcgatcaa atcttgaata
     4561 agttgttcat atggattcat ttttttcctc catctgctgt caatcaataa gttgtaaaaa
     4621 taggttcata tgcttttttg gtatctagat cttctctgac tttgactgtc gtataaataa
     4681 ttttttctac tgggatacta taataacttc cttcaccatt attgaatcgg tgaacgtctt
     4741 tctttgctaa aataccattt attttattcg tcagtacacg ttgttcgaca ctgttactta
     4801 caacatagta aatcacccga atattattga tcaaatgcaa atcgatttct aacactacat
     4861 gatccatgaa aatcccactc cttctttcac tttgattata gcaagtttct cctctaattt
     4921 caaaatcgat acagatttta ccggataaat ccacaaaact gcttgcgcca tttcgcacaa
     4981 agtgttattt tgtatcatgg actttattta tcggaggcaa tagtatgatc gcgatcggtc
     5041 aatttgtttt ttatattcct ttttttatta tgataagcat tcttttctat tacataaaat
     5101 ggacaaaaaa gaagttttca gttttactgg cctctttacc tgctgtatat tttacttatc
     5161 aaattttttc ttttaggcat tgggaaacaa cgtctgtatt aataacacac atcatcgaat
     5221 tgacgctttc agtaattttt cttattattt ggatctattt tttatataag aatcagaatt
     5281 gaatccctcg catgtttaaa aagaaaacga ttaaagatgc agcaaatttc tgccgatctt
     5341 taatcgtttt ttctattctg ttataccaaa aaaatgtttg aatcggtgaa ggatcgttcc
     5401 ttgttttaat tctataaatt cttgtgcgat cgggtacttt tcttccaaat tttctttgtt
     5461 cttttcgatc aggcttttcg cttcttcata ttttttatct gcacatagtg cttttacttc
     5521 ctgtaatact tttcctgctt ccatttgcct caccctcttg ccattttatt gctttaagtt
     5581 ttatttaagt tatacgcatt attctagttt catcccaaat aacctttttt catttttact
     5641 cctcgaacta atcagttttc taaaggctga ttggttcgtc ttttttattt ttcctccatt
     5701 tcattctgct cttctgttaa tttatctaat gctttgattg atgctgcggc tcttgcataa
     5761 tcatagctgt ataattccat aaatttatca ggcagaacaa accacgctgg atgataaccg
     5821 ttagctgtga cagggaatcc tttatcttgt tggttatctc catcccatct cagagcaaca
     5881 cctttttttc ctttccactt tactaaagcg atcgagtatc ccttctttgt ttgtgtgccg
     5941 tcttcaataa ttgctaaaat ttctattttt tcttttggcg cacggacttc ttgaggacga
     6001 accattttct attcctccct tatctcatta cttcacgacc aacacactgc aaggggcatg
     6061 tgatacgaca taagaagcgg tcgatccaac taatgcttgt tgtatagcac cttttcctgt
     6121 cgctcccata acgatcaaat caatattttc ttgtccaggg atgacattgg caatcaaatt
     6181 tcttggatct ccagtttcaa ctattgcgtg gatctcagca atgccgtact ctttggcatc
     6241 attgattttt ttcagcatgg ctacttctgt tctttgtttt tcatctgcat agatttttga
     6301 aaaggcatag gcactatggg tcagctcaac tttgttgatg attgacacga taaataagtg
     6361 ggcttcattg tcctttgcta agtcaagggc ttcagcgaac gctttgatcg attgtcttga
     6421 tccgtcaact gctaccatga tgttctggta tcttttactc attggaaccg cctccttttt
     6481 cttttattgt aacatctcaa gcaaaaatac aaaaaatata cgcaatatgt aaataaaatg
     6541 atgaacaaaa gcaatttctt tcaagttatt tatacatttc ttttctcatc agaacacctg
     6601 ttctaatccc attatttctg cattatgtga aattcctcat catttttcac atcgagcgaa
     6661 catcattctg ttacaaaaaa cgtggatttg tcataaattg tcatgcttct tttacctatg
     6721 aaatattttc acgtgttaaa gtattcctcg ttgaacacaa caaataaaac tttttaacga
     6781 ataaaggaga tttttcatga catcacttaa aactttactt tttggaacaa ctttggctgc
     6841 cggcgctgca ttcttcatgg gaacgactgc tcatgcggac gaagcttata ccgttcaatc
     6901 aggtgatact ctatcaacga tttctcaaaa atacgttggt gataactcct tgatcaatgc
     6961 cattgcagaa tcaaattc
//
LOCUS       DASJTC010000114         6954 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.117, whole genome shotgun sequence.
ACCESSION   DASJTC010000114 DASJTC010000000
VERSION     DASJTC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.117"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..871)
                     /locus_tag="U4B19_002472"
     CDS             complement(<1..871)
                     /locus_tag="U4B19_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2078160.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVV"
     gene            complement(991..1638)
                     /locus_tag="U4B19_002473"
     CDS             complement(991..1638)
                     /locus_tag="U4B19_002473"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HEN2078161.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1722..3149)
                     /gene="uxaC"
                     /locus_tag="U4B19_002474"
     CDS             complement(1722..3149)
                     /gene="uxaC"
                     /locus_tag="U4B19_002474"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEN2078162.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(3223..4302)
                     /gene="uxuA"
                     /locus_tag="U4B19_002475"
     CDS             complement(3223..4302)
                     /gene="uxuA"
                     /locus_tag="U4B19_002475"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEN2078163.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(4305..5936)
                     /locus_tag="U4B19_002476"
     CDS             complement(4305..5936)
                     /locus_tag="U4B19_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEN2078164.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            6240..6911
                     /locus_tag="U4B19_002477"
     CDS             6240..6911
                     /locus_tag="U4B19_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2078165.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         2263 a         1384 c          951 g         2356 t
ORIGIN      
        1 aaacaactat taaaaccaaa ataggattga taaaattcat taaaactatt ccctgtgcat
       61 cattatagta atcaggaaat acataattat tcatactcat aattaataat tgagcaagga
      121 gtaagaaaat agccgcacca attatagaaa gtaatgccct atttttaact aaccctttta
      181 aagattttaa caatgaaact ttgtattgag attggtcaat tcttactcgt tcagtagtta
      241 gtttataaca tattaaataa caaataagtg cacatatcga aaatattccc gcaacaattg
      301 taaaagttga tccaccacga actatttgag cgcctgaatc atctcttgta taaataaata
      361 atggtgttaa tacaccaatg accaaacttg ctaaagtagc tccaacattt cggaatgtcg
      421 atagcattgt tctttcttct gtaatagaag taatagctga tgccattgat ccataaggaa
      481 tatttataga tgtgtaaaaa atactccccc ataatatata cgtaacatac atgtatacta
      541 ccttaacgct catagaagct gaaatcatag aagtttgata cattaaaaaa cttgctaaag
      601 cgactggacc actcattctt aaaatccacg gtttaaactt accctgctta gttgctttcg
      661 atctatcaac aatacgcccc atagttacat cagtaactgc atcgataaat cttgctacta
      721 aaaataatgt tccaacggca gctccgctta tccccaatac ttttgtataa aagatcatta
      781 aaaaagaact agcaaaaatg aaagtaaaat cgtttccaaa atctccaaac atatatccta
      841 ttttgtctct tataccaaat tttctttcca tacaattctc tcccttctgt tgttaagcgt
      901 tttcattctg atgaaagtac gtactatact ctgaagcgta aagagtcagt atttattttt
      961 tacatttcag agtattattt gttttttctc ctaataattc tttatctcat aaaacttatc
     1021 tatatattta ctagctactc gacttacttc ttcaaaatca cctttttcaa cagaagccaa
     1081 taaattccca cctacaccaa ctgcaacaac tcctacttca aaccaatctt ctaagttctc
     1141 tatacttact ccgccagtag gcattatatt tacgtgtggt aatggagctt gataggcttt
     1201 tacaatggat ggaccaaaag cagaacctgg gaatagttta atgatatcta cgccagcagt
     1261 aagtgcctcc tttatctcag taattgtcat acaacctggt aaataaggaa tttgatataa
     1321 attacaaatc tcagctgttt gtcgatcaaa agatggacta acgatatatt ctgccccagc
     1381 cataattgcg atacgagcag taaacgcatc taaaaccgta ccggctccga ttattacatc
     1441 tttttgatca gcatattttt tagcaatttc tttgattatt tcttcagcat taggaactgt
     1501 aaaagttagt tcaatccctt taattccacc cgcaataatt gaatttattg ctttaatggc
     1561 ctcatcctta tttttcccac gtacaaccgc aatgactcca gccgcttcta acttacttaa
     1621 aatttctact tttttcatat ctatctcctt cctttatgga tacatacttt gtctagctgt
     1681 actaacttac aaagaataaa tttaagcttt tatttcaagt attattttct acttccgatt
     1741 tcaaaaaaat taaaataatt atacgcatta cgataacaaa tattctccac tatctgacta
     1801 agatacattt catcatctgg aattcggcct gctttaaccc actcgcttag taattgacat
     1861 aatactcgac gaaaatactc atggcgagga tagcttagaa aacttcgaga atcagtcaac
     1921 atacctgtaa aattcgacaa caaactttgt tgtgccatcg tagtcaactg ttgacgcatt
     1981 ccttcagctg tatcattgaa ccaccaagca catcccaatt ggattttttg aatcataccc
     2041 ccttggaagc tttgcattgc tgttgctagt ggaagccaat cagttggatt taatgaatat
     2101 aaaattgttt ttggtaagca cccactcttt tcagcggtat cgaacaatgc tacaacattt
     2161 tgtgtcaaac tagcttgatc ccccatcgaa tcaccaccga catctactcc aatttgttcc
     2221 tttaatatac ttgaattatt tcttaaagca tttaaatgca tttgcatcgt ccaaccatat
     2281 cttgtatact cagacatcaa atgtaattga atacctgtaa caaattgtgc aatttctgat
     2341 aaatggaaat ccgtttttcc tgctaaagct tttattaaca gattgtcgag tattttttca
     2401 tctgctattt cataaaaata agtattcaaa ccgtgatctg ctaatcgaca gccaatttga
     2461 tgaaaatgat ccactcgttg gctcaatgct tgcttcaacc cattaaagtc tttgattggt
     2521 atagccgctg tttgactaag ttttaaaata tagttcgaaa agcctgatga tttgatactc
     2581 cagaccttat ctggacggaa agtaggtaat accttgaaag aattatcctg tttagaaagc
     2641 tcagtatgat accacaagtc atctgtagga tcatccgttg tacagatggc tttcacatcc
     2701 atcatctcca acaattcttt cgggcgatag gaatccgttt gaattttttc gttagcacgt
     2761 cgccaaattt ctggtgcatt cttctcagaa attgtcaaat caatatcaaa gtaacgttgt
     2821 aattccaaat gagaccattc aaaaatagga ttacctggcg ccttctccac tgtttttaca
     2881 aaagctaaaa atttttcata atcatcacct ggaccactga tcaactcttc ttctatacca
     2941 ttagctcgca tcaatcgcca tttataatga tctccagctc ctttatcatt taaccatacc
     3001 tgagtcaaat tcttgtattg cttattttct agcaaatatg ctgggtctaa atgacaatga
     3061 tagtcgataa ttggttggtg ttttgcaaat ttatgaaaaa gtcttttagc tatttcattc
     3121 tctaataaaa agttcttatc taaaaacata tttaaacact cctttccttc cgttagtttt
     3181 tagaagagaa aatttttcct ctaaataaac tcccttatat aattatttac tcgccccctt
     3241 ttcaagcgct tcccaaatac catttaaata aactgcacct agagctcggt catataatcc
     3301 ataaccaggt ctaccggttt ctccccaaat catccgacca tgatctggac gaatatagcc
     3361 attaaatcca ttatcgtaca ttgtttttaa tatcttatac atatctagag aaccatattc
     3421 tgatttatgc ggcgcttcgt aaaaatcgag tccattgtca cttaaatatt tgatattacg
     3481 aacatgtgca aaaggaatcc gatcccgttg acaaaattca gatagaattt catagacatc
     3541 attatctggt cgttctgcaa tacttccagt acataatgta ataccattag aaggagaatc
     3601 cactacatta cacaaccaat ctaaatcgtc acgattttta acaatacgag gtaatccgaa
     3661 aatggaatat ggtggatcat ctgggtgaac tgccatttta atcccaactt cttcacatgt
     3721 cgggataatt gctttgagaa aatatgcaaa gttctctcgt aatttctctt catcaacttc
     3781 tttataggct tctaataatt gcttaatttg tgataaccga tgaggttccc aacccggcaa
     3841 ttcaaaatta ttagtagaat tttctacttc tctaataatt tcctccggat cagtagggat
     3901 attttttgaa atataggcta acgtagaaga cccatcatct aaacgataat ccaaatccga
     3961 ttttacccaa tcaaatactg gcataaaatt ataacagata acctcaatcc caaattctgc
     4021 caaattgcga atcgtctgtt tataattctc aatgtattga tctcgtgttg gtaaaccaat
     4081 tttgatatca tcatgaacat taacactctc aataactttc aaggtaagtc cattggcttc
     4141 gacttcatct tttaaagatt taattcgttc tctcggccaa acctctccgg ctggtatatc
     4201 cattaatgtt ccaacaattc ctttcattgc tgggatctga cgaatttctt gtagtgagat
     4261 tttttcttca tttgccccat accatctaaa agtcatttcc atagttactt tcctccaact
     4321 tctgttatat atttattgat tgttttacga actgcttgag gacctactag catttcgcaa
     4381 aagatttttt cagttttctt tcctacccca acttgatata aatctaaacc aaagatttct
     4441 ttattttcta aaatttcctg taccgcacaa tgaatctttg aaggatcttg ttcacctaat
     4501 ttcaatactg ataatttatc ctgcaactct accaataacg gatcaggact tggcacaaaa
     4561 ttttcccctt gatcatcgac tgccagtaaa taacgtaacc aagcagcaat agcgatagga
     4621 ataaatgtta gattttctgc atttccgtct ttttcgacat actttttaat tgtttcacca
     4681 tagcgaatcg ctactttttg agaagtatct gttgcaattc gttgtggtgt atctggaata
     4741 tagggattag gcaaacgttt attaattact tcatcaataa attgttttgg atcaataatg
     4801 cctggatttt caactactgg taaaccttct aaataaccta ttcctttgat taaaccaacc
     4861 aaatcatcat cctgcatctc tgctgaaatt gattgatatc ctagcagaca acctgttact
     4921 gccaaagcag tatgaagtgg attcaagctt gtcgtaacct tcatgatgtc tgctttatca
     4981 accgtctctc tatctgtcat aatcacacca gccttttcca atttaggccg gccatttgga
     5041 aaactatctt caataactaa ataatgaact acctcagtat tagtgaaagc agcaatattg
     5101 gtatgttttt ttgtgtgaat gatatctaaa tcctcgagcc ccatcatcaa tagatcttcc
     5161 tttacattct ctgaaggatt tggcgtaatt cgatcaatca tactccaagg aaaagtcact
     5221 ttttctttat cctttaaata agcaataaat ttttcagaaa catatccttc tttttgccaa
     5281 gcttctgcaa tcgtaaaaat actatcttga aaacgttgac cattctgtga gaaattatca
     5341 gtagaaacca tcgcaatcgg caactctcca gcattaaatc tgctatataa caaggatgtt
     5401 acaatactca tcgtatgttg aggattgcta ggtccagatt tgatatcagc tttgactacc
     5461 gaaaagaatt gccctttaga atcttttaaa ctgtaacctt tttcagtaat agtaaacgtt
     5521 gtaaactgta atttaggatc ttcaaaatat ttaaccactc tttgatagtc cgctatattc
     5581 ttcggattgc aatataaact atcagccgtt actgccaaaa gtcttttttt gagttcacca
     5641 tctccactca taacaatttc caaaatgtca ttgttatagt catgataagc tttttccact
     5701 acttcttcat caaatgtttc acacacaact actcctgttt ctaattcatt ttgatctgct
     5761 aagcattgag caatttctgc atgaaatcct cgataaagat ttcctccacc aaaatgaagc
     5821 catactggat gttgctggcc ttcttgctta acttctttga catcaaaaca cggcagtaga
     5881 atctgcattt cattgaattc ttcgatagaa ttttgccaat ttttaatcga aaccataatt
     5941 aacctcctca aactgatatg ttagttttga tacattttat attttaaaac ccattgtaac
     6001 cgttgtcaat ataaaaaggg aataaacttg cttttttcta ccatatcaga atgattgtat
     6061 tttttattca gaaaaataaa atactccttc tttttggact gattttatcg aaacttattt
     6121 tttctttttt tcatgtattc ttttattttt ataactagtg tattacttca aaatttgata
     6181 ttatatggca tgaacagaat taaaatatca gttaaaataa aagttaagga gcattgtgta
     6241 tgacaattaa aactagtctc caaaatcaag cttaccaatc tattcgccga caaattatct
     6301 atgctgactt agaacctgga aaaaaagtat cagaaaaaaa attagaagaa acactttcta
     6361 ttggtcgtac accaatacgt gaagcactga ttcaactccg acaacaaggt cttgttgata
     6421 caattcctca aagcggaaat tatatctcat taattgattt agattcggcc aaaaatgctc
     6481 gatttgtcag agaacatttg gaacgacaaa ttatgttaga gtgttgtgcc aaaatgaata
     6541 accaaaaaaa acggatttta gaaactataa tttcagaaca agaaaaagct gttagtcaaa
     6601 aaaatgctcg tagttttttt cataatgata atttattcca taaagcttgt tttgaaattg
     6661 cagagcgaga agaagtatgg caatggttag aaaataacaa tacacactta gaacgatttc
     6721 gatggctacg agtaaaaacg gaaggattaa agtgggaaaa aattatggaa caacattatc
     6781 aactatttga ggcattaaca aaacaagatc cagaagaagc taactttcta acatcgttgc
     6841 atcttcattt aatgcttaat gagcaaaata ttgttttagc tactcatcca aaatatttca
     6901 aaaatatata acttttaact ctttttaaat taaatggttg ttctgatcag aaaa
//
LOCUS       DASJTC010000115         6780 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.118, whole genome shotgun sequence.
ACCESSION   DASJTC010000115 DASJTC010000000
VERSION     DASJTC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.118"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(106..564)
                     /locus_tag="U4B19_002478"
     CDS             complement(106..564)
                     /locus_tag="U4B19_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2078166.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(577..1908)
                     /locus_tag="U4B19_002479"
     CDS             complement(577..1908)
                     /locus_tag="U4B19_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEN2078167.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1919..3361)
                     /locus_tag="U4B19_002480"
     CDS             complement(1919..3361)
                     /locus_tag="U4B19_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2078168.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3348..4112)
                     /locus_tag="U4B19_002481"
     CDS             complement(3348..4112)
                     /locus_tag="U4B19_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEN2078169.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4216..5091)
                     /locus_tag="U4B19_002482"
     CDS             complement(4216..5091)
                     /locus_tag="U4B19_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2078170.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5346..5430)
                     /locus_tag="U4B19_002483"
     tRNA            complement(5346..5430)
                     /locus_tag="U4B19_002483"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5394..5396),aa:Leu,seq:caa)
     gene            complement(5448..5519)
                     /locus_tag="U4B19_002484"
     tRNA            complement(5448..5519)
                     /locus_tag="U4B19_002484"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5485..5487),aa:Gln,seq:ttg)
     gene            complement(5529..5601)
                     /locus_tag="U4B19_002485"
     tRNA            complement(5529..5601)
                     /locus_tag="U4B19_002485"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5566..5568),aa:His,seq:gtg)
     gene            complement(5663..5747)
                     /locus_tag="U4B19_002486"
     tRNA            complement(5663..5747)
                     /locus_tag="U4B19_002486"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5711..5713),aa:Leu,seq:caa)
     gene            complement(5768..5838)
                     /locus_tag="U4B19_002487"
     tRNA            complement(5768..5838)
                     /locus_tag="U4B19_002487"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5804..5806),aa:Cys,seq:gca)
     gene            complement(5884..5955)
                     /locus_tag="U4B19_002488"
     tRNA            complement(5884..5955)
                     /locus_tag="U4B19_002488"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5921..5923),aa:Gln,seq:ttg)
     gene            complement(5967..6039)
                     /locus_tag="U4B19_002489"
     tRNA            complement(5967..6039)
                     /locus_tag="U4B19_002489"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6004..6006),aa:His,seq:gtg)
     gene            complement(6042..6115)
                     /locus_tag="U4B19_002490"
     tRNA            complement(6042..6115)
                     /locus_tag="U4B19_002490"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6081..6083),aa:Trp,seq:cca)
     gene            complement(6122..6202)
                     /locus_tag="U4B19_002491"
     tRNA            complement(6122..6202)
                     /locus_tag="U4B19_002491"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6166..6168),aa:Tyr,seq:gta)
     gene            complement(6204..6279)
                     /locus_tag="U4B19_002492"
     tRNA            complement(6204..6279)
                     /locus_tag="U4B19_002492"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6244..6246),aa:Phe,seq:gaa)
     gene            complement(6315..6387)
                     /locus_tag="U4B19_002493"
     tRNA            complement(6315..6387)
                     /locus_tag="U4B19_002493"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6352..6354),aa:Asp,seq:gtc)
     gene            complement(6397..6469)
                     /locus_tag="U4B19_002494"
     tRNA            complement(6397..6469)
                     /locus_tag="U4B19_002494"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6434..6436),aa:Val,seq:tac)
     gene            complement(6485..6556)
                     /locus_tag="U4B19_002495"
     tRNA            complement(6485..6556)
                     /locus_tag="U4B19_002495"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6521..6523),aa:Glu,seq:ttc)
     gene            complement(6571..6660)
                     /locus_tag="U4B19_002496"
     tRNA            complement(6571..6660)
                     /locus_tag="U4B19_002496"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6624..6626),aa:Ser,seq:gga)
     gene            complement(6700..6773)
                     /locus_tag="U4B19_002497"
     tRNA            complement(6700..6773)
                     /locus_tag="U4B19_002497"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6737..6739),aa:Asn,seq:gtt)
BASE COUNT         2047 a         1498 c         1104 g         2131 t
ORIGIN      
        1 acaaaaatca aaatgattaa atctagtgat tgaaacatat catttcaaaa aaattttgta
       61 aattcaccca taaatatcac cctatttaac ttttttcatc gtacactaaa attcaaaaca
      121 actatcttta atctttactt cttggttttg ttttaataaa tttccttcaa aaatgcctgg
      181 agaaactaca atgatcaccg tatctttttg tgcttgctcc aacttcttca aatctataat
      241 agctaacagt gtcccttctt ttacctcctg gttactttca acccgtatct gaaacggact
      301 tccttcgagt tcaactgtat ctaaacccat atggatcagt atttttctgc cagattcact
      361 ttcgatcgtt atggcatgtc gggtaggaaa tatattatcg attattcctt taactggcgc
      421 atggatatat ccatcatgat tcgttatagc gtatccaact cctatcattt ttgaagaaaa
      481 gacctcgtct tttactgctg ttaatggaat aatttgacca ctagctacag cttttagaat
      541 gtttcttgtt ttaaagaaat gcacttttta tcacctctac tttaatgctg gccaatattt
      601 tttgtttact ttgatcattt catctagtat tttttttgcg actctggcat ttggtacggt
      661 ttgattcaat gtaaatgctt gtaaagcttt ttgataagaa ccttcaaagt aagcatctac
      721 tagtaacttc tctactgcaa cttgtgcctc catcaatcct ttatgaaaat cactgattgg
      781 ttttcttagt gtaattggct ccacaccagt tgcaccaaca tatgccggta tttcaacaac
      841 tgcatctgac cgtagattcg gaatcgcccc ttgattagga acgattagca taaagcgacg
      901 acgatcatca ttcaacaagg aaatagctat atcgacgata tattgtccat gctccccaaa
      961 attaaagttc aatacttctt ccacaatacc gcggcgtatt ttatcagcca tttctttggt
     1021 atttttctct cgtccatcca tgaccatatt agcacgtgta tactctttgt cagtatgctc
     1081 aacgaccata tcaggatata agtaatactc taagtaatta ttcgggatat tacttggaaa
     1141 atttttcgtg atgactgaca tcatttgaaa ggttttttgc caagttttat ctcctatatt
     1201 aaaatcggcg acttgcattt cttttgttat tagtttttca ataatctctg gcataatttc
     1261 tcgttgcaat tctttatcat aaatcgatgt ataccagcca aagtggttta aaccataata
     1321 cgtaacgatc caattcttac gatcataacc gtaattcact gcaatcgttt cttcaattga
     1381 aatagtcata tcacaggcat taatcatttt tacattcgga tatttacgac gaacagcttc
     1441 agaaacgatc gactctggat ttgtataatt caaaatccat gctttggggg catagtcttg
     1501 tatatgatcg acaatttcca ataaaccttt gattgagcgc atcccataag caaatcctcc
     1561 gagtccacac gtttcttgtc ctactaatcc atgtttaagt ggaatctttt cgtccttttc
     1621 tcgcatctcc aatcctccaa cacgaatttg tgagaaaaca aaatcgcatc ctgtaaaagc
     1681 aagctttgga tcatccgttg cacgaatgcg aatatttgaa aaaccttttc ttttcagcat
     1741 aaatttaaca atcaagtaca tatccatatt tctttcttca tcaatatcat ataaacgtat
     1801 ttcagataat ggcaaccgtt tttcattgtt caaaatggct tgaacgattc ctggtgtata
     1861 cgtactcccg cctccagcta tagtgataat ctgttttttc atcatcatct tttcctttct
     1921 attcttctaa taatttattg aattctgcca taacataagt cacttctaag ccgatgataa
     1981 tttgataatt tttttcagtt ggtttaatga tcccgcttat ccctaattgc ttgattcgct
     2041 gttcctcgac catggtggga tccactacct ctacccgtag gcgtgtatta caactataga
     2101 catccaggat attggctttt cctccaaacg cttgtagcat ttttgttgcc atatattcat
     2161 aatttttact tgctaaccct aactctgttt cttctaaggt tacttcattg ccaaactctg
     2221 ccttagcttc acgtcctaat gtctgaatgt ttctcttatt gatcaaataa taaaaagtaa
     2281 cgtaatacaa ggcaaaaaag aaaattccga ttggaagaat caaaatagca ttggtagcga
     2341 ttcgaaaatt gatgagataa tcaacaatac atgctccgaa tgtaaaacca attcgtatat
     2401 tgaataagta acagacaatc cctgctaaac cggcaaaaag tgcatggatc acgtacaaca
     2461 taggagaaag aaacatgaaa ctaaattcaa caggctcagt gatatttgca ataaatgaag
     2521 ttcctgcacc gcttcccatg attcctttga ccatctcttt attttcttta taagctgctt
     2581 tatagatagc tagacatata gctggtatac caaacatcat tactacaaaa aaaactgata
     2641 aaaacagtcc agcctgagga tctccgttga taaagcgtgt catctctcct ctgaaaactt
     2701 ccttgctagg agatgtatag cttcctaaat cgtaataaat atacgtattc aacacatggt
     2761 gcagtccaaa ggggatcagc aaacgattca aaaacataaa tatccccaca cctaatgctc
     2821 ccatatcaac gagcagtttt gcaaaattat caatcccaat ttggatgagc ggccagataa
     2881 agcccaaaaa aacagctgcg attgtttgta gaatcatcac catagtcaat gggaattttt
     2941 cgcccgcaaa ataagaaaaa actactggta atttttgttc tttaaagcga ttatacgtcc
     3001 atgccgctac taaaccggaa ataattccac caaaaatccc catattcact tctgggtcca
     3061 taatttctaa gccttttttc aaggtagcaa tcgctaaaaa accagtaagt gcaggtattc
     3121 ctttgtcctt atttttgaca aaaccgattg caactcctat agcaaataag atatccaagt
     3181 ttccaaaaac ggcatttcca gctgcaccaa ttactgggat gttcagcata tcgtcagctg
     3241 ataaacgcat gatcaatccc gctaatggca tcgctgcaac tggaagtagc atcgctcttc
     3301 caagcttctg cacatttgat ttaacattca tttttattcc tcctctatta aatatcctca
     3361 tatgttacta actcaatcaa aggatcattt ttcacttttt gagcaaatgc tgaatgaatt
     3421 aaaaattcga cttggtgggt acgaggatac acaaaacttg aaccatttaa caattcctga
     3481 tccaaatacg ctgcgtgaca catgacttcg atcgttttat cttctctcaa tatcatgagt
     3541 aaacttgcca aatattcttc cacactcgta ttgatttgtc gttttttttc atcagtttct
     3601 cctacgacat cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc ttttcgttca
     3661 aagttgctcc gaaccggcaa atcatatttt tttgctaatg taacaactac ctcctgattt
     3721 tcatccaaag tatgagtatg atgatgactg tctaaatgag ttggtatgat ccccgcatga
     3781 taaatttttt gaatctgagc attccattcc tgatacagtt gatctttgtc aatgttaaat
     3841 ggatgctgat aataagataa ggatctgaac tctccgcttt cgataagagt atcgacagtt
     3901 ttgaggagtg gtttgccaca cgtcaatgtt aaatggactc ctacacctag cctaggcatt
     3961 tcctttctta gtttcacagc atgctcgaaa cccggcatat ttgccatcaa tgtagttgat
     4021 gtcaaaattc ctcgctgata agcatccata atcccataat tcacaccatg gctatagcca
     4081 aaatcatccg aattaaaaat cacttttccc atttcattct ccttcttttt tctactaaaa
     4141 taggcgcaac caaataatca tagtccattt actatgtgtc atttggttgc gcctatagat
     4201 agttacgccc ttattttaat agattcttgc tagatgaatt gccaaataga tgatttcatt
     4261 ttcttgaatg gcaagttgaa attgctgttc gatgatatct ttaatcttta gtgctgtaca
     4321 atacgctttg ctatgtttaa atgtttcagt aaaaaaacca atattttcta ctggcgtttc
     4381 ttcctgataa atcaagcgtt taaccaggaa tttgatatga accattaagc gatgataagt
     4441 cagtgattct ttatcataaa cgtttgaata ggatttttct agatgggaca aaatcgtggt
     4501 cgtcatttct actattttta agctctttaa actattaggc tctcctagct cagcattaat
     4561 gatatgcata gtgatgaatc ctacttcatt tgaccctaac tcggtttgat atcgatgatt
     4621 gattcgctcg accacttcta ctgctatatc atattcattt ggataaagga atctcatttc
     4681 tggcaacata attgctgtga catctctatc ttctcttttt cgttgaatag caaaataaat
     4741 atgatctgtc aatgtaatat agatatttga atctaatgga gaggagatcc tagctgttat
     4801 cagttcaatc acttcttccg caaaatccat aaattcgatt ggaatctgcg agactatctc
     4861 actgaatcgt ttgatctttt ctttagaatc taatacaaat aatttgtcag catcctcaac
     4921 atcaaatggc ttactcactt tagcagaaaa gccaatccct ttccccatcg caattttttc
     4981 tattccatcg tcatcgatca atacagcatt atgattcagc tttttcatca catacaccgt
     5041 taattcacct cctctatatg ataaaacaaa acaacttgca gactccttca tacatatttt
     5101 tgtctccaaa caatttttaa agacaaaaaa cgacctaaga aagaaccctt tcttaggtcg
     5161 tgcctaattg aatagtagca ccctacataa tttacaaaaa tagtttaaca cataataaaa
     5221 gcgttatcaa tatctttttt actcgtttta tgataatact tgtatcgaat tgatttggag
     5281 gtaattacat aaaaacaaaa aaaatatagc aactctctat aagaaagttg ctatattgat
     5341 aattataccg gcggccgggg tcgaaccggc acgtcctcaa cggacactgg attttgagtc
     5401 cagcgcgtct gccaattccg ccacgccggc aaatttaaaa ataataactg gggtagctgg
     5461 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
     5521 gaataaaggg cgaatgatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt
     5581 taaccacttc gccacattcg ccatcataaa agatgttatt taatttctca ataaaaaact
     5641 aagatgaaga catcttagtc aataccggcg gccggggtcg aaccggcacg ccctcaacgg
     5701 gcactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaag ttgatttatt
     5761 agtactaagg cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac ctctgcctta
     5821 ccacttggct ataccgccat tatactatta tcaatctata aaataaagaa taaattatag
     5881 aaactggggt agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg
     5941 cttggctata ccccaagaat aaaaagggcg aatgatggga atcgaaccca cgaatgccgg
     6001 agccacaatc cggtgcgtta accacttcgc cacattcgcc atggcagggg cagcaggaat
     6061 tgaacccaca ctaacggttt tggagaccgc tgttctacct ttaaactatg cccctataaa
     6121 atggaggaga gtggattcga accactgaac cctaaggaac ggatttacag tccgtcgcgt
     6181 ttagccactt cgctactcct ccatggtggc gcgggacaga atcgaactgc cgacacacgg
     6241 agcttcaatc cgttgctcta ccaactgagc taccgcgcca tattttaaaa ttattaattg
     6301 aataacaaga acgacggttc cgacgggaat cgaacccgcg atctcctgcg tgacaggcag
     6361 gcatgttaac ccctacacca cggaaccagt tcattatgga ggttaacggg atcgaaccgc
     6421 tgaccctctg cttgtaaggc agatgctctc ccagctgagc taaacctcca acattcaatt
     6481 aaaatgaccc gtacgggatt cgaacccgtg ttaccgccgt gaaagggcgg tgtcttaacc
     6541 gcttgaccaa cgggccaggt aaatttgcta cggagagtaa gggattcgaa cccttgagac
     6601 agtttggcac catctacatg atttccaatc atgctccttc ggcctctcgg acaactctcc
     6661 agagtgaaag aagctcaata ctctttctca aaatgagaac tccggcagta ggactcgaac
     6721 ctacgacatc atgattaaca gtcatgcgct actaccaact gagctatgcc ggaataacat
//
LOCUS       DASJTC010000116         6677 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.119, whole genome shotgun sequence.
ACCESSION   DASJTC010000116 DASJTC010000000
VERSION     DASJTC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.119"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            193..2868
                     /locus_tag="U4B19_002498"
     CDS             193..2868
                     /locus_tag="U4B19_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEN2078171.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3050..3289
                     /locus_tag="U4B19_002499"
     CDS             3050..3289
                     /locus_tag="U4B19_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078172.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3305..4678
                     /locus_tag="U4B19_002500"
     CDS             3305..4678
                     /locus_tag="U4B19_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2078173.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4682..6316
                     /locus_tag="U4B19_002501"
     CDS             4682..6316
                     /locus_tag="U4B19_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2078174.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6479..>6677
                     /locus_tag="U4B19_002502"
     CDS             6479..>6677
                     /locus_tag="U4B19_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078175.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2344 a         1074 c         1338 g         1921 t
ORIGIN      
        1 tcctaaataa tttaataagg attgatttat cgtaaaagaa aaaagttgtg atttccaaaa
       61 tcacaacttt tttcttttgt caagattttg agtgggatat atttttgtag aactgaagat
      121 catgaaatta tgagaggttg gaggtaatgt agtcatacaa ataaaaattt tttagaaagt
      181 gagtggtaat taatgaaaat gaatcacact tcaattcaga agcatttaca actgttaaat
      241 acactgggga tttcttatgt taatttgaga actttttcta cattaaacaa gaaaaccgtt
      301 tctcatattt tttctactgc agattctcaa gcaattatta actatttaga aaaacagaaa
      361 ttgtctgaag gtacagtaaa cataacttat aatcctatta aacaagaggt gattacacaa
      421 aaaaagcatt ctgtgagaga caatgatatt gaaaaaattc ggtttgtatt tattgatatt
      481 gaccctaaaa ggaaagaggg aagtgcaaca gatagcgaaa agaaaaaggc agaaaatgtt
      541 atggaacaag tagaacgtta tttgaaagaa aagagaatag aatcatttgt aaaagttgat
      601 agtgggaatg gctaccatat ttttcttccc ataaatgaac aaccaaataa tcacgagacg
      661 attcttacta ttcagaattt tcttcaatta cttcaccgaa gatttggagt agaagatgaa
      721 gttgatattg atacttctgt ttataatcct tcaagattat gcaaattagt tggtacacca
      781 gcggtcaaag gaaagtccac aaaagagcgt ccgcatcgtt tatctagttt cctttctatt
      841 cccgataaca tagatagaag taattgtttt aaaattcttg aacagatagt tgaagaaaat
      901 tccatgatgt caacctctag agtaaaaagc catccatctt ttagagaagt gttacctttt
      961 attcaagcag actataaaca atggttagct cactatcctg aattaactta taccataaaa
     1021 gagggcgatc ataaaggagt taccttactt atatttgacg aatgtccatt aagaaaacac
     1081 acgaataatt ctaatggttc ttgtttgtct gttacggctg ataataagat tcgatttcat
     1141 tgtctgcatg cttcggataa aaataaagat attcaagact ttgtaaaaaa atatccgttg
     1201 ccaaaacctg taaatccttc tgtagaatca ttagaatcca atcaagaata ttattttgac
     1261 cagtttaaac tgaatcaaga aggagtattt ttggtgaaag aaggtcaaga agaaaaagtt
     1321 gcttgtccga tgtttattaa gcaaatacgc aaaaatatcg aacaaaaagt cattacactt
     1381 gttcttgcct attatgtcga tcattgttgg gatgaaatag aaatagaagg tggatgttta
     1441 caaactaata attttaaaca attagcaaaa catggcttaa tcattaaagc tagaagagaa
     1501 gctgaagtcg ttgacttttt acagattcaa cgggagaatg ctccaatctt ttttgaacat
     1561 gaatctcttg gctggaaaaa agaaaatgaa gcaatgattt ttcagttaca agattctttt
     1621 ggacgtgaaa ttccttctgt attaactaaa aactcatgct accaattaac aactaatggt
     1681 acagaagcgg aatttgacgc attggttcat aagtatgttt taggaacgaa cttagaactt
     1741 gcttggtcga ttggtattgc ttctattgtg ttaggctatt taaacgttac gaaggctaat
     1801 acttttttga gtttactaat taacctacag ggcaagagca caacgggtaa aaccactacg
     1861 ttacacttta ttgcctcttt atttggaaat ccaactacgg ttcttagaaa tatgaatgct
     1921 accaataaag ccattataaa gctagcatca aacaatcagg gaggtgttcc actaatacta
     1981 gatgagttag gagcagcaac tcaaaataac tttagctcgt tgatctatca attagcttca
     2041 ggagaagaaa ggctaagatt agattctaat tgtcagctta tgccacaaga gactttttct
     2101 gttttgaccc ttacctcttc tgaagagcgt ttggaaaatt atttcaatga gaaattaact
     2161 ggtttgaaag ttcgatatat cgaattttct gatatcaagt ttacaaagga tgcgaatcac
     2221 gcagagaaag ttaaaagtct ttcttctaat acttttggtt ggggtataaa gttatttctt
     2281 ttgaaactct ttgaacaagg gattacaaaa attgaagcaa cttttgtcga agctcaaaaa
     2341 aaactatggg ctgttttacc tgaacattct ttgaagcaac gcattggtaa taatctagct
     2401 ttgatttata gtggagccct cctaattcaa gatatactgg gctatccttt acgattagag
     2461 catatacaga cggtgcttat taattcgtac gaacagttaa ttgtagatac tagtttagga
     2521 agtgagcaaa cggtatttga ccgagtgaaa acattgttgg tgatgaatag ccataaattt
     2581 ttaacaaaca gtgacaacaa acatacgccc agtacgattt ggggaaaagt atcaattcaa
     2641 aaaaatgggg atattaaggt aaatattttt cctatcccgt ttgaggactt gttaaggcga
     2701 gaatttcaag taaaggatct aacctatctt tttaggcaat tgcttgattc gggtaatatg
     2761 tgtgcagaaa aagggagaag aacgaaacgt attcacatga atcgtcaagt tcttccaaca
     2821 tacgagattc tattaccttc ttcactcaaa tcgtattttc gattgtaatt tagaataaac
     2881 caaagtatga aagtgaggca ccatgttatg gaaatatttc ttattgattt agcaaaacaa
     2941 ctgattactt atctgtatga tagcgagcaa acccaaacgg aagtaaatga agcttgatct
     3001 atgcttcatt tacttcattc aatcaaacat gaaagaaagg aaaattctaa tgactaaaaa
     3061 taaaatgaca ctaaaagcag aggttcttct gtatattcaa gaacattttt caaatcaagc
     3121 cttttttaca aaaccaattt acttggattt cgaaataaga ggggtatctg ctggatctat
     3181 cgggggtact cttcaagcgt tgaagaacga aggatattta gaaaatcact ttgtgcaacg
     3241 ttcgttcaat agaagagtcg tgaaaaaatg gtatttagtt cattcataga gaggggaatc
     3301 aagcatgcgt gtggcttgtt atgatcgaac gtctgtttat gaagaagcga aaattagtaa
     3361 tgacgctcaa tatgatgcag tgaaagagta tgttttagag aaatatgaac aaattcccca
     3421 acgattttca gacaaaggag aatcagggga aaaagcggag cgtccagctt ttcgacagct
     3481 tagagcttgg gtttgtgcgt cagaagaaga gggggtgatc tatgtttggc gttatgatcg
     3541 attggcacgt caagccaaaa tagccttaga atttattgag ttgtgccaac aacatcaggt
     3601 tcaagtgtat tcgatttctg aacctcttcc tgcttctttc tcacacgcgt caacgaataa
     3661 actatttgtg caattattgt tcacttttgc agaacaacaa cgaaacgtca ttattgagaa
     3721 tgttcgtgcc ggactttcct ataaaaagca acaacggcaa tatttgtctt ctgcggtacc
     3781 atatgggtat cgtttagtga atgggaaaat ccaacaagaa gtacacgaag caaggattgt
     3841 tcgctatatt tttcaacttt atttgaccaa aaaatatggc tacaaaaagc tttgtcaacg
     3901 attgacgcaa caaaagcttt tcttccgcga acgtcctttt cagccctatc atatttatag
     3961 tatcctaaaa aatccattgt actatggcga aatcaaagga ggatctttgg ggaagtatct
     4021 aggaaccttt gaaccgattc tctcgaaagc aaccttttta caagcccaag agattcgcca
     4081 atcaaggtgt acagctaaaa aagatacgta tctgtactta ttacgtcaaa aaattcggtg
     4141 tcccttctgt ggtcgtcatc tgagttcgaa gtatcaatgg aatacaaaga agacgaagac
     4201 gcttcattat tatcattgta ccgaccgaag ctgccaagga atttttcttc gggcagaaag
     4261 ggttgagtca gaagtattgg aaaagataca ggcttttttt caatacgaag gcgtctatca
     4321 agccttgatt gcccaattaa cgcaacaatt agccgaaaag cgaaaagatg aaaaacgcaa
     4381 agtaatccaa caaaagaaac aagaagcaca atggattaag gcatttgaag agggcaattg
     4441 ttcactggaa gaactcaagc aacaactgca agctcttcaa aagccgatgt ctacttcttc
     4501 gtttctttca ctccaacagt gtgaagcaaa agtgaaagaa tggcttcaac taaaagacct
     4561 ctctagtcga caattattat gtgactatgt ttgtcggatt gaggtaacaa aggataaaac
     4621 agtttgtggg atttacttcc gacaactgac acaaaatatt ttagaaagga acgaatgatc
     4681 tatggaaatg atacaaacca aacgagtcgc attgtatatt cgcgtgagta cacaagaaca
     4741 agctttagaa ggacatagta ttcaagcaca agaacaaaat ggacgacagt ttgccgaacg
     4801 gatgggctat gaagtggtag aagtttatgc cgatgaagga atcagtggga aatccacaaa
     4861 aaatcggttc gcctatcaac gaatgatgaa cgatgctcgg aaccatcgtt ttgatctact
     4921 agtgatttgg aaattgaccc gtctcggacg caatatgtta gatgtcttac aaacagtcga
     4981 ggaattattg cgatatgacg taggattaca ttcgatctcg gaacaatttg acgtgaccac
     5041 ttcgacaggt aaattgatgt tacaactcct tgggagtttc ggtgaatttg aacgcaacca
     5101 aattgcggag aatgttcaaa tgtcaatgca aagcttagtg aaagataaaa agcgttatgc
     5161 aggaggccga cgtttaggct atgtcagtgg tattgacacg aatggtcaaa aacaattggt
     5221 cattgaacca gaagaggcga aaattgttcg tctgatctat gcaaaatatt taggcggagc
     5281 agggtatcga gcgattgcca attttttgaa tcgtcaagga tatcaaacag tcaaaaagaa
     5341 tgctttttct accacggcag tcaaagatat cttacgcaat aaaatttatg cagggtattt
     5401 agaatatgcg cgctacatca attgggaaac caaacgacgg aaggggaaga atccgagacc
     5461 gattttagtg aaaggaaccc atgaaccgat tatttcggaa gaagattatc aaaaagtaca
     5521 agaacgcctt gctttagaag gaaaacaacc caaatggaac catacaggtg aaaatgtatt
     5581 gacaggcttg ttacgttgtc cagaatgtgg cgcaccgatg gcagcgagta atgtcacaaa
     5641 taccctaaag gatggcacaa agaaacggat tcgatattat tcttgttcag tctttcggaa
     5701 taaaggagca agtgtgtgtc atgccaattc gattcgtgcc gaacaagcgg aaacatttgt
     5761 cgccgaccga ttaaaggaga tcgttcagct accagaagtt cttccccgat tggtggcagc
     5821 gttaaatgaa gaaatagtgc gacaaagcca accattggaa caagaactcg tggtgttact
     5881 ggaacgaaag gaagaattga agactaaaat tgaaaaatgg gaagctgctt tggaagatag
     5941 tcctgaattg tttccgatgt taaaagaccg cttagacgaa ttgactgaaa aacgacgaca
     6001 gttgcacatt cgagaaaatg aaatcttagg gatctttcaa caaggagagc cgattcaagt
     6061 caaagatgtc caacggatat tgactagttt ggatcgtttt cttgcccaga gtgagaagaa
     6121 gcaaatcaaa gccttgtatc gtacctttat agagaaaata acctttgatc ctaaccataa
     6181 ggagatgttg gaaattacga tgaaattcac gcatgccgtg gtacaacaat tgaatgagca
     6241 atatcaaata gccgtatcca aaacaaagga tacggctatt tttgtgttaa aaacaccctt
     6301 tacgttaacg atctagtcta ggttatatca aacaacgtta gaggggcgct aacgcttctc
     6361 tagcgatttg aaatcgttag agaagtagtt agggaggtcc ttatcataaa tagggatatt
     6421 tattaatgaa tatttgaaat ataattgtat ataagaatta aaaagagtgg ggatagatat
     6481 gaaaaagatg ggtaagtgta ttttatgtga aaaatacact gaacttacta aagaacatgt
     6541 accacctaaa aaatcaggga atactggagg taaaaagacc agaactggta atttagatga
     6601 tttccttaag agcgatttta caaaaggtga ttttccgaag gggattaaaa ggaaacctca
     6661 aggaaatgtt tattata
//
LOCUS       DASJTC010000117         6616 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.120, whole genome shotgun sequence.
ACCESSION   DASJTC010000117 DASJTC010000000
VERSION     DASJTC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.120"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1273..1938)
                     /locus_tag="U4B19_002503"
     CDS             complement(1273..1938)
                     /locus_tag="U4B19_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2078176.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2118..4100
                     /locus_tag="U4B19_002504"
     CDS             2118..4100
                     /locus_tag="U4B19_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEN2078177.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="U4B19_002505"
     CDS             4104..4742
                     /locus_tag="U4B19_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2078178.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="U4B19_002506"
     CDS             4770..5198
                     /locus_tag="U4B19_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078179.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="U4B19_002507"
     CDS             5655..6566
                     /locus_tag="U4B19_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN2078180.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2276 a         1003 c         1220 g         2117 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatc
//
LOCUS       DASJTC010000118         6449 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.121, whole genome shotgun sequence.
ACCESSION   DASJTC010000118 DASJTC010000000
VERSION     DASJTC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.121"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            142..558
                     /locus_tag="U4B19_002508"
     CDS             142..558
                     /locus_tag="U4B19_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078181.1"
                     /translation="MQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPE
                     IHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSKYNLLEYD
                     LIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(631..1278)
                     /locus_tag="U4B19_002509"
     CDS             complement(631..1278)
                     /locus_tag="U4B19_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEN2078182.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(1286..1972)
                     /locus_tag="U4B19_002510"
     CDS             complement(1286..1972)
                     /locus_tag="U4B19_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2078183.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(1978..3051)
                     /locus_tag="U4B19_002511"
     CDS             complement(1978..3051)
                     /locus_tag="U4B19_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2078184.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            3273..3947
                     /locus_tag="U4B19_002512"
     CDS             3273..3947
                     /locus_tag="U4B19_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2078185.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3952..5292
                     /locus_tag="U4B19_002513"
     CDS             3952..5292
                     /locus_tag="U4B19_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2078186.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(5441..6427)
                     /locus_tag="U4B19_002514"
     CDS             complement(5441..6427)
                     /locus_tag="U4B19_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEN2078187.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         1955 a         1241 c         1124 g         2129 t
ORIGIN      
        1 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
       61 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
      121 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
      181 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
      241 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
      301 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
      361 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
      421 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
      481 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
      541 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
      601 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
      661 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
      721 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
      781 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
      841 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
      901 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
      961 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
     1021 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
     1081 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
     1141 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
     1201 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
     1261 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
     1321 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
     1381 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
     1441 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
     1501 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
     1561 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
     1621 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
     1681 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
     1741 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
     1801 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
     1861 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
     1921 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
     1981 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
     2041 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
     2101 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
     2161 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
     2221 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
     2281 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
     2341 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
     2401 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
     2461 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
     2521 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
     2581 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
     2641 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
     2701 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
     2761 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
     2821 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
     2881 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
     2941 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
     3001 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
     3061 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
     3121 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
     3181 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
     3241 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
     3301 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
     3361 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
     3421 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
     3481 aaatacagac tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
     3541 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
     3601 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
     3661 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
     3721 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
     3781 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
     3841 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
     3901 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
     3961 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
     4021 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
     4081 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
     4141 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
     4201 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
     4261 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
     4321 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
     4381 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
     4441 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
     4501 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
     4561 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
     4621 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
     4681 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
     4741 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
     4801 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
     4861 caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat
     4921 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
     4981 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
     5041 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
     5101 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
     5161 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
     5221 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
     5281 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt
     5341 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
     5401 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
     5461 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
     5521 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
     5581 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
     5641 atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
     5701 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
     5761 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
     5821 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
     5881 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
     5941 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
     6001 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
     6061 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
     6121 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
     6181 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
     6241 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
     6301 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
     6361 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
     6421 cttccatgta aattcctcct agcaaatag
//
LOCUS       DASJTC010000119         6411 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.122, whole genome shotgun sequence.
ACCESSION   DASJTC010000119 DASJTC010000000
VERSION     DASJTC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.122"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            20..799
                     /locus_tag="U4B19_002515"
     CDS             20..799
                     /locus_tag="U4B19_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078188.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKNFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISAAYATKAQTVAQQMGYSNISY"
     gene            845..1789
                     /locus_tag="U4B19_002516"
     CDS             845..1789
                     /locus_tag="U4B19_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEN2078189.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTLQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1805..2584
                     /locus_tag="U4B19_002517"
     CDS             1805..2584
                     /locus_tag="U4B19_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEN2078190.1"
                     /translation="MEETISLKEIFETLRKHLVTIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            2596..3294
                     /locus_tag="U4B19_002518"
     CDS             2596..3294
                     /locus_tag="U4B19_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEN2078191.1"
                     /translation="MARTQKQKATQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSRDAGYYYGN"
     gene            3322..4086
                     /locus_tag="U4B19_002519"
     CDS             3322..4086
                     /locus_tag="U4B19_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEN2078192.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLQLGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVAQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            4099..5916
                     /locus_tag="U4B19_002520"
     CDS             4099..5916
                     /locus_tag="U4B19_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2078193.1"
                     /translation="MFVLTRRRKIATLVFVDSLLLIAANIAAAKFMKPFVAIPMDLIL
                     ISIGLSIGFYLFYGSLFKVFTRINRYTNLREIIAIFGSLSASAASSIIILLFINKQYS
                     LRLVIFAYLLSLLLIIGSRLIWRIYIETKNMRYLSTDSAKNTLIVGAGEGGRILYNSF
                     LGSKTAQDIHVIGFVDDDPNKRNTYLSGKKVLGSLKDIPELIEKYDIQMVTIAIPSLS
                     RKKLRRIFELVESAHVKVNTMPSMEELASGKISVSKLKTIDVVELLGRDEVELDIESI
                     KDQITNKVILVTGAGGSIGSEICRQIIQFDPAKLLLLGHGENSIYLIDRELRTHHQKC
                     PTEIVPIIADIQDRKKINEIMEQYHPDIVYHAAAHKHVPLMEYNPKEAVKNNIFGTKN
                     VAEAAKAAKVKNFVMVSTDKANNPPNVMGSTKRIAEMIVTGLNEEGCTKFSAVRFGNV
                     LGSRGSVIPVFREQIAQGGPVTVTDFRMTRYFMTIPEASRLVIQSGALAKGGEIFILD
                     MSEPVKIVDLAKNMIRLSGYSEDEIEIIETGIRPGEKLYEELLLDKERNDEAVYEKIF
                     VGNIKGYSMQTVMEFVKSLPQDDEQLAKDIVTFANASNK"
     gene            5900..>6411
                     /locus_tag="U4B19_002521"
     CDS             5900..>6411
                     /locus_tag="U4B19_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN2078194.1"
                     /translation="MLRISRGSKVKAIIVGAGGFGKEIAFLLESFSRYELIGFVDDSL
                     KMQNQELVGKTVLGTIDSLLELEEETAIFLGIASPDIKEKIYQKINKNNKLIFPNLVA
                     SSALVGINVQLGIGNILMPYTTYTADVVLGNFNMINIGSTIGHDTQIGNYNSIFPSVN
                     ISGHVLLGDK"
BASE COUNT         2235 a         1181 c         1347 g         1648 t
ORIGIN      
        1 aatacttagg agtgaatcta tggaaaagcc attcgtcaag tcattgactg ctgtggcggt
       61 aggtgcaggt gctattgcga tctgtttgtt tggttaccat atcaataatc agcggcaaca
      121 tcagcaacgg atcaattatg cagaatctgc catcaccaat caaaaagaca ccttgacttc
      181 tttatcaaaa gaagtagaca agctgtatag tacaaaagaa aaaaattttc taaaccctga
      241 aataacagaa gaaacaatca caagtttgtc aaataagcta tcctctgtca aactttcagc
      301 agatgattac gacatcaaag aatcagaatt gcctaaagaa gctgttgcaa tccaagatga
      361 gaagaaagca atcgtgacac agttagatag tgcagaatca aaactgaaga tccagacagc
      421 tgtcaacgat ttatttacta aaaatgtatc aaactggcag caagctgtag acgatgtcgt
      481 catcaaagaa aaggtgacaa gtgcagatgt cgctcgtgta cgtgaaaata tgagcttttt
      541 taaagatagt ccttggaaaa cggttgttat gcaatatcta ggattcgctg atactcagat
      601 cgcgcaagtc acgcaactag atcagttatt cgatacgatg ttgaaggatg gtcaagtgac
      661 tgccaccgct acttacgatc aatatttgac ggcgctcagt caaatagagc agatccggaa
      721 tgaaaaaata agcgcagcat acgcaacaaa agcacaaact gttgctcagc aaatgggcta
      781 tagcaatata agttattgac agttaaaacg ttgattcaag caaggttagc aaacggagga
      841 aactatggca agacgaagaa gaaaatcaag agggaaaaaa atatttctga ttattttagc
      901 agttcttatc gtattagtat tagcaattgt aggagtagga gcaaaattat atctggatct
      961 ctcacattct attgagaaaa cttacgaatc agtggaacga acccaaggag aacgaacgaa
     1021 accagttgat ctggacaaac aagaatcttt ctctgttcta ttgatgggca ttgatactgg
     1081 agatttaggt cgggtcgatc aaggaagatc agatacgatg atggttgcca cagtcagtcc
     1141 tgaagacaaa cagacaacga tcgtcagcat tcctcgagat acgtatgtcg acatcgtagg
     1201 tcgtgacaca aaagacaaga tcaaccacgc ttacgcattc ggcggagctg ccatggcgat
     1261 ggatacccta caaaaatatg tagacatccc aatcgatcac tatgtctcta tcaatatgaa
     1321 agggctaaag gaactcgttg atgcagttgg tggtatcgaa gtcaataatg acctcacttt
     1381 ctcacaagat ggctatgact ttccaatcgg taaaattaca ttgaacgggg atcaggcatt
     1441 atcttattct cgtatgcgtt atgaagatcc aaatggtgac tatggtagac aagagcgtca
     1501 aagaaagatc gtagaaggtg tagcaaaaaa agtattgagt ttaaacggtg tcagcaacta
     1561 tcaatccgtt ttgaatgctc tagagtcgaa tatgaaaaca gacatgagct ttgatgaaat
     1621 gaaaaaaatc gctttagatt atcgggaggc atttggtacg atcaagcaag atcagctgca
     1681 aggagaaggc ttcatgcaag ataatatctc ctatcaaaaa gtcagcgatc aagaacttga
     1741 tcgtgtgaaa aaagaattaa aagatcaaat gaatctagag aataaatagg aaagtggaga
     1801 aaaaatggaa gagacaatca gcttgaaaga aatttttgaa acattacgca aacacttggt
     1861 aacaatcatc atctcaatgt ttgctggctt ggccatctca ggaatcgtaa catttttcat
     1921 cttgacacca aaatacagtt cacaagcaca attgatcgtg acactgccac aatcagacac
     1981 agcaaatgtc aatgatgtca atacgaacct gcaaatgatc aatacgtata aagatatgat
     2041 cgtcagtgat ttagtactaa atgaagtcaa agatcgttta gaaacagaag acaatgtgaa
     2101 aatgactgct ggacaaatca aagatgcgat ctccgtcaat cagtcacaaa attcacaaat
     2161 gttctcgatc caggcaataa gcacgaatgc agtgactgct caacagatcg ctaatacaac
     2221 ttcacaagtt ttccaagaaa acgccaaaga cgtgatgaat gtcgataaaa tctcgattat
     2281 ttcaggtgca gttgccaata cgacacctgt ttcgccaaat aataaattga atctagtgat
     2341 tggcttagtg ttaggaatga tggtaggcgt aggtctggca ttcttattcg aattacttga
     2401 tcggacagtg aaagatgaaa aatatgtgac ggatacatta ggattcccaa tcttaggaac
     2461 agttccagaa atgtcagcaa aagaattgaa cgctggtatc cgtaaaaaag gatcagctcc
     2521 tgcacgacca aaagctacaa acgataaaac agccccatca cgcagaagcc gctcaagagt
     2581 ctaggaggaa gaaaaatggc acgaacacag aaacaaaaag caactcaaac tcatgcagtc
     2641 agtttgatca cattaacaaa cccatcttca ccaatcgccg aacaatatcg tacgatccgg
     2701 acgaatatcc agtttgcctc cgcagctggt cagcaaatca aaacgattgt cgtgacttca
     2761 tcaggtcctg gagaaggaaa atccaccact gcagcaaaca ttgccgttgt ttttgccaaa
     2821 tctggtcaac gggtactgct agtcgatgca gacttacgta agcccgtcat ctataaaacg
     2881 ttcaagttga acaacgcaag cggattaagt actgttctta gttcatcagg aagtgtagca
     2941 gatgagatcc aacgaacacc agtcgacaat ttgtcgattc tgccaagtgg acctaaacca
     3001 ccaaatccat cagaactact aagttcgcca agaatggatc agattttagc agaagcaaga
     3061 caactatttg atgtagtgat tttcgacatg ccaccagtcg ttgccgtaac agatgcacag
     3121 atcatgtctt caaaaacgga tggcacctta ttggtcgtac gggaaaatac gtcacgaaaa
     3181 gaatcattga ctaaagcaaa agaactgtta gatatggtac aagcgcgagt ccttggtgtt
     3241 gtatacaatg gagcagaaca cagcagagat gctggctatt actacgggaa ttaagaaata
     3301 aaaagagagg agacaagaga aatgatcgat ctacattgcc atatcctgcc aggaatcgat
     3361 gacggagcag aaaacataga tgccagtatc gccatggctg aaaaagcgat tcaacaaggg
     3421 atcacacata ttttatgcac accccatcac aataatggaa aatacagcaa tgaaaaatca
     3481 caagtcattt ctctagtcgc ttcgttacaa gcagaattag agaaaagaca attgcctttg
     3541 acgttattgg aaggacaaga agtacgtatt accggaacac tgattgaaga catccaccga
     3601 gatgaaattt tatttacaga tttagatgat acatatcttc taatcgaatt tccgacactg
     3661 gaagtgccgt tatatgcaga atggctcttt tttgagttgt tacaactagg aaagacacca
     3721 gtgatcgtcc atccagaacg caacgcgcat tttcgagaag acccaaatca tttgctgcca
     3781 ttcctagata tgggatgctt ggctcaactc accgcaccaa gttatgtcgg tgtatttgga
     3841 aaagacatcc agaaaacagc caaaatgatg gtcaaacata acctcgtgca aatggctgct
     3901 tccgatgccc atggggtctc taaacgtacc ttttacttaa aagaatgtta tgagcaaatc
     3961 gccaaggact ttggtaagga aaaagtagca cagatgaaac aagtcgcaaa ggacctgatc
     4021 aacggggatg agatcagtta tccagtctat gaagaggtaa aaaagaagaa gtttggttta
     4081 ttttaggggg aatgtagggt gtttgtatta acaagaagaa gaaaaattgc cacattggta
     4141 tttgtggaca gcttgttgtt gattgctgcg aatattgcag cagctaaatt catgaagccg
     4201 tttgtagcta taccaatgga ccttatttta atctctatcg gtttgtcgat tggtttttat
     4261 ttattctatg gtagtttatt caaagttttt actcgaatca atcgttatac aaatttaaga
     4321 gagatcattg cgatttttgg ttcgttatct gcaagtgcgg cctcaagtat catcatattg
     4381 cttttcataa ataaacaata cagtttacgt ttagtgatct ttgcatacct actttcgcta
     4441 ttactgatca ttggcagccg attgatctgg cgtatctata tcgaaacaaa aaatatgagg
     4501 tatctatcaa cagattccgc taaaaatacc ttgatcgttg gggccggcga aggtggacgt
     4561 attttataca acagctttct tggttctaaa acggctcaag atatccatgt cattggattc
     4621 gtggatgatg atccgaataa aagaaatacc tatctttcag gaaaaaaagt actggggtca
     4681 ttaaaagata taccggaatt gatcgaaaaa tatgacatcc aaatggtaac gatcgctatc
     4741 ccttctttat caagaaaaaa attacgaaga atcttcgagt tagtcgaatc tgctcatgtg
     4801 aaagtcaata cgatgccttc tatggaagaa cttgcctcag ggaaaatcag cgtatcaaaa
     4861 ttaaaaacca tcgacgtagt ggaattactg ggaagagatg aggtagagtt ggatatcgaa
     4921 tccatcaaag accagatcac aaacaaagtc attcttgtca ccggtgcagg aggatcaatc
     4981 ggttcagaaa tctgccggca aatcatccaa tttgaccctg caaaactatt acttttaggt
     5041 catggggaga actcgattta cttgattgac cgtgaattac gcacgcatca tcaaaagtgt
     5101 cctacagaaa tcgtgccgat cattgcggat atccaagatc gcaagaaaat caatgagatc
     5161 atggaacaat accacccaga tatcgtctac catgcagccg ctcataagca tgtaccattg
     5221 atggaatata acccaaaaga agcagtcaaa aacaatattt tcggtacaaa aaatgtagca
     5281 gaagcagcca aagcagcgaa agtaaaaaac ttcgtcatgg tctcgacaga taaagccaac
     5341 aatccgccaa acgtcatggg ttcaacaaaa cgaatcgctg aaatgatcgt gactgggttg
     5401 aatgaagaag gatgtacgaa attctcagct gtcagatttg ggaatgtcct tggttctcgc
     5461 ggtagtgtga ttccagtatt ccgggaacaa attgctcaag gagggccagt cactgtaaca
     5521 gactttcgta tgacccgata tttcatgacg attcctgaag caagtcgttt agttattcaa
     5581 tctggggcgt tagcaaaagg cggcgaaatc tttattttag atatgagtga accagtgaaa
     5641 atcgtagatc tagcaaaaaa catgatccga ttgagcggat atagcgaaga cgagatcgaa
     5701 atcattgaaa caggtatccg tccaggggaa aaactatatg aagaactatt gttagataag
     5761 gaaagaaacg atgaagcagt ttacgagaag atctttgtgg gaaacatcaa aggttattcc
     5821 atgcaaacag tgatggaatt tgtcaagagc ctgccgcaag acgatgaaca attggctaaa
     5881 gacattgtca cttttgcgaa tgcttcgaat aagtaggggg agtaaagtga aagcaattat
     5941 tgtaggagct ggtggatttg gcaaagagat tgcttttcta ctagaaagtt tctctagata
     6001 tgaattgatt ggttttgtcg atgattcgtt aaaaatgcaa aatcaagaat tagtgggaaa
     6061 aactgtattg ggaacaatag actcgttatt agaattagaa gaagaaactg ctatttttct
     6121 gggaatcgct tcgccagata tcaaagagaa aatatatcaa aagataaata aaaataataa
     6181 attgattttc cctaatttag ttgcatcaag tgcactagtg ggcataaatg ttcagttagg
     6241 aattggcaat attttgatgc cctatacgac ttacactgct gacgtagttt taggaaattt
     6301 caatatgatc aatatcggct ctactatcgg acatgatact caaatcggta attataattc
     6361 aatttttcca agtgtaaata tttcaggaca tgttctatta ggagataaaa a
//
LOCUS       DASJTC010000120         6354 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.123, whole genome shotgun sequence.
ACCESSION   DASJTC010000120 DASJTC010000000
VERSION     DASJTC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.123"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(99..1229)
                     /locus_tag="U4B19_002522"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="U4B19_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="U4B19_002523"
     CDS             complement(1878..3080)
                     /locus_tag="U4B19_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2078195.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="U4B19_002524"
     CDS             complement(3140..3343)
                     /locus_tag="U4B19_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEN2078196.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="U4B19_002525"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="U4B19_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="U4B19_002526"
     CDS             4880..5692
                     /locus_tag="U4B19_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2078197.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1898 a         1301 c         1003 g         2152 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtg
//
LOCUS       DASJTC010000121         6315 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.124, whole genome shotgun sequence.
ACCESSION   DASJTC010000121 DASJTC010000000
VERSION     DASJTC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.124"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            367..2004
                     /locus_tag="U4B19_002527"
     CDS             367..2004
                     /locus_tag="U4B19_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078198.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2149..2889)
                     /gene="truA"
                     /locus_tag="U4B19_002528"
     CDS             complement(2149..2889)
                     /gene="truA"
                     /locus_tag="U4B19_002528"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEN2078199.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3080..3715)
                     /locus_tag="U4B19_002529"
     CDS             complement(3080..3715)
                     /locus_tag="U4B19_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEN2078200.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3862..5103)
                     /gene="sstT"
                     /locus_tag="U4B19_002530"
     CDS             complement(3862..5103)
                     /gene="sstT"
                     /locus_tag="U4B19_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEN2078201.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            5301..6299
                     /locus_tag="U4B19_002531"
     CDS             5301..6299
                     /locus_tag="U4B19_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078202.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
BASE COUNT         2058 a         1159 c          946 g         2152 t
ORIGIN      
        1 agaaaaggat aaaaatagtt cagtccatct ccataattgt taaatgttgt atagttgatt
       61 ggcccttcaa aaatagaaga taaccctttg attcgtgaaa gatgaaaaga taaatcacct
      121 gttgtataaa tttcttgagc atttctcata aaatttactg aatataaagt actaagtaag
      181 ataagaacta ctaaaagaaa tgctttttta cttattttca tagatttgtt gttctccttt
      241 tttaattcta ttttcagtat actagaattg tcttttttta ataagttttg cgttaaattt
      301 ttcatagcac tagtatgata tactagaaaa gaatcaattt ttattacttt taaataggag
      361 aaaaaaatga aaaaaaacaa aaaccaaata atagatatca tttttatgtt atttctaagt
      421 tttgcttacg tcatccctct attcatgtct aagggaatct atcatagtgt caatcaggat
      481 acttactttc atttatctcg aatcatagga ctagataatg tatggagttc tccagtaaac
      541 ttcaataact tcgatcatca tggaacaatg atgaatattt tttatccatg gcttactctt
      601 tatccagctt ttttatttta taaaatgatg ggaaatcttg ttttaagtta taatatctac
      661 tattttttta tcactttttt aacaatggtc gtttcttatt tttctatgaa acaaataaaa
      721 aacaatcgtt atatttcgct gttattttcg attatttata cattttccgc ctatcgtgcc
      781 attgacatat tccatcgtgc ttcattggga gaagctgtcg ctttaacatt tttgcctttg
      841 atactaatgg ggtgttacga aatttatatt agagattacc aaaaatggta ctggttgtct
      901 attggcatga cgctagtagt gtatactcat ttactttctg tcgctatggt ttctgtattt
      961 attggaggaa cgttgtttct ttctttttat ttttgggacc aaaaaatcgc tagactctta
     1021 agtcttttaa aagctaccgt cctcaccttt tttttaagtg cgggattctt aattcctttt
     1081 attcaacaat ctagagcaca agaattgaaa gtcccactgg gaaaagaatt aagtggtatg
     1141 gctcctagtg atatgctgac acatatttta aataataact ataacaacta tactattgga
     1201 ttattcttat ttctcggatt gattggtgct tttattttta taaaaaaatt aactgctgat
     1261 gatttattta ttttctttct tggtgtattt gttctttttt gttcaaccaa tttatttcct
     1321 tggcaacttt ttaaccatac accagttaag agtctacaat ttgtctggcg gttaaatggt
     1381 ttttcttctt tatttatagc ctatacgatg agtatagtga tctattatat tttttctact
     1441 aaaaataatt catggaaaat aatggtcttt acgtttcttg cactaatatt gcatctctca
     1501 ggagtaagca attctatcca tgctaacaaa gattcattaa cacttatagc acctgaagac
     1561 gctgttgcta tagctgaaaa ttacaatcat actgattatg caaacaaaga gtcaatctat
     1621 catcctgata tgattaataa tgatcaatat ttactaggta accaggaaat aaatccaaca
     1681 actcatttta tgagtaacaa acttacaatt gaattagata attcgaataa taaaaatact
     1741 gtcttgacga cacctattta tcgttataaa gggcaagtgg cttctattaa tggcaaacta
     1801 gtacaaacaa aattatctaa gtttggcaca actgaattaa ctattcctcc aggtataaat
     1861 aaagtcgtta ttacttatca gtacacaaaa ctagcaattg cttcaagata tttatctatc
     1921 gttactttga ttttattttt actttatcga tttacttttt ctaaacaaaa acaaccgaga
     1981 agagaagtac aacattctca ctagattttt atttaagaaa gactttttag ctgtacacat
     2041 aaacaggcta aaaagtcttt ttttatattt ctttaatttg cttcatacat tatttaacat
     2101 cgatatttat actgtgaaac atgcgtttca tccattatca aagtattttt atcgataata
     2161 aacttcatcc aaaaacagac cttgtgcagg gacggtttct cctgcatgtt gccgcacttt
     2221 gctttcaaat atttcatcga ttgtttctat cggcatggca cctgtgccga tttctagtaa
     2281 tgtccccatg ataatccgca ccattttata taagaatccg tctccgacaa aagtaaaatg
     2341 aagcatttct ccctttcgtt cgatagataa ttcatcaatc gtacgcactg tggatttttt
     2401 tgattttttc aatgcagaaa aaccaatgaa atcatgtgta cccactaatt tttcgcaagc
     2461 tgcattcagc ttgtccatat ccaactcttg agggaaataa aagctgtggt ttcgctcaaa
     2521 tgctgaagga atcacgttgt tccaaacata atagctgtat tttttgccca ccgcattata
     2581 tcttgaatgg aagcgctcag gcatttcttc gattcttttg atgacaatat cccgcggaag
     2641 gtaacgattc ataaagtcga tcatttctgc cagtccaatt tctgtgtttg ttttaaaatt
     2701 tgctacttgt cctcgcgcat gggtgccagc atctgttctt cctgatccaa taatttctat
     2761 ctcttcattt gtcatctgat gcaatacttt ttcgattttt ccttgaatgg ttttatcgtc
     2821 atctccaagt ctttgccatc ctgaatatct ttttccgtca tattcgattg tcaacttgat
     2881 gtttctcata ttttagtggc tcctctgtct tccatttggt cttctctcta cctttttaag
     2941 attgtagcac aaaatatagc tccttgcttc tctaagacag aagctataca agaaaactgt
     3001 ggaaatatat atttcagaaa aacaaaaaac agccaccgga tgaagtatcc gctgactgtt
     3061 caatttgacg gctcaagttt tactgtttga gatgcaagat actcaaccag gcaatgggca
     3121 cataattaaa gaaaaaactg attctttggc aaaaacctat tttattcagt aatggccaag
     3181 aatttataga aggaattctt gccagtccat aaatcgccgc aaaaagcaaa ctgatcacag
     3241 acagtccaag gtataagtgg cttacgtcca tttgcccatt ttttcgatag atcaagaaaa
     3301 gaagaactgg aaacaataaa aaaccactgt aacctaaacc tgatccgata ttatggaccc
     3361 atgtcgagac attccacgaa gattcatggg tattgatact gaacaaacct gtaaagatac
     3421 aatcacctat accatagaac gcgatggctg ctgtaactag ggtagctaac acacgagatg
     3481 tactaacaaa tgcagcatac aatgcaggca atgacaggat gaaaaagatc cctgaaacca
     3541 cagaccaaat aagaaacgct tttcttacag gactggaaac ctctccaaat acactgatca
     3601 cgctcttcat ttgatctaat tcaggataat acaatcctaa tacgtacgga gtcaaaaaat
     3661 cactcagcac gcctaataat aaaaagaaaa aaccaaattt acgtaaaaaa tccatgatac
     3721 gcctccaata acaattacct atgcttttaa ttgttattat aaacgacttt atcttgattt
     3781 aacagaaacc tctatttaca cgaacatcta tataactcat taaaaactcg aatcatttta
     3841 aaaatactaa atatctaacg ttcaacgttc acttaatcca tctaaacaat ctttcaattt
     3901 gattttttcc ccacttcttt tacgcgcgcc tagctctcct attgcagtaa acaacagatc
     3961 acttgacgaa tttaaggcag tttccattga atcctggata acaccgataa taaatccaat
     4021 accgacgatt tgcatcgcga catcattaga gataccaaac aaactacatg ctaatggaat
     4081 caataacagt gaaccgcccg caacacctga agctccacaa gcagctaaag cagacaaaat
     4141 acataaaagg actttcaaaa caaaaggtgc ttcaatccct aaagaatgta cagcagtcaa
     4201 cgtcatcaag gaaatggtaa tcgcggcacc gcccatattg atcgtagcac ctaacgggat
     4261 cgatatggaa taagactctt ttgtcaattt caatctttca gccaacatca tgtttatcgg
     4321 gatatttgcg gcagaacttc gggtaaagaa agcgggaagt gcgctttctt tcaaacaaaa
     4381 gaaaatcaat ggatagggat tttctttaat aaacaaaaac gtgaggaatg ggtacacaac
     4441 taatgcgaca aacagcatcg taccgatcaa aacaagtaaa agctgcagat actctgccaa
     4501 tccagcgatt ccagtcgtag caattgattg ataaaccaat cctaaaatcc ctatcggagc
     4561 aaatgcaata atcacttgta cgacttgtga caacgctata gatatctgat ccaccaactc
     4621 ttttgtcgat tctgaacgtg ctcgaaaagc tagtcccaac aatacagccc agaataagac
     4681 gccaaggtaa ttcgcatcaa taagagattg aacaggattt tgtgtgacat tcgtcaacat
     4741 agtagataaa acactttcca aactttgcgg tgctttttca gatactgttt cttgtaaagt
     4801 aataggaaca gtaaaaattg aacttacaat aactgctaca agtgcagaaa gcaatgttcc
     4861 cactaaatat aagatcaaaa taggcttcac gaatgtctta ttcccaattt tatgcttggc
     4921 aattgacgcc atcgtcaaga aaaaaaccag aataggtgcg attgccttca agcaactgac
     4981 aaacaacgtt cccaatattg aaataaatgt ccatgtgggg aaaaagatac caaacaatat
     5041 tccgactaaa ataccaacaa caatacgttg aactaacgac atatccaaaa acttctctaa
     5101 caaatgacca ccctttcaca attttaacaa tcattgtatc attttatgca aagaaactaa
     5161 aggtataatt ttctaaaaat tctaacaata caaacaacca tttcttgcta attttgtact
     5221 ttttctgtct acacgttgtt ttttgagata ttctaagtta ggatgttatt aatgggcaac
     5281 ttatcaggaa acgttttctt atgtttttgt ccaatttttt tgtgaggtgt ttattcttga
     5341 aaaaaataaa gaaggagagt tatggcttcc tgacaatctt tgtcgcttgt ataatgatgg
     5401 ttttacagcg tatattgatt tcagcagtca ctattcctac atatgcaaca aaccctggtc
     5461 gattctacat ctatacattt ttacaaactt ccgttgttat tggtgcaaat ttgatcccac
     5521 tttacatggg ttaccaatat caaaaaatcg cccgtttaaa agttttacat tatttaagtc
     5581 gttttgcctt tatattccta atagccacac tagtttgcaa tatctttttt tacgtacaag
     5641 ctggcagctt gaatattcgt gattattggc ttatcctaac acctatcagt cagaattact
     5701 ttacttatgc tgtttcttgt actttgcttt tttgtagtat tccttataca gttggatttt
     5761 taaataaatt atcgaaaaca acaattaaat atctactatt atttttaaca attggattag
     5821 ttggctgctc tactctattt aataaagacc cgtttgcatt acagaatgga cagagtattt
     5881 tatggatttt ttatttattt ttaatgggtt atggtttgaa agaatggaac tggaaagaaa
     5941 aggtccgcta taagcttcct caagtattta tcagcttgat tgttttattc ggtttgatta
     6001 ttttgatgac acaaatttca ctcatcatca gaggaaatac agatacagcg ctgaggtttt
     6061 ctaaaccata ttctctcttc gcaatgtatt atactttctc ttcgttttta tttttagaaa
     6121 tgctatctga taaattaggt ataaaaggaa gaggaaattt tcttgctgta tttttgattg
     6181 tcactcaagt tatatgtaat ttccctttag tttcttatcg cgtatctaca tttatgaaac
     6241 gtgaatttcc aaactcaggg aaggcatggc tgataaatat cgggcagttt tttatttgat
     6301 atttattagc agtgt
//
LOCUS       DASJTC010000122         6173 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.125, whole genome shotgun sequence.
ACCESSION   DASJTC010000122 DASJTC010000000
VERSION     DASJTC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.125"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            725..811
                     /locus_tag="U4B19_002532"
     CDS             725..811
                     /locus_tag="U4B19_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN2078203.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="U4B19_002533"
     CDS             complement(932..1621)
                     /locus_tag="U4B19_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078204.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="U4B19_002534"
     CDS             complement(1866..2780)
                     /locus_tag="U4B19_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN2078205.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="U4B19_002535"
     CDS             complement(2762..3142)
                     /locus_tag="U4B19_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEN2078206.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="U4B19_002536"
     CDS             complement(3592..3879)
                     /locus_tag="U4B19_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN2078207.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="U4B19_002537"
     CDS             complement(3899..4123)
                     /locus_tag="U4B19_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEN2078208.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="U4B19_002538"
     CDS             complement(4578..4748)
                     /locus_tag="U4B19_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078209.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="U4B19_002539"
     CDS             complement(4763..5296)
                     /locus_tag="U4B19_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN2078210.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="U4B19_002540"
     CDS             complement(5317..6159)
                     /locus_tag="U4B19_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2078211.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DASJTC010000123         6152 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.126, whole genome shotgun sequence.
ACCESSION   DASJTC010000123 DASJTC010000000
VERSION     DASJTC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.126"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(640..1143)
                     /locus_tag="U4B19_002541"
     CDS             complement(640..1143)
                     /locus_tag="U4B19_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEN2078212.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1502..2527
                     /locus_tag="U4B19_002542"
     CDS             1502..2527
                     /locus_tag="U4B19_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEN2078213.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2850..3728
                     /locus_tag="U4B19_002543"
                     /pseudo
     CDS             2850..3728
                     /locus_tag="U4B19_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
     gene            4204..4320
                     /locus_tag="U4B19_002544"
     CDS             4204..4320
                     /locus_tag="U4B19_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEN2078214.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5248..5586
                     /locus_tag="U4B19_002545"
     CDS             5248..5586
                     /locus_tag="U4B19_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078215.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5680..5910
                     /locus_tag="U4B19_002546"
     CDS             5680..5910
                     /locus_tag="U4B19_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078216.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         2098 a          910 c         1118 g         2026 t
ORIGIN      
        1 aaattagtca aagaagggcg tatagatttc ttttgatagg agatatacaa cagaaatgaa
       61 taaatattaa cgtgttttca aacagtataa aacggtgtgg tttttggaat aaaaaaataa
      121 catatctcat tttacacttt tgttatttta tacttaaaaa ataacgacac agaaaatacc
      181 gtgtctcaga tttctctact acttctaaaa actactgttt ttttcctttg aaatgccttc
      241 aaaaagtagg atcaggcaga atatactggc agaatcaagg agaagggagc ctaaataagg
      301 agaaaaacca tagaaaatat aaagtaatta cttataaaac aagcttttta tagaaaaata
      361 atatacctga gtagggtata aattaagacg gaattccttt ctagattgag atattgacta
      421 ggaagaatgt taagatttta agcctttaag ctaaaaaaaa accctttttg ttaaataagg
      481 ggaatatagc ctgtcagttt caaactgcaa gtcgtcctgc tgcaataata tagtagatta
      541 gagatgaaaa tagatttttt taaaccaatg actgaataaa aagaccgtca tagatacaca
      601 ttggaatgtg tctattacgg tcttttttta cgaacgattt tattgaagac ggacagttcg
      661 ggtcaaaaat aaaaaagcta cgatcagaaa taagaataaa atgtatccca aaataatggc
      721 tagacggtct aacttatttg tcgatagatg tttgtttaga agttgatagg cccataaacc
      781 tatcagtcct cctgttgtat tagttatcaa atcagtgatg tccgctacgc caataccgaa
      841 taggaattga atcaattctg ccaagaagct tacaaggaaa ataatcccta attttttaga
      901 aaaagaaagt ctcttgaaat tgacattcaa aagcacccca aaaggaaaaa agacaatcaa
      961 gttataagtg gtctcacgta ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac
     1021 gagattgaca ttgctgtaag aataagtgag aaattgtgga agattaaatg atagcttgaa
     1081 taaaatcaac cataatagaa agaataaata aatccctaat aaaaattttg atacataccg
     1141 catagacatc tcctccattt aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa
     1201 agtatgaagt ttaaggacca acgtagttat cgtacgaaaa ttagaagctt ttttttgttg
     1261 ggaatccggt ttctttaaca aagctaagat agcgagttgc ttggaaaagt tgctatcttt
     1321 tttctgtcaa aacgactcgg aatgttcgtt ttttgttcta atttctccaa gttgtttact
     1381 ttttgatact aagtttatga aaaatcaaga aatagttgtt atactaggta atgctgacaa
     1441 acgggattgg catttcatct tggttcagct ttttttccaa ttactaggaa ggagggatag
     1501 attgttcaag acttattttc gtttcaataa aatacaagat aatccttttc gtatcatgaa
     1561 tctgatgcta cttatcttta ttgtattatg gttaggttac ataaatcatg attttttgat
     1621 ttcaagtttt tcatcattag ggatctttac ttttttctat tatcaaaaca taccgatgaa
     1681 atatttgctg aaacgtatgt ttctgatcgg ttgcggactg ttggttgctt tcgttttggg
     1741 gatcttatcg acttatgttt tatggcttga accatttgcc gtagctttgg ttgctttttc
     1801 cagcagattc gtgttgcgat tattccatat ttctaaacca ggtggcttgt tttttgcgat
     1861 gctttctgcc atgggaacga gcatgcagct gccaatcgct caattaccga tcgtctcact
     1921 ttattttttt atgggtgtcg tttttgccct gattgcggca gtgattacaa aattactgga
     1981 ttcacggcct gagcagacaa ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc
     2041 tttagtgatc attgattctg ttttctacag cgctgcctta tttctttccg tttatgtcag
     2101 tcacggcttg aatctgcata atccttattg gttgaccttg tcatgtgcat cgattttgct
     2161 agcagaaaac ttggatgcga tgaagcatcg acaagtccaa tatctgattg gatcgatggg
     2221 tgggttatgt gtgtcggcat tcttgtcatt tgtgccgttt acgcagttgc agaccatttt
     2281 tctgatcact tttctatatg gaatcgcaca atttctagtt gctagaaatt atgcagttgc
     2341 taatattttt cttaatccga tggcgttgat gctatctaca cttattcgtg gagcatactt
     2401 gatttctttg atcgaatatc gttttttagg gatcgtcata ggaagtttca tcggtttagg
     2461 tgttgcgtgg gtaatgacgg ttggtttgca acattattta acggttgttc gtcaaaaatt
     2521 agaatagaag gacaaatata tgtgccaaaa gaggttatga caagattttt gtcacaacct
     2581 ctaattcaag gtgttcaaaa gccagattct cagcattaag tcaaattcac acaaaaatgt
     2641 ggaaaggaac tttcaaaaaa attttatatt tattctatca aaaaattttg tttatgatgg
     2701 taactgaata aactttcctg agatggtcaa tgctttcctt gaaagtctta cagatattct
     2761 ttcttcatag aaagtttaag ttttatagtt agtgtacaat aaaagtatgt ttataccata
     2821 tggtgtccgg ataatcagtc atttaaacaa tgaggttcaa agatatgaaa aggggaaatt
     2881 tgatgaagaa aataagcaat gaaatccaaa ttctggcaga aactttaact cttcccaaag
     2941 tcgttataga gcaaatgatt gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat
     3001 atagtcatga actaatggat cctaataaag caaaggcgac tgcaaagaaa atagacaaag
     3061 tctatgaaga ggaacctttt gcagcattag ctatttatct atatgcagct tctcaaagtt
     3121 ggaaaaaaat ttatcaacca ttgaagattc caagaactat ctatttggca acaatgaatg
     3181 cttttactag atttattcat gaacatttcc aagtaacagg aagctatcgc tttgaccgtg
     3241 gtttttggat ttggcattac actagtggct tgatctttcg gattggtgaa ttggagtacg
     3301 aaatgacata ttttccgaaa gggtacacgg atctaagatt agaaggaaag cagtgccttt
     3361 ctcttcatat tccttccgat gcagatttat ctcagaataa gataagagat agttacgaaa
     3421 gtgccattcg tttttttgaa ctttattttc ctgattacca ttaccaagtg atgtacacag
     3481 atacttggtt actatcaccc aatcttacta aatagttgaa aaaagagtca aaaatctgtc
     3541 tttttgcagc cgattatcga ttattatcag ttgatgaaca ggacgatagt ggagtccctt
     3601 ggatctttgg acgagtagat gcccaaatac atgattatcc agaaagtacg agtttacagc
     3661 gacaagcaaa agagcaattg cttgcaggcg agcacattgg ctctggctta ggtattctta
     3721 cttcataagt ttagtacttc gaaatagttt aaaagagcgt gagacaaaag taaagaacac
     3781 ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc cttcggaaat
     3841 aagctgaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga
     3901 gttaaacgct tctgtcccag cctctttttt agtgttctat cgaacacaca gtacgttcaa
     3961 taggtaaact agctataaca atagatatta aagttaacgg acaagagggc cttttttagg
     4021 tagctaaaac gtaataatga aaatttattt ccagagatag gagtcaatca gctagtctga
     4081 tcgactgttt taacaagttg actttcattt taacttttga taccatttaa aacacaaatc
     4141 gtaattgttt cgatttgtgt tttttagcat gactacaatc aaagaagaaa gtgggtcgtc
     4201 catatggaaa aaacagatat ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa
     4261 acacgaaaac gtgcgctgaa gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa
     4321 gaagaaggaa acttaattaa aaaaatagcc gaaaaacgtt tttcggctat tttcaataat
     4381 cggtttttgt gtaaggtttc cgaaagaact gctgttgatt ttccattttt aacatatgat
     4441 aaattggtag aatctttctt ttcaaattga taccattccc taattccagt tatatagtta
     4501 actacagaac cacaaagcac gtacgtttat aaaagtatta tctgtagtta tttttcaatt
     4561 ccctttcaac ctcttatttt gaccagtagt attgatcact ttacctttta acacctttgt
     4621 tcgtttttga aacattttta gtcaagaatg gaaacgtaac tacataatca aaggttttgc
     4681 gaaatatttc atgaaatatt tcatgtagaa gcgagcaata cggttgcaaa taaccattat
     4741 cgtttaataa gcagttacga aagctcccaa tatctctaac gttcagctca ctccatttct
     4801 tttcttcaaa aaaatcagtg attcgtatct ctttctattt gtcgaaaaag ttaactttaa
     4861 aaagtaacgc cgaaaaaata tgcaaaaaaa taaaaaagca gttactattg ctagaataac
     4921 atcaaggaat aataagtttt ggttttcaga taatagtcga taagttgtgt attggaattt
     4981 tagatacagt aataaagtat ttttcatgaa attttgtgag aatcactatt acaagtatct
     5041 ttaataaaat taatggttaa ggaggaactt aaagtagaat gtaaatgaat agttgtagtc
     5101 agtcgattgt gatactagac tcaaagttgc aggaataagt ttttcaattt gtgattggaa
     5161 gataaaaaat gaaaaagaag ggcttttttt tatactttgt tgattgtagt gtttattttc
     5221 cctctgttta gccaagtaac attttctatg actcaaccag tttcaagtga ggaacatgat
     5281 aacctttttc gtgctacaga agagtttaag aaatatcagg aaaatattgt tccggagact
     5341 ccattagcta gtgagccaat tactgatatt gatggaaacc aaataggatg gtatatacag
     5401 tacgaagtta tggtggtaaa taatgactca atagtaaaaa atacagtagg cttagagcta
     5461 acaccgattg aaaaggtgag ttcattgtta acgtatattt atactgatga aagtgacgag
     5521 actgagatca ttttaacgga ttactcaaaa attgctactg ataaaaagta tatattgtgg
     5581 atgtagacac tagtgaaata ctttatgata tagaaattga tgaaaattta ctagatgtag
     5641 cacaagaaat cgatcatcaa aagcaggaag ctattcgata tgctggtaga aaagggaaaa
     5701 gaaaatattt attacgtgaa tgtagcaaaa gttagagaag atgaaaatga gtggaaagaa
     5761 ttcaagagtc gctactcaat taactcgact ccgacattta cagtttacag agaaggatcg
     5821 atagaaaaaa ctgttttttg gactaaagaa agtggaatgt ctttagctga agtagaagag
     5881 tttttggatt atgttagtat gcaacagtaa gggagttctt attatgaaaa attttgacac
     5941 atattatgtt attgtttttt cgatatcttt gctgttaata ttactaactt taatgcagta
     6001 taaagagtat ccagagtatt ttattttaac ttttgtatat ttagtaagta gctacacact
     6061 tctatataaa ttcagaagtt cttctagagt ttctaaagtt tgtttcttaa ttagcttatt
     6121 gattctataa tatttttgat ttatatagga aa
//
LOCUS       DASJTC010000124         5825 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.127, whole genome shotgun sequence.
ACCESSION   DASJTC010000124 DASJTC010000000
VERSION     DASJTC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.127"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..613)
                     /locus_tag="U4B19_002547"
     CDS             complement(<1..613)
                     /locus_tag="U4B19_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2078217.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMD"
     gene            815..1114
                     /locus_tag="U4B19_002548"
     CDS             815..1114
                     /locus_tag="U4B19_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2078218.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            1379..2254
                     /locus_tag="U4B19_002549"
     CDS             1379..2254
                     /locus_tag="U4B19_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078219.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            2251..2469
                     /locus_tag="U4B19_002550"
     CDS             2251..2469
                     /locus_tag="U4B19_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078220.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            2456..5083
                     /locus_tag="U4B19_002551"
     CDS             2456..5083
                     /locus_tag="U4B19_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078221.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            5098..5316
                     /locus_tag="U4B19_002552"
     CDS             5098..5316
                     /locus_tag="U4B19_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078222.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
BASE COUNT         2221 a          736 c         1114 g         1754 t
ORIGIN      
        1 agtccatttc gtctaatagc tgttttaaag aatcaatcat tctttctttt tcaccgtatt
       61 tgaaatcacc atttcttatc ttgtcttgaa gtttactgtt ggctcttttg aaaaatagct
      121 ctttagagat agagtgtctt tggttagcgt actcttcaat cagaggattg atattattag
      181 caagtatctc atttagataa atttcaaaat cttttgagta atattttgta aatatatttt
      241 ttattatagg attcaataac tcatagtctt tatctagcga aaacgtatca ctatatcttt
      301 cttgaatgtc tttaaagact ttgtgaggta tttctgggaa gtccttaata tatagctcat
      361 accctatatc gataaggtat gaagttatat attcttctaa agttccccat ttaatccagt
      421 caattgttga attagtgata gttgataata atttcagctt ttgtggattt ggtaaattgg
      481 tcccacgttc ccagttggat atggtcgcac gtggtgaatt atctattttc cttccaagtt
      541 cttccatagt aaaaccacat gaaaggcgta attgtttgat tcgttttcct gcttctgaag
      601 gatttggctt catctttttt ctccttttta tttttaaaga tagtatatca tatttataaa
      661 ataaaagtat ttaaaagtat ttacttttta tctggagtat gatactttat tcttataaat
      721 acaaataaat acattttttt acgcaatttg tatttatttg tacttataaa ggtagaggag
      781 ttatagaata attaattaaa aaggagaggt ttttgtggaa gcaataaaat taatgatttc
      841 agaagaacag gaggaaatgt ttctaaagca gttttatcaa attgctacaa aagctattga
      901 aaaggctcaa gaaaatgcgg ggatgacaag ggaatatctc aatcaaaaag aaatggcgga
      961 atacttaaat gtgagtgaaa actttttaaa aaatgctgtg agaaatgagg gattgcccgt
     1021 agttgaatta ggggcaagaa aattttattc gaaaaaggca gttaatgagt ttatgtttgc
     1081 tcgaatgaaa agaggggaaa atattggaag atgatgaagt agaaattata aaaaaatgct
     1141 ttaaaaaaag aaaaaaagtg ttcccaaaag tttgcagact gggggaacac tcaataataa
     1201 agtgctgaga aaactttatt tatagctatt atatcaaaaa agaaggtgaa aaggtgtttt
     1261 cttctggaaa tgttgtagtt gacaaactta gagaaataaa ttttgacgga aatgtaatcc
     1321 acacacatgg tacgatcaat taagaaaaaa aacgaaaaaa ggtgttgaga agccctattt
     1381 gaatgcaata gttatcttag cagaaattac ctattggtat agacctaaaa aaatttttaa
     1441 cgatgatggt cagctacttg gttatcaaaa aaagtttctt gaagatattt tgcaaaaatc
     1501 atataagcaa ttaagcaaaa aaacaggatt atcagaacga gctataaaag atgcaattgt
     1561 gtacttagag aaaaagggga ttatcaaaag ggtttttaga aatatttatt atgagaaaag
     1621 agcagttaca ttatcaaatg ttatgtacat tgttttaaat gtacataaat tgatagaaat
     1681 tacttttcct actttggatt gcgaaaaaaa ggagtatatg ccgaaaattc gtcagagggg
     1741 tatcgaaaaa aagagatatg tctctttaaa aaaaggtaga ggtatgtaca aatttgtata
     1801 tacgtataca aagactactt cacagaacat atcaaagact actctcgtta tggaagaaga
     1861 ggatattaat ataaagagga tttacagtaa gattagtgtt ctttatgacg caaataacga
     1921 agatggtcga aaattatttc aagtgtatca ctatttgaga aacgctaatg tagatacaga
     1981 agatatcaga aaaatcattg aatatttatc tgaaaatact cagtatctta ttgctgagtg
     2041 tattataaaa caacatgaag catgcttttt tcaaagaaaa actgaagaag ggttatacga
     2101 ttatgtaaca tattttttga atggacttca gaaaagatat aaaagtcaat ttttagaaaa
     2161 tgtagagcct atagatcagt tcaaaaaagc tttcgaaatc aaagagttac ctgaaattac
     2221 tatgcataat tggttgaaag gagaagaatc atgattaaaa atcgcttaat agatcaaatg
     2281 tggtgtcttg atcaaaatac agatactctc gaattaatcg agagtatctg cttcaacaca
     2341 gtagttctcg atttaagagc tgaaaatgac aattgtgtaa ctcatatcat aattaatcaa
     2401 aaaatggctc agcagctctc tgagagccta agaaaatggg ctgaaggaga aaactatgaa
     2461 agcaattaaa gaaaaaatcg ttagtagaaa aataaactgg ttagcactca gtgtacttgt
     2521 tttaatgatt agcgttgttt ttacaagtaa tattgttgaa gctagcattt tcaattggtt
     2581 tgatgacaca aaaagtcaaa cagagttttt gaataattct ctttatagac cttacctaaa
     2641 aaaatacaat ggagatttcc ttagtcagtt cgggatttgg cttggatggg ctgtggtaaa
     2701 aggcatgttt actgtgactg attcgattca aaatatgatt ccagatgtgt tggatttgtt
     2761 caatttcatt gaaagtacag ggctaaataa tgtttatcaa tcagtcataa atacaattgt
     2821 cgttggatta atgatccttt cattgatgtt tgttggctac aagatgatta ctggaaaagg
     2881 aacaatagat ttaagatctg ttggaatgaa tatagtgatg tctgtagcgt taattctttt
     2941 aatgccaaca atgatttcta gcggtattca atttgccaaa atattttaca atgatgctac
     3001 tacgattaca aattcagatg acggagtcgc ttggtcattg ataaaacaag gagtaacaga
     3061 tctggcatat attaataaaa cagatcaata ctcaagcatt gataaaacgg aggatcgaaa
     3121 taagttaaca cgaaaaaatt ttcaacagac agatttaaca caagtgttga cggataaagt
     3181 gattgataaa ttggaaaaag aaaatccagc tgcggataat cttcgctatg aattagttga
     3241 aaattctaat aatgaatttg ttgccacaaa gttttctgat aacttcttat caacattttc
     3301 tgatagcttg aaatcaggtt attatcgata tcaagcaaat ctttggggga tttcgattgg
     3361 gttgacagcg ttggcaatcg cctatgtgtt cagcgcgttt gtgatcatta cagcaatatt
     3421 agaacttgca tttaaacgtg tgttaggtgt tcttgttttt gcgacagata ttgaaacagg
     3481 acaacgttca aaagttgttt tatccgatat tttacaatgt tacttaacag ttggatttca
     3541 agggtttgga ctatctatgt ttgctatgtt tatcaatttt ttaaatgctg gacaaggtat
     3601 ctccacaaat atttttataa aaacaattgc ttatatctgt gctgtgtttg tgctgataaa
     3661 aggcagtgga acagtcatga gatactttgg tgttgatatt ggactcaaag aaggctatgg
     3721 gcaacttgct tctgcttttg gtatgggcgc aatgctgttt agaaaaggat caactggatt
     3781 taatcgtgct aaaggaagcg gaaatggaaa tggatcagac agtggagaag gagaagatcg
     3841 aaaaccagaa aagaatttcg gcgaaactct atcaaaaaaa gctggcaaaa ctggaagagc
     3901 tttgggatat gcccatgaac gtggattatc aggtttagct tcagatggag ctacaatggc
     3961 tagtgaaaga gcaataaaac catttaagag tatgcgtgat atggctaatg ataccaagaa
     4021 taagttcaaa gaaggtttag atgatggtac agtgtccgct attaataaaa atagcaaacc
     4081 tatgttagct aaaaataaag aagatgaaac tggaaaatat gctgattcca tgccaacacg
     4141 tctttcggat agaaaagatg gtgctaaggt tgaaaatgcc gatagaatta tgtctagttc
     4201 tgaacgtatg cgtgaagcaa tgaaaaataa tgctgaatcg aataataatc ctgtaagtgc
     4261 aattcagcaa aaagttcaac aagatattga agaacgtaaa aatgctatgc acggtcaagc
     4321 taagtctgct gaagaattaa ttaatcagaa gcgacaagaa gctaaataca cgcctgaagc
     4381 aatgaatcgt gaggaaatgt taaggaaacg tgtagaaggg cgtacaggag ctaatacgga
     4441 cgaaaaagaa gcgttgacca aagaaagaat ccaagaagct aaaaactcaa ataccggatt
     4501 agaaaaattg gtcaaagaaa atctacaaaa ttctacaagt agtcaaggtg gccgatcagt
     4561 agatgtccga gaaaatattc aaggttcatt gaatggtcat gtcggacgga atgttgatgt
     4621 aagagagaat ttacaaagct cttcaaatgg tcaaggtact aagacaattg atgttcgaga
     4681 aaatgtacaa aattctacaa gtagtcaagg gtttaaatca atggatgtaa gagagaatgt
     4741 acaaaattct acaagtagtc aagggtttaa atcaatggat gtaagagaga atgtacaaag
     4801 ttctacaagt agtcaaggat tcaaaacagt agatgttcgg gagaacgttc aaaaagatag
     4861 tggagaaatg aaagaaaaaa cacaaaaaat caatatcgtt gaagacagaa agtcagcgaa
     4921 aaaatttgat tcaaatcatg aaacagtgat tattgatagt gaaatccgtg aaaatgataa
     4981 gggatcaaaa ccaagacgaa gatttaccta tgaagacaac gagttattca gagatacact
     5041 taatgatcct aatccgcttt tcgataatct attaaaaaaa taaaaggggg aattgtcatg
     5101 aaattaattc taaagattag tacgcatgat ttcttagaac ttgacgaatt atgtgaatca
     5161 gtaaagaacg caacaaatga aaaaatcacg gtcactaatt atattgaact gatgattcaa
     5221 gaagaattaa agatgaaaca tgagataaaa aacaacttga aatattgtta tctagaaaaa
     5281 agtgaagaga taatccatga attaaatggc ttttaaaaat tgtatttgac atgaatttac
     5341 gacaaggaat tagtggggat taagcgatta ttctcttcca ctttagagaa ataatgggtt
     5401 aaaaagccag aatacgtaga gcaatattga caacctataa aggagctatt gttgaaaatt
     5461 gaagtaacaa aaagataaag ggaggtttac atctatcaga ttaaaagtga tagtacaccc
     5521 atgaaacctg caaaagagtt gaacctaatg tatatcggtt taactctttt tatttgcaaa
     5581 ttaaaaaaat atcaccgaag tgacgaatga aactatgacc aaattaagca agaaaatata
     5641 tcttgcaaat aaagtagtat aaaaagatat tgataaagcc aatcaaaact ttgaatttca
     5701 aacgttagat aatcaaacag atacaaaaaa tgaaatcagt tctacgcgta cgattattaa
     5761 aaataataaa ttttcactag aagaaaatgg aaataaaatc aaataattag gaagaaaaaa
     5821 caaag
//
LOCUS       DASJTC010000125         5779 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.128, whole genome shotgun sequence.
ACCESSION   DASJTC010000125 DASJTC010000000
VERSION     DASJTC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.128"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..813
                     /locus_tag="U4B19_002553"
     CDS             <1..813
                     /locus_tag="U4B19_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEN2078223.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSLTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKIARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="U4B19_002554"
     CDS             807..1229
                     /locus_tag="U4B19_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078224.1"
                     /translation="MVNKLIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            2363..3331
                     /locus_tag="U4B19_002555"
     CDS             2363..3331
                     /locus_tag="U4B19_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEN2078225.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKENLLDNLQNLFKE"
     gene            3341..3829
                     /locus_tag="U4B19_002556"
     CDS             3341..3829
                     /locus_tag="U4B19_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEN2078226.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            4125..4406
                     /locus_tag="U4B19_002557"
     CDS             4125..4406
                     /locus_tag="U4B19_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078227.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            4408..4824
                     /locus_tag="U4B19_002558"
     CDS             4408..4824
                     /locus_tag="U4B19_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078228.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
     gene            5438..>5779
                     /locus_tag="U4B19_002559"
     CDS             5438..>5779
                     /locus_tag="U4B19_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEN2078229.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2155 a          983 c          943 g         1698 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatttcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcccttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tttctaaaaa acaaaatttt cctgcggtta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgcgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acgtgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaaatagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaact cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctcgaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacgtaggta tttggctagc aaatatagag cttgccaagc
     1441 cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca aacgatacgt
     1501 ccataggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat ttggctagca
     1561 atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt cgagtgagcg
     1621 aatccaaatt tgcaactctg aagctttgca aatgagtgtg ccggcttccg taaaagtcta
     1681 ggtaacatta gtcgatatat acatagggct ctaatttgcg ttttaaggcg ttttaaacgt
     1741 tctggtatgt aattatacca aagcgacttt aaaacgcctt aaaacgaatt ttagaggttc
     1801 tgtagaacgt tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaaaaac
     1861 aagataaggg attttatcta gacttgtctt aaaacgccgt agatgagcgc tcagagcttc
     1921 taaaaggact ccttacgaac tgtttttaag caaaggagcc tttaggcgac tgcgcagttt
     1981 aatgtgagag cgtagcgatc acacctttaa agcgaccgta gggagcagat tcccagttta
     2041 ggaaatttcg cgaacctgtt tggagaaaaa aagtaggaaa attttaggcg ggaaattttg
     2101 cccccctctt ttataatctc ttttataacc tctttttaaa cctctttacc gtcggctgta
     2161 tccttactct cccaaggtgt gcagaatctt ttaggggtct ttttgtctgt ttttaggggt
     2221 ctttttgtct gtttttaggg gtctttttgt ctgtttttag gggtcttttt gtctgttttt
     2281 aggggtcttt aattaggggg ttgaccccca ttagaaagct ctttataatt ttgttaagta
     2341 gttaatcttg aaggaggatt ttatggcaaa tgaaattgtg aaataccacc atgaattaaa
     2401 tacgattcct ttacgcaaat ttacgtcagt agaaatgaat ttattcttct ctataatatc
     2461 tagaatgcgt gatgtaggcg ataaaaaagt acaatttaca ttcgaacaat taaaagattt
     2521 aagcaattat aaagcaactg caaatgttcg attcatagat gatttagaaa caacgtatga
     2581 caaattaatg gatttacgtt ttggtagaag aagtgctgac ggtttgcaac gtgaacgatt
     2641 tgttttattt aatcaattta aaatcgatgg aaaagctgat attccttttg ctgaaataca
     2701 agtccatgag aaagccttac ccttacttaa taatcttgaa gaatgggttc gatattctct
     2761 ccaacaattc aatgaattag aaagcagtta ttccaaaacc atgtttagat tattaaaaca
     2821 atacagaacc aaaggaattg ccaatttttc aaaagaagac tttcatgaat tacttgatat
     2881 ccctaaaagt tatagagaaa gtgatataaa taaaaaaatt ataaagccta ttcgccaaga
     2941 actaaccgca atcttcaaag gtttgacaat ccaaaagaga tacggaaaag gacggggaaa
     3001 gcctgtcatt ggctatcaat ttacatttaa acctgagata aaaaaatcag acgactttaa
     3061 caaacatgct ccaaccaaag aagaacaaaa taaagctttg gaactagcta atcaacaggt
     3121 tcaatctgaa aaagaaccta cttcattaca agaaaaaatc aaagaacaaa aacgaaaatt
     3181 agaactttta caacagttag cagaattaga aagaaaacaa aataccccaa gtaagaaaat
     3241 taacaaacaa atagcagata acaattccga tattacccca gaatttaaag aaaacttact
     3301 agataatctc caaaacttat ttaaagaata aggggttttt atgacagata aaactatcaa
     3361 agaattagca gatattctag gtgtttcaaa agataaagtg aagtatcaac tgaggaaatt
     3421 acctagtaat tttacctaca aaaaaggaaa tattacctac ctgaaaaaag aagcagtaac
     3481 aacaattaca aaggttttat caggtaatga atcgggtaac ttacctagtg aattacccga
     3541 ttcattacct accgattacc tactcaagga gattgaaact aagaataatc atattgaaga
     3601 aaaagatagg caaattgaaa aacttcaaat agctttagaa acgcagcaaa aattactcga
     3661 ccagcaacaa caactcaccc tacaagccaa tcaacaaatc aaaaaacttc acgaacaact
     3721 ccagctaacc tatgcagaag aatcttcaga aaaagaaccc ctttctgtta ctgaagaaac
     3781 tcaaaaagga caaaacaagg agaaaaaatg gtggcaattc tggcaataac ccaacaattt
     3841 ttattcataa aaattgttgg gttactttct ttttttgctt ttacaagcat agcgacatga
     3901 tctcacgtcg ctatttttta aagagcataa ggaaacgcgg tatcactttc ttacgaaagg
     3961 tgctaggtag gctgtgccta ccacgtttct aagaaaaaaa tttgctatca ctttttcgtc
     4021 attgtggata gcttatgtat agtgtttcgg caaaaaacag ctatacaaat actatatttg
     4081 tatgatatac ataaattata cacaaatgcg aaaggaattc ttctatggac attttagttc
     4141 ggaatattga tccaaaagtt atttcagaaa tagatagacg ttgccaagaa ctcacaacaa
     4201 aaactggcaa aaaatggagc cgtaatgcgt acctaaaagt tctaatcgaa aacgattttg
     4261 accaggcact taggcattac aaaaaagatc aattcgattt attactcgaa aaatttatcg
     4321 atatacaagc aaaaaatact gaaacattga acgactatgt tcatacaaac aaccaactca
     4381 ttgaaacact tatcggagga atataacatg agaaaaactt ctgttcaaaa acacgttacc
     4441 ctttatccca gtcacattga cgttttagat tccatcatcg aaaaaaatcc agatatcaat
     4501 aactactcgg aagccattcg ttttctatgt atgagttatg aacctaatac taatacgaca
     4561 actaccagca accaagcaaa actaaatgcg atgggaaaag aaatttctat tatgtccgaa
     4621 atcattgcag aatttgctga tttatatcta aaagatactg gtgttcttat cggtacaaat
     4681 tctaaagtat atcaagaagt gaaagaaaaa attgaaaatc aactcaatcc ctcccgacga
     4741 aatcaaccca aaaatcgtca aaaagaaacc aatcaaacat tcacttctag aaattttgac
     4801 ccaacaaact ttctaaaaag atgaaaaaga aaccgaaaat tttttctatt tctcagtttc
     4861 ttttttaggg ggcagtcttc cagcaagatt tgcaagttac cggtaacttg cacaaaaaaa
     4921 gaaaaatccg cttgtgcgaa tccttctttt tgcattttgg ggcgctgccc caatctccaa
     4981 atatcggtta tttaatagcg tggtaactca aaaaaataga acgtactctg aaataaaaaa
     5041 agactgctct aacgagcagc caataaaaaa taatgagcgt ttattcttga aaaagcagcg
     5101 cgcttatgaa gcgctgccaa aaatttacca tattttatga aaaataacaa gtttctaaaa
     5161 aggaatcggt taagattcac aaagaatcgt aaacagacaa tagcggaaaa tcaagcttcc
     5221 gaacgtacct tttagctaga ccaatgaaaa atcacaaagt gagcggctgg atcccttggt
     5281 acaggcgtta cttggtagta tacacattat gttaactaac gctataaata acgattatgt
     5341 tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt gtatatttta
     5401 attataggtg tataccactt aaatttaaca caaaggcgtg attattttga aacccaaaaa
     5461 gaaaatttat ctaggtctcc tcttttcctt gatgattgga atttttagta taggaattac
     5521 aaatacctat gcttcagaaa attctaattt caatatcaac tcccaaccaa ttgaagaaca
     5581 agcaattaat tttgcgaaaa ctgattttga acactccata acttcaattc ttttaatgga
     5641 aaataacacc gaaactgata ttaactatac tcttggttcg ccatttaaaa tgctcgatgg
     5701 agaaaatacg gcaaatagtt ctattttctt tccagtattt tcagacgctg ataatacgat
     5761 taaatatatt tataccgtc
//
LOCUS       DASJTC010000126         5727 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.130, whole genome shotgun sequence.
ACCESSION   DASJTC010000126 DASJTC010000000
VERSION     DASJTC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.130"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            196..921
                     /locus_tag="U4B19_002560"
     CDS             196..921
                     /locus_tag="U4B19_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078230.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="U4B19_002561"
     CDS             911..1420
                     /locus_tag="U4B19_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078231.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="U4B19_002562"
     CDS             complement(1490..2938)
                     /locus_tag="U4B19_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2078232.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="U4B19_002563"
     CDS             complement(2938..3654)
                     /locus_tag="U4B19_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2078233.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="U4B19_002564"
     CDS             complement(3635..3985)
                     /locus_tag="U4B19_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEN2078234.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="U4B19_002565"
     CDS             4129..4902
                     /locus_tag="U4B19_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2078235.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="U4B19_002566"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="U4B19_002566"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1123 c          994 g         1948 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DASJTC010000127         5671 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.131, whole genome shotgun sequence.
ACCESSION   DASJTC010000127 DASJTC010000000
VERSION     DASJTC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.131"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            520..1077
                     /locus_tag="U4B19_002567"
                     /pseudo
     CDS             520..1077
                     /locus_tag="U4B19_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            1135..3174
                     /locus_tag="U4B19_002568"
     CDS             1135..3174
                     /locus_tag="U4B19_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEN2078236.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDQYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGEKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKDDDDSATKRASELLQYLNQNYQ"
     gene            complement(3283..4434)
                     /locus_tag="U4B19_002569"
     CDS             complement(3283..4434)
                     /locus_tag="U4B19_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEN2078237.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVFLLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4588..5118)
                     /locus_tag="U4B19_002570"
     CDS             complement(4588..5118)
                     /locus_tag="U4B19_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEN2078238.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5217..5567)
                     /locus_tag="U4B19_002571"
     CDS             complement(5217..5567)
                     /locus_tag="U4B19_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEN2078239.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2069 a         1036 c         1053 g         1513 t
ORIGIN      
        1 aaaaaacata taaaaaaaca cataaaacta aaaaattatc aaagaatcct ggtgcaactg
       61 aaagaatttg caacgggatt ctttcaactt atttgttatt tttttctaaa aaatattaaa
      121 gattgttaaa tttcactatt tcatttcaaa ttccattgcc taatcattag gaaaagtcta
      181 tcatgaactc gtaaatagaa tatagtaata accttgttag gtgaggctcc tatacaaaca
      241 taggctgttg cccagaaacg tcgaaagtcg ccaatgggta aaacagaaac tgttggatta
      301 aggctttttc caatagctaa gaatgttttc tttacgttgt atagtgctaa aactcggacg
      361 atgagatgat gctaggtcag aattcttgtt cgaaaaggtt tctgaccttt tctttatatt
      421 tttgtgactg acttatttga tgagacaaac tcgctcctca cctgacaagt ggattgctgt
      481 gtgaggagga gatgcggtga aaaaagatcg attggtggag gtcgctcgga ttcacttctg
      541 gttgcacaat acaaccagaa aacacaacaa gcaaaactga tctctatcat gagagattca
      601 tatgtcgata ttccaggtta cggaatgaat aagataaatg cagcttactc ttatggggga
      661 gttgatttat taaatcaaac attaaaggaa aatttcaaat ttgaagctcc ctattatgca
      721 agtatcacat ttcaagattt tatcgattgc gttaatgaac tgtttcctgg tggagtcaag
      781 atcgatgcag aaaaatctct agatttagat ggcgtatata taaacaaagg agagcaagtg
      841 atggatggta atactttact ccagtatgct cgatttcggg aagacaaaga aggagacttc
      901 gggaggatca gaaggcagca gcaagtgatc aaagctatct ctcagcagct gaaagacgta
      961 acttcaatat tcaagctacc caaagcggtt gggaaattac ttggtagtat ccaaacgaac
     1021 cttccagaaa gtgtgcttct ggattgtggt atggatttcc tcaaagacat atgttagaat
     1081 aaacaggtat aaatagtgaa ataaaggaat gacaagcaag agaaggagga aaaaatgaaa
     1141 agaagtgaca agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc agtgatctta
     1201 atagcagctg cgggtggcgg ttatttttac taccagcact atcaagaaac ccaagcagta
     1261 gaagctggag aaaagacagt tgagcaattt gtccaagctt tgaacaaggg agattataac
     1321 aaagctgcag aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc tgaaaaagag
     1381 atcttagatc aataccagaa tatatacggt gctgctgatg tcaaaggact tcagatatcg
     1441 aatctaaaag tagataaaaa agacgattct acttacagtt tttcatataa agcaaagatg
     1501 aatacctcat taggtgaatt gaaagatctt tcttataaag gaacattaga cagaaatgat
     1561 ggtcaaacca cgatcaactg gcaacccaac ttggtttttc cagaaatgga aggaaatgac
     1621 aaagtaagcc tgaccacaca agaagcagca agagggaata ttatagatcg aaatggtgaa
     1681 ccattagcaa caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa acttggagat
     1741 ggaggcgaaa aaacagccaa tatcaaagcg attgcttcct ccttcgactt aacagaagat
     1801 gctatcaatc aggcgatttc acaaagctgg gtacaacccg attactttgt cccattgaaa
     1861 atcattgatg gagcaacgcc agaacttcca gctggagcta ccattcaaga agtagacggc
     1921 agatattatc ctttgggtga agcagctgct cagctgattg gttacgtggg agatatcaca
     1981 gcagaagata ttgataagaa tccagaatta agcagtaatg gaaaaatcgg tcgctcaggt
     2041 ttggagatgg cttttgataa ggatcttcgt gggacaacag gtgagaaatt aagcattaca
     2101 gatgcagacg gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa tggcaaagat
     2161 atcaaattga caatcgatgc gaaggcacaa aaaacagctt ttgacagtct aggaggaaaa
     2221 gctggatcaa ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct tgctagctct
     2281 ccaagctatg atccaaacaa aatgacaaac gggatctcac aagaagatta caaagcttat
     2341 gaagaaaatc ctgaacatcc attcatcagc cgatttgcga caggttatgc tcctggatct
     2401 acgtttaaaa taattacagc agcaatcggt ctcgacaacg gcactatcga tccaaatgaa
     2461 gtgttgacga tcaacgggct taaatggcaa aaagacagtt cttggggatc ttttaaaaca
     2521 acgcgtgtca gtagtgatgt atcacaagta gacttaaaaa ctgctttgat ctattccgat
     2581 aatatatatg cggcacaaga aacgttgaaa atgggtgaga aaaaatttcg tacaggctta
     2641 gataaattca tttttggtga agaccttgat ttgccaatta gtatgaatcc agcacaaatt
     2701 tctaatgaag atagctttaa ctcagatatc ttgctagctg atactggata tggacagggc
     2761 gaacttctaa ttactcctat ccagcaagca gcaatgtatt ctgtttttac caacaatggc
     2821 acacttgtct atcctaaatt gattgcagat aaagagacaa aagataagaa gaatgtcatc
     2881 agcgaaacag cagtacaaac gatcgtgcca gatctgagag aagttgtgca agatgtaaat
     2941 ggtacagcac attctctttc tgctttaggg attccgttgg cagcgaaaac tggtacagcg
     3001 gaaatcaaag aaaaacagga tgtaaagggg caagagaaca gtttcttgtt tgctttcaat
     3061 cttgataacc aaggttatat gatggtcagc atgttggaaa ataaagatga tgatgattca
     3121 gcaaccaaac gagcatcgga actattgcaa tacctcaacc aaaattatca ataaaaaatt
     3181 gctttttaag tacagtaaaa ggactgtggc gttatcgtca cagtcctttt ttcaaataaa
     3241 cggttttcac gatgtctctt tcacaacttc tttttgattt actcacactt cataaacttt
     3301 ttttgtaaaa tatttgagaa caagtggaga aataagtgta cttaataaaa tcacgataac
     3361 taaaggtgaa tagtactgtg gttcgatcaa ttgactttgc tgtccaattt gtaaaatgat
     3421 caaagccatt tctccacgcg aaatcatacc agcaccgacc atcaatgaac tatgttgaga
     3481 aaaaccggca atctgagcac caaaataacc tccaagcaat ttggtcaaaa tcgctacaag
     3541 cgtcaatata gaaataaaga gtagttgttc actaaagtca gaaaaatcaa cttctaatcc
     3601 tacactgaca aaaaatactg gtataaatac tgcatatcct aaagcctcta cattgtggta
     3661 tacttcttct ttaacctttg tctgactgac agctatccca gcaaaaaaag caccaatgac
     3721 agagctaagc ccaacaagat ccgctacgta agacattcct aaacaaataa ctagcgatac
     3781 gatgataata gaagaatttg cgtacacttt ttctgcaaat gaaagtagga aaggcgcaat
     3841 ccatttgact aataagaaaa taaaaacaaa ataaattagc tgttcaatca aaacaagcgg
     3901 taaagaagca ttgctagaag cttcccccgt tagaaaagat aggctgaaac ttagaactaa
     3961 aacgactaaa atgtcatcta ccacagaagc acccaaaatt gttgatcctt cttttgtatt
     4021 cactacgttt agttctttta atacttctac tgaaatactg acagaagttg ccgcaagaat
     4081 aatcccaaag aaaatcgctt catttcccga aacttggaat gctttgcctg tcaaccagcc
     4141 aaaaacaacc gggaagatga tcccaagcaa agcaacgaac ataccaggtc ggaaatattt
     4201 tttcaaaaga gataaatcac tttccagacc agctaaaaac attaataaga acacaccaat
     4261 ctccgaaaag tcatggacta gaatatcagg tttgacccaa ttcaatccag cttggcctag
     4321 caaaacacca acaagtaatt gtccaatcac tgctgggata cctaatctcc gcgagatatg
     4381 tgagccaatc gttgttgaaa tcaatatcag gcataaaata ccaataaatt ccattctcat
     4441 cattcctttg tataaaatat agcccgaact tagcataaca tacattattg aaaaaatagg
     4501 aaaatatact cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt tggtttttat
     4561 acggtctatt ttaataacta aaaatgatta acgtatagtc atttttctta ttctgatgaa
     4621 attgataatc tcctggatgc ccatgaaaat caaaataaag ccgaatattt ggaatagaag
     4681 catcaaactt ttgaatggat tgaacaataa gacgaatccg ccaatcaaca acagaatact
     4741 actataaaca ctccaacttt tagcttttgt attcatgctg aagaacaact ggaacaagct
     4801 gttgatgata acagctaatc caagaagaat cggcaggata gaaacgattg caggtgcaaa
     4861 agcatagacg atcagagcta agatcaggaa aaaaagacca ctgaacagac cgatactcca
     4921 agaacctgtt ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc caagcaaaac
     4981 aagataggcg gtgatcagat aaccgataaa atgaaaaaca gcagaagggt ttagcacaat
     5041 cgcaatccca gaaataatat aaacagcagc tcttaataag gcgtattttt gaatattttg
     5101 tttaatcgtc tcaaacatat aagaaaacct cctttgactt attttaacgt gaactagtca
     5161 ttttaccaaa ttaaatgtta caaaatgagc gaaggagaag aatatctctg gaaaaatcaa
     5221 cttctgagga tcttttccat ttttttgcct tttgctagct catcgatcat tttatctaaa
     5281 taacgaattt ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc acaaacaaca
     5341 cctgtaatca aggaacgtga aggattgggg acaggggctt ccgcaaaaaa ttgttcgaaa
     5401 gtcacttgtt tttgtagttg atattctagt tctgtttggg aataacccgt cagccaacaa
     5461 atgatttcat ctacttcctc ttttatacga tcttttctct ccgcttttgc tacatacata
     5521 ggataaacgc ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat cagtcacctt
     5581 tctttttttc aaattattta ctacttatag tatataagaa aaagagaaga aaaagcgatt
     5641 attcaaaaaa acaaaaagga ttgtgacaaa a
//
LOCUS       DASJTC010000128         5469 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.132, whole genome shotgun sequence.
ACCESSION   DASJTC010000128 DASJTC010000000
VERSION     DASJTC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.132"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(52..1557)
                     /locus_tag="U4B19_002572"
     CDS             complement(52..1557)
                     /locus_tag="U4B19_002572"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEN2078240.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1649..2239)
                     /locus_tag="U4B19_002573"
     CDS             complement(1649..2239)
                     /locus_tag="U4B19_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2078241.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2418..4064
                     /locus_tag="U4B19_002574"
     CDS             2418..4064
                     /locus_tag="U4B19_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2078242.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4113..4832
                     /locus_tag="U4B19_002575"
     CDS             4113..4832
                     /locus_tag="U4B19_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEN2078243.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5311..>5469
                     /locus_tag="U4B19_002576"
     CDS             5311..>5469
                     /locus_tag="U4B19_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEN2078244.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISA"
BASE COUNT         1651 a         1000 c         1101 g         1717 t
ORIGIN      
        1 cacagtccct ttttttctta gaaatgggaa accactagta tataaatacc attattcttc
       61 aatcaaccgc ttcacgattg cataatcccc ttcataaggt tcatctacac cattgatttt
      121 ttgataggga tccacgcctt ttcccgcaat aacgactgaa tcttgcggag cgctttctgc
      181 taaagcgcgt cgaatcgctt caggacgatc catttcataa tgaacgatca aatctggatt
      241 agtaattgct tcattgattt cttccgcgat ttttttcgga tcttcaaatg ctgggtcatc
      301 tgccgtcaaa aaggcgatgt cagctgtttc tgatagcact tttccaaaat catgtcgacg
      361 ggagattgct ttgtttcctg ggctgcctaa tacaacaatt acacggccat ctggatgttc
      421 atttttggca aattccagca atgttttcaa gcttagataa ttatgtgcat agtccacata
      481 gacatgcgct ccattggatt ggatcaattg atccattcgg cctggtacac gagcttcttt
      541 taatccgtct tgtgcatctt gactggaggc acctgttagt gctgcggcaa tgatcgcact
      601 ggctgcattt tcttggttga atcctccagc cagcaaaata tcataagcag tatcgcagat
      661 agctaagcga tcttcatgag aagataaagt aaaaccgtga gtacctttat ctgaacggat
      721 cacttgataa ttgctttctt ccgaacgacc ataagtgatc gttggaatct ggaatagatc
      781 ggctgtttcc ctaagtaaat caaaatagtc actttcatga tttaaaacaa ctgtttttga
      841 attcaagatc aattgtcgct tgcaatagaa atagtcatca aatgtcggat gttcgatcgg
      901 actgatatgg tcaggcgaga tattcaagaa gattccgaca tctaaagtca agccgtatac
      961 gcgttgtgtt ttgtaagctt gtgaagaaac ttccatcact agatgagtca taccattttc
     1021 aactgcttct gacatcatac gatataaatc taatgactca ggcgttgtca aatgggattt
     1081 gaaatatgtt ttgccatcta acgttgtatt catagtagaa agcaatgctg tttttttatt
     1141 tgttgtgtga tccaagattg cttttgtaaa gtacgcagca gttgttttac cttttgttcc
     1201 agtgaatccg atcactttca atttattttg cggatagtcg taaaaagcca tagcaagaag
     1261 cgccattgct ttacggatat ccgttacgat gattcctact gctgaatttt cgtatggttg
     1321 ttctgataca taatattgaa taccctgttc gattgctgaa tccaaatatt cttctttgaa
     1381 attcattcct ttacagaaaa acaaagtaga agcatcagct tttcttgaat cataacttaa
     1441 ctgaagaaat tcagcttttt ctggtacatc aagatgccag cctcttgttg aaacaaattc
     1501 ttttaataga ttttctttta atagtaaatg acggatgtct tctaaagaga agctcattga
     1561 ttgatccctc gctttattca atttcttggt ttattatagc acactctttt taatatatcg
     1621 tttgtttccg ctatgacaaa atccagcttt attcaaattt tcttgacaca ggggacaagc
     1681 ggatgagtat tttcataaac aatgaactga ctgtaataga taggagaacg aatagtgtct
     1741 ccaacaatcc aaaaacataa taattcaaaa agacgataag actattggta agtaaaagtc
     1801 cttgcggcat tggaagccga aaaaatttgt ggaaaataat cggcggtatc gttataccgc
     1861 catttgaggc cccgactgtg tacaatgtgt aaaccccaag actgaacaaa agacttcctg
     1921 aaatcagtga aaagaaatgt gaggagatca tttccacttt tggaacaatc tctaaaacaa
     1981 caggaaaaag cagacttccc gctacagtct taaaaaatac tgattttcct aataggacag
     2041 cggctagaag cagcatcata agattgatgc tccaaacagt aactgagcgt tggatagaaa
     2101 aggccgtttc aactaaatgt ccgatggcgc ccactccggc ggaagctata tcgtgaggga
     2161 gcaagaacca attcaatcca actgtgataa agaaaagacc taaaaatata acgggtagtt
     2221 tttttatttc ttgtgtcatt gatatctcct ataaatgtta gccataaaag gctgtatatt
     2281 tctttacttt accaaaaaaa cgaaaccgaa ttcaaaaaaa caattgttct tataaaagtt
     2341 atataaaatc gattgccagt ttctgtttta ccaattaaaa tgatagtaat gttttgtaga
     2401 aagttggaat ctgaccgatg gataatcaaa gaaaaccagc attagatcaa ttaacaccgc
     2461 aagaaaaaat ggcacgaggc tctgcttgga tgaccgccag taatatcatt tcaagattgc
     2521 taggcgctgt ctatatcatt ccctggtatg cttggatggg cgaaaatgct aaggctgcca
     2581 atggattatt caatatggga tacaatatat atgcattgtt tctactgatc tctacagcag
     2641 gaatacctgc tgcgattgca aaacaaactg cacgctataa ttcattgaat gaatatggca
     2701 cttcacgacg attatttatc cgtgcattac agatgatggg cgggctaggc ctgttgtttg
     2761 ctttgttcat gtacatcgcc tctccttggc tagctcatgc ttctggtggc ggagaagaac
     2821 tgattccaac gatgcgttct ttgagtatcg ctgtattggt ttttccttgt atgagtgtga
     2881 tacgagggta ttttcaaggc aatcaagaaa tgatgcctta tgcgctgtcg cagattgtag
     2941 aacaggtggc acgtattttt tatatgctat tggctacatt catcatcatg aaagtcatgg
     3001 atggtgacta tgtgacggcg gtcacgcaat caactttcgc ggcatttatc ggtatgctag
     3061 caagtattct tgtccttttg tattttttga agaaacatca agcgtataca tcggcgttta
     3121 ttcactacag cgaaaacaaa ataaatattg caacgaagga attgttgtta gataccgtta
     3181 aagaagcgat tccgtttatt attgtgggtt caggggtaac gatcttcaaa ttagtcgatc
     3241 agttcacttt tatgaagatc atgtctgcat ccaccgagta ttccaatgca cagttactgg
     3301 atcttttttc gatttttagt gccaatccag ataagttgac gatggtagtt attgcattgg
     3361 caacatcgat cgcgtctact gggttaccgt tgattacaga agctgttacg ttgaaagatc
     3421 gagtaggtct ggcaaaattg acaagcggaa atttacagtt gttttcattt tttatgtttc
     3481 cggcgacatt tggtgttatg ttacttgcct atccgctaaa tacattattt tatacaccgg
     3541 attctctagg aagtaatgta ctgatccaag caagttttgt aggactcttc ctaggactat
     3601 atatgctagt ttccaacatg ctgcaaggaa tgtttgaaaa caaggcggca attaattatt
     3661 tggttattgg gtttctagtg aaactccttc ttcaataccc agccattcgt ttatttgaag
     3721 tatacggccc tttattggcg acaatgatcg gatttgctgt gtcttgtacc ttgatcttaa
     3781 agagaatcca tgaagtagcc cgctttaaac caagttttgt ctggcgcagg acattgctga
     3841 tctttattct taccttgatt atgcttttgg cttcgtggat cacaaagatg atttttggtg
     3901 cttttttaaa cgaagatagc aaattccagt ctctattgtt gattcttgta gtagcaggta
     3961 taggggcttt agtgtatggt tatttagctt tgaaaatccg cttggcagaa agactactag
     4021 gaccaggaat agcgcgttta cgacataaat taaaaataaa ataagtgaat cagaagagct
     4081 agattgatgc tcttttgaaa tgggggaaac gcatgcgttt agataagttt ttagcagata
     4141 tgggacttgg cagccgcaaa gaagtcaaag gattgatcaa aaaaggaacg atcgaagtca
     4201 atggacaagt ggtgaaaagc gataaagcgc aagtaaatga attcgaagat gcggttactt
     4261 atttaggaga aatcattacg tatcaaaaag atttttacta cattttgcat aaacctgcag
     4321 gagtcatttc tgctactcag gataatcatg atcaaaccgt aatcgattta ttggaagacc
     4381 aggattatcg ggaagatctt tttccagtcg gccgattaga taaagacaca gagggcctct
     4441 tgattttaac gaatgacggg gtgtttgccc atcagttact atcacctaaa aaacatgtgg
     4501 agaaagaata tctagctgag gttagcggta taatgacaga agaagatatc cgtttatttg
     4561 cagaaggact gattattgat ggcgatgagc agacattgcc ggcaaaactc caaattgatc
     4621 aagtagatca agagacagaa acttcaatta tccgtctgat tttgcatgaa ggaaagtttc
     4681 accaagttaa gcgaatggtc aaagcagtag gaaaagaagt cttatattta aaaagaatcc
     4741 gtatgggaaa attttggctg cctaaagatt tgaataaagg gcaatatcga cccatgacca
     4801 aagaagagct agaacaagtg agagaaaaat aattgaatgg atagctaaaa gaataagcag
     4861 agtagttgta gcgccatctg aaaagcagac ttgtccgtct gactttcgga tggctttttt
     4921 gtgaaatttc ggcttatttc cgtagaagct acttctggaa caccgtttgt tcggaataag
     4981 ggactgtgac aagacttttg ttacagtccc cattgttcct tgtgactcag agttagatag
     5041 taaggacacg atctccctaa tactcaggcc aaaacgctga attcacaacc tctgataaac
     5101 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
     5161 tttcccgttt ttttctctaa tactcaggcc aaaacgctga tttcacaacc tctcctatac
     5221 tttgtttcat tttccctata ctttgtttat ttattctcat tattgaaagc gctataatta
     5281 cgttgcgaat gaatcgaaag gggtttttag atgaaaaaac gaaaggggtt caatcatttt
     5341 tttatgaatc tatggcgtta taaagcactt gtcctgatgg ctgtaccggg tatgctttgg
     5401 atgatttttt tcttttacat acctgtaatg gcgaatgtcg tagcattcaa aaattttcat
     5461 atttcagca
//
LOCUS       DASJTC010000129         5326 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.133, whole genome shotgun sequence.
ACCESSION   DASJTC010000129 DASJTC010000000
VERSION     DASJTC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.133"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            105..1037
                     /locus_tag="U4B19_002577"
     CDS             105..1037
                     /locus_tag="U4B19_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078245.1"
                     /translation="MIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEEDK
                     KSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEVVY
                     KNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYLLS
                     EKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFVVS
                     ESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQNGL
                     ETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            1258..3021
                     /locus_tag="U4B19_002578"
     CDS             1258..3021
                     /locus_tag="U4B19_002578"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEN2078246.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            3105..3563
                     /locus_tag="U4B19_002579"
     CDS             3105..3563
                     /locus_tag="U4B19_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEN2078247.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            3672..4355
                     /locus_tag="U4B19_002580"
     CDS             3672..4355
                     /locus_tag="U4B19_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN2078248.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            4502..5116
                     /locus_tag="U4B19_002581"
     CDS             4502..5116
                     /locus_tag="U4B19_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEN2078249.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            5132..>5326
                     /locus_tag="U4B19_002582"
     CDS             5132..>5326
                     /locus_tag="U4B19_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078250.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNY"
BASE COUNT         1886 a          843 c         1102 g         1495 t
ORIGIN      
        1 tcccagaatt tgactcgcgg aacatcttgc tagctttagt acgttatgtc aattgggaag
       61 gctatgaaaa taaaaatccg tatgtagaag aagcttgtaa cgagttgatt gattatgtag
      121 agactcattt ttttaaaaga agatattcgg aagatgaaaa gtcctttatc atacgaggaa
      181 tagagatcgc aatcggaaga aaggcatttc cccttggact gaaagaagaa gataagaaaa
      241 gcgcagaaac gaagccgtta tttcaattag tctgtcaagg attggaagag caaaaaggaa
      301 ttctagactt acaagaagaa gatgtctatt atattttttc tttgttcaac agtagaaatt
      361 ataccaatga gaacatggag ctgttaagaa aagatttcga agttgtttat aaaaattttg
      421 ttctcaatga cctatcactg aatgaattga ttttacagat tattagtatc gctgaaatcg
      481 acgatgcaga aaattttatt ttcaagcaat cctttttaca atttgtccgt acgttgtggg
      541 cagatggaca agtattttta ccggaaagga tctatcttct atcagaaaaa gaacaacttc
      601 tgtacggaaa aattgtagaa atattggaag agtggaaaaa gaaaaatcaa atccaactaa
      661 gatggaatga aaatttcatt cgaaaattcg tcaaaagtct ttctttgatc aacagtgaac
      721 aagcggggag acaggaaata gaaatttttg ttgtttcaga aagttcagtc aaacaatttt
      781 tttatcgaac ccaatttcat ttgtatgcgg aagaaggcat cgctgaaata aatcctattt
      841 tatttcgttc attagaagaa ttgcctgatg aatgtctatg tgcgaaaaaa agagtggttt
      901 tgtgcgatgc tgctagctat caaaatggat tagaaactga aaagacaaag atctatccaa
      961 tctcattaaa aggtgcaaac atccatttgt atcatattgc tcaggaactt cacctattaa
     1021 aaaaacatat ttcttgatga gaccttaatt aggagacctt ctacaaagct ttttaattgt
     1081 ctgtagaagg tttttatttg agtcctgatt cgtgcgaaga tctacttata taacaagctt
     1141 tctctgaaat ttttttaaaa tgctattgac ttagagtcaa ctctaagtag tagaatagat
     1201 aaagtgtaac aaatctattt tcattaaaag aagatagtac ggaggagaga atgtattatg
     1261 agtacagtat tacatgtatt ggatgcaatc aatagccccg cagatgtcaa aaaattatct
     1321 gttgaggaaa tgaaacagtt ggcagaagag atgcgccaaa aaatcttgca aaaggacagc
     1381 gatgtaggtg ggcacgttgg tccgaatcta ggtgtagtag aattaacgat tgcgtttcat
     1441 tatgtatttg attcaccaaa ggataaaatc gtttgggatg tttcccatca gtcttatggt
     1501 cataagcttt taactggtcg aaaaaatggt tttgaaaaag gacattaccg tgaagtgagc
     1561 ggatattcca accaacatga aagtaaacat gactatttta cagtggggca cacatctaca
     1621 tctatcagct tagcaacagg tttagctaaa gcgagagatc tgaagaaaga atcaggaaat
     1681 gtggtagcat ttatcggtga cggctcccta agtggtggct tagcttttga aggattgaac
     1741 aatggtggtg ttttacaatc caatctaatc gtattagtaa acgataatga gatgtctatt
     1801 gatgagaatc atggcagttt atataaaaac ttagctgagc ttcgtcaaac caacggtaaa
     1861 agcagcaata acctatttaa agcaatggga tttgattatc gttacgtgga taatggaaac
     1921 gatctggaaa cggtgatccg tttatttgaa gaagtaaaag atattgatca tccgattgta
     1981 cttcacgtcc atacggaaaa aggtcgcggt tatcagccgg caatcgataa taaaatgaaa
     2041 tatcattggc atgtaccatt tgatctgtcc accggggagg caaagcaagt aacttctgga
     2101 gaaacattta atatgatcat catggattat ttggataaga aaatgcaaac aagtacagct
     2161 cctattgccg cagtcaatgc agcgatacca ggcgcctttg gactgaaaac attccaagaa
     2221 aaacatccag atcattattt tgacgtgggg atcgctgaac agcactctat cactttcgct
     2281 tctgctttgg cttctcaggg agtacgtcca gtcgtcttcc aatcaagtac tttcttgcaa
     2341 cgggcatatg atcaattatc acatgattta gcgatcaacg agaatccagt gattatttta
     2401 gtcggtggcg gaacgatctc agctagtgac cgaacacacc aaggaacatt tgacattcct
     2461 tacttatcaa gtatcccgaa catcaaatat cttgctccag caacaaaaga ggatatgatc
     2521 tctatgctag attgggcgtt cgaacaaaca gcgcagcctg tagcaatccg aattcctaca
     2581 catggtgtag aacatggtac agctatcgca aatgattata gtattccagc ctatcaaaca
     2641 gtaaataatg gaggaaaagt agcaatttta gctttaggcg gttttcttga attaggaaaa
     2701 gaaacagtaa aagcttttag aaaaaaagga atcgaagcaa cgttgatcaa tccaggaacg
     2761 atcacagaaa atgacgtaga aacattagat aaattaaaag aaacacacga attagtcatc
     2821 acattagaag atggtagttt gtcaggagga tttggcgaaa atatcagtcg ttattatggt
     2881 gattcttcaa tgaacgtatt aaattttggt gcagagaaag aattcacgga tagcgttcct
     2941 gtagggcagc tttatgaacg ttatcattta acaccaaaac aaatcgttga agatagtctg
     3001 cgtgtcttga atattcaata attcattatg ctcaccatcc agataattgt ttatactagt
     3061 atctggatgg ttttttcatt ataaaaagca aaggggtgga tgttatgtat cagcaagtag
     3121 aagaatatgt caaaaagttc caagaaaaat atcctagtta tcaatcagaa aaattaggtt
     3181 tcttttcatt tggggataca gaaaaaatgg cagacgagtt gagcgaacta gttgtaaaaa
     3241 agataaagac tggtacatca agtggttacg atctgtataa acaagagaat gagcctttac
     3301 caaaagttgg tcaattagac gtggtcctga atggaaaaaa tgaaccagtt tgtatcatca
     3361 aaaatataaa tgtgaccatc ttgcctttta gtgaagtcac gaaagagcat gcttggaaag
     3421 aaggagaagg ggatcggaca ttagcttatt ggcggaaagt ccatgctgag ttttttaaac
     3481 atgcttataa agaagcgttg aacatagaat ttacagaaga gaagctggtc gtatacgaag
     3541 aatttgaagt aattttcttt tagtaagaaa ggtctgctga aagaaatagc tcattattgt
     3601 ctcctgaata gaaaagggtt acatttttat atgaagctgt atttttatat aacattactg
     3661 gaggagatat tgtggataca ctggaacaaa tcaaaggcgg agtcgtcgtt tcttgtcaag
     3721 ctttggaaaa cgagccgcta cacagttctt ttattatggg gagaatggct ttggctgcaa
     3781 aagaaggcgg agcagttggt attcgagcaa atacgacaga agatatcaat gaaatcaagc
     3841 gaatcgttga tttaccggta atagggatta tcaaaagaga ttacgatggg tcagaagtgt
     3901 ttattactgc gacagaaaaa gaagtggaag aacttttgga gacgcaagca gaaatcattg
     3961 cactggatgc aacttctcgt aaacgtccga cagaaaaaac aactgctgaa cttgtccaaa
     4021 tgattcacga acacggtcgg ttagctatgg cagatatttc gacttttgag gaagcgcttc
     4081 aagcgcaaga agatggtttt gatcttttgt ctacgacttt agcaggctat acagaatatt
     4141 cacgaaagac gcagacacct gattttgatt tattgaatca aatcatagaa caagtcgata
     4201 tcccagtaat tatggaagga catactgact ctcctgaaca agtagagaaa gcatatgaat
     4261 taggtgcttt ttcagtcgta gttggatcta ttatcacccg tccgcaattg atcactaaaa
     4321 gatatgtcga agccgcaagt aaagcaaata actaaaaaac acgcacggca cagattcttg
     4381 acaaaaaaga aaaattgata gatagtttaa agcagattct aagaagatga aaaaagcatt
     4441 acttttcatt ttgtcttcaa gatctgcttt ttaacgaatt ttatagaaag aaggcgaaac
     4501 catgtttgaa cggagtcctt cagaattgac agaacgtgaa aattacaagt tgctgattgg
     4561 ttcagtcatc cctagaccag tagcagtagt aacgactaaa tcagcaaaag atatagtgaa
     4621 tatcgcaccc ttcagttact tcaatatcgt cagttcgaat cgacccatct tatccttggc
     4681 gatccagaga aaacaagatg cagtaaaaga cacagcaaag aatattcttg agacaagaga
     4741 agctgttatc catatccttg atgaggacaa cgtagaagaa gtaaataaaa cagcagccaa
     4801 tctgcctaat gaggaaagcg aactgtacct gactgacttg actttaactg attcagtaat
     4861 catttcagta ccagcattga cagaagcaaa agttcgtttt gaaacatcct tatatgaaca
     4921 tgtcgagata cggaataaaa ataaagtaac agctgattta tttttgctag aagtacagaa
     4981 atatcattta aatgaaacag tctatgatga taagacagga aggatcgatc caagaaaatt
     5041 gaatgctgta agccgtttgg caggaaatga ttatgcagca ataggagaaa ttttcacgat
     5101 tgcacgtccc gaataaaaag gagcactagt tatgttaaaa ataagctata aaccatcgac
     5161 agattcaaaa gaaatgaaaa aagaatatga aacagtgaat gattttctgc aagggcagta
     5221 cttagaagtt ccgccattgc aagaccattt tgtcgttact actgttactt tagatggtaa
     5281 ggaaatagaa atgccagatc aaacgattag cggattgttt aattac
//
LOCUS       DASJTC010000130         5312 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.134, whole genome shotgun sequence.
ACCESSION   DASJTC010000130 DASJTC010000000
VERSION     DASJTC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.134"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="U4B19_002583"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="U4B19_002583"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEN2078251.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="U4B19_002584"
     CDS             complement(1297..2133)
                     /locus_tag="U4B19_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2078252.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="U4B19_002585"
     CDS             complement(2317..3534)
                     /locus_tag="U4B19_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEN2078253.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3862)
                     /locus_tag="U4B19_002586"
     CDS             complement(3683..3862)
                     /locus_tag="U4B19_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078254.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3917..4000
                     /locus_tag="U4B19_002587"
     tRNA            3917..4000
                     /locus_tag="U4B19_002587"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4818)
                     /locus_tag="U4B19_002588"
     CDS             complement(4240..4818)
                     /locus_tag="U4B19_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078255.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4824..5207)
                     /locus_tag="U4B19_002589"
                     /pseudo
     CDS             complement(4824..5207)
                     /locus_tag="U4B19_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1738 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa gcttattatt
     4981 gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa actaactata
     5041 cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa tatcaataaa
     5101 ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag ttgaaaaaac
     5161 ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt ggttgctctc
     5221 caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca aaccaataat
     5281 aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DASJTC010000131         5161 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.135, whole genome shotgun sequence.
ACCESSION   DASJTC010000131 DASJTC010000000
VERSION     DASJTC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.135"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            230..1450
                     /locus_tag="U4B19_002590"
     CDS             230..1450
                     /locus_tag="U4B19_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEN2078256.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1447..4167
                     /locus_tag="U4B19_002591"
     CDS             1447..4167
                     /locus_tag="U4B19_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2078257.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4151..5095
                     /locus_tag="U4B19_002592"
     CDS             4151..5095
                     /locus_tag="U4B19_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEN2078258.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         1916 a          844 c         1026 g         1375 t
ORIGIN      
        1 ataaattcca gtacaaatag tacttcttca gtgtcaaacg aagcgaatat aacatagata
       61 tttaggattg tgacaaaaat catgtcacaa tctttttttc gaataaactt attttttgaa
      121 gtcggccatt tatcgtaatt tctttgcata atacgaacaa attttcggtt attgatgaaa
      181 aaaaacattg aaaatggtat gataaaagaa aaaaaggagg aaatttttaa tggtgaaatt
      241 tatccatgcg gcagatttac atatggatcg gtcatttgaa ggactgacaa ctcttgataa
      301 aactgtacag gaaaaactat tgaagacgaa tctgaccgtt ttatcaaata tcattgaaca
      361 ggcaataata aataacgttg attttgtttt acttgcagga gataattttc atcaaaatcg
      421 tccttcatta aaaatacaga aacatttttc agaacagatg aaacggttag aaaagaacca
      481 tatacctgta tttataatat tcggaaatca tgatttctat caaaaagagc ggtattggtt
      541 ctcctttcct aaaaatgtcc atttatttac atccgaaacg gtagagacaa aaaaaataac
      601 gataaaaagt ggagaaactg tctctctcag tggatttagt tatcgtcaac cgtggattca
      661 aaaagacaaa gtgatggaat ttccttctag agaactcaca gattaccata ttgggttgta
      721 ccacggggaa cctggcgtct ctcaaaaagg aaactatgct cctttccaac cctcaaagat
      781 gcaagaaaag ggatacgatt attgggcttt agggcatatc catgtgccaa cggtcttaaa
      841 tgaaaagcaa acaattgttt atccaggtgc gcctcaaggt catactaaaa aggaacaagc
      901 ttctacatct attctactag ttgaattaag taaaggaagt tgccaaatac atccgattaa
      961 agtagcagag gtctattgga agacaaagga aatatcattg aggaaagcaa gaacgacaaa
     1021 agaggtcatt gttcacattc gtcagcagct ttcaaaagta gaggatggat ttagtttaat
     1081 tgagataaaa ttaaaagatt atgaccatct caatacagat gttttggaac ggatacaatc
     1141 aggagagcta ttagactatt tgttagagga attctcagat aggataaatg atttctttat
     1201 ttggcgtatt tctctcatag agaatacttt ttatacgaag accccacttg ctgcttctgc
     1261 taaattgatg gaacagttat ttcaatacta tcagacacct caagatttcc aacaaatttt
     1321 aaatgaagta tatagtcatc aagaagcggc tagaatattg agcgaactgc cggaatatca
     1381 agaagaaaca cttgaaaaag ccaagcagct gcttaatcaa gattttttat ttgaggagga
     1441 tcaggaatga agattttaag gatcgagatt gctgcttttg gaaaatggcg tcaaaaaagt
     1501 tttgactttt attcaggcaa tcagctgatc tatgggggaa acgaagcagg aaaatcaacg
     1561 atttatcaat ttattcaagc aatattattt ggtttccctt caaaaggccg taaaaaaaag
     1621 gattatacac caaaagacgg ctcagcctat ggcggcaaaa tatggcttaa acatcctgta
     1681 tatggtgaat ttgctgtcga gcgctacaag cagcaaaatc gcggaaaatc gaaggtgtgg
     1741 ctgggggatc aagtgggaag tgatgagtta ctagaaaaac tgatcgcacc tttgacgaaa
     1801 gagttattcc agcaggtatt tactttccag caagagcagt tgatggaact agatcatttg
     1861 agagaaaaag agctacatga tgcgctgatt tctttaggca tcacgggcag ttctcaagta
     1921 ttccaaaagc ggcaagaata ttatcagaaa gcacaacaat tattcaagaa aaaaggccaa
     1981 aaattactaa tcaatcaaaa attaaatgaa tggaaagagc tgcaagaaaa aattcatcaa
     2041 aaacaagaac aggaagctta ttttggcaag ttgatccaac aagaacagga atacgagtcg
     2101 caacagcagc agttattggc acaattaaaa gaggcgaaca aacaattttc attagccaga
     2161 cagcaagaac ttaatttttc tttatatgaa gaatggcaat ctctcaaaaa aaacagcact
     2221 acaggtcttc ctgatgagca acttctttta gatctggagt ctttctccaa acggtatcag
     2281 caaacaaatg agcgtttgga agaattagaa gcaacactgg aaaagcatag cgggatggat
     2341 cagcagtcag ccagatatta tttttattta gagcaggagc aggaactaaa agatctccag
     2401 caacttcaat ttacagcgga acaattaaac gaagaatggc aaagattaca aattgagact
     2461 agagaaacaa attcagaatt gaagattttg gaacaaaggt ggcattggca gaaaaatccg
     2521 ttaccggaag aatttttata tgaagaagaa tggaagcatc ttctggctga agaaagaagg
     2581 attgctgatc tagaaagcca gatttctgtg cgtctccaaa tagctaaaga acagcaagag
     2641 atgattgaag cagaagtgaa tgacttggaa gagacctatc cagcattatt acaaaatcag
     2701 tcagagatag caccagtctc ttcatctaaa ccgtatatgc tgatggctgg cggagcaata
     2761 atcattgcag gtatatttgt accaatgccc ttgaaagtga tccttttact aattggtgca
     2821 ggcatgattg gctatttctt ctacaaaagg caaccaaaaa ttaaaactaa aaatgatttc
     2881 tctgagataa aaggaatgtg gcaagaaaaa ctaggacaac ttgatctagc cttagctcaa
     2941 ttagctgaag cagaagaaaa acaaaagaga actggagaag aaaaacagat tcttcagcaa
     3001 gaagtatatc gttttgcgga tcatcggcac ttaggaaaga tggatacgct caaactcatg
     3061 aaagaatatg gacaggaagt catagattat cagaaattat atggcgtatt tgagcggaaa
     3121 cataaaagag aacaggaaat acaagaaaaa ctacgacaaa tcgaccaaaa atttgagttt
     3181 gttaaagaat ggttaccgat acaagataag acaatttcag aaaaaatggt attgctttcc
     3241 cgttttatac aagagatgga acagatgaaa tttgccagaa gctatcagca aaatacttta
     3301 ttgaagcaag aaatcaatca attgaaaaag aaacaaaaag tatggataca agaatacagc
     3361 gaacaattga ctcaagcagg cttatcttat ccttcggaag tacctgtctt tttacaaaaa
     3421 gccaaacaag ccgaacagca gatgaaaaga tacaatgaat tatcggaaat gcttactcca
     3481 ctatttcctg aagagctgac aaaagagaaa ttatctgtaa gactgcgcca actgaaagaa
     3541 aagcaatccc aactccagca agaagaaaaa gaagcaagtg aaaatcggca acgactccaa
     3601 cttcaagtag aagagttgca aagagacggc acattggacg aactttatca acaagctagc
     3661 cggcaaaaag caaaattaca ggaattactg gaggaatggg gagcacttga aatcgccggg
     3721 acattcttag gtgatctttc aacagagatg agtgaaagac aattacccca gttgttaaga
     3781 gcagcaggtg attatttagg tattttaaca aaaggaaatt atcagaagat attactgaaa
     3841 aatgatacac tgactttaac agacggatat acagaatttc ccatctatga gttatctaca
     3901 ggtacgaaag atcagctgat tatggcgatg agatttgctt acctctcatt agaagctaac
     3961 cgttcgatat gcccgatcat cattgatgat ggctggcttc attatgatca tcagcgaaag
     4021 tatcaattag ctgaactttt agctaaattt ggagaaaagt atcaaataat ttgtttttct
     4081 tctgataaag aaatggtaag ctattaccat gagatgaacc aaatcgtaaa acaattagaa
     4141 ggagcaaata atgaaaaaaa ttcgtgattt agtaatcgat gagcagtttg aacaatttgt
     4201 cctgatcaaa agtgcagatg taagaatcgc taaaaacgga aaaaaattta ttgcttttac
     4261 tttccaagat acttcaggta ctatcgatgg taaatactgg gatgcttcgg aggaggaaat
     4321 caataaattt acagccggca cagtcgtttt tctaaacggt aaaagagagg tttatcaagg
     4381 aaaccctcaa gtaaagattc ttcatatgcg cttaacaagg gtagatgagc caggagagcc
     4441 tagtttatac atggaaagag cccctttgaa gaaagaagaa atggaagaag aaatcaatca
     4501 attgttgttt gagatcacca atgcgcattg gaatcgtatt gttcgctatc tacttaataa
     4561 atatcaaaaa caattttttg agtttccggc agcaaaaaga aaccatcatg catttgccag
     4621 tggattagct tatcatactt tgacaatgct tcacttaggg aaatcaatcg ccgatgaata
     4681 tcctgagctg aatcgagcat tgttgtatgc tggtatcatt ttgcatgatt taggaaaggt
     4741 gattgaactt tccggtccta tgtcgacaga atatacgctt gcaggaaatt tgatcggtca
     4801 tctggtattg attgacgaag aaataactaa agcctgtctt gcgttgaaaa tcagcgaagc
     4861 tgacgaagat gttctcgtat tacgtcatat ggttttagca caccatggct tattagaata
     4921 tggctcacct gtacgtccaa gaataatgga agcagaaata ttacatcaaa tcgacaatat
     4981 cgatgcttcg atgcagatga tgttgacttc gattcgtcaa acagaaccag gaaaatacac
     5041 cgatcggatt ttcggtatgg acaaccgaag tttctatgta ccaaaagatt tatagcaaaa
     5101 atttattttt ttcattcaaa acaaaaaaag cgagacataa gtcttcgctt tttttgtttt
     5161 c
//
LOCUS       DASJTC010000132         4947 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.136, whole genome shotgun sequence.
ACCESSION   DASJTC010000132 DASJTC010000000
VERSION     DASJTC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.136"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="U4B19_002593"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="U4B19_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEN2078259.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="U4B19_002594"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="U4B19_002594"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEN2078260.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="U4B19_002595"
     CDS             2591..3376
                     /locus_tag="U4B19_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2078261.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="U4B19_002596"
     CDS             3389..4240
                     /locus_tag="U4B19_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEN2078262.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="U4B19_002597"
     CDS             complement(4364..4816)
                     /locus_tag="U4B19_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2078263.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1672 a          810 c         1034 g         1431 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctcta
//
LOCUS       DASJTC010000133         4933 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.137, whole genome shotgun sequence.
ACCESSION   DASJTC010000133 DASJTC010000000
VERSION     DASJTC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.137"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            85..972
                     /locus_tag="U4B19_002598"
     CDS             85..972
                     /locus_tag="U4B19_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEN2078264.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="U4B19_002599"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="U4B19_002599"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEN2078265.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="U4B19_002600"
     CDS             2274..2720
                     /locus_tag="U4B19_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2078266.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="U4B19_002601"
     CDS             2993..4345
                     /locus_tag="U4B19_002601"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEN2078267.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="U4B19_002602"
     CDS             4516..4803
                     /locus_tag="U4B19_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078268.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DASJTC010000134         4863 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.138, whole genome shotgun sequence.
ACCESSION   DASJTC010000134 DASJTC010000000
VERSION     DASJTC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.138"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(89..1699)
                     /locus_tag="U4B19_002603"
     CDS             complement(89..1699)
                     /locus_tag="U4B19_002603"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEN2078269.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(2892..4232)
                     /gene="glnA"
                     /locus_tag="U4B19_002604"
     CDS             complement(2892..4232)
                     /gene="glnA"
                     /locus_tag="U4B19_002604"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEN2078270.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(4260..4652)
                     /locus_tag="U4B19_002605"
     CDS             complement(4260..4652)
                     /locus_tag="U4B19_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEN2078271.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1434 a         1046 c          797 g         1586 t
ORIGIN      
        1 taataacaaa tcagaaaaag agaaaagtga caagaaaatt gcttatcccc ttctcttgct
       61 acttttctct ttctatcaag ttcaaaaatt attttgtttc gtgatctgct aatgatgctc
      121 cgacaaatgc tttgattaat ttttgcgggc gatttggacg agaaatcaat tctgggtgga
      181 attggcatcc cacaaagaat tttttctctg gcagctctac gatttctact aaacggttat
      241 ctggtgagac acctgaaaag actagccctt gttcttcaaa caattgacga tatttgttat
      301 tgaactcata gcggtgacgg tgacgttctt gcacaacatc ggctccatca tatgcttcag
      361 ccgtcttcgt tccttttttg attttacatg ggtataatcc aaggcgtaaa gttccaccca
      421 aattctcgat gttttcctga tcagccatta aatcaataat attgtttgct gtatctggat
      481 tcgtttcagc tgatgacgca tcttctaatc caacgacgtt acgtgcaaat tcgacacaag
      541 ccatctgcat accaagacaa atacctaaaa acggaacgtc attttcgcga gcataacgaa
      601 tcgcttcgat tttcccttct actccgcgat caccaaatcc accaggaacc aagattccat
      661 ctgcgtcttt caaacgttct tctacatttt ctctcgttaa ttcttgtgct ttgatccaat
      721 cgatctcgat gtctgaatta taggcgaaac cagaatgttt caatgcttca actactgaga
      781 tatatgcatc tggtaactcg acatatttac cgacaagcgc aatccgtact tttttcttca
      841 agttcaagac acgttcttct aattctttcc attctgtcat gtctgcttct ggtgtatcta
      901 gttttaaatg gtcgcaaaca atttgatcca ttccttgtgc ttgtaaaact aatgggatgg
      961 agtaaagtgt atctacatct ggcgactcga taactgcttc tggttcgaca tcacaaaatt
     1021 gtgccaattt gtttttcacg ctttgagaaa caggttgctc tgtacgtacc accaaaatat
     1081 tcggttggat acctaaacca cgtagctctt ttacactgtg ctgcgttggt ttagttttca
     1141 tttctccagc agctttcaag taaggaatca atgttgtatg gatatacata acattatctg
     1201 taccgacatc tgctttcatt tgacgaagtg cttcaaggaa tggcaaagat tctatatccc
     1261 ctactgttcc ccctacttct gtaatgatca catctgaatc agtcatttta gctgctcgca
     1321 tgattttttc tttgatctca tttgtaatat gaggaataac ttgaacagtt gctcccaagt
     1381 attcgccctt acgttcttta cgcaaaactt cagaatagat ttttcctgtc gtcacgttgg
     1441 agtatttgtt caaattgatg tcaataaaac gttcgtagtg gcccagatcc aaatctgttt
     1501 cagcaccatc atctgttacg aatacttctc cgtgctgata tgggctcatc gtccccggat
     1561 ccacgttgat gtatggatca aatttttgga tagttacttt taagccgcgg tttttcaata
     1621 agcgtcctaa agaagctgca acaatccctt ttccgattga tgaaacgaca ccgccggtca
     1681 caaaaatata ttttgtcata agttcaaaaa ctccttttta atttatgtgc aggaaaagtc
     1741 tcagacaata aacaaagctc cctattcttc aataagaata gggagccgaa atttctgtaa
     1801 acttctgacc ctctaaaagg gtgcccaagg tgtataatac aagctgacta agggaaagtc
     1861 aagtactcaa atgaatacta tccgcttaca attttttctt tttcgatatc ctttgagaaa
     1921 ttatttctta agtcctttat gagtgcaaaa aaaatttaac ccacataata agcttgatat
     1981 aaacatagct aacgttaaac ctaagcaaat cagaaaatcg ttactgaaaa attttaagtg
     2041 aaataataga taaaaaaata aaacaaacat tatgatagat aatatcccac ctaacaaaga
     2101 actacttttt cctacttgca gcaacagttt atatagctta tcgtagaaac aatctttttc
     2161 tttcaccctt atttacctcc tttacgtaaa aaacgtagtc cacagttctc ggatttttgg
     2221 catatctttt ctattaaata atatcaaaaa caaaacaatt ccactaattt tttcaaagat
     2281 gacctttttt tcaacaaaag tgccttgtag ggattcattg aaggtaacga atcacataag
     2341 taaaaaataa ggagatgcga caaaagtcgt gcctccttat ttctaaataa acggtgttct
     2401 cctgccaact cctcggtcac aagtcacaat atccatcaaa catgagaagc tgtttttgaa
     2461 attatttctt gtgattcgga gttaaatggt aggctcacaa cctctggata aacggtattc
     2521 aaaagccagt ttctcggtat taagccaaat ttatgcaaaa atgtagaaag ccattttcac
     2581 aaaatttttc ttaatactcg aaactaaacg tctttttcaa aacctcttgg taaacggtgt
     2641 tcaaaagctt gcatctgcgg taataagttc agccaattca aaaacaagaa aagctgtttt
     2701 tgcttggcta ttcttattac ttgttgcagg gcagcttttt cacaacctct ccactaaacg
     2761 gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
     2821 tttccgttag tttctctaat actcaggtca aaacgctgat ttcacagcct ctaattcacg
     2881 gtattcctgt tttaataaag ttctagatat tgttctcttt cccactcgga aacagtttgt
     2941 ctgaatgccg cccattcgag acgttttgct tcaacaaaat ttgagaagat atgttgtcct
     3001 aatgcgtcaa tcattacatt atccttacgc aattctttga tagcattatg gatagttgaa
     3061 ggtaagtcct ggatatgtgc agcttctctt tcttcctcat ccattacata aatgttacgg
     3121 tcaacagctg ctggcggtgt gatctgatta cggataccgt ctagtcctgc ttgcaacaat
     3181 actgccattg ccaagtatgg gtttgcagat ggatcaactg accgcaattc caaacgtgtg
     3241 gataaaccgc gagattctgg tacacgtact aatggcgaac gattacggcc actccatgcg
     3301 acataaacag gcgcttcata acctggaacg agacgtttat aagaattgac tgttggattg
     3361 caaacggctg tgtaggcacg agcatgtttc agcaaaccac ctaagaaatg ataagctgtt
     3421 tcacttagtt ccaattcacc gtctttatca tagaaaacat ttccttcatc attgaacaaa
     3481 gacatattgc aatgcatacc agaaccattg atcccaaata acggtttcgg catgaaagtc
     3541 gcgtgaagtc catgcttgcg tgcaatcgtt ttgacaacga gcttgaaggt ttggatatta
     3601 tcacaagcat ctaccacatc tgcatattta aagtcaattt catgctgtcc tggcgctact
     3661 tcatgatgtg aagcttccac ttcaaagcct agactttcta attccaacac gatgtcacgg
     3721 cggcaatttt cacctaaatc tgttggtgca aaatcaaaat aaccaccttt atcattcaag
     3781 gtcgttgtga tcccaccgtc ttcgtctaat ttaaataaga agaattctgg ttcaggtcct
     3841 aaattgaaag aggtaaaacc aaggtcgcgc atatctttca atgctcgttt caaattcccg
     3901 cgaggatcgc cggcaaatgg tgtcccatcc ggattataga tatcacaaat taaacgagca
     3961 acttttccat gagcactttc ccacgggaag atcatccaag tggagagatc tggatacaaa
     4021 tacatatcgc tttcttcaat tcgaacaaat ccttcaatcg aagaaccgtc aaacatcatt
     4081 ttattttcta atactttgtc gatctggctc actggaactt ctacattttt gatcgtacct
     4141 aagatgtctg tgaacatcaa tcgtaaaaat cgtacgtttt cttcctcaat gattcgtttg
     4201 atttcttcac ctgttatctg tttctttacc atggtatccc tcttcctttt ttaatttatt
     4261 cagtcaaaga attgtctttg caatccttgc gactgataat gacctgtttt gcttaaccca
     4321 ccttgcgcca aaatctcatc acgaaaaatt tgtcgaacgt catgatctgt caacggcttc
     4381 ttgttttttg cttcttgttt tttcgcctgt gccatttcgt atacacgttt gatcccagcc
     4441 atattcaatc cctctgaaag atagtcttta atctcaagca acatatcaat atcattcagc
     4501 gaatacatac gtcggtttcc ttcgcttcgt tccggatgaa ctaaatcttg ttcttcataa
     4561 tagcgaattt gccgagcaga caaatcagtc agcttcataa ctgtaccaat tggaaatacg
     4621 gacatagatc ggcgcaattc cttttctccc atcagacgtc ccctttcgtt ttataagttg
     4681 gctaaagtgt accaattaaa aaaaatattg tcaactgcat atgttaggtt ttctaacatg
     4741 agatctctat tttttataaa ataacaaaaa aataagaaca caaaaaaggt atctagttga
     4801 ttttcgtacc agataccttt tattttttat tatactgtac tatcctttat tagtgttttt
     4861 att
//
LOCUS       DASJTC010000135         4794 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.139, whole genome shotgun sequence.
ACCESSION   DASJTC010000135 DASJTC010000000
VERSION     DASJTC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.139"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(80..1888)
                     /gene="pepF"
                     /locus_tag="U4B19_002606"
     CDS             complement(80..1888)
                     /gene="pepF"
                     /locus_tag="U4B19_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEN2078272.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2064..3062)
                     /locus_tag="U4B19_002607"
     CDS             complement(2064..3062)
                     /locus_tag="U4B19_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEN2078273.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3288..3941)
                     /locus_tag="U4B19_002608"
     CDS             complement(3288..3941)
                     /locus_tag="U4B19_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEN2078274.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="U4B19_002609"
     CDS             complement(4273..4671)
                     /gene="spxA"
                     /locus_tag="U4B19_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN2078275.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1405 a         1007 c          744 g         1638 t
ORIGIN      
        1 aaaaaatacg tcgactccct tacaagagag ccgacgtatt tttttcgttt ctattcgaga
       61 aaatcaaaaa tagaaagcct tataaacgat cgatcaattc ttctaattca tttagacgtt
      121 gttcaaacat cgacattgca tcttcaatat atgcagcctg tgtcatatca actcctgctt
      181 ttttcattac ttccacagga taatcgctgt tacctgcttt taggtaagcc aagtagttct
      241 ctagtgcctc tggttcctgg tttagtattt tcttcgccag tgctgaagca gcagaaaagc
      301 cagtcgagta ttggaaaaca tagtaattat aataaaaatg cggtatccgt gaccattcga
      361 atttaatttc tgggtcttct tcgactgctg gaccataata ttttgcattc aatttaccat
      421 aactatcact taggtattca ctagtcaatg gcacaccttt ttcatcttcc gtatgcatga
      481 aatgctcgaa ttcagcgaat tgtgtctggc ggaaaactgt tcctttaaac ccatccaagt
      541 aatggttgag tacataagcc cgtacacgag ggtctttttc tgtttccaat aaatactccg
      601 ttaggatatt ttcattcgtt gtcgaagcaa tttctgctaa aaagatggag tagtcgccgt
      661 acacataagg ctggttcgaa cgagtgaaat aactatgaac actatgtccc atttcgtgga
      721 caagcgtaaa tagctgatcc agcgtatcat gccaattcaa taaaatatat ggatttgtgt
      781 catagcttcc cgaagaataa gcaccgcttc gtttcccttt attttcgaca acatcgatcc
      841 aacgttcaga gaatgctttt tctacgatgg ccatgtattc ttcacccatt ggtttcagtg
      901 cttctaaagc tttttctttt gcttcttcgt acgtaaaggt aattggagct tcccctaaga
      961 ccggtgtata aagatcatac atgtgcagtt tttccacttc taataaacgt ttccgtaatt
     1021 ccatgtatcg atgtaacaaa ggcaaatgct tgtttaccac gtctaccaaa gtatcgtata
     1081 cactttcagg aatatgatta ttgctcaaag acgcttctct ggcagagctg taattacgga
     1141 ctttcgcttt aaaattatgt ccttttatat gtgtgcctaa agtagaagca aatgtatttc
     1201 taaattgttc gtaaacactg tacaacccct taaatgctgc ttcgcgcacc cttcgatccg
     1261 tgctttctag caactgacca tacacgccat gagataattg gactatttca ccattttctc
     1321 cttcaatcgt tggaaaaact agatctgcat tattcaaaac agcaaatgta tcacttgaag
     1381 catcaaagat ttcacctgct ccagcaagaa gagattcctg ctcagcagat aagacatggg
     1441 ctcgattatc tactatttgc tggatataat ggcgatagac ttccaatttt ggttcttctt
     1501 tgaaatattg ccaaatctgg tcatctgaca attgcaatat ttctggttca aaccaggaaa
     1561 cggcttcact aactttcgaa aacagactgc ttgctcttgc ataaagcgct tggtaatctg
     1621 tatttccagt gtcttgatcg tttttaaggt gcgcatatac ataaatgact tcagtttggc
     1681 gataaaccct caatacgaat tcaatcgcat ttaaaaattg agaagcgcct tgatcaagtg
     1741 ttcctttgtg tttttcagat tgttttaact cttctgataa ttccaaatat ttctcatcaa
     1801 actcttggtc gcttgaaaag atcttggtca agtcccaagt taaattttca ggcaattctt
     1861 ctcgttttgg caactgcttt acttccatga aatccactcc ttttcttccc ttagctctat
     1921 ctatcaaaat agatcatagt ctaacatcca catgtcacag catctaactt ttctactcat
     1981 tcatgtgtct gcttccatca attttagcat aactaaatct tgttttacat agaaaaccga
     2041 ttcctagcct atttttatac ttactatttt ttcaaagaag acaagtgtat gcattcgtta
     2101 aaaaactgct gatagaccat ctcttcatca atcataggaa aaagccattc ccttttatgt
     2161 tcttttattt ggcaaagcca tgagttgaaa tcaggaactt tctggttttc caaggcttct
     2221 tcgtacaaca ttcgataaag aaaaacctgt tcttgtaaat aaaagaaaaa ccgacttttt
     2281 tgatagatcc acgatgatag atgcaaaaga tgtttatgac gcaagtagca ttgttcttgt
     2341 atggatactg tattcttttg cttgtgaata agttggcgtg tcagccattt cttcccatct
     2401 tcactcactt tttctatttt tttcatttgg ttcattttaa tttcgttact agtgaatatt
     2461 tctttaaggt ccctactaga aaaagtccag cagctactgc aaaaggaaat atgccctgat
     2521 acagtctctg taatatgatg atacaaataa agcttttgtt tcttgagatc agctttccag
     2581 caatgcactc ctctttttct attatagtag cagcaacttt tttctatcaa tgagaattgc
     2641 gattgtccaa gaaaagaatt accaagtatc caccatactt ggtaactgtt attcaaataa
     2701 ttttgggttc tttcaataaa tctctggtga gacagtctag aacattggat ttctattgct
     2761 agattcgcca acaaaatatc cggcctttgc cgcaacctcg gcaaataggc ttccaaaaat
     2821 actggttcta ctgactgtcc cagccaatcc atgaagcatt cctttaactg taagtgctct
     2881 tttgattctc tttcactaaa gaccgaacag ttatctgatt tttgatgggc aaaatgagca
     2941 cattttttct ttcccttttt caaaatcact ctttttcgac agccacgaca ataatatttc
     3001 tttccttttt ctgcatgttt agcaaaacag cacttattat attcattcat tgcaagcagc
     3061 atttttctcc ctctgaactt atttacgtct aagaatttat ttacacgaaa aaagagggcg
     3121 tgaaaaagct attctgcatc aagtaataag aacagctaaa caaaaatagt tcccaatatt
     3181 tttcgtttgg ttgggcttgt taccgcagat gcaagctttt gaacacattt atacgaaaaa
     3241 aagactgcga cacaattttt gtcacagtct cttactataa tgctgattta tacaaaataa
     3301 aatttcgttc gctcgatagc attacgcgcc atcagacatt gtccatattc attcaatgtt
     3361 tctggtgtac gatcgctctt ttcagcaaac tccaaaattc gagcaatctg attctctaca
     3421 tccgcttggt tgagattttc catccagaag tgaacgacta ggtaatagcg ctcttcgaat
     3481 aaatacaaat cagtccaagc agactctagt tcgacatctg ttgcaagttg gatcacttgt
     3541 tcaaaatctt caaaagcaaa agttgcttgt ctttcctgtt cttctagggc gcttccagat
     3601 aattcttcct ccggatcgct gccaataatc tgctgacgaa gaaagtcact cacatcttct
     3661 gaatcaacag tagaatcttc tgattgagaa atttcttcat ccggaagatt tttactgata
     3721 aacagttcta atccgtctcc ttttggcaaa acttggaaag tgacagcttc gcttcctcgg
     3781 aactcctctt cgatatcaac ttcttccaaa atactataaa agaaattttc gatttctttg
     3841 tgattgccaa gtaaatccaa aaatgaaacg ccccgttcct ctaaatcttc atgaccaatt
     3901 agtacacgga tcgtattttc attaatatgt tccatttcca ttttgctaca cctcactttg
     3961 ccacaatgtt taatatagat tcattgtagc gaaatcagct taaatgtaaa gaaaaaaaat
     4021 ctcgctgtta agcgaattta ataaaaccag cggatttttc aacctttcaa gctatcttat
     4081 ttaactataa catttttggc agacgaatac tatatagccg acttttcagc taattttccc
     4141 tctctatttt tcgtgcaatc atcatcttct actacctttt tatacatcta atcaagaata
     4201 ctttttgtaa aaacaaaaaa acttggaaaa actatctttg taatagtctt ttccaagttt
     4261 cgtattatcc ttctataatc cagccatcaa ttgtgcttga cgtaattcta attgtcgtac
     4321 atcgcgcggt aggaaccgac gaatttcatc ttcattaaaa ccaacttgca gacgtttatc
     4381 atcaatcatg attggacggc gaagcaatcc aggattgttt tgcacaagtt ccaataattc
     4441 ttgtaacggc agatcatcta aatccatgtt cagtttttgg aatactttgg aacgtgtcga
     4501 gatgatttct tctgttccgt cttctgtcat ttgtaaaatc gcttttaatt cagaactgtt
     4561 caatggttct gaaaaaatat tacgttcaac aaatggaatc tgatgttctt gcaaccacgc
     4621 acgagccttt ctgcaggaag tacaacttgg ggaagtatat aatgttaaca acgtgcacca
     4681 ctccttttcg tgataagaat cacttttttg taacagaaga tcataataat ctcccatctg
     4741 tttctattat acataatcaa cttttaaata gctacctttt tttcataatt tttt
//
LOCUS       DASJTC010000136         4725 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.140, whole genome shotgun sequence.
ACCESSION   DASJTC010000136 DASJTC010000000
VERSION     DASJTC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.140"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            174..404
                     /locus_tag="U4B19_002610"
     CDS             174..404
                     /locus_tag="U4B19_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEN2078276.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            501..1802
                     /gene="murA"
                     /locus_tag="U4B19_002611"
     CDS             501..1802
                     /gene="murA"
                     /locus_tag="U4B19_002611"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEN2078277.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1815..1991
                     /locus_tag="U4B19_002612"
     CDS             1815..1991
                     /locus_tag="U4B19_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEN2078278.1"
                     /translation="MSNQRYILLTLIKILMVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2126..2422
                     /gene="yidD"
                     /locus_tag="U4B19_002613"
     CDS             2126..2422
                     /gene="yidD"
                     /locus_tag="U4B19_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEN2078279.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            2607..3074
                     /locus_tag="U4B19_002614"
     CDS             2607..3074
                     /locus_tag="U4B19_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2078280.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3159..4043
                     /locus_tag="U4B19_002615"
     CDS             3159..4043
                     /locus_tag="U4B19_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2078281.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4157..4393
                     /locus_tag="U4B19_002616"
     CDS             4157..4393
                     /locus_tag="U4B19_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEN2078282.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4481..4561
                     /locus_tag="U4B19_002617"
     tRNA            4481..4561
                     /locus_tag="U4B19_002617"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4515..4517,aa:Tyr,seq:gta)
     gene            4568..4639
                     /locus_tag="U4B19_002618"
     tRNA            4568..4639
                     /locus_tag="U4B19_002618"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4600..4602,aa:Gln,seq:ttg)
BASE COUNT         1583 a          742 c         1032 g         1368 t
ORIGIN      
        1 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
       61 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
      121 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
      181 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
      241 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
      301 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
      361 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
      421 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
      481 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
      541 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
      601 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
      661 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
      721 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
      781 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
      841 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
      901 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
      961 tgatcggtaa cacgatttat ttagatttcc ctagtgtagg tgctactcaa aatatcatga
     1021 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
     1081 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
     1141 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
     1201 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
     1261 ttgcagatgc tatctctgaa cataaccgcc cattgatttc taaattgatc gaaatgggtg
     1321 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
     1381 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
     1441 ctatccaatt agttgcagaa ggtacaagtg tagtgactga aacagtgttt gaaaatcgtt
     1501 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
     1561 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
     1621 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
     1681 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
     1741 acgttgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
     1801 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttaa
     1861 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
     1921 tcggtggcgg caatccattt aaagtatttc agccaagttt gtggattcat attcgagact
     1981 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
     2041 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
     2101 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
     2161 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
     2221 ttatatgata aaagcaattc aaacccatgg cgcgctaaaa gggacggtaa tggggactgc
     2281 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
     2341 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
     2401 tacgggtagc actgagcgat aaagaaacaa tttggaaaat agcttcttat attttgcaaa
     2461 attgtgagtt attgccgaag tgttggattt tgaacaccgt caaaaaaaag gttgtgaaaa
     2521 atacggctag cactgagcga taaagaaaaa gaggaaattt attgtcaaag taacaactcc
     2581 gcaactactt atatggggga caaaaaatga tcgaagaact aaacaagaaa gatttaccat
     2641 ggtccttgtt attagatgca gatcctgata aagaaaaagt tcaagtatat gtttccagag
     2701 ggagtggctg gatatggaaa gaaaaagaaa aaacaatagg tgtactgatc tatgtggcaa
     2761 gggaaaaaga atacgaaatc gtcaatgtag cagtaacccc aacgtatcaa ggaaagggga
     2821 ttggcggaaa attacttgag acagcttttc agaagctttc acaactcgct tccagtcaaa
     2881 ctcagatcat catacgaacc ggcagtacta gcagtgcggc gttacatctc tatcaaaaaa
     2941 tgggctatgt ggagattggt cgagtcaaag actattttat ccatcattac tctgagccca
     3001 tttttgaaaa tggagatcaa ttaagggatc aagtgatttt agcaattcac ttacctaaaa
     3061 atagctttaa ctaataaaat ttcaattcaa gaaaatgctt tcaaatgcta aatagataca
     3121 ttacaatagc tttagcaaac aaaaaggaga atatttttat gggaatatta gtttttgatt
     3181 ttggtggatc ggctgtcaaa tatggttgtt gggatggcaa agagattaaa ggtaagggac
     3241 aatttgcaac gcctgaatct tgggaagaaa tgaaagcgca attatttcaa gtttataaaa
     3301 aaatactcgt ttcttttgaa gtcgaaggaa ttggaatcag ctctcctgga gtagtgaata
     3361 atgagaaaca ggtgattgag ggaatcagtg cgattcctta tattcatgga tttaatattt
     3421 tccgagattt ggaaactctg tttcaacttc cagtaacgat tgaaaatgac gccaactgtg
     3481 ccggaatggc tgaattttat gaaggtgctg cccgcgatta tcaagatgtt gcttttgttg
     3541 tggcaggaac tgggattggc ggggcgttgt tcacgaaagg acaaatcaat aaaggggcac
     3601 atctttacgg tggagaattt gggttgatgt tcttaaatgg cgataagaca ttcagtaaac
     3661 ttggtacagc ggtacagatg gcttggcgtt attgtgaacg caaaggtctc gataaaaatg
     3721 cttataccgg aaaagatgtc tttgaattag cagaaactgg agatgcaata gcaaaagaag
     3781 aagttgagag tttttatacg tatcttacaa agggattatt cagtattcaa ttttcgtttg
     3841 atccagaagt gatcgtatta ggcggcgggg tttctgcaaa agaagggctc attgatgaga
     3901 tcaaaagccg aatgaagaag ctgacagaac aattcgatct gcatgatttc gaaccgcaga
     3961 ttttattatg tgaatacaga aacgatgcta atcttgtagg tgcagcagca aactatctcg
     4021 ctgtacgtca aggggagaat taagagcttt agagaaggtg cagcttgcat aaaagaagtg
     4081 aatgaaatga ataggagatt tttacctaag gaatgtttta tttgctgcag tatgataaaa
     4141 tatctgagag gtgaaaatga tgtcaaaaaa aaataaagat attgaagtgc gtatagaaga
     4201 agtgaaaaaa gatatcaaag gtaagaatta tgaagtcact cagttattta tagggaaaaa
     4261 aatgattggt gagatcttag cctatggacc aaaagaatat gaaattttct ttggtgaaga
     4321 agattttgga aaagaaaaaa gtcttgaaaa tgcgatagaa acagtgattc gacactggaa
     4381 tctgcacgaa taaaaaaata tcaaaaaaac tttcaataaa gggttgcaaa aatctggaaa
     4441 attaggtatt ataactaagt caggttgata caaacctttc ggaggagtag cgaagtggct
     4501 aaacgcgacg gactgtaaat ccgttcctta gggttcagtg gttcgaatcc actctcctcc
     4561 atttcattgg ggtatagcca agcggtaagg caagggactt tgactccctc atgcgttggt
     4621 tcgaatccag ctaccccagt ataccacaat cgtaagctct cgttagttcg agagctcttt
     4681 tttttgcttt gctaccttgt tccttgaaga ttctgctgat ttaag
//
LOCUS       DASJTC010000137         4683 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.141, whole genome shotgun sequence.
ACCESSION   DASJTC010000137 DASJTC010000000
VERSION     DASJTC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.141"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..965)
                     /locus_tag="U4B19_002619"
     CDS             complement(<1..965)
                     /locus_tag="U4B19_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEN2078283.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRIS"
     gene            complement(1036..1332)
                     /locus_tag="U4B19_002620"
     CDS             complement(1036..1332)
                     /locus_tag="U4B19_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078284.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1325..1618)
                     /locus_tag="U4B19_002621"
     CDS             complement(1325..1618)
                     /locus_tag="U4B19_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078285.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1764..2315)
                     /locus_tag="U4B19_002622"
     CDS             complement(1764..2315)
                     /locus_tag="U4B19_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEN2078286.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2489..2788)
                     /locus_tag="U4B19_002623"
     CDS             complement(2489..2788)
                     /locus_tag="U4B19_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HEN2078287.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(2815..3123)
                     /locus_tag="U4B19_002624"
     CDS             complement(2815..3123)
                     /locus_tag="U4B19_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEN2078288.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(3240..4199)
                     /locus_tag="U4B19_002625"
     CDS             complement(3240..4199)
                     /locus_tag="U4B19_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2078289.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            4343..>4683
                     /locus_tag="U4B19_002626"
     CDS             4343..>4683
                     /locus_tag="U4B19_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEN2078290.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEF"
BASE COUNT         1358 a          888 c          730 g         1707 t
ORIGIN      
        1 tgagaaattc ttccatctgt ataaacacgg aattgttttc caccagccat tgttgtattt
       61 ccattatcat ccgttacaat attaattgaa tagtagcttc cattttcatc ttgcattaga
      121 tctttcatat attcatttgt gctaattaaa ttagtatttg atgcattttt atcaggagaa
      181 tcatacaacc ataaagcttt attatttagt acagtttctt tagcagcttc ccaacttgaa
      241 attgctgagc tacttgtaga ttgtgtcgtt gtagttgttg cttgttgagt agattcttct
      301 actactggag tttgaggagt ttgtccattc acaatatcaa tgaaaccttg ttttaactgt
      361 tggttctgag tttcattgaa gtacatgtag tttttatcat tgccttgatt ttgttgcgtt
      421 aaataaactt taggttgtcc attttgaaag ccaaagaaat atacatgctg ccctaaatat
      481 tctccagttt ctacatcaga ataaactgct actaaattga aatctgcatg accagttcca
      541 tcttctgacc attgtacggt aactggtgct tcattgattg ccattttcca atttccatca
      601 ataactgcac ttggtaaagg cgtaccatac agacttacat tgttctgagg attgtagctt
      661 ttatattctt gacctaatgt ctttccccat tgtgggacaa aaattgctag ttgagtagat
      721 ttctcactat cccacaaagt atttttaacc tcttcagaag aagattcaat gattgaactt
      781 tcacttgtac ttgcttccac agattgtttc atttctgttg tagtttgttc agttgattgc
      841 gttttacttt ttttagcctc aattgttgta tcagtttcta cagtctcttt agtcctcgta
      901 ttctcagatg tacaaccagt gaataaaatc cccattccaa ttagtaataa caaccatttt
      961 ttcattttat ttcccccttt caatttctcc ctcgcgtact tattttatca gtcaattaat
     1021 ctcaatggtg ttcatttagt taatcctttc ccaagaatgt cgatagccag catgacaacg
     1081 atctcttcct tgagggcttt cagatggatt aaaactagta gaaatttccc ttccacatcc
     1141 tctacagcga taagttctca ttatttttgg gctattagta ttatttttct tagttagagc
     1201 tatgagtcca actatgatta ccccaactgt cttaattact tttcctttat ctatattcgg
     1261 atgcgcaagc atatatttat ataaccaata tccaccgtaa attaaaccac ctaatacaat
     1321 cagcttagcc atgtactcgt ctcctttctt tttgtttaat tactttttga atccatcttg
     1381 cagagaaacc ataaacttct gtcaattgac ggatatctac agttttttcc tgttctatca
     1441 tgttttcgat tctttttcta actaactcac gatcgtacat tttcattgga aaagaaatct
     1501 gttgccctcg aaaagcaaga taaaattgat agagcaattc tgaatccagt tccatttcct
     1561 caaaaaagtc attcatactt ttataagagt tactcaaaca agtttcatca atctccatct
     1621 acacaccccc tgttattttt tacataatga aaatagcata ttactgggta cattgataat
     1681 tcatataaca tgtatcatca catgtatttt taaagtcaaa aagcatgtaa aactacttat
     1741 aagtaacata agaaaataaa atgttatttt tcttgaaata ataattgact atctgccaat
     1801 tgccattcat tacgaacatg aatacttagt gggtgggata acttaccttc tgcatttatc
     1861 aatactttgg tcatcttttt ttgtttcgca ctcaattgat cgtaaatggt cttcgcttgt
     1921 tcataagcca cttgactcgt cgctgtaatc gaaccacaag caaaaataat ggtgttgatt
     1981 ttcttttctg tgaaaacagt tgttaatatt tccaaagatt gttcatcatt tccacgagga
     2041 aatacccgag gacttttcgc ttttgtatac gaaaaaagat tcacagcaat aaaaccagta
     2101 aatcctaatt gtcggatatt cttttcgatt aataacatac tcaaatcatt cgtaaatggt
     2161 tctgtatctg ttggacgaat agtaatgact accgctaatt tatcttccgc ttcttttcct
     2221 ttgatcgccc attgtcgttt gaaaacatag cgatgggtat gatgaggatc tcgccaaact
     2281 tctgtttgtg tggtcactaa ttctttttca atcatctctt tttctcctat gctaattttt
     2341 gttcaagcaa ttcaatcacc attgccgtta taagtgtcaa tactagttga aaatatttca
     2401 tattctttct ccttttttct tcagaatttg atgaatcgct gtaaggatat cttctaaaca
     2461 tttcgcaatc attctcaaat aatcgatctt attcattcgt tcctccaatc tgactccaaa
     2521 ccaatcgtcc ttctaccagt aacccacgaa caaattctcg ctggctggta atatctgtaa
     2581 tgaaataaac tacatagtct tttccatctt ctggtgcgca ataaatataa agatgtgtca
     2641 tcactaaacg tggtagagta acatcttcag gaagcatacc catttcaatt tcctcttttt
     2701 ccatcgtaac ggattcatag ccccactctt caggagtagt gactagctgt gcacactttt
     2761 ccagtacttc gatagccgtt tctaacaagt cctttcgttc ctttctttta gatatcagta
     2821 ttcttctggt aataacatcg tttgatactc acccgaatca atgacccata ttttttgaga
     2881 cactgatttc aaacgaagct taggtgaaat cagatactct tttcttgctt ctggttcttc
     2941 ttgacgatga accaactttt gatttccttt ttccgtagaa aattcaaaga tttgaagata
     3001 gtctaaatga aattgctttt gaacgttttg atcgattaga ttccaacata aagcttgtaa
     3061 ttccaccggc aaactgtcca ttactccctt tgtgacataa cgttgtcctt tattttgaaa
     3121 catttctttt ccctcctttg ccataaaaat agcccaatct tctattaaag aagattgggc
     3181 ttcatggata taatatatat ttgatattca atttgattca ttttatacta tctgatttgt
     3241 caaattaagc tagacaatat gtctttatct atgcagctca aaaatacttc caatggtgtt
     3301 cgataattta atgattttct tggaatatgg tttcttttcg aggcaacgga ttgaataaag
     3361 gtttcatccg tgtccctaaa gtccattgtt ttaggtaagc catctcgtct aagcaagccg
     3421 tttgagtgct catttagccc tctttgcgaa ggtgttcctg gatctgcgaa aaagatggca
     3481 atatcgttcg agttacttat agatcgccag ttggaaaatt cttttccaca atcaaatgtc
     3541 atggatttaa acaagtgttt aggcatggat ttgaaccaat catttaacct gttttcgata
     3601 tcttcggctc gtctgcctgt cggtttcaaa gtaataataa ccttggataa acactcaacc
     3661 aatgtaataa cagcgctctt gtgtttaact cccacaatgg tgtcgacttc gagatgacca
     3721 aattctttag tcaggtgtgg atagagactg ttccgttcat gaatcgttct gcgaaaggcc
     3781 tgttttccac gcttttcttg atgattattc ggtttacgtt tccccttcat aggtaacgac
     3841 tgtgtgtcaa aaaggccttt tttaaacatc cgatagagtg tacgaacaga acaatcaatt
     3901 ggaaactcgc cacggccgat aatcacatcg ggtgaccatc cttgagctac cttttcttga
     3961 acatatgttg attgttcatc agataaaacg gttgaacgtc gtccacaacg agatttattt
     4021 tgtttgtatt gacggtaata atctagcact ttttttcctt gtttgagaaa cgaataaaca
     4081 ttataaatgg tttgtctaga gcgatttaaa ctttgtgcga caagagcgac aggtttctcg
     4141 atattgaaat aagactctat gattactagt tcgtctgttg taagatgggt gtaagtcatt
     4201 tgtactcact ccttattaat tctttggtcg gaactatttt gagtgtagca caaatgatct
     4261 ttttagttgt ctagcttaat tttacaatcg acgttttaat aaattgtgat ataatgaagt
     4321 ataacttttt agggagtgta ctatgaattt tcaagataat agaccattat atttacaagt
     4381 agaagcttct atacgtaacg atattcgtga aaaaaaatat cttccaggag agcaattacc
     4441 tacagaagaa gaattatgta aaatttatgg agtaagtaag attaccattc gaaaagcttt
     4501 taaactttta gcagaaagtg gaacagttga acgattaagg gggaaaggga cctttgtaac
     4561 tcaaaaaaaa gaaagtttaa aattagctgg agttcatgga tttagcgatt ctctttctac
     4621 ccgaggacat aaaattagat atactgtttt acattctaaa attataaagg ctgatgagtt
     4681 ttt
//
LOCUS       DASJTC010000138         4590 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.142, whole genome shotgun sequence.
ACCESSION   DASJTC010000138 DASJTC010000000
VERSION     DASJTC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.142"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            186..2855
                     /locus_tag="U4B19_002627"
     CDS             186..2855
                     /locus_tag="U4B19_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEN2078291.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3566)
                     /locus_tag="U4B19_002628"
     CDS             complement(2946..3566)
                     /locus_tag="U4B19_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEN2078292.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="U4B19_002629"
     CDS             3680..4219
                     /locus_tag="U4B19_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078293.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="U4B19_002630"
     CDS             4216..4554
                     /locus_tag="U4B19_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HEN2078294.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1421 a          790 c          859 g         1520 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggagaaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgacatt
//
LOCUS       DASJTC010000139         4571 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.143, whole genome shotgun sequence.
ACCESSION   DASJTC010000139 DASJTC010000000
VERSION     DASJTC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.143"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(113..1048)
                     /gene="murB"
                     /locus_tag="U4B19_002631"
     CDS             complement(113..1048)
                     /gene="murB"
                     /locus_tag="U4B19_002631"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEN2078295.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1121..2041)
                     /locus_tag="U4B19_002632"
     CDS             complement(1121..2041)
                     /locus_tag="U4B19_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2078296.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2154..2906
                     /gene="xth"
                     /locus_tag="U4B19_002633"
     CDS             2154..2906
                     /gene="xth"
                     /locus_tag="U4B19_002633"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEN2078297.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3770..4303
                     /locus_tag="U4B19_002634"
     CDS             3770..4303
                     /locus_tag="U4B19_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEN2078298.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1348 a          897 c          812 g         1514 t
ORIGIN      
        1 tgttttattg tgttaaaatg tacaagattt gcgccatttg cgtaaacaac cacaaaaaag
       61 agcaggacgg ccaccgtcct gctcttttac ttattttaag ttccattact tattaattgg
      121 ctaaacttct gcttgtttct gatattgctt gtttttctcc aatgatccgc acttctgttt
      181 ctaaagacac atcaaatttt tctttgatta cttcttggat gtgcgcaatc aattcaatat
      241 aatctgtcgc tgtcgcatga tcgatattga cgatgaatcc agcatgtttt tcagagactt
      301 gtgcaccgcc ccatttcagg ccttgcaatc ctgcttgttg gatcagctga cctgtatagt
      361 gtcctactgg gcgcttaaag acacttccgc atgaagggta ttctaatggc tgttttgatt
      421 gacgcaattc ggtcaactca tccattcttg ctttgattgc ttcataatcc cctgtttgca
      481 aactgaatcg agcttccaat acgatagcac gtaattcttg gactttactg tgacgataag
      541 aaaataccat ttcgtctttt gtcaaagttt tcaatgttcc atcttcaagc aaaagatcca
      601 cttcagcaaa aacatctttg atctctccgc catacgcacc tgcattcata taaaccgctc
      661 caccgacact tcccgggatg ccgcaagcaa attcaaatcc agtcaatgat tctgccaaag
      721 ctgcataagt cgtatcaatc agtgaggcac ctgcttccgc aatgaccatc gttcctttga
      781 catgaatctg tttcatttca gtcagcatga tgactacacc gcgaatcccg ccatcttgaa
      841 cgatcagatt gcttgcattt cctaatacca tccaaggtat atcatggatt cggcaataat
      901 cgatgatttg ctttacttct tcttgttttt tcgggaatgc aaggacatca gctggtccac
      961 ctgttttcgt aaacgtatag ttcatcaatg gttcatcaaa caatatagta atcgtggcta
     1021 agctttcaac tatttcttct ttattcactg aggattttcc tttcttttct caaacgtcct
     1081 cattattcta tcacgcttaa tacaatcttg ccagtcttgt tcaaagttga tgacggtagt
     1141 gatctttaag cacttcctca cataattcat gacttcttac aacttctttt tgtttaagca
     1201 attctgtttc cgatttacct aaacggatct gagaaaagaa ggcctctacc attggctcaa
     1261 acccgcgttt gactagcgtg tttgtccagt cgccaaattc tttcactttt tttccatcag
     1321 catcttgaat gacaagctgg gtcagatcgt ttacttcata agtaccattt ttacttgtta
     1381 cttgatattt ttctatatta gcgccacttg ctaaatccat cgttaaaata gccgtttgat
     1441 tttgtgtttc taattgtaaa atggcatatt ccaaaaattc ctttgtttct cttatctggt
     1501 attttgttct caaaatgggt tcatctaata aatacacagc tgtatcaata agatgtaaaa
     1561 acaaatcata aaggacgaac tctgtcgttt cttttgccgc tattcgattt ttttgcaggt
     1621 ggatcaaccg cttatctggg atacgtttca attcttctac cataggagca aagcgccggt
     1681 tgaaaccgat catcaaaatt atttggttct tatcagctaa ttcttgcaat tcacgaactt
     1741 catttagatg gacactcagt ggtttgtcaa tataaacatg gataccattt tctagacatt
     1801 ttttggctaa ttcataatgt acttttgttg ctgcgtggat catacatgct tcgatttttt
     1861 cttctaaaag ctcatcgatc gtttcatata gatgttggaa cccgtactct ttttgaattc
     1921 tttcttttgt ttctttattt cttgttgcaa aataaaaatc aagtgttcct tgttttttcg
     1981 cataagttgg taaatatgct ttttctgcga tattccctac accgattaca ccaatcttca
     2041 tctactttcc tcctggatca tttagttact ttcatcttac accatttttt tctaaaatga
     2101 ggtacaatag tatcgcaaag gtaaggaaat taattcgaag gagtgtgctt ttattgaaat
     2161 ttatctcttg gaacgtaaac gggcttcgtg ccatcgtaaa taaaaatttt ttagaagttt
     2221 ttcatgagtt agacgccgat tttttctgtc tgcaggaaac gaaactccaa gcaggacaaa
     2281 ttgatttaga tcttccaggt tattatcaat attggaatta cgcggaacga aaaggctatt
     2341 ccggcacagc gatttttgct aaaaaaccag cattgaatgc cacttacgga atgggtatcg
     2401 atatccatga tacagaaggc cgtctgatta ctttggaata ttccgatttc tttcttgtaa
     2461 cctgttacac gccaaattct caaagcgaat taaaacgttt agattaccga ttagaatggg
     2521 aagaagcctt ttataattat ttggaaaatc taaaaaaaca aaaaccagtc atcgtgtgtg
     2581 gggatctaaa tgtcgctcat caaaagattg atttgaaaaa ctggaaaacg aatcaaaaaa
     2641 atgctggatt cactccagaa gaaagagcgg ctctttctcg actgttggat aacgggttta
     2701 tcgatacatt ccgttatttt tatccaaccc aggaaggtgt ctactcttgg tggaactacc
     2761 gattcaattc aaggaaaaac aatgctggtt ggcggatcga ttactttttg acgagcaaag
     2821 atttagagcc aggactagct gatgcaaaga tccatacgga cattatgggc agtgatcatt
     2881 gtccagtaga attagatttg aaataaagag gttgtgaaaa agtcgtttag tttcgagtat
     2941 taagaaaaat tttgtgaaaa tggctttcca cattttgcat aaatttggca taataccgag
     3001 aaactggctt ttgaacactg tttatcgaga gattgtgaaa aagctgtcct gcatcaagta
     3061 ataagaacag ccaaacaaaa atagttcctc atatttttcg tttggttggg cttattaccg
     3121 cagatacaag cttttgaaca ccgtttatta agaggttgtg aaaaagctgc actgcatcaa
     3181 gtaataagaa cagccaaaca aaaatagttc ctcatatttt tcgtttggtt gggcttatta
     3241 ccgcagatgc aagcttttga acaccgttta ttaagaggtt gtgaaaaagc tgcactgcat
     3301 caagtaataa gaacagccaa acaaaaatag ttcctcatat ttttcgtttg gttggactta
     3361 ttaccgcaga tgcaagcttt tgaacaccgt ttattgagaa gttgtgaaat cagcgtcctg
     3421 acctgagtat tagagaaaca aatggcaaaa tagttcctca tattttgcta ttttttgagc
     3481 taataccgag ggtcagctga ttgaacaccg tttagtggag aagttgtgaa aaagtcgcct
     3541 actttcgagt cgtaagaaat ttttttgaaa atggttcctc acaactttgc ataaatttga
     3601 cttaataccg agaagtcatg atccccatct aactccaaat cacaaggaat gagttcaaaa
     3661 actagctttt ctcaacaaat attacataaa aaattctatg ttttttgtta aatttatttg
     3721 cttttttcaa aagtatgtta cagtaatttc ccaaggaggt tttacagaaa tgaattttgt
     3781 tgccatggat tttgaaactg ccaatcatca gccttatagt gcttgttctc ttgcgctagt
     3841 catggtaaaa aatagccaga tcgttgatga attctataca ttgatacagc ctgagactcc
     3901 ttttttctgg cgaaatgtcc aaattcacgg aatccatcaa gaagacgtac gcaatgcgcc
     3961 gaaattccca gaagtctggc aaactatcca accatatttt caaaaaaatc gtttagtcgt
     4021 tgcacataat gctgcttttg atacaaaggt cttagcggga tgcttagatt attatcatct
     4081 tgaacagcct aattatcttt ctttatgtac agtgaaaagt agccgtcgcc tgtttcctga
     4141 atttccaaat catcgattga acactgtgtg cgaaaatttg aacatccaat tagatcatca
     4201 ccatgatgca ttggaagata gtcgtgcatg tgcggaaatc ctgctttatc aagaaaaaaa
     4261 attcgggaca gatccattga aaaaacttgt gactataaag taaaagaata cttatgaaac
     4321 acacaaagag gtcgtgagcc tgccgtttca ctccgagtca caaggagcaa tttcaaaaaa
     4381 tagcttctca tgtttattaa atattgtgac tggtgaccga ggagctggca ggagaacacc
     4441 gtttattgga gaggttgtga aaaagctgtt ctgcatcaag taataagaac agccaaacaa
     4501 aaatagcttc tcatattttt catttggttg gacttattac cgcagatgca agcttttgga
     4561 caccgtttac c
//
LOCUS       DASJTC010000140         4553 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.144, whole genome shotgun sequence.
ACCESSION   DASJTC010000140 DASJTC010000000
VERSION     DASJTC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.144"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(12..482)
                     /locus_tag="U4B19_002635"
     CDS             complement(12..482)
                     /locus_tag="U4B19_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEN2078299.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="U4B19_002636"
     CDS             complement(641..1099)
                     /locus_tag="U4B19_002636"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEN2078300.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="U4B19_002637"
     CDS             complement(1270..2415)
                     /locus_tag="U4B19_002637"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEN2078301.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="U4B19_002638"
     CDS             complement(2428..3345)
                     /locus_tag="U4B19_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEN2078302.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="U4B19_002639"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="U4B19_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEN2078303.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1419 a          834 c          865 g         1435 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaa
//
LOCUS       DASJTC010000141         4492 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.145, whole genome shotgun sequence.
ACCESSION   DASJTC010000141 DASJTC010000000
VERSION     DASJTC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.145"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..895)
                     /locus_tag="U4B19_002640"
     CDS             complement(<1..895)
                     /locus_tag="U4B19_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2078304.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKK"
     gene            complement(897..2336)
                     /locus_tag="U4B19_002641"
     CDS             complement(897..2336)
                     /locus_tag="U4B19_002641"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEN2078305.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2521..4467
                     /locus_tag="U4B19_002642"
     CDS             2521..4467
                     /locus_tag="U4B19_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEN2078306.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1418 a          773 c          753 g         1548 t
ORIGIN      
        1 tttttttcag aagaatatca acaataagac ttgcaatttc atcaattggt tgtcttattg
       61 ttgtcaattg tgggaaaaat ttctcaataa aagaggtacc atcgtatcca ataattttta
      121 aatcttctgg gatattaagt ttaagttgat gagcaatttt catagtgaga atggctgtta
      181 aatcatcaga aacaaaaata ccatctggtt tgtgatttat aatagttgac tttatttcca
      241 tttcacgacg gatagtagac agattattag gaattttgaa aatttttcca tttggatttt
      301 gaaaagaaaa acctagtgca cgtaattctg tgggagaatc cgtgttatca tttcctgtaa
      361 tcataatggt attttgacaa ccattttttt gtaaagtctt agctgcaagt tttccccctt
      421 caaagttatc tgaagaaaca atggaaacat taggagctaa atttctatca aaagcaacaa
      481 taggtgctct cacacgctca taatcagcaa ttccaagatt atggcttgat gagattatgc
      541 catcaacttg gttggcttca agcatttcaa tgtattctct ctctttagca ggatcatttt
      601 gactattgca aataatcgtt ttatatccat gtttaaataa ctctatttcg agatactcaa
      661 tgagttcaga gtaaaagata ttactaatat taggaaaaat aagaccaata agctgaggtg
      721 actttccttg aagtccacgt gctaaattat ttggtttata accaagagtt ttcatagctt
      781 catgaacttt ttgttttgtt ttttctgata aatatccctt attattaatg acgcgtgaaa
      841 cggttgttgg gctaactcca gctaattcag cgacatcagt taattttgct accattttaa
      901 tattttaatt caaagtattt tccagtaggt tcgcctgatt tgatttgaat accattttgg
      961 tgattttcag ggaatacacg actagtaaac actttttctc ctttattaat aaaaatctca
     1021 atgattgagt tatctacaaa aatgttagca gttgtttctt tagtatcaat tgagcattca
     1081 cgaatcgtac caaagtcagt ggcatattga atgcctgctt tgctgcgatc taaaataatt
     1141 tttccttctt tggtatctac aatcaagcta agtccatttc ctttactatc agagaaaaga
     1201 agtaactccg atttctgatt tggaggaaaa gttagttcaa gctcataagt attatttgtt
     1261 tctgcttttg ctgtaaattg ttcagaacta gcacgtaatg atgtaatagc ttctactgga
     1321 tattggtaaa gtttaccatc ttttattgaa agttctttta caagactcat tgctccctga
     1381 taatcatact tatcagttgg ataatcgata tcaggtagtc caatccaact gacaatcaga
     1441 gcacgaccat caggagtatt gaatccttga gtcgcatacg cttcaaatcc ataatcaaga
     1501 ttttgaattt ctgaagcgcc cacaagtttt actttttcta tgtcaagcga ttgacagatc
     1561 ttaaaagtat tcggatagat attcccataa tttagctcag atttatccaa cccttgagga
     1621 caataaatga gaacaggttt tttgtctaca aatactagat ttggacactc aatcatgtat
     1681 tctgaaccag tgccgccaaa atcaaggttg ccaacttcct cccatgtttc cacgttattg
     1741 tcatccgctt tgtaaagttt gataaagcct gatttttcta aattttgtgc tccaacaatc
     1801 gcatacaatt gtccttcata attaaacatt tgtggatcac gaaaatgatc agttacatca
     1861 gcaggtttat gaataagaac attctcacct ttcgtaatgt tgtagtcttt atccatccat
     1921 gctccgattt gaagtgtatc acgttcccaa ttttcatttc gaacgtttcc tgtatagagt
     1981 aagaatagat tcccctctat ttcatatgca gaaccggaat aagcgccgtg gctatctcgt
     2041 tttgtgtctg gaagaagttc tgctcctgtt acatgaaaat gaactaaatc atctgattca
     2101 ttgtgtaccc attctttcaa cccatgagca gctccaaatg gccaattttg ataaaaaagc
     2161 gtatattttc cgttaaaaaa agaaaaacca ttaggatcgt ttaacaatcc tgttttaggt
     2221 tcgatatgat agctagcacg ccaaggtgat cgttttacat tatccttaat tttatccatc
     2281 tcatctttag tccaatcaga ataagcacga tatcgtattt ctcgaggtaa gttcatattt
     2341 tactccttca atttatcttt ttacaactag tatggtaaac gttttccgtt gaaaaatcaa
     2401 tcatttcatt taaaaaaata aactatatga caaacgtttg acatattttt aagatatgct
     2461 aaaataaatt tgcatttcag tgaaccgttt tcacgtaaaa ataaaaaagg agtgattcga
     2521 atgaattaca gtaaagtagc ttccgacgta atacaagcgg tcggaaaaga taaccttatt
     2581 gcagctgcac actgcgctac acgtcttcgt ctcgtactaa aagatgacac gaaagtgaat
     2641 caaaaagctt tagacgaaaa tccagatgtg aaaggcacat ttaaaattga tggacaatat
     2701 caagtgatta tcggtgcagg tgatgttaat ttcgtttatg acgaacttat caaaaaaact
     2761 ggactttcgg aaatttcaac agatgattta aaacaaattg ctagtaaaga taaaaaattc
     2821 aaccccatta tggcattaat taaattatta tcagatattt ttgttccaat tatacctgcc
     2881 cttgttgcag gtggtttatt aatggcatta caaaactttt taacagctgc tggacttttt
     2941 ggaccaaaat caattgaaga aatgtatcct gcgattgaag gtatttcctc aatgatccaa
     3001 ctgatgtccg ctgcaccatt catgttctta ccaattttag taggtatttc agctgctaaa
     3061 cgttttggag caaaccaatt tcttggtgct gcaattggta tgatcatgac aacaccagat
     3121 cttggtggct caactgaata ttggaatatt tttggttatc atgtttcaca aacaaactat
     3181 gcttatcaag ttattccagt tttagcagct gtttggatat tatctatatt agaaaaattc
     3241 tttcataaaa aattgccatc atctattgac tttacattca cgccattatt atcagtaatg
     3301 attactggat ttttgacttt cacagttatt ggccctgtaa tgttattgtt gtctaatgga
     3361 attacagaca ttattgtttg gctatataac actactggat ttattggaat gggtattttt
     3421 ggaggaacat attcacttat agtcatgact ggtttacatc aatcatttcc agctattgaa
     3481 actcaattac tttcagcgtg gacaaatggt atcggacatg gagactttat ctttgtagtt
     3541 gcatctatgg caaacgttgc tcaaggtgct gcaacatttg ccatttggtt cttaactaaa
     3601 aattctaaga caaaaagtct tgcttcctct gcgggtgtct cagcacttct tggaattaca
     3661 gagcctgcct tgttcggagt taacttaaaa taccgctttc cattcttttg tgctttaatt
     3721 ggttcaggaa ttgctacagc agtagcagga ttacttaaag tagttgctgt ttcacttggt
     3781 tcagcaggtt tcttgggatt tctttcaatt aatgcaacat ctattccatt ttacgtattg
     3841 tgtgaattga ttagctttgt agttgctttt gcaattactt acttctacgg taaaacaaaa
     3901 gctgctggta tctttaaagc agaagaaatt gctgaacaaa caccagttaa ttcttcaaaa
     3961 gtagcaacaa cagatccaat atctactgat gttgaatcaa cacaaacaaa agtttcatca
     4021 gaaacaattg ttagtcccat ttctggtaaa gtgattagtc tttcttcagt aaatgatcca
     4081 gtcttttcat cagaagctat gggaaaaggg attgccatta aaccaaatgg aaacacggtc
     4141 cattctccag tagatggtac tattcaactc acgtttgaaa ctggacatgc ttatggtatt
     4201 aaatcaaata atggtgctga agttctaatt catattggta ttgatactgt atcaatggga
     4261 ggaaaaggat ttacacaaaa agttactact aatcaaaaag ttagaaaagg cgatattctt
     4321 ggagcatttg accgttcaat gattgagaaa gaaggtcttg atgatacaac aatgattatt
     4381 gtgaccaata cttcggatta ttcagaagta aatatagtag ctgaaaatac agtcactgag
     4441 ggtactgatt tacttgaaat caaataaata tatttccctg agtaaagatg ga
//
LOCUS       DASJTC010000142         4440 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.146, whole genome shotgun sequence.
ACCESSION   DASJTC010000142 DASJTC010000000
VERSION     DASJTC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.146"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            113..994
                     /locus_tag="U4B19_002643"
     CDS             113..994
                     /locus_tag="U4B19_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2078307.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            1007..2617
                     /locus_tag="U4B19_002644"
     CDS             1007..2617
                     /locus_tag="U4B19_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEN2078308.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2694..3353)
                     /locus_tag="U4B19_002645"
     CDS             complement(2694..3353)
                     /locus_tag="U4B19_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2078309.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3636..4301)
                     /locus_tag="U4B19_002646"
     CDS             complement(3636..4301)
                     /locus_tag="U4B19_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEN2078310.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1203 a          858 c          873 g         1506 t
ORIGIN      
        1 aatattatgt actaaaataa taaaaaaagt tttataaaaa ggtataaaac ctttttatta
       61 aactatttaa tcttgtttag tatatatttt tagaaaaagg agtggggttt ttatggcaga
      121 aatcgtcaaa gtcacacatt tgaaaaagag ctttggtcgt ttcgaagctt taaaagatgt
      181 ctctttcacc gtacattcag gtgaagtcgt tggatttatc ggaccaaatg gttctggaaa
      241 atccacgacg attcggacac ttttagggat tataaaaaaa gacggcggaa cggcaacgat
      301 ttttgggcaa gatgtatggg aagatagttt atctatccat gaacgtcttt cttatgttcc
      361 aggcgatatc tctttgtggg gaaatttgac tggaggagcg atcatcgatc tcttcatgaa
      421 gcttcatgga aaagggagtg ctcaaaaaaa agatgaactg attcaacgtt tctctcttga
      481 tcctaagaaa aaagcaaaaa actattcaaa aggaaatcgc caaaaagtgg gcttgattgc
      541 tgctttggct gttgattcgg atctctattt atttgatgag cctacttctg gtttagatcc
      601 tttgatggaa acgattttcc aagaagaagt tgaaaaattg aaagaacagg gcaaatccat
      661 cctactttct tctcatattc tcagtgaagt cgagcgacta gcggataaag tcgtgatcat
      721 acgcgaagga acactcgatg aagaaggaac actcgatgaa ttgcgtcatt tgactcgttc
      781 gaccatcact atagaaacag aagcagagaa tagtcactta gcacaattga acggtgtcca
      841 tgactttgtg caaaaaggtc acctcgctca atttgctgct gataacgaag ctttcgatgt
      901 gattttagct gaagcgtcca aattaggtgt gaaaaaattt gaatctattc caccgactct
      961 tgaggattta ttcatgagac attatgaagg atgaggagga agcgaaatga ctgataaatt
     1021 ttcacgctgg tccacccttt tttctcaaaa ccttagccgt gactggaaaa agatcctgat
     1081 atggctagtc ggtgtcactg ctttttctgg tggttttgtc cctgcgtttg aagaattgac
     1141 ttccggacaa ggattagctg gattatacga aaccttgcaa aacccggcaa tgattgcaat
     1201 ggtcggacca actccagctt cgaatgctgc tgagtatact ttaggagcga tgtatgcaaa
     1261 tgaaatgttg cttttttgca gtttgtttgc tgctgtgatc tctgttttac acgtggtgag
     1321 ccaaacgaga aaaaaagaag aacttggtct gattgaactt attcgttcct ttcgagtcgg
     1381 acgtcaagca aactctttag ctgtcatcgc tgaaagtatt ctgatcaatt tgcttttagc
     1441 aatatcgatc agctgtacga tgattttttt ccaagcggat tcgatcactg cagaaggtgc
     1501 ttggcttttt gggctatctg taggtagtat ggggattttg gggagtgccg ttgcactgtt
     1561 tgctgcacaa ctgatgccaa gttcagccag ttcgaatggt ctgtctttag cgtttttagg
     1621 tggactttac cttctacgag caataacaga tgtagctgat tcctcccttt cgatgtggaa
     1681 tcctttaggc tggtcttatt tgacctatcc atttacaact aacaactgga ttcctttgct
     1741 ttatttgttt ttgttcagtc taatcttgat aatcagttca tttgttttag aggaacatcg
     1801 agatttgaac accggctata tccccgaacg tgaaggaaaa ccgtatgcta aacgttctct
     1861 tttatccttg cgtggtttat tattctatct aaataaaggg atgacaatca gttggcttat
     1921 cacttttggt ttgatggggg cggcatacgg ctctatctac gggaatatgc aacagttttt
     1981 agaaagtaac gacttaatga aacagatgtt tatccaatct ggcgtctcaa ttgaaaaaaa
     2041 ctttacaagc acgatcatgg tagtcttgat ttgtctagct gctattttac caattgctag
     2101 catcaatcgt ttatttacag aagaaaccca cggacgtatg aatcagctgt tcgcaacaaa
     2161 aatttctagg aaccaacttt attggaccaa tatgttacta gctattctat ttgctatatt
     2221 ggcaatcctg attagttctg gtgcactagg gctttctgct ttggcttcga tgaaggatgc
     2281 gaaagacatg gatctattga tgtttcttaa agccgggttc aatttcctgc ctgccgtttt
     2341 agtgtttatc ggaattagtg gctttctatt cggatggctt cctcgaatcg gtaaacttgc
     2401 ttatatctac ttagcttact cctttgcact caattacttt ggcggaattt tggatttacc
     2461 agaatggttt tccaaaacag ctgtccctag ctgggttcca cggatgccaa acgattcttt
     2521 tgatctttcc gtgttcctta cgttatcatg tatcagcctt gcactgttcg tccttggttc
     2581 tattggttat aggcgaagag acttactaga aggataataa aaaagcatgt acaatttcat
     2641 gctagcctcc aaatctgggt caaaaatcca acatttggag gtcatttttt tatttacaag
     2701 tcttctaact gctctttatc aatgatccag acttctcgat tctttctttt tacataacca
     2761 ttctcctcta ataatttaaa ttttctagat aaagtttcgg gggtcgtacc gataaatgca
     2821 gcaagttctt tcattttcat tggcaggaaa aagtgatcct tccctgtcgc tttagctaag
     2881 tcaagcagat agagagcgac tcgttcttct actttttcca tcatcaaaaa agttgtctgc
     2941 tgctgtactt ttgttaattt ctcaacgttg attgctaaaa gtttcagact aagggcggga
     3001 taatgcagta agagttccga gaaatctttt tgtctcaata tgcaaatttc cgttttttct
     3061 agtgcttcgc caaataaaga ctcatttgga acgcctagca actggctctc tccctcatat
     3121 ccgcccggct ctattactcg taaaagctgt tcctttccgc tagcagataa ttgatacact
     3181 ttcatactgc cggaggccac gatcactaat tgaggttctc cattcggcgc aagaatctgt
     3241 tctccttttt ccacatagtg atgtttgaca agtgcgtgta ctttttcttg atcttgctgt
     3301 actaagtgat tgaacaaagg aacaaatgaa acacataaat gatcatgact cataatctct
     3361 cctccttcta aacagctatt tctaataaag aaacataact tcccttattc caactgataa
     3421 ttttctttta tcatagcata aacgtcaagc gaaccagata ccagcaaata gagtggattt
     3481 ctttttgtcc cttgctttat tttccttctt ttgagacctt aagtaattga ttcctatcaa
     3541 ttttttaaat atttactttt tgaataatca aaaacaaacc ttcactcgca agccgagagt
     3601 aaggtttgtt tttcgcattg cttatttcgt gttgtctatt gaaatttttc cgcacctagc
     3661 ttttgatata gtggtgctag ttcttgtaag gtttcagcgg ttatttgctc aacgtgttcg
     3721 atttcttgcg gatagaagaa tagtttaccc atttgaaagg ttcggtctct catcgctttt
     3781 ttcaactctt cttgtttctt tgtatcggac aaatagtcag acaaagctaa gtggtcgtca
     3841 agttcatgtt ctggttgcgg ccatatgtaa taatccttga tatcgactgt tttgctccac
     3901 tctttcagta tcgcaagcaa ctggttatac gctgcaatgc taccatatcg ttcttcgctt
     3961 ttataatgtt ggtacatcaa atagatttgg tattgttttt gattcaacaa aactgctaga
     4021 caggcattct cgtcttgacg agattcgctt cggtacgcgc tccaaaaatg actgcgtaaa
     4081 ttccagccat tcgtccagct ttcgatttta ggtttgctca tgccgtacat atcaggcaaa
     4141 agtgcagctg tctggagctg aatctctttc catttttgcc agtgttcttt atattctttc
     4201 ttgatttgtt caatcttatc tgttggatgg ctctctttga gctgtttgaa tgcgtagatt
     4261 ggtcgatcaa acaactgata atctgatttt ccaagttcca ttgtttcgcc tctttccgtt
     4321 atcttttgta ttctacctga attttgcgat atggacaaat agaggttgtg aaaaagctgt
     4381 tctgcatcaa gtcataagaa cagccaaaca aaaaactgcg atgagcatca ccgcagtttt
//
LOCUS       DASJTC010000143         4434 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.147, whole genome shotgun sequence.
ACCESSION   DASJTC010000143 DASJTC010000000
VERSION     DASJTC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.147"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(543..2048)
                     /locus_tag="U4B19_002647"
     CDS             complement(543..2048)
                     /locus_tag="U4B19_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEN2078311.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="U4B19_002648"
     CDS             complement(2045..2731)
                     /locus_tag="U4B19_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2078312.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U4B19_002649"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U4B19_002649"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEN2078313.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          730 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa agggatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DASJTC010000144         4402 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.148, whole genome shotgun sequence.
ACCESSION   DASJTC010000144 DASJTC010000000
VERSION     DASJTC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.148"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..361)
                     /locus_tag="U4B19_002650"
     CDS             complement(<1..361)
                     /locus_tag="U4B19_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078314.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYT"
     gene            complement(569..1495)
                     /gene="glsA"
                     /locus_tag="U4B19_002651"
     CDS             complement(569..1495)
                     /gene="glsA"
                     /locus_tag="U4B19_002651"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEN2078315.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(1518..2945)
                     /locus_tag="U4B19_002652"
     CDS             complement(1518..2945)
                     /locus_tag="U4B19_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2078316.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(2976..4190)
                     /locus_tag="U4B19_002653"
     CDS             complement(2976..4190)
                     /locus_tag="U4B19_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEN2078317.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         1383 a          931 c          778 g         1310 t
ORIGIN      
        1 ttgtatatac tttgctcaaa gaaataaatt caagatcagc cagttctgta ctcgtgaata
       61 taccattaaa ataataactt aaaaacttta aaaaataaga ttgaccgtac agaaactgtg
      121 tgagctcaga aagagcaata ccatgtttga atacgagttt atacttatgc ttctctctta
      181 cttgttcttt tatacaatat tctagacact cggctagact ttctagatcg tgaaggatcg
      241 ttggtgtagt cacccttaga gaaatagctg tttgttcgat gtctatttct tggaattcaa
      301 ataattgttc acacagatac acttttctaa atatatttcg ctcgatgtaa ttttccagca
      361 ttcctgatac acgcctccag tttttcttat gattttattt aatcatatct gtaaatcgta
      421 tttttgtttc ctttttattt agaaaagccc tttcaaaaac caataaaaaa agaaactggg
      481 acaaaagtca aacaagctca caatttcagc ctcattgtag gtagctttga gccgtttgga
      541 cttttacccc agcctctttc aagaatggtt attcaaagat gtcagcgtct agttctttga
      601 ctacatcttt caacaattgg ataccagctg cgctgttacc aaatggatct agtgccgggc
      661 tgaagacacc tatgccgtaa cgatttggca cagctgccat caacccgccg cctacaccgc
      721 tttttgcagg tacaccaaca tggacggaaa actctcctga ttcatcatac aaaccagcag
      781 ttgtcataat tgatttgacg attgtagcac tttcttcagt aatgatttgt ttgccattcc
      841 aaggagcaat tcctttattt gccagcacag cagctaattt tgctatgcct gtagcggtga
      901 cattgaggga gcattgtctg aaataagcat cgagtatctc cggtacgtct ccttcgatca
      961 tttgattacc tttcatatag taagcaagag agcgattgat atcacctgtt cttgattcag
     1021 aatgatagat ttcttcattc aatgtgatat taggatcatc acagatctct ttcataaatg
     1081 acaagattcg ctcaaatttt tcatctgcat ctttaccggc aatcaacgag gttgttgcaa
     1141 tcgctcctgc attaacaaaa ggattcattg gatgtttgcg atgattgatt tccatattca
     1201 tgatagaatt gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag actttttcta
     1261 ttccgttgtc tttgattgct aataacaata cgggaacttt tgacatgctt tcgatggcaa
     1321 agcgcacatc ggcgtctcct ccctcaatca gttgttttgt atccaaatca taaatcgcca
     1381 tacctaattg tttgggatcc acttcagcta atgctggaat gtaatccgcc acttgtcctg
     1441 tttgataata gtggaaattt tttccaatca attgttctaa ttcttttact tccatttgaa
     1501 taaccagctt tcatttttta tttttctgca tgctggaatt tatgacggca tgcgtagatc
     1561 gtaaatggaa taactaagac gataagataa gaaacaatca gaatcgtttc ataaatccca
     1621 tgctgtccag ctgcgatact agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa
     1681 gtcaaaagac ctaaacaagc aacgattact tttacaactt ttccgcctgg aacctgatag
     1741 gctctttcaa gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa aaacataaag
     1801 tacaacatca ggtagattac aacagtcaat gacatcgctg caagaaatga aagattattc
     1861 cccccgccgc ttaatgtaaa gacggctgcc cagattgttg caacgatccc atttgcgatc
     1921 atcaaatgag caggaacacc gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct
     1981 tcttctgctg ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc aatttctccg
     2041 atgacaccta aacaaacaag aatcgcaaga attttcgcaa tccatgcaag atttggatca
     2101 atgtgtctta ataggaattc taacgattga atcacccctg tattaagaga caaaccatgt
     2161 tgaggtactg tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata
     2221 accaatacaa acattgctaa tggataatcc tttttagggt tttccatttc atttgcatga
     2281 ctggcagatg tttctacgcc catatatgct aaaataaagg aaacaaaaac aaccagcgta
     2341 ggcaatttcg aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg
     2401 cctgaagcga cataatggat ccctaagcca aacaagacta atgctggcaa caagatacca
     2461 gctacaaaac cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt
     2521 aaaatagccc agaaaataat caataggatt gccaatttca gatatttatt gttttgtata
     2581 gcaccaatac ccgttgcata ggataatgca ccagttaaga aatacagcat ggtcaagtag
     2641 ccaactgtta tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat
     2701 gtgcgactga cccaagtata gacaccgcct ttttcccatc ctttcaccgt tgccatctct
     2761 gctgcgcaca atgccacagg gataaaccat agcaaaccgc ctaataacaa gaaaaatact
     2821 aagctgaagc ctgaggtcgc gaatgtggga tattcatata atgaaacgac cattgagatg
     2881 gtcatagcca aaaagccaaa gaacgttaat ttttttgcag gaattttcgt tttgctttgt
     2941 tgcatgaatc ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag
     3001 aaaaagtcca atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc
     3061 gccgatttta aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca
     3121 atcacttgaa tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat
     3181 gcaaagatcc ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca
     3241 tcaagtgcat catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata
     3301 cccgcaatga tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta
     3361 atggctgcca atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct
     3421 tttacttttg caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa
     3481 ccaaatgcat tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac
     3541 cagccaaacc aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt
     3601 ctttctccta aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat
     3661 ccagctgtcg tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa
     3721 aatccgccac cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata
     3781 acaagtataa tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga
     3841 acggtaatca cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca
     3901 ccatgaaaca tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt
     3961 ccaataacac cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata
     4021 atcgttgtta tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc
     4081 cgtgctaatc cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta
     4141 cataaaatca tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc
     4201 tcctttctac gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg
     4261 aattaatact tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct
     4321 ttttcgtttt tcacacaaat aaagacaaaa aataaacggt cttcttttta tagaaaaccg
     4381 tttattttcg tcttttttta tt
//
LOCUS       DASJTC010000145         4335 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.149, whole genome shotgun sequence.
ACCESSION   DASJTC010000145 DASJTC010000000
VERSION     DASJTC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.149"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            127..579
                     /locus_tag="U4B19_002654"
     CDS             127..579
                     /locus_tag="U4B19_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078318.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="U4B19_002655"
     CDS             593..1282
                     /locus_tag="U4B19_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078319.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="U4B19_002656"
     CDS             1407..3758
                     /locus_tag="U4B19_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEN2078320.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="U4B19_002657"
     CDS             3838..4233
                     /locus_tag="U4B19_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078321.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1526 a          548 c          867 g         1394 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatt
//
LOCUS       DASJTC010000146         4200 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.150, whole genome shotgun sequence.
ACCESSION   DASJTC010000146 DASJTC010000000
VERSION     DASJTC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.150"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..705
                     /locus_tag="U4B19_002658"
     CDS             4..705
                     /locus_tag="U4B19_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEN2078322.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1277..2293)
                     /gene="tsaD"
                     /locus_tag="U4B19_002659"
     CDS             complement(1277..2293)
                     /gene="tsaD"
                     /locus_tag="U4B19_002659"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEN2078323.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2290..2754)
                     /gene="rimI"
                     /locus_tag="U4B19_002660"
     CDS             complement(2290..2754)
                     /gene="rimI"
                     /locus_tag="U4B19_002660"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN2078324.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2761..3303)
                     /gene="rimI"
                     /locus_tag="U4B19_002661"
     CDS             complement(2761..3303)
                     /gene="rimI"
                     /locus_tag="U4B19_002661"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEN2078325.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3287..4009)
                     /gene="tsaB"
                     /locus_tag="U4B19_002662"
     CDS             complement(3287..4009)
                     /gene="tsaB"
                     /locus_tag="U4B19_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEN2078326.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1229 a          908 c          726 g         1337 t
ORIGIN      
        1 ttaatgcgtg ttaataaaat cgattttcac ttttcaaaac tcagatatca acctgatttt
       61 gaacatttct cggatgaaga gttccaacag atcaaggaac atactgttct tcgttcttat
      121 aaaaaaggac agatcctttt cgacgatggt gatcccagac aacggttgta tatcttgacc
      181 aaaggactcg ttcgtttaga acgttatgat gaaagtgcaa cttattacta ctatgactat
      241 gtgaatacaa ataacctttt tcctatgtca ggaatgtttg gagaagagac ctacacttat
      301 acagctcaag cgatgactga tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa
      361 ttagcacgaa aaaatcccga acagttgatc tatatgatac aaaaaatttc taaagtagtt
      421 gaaatacatg aattgcgtat ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa
      481 caaaatttat tcattttgaa agaagaattg ggcgtaccaa caaaagatgg tcatatcacc
      541 attccttatc cgatcacctt aaaagaatta gcagtgaata gcggcacgac acgtgaaact
      601 gctggacaag taatcaaaca actacgtgat tccggactgt tagattatga caaaaagcgt
      661 tttactttct gtaagacagc tactactcca gctatgagag aataacgcga acaaaaataa
      721 gcagtgaccc tgatatttat cagggtcact gcttattttc ctccctaaaa cttctgaaag
      781 atccggcagt tttagatcac tgggcaagac tcaaagattt tctatgtgtg tttcagttat
      841 gtaatattca ctgtttgcaa aaaatactga tagattaact agaggaagcg tcttgtcctc
      901 atccgaccta gattattcga tattcctcga caataaacga ttaatcagtg agggcaatca
      961 gaggaacgga taatgatgaa taagctgtaa aaaatagggc tttgacaaaa gcgtgtcaca
     1021 gcccttattt cgattaaacg gtgtgtacct gtcaactctt cggtcacaag tcataatgtt
     1081 cataaacatg aggagctgtt ttttgaaatt gttccttgtg aatcagagtt aaacgacagg
     1141 cttacgaact cttgattaaa cggcgttcaa aagcttgcat acgcggtaat aagaacagcc
     1201 aggtcaaaaa catgagaagc cgtttttcct gggctgttct tattacttga tgcagggcag
     1261 atttttcaca acctctttat tcagataaaa ccaagcttgg atcagcattc aactgatagc
     1321 tagcaaaatt tcttttttgc atttccacat gtgctgctgc tccaatcatt gcagcattat
     1381 ctccacaaag tcgaagaggg ggaatcacta gttctacctc agggagttta gttgccaaag
     1441 catcctgcaa tccttcacgc aacccttgat tggccgctac acctccagct acaatcagtt
     1501 gcttgactgg ataattttga caagctcgaa gtgttttagt aatcaataca tcgatcacac
     1561 ttgcttgaaa gctagctgct aaatcatttt tgtctagatc ttctccacgc tgttgtgcat
     1621 tatgcaccaa attgataaat gcacttttta atccgctaaa actgaaatca taattatctt
     1681 catggatcat cgctcgtgga aaatggtaat tatcttttcc ctgatgagct agctgatcga
     1741 tttcttttcc gcttgggtaa gaaagaccta atactcttcc aactttgtca taagcttctc
     1801 ctgctgcgtc atctcttgtc tccccaatga tttcatatga tccatcttct tgcatataga
     1861 ctaattctgt atgtcctccg ctaacaagaa gtgccatcaa tggaaactgg aatggtttaa
     1921 ccagacgggc tgcatagata tgccctgcca tatggtttac aggaataagc ggcaattggt
     1981 gtgcccaagc aaaagcctta gctgcagaaa taccaatcag caacgagccg acaagtcctg
     2041 gtccataagt gacagcgact gccgataaat cttctgccga cacaccagct tctaccagtg
     2101 catcttccaa gcataaggtg atttgttcta cgtgatggcg gctagctact tcaggaacta
     2161 cgccaccaaa acgtttatga ctgtttattt gagaagcaac gatgttagat aagatctctg
     2221 ttccatttct aacgaccgct acactcgttt catcacaact tgattcaatc gccagaatca
     2281 attcttcatt catttcatcg tctccgtttt taactttctg ctcataatga tcgcatcttc
     2341 cgtcggattg cgataatatg atttccgttt acctaatata cgaaactttt ctgattcata
     2401 aagttttctt gctgcttcat tagatagacg tacttctaaa aaaacatgca gcgaatcttt
     2461 tatttgttcc gtaatcagta attgtaacaa atgccgtgca tgcccttttc cttgttctga
     2521 aactgcaaca gcaatatttg tgatctcgac ttcatctatt actttcgagt atccaataaa
     2581 gccacagatc tggttatctt caactaaaag cagataatca gagtgaggct gttggatgtc
     2641 tgccaaaaac tgcattttcg tccaaggtga accatgtgga taagcatttt cgctaatcat
     2701 ccatacgcat tctgccaagt acgcttcatc aaaatctttt tttctgcaaa tcaactaatc
     2761 tcactcattt tctaaaagtt ttatcatatc caacgcatct tcttttgttt cagtgtaata
     2821 tctttttttg atcgttgttg cttgaaaacc taacttccga taaagtcttt gtgcatcttg
     2881 attactcata cgcacttcta atgacatcgt ctcacagcga ttcattatag caaagttctc
     2941 gatttcgtca ataagcagcg aaccgattcg tttacgctga tatgccggtc gaacggcaat
     3001 attcgtgata tggcaatcta aaccgaacgc tcttgaaccg ataaaaccaa tcattcttcc
     3061 attatcaaag atacctagat atagatgagg aagcggtgac ttcaattctg atataaaggc
     3121 actttttgtc catggcaatt cgccattata gacttctcgt tcgacatcca gcagctcttt
     3181 gatatcttct gtagtcaatt ctcttagtac gtaacgacga tcgatttgtt cgattgtttt
     3241 ttctgtataa atcattttat cttgaaaaaa gcttctggca tacgctctaa attttttcaa
     3301 cataactttc gtctccaggt gtgtgcgtct ccaaccattt ttcttctgct tctacccgtt
     3361 ttaaatagcg aggaagaaaa gcttgtttct ctgcaactgg ttcagctaaa gcacctaatt
     3421 gagccacagt tacaccactt ggaatatccc atgctggaac tgtattaatc atcgcactag
     3481 gcaatatttc ctttatttca tagaagaatt tgtgacaatc actcccgacg aaataaatag
     3541 cttcattcag cttttgtagt tgtgtgaaca aatcagaagc agaaatatgt acatcttcaa
     3601 gtacaatttc taatctattt tctttccagc ggtatgcccc agcgtataca ttttttcttc
     3661 gagcatcaaa catgggcaca ataattttat ccacaccgat gcaattagcc gcgatggttt
     3721 tcagactaga tactcctact agctctttat ctaatgtata agcaatcgtt tttgctgtcg
     3781 ttacacctat acgaagccct gtataagaac cgggaccatc agaaaccgcg attcggtcaa
     3841 tgtcttttgg acttatttga agatctgcaa acaattggtc gattgcaggc atcagtgtta
     3901 cactgtggtt gcgtttgata tttgtttgta tttgtccaag aatttgttcg tcttctacca
     3961 caccgatcgc caaagtttgg tttgcagtat ctattcctaa tgtaatcata tatttgatcc
     4021 tttctgctat gacattctcg ccttccattg tagcatactt ttttattctg aggatgaaaa
     4081 taccaaaggc aaaactagta caatgataca tgattatctg ctggcgggca aattttgccc
     4141 cttcctgaaa cacaaaaaag ctgcggtatc acttcatttc cccacagctt ttccatcttt
//
LOCUS       DASJTC010000147         4067 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.151, whole genome shotgun sequence.
ACCESSION   DASJTC010000147 DASJTC010000000
VERSION     DASJTC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.151"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..1155)
                     /locus_tag="U4B19_002663"
     CDS             complement(<1..1155)
                     /locus_tag="U4B19_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2078327.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
     gene            complement(1158..3407)
                     /locus_tag="U4B19_002664"
     CDS             complement(1158..3407)
                     /locus_tag="U4B19_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEN2078328.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3504..3959)
                     /locus_tag="U4B19_002665"
                     /pseudo
     CDS             complement(3504..3959)
                     /locus_tag="U4B19_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
BASE COUNT         1311 a          743 c          580 g         1433 t
ORIGIN      
        1 tctagtattt tttttatttg tcgcaaaaaa tgttacaaat aggatcacaa taaatattcc
       61 tgcgaataca aaaaatggag cagtcaaatt tgtacttctt gttagaaatt gtgctaattg
      121 catagcaata ggagctataa aattaccaat attacagcca ataaaaatca atgaagtagc
      181 aaaattaatt gtattctttg tagtgacttt atctaagtta ctaaagataa atgggaagca
      241 ccatgatcct gttatacttg ataaaaataa acccgttagc aggaacgtca tattaccgtt
      301 tgacacagca attaacaaat ttgaaattat aaagaatata attcccaagt atagggttcc
      361 ttttcctatc cacttattta ttggtccaaa cataaatcct gctagtattc ctaagatagg
      421 cattagagct aaatagttac tagcattaaa gtttacccct ttaatttcag ttgcaattga
      481 tgcaaatcgt acagaaatag caatactatt cattacgagt aagattgcaa atagaactag
      541 ggcaaaaacc attggattca tcttttcttt tacatgattt tcaggtatgt cactattttc
      601 atctctaatt tcaggaactt ttagcgcgaa taagagagct acaggaaacg caattgcata
      661 gactaaaaac gaagccgtcc aattaatgtt cactaaaact ccagcaagaa aaataaggat
      721 tgtctgacct aaagcttcca ttgaatttct aatacctaat agcgttgctc tagtattgcc
      781 agagtataaa gagctgatat atgtcacagc caatgaatta tatactccta aaccggcacc
      841 taatattact cgactaataa gaacggtttg gaaggtagct gtaaatactg gaataattcc
      901 tcctatacca gctaataata aacctaaaat tattgtcttt ttcattccta ttcttttggc
      961 aaagtaacta gataataaga taaacaaaat aacagttata cttggtgcag ttcctaaaag
     1021 ttcactttga ctttgtgata tccccaaaga ctgtttcatt tgtggaataa caccattaat
     1081 tgcttgagag cttgttaaca ttagtgaaat acttaagatt gctccttttt gaagtaagct
     1141 tttttggtta ttcatctcta cttttcctcc tgtgttataa cccaaatctt ggaatcaaaa
     1201 tcaaatgttt ctgggcgtcg attctgatca tttatttgtc cactagatgc atctgaacag
     1261 actagtcctg ctctcatcaa ttccattcca gatcgcaaac tattttgtcc tacattataa
     1321 agtccgtcat tatctaaccc ttttaatttc agtcggcggt aaggagcatt tacctcattt
     1381 aatgttttat aatgagcaac aatagccgtt ttcttatcat gactaacaac catccacgcg
     1441 gtttgattat catcatcgaa tggtgataat agtctataaa aagtaccatt atgaattatg
     1501 gttctatatt ttttataaaa ggcaatttgt tcttttacaa tcctcaaatc gccttcactt
     1561 aattcattta agtcaagctc atatccaaat gcaccaaaaa aagctacatt tcctctagtt
     1621 tttataggtg ttatacggtt tgtttgatga tttggaacaa ttgatacatg agcaccaata
     1681 gacgaaagag gatacagcat actagttcca tattgaattt ttaacctttc aattgcatct
     1741 gaatcatcac tagcccaagc ttgaggtgca tagtgaagca ttccaggatc aaagcgccca
     1801 ccacctgatg aacaagactc aaatagtatg ttaggatatt ttgttataag tttttcatat
     1861 aagcgatata cgcctagtac atatctgtgg aatacttctc cttgttgatc tgctgacaag
     1921 tgactgtcaa atacatcagt caatggacga ttcatatccc acttaatgta atctaagttt
     1981 gattcatcaa tgattttaca catttgatcg aaaatatttt caacaacctc atctaaccca
     2041 aaatttaata catattggtt cctaccatga ttaggttttc gattaggtgt agaaataatc
     2101 caattcggat gtgtgcgata taactcacta tccttattca ccatttcagg ttcaaaccat
     2161 aaaccaaatt tcataccaaa actacgaatt ttttctgaaa ggctgccgat tcctctaggt
     2221 agtcttttag gatttacatg ccaatctcct aatcctgcac aatcgttttc tctttctcca
     2281 aaccagccat catctaatac aaataattca ataccaacat caaatgcttt tttagcaata
     2341 tttaccaact tttcttcgtc aaaatcaaaa tatgttgctt cccaattatt aattaagaca
     2401 ggacggtctt tttctcgcca cttaccacga gctaagcgtt ttctaaatag ttgatgaaaa
     2461 gtttgactca tggcatttaa acccttactt gaataaacca gaatcgcttc tggtgctgta
     2521 aatatttctc ctggattgag tttccaatta aatccaaatg gattgatgcc aacttgtagt
     2581 ctagtaacat cccatgtatc tacttctgct tgaattaaga agtttcctga gtaaactagt
     2641 gctgcaccaa tagcttctcc ttgattttca tcagtatttt ttctttttaa agtaacaaat
     2701 gggttatgct gatggcttga aatacctcga gtagattcga ttgattgaat tccttgttgt
     2761 aaagtacgcg acttaatagt tctttctcta ccccaagcac cagataattg ttgccattca
     2821 taatttgcat cgggtaaatc caatgataaa ctgctaatat tctcgattat ttgaatatct
     2881 tttcctttat tcataactcg cgcgctacga gtaataacag gataatctct ataaattgta
     2941 tataataatt ctacttgaac atttgtaact gaatctaaaa tataaacttt caatgttttg
     3001 ctttcatcat tattatcaac ataggtagca ggtaaacctt ctagtttagg ttttccttct
     3061 tccacttcgt acccttgata gacaaaatct gataagcgag agccattttt ctgtagtaat
     3121 gaaatagcag gatgtctaaa atctgttgta ccatattcag gaaattcttg ttttaagtgc
     3181 tctaacgaat atagcaaatc gttttcattt ctataggttg tcataggccg atgatgcatc
     3241 tcaattagat gactatagtc attatctggt aagttctcac cataatataa atgcaataat
     3301 ttaccatctt ccgatatctt aaaaatgtaa cttatctcct gattaaacaa atgaaattgt
     3361 tggttttctg atcgataaat aatattcatt tcattatcct tatacataag ctctcctccg
     3421 tttctttatt gcattataaa aaacgctcac ataaatagta agcgtttaaa gtaacgaata
     3481 tttatcaaaa tcaaatgttt agtttattca ccattaatga ttcctagacc taatctacgt
     3541 tttcgatata ctcccggtga aacaccattt gctttcttaa aggctttaga aaaagattgt
     3601 actgttgaga atcctatctt gtcactgatt tgctggattg aatattcagt tgatgataac
     3661 agatcgtttg caatagaaat tttgatttca ctaatatact cttttattga tattcccata
     3721 tcattcttaa acaatcgtga taaataattt tcgtgtatcg atagactgac agcaatatct
     3781 ttaacagata atttttgata cgcattctca cgtattattt caattgtttg acgaacaaaa
     3841 ggattcatct ttttagtaat tatcccttcg ttcagataac tagctgaatt taagaggagt
     3901 tctaaaaatt tataaacttg tttttgtctg gacaattcac tgactagatt gtcctgatct
     3961 ttcagctgag tgttaggttc tgttactaaa tatatagaat tttccatatc caccctccta
     4021 tgttagattt ggataatttt tgttttatta tgtttatatt attacat
//
LOCUS       DASJTC010000148         4066 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.152, whole genome shotgun sequence.
ACCESSION   DASJTC010000148 DASJTC010000000
VERSION     DASJTC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.152"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            85..1434
                     /locus_tag="U4B19_002666"
     CDS             85..1434
                     /locus_tag="U4B19_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2078329.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="U4B19_002667"
     CDS             complement(1562..2152)
                     /locus_tag="U4B19_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEN2078330.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="U4B19_002668"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="U4B19_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEN2078331.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DASJTC010000149         4040 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.153, whole genome shotgun sequence.
ACCESSION   DASJTC010000149 DASJTC010000000
VERSION     DASJTC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.153"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(1014..1304)
                     /locus_tag="U4B19_002669"
     CDS             complement(1014..1304)
                     /locus_tag="U4B19_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078332.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="U4B19_002670"
     CDS             1706..1942
                     /locus_tag="U4B19_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078333.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="U4B19_002671"
     CDS             1949..3901
                     /locus_tag="U4B19_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN2078334.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT         1403 a          685 c          700 g         1252 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta
//
LOCUS       DASJTC010000150         3931 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.155, whole genome shotgun sequence.
ACCESSION   DASJTC010000150 DASJTC010000000
VERSION     DASJTC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.155"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..527)
                     /locus_tag="U4B19_002672"
     CDS             complement(<1..527)
                     /locus_tag="U4B19_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078335.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTT"
     gene            complement(589..1269)
                     /locus_tag="U4B19_002673"
                     /pseudo
     CDS             complement(589..1269)
                     /locus_tag="U4B19_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1591..1812)
                     /locus_tag="U4B19_002674"
     CDS             complement(1591..1812)
                     /locus_tag="U4B19_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078336.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(1910..2155)
                     /locus_tag="U4B19_002675"
     CDS             complement(1910..2155)
                     /locus_tag="U4B19_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078337.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            2395..3009
                     /locus_tag="U4B19_002676"
                     /pseudo
     CDS             2395..3009
                     /locus_tag="U4B19_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(3379..>3931)
                     /locus_tag="U4B19_002677"
     CDS             complement(3379..>3931)
                     /locus_tag="U4B19_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEN2078338.1"
                     /translation="NIRRFLTKKVNYLIEKAQNNLESKRFDLDSISYKTPSVINKKVT
                     GTDKDFDDNLDIFDPIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT         1252 a          716 c          570 g         1393 t
ORIGIN      
        1 atcgtagttg gatacgtaga tgaaacctca actatagttc ttttccccgt acctttaata
       61 gacactaaac cattgtcatt ttctatagtg ctctttttcc catttacttt cacaactgtt
      121 ccccaataat tgaatacagg taatgtgata ttttgctcat cggcatcttc tactgtatag
      181 actattttgg aattatatgc tttatgagat gcattctccc accgatcacc aacttttact
      241 tcatgtttct ttaaatgatc tttatatttc aatgcatcta atggtgcata gtcatatagg
      301 cctccagttc ttgaggatgt ataacgtgta acatcttttt tggtcataac taaacgacct
      361 ttatacgact gaattttatg cgaataattt gcttctgttg ctgcagtcac agtaaaaata
      421 ataagaacta ttcctacaag gctccacata ctttttttgt tgcttgtttt ccactttaaa
      481 aacactatgg ttaagattaa tgaaccaaaa agcacttgaa aacccaaggt tctcggttct
      541 gttgcgaagt ttgaaaatca aacgcgccct ctctgaactc caaatatctt aggctgttat
      601 tcccattaat accttgattt cagtagacac cgaaaagccg aagagcgttc catttcttcg
      661 gttcttttta tatattcctc gaatggtctc catgccctta atcgtggaag aggctgtacg
      721 gagactttga taaaatttat ttcgtcgttt aataggtcga tggtcttgtt ctattaaatt
      781 gttaagatac ttcacagttc ggtgctctgt cttagtatat aaacccacac tctgtaactt
      841 tctaaaggcg gagccaagag aaggtgcttt atcggtcaca attgctttcg gctcaccaaa
      901 ctgtttatgg agtcgtttta agaaagcata ggctgcttgc gtatcccgtt tctttcgtaa
      961 ccagatatct aaggttaagc cgtccgcatc aattgcacga taaagataat gccaacgtcc
     1021 cttaattttg atataggttt cgtccatttt ccatgaatag aaggattgtc tatttttctt
     1081 cttccaaaga taatagagga ctttgctgta ctcttatacc caacgataaa tcgtagtatg
     1141 acaaacattt attccacgat catataacaa ttcctgaact tcacgatagc ttagattgta
     1201 acgcaggtag taaccaacag cgacaataat gacgtctttt ttgaattgtt tgcctttaaa
     1261 atgattcatt actctgtcct ctctgtcttt tttctcaatt ttacactaaa atagattttt
     1321 tggaaaactt tgcaacagaa ccgtttttac tatatacttc ttcccagaac ttgtccgtct
     1381 ccttcaaatt atctctacct ctcgagttca ttgggtggtt tgcccattta ttaaacatgg
     1441 aatcaccttt ctcatactca tatatttatg tcctatttca attatattta tatatgctta
     1501 aataaatttg tatcctgaaa tttattgtaa tatgaagctg tattttacat atagtaacca
     1561 aagagttaat aaaataaaaa gagcgaacca ttatagatca gctctttttt cattgctgtt
     1621 tttaatctta tctagaatca cttcaaatgt ttcaaaggta tttacatcta actgacataa
     1681 ccaaagcaag tacttctttt ctttgtaagt ccaatcaata ttacggtaag aggacattag
     1741 attatggtac cttgctcttt gttcaactga agccaattcc cagcgttttt caaatatttt
     1801 ttcttcttcc attattgatc cctcgcaaga cgctacctca aacttaaata atgggcagcg
     1861 aaagtctaat tccttagatc taagtatagc aaacaaatat gaaaaaaact tagaaggtag
     1921 taaaagttct tccgtctttt gctttgcttc tattcttaat gtgtttcgtt atcggtgccc
     1981 tacaaagatt ttcattaatt acacactcat ttattgttgt gtattttctg acatttctct
     2041 cttgatcaat tattttaatt ccatgggcat cctgatagtt cataggagag gagcggctat
     2101 cagaaatagt tgcacctatc tcttgaactt cttttacaac ttctggaaaa tccatccatc
     2161 ctgcttcagc tgtcaaaagt aataatctat aatatttatc tttccttttc aatctaagaa
     2221 atcgtcaccc cattcttttt cttctattat aaactattta acttttagaa ttcaaaaacc
     2281 gtttgtctat ttagagttta ccctaaatag acaaaaatat aacattgaaa aatttagagt
     2341 tctaacgtat gattagggtg tattataaac gttatagaaa agtagggaat aaatttgaaa
     2401 attgcttata ttagagtttc atccatcgat caaaatgaac aacgtcaaat tgaagaaatg
     2461 aaaaagtttg gcgctgaaaa aatttttatc gaaaaacaat ctggagcgac gattagtcat
     2521 cggccgattt tccaggaagc tttagatttt gtcagagagc aagatatttt tattgtagaa
     2581 gccattgatc gacttggacg caattatgac gagattatcg attcagtgaa ttatctaaag
     2641 aagaagaatg tgcgacttat tatcaccagt ttaccgatca tggctgaagc aattggaaat
     2701 cctctcctag ataaattcat caaagactta atcatttaaa tctcagccat gattgctgaa
     2761 caagaacgta ctgaatcgaa acgacgccag gcacaaggaa ttaaaatcgc taaagcaaac
     2821 ggcgtctata aaggtcgtcc taagttatac agtgcagaca ccaaagatcc tcaacgtcgc
     2881 cttgtctata ggagtatcgt tcaggatctt gagaatgggg ttgctatctc aaagattgca
     2941 acagactaca atgtgactag gcaaacaatt taccgaataa aaaaagaaat cgaccaacta
     3001 attgtatgat ttcttttttg aatcacgtaa ttattaatta gctttgtcat caatacagac
     3061 aatcatttta gattcatcta gctaagttga ttacatttat gttaaaaaga atttagttaa
     3121 cataaatata attgatgcat aaatcgtcct tatttaaagg catttaacat tgacaactta
     3181 gtataatgta tcttatgtaa actaataaga tttgaatttt attttataca cactcgataa
     3241 tagatcaatt ttatggtccc ccgcatttac gattgcacaa gagaaacata aagtcgacct
     3301 attagcattt cttttcaatt caaacattaa aaaagtttat acctctgcta ctaaaaaatg
     3361 aaattatcta tttaaaattt aaaacggtaa atctgattca tccaaattgg aaaatggatc
     3421 agcctgtatt ggagctttgt atttctccat aataacagat atcttttttt cattttttaa
     3481 aaaatacctt ttaaatttct tatagtcctc tgaaatcaaa atatagcgtg atggatactc
     3541 atcaaaagaa gaatcgtatt cttcctttaa aattcccgat ttacttaatt tttctagtac
     3601 atcaaatact ctatcactca agtaactcct taaattgtta tcactttccc attgaactac
     3661 ttttctataa gttgaattat cagttgaaaa gatagaattt tcatccataa acgcatatat
     3721 tagcagcaat ctttcatcat tgtcaaattc tattggatca aaaatatcta agttgtcatc
     3781 aaaatcttta tcagttccgg taactttttt gttaattact gaaggtgtct tgtaagaaat
     3841 agaatctaaa tcaaagcgtt ttgattctaa attattttgt gctttctcaa ttaaataatt
     3901 cacttttttt gtgagaaacc ttctgatatt g
//
LOCUS       DASJTC010000151         3878 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.156, whole genome shotgun sequence.
ACCESSION   DASJTC010000151 DASJTC010000000
VERSION     DASJTC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.156"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(718..3867)
                     /locus_tag="U4B19_002678"
     CDS             complement(718..3867)
                     /locus_tag="U4B19_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEN2078339.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1076 a          803 c          637 g         1362 t
ORIGIN      
        1 ggactaatac aggtagtcct cttttttgtt attttgatat tctaaatcat tttttattgg
       61 attattccct tgttcgatat agacccaaat tgatctatca ataattatca aactgagtca
      121 tatagagaca aaaatttcaa aaatgacctg ttaaatggtc atcgaatatc atttaaaatc
      181 aatacgacgg agtcagacac ggagtcaaaa caagcccgct gagagtaaaa aatatataca
      241 gtcccatagg taaagtgcca caggactgta tattttttta tttgagatta cctgaaatga
      301 ttccatttac tttttatctc attttttcct actagttagt atcaaatact ctcctatttt
      361 ctcaataaac tattttgggt tacatggtat ctgaaacagc taaaaccttg tcattacagc
      421 aaaaaatcac tttatctccg tttcatttca gatacatttc acttaagcgg attccaaaaa
      481 aacaaaagaa aaaggaaccc ctagttagcc tatgcttttt attggttcct ctcaaaacac
      541 ggtcatataa caacggggtg gggtaaatga atgaaaaagg tggaggaaac attattcaat
      601 agctgtctta aatacttcgt tataaaatgt gaacaaataa aagacatcta tccctatttt
      661 ctacacttcc actttactac catctttgaa tcgtacaatt ttcttaactc ttctcgttta
      721 cctcaattca ttttcaagtg gaatcagctc atcatctaac attttacgcc atgctcgctt
      781 catttcttgt gggtatttaa atggcttcgc ttctggatgg ttggctttat atccttcgta
      841 atccttactg ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg
      901 cagattagaa gcatgtaatt ctttctcaga aacaaaccac tcattggaaa agacatgaat
      961 caaatcatca ctcgcttgtg taaaatagcg acgtttaatg acaaagatat cttcattact
     1021 atctaaagtc cccacatcaa acgcatttaa aatttgtcgg taaacttctt tgactccttc
     1081 tgttttattc aatttagcca aagctttttc aatttcttct cgtgcatctg aactattcgc
     1141 cgcataagtt cctagtaatt gatcaatata agtagaatca atatcataca acttcgtggt
     1201 attttcacta taaaattcga tcgtggaaaa atcttctccc tctttttctg gttcttcctc
     1261 tattaatgaa cccttcacgg tctcgtacac gccaatttgt tcttccaaaa gttgaatttg
     1321 attgttcaag gttgaagatt tcaccatgtc atcattgtaa tcgtcataag tcactaacga
     1381 ttcataagca cgattcaatt cttggaatgc tttgacgtac tcgatacgga tttcaagtgg
     1441 cgtatgttca tcaatggatt ccatggatga aacctctttt aacttaccat atgcttgctt
     1501 gaatcttttt ttggattcct gataacttgg ataaatcaaa cccgattctt cttttgcttc
     1561 tgaatacagt tttgtcgcat cctcgacatt cttctccatt gtggctggct ttcggaaagt
     1621 aacaattagt ccttttgttt tttctggata cgtacggttc gtacgtgaaa aagcttggat
     1681 caatcctgca tactctagat tacgatccac aaatagggtt tgaatcgtag gtgcgtcaaa
     1741 acctgtcaat agacgatcca ctacgatcac taaatcaatt tgtttcccaa attgtttgaa
     1801 ttccgctctt tttcgtgcca aacgattatt gatgtctccg ttataacgct caatgtctgc
     1861 taatgaccag gaagtatcat aatactgatt atattcctca ataatttttt gcatttcttc
     1921 ttgttggaca cttgaatctc ggctgttttc ttctaatgaa taggtaatag cgattcgtgg
     1981 aaaatcagga tcctccatcg ttcttccttc acgaatgggc tgattaggaa actcttttgt
     2041 taaccagtca ggttctttcg tcatttcttt gatcgctcga taatactttt tggccatgtc
     2101 aatggaactc gtggttaaga tcgcagactt cgttggacgg ccatttcgaa aatcaaattt
     2161 tgtataggca ttgtctggac ggaacatttt atgaatgact ttttggatat gttcatctgc
     2221 ttcaaaaaca gtagggtcca aataactttc ttttttgaca ggctccattt gatcaatcat
     2281 acgattgatc tgttccggtg aatactctgc atatgtttct acttctttta atttccgata
     2341 gattttattc tctaatgaag tcggctccac ggtgttttca tgctctactt gaaatcctaa
     2401 aaccgagcca tcttctaatg cattcttgat ggtataggta tgcaagacct ctccatactg
     2461 atcatgggtc gtacgtgcca attgcccttt ggcttgtttc cgattctcag ggaaaatcgg
     2521 tgtacctgta aagccaaacc aagtggattt cggaaacatt tttttgattt cttccatatt
     2581 ttcagcactt aacgctcgat gacactcatc tactacaaac acgatatgtt gccccattaa
     2641 tttttggaag cgattggttc cttttttctc ttcttgtttt tttgcatatt tcaacgccgc
     2701 atctagcttt tgacgggttg taataatcac aacatttgca ttggcatctg ccaacagagt
     2761 ttcacttaac tctttggcac ttcctgttcc cacgatcaag ctattcgctt tggcgttccc
     2821 ggaagaaatc ccagtattaa attccgaagc aaatttcgta aactctgtgg tggtttgatt
     2881 atctaaatct ttgcggtcca ctaacatgat cgtccgatcg atccccgatt ttcgtgctaa
     2941 aagcttagta gatacaaaac tagtcaacgt ttttcctgaa ccagtcgcat gccacacata
     3001 tcctgattga tgtttatttg cagcaataaa caaggcttga atcgcatgta cttgataagg
     3061 atgtaacacc atgaggattt tattgtcttg atcttcactg acaatcgtat aatccgcaat
     3121 caagcgatga gcatccggga tatttagcac ttgtttgcaa aactcatata agttttctac
     3181 ctttttatta tctttcgttc gccaactaaa taaaaactta ggatgcaaat cctttggtaa
     3241 ggcatttgca aaataccgag tggtttgttc attggaaacc acaaacaact gtaaggtcga
     3301 aaagatattg ttccgaaaca taccttcttc tgcatatttt ttgatttggt taaacgcctg
     3361 ataaaaacta tcttttgcgg ttacttgttt cagctcgatt tggacaatcg gtaatccatt
     3421 gatcagtaag gtgacgtcaa aacgtcgatc ccgtgcttct atgttacttc cacgttttgc
     3481 aatttgatgg accacttcat aggtagagat tcctccacca atgtcttgat tagaataaag
     3541 aatgagcgag acagaaccca gttgcggatc ttcccgttca atcgtgatcc gagccattcc
     3601 attttcacct tttaaccatt tcgcagcatc aaagggggtt ttcgttcgta gtaacaattc
     3661 tgttttgatc gtttcaaatt ctttatcggt caaaggaaaa tcattcagtt ctgcttgatt
     3721 atttgagatg attttttggc gtaaattgac ccaaagatct tcttctgatt ttagatcggg
     3781 acgatacgtc cactgattat gcccttcccc caatacacgg atcaactgtt cttcgacttc
     3841 cgcttcctca cggtgactaa ttttcattta tcttccct
//
LOCUS       DASJTC010000152         3875 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.157, whole genome shotgun sequence.
ACCESSION   DASJTC010000152 DASJTC010000000
VERSION     DASJTC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.157"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(151..990)
                     /locus_tag="U4B19_002679"
     CDS             complement(151..990)
                     /locus_tag="U4B19_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEN2078340.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1175..2062
                     /locus_tag="U4B19_002680"
     CDS             1175..2062
                     /locus_tag="U4B19_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEN2078341.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(2656..3350)
                     /locus_tag="U4B19_002681"
                     /pseudo
     CDS             complement(2656..3350)
                     /locus_tag="U4B19_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(3334..3732)
                     /locus_tag="U4B19_002682"
     CDS             complement(3334..3732)
                     /locus_tag="U4B19_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEN2078342.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1318 a          733 c          582 g         1242 t
ORIGIN      
        1 atataaaatg aaaatgatga gaacacgaca gtcagttgtg aaaatctaac aaaagatgtc
       61 gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa tgacagactt aaaaaagcaa
      121 gaccgatatc agccttgctt tcttcatctt ttattgataa acagcagtgt atttcttcat
      181 ccgctttgtt ttatatgcat agagtaacaa accaacaatc aagactaaca agaataatgt
      241 agttgatata gatgcttcag ccccaaagtt tcccccagta attattgtat tttgcgattg
      301 gatcagagtg gagaaaaaac cttgttcttg cgttccgctg actggtatac ccaaaatagc
      361 tccttgtgtc caattccaag taatatgagc accaatagcc gtaaataaac ttcctgtact
      421 gtagataagt gcgctgaaaa agcaacctgc taaaaagata ttgaaaactg ccagaaatgt
      481 catgttagga ttcaatccgt gcaaaagagc aaataatgca gcagatccaa tcattgctat
      541 taacggacca aatctaagca tctgatgctg aaaaaagcca cggaataaaa gctcttctga
      601 tgcccccact gcaataaata aaataaaact cgttatcagc ggcacaatgt ttgcgtttcc
      661 tgaatctaac cgatacgccc ccgtcaaata taatggacta gtaataagca gaaacactcc
      721 aattccaaca acgagacctc ccgctaaata cccccagtct tttcctcgaa cagaaacacc
      781 ataatcttct attctttttc cttcgacctt actagcaaga aataaagcta gtaaaaaaac
      841 agctaaatgt ccgaccgtac cggcaattgc tccaatccat tcactatttc caataaacat
      901 atccgcaagt gcattattga caagaattcc taaaacaaaa ccaacaaaga ctattaaaac
      961 agatccaatc aaccgaataa tcctgatcat ctttcatccc tcctttatat taagtttaac
     1021 aacccttttc tttcttgtct cgtgaaaact atgtaaatgt tctccatcat agagagaata
     1081 gatttttacg aactaagaat atctacagca accttacgaa aatgaaagac tgtctcccta
     1141 attcttctgt tagactgttt ttaataagga aaggatggga aattttttga aaaaacaact
     1201 actaattatg tgcctttcag catgtgcaat tttactattc tcaggttgta gcaagtccaa
     1261 tgaaacgatt gcaacgttta aaggtggaac acttacagtt caggattttt atgataaccc
     1321 aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg attgtcttca aagcatttga
     1381 tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca aaagaatata atgaacaaat
     1441 caaaaaactc ggtccaaact acaaggaaca attaaaagca gttggccaaa cagaagagac
     1501 atataagaaa ctctttaaac agatgctggc tttccaatac ggtttgaaag ccaatgtaaa
     1561 attaacagat aaagatctag acactgcttg gaaagaattc tatccagaag ttagtacaca
     1621 gatcattcta ttttcgacta aagaagaagc agataaagca aaaaaagaag caaatgaagg
     1681 agaaaatttc tccaagcttg ttcaagctta tggaaaaaat aaaacattaa aggaaactga
     1741 tggaaaaatg aattttgatt caaccaatcc agagattcct actgaagtaa aaaaagcagc
     1801 atttaaacta aaaaacgggg aagtttctga tatcattcct gtaacagatc caacgactta
     1861 tcagcaaagc tattatctag taaaaatggt aaaaaaacag gataaaggtt ctaataaaga
     1921 caaatacaaa tctgagctag aaaaaattgc taccgaagca aaaactcttg atacggaatt
     1981 catggataaa acaatccgta aagtgatgaa aaaagacaat gtaactatta aagatcctta
     2041 tgtaaaaaat atattcaaat aaacaaattt gacccaattg gggatgtggc aaaatttgag
     2101 aagtgatttt tggaaattcc ttcttatttt tgaggcaaac cacttttgcc acaacttcat
     2161 cggggccttt ttttatttgt accaaaacaa atcactggat ttgttacaat catgaggata
     2221 taatttaaaa aagtttttta tttctatcac atgaatcaat gcgccattaa gtagattctt
     2281 tcatgcttcc ctattttttc acttgccgta tcgcttcaaa aacaactatc ataaactcaa
     2341 actcgactga aaagttattt taaaaaagac taaaaatatc ccttctggac ttgttcttct
     2401 tattctgtta tactagagaa acacgaaaga tatttttatg cgacactttt catttgtttt
     2461 tatgaaattt attcaaaact ggcgtaactt cagagttaca aggatgtatt agaggttagg
     2521 gaatacatcg tttaaatgag aaataagtaa tagttagtaa tagtcaccca aactttaaat
     2581 taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta tcgtttattt
     2641 tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa tcgtttctgc
     2701 ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa tcgagggcat
     2761 ctctaatact tgatctagta gctctttacc taacgttatc gctgagctcc agatcgaatg
     2821 gctgtagtga cttgttcaca atttttgaaa ctagctgcta atatttttgt tggtctttgc
     2881 gtacgttcga tctcagtccg caacgaatca atcactttca tagcatcaat gccttgattt
     2941 tgcatcctgt tataataagg tgcaatataa tctgctccat tattaatagc taaataccct
     3001 tgtaattctg tgtaaatagc tgtaccggta atcaaataac cttctttttt caactttttg
     3061 atcgcttgaa ggcctgcttg attcaccggc accttgataa acgtatcttt tcccagttct
     3121 tctatgataa ctcgagcatc ctctatcatt tcttcttccg ttcttcctat tgtctgaata
     3181 tggataggaa tctgtccgat tatttttttg atttctttca tatgcgagaa gaagtttgac
     3241 gctccttctt ttttcagaat tgtaggattt gttgttacgc ctgttaaatt caaaaactgt
     3301 tgacaagaat aaatactttt tgtatttgct gtatcaagca tgatttccat atagctttcc
     3361 cccattctga tcctcttgat cgattttaat gattgtcccc tttcttaaat gcggcatttc
     3421 cttatcttct acatacattg ttcctggaag ttccggctca tgagatccat caaattttat
     3481 cgtgatatgt cctaattcat tcaaattagt ttggacaaca tctcctactg ctgtgattac
     3541 atacatttgt ttatcaaaac agagcttcat atcacaagtt atttcttttc ttacctgatt
     3601 caattcaatc ttataacaat aatctgttaa tgtttccggt gcttcatctc caaataaaat
     3661 cattattttt tctttcctga atacttctgc ttctatacca acatctgtta caaccgtttc
     3721 aaagatcgtc atctctgttc ctcctcgtaa aatatcattt gtctaatagt ttttattcaa
     3781 aatgtttcta gattataaaa tttattgaat aacacattcc taacggaatt tgtttcgata
     3841 ttgtgtgatt tctcagagta caatggatgt atgga
//
LOCUS       DASJTC010000153         3799 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.158, whole genome shotgun sequence.
ACCESSION   DASJTC010000153 DASJTC010000000
VERSION     DASJTC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.158"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            154..237
                     /locus_tag="U4B19_002683"
     CDS             154..237
                     /locus_tag="U4B19_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEN2078343.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            362..1099
                     /gene="erm(B)"
                     /locus_tag="U4B19_002684"
     CDS             362..1099
                     /gene="erm(B)"
                     /locus_tag="U4B19_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN2078344.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1362..2225)
                     /locus_tag="U4B19_002685"
     CDS             complement(1362..2225)
                     /locus_tag="U4B19_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2078345.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2227..2499)
                     /locus_tag="U4B19_002686"
     CDS             complement(2227..2499)
                     /locus_tag="U4B19_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN2078346.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2516..2725)
                     /locus_tag="U4B19_002687"
     CDS             complement(2516..2725)
                     /locus_tag="U4B19_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN2078347.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2803..3699)
                     /locus_tag="U4B19_002688"
     CDS             complement(2803..3699)
                     /locus_tag="U4B19_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2078348.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1165 a          670 c          583 g         1381 t
ORIGIN      
        1 gattcatcgt cattcctcct gctatctttt tctattattc taccttattt gatagtagat
       61 ttaaaacttt gcaacagaac caaaattttg tataatagga attgaagtta aattagatgc
      121 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgtaatgta
      181 gataaaacat ctaccgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      241 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      301 acaaacaaat cgtttaactt ctgtatttat ttatagatgt aatcacttca ggagtgatta
      361 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      421 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      481 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      541 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aacactcgtg tcactttaat
      601 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      661 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc
      721 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
      781 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
      841 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
      901 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
      961 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1021 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1081 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1141 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1201 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1261 aaattcctcg taggcgctcg ggaccccaac atgaggaaat gtttttataa aaaaagagta
     1321 gttaccgttt tttggtaact actcttttcc ttttttaagg tttaaatacc tggaagttta
     1381 ggtgttttgg gtattggtgg tgtaggtagc tgcaagcttt ccattttttg ttgaattgct
     1441 ttaaattcag gggtttcttg gtgttgattt tgaaccattt tttgctctat tctctccaaa
     1501 gtcggttgaa tttctttccc tgatacttta cgatttaatt ctctttctaa ggtctctttc
     1561 ggactaatgg aaggcgtttc taagcttgaa tagagtttca ctccttctct attgtaaagc
     1621 cttatatcgc taaataagcc cgttttatga agggtttcta aattggtcgg taagtttttg
     1681 acaacaatat catgcgcttg ttttggtgtt gccctagctg tcattggatc atctgcatac
     1741 atggtttcat atcgttcaat cgttcctaag tatgattcaa ttttcggtac tgccatgacg
     1801 tatatttttg tttcataacc tttagattga agcattgtgg ctgtttgaat gggaacgtct
     1861 gttgttcgtc ctgttccttc aatcactaaa ttatagcctt gatcgcttaa acggctaata
     1921 agtgcttctg tcatgcgatt cgaataaggg gtaacgtgtt ttactacgtc tttttcatag
     1981 agtttcacta attcgtcaaa atttgggtgc tgttgtttaa aggtatcatt atcaatgaca
     2041 ataacattcc cttgtgtttc ttcaaaaatt gctgatcgca aactggtttt ccctgaccct
     2101 ggttgcccac caagtaaaaa agcggttggc gattcaaccg ctagtctatt tttagttagt
     2161 cgttcaacat tcttttctaa acgatcttca aattgctttt cggtgaagtc agtaatattc
     2221 gccatgttaa gccactttct ctttattcaa aatttgtttt gaatattgat tcattgaccg
     2281 ccaatactca tgaatagctt gtaattcttc aaatggttgt tgaaataaat caacttcttg
     2341 tgccacttct aattgattta aaagattcac ttctagtaaa cgagtatttt tagtatctag
     2401 ttcatgattc aaaacataat ttaatacccg attataaaca cttgccgata actcattaat
     2461 gatttcaatt tcaaatgttt tttcatacgt aactgccatt tcattttcac tccttttaaa
     2521 gtttgtcagg taaatatttc cgtatatatt cttctaaggc ttgatccata acttctttta
     2581 cgtttccgcc attctttgct gtttcgattt ttatgatatg gtgcaagtcg gcacgaacac
     2641 gaaccgtctt atcccccatt atatcttttt ttgcgctgat tggcgtatca tttcgttttg
     2701 ctttctgtgc gcctaaattt cccacgatcg ctcacttctt tctatttctt cttactattt
     2761 attttatcat cacctatcac aaatcacaag tgatttgtga ttctattctt tttcattttc
     2821 taattgaatg attcgctcaa tcatttcaaa aaatacgttc tcatacatag acaaaacttt
     2881 tttgtcatag cctttgtgtt ctgtaatgcc atttttagac caagtactta ctttattact
     2941 tcgcttgata atatttcgga aaaccaaatt atcctcttta tgttctttgt acagttcttc
     3001 caggtttgat tttatcgttg cgctgtccgt atcaactaaa taaggaacaa aaccaatcat
     3061 atctagtcca gggttaaact gttcttgcaa atcaatcaaa taggaaatat agttttgaat
     3121 gttgtttgta ctttcttctt ctgcttgtaa agggatcata acgtaatcac tcgccacgat
     3181 tgcattattt gtataaacgc ttggcgttgg tacagtatca ataataataa gatcatagtc
     3241 actttttaaa ggtgctaaaa gagtagcaag caatctactt tcattttcaa acgtccatga
     3301 gcgagttaat tttggcagta acatcaaatc aaacgtgcca gggatcaagt ctaaattatc
     3361 agtcaaatga ataatagaag aagccaagtt tccatttttc aagccttcat aaaaattgac
     3421 acgtggcaat tctacctcaa aagtttttgc taagtctttt gtcaatgttg cctgtaagtc
     3481 cttatcgatc attaaaactt ttaaattcaa tttgtctgtc aagtaagcaa acatagtcga
     3541 taatttggac tttccaacac cacctttaaa gtaattattc aagataataa ttgcttcatt
     3601 tttattgttt aaaatacgtc ttaattcttc aagtattttt agtttttcct tctccataac
     3661 tacgccccat tctttttttg tatagtaata ttgtatcatt aagaatcaca aatcacaagt
     3721 gatttgtgat taatcacttg tgatttgtga taagtgattt gtgattaata tataaaagcc
     3781 ctctttaaag ggcttttat
//
LOCUS       DASJTC010000154         3718 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.160, whole genome shotgun sequence.
ACCESSION   DASJTC010000154 DASJTC010000000
VERSION     DASJTC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.160"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..353)
                     /locus_tag="U4B19_002689"
     CDS             complement(<1..353)
                     /locus_tag="U4B19_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078349.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="U4B19_002690"
     CDS             complement(613..1161)
                     /locus_tag="U4B19_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078350.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="U4B19_002691"
     CDS             complement(1162..2019)
                     /locus_tag="U4B19_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2078351.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2591)
                     /locus_tag="U4B19_002692"
                     /pseudo
     CDS             complement(2305..2591)
                     /locus_tag="U4B19_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2581..2910)
                     /locus_tag="U4B19_002693"
     CDS             complement(2581..2910)
                     /locus_tag="U4B19_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2078352.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2987..3259)
                     /locus_tag="U4B19_002694"
     CDS             complement(2987..3259)
                     /locus_tag="U4B19_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEN2078353.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3259..3522)
                     /locus_tag="U4B19_002695"
     CDS             complement(3259..3522)
                     /locus_tag="U4B19_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2078354.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
BASE COUNT         1091 a          754 c          473 g         1400 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagacg aatcagattt tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gctttttaaa tacttaatga catgttttga aggaacgatt
     2581 tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat tagtaagtgt
     2641 atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct taaattctac
     2701 aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat cttcgctaaa
     2761 atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct ctaccaactt
     2821 tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca acatgattga
     2881 attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac agtatgtaat
     2941 tacttagtaa atacattata ccacgagata atgtatttac aagtaattac tgtgactcgt
     3001 aatgcgtcct tgctgaataa atttcaacca cgtgttcatc ttcatctacc ttataaacaa
     3061 cccggtgttg tctattcaat cttctagaat acctaccttc atgtgtaggt tttaacttct
     3121 caaatgattg ggtagggaga tagggatctt cctttaaagt ctgaataacc ttctcaaatt
     3181 gaggtttcaa attagactgt ttgattttct ttaaatcagc tttagccgag tttttaatca
     3241 tcacagaata gttattcatt ataagttatc ccaatcaatt tcatcaacat tagtaaatcc
     3301 actatcatct ttttctcgct tacgaacaac acccaaagtg cctatttgct caagtaatag
     3361 cgtttctttg attgctttcc aatcatccaa accaataagc accgcattat tttcactgcg
     3421 ctcactaatg atttcaattt ccgtatgatt ctcatttaca tctttcaaca acttataaaa
     3481 atttttccgt gctgagctag gatttaagac tgtttttgcc atatctactc cccctctaat
     3541 ttatatctcc attctaacgt acttaattaa gtacgtaaat tcgattgttt tgtttaaatt
     3601 ggagtaccaa aacagcattg taaagtgtta tccgtttcct ttatacccgt ggtgctagtt
     3661 aattttttcc tagaggaaaa tgtctaaaca tgttggtaaa tcaacaaaaa aacgcata
//
LOCUS       DASJTC010000155         3705 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.161, whole genome shotgun sequence.
ACCESSION   DASJTC010000155 DASJTC010000000
VERSION     DASJTC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.161"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            64..972
                     /locus_tag="U4B19_002696"
     CDS             64..972
                     /locus_tag="U4B19_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEN2078355.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..1592
                     /locus_tag="U4B19_002697"
     CDS             972..1592
                     /locus_tag="U4B19_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078356.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1596..2045
                     /locus_tag="U4B19_002698"
     CDS             1596..2045
                     /locus_tag="U4B19_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078357.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            2045..2539
                     /locus_tag="U4B19_002699"
     CDS             2045..2539
                     /locus_tag="U4B19_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078358.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            2553..2984
                     /locus_tag="U4B19_002700"
     CDS             2553..2984
                     /locus_tag="U4B19_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078359.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            2986..3123
                     /locus_tag="U4B19_002701"
     CDS             2986..3123
                     /locus_tag="U4B19_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEN2078360.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            3160..3453
                     /locus_tag="U4B19_002702"
     CDS             3160..3453
                     /locus_tag="U4B19_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078361.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            3450..3674
                     /locus_tag="U4B19_002703"
     CDS             3450..3674
                     /locus_tag="U4B19_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEN2078362.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT         1334 a          551 c          819 g         1001 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taagaataca gaggttatgg aagcgactat agttgaaaat
      181 ggtcttccct acgatttatc agaatgtaag gtatattttg aatcagttgt aggtgggaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat taactatatt
      301 tttgataaat attccatgca gtgtttacat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcattaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaatcg aagatttaat tgcggatatg
      481 acggacttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgaac gttggagaca agaacaggaa cacagtttcc aagattggag agaaggacaa
      601 gaatcagact atttggcatg gtttgaatca atcaaggata ttttaaagac gattgatcca
      661 ggtggagtca tgttagccga actaacggat gcgcgagtag atattcaagg agttcgtcat
      721 gcgtctattt cagagcgttt gctggcagat atggactatt tatatcagaa attgcgagca
      781 acgcttttca cgattgaata cggggagatt gaagtgactg atattttgca ggatgatctc
      841 ttttcagata atcatgaagt cgaaaaagtt ggaactgtag cattcccgat tgaagaagga
      901 gccttgatca tcgcaaccgt tgatgatcca aagcaaaatg tgttcactct tgagaaagtt
      961 ggggtgatct aatggctaaa actaaacgaa tgatggaaac ggatgaaaaa acgggtgtac
     1021 aacgccaatt ctttccaatc acacatgctt ccgcagttct tgggttagaa gaaataatag
     1081 caggagaagc aacagtttta tctgttaatg gaaaaattgg agccgtcgtc attactaaag
     1141 aagatttggg attagagaat gttctcacag aattacccta tgcaagtgaa gaagatgacg
     1201 gcattatcac ggcagaaatg tatcaaaaaa ttttaaacag tggagaaggt gactacgtgt
     1261 tgccagttgc cactatcgac cgtttgggtg gcataaaagt tggtgaatta ttgacgattg
     1321 atgagacagg gaaagtctct gcagtcagac aatctgatgt caatttttcg ctggagttaa
     1381 aagaaaaact cgattcactg aaaaattata ctgctggaga aaacatcact attgatgaag
     1441 atggaacaat tagttctaca ggtggtagtg ggactggtgg tgtcaatcag tcatatgtag
     1501 atcaaaaatt ccaagaagct gtaaatcaag cagaaaatta cacaaacgaa agaattccaa
     1561 attttacttt tgaaaaaatt ggggaggtat agagaatgac agatatcgta aaaataaaac
     1621 aaagcaatgt acaggtttat ccacaaactc attggaatgc tatagaaggc aaaccaacaa
     1681 cggtgaaggg agataaaggc gatcctggcc aagcagcaac aattaccata ggaaccgttt
     1741 ctagtggttc aaccgcttct gttacgaatg taggaacatc atctgcagct agatttaatt
     1801 ttgttttacc aaagggagat aaaggtgatc ctggaataaa tgccacaact acagctgtag
     1861 ctacaactac agcaaatggg ttgatgtcgt ccacagataa aactaagttg gatggaattg
     1921 cagctggagc acagaaaaat ccaggtaatg ctacaactac gacagcaggt ttaatgtcag
     1981 caaccgataa agtgaaactt gatggattag cgaatattac atttgagaaa gtggggacgg
     2041 tttaatggct gatattgttc aattaaaaga agacggagtt gctaaatact taaaaacgca
     2101 tgtagaagct attgatggta aagaagcatt ggtgcagaca gatggagacc aagccattgc
     2161 aggacataag aatttttcag gttctgtaac aatcaataat aaacgtgttt tgacgactga
     2221 tgataacaga tatgaagtgg taaatctggt tgtcactaac ggtaatacag ggacagcaaa
     2281 gctttatcgt gaaggaaaaa cagtttctat atattttgtg gctttaaacg gaaaaagtag
     2341 tggcgggaat gattcggtta ttttaactgt tccagaaggc tatcggacac caattagttt
     2401 tgaacaactg gttgggtcaa tagaccgttc tactttgaac agtgctcaac tatctattgg
     2461 cgcagacgga gccattaaat ggagaagaaa ttcaagttat ggatcagctt attcatttgt
     2521 tatcacttat tcaatttaag ggaggaaatc taatgaaagt agtttacaaa tcaatcaagc
     2581 cttacggatt cgagcaaatc attttgaaca atcaagaaaa tatccctgaa aactgtacag
     2641 agattaaacc accagttcct aactggagac caagatttga ttttgataaa aaacagtggg
     2701 ttgagttagc tactgaagaa gagaaaaaag ggaatgctgt tgatagcgta gatgaattgg
     2761 ctaatattaa ggcattatac gaaacgctga aggcagaaaa tgatgaattg aaacagctaa
     2821 attctaaagc aatgctaaac aatgtagcaa tcaagcaaga aaatgtctta ttgaaagaaa
     2881 agtcagaaag tttagcgcag ttgaattcaa aaacgatgct tgcttctgta caaaatacca
     2941 aggaaatcga agaaatcaaa aaacaattac aaggtgggaa gtaacatgta ttcatatgaa
     3001 gatatcaaac taatgtatga ctggggctgt ttcactaacg aacaagtaat ggttttcgtt
     3061 ccattatgca ttacagacga agaagcagat aaaatcatta ataaagataa gagcgcatct
     3121 taattgatgc gtttttattt aaggtaaagg agttgtcaca tgattaattt aggggaatgg
     3181 ggaatgatag caggatcaat aaccgctatc gtttctttga ttttattagt aattagaccg
     3241 attgctgcat ctttctcgaa aattactgag actctttcaa aagtaagcca caatttagat
     3301 ttgctgacta aagatttaga atcgagcaaa tcagatcgat tgatgattca tgaagaacta
     3361 aagaaacacg atgaaagatt agatacacat acagaaaaat tggtggaaca cacgcaacaa
     3421 atcaaaactt tgtttaggga gagaagaaaa tgaataataa aacgttcgaa gtactaaaat
     3481 ggttcgcact ggtaattatt cccgcactag ctactttcgt ggggttagtt ggtaaagcgc
     3541 tcaattggca gtacacagat atctgtgttg tcatcattac tggttttggc gcgtttttag
     3601 ggagtgtgtt gggtgtatca aatcgaacct acaaaatgtt ctcggctgaa agcgaagaag
     3661 gaggaaacaa atgaaaaaga aaattactat tactgcgatg agcct
//
LOCUS       DASJTC010000156         3697 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.162, whole genome shotgun sequence.
ACCESSION   DASJTC010000156 DASJTC010000000
VERSION     DASJTC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.162"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..337
                     /locus_tag="U4B19_002704"
     CDS             <1..337
                     /locus_tag="U4B19_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078363.1"
                     /translation="SVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKS
                     ISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNN
                     NFYFYRNKL"
     gene            complement(463..1140)
                     /gene="nth"
                     /locus_tag="U4B19_002705"
                     /pseudo
     CDS             complement(463..1140)
                     /gene="nth"
                     /locus_tag="U4B19_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(1147..1845)
                     /locus_tag="U4B19_002706"
     CDS             complement(1147..1845)
                     /locus_tag="U4B19_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEN2078364.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2113..3297
                     /locus_tag="U4B19_002707"
     CDS             2113..3297
                     /locus_tag="U4B19_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEN2078365.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3303..>3697)
                     /locus_tag="U4B19_002708"
     CDS             complement(3303..>3697)
                     /locus_tag="U4B19_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2078366.1"
                     /translation="IIHRNKPEMEKEFAGFTLNELEVIEHIGKIPDPNVTKLANASYM
                     TRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKIRNRHEQLHQKWVQRDAT
                     VFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT          954 a          733 c          623 g         1387 t
ORIGIN      
        1 ttctgtactt ttaagcacta ctcttaaagg aaaatttatg gaaagaagct ctcatgcaat
       61 gattctcact tttttatggt tgttgttcac cagaatctat atttttctga tgagcctgaa
      121 accgcccaaa tccattagtt tatgtttagt tataaatggt atttttttat tatcacttat
      181 catttttatt acacctttag atagatacgt cacttattta tataatcctt tggaatactg
      241 gacttatttt ataggtatat tggaaggcat tattttctac atcgggtatt ttcccaataa
      301 aaataacaac ttttattttt atcgcaataa attgtaataa gttaccatcc tagctactga
      361 gtatgtacat ttactttttc atgcaaaaaa gataaacgaa acagcttttc tccataataa
      421 caagataatt catacattta tttgcaatta aagaaaccac gtctacaggc gtggtttctt
      481 ttttagcgcc ttttctttta gacgcatccg atttttccca tcttgacata ttgatagcaa
      541 aggacatacc tcgcacttgg gatttcttgc cgtgcaatga tatcaaccaa aaaaaatcag
      601 tgtatgatgt gtctttaccc ataattcttg tggtactttc cgcatcagtg tttcttctac
      661 ttccatgacc gtagcatcta gtttgcagat cctcaggcgt tttgagacac gttccacgtg
      721 ggtatctact gcgattgctg gtatcccgaa tgcatccccc aatacgacat ttgctgtctt
      781 tctgcctaca ccaggaagag acatcaattc ttctcttgac gttggcactt gcccatcgaa
      841 acgttcaatc agctgctgtg cacacgcttt gatgtttttt gctttatttc gatacaaacc
      901 aatcgttttt attttaagaa tgatttcatc gattgaagct tctgctaacg catcgggtgt
      961 cggaaaagag gcaaacaaat ctggcgttgc tttattcact gatacatctg tcgcctgggc
     1021 acttaagatc actgcaatca ataactcaaa tggattattg tgcttgagtt ctccatgtgc
     1081 ttcgggaaac attccataca tcgtttctag agcttccatt gttttttgtt tgcttaacat
     1141 attccgctac tcctccaacc aatttttcag tgagactttc ggcaattctt cttgttgagc
     1201 tgactgttct acttctcttt gaagaagctg ctgttttctt cgtttttggt cttcttccac
     1261 ttgttgtttt gtacgtagat tttttctttc ccacgatagc aaaatgcggt caacgtaatt
     1321 aaaactataa gcctgattta atacggcttc ccgcagtgct agcttcaaaa tttccggctg
     1381 ataatggtct tcttctagcc attgtccgat ccgctggaac tcgatcgaag aaagcgggcg
     1441 tccaaattct tgttcaaata attgatacac tgactggatt tctttttctt gtgttttttc
     1501 ttcgtatttt ttttcttgtg tttttaaata ctcacctaat agatcataga tggggtaaag
     1561 attatatcga tctgcttttt taccgttgac aatcgtcgtt tctatcttga taaatccatt
     1621 tgttacgaga cgatccagta tttgaaaaat ctgatcttgt ttgattccca tatccatagc
     1681 aatcatctgc aaatcaggga aagtatctcc ttcaagctgt gccatatgca actgtagaac
     1741 aaataaaaat tcttcagatt gtagaccgat cctatgataa tttttaagta aaagattaga
     1801 tatgactgtt tgtccagcgc ttaagtaatt ttctaaattc aacatgattt ctcctccgct
     1861 caatcacagt atacgtaaaa aaaacatggg aagcaagaac aggtttcttt tctatttact
     1921 tcactgatcg gtcagattta caatgtcgtc tacttgattt ttgtttgttc atgtttttga
     1981 tgcgtatctt cacttttcgg aataaataaa ttaagtagat ttcgctgttc cttagaatgt
     2041 tgacaaggaa acaaaattac tatacactat ttttgtttcc aagttaacat tattgcataa
     2101 aggagttttt acatgtacag tttcacaaat aaacattcta aagtttttgg atacgtctct
     2161 gtcttattca ctggattagg atttactatc gtcagtcctg tacttccgtt tttaacgctg
     2221 ccttattctc actccataca tcagcaagct ttctatatca ctttgctcat gtctgtttat
     2281 gctctagctg ccttcttatc tgcccccatt ctcggtagct taagtgatca ttttgggcgt
     2341 catccgatcc ttattatcag tttattagga tcaagtatcg gatatttgct ttttggttta
     2401 ggaaattcga tatggatgtt gttccttggt cgtatcattg aaggattgac ggcaggagaa
     2461 atctccacct tatatgctta ctttgccgac atcacggaac ctaatgaaag aaccaaagtt
     2521 tttggatgga tgggtgcttt agtcggtata ggaacaacgc tgggtcctat tatagggggt
     2581 ctattagctg agttggggaa ttctgttcct atatttattg gagcactctt tactttctta
     2641 aatgccgttt atggctacac atttatgcta gaaagccttc caataaaaaa acgttcagta
     2701 gatttaagtt tctcccatgt aagacctttt caccaattac aacaattatt caagatttca
     2761 tccgttattc ctttattgac agcaggtttt gctgtttggt tggcagctgg ctctcttcaa
     2821 tctatctttt ctcaattttc gattgacgcc tttcagtgga aggctgggtt ggttggttta
     2881 agcttctctt tgattggaat cttggatact gtctcgcaat tgttcattat gcctcgattg
     2941 ctcaaaaaat tttcagaaca gcagatcaca agaataggga tgttcagtga aatcctcgcc
     3001 tatctattca tcacattatc tggaattctt ctactgccga ttttgttttt atttgggatt
     3061 atctgttatg gtttcggtga ttctattttt actcctgtat tcaatggcca actatccaat
     3121 tctgtctcag ataaccagca aggtcttgta atgggcggta cccaaagtat acaatctcta
     3181 tcgcgcgtca tcggcccgct aatcgcagga cagctttatg ctgcagcacc atttcttccg
     3241 acggtattcg gatttttgct tatgattttt gcctattatc actacaataa aaaatagtag
     3301 cttcatttgc tacttcgtat aaattctctg tatcgaccga taaaacgaaa tactgtgtca
     3361 aattcttctt ttgacatatt ttcaaataca gtagcatctc gctgtaccca tttttgatgg
     3421 agttgctcgt gtctattcct tattttttgt ccgattggcg tcaattgaaa atagatttct
     3481 tttttattct cttcgctttg ataagtgttt atgatccctc tagctatcag tttttttgtt
     3541 agcttactga tggctcctct agtcatgtaa ctagcattcg ctaattttgt cacgttagga
     3601 tcagggattt tcccgatatg ttcaatcact tctagttcat ttagagtaaa tcctgcaaat
     3661 tctttttcca tttctggttt attacgatga ataatct
//
LOCUS       DASJTC010000157         3566 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.163, whole genome shotgun sequence.
ACCESSION   DASJTC010000157 DASJTC010000000
VERSION     DASJTC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.163"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(2..982)
                     /locus_tag="U4B19_002709"
     CDS             complement(2..982)
                     /locus_tag="U4B19_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEN2078367.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(1006..2490)
                     /gene="galT"
                     /locus_tag="U4B19_002710"
     CDS             complement(1006..2490)
                     /gene="galT"
                     /locus_tag="U4B19_002710"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEN2078368.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(2502..3491)
                     /gene="galE"
                     /locus_tag="U4B19_002711"
     CDS             complement(2502..3491)
                     /gene="galE"
                     /locus_tag="U4B19_002711"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEN2078369.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT          928 a          777 c          648 g         1213 t
ORIGIN      
        1 tttatttcgt tgattctcga ataatcaact ctgtttccac aataatcttt cttgaaaccg
       61 gtgctttgga attacttaat tgaagaatcg tctccactgc tagctctccc atccattcgg
      121 tataaacttt tacagtagaa agagagggcg ttacatattt cgctacactg acatcattga
      181 aacccatcac agaaatatct tctggtacca ctaaaccttt ttcttgaatc gctttcagtg
      241 cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc aggaacatgc ggattcgttt
      301 ttaaatactg cttcactgct tgatatcctg actcgacaga aaaatctgct tcgataattg
      361 gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa tgccataatt ctgggatctt
      421 ttagccgctg atggtttttc ttcgtatatt ctgttccagc aagcatagcg atatgttgcg
      481 ctttatggga gacaaaatgt tcaatgactt tttgaacact tgacttaaag tcgacaacta
      541 gagagtcgat tccttgagaa gtgccgtcag aatctaccaa aagcaaggca ttttgggatc
      601 ctttcatttc taataactga gcttcgtcga attttcctag tgcgatcgtt ccatctgtcg
      661 gaagatcact aatggaatcc cacgtttctt tcaataaagt aacgtttgct tcttctgcct
      721 ttttttcgat tcctagacga atagataaat aataaagatc ttcaagctct tccgtatcgt
      781 cgtaccactg gatcaggcga agcgtttgct tagtatttgc tgcattttta cgatgtttcg
      841 tataatttaa gcgttctgct gcttcaaaaa ttttttgctt tgtggctaag ccaacggaaa
      901 gatcaggatc atagttgaga actcttgaaa cagtagcagg agaaactcct gccaactgcg
      961 caatgtcttt aattgttgcc atttgtctct cctccttttt tacgtttata aaattgcagt
     1021 aaatcgttca aacgctgcct gtcctttttc atctcgcttg aatactccag cgtcttccag
     1081 tacacgtgca aaaacctgac ctacagattt ttgtacgaca tcggtgaccg tatctttcgt
     1141 caaatcagga tgttcttttt ttaattgttc tgcccacgga cgatgatatt cagccatctg
     1201 gttttcttct cctaataaga aatgctctac ttctactagt tctggtttca aacgaggtgg
     1261 taaaacagca agccccatta cttcgatcaa cccaatgttc tcctttttga tatgttggac
     1321 atctttatgt ggatgaaaaa tcccatcagg atgttcttct gaaacattgt tgtctcgaag
     1381 gacaaggtcc aactcaaagt aattgtccct tcttcgtgca attggcgtaa tcgtatggtg
     1441 tggtgtacca tctgctgaaa aagctctgat agatactgtt tcatccgaat atgctttcca
     1501 cttttccaat actgagttag ctgcttcaat caacaatttt ttctctgttg attgcaaacg
     1561 gatcacagac atcggccatt tcacgactcc tgcgatgact tgcggataat ctgctagttt
     1621 gataagtttt tctatcggtg cttttgccat aggaaattca tgacgtcctg cctgataatg
     1681 atcatgagaa agaatcgatc ctccaacaat cggcaagtca gcattcgaac caacaaagta
     1741 atgaggcaag acttcagtaa tccgaagcag tctagcaaat gtttcctttg tgattttcat
     1801 cgggcggtgt tgttctgata aaatgatcga atgttcattg taataagcat aaggagaata
     1861 ttggaatccc cagctttcac cgtctagatt cattcgaata atccggtgat tggtgcgggc
     1921 tggatagttc aagcgacctc tatatccttc gttttccatg caaagcatgc aggtaggata
     1981 gtctactttt accgcctcgc gttctgctgc gatttgtttt ggatcttttt ctggcttaga
     2041 taaattgatt gtgatttcca gttcaccata ctccgactct gccggaaatt tgatgttttt
     2101 tgcgatggct cttgttttga tgtagtcatt tttcttactt aaattaaaga aatactcagt
     2161 tgcttcctct gggcttttcg aatagtgctg tgcaaagaat gcattgacga ctgaaggtgg
     2221 cggtgtgaga aaatccatta attgcgcttc aaaaatctct tgttctgctg gcaattctga
     2281 aagaacacca ttttctcttg ccttagcgac aagctgatcc aataagtcta cagattcttc
     2341 cttaactggt cttacttcaa tgctttctag agcatcttct cctaccatcc ctaaaagacg
     2401 attctgtaaa tataagcggt ccatttccat ccatccgcca gcttcgatcg caagtgtggc
     2461 aaaatcacaa atcgtttgac tgattgacat atctcattcc cctattcttc ataaccattt
     2521 ggatggctga catgccaatc ccatgctgtt ttgataattg ctttgatatc tgtgtattca
     2581 ggtttccaac ccagaacctc tctcgctttt tcacttgaag cgactaatcg gctaggatct
     2641 ccagctcttc ttggtgctat ttctgctgga atttcttttc ctgtcacttc tctagctgct
     2701 tctagcattt ccttgactga gtatcctttg ctgctgccta aattgaagaa gttgctttca
     2761 tttccttctt tcaagtattc caaagctaaa atatgtgcag caatcaaatc ttctacttga
     2821 acataatctc tgatacatgt tccatctggt gtatcataat cgtctccata aacagccaat
     2881 gcttttcttt gaccaagagc tacttgtaaa atgattggga caagatgcgt ttctggtgta
     2941 tgatcttctc cgatagatgc atctgctttt gctccggcta cattgaagta gcgcaaagcg
     3001 acatagcgca tgccatatgc ttggtcacac cacttcatca ttttttccat catcaattta
     3061 ctttcaccat acggattttt aggattagct ggtgtatctt ctgtgattgg agactctttt
     3121 ggttctccgt aagttgctgc agtagaagag aacacgattt tattcacatt gaactcgtgc
     3181 atgacttcaa gtaagatttg cataccgtat acgttgttgt tgaaatacat caatggtttt
     3241 tcgacagatt ctcctactaa agagcttgca gcaaaatgga ttactccttc aatcggttcc
     3301 ttttcaaata cggaacgtag aaattcttta tcccggatgt ctccttcata aaaatgagca
     3361 tctggatgta ctgcttgttt gtgacctgtg agcaaattat ctaccacagc tacttgatac
     3421 cccttttgaa ctagctggtc tacagcgtgc gaaccgatat aaccggctcc gcctagtact
     3481 aaaattgtca taaaaaacac tcctttgatt agtaaaattg tgtttgttga ttatatataa
     3541 tttaaaaaaa cgaagtttac ttttat
//
LOCUS       DASJTC010000158         3525 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.164, whole genome shotgun sequence.
ACCESSION   DASJTC010000158 DASJTC010000000
VERSION     DASJTC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.164"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..435
                     /locus_tag="U4B19_002712"
     CDS             <1..435
                     /locus_tag="U4B19_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEN2078370.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="U4B19_002713"
     tRNA            complement(759..842)
                     /locus_tag="U4B19_002713"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="U4B19_002714"
     CDS             1370..1870
                     /locus_tag="U4B19_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2078371.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="U4B19_002715"
     CDS             complement(2060..2404)
                     /locus_tag="U4B19_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEN2078372.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="U4B19_002716"
     CDS             complement(2558..3478)
                     /locus_tag="U4B19_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEN2078373.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
BASE COUNT         1098 a          687 c          582 g         1158 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgta
//
LOCUS       DASJTC010000159         3479 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.165, whole genome shotgun sequence.
ACCESSION   DASJTC010000159 DASJTC010000000
VERSION     DASJTC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.165"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            146..379
                     /locus_tag="U4B19_002717"
     CDS             146..379
                     /locus_tag="U4B19_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEN2078374.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            376..783
                     /locus_tag="U4B19_002718"
     CDS             376..783
                     /locus_tag="U4B19_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEN2078375.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(1920..2438)
                     /locus_tag="U4B19_002719"
     CDS             complement(1920..2438)
                     /locus_tag="U4B19_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEN2078376.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1181 a          590 c          523 g         1185 t
ORIGIN      
        1 agcaatctaa attttttgaa ttttcttaat taattatata gaaagaacaa tctttctata
       61 gagttagaca tgagagggac tatgcttgat ttaaaatcaa caagcgtata tactgtatat
      121 acaaagaaaa aggagggaga agactatggc aattaaagaa agaaaattta gggaagtggg
      181 gaactcagta gtattaacta tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg
      241 agatacggtt tttttagatg aagaaaaact tcgagcggca attacaaaaa aacaagcctt
      301 atctgatgaa attgatttag caatatctca agcattaact gaatatgata ctacttttag
      361 agagcttgtc gataaatgag tattcattac ctagaagcca aagatattat aaaaatgaat
      421 gtaatccaaa taaaaaaata ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct
      481 ttagaaatgt gtgtagggca acttcgagcc tcagcttttg gtgaagaggt ttatccttcg
      541 atttatgaaa aagcagctat cctacttata caattgataa agaaacatcc ttttcacaat
      601 gcaaataaac gtactgcttt cttagctact tttgtctttt tgaaaatcaa tggacagctt
      661 ttagctattg atcaaaaaga tgctgtaaat ttagcggtat atattgctac gtatgataag
      721 gatttcgatc aactaaaata tgaagtgatc aacactataa agacacatac gcagcccatt
      781 taaggagtta ctgtatgtgt ctttataggg ttattagctg ctatagcgaa ataattgaag
      841 taaattacaa atagctataa ttcaaatcca actatataga aaacgtaaat tttctatata
      901 gttggattaa gataacgact atttatttta tcatattttt actataccct atacttttac
      961 ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt aagttagatt
     1021 aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt ataaattgat
     1081 ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt
     1141 gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga
     1201 aagacctttt acatcttgca gaaatcgtta ctgggattaa actaagcaat aaaaactaat
     1261 gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt
     1321 caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa
     1381 taggataggc gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta
     1441 tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt
     1501 gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga
     1561 cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact
     1621 atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag
     1681 tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc
     1741 tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag
     1801 tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt
     1861 tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt
     1921 tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact
     1981 gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc
     2041 cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta
     2101 atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag
     2161 gtatttgtaa accacttata taactctcct aactgattcc atttaaattg atcagaaggc
     2221 gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa
     2281 ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac tcttttatcc
     2341 ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt cctatttatt
     2401 tcttttttta attcttcttt atttttatat gttctcatta tattacctcc ataaattcct
     2461 aattgtctcc tttaattcat ctataatttc atccacgata ttttaatccg ttctacaatg
     2521 catatctgat atgcattgac tgtctaactc atatgtgaat gatttataaa atatcccaac
     2581 gtctcatcta aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa tatatttctt
     2641 ttgtagtaaa atcggactgg tttgcatatc attttcttcg taaatatcaa aatcatgtta
     2701 actactatca attactaata aaaactacca ctttaagcgt taagaactgt taagtttagt
     2761 taagtgagga tagttcacaa aatccattgt taaacttcac gaaagtggta aaactcaaaa
     2821 tgctttatat aggattacag catcgctctt tcttcgattg cgcgttgggt aaaaaatatt
     2881 atgaagtaaa aattgacgat gacacgttcc ttacggctca tcagatgaaa cagctacaaa
     2941 aacgaaatga tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc
     3001 tcgctcgacg aactattaaa ggtcaagatt tacctgaaga tcatcctttt taggaaattg
     3061 aagcttttta tgttgcacaa tgcatttata atactacaat gatgttttca ccagatattc
     3121 ttctagggtg cggtgtaatg aaacagatac acttgcaaaa gaaaattcac cgcgaattta
     3181 ctaaaattat gaataattat gttaatcttc cgaaattttt ttccaaaatt aagggatcaa
     3241 gctggtgtta tagattgttt aaggttggca aaaaaataac ttctttttaa acaattatag
     3301 aaaaccgata ctctgttaag ttggttgaca cggtgtcgat ttttatgact ttcttattct
     3361 gaaacttcac taacggacag tacctaaaaa gtctatagta tggacgttta cgagtactat
     3421 tttatcaaaa atgctgctca tttcaactta tatgttttag tgaactaccc ccacttagc
//
LOCUS       DASJTC010000160         3325 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.166, whole genome shotgun sequence.
ACCESSION   DASJTC010000160 DASJTC010000000
VERSION     DASJTC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.166"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..2232)
                     /locus_tag="U4B19_002720"
     CDS             complement(<1..2232)
                     /locus_tag="U4B19_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078377.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="U4B19_002721"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="U4B19_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          912 a          744 c          536 g         1133 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaatt
//
LOCUS       DASJTC010000161         3299 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.167, whole genome shotgun sequence.
ACCESSION   DASJTC010000161 DASJTC010000000
VERSION     DASJTC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.167"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..697
                     /locus_tag="U4B19_002722"
     CDS             <1..697
                     /locus_tag="U4B19_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEN2078378.1"
                     /translation="KNTKVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSV
                     KYYDEIANSLTEALYSTIRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMF
                     LRVGVQAKAVLDPHFQAQVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAI
                     TNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELR
                     EKVLRSIIDKSIE"
     gene            complement(1380..1478)
                     /locus_tag="U4B19_002723"
                     /pseudo
     CDS             complement(1380..1478)
                     /locus_tag="U4B19_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1743..2198)
                     /gene="tnpA"
                     /locus_tag="U4B19_002724"
     CDS             complement(1743..2198)
                     /gene="tnpA"
                     /locus_tag="U4B19_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEN2078379.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            2441..3299
                     /locus_tag="U4B19_002725"
                     /pseudo
     CDS             2441..3299
                     /locus_tag="U4B19_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1087 a          576 c          576 g         1060 t
ORIGIN      
        1 gaaaaacaca aaagttggcg atgctactat tattcgcttt tgtcaaaaaa ttggtttttc
       61 tggtttttct gatttaaaaa ttgaaattgc aaaggaagac tttagtcaaa aaaaagaaca
      121 tccgactagt gtaaagtact atgatgaaat tgctaatagt ttaacagaag ctctctattc
      181 cactattagg cttttagacg aagaaaaatt gaatgaagcc attaaaatga tcactcaatc
      241 aaaaaacata tatatatttg gagtgggatc tagtggaaat actagtcttg atttagaaaa
      301 tatgtttctt cgcgtaggag ttcaagcaaa agctgtacta gaccctcatt ttcaagccca
      361 agtcgcttct ttattaacag ttaatgattt agtaatcatt ttttctttat ctgggaaaac
      421 gaaagatact tatgattcat tgaaaatagc taaaaaaaat ggcgcaaaaa ttattgctat
      481 aactaactat attcattcac ctataggaaa gtctgcagac ttagttttac aaacagccat
      541 tgaagaattt cttaatggtg gatctttagc tggaaaaata tcacaacttt atatttgtga
      601 tctattagtt catggttatg aacaaaacaa taatattgat tcagttgaat taagagaaaa
      661 agttttgcgc tctattattg ataaaagtat tgagtgaact acccccactt agctaaacct
      721 aacggttttt aacgattgaa gtaggcaatt cttgggatga gagcatactt gatagcttat
      781 tttttactaa tagcggtggc tcttattgac caaatatccc tgtagttcct acggttcttg
      841 attttatcta aagatatttt tatcgtggaa gctattgatc aacttgaaag aaaaacgtgt
      901 agctcattat cactagttta tcgagtatgg atgaagcaat ggatcatcct ctcttaaata
      961 aatttattaa ggatctgatc cttcaaattt tttccatgat cgatgagcag gaacctactg
     1021 aatctaaacg acatcgggca caaggaatta ttaagattgc taagcgaatg ttttctataa
     1081 agggtgttct aagctataca gtgttaattc aaaagattcc caatcatctt gtttataaga
     1141 acaacgttga agattaaaaa agtgtttcaa tgctaaaatt gcaaaatact tcaatgttat
     1201 gagacaaact gtctataaga atcaatttcc acccatttca tcacttgtta aaagtttatg
     1261 atccttttga tttttatatt aatttatctg tatttaatac agattttatt tattagttca
     1321 tctgcctatt gtattttatt ttgttgcctt accatctgaa gtaaatatac tcttaattgt
     1381 caaattaagt tagacagatc atcaatactt acgtaactca aaaatacttc caaaggtgtt
     1441 cgataatcca gtgattttct aggaagatta tttctgcgcc agcattttct aatgtttgaa
     1501 tttgtgtttt gtaatcttgg ttgcttgtgc taattcttga atagccgtaa attgccaaaa
     1561 tttcatcacc ctttctggac ataagtttct gacaccagtt aaaatcaagt ctatcagtaa
     1621 cccatacacc cgtgaggacg ggtgtatttt taatccccct agaaggggtc gacactcgct
     1681 gccccttaaa aggggatttt taaaagaccg ccaactgctc atcttcactc acttacttct
     1741 ctttattttt taaatggttc tacgtactct cttttactta taatatatcg aatgcgatca
     1801 tcagcttctt gttcacggat gtatttttca tttgtctttt gattcaatcc gactgtactc
     1861 acatagtatc tcttcgacca aaaggatttg tttccgtatt tatatttgag atttgcgtgc
     1921 tgctcgtgga ttagtaccgc actacgacct ttcagatacc ccataaaact ggatatacat
     1981 atttttggtg gtactcgaac aagcatgtga atatgatcta gcattgcatg tgcttctatg
     2041 atttccacat ctttcatttc gcacaacttc ctaagtattt ttccaagatc ttttcgtaat
     2101 tgcccataga ttacttttcg ttgatatttt ggtgtaaaaa taatatgata cttacaattc
     2161 caacgagtat atgctaaact tttatcgtca tttgacatga aaaaactcct ttcgatctga
     2221 tgagcggttg gtagccactc actagtatat cgaaaggagt ttttatcgct aaagatactt
     2281 cttccacgcg tagaaacgcg cggttttctt gtttcgcacg ctttacgcac aaaactgact
     2341 gaagtcatta agaaaataaa agggtctaaa acttttaagt tttgtaccga cccccaaaag
     2401 ttagaccaaa aaatctaact tttggaggtc ggtatttttt atggctaagt atacttttga
     2461 acttaaatta aagatcgttc atgattactt ggatggtaaa ggcggaagtg attatctggc
     2521 aaagaaatat tccattaagg ctccatcaca agtgaagcgc tggatcaatg cctatcaaga
     2581 atttggtgaa gagggtctag taagaaagcg acaaaagaaa aaatattctg ttcaatttaa
     2641 gctggatgcg atagagttat atctaacaag tgagctgtcc tatcgtgaag ttgccaacac
     2701 acttaacatg aacaacccta atttgatcgc tagttggatg cgtcaattcc gtgagggtgg
     2761 gatcgatgga ctctcaaaaa cgaaaggatg tccacctatc ttgtctaaga aaaatgaacc
     2821 caaaaataag aaaaaatcta caactaaagc aacgtctaaa gaacgtgaac gcatcgagga
     2881 gttggagaaa cgagtccgtt cccttcaaat tgaaaatgcc tttttaaaag aattgaggaa
     2941 gctgcgaaaa caggaagccc aacaacgacg aatgaagcaa tcgcacgaat catcgcaagc
     3001 ctccgaggac cgtttaaatt agttgagctt ctagaaactt taaagttccc taaggcaatc
     3061 tttatgtact ggcaaaaacg attggaccgt aaaaatccag atcaagagat tgaagcggaa
     3121 atgcttaaag ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta
     3181 agaaaccgcg gtttctctat caataaaaag aaagttcagc gcctgatcaa gaagctagga
     3241 ataaaagtaa cgacatacac acgtaaatca cggcgctaca attcttatcg aggacaagt
//
LOCUS       DASJTC010000162         3285 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.168, whole genome shotgun sequence.
ACCESSION   DASJTC010000162 DASJTC010000000
VERSION     DASJTC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.168"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..508)
                     /locus_tag="U4B19_002726"
     CDS             complement(<1..508)
                     /locus_tag="U4B19_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2078380.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEM"
     gene            complement(480..1679)
                     /locus_tag="U4B19_002727"
     CDS             complement(480..1679)
                     /locus_tag="U4B19_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN2078381.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1809..3185)
                     /locus_tag="U4B19_002728"
     CDS             complement(1809..3185)
                     /locus_tag="U4B19_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2078382.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3185..>3285)
                     /locus_tag="U4B19_002729"
     CDS             complement(3185..>3285)
                     /locus_tag="U4B19_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2078383.1"
                     /translation="THSKEVADSADIILELSGKKLKKVNKMNLEVE"
BASE COUNT         1011 a          629 c          492 g         1153 t
ORIGIN      
        1 tcatttcttt cgtaaactta tgtttattgg cgttagcttt gatgtgggta gaatccagat
       61 acaaattatc ctctgttaca aaacctgctt taactgcttg ttctaaaata taggcaaaaa
      121 tatcctcaaa caccgtcgtt tctctaaatc ggcgaacata attctttcca aaagttgaga
      181 aatgggggat tttctcctcg aaggagtagc ctaagaacca gcggtaagcg acattggtat
      241 cgacttcctt aatggtttgt cgcatggaac ggataccgaa taaatactga ataaacacca
      301 atttgatcag aatcactgga tcgatacttg ggcgacctaa atcggagtaa gtggcttcga
      361 cgattggata aataaattca aaatcaagcg cggcatcaat ctttcgaacg agatgatctt
      421 gaggaactag atcatcaatc gtaagcatcg caaattgatt tctaccattc gtattgtttt
      481 tagacatcat agctatcact ccttgcattc gttttcataa atctattata aaagaaaaga
      541 ctgtagacat catccataaa atggactttg tctacagtct gcgcattctc attagagaac
      601 actccctttt ttgtgacttt tctgaagcta gagtgaatac tttcaactgc attagtagtg
      661 tacatgattt ttctaatggc gctgccataa tcaaagagtt gttcaacatg agaaaagttt
      721 cgtttgcata catctactgc accagaataa tgagaccacc gattttgaaa actgtcgaag
      781 gcagcgtgtg cagcgtttaa agaagaagca ctgtagaatt ttttcatgtc tcgacagact
      841 tccttataat ccttacttgg gaggtatcgt aacgcatttc gaacaagatg aacaatacag
      901 cgctgcacaa tgacagaagg aaaaattgct tttgcgcctt ctttgagacc ggaaacaccg
      961 tccatcgaaa tgaaaaaaac atcttctaca ccacgtactt tcagttcatc aaatacttgc
     1021 atccagcgat ttttagattc tgtttgattg agccataggc ctaaaatctc cttgtttcct
     1081 ttaagatcat agccaagaat ggtgtacaca gcatattctt tagcttcata gttttcgcgt
     1141 aaagtgacgt acatacaatc aacgaataga aatgcataac acttggctaa tggccgagct
     1201 tgccattctt ccaattcagg aaggacggca tccgtgatat cagaaatcat ttcgtgggaa
     1261 atatcaaagc cgtaaatatc ttcaacggtt gcggcgatat cgcattgact cattcctcgt
     1321 gcatacatgg aaagaacctt accttcgatg tcggagacat ctcgttttct attaggaatc
     1381 aactcaggtt caaaagaagc ctcccggtct ctgggaacat caatgtttac tttaccaaag
     1441 ctcgttttaa gcgtcttatt gccataaccg tttcgtcgat tgtcatgttt ctttggctct
     1501 ttagaatggg cgtcataccc taaatggtta ttcaattctc cttgaagcat tttttcaaat
     1561 aagggaccaa acacatcttt taaaacatct tgcatatcct ctacagattc gggttgataa
     1621 gtattcaaaa tggattcagc taacttctca gcatcaggat ttcttttctt tctagccatc
     1681 gtgtaatcac ctcttgatct tattttagaa aaataaaaac cgtgaagcaa ccacccgcct
     1741 aggattgatg gttacttaca cggtttatat tacactctca tttttggtgc taaaaagcac
     1801 cgaaaatttt aattattgtc aattaataat tcttttggat tccttcttaa tatattgaat
     1861 gaagaaataa tcaatgatac aagaagtact aaactcataa atataactac ataaatcatg
     1921 aatttaggta atacattgat atctaaactt gataatgtct tgttaaatcc ttcagcttct
     1981 gccccaccac ctaattggct agatgcagat tgtttagcta tttgtttagc tatatcacca
     2041 gtaaccttat tcaatatatt attcccaagc ttgtcagctg tatattgagc taagaaatag
     2101 gaaccaacga atgcggggat tgatataaat accatctcaa ttaggaattg accaaaaatt
     2161 tctaattttg atatacctaa tgatagtaat actgctattt ctttctttct agcattcatc
     2221 cataagaata ataataatga aactacaact cctgcaaata ttaatgagcc aaaaaataat
     2281 ttatttgaaa ttgagtaaat acctgatata gattgttgta atgcagggta atttgatgaa
     2341 cttttaatca aattatattc tctccaattt atatctaatt tcccaagatc ttttattaca
     2401 ctatcaagat tcttatcacc ttttacaaag aatgttgcat cttggtatac agctgtatct
     2461 tcggtattac cataaacttt agcagctgta tgaatatcag tgattaatgt attttcgtat
     2521 agttcttgtg ctgcacttac tccaccgcta ttatgcccat cgaatagacc tttaatttct
     2581 acttctactg tttcatctgc acctttctca ttatcagcat caaataaatt agattttatt
     2641 tttatcttat ctccaacttt aaggttattt ttttcagcta aatctttatg cattaagatt
     2701 ttatttttat cttcattttc taaatgcttt ccttctacta atttatatgc ttctgataca
     2761 aattttgttt cttttgttga gtcgttaacc ccagttaaca taactgttct tttaaaattc
     2821 tttgctcttt caggtgattg attcgcaaga gtctcttgag tttcaataat atcataatca
     2881 actaaatctg caacactgtt tattcttttt acataggagt ctatactatc tgtttgagat
     2941 atctttttaa tatcttcacc ttttacattt cccccacctc taggtgttcc tggatttact
     3001 tgtctattta tctccataga aaaactattt gttatattag caaatgtttc ttttgaagct
     3061 ctgtccgtag catctttaat agataaacta atgagactta aagttgacat agataaaata
     3121 actaatatga taattaatga ttttaaactt tttctagtta cataagcaaa tgcatttttt
     3181 atcattattc aacctccaaa ttcattttat ttactttttt caacttttta ccacttagtt
     3241 ctaaaatgat atccgcagaa tctgctactt ccttactatg tgtta
//
LOCUS       DASJTC010000163         3228 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.169, whole genome shotgun sequence.
ACCESSION   DASJTC010000163 DASJTC010000000
VERSION     DASJTC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.169"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            130..1794
                     /gene="alsS"
                     /locus_tag="U4B19_002730"
     CDS             130..1794
                     /gene="alsS"
                     /locus_tag="U4B19_002730"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEN2078384.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            1807..2517
                     /gene="budA"
                     /locus_tag="U4B19_002731"
     CDS             1807..2517
                     /gene="budA"
                     /locus_tag="U4B19_002731"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEN2078385.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            2647..3144
                     /locus_tag="U4B19_002732"
     CDS             2647..3144
                     /locus_tag="U4B19_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEN2078386.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
BASE COUNT         1093 a          530 c          716 g          889 t
ORIGIN      
        1 acatatggct aaacaaatga aataaaacat gctatacttt tgaatgaaac aggtttagaa
       61 actgtatcac ttatttgtga ttatttctaa ctaataatta aaaataaagc ataaaaagga
      121 cgatgttcaa tggctgaaaa aatgaaacaa ggttcgaaat ctgtagtaga aagtttgatt
      181 aatcatcatg ttgattatgt attcggtata ccaggtgcaa aaatcgatgg tgtatttaat
      241 gaattagagg atcaaggtcc tgaattaatc gtgactcgtc acgaacaaaa tgctgcattt
      301 atggcacaag caatcggacg tattacagga gagccaggag tagttatcgc aacaagtggt
      361 cctggtgcta gtaacttagc tacaggtttg gtaacagcga cagcagaagg tgacccagtt
      421 ttagcaattg ccggacaagt aaaacgaagc gatctattga agttgaccca tcaaagcatg
      481 gataatgcag cactgttcca gccaatcaca aaatacagtg cagaaatcca agatccagaa
      541 acaatttcag aagtgattgc caatgcttat cgtatggcta agagttcaaa aaaaggcgcg
      601 agctttatca gtatcccgca agatgtagtg gatgcacctg tccaaggtaa tgtcatcaag
      661 ccattgagcg atcctaaatt aggttctgct tctgcagatg atatccgtta cctagctgaa
      721 cgcattagag aagcaaaact accggtacta ttagtaggta tgcgaggatc aagtgaaaaa
      781 gaaacgttag ccatcaggca attagttgaa aagacagcat tgcctgttgt agagacattc
      841 caagcagctg gagtgatctc acgtgaatta gaagcgcatt tctttggtcg tgtgggttta
      901 tttagaaacc aaccaggaga tatgcttttg aaacgaagtg atctagttat tgcaattggc
      961 tatgatccaa tcgaatatga agcaagaaac tggaatgcgg aaaaagatgc gcggatcatt
     1021 gtgatcgatg aagcgccagc tgaaatcgat ccttttatgc agcctgaaag agaattgatc
     1081 ggtgatatct cagccactct tgatttattg acaggttcat tagagcctca acaagtgtca
     1141 gaagatgcaa aagaatattt agcatctctt caagcaaaat taacggagcg ggacattgtt
     1201 caatcaaaag gagaagcagg tatcttgcat cctttagaag ttattaacac gcttcagtca
     1261 aaagtaacag atgatatgac tgttaccgta gatgtaggta gtcattatat ttggatggca
     1321 cgccacttta gaagttatga acctcgtcat ttgttattca gtaatggtat gcaaacgctt
     1381 ggtgttgcat tgccatgggc gatttctgct gcattggtgc gtccaaatac acagatcgta
     1441 tcagtttccg gcgatggggg atttttattc tctgcccagg atttagaaac ggcggtacgt
     1501 aaaaaattga atatcgttca tttgatttgg aatgatggac actacaatat ggtagagttc
     1561 caagaaaaaa tgaaatatca gcgggcgtca ggtgtagact ttggtccagt tgattttgtg
     1621 aaatatgcag aagcttttgg cgctaaagga atccgagcta caagtgtaga ggaattagaa
     1681 aaagctttag aagaaggatt tgcgacagaa ggacccgtaa ttattgatat tccgattgat
     1741 taccgtgata atgagaaatt gggagaaaca atcttaccag atcaattcta ttaaggaagt
     1801 gtcgacatga ctgaaaaaat cttataccag cacgggacat taggagcttt gatggccggc
     1861 ttgatggatg gaacagaaac gattgctcat atattagaaa aaggaactct gggattggga
     1921 acattgcatg ggcttgatgg tgaagtcatc tttttagatg gcgtagctta tcaaggaaga
     1981 tcagatggct cagttgccca attagatgga agtgaactta ccccctatgc agcaattaca
     2041 gattttacgc cagatactag tttttctgtc tcaaaaactg cagatggaga acaattgaaa
     2101 aaagacatat tagtaagaga agccggggaa aacctatttt tggcagtgaa gataactgga
     2161 ttattcaaaa atatgcacat ccgtatcatg ccgaagcaag aaaaacctta cagaagacta
     2221 gcaaaaattt cagagtctca accagaattc caacaaagca atgtccaagg aacattagtc
     2281 gggttcttta cgcctgaatt attccaagga gttgctgcag cgggatttca cctgcatttt
     2341 atcgatgata cacatacatt tggcggacat gtaatggatt ttgaagtggc tgaaggaaaa
     2401 gttgagatta gccgcatctc gtcccttgta cagcattttc cagttgatga tccgactttt
     2461 gttcacgaaa cgattgatta tgctgattta gcagcagaaa tccaacaagc agaatagaaa
     2521 aagacagcga gatgcgttca aggtgattaa cctgacgtat ctcgtttttt tgaggagaaa
     2581 acctagcgtt tacaaaaaaa ctgagctata atcttctcca tagatcaaaa aggagcggtt
     2641 tttatcatgt ggacagaaaa acagaaaaaa gatgcatacg aaattttatt attacagcaa
     2701 caaggattat tagaagcaga agataactgg ttagcaaatt tagcaaattc atctgcattg
     2761 ttgaatgaga ctttgcctga gactgtcttt gcaggatatt atttattcga tggtaatgaa
     2821 ttgattttag gaccgttcca agggcgagtt tcttgcacac gtatcaagat gggaaaagga
     2881 gtttgtggag aatcagctga aaaacaagaa acattgatcg ttgatgatgt caaaacccat
     2941 gcaaattaca tttcctgcga ttcagccgca caatcagaaa tcgtagtgcc aatggtaaaa
     3001 gaaggtaatt taatcggtgt tttagatatc gattgcggag taacgaaagg gtatgatgaa
     3061 atcgatcaag catttctaga aaaatatgta gatcttttat tagcaaattt taatataaat
     3121 gctttttcat ttattaataa ctaaaatcgt ctattgaaac aaaaaagaag agaaatcaag
     3181 aataaaaaga gccggaaaga cagtgggcat ttctgttttt cggttttt
//
LOCUS       DASJTC010000164         3166 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.170, whole genome shotgun sequence.
ACCESSION   DASJTC010000164 DASJTC010000000
VERSION     DASJTC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.170"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(218..586)
                     /locus_tag="U4B19_002733"
     CDS             complement(218..586)
                     /locus_tag="U4B19_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEN2078387.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            736..1218
                     /locus_tag="U4B19_002734"
     CDS             736..1218
                     /locus_tag="U4B19_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEN2078388.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1255..2265
                     /locus_tag="U4B19_002735"
     CDS             1255..2265
                     /locus_tag="U4B19_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEN2078389.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2380..3093)
                     /locus_tag="U4B19_002736"
     CDS             complement(2380..3093)
                     /locus_tag="U4B19_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078390.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         1073 a          512 c          618 g          963 t
ORIGIN      
        1 caggaaaatt gctgaaaaaa taattttact aagtggaaaa ttcaaagcca ttcaatcagt
       61 gatttttttc agcacaagaa agactcctta taaaaatagc agatactttg tcaattaagt
      121 gtttaaccag ggatgataaa aaggatagga ttgaacaaaa cctgtataag ctgatgaata
      181 gtttcctcgc cagtaaaagg tttattcgta taaagcttta tcgccaaaat ttatcatgac
      241 cttttttatc ttcaatatta aatgaaatca attgttcaat caattcccag ttgattacac
      301 ttttccaagg aactttgatg acgttatccg tatatacgta gcctgcttct tggatatttt
      361 tttcaagggc acgaatcgct gctttttccg gtgcgataga aaaatgattt ttagcaaaac
      421 taaatgcaat aataaaggta ccatggtcag taaacatagg ttggttccat tttacagttg
      481 tgtctagctg gggatacgtt tctgttatct tagcaaaaag gtctttgatc ttttgttgat
      541 gttctttttc tttgatctga ttgatgatcg gctgaaattt ttccattcta aaacgcctcc
      601 attaagtggt ttgatttatt ttaccagaaa aacaatagtt atgtataaaa aacgaacaaa
      661 acacaagtaa tgttgtaacc gctatattta tatgataaga tgtctcaaat aataaaatag
      721 aaaagagaca taaaaatgat tgacttggca aagaaattct tacacgaaaa tatatatttt
      781 gagttgtatc atcatcgagc catttataca aatgaagatg ctttggtaat aaagaaagaa
      841 caaggattta caggtacaga gaccaaaagc ttattcttaa aagataaaaa agataataac
      901 tacgtatatc tgacttttac tacgaaacaa acggatttca aagcattagg gaagatcgtc
      961 ggtaaaaggc tttctattgt ttcatcagaa cgcatggaag aacaaacggg acaaaagcag
     1021 ggcgctgttt ctccgtttgg ttatgaaaaa cagattccga ttattattga tgaagagctc
     1081 ttatcaaatg aaaaacttgt ttttgcacca ggtagaccag atcaaacgat ggtcatccaa
     1141 acggttgact taaacaaaat catccagcta cttgagatgc cgatattttt gatgccggca
     1201 aacgaaaaaa acaattaacc aagtaaaata gtgcatactt ggaggtgaag caacatgata
     1261 agttatccat tattagaaag aaatgcaacg attggggtta ctgcaccatc ctcaggcgta
     1321 tcagaggaac accattcgtt actcgaatca gctttaaaga gaatggaaga aaaatctttc
     1381 caagtagtta ctggaaagac gctttggacg caagataagg aaaaatcagg tccagcaaga
     1441 aaacgagcag atgaattcaa tcaaatgatg caagatgaaa atatccaaat gatctttccg
     1501 ccttggggag gagaattatt gattgagatt ctcgattttg tcgattttga tgctctacaa
     1561 ccaaaatgga tagtaggtta ttcagatatt agtacgttgc tacttgctgt cacattaaaa
     1621 acagggatag ccactgcaca tggaaccaac cttattgatt taagaggaga gtacttggat
     1681 gaaacgacag caatgtggga aagtgtactg aaaacaagag aaggagaatc gattctacag
     1741 caaccttctg aaaagtatca aaaagagtgg ccccatgagc attcatcatc ttctgttttc
     1801 catctcactg aaccaacaaa ttggcaatca gtcagcggag atgcagtaag gatgaaggga
     1861 cgattattag gtggctgtat tgatacgatc agtcatttaa tcggtacccc ttatggagat
     1921 gtccaatctt ttaaaagaaa gtataatgat ggaaagccga tcttgtggtt tctagaaaat
     1981 tgtgagctat cgtcagttga tttaagaaga tcacttgttc agatgaaact ggcaggatgg
     2041 tttgatggca gctcggggat catgttcggt agaagtgcag cagaggattt gttagattat
     2101 aaaatgaagg atgtatatga agatctgtca aaggaattaa aactaccgat cttgtatgat
     2161 attgattgtg gtcatgtacc gccacaactg acattgatca atggtgccta tgcagaagta
     2221 gcggtagaag atagaaaagc gactgttttg caaacgttta tttaaaaaat tttttcctgc
     2281 cttggagttt atcaaagacc aacaaatcta aatagaaaag aaaatgaaag aagctgacag
     2341 tattttgcca gcttcttttt gtatgatcta ttagaaattt taattatcca taactatcac
     2401 attgatcagc tgtttcacat catttggatt acctgtagga atagctacta cagatgcatt
     2461 atcaataatt tcttgagttg tttctctaat gaacgctgga taagatgttt gatctgcagg
     2521 catttgccaa tgtgaaggaa ctggataact agtgattgtc ccgtcatggt tacttgaata
     2581 cactaaaact ccctggtaac tgtatttccc agtgagcata accacatctt tcggatagac
     2641 tgcactccct gcatcataag gggcgatagg tgttcctgca ggataatgaa taacatgtag
     2701 ttcgaaatca cccgatgtat ctctaaaagt attacctcta attgctagcc aaacacgtgc
     2761 gtactcaatt tgtagatcag aatatcctgc taacggatct attgactgag aagactgtga
     2821 ggaagttgtg tctattgttg aagaagaaat atttgtctca actgtactac tagatgtaga
     2881 agaagtattg ggctctgaga ctttactgct tgttgaagat gtattcgttt cagtagaagc
     2941 tgatttctga gtacttgatt tttcagctga actggtttgt ttgattgaac ttttgtctgt
     3001 ggtcgcagat ggatcactat tttctttact aggagacgta catcctacaa ggaataaaga
     3061 gaccaacaat aaaataaaac ctaacttttt catataaaat acctcattat ttttttaata
     3121 ccagtatagc ataatcctga atttttgaat ccgtaaaacc ttgtct
//
LOCUS       DASJTC010000165         3162 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.171, whole genome shotgun sequence.
ACCESSION   DASJTC010000165 DASJTC010000000
VERSION     DASJTC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.171"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(63..2555)
                     /locus_tag="U4B19_002737"
     CDS             complement(63..2555)
                     /locus_tag="U4B19_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEN2078391.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="U4B19_002738"
     CDS             complement(2562..3029)
                     /locus_tag="U4B19_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEN2078392.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT          838 a          697 c          518 g         1109 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tc
//
LOCUS       DASJTC010000166         3159 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.172, whole genome shotgun sequence.
ACCESSION   DASJTC010000166 DASJTC010000000
VERSION     DASJTC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.172"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            19..1026
                     /locus_tag="U4B19_002739"
     CDS             19..1026
                     /locus_tag="U4B19_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEN2078393.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1038..2042
                     /gene="neuB"
                     /locus_tag="U4B19_002740"
     CDS             1038..2042
                     /gene="neuB"
                     /locus_tag="U4B19_002740"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEN2078394.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2039..>3159
                     /gene="neuC"
                     /locus_tag="U4B19_002741"
     CDS             2039..>3159
                     /gene="neuC"
                     /locus_tag="U4B19_002741"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEN2078395.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDI"
BASE COUNT         1197 a          406 c          605 g          951 t
ORIGIN      
        1 tcataggagg agatataagt gtttaaagga aaaacaatat tagtaactgg cggaactggt
       61 tccattggaa gtgaaattgt tcgtcagctt ttaaaatatg aaccaaaagc tatcagaatt
      121 tatagtagag gtgaagaaaa acaattttat atgcaacaag agctctcaaa tcacactaat
      181 ttgagatttt taattggtga tatcagagac tctcaaagga ttgattatgc atgtcgtgat
      241 gtggatatta tttttcatgc tgccgcaatg aaacacgttc cagcaagtga atataatcct
      301 atggaagctg taaaaacaaa tgtgatggga actcaaaatg taatagatgc agcgatcaaa
      361 aataatgtaa aacattttgt aggtatcagt acagataaag tagtgagtcc tactaacacc
      421 atgggtgcga caaagttatt gagtgaaaaa ttaataatag ctgccaatca gtataaagga
      481 aaaacagaaa caatattttc ttgtgtaagg tttggaaatg tcatggggtc aagtggatct
      541 gttattcctc tttttagtaa acaaataaaa aacaataaag atattacttt gacagatgtt
      601 gatatgacac gatttatgat gtcaataccg gatgctgcaa gattagttct gtctgctgct
      661 atgaattcaa aatttggaga aacatatgtc ttaaaaatgc cggcaatcaa tattttaact
      721 ttagctcaat cattaataaa aatatataat gatacacatg atgaaaaata tagaggtgaa
      781 ataaaagttg taggtatcag gccaggagaa aaattatatg aagagctaat gactacagac
      841 gaaattgaac gctcatatga aaatgataaa atgtatatca ttccaccaca ctatcatgct
      901 actgacgaac tgtacaaaga ttatgaaaaa attaaaatca ctgcatactc ttcagatgca
      961 acagaattaa tgagtggaca ggaatttatt agaatcgcaa aagagaataa tgtcgtgact
     1021 tggtaaggag ttttatgatg aaatcagaag tatatgtaat tgcagaaatt ggtgtaaacc
     1081 ataatggaag tctagattta gctttagaaa gtattgataa agctaaagaa tgtggagcta
     1141 acgcggttaa atttcaaaca tttaaaacga ataagctagt ttccaaaaag gctccaatgg
     1201 caaaatatca gaaggataat cttaaagagg acagttctca atttgaaatg ctaaaaaaat
     1261 tagaattatc tcaatctgat tttattagaa taaaagatta ttgtatacaa aataaaattg
     1321 attttttgtc tactcctttt gatgaagaaa gtgcagactt tctaaaacaa attggtgtac
     1381 aaggatttaa aattggctca ggagatttaa caaatttacc ttttcttaga aaaatagata
     1441 tgtatgggtt gccagtttta ttatctacag gtatggctga tattcatgag gtcagtgagg
     1501 ctgtagattg tttcatagat agtccagtta ctattttaca ttgtacttca aattatccgg
     1561 ctagttctga agatattaat ttattggcga ttaacacatt aaaagaaatg ttcgggatac
     1621 ccgttgggta ctccgatcat tcattgggat atgatgttgg tatttgtgct gttgcgatgg
     1681 gtgctaaggt aattgagaaa cattttacct tggataagga actacctggt ccagatcata
     1741 aagcttcttt agatcctcag gaatttgcag attttgtaaa gcatattaga aacactgaaa
     1801 tatttttagg tgatggtgtt aagcgagcaa tgccttctga acttagtaca aaagaagtat
     1861 caagaaaaag cctagtggtc actgagaatt taaatgtggg agatattctt acagagaaat
     1921 ctcttacaat aaaacgtcct ggaaatggaa tagctcctaa gtttttatat caatatattg
     1981 gcaaaaaagt taaaagacca ataagtgcag atagtgttct gcttgaggaa gatgttgaat
     2041 gaaaaaaata cttatagtaa gtggaacacg agctgattat ggaatatata aacctgttgc
     2101 tgagaattta ctacaagata attgggatgt atcatttgtt gtttctggaa tgcatttatc
     2161 ccataaatat ggatttacta aagaattgat cttagctgac ggatttagaa ttagtggaga
     2221 agttcaaagt ttgtttttag agaacaataa gggaaacatg gctcggtctg tggcattaga
     2281 gataaatggt ttcacaaata tatttgaaca aactagccct gattttgtat tattactagg
     2341 ggatagagga gaaatgttgg cagctgcgat agcttgtttg tatcttggaa tcaaaacagc
     2401 acatattcac ggaggcgaag tgtctggtac tgtagatgaa tcaataagac atgccataac
     2461 gaaaatagca tctgttcatt ttgctgctac ggaaaaaagc aaagagcgtt taataaaaat
     2521 gggggaagat tcttggagag tttttcccgt cggagctcct agaatagata ctattttaaa
     2581 taatagatta cctagtttta gagatataaa agaaaaatat catctaaaat ttgaaaagaa
     2641 aaaatacgtt ttgcaagtat ttcatccagt tgtaaccgaa ctagaaaata tagaaaaaca
     2701 aattttagct ttgatcaatt caatggaaca acaatctcaa ccaattattt gcattttacc
     2761 taatagtgat gctggtagcg aaattatcag aaaaatgtac cagaaatatt taaaaacgga
     2821 tattatttat attacgaatt tagagccgga ggaatacctg actatattaa aagagtgcaa
     2881 atttatgatc ggaaattctt cttctgggat aattgaagct gcatcattca aaataccagt
     2941 aataaattta ggaaccagac aaaatggacg agaacaatct aaaaatatta taaatgcaaa
     3001 tctaaatgaa aatgaaattg aaactgcttt gaataaaata caagatcctc agtttataaa
     3061 aagcttgaaa aatgttaaga acatctacgg tgacggcaac agttctagaa gaataacaaa
     3121 aacattgaat caaatattga atgatgggga agatattaa
//
LOCUS       DASJTC010000167         3106 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.173, whole genome shotgun sequence.
ACCESSION   DASJTC010000167 DASJTC010000000
VERSION     DASJTC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.173"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            201..1139
                     /locus_tag="U4B19_002742"
     CDS             201..1139
                     /locus_tag="U4B19_002742"
                     /inference="COORDINATES: protein motif:HMM:NF016640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF616 domain-containing protein"
                     /protein_id="HEN2078396.1"
                     /translation="MGRQELYSLAINELEKRNLEIHELNQKNNSILGMKIRNKINQLN
                     QMKKRDNLLPAKYTLKSMTGKGPIKDYSNRENRSDELRKKELTNQGTPKRIAVYSCIT
                     GNYDSIKEPDFSHPLVDYYLYTDNVITSEHWNIREIPKYISELKNNILINRYIKFHPN
                     ELFTDYDFTLYVDGNVTITSDITGLLNSSSKLGLSMHKHSERKSLYSEADALIAVGKG
                     NKESIIEQISKYREEGFPDNFGLAEATVILVDLNNSIAENLLDLWWREYLNSESGRDQ
                     LAFPYVLWKSGHAMKEVTTLGNNVYKSAKLRIISHY"
     gene            1385..2401
                     /locus_tag="U4B19_002743"
     CDS             1385..2401
                     /locus_tag="U4B19_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HEN2078397.1"
                     /translation="MKMKILITGGAGFIGFHLSRKLLKETENQIVAIDNLNDYYDVQL
                     KEDRLTILYDYNNFKFIRMDLLEKEKLDSLFESEGFDIVIHLAAQAGVRYSIENPYAY
                     IDSNIIGFLNILEACRNFPVKHLLYASSSSVYGGNTKVPFSTEDSVDHPVSLYAATKK
                     SNELMAHTYSHLYKIPTTGLRFFTVYGPFGRPDMAYFSFTKNILSGKEIKIFNHGKME
                     RDFTYIDDIVEGITRLINKVPEPNSDWTDIDDLSSESWAPYKIYNIGNNKPVALLEFV
                     ETIEKYLGVKANKIFLDMQPGDVVRTYADIEDLEEKINFKPNITLDEGIKKFVEWYTV
                     YYLS"
     gene            2769..>3106
                     /locus_tag="U4B19_002744"
     CDS             2769..>3106
                     /locus_tag="U4B19_002744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078398.1"
                     /translation="MDSSSNKSHSIAKKAFIYAIGTLSSKMISVIIIPIYAVYISVAD
                     LGSFDYQNTIAQFLSPVIFGAIWEAILRMGLGKEKNIQKKVVTNSICFVIANTVILAI
                     FLPIIYRMIFP"
BASE COUNT         1127 a          398 c          563 g         1018 t
ORIGIN      
        1 agagaacaaa agtaaagaat tatatctact tgcttgcgta tttctttttt atcattgtgt
       61 gtttgatatt catcaatata agaggaactg cttttgtaaa tggaatccta ccattattta
      121 gagaaaatat tatttttaag gctgtttact atttttttga gtaagaataa atagcactta
      181 aaaatagaga tgagggattt atgggaagac aagaattata tagtttagct atcaatgaat
      241 tggaaaaaag aaatcttgaa atacatgaat taaatcaaaa aaataattct attttaggca
      301 tgaaaatacg aaataaaatt aatcaactta accaaatgaa aaaacgagat aatctgttgc
      361 cagcaaaata tacccttaaa tcgatgaccg gtaaggggcc tattaaagac tattcaaaca
      421 gagagaatcg cagtgatgaa ctaagaaaaa aagagttaac taatcaagga acacctaaaa
      481 ggattgctgt gtatagttgt atcactggta attatgattc tattaaagaa ccggattttt
      541 ctcatccatt ggtagattac tatttgtata cagataatgt gattacaagt gagcattgga
      601 atattagaga gatacctaaa tatatatcgg aattaaaaaa taatattttg atcaatagat
      661 atattaaatt tcatcctaat gagctattta ctgactacga ttttactttg tatgttgatg
      721 gtaatgtaac aataacttct gatattaccg gattactgaa cagttcttcc aaacttgggt
      781 taagtatgca taaacatagt gaaagaaaat ctttatattc tgaggcagat gcattaattg
      841 cagttggcaa aggtaataag gaaagtatta tagagcaaat tagtaagtac agagaagaag
      901 gatttcctga taattttgga ttggctgaag ccacggttat tttagttgat ttaaataatt
      961 caatcgctga gaatcttcta gatttgtggt ggagagagta tttgaactca gaaagtgggc
     1021 gagatcagct cgcatttcct tacgtgttgt ggaagtccgg acatgcaatg aaggaagtga
     1081 ctaccttagg caataatgta tataaaagcg ctaaattaag aatcataagt cattattagc
     1141 cctaaatgat ttctaaacta ttctttcaaa tcctagtaaa tatttggaga tagaagattt
     1201 cctttttaaa gtcataaaaa attaagttat attttttgag tgaatggaga agacgcatag
     1261 tatcataaac ttcgttatat cttaaattat ggggtatgcc taaataataa atgtaatacg
     1321 agtataaaga agaaagcaat tttgggttta accctattgg ttcctttcag aaaaaggtgg
     1381 atgaataaaa atgaaaatat taataacagg tggagcagga tttattggtt ttcatttgtc
     1441 tagaaaatta ctcaaggaaa ctgaaaatca aattgtagcg attgataatc tcaatgatta
     1501 ttatgatgtc caattaaaag aagatcgatt aacgatactg tacgattata ataattttaa
     1561 atttatcaga atggatctat tagagaaaga aaaattagat tctctatttg aaagtgaagg
     1621 ttttgacata gtaattcatt tagctgctca agcaggtgtt agatattcta ttgaaaaccc
     1681 ttatgcttat attgattcta atattatcgg ttttcttaat attcttgaag cctgccgaaa
     1741 cttccctgta aagcatctat tatatgcatc atctagttca gtatatggtg ggaacacgaa
     1801 agtaccgttt tcaacagaag attcggtgga tcatccagtt agcctttatg cggctaccaa
     1861 aaaatcgaat gaattaatgg ctcatactta tagccacctt tataaaatac caacaactgg
     1921 gctgagattt ttcactgtct atggtccatt tgggcgacca gatatggcct atttctcttt
     1981 taccaagaat attttatctg gaaaagaaat taagattttt aaccatggaa agatggaaag
     2041 agatttcact tacatcgatg atatcgtaga gggaatcact agattaatca ataaagtacc
     2101 tgaacccaac tccgactgga cagacatcga tgatttgagt agtgaaagtt gggcaccata
     2161 taagatatac aatattggaa ataataagcc ggttgcattg ttggagtttg ttgaaacaat
     2221 tgaaaagtat ttaggtgtta aagcgaacaa gatttttttg gacatgcagc cgggtgatgt
     2281 cgttagaact tatgccgata ttgaagattt agaggaaaaa atcaacttta aaccgaatat
     2341 aaccttggat gagggcatta aaaaattcgt tgaatggtat acagtgtatt atttgagtta
     2401 attttgaaag gaatattgaa gatgggtaat tcatacgatt tttttagctt tgttaggtat
     2461 tttaggaaat tctactttca aacgtgacga actttctcgc cgaatagata ttttctataa
     2521 ctaaaagaat ttctataaaa caaaattgtg tatcaagatg tgtgaactgg attctgactg
     2581 ccaatattga ttgatgagat tgaagaaata tatagttgat catggatatc tttatttgaa
     2641 aagatcttag ctattcacat attcaaaaaa atttttacta cgaggactca cacgatacag
     2701 aggaatttag gtagttctaa ttaaaccaga aagtcatcta tagatgtgag ttgagggagg
     2761 tcatacggtt ggatagtagc agtaataaat cacattcaat agctaaaaaa gcttttattt
     2821 acgcaatagg aacattatca tcaaaaatga tatctgtcat tataatccct atctatgcag
     2881 tatatatttc tgttgccgat ttaggaagct ttgattatca aaatacaatt gctcaatttc
     2941 tttctccagt tatttttgga gcaatatggg aagcaatttt acgcatggga ttaggcaaag
     3001 aaaaaaatat tcagaaaaaa gtagtgacga acagtatttg ttttgttata gcaaatacag
     3061 tgatattagc cattttttta ccaataatat atagaatgat tttccc
//
LOCUS       DASJTC010000168         3098 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.174, whole genome shotgun sequence.
ACCESSION   DASJTC010000168 DASJTC010000000
VERSION     DASJTC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.174"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(18..710)
                     /locus_tag="U4B19_002745"
     CDS             complement(18..710)
                     /locus_tag="U4B19_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2078399.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            705..971
                     /locus_tag="U4B19_002746"
     CDS             705..971
                     /locus_tag="U4B19_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2078400.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1147..1716)
                     /locus_tag="U4B19_002747"
     CDS             complement(1147..1716)
                     /locus_tag="U4B19_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEN2078401.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="U4B19_002748"
     CDS             complement(1790..3079)
                     /locus_tag="U4B19_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078402.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
BASE COUNT          854 a          624 c          546 g         1074 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgat
//
LOCUS       DASJTC010000169         3094 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.175, whole genome shotgun sequence.
ACCESSION   DASJTC010000169 DASJTC010000000
VERSION     DASJTC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.175"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="U4B19_002749"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="U4B19_002749"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEN2078403.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="U4B19_002750"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="U4B19_002750"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEN2078404.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="U4B19_002751"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="U4B19_002751"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEN2078405.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
BASE COUNT          827 a          730 c          546 g          991 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaa
//
LOCUS       DASJTC010000170         3078 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.176, whole genome shotgun sequence.
ACCESSION   DASJTC010000170 DASJTC010000000
VERSION     DASJTC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.176"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(140..1288)
                     /locus_tag="U4B19_002752"
     CDS             complement(140..1288)
                     /locus_tag="U4B19_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2078406.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="U4B19_002753"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="U4B19_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEN2078407.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
     gene            2068..2322
                     /locus_tag="U4B19_002754"
     CDS             2068..2322
                     /locus_tag="U4B19_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEN2078408.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            2323..2640
                     /locus_tag="U4B19_002755"
     CDS             2323..2640
                     /locus_tag="U4B19_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2078409.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          981 a          513 c          541 g         1043 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg ggacaaaata aaaaggcaat acgccttagt tgtcggtctt cgagttacca
     1801 acggtaaccc tcataccgaa cggcattcat cacccaccta tagaggatgg gcgacttctg
     1861 cctaattctg ttaaatggat gttaacggaa gtgtatggtg agtagttcac tcgtattttg
     1921 atatcgtttg tatatgtttt cagcgttcta agtggcttat tttgtccaat aataatatgg
     1981 agtggaatcc cttaagaaac accgttggca attgttgtta taacatgtta taatttattc
     2041 atcaaaaact aaaaggagtg tgataacatg ggaaaagaac taaaggttag aaagatcggc
     2101 aatagcgtgg gagtgatcct tccaagctct cttggattaa aaagcggaga cattatccaa
     2161 gccaaacaag aaggcaactt attcatatta gatactactc aaatagcaaa agaacacgat
     2221 cgcaaattga ttgaagaaag tttccaagac tttgagaaag gtttaacagt ttcagaaatt
     2281 gaaatggtaa aagctttcgg aaaatacggc tggagtgaat agatgaaaga gtacacgcta
     2341 gtttattcta aaagttttca gaccagcctt gaaacgataa tagaagaatg ggaaaatgag
     2401 cttgctcttt ctccagccag cataaaaaga tttgtaggtg cgatccagaa ggctttagaa
     2461 ctaaccaaaa attttccaac tatgcatgag gaagtttccg aaatctatgg aatggatatc
     2521 ccaacatatc ggatcgtgat cggcaaaagc tatgccttat tttatcggat cgaccatgaa
     2581 aaaaaagaga tacttgtagg acgtattttc caatctaaac aaatgaaact aggcttttaa
     2641 caataattag gaaaaagtcg ctcatcctct acaggtgggt gattaatgcc gctcgatatg
     2701 gttgttaacc gtcggttact cgaagattga tatataagac ttagtacctt tttattttgc
     2761 tccatgttat ggccgaaggt ctaaagatac aatcattagt ctagagaaaa attaagaacc
     2821 gtaggaacta cggggatcgc ttggtgaata agagacactg ctattagtga aaaaataaga
     2881 tatcaagtac gctctattcc caaaaatttc gtgactttac tcatgagagg ttcaagtagt
     2941 atatttttaa tcaaaattga cgtaactttt aataaatact acctaaataa ggtagtattt
     3001 atttttgtgt tttaaaagag gaagtccaat atatctatgg gaaacttctt aaatattatg
     3061 ttggtacatg aaaatata
//
LOCUS       DASJTC010000171         2998 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.177, whole genome shotgun sequence.
ACCESSION   DASJTC010000171 DASJTC010000000
VERSION     DASJTC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.177"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            237..926
                     /locus_tag="U4B19_002756"
     CDS             237..926
                     /locus_tag="U4B19_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEN2078410.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            940..2442
                     /locus_tag="U4B19_002757"
     CDS             940..2442
                     /locus_tag="U4B19_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2078411.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2455..2931
                     /locus_tag="U4B19_002758"
     CDS             2455..2931
                     /locus_tag="U4B19_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEN2078412.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT          983 a          410 c          578 g         1027 t
ORIGIN      
        1 aaataaaatc agcgactttt ttttcagact tcgttaaaaa tggataattt tctttaatta
       61 aatagatata actcatacaa actcctctac aatttcaact tttctccatt atactccaaa
      121 ataataaaaa aaactcctaa atactccatt tttatcttta cttttatgaa agcggatgct
      181 atattgtttt tggagttgtt atccaaaaat aataaaaaat tggagtgttt aggataatgg
      241 ataaaaagag gtttttagat cttatttcaa aaggtttgat tgtttcctgt caagcattac
      301 ctggtgaacc attttacaaa gaggaagaaa gtttaatgcc ttattttgct ttggctgctc
      361 aaagggctgg agcagttggt atacgtgcaa attctgttcg agatattatt gaaattagaa
      421 agtgtgtatc attaccaatg attggaatta ttaaaagaga ttatccacca gaatctccat
      481 tcattactgc cactatgaga gaagtagatg aattagtaga aaccggtgtt gaggtaatcg
      541 ctcttgattg cactcttaga aatcgacatg atggactatc tataaattct tttattacta
      601 aaataaaaga aaaatatcct catcagttat ttatggcaga tatttctaca tttgaagaag
      661 gcaaaaatgc atataaagca ggaatagatt ttgtaggaac tactttaagt ggatatactg
      721 aggaaagtaa aaaacaagat gggccagata tagatttgat taaaaaattg gtagaagaag
      781 ggatttgtgt aattgctgag ggaaggattc atactccaga acaagcaaaa gagataaaaa
      841 aaataggagt tgcaggtatt gttgtgggtg gtgctattac tcgaccacag gaaattgcat
      901 caagatttgt taaagcatta agttaggggg agaaataaaa tgtttaaaaa attttcacaa
      961 ttaggacgcg ctttcatgtt accaattgct atattaccag tagccggatt actattggga
     1021 ctgggaggag cgttaacaaa tgagagtgct ataaatgctt atccattttt agatcaaccg
     1081 tggttgcata caattttaag tattatgagt tatgctggaa atgcagtttt tgcaaaccta
     1141 gcactaattt ttgcaattgg tattgctgta ggacttgcaa atggagataa aggaacagct
     1201 ggtttagctg gtggagtagc ttatctagta tatactgcaa ctattagtgg gtttcttgaa
     1261 cttttttcag caaaagatgc aacactagat acaggtgtag tgggatcaat tgttgttggt
     1321 ggtactgttg catttttaca caatcgttat agaaaaattg aattaccaca attcttaggt
     1381 ttttttggag gatcaagatt tattccgatt gtttctagtt tagctgcaat cattattggt
     1441 tctttctttt atttaatttg gccaccaatt caaaatggtt tagtagttgc tggtgaacaa
     1501 attgcccaaa tgggtagttt aggaactttt ttatatggtt ttttattgcg tttaacaggt
     1561 gctgttggac tacaccacac catttaccct ttgttctggt atacatcttt aggtggtacc
     1621 gaaactgtag ctggatcaac aattgctgga gcccaaaata ttttctttgc ccaactagct
     1681 gatccaaacc acactggtct gtttacttat ggtactagat tttttgcagg aagatttgca
     1741 acaatgatgt ttggattacc agcagcatgt tatgcaatgt atcgtgcaat tcctaagaaa
     1801 aatcgtaaaa agaatggtgg gctttatttt agcggggccc ttacatcatt tttaactgga
     1861 attactgaac cagttgaata tatgttttta tttgttgctc cttggcttta tgtgattcat
     1921 gcattcttag atggattatc tttttatttt gctgatatct taaatattcg tgtagggaat
     1981 tccttttcag gtggtttgat cgacttttta ttattcggtg tacttcaagg gaatgacaaa
     2041 actaattggt taaaattaat tccttttggt attgcatggg catttatata tttctttgtt
     2101 tttacattct ttatcaaaaa atttaaggta gctattcctg gaatggaaga agaatcagaa
     2161 gttcattctg aaaaagtaat aaacaaaaat acatcattac atgaagagtc tcttatcatt
     2221 attgaagctc ttggtggaaa agaaaatatt gagtctgttg aagcttgtgc aactcgtttg
     2281 cgtgttgctg ttacgaatgg ggagattatt caaaaacaaa caataaaaga cactggtgca
     2341 actgctgtct tcgaagtgaa gggtggcatc caagctgttt ttggagggaa agctgactta
     2401 ttaagtcaag aaatcaatca aatattagga aaagataatt aggaggtttt taatatgttt
     2461 gaaattttca aaaagaaaaa acaagaaact ttttatgcac cttgttccgg aaaaatcatt
     2521 tccatcacag aagtaaatga tcctgtcttt tcacaaaaat tactaggtga tggttttgcc
     2581 gtcatacctg atggttcaaa aatttatgct ccattggctg gtcgagtaac aaatatattt
     2641 cctactaaac atgctattaa tataaaaagc aactcaggca ttgattattt gattcatatt
     2701 ggaattgata cagttgaatt acaaggagag cctttttcga tttgtgttca agaaaatgat
     2761 gaggttactc ctgaaacctc gttggttgat gtggattttg acaaaataat aaaatctggt
     2821 aaagatactt ctgttatagt tgtttttccg gagaaaaatc agttcaatga tttaataata
     2881 gataatggtc gaattgctca tgggaattca tgtggaaaaa tcacttttta aaaaagtgta
     2941 tataaaaagc tataaaataa gctaaatagg atatttgtca aatgaagttg gtgaacag
//
LOCUS       DASJTC010000172         2942 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.178, whole genome shotgun sequence.
ACCESSION   DASJTC010000172 DASJTC010000000
VERSION     DASJTC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.178"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            126..2186
                     /locus_tag="U4B19_002759"
     CDS             126..2186
                     /locus_tag="U4B19_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEN2078413.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2269..2838
                     /locus_tag="U4B19_002760"
     CDS             2269..2838
                     /locus_tag="U4B19_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEN2078414.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1061 a          529 c          562 g          790 t
ORIGIN      
        1 aataaaaaat caaaaaaaat actctcctaa tagtccatct tcttttgatt ttatttatat
       61 aatgaaatca tcttatgaaa attactactt catgtttaga caagagtagg gaggactaag
      121 aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa acacgcttgt cgtttagata
      181 aggaacggtc gtgttctgaa aaacactacg ttaagtttat gatgcgcacg atgctgatca
      241 gtttatctgt aggtgtctat atgcatgtac ccgttcaggc aacgacacaa accatcaagg
      301 tcgtacgtac ctctgtttct gagaccactg attcccctat cactattact catactttca
      361 ctgatgcaaa tggaaaacag ctacttcctg cattgacttc ctataataaa gtcagttatc
      421 ctccaacgat aaaccactac tggttaattc ctaacaaaac attatggaca tcggaaaccg
      481 gaagcagtgt gccgctttct atcttttccg atatgactga tcctacaaaa ggtgatcagt
      541 taaaacagat cactgatatt ttgaatcatg ctttgacaga tataaaggac ggtggaagcg
      601 cacaagtttc ttatgtatat gaaaaagatt attcacagtt tgaaagaaag aaaatcgatg
      661 ttctggcagg gacaaaagtt tcgatatctg atctggtaga aaggctgaag aacgcagatg
      721 gaacacaagg agatatgtcc aaagtaattt caaacaatgg gaatatcgtg gatacgacta
      781 atacaggaac atctactatt tccttgtcct attttgatcc aattgcttta aaaacaatga
      841 ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc tatcaaagga aaagatcttc
      901 acttaagaac aggagagagt tacgccccta aagatcttgt tgaatatgta acaaaaagcg
      961 atggaacttc aggaaataaa gaagaaatca aaatgaacgg gacagtagat actacaactc
     1021 cgggagaata tcccgttgta ttaacttata tagactcgaa aacatcaaat gtggcagaaa
     1081 caacagctgt agtttatgta gaagcatctg ctgcttcatc gactgtcatc actcatttta
     1141 tagatagtca aacaaataaa gaacttcaag cagaaacaca tatgagtagt gtaaatcgta
     1201 ttcctgattt gcgcctacct gctgaatttt ctgatcgagt gccaaatata gatttatcga
     1261 cttatgaaca aaatgggaaa gtcacttcta tttctgattg gatgcaagca aacggattag
     1321 acagaaaaag tcactggtcg gagatccttg aagtacttca ctcagaactg atgaataata
     1381 ggaagcaatc aggaggggaa attaccttta cttatgttta tataccagac aatcgttcat
     1441 tagaaggtca tgatcttatt gttcatccaa acgaaaaaat aaacaaagaa gagttgatcc
     1501 aatcagcaac aaattcctta ggtaaacccg taccgttaaa ggatgtatcg gttacaatca
     1561 atgaagtacc attggaggat ggttatttga ccaaacaaac aggtgatttt acgattatgt
     1621 atagttatca tgacccgtat tcactccaag aactccatgc tcagtcacta ttacatgttt
     1681 taccattaaa caaacagata gatcaaaaag aacaaaaaag acaagaagaa agcttgtcag
     1741 aaaaaaaaca acaaatggct catgcagttt ctccaacagg gaaagtaaaa caaacaagaa
     1801 gcctagagtt agcaagaata tctagaaaaa tctcagaatc taataaaacg ataaggcaag
     1861 aagctcattt ggccccacaa gtttctggat tggaaaatga aattttagag cctaaaataa
     1921 aacaacaatc agaaaaagaa tttgttgcta aacagtcgga agatcagaag gaatcagatg
     1981 accagaagga aacgaagaaa aaacgcttgt caactattga ttccaccgat gagcatctgc
     2041 agccgcaagt accacttcct agtccgggaa cacccgcacc tggtggaggt attccttctt
     2101 ctgctttagg agatttcatg cgaggtatgg caggaacatg ggtatacgct aatcgaaatg
     2161 aatttgacgc agatgactgg ttctgattta tatctctatc tgttatagtc aaataagtag
     2221 ctgagtcata tgttatttgg cataatggag acgaggaggg atagaaaaat gttagataaa
     2281 acgttacctt acgcagaaat ctggatgaca agaccacttc aacaaaatgt tccaatcacg
     2341 cctttagcgg aaggttttga atttcgattt tatcaaaaag aagatgaatc tgcttgggcg
     2401 gagattgaga cttccgttgg ggaatttcaa acgacagatg aagcacttga gtattttcaa
     2461 caaaactttg ctccttatcc tgaagaactt gaaagaagga tgttttttgt agagacaaaa
     2521 aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa aatcaagaga gcaagtgtat
     2581 ccggtttttc attggttagc agtgaaatca gattaccaag gaaaagggct cgcaaaagca
     2641 ttgactgcca gagtcttgca agaatttcct accctccata cggacggacc aatctatctt
     2701 cacacacaga catggagcca tcaggcgatt gctctttata aaaaaatggg ttttactttt
     2761 attgctgaaa atctagacgg cacacctaat ccagattatg aaaaagttat gcaggtttta
     2821 agtaaattag ataaataacg aaatggtgaa ataagtcctc agtattggga taaaaaacga
     2881 aatagtcatt cctcatatct tacttttttg agttaatact atcggataac taatggcatg
     2941 cc
//
LOCUS       DASJTC010000173         2677 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.179, whole genome shotgun sequence.
ACCESSION   DASJTC010000173 DASJTC010000000
VERSION     DASJTC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.179"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            692..787
                     /locus_tag="U4B19_002761"
     CDS             692..787
                     /locus_tag="U4B19_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEN2078415.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1089..1334)
                     /locus_tag="U4B19_002762"
     CDS             complement(1089..1334)
                     /locus_tag="U4B19_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078416.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1679..1963
                     /locus_tag="U4B19_002763"
     CDS             1679..1963
                     /locus_tag="U4B19_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078417.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1966..2382
                     /locus_tag="U4B19_002764"
     CDS             1966..2382
                     /locus_tag="U4B19_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078418.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          882 a          498 c          430 g          867 t
ORIGIN      
        1 tccgtttttt catccattcc tttcttcccc ccttacaaat aaataataac ctttaacaac
       61 caactcgtaa ataggttggt tgttattata caaaaggata ttagtactca gataaaaagt
      121 tgttagtact cagataaaaa gttgttagta ctcagataaa aagttgttag tactcagata
      181 aaaagttgtt agtactcaga taaaaagttg ttagtaccct tctgtatccg ttgggagagt
      241 aaggctcaac tgccccctaa aaagggttct aaaaaatctt taaaaaataa ataaaataaa
      301 cgcgcgcgcg cgagcaaaat ttcccgccaa aaaatcaccc actttttaaa ataaacaggt
      361 tcgcgcatga tattaaactg ccaatctagc tccctatggt cgctgtaaga aggagtgatc
      421 gctacgctct cacattttac ttgcagcagt cgcctttagg ctccttcgcc aaaaataatt
      481 cattagggac tcctttgtgg cgtcctgaga gctcgtctaa gccgttctaa gacaagattc
      541 gataaaaact atgtccttcg ttctaaagcg ccttacaggc ggtcagggca agccctaaca
      601 accccaaggc aataaaaaac ttaggcttat ttttgctttt ttattgtata attgattgtc
      661 gcctatccga cagtcttgcg actggaagga ggtgtgtttc gtgttcacta actttatttg
      721 ctcccttctt gttgggcttt taagcgcttt gttcagttac tggttgaata accgaaacaa
      781 gaagtaaaac ttaattaagg cgactcagcc cacaaaaaaa gccccagaga atccgacctc
      841 tctggggctt tcgtgtgttt cgtgttcact tatttgcaat caaattataa cagaaataaa
      901 tatttatttc aaaaatatcc tacaaaagtc taaaatccgt aattatttct tatatgcaac
      961 atttacaaat aaagtcgctc tgagagctcg tatttttcga tttaagacgt tttatttagt
     1021 ttttggtgta tttcattggt ccaagctaaa aaactcttag aagcgatttt agagccattt
     1081 aaacccactt aattatattc tccatgaaat tcgctccatg cgtcaaaagc tgcgtctgaa
     1141 attccgccgt cttcgttcac tagttgcatt aatttttcca acgatcgttt tctttcgggt
     1201 gtcatatttt tatattccaa caatttttcg tctgttaaat cgaacttgat tgcttgaatt
     1261 gcttgctcta atttttctaa gttttgaaaa tattcgtaag gaaatttttc cattttttct
     1321 ttctccagtc tcacaatttt tctagaaata aaatcacttc tttgccgtct atactctcaa
     1381 aaaaaatgtc gtttgaaaaa ctaaataaat ttttgatcgt gcatttttaa atttggttat
     1441 cgattacacg tgcagcactc ccccccgaat tttaaagagc ataaggaaac gtgtacccac
     1501 tttctaaaaa aagtgggtag ataggctgtg cctatcacgt ttctcagaaa aaaatttgat
     1561 atcacttttt cgccatcgtg ggtagcttat gtatagcatt ttggcaaaaa acagctatac
     1621 aaatatagta tttgtatggt atacataaat tatacataat tgcaaaagga gattttttat
     1681 ggacatttta gttcggaata ttgatccaaa gtttgttgca acaatagaca aacgttgcca
     1741 agaactcacg aataaaacag gagaaaaatg gagtcgaaat aaatatctaa aagttttaat
     1801 cgaaagtgat tttgaccgtc aacttacgac ttacaaaaaa gaccaattcg atttattgct
     1861 cgataaattt attgctatcc aaacagaaaa tactcaaata ttaagcgagt atgtctacac
     1921 aaataatcaa atcattgaaa tgcttatcgg aggaatgata taaccatgag aaaaacttct
     1981 gtccaaaaac atgttacttt gtaccctagt catgttgacg ttttagattc catcatcgaa
     2041 aaaaatccag atatcaataa ctactcggaa gccattcgct ttttatgtat gagttatgaa
     2101 cccgatacca acacgaccac taccagcaat caggcaaaac taaatgctat gggaaaagaa
     2161 atttccatca tgactgaaat catcgcagaa tttgctgatt tatatctaaa agatacaggc
     2221 gttcttattg gcacaaattc taaagtatat caagaagtga aagaaaaaat cgaagctaaa
     2281 ctaaatccat caaatcgaaa tcgaccaaag aatcatcaaa aagaaaccag tcaaacagtc
     2341 acttctagaa atttcgaccc aacaaatttt ctaaaacgat aaaaaacaga taggagttat
     2401 tcctatctgt tttttattta gaagccccat acaatatgct agaatgtact agcaatctta
     2461 gtcggttgta agggacttgt ctacttgctt aatgtaaggg gggcgagttc ttttttattt
     2521 attcttctaa ccagttacgc agagaaatct ttggcaaatc tgacgttagg cttgaaaaag
     2581 taaattctaa tttggcgatt ttattgccct tttttgcttt tatttttgtg actttaagat
     2641 cattgaagta tttttttagt tctcgtaatg caggatt
//
LOCUS       DASJTC010000174         2667 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.180, whole genome shotgun sequence.
ACCESSION   DASJTC010000174 DASJTC010000000
VERSION     DASJTC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.180"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..623
                     /locus_tag="U4B19_002765"
     CDS             <1..623
                     /locus_tag="U4B19_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEN2078419.1"
                     /translation="GAGGHAKVIIETLRLENKLIYGFFDDKQNKMQDLKYLGKINELN
                     SWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIAVHPSAIVAKEISIGYGTVI
                     FANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPGVCLAGGVHVGVGTQIGIGS
                     QCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKKEGINFEF"
     gene            613..1755
                     /locus_tag="U4B19_002766"
     CDS             613..1755
                     /locus_tag="U4B19_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEN2078420.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            1768..>2667
                     /locus_tag="U4B19_002767"
     CDS             1768..>2667
                     /locus_tag="U4B19_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEN2078421.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLE"
BASE COUNT          958 a          339 c          531 g          839 t
ORIGIN      
        1 ttggggctgg cggacacgca aaagtcatta ttgaaacctt aagattagaa aataagttaa
       61 tatatggatt ttttgatgat aagcaaaata aaatgcaaga tctgaaatat cttgggaaaa
      121 taaacgaatt gaatagctgg gcggatagca aaaaaaaaat ctataaattt attgtagcta
      181 ttggtgactg tgtagtaaga aaaaagatta taaaaaaatt gaatatgcca agtgagcaat
      241 atggaatagc tgttcatcca agtgctatag tggcaaaaga aatttcaatt ggttatggaa
      301 ctgttatttt tgcgaatgct gtaattaacc cagatgctgt aattggagag catgctataa
      361 taaacacagg tagcattgtt gagcatgatt gtagaattaa taactatgtt catttatctc
      421 caggtgtatg cttagcgggt ggagtacatg taggtgtagg aactcaaata ggaattggtt
      481 ctcaatgtat ccagatgaag aaaattggat cttggtgtat gataggcgca gggagcacta
      541 ttgttaagga tattccctca cattccttag cttatggcaa tccagctaaa attaagaaag
      601 aaggaattaa ttttgaattc tagaatttta ttatcaagtc ctcatatggg aggaacagaa
      661 gaaaaatatg ttgctaaggc ttttaaagat aattggatag cgccattagg tgaaaatgtg
      721 gataaatttg aagaaagtat tacaaaatat actggttcta agggcggagc ttctgcgtta
      781 agttcaggta cagctgccat acatcttgcc ttaagactgc ttaatgttgg aaaaaacgat
      841 attgtctttg ctccttcatt tacttttata gctacagtaa atcctatttt atatcaaggg
      901 gctgtaccag tgttcattga ctcagaacct aatacatgga atatgagccc tgttgcacta
      961 gagagcgcat tagaagaagc tgaaagaaac aaccaattac ctaaggcaat catcgtagtg
     1021 aatttatatg gtcaaatggc tgattatgaa cgaattttag ctattgcaaa ccaatttaat
     1081 gttccagtga tagaagatgc agcggaatcg ttaggttcta cttataaggg aatttcgtca
     1141 ggcttatttg ggacaattgg gatctattca tttaacggta ataagatcat cacaacctca
     1201 ggtggaggag ctctagtgtc tagtgataaa aaatatgttg agcattcaag atttttagca
     1261 acacaagcaa gagatcaagc accgtattat caacatagcg aaataggtta taattaccgt
     1321 ctaagcaata tttctgccgg tatcggacgt ggacaaatgg aagtattgaa tgatagagta
     1381 gctaaacgaa gagaaatttt caatcgatat aaggaagctt tttctggaat tgatagaatt
     1441 gaaatgatgc cagaagtgga tggcatggtg ccaaatcgtt ggttgtctac tttaacgatc
     1501 aatgattata attctattga tcaagtagtt aacgtaataa ataaactaaa tgaaaaaaac
     1561 attgaaacaa gagctttgtg gaaaccacta catttgcaac cagtatttca aggggtaaaa
     1621 atgtattacc atgataataa tcttagtgtg tcagaaaagt tatttaatag cggcttatgc
     1681 ttaccttctg gaagtaatat gacagatagt gaacaagaat ttgttattga tgagtttaaa
     1741 aaaatattca agtaggtgtg atattttatg aacataggaa ttattggttt aggttccatg
     1801 gggaaaagaa gacttagact cattcaagaa cactttaatg atattttact atgtggtgtt
     1861 gatactaatg aggcgagacg aaaggaagtt gaaaaggaat ttggaatacc aacttataat
     1921 agtctaacag aggcaaaaaa tttgtttgaa ataaattcag tttttgtttg tacatcccct
     1981 ataacccatg aatttattat aaaagaggct ttaaatttag attgtaatat ctttacggaa
     2041 atcaatttat taaatgaata ttatgatgaa ataatcgaag tagctaaaaa gaaagataga
     2101 ttactttatc tatcttctac atttttgaag agaaaagaaa ttcaatatat taagtctaaa
     2161 gtgacgcata ataaaaaaat ttcttatcaa taccatgtag gacagtattt acccgactgg
     2221 catccttggg aagattatcg caattttttt gtttcaaaca aaaaaactaa tgggtgtaga
     2281 gaaatattcg ctattgaatt accttggatt atagatacat ttgggaaagt taaatcgttt
     2341 actgtaatga cccagaaatt atccaatctt gatattgatt attcggacag tttcactctt
     2401 attattgagc atgaaactgg tatagttgga actttaagtg tagatgttgt ttcaagagta
     2461 gctaaaagaa atttaactat aattggtgaa aagattttaa tagaatggga aggtaacccg
     2521 gattctctat tttcatggaa tgaagaaact tctagtatga cagatattca attgtacgat
     2581 gaggttatgc atgataataa ttattcacat actattattg aagatgcata tttagaggag
     2641 attaaagaat ttttcacgct tttggaa
//
LOCUS       DASJTC010000175         2586 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.181, whole genome shotgun sequence.
ACCESSION   DASJTC010000175 DASJTC010000000
VERSION     DASJTC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.181"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1416
                     /locus_tag="U4B19_002768"
     CDS             <1..1416
                     /locus_tag="U4B19_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEN2078422.1"
                     /translation="YEPTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTID
                     TNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTD
                     NVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRS
                     NADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVV
                     TYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTT
                     YTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIV
                     TGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPT
                     NVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISG
                     GLILLGTLGLLGYKNRKKENE"
     gene            1550..>2586
                     /locus_tag="U4B19_002769"
     CDS             1550..>2586
                     /locus_tag="U4B19_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEN2078423.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKT"
BASE COUNT         1015 a          361 c          519 g          691 t
ORIGIN      
        1 tatgaaccaa cagtagaagg cgaaaaagta gaagttgggg gcacagtaga tctaacagat
       61 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      121 ggtacaattg ataccaacac accaggtaat tacgaaggtg tcattgaagt aacgtatcca
      181 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctgacgcc
      241 gataaatatg aaccaacagt agaaggcgaa aaagtagaaa ttggaggcac agtagattta
      301 acagataatg ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact
      361 cccggcggta caattgatac caacacacca ggcaattatg aaggtgtcat tgaagtaacg
      421 tatccagacg gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct
      481 aatgcagata aatacaatcc tgaaggacaa aaagtgacta cagacttaaa taaagagcca
      541 gatgcatctg agggaattaa gaacaaagaa gatttaccaa aaggaactac ttatgcttgg
      601 aaagagaaag tagatgttag cacagctgga aataaaaaag gtactattgt agtgacatat
      661 ccagatggat catctgatga agttgaagta gacgttacag tgacagatac tcgttccgat
      721 gcagataaat ataatcctga aggacaaaaa gtgactacag acttaaataa agagccagat
      781 gcatctgagg gaattaagaa caaagaagat ctaccaaaag gaactactta tacttggaaa
      841 gaaaaagtag atgttagtac agctggaaac aaaaaaggta ctgtagtagt aacatatcca
      901 gatggatcta aagaagaagt agaagttact atttctgtag tagataaaaa agcaccgaat
      961 aaacctcaag ttgatcctat tacagagggt gaccaaattg ttactggtaa aactgaacca
     1021 aatgcagagg tgacagttac attaccagac ggaagtcaac accatgggac agctgataaa
     1081 aatggtaatt ttacagttaa agttcctaaa ttagaggcag ggacaaaagt gatagtaact
     1141 gcaactgatg aatctggtaa tactagtgaa cctactaatg tagttgtttc ttccaacaaa
     1201 aaagacagtg gaaaaaacgg tagtaaaggt agtaagactg acaatcaagg tagcaacagc
     1261 aatcaagaca aaaatagagg caaaagccag tcctcaaaag tttttcctaa gacgggagag
     1321 agtgacagta atatctttac tattagcggt ggtttaatac tgttaggaac gttaggatta
     1381 ttagggtata aaaatcgaaa aaaagagaat gaatagaaaa tcaatttctt aagtcttctc
     1441 tgagaagagg aagaatcttt ttcttcctct ttttgctttt ttatgatggg aaataagtgt
     1501 tagttaaaga tgaatatact ttttatagaa aggaaaagaa tgtaattaag tggaaaaaat
     1561 agtaattatt ggttcaaacc attcagggct agccgcagct cgatttctaa acttaagcga
     1621 ttcaaattat gaagttattc taatcgataa aaaatcagaa attagttact tggaatgtgg
     1681 gacatcgtta tttataaaag gagaagtaag taatttagaa gatctttttt atcaaaatag
     1741 aatgacacta aattctgaga taaaaaagtt atttctagag acagaagttg acaaaattga
     1801 tattcaaaat aaaaagatta gttgtgtaaa aaaagatggt acaagattct ctcaagaata
     1861 tgacaaagtt atattagcta caggttcaaa gcaaattatg ctagatatac cggggaataa
     1921 tttagctaat attttttcga taaagaattt tccaaatgca aaagagattt ttaaagcttt
     1981 ggatgatagt actattaaaa aagtaggaat tattggagca ggatatattg gagttgaaat
     2041 tgctgaagcc ataagaaaaa gagggaaaga agtatatctt ttcgatgttg cagatagagt
     2101 gttgtcgact tattacgacc gtagtttttc agataaagtt gaagaaatac tatcaaaaaa
     2161 tggaatacat ctttgtctat cagaaagtcc taaaaaatat ttaggaagaa aaaagattga
     2221 aaaaatagtc actacaagtg gtacccaata ctctatggat atggtcgtac aagctatagg
     2281 atttctatct aatagtccaa ttggggaaaa ggaactcttg agagatgtta gtggagcata
     2341 tctaactaat gaataccagc aaacaaatat aaaagatata tatgctattg gggattgtgc
     2401 tacaactttt tttacgtcca ttaatagaag tagtgttgat ttttctattt ctaatgctct
     2461 aagaacagct tatattgcaa ctcaacatat taacaatcaa gattttacac cttctggttc
     2521 ccaatttaca aatggctttt ctatatttaa ttacaatttt tacgcagcag gtttgaattt
     2581 aaaaac
//
LOCUS       DASJTC010000176         2579 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.182, whole genome shotgun sequence.
ACCESSION   DASJTC010000176 DASJTC010000000
VERSION     DASJTC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.182"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..2258
                     /locus_tag="U4B19_002770"
     CDS             <1..2258
                     /locus_tag="U4B19_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEN2078424.1"
                     /translation="DKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSGLYFNS
                     NEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQRIVEQ
                     LDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEGLSVLA
                     KDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNLKLETF
                     ELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQDGQSI
                     VLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLNLIELL
                     ISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEEEYPTIFEI
                     DYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDKKAEHY
                     RQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRFPELSV
                     SITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPSRLREDILI
                     FVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKRDYDNRNSA
                     NDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHDPENGN
                     MYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELDGLKQASSY
                     LFKFKQKRSDG"
     gene            2393..2521
                     /locus_tag="U4B19_002771"
     CDS             2393..2521
                     /locus_tag="U4B19_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEN2078425.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT          985 a          339 c          495 g          760 t
ORIGIN      
        1 tagacaaatt agatacgaaa gacattgtca attggctcaa tcaagacgaa gaattgacag
       61 atttattatc ttcggtaaaa aaagaatcaa gtgcaagtca gcaatcgaac tggagtggat
      121 tgtactttaa ttcaaatgag tatgtgaaat acattttgaa taaaactgca tggattaaga
      181 ttgatggaga aaaatatagc ccgagacaaa ttgtgctata tgaaaagctc caggcacacg
      241 ttccgggatt gtatggagtt tctgaacaaa gcttaatgga actcttgggg caacgcattg
      301 ttgaacagtt ggatttcaga aaaaatatgg cgatgttttc ggatgatgaa ataagagcta
      361 tattagttga gttaccaaaa ttcgataaag gcgaaataag tcgcagatta tataatgaac
      421 ttataagaaa taaaaaagga atgaatccta cttattctac cgaaggatta tcagtcttag
      481 ccaaagatgg cttcttttat ccaaacaaag aattaaggta tgctgataag cgactgccta
      541 aatcaataga aaaacaaatg cgttttattt acgtgcctga aaaagctagt acaactacga
      601 tatatgaatg gcttggggtt gaacgcttca aaacaaattt aaaattggaa acatttgagt
      661 tattggaaga ttattctgag gatttcaaaa aagagattga tgatatcaaa actgcggtat
      721 tatctacgat agatgacaat agtaagaatg ttagttcatt aaaacgtatt cagattattc
      781 catgcagcaa aatatctgct aaagatgttg agcaagatgg tcagtcaatc gttttggaag
      841 attattattt tgtggaaagt aaaggaaaat tctacttaaa attacctgct aattctattg
      901 aaattaatca attacgtttg attgatacgt tttcttcaac aatcgtagac atattcaagc
      961 aggtgttaaa tctaagctta gatttgaatt tgattgaatt gttaatttcc cgagatacgg
     1021 aacataaaca aagaaaaata actgaagaat tcggtgtaga taagtggaat gaatcgtttg
     1081 agctattgtt tagcgaaaat gcgttaaata aaaaagttaa tgactttttg ttatcaaatg
     1141 gactaaatga agaggaatat cctaccattt ttgaaattga ctattcatat gcattaaaag
     1201 aagaagagta tcaattgttg gcgatagctc tagaaagcat ttcaaaagat attgaggatt
     1261 tgaatggatt gtcagaattg attcatttgg atattcgtga attttggaaa ctgaaattaa
     1321 gatcttatct agataagaaa gcggaacatt accgccagat tttatatagc aatgcaaata
     1381 aaagcggtga tgaaatactc aaagaaacat tcctagaaaa aattgataga tacaaagatt
     1441 atcaatttga gatttctgaa attccaaatt cagttcaagt cgacattgag aaagaaagta
     1501 agaagagatt tccagagcta agtgtgtcaa ttactgatga aattgacatt gatggcctgt
     1561 atgataataa tgtcgaagag ttagtcacgg ttgcagctat tacaatagat gattttgatt
     1621 attatattag aggtcatcaa aaagtttctt ccttactgta ttttggtatt ccgtcccgtt
     1681 taagagaaga tatactgata ttcgttacta aagaaaatga tgttgatttg aacggagaaa
     1741 aatctggtat ctctattgaa ggaacatcaa ctgttcaaac cattcttgaa ccatcgaata
     1801 gtgttagtag gaaaggtaag aacacacgaa atggagaaag aagtaaacga gattatgata
     1861 atagaaactc cgcaaatgat gatgctggtg aaacagcaga acaaattgct tacgctgaat
     1921 tgactcagaa tcaaaaactt aatgtaattt ggaatacgaa atattctagt aaatcttcag
     1981 atcgtaataa gcagccacca aacggaattg tttgtgatat gtgggttcat gacccagaga
     2041 atgggaatat gtactttgag gtaaaatcat caattacaga atttgaaatg tcgattaatg
     2101 aatataattc tatgttagat aaccctgact cttacgaggt cgttttagtc aatcgtgata
     2161 ccaaagagat aagtcgtcat aaatttgatg aacttgatgg attgaaacag gcaagtagct
     2221 atcttttcaa atttaagcaa aagagaagtg acggttgagt gatttgtttc cacaaaccta
     2281 ttcaatctat caactcaaaa atcataaaca accattatta tagtaggaag atacagagtg
     2341 tactctcaac gcatatcgaa ttagtcgctt cattcgttaa aggagaataa aaatggacca
     2401 aaaagaaaaa atgaaagaat tgatcaaaaa gaaacaaggt ggaggccgat ccaaacaaat
     2461 ggaaacacct aagaatgatc gtaaaaacat gagaaaaggc cctaaaatct ttaataaata
     2521 gacgtgctaa agaaagacta agaagtgtta acttttgtta ttgtttctta gtcttttta
//
LOCUS       DASJTC010000177         2550 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.183, whole genome shotgun sequence.
ACCESSION   DASJTC010000177 DASJTC010000000
VERSION     DASJTC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.183"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            44..2485
                     /locus_tag="U4B19_002772"
     CDS             44..2485
                     /locus_tag="U4B19_002772"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEN2078426.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT          847 a          457 c          605 g          641 t
ORIGIN      
        1 ttttacagca aagaatggta tatttttgta ggaggtggct tacttgaaag aagtcgttat
       61 gatagatgct gcaagaacgc cgataggaaa gtatcgcgga agtttaagtc catttactgc
      121 agttgaatta ggcacacttg tgactaaagg attattagat aaaacaaaac tgaaaaaaga
      181 taaaatcgat caagtaatat ttggaaatgt cctgcaagct ggtaatggtc aaaatgttgc
      241 cagacagatt gctttgaaca gcggtctccc tgtagatgtc ccagcaatga cgatcaatga
      301 agtttgcgga tctggtatga aagcagtgat cttagcacgc cagctgatcc aattaggtga
      361 agcagaactt gtgatagccg gcggaacaga gagtatgtct caagcaccta tgctgaaaca
      421 gtatcagtca gaaacaaatg aatatggtga accaatttcc agtatggtca acgacggatt
      481 gactgacgca ttttcaaatg cacatatggg attaaccgca gagaatgttg caacacaatt
      541 ttctgtgagc agagaagagc aggatcgcta tgccttgtcg tcccagttga aagcagcaca
      601 tgctgtcgaa gccggtgtat tttctgagga gatcatccca gtcaagattt ctgatgaaga
      661 cgtgttatct gaggatgaag cagttcgtgg aaatagtaca ttggaaaaac tgggcacgtt
      721 acgtacagta ttctcagaag aaggaactgt aacagcagga aatgcttccc cgttgaatga
      781 cggtgcctct gtggtgatcc ttgcatccaa agaatacgca gaaaataata atctgcctta
      841 tttagcaacc atcaaagaag tagcggaggt cggtattgac ccaagtatca tgggaatcgc
      901 tccaatcaaa gccattcaaa aattgacaga tagatcagga atgaatttat caacaatcga
      961 tctttttgag atcaatgaag catttgcagc ttcttctatc gtagttagcc aagaactgca
     1021 gttagatgaa gaaaaggtca atatatatgg aggagcaatc gcattaggcc atccaattgg
     1081 tgcaagcggt gcacgtatct tgactacatt agcttacgga ttgcttcgtg aacagaagag
     1141 atacggtatt gcttcattat gtattggtgg aggtttaggc ttggcagtct tgttagaagc
     1201 gaatatggag caaacacata aggatgtgca aaaaaaaaag ttttatcagc ttacgccatc
     1261 tgaacggcgc agtcaattaa tagaaaaaaa tgtattgaca caagaaactg cgttgatttt
     1321 tcaggaacaa acactttctg aagagctctc cgatcacatg atcgaaaacc aagtgagtga
     1381 agtggaaatc ccaatgggga tcgcgcagaa ttttcaaatc aatggcaaga aaaaatggat
     1441 tccgatggca acagaggaac catctgtcat tgcagctgca agtaatggtg caaaaatctg
     1501 tggaaatatc tgcgcagaaa cacctcagcg gctgatgcga gggcaaatcg ttctatcagg
     1561 aaaatcggag tatcaagcag tgatcaatgc agttaatcat cgaaaagaag agctgattct
     1621 ttgtgcaaat gaaagttatc cttcaatcgt gaaacgcggg ggcggtgttc aagatatctc
     1681 tactcgggaa ttcatgggga gtttccatgc atacttatct attgattttt tagtggatgt
     1741 aaaagatgca atgggcgcta acatgatcaa ctcgattctt gagagtgtgg caaacaagtt
     1801 acgggagtgg ttccctgaag aagagattct attcagtatt ttaagtaatt ttgctacaga
     1861 atcattggct tctgcctgtt gtgaaatccc attcgaacgc ttagggagaa acaaagaaat
     1921 cggtgaacaa attgccaaaa aaatccaaca ggcaggggaa tatgcgaagc ttgaccctta
     1981 ccgtgcagca acgcacaaca aagggatcat gaatggtata gaagctgtag tggcagctac
     2041 aggaaatgat acacgtgcgg ttagcgcttc gatccacgcc tacgcggcaa gaaatggatt
     2101 ataccaagga ttaacagact ggcagatcaa aggggataaa ctagttggaa aactaacggt
     2161 gcctttggct gttgccacag taggcggagc ttccaatatc ctgccaaaag caaaagcatc
     2221 tttagctatg ttagatattg attcagccaa ggaactagca caagtaattg ctgcagtagg
     2281 gttagctcaa aacctggctg ctttgcgtgc attagttaca gaaggaatcc aaaaaggcca
     2341 catgggactt caagctcgtt cattagcgat ctcaattggg gctattggtg aggaaatcga
     2401 gcaagttgcg aaaaaattac gtgaagcaga aaagatgaac cagcagacag ctatacaaat
     2461 attggaaaaa atccgggaaa aataaaaaag caatatcttt agaagaataa agcaagattt
     2521 acagttattt tatttactgc agatcttgct
//
LOCUS       DASJTC010000178         2520 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.184, whole genome shotgun sequence.
ACCESSION   DASJTC010000178 DASJTC010000000
VERSION     DASJTC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.184"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            194..>2520
                     /gene="esp"
                     /locus_tag="U4B19_002773"
     CDS             194..>2520
                     /gene="esp"
                     /locus_tag="U4B19_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEN2078427.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT          918 a          388 c          506 g          708 t
ORIGIN      
        1 tgaatggtta aaaagcacaa atatccgaaa aaaacatgaa gaaatgtact ttttttgttg
       61 taatgttcat gaaattctct aagtaaatta gttaacataa ggttagtaag attcctattc
      121 tgtgaataat acgtatcaga tttatctttt attcttacta ggaaagtaat tattaaggga
      181 gttgatttga ataatggtta gcaagaataa taagagagta tttctagaaa aaacaaagaa
      241 acgagtattg aagtacagta tcaaaaaatt aagtgttggg gttgcatctg ttttgattgg
      301 tgttggtctt gtcttgggaa caactgaact agttaaagca caagatgaaa tttctccaag
      361 cagcccactt gaaacagcga taagctccgt acaagtaggt gacaaagtag cttcggggaa
      421 tacttttcag gagaatcctg gttatacgaa aaattataac ttttcggatt tacaatttag
      481 tcctcaagaa ctaactggag acacactaaa aggcaataca attggatttg aagtttacgg
      541 aaaacataat attgcagcat caactaaaaa ctgggaaatc cgtcttcaat tagatgaacg
      601 attagctaaa tatgtagaaa aaattcaggt cgatccaaag aaaggaattg gatcttctag
      661 acggaccttt gtaagaatta atgattcgct gggtagacct acaaatattt ggaaggtcaa
      721 ttatattcgt gcaagtgatg gattatttgc tggggcagaa acaaccgata cgcaaaccgc
      781 tcctaatggg gtaattacgt ttgaaaagag cctagatgaa atttttaaag agataggcat
      841 agacaatctc aagaccgatc gtttaatgta tcgaatctac ttggtaagtc atcaagacga
      901 tgataaaatt gtaccgggta ttgatagtac gggctacttt ttaacagatt cagatgattt
      961 ctataatagc ttagatgtat ctgaaaataa tcctgaccag tttaaacatg gatcagtaag
     1021 tgcaaaatat gagaaaccta atactcaaac aaaagatggt tcaggatcca caggtgctaa
     1081 tggagctata atcttagatc ataagttaac gaaaaattat aatttctcat attcatcttc
     1141 cgcaaagggc acgccttggt atgctaatta caaaattgat gaacgattag ttccatatgt
     1201 agcgggtata cagatgcaca tggttcaggc tgacaaagta acatatgatg tttcgtttga
     1261 atccggaaca aaagtagctg atttagcaat cgagcgacgg gaaggtcatg aaaattatgg
     1321 cataggttca ataactaata atgatttaac taaacttatc gattttgcta atgcaagtcc
     1381 acgtccagtc gttattcgat atgttttaca attgactaag ccattagatg aaatcttaga
     1441 agatatgaaa gcaacagcac aagttgaaga aaataaacca tttggtgaag atttcatctt
     1501 tgattcttgg ttgtcggata cgaataaaaa attaatccag aacacttatg gaacaggtta
     1561 ttattatttg caagatattg atggtgatgg aaaccctgac gataaagaag agagcggaga
     1621 cacgaatcca tatatcggga aacctgaatt agaagaagta tatgatgttg acacaacagt
     1681 taaggggaaa gtattcatcc acgagttagc gggaacaggt cacaaagccc aacttgttga
     1741 taaagaaggt actgtattag cagaaaaaac tatcgctcca aatgaaaaag atggggctcc
     1801 aatttcagat actgtagaat ttgaatttac gggtgtagat tcaagtaaat taatcgcgaa
     1861 agatgaatta aaaatccaaa tcgtttctcc aggttttgat aaaccagaag aaggttcaac
     1921 cgttattaag gaatcaccaa aagcggttga taaacaaacc gtggtagttg gatttaaacc
     1981 agatgctaaa gaatcaattc ggaataataa aaacttacct gaagatgcag agtattcatg
     2041 gaaaacagag cctgatactt ctaacgttac tgatagtacg aaaggtattg taactgttaa
     2101 gatcggaaat cgaactttcg acgtggatgt agagtttgct gtaaaagctt ctcaagctat
     2161 ggaaaatgat gcaacatacg tacctataac aacaactcca gaaacgacag ttcaaagtgg
     2221 aaaacctaca tttgataaac cagatgttcc tttagctaaa gatgcctttt caatttcaga
     2281 tgtctataat aagtacttcg gtaatgcaag tgttgatgca aacactggta ttgttacatt
     2341 cactccagct aaaggtgtag gagaatcgga gccgattact ggaacaattc ctattaaaat
     2401 tgtttaccaa gatggttctg taggcacgac cgatttagca gtaactgtaa gtaaagatat
     2461 ttatgaaaat ccaggagaaa acattcctgc aggctaccac aaagtaacct tcaccgcagg
//
LOCUS       DASJTC010000179         2470 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.185, whole genome shotgun sequence.
ACCESSION   DASJTC010000179 DASJTC010000000
VERSION     DASJTC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.185"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..385
                     /locus_tag="U4B19_002774"
     CDS             <1..385
                     /locus_tag="U4B19_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078428.1"
                     /translation="ISEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSE
                     IEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYA
                     VVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            399..800
                     /locus_tag="U4B19_002775"
     CDS             399..800
                     /locus_tag="U4B19_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078429.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            802..1173
                     /locus_tag="U4B19_002776"
     CDS             802..1173
                     /locus_tag="U4B19_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078430.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1209..1508)
                     /locus_tag="U4B19_002777"
     CDS             complement(1209..1508)
                     /locus_tag="U4B19_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078431.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1514..1744)
                     /locus_tag="U4B19_002778"
     CDS             complement(1514..1744)
                     /locus_tag="U4B19_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078432.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(1994..2194)
                     /locus_tag="U4B19_002779"
     CDS             complement(1994..2194)
                     /locus_tag="U4B19_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2078433.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          891 a          356 c          411 g          812 t
ORIGIN      
        1 aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata aagtaattaa
       61 tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt ctgtaacagc
      121 tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg aagttttaaa
      181 caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc ctcctcaagt
      241 taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag caggagagca
      301 ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg atatcaaggt
      361 caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata gtaccatcaa
      421 atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc gcatcggtaa
      481 ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa aatgataatc
      541 aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat ggcaaaattt
      601 ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa tatgatgaag
      661 atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc tcagattacg
      721 atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg aaatctgtag
      781 tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat ttccaaacaa
      841 attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc tgtttcaaga
      901 gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta tgatattatt
      961 tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt ttcaacagtt
     1021 aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga gaaaagaaaa
     1081 tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct gatgaatcga
     1141 gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt tgtttgttat
     1201 ttttttattt aatctttgta tctttctgga tcaacgaaag tatactttat atagtcataa
     1261 cgccgatgat cgctacgtgc gtccggcacg tcagtcacga tatcaaacaa aaagtataca
     1321 cccttcttca ttctagtttt cgcagcagga attttgaaat agttcttatt agaatagtag
     1381 agattgatta ataagctatc ttcgattgct aaaaagaaaa cttctgaatt ccatacttta
     1441 taaaaatctt tgataaatct attcgaaggg tcaaatttaa accataattg tgtctcatta
     1501 aaaagcataa ccattactcc gatctgtttt taaaattagt ttctacctct aatatatatc
     1561 gagtttttat tttgccttca gagaatactg tttctttttt tgcagttaca ggttgtttat
     1621 tttcggaaaa agctaatata gctaaaattg aaacatccat ctggaatttg tctttttcgt
     1681 tgctttgctc ataaaaatct gcatattcat cactaatatt ttttctaata aattcttcca
     1741 tcataaagat cacctcgaaa agagtatacg aacaaacgtt ctttttgtaa aggtggaatt
     1801 tattatacta aataaatagg tgaacaagta cttgtgccaa attatgtgcc aaaaaaaatc
     1861 gaatttaata aaacacaaac aaaaagaaat cctattatga taacatttct tataataaca
     1921 aacacaacag aagacgtgta atagttagtc aggaacgtac aaataacccc tgtatccttt
     1981 gcggtacagg gggttatttt gtataagtag acattgtagt ttctaaactg ctatgtccta
     2041 aacgtttcga tacgctaagt atatttactc cttgataaag taaaatagat gcatgcgtat
     2101 gtcttagtcc atcaataaac agtcgaagag aaaataggga aaaattgatt actaaagaaa
     2161 aacttactag gtcgacggtg tagttgattt tcatacttca atagattcga attaaacgat
     2221 ttcgattggc ttataccagt cctatccaac gaaagatata tcgttcttaa ctctagcggt
     2281 ataacttgaa gctttacgta aagccaatgg gaatgaactg gagatgactt tttattattt
     2341 ttagtaatag ctgatgggtt aatacttaca actttccttt tcaaagtacc atataaaaaa
     2401 acggtggggt tgtttagcga aataaaggat ctaagccaat agcttagatc ctttattcta
     2461 tgctttatat
//
LOCUS       DASJTC010000180         2400 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.186, whole genome shotgun sequence.
ACCESSION   DASJTC010000180 DASJTC010000000
VERSION     DASJTC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.186"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            207..2069
                     /locus_tag="U4B19_002780"
     CDS             207..2069
                     /locus_tag="U4B19_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2078434.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="U4B19_002781"
     CDS             2069..>2400
                     /locus_tag="U4B19_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2078435.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DASJTC010000181         2350 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.187, whole genome shotgun sequence.
ACCESSION   DASJTC010000181 DASJTC010000000
VERSION     DASJTC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.187"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..629
                     /locus_tag="U4B19_002782"
     CDS             <1..629
                     /locus_tag="U4B19_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEN2078436.1"
                     /translation="IPSTEEMADILCRIRKMNYPIKEALEDHGRMGILLVDPEENELE
                     IYHEENKNDQREIPETMDQEALLRKSEEKFSKLSTGSYFEKIHLNVTNLTKERQFLNH
                     VLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDLPTDEVIGLDFLKFMIDTESMEGL
                     IAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSEPKEKNKTDIRCDA"
     gene            838..1863
                     /locus_tag="U4B19_002783"
     CDS             838..1863
                     /locus_tag="U4B19_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEN2078437.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            1902..>2350
                     /locus_tag="U4B19_002784"
     CDS             1902..>2350
                     /locus_tag="U4B19_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HEN2078438.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWD"
BASE COUNT          805 a          383 c          505 g          657 t
ORIGIN      
        1 ttatccctag cacagaagaa atggcagata tcctttgtcg tatccgtaaa atgaattatc
       61 caatcaaaga agctttagaa gatcatggac gtatggggat tttgctcgta gacccagaag
      121 agaatgaatt ggaaatttac catgaagaga ataagaacga ccaaagagaa attccagaaa
      181 caatggatca agaagcatta ttaagaaaat ctgaagaaaa attcagtaaa ctatcaacag
      241 gttcttattt tgaaaaaatc catttaaatg ttactaactt aacaaaagaa cgccaatttc
      301 ttaatcatgt acttggtttg aaggtacata atgaagaaga gggcatccat gtattgaatg
      361 aaggagattt ccatgttggt ttgacagaag ctagcggagg cgcaattgat ttgcctacag
      421 atgaagtgat tggacttgat tttctgaaat tcatgatcga tactgaaagc atggaaggac
      481 tgattgctca cttgcagcaa ctatcagttg atttcttctt agacaaaaag cacacagtac
      541 tcacaatcta tgatgctata ggaatcgaat ggtggttcgt cagtgagcca aaagaaaaga
      601 ataaaactga cattcgatgc gacgcttaac tttttctttc ttaacgattg tcgcatgtct
      661 cgtcatttta tgaacggtca tactatcgct tattctagag atagaaagag aaaatagtat
      721 accgggggaa actgcgcggt ttttcgcgca gtttttttgt gtaaaaaagg ttgattcctc
      781 atagattaag ctttaagctt ggactgtaag aatctgatag aaaagcagga gaaaaagatg
      841 tatccacaca cgaaacagct tattttgaaa aagatggaag aaggatttta tccaggtgta
      901 gcagtcgcct tcataaaaga aaatcaagta gaaacattta cagccggctt tgcccaagtc
      961 gttcctgaaa agcagctgat gacagaagat ctgttgtttg atgcagcctc actgacgaaa
     1021 gtcgttgcaa ccacttcatt agttttacgt ctattagaag atggaaagat agaactcgat
     1081 caaccgcttc ataaatatct tcctgaattt gaaaatcaga caatcacttt gcgtcattta
     1141 ctgacacata cttctgatat caaaacatgg ataccaaaca gagataagct atctcaggaa
     1201 gaattgatcg ccgcttattt gaagctagaa ccaggtgagc aactaggaaa agaagtaaaa
     1261 tatacagatg ccggtacgat tcttcttggg ttgatgctag aggaaattta ccaagaaact
     1321 gcagtggaaa tctttcaaga acaagtattg gaaccattag gaatgatgaa cagcttattt
     1381 cttccttttc cttccaaaaa aatcgttccc acagaacaac tgccttcggg tgaggttttg
     1441 cgtggacaaa ctcatgatcc gaaagcaaga gttttggctg aacatgcagg taacgcgggt
     1501 ctctttattg atttgaaaga cagcattact tttgtccaaa tgtatttgaa caagggaaaa
     1561 atccgtaatg gtcgtttttt agaagaagag acgattgatt ctttactgca agatcaaaca
     1621 ccgaaaaaaa atggctatcg ttctttagga tgggacttaa aaaaggatgt agaagacggt
     1681 catgcgattc tttttcatac tggttataca ggtacgtttt tcttgattga tataaaaaca
     1741 caagaagcgt tgatattttt gtcaaatcgc gtacatcctg tcgatcatcg tgcggagtat
     1801 ctcatatgtc gagatgaaat tgttgaaaac tttttgaaag agaaagcggt attaccgaaa
     1861 taatgctata atagctatga aaaatgagag gagtttgaat tatgcaggaa cctatgtttt
     1921 taaaaccagt ttttcaagaa aaaatttggg gaggaagtcg tttacgttca gtctttggtt
     1981 ttgatatccc taacgataaa atcggagaag attgggcgat cagtgcgcat cctcatggtg
     2041 tcagtgtagt agaaaatggc gaattcaaag ggaaaagatt agatgaacta tggtcagaac
     2101 acaaggaatt gtttggccat ccttcagaac cagtgtttcc attattgatc aagatcttag
     2161 acgcagaaga tgatctatct gtacaagtac atccagatga tgcctatggg atgaaacatg
     2221 aaggtgaatt aggaaaaact gaatgctggt atatcatcga tgctgaacct ggtgcagaaa
     2281 tcatctatgg tcatcatgca aagacaagag aagaactagc tgagatgatc aaagatgggc
     2341 gctgggatga
//
LOCUS       DASJTC010000182         2321 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.188, whole genome shotgun sequence.
ACCESSION   DASJTC010000182 DASJTC010000000
VERSION     DASJTC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.188"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            21..2174
                     /locus_tag="U4B19_002785"
     CDS             21..2174
                     /locus_tag="U4B19_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEN2078439.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          797 a          414 c          519 g          591 t
ORIGIN      
        1 aataggcgga aggataggaa atgatgaaag attttatgaa aaaaatatta aatacttctt
       61 ggttagaaaa aataagaaaa ggtgcacaag aacctaagaa caaaacgttt cgccggtccc
      121 atgttccttt tcgcttaaat tttctttttt tcattatatt tacgttattt gttgcgttga
      181 ttgctagatt gggttatttg cagatcgtga acggcgaaga gatggaggcg cgagtgaagt
      241 ctacttctac gattacggtc caagaaagta ctcctcgcgg aatgatctac gactccacag
      301 gaaaagcttt agttacgaat aaagcgaatc aggccatcac atatacgaga ggaacacaag
      361 ttacggctaa agatttgctt gaaatcgcaa cgaagctgaa ctcgctgatc gatgttccgg
      421 tagatgaaaa tctgacagat agagataaaa aagatttttg gctggctgat cccaaacatt
      481 tgaaagaagc cacaaaaaga ctttcggcaa aagaaaagca attagggaac agcgaacaat
      541 atagtgcgat ggtcaataaa gtcacagctg atgagattaa tttaaacgag gagcaattaa
      601 aaattgctac catctttaaa cggatgaata gtgcatatga attaaacact gtctatatca
      661 aaaactctgg tgttactgat caagagttag ctgttgtggc agaacgcgct tctgaacttc
      721 caggagtttc gactggcaca gactggacac gagaatataa tgcagcaagt tcactgaaaa
      781 gtattcttgg cagtgtgacg actgaaaagc aaggacttcc agcagatgaa gcagagaaat
      841 acctagcaaa aggttattct cggaatgacc gtgtaggaca aagttatttg gaaaaacaat
      901 atgaagatgt cctgcaagga accaaaaccc aatatgaagt ttcccttgat aatgaaggaa
      961 atgtttcgaa tcaaaaagaa atcttctctg gggaaaaagg ttctaattta atgttatcta
     1021 tgaatgcaga attccaatca aaagtagaag aaattttgaa aaggaattat caaacattga
     1081 tcaacaacgg caaagcacag tattcgcctg gtgcttatgc agtggctatg aatcctcaga
     1141 caggagaagt gctagcaatg actggtttca gtcatgaaca aggttccaaa gaaatcaccg
     1201 agaatgcttt aggcacgatt actagcgcct ttgatccagg gtctgtagtc aaggctggta
     1261 ctttgacagc aggctgggca agtaacgcga tcagcggaaa tcaagttttg attgatgagc
     1321 cgattcgtct gcaaggtgct tcagaaaaaa gctctgtctt taatcgaagc ggacaagttg
     1381 ctcttgatgc agtcaaagca tcggaattgt cttctaatac gtatatgatc aaagtggcgt
     1441 taaagatgtt aggactagac tatacacctg gaatgggact acctagttta gatgaagaag
     1501 ctaaagctta tcagcaattg agagactctt tcaaagagtt tggtcttggt acaacgactg
     1561 ggatcgattt gccgaatgaa tctcctggga tctcacgttc tgttgattat atgaagaagt
     1621 tcaatgcgga taatggcaaa gaatggtatg ctccaggtaa ctttaccgat ttggcatttg
     1681 gacagtttga cacatataca ccaatccaat tagctcaata tgcttcaaca gtcgctaatg
     1741 gcgggaaacg agtacagccg cacctagtca aagcgatcta tggcaatgac gaaaatggaa
     1801 acttgggtga agtgaagaaa gaaattggca caacagtaga aaatacggtc aatatttctg
     1861 cagaaaatat ggcgatcctc cgtgaaggat tccaccaagt tgtccatggt accgatcctt
     1921 acacaacagc caaaccatta gccagtgcga aaatggacct ttccgcaaaa acagggactg
     1981 cagaaacggt tgcggaaggt catccagata ttgcgacagt caatagtaat atcgtggcat
     2041 acggaccaac agacaatccg gaaattgcca tcagtgttgt cttgccgaac ttattagacg
     2101 aacaagacca cataaacttg acgattgcta aagaaataat ggacgcgtat tatgatatgt
     2161 tcatggcaaa ataaaaaaga ggtcgtgaaa aagccgctta gtttcgagta tgaagaaaaa
     2221 ttttgtgaaa atagcttctc acatttttgc ttaaatttga cttcataccg agaaactggc
     2281 ttttgaacac cgtttactcg gcaaaaaagg ttgtgacaaa a
//
LOCUS       DASJTC010000183         2318 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.189, whole genome shotgun sequence.
ACCESSION   DASJTC010000183 DASJTC010000000
VERSION     DASJTC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.189"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(106..552)
                     /locus_tag="U4B19_002786"
     CDS             complement(106..552)
                     /locus_tag="U4B19_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEN2078440.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(579..755)
                     /locus_tag="U4B19_002787"
     CDS             complement(579..755)
                     /locus_tag="U4B19_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEN2078441.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(917..1345)
                     /locus_tag="U4B19_002788"
     CDS             complement(917..1345)
                     /locus_tag="U4B19_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEN2078442.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGKIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            1437..2297
                     /locus_tag="U4B19_002789"
     CDS             1437..2297
                     /locus_tag="U4B19_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEN2078443.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
BASE COUNT          686 a          455 c          378 g          799 t
ORIGIN      
        1 agctagaaga aagaataaga ttctttcttc tagctgtttt ttagttcttt ttttacgaac
       61 acaaaaaact gcggataatc actgtccgca gtttaaatga taaaattaag attgtaatag
      121 ttctttcaca atcgcattca cttggtttcc atctgctttt cctttgactt taggcataat
      181 tgcacccata actttgccaa attctttcat tgatgaagca ccagtttgag tgattgcttc
      241 ttgaacaagt tgtcgaattt cttcgtcgga aagttgttcc ggcatatatt tttcgactat
      301 tacaatttca ctttttactt tttcagctaa atcgtcacgt cctgcttctt caaactcatg
      361 caaagaatca cgtcgctgtt tcatttcacg agacaacacc gttagttctt cttcttctgt
      421 caagtcgtga ccttctttaa tctgctcgtt ttgtatagat gacttgatca ttcggatgac
      481 ttgtaaagat tccttatctt tcgctttcat cgctgctttc atgtcatcgt ttaatctact
      541 tagtagtgac acactaccaa ctccaattca tcacagaact agaatttttt gcgttttcta
      601 gctgcttcag attttttctt gcgtcttaca cttggttttt cgtagaattc acgtttacga
      661 gattcttgta aagtacccgc ttttgacacg gaacgtttga agcgacgaag agcatcgtca
      721 agtgattcgt ttttgcgaac gactgttttt gacatgttaa ttccctccct ccgagcttaa
      781 aaagtaaccg taattgttat tgaaatatat ctcaaaaaac aagactgtcc tgtaaatatt
      841 atatctaatg ctgttttgac cgtcaaggaa atcttgcttt tttttaaaat tatcaaatgt
      901 tattttattt aaatccttat tgacaatttt cacatcttcc gaaaatctcg aatctatgcc
      961 cttcgatcgt gcatccacct agctgttctt cgaaataatt catcggacac atatggattt
     1021 ctttcgttgt tccgcacact gtacagataa aatgatggtg atggccgatt ttcccctgac
     1081 agcaatgaaa acgaaacttc atttctccat ttaaatcggt ttcttccagt aaatcgatct
     1141 ctgcaaaatc atggagattc cggtaaatcg tatcataact tacccctttg aattcctgat
     1201 tcatatagtc aaaaacttct ttagcagata cgtagcggtt acgcttcact aaataagtta
     1261 acaatgcttc tcttttttta gtatatttaa aaccgttatt tttcaatgtt tctaatgatt
     1321 gttcgatcaa tgtctgtttt ttcatgactt attcctccaa atcaaaatga ttacgaagta
     1381 cgcattctag agtatggtat aataaacata aaaaaaatgc aagtgaggtt ttatccatgt
     1441 cttccgattc tacagaaatc gttacttttt ttgtcatctc cgactctgct ggagaaactg
     1501 cgacaaaact agcgcaagct actatggctc agtatcctac cgttgagttc aacctattcc
     1561 gcagaacgtt cgtgacagat gaaaaaacat taaggcaggc attgcaagat gcattagaag
     1621 aaaaagcact tgtcctgcat acattaatca atcaagaact gatcgagata acacgttctt
     1681 tttgtgaaga acatcaatta tttagttttg atgtgatgac gccaccgata tcagaaattg
     1741 aacgtttaac tggtatcaag ccaattcggc aacctggtgc cctccattta ttgaatgaga
     1801 actatttcaa acggatcaaa gcaatggaat ttgctgtaaa atacgatgat gggaaagatc
     1861 ctcgtggttt tttagaagca gatgtcgttt tgctaggcgt atcccgaact tccaaaacgc
     1921 ctttgagctt gtttttagct aacaaaaatt taaaagttgc caatttacct ttgatcccac
     1981 aagcacatat tcccaaacag ctttgggaga tcgatcctaa aaaaatcgtg ggactaacga
     2041 ataacccaga tattttaaat aatatccgta aagaacggat gcgctcttac ggattaaacc
     2101 ctgatactgc ttattccgat atcgaaaaaa ttagagctga acttgatttt gcgaatgatc
     2161 tttatgaaaa actaggatgt gtagtgatca atgtggcatc tttatctatt gaagaaacgg
     2221 cttcgcttat tttgaatgct ttagacctag aagatcatag ttattatgga actgaaacaa
     2281 cagaaaaaag cgattaatca tttttgtctt tttcttaa
//
LOCUS       DASJTC010000184         2239 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.190, whole genome shotgun sequence.
ACCESSION   DASJTC010000184 DASJTC010000000
VERSION     DASJTC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.190"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(202..1407)
                     /locus_tag="U4B19_002790"
     CDS             complement(202..1407)
                     /locus_tag="U4B19_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEN2078444.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            2101..>2239
                     /locus_tag="U4B19_002791"
     CDS             2101..>2239
                     /locus_tag="U4B19_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2078445.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PY"
BASE COUNT          800 a          370 c          263 g          806 t
ORIGIN      
        1 tcttcacata aactttttac tttattaatt actactttgt catattcatt taacacacta
       61 ttattaccat tacttagacg ctcagattct acgaaacaac tcaaatcaga aatttcccaa
      121 aaaaattttt tgttaaaatc caatttcctc aactcctcca aactacgtaa atgatatagt
      181 ctcataataa aataaaacct ctcagctaat gttgttgaaa ttgtccacta aacctaattg
      241 atattgctta ggagatacac ctgcgtactt tttgaatact ctcgaaaagt gtgaagaatc
      301 tgaaaatcct aaacctaaag aaacttcact gatagtatgg tttgatttca acattatttt
      361 agccgtagaa atttttcgct tagagatata attataaatc gtaatatgca tttcattctt
      421 aaagctttct cttaatttag tttcacttac agaaaaataa ttagctattt cctttacagt
      481 aattctttta tagatattta agccgatgaa ttgaacgaca gaagaaatta agggagaaag
      541 atgctctatc ttagtttttc cgatttcctt agtataataa acaatcccag aatccctcac
      601 ttttaagacc tcaggtaacg atacagctaa ttcgttttct tgtatcaaag catctctact
      661 ttgatacgct tctattatat ccattcctaa tgttatagac gtttgtgcta acttttcaaa
      721 aacaatgata gaataatttt tttcagaccg aatcgtatct ccactaacac ttggaattat
      781 tccattactc agattaaaaa ccatgttttc taaatttcga atatctccat ttttaacata
      841 ttccagtatt ttattttcat aaagaaacag atatatttct ggatcgtaat tttgtgataa
      901 cagattatct attttgattt gctcgatagt tttacttaga ttttctgtat attcaattaa
      961 cggttcatgg aatagatctt ctatttttcc agtaaaaaaa taatgaacta aaatcagaat
     1021 atctcgaatg tctcctaaag aatacaaagt caaattttgc aatgaattat agagtcgttc
     1081 tctatcagcc attgcaacat tcaaattgtt catttttagt ttaaaaaaaa ctttatcaat
     1141 attatttgct ctaaatggac caaataagta atatctggtt ttaaacatgt atagaagaaa
     1201 catctcatta aaatcaccag taaagaaatg gaaatcttta gtttttttaa tattatcagt
     1261 tagtaacttt ttataatcat aaaaaaaata agtagttcta tctgagcgat actcttttat
     1321 aatactgaat tcggaattaa aaaccacgat aggtaacttt aaacaaacat gaagagactt
     1381 taataagact tctttatctg aaaacacttt tgtttctctc tttcctttct acatcattaa
     1441 atagtatttt atacgttatc taaaatacta ttcctctgtc aaaataagct aacttacttc
     1501 attataaaat atccacttat cgctttcatg tatattccac cgtaaaaagt acattttatc
     1561 accgagaact catcattttt catcatgatg aatactattt acatatatct agttcaaaga
     1621 caataattat tatgatccgt tattatctaa gcactataca gataatagga atttttcgtt
     1681 tctaaatatt ccaatttggg aatccaataa aaattttcgt tcaaatacgt gtctattcga
     1741 actcttttct agttgttaat tatgaagggg gaagaaataa aaaatacaat ctatacacga
     1801 ataagaatat tatccaaaag gaggttttag aaaagattta ttatacgtaa aaagctcaag
     1861 acattttctt agccataagg acatgtttga tatttaagaa gatatatgaa ttttcttttc
     1921 taagtataaa gataactatc cattatcaac taaaaaaaca tttttgaaaa taaacatcag
     1981 cagtcctatc aatgcatgtg agcttataga agttattcgc agcgcagcat aattgcatta
     2041 gcttcttttc gtgttttttg cctcttttcc ctttaaatct taatggagga ggtgtgcaat
     2101 atgtcagata acgacgaacg cgtaaatgcc cagttgattc gattctttga aaaggtcatt
     2161 ttgaacaccg ctaatactta ttataaaaaa caagaagaaa ttcaaaaaca tgaacaactt
     2221 gatcaactcc caccatatt
//
LOCUS       DASJTC010000185         2118 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.191, whole genome shotgun sequence.
ACCESSION   DASJTC010000185 DASJTC010000000
VERSION     DASJTC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.191"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..426)
                     /locus_tag="U4B19_002792"
     CDS             complement(<1..426)
                     /locus_tag="U4B19_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEN2078446.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="U4B19_002793"
     CDS             complement(535..1566)
                     /locus_tag="U4B19_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEN2078447.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          665 a          339 c          362 g          752 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggtagg agcaattata ttgagaatta atcctttttt atataagttg tttgcttatt
     2041 gatggattgg ataaattaaa ccatcacaac gagtggaaat tatatgaaat ttttcataaa
     2101 aattcatata gaaacaaa
//
LOCUS       DASJTC010000186         2071 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.192, whole genome shotgun sequence.
ACCESSION   DASJTC010000186 DASJTC010000000
VERSION     DASJTC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.192"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            684..1016
                     /locus_tag="U4B19_002794"
     CDS             684..1016
                     /locus_tag="U4B19_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEN2078448.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1635..1976
                     /locus_tag="U4B19_002795"
     CDS             1635..1976
                     /locus_tag="U4B19_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEN2078449.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          722 a          278 c          434 g          637 t
ORIGIN      
        1 cacaaacgta gccttttacg tcataagtgt taaaagaaat aattaatttc aaatatagaa
       61 aagggaacgt atgtttgcat actccttttt tttgagttat aataatacgc gaagagagat
      121 aatttcatta tttaaacaag ctaaaaatct tgatattact tcttttcagg caatacgaat
      181 attattcgct tacatttttt gtataaattt aattgtctgc tatgcttgtg atgaattgat
      241 tttcgttttg cacttttgga tgataaattt ggacaattga aaatacaaaa aaaagaaagg
      301 cggtatgaac tcggaaggct ggtaaccttc gtcagcgttc atacacggca ttaagatttg
      361 gcccttaatg cgttaacctt tcagtgttac ggtattaccc gcaatggatt tagtataaca
      421 gatattcaaa agtgatacca ctttttttgg ctgtgttaag gttaaaaata cgtgatatta
      481 cgatgaatct atgcctaagc ataatgttat aaaaatgtcc gtgtggctct ttgccgtatg
      541 acgaacgctg aaattagagg gaacgcatgt ttggatgcgt tccctctttt ttgtttgtca
      601 tgaacgaaaa tcaattctct atttttgaaa aataacaaga aaaacttgtt aaaaatattt
      661 aaagaaattg ggtggtttat tttatgtcat atacaggaat tttgagtctc aaagatattt
      721 gtcactacgg taaacggtgt accgctacag agaaaatcac gaagaaacta tcaactggcc
      781 aaagtaaaac cgttgtgcag tgtaagaaat atataatcca gaaagacaaa gtctcagagg
      841 aaatgattta ttacattggg aaacaaaagc aaataatttt aaaagatcct atccccttaa
      901 aagagctata tccaacaatc aaacatgttt atgaccaaaa cggtgtactg attggtcgaa
      961 gaaaaaatgg agtcttgcgt tgtaccgcaa aagggatggg gcgtttgatc ggctgaattg
     1021 atcattctca taacttgtat tttatcgttt ttcgcttgct tgaaaatatt gtcgagactt
     1081 ctaaaggaac ttagagtcac tagaaaaata aggagtagac ttgatctatc aacaattccc
     1141 ttcggggagt gacggggtag gttttaacga ttggtaacgt aaaaaattgg tcaatcgagt
     1201 aaatgcgctt gtttggatag tggtgaaatg aatttgtgta aataataagc gcagagtaaa
     1261 caagagggaa tttagaagtt agacaagtta gaatagcgtc tagattaatc atgagcagct
     1321 actaagggat ctagactttg acgcaaactc atggaataag gagtaggtct tctcgtaatg
     1381 gggaggacgc acaagaaatt gtgtcgtgtg ctgcagtaca tgtagatact tgcgaggacg
     1441 ttgattattg gatcaatcga gcatttaccg ctgtctacta gggtttctcg tttcggacgc
     1501 cataaacgag tcgaaatttt ttatgtattt ttaagtaaac gaaaaacgat aaaatatgaa
     1561 caagtcctga ataaagtgaa aaaattgata taaaacatag agaaaatgaa atttacgtaa
     1621 tggaggaatg acaaatgaaa aattatgatc cgaatatacg attgggcaca catactatta
     1681 aggtttcttt ccaacgttgg gattacaaag gatttgtgac gtttagaagg ggcggaaatt
     1741 gtaaaggatt agatgtttta gcattagacg aggatgattt atacgatcaa acattgactg
     1801 ataatccaat tggattcggc ttgctacctg aagatgatga aggtaatgaa tggtttaaaa
     1861 tgacgcttat gaatgataat ggagacgaat tatctgttga agatacttgg tcatatttga
     1921 gtgattatat tgtcagcttt gaaatcattg aatttgtagc ggataaagaa gaataaaaac
     1981 tggaggaata aattttacag ataaaggctt agaaggagtc acgacgatta ataaaaaggt
     2041 agtattacct taggcattga gtaaggaatt t
//
LOCUS       DASJTC010000187         2047 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.193, whole genome shotgun sequence.
ACCESSION   DASJTC010000187 DASJTC010000000
VERSION     DASJTC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.193"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..379
                     /locus_tag="U4B19_002796"
     CDS             <1..379
                     /locus_tag="U4B19_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEN2078450.1"
                     /translation="IVADNKVPGDPMSVMALTIAKPADCKLDIVKISDVKGIMETSPN
                     DNYMILIKGPKEAFELVQNLPEIKEINFGGVSKKSESKQYGKAVFLTPEELEATKKIL
                     DQEVKIFVQQVPSSSIENVDFSK"
     gene            393..1142
                     /locus_tag="U4B19_002797"
     CDS             393..1142
                     /locus_tag="U4B19_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEN2078451.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1142..1972
                     /locus_tag="U4B19_002798"
     CDS             1142..1972
                     /locus_tag="U4B19_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEN2078452.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT          646 a          276 c          412 g          713 t
ORIGIN      
        1 tattgtggct gataacaaag ttccaggaga tccaatgtct gtgatggctt tgacaattgc
       61 taaacctgct gattgcaaat tagacattgt taaaattagt gatgttaaag ggattatgga
      121 aacatcccca aatgataatt acatgatttt aattaaagga cctaaggaag cttttgaatt
      181 agttcaaaat ttaccagaaa ttaaagaaat taattttggc ggtgtgtcta agaaaagcga
      241 gtccaaacaa tatggaaagg cagtcttttt aactcctgaa gaattagaag ccacaaaaaa
      301 aattcttgat caagaagtga aaatatttgt gcaacaagtc ccatcttcaa gtattgaaaa
      361 tgtagatttt agtaagtaag gggggaaaat aaatgttatt aaaagccatt ttattaggtt
      421 tagttggtgt ggtagctgtt attgactcca ggttaattgg tcgacaaaat attggtagac
      481 cactaatttt gagtacactt gccggacttg tgctaggtga cttgcataca gggattgttc
      541 ttggggcatc tttagaatta atttctatgg gattcgtctc aattggggcg gctggacctc
      601 ctaatatgca acttggtagt attattgcca ctgcttttgc aattttaact aatagtagta
      661 cagaaacagc cttaacaata gctatcccag tggctgtagc tggtgaattt atgagtatta
      721 tcatgcgcat gataattgct caatttggac atgctgcaga taaagccatt gaaaataata
      781 aatttagaaa agctcaaatt attcatattt attggtcatt tatttttaat gcttttgttt
      841 actttattcc aatcttttta acagtatatt ttggtgctga cgttgtggca gatttagtag
      901 ataaaattcc agaaatgatt acagacgcct tgactgtctc tggaaatatg ctttccgcat
      961 tagggtttgc aatgctatta agcacaatgt tgagtaaaaa attatatcct tactttattt
     1021 ttggattctt tattgttgcc tattcaggtc tttcactaat tggcgtgaca atttttgcag
     1081 ctctgattgc ctttgtaatg gatcaagtta aatatggaaa aagagaagag gcgcatggtt
     1141 aatggaaaat agtacagaaa aaaaacgttt aatcacaaaa aaagatctta ataagatgtg
     1201 tcttcgttca tggacaatca tgtgttcgtg gaattatgaa agacaaatgc atatgggatt
     1261 tatgtatggg atggcgccaa ttttaaataa agtttataaa aatgatgaag agaaaaaaag
     1321 agaagcttat actcgtcata tggcattttt taactgtacg cctcaaatga cttcatttat
     1381 tatgggactt tgtgcttcta tggaagaaca aaatgctcaa gcagaagaag gaaactttaa
     1441 tactgaatct atctccatga ttaaaaccag tttaatgggt ccttttgctg gaattggtga
     1501 ttcatttttc caaggtactt taagaattat tacatttggg attggattgt cttttgctaa
     1561 gcaaggtagt attttaggac cgattttagc tgttggcctt tttgctatac cttctatttt
     1621 agctgttttt tacggcggta aattaggata tgagtctggc aataaatacc ttactaaatt
     1681 gtatcaagaa ggtcttatgg atcgagtaat gcaatttgct tctatcattg gtttggctgt
     1741 agtaggtgga atggtggcta gcatggtgcc aattgtcacc cctttaaaat tcactacagg
     1801 gggaacagaa cttgttttac aagacttact tgattccatt attcctggat tattaccact
     1861 tgtatgtact ttgggaatat ataaattgat taagaaaaat gttagtacaa atatgctctt
     1921 attaggttgt attttggcag ggtttgtttt aagtataata ggtatcttat aatgattaag
     1981 gctctaattt ttgatatgga cggagtttta attgatagcg agattatgta tcaaaagtgg
     2041 gtattta
//
LOCUS       DASJTC010000188         1944 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.194, whole genome shotgun sequence.
ACCESSION   DASJTC010000188 DASJTC010000000
VERSION     DASJTC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.194"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="U4B19_002799"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="U4B19_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEN2078453.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="U4B19_002800"
     CDS             853..1041
                     /locus_tag="U4B19_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEN2078454.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="U4B19_002801"
     CDS             1054..1611
                     /locus_tag="U4B19_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEN2078455.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="U4B19_002802"
     CDS             1643..1885
                     /locus_tag="U4B19_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEN2078456.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          716 a          301 c          400 g          527 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acac
//
LOCUS       DASJTC010000189         1890 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.195, whole genome shotgun sequence.
ACCESSION   DASJTC010000189 DASJTC010000000
VERSION     DASJTC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.195"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            53..>1890
                     /locus_tag="U4B19_002803"
     CDS             53..>1890
                     /locus_tag="U4B19_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2078457.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT          704 a          232 c          380 g          574 t
ORIGIN      
        1 ggacgaaaaa tattcattca tttgaaataa tgcctaggtg gtggagaaaa gtatgaaacc
       61 aagaatatat agtcctactg aaacagattt ctcaacgaat ggcttaggaa ttttaaagga
      121 ttgtacaaga tgtgaaatat atgaagtagc taatggaaaa tatgaattag agttggatta
      181 tcctttagga actagatttg atgaatattt tgaaaatgac tatcaaataa aagcaaagcc
      241 aaacgatcaa gaagagtatc atatcttttt tattgatgat aaagatatag atactttttt
      301 aaatacagta actatttatg ctcagagtcg tacaaatcga cttggaagac gggtagtcac
      361 tcttgtggaa gtggactcta aaactggtca agaagcgata tcaattattg aaactaaaat
      421 ggataaaaaa tctgacatac gactttattc tgatattacg gctgtttcta gcacaatctt
      481 tgaagcgaga aatgttttaa attgtattgc tggtgaacaa ggatcattgc ttcaatattg
      541 gggcggagaa attaaacgtg aaccatttaa gctttctttg ttaaagcgaa gaggtcgcaa
      601 taatgttgga acgattcggt atggaaaaga tatgtctggt ttgaaggtca aattagattg
      661 gacaggtata aaaacaagaa ttattccata tgctgatcct caaagtgaag taggtacgac
      721 taatcgaatt tatggttcgc cagtagatag tgcctatatt aataactatc ctgatgtcta
      781 tacagaacat gttcagttta cagaagaaca aggagtaaaa gacattaaga gcttaaataa
      841 aatagctaaa aattacttta aaacgatcaa tcctggctgt gataagccta aagtttctat
      901 tacggttgaa tttgataagt tgactgatag tgaagaagcg aaagaatttg cgaagattag
      961 aaattatggt ctgtttgata catttaaaat atatcataaa aaatataata tttatcttga
     1021 atcaaaggtt agtggactac aatacgattc tttatctgaa aaaactttga aattagaagc
     1081 aggagacatt caagttgctt tttatcagca acaagctgta actattcagg ataaattaaa
     1141 agattatgca acgaataatt atatgagtga ttttaatgat tatgtttctt caatgattac
     1201 aggtcaaggc agtgcaggag gatatgtagt tttatggcca aaagaaaaac cttccaacat
     1261 ctttattatg gataatccag atttagaaaa ggcaagagaa gtactaagaa tgaataaaaa
     1321 tgggattgct ttttctaaga aaggatggcg ggggcctttt aattcagctt ggacattaga
     1381 tagtatattt aatgccaatt ttatacaaac tggattaatc aaagcagata tctttcaaaa
     1441 ttcttttaac aagactggag atgtattgaa attagttaat ggactactcc aaatttggaa
     1501 caacaaaaag aaaataatgg agttaactaa aaaaggaatg gaattttgga attctaaggg
     1561 ttcaattgga acgattggaa caactgattc tgttggtaat ccttttcctg gggcttctac
     1621 tccaacccct attgaagata attctttagt tattcgtaca aatggagacg gcaaatatat
     1681 tttgatttct cctaaagtag gaaaaggatt agttttatta ggaaatggta aagcaattta
     1741 ttttggagac ttagatgtac aaggaaaact tacagttaaa ggaaaagaaa taattcctgg
     1801 acaaggtggt ggtccatcgg gaggtggaga atctactggt gggtatccta gtgaattaaa
     1861 aacagatgca gaaaagagag cttggagaat
//
LOCUS       DASJTC010000190         1884 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.196, whole genome shotgun sequence.
ACCESSION   DASJTC010000190 DASJTC010000000
VERSION     DASJTC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.196"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(72..686)
                     /locus_tag="U4B19_002804"
     CDS             complement(72..686)
                     /locus_tag="U4B19_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_065828827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEN2078458.1"
                     /translation="MYEKFFKRLIDILFSILGLIMLWPLMTFVAILVRIKLGSPVIFK
                     QQRPGKHEKIFLMYKFRTMTDQRDEEGNLLSDDIRLTQFGKILRSTSLDELPELWNIL
                     KGDMSIIGPRPLLVEYLSLYSNEQRKRHFVRPGLTGLAQVSGRNAISWKEKFEKDIEY
                     VENISMMKDIKIFFLTLKKVIVRDGITSVASGTTEKFEGAKIDE"
     gene            complement(694..1776)
                     /locus_tag="U4B19_002805"
     CDS             complement(694..1776)
                     /locus_tag="U4B19_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037946024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEN2078459.1"
                     /translation="MSDEGYEQEYVKEAFDTNWVAPLGMNVNGFEEELAEYVGASHAA
                     ALSSGTAAIHLALKAAGVGKDDIVICQSLTFSATANPIIYQGAIPVFVDSDYETWNMN
                     PEHLRTALEKYPNAKAVLVVHLYGLSAELDEITKICKEHNVVLIEDAAESLGTTYKGK
                     YTGTFGDYGIYSFNGNKIITTSGGGMLVSNDKEKVEKVRFWATQSREKARHYQHNEIG
                     YNYRMSNIVAGIGRGQLKVLNERIEKKRYIHSFYKKELSKLSNIQFMPENNWNEPNYW
                     LSVITVKGINTNHLIDELDKNNIEARPIWKPMHLQPVFDKYEYIGTDVAQTLFEKGIC
                     LPSDSKMTDDDLYRVTNTIKRVMQNV"
BASE COUNT          563 a          386 c          251 g          684 t
ORIGIN      
        1 gaatttcctc taatatattc gttcgactga tataatcaaa aggatgaata agcagccact
       61 tttttttatt ctcactcatc tatcttcgca ccctcaaatt tctcagtagt cccacttgct
      121 actgatgtta ttccatcacg gacaataact ttctttagcg ttaaaaaaaa tatttttatg
      181 tctttcatca ttgaaatgtt ctccacatat tcaatatctt tttcaaattt ttctttccaa
      241 ctaattgcat tacgaccact gacttgagct aatccggtta aacctgggcg cacaaaatgt
      301 ctttttcgtt gttcattact gtatagtgaa agatactcaa ctaacagagg acgcggtcca
      361 ataatactca tatctccttt taaaatattc cataattctg gtagttcatc aagactagtc
      421 gaacgcaata ttttaccaaa ttgagtcaat ctgatatcgt ctgataataa attaccttct
      481 tcatctcttt gatctgtcat tgttcgaaat ttatacatca gaaaaatctt ttcatgtttt
      541 ccaggtcgtt gttgtttaaa gatcactggc gatccaagct ttattcgaac taggattgca
      601 acaaacgtca tcagtggcca taacattatt aaacctaaaa ttgaaaaaag aatatctatc
      661 aaacgtttaa agaacttttc gtagatacct ctattagaca ttttgcatca ctcgcttaat
      721 tgtattagtt actcgataca aatcatcatc ggtcatttta gaatctgaag gtaaacagat
      781 acctttttca aacaatgtct gagcaacatc tgtaccaata tactcatact tatcgaaaac
      841 tggctgtaaa tgcattggtt tccatatcgg gcgggcttct atattatttt tatctaactc
      901 atcaataaga tgattcgtat ttattccctt tactgttatg acacttaacc aataatttgg
      961 ttcattccaa ttattttctg gcatgaattg tatatttgac aatttagaca attctttttt
     1021 ataaaaacta tgaatatatc gttttttctc aattctttca tttaaaactt tcaattgtcc
     1081 tcgaccgata cctgcaacga tattgctcat tcgataatta tagccaattt cattgtgttg
     1141 ataatggcgt gccttctcac gagattgcgt agcccaaaac ctcacttttt ccaccttttc
     1201 tttgtcatta gacacaagca ttccgccacc tgaagtcgtg attatcttat ttccattaaa
     1261 agaataaatt ccatagtcgc cgaatgtacc agtatatttt cctttatatg ttgttcctaa
     1321 tgattctgca gcatcttcaa ttaaaacaac attatgttct ttgcatattt tagtaatttc
     1381 atctagttca gctgataaac cgtacaaatg cactactaat acagcttttg cgttaggata
     1441 tttttctagc gctgttcgca aatgttcagg gttcatattc caagtttcgt aatcactatc
     1501 aacaaatact ggaatagctc cttgatagat gattggattg gcagtagctg agaacgtcaa
     1561 agactgacaa atcactatat catctttacc gaccccagcc gcttttaaag ctagatgtat
     1621 agctgctgtt ccagaagata gtgcagctgc atgactagca ccgacatatt ctgctaattc
     1681 ctcttcaaaa ccattgacat tcatacccaa aggagccacc caatttgtat caaatgcttc
     1741 ttttacatac tcttgttcat atccttcatc actcatatga ggagatgcta ataatattcg
     1801 tttattttcc aacttaatcc catctttcta tgactttggt aggtacgcca acatttttag
     1861 tcttattatt aatatttttt atta
//
LOCUS       DASJTC010000191         1780 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.197, whole genome shotgun sequence.
ACCESSION   DASJTC010000191 DASJTC010000000
VERSION     DASJTC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.197"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            40..1533
                     /gene="guaA"
                     /locus_tag="U4B19_002806"
     CDS             40..1533
                     /gene="guaA"
                     /locus_tag="U4B19_002806"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEN2078460.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT          546 a          361 c          372 g          501 t
ORIGIN      
        1 aaaattatcg tacttgatta cggtagtcag tataaccaac tgatcactcg ccgtattcgt
       61 gagttcggtg ttttctcaga gttgatgagc caccgcatta ccgcagaaga agtaaaggca
      121 atgaacccta aaggaatcat cctttctggc ggaccaaaca gtgtttatga tgagaaggcc
      181 tttggtattg atgaaaagat ttttgatttg gggatcccag ttttaggaat ctgttacggt
      241 atgcagttga ttacttataa attaggcgga aaagttaaac cagccaaaaa ccgtgaatac
      301 ggaaaagcag aattggaaat cacttctgca gaagcggccc ttttccaaaa cactcctgaa
      361 aaacaaaccg tttggatgag tcatggtgac ctagttaccc aagcaccgac tgatttcgat
      421 gtcgttgcta ccagcaaaga ttgccccatc gcatcgatcc aaaatgcaga acgcaaaatg
      481 tacggaatcc aattccatgc agaagttcgc cattccgaat atggaaatga cttgttgcgc
      541 cattttacac tagatgtctg ccaatgtact ggtgactgga caatggataa cttcatcgac
      601 atggagatcg caaaaattcg tgaacaagtc ggtgacaaaa aagttttatt aggcctttca
      661 ggcggagtag attcctctgt tgttggtgtt cttttacaaa aagcgatcgg cgatcaatta
      721 acatgtatct tcgttgatca cgtattgttg cgtaaaggtg aagcagaaca agttatggaa
      781 agtcttggcg gaaaatttgg cttgaacatt ataaaagtcg atgccaaaga acgtttctta
      841 tcaaaacttg ctggcgtgtc tgatccagaa gaaaaacgta aaatcattgg aaatgaattt
      901 gtatatgtct tcgatgatga agcaaccaaa cttgccggtg aagaaggcgt ttctttcctt
      961 gcacaaggaa ccctttatac cgatgtgatc gaatcaggaa ctgaaactgc ccaaaccatc
     1021 aaatcacatc acaatgtagg tggattacct gaagacatgc aattcaagct gatcgagcca
     1081 ttgaacacat tgttcaaaga cgaagtacgt gaattaggta cccaattagg catgcctgac
     1141 agcatcgttt ggcgccaacc attccctgga ccaggcttag gtattcgtgt ccttggtgaa
     1201 ttgacagaag aaaaattaga aatcgtccgt gaatcagacg ctatcttgcg cgaagaaatc
     1261 gccaatgccg gtttagatcg cgacatctgg caatacttca ccgtcctacc aggcattcgc
     1321 tctgttgggg ttatgggaga tggccgtacg tatgactaca ctgtaggtat ccgtgctgtc
     1381 acttctattg acggcatgac cgctgacttt gcccgtatcc cttgggaagt cttgcaaaaa
     1441 atcagtgtcc gtatcgtaaa tgaagtcgca cacgttaacc gtatcgtgta tgatattacc
     1501 agcaaaccac ctgcaacagt ggagtgggaa taaagagcca acataatatg gatgaggaat
     1561 acctataaat caacattatt caattactaa agttcctgat tttcgtaaaa aataagatgg
     1621 tttcctacca ttttccctac caaagccctt gttagattaa ctactaacaa gggctttgct
     1681 tttattcact acctttattt tcctattgaa ttagcttttt atctaatcag atttttagta
     1741 ctataaatat atatatcaga tttttcgatt taaaagatct
//
LOCUS       DASJTC010000192         1699 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.198, whole genome shotgun sequence.
ACCESSION   DASJTC010000192 DASJTC010000000
VERSION     DASJTC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.198"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            81..1604
                     /locus_tag="U4B19_002807"
     CDS             81..1604
                     /locus_tag="U4B19_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEN2078461.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          620 a          273 c          348 g          458 t
ORIGIN      
        1 tagaaacata tgcagagtat tcaccggaac agatcaatcg tatgatcgat caaatggagt
       61 ctgtcaaaaa agaaagttat ttggacccta ctgtttttga agcagaggaa catatccaaa
      121 aagtcattca taaaatgttc cgtccagaca atgcctatac aaaatttgat tttcgaaatg
      181 gccgtccaac gaagtctgcg atcttaacca cgagttccat tgatatggcc aaaaagtatt
      241 atcgggccat caaagagatg acgaaagaat ctgattggtt aataaaagag tttcccaatc
      301 agcctatccg tgaaggacga acgatggagg atcctgattt cccacgaatc gctattacct
      361 attcattaga agaaaatagc cgagattcaa gtgcccaaca agaagaaatg caaaaaatta
      421 ttgaggaata taatcagtat tatgatactg cctggtcact agcagacatt gagcgttata
      481 acggagacat caataatcgt ttggcacgaa aaagagcgga attcaaacaa tttgggaaac
      541 aaatcgattt agtaatcgta gtggatcgtc tattgacagg ttttgacgcc cctacgattc
      601 aaaccctatt tgtggatcgt aatctagagt atgcaggatt gatccaagcc ttttcacgta
      661 cgaaccgtac gtatcctgaa aaaacaaaag gactaattgt tactttccga aagccagcga
      721 caatggagaa gaatgtcgag gatgcgacaa aactgtattc agaagctaaa gaagaatcgg
      781 gtttgattta tccaagttac caagaatcca aaaaaagatt caagcaagca tatggtaagt
      841 taaaagaggt ttcatccatg gaatccattg atgaacatac gccacttgaa atccgtatcg
      901 agtacgtcaa agcatttcaa gaattgaatc gtgcgtatga atcgttagtg acttatgatg
      961 attacaatga tgacatggtg caatcttcaa ctttgaacaa tcaaattcaa cttttggaag
     1021 aacaaattgg cgtgtacgag accgtgaagg gttcattgat ggaggaagaa ccagaaaaag
     1081 agggagaaga tttttcaacg atcgaatttt atagtgaaaa taccactaag ttgtatgata
     1141 ttgattctac ttatattgat caattactag gaacttatgc tgcgaatagt tcagatgcac
     1201 gagaagaaat tgaaaaagct ttggctaaat tgaataaaac agaaggagtc aaagaagttt
     1261 accgacaaat tttaaatgca tttgatgtgg ggactttaga tagtaatgaa gatatctttg
     1321 tcattaaacg tcgctatttt acacaagcaa gtgataattt gattcatgcc ttttccaatg
     1381 agtggtttgt ttctgagaaa gaattacatg cttctaatct gcaatatatg ccaggtgaag
     1441 atccaattcc taatatgaaa gcaattatca acagtaagga ttacgaagga tataaagcca
     1501 aacatccaga agcgaaacca tttaaatacc cacaagaaat gaagcgagca tggcgtaaaa
     1561 tgttagatga tgagttgatt ccacttgaaa atgaattgag gtaaacgaga agagttaaga
     1621 aaattgtacg attcaaagat agtagtaaag tggaagtgta gaaaataggg atagatgtct
     1681 ttttacttgt tcacatttt
//
LOCUS       DASJTC010000193         1690 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.199, whole genome shotgun sequence.
ACCESSION   DASJTC010000193 DASJTC010000000
VERSION     DASJTC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.199"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1454
                     /locus_tag="U4B19_002808"
     CDS             <1..1454
                     /locus_tag="U4B19_002808"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEN2078462.1"
                     /translation="LLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYA
                     ISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQ
                     RNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMA
                     QIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNL
                     ILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRY
                     GKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAV
                     IGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILET
                     YAERKDVEKYAHLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQ
                     VLEKELLEAISSLQTTPENEAWLQGALEVFKHE"
     gene            1447..>1690
                     /locus_tag="U4B19_002809"
     CDS             1447..>1690
                     /locus_tag="U4B19_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEN2078463.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
BASE COUNT          590 a          282 c          357 g          461 t
ORIGIN      
        1 ttcttttagc agatgaatcc ttagaggaat atgatacggt aagcaaacaa acgatgttgt
       61 atcgcgaatt actttcagat gaagaatcaa aagaagatct gattgcaaca atcgttgata
      121 ttttggggta tgcgatttcg ccagagtatc ttttcaacgt cttagcggat caagcaaaac
      181 aagcaacgtt ccaattgaat gatctaaaca aagcctttgt gcaattggca tctacctaca
      241 atcaatttaa tgggttattt gatgatgtgg atctccaatc aaaaaaacta ggaacagatg
      301 agcaacaacg aaatgtgacg attaccgaag tcattaagaa attgaatgac gtggaagttt
      361 taggtcacga tggggacgtg atcggggatg cttatgaatt tctgatcagt caatttgctt
      421 cagaagcagg gaagaaagcc ggagaatttt acacgcctca tatggtttct gatatgatgg
      481 cacaaatcgt gacgttggat caaaaagaac gacgattctt tagtgttttt gatccaacca
      541 tgggttcagg ttcattgatg ttaaatgtgc gtaactactt aactcaccca gataacgtta
      601 aatatcatgg gcaagaatta aatacaacga catacaatct agcaaaaatg aacttgattt
      661 tacatggggt agatgccgaa gaaatgaatc tccgcaatgg ggatacgttg aataaggatt
      721 ggccaacaga tgaaccttac acatttgatg cagtagtaat gaacccacct tattcggcta
      781 attggtcagc agatacgacc ttcttagacg attctcggtt taatcgttat ggaaaattag
      841 cgccaaaatc caaagcagat tttgcgtttc ttcttcatgg tttctatcat ttgaaggaaa
      901 cagggactat ggccatcgtc ttgccacacg gagttttatt ccgtggcgct gcagaagggg
      961 tcattcgtca aaaattacta gaagacggca gtatttatgc tgtgatcggt atgcctgcga
     1021 acttgttctt cgggacatcg attccaacga cggtgattgt attgaaaaag aaccgccaaa
     1081 ctcgagacgt attattcatt gacgctagtc gagaatttgt caaaggaaaa aatcagaata
     1141 aattatcgga agaaaacatt caaaaaatcc tcgaaaccta tgctgaaaga aaagatgtcg
     1201 agaaatacgc tcatttggca aactttgatg aaatcaaaga aaatgactat aacttaaata
     1261 ttccacgcta tgtagatacg tttgaagaag aagaaccaat tgatatggtt catgtcggaa
     1321 atgacatcaa gaagatacga caagaacagc aagtgctaga aaaagaactt ttagaagcta
     1381 tctcttccct gcaaacaaca cctgaaaatg aagcgtggtt acaaggtgcg ctggaggtgt
     1441 tcaaacatga gtaatgatat acaaccagaa attcgtttcc caggatttac ggaagcttgg
     1501 gaacagcgta agtttgaatg cttacttgat aaaaaagatg gagtaaggag gggaccattt
     1561 gggagtgcct taaagaaaga attctttgta tctaatagca attttgttgt ctatgaacaa
     1621 caaaatgcta tttatgataa ttacgaaaca agatataaaa ttactgaaaa aaaatataat
     1681 gaattaatca
//
LOCUS       DASJTC010000194         1682 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.200, whole genome shotgun sequence.
ACCESSION   DASJTC010000194 DASJTC010000000
VERSION     DASJTC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.200"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            200..742
                     /locus_tag="U4B19_002810"
     CDS             200..742
                     /locus_tag="U4B19_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078464.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1347
                     /locus_tag="U4B19_002811"
     CDS             898..1347
                     /locus_tag="U4B19_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEN2078465.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCGVMATIALDNEHPRI"
     gene            complement(1377..>1682)
                     /locus_tag="U4B19_002812"
     CDS             complement(1377..>1682)
                     /locus_tag="U4B19_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2078466.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          636 a          240 c          324 g          482 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgg aatggcaaca
     1261 ctttttttga caaaaattat aaggtgttgt gaatgctgcg gtgttatggc tacgattgcc
     1321 cttgacaatg agcatcctcg gatctgattt tctccggcag aagcacaatc attcaattac
     1381 gctgcctctc cagaggtatc agatccgcgc tcattatcaa ggattcgctt cgccaatctc
     1441 tgttgtcgaa tttggatcgg tgtttcatac gccaacgtgc catgtaagcg tagatagttc
     1501 caccaatgca cataatcaaa caattctaga cgtagctgtg ccagtgtctc aaattggtat
     1561 tgatgcacga attctacttt gacagattta taggttgatt ccacaacggc attatcatac
     1621 ggacaaccct tcctactcaa tgaacgagta attccaaaac cattcagaat ctcatgaatg
     1681 gt
//
LOCUS       DASJTC010000195         1664 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.201, whole genome shotgun sequence.
ACCESSION   DASJTC010000195 DASJTC010000000
VERSION     DASJTC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.201"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            131..505
                     /locus_tag="U4B19_002813"
     CDS             131..505
                     /locus_tag="U4B19_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEN2078467.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="U4B19_002814"
     CDS             complement(619..1248)
                     /locus_tag="U4B19_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEN2078468.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="U4B19_002815"
     tRNA            complement(1382..1454)
                     /locus_tag="U4B19_002815"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          283 g          557 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DASJTC010000196         1659 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.202, whole genome shotgun sequence.
ACCESSION   DASJTC010000196 DASJTC010000000
VERSION     DASJTC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.202"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            25..1092
                     /locus_tag="U4B19_002816"
     CDS             25..1092
                     /locus_tag="U4B19_002816"
                     /inference="COORDINATES: protein motif:HMM:NF026018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEN2078469.1"
                     /translation="MFVFQLIFTPILSVFQYIVRALGESKIFVMSGIIAAGINLLLLT
                     IFVVTMGMGILGLLLSIIISNVINLIFLIFRGQIFQYFATSSFDFLFLKSMLKYSIPL
                     TLNLTFGWLIVGFSRFYIGTYIGVTENGIFSFAMKFSSIILTLTSIVSMSMIEDAVIT
                     SENDDFIERFVEKNTYIFKMYIRLATLSIPIICCAYSFITNPDYKKSLVLVPILVIMA
                     CFSGMTTNIGNIFAVYNKTNQLLMISVVSGLLSVVGTYLLGYSFGVIGVSFATLLGTL
                     VLFGLRYYFGRKIAYYAFDWKRISLLLVLYSAIAFVCIHRGFYFNLFLTIIFSAIFVF
                     TYKQLIITYLKKFFRIKMAKR"
     gene            1322..1606
                     /locus_tag="U4B19_002817"
     CDS             1322..1606
                     /locus_tag="U4B19_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078470.1"
                     /translation="MSSVSESTSKIGRIKLGNNIFIGIYSIVFPGVSIGDNVIIRANT
                     LVNKDIPNNSVVAGSSCKVISTIEEHYEKNKNEFIKYRTSMILKQKNFFY"
BASE COUNT          525 a          207 c          297 g          630 t
ORIGIN      
        1 ttcccgaatt tagagtatta tcaattgttt gtttttcaat taattttcac tcctatccta
       61 agtgtttttc aatatattgt aagagcacta ggtgaatcaa agatttttgt gatgagtggt
      121 ataatcgctg cgggtatcaa cctgttactc cttactattt ttgttgttac aatgggtatg
      181 gggatacttg gtctcttact ctctatcata atttctaatg ttataaattt gatttttttg
      241 attttcaggg ggcagatctt tcaatatttc gcaacgagtt cttttgattt tttattttta
      301 aagtccatgc tgaaatactc tatcccactc actttgaatc tcacttttgg atggctaata
      361 gttggttttt caagatttta tattggaacg tacatcggag taacagaaaa tggtattttt
      421 tcttttgcga tgaaattttc atctataatt ttaactctta caagtattgt tagtatgtct
      481 atgatagagg atgcggttat cacgtctgag aatgatgatt tcatcgaaag attcgttgag
      541 aaaaatacct atatttttaa aatgtacatt aggttagcga ctctttctat tcctatcatt
      601 tgctgtgcat actcttttat aaccaaccct gattataaaa aatcattagt tctagttcca
      661 atattagtta ttatggcatg tttcagtggg atgacaacaa atatcggaaa tatatttgca
      721 gtatacaata aaacaaatca gttattaatg atttcagtag tgtcaggtct attgagtgtt
      781 gttggcacct atttattagg ctattctttt ggagtaattg gtgtctcatt tgctactttg
      841 ctcggtactt tagtattatt tggacttaga tattattttg gaaggaaaat tgcttattat
      901 gcatttgatt ggaagcgaat atcgctactt ttagttctct attccgcaat cgcttttgta
      961 tgcattcata gaggcttcta ttttaatctt ttcttaacga ttatttttag tgctattttc
     1021 gtttttacgt ataaacaatt aattataacg tatctaaaaa aatttttcag aataaaaatg
     1081 gctaaaaggt gagggtatac gaagtaagaa tagaatcttt cgaagaataa agagaaaact
     1141 atttggaaaa aatctgcgag aatgtattaa tgaaggtttg atattcggaa aaaaacatag
     1201 tttagtagag gttcctgaat ttgggagcga gccatattta attgaaatag gggataattt
     1261 cagatgttca tttggtgtta aatttataac acacgatggt ggagtctggg tcttaaggaa
     1321 gatgagttct gtgagcgagt caacttcaaa aataggtagg atcaaattgg gaaataatat
     1381 ttttattggt atttactcta ttgttttccc aggagtttca attggcgata atgtaattat
     1441 tagagcgaac actttagtaa ataaagatat cccaaataat agtgttgtag cgggtagttc
     1501 atgtaaagtg atttcaacta tagaggagca ttatgagaaa aataaaaatg aatttattaa
     1561 atatcgaacc tcgatgattt tgaagcaaaa aaattttttc tattaaataa tattgattga
     1621 ataaggaata tgttcaaaag gataacagct aaaatacta
//
LOCUS       DASJTC010000197         1644 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.203, whole genome shotgun sequence.
ACCESSION   DASJTC010000197 DASJTC010000000
VERSION     DASJTC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.203"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            165..587
                     /locus_tag="U4B19_002818"
     CDS             165..587
                     /locus_tag="U4B19_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078471.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            597..800
                     /locus_tag="U4B19_002819"
     CDS             597..800
                     /locus_tag="U4B19_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078472.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1011..1595
                     /locus_tag="U4B19_002820"
     CDS             1011..1595
                     /locus_tag="U4B19_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEN2078473.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          588 a          228 c          296 g          532 t
ORIGIN      
        1 tttttaaatg gcaatatttt ttgtaaataa atgtcaatca agaaaattta aacgattatg
       61 acattaaaaa tatattttat gaattttcta gtatataact tgatttatct gttaagattt
      121 attaagaaaa cgttttctta tttttgattt cgaggtgata gacaatgaaa aataagttta
      181 ttttatcttt aggtatttta actattggaa ttagtgtaac tggaactaca attcctacat
      241 ttgctgatag tacaaatgaa ccaactactg tagtagataa taaccaagaa ttaactcctt
      301 tagataggaa cggaatagcc attggtaacc caactaacga cttaactgtt actgaaaatt
      361 atttaatagt ttctccatta cttgctcgag cagcttctaa aacgatacga gtgacaggaa
      421 cgatgatttt tgataaagtt ggttggcctt ctagacctcc tcaatcacgg aattggaaag
      481 aaaaaagaaa tggttattgg tactatggaa aattgtattt acaaagttat aaatcagctg
      541 gaaacaattg gattgcgact tattcaggaa ccttaactag aaagtaggga ttttttatgc
      601 cagaatttgt gaacacacca aatttttatc aagatagcga tattctaagt aaaattaatt
      661 taccgaaaga agaatcagct ttacaagttc agcgaacttt acaggagaca caaaaaattc
      721 aaaaatcaat ttctaatcta gaagattctg aagaaaaaag aaaaaaaaga gaccaaaaaa
      781 catatcaaaa agaaaaataa tgattttaaa aaaagagtgt ctagtcgaca ttcttttttt
      841 ctaattgaaa gtgttcataa aatcacccat ttctggacac tttctttttt tgctttattt
      901 ttcaaaaaaa caattcattt catgttcata aacccataac aatatctatg ttcagttaaa
      961 ctatatggat aaagttatgt tacaataaaa ttaatgaagg agtgattttt ttgaaaatag
     1021 gatacgcccg ggtatctact ggtttacaaa atttggattt acaagaagac agcctaaata
     1081 aatttggttg tgaaaaaatt tttactgatc atatgagtgg ggcaaaaagt aatcgaccag
     1141 gattagaaat ggccattgat tttgttcgct ctggcgatac acttgtcgtt tggcgcttag
     1201 atcgtttagg tagaaatatg gaagatttga tttcaatcgt caatcgactg aatgagcgtg
     1261 gtgtgagttt tcatagtatt caagaaaata ttacgatgga caaatcaagt tctacaggac
     1321 agctcatgtt tcatttgttt gcggcatttg cagaatttga gcgtaatttg atcctggaac
     1381 gatccgcagc aggacgagca gctgctcgtg caagaggacg gtttggtggt cgtcctgaaa
     1441 aattgtctga acaagatttg gaattactga agactttagt ggatagcggt acacctatta
     1501 agactattgc ggaacggtgg aacgtctcaa gaaccaccat ttatcgttat ttggcaaaaa
     1561 tggaggagaa aaatggaaag acaaattctc attagttttt gtcatttaat gctgaaaatt
     1621 tttgccattt atctagaaaa gtga
//
LOCUS       DASJTC010000198         1602 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.204, whole genome shotgun sequence.
ACCESSION   DASJTC010000198 DASJTC010000000
VERSION     DASJTC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.204"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            51..257
                     /locus_tag="U4B19_002821"
     CDS             51..257
                     /locus_tag="U4B19_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078474.1"
                     /translation="MKQKVIGEVISKLENCVVICVDEYHDVDRNEILEKCGKVVAKYT
                     HVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(619..858)
                     /locus_tag="U4B19_002822"
     CDS             complement(619..858)
                     /locus_tag="U4B19_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078475.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            1144..1458
                     /locus_tag="U4B19_002823"
     CDS             1144..1458
                     /locus_tag="U4B19_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078476.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          549 a          219 c          280 g          554 t
ORIGIN      
        1 ataacgaaac tatcatttta ggaaaaaaat atatttgcca acctattggc ttgaaacaaa
       61 aagtgattgg agaagtaatc agtaaattag aaaattgtgt tgtgatctgc gtagacgaat
      121 atcatgacgt cgatcgcaat gaaatattag aaaaatgcgg aaaagtagtg gcaaaatata
      181 ctcacgttta tgagttggaa acaacagaat atctatataa ctatagcaac aaatatgaac
      241 atactttagt ttcataaaga tttaaataaa gtgccttcaa aagcgagaag accaagcttt
      301 tgaaggcact ttatttagtt ggcagtgatt gttcatgatt tttgatttat tcaaaaggtt
      361 cgcctagaga atctgtctct gtaacgaggc ctggtggaga aactcgataa aaacctatac
      421 tgccagaact gtcctatttt cggatagaca aactagtagt tttaaaaaga tcatccgatc
      481 caatcatttc aaaaaatgca aaactgatat caggatcatt tagctcaggc ttgttatcgt
      541 aaagcatttg taaaggttgc atttagttag gagtaaacgc gaaactatca tcattatttg
      601 ctgaagaaga tgaagaagct aattcagagt ttgtttgaaa taaaggagac gtgctgacag
      661 tttctttacc ctgttttttg cagaggtaga aacttttata tcggaagttt ctaccggaga
      721 atttttctca tttgtagaaa ttgttgaatc gatactattc gaaatttgcg tatctgaagg
      781 tatgttcttt gatgcagaag tcccacaagc agtaagtaac aataaatttg ataaactgat
      841 aaacagaatt cttttcatta tgtacacact ccttatagta tcgtttagca tacacctatg
      901 ctcagtatta ggcaaaatca atgcaaaact atcaagtctt ttatagtatc tattagtctt
      961 agtaatacat atatggagag ggtgatatcc cagttcaaac cagatacatt ggttaccatc
     1021 tgtattaacg attatgcctc taaacgcttt ttttatgaaa aaatttataa gtgaaaacgt
     1081 aggtatgata ttatctagag gtaatttttt tgggtattta aatagttgct aggaggatag
     1141 cttttggatt tttacagtaa ttttatctta ataatagcaa tattattact tctaaatatt
     1201 tggttttttg ataagagtag aaatgcaggg ataggtttta gaaccaaacg aagtacgtct
     1261 tctgaaaaaa agtgggtata ttcacagact attttttacg gaggggttat ttcaatcagt
     1321 ttactttctt caacacttta ttcttttaat gttatagacg tttccatgtc aaattttata
     1381 tctattattg gaatccttat ttctgcaatt attacacaac tacttcttgt gtttgaagag
     1441 aagtcaaaaa ataattaaag caattatgcc ttttagtaca ggaattatta cttcttacaa
     1501 atgtatagcg aatgtgaagc ctgctgtttt aattgttcta gaaaaacttt tgagtgaaca
     1561 attattttat gttaggatga attaatttct ctagagttta ta
//
LOCUS       DASJTC010000199         1573 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.205, whole genome shotgun sequence.
ACCESSION   DASJTC010000199 DASJTC010000000
VERSION     DASJTC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.205"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..637)
                     /locus_tag="U4B19_002824"
     CDS             complement(<1..637)
                     /locus_tag="U4B19_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEN2078477.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVL"
     gene            complement(648..1316)
                     /locus_tag="U4B19_002825"
     CDS             complement(648..1316)
                     /locus_tag="U4B19_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEN2078478.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1446..>1573)
                     /locus_tag="U4B19_002826"
     CDS             complement(1446..>1573)
                     /locus_tag="U4B19_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2078479.1"
                     /translation="IYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT          581 a          262 c          208 g          522 t
ORIGIN      
        1 atagaacttg atcaattaat tctttcaaat agtaaaaacg atgttcagct ataacaatag
       61 taaatccttt attttttaaa tcacaaagta agtcagcaag tttcttagtc gctttgaagt
      121 ccaaatttga agacggttca tccaatataa ttaatttaga gtttagcgat aaactacatc
      181 caatagaaat tttctgccgt tcaccacttg atagcgaaaa aatgttttta tctaatatat
      241 ttgaaagatc taataattca gctaattcat tgattctttt atccatatgc tcttttgtaa
      301 atccaaagtt ttccatcgaa aatactaact cactcgttgt atttgttgta aagaattgtc
      361 ctctaggatc ttgacttacg tagccaatat tttttgatat actttcttgt gttaaggatt
      421 gtagcgagtt attttcaaat aaaatttccc caaccaattt tccgtcataa agtcttggaa
      481 taagtccatt cattaacttt aatagtgtgg atttcccact cccagaagaa ccagtaatca
      541 atgtaagact gccttttttt attgaaaaat taaggtcaac aagcgcttgt tccttaacat
      601 ccttatatga aaaagaaaca ttgttaaatt caaacaattc tttcacctca aatcaaaata
      661 cttaagaaaa aaatagtaac ggaaaacagg atgatatagt ctaataagct catgtttgtt
      721 ttatagtaag aacttctttt cacaggagat tctatcccct ttgttattgc attagcggtc
      781 atttcttcag caatcatagt tgcacgtata agaacgggaa ctaacagaaa ttccaaatat
      841 tgcagaggct gaaaaaaaga caagcgaatg tttctcattt ttaaagattc tttaatcatt
      901 cttatttcag aacgataagt cggaaagaat ctgatcacta ctgtaatact taacattatt
      961 tttttcggta aatgtatttt ttctaaagag gataataatt cattgctagt atagacacta
     1021 accaacaacc atgagatcat tacgaccggc aacattcgaa atacaatata taaggatgtc
     1081 gcaaaaaata tagtaaaaaa ctctatcttg atagcatcaa taattttcat tactaaaaac
     1141 agaactgaat aaatcaagat gaaataaata catgtcttta ttttacgaaa catcaatact
     1201 aaaactgtag caataatgaa acaccccact tgaaatatat ttccctttcc taaaaaaata
     1261 atgaccggaa aaaaaatatt taaagtaatc aaggttaacg gattgatttt tgacactctt
     1321 tcacctccaa aaaataagat cgcactgtta tcttaagctg ataaaaatgc accgttattc
     1381 agataacagt gcgatcttat caaaaagcat aactgagaac aattctcaat gcaagctttt
     1441 tttaattaac tcagtaattc aatccaccca ggaacagaaa attgatacaa tcttctggtt
     1501 aatagcatag tcttttcttt tgaatagcta tgcttcaata tatcatagaa tgtagcgtag
     1561 aatgagtaaa tgt
//
LOCUS       DASJTC010000200         1561 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.206, whole genome shotgun sequence.
ACCESSION   DASJTC010000200 DASJTC010000000
VERSION     DASJTC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.206"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            50..1516
                     /locus_tag="U4B19_002827"
     CDS             50..1516
                     /locus_tag="U4B19_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEN2078480.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          434 a          290 c          329 g          508 t
ORIGIN      
        1 taaagatgta taatattttc tgaaaattta aagaattggg gtatagatta tggaaaaaca
       61 gcaagacaag ggcttcttcg ggcagccgaa gggattgtca actttgttct tcactgagat
      121 gtgggaacga tttagttatt acggtatgcg tgcattactt atctattata tgtacgatac
      181 agttgccaac gggggacttg gattaccacg agcaacagca ttagctatca tgtcgatcta
      241 cggatctttg atctacatgt ctagtatcgt cggcggatgg gtatctgacc gagtgttagg
      301 agcaaaacgc actgtattct tcggtggagt tttcattatg cttggccaca tcgtcttggc
      361 tttaccattt ggtgtaagta cattgtttgt ttcgatggca ttgattatta tcggaaccgc
      421 ctttttgaaa ccaaatgttt ctggcatggt cggacatctt tattcaaaga ctgacttgcg
      481 aagagatgca ggattttcga ttttttacat gggtgtcaac ttaggggcat tattagcacc
      541 actaatcgtt ggtacaatcg gtcaagaata taactatcat ttagggttct ctttagctgc
      601 tattgggatg ttctttggat tattacaata catcatccaa ggacgcaaga cattagacgg
      661 agtgggtaca acaccgccga atcctttgac aactgaagaa cgtaaaggat tcatccgtaa
      721 cttgatcatc gcacttgttg ttatcgctgc agtattcggc ggggcacaac taactggtca
      781 tctgacaatt gacttttttg tcaatacaat cagtgtattg gggattatct taccgttgta
      841 ttactttatc aaaatgctga cttcaaaaga tgtaactgct gaagaaaaac caagagtatg
      901 ggcttatatc ccattattcc ttgcagcaat cattttctgg tcattacaag aacaaggttc
      961 atctatctta gctttatttg ctagtgaacg tacacaatct acgctatttg gtgtaacgat
     1021 ccaagcaagc tggtatcaat cattgaatcc aattttcatc tttattctga caccagtatt
     1081 cgtaacgatg tggacgaaac ttggtgaacg tcaaccatct acagtagtga aattctctct
     1141 tggactttta tttgcaggat tatcgtactt gctattgatg ttgccgggta tgctttatgg
     1201 tacaagtgga aaagtaagtc cattgtggtt gttgggaagt ttcttcatcg tggttattgc
     1261 tgaattgtgt ctttcaccag ttggtttgtc tgtgacaaca aaattagcac cacgagcatt
     1321 tgaatcacaa acaatcgcta tttggttctt agctgatgct tcttcacaag caatcaatgc
     1381 acaaattgca cgcttctaca caccgggaac agaatctgct tacttcggta ttatcggtgc
     1441 agtagcaatc gtcggtggta tcttgctatt tactgtgaaa gaaccaatca aaaaactaat
     1501 gggctctatt cattaatcaa tgaacataaa aacaggaatt gcttatttgc agcagttcct
     1561 g
//
LOCUS       DASJTC010000201         1537 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.207, whole genome shotgun sequence.
ACCESSION   DASJTC010000201 DASJTC010000000
VERSION     DASJTC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.207"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..448)
                     /locus_tag="U4B19_002828"
     CDS             complement(<1..448)
                     /locus_tag="U4B19_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEN2078481.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLL"
     gene            complement(430..1419)
                     /locus_tag="U4B19_002829"
     CDS             complement(430..1419)
                     /locus_tag="U4B19_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEN2078482.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
BASE COUNT          504 a          281 c          197 g          555 t
ORIGIN      
        1 ttaaaagcga attaatatga accatacttt tttgagagtt attcagtaat tccttaaaat
       61 ctatttcttc tatagattta ccagctgact gggccattaa taactccagt aaaagaggta
      121 aattaatccc agcaataatt tgaattctat tgttgttcaa ctgtttaata gaaacattat
      181 taggtgttcc tccaattaaa tccgttaaaa taattagagg ttgcaaatga tgctgactaa
      241 ttatttcttt catactctcc tcaaaatcat ctggactagt attttcttta aatggaacag
      301 cactaatacc tgtggcgtca ttcattaaaa ttaattttgt tgcctttaaa taaccctcac
      361 aaagggtaga atgtgttgta atcacaatgt gaaataattg atttgccatc ttttgcaacc
      421 tcctattaat taatatttca tttgccacat atagcgtcta acatctaagg aatggcctct
      481 ttcaaaagca atcgcttcta ccatttggcg aataacaacg ccactaagta cagctgcaat
      541 atattctttc gctacactat ctatagattc taagtcaaaa tcagcttggt ctaaaaccat
      601 taatttatta ctgaattttt tagtgaagtc ttctacccgt tgatctaaaa atcttgtatc
      661 tccaatactt ttcactaaga tgaatggtac atcataatca gtgatttcaa aaggaccatg
      721 gaaaaattcc ccagaatgaa tagcattaga attaatccat tgcatttcca tgaaccagca
      781 agctgctgtg gagtaaattt ctccgtaatt gataccgcta ccaattgtgt aaataatttt
      841 atctcgtttt tggtttttac cccattcttt agcagttatt tcaaatttgc tcttttcagc
      901 aataacaaga tcatttaact tatcaaattc attcaagcaa gtttcccatt ttttagagcc
      961 atctaaaaca agtaaaatat taaataataa accataaaga attcctttat ttaaatcact
     1021 agcatcaaca tctttacccc aatcatagtg aacaggatat tcagctgctt cccatagagg
     1081 agatccttct tcattagata aacaaatagt cattgctcca aattctcttg ctttttttgt
     1141 tgcttctaca gtttctggtg ttgtgccaga atgggaacaa gtgataacta aagaattttc
     1201 agataatcct tttggtatag catagttaaa ttcgttagca gtataaacat gacaaggtac
     1261 agttaattct ttatcaaata gatattgacc agccattaaa acggcttgtg aacccccaca
     1321 tgctgtaaaa tatacatgat caattgtctt ttcctttaac gctgccattg ctgtttcaat
     1381 ctgttctttg tgtagttgtc tcatttcttt atccctcact ctaataagta ttataaagtc
     1441 atataacttt acaataccta ttatctatga aattgcttta ttttgcaata ctatttttca
     1501 aaaaaatttt tttataacaa ttttatataa taaaatg
//
LOCUS       DASJTC010000202         1534 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.208, whole genome shotgun sequence.
ACCESSION   DASJTC010000202 DASJTC010000000
VERSION     DASJTC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.208"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            281..973
                     /locus_tag="U4B19_002830"
     CDS             281..973
                     /locus_tag="U4B19_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEN2078483.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1300..>1534
                     /locus_tag="U4B19_002831"
     CDS             1300..>1534
                     /locus_tag="U4B19_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2078484.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVH"
BASE COUNT          638 a          198 c          272 g          426 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacatat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgattaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagctggaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 aatactaagt agttcagttt tggagtacaa aatgacaaaa tgtacttcaa aatggactac
     1081 ttttttcatt ttaacttaca taccaaatta catacaaaat gatttataaa atgcaattga
     1141 aaatgataat tgaatgaaaa tttgtgattt tgtggtatta tgataatata acttatagaa
     1201 aaaattgctt attcaactaa cgggcaggat aatgagaaaa ggttttttga tagaaataag
     1261 aaattagatg gtaaattagt taaaaaggct gtgttataaa tgaaagtttc tttgattgct
     1321 gcgatggata agaatagagt gattggcaaa gagaatgaca ttccttggag gattcccaag
     1381 gactgggaat atgttaaaaa tactacaaag ggacatccga taatattagg taggaagaac
     1441 cttgaatcaa tcggaagagc cttacctgac agaagaaata ttattctgac gagagataag
     1501 gggtttacct ttaatggttg tgaaattgtt catt
//
LOCUS       DASJTC010000203         1408 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.209, whole genome shotgun sequence.
ACCESSION   DASJTC010000203 DASJTC010000000
VERSION     DASJTC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.209"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..219
                     /locus_tag="U4B19_002832"
     CDS             <1..219
                     /locus_tag="U4B19_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN2078485.1"
                     /translation="FEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDIDSEED
                     FKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            224..1171
                     /locus_tag="U4B19_002833"
     CDS             224..1171
                     /locus_tag="U4B19_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEN2078486.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          463 a          195 c          293 g          457 t
ORIGIN      
        1 tttgaaatga atgcctcaat gtattctttt aaacctgaat ttctaaaaaa aagtaattat
       61 atttttgatg ggcaatgtgg gataataaaa atgagagatt atctaatttt agatattgat
      121 agtgaagaag attttaaatg gttagaattt ttgtttccta aatttttaga agattcttcg
      181 gagcttcaag atatctataa ttatttatat cggaggtaaa aggatgagct gttatcttat
      241 caccggtggg gctggcttca ttggttctac cttagccaat catttaggag aagaacatgt
      301 ggtaatcgtc gtcgatgact tatcaatggg aaaagtagaa aatcttgata tgacgaaaaa
      361 tatcactttt attgagggag atgtcgctga gaaatccttg atggaagaga tcatgaaagc
      421 taataaattt gattatatct ttcatttagc agcagttgct agtgtggcag attcagttgc
      481 ccgaccagta gaaacacatc gtgtgaattt tgaaagtgta ttaatgattt tagaattgat
      541 ccgcaagtat caacctgatt taaaacggat cgtgtttagt tcttcagcgg ctgtttatgg
      601 ggatgaaccg actttaccaa aaaaagaaga gtccgtgatt cgcccattga cgccttatgc
      661 ggttgataaa ttcgctgcag aacaatatgt gctagattat tgtcatttat atgatgtacc
      721 cggtagcgca gttcgttttt tcaatgtcta tggtccaaat caaaatccga attctccata
      781 cagtggtgtg atttctattc tcgtagatcg ctataagaaa caaatcgtag gagaagcaac
      841 ttcatttaca ttatttggtg atggaagcca gtctagagac tttgtttata tagatgatgt
      901 aatccaagca ttacttttag ttgcgaatga ggaagctgca ctggggaaac aattcaatgt
      961 tggaacgggt aaagcaacca cacttttgga attgattcat gcaatcaatg aaattctcgg
     1021 tgtagaattg acacttgaat acaaagaaga gcgttcgggt gatatccatg actctctagc
     1081 agatattact aagatccagt caattggcta tcaaccgaaa tatggtattt taagtggaat
     1141 gactaattat ttgaaaactg aaatcaatta aaacatacta agataagatt agagggtaaa
     1201 ttcaggctct ttgtcaataa ggactgatga gttttgtaaa atcaaatctg ggatagaagg
     1261 gctctttgtc aaatgatgtt ggtaaagaga aatcttagaa taatttggac agaacagaaa
     1321 gaagaaaacc tctttctgtt ctgtttattt ggttaaaaat gttttgccat gacgaatcat
     1381 aattcgtagt ttgaaattgt aaaagttg
//
LOCUS       DASJTC010000204         1407 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.210, whole genome shotgun sequence.
ACCESSION   DASJTC010000204 DASJTC010000000
VERSION     DASJTC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.210"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            326..1366
                     /locus_tag="U4B19_002834"
     CDS             326..1366
                     /locus_tag="U4B19_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEN2078487.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          549 a          190 c          228 g          440 t
ORIGIN      
        1 tagtaatggt agtatgtttt atatgcataa ggtagttctt aatttatttt ttgttttgac
       61 actggtagta cttaattatc tttaatttta aaactaaatt ttattcatac gtttttctgg
      121 tagtatttaa ttataaataa tatttattta aagtagttct taatgacttt aaaggtagta
      181 cataattcat tttatttctg attttaggta gtatttaatt aaaaaacagt tttaatgcgt
      241 ttgcattttt tatttatctt tgctattttc gaaaagagta ggtaatttta cataaataaa
      301 acaacgtgaa agtagaggat ctacaatgac tgattttaaa ttttttaagg cggatagagt
      361 ttacaacgaa ttattttatc aatttccaaa agtctttatt gtttctgacg aatacaaaaa
      421 aatgaaagat tcaactaaga ttgcctatat gcttttaaaa gcaagattag agatcgcaat
      481 cagcaaacgg caaatcgatg aagaaggtaa tgtttatttt acttatacga caaatgaact
      541 atgtagagta ttaaactgcc aaaaacaaaa agcgatagca atcaaaaaag agttggaatc
      601 ctttggttta ttattacaaa agcagatggg atttaacaaa cagttaggga aaaataatcc
      661 taatagacta tatctagcag aattaaaagt ctcagaaaat gatatctact tactcgaaaa
      721 atttgataga gagaataggg aaaacgttga taaatcagag ggtatgaaaa tcatacccac
      781 cctcgacgaa aaatcagacg ctgaatccct tggggctcaa gagggtatga aaatcatacc
      841 gtgccaaaac gttgataaat cagagggtat gaaaatcata ccagaactta ataataatat
      901 attagacact aatagacaca atatagacac tgaaaaagac cgcctacaag atcaattgtt
      961 gttagacaat tttgagacaa ttatgacaaa cgacagcatt gctacgtttg tccctgaacg
     1021 agtattaaat ttgataaaaa cattttcttc aagttacagt gaagctcaaa aaaccgtcca
     1081 gactattcat aatgcaaaga aaaaagctga aatagaaagt gatatttcga tagtttttga
     1141 agaactcgat agttattatg tcaatgcaga acaagaatta tacacgacac tgttaaaagc
     1201 ctatcaaaaa ttaaaaaccg aaaaagtcga aaatatccag aacctgattt ttgtctatgt
     1261 aaaaaattgg tttatcgaaa aaccaatagc tgctaaagta tcaagtgaaa aacgtttgaa
     1321 ttatgcaagc tccccaagca ctattacgaa agactggtta gagtgaaact ttactgacaa
     1381 agtgattgtt gcttcatgga gtaacat
//
LOCUS       DASJTC010000205         1385 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.211, whole genome shotgun sequence.
ACCESSION   DASJTC010000205 DASJTC010000000
VERSION     DASJTC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.211"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..410
                     /gene="spx"
                     /locus_tag="U4B19_002835"
     CDS             <1..410
                     /gene="spx"
                     /locus_tag="U4B19_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEN2078488.1"
                     /translation="IKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKKI
                     LAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRILI
                     GFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            424..780
                     /locus_tag="U4B19_002836"
     CDS             424..780
                     /locus_tag="U4B19_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEN2078489.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          506 a          207 c          258 g          414 t
ORIGIN      
        1 tgataaaagt atatacttcg cctagctgta cttcctgtag aaaagcaaga gcatggttgc
       61 aagcgaatca acttgaattt gaagagaaga atatttttgc cgaaccgtta acagaaagtg
      121 agatcaaaaa gatcttggca gcgactgaag gaggagttca agatattatt tccactcggt
      181 cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt aactttaaag caaatgatcg
      241 tcttattcaa agaatatcct tcattattga gacgacctat tttgattgat gaaaaaagaa
      301 tactaatcgg atttaatgaa gatgagatcc ggtcatttgt ccctcgtgaa attcgatgtg
      361 ttgccagaaa tctgatctat ttacaaaaca ataacatctt aaattcatag ggagggagcc
      421 ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa caagtctgaa ggcgaaacaa
      481 tggttagaac accatcaatt gccttatcaa tttgttgact tagaagaacg cgatttaagt
      541 acagaagaag cagaagaatt atgcagtttt gaagaagtgc aagcagaaca gctatttgcg
      601 acttggtctg aggcttttca caacattgcg attgaccttt cctgtgtagg aaaaaaacaa
      661 ctgatttttt tatgcacaca acataccaat ctcctcagac gtcctttgat tattatcgat
      721 gaagttctat ttattggata caatgagcag ttgatggaac agcttatctt aaataaatag
      781 actttaactg ttttaatcaa gtcactgagc atttcataca gttgtaacag tcgaacactt
      841 agcatcaagc gtgttatgtg ttcttttttt gactttattt ttctactata gagacatgct
      901 caatttatgt ggtcaaaatt caaatctagg tggggatata gagttaaaaa tatattgaca
      961 tgtgtaaatt tacatgatat attcatttct gttgttttaa gtagcaaagc aataaaatag
     1021 tattttaaaa aacatataaa atagttgttg acatgttaga aatgacatga taagataact
     1081 gagttgttaa aaaagaacca cttgtgaagc ttgattctat cgaaaaaaac ttcaaaaaag
     1141 ttcttgacaa ataacaaatg ataagttatg ataaatgagt tgctgtaaga tttcgaaaaa
     1201 cgaaataaaa aaaactttta aaaaagagtt gacaattact tgtctctctg atatgatata
     1261 aaagttgcta cggcagccaa aaacgaatcg gggaacactc ctgatgaagt agacctttga
     1321 aaactgaaca aagtaagaca aaccaaatgt gtagggcgtc ttgattcaat tcaagacaac
     1381 aaaca
//
LOCUS       DASJTC010000206         1373 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.212, whole genome shotgun sequence.
ACCESSION   DASJTC010000206 DASJTC010000000
VERSION     DASJTC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.212"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            47..>1373
                     /locus_tag="U4B19_002837"
     CDS             47..>1373
                     /locus_tag="U4B19_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2078490.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITS"
BASE COUNT          486 a          192 c          256 g          439 t
ORIGIN      
        1 ctgcatatca agtaattggt atgaggcatt ttttgaggtg taggatatga agttggatta
       61 ccttatagaa gaggaagata gagaaaaact gctgctagct agatatttag aatttgagaa
      121 aaagaattat gtagtgctgc ctaagttata tgaaacatta ggtttgtcta aatttaaaac
      181 aagaaattat atcatagaat taaataaaga atttactgaa ttcaaaacaa agccagagat
      241 ccaaatttca gaagaagcag agatccatgt cgaacgtctg aatttgacgg atatcaaaaa
      301 tttaagaaga atctactttc aacgatcaaa aattgctcga ctgttaacgg agatcgtgga
      361 ttcaaaagta acgatcgaaa aatttgcaga gaatcatttt ttaagcatat caagagccta
      421 tgtaaaaaga agagaattag tctcttttct tgaacagcat caaataaagt taaagaaaaa
      481 catattagtt ggggaagaaa taaacatacg gaacgtatta tttggggtat atttcggggt
      541 ctttaatggt ttttctatgc catttagaga aagcgtcatg aatgaagcaa agagtatcgt
      601 gcaatatttt gaatatctgt tcaatttaaa actgtctgca acaaaacgaa tcaaattcaa
      661 tttgctaacc actattagtt tgttgagggc ctatgggaat caaccaatcg gaaaacgttt
      721 cttttcagat gaatacttta agagctcaga aggaatgaag atcatcaagg atttatcaca
      781 tatttctttt cttcatatcc ctaaagagca gatcattgat gagatttctt atatcatgtt
      841 gtttttgatc acggaagaag aatttgaatt agatggcgca tttggacttg cggaagctca
      901 ttttcatgat atccacatga ttagtcaaaa aatgtcagtg gaattgattg atcaattacc
      961 attttcgatt gaaatctctg aagaagaaag actatatata cgaaaagaac ttcgtatggg
     1021 tatgatgcat gtcaatcgaa aaaattggat attcgacttt gagatgacta gctttacgtc
     1081 taaaaagcag ctacagtttt tcttcgagac gtatccaata ttcagtacag caatccaaaa
     1141 ggtcatacaa gagaataaac gctatttctt gggaaaagat gaacgtttca tgattcgctt
     1201 attttatgat tatctctttt tattggtcag tatttgtccg gtatcatatt ttgaagcacc
     1261 gattcatgtc tgtattgatt tttcgcaagg atctgcttat accgattaca ttatttctca
     1321 agtgaaagga tttaaaaatt acaatattgt gattgagagt cgaatcactt ctc
//
LOCUS       DASJTC010000207         1367 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.213, whole genome shotgun sequence.
ACCESSION   DASJTC010000207 DASJTC010000000
VERSION     DASJTC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1367
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.213"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..1145
                     /locus_tag="U4B19_002838"
     CDS             6..1145
                     /locus_tag="U4B19_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HEN2078491.1"
                     /translation="MLINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFNS
                     GNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKGI
                     TMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPLK
                     RQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVTD
                     GLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLVYQASSKEEAN
                     NQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEGFNKQLKKYTK
                     RKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          495 a          193 c          227 g          452 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttcaaatta gtttatcaag caagttcaaa agaagaagca aataatcaaa
      841 taaggtttat gatagataaa tggaaaaagc agtatccacg agtggttaaa ttgctcatga
      901 atcctgctat attaaccttt tataatttcc ctccgtcaat cagaagaact atttattcaa
      961 ctaatttgat tgaaggtttt aacaaacaat taaagaaata taccaaaaga aaagagcaat
     1021 ttcctaatga agaatctctg gagagatttc tagtatctca attcaacaac tataatcaaa
     1081 agtttctatg ccgagttcat aaaggtttta aagaaataca tgatacatta gaatcaatgt
     1141 tttaacttga tcaacagaat gggcttttcc atttacacaa aattattgac gctcccgaca
     1201 aaattatgat cttgcgtaaa gttataaaat attaataaaa atattttatt aatgaaaagc
     1261 ttggtagcac ggttattcat atgctaattt atttcacttt atagtaaggg gagttcttag
     1321 tatattgttc aaacttaaat ttcatataat ttaattatgt aaattag
//
LOCUS       DASJTC010000208         1364 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.214, whole genome shotgun sequence.
ACCESSION   DASJTC010000208 DASJTC010000000
VERSION     DASJTC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.214"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            82..>1364
                     /locus_tag="U4B19_002839"
     CDS             82..>1364
                     /locus_tag="U4B19_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEN2078492.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DASJTC010000209         1363 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.215, whole genome shotgun sequence.
ACCESSION   DASJTC010000209 DASJTC010000000
VERSION     DASJTC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.215"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..732
                     /locus_tag="U4B19_002840"
     CDS             <1..732
                     /locus_tag="U4B19_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEN2078493.1"
                     /translation="AITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDS
                     FWNIVTGIVNSAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVK
                     EKWNALKDTIKGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLD
                     QTMTNLVKTPSVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKIT
                     TKIKTELTRQMKRDAGAVGGTLYAT"
     gene            722..>1363
                     /locus_tag="U4B19_002841"
     CDS             722..>1363
                     /locus_tag="U4B19_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEN2078494.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLF"
BASE COUNT          519 a          165 c          250 g          429 t
ORIGIN      
        1 gccattacta cttggaataa tattaaacag tttttcaaag atacttggaa aaatattaaa
       61 gatacggcat acaatacgtg gatttctata aaaaatacca tgattaatac atggaataac
      121 attaaggact ctttctggaa tattgttact ggaattgtta attctgctga aaatgcatgg
      181 acaaatctaa aaaatggtgt ttcaaatgct attaatcggg taaaagaaat ctttaattcg
      241 ttaagggaag tcaatttatt tgaaattggc aagaacatca ttgatggact tataaatggt
      301 gtaaaagaaa agtggaatgc tttgaaagat actatcaaag gtattgcagg taatatcaaa
      361 aattggatta aaggagcttt agatattcat tctccatcaa gatggatgcg tgatatggtt
      421 ggtaaaaata ttgttcaagg tattatcgtt ggtattgata aagagcaaag taagttagat
      481 caaaccatga caaatttagt aaaaacaccg tctgttcaac ctgtagtaaa tggaataagt
      541 cacctaccaa ttacacaaac aaagcaaaat atatctgcta gtgaaacaaa agaaattcat
      601 ttacatttaa atgtttatgg agatttacct gattcaatgg ttcgaaagat tactacaaaa
      661 attaaaacag aattgacaag acaaatgaaa cgagatgctg gtgcagtagg aggaacatta
      721 tatgcaactt aagagaggac aattttttat taatcaacat tattcttctg aatttaatgt
      781 gtatattcaa aacaggcctg cttctgtttc agctagtcga gtaattgaat tgagagagcg
      841 agaaggaaat gattctatca ttattgataa aggctattac aaaaatgtga ctagaaagat
      901 tgagtgttac tataaagcac cttcaattga tatggtgcag gagtgggaag atcgaatcac
      961 tgaatggtta gatatgtcgt cttatagtga ttttattctt tactatgatg aacaatatat
     1021 ttatcaagct gtagtaatag aaggacctga atttaaagga acaagaaaaa caggaaatat
     1081 tgtccctttt gaatttacag tgagtattcg accatttaag gaaaattaca atggtagatt
     1141 tactattcga caaacagaaa cttttgaaat ttataaccca gagaagtatt cttcaaaacc
     1201 gcttattaaa ttgagtggtt ctggagatgc ttctttttat attaataagg ataaatatga
     1261 tttgaaatcg ttggacagag aattatttat agattctaaa ttagaagagg cttatcgaaa
     1321 attagatggt aatttagaac atcaggatca ggtcacctta ttt
//
LOCUS       DASJTC010000210         1355 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.216, whole genome shotgun sequence.
ACCESSION   DASJTC010000210 DASJTC010000000
VERSION     DASJTC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.216"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1355
                     /locus_tag="U4B19_002842"
     rRNA            <1..1355
                     /locus_tag="U4B19_002842"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DASJTC010000211         1320 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.217, whole genome shotgun sequence.
ACCESSION   DASJTC010000211 DASJTC010000000
VERSION     DASJTC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.217"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            145..1020
                     /locus_tag="U4B19_002843"
     CDS             145..1020
                     /locus_tag="U4B19_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078495.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1165..>1320)
                     /locus_tag="U4B19_002844"
     CDS             complement(1165..>1320)
                     /locus_tag="U4B19_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HEN2078496.1"
                     /translation="EWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKIYPAT
                     KEICHDL"
BASE COUNT          468 a          245 c          228 g          379 t
ORIGIN      
        1 tcacattctc taagaaaaga aaaggagaat gaatttttga caattcgagc aaaagtgatt
       61 atcaaatatg taaaatcagc tacttttttc ttaaacgttt ctttatacta aatataaata
      121 agtgtttcat gaaaggagaa ctagatgaat aattggcttg agtattttcc tgaaaacgtt
      181 ttggaacgcg gttacagcta tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc
      241 aagtacttgt ccgcaacagt ttctggcacg gaagattata aggtagtgat cacttgggat
      301 gaaaagacca acatgacttg tgattgtcta tatgccatag aaggaaaaaa atgcaaacac
      361 atggctgctg tcttattcgc ttacgaagaa cgtccaataa aaaaatccaa ctactcactt
      421 tccgaactct cgtcccttgt ttcctccgct agttcctctc ttgtacggga gcttttaaca
      481 gagattctta tagaacatcc gcaatttatc gaacgcttca aagttaagat gccatttcat
      541 gcaataaatt attcagataa attaacaact atcatccata aatacgatca tattatcaag
      601 aaaaacaaaa acaggaaaac agcgaagttc atcatggaga tgcgaaagtt catccaagaa
      661 gcagttgaat cgctcattca gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa
      721 gtgattgcta ctctcgaaac attctattgg gagcctgagg atgaacgaac ccttcttctg
      781 atagaggact gttattattt atggaaagaa ttattagcag aagcacctca tgcagaaaaa
      841 aggcaaatgt tctcttggtt cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa
      901 cgctactcca taaaaatcct gaaagaagac tttcgggaaa aagaattttc taaccaaaag
      961 aaaaaaatag ataaaaaaac taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag
     1021 caagtaaacg ataacaacca tcctaaatcc acaagaaata cagcagacct tgctttaaaa
     1081 acacgaatca tgcaaaggat gaagtaaaga agacttaacg cgtacccaat attagtcatt
     1141 tgactattag gtacgcgttt tgttttataa gtcatgacaa atttccttcg ttgctgggta
     1201 gattttccgg cattgttgat agattttatg atacttttct acattttgta agagaggttc
     1261 cactgcatct gtatattgga taaaattctg acaacaactt tcaggtgtgt cgaaccactc
//
LOCUS       DASJTC010000212         1304 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.218, whole genome shotgun sequence.
ACCESSION   DASJTC010000212 DASJTC010000000
VERSION     DASJTC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.218"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            38..658
                     /locus_tag="U4B19_002845"
     CDS             38..658
                     /locus_tag="U4B19_002845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078497.1"
                     /translation="MTIYPCSFNVDDQNDYGEKIVNKSLESTLNLAYLGTINNIVDLE
                     AINELLKTCKRKFGNKINLYFIGKGESKTKLADICEKNHIEFVDCGVIYDNAEKKVIL
                     DKCHFGLNLFKSNTIIGLSLKSIEYFRFGLPIISNLSGDTKEIVKRYNVGINYNSTVD
                     ENEMLKFYQCNKNINENVRKIYATIFSEQVLKMKWNEVYKVMNDEK"
     gene            648..>1304
                     /locus_tag="U4B19_002846"
     CDS             648..>1304
                     /locus_tag="U4B19_002846"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEN2078498.1"
                     /translation="MKNKIGIIIVTYNPDNNIKLLVDTLIQEKQNKLIIVDNGSDNIN
                     SFQFEMMENLILKKMNENIGLATAQNIGIEIAKKEKCDYILLLDQDTIIQKNLSLKLL
                     EEFTNIEAKDKDIGIIVPNYFENSNDESVSFTCKNERGRYVKKHFASTSGSMKIAFAN
                     SSGMFFKSELIDDVGNMKDEFFIDQIDVEFCKRVANNGYNIYVTNQVYFYHHVGDGEN
                     L"
BASE COUNT          526 a          143 c          224 g          411 t
ORIGIN      
        1 caaaaaattc ataaaaattt ccggagaatc aaaaaatatg accatttatc catgttcttt
       61 caatgtagat gaccaaaatg attatggaga gaaaatagtc aataaaagct tagagtctac
      121 gttgaacctt gcttatttag gtacgattaa taatatcgta gatttagaag cgattaatga
      181 attattgaaa acatgcaaaa gaaaatttgg aaacaaaata aatctttact ttattggaaa
      241 aggtgaaagc aaaactaaat tagctgatat ttgtgaaaaa aatcatattg aatttgttga
      301 ctgcggtgtt atatatgaca atgcagaaaa aaaagttata ctcgataaat gtcattttgg
      361 actaaattta tttaagtcaa ataccataat tggtttaagt ctgaaatcga ttgagtattt
      421 taggtttgga ttacctataa tttcaaattt atcaggagat acgaaagaaa tagttaaaag
      481 atataatgtc ggaatcaatt ataatagtac cgttgatgaa aatgagatgt tgaaatttta
      541 tcagtgtaat aaaaacataa atgaaaatgt tagaaaaatt tacgccacta tattctctga
      601 gcaagtattg aaaatgaaat ggaatgaagt gtacaaggtg atgaacgatg aaaaataaaa
      661 ttgggataat tattgttacc tacaatccag ataataacat aaagttatta gtggatacgt
      721 taatacaaga gaaacagaac aaacttatta tcgtggataa cggctcagat aatattaact
      781 cttttcaatt tgaaatgatg gaaaacttaa ttttaaaaaa aatgaatgag aacattgggc
      841 ttgcgacagc tcaaaacata ggtattgaaa ttgccaaaaa agagaaatgt gattatatcc
      901 ttttactaga tcaagatacc atcatacaaa agaatctttc tttaaaatta ttagaagagt
      961 ttaccaatat tgaagcgaaa gataaggaca ttgggattat tgttcccaat tactttgaga
     1021 atagcaatga tgaatctgtc tcttttacct gcaaaaatga gagagggaga tacgtaaaaa
     1081 aacactttgc ttcaactagt ggttccatga aaatagcatt tgcaaattcc tctggaatgt
     1141 ttttcaaaag tgaattaatt gacgatgttg gaaatatgaa ggatgaattt tttattgatc
     1201 agattgatgt tgaattttgt aaaagggttg caaataatgg ctataacatt tatgtaacaa
     1261 atcaagtcta tttttatcat cacgttggag atggagaaaa ttta
//
LOCUS       DASJTC010000213         1282 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.219, whole genome shotgun sequence.
ACCESSION   DASJTC010000213 DASJTC010000000
VERSION     DASJTC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.219"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(62..280)
                     /locus_tag="U4B19_002847"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="U4B19_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="U4B19_002848"
     CDS             complement(546..740)
                     /locus_tag="U4B19_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078499.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="U4B19_002849"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="U4B19_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          453 a          215 c          175 g          439 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt ac
//
LOCUS       DASJTC010000214         1280 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.220, whole genome shotgun sequence.
ACCESSION   DASJTC010000214 DASJTC010000000
VERSION     DASJTC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.220"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..875
                     /locus_tag="U4B19_002850"
     CDS             <1..875
                     /locus_tag="U4B19_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEN2078500.1"
                     /translation="LNKREKNNLKKKMPIPFDSEVKGLLNKLTISNIDSTREKIIKIF
                     DKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIEEEKVRR
                     EIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKELEVIRE
                     NVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHA
                     MIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVTFTKLA
                     KAEQYRRSLELSKD"
     gene            947..>1280
                     /locus_tag="U4B19_002851"
     CDS             947..>1280
                     /locus_tag="U4B19_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078501.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSN"
BASE COUNT          567 a          148 c          207 g          358 t
ORIGIN      
        1 ttttaaataa acgagagaaa aataatttaa agaaaaaaat gcccattcca tttgattcag
       61 aagtgaaagg attactcaat aaactaacta tttctaatat agactcgact agggaaaaaa
      121 tcataaaaat atttgataaa attaataatt tattcaagaa tgacaatgct gaattaagga
      181 agggtctttt agagtttaaa cttcaagaac tagaacttca ttactcttat ttagtgaaaa
      241 ttaccgaaga aaaagaacaa caaaaagcaa taaaagaaca gatgatagaa gaggaaaaag
      301 ttcgtagaga aattgatcgt gaaaagaaac gtattgataa agaacaacgt caattcaatt
      361 ctgaaatttc caaattaatc gcatatatgc aaaaagctaa tgcagatgtg gaaaaagaat
      421 tatatgcaaa taaaattcaa gaactggaag aaaaattaaa agaattagaa gttatacgtg
      481 aaaatgtatt acaaagagaa ttaaatacta gagccggtta tgtgtatgta attagtaata
      541 ttgggtcttt tggtgaagac atatataaaa ttggtatgac tagaagatta gaacctatgg
      601 atagaataaa agaattgagt agtgcatcgg ttccatttga atttgacgtt catgcgatga
      661 ttttttcaga ggacgctccg tctttggaaa ctaaattgca taatcatttt agaaaacaag
      721 aagttaataa aattaatcaa agaaaagaat ttttcaaagt ttcactagac gaaatagaga
      781 aagttgtttt agaaaattat aatggaacag taacatttac aaaattagct aaagctgaac
      841 aatatagacg atctttagaa ttgtcaaaag attaaatata taaaaagcct tcgggctttt
      901 tcttttaaca attaatagga acgtatattc gaaaggaata gaaataatgt ggttagaaaa
      961 aataaatgac aaaaaattta agtacaccga acgttatacg gatcccttaa ctgaaaaaaa
     1021 gagaaaagtg tccgttactt tatctagcaa ttcacgccaa gcttggaatc aagctaactt
     1081 acttttgaat gaaaaaatcg cagaaaaaat aaaacgaaat gaagaattac cactaacttt
     1141 tggagaatta aaaactaaat gggatgaaaa atacaagcca actgtaaaag aaagttccta
     1201 tcgaacaact caggtttatc tatcattaat ttcaaaatat ataaaagatg atgtacttgt
     1261 aaaaaacgtt aatagtaatc
//
LOCUS       DASJTC010000215         1247 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.221, whole genome shotgun sequence.
ACCESSION   DASJTC010000215 DASJTC010000000
VERSION     DASJTC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.221"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            56..>1247
                     /locus_tag="U4B19_002852"
     CDS             56..>1247
                     /locus_tag="U4B19_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2078502.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLN"
BASE COUNT          383 a          248 c          245 g          371 t
ORIGIN      
        1 acaccctgaa atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc
       61 tcatactcag cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga
      121 aaattgttta actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct
      181 tctgtacaag ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg
      241 ttgggggacg acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct
      301 ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac
      361 ctctgttgct gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg
      421 cttaaaagag aatacttcca tgacggaaat cgctcgtcag aaaaatattt ccacttcttc
      481 ggtctatcgt gtcatgaagc gattctaccg cccattgaat cctttcaaac agactttgcc
      541 aaaggtctta tgctttgatg aattcaaatc ggttcgaagt gttgccagtg caatgagttt
      601 tatcatgatg gacggacaaa gccatgcatt attggatatt gtcgaaaatc gacgcttgcc
      661 ttatttagaa cgctattttt ctcggttttc attagcgact cgtgaagccg ttcaattgat
      721 tgttattgat atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc cgaatgctca
      781 gttgatcatc gatcgtttcc acatcgtcca acatattggc agaactttct tgaatcatcg
      841 agtgaaagaa accacggtac gacttaaagg aaattcccac tctcaacgag ggttagggaa
      901 aaaattgaag cgctattgga aactcctaca aaaagaagaa gcaaagttaa actatgaaaa
      961 acgggtttgg cgtgccagtt ttaaagatta tttgactgaa agtgagattg ccgatcgact
     1021 gttagctgct tgtcccaact tacgccaagg ctatcagtta taccaagaca ttctttatgc
     1081 ggtaaagaaa agagatcagt cactatttga ggattgttta actagagaca ttagcggtct
     1141 gccagaaacc tatacaacca ctttacgaac gtttaaaaag tttctgccac agatccaaaa
     1201 tgcgcttcac tacagctatt caaacggacc attggaatgc ttaaaca
//
LOCUS       DASJTC010000216         1238 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.222, whole genome shotgun sequence.
ACCESSION   DASJTC010000216 DASJTC010000000
VERSION     DASJTC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.222"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..409)
                     /locus_tag="U4B19_002853"
     CDS             complement(<1..409)
                     /locus_tag="U4B19_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEN2078503.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRD"
     gene            complement(568..834)
                     /locus_tag="U4B19_002854"
     CDS             complement(568..834)
                     /locus_tag="U4B19_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2078504.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(824..1180)
                     /locus_tag="U4B19_002855"
     CDS             complement(824..1180)
                     /locus_tag="U4B19_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEN2078505.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          338 a          247 c          154 g          499 t
ORIGIN      
        1 agtccctgta cttcttaact tctatatact cttcagccaa aacttgtgga actggttcat
       61 ctaagaaaaa aacccctacc tcgcctttgc tatttttata aaagtaatca atttctgcta
      121 tagttcccga tctataatta tcaactggag aacccaactt atggccaaaa atcgctaata
      181 atatatcact attttctaca atttgtttat taataacact ttgcccagag ctattattta
      241 tataagtagg aatcgcattc gtttcccatc gaattggcat caaaattact tttgtattat
      301 ctgtattttc tatattccat ctatatatgg tttcttctac ttcattcctc tttgctgcta
      361 tatcacctgg tgatgctata aatactttta ttagatttat ggttttcatt tattgtacct
      421 ttctctttat gtctctttaa agtaattttt gtataaaatt tattgacttt tacactatag
      481 gacaactttt atctatttag tacaaaaagg cccactaaac taattgatta gttaggagcc
      541 tattacaggt atattttcaa ctcgctttta agcaaattct actaccagct ttttgcctaa
      601 tgccctagcg atatcatcaa gtttatcgaa agatacgtta tcccctctct caattcgagc
      661 aatagtagat tgagttgtat gtgctttttg tgcaagctcc gcttgagtta ggcctgcttc
      721 ctcccgtgca tgataaagag caaccgcagt ttcgagtttt cttccttcac ggtcatatgc
      781 ttctgcaaaa tctttgtctt tttgcttttc ttcaatcaat tttttaatca tcatttgtga
      841 accactcctc tctaagttct tttgcatgtt taagttcact tattggtgtt ttctccgttt
      901 ttttagtaaa tccatgggta atgacatacc ttccattgac cacatggaaa tagatagctc
      961 tttggatgtt tgatcctacc ttacttctta attcgtacaa attagtgtct aattttttca
     1021 cccatttcaa ccgttgagct actagcatcc cattcttttc tgtttcttct attgtacgta
     1081 aaagcttcgc actatctttt tttggtaatg tttccaacca ttctaaaaat tcatcatgac
     1141 cattaggtct tttaaatgtt tcaaaagata ttttttccat acccaattat atcatatatt
     1201 gattttatat tatagtatat aacataaaaa ttcgaatt
//
LOCUS       DASJTC010000217         1226 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.223, whole genome shotgun sequence.
ACCESSION   DASJTC010000217 DASJTC010000000
VERSION     DASJTC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.223"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            111..983
                     /locus_tag="U4B19_002856"
     CDS             111..983
                     /locus_tag="U4B19_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEN2078506.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          368 a          224 c          286 g          348 t
ORIGIN      
        1 aaaaaatagt agttatttac ttgtcatgtt actaaataaa aaaataattt aaatatgata
       61 tatagtcatt tatttagtga tttcattcta aaataaaggg agaaatgtct atgctatacg
      121 gtgcaattga agcaggcggc acaaaatttg tgtgtgccat tggcgatgaa gaaatgacga
      181 tcaaggaaag agtgagcttt ccgacaacga cacctgagga aacaatgcct ttagtcattg
      241 acttcttcaa acaataccaa gcagatttag cagggattgg gattggctca tttgggccta
      301 tcgatatcca tcgcgattcc gccacttatg ggtacattac ctcgaccccg aaattggcat
      361 ggcaaaattt tgatttcatc ggtaccatga aaaaagaatt tccgatcccg atttcttgga
      421 caacagatgt caatgcggct gcctatggtg agtatgtctt cggcagtggc aaaggattga
      481 gcagtgtcgt ttactatacg atcggtacgg gtgttggcgg cggtgcctta caagaagggc
      541 gctttatcga agggtttagt catccagaaa tggggcatat gctcgtcgtc cctcatccaa
      601 aagatgactt tgctggcagc tgtccatttc atggcaactg tttagaaggg atggccgctg
      661 gacccgccat cgaaaaccgc ttagggaaaa aaggacaaga ggttgcagaa gatgatcctt
      721 attgggaaat cgaagcctcc tatattgctc aatgtgccta caatacaacc ttgatgtttt
      781 ctccagatgt gatcattttt ggcggtggtg tgatgaaaca acgccatatg ctgcaaaacg
      841 tccacgacgc atttgcaaaa ttggtcaatg ggtatgtcga aacacctgag ttaagcaaat
      901 atatcgtgac cccagcatta gaagacaacg ctggaacgct cggttgtttg gcattggctc
      961 gagaagcggt cttacattca taaacgaaaa aaggctcttc gacatttagt gttggtagag
     1021 aaagatacaa ctgaaaattt agtcataacc agttaggcag agtgcttaac tggtttttgt
     1081 tttacttgga agatcaagcc ttgaatgaga taaattcttg ctcgaaagtt atggaaattt
     1141 cgatagccgt aggccacttg tattgtgcaa tagtttttta caaaaaaaag ggggtgcgga
     1201 tatttttttg gaccttttta acaaat
//
LOCUS       DASJTC010000218         1223 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.224, whole genome shotgun sequence.
ACCESSION   DASJTC010000218 DASJTC010000000
VERSION     DASJTC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.224"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            392..553
                     /locus_tag="U4B19_002857"
     CDS             392..553
                     /locus_tag="U4B19_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2078507.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            572..1066
                     /locus_tag="U4B19_002858"
     CDS             572..1066
                     /locus_tag="U4B19_002858"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2078508.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            1059..1220
                     /locus_tag="U4B19_002859"
     CDS             1059..1220
                     /locus_tag="U4B19_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078509.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          478 a          183 c          223 g          339 t
ORIGIN      
        1 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
       61 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tcttaaaaga
      121 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
      181 tgaaaacaac cctagaaata tataactagt ccaatgaaaa aaaagcgaat gccgtagaaa
      241 acaaggattc tgcgtacatt catatctcat tttgcgagca tatctgctat tactgtgact
      301 aaatgttaat atggttaaaa tgaccgttat attttttata taaatatcgt ataattagta
      361 tgaatataga agaaggaaag aaggtgcaat cttggaacct aaagacaagt atttagatta
      421 cgctagggtc aataaagata aatttattga aacaatcatc aagggaaaaa tccctgatga
      481 tgaaaaagat gcggtattca tggctggaag cccaggagca ggtaaaacag aggttgcact
      541 aggtttagct taaaattatg acaaccatgt tgtgattgat gcggactatt ttagagcaca
      601 atttccagaa tacaatggaa agaactcaag tgtttttcag aaagcttctt cttggttagt
      661 tgaacaatca ttaaaatatg tactggaaca tggctattct ttcatactag aggatacttt
      721 tgctatcctt agtgcagaga aaaatattat tcgagccctt aaaaataact ttcgcgttac
      781 gattttctat gtttaccaag atcccaaagt tgcctgggat ttcactcgaa aacgtgaact
      841 agctgaagga cgtcatgtgc caaaagaaat gtttatcaat gcgtttttta agtccagaga
      901 gaatattgaa aaagtcaaaa atagacatcc tgaagtagtc cttcacataa tgattaagga
      961 ttatcaaaac gacatttctg aagtgcatta tgcagcagat aatattcaat tagtactacc
     1021 aatacaatat acttcaaaag agttggagga ggaattacat gactgagaaa gaacaagtac
     1081 aacaaatcgt gaagaagtat aataaaagta tcgctgattt atcagaaaac gcctcagcga
     1141 aagaatttaa aacagtaatg aattacgtag cagatgaagc aaatcgtaag cagcgaaaac
     1201 tagttgggtt ggataaataa att
//
LOCUS       DASJTC010000219         1195 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.225, whole genome shotgun sequence.
ACCESSION   DASJTC010000219 DASJTC010000000
VERSION     DASJTC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.225"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..1076
                     /locus_tag="U4B19_002860"
     CDS             <1..1076
                     /locus_tag="U4B19_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN2078510.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DASJTC010000220         1150 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.226, whole genome shotgun sequence.
ACCESSION   DASJTC010000220 DASJTC010000000
VERSION     DASJTC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.226"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..385
                     /locus_tag="U4B19_002861"
     CDS             <1..385
                     /locus_tag="U4B19_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEN2078511.1"
                     /translation="LKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKIQKVADYFNV
                     STDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPLDDEDKEKLY
                     DAIQLALALTKVKAKKKFTPKKYRQ"
     gene            398..850
                     /locus_tag="U4B19_002862"
     CDS             398..850
                     /locus_tag="U4B19_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEN2078512.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            941..>1150
                     /locus_tag="U4B19_002863"
     CDS             941..>1150
                     /locus_tag="U4B19_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078513.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
BASE COUNT          477 a          144 c          180 g          349 t
ORIGIN      
        1 attaaaagaa cgttcaatga cgttggcaga acttgaaaga aagttaaatt tcggaaatgg
       61 aagcatgtct agatggaata aaagtactcc atctggagat aaaatacaaa aagttgcaga
      121 ttactttaat gtatcaactg attatctttt aggacgtact gataaacgcc attattatga
      181 tttaacagaa aaagataaac aagatattgc tttacaagct gaaaaattac ttgaaggctt
      241 gaattcagat gctgaaacaa attattatgg tgaaccttta gacgatgaag ataaggaaaa
      301 attatacgat gccattcaac ttgcacttgc cttaaccaaa gttaaagcaa agaaaaaatt
      361 cactcctaaa aaatatcgtc aataggtggg ctttggtatg catttaaaca aatactggat
      421 attagaagaa atagaaaaat tatttgtaga gtttggatct gatgcgatta atccatataa
      481 aataatcaaa tattctgata tagatttaat tgaaactgat ttagggaata aaacattagg
      541 acaaacgata cacaataaaa gatgttatgt gatattaatt aatcaaaacc tatcaccttc
      601 tatgaaaaga tttgtattat ggcatgaaat agcccacgtt agactccata aaggtatttg
      661 cacagcagct tttagagcaa acaaccttag ttgtatgatt tatggtatag aagctgaagc
      721 taatatgttt gcaattgaaa tgttaactag aacaatagat gaacaagatt tatctgatat
      781 gagtcagttc caaattattg aatatttagg gcttcctcat aatcttgaac atttagtatt
      841 acaacattaa aaataaaaaa gcccatgctt tcacatggac tctttcctca tttctgagta
      901 aactctacaa taattatatc aaagaaatga ggagttttga atgttattat tgattggact
      961 tattttatta attataggaa tcactaaact gtcgaaagct aacacaataa aaaaagaagc
     1021 ttatgaatta gaacaaaaaa atgatgaaaa actaaataaa ttacatgata gagaaagatt
     1081 tatattaagt aaggaagaaa cactatcaga tttaaataaa caaattacta tgttaaaaaa
     1141 tgatattaat
//
LOCUS       DASJTC010000221         1088 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.227, whole genome shotgun sequence.
ACCESSION   DASJTC010000221 DASJTC010000000
VERSION     DASJTC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.227"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(41..850)
                     /locus_tag="U4B19_002864"
     CDS             complement(41..850)
                     /locus_tag="U4B19_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEN2078514.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT          329 a          206 c          189 g          364 t
ORIGIN      
        1 aaattgtcac aacctctttt ttgtggaatc taattctcta ttatagttta agtagttttt
       61 ccagataaac agctaaaccg tcttcctgat tgctcaaagg agtgacatca tttgcaatgc
      121 ctttgagttg gtcagtaccg ttagccatag ctactcccca acctgcataa tccaacaatt
      181 ccaaatcatt atgctcgtca ccaaaggcaa tgacgtcttt ttgatcgata gacaggtagt
      241 tagcaatctc ttgcacgcct tttgcttttt ggattccttt tgaaacaatc tctaagatgg
      301 cagtaggtcc accccatgtc cgaacatcga cataactgcc aaattgacgt gttaattcag
      361 cagatacagc tcctgcaaaa cgtttgtctg ttcggacaag caaagatgta ggatttgtcg
      421 tcaaattttt aggagaaagc aaattcttct ctcctggacg agaagaagca aaaatttttt
      481 catcaaagaa atcaaaactg tcaataaaaa aagtatcacg attttcagca gcgataaaat
      541 ctaaatttag ctgctttttt tgcgataaga tttcaaaagc gatttcacga ttgatcaacg
      601 tttctctttc accatcccag tgctgattag ggatgtgtac taatgcgccg ttaaaattaa
      661 ccatcggtgt tgttaattct aattgttggt aaaactgatg gctcattcgg aatgggcgtc
      721 ctgtagcaat actgacatga tgaccggcat tgattgcttt ttttagagtc tcttttgttt
      781 tagatgtaat catcgaatct tgattgaggg tagttccgtc cagatcgatg gcaattaatt
      841 ttttattcaa taagggtcct tccttcctca actagcattt gttttagtct agcatatttt
      901 aggtaattaa caagagcaat cagctattcc taacaaaaac tacagttatg gctatataaa
      961 tagctaaaca taagctaaac acgaacgtta agtattattt tcgctttttt tgttaaaaaa
     1021 acgttgaaaa gcgaatgaaa cgtctatata atttgaaatt gttcgatgga tttgccctat
     1081 tttactta
//
LOCUS       DASJTC010000222         1083 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.228, whole genome shotgun sequence.
ACCESSION   DASJTC010000222 DASJTC010000000
VERSION     DASJTC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.228"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..1024
                     /locus_tag="U4B19_002865"
                     /pseudo
     CDS             1..1024
                     /locus_tag="U4B19_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT          443 a          125 c          195 g          320 t
ORIGIN      
        1 atgaatccaa cattgattac taagaaagaa ttactaaaaa aattagatat ttctactggt
       61 gtattagcta atttaattag aaatggcatg ccaaaagagg gagaaatgtt taatcttgat
      121 aagattatta catggcgaga aaattggtct aaaaacatat taggtgagtt agaagttggt
      181 agagtatata ctaacaaaga aatttcagag aagtttaaat gttccaaaca gggtggaatg
      241 agaagatctc atcaaactaa tactcttgta ctgtttagtg atcaaacagg aagtaatgtt
      301 tataaagata agtggttaaa tggtatttta caatataccg gtatggggct aaaaggggat
      361 caagtattgg ataagaatca aaataaagtt ttagccaatt caaaaagtaa ttttgtaaag
      421 attcatttat ttgaaacatt taaaccaaaa gaacacacat atctgggaga agtatattta
      481 gcaggacaaa tatatactgt taatgaaaaa gatagttcgg gcaacagcag aaaagtatat
      541 aaatttccat tagctttaat aaatcaagag caattgattg aagataaaga tatataacca
      601 ggaagaaaat caaactagac atattcgtaa tttatctaat gctaaattag aggaagaagc
      661 aagaaaagtt agtaattata ataaaaaaat ggctgcaaaa tatgttaacg aagaggagtt
      721 ttgcttctca aaaacaaaaa tatataaacg taatcccttc gtatctgaat atgttaaacg
      781 acttgcaaaa ggtatttgtc aactttgcca agaaaaaggg ccatttataa aagacggtgt
      841 accttattta cattgtcatc atatagaata tttgtcgcaa ggtggaaaag atgtgattga
      901 aaactgtatc gcgttatgtc ccaattgtca tgctagaatt catgaacttg agttacctga
      961 cgataaagaa aaattgctga agatcgttga aaaaagagaa aatagctttt ctaaaatacc
     1021 gtaatttatt tttataggac aaataagcta atgtgagcta tgccttctat ataagactaa
     1081 gtt
//
LOCUS       DASJTC010000223         1077 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.229, whole genome shotgun sequence.
ACCESSION   DASJTC010000223 DASJTC010000000
VERSION     DASJTC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.229"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..569
                     /locus_tag="U4B19_002866"
     CDS             <1..569
                     /locus_tag="U4B19_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEN2078515.1"
                     /translation="INAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERK
                     EEFDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQG
                     VRHESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEF
                     PIEEGALIIATVDDPKQNVFTLEKVGVI"
     gene            569..>1077
                     /locus_tag="U4B19_002867"
     CDS             569..>1077
                     /locus_tag="U4B19_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078516.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          381 a          139 c          240 g          317 t
ORIGIN      
        1 tcatcaatgc tgtttcaaaa acagaaggag aaatgggttc ttattggcaa tcaattgaag
       61 atttaattgc tgatatgaca gattttatta atgaaaataa aggcgatttt acggactgga
      121 tgaatgaaag gaaagaagaa tttgatcgct ggagagagga acaggagaat agtttccaag
      181 attggagaga aggacaagaa tcagattact tgtcatggtt tgaatcaatc aaggatattt
      241 tgaagtctat cgatccaggt ggagtaatgt tagccgaatt aatggatgca cgtgtagatc
      301 ttcaaggtgt acgccatgaa tcgatttctg aacgtttcct atccgatcta aattatttgt
      361 atcaaaaaat gaaagcagca cttttcacga ttgaatacgg tgaaattgaa gtgactgata
      421 ttcttcaaga cgatctcttt tcagataatc acgaagtcga aaaaattgaa acagtagaat
      481 ttccaattga agaaggagct ttgattattg caactgttga tgatccaaaa cagaatgttt
      541 tcactcttga gaaagttggg gtgatttgat ggttaaagta aagagaatga tggaaactga
      601 tgaatctggt gttaaacgtc aatttatgcc aattacacat gtctctgcag ttttaggctt
      661 agaaaaaatg atatcaggac aatcaaaagt tctttcggtt aatggtaaat atggtgcagt
      721 cattttaacg aaagctgatt taggattaga gaatgcaatt actgaactac cttatgcaag
      781 cgagacaagc gatggtattc ttactgctga aatgtttcaa aagatcgtaa atggagaagg
      841 tggaacatat attttgccga ttgctactcc tgaacaatta ggaggtataa aagttgggga
      901 actcttagaa gttactgaag aaggtgtttt atctgcaact aagcaaacag acttcaattt
      961 ctctgaagaa ttaaaaggaa aattagaatc tctaaaaata ttaaaagcag gtgcaaatat
     1021 atcaattgca gaagatggaa caattagttc tacaggtggt agtgggactg gtggtgt
//
LOCUS       DASJTC010000224         1062 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.230, whole genome shotgun sequence.
ACCESSION   DASJTC010000224 DASJTC010000000
VERSION     DASJTC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.230"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(38..994)
                     /locus_tag="U4B19_002868"
     CDS             complement(38..994)
                     /locus_tag="U4B19_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078517.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          345 a          177 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt ca
//
LOCUS       DASJTC010000225         1040 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.231, whole genome shotgun sequence.
ACCESSION   DASJTC010000225 DASJTC010000000
VERSION     DASJTC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.231"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            54..989
                     /locus_tag="U4B19_002869"
     CDS             54..989
                     /locus_tag="U4B19_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078518.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          398 a          122 c          185 g          335 t
ORIGIN      
        1 aatataggta caataactat ttgatgctaa atataattaa tggaggagag atagtgaaca
       61 tacttaatga aaaaagatat gtatggtttg ctggtaaaaa atatattact actttcaaaa
      121 gattatccga gttaatgcag cagactgtta aaagtgctat gcctgaagga tacaagttta
      181 attttgttca atcttcagta tctactgaaa gtatctattt ttcaattctt tctgagagta
      241 ggctttttca ttttagaata agttctcatg taaagaaaac tgatgttgat atatatacgt
      301 tccgtacaaa taattataaa aatttaaatg atttgagtag aagtatcagt gaatatctca
      361 aggggaatag ctatctcaag attcgatttt cacatatatt aattttacta ccatatttga
      421 atgagaattt caccctaatt caagtagata agtctttcat tgcctcaggt aatcaaggaa
      481 atatgaatat tacaagtaac aaaacgtcac gtgtgctatt ggcgttgatg ggttacggaa
      541 ttctttatag agatgaccaa aatggccaga ttctaataaa tcgggctggg cgttcattat
      601 taatgaaata tagaaaattt ataccggaag attttcaaaa cttacagtta gaaaacatgc
      661 gtttagaaga tttatttaga ttagcagaag atgataagta tagatttgaa gttttgaaaa
      721 ttcaaaagaa tgaactattg aataagttat atcctgagaa tgcgataaaa gcacattttg
      781 gtgatgctaa tcatatgtat agttggttca ttcctaaaag atttagaggg aaaatcaatg
      841 ctggagatca agtgaaagta ctcaatcgaa atagtacaga tgtcgtaatg ataacagata
      901 tctatgtaga agatgaagat ataatcaaat atatgaagcc tgtgataaaa cttgttaata
      961 aaaataaagg aacccgtggt gctagttaat tttttcctag agaaaaatgt ctaaacatgt
     1021 tggtaaatca acaaaaaaac
//
LOCUS       DASJTC010000226         1023 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.232, whole genome shotgun sequence.
ACCESSION   DASJTC010000226 DASJTC010000000
VERSION     DASJTC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.232"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            369..1004
                     /locus_tag="U4B19_002870"
                     /pseudo
     CDS             369..1004
                     /locus_tag="U4B19_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          350 a          183 c          185 g          305 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac cttatattat ttgtcaaatt
     1021 aag
//
LOCUS       DASJTC010000227         1002 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.233, whole genome shotgun sequence.
ACCESSION   DASJTC010000227 DASJTC010000000
VERSION     DASJTC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.233"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(313..903)
                     /locus_tag="U4B19_002871"
     CDS             complement(313..903)
                     /locus_tag="U4B19_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEN2078519.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT          309 a          181 c          173 g          339 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta ag
//
LOCUS       DASJTC010000228          995 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.234, whole genome shotgun sequence.
ACCESSION   DASJTC010000228 DASJTC010000000
VERSION     DASJTC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.234"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..549
                     /locus_tag="U4B19_002872"
                     /pseudo
     CDS             1..549
                     /locus_tag="U4B19_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            607..>995
                     /locus_tag="U4B19_002873"
     CDS             607..>995
                     /locus_tag="U4B19_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078520.1"
                     /translation="MIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLSFGIG
                     LTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQAPWRF
                     MIFSTLFISFSVSLFISPQLEKFSYTR"
BASE COUNT          311 a          144 c          135 g          405 t
ORIGIN      
        1 ttttatgata cggcatttca cattaatcgt gcgttaagtt tagaatctat ttttagctcc
       61 cctattaatt ttgaaacgtt tagatcatat ggcatgcaag taaataattt ttacccatgg
      121 ttaactctat atcctttatt tttattgatc aagtttacta atttagcaat aggctataac
      181 ctttttcttt atattgttac gctcattact ctatttatat gtcattatgt aatgtatgaa
      241 attaccaaaa aacatgttac tagctccttt ttcgctatta tatatacaac atcttctttt
      301 aggagtgtag aaatatttct tcgaggggca atgggggaat tactcgcaat gtcaatactt
      361 cctcttattt tactcgggtt tataaagctt tacgactcaa aaaaagaatc ttgggttatg
      421 ttggcgatta gcatgacttt attaatttac actcatgttc tttctgtagc tatgactatg
      481 gtaatgatta taattgcttt aattattcaa ttttatcgga aaaagaaaat agaaatcttc
      541 ttaattatta aattaataaa agctgctata gttactttac ttctttcact agcgtttata
      601 ggtcctatga ttgagcagac attgtatcaa gatctgaata gaccttatgt ggatatatta
      661 caaaaaagag caatttatct gggtaaagag tttttgattg gaagtataga ttcagaatta
      721 ctatcatttg gtattggctt aactttgctt atttgtttaa ttatactaac atttaacttt
      781 aaaaaattag gaatcttatc taaaatcact tatataatgg gtataatatc tattctactt
      841 tgtacaaaaa tatttccatg gtttgcctta caaaatactc ctttaaatat tattcaagct
      901 ccttggcgtt ttatgatatt ttcaacattg ttcatatctt tttcagtatc tctatttatt
      961 tctccacagt tggaaaaatt ttcttataca aggag
//
LOCUS       DASJTC010000229          987 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.235, whole genome shotgun sequence.
ACCESSION   DASJTC010000229 DASJTC010000000
VERSION     DASJTC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.235"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>987
                     /locus_tag="U4B19_002874"
     CDS             <1..>987
                     /locus_tag="U4B19_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEN2078521.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKMF"
BASE COUNT          375 a          159 c          189 g          264 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgttt
//
LOCUS       DASJTC010000230          975 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.236, whole genome shotgun sequence.
ACCESSION   DASJTC010000230 DASJTC010000000
VERSION     DASJTC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.236"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>975)
                     /locus_tag="U4B19_002875"
     CDS             complement(<1..>975)
                     /locus_tag="U4B19_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEN2078522.1"
                     /translation="LISYHVPTGTPGLLARQGFIEHRLFGVYTSPNVGAMFGYTSIIL
                     SLISVLISKITKTIRVFYMMNFIVQIFYITLSSSRGAQVVICSFLVLFLLLFANSKFR
                     KSIREVLPVKIKWMVSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKT
                     PLPEKEVILQHSSDNSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKV
                     DLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNY
                     AFLGIVLIFILSIFIDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKK"
BASE COUNT          377 a          169 c          120 g          309 t
ORIGIN      
        1 cttttttaaa tacaagtaag aaatgcttcc caaataatac cagaaaacaa ttgaaatgac
       61 atcgcgctta ttgaaaagta catgagcctc caccatttca tctataaaaa tagatagtat
      121 aaaaattaaa acaataccaa gaaacgcata attcacttta cttctaaaaa atgttttgag
      181 taaactaaag atatttaaca catagaaagc atagatacat ataaaagcaa ttataccact
      241 tgatacaagt acttgaagaa atccattatg catgtttcca tgtgctcttt ttagttcatt
      301 cacatctgta gtatcaagat ctactttttt caaaaatttt gcatcgctat tcccatagaa
      361 atcggtctct ccataaccaa ataacaaagt tggtcttata actttaagcg cagctttcca
      421 aatcgtaaaa cgaccagctg aaatttcgga gttatcacta gaatgctgta atattacttc
      481 tttttctgga agtggagttt tatctgattc atttgactct ataacttcta cttttctatc
      541 aatcgataca ggaattgttg ctaaaaaatc cttagcataa ttagtaccaa aagaaaaata
      601 taccaataga actaataata tgcttaccat ccatttgatt tttacaggta gtacttctct
      661 aatgctcttt ctaaattttg aattggcaaa caacaaaaga aataatacta aaaaagaaca
      721 aattactacc tgggcacctc tagacgaaga aagtgtaata taaaatattt gtacgataaa
      781 attcatcata taaaaaacac gaatcgtttt tgttattttt gaaattaata cactaattaa
      841 acttaaaata atagaagtat atccaaacat agctccaaca tttggacttg tatagacacc
      901 aaataaccga tgttcaataa aaccttgtct tgcaagtaat cctggcgttc ctgttgggac
      961 atgataagaa atcaa
//
LOCUS       DASJTC010000231          948 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.237, whole genome shotgun sequence.
ACCESSION   DASJTC010000231 DASJTC010000000
VERSION     DASJTC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.237"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..460
                     /locus_tag="U4B19_002876"
     CDS             <1..460
                     /locus_tag="U4B19_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEN2078523.1"
                     /translation="LELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIKMAIAS
                     SSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEECLVIED
                     SYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(558..>948)
                     /locus_tag="U4B19_002877"
     CDS             complement(558..>948)
                     /locus_tag="U4B19_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HEN2078524.1"
                     /translation="KRLMWEDGAPMGIDNFFASEEKFPTLFEKGSQYTSLYKLLEDDY
                     DVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQPFHYSQSTVRCDR
                     ISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT          339 a          131 c          154 g          324 t
ORIGIN      
        1 tttagaattg aagggaaaat ttactaaata ttggaaatcg caaaagattg attacttgga
       61 tatttttcct aatatactaa ttgaacagct tgaatggtta aaaaagaaaa aaataaaaat
      121 ggcaattgct tcttccagtc caatggatac tataaatgaa gttgttacca cttgtaaaat
      181 agagtcatat tttgattgtt taattagcgg aagagatttg ccagaaagta aacctaatcc
      241 tactatattc cttaaagctt cagaacagct cagaattcca gttgaagaat gtctggtgat
      301 agaagattct tataatggta taaaagctgg taaaagagca aatatgaagg ttcttgcaat
      361 taaagataaa cgtttttctc aagatgtaag tctagcagat gatgttattt acgatattaa
      421 atatctgcga caagaagtac aggtgaaatt tggaatataa ataagttttt tgatgtattt
      481 agagaatatt agtaaaaata aaaatcatgc ccaaatctaa tcaaaattga ttgctttggg
      541 tatgatttct tttaatatca atctattact ttaggatctg aatcattttt atgcaccgta
      601 tctaaagtga tattttctgt gacttcaata acataactaa ttctatcaca acgaactgtg
      661 gattgagaat aatgaaatgg ttgattattt ttatctttgc caaccttttc taaaataaat
      721 agcggatctc ctactataca ttgcaataag tcggaaagct caggtgttgc agataaaccg
      781 tttatttcca taatagagct cgtagctttg acatcatagt catcttctag aagtttatat
      841 aatgaagtat attggctacc tttttcaaat aaagttggaa atttttcttc tgatgcgaaa
      901 aaattatcaa ttcccatcgg ggcaccgtct tcccacatta aacgttta
//
LOCUS       DASJTC010000232          946 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.238, whole genome shotgun sequence.
ACCESSION   DASJTC010000232 DASJTC010000000
VERSION     DASJTC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.238"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            28..101
                     /locus_tag="U4B19_002878"
     tRNA            28..101
                     /locus_tag="U4B19_002878"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62..64,aa:Ile,seq:cat)
     gene            complement(616..915)
                     /locus_tag="U4B19_002879"
     CDS             complement(616..915)
                     /locus_tag="U4B19_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078525.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT          312 a          156 c          149 g          329 t
ORIGIN      
        1 gtttatggct ttatgtggca acaaccagta cccttagctc agttggtcag agcagacggc
       61 tcataaccgt ccggtcgtag gttcgagtcc tacagggtac attaacgtag ccatttgaat
      121 cgttctgtgt tagaattttt tgaagagtat tatacaagct aaagcttttc ttcattgcca
      181 ctcaaatgag tggctttttt atgtatcctt ttatggatta atgaaaggat gtttcacata
      241 gttatacttc tgtatatttg aaaagtttta ctttgatttt taaatagaaa gacatttggg
      301 ttatattgtg agataataat aaagaagagt ttaaagcgtt ccccaaaaac cactgcccca
      361 taagtgtgtt acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt
      421 ttgaaaaaaa gtgagaaaaa aggcttataa ttggagtggt agttaattag tgacttattt
      481 ttgattttat agcactgata ctataaaata tagatatcat catattacac aatcttaata
      541 ctaacttaaa aaatatctcc tttcataagt atggtgataa aatccgttcc gggctacctt
      601 tttaggtagc ctactttaat ctttgtatct ttctggatca acgaaagtat actttatata
      661 gtcataacgc cgatgatcgc ttcgagcgtc tagcacatca gtcacgatat caaacaaaaa
      721 atatacgtct ttcttcattc tagttttcgc agcagggatt ttaaagtagt tcttattaga
      781 atagtagaga ttaattaata agctatcttc gattgctaaa aagaaaactt ctgaatccca
      841 caccttataa aaatctttga caaatctatt cgaaggatca aatttaaacc ataattgcgt
      901 ctcattaaaa agcataacca ttactccgat ctatttttaa acttag
//
LOCUS       DASJTC010000233          943 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.239, whole genome shotgun sequence.
ACCESSION   DASJTC010000233 DASJTC010000000
VERSION     DASJTC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.239"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>943
                     /locus_tag="U4B19_002880"
     rRNA            <1..>943
                     /locus_tag="U4B19_002880"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DASJTC010000234          923 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.240, whole genome shotgun sequence.
ACCESSION   DASJTC010000234 DASJTC010000000
VERSION     DASJTC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.240"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..>923
                     /gene="bsh"
                     /locus_tag="U4B19_002881"
     CDS             6..>923
                     /gene="bsh"
                     /locus_tag="U4B19_002881"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEN2078526.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDS"
BASE COUNT          319 a          124 c          167 g          313 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agt
//
LOCUS       DASJTC010000235          917 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.241, whole genome shotgun sequence.
ACCESSION   DASJTC010000235 DASJTC010000000
VERSION     DASJTC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.241"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>917
                     /locus_tag="U4B19_002882"
     CDS             <1..>917
                     /locus_tag="U4B19_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEN2078527.1"
                     /translation="VSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIV
                     FLLIGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFI
                     STFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWR
                     YALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNN
                     AWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSL
                     VVPITLGYFFQFLYSLPVNAEFYEKKTSF"
BASE COUNT          276 a          114 c          155 g          372 t
ORIGIN      
        1 ttgtatcaat tttttcgatt cttgttgggc taagcctaaa ttcggcaatt aataatgcaa
       61 gttttgacta taaagatgac ataaatgaat ttatgtcatc ggtactttct ctagctacac
      121 tttcgttaat tgtgttctta ttaataggga acatatattt tttgttttca aatgcttttt
      181 ttgatatgaa ccaacttgta tttaatttga tgttattaca aagttttgct agttttgtaa
      241 ttagttatat ccagtcttat tttacgatta actttaacca ttttaaatat ttgattttat
      301 cttttatatc tacttttttg aatattgcaa tctctgtagc tttgatgcag acattatatt
      361 ttgatgataa atatgttggc agagtagtcg gcggaacact gtcatttgtg ttgataggtt
      421 tagtaattta tttaagaata atgataaagg gtgctcattt ttttgataaa aaacagtgga
      481 gatacgcttt aaaaatctct ttgccgctaa tcccacattc cttagcaaat atagttttag
      541 cacaattcga tagaattatg attaattctt atttaggtcc tgctgcgggg ggaatatatt
      601 cctacattag taacataggt attattctaa gcgtaatatg gatgtctact aataacgcct
      661 gggttccatg gttctatggt gagatgagta aaaataatga aaaaaaagtg aagaaagtta
      721 gtaactatta tttagtagtc tttactttga ttacgatgat tgtaatggta gtttcgattg
      781 atttagcaaa atttatggct ccgaaagact attattctgg gttatcttta gtggttccga
      841 taaccttagg ctatttcttt cagtttttat atagtttacc agttaatgct gaattttatg
      901 agaaaaaaac atcgttt
//
LOCUS       DASJTC010000236          911 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.242, whole genome shotgun sequence.
ACCESSION   DASJTC010000236 DASJTC010000000
VERSION     DASJTC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.242"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="U4B19_002883"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="U4B19_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN2078528.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          260 a          190 c          138 g          323 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaattat acagaaacac tttattcaaa ataggaaaga
      841 tcaggaaagc catcaattgg aggaaaaaac tctgtttgtt catcatgata tagattatct
      901 tttgctttaa a
//
LOCUS       DASJTC010000237          872 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.244, whole genome shotgun sequence.
ACCESSION   DASJTC010000237 DASJTC010000000
VERSION     DASJTC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.244"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            94..>872
                     /locus_tag="U4B19_002884"
     CDS             94..>872
                     /locus_tag="U4B19_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEN2078529.1"
                     /translation="MHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQ
                     GYVSWGAGNANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTS
                     LFTKGIISHLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPV
                     IDPTRAGAANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKE
                     LARVRADGIYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYV"
BASE COUNT          295 a          149 c          178 g          250 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gt
//
LOCUS       DASJTC010000238          866 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.245, whole genome shotgun sequence.
ACCESSION   DASJTC010000238 DASJTC010000000
VERSION     DASJTC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.245"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            4..>866
                     /locus_tag="U4B19_002885"
     CDS             4..>866
                     /locus_tag="U4B19_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEN2078530.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYV"
BASE COUNT          294 a          157 c          178 g          237 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgt
//
LOCUS       DASJTC010000239          854 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.246, whole genome shotgun sequence.
ACCESSION   DASJTC010000239 DASJTC010000000
VERSION     DASJTC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.246"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..834
                     /locus_tag="U4B19_002886"
     CDS             <1..834
                     /locus_tag="U4B19_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEN2078531.1"
                     /translation="FSIVHNQVYFGGGINLWLAKKNGIRQRIAHSHATEDGKSQNIVM
                     NVLRKFLTKLLLQNATDYLAVSQEAGESLFQNHPFEIVHNGIDLELYSKNSEAKVNKR
                     KELDISMSTFVVGNIGRLEKQKNQSYLLEIFLEILKMNSDSYLILVGEGSLRNKLKAK
                     AVELDIEKRVKFLGERNDIPELLSVMDVFVMPSLYEGLPMVGLEAQAAKLKLVLADTI
                     SKDTKLTNNVDFVSLDETPQEWAKKVIKEPYANKITQELKSYDVSYTTEQMESIYSRD
                     H"
BASE COUNT          329 a          102 c          164 g          259 t
ORIGIN      
        1 ttttctatag ttcacaatca ggtttatttt ggcgggggaa ttaatttatg gttagccaaa
       61 aagaatggaa tcagacagag aattgctcat agccatgcaa ctgaagatgg aaaatctcaa
      121 aatattgtaa tgaatgtatt gcgtaaattt ttaaccaaat tgttgttaca aaatgcaaca
      181 gactaccttg ccgttagtca agaagctggt gaaagtctgt ttcaaaacca cccctttgag
      241 atagttcata atggtattga tttagagctt tatagtaaaa attcagaagc taaggttaat
      301 aagagaaaag aattggatat ctcaatgagt acttttgttg tgggaaacat tggacgatta
      361 gagaaacaaa aaaatcaatc ttatttgtta gagatttttt tagaaatact aaaaatgaat
      421 tctgattctt acttgatttt agtaggtgaa gggtcactta gaaataaact gaaagccaaa
      481 gcagtagaat tggatataga gaaaagagta aaatttttag gagaaagaaa cgatattccc
      541 gagttattat ctgtgatgga tgtttttgta atgccttctc tttatgaagg tctgccaatg
      601 gttggattag aagcacaagc agcaaaatta aaattagtct tagcagatac tatttccaaa
      661 gatacaaagt taacaaataa tgtggatttt gtatcattag atgaaacacc acaagaatgg
      721 gcaaaaaaag taataaaaga gccgtatgct aataaaataa cacaagaact aaagagttat
      781 gatgtttcgt acactactga acaaatggag agtatatata gtagagatca ttaaaaatgt
      841 tcaagatatt ttag
//
LOCUS       DASJTC010000240          852 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.247, whole genome shotgun sequence.
ACCESSION   DASJTC010000240 DASJTC010000000
VERSION     DASJTC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.247"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..704)
                     /locus_tag="U4B19_002887"
     CDS             complement(<1..704)
                     /locus_tag="U4B19_002887"
                     /inference="COORDINATES: protein motif:HMM:NF026247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="HEN2078532.1"
                     /translation="MDTEASLEINQEKSVSQKKWSTSNKILSGVLLLIMWIIMAFNTQ
                     NADYYAYSVLYNGIAQFGMDFSSDSIEPGFRAVMYVCTFISSQYQIFLLIISTVLLSA
                     LLYVIRLFTTNVFLVLFLFFIFGFTSQAIQVRMFSALIFILLAFYHLTKLRGSKAIFK
                     YIFFILVAGLFHNLAYFYLPFFIVKINKKITGYVTILSLLLFSIGQSYISSIFINTKY
                     DAYFDRNSTSISHVVM"
BASE COUNT          327 a          150 c          116 g          259 t
ORIGIN      
        1 aacatcacta cgtgagaaat cgatgtactg tttctatcaa aatatgcatc atacttcgta
       61 tttataaata ttgaactgat ataactttgt ccaatagaga ataggagcaa gctaaggatt
      121 gtaacatatc cagtaatctt tttattaatt tttacaataa aaaaagggag ataaaaatat
      181 gcaagattat ggaacagtcc agccactaag ataaaaaaaa tatacttaaa tatagctttg
      241 ctgcctcgca atttagttag atgataaaaa gctagtaata tgaatatcaa tgcgctaaac
      301 atacgtactt gtattgcttg tgaagtaaaa ccaaaaataa aaaacaagaa caaaactaaa
      361 aaaacattag ttgtgaataa tctgatgaca tacaataaag ctgagagtaa gacagtagaa
      421 ataatcaata aaaaaatctg atactgactg gaaataaagg tacatacgta cataacagct
      481 ctgaatcccg gttcaatact atctgaacta aagtccatac caaattgtgc tatcccatta
      541 tacaagacag aatatgcata ataatcagcg ttttgtgtgt taaatgccat gatgatccac
      601 attatcaaaa gtagcacccc agacaaaatc ttgttcgatg tactccattt tttttgtgaa
      661 acactctttt cttgattaat ctccaatgac gcctctgtat ccatctcgaa ccccccttat
      721 agccgctcta aatctactga gcttatcagt ctcaaaagct attgcaataa aaaatttcaa
      781 taatctttta ttcataaggc gggaaaattc cttatcactt ttatacattc ttttcaccaa
      841 tgttgaattt ct
//
LOCUS       DASJTC010000241          843 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.248, whole genome shotgun sequence.
ACCESSION   DASJTC010000241 DASJTC010000000
VERSION     DASJTC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.248"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>843)
                     /locus_tag="U4B19_002888"
     rRNA            complement(<1..>843)
                     /locus_tag="U4B19_002888"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          184 a          259 c          187 g          213 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gct
//
LOCUS       DASJTC010000242          839 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.249, whole genome shotgun sequence.
ACCESSION   DASJTC010000242 DASJTC010000000
VERSION     DASJTC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.249"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            68..643
                     /locus_tag="U4B19_002889"
     CDS             68..643
                     /locus_tag="U4B19_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEN2078533.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(712..>839)
                     /locus_tag="U4B19_002890"
     CDS             complement(712..>839)
                     /locus_tag="U4B19_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEN2078534.1"
                     /translation="VNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
BASE COUNT          234 a          143 c          178 g          284 t
ORIGIN      
        1 gttcttttaa atgcacaaga agaaacgatt atgaaagatt tggaaaggtt aaagatgggc
       61 taacaaaatg aataaaaaca aaatggcaac tcgcgtactt acagtgatgg ctttgtgtat
      121 tgggatcaat tatattggag gaacgattgc tttatggctt cgtttgccaa tctatctcga
      181 ttctatcgga acgatttttg ctggtgcatt gcttgggcct gtacctggaa tgctgacagg
      241 attgtccagc ggtattttaa gtggagtgac tacagatatt ttttctctgt attattcgcc
      301 aatccaaatt ctcacaggct tgcttgctgg attgattttg ccaagatatc tcggcaaaga
      361 taaaaacagc ttgaaactgt tattactcgc tttgattttg tcggtacctg gaacgatcgt
      421 ttcttcgatc attaccgttc agttatttgg cgggatcacc tcctcaggat ccagtatgat
      481 cgttcagcta ttacatgggt taggtttaag tcagacaaca agtgtgatct tggtacaggc
      541 gggtacagac taccttgatc gcttgttgtc tatcgtcgtt gtttcattag tcatcactat
      601 gttgccccaa cgcttgatat attcaaaaag aaaaataggc tgaataaaca gagctggatt
      661 agcaaaataa tgctaatctg gctctgtttt ttcattttat tatttgtcaa attaagcgag
      721 acaaaacttt ccatttacgt aactcaaaaa aacttctaat ggtgtttggt aatttagtga
      781 ttttctaggg atatggtttc ttttggacgc aacggatgag atgaatcctt gattgactt
//
LOCUS       DASJTC010000243          805 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.250, whole genome shotgun sequence.
ACCESSION   DASJTC010000243 DASJTC010000000
VERSION     DASJTC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.250"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            107..>805
                     /locus_tag="U4B19_002891"
     CDS             107..>805
                     /locus_tag="U4B19_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEN2078535.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGI"
BASE COUNT          281 a          117 c          140 g          267 t
ORIGIN      
        1 tagcgtaaaa tattttgtgt aaatagatat ttagggtaga aaaaaagaaa gtccattctg
       61 taaaattaaa gttaccacaa ccgaaatttt ataggaggac tttctcatgc ccaattttac
      121 tacagaaatt atggaaacac taatcaataa aggtgattta gatgaattat ttcgatgtca
      181 tttagaatta gctgttaatt cattattgca ggctgaatta acagcttttc ttgattatga
      241 aaaatatgat cgtgctgggt tcaattcagg taattcccgt aatgggaatt attcacgttc
      301 attcagaaca gagtatggag aattaaattt ggtaattcct agagatagaa atggtaagtt
      361 ttctcaacaa actttacctg cttataaaag aaccaatgac tctttagaaa ccactattat
      421 tcagctattt caaaaaggaa ttactatgtc tgaaatctct gaattgattg aaaaaatgta
      481 cgggcatcac tatacgccac aaaccatttc aaatatgact aaaatagttt ctgaagatat
      541 tattgccttt aaagaaaggt ccttagaatc cagatactct gtcattttta tggatgctac
      601 tcacattcct ttaaaaagac aaactgtttc aaaagaagca gtttatattg taattggcat
      661 tcgattagat ggaaccaaag aggttcttgg gtttagtctt gctccaactg aatctgctta
      721 tgtttggaaa gagattcttc aagacctaaa agaccgtggt ttaaaagagg ttttattagt
      781 cgtaactgat ggtttaagtg gcatt
//
LOCUS       DASJTC010000244          757 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.251, whole genome shotgun sequence.
ACCESSION   DASJTC010000244 DASJTC010000000
VERSION     DASJTC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.251"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            199..>757
                     /locus_tag="U4B19_002892"
     CDS             199..>757
                     /locus_tag="U4B19_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEN2078536.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDR"
BASE COUNT          301 a           77 c          141 g          238 t
ORIGIN      
        1 tagtagaaaa tcgccttcga ttttccaaca acacaaaata ttatagaacc ctttattcta
       61 aaggatttca aggtgtctgt ttgcatttat tagaaaaaag aaaaagtatt ggcaacagaa
      121 ggtgtcacca acactagata ttatagagcc ataatatgct gtataatatc tagtgtatag
      181 gcttaagtgg aggtatatat gaaaatatta actgttgaag atgataatat gataagagag
      241 ggaataagtg aatatctttc agaatttgga tatactgtta ttcaagctaa agatggaaga
      301 gaagccttgt caaaatttaa tagcgatata aatttggtta tcttagacat tcagatacct
      361 tttataaatg gtttagaagt gttgaaagaa attagaaaga aaagcaattt accaattcta
      421 atcttgactg catttagtga tgaagaatat aaaattgatg catttactaa tttagtagac
      481 ggatatgtag aaaagccatt ttcattgccg gtcttaaaag ctagaataga ttctttaatt
      541 aagaagaatt atggacattt agagaagttt gaatataagg atctatcggt taattttaat
      601 agctatacag ctaaaataaa tggtgaagaa atagatgtaa atgcaaaaga actagaagta
      661 ttaaaatgtt tattggataa taatggacaa gtgttaacaa gaatgcaaat tattgattat
      721 gtatggaaag atagcgaaga aactccttat gatcgag
//
LOCUS       DASJTC010000245          672 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.252, whole genome shotgun sequence.
ACCESSION   DASJTC010000245 DASJTC010000000
VERSION     DASJTC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.252"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(233..532)
                     /locus_tag="U4B19_002893"
     CDS             complement(233..532)
                     /locus_tag="U4B19_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEN2078537.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          226 a          117 c           76 g          253 t
ORIGIN      
        1 acaaaaatgc aacattaata aataacgata gtttcataaa atacactttt aattaacatt
       61 attagtttat actagctgtt tttgtgttaa ttaaaggata aaatacaata taaagaaaca
      121 tctgtttctt tatttagaat aaaaaaatac agactctgtt caaataaatg aacctagcct
      181 gcattatgag attgactata ctttttaatt cattgagatg aataactcgt ttttataaat
      241 gcccaaaagt aatcggtgcc gaagccattc ctcgacctaa ttctttttca aattttccta
      301 ttgttgatat ctccatataa aacggtgata ctggtttaga caattctccc ctgattgcta
      361 acggacgtaa actacgttcc aaattattta tgactttagg ataatactga cctttttcta
      421 agcctgtctt tgcgtctatc aaaagtatac gttccttttc tttgatctct ggatttaaaa
      481 cgagatcata taattttagt accatgtttt ctatttcttc acttttagac atcacgattt
      541 cctcccaaaa atatatttct tcccactaca ttctataaac aaagtatacg ctacatctag
      601 atttttttaa atcattactg gattgttaaa acctttcaat acactgttat ggcaacgatt
      661 tttattttaa aa
//
LOCUS       DASJTC010000246          650 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.253, whole genome shotgun sequence.
ACCESSION   DASJTC010000246 DASJTC010000000
VERSION     DASJTC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.253"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            6..638
                     /locus_tag="U4B19_002894"
     CDS             6..638
                     /locus_tag="U4B19_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEN2078538.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT          235 a           92 c          101 g          222 t
ORIGIN      
        1 taaatatgat ttttcctaaa gttaatgaag caagcttgaa taaaggatcc ttttttcata
       61 tctatgaaaa cgaagatttt gaactgtata atatcctttc acaaaaagag gtcatcgaaa
      121 accaaataat ctgccatcca attatttaca aatctatttt caagtatgca ttttttgctt
      181 ccaatgattt tcaaagaatg gagattaccg actgtatatt cgaaaactgt gatttttcag
      241 gctgtatatt gacaaaaagc atgttgcatc gcgtgaaatt tgtcaattgt aaattaacag
      301 gtactaaatt gatggaaagt tatattggga acacgttatt cgagaattgt aaaatggact
      361 atgtaaattt atctggttca aacataaaag aaagtacctt tgagcactct gtcttatcaa
      421 gcgcagattt tgtagactgt tccctcacta aaataatgtt ttctactaat gatttagaaa
      481 atgctagctt ttttgatact gatttaaaaa atatcgattt aagtagcaac tcctttgaaa
      541 tgattgaagt acgctcggaa catctaaaaa attgtacgct taatcagttg caagcacttg
      601 gttttgctag aaaattttta caaataaaag tagtataatc aaaatgaata
//
LOCUS       DASJTC010000247          638 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.255, whole genome shotgun sequence.
ACCESSION   DASJTC010000247 DASJTC010000000
VERSION     DASJTC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.255"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          221 a           98 c          103 g          216 t
ORIGIN      
        1 gaagagtatt atacaagcta aagcttttct tcattgccac tcaaatgagt ggctttttta
       61 tgtatccttt tatggattaa tgaaaggatg tttcacatag ttatacttct gtatatttga
      121 aaagttttac tttgattttt aaatagaaag acatttgggt taaattgtga gataataata
      181 aagaagagtt taaagcgcac cccaaaccac ttccccataa gtgtgttacg ctttaaactc
      241 ttttatattt gaagccatta aaaagcatac catatttttg aaaaaaagtg agaaaaaagg
      301 cttacaattg gagtggtagt taattagtga cttatttttg attttatagc actgatacta
      361 taaaatatag atatcatcat attacacaat cttaatacta acttaaaaaa tatctccttt
      421 cacaagtatg gtgataaaat tcgttccggg ctaccttttt aggtagccta ctttaatctt
      481 tatacctttc tggatcaacg aaagtatact ttatatagtc ataacgccga tgatcgctac
      541 gtgcgtccgg cacgtcagtc atgaatcggc tacactagac tagacagaaa aaataaggtg
      601 tgtagaataa taaaaaacac actggaggat ttttcatg
//
LOCUS       DASJTC010000248          633 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.256, whole genome shotgun sequence.
ACCESSION   DASJTC010000248 DASJTC010000000
VERSION     DASJTC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.256"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            330..>633
                     /locus_tag="U4B19_002895"
     CDS             330..>633
                     /locus_tag="U4B19_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEN2078539.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTK"
BASE COUNT          234 a           71 c          104 g          224 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaa
//
LOCUS       DASJTC010000249          628 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.257, whole genome shotgun sequence.
ACCESSION   DASJTC010000249 DASJTC010000000
VERSION     DASJTC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.257"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            98..433
                     /locus_tag="U4B19_002896"
                     /pseudo
     CDS             98..433
                     /locus_tag="U4B19_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          214 a           69 c          109 g          236 t
ORIGIN      
        1 aggttgcgaa ggaagatgta gagttatttg cactgttgtg ttggtatatc tcttaatgct
       61 gtgtcttttt ggggagatta attagtggag tgattatatg agtatagatg attgtttaga
      121 agaacaaata agattatttt taaagttttt aaaagttaaa gttcaaaata actacttcac
      181 atgttatagt tatgcacacc ctgttttaaa tagtcttcct tttaattctt tagtgcaagg
      241 attgattaca aatattgttt tattagtctg tacaccaaat gaaatatttg taaaaaagat
      301 aggcagtggt atttcttcag taagattaaa agagaaagaa tatactcaaa agattattca
      361 tattcctaag cctgaaatta agcgttttga gataaaagac tattctgctt tagggctgtt
      421 ctcaattggt tactggtgat aaaaaataag aatactaaaa ctatacgtag aatataagta
      481 atcggcatca atcctaatta tgtgattgaa tgccgatttt tgttacgcgc tatttttaat
      541 tatctgtaag aagaataatt ttattttact catattcgtt ttcactttga agaagtaaat
      601 tgcaatttct tataagttaa gcagctat
//
LOCUS       DASJTC010000250          604 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.261, whole genome shotgun sequence.
ACCESSION   DASJTC010000250 DASJTC010000000
VERSION     DASJTC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.261"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..168
                     /locus_tag="U4B19_002897"
     CDS             <1..168
                     /locus_tag="U4B19_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEN2078540.1"
                     /translation="NKISFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYH
                     ANEVLKIAEEK"
     gene            169..>604
                     /locus_tag="U4B19_002898"
     CDS             169..>604
                     /locus_tag="U4B19_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEN2078541.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSA"
BASE COUNT          233 a           70 c          113 g          188 t
ORIGIN      
        1 aataaaataa gttttgaaga aaacattgtt aaagaaatgc ctgaatctgc taatgatatg
       61 tatgagaagg agttgaaata tttctttgaa aacgtagtaa caggcaaaga aaactggaat
      121 gatttatatc atgcaaacga agtattgaaa atagctgagg agaaataaat ggaaatttta
      181 tttactattt gtggtagagc tgggtctaag gggtttaaaa ataaaaattt gaaatatttg
      241 gcaggaaagc ccctagtcca tttcgcttta gcaatgatag atatctatca acaaaaacat
      301 cctgaacata attgcacgac ttgtttgaat acggacagta atgagttagt agaattgacg
      361 actaagttaa atccgtctgt gaaaattgta gaacgtaaac ctgaattagc tacagatact
      421 gtagggaaaa ttgaagtgat acgtgattca ttcttacaac tagaaagaga agataatata
      481 tttgatttaa ttatagattt agatttgact tcacctttac gtgaagtttc taatcttgaa
      541 gaattaatag atgaacatct aaaaaattta gataaagacg tctacttttc agttgtttct
      601 gcaa
//
LOCUS       DASJTC010000251          599 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.262, whole genome shotgun sequence.
ACCESSION   DASJTC010000251 DASJTC010000000
VERSION     DASJTC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.262"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(54..>599)
                     /locus_tag="U4B19_002899"
     CDS             complement(54..>599)
                     /locus_tag="U4B19_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2078542.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
BASE COUNT          169 a          111 c          106 g          213 t
ORIGIN      
        1 cttttttgga gtggagaaat ccactccttt tttcatgctc taaaatgata tttttacaca
       61 actgttacgg ttgttttacg aaattggacg ggactttgcc agtttaattt ctgttttatc
      121 cgttcattat tgtagtaata gatatagcta tcgatggctg attttaattc ctcgaaacag
      181 ttataaactt tcccgtgaaa aatctcttgt tttaataagc caaaaaaatt ctccataagt
      241 gaattatcta agcagttccc tttccgagac atgctttgaa agattttgta ctccttcaat
      301 ttgtttctat aagctttcat ttgatacgcc caaccttgat ctgagtgaaa cgtacgacga
      361 tagggacaat cgttagtggt ctggatagct tcctccaaac cttccattac cgctaatgca
      421 ttgggctttt cagagatacg ataggataaa atctcactat tgaacatatc cataaaagga
      481 tctagataca atttcttttg acggataatg cctttggcat ccacttcatg gtatttaaat
      541 tcagttgtat ccgtcgtgat tttctgatgg cagatactgg tgtagaaacg acgatggat
//
LOCUS       DASJTC010000252          534 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.267, whole genome shotgun sequence.
ACCESSION   DASJTC010000252 DASJTC010000000
VERSION     DASJTC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.267"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>534
                     /locus_tag="U4B19_002900"
     CDS             <1..>534
                     /locus_tag="U4B19_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEN2078543.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELG"
BASE COUNT          163 a           80 c          137 g          154 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtat
//
LOCUS       DASJTC010000253          462 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.269, whole genome shotgun sequence.
ACCESSION   DASJTC010000253 DASJTC010000000
VERSION     DASJTC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.269"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>462
                     /locus_tag="U4B19_002901"
     CDS             <1..>462
                     /locus_tag="U4B19_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2078544.1"
                     /translation="GKKELASVGMFFSAIVYLILFLIKASNMYVFLILSSVGYMGLGV
                     FNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVGGWTLSIIGYDS
                     LANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENCELLK"
BASE COUNT          143 a           55 c           93 g          171 t
ORIGIN      
        1 tggaaaaaaa gaactggcat cagtaggaat gttcttttca gcaatagtat atttgatctt
       61 atttttgata aaagctagta atatgtatgt attcttaatt cttagttcgg ttggctatat
      121 gggtttgggt gtgtttaata ctgttatttg ggcaaatatt actgatgtta ttgatgatca
      181 agaagtaaag tctgatcaaa gagaagatgg gaccgtatat gcggtatatt cttttgctag
      241 aaaaataggt caagctctag ctggaggcgt cggaggttgg actctttcta ttataggcta
      301 tgactcttta gcaaatgtac aaacgggaga tgttttagaa aagttatata acgcttcaac
      361 attaattcca tcaatttgtt ttctgattgt aggattaatt ttatttttcc tttatccatt
      421 atctaaagat aaagtcttag aaaattgcga gcttttaaaa ca
//
LOCUS       DASJTC010000254          457 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.270, whole genome shotgun sequence.
ACCESSION   DASJTC010000254 DASJTC010000000
VERSION     DASJTC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.270"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>457
                     /locus_tag="U4B19_002902"
     CDS             <1..>457
                     /locus_tag="U4B19_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078545.1"
                     /translation="SIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPI
                     TADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTW
                     KEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          185 a           68 c          106 g           98 t
ORIGIN      
        1 caagtattga aagtggaaca acagtctttg cagtgaaaga cggcgtaagc ttaccagaag
       61 ataaacttcc agtgttgaaa gcaaaagatg gttatacaga tgcgaaatgg ccagaggaag
      121 caactcaacc aattacagca gatgatacag aatttgtatc aagtgcaaca aaactggatg
      181 ataaatctga cgctgacaaa tataatcctg aaggtcaaaa agtgactaca gaattgaata
      241 aggaaccaga cgcatttgag ggaattaaga acaaagaaga tttaccaaaa gatactaagt
      301 atacttggaa agaaaaagta gatgttagcg cagctggaaa taaaaaaggg actgttgtag
      361 tgacatatcc agatggatca tctgatgaag ttgaagtaga tgttacagtg acagacaatc
      421 gttctgacgc cgataaatat gagccaacag tagaagg
//
LOCUS       DASJTC010000255          451 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.271, whole genome shotgun sequence.
ACCESSION   DASJTC010000255 DASJTC010000000
VERSION     DASJTC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.271"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>451
                     /locus_tag="U4B19_002903"
     CDS             <1..>451
                     /locus_tag="U4B19_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2078546.1"
                     /translation="FRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVR
                     TLNDKYVAIFMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNE
                     LLQDIYSRGVHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRN"
BASE COUNT          147 a           89 c           87 g          128 t
ORIGIN      
        1 tcttccgaaa aggtattacc atgtcggaaa tcgcagactt gattgagaaa atgtatgggc
       61 atcactacac gccccaaacc atgtccaaca tgactaaagt gctgactgaa gaagtaaatg
      121 catttaaagt tagaactcta aatgataagt atgtcgctat tttcatggat gctacttata
      181 ttccattaaa acggcaaacc gtctccaaag aagcaatcta tattgccatt ggtatacgag
      241 aagacggcac taaagaagta ctaagttatg cgattgctcc gactgaatcg acctacgttt
      301 ggaatgagtt gttacaggac atttactcca gaggggttca tgatgtctta ctgtttatta
      361 ccgatggctt aaaaggcatg aaagatacga ttcaccagat ttatcctaaa gctaaatacc
      421 aacattgctg tgttcacgtt tcccgtaata t
//
LOCUS       DASJTC010000256          441 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.272, whole genome shotgun sequence.
ACCESSION   DASJTC010000256 DASJTC010000000
VERSION     DASJTC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.272"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..>441)
                     /locus_tag="U4B19_002904"
     CDS             complement(<1..>441)
                     /locus_tag="U4B19_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEN2078547.1"
                     /translation="NYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPNRQLKAN
                     IFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIGDSLNDL
                     SMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILT"
BASE COUNT          149 a           75 c           57 g          160 t
ORIGIN      
        1 atggtaagaa ttctttcaaa tgcttgagat actccgtctt tatcatttga aacagtgaca
       61 taatcagctt ttttcatgat ttcttgatga ctatttccca ttgcaaaact aatatctgca
      121 ctttcaaaca tagataagtc atttaaacta tctccgatcg cgattctcaa aggtttcttt
      181 aaattgtaga ttgattccaa acgatcaaac accacacctt tagatacatt ttgaggtacg
      241 atttcaatat ttacaggtgc cgattcagta acagacacat ttagcatctt tttcaaatgg
      301 ttaaatatat tagctttcaa ttgacgatta ggaataaaaa taaaaaattt gtaaatgctg
      361 atagcatcct tttctataat ttcctcaata ttattataag ggattgaaca aatagatttt
      421 tcatactcaa gctcatagtt t
//
LOCUS       DASJTC010000257          410 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.273, whole genome shotgun sequence.
ACCESSION   DASJTC010000257 DASJTC010000000
VERSION     DASJTC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.273"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..387
                     /locus_tag="U4B19_002905"
     CDS             <1..387
                     /locus_tag="U4B19_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2078548.1"
                     /translation="DRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLI
                     ENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDT
                     YNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          158 a           74 c           65 g          113 t
ORIGIN      
        1 gaccgaaaag aaatctgtga tgactttaag gctgtttatc aagctaactc aaaagaagaa
       61 gcgaatacct tcttatccgg catgattgag aaatggaaga aaaactatcc taaagtgacg
      121 cagtcactca tagaaaacca agacttatta actttttatg attttccacc tagcattcgt
      181 agaaccattt actcaaccaa tctaatcgag tctttcaata agcaaattaa aagatacagc
      241 cgtagaaaag agcagtttca aaatgaagaa tcactagaac gctttctagt cagcattttt
      301 gatacataca atcaaaaatt tctaaacaga agccataaag gttttcaaca ggtaaccgat
      361 acattagttt caatgtttac tgagtaacta attattttgc aggaggacaa
//
LOCUS       DASJTC010000258          395 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.277, whole genome shotgun sequence.
ACCESSION   DASJTC010000258 DASJTC010000000
VERSION     DASJTC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.277"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..143)
                     /locus_tag="U4B19_002906"
     CDS             complement(<1..143)
                     /locus_tag="U4B19_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEN2078549.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KAL"
     gene            complement(162..323)
                     /locus_tag="U4B19_002907"
     CDS             complement(162..323)
                     /locus_tag="U4B19_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEN2078550.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
BASE COUNT          132 a           74 c           42 g          147 t
ORIGIN      
        1 tataaagcct tttctatttc attgcttgat ttcattattg ttaaacaaga atacaagcaa
       61 gaaagaacta tagttaatat gataaaaatt attaaagttc tatttttctt tcttgtaata
      121 tatgctatag cattttttat cactttccta actactcctc tctattttct tactccataa
      181 ctaattgcat ccactggaca agcattttta catcgtccac atctgataca ctccagatga
      241 ttgcaattct caactgaatc tttattcatt tggcaaactt ttttacattt tccacaatta
      301 atacacttat ccttatcctg aatcggctac actagactag acagaaaaaa taaggtgtgt
      361 agaataataa aaaacacact ggaggatttt tcatg
//
LOCUS       DASJTC010000259          375 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.278, whole genome shotgun sequence.
ACCESSION   DASJTC010000259 DASJTC010000000
VERSION     DASJTC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.278"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..132)
                     /locus_tag="U4B19_002908"
     CDS             complement(<1..132)
                     /locus_tag="U4B19_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEN2078551.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DASJTC010000260          374 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.279, whole genome shotgun sequence.
ACCESSION   DASJTC010000260 DASJTC010000000
VERSION     DASJTC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.279"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
BASE COUNT          118 a           51 c           56 g          149 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtatat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctat
//
LOCUS       DASJTC010000261          365 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.280, whole genome shotgun sequence.
ACCESSION   DASJTC010000261 DASJTC010000000
VERSION     DASJTC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.280"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(<1..219)
                     /locus_tag="U4B19_002909"
     CDS             complement(<1..219)
                     /locus_tag="U4B19_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEN2078552.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIK"
BASE COUNT          101 a           80 c           45 g          139 t
ORIGIN      
        1 tttgatcacc cccatgtgat cattcgctct aggactttct tccagcagca acatttcact
       61 tccagcttct tctgttccta aaatggctaa ttcgttttct tctcttttca aatcaaagcc
      121 aacaacgttc ttgtaaaaat caatcatttt atctcggtct ttcacccgaa tggctacact
      181 ttttaaatag gtggcagccc ccaattgaaa atctttcata tttcttgctc ccctctacgt
      241 caagctttat tttaacgatg gattcttctt ctttcaagca ataactttga ttttgttaaa
      301 aaaaatgtga gaaaattcgc attttgcttg ttttttgtaa attaatgagt aaaaactctt
      361 ataaa
//
LOCUS       DASJTC010000262          345 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.282, whole genome shotgun sequence.
ACCESSION   DASJTC010000262 DASJTC010000000
VERSION     DASJTC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.282"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>345
                     /locus_tag="U4B19_002910"
     CDS             <1..>345
                     /locus_tag="U4B19_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEN2078553.1"
                     /translation="AEEFIRSRKAYGSRKIKKALSKRGIQISRRKISRIMKNRGLKSS
                     YTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLF
                     NREILGYSCGEH"
BASE COUNT          129 a           60 c           66 g           90 t
ORIGIN      
        1 cgctgaagaa tttatccgca gccgaaaggc ctacggctca agaaaaataa aaaaagcctt
       61 atcaaaacga ggcattcaga tcagccgacg aaaaattagt agaatcatga aaaatagagg
      121 attaaaatcg agctatactg ttgcttattt taaagtacat cattctactt gcaatgaagc
      181 caaaacgaca aacgtattga atcgtaaatt cttaagagac aacccattag aagcgatcgt
      241 aacagacttg acttatgtac gagtcgggaa aaaatggaat tatgtctgtt tcattttgga
      301 tctgttcaat cgagaaattc tcggctattc ttgtggagaa cataa
//
LOCUS       DASJTC010000263          323 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.286, whole genome shotgun sequence.
ACCESSION   DASJTC010000263 DASJTC010000000
VERSION     DASJTC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.286"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            95..292
                     /locus_tag="U4B19_002911"
     CDS             95..292
                     /locus_tag="U4B19_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEN2078554.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
BASE COUNT          116 a           33 c           68 g          106 t
ORIGIN      
        1 ttattttgct acgtttcaag aaggaattgt tttttgaaat ttaatgaatg ttagaataat
       61 agtctaaaaa cattaattta ggggtgatta gattatgaaa catttaaaaa ttttgtctat
      121 taaagagaca caacttatct atgggggtac cactcatagt ggaaaatatt atggaaatgg
      181 agtgtattgc actaaaaata aatgtacggt cgattgggcc aaggcaacta cttgtattgc
      241 aggaatgtct ataggtggtt ttttaggtgg agcaattcca gggaagtgct aaaatgaaaa
      301 aaaatgctaa gcaaattgtt cat
//
LOCUS       DASJTC010000264          309 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.288, whole genome shotgun sequence.
ACCESSION   DASJTC010000264 DASJTC010000000
VERSION     DASJTC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.288"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            1..309
                     /locus_tag="U4B19_002912"
                     /pseudo
     CDS             1..309
                     /locus_tag="U4B19_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DASJTC010000265          282 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.290, whole genome shotgun sequence.
ACCESSION   DASJTC010000265 DASJTC010000000
VERSION     DASJTC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.290"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            <1..>282
                     /locus_tag="U4B19_002913"
     CDS             <1..>282
                     /locus_tag="U4B19_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEN2078555.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DASJTC010000266          268 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.denovo.291, whole genome shotgun sequence.
ACCESSION   DASJTC010000266 DASJTC010000000
VERSION     DASJTC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.denovo.291"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(36..>268)
                     /locus_tag="U4B19_002914"
     CDS             complement(36..>268)
                     /locus_tag="U4B19_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEN2078556.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           79 a           39 c           47 g          103 t
ORIGIN      
        1 atagaaggac tgaccttctg tctcagattt ttttgttatt tcacttgaat caattgattg
       61 atcaaaaaaa tcctgatttt catgttctca aatgatttaa atccataaga taatcgtttc
      121 agtgtcttga tatggggaat cttggcctct attttgccat tagaatacgg gtaaatcata
      181 gcattgtaaa taccttcttc atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca
      241 tcaagtgttt caggaagatt ttttaata
//
LOCUS       DASJTC010000267        12792 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.guided.078, whole genome shotgun sequence.
ACCESSION   DASJTC010000267 DASJTC010000000
VERSION     DASJTC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.guided.078"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(284..520)
                     /locus_tag="U4B19_002915"
     CDS             complement(284..520)
                     /locus_tag="U4B19_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2078557.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(703..1089)
                     /locus_tag="U4B19_002916"
     CDS             complement(703..1089)
                     /locus_tag="U4B19_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HEN2078558.1"
                     /translation="MDARAMSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECI
                     CVQSISYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAA
                     CSKRMRRRKEAERQRKRYWKPTHLEL"
     gene            complement(1071..2399)
                     /locus_tag="U4B19_002917"
     CDS             complement(1071..2399)
                     /locus_tag="U4B19_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEN2078559.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(2360..2689)
                     /gene="mobC"
                     /locus_tag="U4B19_002918"
     CDS             complement(2360..2689)
                     /gene="mobC"
                     /locus_tag="U4B19_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HEN2078560.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(2947..3317)
                     /locus_tag="U4B19_002919"
                     /pseudo
     CDS             complement(2947..3317)
                     /locus_tag="U4B19_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            3462..3647
                     /locus_tag="U4B19_002920"
     CDS             3462..3647
                     /locus_tag="U4B19_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEN2078561.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            3805..3972
                     /locus_tag="U4B19_002921"
     CDS             3805..3972
                     /locus_tag="U4B19_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078562.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            4590..5249
                     /gene="vanR"
                     /locus_tag="U4B19_002922"
     CDS             4590..5249
                     /gene="vanR"
                     /locus_tag="U4B19_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HEN2078563.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            5252..6613
                     /gene="vanS"
                     /locus_tag="U4B19_002923"
     CDS             5252..6613
                     /gene="vanS"
                     /locus_tag="U4B19_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEN2078564.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(6704..8005)
                     /locus_tag="U4B19_002924"
     CDS             complement(6704..8005)
                     /locus_tag="U4B19_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEN2078565.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            8284..9090
                     /locus_tag="U4B19_002925"
     CDS             8284..9090
                     /locus_tag="U4B19_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HEN2078566.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            9108..9815
                     /locus_tag="U4B19_002926"
     CDS             9108..9815
                     /locus_tag="U4B19_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HEN2078567.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            9812..10783
                     /locus_tag="U4B19_002927"
     CDS             9812..10783
                     /locus_tag="U4B19_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HEN2078568.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            10776..11804
                     /gene="vanB"
                     /locus_tag="U4B19_002928"
     CDS             10776..11804
                     /gene="vanB"
                     /locus_tag="U4B19_002928"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HEN2078569.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            11810..12418
                     /locus_tag="U4B19_002929"
     CDS             11810..12418
                     /locus_tag="U4B19_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HEN2078570.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(12484..12657)
                     /locus_tag="U4B19_002930"
     CDS             complement(12484..12657)
                     /locus_tag="U4B19_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078571.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         3385 a         2824 c         3200 g         3383 t
ORIGIN      
        1 ggcgcggcgg ccatgtggtc gcttgcgtcc ggcgtctttt ctttttgcct attcctacgc
       61 atttagaacc cggtattgtc ggggatagga tgaagtattc gcttgcccct gcaaacatgc
      121 gggagaagcc ttgcagcggg cggttttcaa tcccttaaat gcgtaggttg cagctataat
      181 tagacccctg aaacagatat agccgcgcag cgggtgtatc tgtttcaggg gtgtgcaggt
      241 gatagccgca aagcggcgta agagggtgca gccctcttgc cgtctatctc tgttccagcg
      301 ggaagctcgg caatgcttcc agcagtttca gggaaatgcg ctgccgtata tcttcgtcaa
      361 tgactttctt ctttttgccg tttgcctgtc tgatctccac cgtggaaagc tcgttgatgt
      421 agtcagcata atgggccaac actttttccg tggcccacct ctcaccggca acggcggctt
      481 tgatcgtttc atagtccagc agcttttctt gtccgtccat cctgataccc ccgtcagact
      541 gattttttct atgataccat attcggaagc atggaaccag agaaaacggc gcttttctgc
      601 tggtaaattc tacgcattta gaacagcact tcggcgggga taggataaaa cacccacata
      661 ccccgctgac catgccggaa aagccttgaa atataagggc ttttacagct ctaagtgcgt
      721 aggtttccag tatcttttcc gctggcgctc ggcctctttg cggcggcgca tccgtttgga
      781 gcaggccgcg cagtatttgg cccggttaga cttcggcgtg aacgtggccc cgcactcaca
      841 gcagcgtttc acttcgtccc ggtctttggt gatctcggca tacagggccg catccagcgg
      901 gaggacagcg gccttaaacc agcggcacag aagcgaatag gaaatgctct gtacgcaaat
      961 acattcttcg ccgtcgtcca gcaacaggca gttgccgtca caatagttgc agcactcatg
     1021 cgtcagcttc cgggccttgc gatattgagg gtaattcatt cgtggaatac tcatagctct
     1081 tgcgtccatt cctttcccgg cgtcatacgc aaaatgccgt ccacattctg cttgatgatg
     1141 ccgtattcct gcatctgctt tttcgcttcg ccgtacttct catacagccg ttccttttcc
     1201 tcggccaatc gcttgtactc ggctttcagg gcgtacagat cggggagctt tttcacaccc
     1261 tgtcccttta gggcctttgc cgcagcttca tacaaaatca acgtgctttc atgctcacgc
     1321 cggaattttg ccttatccgc tgcattgcgg tattccatag ccacgggcca gagctgcttg
     1381 taggtagaaa tattctttat cagcaccgcc atatccgcaa gccggtgttc catagctttg
     1441 agggtagcgg cggcctcgtc gctggcggcg gtgatctcgg ccactttgct ttccaaatcc
     1501 gggtaggtgt ctatcccatg ttcagtcagg aaattcaaag tctttgcggc ctgcttcaaa
     1561 ttgtggagct tcgcccacct ctcatagcct gccgactgct gggccttaat gttgttttcc
     1621 agatcaatcc gcaaagaaat ccccttgcgc tctttgggcg ctctggtgat aacgcggcct
     1681 ttgatacgct ctttgatggc ttcctcggtg taggcttccc ccaatgtctt gcagcgggta
     1741 aagcgttcct gtccgggagc gcggaaagaa ataaactttc ccggcttgat ctcatagccc
     1801 tgcgcctgca tgagccgcag gaaatcatca aaatcttttg cctgcgggat agcggcgtca
     1861 atggctgctt tgagcttcgc tttccagctc gtccctttcc gctgggcgtc ccactcggca
     1921 tagctcttgc ccttactctg gccgggcttc accacggaca atccgttttc cttgcacagc
     1981 ctgtcgctgg tgcgccggat ataggcatag ctctgtttgt tggaaatgta cttgcgctga
     2041 tttaccatat ccacggcaca gaaaataatg tgattgtgga cgtggccctt atcaatatgg
     2101 gtagtcagca catagggata tttcccttgc agcacttcct cggcaagctg cttcccgatc
     2161 tcatgggcct gttccggcgt ggcttcgccg ggggcaaagg attgtatcag gtggtggccc
     2221 agattattgc ccttttggta tgcctgcgcc agcagcattt caaattcaat gtccgctgtt
     2281 tcataagagc aggcaaagga ggccaccagc agcttgccgt cagttttgtc cgggttctcg
     2341 atgtagtcaa gggctttttt cagcgttgac ttgataggat gtatctttgt aactgccata
     2401 tctgcgccag cgcccccttt atatcctcga tgtcctgtgc gtacaccggc cccataccgt
     2461 tcagcctctt tgcaatctga ttgacgttga cgccgatctt ctgtatctcg gcggtctgcg
     2521 cccggatgtc gctggtgtcc atgttgacga tatagccgtc aatgagcatc ttccgggcgt
     2581 aggctgaaaa gttcttcgta tgcagcagcg ccattttctg ttcaatcata gcccgttcct
     2641 ccggcgtcac agggacgcgc agcacgattt ttcgctttct gtttgccatg cttcaccgtc
     2701 ctttccattc aggggtttgg ggatttcccc aacaagcaaa acggacgcgg cccggcaggg
     2761 acggggcagg ccgtttttcc gggagtggct acactcccta tgcttgctac gttactgttt
     2821 ctccactaac aagacgtttc caaagcccca aactacccgc aaaatagccc tctggaaaga
     2881 aaaaagtgca aaaaaataga agctacgctg tgcagctccc gctaaaagta aaacagagga
     2941 tgccagttac acatcctccg tttcattctt cttcggcttt tcaattagta tggcgctgtc
     3001 aatcgcgccc tcgatgatcg gcaggtattc ttcggggcag agtttcagct tgtggctgac
     3061 cctttgccgc agttcgcttt cctcccgcat gatctccggg ttaaagtagc gttccacagg
     3121 aagcccgcag gctttaataa gctgtatgac aaccggcaga ctgggaatgg cccgtcgttt
     3181 tcgatgttgg caagatagcg ccagtcaata tttattttct ctgccattgc tttgcgcgaa
     3241 acgcgctttg cttgccgggc cgctttcaca tccgcgccga aagtttcaaa gccgggacaa
     3301 tcttcaatat tcgccatatc gcacctccca cttacattgt atatttcata ctctgcccgt
     3361 ggaatgttgg tatatgcacc tttcttaaag tttatatttc acattttgtt tcttttattc
     3421 ttcgagcaaa agaattataa tgtaccctgt ggaggtgtac tatggactac atgacgttga
     3481 aagaggcagg cgagaaatgg ggtgtgcccc cacgatggat aaactactat tgtgctgcgg
     3541 gacgcatccc cggcgctgta aagatggcga cgatctggct cattcccaaa actgcggaaa
     3601 agccgattga caggcgtaca aagaaaggaa aggcggttac tgaatagtat aatgaaaccg
     3661 gcgcattttg gatatggttt tgaagagaga gaaagcatgg tattttgtca cggaaaaact
     3721 ataagatgat agatggcagg ctgctccaga ccgataaaaa gtattctcat ctgaaacaga
     3781 aagaaaagat ttacacatgg atgtatgaag aagcgaaact ttatcatgac agaaatggaa
     3841 aatgcccgaa acaaagacga ggaagctgct gctgtatcag ccgtatatgg tagaattgaa
     3901 aaggccggca tctggattct ttatggtgaa gtctccagac attggataac tgctggtcta
     3961 atgcgccttt gagtatgggg tgattgattt taataccggg gaggtacggc tttttgagga
     4021 aaatattctg ggatttacat ttggcgatta ttacatttcc tgtaactgca aggacacgga
     4081 tgatgaagat atgaggaata tagacttgct gattttctac tgtcttgaac ggtattagga
     4141 ctgaatctgc tgcgcggagt gcggcaagag cgaccggagg gagtacacac aggccctgcc
     4201 tgtgtgaaaa gccataccgc aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag
     4261 gcttggggtg tccccaacaa gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag
     4321 aatatccgta aaggatattc tgatccattg cttgccagta ttatcgggat gttttgacaa
     4381 ccgctgtaat cgaaacatcc caaacattaa gaaggtgtta attattatta tcttgattta
     4441 attcgtttaa accaataata tagatacata caccatgaaa agaagcgagt atcccaatga
     4501 cggatgatta aaatccgaaa agaaatcgct ttgaaactac agggaaacta cagactgtta
     4561 tgttatcttc ttaaaatgga gggattttta tgtcgatacg aattctactt gtcgaggatg
     4621 atgatcatat ctgtaataca gtaagggcgt ttctggctga ggcaggatat caggtggatg
     4681 cctgcacaga tggaaatgag gcatacacca agttttacga aaacacttat caactggtta
     4741 ttcttgatat tatgctgccc ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga
     4801 aaaatgatac tcccattctg atgatgacag ccctgtcgga tgacgaaaac caaatccggg
     4861 cgtttgatgc agaggcagac gactatgtaa caaagccatt caagatgcag attttactaa
     4921 agcgggtgga agccctgctg cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca
     4981 ggctgacact tctgccggag gatttcacgg tactttgtga cggtacagag ctgcccctga
     5041 cacgaaaaga atttgaaatc cttttgctgc tggtgcagaa caaaggcaga accttaacgc
     5101 atgaaatcat tttgtcacgt atatggggat atgactttga gggtgatggc agcacagtcc
     5161 acactcatat caaaaatttg cgggccaagc tgccggaaaa tatcatcaaa accattcgcg
     5221 gtgtaggata ccgattggag gaatcataat aatggaaaga aaagggattt tcattaaggt
     5281 tttttcctat acgatcattg tcctgctact gcttgtcggt gtaacagcaa cactgtttgc
     5341 acagcaattt gtgtcttatt tcagagtgat ggaattacag caaacagtaa aatcctatca
     5401 gccattggtg gaactgattc agaatagcga taggcttgat attcaagagg tggcagggct
     5461 gtttcactac aataaccaat cctttgagtt ttatattgaa gataaagagg gaagcgtact
     5521 ctatgccaca ccaaatgcca atacatcaaa tagttttaga cccaactttc tttatgtggt
     5581 acatagagat gataatattt cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta
     5641 tcaagggctg acaattcggg gaattgttat gattgcgata atggttgtat tcagcctttt
     5701 atgcgcgtat atctttgcgc ggcaaatgac aacgccgatc aaagccttag cggacagtgc
     5761 gaataaaatg gcaaacctga aagatgtacc gccgccgctg gagcgaaagg atgagcttgg
     5821 cgcactggct catgatatgc attccatgta tgtcaggctg aaagaaacca tcgcaaggct
     5881 ggaggatgaa atcgcaaggg aacatgagtt ggaggaaaca cagcgatatt tctttgcggc
     5941 agcttctcat gagctaaaaa cgcccatcgc ggctacaagc gttctgttgg agggaatgct
     6001 tgaaaatatc ggtgactaca aagatcattc taagtatctg cgcgaatgca tcaaaatgat
     6061 ggataggcag ggcaaaatca tttccgaaat actggagctt gtcagcctga atgatgggag
     6121 aatcgtaccc atagctgaac cgttggacat agggcgcacg gttgccgagt tgctgcccga
     6181 ttttcaaacc ttggcagagg caaacaacca gcggttcgtc acagatattc cagccgggca
     6241 aattgtcctg tccgatccga ggctgctcca aaaggcacta tccaatgtca tattgaatgc
     6301 agttcagaac acgccgcagg gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa
     6361 atgccgcctt tttgttttga acatgggcgt tcacattgat gatactgcgc ttccaaggct
     6421 gttcacccca ttctatcgca ttgatcaggc gcgaagcaga aaaagtgggc gaagcagaaa
     6481 aagtgggcga agcggtttag gacttgccat cgtacaaaaa acgctggatg ccatgagcct
     6541 ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc tggctggatt taccgctcac
     6601 atcaacattg taaatattta aaacttaaat gattttgggc tctttgtcaa ataggactga
     6661 tagagtgaga taagaacatc taaggtagaa ttccttttct actttagatg ttttttgatt
     6721 cataggtaat cagattattt agtaagaaaa ttctggtctt cattttccga aagcttcgaa
     6781 agccataagc gactcgtttc aatgctttga tgtgggtatg cagattttct attttggcat
     6841 ttgaataagg caattcgagg gctaaacgaa tgccggattc atagtttata aaagcttttt
     6901 ttatagaatg cagttctttt ggaacggaat tgggcatcgt tcttacaagt ttaaagaaag
     6961 tgtccgcctc tttgtcgtga aacgcttgaa gaatttcttg atagtaaaaa taggcgattc
     7021 gtaattcgtc agagatcgaa agcagacgat ccaccacttc actttcggtg agatacgccc
     7081 aaccaaaatt gcgccatttg gtccatttgt cataaacaag cttgtcctgt cgtttgatca
     7141 gaagccgaga caacgccttt aattgtcgat aatatttggc ttgtgttgga tcgtttcgat
     7201 caaagctttt catgattcgt acgcgaatat cctcaaattt acgaatgaga tgtttgacaa
     7261 tgtgaaaacg atccacaacg agttttgcct ttggaaaaca ttcctttatc agagaggcat
     7321 aagaaacatt catgtccgtc acgatgacct ttaccttcaa tcgtgcgcgt cgttcaaatt
     7381 gcataaaata agaaacgaga gcctttttct tccgtgaagg aagaatatcc agtaattttc
     7441 cagtatcgcc atccatacaa gaaaaggagt agggaccgac ttgatttttc agtgaacgaa
     7501 attcatccat taataaacaa cgcggtaacc aatttctttt tacttttgtc atggggatca
     7561 gagaagcgag tgtccgagaa acggtcgacc aagaaacgcc gcaatgctta gcaattgtcg
     7621 tcattgcttg tttttcagtt agctcttcta aaattttata gcgcacttgt ttagcgataa
     7681 agcagtttct atcgacaatg taggtattcg ccgtaaaata ggtgtggcag ccacgacagt
     7741 ggtagcgttg tttcgctaaa gcaagataag tcagttggtt cgcacattta ttaagttgaa
     7801 tgagcgattt tttctttccg ttcttaacga tcatgccttc atgattcatc tgtttacaca
     7861 aaggacagcg acgaatttct ttacttaacg tcgcagaaac aacaagactc ttgatattct
     7921 taatgctctt cgattccaca tttgtgatga aaatgttttt atctttgatt ccaagtagta
     7981 gtttgatatg atgaagatca ggcatacaga acgctccttt gattgtgttg tggtgattca
     8041 attctaacgg agcttctgta tgttttgttt tttcatgctc aaaagggacc gatagaatga
     8101 ttttctatca gtcctagaaa ttatagagcc gattttgacc gacaggtata accctgccgg
     8161 tctttttttg ctgctacagg aaaactacag actggctaca gggaaagtac agatgcgctt
     8221 gccataataa caatcgtacc agccacaaat cgtagtttta ttgcaaagga ggcattcgat
     8281 caaatggaaa aaagcaacta tcattccaat gcggatcatc acaaacggca tatgaaacaa
     8341 tccgtggaaa agcgggcttt tctatgtgcg ttcattattt cgttcacagt ctgcacgctg
     8401 tttttggggt ggagactggc ttccgtattg gaggcaacac agataccgcc catccctgca
     8461 actcatacag gcagcagcac tgacgtagtg gagaatttgg aggaaaacgc tcttgccacc
     8521 gccaaagaac agggagatga acaggaatgg agcctgattt tagtgaacag gcagaacccc
     8581 atccccgcac agtacgatgt ggagcttgag caactatcaa atggtgagcg gatagatatt
     8641 cggatttctc cctatcttca agatttgttt gatgccgcaa gaactgatgg agtttacccg
     8701 attgtcgcat ccggataccg aacaacagaa aaacagcaag aaattatgga tgaaaaaatt
     8761 gccgaatata aggcgaaagg ctacacctct gcacaggcta aagcggaagc agaaacttgg
     8821 gtggccgtgc cgggaacgag cgagcatcag cttggtcttg ctgtggatat caatgcggac
     8881 ggaattcatt caacaggcaa cgaggtttat agatggctgg atgaaaacag ctatcgtttt
     8941 ggttttattc gtcgctaccc accagacaag acagagataa ccggtgtgag caacgagccg
     9001 tggcattacc gatatgttgg catcgaagct gccacagaga tgtacaacca aggggtttgc
     9061 cttgaggaat atttaaaacc agaaaaatga gaaaaggatg ttatgttatg gacagaaaaa
     9121 gattgacaca gcgtttcccg ttcctgcttc caatgagacg ggcgcagaga aaaatgtgct
     9181 tttatgcggg aatgagattt gacggctgtc gctatgcgca gacgataggg gaaaaatcgc
     9241 tttcccattt gctctttgaa acggattgcg cattatacaa ccacaatacc ggatttgaca
     9301 tgatatacca ggaaaacaag gtgttcaact taaagctggc agcaaagacc ttaaacggct
     9361 tattgataag accgggggaa accttttctt tctggttgtt cctgcatacg ccattgacaa
     9421 ttatccagcg cagaggtcac gaagtaaagg agtttccaga gccaaacagt gacgagatta
     9481 aaggggtgga tgcaaccatt tcagagggct ggattgattt aaaagtgcga aacgataccg
     9541 actgcaccta ccaaatatgg gtgaccttag atgatgagaa aatcatcggt caggtgttcg
     9601 ccgacaaaga gccccaagca ttatacaaaa ttacaaatgg cagtatccag tatgtccgtg
     9661 aaagtggcgg gatttatgaa tatgcccagg ttaaacggat gcaagttgcc ttaggtaccg
     9721 gggaaataat agattgcaag ctgctttata caaacaaatg caaaatctgt tatcccctcc
     9781 cggaaagtgt ggatattcag gaggagaacc aatgagaaaa agtgtgggca ttactgtttt
     9841 tggatgcgaa caggatgagg caaatgcttt ccgcgcttta tcgccggatt ttcatattat
     9901 ccctacgctg attagcgatg cgatatcggc agacaacgca aaattggccg ctggcaatca
     9961 atgcgttagc gtaagccata agtccgaggt ttccgaggcg acaattcttg cgctgagaaa
    10021 ggtcggggta aaatacattt ctacccgcag catcggctgc gatcacattg atacgactgc
    10081 cgccgagaga atgggaatct cggttggcac ggttgcgtat tcgccggaca gcgttgcgga
    10141 ttatgctttg atgctgatgc tgatggccat acggggtgca aaacccacca tgcacgccgt
    10201 ggcgcaacaa gatttcagat tggatcgtat ccgggggaaa gaactggggg atatgactgt
    10261 gggagttatt ggaaccggcc atatcgggca agcggtcgtc aaaaggctgc ggggatttgg
    10321 atgccatgtg ctggcctatg ataacagccg aaaaatggat gcagattatg tccagcttga
    10381 tgagcttcta aaaaacagcg atattgttac gctccatgtg ccgctttgtg cggatacccg
    10441 ccatctgatc ggtcagaagc aaattggaga gatgaagcaa ggcgcatttt taatcaacac
    10501 cgggcgcgga gcgcttgtcg ataccggggc gctggtggag gcgttggaaa gcggaaagct
    10561 gggtggtgcg gcactggatg tgttggaggg cgaggatcag tttgtttata ccgattgctc
    10621 gcagaaagtg cttgaccatc cctttttgtc gcagctttta aggatgccga atgtgatcat
    10681 cacaccccat acggcgtact acactgagcg tgtgctgcag gatactacag aaaaaacaat
    10741 caggaattgt ctcaattttg aaaggagttt acagcatgaa tagaataaaa gtcgcaatca
    10801 tcttcggcgg ttgctcggag gaacatgatg tgtcggtaaa agccgcaata gaaattgctg
    10861 cgaacattga tacggaaaaa ttcgatccgc actacatcgg aattacaaaa aacggtgtat
    10921 ggaagctatg caagaagcca tgtacggaat gggaagccga cagtctcccc gccatactct
    10981 ccccggatag gaaaacgcat gggctgcttg tcatgaaaga aagcgaatac gaaacacggc
    11041 gtattgatgt ggctttcccg gttttgcatg gcaaatgcgg ggaggatggt gcgatacagg
    11101 ggctgtttgt attgtctggt atcccctatg tgggctgtga tattcaaagc tccgcagctt
    11161 gcatggacaa atcactggcc tacattctta caaaaaatgc gggcatcgcc gttcccgaat
    11221 ttcaaatgat tgataaaggt gacaagccgg aggcgggtgc gcttacctac cctgtctttg
    11281 tgaagccggc acggtcaggt tcgtcctttg gcgtaaccaa agtaaacggt acggaagaac
    11341 ttaacgctgc gatagaagcg gcaggacaat atgatggaaa aatcttaatt gagcaagcga
    11401 tttcgggctg tgaggtcggg tgtgcggtca tggggaacga ggatgatttg attgtcggcg
    11461 aagtggatca aatccggctg agccacggta tcttccgcat ccatcaggaa aacgagccgg
    11521 aaaaaggctc agaaaatgcg atgattacag ttcccgcaga cattccggtc gaggaacgaa
    11581 atcgggtgca ggaaacggca aagaaagtat atcgggtgct tggatgcaga gggcttgccc
    11641 gtgttgatct ttttttgcag gaggatggcg gcatcgttct aaatgaggtc aataccctgc
    11701 ccggttttac atcgtacagc cgctacccac gtatgatggc cgccgcagga atcacgcttc
    11761 ctgcactgat tgacagcctg attacattgg cgttaaagag gtgataagta tggaaaatgg
    11821 ttttttgttt ttagatgaaa tgttgcatgg cgtccgttgg gatgccaagt acgccacatg
    11881 ggataacttc acaggaaaac cggtggatgg gtatgaggtg aatcgcatca tcggcacaaa
    11941 ggccgtggcg tttgctctgc gcgaggcaca aatccatgcg gctgcgcttg gctatggctt
    12001 gcttttatgg gatggatatc ggccaagaac tgcggtggac tgcttcctgc gttgggcagc
    12061 gcaaccggag gacaatctca caaaagaaaa attttacccc aatatagagc gagccgagtt
    12121 gattacaaag ggttatgtgg cctcacaatc cagccatagc cgtggaagcg caattgatct
    12181 tacgctctac cacctggata caggggaact tgtttcaatg ggaagtaact tcgattttat
    12241 ggacgaacgg tcgcatcata cagcaaaagg gataggggat gcagaggcac aaaatcgaag
    12301 atgcttgcgt aaaatcatgg aaagcagcgg atttcagtct tatcgctttg aatggtggca
    12361 ctataagttg attgatgagc cataccccga tacctatttt aattttgccg tttcataatg
    12421 aaagtattgg ttttgtatca gattttggat ttgataaaat ctatgctggt catggacaat
    12481 cttttactgg aaagtggtta aagcatcttt tgtaatacat tgcaaattat agtattcaat
    12541 ctgcccagcg gcagaaaaga aaaaaaccgc tgttgggcag acctttccca cgcccatttt
    12601 gtattttgct ggcggcggtt ttgagaaatc aaggccgccg ttcttttctg gcagcaaaag
    12661 gtgtggcggc ccataggagg atgccgctat gctgttttgc tattgccatg ctctaatcca
    12721 cttccaccac tcaaaaaaag ccttgcccct ccatgaactg aaccccaaaa gttgaactat
    12781 ttaatggact gt
//
LOCUS       DASJTC010000268        10744 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.guided.092, whole genome shotgun sequence.
ACCESSION   DASJTC010000268 DASJTC010000000
VERSION     DASJTC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.guided.092"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(204..2162)
                     /locus_tag="U4B19_002931"
     CDS             complement(204..2162)
                     /locus_tag="U4B19_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEN2078572.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="U4B19_002932"
     CDS             complement(2159..3031)
                     /locus_tag="U4B19_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEN2078573.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="U4B19_002933"
     CDS             complement(3373..4245)
                     /locus_tag="U4B19_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEN2078574.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="U4B19_002934"
     CDS             4373..4942
                     /locus_tag="U4B19_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEN2078575.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="U4B19_002935"
     CDS             5009..5746
                     /locus_tag="U4B19_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEN2078576.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="U4B19_002936"
     CDS             5977..7245
                     /locus_tag="U4B19_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEN2078577.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="U4B19_002937"
     CDS             7732..8196
                     /locus_tag="U4B19_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEN2078578.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="U4B19_002938"
     CDS             8180..8809
                     /locus_tag="U4B19_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEN2078579.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="U4B19_002939"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="U4B19_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEN2078580.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
BASE COUNT         3475 a         1999 c         2160 g         3110 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatg
//
LOCUS       DASJTC010000269         8080 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.guided.105, whole genome shotgun sequence.
ACCESSION   DASJTC010000269 DASJTC010000000
VERSION     DASJTC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.guided.105"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(134..577)
                     /locus_tag="U4B19_002940"
     CDS             complement(134..577)
                     /locus_tag="U4B19_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEN2078581.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="U4B19_002941"
     CDS             complement(795..2135)
                     /locus_tag="U4B19_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEN2078582.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="U4B19_002942"
     CDS             2404..3300
                     /locus_tag="U4B19_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEN2078583.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="U4B19_002943"
     CDS             complement(3450..4391)
                     /locus_tag="U4B19_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEN2078584.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="U4B19_002944"
     CDS             4517..5638
                     /locus_tag="U4B19_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEN2078585.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="U4B19_002945"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="U4B19_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEN2078586.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="U4B19_002946"
     CDS             7426..7992
                     /locus_tag="U4B19_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEN2078587.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         2676 a         1507 c         1599 g         2298 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat
//
LOCUS       DASJTC010000270         5735 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.guided.129, whole genome shotgun sequence.
ACCESSION   DASJTC010000270 DASJTC010000000
VERSION     DASJTC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.guided.129"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            183..1421
                     /locus_tag="U4B19_002947"
     CDS             183..1421
                     /locus_tag="U4B19_002947"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEN2078588.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            1648..2067
                     /locus_tag="U4B19_002948"
     CDS             1648..2067
                     /locus_tag="U4B19_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEN2078589.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            2072..2311
                     /locus_tag="U4B19_002949"
     CDS             2072..2311
                     /locus_tag="U4B19_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEN2078590.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(2536..3459)
                     /locus_tag="U4B19_002950"
     CDS             complement(2536..3459)
                     /locus_tag="U4B19_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEN2078591.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(3491..4255)
                     /gene="map"
                     /locus_tag="U4B19_002951"
     CDS             complement(3491..4255)
                     /gene="map"
                     /locus_tag="U4B19_002951"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEN2078592.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4413..4853
                     /locus_tag="U4B19_002952"
     CDS             4413..4853
                     /locus_tag="U4B19_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEN2078593.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(5031..5600)
                     /locus_tag="U4B19_002953"
     CDS             complement(5031..5600)
                     /locus_tag="U4B19_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEN2078594.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
BASE COUNT         1798 a          995 c         1014 g         1928 t
ORIGIN      
        1 ccttttaaat ggaataggca tagacacatt attagacgaa gaaacaattt ctgcatttat
       61 acagttttgt aaataactgt atttgttatc cctatacgat agactgctct ataaaagagc
      121 agtttattta aaccattata taaaccgacg gtctgtttga ttaaataaaa aggagagatt
      181 ttatgcaagc agaaaaaaaa ctttttaatc gtgattttat acttgttgtt ataggacaga
      241 taatttcttt atttggaaac gctatcctac gatttgcatt accgtactat ttgttaaaag
      301 aaactggttc atcagctttg tttggtattg ctacagcctg ctctttttta cctatggtta
      361 ttttgtcgct ggtaggtggt attattgctg acaggacaaa taaaagaaac attatggtca
      421 tactagattt tgtaacagca atcataattt tagtatttta tttgtcattg ggaaaaattc
      481 ctatcatacc tttgtttatt gttgctttga tgttacttta tggtatttct ggtgcatatc
      541 aaccagcggt acaagcaagt attcctctat tggtatcatc agataaactt accgtcggta
      601 atgctataat aaatcaagta aatactttag caaactttat cggaccagct ataagcggag
      661 tagtttttca attttatggt atcaatccca ttttaattgt tagtattata tgttttactt
      721 tttcagccat tatggaaatt tttatccata ttccacacca aaaaagacct cgtgaagtag
      781 gtgtaattgc agttgctaaa aacgatttaa aagatagtta tacctttttg aaaaaggaga
      841 aacccatttt tatatccgta atctttttgg tttgcatttt caatttggtt ttaagtgctg
      901 ttttaattgt tggtctgcct atcttaattt ctgaagtttt aaaaatgcca gatacactat
      961 atggtttttc acaatcagca ttatcattag gtggtttatg tggtggtatt ttaacagctc
     1021 ttgttgcttc aaagttaaaa ctaaaaaacc tttatacctt gttacttatt tgttctgttt
     1081 ctgttttact tatgggaatt tcgctatttt tagaattgcc tgcaatagtt tcctactggg
     1141 tagttacagt gatgtgcttt gtggctatgg gtgtatctac tatgtttgtt gttcaaattt
     1201 acactcttgt tcagacacaa acaccaccgc aattagtagg aaagataatg gctacgctgg
     1261 tagcagttgc tatgtgcggt caacctattg gacaaatggt ttacggtatt ttattcgatg
     1321 tatttataca agaaacatgg gttgttatgt ttgtagcttc tattgcttca atcatgattt
     1381 caatatattc aaaaagaatt tttcaaaagt tagagatata atacaagggg ttaaatagta
     1441 tgttaggcta aaaacaatat ataaaagaaa atttgaaatc tgctaaaagg atagtgttat
     1501 aaaagaaaga gtaatattgc agtatttaag gctcgcacaa atatatgttt tgtgtgggct
     1561 tttttcttac catttttctg tatttctaaa gggtataaac ggtattccgt ttatatattt
     1621 cagcattagc agaaaggggg tgagattatg aggacatctt ctttcgagca tatcgtcaga
     1681 ttacagttca attctttaat gctgattgtc attaagaaca aagtgaaaag tcgaaacaga
     1741 cagcttgcaa gacattctaa acgtgaagta ttgttttgtg aaatagccga aacgaaacaa
     1801 gttgaacgtg gaacgattga tacctgtttt tatgattgtg tatcatttaa agtcttgcac
     1861 tttacaattt atgtatctga tgaaacactt ggtacagttt tacatagact gtcagaaaaa
     1921 cagcgtagtg cgattttatt acgttatttt caaggaatga acgacagaga aatttcagaa
     1981 ctgtaccatg tatcacgttc tgctatttgc agtagaagaa ctagaggact aaggaagtta
     2041 aaaatgcttt tgagcgaaag gaagtaaaga aatgagtaaa gattatggct atccctcttt
     2101 tgaattgatt tgcaaagcgt caagcggaga tgaaatggca gttaaagaga ttttgaagtt
     2161 ttatgacggt tatatttcta aattatgctt gcgtcctttt taccactctg aaagtggtaa
     2221 aatcattatg caggttgatg aagaactaaa aggagaaatt catacagaca tgatgaaagc
     2281 aatattgaaa tttgaaatcg gagtgaagta atcaactgta taaaaaaatc gggagataca
     2341 cagtttcgtg ctatctccct tagtagtttt acggtcatat caaggctcat tcaatcgtgg
     2401 taaattcgtg gtaagatgag tgtttttcta tacttcaaaa tgcttgaatg tatagtgttt
     2461 atgcggataa ttgaaaatta gatataaaat ttcaaataaa ttactgatat aaatttacaa
     2521 taagaactct tgtttctact tttttttatg agaaaattta gttttcaatt tattgaacca
     2581 ttttcggctt ggatctcgac gttttttgat ttgatgaccc gtcacgtatt cttgaacaac
     2641 tgcattgatg attccgccca aaatgatgat cgtagcagca aaattaagcc aaagcatgag
     2701 tacaataaaa cttcctatga tctgataacc gctgatcctc gttgcaaaat acctagcata
     2761 gatactaaac acttgtgata acagcatcca gccgacagta gcgaagatcg tgcccggaat
     2821 gatcgtcttc aacttcaact gagcattagg gacaattaaa taaatcaaga acatgatgac
     2881 aaataaagct accaatgtga ttggccactt caaagtttgg aactgatcga taaagttcac
     2941 gggtatttgt acgattggct gaatagcatc caaaatcagc tgtcccaacc caagaacgat
     3001 agttacccca ctaatagcaa tcatgaacaa gacaatgaca attagtgaga agaaacggac
     3061 gatcaaaaaa ttttttctat tttctacgcc atacgcttta ttcatcgcaa tttgcaaagc
     3121 attgatactt tgacttgcag accataaagc cgctaatgca gaaacggata acaagcctcc
     3181 tgaactttgt gtcaaaaggt ctttgattgc aggaccgata aactggtaaa cggtctcagg
     3241 aatcacttct cgtaaatagg tcagtacagt ctcttgatcg atgtggagaa acggtaaaat
     3301 attaccaaaa gcaataatca atggaaataa agacaagagt aaataataag caacgacgac
     3361 agaggtcgta ctcatttctg aatcagtgat gcgcttttga gtcgtctcaa taaagcgcat
     3421 cagattttca ttttgtttta ttttgttcca aactttcatg tacctctccc gtacctattg
     3481 actggataga tcaataagtg ccttcttctc cttgtgatgt caaaattctt ggaccatctt
     3541 tagtaatagc caatgtatgt tcatattggc aagaaagtcc gccgtctctt gtatacgcag
     3601 tccagccgtt aggatccatt ttcattttcc aagttccggt attaaccata ggttcgatcg
     3661 tgataaccat gccttctttc aaccgcaacc cttttccagc ttctccgtaa tgaggaactg
     3721 ctggttcttc atgaatcgta ggaccaacgc catgaccaat aaaatcacga acgatcgcta
     3781 gattttcgcc ttctacataa gtttggatag catgaccgat gtcaccaatc cgattcccta
     3841 cttgtgcttg ctcaataccg atgtacaaag ctttcttcgt tacttccatc aatcggtcga
     3901 tctcaggtgt agattctcca actacatagg cccaacaaga atcagaaatg ccgcctttca
     3961 aatcgataca catatcgact tttaccaaat caccgttttt caaaggcttt ttacgaggga
     4021 atccatgaca aatttcatca tttatactga tacaagtcgc atattcatac ccttcaaagc
     4081 caatttgcgc agcaattccg ccatgactct cgatatagtt tctgacaaat acctcaatat
     4141 cccaacttgt gattcctggt tttataaaat cacgcagatt tttatgtaca tctgctaata
     4201 aagctccaga ttctgccatc atttcaattt cacgtgctga ttttaaagta atcatatttt
     4261 cgtctccttt ttacaaatcc ctctcatttt aacatagcgg catacattta gccaaatgac
     4321 attccccagg tgtctctagg ttttattttc ttttcaatga tgttttggta taattcataa
     4381 ttgttaaata attataaacg aggaggcaca tcatggcttt agcaaaaatc gtatatgcta
     4441 gcatgacagg aaacactgaa gaaatcgccg atattgttgc agaagcattt gaaaatctgg
     4501 atatcgaagt cgaaatcaac gaatgtaccc aagtagatgc agatgaattc gaggaggcag
     4561 atatttgcgt agtagctact tatacatacg atgatggcga tctgcctgat gagatcgtag
     4621 acttttatga agacttgcaa gaattggatc tattgggaaa aatttacggt gtctgcggat
     4681 ctggtgatac tttttacgat gaattctgca aatcggtgga tgatttcgat gctgcttttg
     4741 caaaaacagg cgcatcaaaa ggagccgaaa atgttaaagt ggatctgaat gctgaagaag
     4801 aagatatcga aaatttagaa gctttcgcta aagaactagt cgcaaaaatt taatatactt
     4861 gcaataaaag agccatccag atttattcaa tctgtatggc tcttttattc attgcattca
     4921 tggaagagtt cttcgggagt taaaaatgca tacgctgtaa gaaaaacgaa cttttcctta
     4981 ttttgacaca tctcttgtta cctactgatt tgtggaactt ttgagtatat tcaatcgatt
     5041 tcgattggtt ctttgaattt caagatagca tctatactgt cttggatcat cttttcaatc
     5101 tgttcatcag ttggatggat gatgcttgaa gacatcaaag ctgcgatccc agtagctatg
     5161 atccatgtcc cattatacaa agattcgatc tgctcttctg aaagattcac ataagctgga
     5221 tcgcgtttca ctgtattcac aaagtaagta taagagaaat tctgcatctc ttttcctcca
     5281 ccatattctt ctaagtacag tgaacgatac aacttcttct ctcttcttgc aaaatggata
     5341 taattaaaag caagatcgat aatcgtattc cctgtatgct caacagtgaa cacttcatat
     5401 tgtaaatgat catagatctg ctcaaaaaga gcttgtctca agtcatccat atttttaaac
     5461 tcaagataaa tcggttgcgt ggaacaattc attttgttcg cgatgtttct cgcagtaaat
     5521 cgtgtaaatc catcggtggc agctacttca taagcagctg ccaaaatctg ttctctagta
     5581 atcgtctttt ttcttgccat attttttaat ctccttaatt tttttataac gcttccacca
     5641 ttgattaatt tcattgtaac atgttttttt tgatatgcaa aattagtttt tgattttctt
     5701 cacaagctga aatagcaagt agtaacatat atcat
//
LOCUS       DASJTC010000271         3947 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.guided.154, whole genome shotgun sequence.
ACCESSION   DASJTC010000271 DASJTC010000000
VERSION     DASJTC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.guided.154"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            complement(39..341)
                     /locus_tag="U4B19_002954"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="U4B19_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1055..1345)
                     /locus_tag="U4B19_002955"
     CDS             complement(1055..1345)
                     /locus_tag="U4B19_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078595.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1747..1983
                     /locus_tag="U4B19_002956"
     CDS             1747..1983
                     /locus_tag="U4B19_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEN2078596.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1990..3942
                     /locus_tag="U4B19_002957"
     CDS             1990..3942
                     /locus_tag="U4B19_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEN2078597.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT         1381 a          667 c          685 g         1214 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagattt aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag
     1561 tatcaaattg caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta
     1621 atttacacca caaaatgtaa gttaaaatgt atataaaaat gcgaacgtga aataattatt
     1681 gtaggaatgc aaattggtgt gttaaaatgg ttacaattta ctaagcaaaa aggggtgcat
     1741 tttcctatgc aatttcttga gccaaaaaat aagaacgcta agtctgtgga ttgggaaata
     1801 tcagagcaag taagggtgat agttaagcaa tacgctgaat atgcagagcg tactgaaagc
     1861 gaagcagtgg atgaattctt attgaacatt ttagacgata agaagtttat tgaatggatt
     1921 gccaacaagc gaagtaataa gcggattgtt gaaaaaatgg gtatcaaaga tagagtgggt
     1981 tgatagacaa tgaccaagct taaaagatta gccacgaagg aagatgaaat tgtagatgtt
     2041 aaaattccga tttcaaacga agaattaaaa gatagggcta aacaatatga tttattaact
     2101 ccaaaacgat ttgcaacaag gtataacaaa atgttgttct tgccaacgtc ttttaaatgg
     2161 aacggttctg aatatcccat tcaatataat tactgcatca atccgttttg ttgtaatttt
     2221 gggaaagaac agcacaaatt caaagatgta aaagggaaac caagtcgata taaaatgact
     2281 ggtagttcca aagataaagg tcataaaggt atgtactgca atgacaatcc tattggcaga
     2341 ggtgtatcgc aaaactgtac agtaacccct ttatctaatt ggtctgttgt tgaagaaatt
     2401 aaacgcctta tagaaatcaa tagcatccaa gatgttgagc cagattatca atttcataaa
     2461 gaaggctgct cggaagaaga atcaacccca ttcaatgagc cgaagcaatt ttacaaaagg
     2521 gggaaaagta ggggcaaatc tcaacgctat caatgtaagg cgtgtaagaa gttcactaat
     2581 gttctaccaa aaagagaaga aaccactact tatcaccaac aaaagaacac cattcttcca
     2641 atgttcgcta agatggttgt tggaagggtt tcagtcagtc gtacttgtga tattttagga
     2701 attggagttg gaacctacta tcataagctg gagtggctgt ataggcgttg tttggagttt
     2761 ttagaacgat atgagacaca accccttcaa acaaagaaat ttaacgagat gtggcttaat
     2821 acggacaaga tgcactacta cctaaataac gtccgtaaga aaggacaagg ttcaaagaaa
     2881 tatacggggt ttgaagacct taatatgcaa acgtatatag ttgtatctgc tgaggtctta
     2941 tcaagatatg tgttccgttc tgatgttgcc tatgattgga atatttcaat ggatgaatta
     3001 aatgaagata caagaaaatt caaagaagac catctcaata cttttagtcg taaaaatgat
     3061 aggttagatt ggtcttatta tccacaagaa ccatccgcta acgatagcga aaataggaat
     3121 gcttatcttc acgaattagg gaaaattact aataggagta gatttgtcga tggtttaaat
     3181 gttgatgctc cttatactac aactgctcac tattggttaa ttaaacaaat ggttaatgcc
     3241 gacgaatggc gaatgattag tgatgatgac ttctctatta gaaacgcctt ttatagagta
     3301 ttcacgaaag aattaaggtt gagcgatgca catcatttta tttgccaagt taacaagacg
     3361 aaaagcagaa aacaatgctt aaaagaattc ggacaagcaa aagcagaact gctcgattgg
     3421 ggagatatca gaggattcaa aacaaagttt ctacggactt tggcatctca ttatctaact
     3481 gaattactaa caagtcatca attccacgaa gaggctatta gtaaagatgg agaaagatat
     3541 cgcaaatatg ccgataaccc tatcaaacat ccattagcca ctaaggataa aggattttat
     3601 tcagttgatt gtagaacgga tttatctgct ctggaaccaa acgaaatcgc aaagatgcta
     3661 ttaaatgtta atgaccattc gaccaatagc tttattcaac agataagacg ttatatatca
     3721 tctcttgaaa gaccattaac aacagctcgt ggtgataaga agagctatat ttacgctaac
     3781 ttcaatccga agtatgctca atttgcgatt acaattctaa gaacttacta caacttttgt
     3841 cgccctttta aatcagcgga taaaaaggtt ttgacacctg ctcaaagatt agggattaca
     3901 gataaacaat ttgattggaa agatataatt tatttcaaat gacaaaa
//
LOCUS       DASJTC010000272         3796 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.guided.159, whole genome shotgun sequence.
ACCESSION   DASJTC010000272 DASJTC010000000
VERSION     DASJTC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.guided.159"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            110..193
                     /locus_tag="U4B19_002958"
     CDS             110..193
                     /locus_tag="U4B19_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEN2078598.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="U4B19_002959"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="U4B19_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEN2078599.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="U4B19_002960"
     CDS             complement(1318..2181)
                     /locus_tag="U4B19_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEN2078600.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="U4B19_002961"
     CDS             complement(2183..2455)
                     /locus_tag="U4B19_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEN2078601.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="U4B19_002962"
     CDS             complement(2472..2681)
                     /locus_tag="U4B19_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEN2078602.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="U4B19_002963"
     CDS             complement(2759..3655)
                     /locus_tag="U4B19_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEN2078603.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1374 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccctt
//
LOCUS       DASJTC010000273          881 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.guided.243, whole genome shotgun sequence.
ACCESSION   DASJTC010000273 DASJTC010000000
VERSION     DASJTC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.guided.243"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            268..816
                     /gene="aac(6')"
                     /locus_tag="U4B19_002964"
     CDS             268..816
                     /gene="aac(6')"
                     /locus_tag="U4B19_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEN2078604.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          312 a          128 c          185 g          256 t
ORIGIN      
        1 ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc tatatcatga
       61 tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct attttgaata
      121 aagtgtttct gtataatttt ttattttata agtaagataa ataatgaaag aatagagata
      181 ctgattggta gctaaagatt tttgagcgag aacatttcgt tttttcaaaa ggactgttag
      241 aataagcaag aacaaggggg aagacgtatg ataatcagtg aatttgaccg taataatcca
      301 gtattgaaag atcagctttc tgatttactg agactgactt ggccggaaga atatggagac
      361 agctcggcag aagaagtaga agaaatgatg aatccagaac gaatcgcggt agcagcggta
      421 gaccaagatg agttagtagg atttattggt gcaatccctc aatacggtat cacaggttgg
      481 gaattgcatc cattagttgt agaaagctcc cgacgaaaga accaaatagg tactcgatta
      541 gtcaattact tagaaaaaga agtagcttcc agaggaggaa tcacgattta tttaggtacg
      601 gatgatttag accatggaac aacgttaagt caaacggacc tgtatgagca tacatttgat
      661 aaagtggctt ccatccagaa ccttcgtgaa catccgtatg aattctatga aaaattaggt
      721 tataaaatcg taggtgtctt accaaatgca aatggctggg acaaaccgga tatttggatg
      781 gcaaaaacga ttattcctcg accaaattct caataataaa aagaggttgt gacagaagta
      841 atcagcttca agaaataaga aggaatttac gaaaattact t
//
LOCUS       DASJTC010000274          544 bp    DNA     linear   BCT 14-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE5970
            SAMN38756303-rid20573403.guided.266, whole genome shotgun sequence.
ACCESSION   DASJTC010000274 DASJTC010000000
VERSION     DASJTC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38756303
            Sequence Read Archive: SRR27167975
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (13-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 13-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002028139.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002028139.1
            Genome Coverage       :: 144x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/14/2023 03:56:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,965
            CDSs (total)                      :: 2,903
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 62
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 30 of 72
            Pseudo Genes (incomplete)         :: 26 of 72
            Pseudo Genes (internal stop)      :: 23 of 72
            Pseudo Genes (multiple problems)  :: 6 of 72
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38756303-rid20573403.guided.266"
                     /isolate="VRE5970"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark: Copenhagen"
                     /lat_lon="55.67 N 12.57 E"
                     /collection_date="2021-02-05"
                     /collected_by="Hospital, Capital Region, Denmark"
     gene            279..>544
                     /locus_tag="U4B19_002965"
     CDS             279..>544
                     /locus_tag="U4B19_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEN2078605.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCK"
BASE COUNT          211 a           54 c          107 g          172 t
ORIGIN      
        1 atactaagta gttcagtttt ggagtacaaa atgacaaaat gtacttcaaa atggactact
       61 tttttcattt taacttacat accaaattac atacaaaatg atttataaaa tgcaattgaa
      121 aatgataatt gaatgaaaat ttgtgatttt gtggtattat gataatataa cttatagaaa
      181 aaattgctta ttcaactaac gggcaggata atgagaaaag gttttttgat agaaataaga
      241 aattagatgg taaattagtt aaaaaggctg tgttataaat gaaagtttct ttgattgctg
      301 cgatggataa gaatagagtg attggcaaag agaatgacat tccttggagg attcccaagg
      361 actgggaata tgttaaaaat actacaaagg gacatccgat aatattaggt aggaagaacc
      421 ttgaatcaat cggaagagcc ttacctgaca gaagaaatat tattctgacg agagataagg
      481 ggtttacctt taatggttgt gaaattgttc attcaataga agatgttttt gagttatgta
      541 aaaa
//
